PH27092A - Known and selected novel arylmethylenyl derivatives of thiazolidinones imidazolidinones and oxazolidinones useful as antiallergy agents and antiinflammatory agents - Google Patents

Description

. NE -1-

KNOWN AND SELECTED NOVEL ARYLMETHYLENYL DERIVATIVES

OF THIAZOLIDINONES, IMIDAZOLIDINONES AND

OXAZOLIDINONES USEFUL AS ANTIALLERGY AGENTS

AND ANTI INFLAMMATORY AGENTS

This is a division of application Serial No. 38699 filed on May 25, 1989.

BACKGROUND OF THE INVENTION it is now found that compounds from among known and selected novel arylmethylenyl derivatives of thiazolidinones, oxazolidinones or imidazolidinones have activity useful for treating allergies or inflammation.

Thus, the present invention is for selected novel compounds, as well as, pharmaceutical compositions and methods of use for known compounds and the selected novel compounds of the formula (I)

Yy

SNCS .

X— , and pharmaceutically acceptable salts thereof; wherein Ar is (i) phenyl unsubstituted, (ii) phenyl substituted by from one to three of lower alkyl, lower alkoxy, hydroxy, halogen, trifluoromethyl, NRjoRi) wherein R,o and R;, are independently hydrogen or lower alkyl, NOg2, mercapto, or lower alkylthio, (iii) naphthyl; (iv) penzofuranyl, (Vv) pbenzolblthienyl. (vi)

: Vas -2- 2- or 3-thienyl, (vii) 2- or 3-indolyl, (viii) 2- or 3-furanyl, or (ix) 2-, 3-, or 4-pyridyl

Y and Y, is oxygen or sulfur;

X is sulfur, oxygen, NH or NCH, and

X, is NH or NCH, with the proviso that when Ar is substituted phenyl, the substitutents are either not 3,5-di(isopropyl)-4-hydroxy or if Xx, = NH, X = 8S, ¥ = S not 3,5-di(t-butyl) together with 4-hydroxy.

Japanese Application No. 84-133826 as found in Derwent

Abstracts No. 87-076379/11 discloses new 3,5-diisopropylbenzylidene-2,4-imidazolidinone and the analogous thiazoles with antiallergic and tyrosine kinase inhibiting activity as well as other heterocyclic containing compounds. EP No. 211,670A as described in Derwent 87-051809/08 describes compounds excluded by the proviso above having a 3,5-dliisobutylbenzyledene substituent useful for treating inflammation, ischaemia induced cell damage and arthritis. Thus, known heterocyclic compounds are not as closely related as these named here and are excluded from the present invention.

A French Patent No. 2,169,334, C. H. Boehringer Sohn,

October 12, 1973 describes a series of N-substituted oxazoles as antiarthritics, antirheumatics, and immunosuppressants having the formula 5 . OF

QO)

S which are not included :n -he present invention.

Copending Philippine application Serial No. 39605 filed November 28, 1989 is for novel compounds having a 3,5-di-tertiarybutyl-4-hydroxyphenyl moiety so its oo 3 -3- disclosure relating to references in the Background of the

Invention is incorporated herein by reference.

The compounds of the formula I have a hitherto unknown pharmaceutical activity useful for treating allergic or inflammatory conditions or diseases. The invention compounds are now also found to have activity as inhibitors of s-lipoxygenase and/or cyclooxygenase providing treatment of conditions advantageously affected by such inhibition including inflammation, arthritis, pain, pyrrhia, and the like. Thus, the present invention is also a pharmaceutical composition for diseases or conditions advantageously affected by activity inhibiting singly or together S5-lipoxygenase and cyclooxygenase or method of use therefor. Therefore, the present invention is particularly a pharmaceutical composition for the treatment of allergy or inflammation which comprises an antiallergy or antiinflammatory effective amount of a compound of the formula I together with a pharmaceutically acceptable carrier. Further, the present invention is a method for treating allergies or inflammation in subject suffering therefrom which comprises administering a compound of the formula I in unit dosage form.

The present invention is also the novel selected compounds of the formula I noted hereinafter having activity heretofore unknown for analogous compounds.

Among the known compounds for the compositions or methods of use of the present invention the following are referenced for compounds listed for the Examples of the invention:

Example No. Reference 1 J. Chem. Soc., pp. 958-65 (1946) 2 J. Org. Chem., 26, D. 1326 (1961) 3 Chem. Ber., pp. 459-69 (1941) 4 J. Chem. Soc., pp. 3547-52 (1950) 5 J.c.S., p. 1165 (1954) 6 J. Org. Chem., p. 1326 (1961) 7 U.s. pat. No. 3,843,797/JACS, p. 2357 (1951)

: rath —3-

Cxample No, Reference 8 C.A. 89: 100767m, JACS, p. 2357 (1951) 9 Tet., p. 2781 (1969) 10 Canadian J. Chem., p. 2089 (1959) 12 Ger. Pat. DE 3,433,475/C.A. 103, 928404 17 J.0.C., p. 32 (1956) 18 Tet., p. 278L (1969) 19 Tet., p. 2781 (1969) 20 Tet., p. 2781 (1969) 21 J. Ind. Chem. Soc., p. 77 (1984) 22 J. Ind. Chem. Soc., p. 77 (1984) 26 See Example 8 above 28 Int. J. Sulf. Chem. Part A, p. 261 (1972) 29 C.A. 76 (5), 25152h 30 C.A. 79 (25), 143522p 32 C.A. 76 (5), 25152h 34 C.A. 79 (25), 143522p 36 Biol. Mem. 9, 200 (1984) 38 Zh. Org. Khim, p. 212 (1983) 40 J.C.S. (1), p. 385 (1984) 41 C.A. B89: 100767n 42 Chem. Phar. Bull., p. 1619 (1986) 43 Int. J. Sulf. Chem. Part A, p. 261 (1972) 44 J. Pharm. Soc. Jap... p. 154 (1956) 45 Ger. Pat. DE 3,433,475 48 J.C.S., p. 3547 (1950) 49 C.A. 47, 9543a 50 C.A. 47, 9543a 68 C.A. 71 (17), 81253a 69 Zh. Ob. Khim 26, p. 3092 (1956) 71 Ukrain Khim 2h. 16, p. 545 (1950) 83 Chem. Pharm. Bull., p. 1619 (1986) 86 Am. Chem. J., p. 368 (1911) 87 JACS, p. 1606 (1913) 90 JACS, p. 1606 (1913) 91 Monats, p. 352 (1961) 92 Chem. Pharm. Bull., p. 1619 (1986)

oY 7 -5-

Example No. Reference 96 Roecz, Chem., p. 1381 (1984) 103 U.S. Pat. No. 3,017,270 104 Egypt J. Chem., 26, 301 (1983) 105 French Pat. No. 1,604,530

The selected novel compounds of the present invention are found in Examples 11, 13-16, 51-53, 55-63, 64, 70, 72-79, go, 82, 88, 97, and 99-101, 106-111.

Accordingly, the present invention is (a) a selected novel compound as in the noted examples heretofor or a salt thereof; (b) a method for preparing a novel selected compound or a pharmacologically acceptable salt thereof; (c) a pharmaceutical composition comprising a compound of formula (I) or a physiologically acceptable salt thereof and a pharmaceutically acceptable carrier therefor; (d) a method for preparing such compositions; (e) a method for the inhibition of histamine by use of a nontoxic, effective, inhibitory amount of a compound of formula I or a physiologically acceptable salt thereof; (f) a method for the prophylaxis or treatment of disease or condition in a mammal, including man, comprising the administration to said mammal of a nontoxic, therapeutically or prophylactically effective amount of a compound of formula I or a physiologically acceptable salt thereof; (g) a method for the prophylaxis or treatment of any individual condition described herein, in a mammal, including man, comprising the administration to said mammal of a nontoxic therapeutically or prophylactically effective amount of a compound of formula I or a physiologically acceptable salt thereof; (h) a method for the prophylaxis or treatment of allergy or inflammatory condition in a mammal, including man, comprising administration to said mammal of a nontoxic, effective, antiallergic or antiinflammatory amount of a val ¥ -6- compound of formula I or a physiologically acceptable salt thereof; (1) a novel compound as set out in the examples noted heretofor or a physiologically acceptable salt thereof for use in medicine, especially as defined in (E)-({h) above: (J) use of a compound of formula I or a physiologically acceptable salt thereof in the manufacture of medical therapeutic agents, particularly those for use as defined in (£)-(h) above; and (k) any novel feature described herein.

SUMMARY OF THE INVENTION

The present invention is a pharmaceutical composition and method of use as defined above for compounds of the formula I as well as the novel compounds also as noted above.

A preferred embodiment of the present invention is the composition or method of use for a compound of the formula (IT) 0 ase : 0; 2 NH mR x, .

Rj Ry or pharmaceutically acceptable salts thereof; wherein X and Y are as defined above, R,, R,, Ry, Ry and Rg are independently

H, lower alkyl, lower alkoxy, OH, halogen, CF,3, NR|gR,, wherein

Ris and R,, are as defined above, NO,, mercapto, or lower alkylthio, wherein of from one to three of R,, Ryo, Ry, Ry, and Rg are other than hydrogen, but excluding Ry, R,, Ry, Ry and Rg which are 3,5-di(2-isopropyl) and 4-OH or which are 3,5-di(t-butyl) and 4-OH when X is NH, or both X and Y are S.

x1 & -7-

A preferred embodiment of the formula II of the present invention is the composition or method of use for a compound of the formula (III) 0 fy

Mes

R= O Rs s—& ITI

AN S

Ry Ry or pharmaceutically acceptable salts thereof: wherein Ry, Ry,

Ry: Ry and Rg are as defined above.

Another preferred embodiment of the formula II of the present invention ls the composition or method of use for a compound of the formula (IV)

J

RN

AS

MS rR, O 77s 4

NX 2 0 Iv

Rj Ry or pharmaceutically acceptac.e salts thereof; wherein Ry, Ry,

Ry, R4 and Rg are as def:ned above.

Also among the prefer-ed embodiment of the formula II is the composition and methcd =f use for the compound of the formula (V)

. ee

CV pe -8-

I 0

MN rR, O Rr {

L S

LAN © 5 J 3 Ry or pharmaceutically acceptable salts thereof; wherein Ry, Ry,

R;, Ry and Rg are as defined above.

Another preferred embodiment of the formula II is the composition and method of use for the compound of the formula (VI) 0

MI x r NH rR,— OO Rs

L .

SIX an 1

Rj3 Rq or pharmaceutically acceptable salts thereof; wherein R;, Rj,

R,, Ry and Rg are as defined above.

Another preferred embodiment of the formula II is the composition and method of use for the compounds of the formula (VII) 0

RIN

MS

Re O J Rs [

L J X VII

/~NX 0

R; Ry

VA m. -9- or pharmaceutically acceptable salts thereof; wherzin X is

NH or NCH, and Ry, Ry, Rj, Ry and Rg is as defined above.

Another preferred embodiment of the formula II is the composition and method of use for the compounds of the formula (VIII) 0

RN

= 1 ( NCH3 rR, OJ Ps HN

Og" wm, vir

Rj Rg or pharmaceutically acceptable salts thereof wherein Ry, Rj,

Ry, R4, and Rg are as defined above. And finally, a preferred embodiment of the formula II is the composition and methods of use for the compounds of the formula VIIIA 0

RN

( NH re O J Rs o—4 aN 0 VILIA 3 Ry or pharmaceutically acceptable salts thereof: wherein Ry, Rg.

R,, R4 and Rg are as defined above.

The most preferred embodiment of the formula II is the composition and method of use for the following compounds which inhibit the release of histamine from human basophils as described hereinafter in the HHB assay stimulated with anti IgE at an ICg, less than 20 uM. 5-((2,5-dimethoxyphenyl)methylene]-2-thioxo-4- thiazolidinone, 5-(2,4,5-trimethoxyphenyl)methylene]-2-thioxo=4- thiazolidinone,

> -10- 5-((3,4,5-trimethoxyphenyl)methylene]|-2-thioxo-4- thiazolidinone, 5-{(3,4-dichlorophenyl)methylene]-2-thioxo-4- thiazolidinone,

S-{(3,4-dimethoxyphenyl)methylene]-2-thioxo-4- thiazolidinone,

S-{(3,5-dimethoxy-4-hydroxyphenyl)methylene)-2-thioxo-4- thiazolidinone,

S-{(3,5-dimethyl-4-hydroxyphenyl)methylene)-2-thioxo-4- thiazolidinone, 5-{ (S-bromo-4-hydroxy-3-methoxyphenyl)methylene)-2- thioxo-d4-thiazolidinone, 5-( (4-methoxyphenyl)methylene)-2-thioxo-4- } thiazolidinone, 5-[ (S-hydroxy-4-methoxyphenyl)methylene)-2-thioxo-4- thiazolidinone,

S-{(3,5-dimethoxyphenyl)methylene]-2-thioxo-4- thiazolidinone, 5-[(4-hydroxy-3,5-dimethoxypheryl)methylene]2,4~ thiazolidinedione, 5-[ (4-hydroxy-3-methoxyphenyl)methylene]-2,4- thiazolidinedione, 5-( (4-hydroxy-3-methoxyphenyl)methylene]-2-thioxo-4- oxazolidinone, 5-{ (4-hydroxy-3,5-dimethoxyphenyl)methylene]-2-thioxo-4~ oxazolidinone, 2-thioxo-5-[(3,4,5-trimethoxyphenyl)methylene]-4- oxazolidinone, 5-{ (4-hydroxy-3,5-dimethoxyphenyl)methylene]-2-thioxo-4- imidazolidinone.

Novel compounds which inhibit singly or together 5-lipoxygenase and cyclooxygenase are found by determining percent inhibition in ARBL/ARBC Whole Cell S5-lipoxygenase and

Cyclooxygenase Assays and Carrageenan-Induced Rat Foot Paw

Edema-2 (CFE-2) Assay described hereinafter.

Among the preferred compounds as determined in the

ARBL/ARBC and CFE-2 assays are the following:

. 0 7 ~11- 2,4-thiazolidione, 5-[(3,5-bis(l,l-dimethylethyl)-4- hydroxyphenyl)methylenel-, (E}- d-oxazolidinone, 5-{(3,5-bis(l,1-dimethylethyl) 4- hydroxyphenyl }methylene}-2-thioxo- 2,4-oxazolidinone, 5-((3,5-bis(l,l-dimethylethyl)-4- hydroxyphenyl methylene] -, 2,4-oxazolidinedione, 5-((4-hydroxy-3,5-dimethoxy- phenyl)methylenel-, and 4-thiazolidinone, 5-[ (4-bromophenyl)methylene]-2-thioxo-.

The most preferred novel compound of the present invention now found having activity which inhibits s-lipoxygenase and cyclooxygenase are 5-{[3,5-bis(l,1l-dimethyl- ethyl)-d-hydroxyphenyl]methylene]-2,d4-thiazolidinedione and 5-((3,5-bis(l,l-dimethylethyl)-4-hydroxyphenyl]-2-thioxo=4= oxazolidinone.

Thus, the present invention is also a pharmaceutical composition for the treatment of conditions advantageously affected by the inhibition of S-lipoxygenase and/or cyclooxy- genase which comprises an amount effective for the treatment of the condition of a compound of the formula I or various preferred embodiments and the pharmaceutically acceptable acid addition or base salt thereof together with a pharmaceutically acceptable carrier. The condition is meant to include, for example, arthritis or other inflammatory diseases, allergic conditions or diseases, pain, fever, and psoriasis, but now preferably inflammatory diseases.

The present invention is also a method for treatment of the condition as noted above in a mammal, including humans, suffering therefrom with a compound of the formula I or the pharmaceutically acceptable acid addition or base salt thereof, in unit dosage form. The invention also provides for use of any such compound of formula I or particularly various preferred embodiments or salt thereof in the manufacture of medical therapeutic agent. pharmaceutical composition or use of the compound or salt of formula I is meant to include treatment understood to be prophylactic pertinent to the foregoing named condition.

A DETAILED DESCRIPTION OF THE INVENTION

In the present invention "lower alkyl" is alkyl of from one to six carbons, inclusive, and means methyl, ethyl, propyl, butyl, pentyl, or hexyl and isomers thereof.

S "Lower alkoxy" means methoxy, ethoxy, propoxy, butoxy, pentoxy, or hexoxy and isomers thereof. "Lower alkylthio" means methylmercapto, ethylmercapto, propylmercapto, butylmercapto, pentylmercapto, or hexylmercapto and isomers thereof. "Halogen" is chloro, bromo, fluoro, or iodo.

Generally, the selected novel compounds of formula I as well as the known compounds are prepared by processes that : are known or are prepared by processes analogous to those that are known from known starting materials or starting materials that can be prepared by known methods. For example, the following starting materials are obtained as follows: 3-Bromo-4-hydroxybenzaldehyde and 3,5-dibromo-4-hydroxy- benzaldehyde are prepared according to Paal, Chem. Ber. 28 (1895), 2407. 4,5-Dimethoxy-2-hydroxybenzaldehyde is prepared according to Robinson & Head, J. Chem. Soc. (1930), 2440. 3-Methoxy-5-hydroxybenzaldehyde is prepared according to

Ben, et al, J. Org. Chem. 59 (1985), 2238. 3,5-Diisopropyl-4-nycdrsxyoenzaldehyde and 3,5-dimethyl 4-hydroxybenzaldehyde are prepared according to U.S. Patent

No. 4,009,210. 3-(dimethylamino)meznv.-1-hydroxy-5-methoxybenzaldehyde is prepared according to Hemetsberger, Monats. Chem. 102 (1971), 1110. 3-Methylhydantoin is prepared according to Giller and

Moodie, J. Chem. Soc. Per< II (1980), 1752.

A scheme for preparaz:on of the compounds of formula I above is as follows:

Lo : 7 -13-

Yy Y,

RrlHO + A —_— NO x —& x—

Y Y

2 31 1

Scheme 1 - . wherein Ar, X,, X and Y are as defined above.

More particularly, the present Invention includes compounds of type 1: oo 0 aN

SS

Ra—O 5 Rs o—&

SN 0(s)

Ry Ry . 1 vhere R,, R,, Ry, R, and Rg are as defined above. These compounds are named as substituted 5-phenylmethylene-2- thioxo-4-oxazolidinones, or S-phenylmethylene-2,4- oxazolidinediones,

CHOLINE SALTS OF ARYLMETHYLENYL THIAZOLIDINONES

AND OXAZOLIDINONES ) : Certain thiazolidinones and oxazolidinones are acidic (pKa 3-6) due to the presence of a tautomeric proton on the heterocyclic ring:

-. | ly 134A \ o—4 Po § 8 ‘ 8H : }

O

NH ens N 84 § t 0 OH

These compounds can focm salts with inorganic and oryanic bases, including choline derivatives.

If the parent oxazolidinone or thiazolidinone and choline free base OC a choline derivative (such as choline bicarbonate or choline chloride) are combined in a suitable solvent {such as an alcohol, water, or an alcohol/water solution), the choline salt can generally be obtained oy precipitation from solution or by evaporation of the reaction solution.

A general method of preparation of these compounds is the aldol condensation of an aldehyde 2 with an active methylene compound 3 (Scheme 1 above).

This condensation can be carried out in alcoholic solvents in the presence of a base such as ammonia, ammonium salts, or piperidine, or with mineral acid

: . of)

MT

-14- scheme 1' 0 0

ArCHO + A —_— NO o—& o—&

S S

2 I wherein X 1s O

X, is NH

Y is S catalysis. A particularly favored procedure is the use of anhydrous sodium acetate in glacial acetic acid, with heating at reflux for 1-24 hours. References to this procedure include: G. R. Newkome and A. Nayak, in Advances in

Heterocyclic Chemistry, A. R. Katritzky and A. J. Boulton,

Eds., Academic Press, New York, NY, Vol. 25, p- 83 and N. K.

Ushenko and T. E. Gorizdra, Ukrain Khim. 2Zhur., 16, 545 (1950).

An alternate procedure for the preparation of the above compound when Y¥ =O is the preparation of the corresponding oxazole 4 followed by oxidation/hydrolysis to the desired oxazole I wherein X is O, X, is NH and Y is O (Scheme 2):

Scheme 2

Q 0

NC —_— SN o—& o—&

S 0 4 I wherein X is O,

X, is NH, and

Y is O.

\ | 07 -15-

For the preparation of 4, see, for example, F. C. Brown,

Chem. Rev., 61, 463 (1961), and the (Newkome) reference previously cited. For the conversion 4 to I of Scheme 2, see also J. W. Clark-Lewis, Chem. Rev., 58, 63 (1958), and N. A.

Shenberg, L. S. Guseva, A. I. Ginak, and E. G. Sochilin,

Zhur. Organ. Khim., 14, 1323 (1978).

Variations of the above Scheme 1 are also readily prepared by the methods of Scheme 1' discussed above. These are shown here in Scheme 1" as follows.

Scheme 1" 0 0

HOAC

: ArCHO + NH ——= ar’ XX “nn

NaOAc =, —K, and 0 0

HOAC

ArCHO + NH ———= ar’ NH { NaOAc {

S S s A s

An alternate procedure for the preparation of the above noted oxazole I wherein X is 0, X; is NH, and Y is 0 is by an alkylation/hydrolysis procedure (Scheme 2') as follows:

“ yo” ~-16-

Scheme 2' > 0 ne SNC _EEaN | SNC | Et NH Jor o—4 04,

S S

® 0 0

NC ov A AN a r NH Fo CH33H oN a, Ho KX,

The procedure of Scheme 2' is essentially that of

N. A. Shenberg, L. S. Guseva, A. I. Ginak, and

E. G. Sochilin, Zhur. Organ. Khim., 14, 1323 (1978); and J. S. H. Davies, W. Hook, and F. Long,

J. Chem. Soc., 30 (1950). .

The compounds of formula I are useful both in the free acid form, in the form of base salts where possible, and in the form of acid addition salts. The three forms are within the scope of the invention. In practice, use of the salt form amounts to use of the base form. Appropriate pharmaceutically acceptable salts within the scope of the invention are those derived from mineral acids such as hydrochloric acid and sulfuric acid; and organic acids such as methanesulfonic acid, benzenesulfonic acid, p-toluenesulfonic acid, and the like, giving the hydrochloride, sulfamate, methanesulfonate, benzenesulfonate, p-toluenesulfonate, and the like, respectively or those derived from bases such as suitable organic and inorganic bases. Examples of suitable inorganic bases for the formation of salts of compounds of this invention include the

17° - 1 7- hydroxides, carbonates, and bicarbonates of ammonia, sodium, lithium, potassium, calcium, magnesium, aluminum, zinc, and the like.

Salts may also be formed with suitable organic bases.

Bases suitable for the formation of pharmaceutically acceptable base addition salts with compounds of the present invention include organic bases which are nontoxic and strong enough to form such salts. These organic bases form a class whose limits are readily understood by those skilled in the art. Merely for purposes of illustration, the class may be said to include mono-, di-, and trialkylamines, such as methylamine, dimethylamine, and triethylamine; mono-, di-, or trihyd:-oxyalkylamines such as mono-, di-, and triethanolamine; amino acids such as arginine, and lysine; guanidine; N-methylglucosamine; N-methylpiperazine; morpholine; ethylenediamine: N-benzylphenethylamine;: tris(hydroxymethyl) aminomethane; and the like. (See for example, "Pharmaceutical Salts," J. Pharm. Sci., 66(1), 1-19 (1977).)

The acid addition salts of said basic compounds are prepared either by dissolving the free base of compound I in aqueous or aqueous alcohol solution or other suitable solvents containing the appropriate acid or base and isolating the salt by evaporating the solution, or be reacting the free base of compound I with an acid as well as reacting compound I hav.ng an acid group thereon with a base such that the reactions are in an organic solvent, in which case the salt separates i:rectly or can be obtained by concentration of the soi.z.2n.

The base salts of c:mpounds of formula I described above are prepared by reacting -ne appropriate base with a stoichiometric equivalen: >I the acid compounds of formula I to obtain pharmacological.y acceptable base salts thereof.

The acid solution sa.-s of said basic compounds are prepared either by dissolv:.:ng the free base in aqueous or aqueous alcohol solution zr other suitable solvents containing the appropriate acid and isolating the salt by ‘sh ORIGINAL J

’ 1 - 1 8 - evaporating the solution, or by reacting the free base and acid in an organic solvent, in which case the salt separates directly or can be obtained by concentration of the solution.

The compounds of this invention may also exist in hydrated or solvated forms.

The products of the reactions described herein are isolated by conventional means such as extraction, distillation, chromatography and the like.

The antiallergic and antiinflammatory activity of the compounds having the formula I of the present invention is determined by an assay showing inhibition of the release of histamine from human basophils (HHB). a description of the protocol of the HHB assay is found hereinafter.

Thus, pharmaceutical compositions are prepared from the compounds of formula I and salts thereof described as the present invention in unit dosage form comprising the compound either alone or in admixture with a pharmaceutically acceptable carrier appropriately selected from those known.

A physician or veterinarian of ordinary skill readily determines a subject who is exhibiting allergic or inflammatory symptoms. Regardless of the route of administration selected, the compounds of the present invention are formulated into pharmaceutically acceptable dosage forms by conventional methods known to the pharmaceutical art,

The compounds can be administered in such oral unit dosage forms such as tablets, capsules, pills, powders, or granules. They also may be administered rectally or vaginally in such forms as suppositories or bougies; they may also be introduced parenterally (e.g., subcutaneously, intravenously, or intramuscularly), using forms known ro the pharmaceutical art. They are also introduced directly to an affected area (e.g., in the form of eye drops or by inhalation). For the treatment of allergic or inflammatory conditions such as erythema, the compounds of the present invention may also be administered topically in the form of el”

J

~-19- ointments, creams, gels, or the like. In general, the preferred route of administration is orally.

An effective but nontoxic quantity of the compound I is employed in treatment. The ordinarily skilled physician or veterinarian will readily determine and prescribe the effective amount of the antiallergic or antiinflammatory agent to prevent or arrest the progress of the condition.

The dosage regimen is selected in accordance with a variety of factors including the type, age, weight, sex, and medical condition of the mammal, the severity of symptoms of the disease being treated, the route of administration and } particular compound of formula I employed. An ordinarily skilled physician or veterinarian will readily determine and prescribe the effective amount of the compound I to prevent or arrest the progress of the condition. In so proceeding, the physician or veterinarian could employ relatively low dosages at first, subsequently increasing the dose until a maximum response is obtained. For convenience, the total daily dosage may be divided and administered in portions during the day if desired. In so proceeding, the physician or veterinarian could employ relatively low dosages at first, subsequently increasing the dose until a maximum response is obtained.

Initial dosages of the compounds of the invention having formula I are ordinarily in the area of 10 mg up to 2 g per day orally, preferably 20 mg to S00 mg per dose orally, given from one to four times daily or as needed. When other forms of administration are employed, equivalent doses are administered.

A suitable dose of a compound of formula (I) or pharmacologically acceptable salt thereof for a mammal suffering from, or likely to suffer from any condition as described hereinbefore is 0.1 ug —- 500 mg of the compound per kilogram body weight. In the case of systemic administration, the dose may be in the range of 0.5 to 500 mg of the compound per kilogram body weight, the most preferred dosage being 0.5 to 50 mg/kg of mammal body weight administered two or

. 0

PE

~20-~ three times daily. In teh case of topical administration, e.g., to the skin or eye, a suitable dose may be in the range 0.1 ng-100 ug of the compound per kilogram, typically about 0.1 ug,/kg.

In the case of oral dosing for the treatment or prophylaxis of arthritis or inflammation in general, due to any course, a suitable dose of a compound of formula (I) or physiologica! 7 acceptable salt thereof, may be as specified in the preceding paragraph, but most preferably is from 1 mg to 10 mg of the compound per kilogram, the most preferred dosage being from 1 mg to 5S mg/kg of mammal body weight, for example, from 1 to 2 mg/kg.

It is understood that the compositions and methods of treatment of the present invention as described above also include the free acid, the pharmacologically acceptable base salts and acid addition salts of the compounds of formula I.

The following Examples further illustrate the invention, but are not meant to be limiting thereto.

EXAMPLE 1 5-[(2,3-Dimethoxyphenyl)methylene]-2-thioxo-4-thiazolidincne

A mixture of 2,3-dimethoxybenzaldehyde (5.2 g, 30 mmoles), rhodanine (4.0 g, 29 mmoles), sodium acetate (8.4 g, 102 mmoles), and acetic acid (50 ml) is stirred under an inert atmosphere and heated to reflux. After 4 hours the mixture is stirred into water (250 ml) and the precipitate is filtered off, rinsed successively with water (3X), ethanol (2X), and ether (2X), and dried to afford the pure product (7.8 g), mp 268-269°C.

TABLE 1

The following examples are prepared from the corresponding benzaldehydes using the procedure described in Example 1.

a : 04 -_ 2 1 -

Ry 0 s—&

Rj 5

Ry,

Example _R; _R, ~~ Ry _Rqy _ mp °C 2 OMe H OMe H 275-276 3 OMe H H OMe 245-246 4 OMe OMe OMe H 205-206 5 OMe H OMe OMe 297 (dec) 6 H OMe OMe OMe 200-201 7 cl H cl H 234-235 (Recryst. from DMF) 8 H Cl Cl H 241-242 9 H OMe OMe H 232-234 10 H OMe OH OMe 252-253 (Recryst. from MeOH) 11 H F OMe H 264-265 12 H OH OH H *0300 13 H Br OH H 283-285 (Recryst. from EtOH) 14 H Br OH Br >@300 15 H Me OH Me 293-295 16 H OMe OH Br 275 (dec) 17 H H H H 204-205 18 MeO H H H 249-250 19 H MeO H H 232-233 20 H H MeO H 250-251 21 H OH H H 246-248 22 H H OH H 288 (dec) 23 H OEt H H 170-172 24 H OCHMe, H H 191-193 25 H OCH, Ph H H 197-198 26 H Me H H 198-200 27 H CFq4 H H 189-190 28 H Cl H H 233-235 i . pY pol ov , —- 2 2-

TABLE 1 (cont'd)

Example _R, R, By Ry _mp °C 29 H Br H H 239-241 30 H NO, H H 265-267 31 H H Cl H 224-226 32 H H Br H 236-238 33 H H OPh H 150-151 34 H H NO, H 265-267 35 H H SMe H 258-260 36 H MeO OH H 229-230 37 H MeO H MeO 257-259 38 H H Me H 225-227 39 H H Ph H 240-242

EXAMPLE 40 5-[(2,3-Dichlorophenyl)methylene]-2-thioxo-4-thiazolidinone

A mixture of 2,3-dichlorobenzaldehyde (4.6 g, 26 mmoles), rhodanine (3.5 g, 25 mmoles), sodium acetate (7.5 g, 91 mmoles), and acetic acid (40 ml) is stirred under an inert atmosphere and heated to reflux. After 2.5 hours the mixture is stirred into ice-water (250 ml) and the precipitate is filtered off, rinsed three times with water and dried. Recrystallizar:zn from methanol gave the pure product (2.9 g), mp 203-224°C.

The following examp.es are prepared from the corresponding benzaldehyes .sing the procedure described in

Example 40: oS \ el ‘aAD ORIGINAL = \

Vi J 19" -23-~

Recryst.

Example Ry BR, Ry Ry Ry mp °C Solvent 41 CL H H H Cl 179-181 -—- 42 H tBu OH tBu H 248-249 EtOAc 43 H F H H H 201-202 MeCN 44 OH H H H H 216 (dec) MeCN 45 H OH OMe H H 224-225 MeOH 46 H iPr OH iPr H 200-202 MeCN 47 H OH OMe OMe H 212-213 MeOH 48 OMe H H H OMe 273-275 DMF 49 OH H H OMe H 253 (dec) -—- 50 H OH H OMe H 259-260 THF 51 H OMe OH tBu H 233-234 MeCN 52 OH H OMe OMe H 258 (dec)

EXAMPLE 53 5-([3-((Dimethylamino)methyl]-4-hydroxy-5- methoxyphenyl Jmethylene]-2-thioxo-4-thiazolidinone

A mixture of 3-[(dimethylamino)methyl]-4-hydroxy-5S- methoxybenzaldehyde (2.5 g, 12 mmoles), rhodanine (1.6 g, 12 mmoles), B-alanine (0.6 g, 7 mmoles), and acetic acid (35 ml) is stirred under an inert atmosphere and heated to reflux. After 4 hours the acetic acid is removed by rotary evaporator and the residue dissolved in water (250 ml). The solution is brought to approximate neutrality by addition of saturated aqueous sodium bicarbonate, and stirred for 3 hours. The precipitate is filtered off, rinsed three t mes with water and dried, then triturated in boiling methanol (100 ml), cooled, filtered off and dried to afford the pure product (2.9 g), mp 198°C (dec).

EXAMPLE 54 5-((5-Bromo-2-thienyl)methylene]-2-thioxo-d-thiazolidinone

Prepared by the method described in Example 1 from 5-bromo-2-thiophenecarboxaldehyde, mp 250-252° (dec).

INTERMEDIATE A

N,6-Dimethoxy-N-methyl-2-benzofurancarboxamide

To a stirred solution of 6.0 g (0.031 mole) of 6-methoxy-2-benzofurancarboxylic acid (A. McGookin, A.

Robertson, and W. B. Whalley, J. Chem. Soc. 787 (1940)) in 100 ml of tetrahydrofuran (under a nitrogen atmosphere) was added in one portion, 3.3 ml (4.8 g; 0.038 mole) of oxalyl chloride. The mixture was stirred for 5 minutes, then treated with 3 drops of N,N-dimethylformamide. After stirring at room temperature for 2 hours, the solvent was evaporated to leave a residue of crude 6-methoxy-2- penzofurancarbonyl chloride, mp 96-99° (a mp of 101° is given by A. McGookin, etc., cited above). The residue was re-dissolved in 100 ml of dichloromethane, and the solution was filtered. The filtrate was added dropwise over 20 minutes to an ice-cooled solution of 3.6 g (0.037 mole) of

N,0-dimethylhydroxylamine hydrochloride and 9.4 ml (7.7 g: 0.077 mole) of l-methylpiperidine in 100 ml of dichloromethane. After stirring for 2 hours, the mixture was added to 400 ml of ice-cold 5% aqueous hydrochloric acid.

The layers were separated, and the aqueous layer was extracted with fresh dichloromethane (3X 100 ml). The ccmbined organic layers were washed with water (1X 200 ml), 3% aqueous sodium bicarbonate (2X 200 ml), and water again.

The extracts were dried (anhydrous magnesium sulfate) and evaporated. Trituration of the residue with ether yielded 5.4 g (74% yield) of the analytically pure amide product, mp 73-77°.

Calcd. for C,H 3NO4: c, 61.27: H, 5.57; N, 5.96

Found: C, 61.26; H, 5.52; N, 5.78.

Ya —- 2 5-

INTERMEDIATE B

6-Methoxy-2-benzofurancarboxaldehyde

An ice-cooled solution of 4.0 g (0.017 mole) of

N,6-dimethoxy-N-methyl-2-benzofurancarboxamide in 100 ml of tetrahydrofuran was treated cautiously over 15 minutes (under a nitrogen atmosphere) with 0.65 g (0.017 mole) of lithium aluminum hydride. The mixture was stirred for 1 hour, then quenched by the careful addition of 100 ml of saturated aqueous sodium bisulfate solution. The reaction mixture was extracted with ether (3X 75 ml), and the combined extracts were washed with cold 2% aqueous hydrochloric acid (2X 100 ml), followed by brine (1X 100 ml). The organic layer was dried (anhydrous magnesium sulfate) and evaporated.

The residue was chromatographed (silica gel, dichloromethane elution) to yield 2.2 g (74% yield) of the analytically pure aldehyde, mp 75-77°.

Calcd. for C;gHa04:

C, 68.18; H, 4.58

Found: CC, 68.03; H, 4.48.

EXAMPLE 55 5-{(6-Methoxy-2-benzofuranyl)methylene]-2-thioxo-4- thiazeclidinone

A mixture of 1.5 g (0.0085 mole) of 6-methoxy-2- benzofurancarboxaldehyde, 1.1 g (0.0083 mole) of 2-thioxo-4- thiazolidinone ("rhodanine"), and 2.5 g (0.030 mole) of anhydrous sodium acetate in 40 ml of glacial acetic acid {under a nitrogen atmosphere) was stirred and heated at reflux for 5 hours. The cooled mixture was added to 250 g of ice/water. The precipitated solid was filtered, washed with water, then with a little ccld ethanol to yield 2.0 g (81% yield) of the analytically pure thiazole, mp > 290°.

Calcd. for C 3HgNO,;S,:

Cc, 53.59; H, 3.11; N, 4.81: S, 22.01

Found: CC, 53.82; H, 3.08; N, 5.15; S, 21.71

~26~

The following Examples are for compounds also prepared by the procedures described the preparation of intermediates

A and B and Example 55 above.

EXAMPLE 56 5-((5-Methoxy-2-benzofuranyl)methylene]-2-thioxo-4- thiazolidinone mp 261-263° (recrystallized from acetonitrile/N,N- dimethylformamide).

Calcd. for C 3HgNO,S,: €C, 53.59; H, 3.11; N, 4.81; Ss, 22.01

Found: CC, 53.68; H, 3.23; N, 4.87; Ss, 21.88.

The starting carboxylic acid was S-methoxy-2- benzofurancarboxylic acid (W. B. Whalley, J. Chem. Soc., 3479 (1953)).

EXAMPLE 57 5-((Benzo(bJthien-2-yl)methylene]-2-thioxo-4-thiazolidinone mp 245°-dec (recrystallized from acetonitrile/N,N- dimethylformamide).

Calcd. for C,,H,NOS,:

C, 51.96; H, 2.54; N, 5.05: S, 34.68

Found: CC, 51.87; H, 2.41; N, 5.13; S, 34.45.

The starting carboxylic acid was commercially available benzo{b]thiophene-2-carboxylic acid.

EXAMPLE 58 5-[[5-Methoxy-3-(l-methylethoxy)benzo(b]thien-2- ylimethylene|-2~thioxo-4-thiazolidinone mp 242°-dec.

Calcd. for CieHgNO35,4:

C, 52.58; H, 4.14; N, 3.83; S, 26.32

Found: CC, 53.00; H, 4.13; M, 3.75; S, 25.95

0 -27-

The starting carboxylic acid was 3-(l-methylethoxy)-5- methoxy-benzo(bj]thiophene-2~-carboxylic acid (D. T. Connor,

W. A. Cetenko, P. C. Unangst, and E. A. Johnson, U.S. Patent

No. 4,703,053.

EXAMPLE 59 5-[(5-Methoxy-l-phenyl-1H-indol-2-yl)methylene)-2-thioxo-4- thiazolidinoine mp 250-253° (recrystallized from aqueous acetonitrile/N,N- dimethylformamide).

Calcd. for C gH; (N;0,S8, 1H,0:

C, 59.35; H, 4.19; N, 7.29; S, 16.68

Found: C, 59.56; H, 4.03; N, 7.06; S, 16.02,

The starting carboxylic acid was S-methoxy-l-phenyl-1H- indole-2-carboxylic acid (P. C. Unangst, D. T. Connor, S. R.

Ls Stabler, and R. J. Weikert, J. Heterocyclic Chem., 24, 811 (1987)).

EXAMPLE 60 5-[ (S-Methoxy-1-phenyl-1H-:ndol-2-yl)methylene]-2-thioxo-4- thiazolidinone, l-methylpiperidine salt

A suspension of 3.0 g (0.0082 mole) of S5-[(5-methoxy-1- phenyl-1H-indol-2-yl)methy.ere|-2-thioxo-4-thiazolidinone in 50 ml of methanol was treazed with 1.1 ml (0.89 g; 0.0090 mole) of l-methylpiper:.dine. The mixture was stirred briefly on the steam bath until homogeneous and filtered warm. Cooling to room temperature overnight yielded 3.2 g (84% yield) of the crystal..ne, analytically pure salt, mp 188-1917,

Calcd. for C gH; 4Ny0,S, C4 HN:

C, 64.48; H, 5.3%; N, 9.03; S, 13.77

Found: C, 64.02; H, 5.31: N, 8.82; S, 13.42.

J ORIGINAL J J

. He . ¥ -28-

EXAMPLE 61 5-((5-Bromo-2-thienyl)methylene]-2-thioxo-4-thiazolidinone, monsodium salt

A suspension of 2.0 g (0.0065 mole) of 5-[(S-bromo-2- thienyl)methylene]-2-thioxo-4-thiazolidinone in 25 ml of ethanol was treated with 3.3 ml of 2.00 N aqueous sodium hydroxide solution. The mixture was warmed on the steam bath until homogeneous and filtered warm. Cooling yielded 1.2 g (56% yield) of the analytically pure salt, mp 288°-dec.

Calcd. for CgH4BrNOS,Na-1.5 HO:

Cc, 27.05; H, 1.70; N, 3.94

Found: C, 27.06; H, 1.66; N, 3.70.

EXAMPLE 62 5-{ (3-Bromo-4-hydroxy-5-methoxyphenyl)methylene]-2-thioxo-4- thiazolidinone, disodium salt

A suspension of 3.46 g (0.010 mole) of S-((3-bromo-4- hydroxy-5-methoxyphenyl)methylene]-2-thioxo-d-thiazolidinone in SO ml of water was treated with 10.0 ml of 2.00 N aqueous sodium hydroxide solution. The mixture was warmed on the steam bath until nearly homogenous and filtered warm. The cooled filtrate was subjected to vacuum freeze-drying to leave a residue of 3.8 of red solid. The solid was digested briefly on the steam bath with 200 ml of acetone. Filtration gave 3.5 g (88% yield) of the analytically pure salt, mp 310°.

Calcd. for C, HgBrNO,;S,Na, 0.5 Hy0:

Cc, 33.09; #, 1.77; N, 3.51

Found: C, 33.03; H, 1.73; N, 3.36.

- VA -29-

EXAMPLE 63 s-( (4-Hydroxy-1,5-dimethoxyphenyl)methylene]=2.4- thiazolidinedione

Prepared according to the procedure described in Example 1 using 3,5-dimethoxy-4-hydroxybenzaldehyde (4.8 9g. 26 mmoles) and 2,4-thiazolidinedione (2.9 g, 25 mmoles).

Recrystallization from methanol gave the pure product (1.7 g), mp 248-249°C.

EXAMPLE 064 o-((3,5-Bis(L,l-dimethylethyl)-4-hydroxyphenyl|methylene]- 2,4-thiazolidinedione

A mixture of 3. 5-di-t-butyl-d4-hydroxybenzaldehyde (6.5 g, 27 mmoles), 2,4-thiazolidinedione (3.0 g, 26 mmoles), sodium acetate (7.6 g. 93 mmoles), and acetic acid (40 ml) is stirred under an inert atmosphere and heated to reflux.

After 78 hours the mixture is stirred into water (300 ml), and the precipitate is filtered off, rinsed three times with water, dried, and recrystallized from ethanol. The product is stirred in 1 N NaOH (50 ml) and extracted several times with dichloromethane. The aqueous solution is acidified with acetic acid, stirred for 1-2 hours, and the precipitate is filtered off, rinsed three times with water and dried to afford the pure product (2.3 g), mp 238-240°C.

The following compounds are prepared by the method described in Example 64: 2 0 4 6 s—& 5 0

] Nf ~-30-

Example R BN MP 65 H 247-249 66 3-OMe 197-198 67 4-OMe 218-220 68 3-OMe, 4-OH 225-227

INTERMEDIATE C

2-Thioxo-4-oxazolidinone

A solution of 72.9 g (0.75 mole) of potassium thiocyanate and 48.9 g (0.75 mole) of potassium cyanide in 85 ml of water was stirred in an ice bath and treated over 30 minutes with 60 ml (~0.80 mole) of 37% aqueous formaldehyde solution. The rate of addition was adjusted to maintain a reaction temperature of <10°. The mixture was stirred for an additional 90 minutes, then treated over 1 hour with 156 ml of concentrated hydrochloric acid (reaction temperature maintained at 10°). The mixture was stirred for 16 hours as it slowly warmed to room temperature.

The inorganic solids were filtered and discarded, and the filtrate was warmed on the steam bath for 90 minutes. The reaction mixture was again filtered, and the filtrate was extracted with ether (5X 200 ml). The combined extracts were dried (anhydrous sodium sulfate) and evaporated to an oil, which slowly crystallized. The solid was washed with hexane to yield 22.7 g (26% yield), mp 106-109°. A sample recrystallized from ethyl acetate/hexane was analytically pure, mp 110-112° (a mp of 113° is given by N. K. Ushenko and

T. E. Gorizdra, Ukrain. Khim. Zhur., 16, 545 (1950)).

EXAMPLE 69 5-( (4-Hydroxy-3-methoxyphenyl)methylene]-2-thioxoz4- oxazolidinone

A mixture of 3.25 g (0.021 mole) of 4-hydroxy-3- methoxybenzaldehyde, 2.34 g (0.020 mole) of 2-thioxo-4-

. A

M

-31- oxazolidinone, 5.8 g (0.071 mole) of sodium acetate and 15 ml of acetic acid was stirred and heated at reflux under a nitrogen atmosphere for 2 hours. The mixture was ther stirred at room temperature for 16 hours, and added to 300 g

S of ice/water. The precipitated solid was filtered, washed with water, and recrystallized from aqueous methanol /N,N- . dimethylformamide to yield 2.4 g (49% yield) of the oxazole product. A sample recrystallized a second time as above was analytically pure, mp 240°-dec. (A mp of 237-238° is given by rT. E. Gorizdra and S. N. Baranov, Zhur. Obshchei Khim., 26. 3092 (1956)). calcd. for C, HgNO4S 0.25H,0:

Cc, 51.65; H, 3.74; Ny 5.48; S, 12.54

Found: C, 51.54: H, 3.97: N, 5.38; S, 12.17

Also prepared by the procedure described in Example 69, utilizing the appropriate aldehyde intermediate were:

EXAMPLE 70 5-{ (3-Methoxyphenyl)methylene]-2-thioxo-d-oxazolidinone mp 215°-dec (recrystallized from methanol (N,N- dimethylformamide). ] calcd. for C ,HgNO;3S: c, 56.15; H, 3.86; N. 5.95; S, 13.63

Found: C, 56.34: Hy 3.87; N, 5.95: S, 13.51.

EXAMPLE 71 5-( (4-Methoxyphenyl methylene] =2-thioxo-4-oxazolidinons mp 205-207° (recrystallized from aqueous methanol /N,N- dimethylformamide). (A mp of 192° is given by N. K. Ushenko and T. E. Gorizdra, Ukrain Khim. Zhur.. 16, 545 (1950)). calcd. for C,;HqNO3S: c, 56.15; H, 3.86; N, 5.95; S, 13.63

Found: C, 56.10; Hy 3.88; N, 6.03; S, 13.62.

ar) og iy ~-32-

EXAMPLE 72 5-((3,4-Dimethoxyphenyl)methylene]-2-thioxo-4-oxazolidinone mp 249°-dec.

Calcd. for C,H NO,S:

S C, 54.32; H, 4.18; N, 5.28; SS, 12.09

Found: Cc, 53.92; H, 4.10; N, 5.22; S, 11.84.

EXAMPLE 73 5-( (4-Hydroxy-3,5-dimethoxyphenyl)methylene}-2-thioxo-4- oxazolidinone mp 250°-~dec. (recrystallized from aqueous acetonitrile/N,N- dimethylformamide).

Calcd. for CoH NOS:

Cc, 51.24; H, 3.94; HN, 4.98; S, 11.40

Found: C, 51.00; H, 3.78: N, 5.30; S, 11.45.

EXAMPLE 74 5-{ (4-Hydroxy-3,5-dimethylptenyl)methylene]-2-thioxo-4- oxazolidinone : : mp 253°-dec.

Calcd. for CoH NO;S:

C, 57.81; H, 4.45: MN, 5.62; S, 12.86

Found: Cc, 57.84; H, 4.49; N, 5.56; S, 12.54.

TXAMPLE 75 5-( (3-Bromo-4-hydroxy-5-mezrcxyphenyl)methylene]-2-thioxo-4- oxazolidinone mp 260-262°

Calcd. for C, HgBrNO,S:

Cc, 40.01; H, 2.44: N, 1.24; S, 9.71: Br, 24.20

Found: C, 40.18; H, 2.34: N, 4.07; S, 9.61; Br, 24.02.

Al il ~33-

EXAMPLE 76 2-Thioxo=5~ ((2,4,5-crimetho xyphenyl)methylenel=d= oxazclidinone mp 265° -dec. (recrystallized from aqueous methanol /N, N= dimethylformamide).

Calcd. for CH gNOsS: c, 52.87; Hy 4.44; N, 4.74; Ss, 10.86

Found: CC, 52.55; H, 4.30; N, 4.79; gs, 10.47.

EXAMPLE 77 thioxo-5-1(34,5-trinethoxyphenyl methylene] t= oxazolidinone mp 230°-dec. (recrystallized from aqueous methanol /N,N- dimethylformamide).

Calcd. for Cy 3H aNOsS: c, 52.87; H. 4.44; N, 4.74; S, 10.86

Found: C, 52.81; H, 4.32; N, 4.62: S, 10.39.

EXAMPLE 78 _( (4-iydroxy-1, 5-dinethoxyphenyl methylene | 2s Ehioxe=S- oxazolidinone, disodium salt

A suspension of 3.1 g (0.011 mole) of 5-( (4-hydroxy-3,3"~

J imetnoxyphenyl methylene | -2-thioxo-4-oxazolidinone in 20 ml of water was treated with a solution of 11.0 ml of 2.00 N aqueous sodium hydroxide solution. The mixture was warmed briefly on the steam bath until nearly homogenous, then filtered warm. The cooled filtrate was subjected to vacuum freeze-drying to yield 3.5 9g (98% yield) of the analytically pure salt product, mp >265°.

Calcd. for C,,HgNOsSNa, 0.6 H,0:

Cc, 42.89; H, 3.06; N, 4.17

Found: GC, 42.67; H, 2.88; N, 4.00.

. L.A

AT

. - -34-

Also prepared by the above procedures was:

EXAMPLE 79 s( (4-Hydroxy-3-methoxyphenyl)methylene]=2-thioxo=d- oxazolidinone, disodium salt mp »270° calcd. for C H,NO4SNaj-1 HpO: c, 42.18; H, 2.90; N, 4.47

Found: CC, 41.84; H, 2.52; N, 4.37.

EXAMPLE 80 5-((3,5-Dibromo-4-hydroxyphenyl)methylene]-2-thioxo-4- imidazolidinone

Prepared according to the procedure described in Example 1 using 3,5-dibromo-4-hydroxybenzaldehyde (4.5 g, 16 mmoles), 2-thiohydantoin (1.7 g, 15 mmoles), sodium acetate (4.5 g. 55 mmoles), and acetic acid (35 ml). Recrystallization from ethanol gave the pure product (1.4 g), mp 267°C (dec).

EXAMPLE 81. 5-((4-hydroxy-3,5-bis(L-methylethyl)phenylimethylene] 2- thioxo-4-imidazolidinone

Prepared according to the procedure described in Example 1 using 4-hydroxy-3,5-bis(l-methylethyl)benzaldenyde (3.0 g, 15 mmoles). 2-thiohydantoin (1.6 g., 14 mmoles), sodium acetate (4.2 g, 51 mmoles), and acetic acid (35 ml), filtered off, dried, and recrystallized from acetonitrile to afford the pure product (1.4 g), mp 225-230°C.

or + - 3 5 —-

EXAMPLE 82 5-((3-Bromo-d4-hydroxyphenyl)methylene]-2-thioxo=-4- imidazolidinone

Prepared according to the procedure described in Example 1 using 3-bromo-4-hydroxybenzaldehyde (3.1 g, 15 mmoles), 2-thiohydantoin (1.7 g, 15 mmoles), sodium acetate (4.5 g, 55 mmoles), and acetic acid (35 ml), to afford the pure product (3.4 g), mp 262°C (dec).

EXAMPLE 83 5-([3,5-Bis(l,l-dimethylethyl)-4-hydroxyphenyl|methylene]-2- thioxo-4-imidazolidinone

Prepared according to the procedure described in Example 1 using 3,4-di-t-butyl-4-hydroxybenzaldehyde (6.2 g, 26 mmoles), 2-thiohydantoin (2.9 g, 25 mmoles), sodium acetate (7.5 g, 91 mmoles), and acetic acid (40 ml).

Heating under reflux is maintained for 36 hours.

Recrystallization from acetonitrile gave the pure product (4.0 g), mp 279-281°C (dec).

EXAMPLE 84 5-( (2-Methoxyphenyl)methylene]-2-thioxo-4~-imidazolidinone

A mixture of 2-methoxybenzaldehyde (2.5 g, 18 mmoles), 2-thiohydantoin (2.0 g, 17 mmoles), sodium acetate (4.0 g, 49 mmoles), acetic anhydride (4 ml), and acetic acid (15 ml) is stirred under an inert atmosphere and heated to reflux.

After 1 hour the mixture is stirred into water (300 ml), and the precipitate is filtered off, rinsed with water three times and dried. The product is triturated in boiling methanol, filtered off, and dried to afford the pure product (3.4 g), mp 234-235°C.

- pt! —- 3 6 -

The following examples are prepared from the corresponding benzaldehydes using the procedure described in

Example 84.

Q

HE

Rj S

Rj

Recryst.

Example _Ry Rp _Ry mp °C Solvent 85 OMe H H 238-239 MeOH 86 H OMe H 266-267 MeOH 87 OMe OH H 237-238 MeCN 88 OMe OH OMe 268 (dec) MeOH

EXAMPLE 89 6 ((4-(Acetyloxy)-3-methoxyphenyl]methylene]=2.4- imidazolidinedione prepared according to the procedure described in Example 84, using 4-acetoxy-3-methoxybenzaldehyde (4.0 g, 21 mmoles) and hydantoin (2.0 g, 20 mmoles). Recrystallization from methanol/DMF gave the product (1.8 9g), MP 275-277°C, retaining 0.15 equivalents of DMF.

EXAMPLE 90 5-( (4-Hydroxy-3-methoxyphenyl)methylene]=2,4- imidazolidinedione

A mixture of 4~hydroxy-3-methoxybenzaldehyde (4.6 g, mmoles), hydantoin (3.0 g, 30 mmoles), B-alanine (1.4 ag,

16 mmoles), and acetic acid (40 ml) ig stirred under an inert atmosphere and heated to reflux. After 4 hours the mixture is stirred into water (350 ml) and the precipitate is filtered off, rinsed successively with water (3X). ethanol (2X), and ether (2x), and dried. Recrystallization Erom methanol afforded the pure product (3.0 g), mp 272-273°C.

EXAMPLE 91 5-[(3, 5-pimethoxy-d4-hydroxyphenyljmethylene 1-2,4- imidazolidinedione

Prepared according to the procedure described in Example 90 using 3,5-dimethoxy-d4-hydroxybenzaldehyde (5.6 9. 30 mmoles), hydantoin (3.0 g, 30 mmoles), and p-alanine (1.4 g, 16 mmoles), to afford the pure product (6.0 g), mp 296-297°C.

EXAMPLE 92 o-((3,5-Bis(1,l-dimethyletnyl)-d-hydroxyphenyl methylene ls 2,4-imidazolidinedione

A mixture of J. 5-di-t-butyl-4-hydroxybenzaldehyde (7.0 g, 30 mmoles), hydantoin (3.0 g, 30 mmoles), B-alanine (1.4 g, 16 mmoles), and ace-ic acid (40 ml) is stirred under an inert atmosphere and hea-ed to reflux. After 48 hours the mixture is stirred into water (300 ml). and the precipitate is filtered off, rinsed witn water three times, and dried.

Recrystallization from ace-onictrile gave the pure product

Js 25 (4.8 g), mp 251-252°C.

CX AMPLE 93 5 ((2-Methoxyphenyl)methylenel-3-methyl=2,4- imidazolidinedione

A mixture of J-methoxyoenzaldehyde (3.4 g,25 mmoles), 3-methyl hydantoin (3.0 g, 21 mmoles), sodium acetate (6.0 g.

- vr -38- 73 mmoles), acetic anhydride (3 ml), and acetic acid (20 ml) is stirred under an inert atmosphere and heated to reflux. After 15 hours the mixture is poured into water (200 ml). stirred, and the precipitate filtered off, rinsed three times with water and dried. Recrystallization from acetonitrile gave the pure product (3.1 g), Pp 189-190°C.

EXAMPLE 94 5_[ (1-Methoxyphenyl)methylene]-3-methyl-2,4- imidazolidinedione prepared according to the procedure described in Example 93 using j-methoxybenzaldehyde (3.5 ml, 28 mmoles) and 3-methyl hydantoin {3.0 g, 21 mmoles), to afford the product (3.6 g), mp 203-205°C.

EXAMPLE 95 5 ( (4-Methoxyphenyl)methylene]-3-methyl-2,4- imidazolidinedione prepared according to the procedure described in Examp.e 93 using 4-methoxybenzaldehyde (3.4 g, 25 mmoles) and 3-methyl hydantoin (3.0 g, 21 mmoles). Recrystallization from acetonitrile gave the pure product (2.0 g), mp 221-222°C.

EXAMPLE 96 s-( (4-Hydroxy-3-methoxyphenyl)methylene]-3-mechyl=2.2- imidazolidinedione prepared according to the procedure described in Examp.e ' 93 using 4-hydroxy-3-methoxybenzaldehyde (3.2 gq, 21 mmoles) and 3-methyl hydantoin (3.0 g, 21 mmoles). Recrystallization from ethyl acetate gave the pure product (0.7 9), Mp 227-228°C.

. NI

NT

-39-

EXAMPLE 97 ((3,5-Dimethoxy-i-hydroxyphenyl)metnylene | -3netnylzt. A= imidazolidinedicne

A mixture of 3 5-dimethoxy-4-hydroxybenzaldehyde (3.2 a9. 18 mmoles). 3-methyl hydantoin {2.0 g, 18 mmoles), sodium acetate (6.0 g» 73 mmoles), acetic anhydride (3 ml), and acetic acid (25 ml) is stirred under an inert atmosphere and heated to reflux. After 72 hours the mixture is stirred into water (300 ml) and the precipitate is filtered off, rinsed three times with water and dried to give a product (4.0 9) which is a mixture of the desired product and its O-acetate.

A mixture of this product (2.1 9). sodium carbonate (3.5 9)

DMSO (5 ml). and methanol (50 ml) is stirred and heated to reflux. After 18 hours the mixture ig stirred into icewater (400 ml) and acidified with 4N HCl. The precipitate is filtered off, rinsed successively with water (3X), ethanol (2X), and ether (2X), and dried. Recrystallization from methanol/DMF gave the pure product (0.6 g), MP 269-270°C.

EXAMPLE 98 / 20 -( (3-Hethoxyphenyl methylene |= -nethyL=2,4- imidazolidinedione

A mixture of 5 -methoxybenzaldehyde (3.8 gq, 28 mmoles). 1-methyl hydantoin (3.0 g 26 mmoles), sodium acetate (8.4 Gg. 102 mmoles), acetic anhydride (10 ml), and acetic acid (40 ml) is stirred under an inert atmosphere and heated to reflux. After 24 hours the mixture is stirred into water (350 ml), stirred, and the resulting gum is isolated and washed by decantation, then crystallized from isopropanol.

Recrystallization from acetonitrile gave the pure product (1.1 g), OP 195-197°C.

“ 5 | ’ 7 ~-40~

EXAMPLE 99 (\-syaroxy-2-nethoxypneny mechy Lene |e ERLE imidazolidinedione aA mixture of s-nydroxy-3-methoxybenzaldenyde (1.6 9g. 30 mmoles) 1-methyl nydantoin (3.5 gv 30 mmoles), j-alanine (1.4 9. 16 mmoles). and acetic acid (40 ml) ig stirred under an inert atmosphere and heated to reflux. After 8 hours the mixture is stirred into water (300 ml) and the precipitate filtered off, rinsed successively with water (3X) ethanol (2X) and ether (2X), and dried. Recrystallization from acetonitrile gave the pure product (1.1 g}), @mP 197-198°C.

EXAMPLE 100 13. 5-pinethoxy-4-hydroxypnenyl methylene | SLES 5, 4-imidazolidinedione prepared according to the procedure described in Example 99 using | 5-aimethoxy-4-hydroxybenzaldenyce (5.8 g, 31 mmoles) and l-methyl hydantoin (3.5 gr 10 mmoles).

Recrystallization from ethanol gave the pure product (2.8 g).,MP 194-196°C ’

EXAMPLE 101 1s s-piprono-4-hydroxyphenyl methylene A REESE imidazolidinedione prepared according to the procedure described in Example 99 using , 5-aibromo-4-hydroxybenzaldenyas (B.4 9g. 30 mmoles) and 1-methyl hydantoin (3.5 9. 30 mmoles) to afford the pure product (8.2 g)r MP 271-277°C.

—d 1-

EXAMPLE 102

Lo ydgoxy-dss-bis(i-nethyletnylipnenyl neEnyiene lS methyl-2,4-imidazolidinedion® prepared according to the procedure described in Example 92 using \ s-aiisopropyl-d-hydroxybenzaldenyae (2.7 9. 13 mmoles) and l-methyl hydantoin (1.4 g, 12 mmoles).

Recrystallization from acetonitrile gave the pure product (0.4 g), OP 197-208°C.

The following compounds are prepared according to the procedure described in Example 1 using appropriate / corresponding starting materials.

EXAMPLE 103 1 La-pyridylymethylene|-2thioxoTERIAZOLIAITENE: mp >300°.

EXAMPLE 104 (2 Tnienyl methylene] -2-thioxomdTERIazOLITIIENES mp 227-229°. :

EXAMPLE 105 is ibcon2-Euranyl)metny enero ERixOm4ERIATONIERATES mp 201-202°.

EXAMPLE 106 { (1-phenyl-1a- indol-2-y1 methylene] “2TERIOOTE thiazolidinone, MP 282-283°.

a ~-42-

EXAMPLE 107 + scomon-nyaroxypnenyymeshy ene ENE oxazolidinoné, mp 240° (dec). prepared according to the procedure described in Example 69.

EXAMPLE 108 apt (hs dimes ions SRS thioxo-4-0xazolidinons

A mixture of 14.1 3 (0.060 mole) of 3, 5-di-tert-butyl=4- nydroxybenzaldenyde 7.0 g (0.060 mole) of 7-thioxo-4- oxazolidinone, 17.4 4 (0.21 mole) of sodium acetate, and 75 ml of acetic acid is stirred and heated at reflux under 2 nitrogen atmosphere for 20 hours. The cooled reaction mixture is added to 900 3 of ice/water and the precipitated product filtered and washed with water. There is obtained 16.2 9 (81% yield) of the oxazole product, suitable for further reaction.

A sample of the above crude product is chromatographed over silica gel, using elution with 2.5% ethyl acetate in dichloromethane followed by 25% ethyl acetate. The chromatography product is recrystallized from agueous acetonitrile to yield the analytically pure oxazole, mp 240°C dec. calcd. for C,gHz2aNO3S!

C, 64.83; Hy 6.95: 1.20

Found: c, 65.00; H, 6.95: MN 1.17.

EXAMPLE 109

SEE oxazolidinedione p solution of 10.0 3 (n.030 mole) of 5-((3,5-bis(l/ 1” een mentors Er dinone in 150 ml of tetrahydrofuran is cooled in ice and treated with 4.2 ml (3.0 g- 0.030 mole) of ¢riethylamine. aap ORIGINAL dD rt -43-

The mixture is stirred with ice cooling for one hour, then treated with 10.0 ml (22.8 0.16 mole) of jodomethane. The ice bath is removed and the mixture stirred for an additional / 24 hours. The reaction mixture is filtered and the filter cake is washed several times with fresh retrahydrofuran. The combined filtrates are evaporated and the residue is chromatographed (silica gel, 2% methanol in dichloromethane elution) to yield 4.4 9 (42% yield) of the purified 2-methylthio- intermediate. This material 1s hydrolyzed without further purification.

A solution of 3.0 g (0.086 mole) of the above )-methylthio-intermediate in 90 ml of ethanol is diluted with 30 ml of water and treated dropwise over 10 minutes with 5.0 ml of concentrated hydrochloric acid. The reaction mixture is stirred for 24 hours and the precipitated product ig filtered and washed with hexane. The crude yield is 1.44 g (53%). A sample recrystallized from ethyl acetate/hexane yielded the analytically pure oxazolidinedione, mp 239°C dec.

Calcd. for C,gHzaNO4: c, 68.12; Hy, 7.31; N, 4.41

Found: C. 68.22; H, 7.29; N, 4.05.

Also prepared by the above procedure, utilizing the appropriate 5-thioxo-4-oxazolidinone intermediate are:

EXAMPLE 110 { a-syaroxy-33-dinethoxypheny methylene 1-2, d-ORE2ELL SEES dione mp 268° dec. (recrystallized from acetonitrile/DMF/water).

Calcd. for C,H NOs: c, 54.34; H, 4.18; N, 5.28

Found: Ci 54.00; H, 4.17; N, 5.15.

. Pal

Co CA -44-

EXAMPLE 111 5-({4-Hydrox -3,5-dimethylphen 1)meth lene|-2,4-oxazolidine- dione mp 275°C dec. (recrystallized f rom acetonitrile/DMF/water). calcd. for CypH NO 0.5H,0¢ c, 59.50; H, 4.99: N, 5.78 found: C, 59.31: H, 5.02; N, 5.72. . EXAMPLE 112 5-(13,5-Bis(],1-dimathylethyl)-4-hydroxyphenyllnethyl® } ene ]-2-thioxo-4-oxazolidinone, choline salt. . \

A solution of 2.45 g (0.0069 mole) of 46.6% aqueous choline bicarbonate in 50 ml of methanol is stirred and treated over 5 minutes with 2.31 g (0.0069 mola) of §-[(3,5-Bis(1,-dimethylethyl)-4-hydroxy= phenyl ]mathylene]-2-thioxo-4-oxazollidlnona. The : reaction mixture ia warmed for a few minutes to reflux on the steam bath, and filtered hot. The cooled filtrate is avaporated, and the rasidue recrystallized from acetona/tert-putyl methyl ether to yield 2.1 ¢ (70% yield) of the analyticallly pure choline salt, mp 167° dec.

Calc. for C,yH76N20458 10 : Cc, 63.27; H, 8.31 N, 6.42

Found: Cc, 63.223 WU, g.21y N, 6.28 re ————— —— ~ 7 . 44A

EXAMPLE 113 : 5-{13,5-Bis(1,1-dimethylethyl)-4-hydroxyphenyl|methyl- ene)-2,4-thiazolidinedione, choline salt.

A solution of 46.6% aqueous choline bicarbonate (10.55 9g, 23.8 mmoles) is added dropwise under nitrogen to a stirred suspension of 5-1{3,5-Bis{l,1- dimethylethyl)-4-hydroxyphenyl]lmethylene]-2,4-thiazo- lidinedione (10.0 g, 30.0 mmoles) in ethanol (100 mls). The solution is gradually warmed to reflux for * one hour, and is then cooled and stripped of solvent by rotary evaporator. Two 50 ml portions of ethanol are successively mixed with and stripped from the residue which is then thoroughly dried under vacuum.

TrF2 residue is triturated in diethyl ether, filtered off, washed twice with ether, and dried under vacuun at 80°C overnight to afford the crystalline choline salt (12.8 g), mp 215°C dec.

Calc. for Cy gilyaN03S-Cslly 4 NO:

Cc, 63.27; u, 8.313 N, 6.42 : Found: Cc, 63.12; H, 8.28; N, 6.17 yo! 448 {- . : . The usefulness of the compounds of tha present ; invention as inhibitors of histamine release is demonatrated by the following assay. The assay is , easantially as generally accepted among the ordinarily skilled artisans to show activity having usefulness to treat the diseases or conditions as set out in the present invention. A description of the procedure follows, i

HISTAMINE RELEASE FROM HUMAN BASOPUILS

(hereinafter HHB) . : )

The HHB assay quantitates active histamine . release, and its inhibition by drugs, from basophils of human blood. Thus, the assay provides evaluation of the compounds of formula I for treating the conditions or diseases as is the present invention.

As descrioed herein the assay includes modifications of the method described by R. P. Siraganian in "An

Automated Continuous-Flow System foc the Extraction ‘ and Fluorometric Analysis of tilstamine®™, Anal. giochem., 57, 383-394 (1974). | :

METHODS :

Preparation of Leukocytes

Blood is drawn from allergic donors (chosen on the basis of adequate histamine release induced by a challenge), using standard vanipunctuce methods, into vVacutainers with EDFA in water as anti- coagulant. The blood samples are placed briefly

CY ki I". -495- on a rotary mixer. The blood is mixed with Hespan (hydroxy ethyl starch, 0.5 ml per 1.0 ml of blood), the tube inverted several times to mix and then left undisturbed at room temperature until a sharp separation is observed between the settled red cells and the leukocyte and platelet-rich plasma.

This usually occurs within 35-45 minutes.

The plasma fraction is removed and centrifuged for 12 minutes at 4°C at 1050 RPM (100 Xg). The platelets remain in the plasma and are discarded. The pelleted leukocytes are shaken gently to disrupt the cell button and washed twice with HA buffer containing 0.005 M EDTA and resuspended in

HACM buffer to approximately one-quarter the original blood volume. A sample is prepared for Hematology, where a total white blood cell and platelet count is done using a Coulter

Counter. protocol Design

Aliquots (0.1 ml) of cells are added to triplicate assay tubes containing 0.4 ml of either 6% perchloric acid (for total histamine content), vehicle control (for spontaneous release), OL drug. The tubes are incubated at room temperature for 8 minutes, and then placed in a 37°C water bath for 2 more minutes. Buffer or challenge agents (at 37°C) are added to the tubes and they are incubated for an additional 45 minutes at 37°C in a shaking water bath. The tubes are then spun at 2000 RPM (1200 g) for 3 minutes to pellet the cells and the supernatants are removed and assayed for histamine by the fluorometric method.

Drug Preparation

A 300 uM stock solution of each rest compound is prepared in distilled water, using 0.5 ml DMSO/100 ml and/or 0.2 ml of LN NaOH or HCl and/or heat to aid in dissolution.

Five ml of the stock solution is diluted (1:2) with 5 ml of two times concentrated HACM buffer to yield the stock working concentration of 150 uM. When added to the cells and 15 stimulus, a final test concentration of 100 pM drug results.

Further dilutions are made with HACM buffer for 33, 10. 3.3, 1.0 pM, etc.

- Lp : . Xi -46-

Challenge Agent Preparation

Short ragweed and house dust extracts (Greer

Laboratories, Inc.) are supplied as aqueous extracts ‘n stock concentrations of 40,000 and 10,000 protein nitrogen units per milliliter (PNU/ml), respectively. Aqueous solutions of anti-IgE antisera (rabbit-raised antibody) are purchased from

Dako via Accurate Chemicals. The aqueous solutions of ragweed, house dust, and anti-IgE are diluted 1:2 with two times concentrated HACM and then further diluted with HACM to yield final stock concentrations of 6000 PNU/ml for ragweed and house dust and 1:50 dilution for the anti-IgE antisera.

Further dilutions for working solutions are made in HACM buffer. All stock and working solutions are stored at 4°C.

Working solutions comprise 1/6 of the final volume in the cell reaction, therefore, working solutions of challenge agents are made up six times the required final concentration.

In each experiment, cells are challenged according to the previously determined sensitivity of that donor to the particular challenge agent. Short ragweed and house dust concentrations are expressed in pPNU/ml, and anti-IQE antisera is expressed as dilutions, e.g. IE-5 (1:100,000), 3E-S (1:30,000), and IE-4 (1:10,000).

Calculation and Interpretation of Results

The total histamine concentration in the "total" (acid-treated) samples must be 15 ng/ml to be acceptable.

Spontaneous release of histamine from the cells should not exceed 15% of the total histamine, and is frequently < 5%.

The maximum percentage histamine released varies with the donor. The net amount released by the challenge agent must exceed 25% of the total cellular histamine to confidently assess inhibition by test compounds. Spontaneous histamine release is subtracted from both "totals" and challenged cells to calculate net percent release. Percent inhibition is shown in the Table and is calculated using the following formula:

A

. Lath nl -47- 1-1 Mean net % release treated samples

Mean net % release for challenged control x 100 = % inhibition

; yo! yy -48-

TABLE

Ry 0 s—&

Rj Rg S

Ry

HHB

% Inhibition of Histamine Release

Ex Ry Rp Ra Re Bs at 33 uM

S 1 MeO MeO H H H * 2 MeO H OMe H H * 3 MeO H H OMe H 86 4 MeO OMe OMe H H *

MeO H OMe OMe H 73 6 H OMe OMe OMe H 92 7 Cl H cir H H 81 8 H Cl cl H H 88 9 H OMe OMe H H 84 10 H OMe OH OMe H 90 11 H F OMe H H 94 12 H OH OH H H 41 13 H Br OH H H 61 14 H Br OH Br H * 15 H Me OH Me H 84 16 H OMe OH Br H 51 17 H H H H H 39 18 MeO H H H H * 19 H MeO H H H 100 20 H H MeO H H 90

NI

-49-

HHB

3 Inhibition of Histamine Release

Ex R_ Rp Ra Rs Rg at 33 pM 21 H OH H H H 75 22 H H OH H H 95 23 H OEt H H H * 24 H OCHMe, H H H * 25 H OCH,Ph H H H * 26 H Me H H H 58 27 H CFj H H H 58 28 H Cl H H H * 29 H Br H H H * 30 H NO, H H H * 31 H H Cl H H 95 32 H H Br H H 61 33 H H OoPh H H 64 34 H H NO, H H * 35 H H SMe H H * 36 H MeO OH H H 79 37 H MeO H MeO H 80 38 H H Me H H 30 39 H H Ph H H : 28 40 C1 Cl H H H 60 41 Cl H H H Cl * 43 H F H H H * 44 OH H H H H *

on al -50~-

Rr, 0

OC s—&

Rj Rg S

Ry

HHB

$ Inhibition of Histamine Release

Ex BR. Ry Ra R4 Rs at 33 uM 45 H OH OMe H H 59 47 H OH OMe OMe H 79 48 OMe H H H OMe * 49 OH H H OMe H 98 50 H OH H OMe H 96 51 H OMe OH tBu H 34 52 OH H OMe OMe H 79 53 H Me,NCH, OH MeO H *

HHB

% Inhibition of Histamine Release

Ex Sat 33M

J 15 54 85 55 88

S56 : 26 57 ; 66 58 24 59 72 103 95 104 87 105 88 106 84

Ry 0

Ry ~ NH s—&

Rj Rs 0

Ry

HHB

% Inhibition of Histamine Release

Ex BR Ry Ra Ra Bs at 33 uM 63 H MeO OH MeO H 60 64 H tBu OH tBu H 49 65 H H H H H * 66 H OMe H H H 92 67 H H OMe H H 90 . 68 H OMe OH H H 81

Ry 0

R , Co . 2 vr S NH o—&

Rj Rs S ,

Ra

HHB

3 Inhibition of Histamine Release

Ex BR, Rp Ra Re Bs at 33 uM 69 H MeO OH H H 77 70 H MeO H H H 58 71 H H MeO H H 83 72 H MeO MeO H H 43 73 H MeO OH Meo H 89

Ai) . at ) bE -52-

HHB

3 Inhibition of Histamine Release

Ex Ry Ry Ra Ra Rs ar 33 uM 74 H Me OH Me H 88

S 75 H Br OH MeO H * 76 MeO H MeO MeO H * 77 H MeO MeO MeO H 76 107 H Br OH H H 66

Ry 0

R

? ~~ NH in —4

Rj Rg 5

Ry

HHB

3 Inhibition of Histamine Release

Ex Rj R, Ry Rg Rg at 33 uM 80 H Br OH Br H 85 82 H Br OH H H 78 83 H tBu OH tBu HH 30 84 OMe H H H H * 85 H OMe H H q 28 86 H H OMe H H * 87 H OMe OH H H * 88 H OMe OH OMe H 68 ra } ) T° ~53-

Ry 0

R2

NH im —&

Rj Rg 0

Rq

HHB

% Inhibition of Histamine Release

Ex BR, Ry; By Ry Bs at 33 uM 89 H MeO AcO H H 27 5S 90 H MeO OH H H * 91 H MeO OH MeO H * 92 H tBu OH tBu H 11

Ry 0

R . 2 x N= Me ian —&

Rj Rg 0

Rg

HH

4 Inhibition of Histamine Release

Ex Ry Ry Rg Ry Rs at 33wM 93 MeO H H H q * 94 H MeO H H H * 95 H H MeO H H * 36 H MeO OH H H 58 97 H Meo OH MeO H 51 yd pI / . -54-

Ry 0

OC

MH vet —&

R3 Rs 0

Ra

HHB

3 Inhibition of Histamine Release ex By Rez Ra Re Bs at 33 uM 98 H MeO H H H * 99 H MeO OH H H 92 100 H MeO OH MeO H 68 101 H Br OH Br H 96 + Indicates not active at 33 uM. g¢ Enhanced release at this concentration.

MeO is methoxy.

OCHMe, is isopropoxy.

OCH,Ph is benzyloxy. tBu is trertiarybutyl.

Me,NCH, is dimethylamine.

Activity is measured as inhibition of histamine release from human basophils challenged with anti-IgE at a 33 uM concentration of drug.

The ICgo iS calculated to give the pM necessary to provide 50% inhibition.

The usefulness of the compounds of the present invention as inhibitors of the 5-1lipoxygenase enzyme, cyclooxygenase, or in treating related diseases Of conditions may be demonstrated by their effectiveness in various standard test procedures. A description of each procedure follows.

a -55-

ARBL/ARBC Whole Cell S-Lipoxygenase and Cyclooxygenase Assays

Materials

The rat basophilic leukemia cell line (RBL-1) was obtained from the American Type Culture Collection (Rockville, MD).

Radioimmunoassay (RIA) kits of LTB, and PGF, were obtained from Amersham (Arlington Heights, IL) and Seragen (Boston, MA), respectively.

All tissue culture media were obtained from GIBCO (Grand

Island, NY).

Method

RBL-1 cells are grown in suspension culture in Eagle's minimum essential medium supplemented with 12% fetal bovine serum at 37°C in an incubator supplied with air-5% carbon dioxide. Cells are harvested by centrifugation. They are washed with cold phosphate buffered saline pH 7.4 (PBS; NaCl, 7.1 g; Na,HPO,, 1.15 g; KH,PO,, 0.2 g; and KCl, 0.2 g/l).

Cells are finally suspended in PBS containing 1.0 mM calcium at a density of 2 x 108 cells/ml. Cells are incubated with and without test agent (in DMSO) (1% DMSO is without effect on arachidonic acid metabolism) for ten minutes at room temperature. Calcium ionophore A23187 (5 uM) is added and cells are incubated for seven minutes at 37°C. The reaction is stopped by chilling the tubes on ice for ten minutes.

Cells are separated by centrifugation and the supernatant is stored at -20°. Aliquots (100 ul) are analyzed for LTB, and

PGF,, using radioimmunoassay kits as provided by the supplier.

Table 1 contains biochemical data obtained from this whole cell assay as ICgos which are calculated as the amount of test compound causing 50% inhibition of LTB, or PGFyq formation.

at 11 -56-

Carrageenan-Induced Rat Foot paw_Edema-2 (CEF-2) Assay: protocol

Carrageenan solution (1% w/v) is prepared by dissolving 100 mg carrageenan (Marine Colloidal Div., springfield. NJ) in 10 ml of sterile saline (0.9%) solution (Travenol). The solution is vortexed for 30 to 45 minutes. Animals are dosed with compound one hour before carrageenan challenge. Foot paw edema is induced by injecting 0.10 ml of the 1% carrageenan subcutaneously into the plantar portion of the right hind paw of each rat under light anesthesia. Initial foot paw volume is measured immediately following carrageenan challenge using mercury plethysmography (Buxco Electronics).

Edemia is measured five hours after carrageenan. The difference between the five-hour and the initial paw volume is expressed as delta edema. The delta edema for each test group of animals is used to calculate the percent inhibition of edema achieved by the compound at the test dose compared with the vehicle control group. The ID, (the dose at which swelling is inhibited by 40%) is calculated by probit analysis for the dose at which 40 percent inhibition occurs. ,

ap a a -57-

TABLE II

PP

Example NO. arent) aract?) cre(3)

Example NO- — er 64 100 95 31.8 (1) 39.8 (3) 53.7 (10) 58.8 (30) 108 92 86 44.1 (1) 41.1 (3) 32.1 (10) 40.4 (30) 109 95 89 18.5 (1) 23.5 (3) 36.8 (10) 39.4 (30) 110 67 n(4) 32 93 59 re at 16 ML (1) Percent inhibition of cellular 5-lipoxygenase at 16 uM. (2) Percent inhibition of cellular 5-cyclooxygenase at 16 uM. (3) Percent inhibition of carrageenan footpad edema (CFE) test at various doses (mg/kg) of test drug. (4) N inactive at screening concentration.

Accordingly. the present invention also includes a pharmaceutical composition for treating one of the above diseases Or conditions comprising an antidisease Or anticondition effective amount of a compound of the formula I as defined above together with a pharmaceutically acceptable carrier.

The present invention further includes a method for treating one of the above ~amed diseases OT conditions in mammals, including man, suffering therefrom comprising administering to such mammals either orally or parenterally, preferably oral, a corresponding pharmaceutical composition containing a compound of fqrmula I as defined above in appropriate unit dosage form.

For preparing pharmaceutical compositions from the compounds described by th:s invention, inert, 40 pharmaceutically acceptable carriers can be either solid or liquid. Solid form preparations include powders. tablets. dispersible granules, capsules, cachets, and suppositories. \ro ORIGINAL d

(0 x - 5 8 -

A solid carrier can be one or more substances which may also act as diluents, flavoring agents, solubilizers, lubricants, suspending agents, binders or tablet disintegrating agents: it can also be encapsulating material. In powders, the ca.rier is a finely divided active compound. In the tablet the active compound is mixed with carrier having the necessary binding properties in suitable proportions and compacted in the shape and size desired. The powders and tablets preferably contain from 5 or 10 to about 70 percent of the active ingredient. Suitable solid carriers are magnesium carbonate, magnesium stearate, talc, sugar, lactose, pectin, dextrin, starch, gelatin, tragacanth, methylcellulose, sodium carboxymethylcellulose, a low melting wax, cocoa butter, and the like. The term "preparation® is intended to include the formulation of the active compound with encapsulating material as carrier providing a capsule in which the active component (with or without other carriers) is surounded by carrier, which is thus in association with it. Similarly, cachets are included. Tablets, powders, cachets, and capsules can be used as solid dosage forms suitable for oral administration.

For preparing suppositories, a low melting wax such as a mixture of fatty acid glycerides or cocoa putter is first melted, and the active ingredient is dispersed homogeneously therein as by stirring. The molten homogeneous mixture is then poured into convenient sized molds, allowed to cool and thereby to solidify.

Liquid form preparations include solutions, suspensions, and emulsions. As an example may be mentioned water or water-propylene glycol solutions for parenteral injection.

Liquid preparations can also be formulated in solution in aqueous polyethylene glycol solution. Aqueous solutions suitable for oral use can be prepared by dissolving the active component in water and adding suitable colorants, flavors, stabilizing and thickening agents as desired.

Aqueous suspensions suitable for oral use can be made by dispersing the finely divided active component in water with a / —- 5 9 -— viscous material, i.e., natural or synthetic gums, resins, methylcellulose, sodium carboxymethylcellulose, and other well-known suspending agents.

Also included are solid form preparations which are intended to be converted, shortly before use, to liquid form preparations for either oral or parentera'! administration.

Such liquid forms include solutions, suspensions, and emulsions. These particular solid form preparations are most conveniently provided in unit dose form and as such are used to provide a single liquid dosage unit. Alternately, sufficient solid may be provided so that after conversion to liquid form, multiple individual liquid doses may be obtained by measuring predetermined volumes of the liquid form preparation as with a syringe, teaspoon, or other volumetric container. When multiple liquid doses are so prepared, it is preferred to maintain the unused portion of said liquid doses at low temperature (i.e., under refrigeration) in order to retard possible decomposition. The solid form preparations intended to be converted to liquid form may contain, in addition to the active material, flavorants, colorants, stabilizers, buffers, artificial and natural sweeteners, dispersants, thickeners, solubilizing agents, and the like.

The liquid utilized for preparing the liquid form preparation may be water, isotonic water, ethanol, glycerine, propylene glycol, and the like as well as mixtures thereof. Naturally, the liquid utilized will be chosen with regard to the route of administration, for example, liquid preparations containing large amounts of ethanol are not suitable for parenteral use.

Preferably, the pharmaceutical preparation is in unit dosage form. In such form, the preparation is subdivided into unit doses containing appropriate quantities of the active component. The unit dosage form can be a packaged preparation, the package containing discrete quantities of preparation, for example, packeted tablets, capsules, and powders in vials or ampoules. The unit dosage form can also a" —_ 6 0 - be a capsule, cachet, or tablet itself or it can be the appropriate number of any of these in packaged form,

The quantity of active compound in a unit dose of preparation may be varied or adjusted from IL mg to 500 mg preferably to 1 to 50 mg according to the particular pplication and the potency of the active ingredient. The compositions can, if desired, also contain other compatible therapeutic agents.

In therapeutic use as described above, the dosages may be varied depending upon the requirements of the patient, the severity of the condition being treated, and the compound being employed. Determination of the proper dosage for a particular situation is within the skill of the art.

Generally, treatment is initiated with the smaller dosages which are less than the optimum dose of the compound.

Thereafter the dosage is increased by small increments until the optimum effect under the circumstances is reached. For convenience, the total daily dosage may be divided and administered in portions during the day if desired.

In addition to the compounds of formula I, the pharmaceutical compositions can also contain other active ingredients, such as cyclooxygenase inhibitors, nonsteroidal antiinflammatory drugs (NSAIDs), peripheral analgesic agents such as zomepirac, diflunisal, and the like. The weight ratio of the compound of the formula I to the second active ingredient may be varied and will depend upon the effective dose of each ingredient. Generally, an effective dose of each will be used. Thus, for example, when a compound of the formula I is combined with an NSAID, the weight ratio of the compound of the formula I to the NSAID will generally range from about 1000:1 to about 1:1000, preferably about 200:1 to about 1:200. Combinations of a compound of the formula I and other active ingredients will generally also be within the aforementioned range, but in each case, an effective dose of each active ingredient should be used.

—- 8 1 —- combinations of a compound of the formula I and other active ingredients will generally be in the aforementioned ratios.

MSAIDs can be characterized into five groups: (1) the propionic acid derivatives; (2) the acetic acid derivatives; (3) the fenamic acid derivatives: (4) the biphenylcarboxylic acid derivatives: and (5) the oxicams or a pharmaceutically acceptable salt thereof.

The propionic acid derivatives which may be used comprise: ibuprofen, ibuprufen aluminum, indoprofen, ketoprofen, naproxen, benoxaprofen, flurbiprofen, fenoprofen, fenbufen, pirprofen, carprofen, oxaprozin, pranoprofen, miroprofen, tioxaprofen, suprofen, alminoprofen, tiaprofen, fluprofen, and bucloxic acid. structurally related propionic acid derivatives having similar analgesic and antiinflammatory properties are also intended to be included in this group.

Thus, "propionic acid derivatives" as defined herein are nonnarcotic analgesics/nonsteroidal antiinflammatory drugs having a free -CH(CH4)COOH or -CH,CH,COOH group (which optionally can be in the form of a pharmaceutically acceptable salt group, €.9-¢ ~-CH({CH,)COO Na" or -CH,CH,CO0"Na"), typically attached directly or via a carbonyl function to a ring system, preferably to an aromatic ring system.

The acetic acid derivatives which may be used comprise: / indomethacin, which is a preferred NSAID, sulindac, tolmetin, zomepirac, diclofenac, fenclofenac, alclofenac, ibufenac, isoxepac, furofenac, tiopinac, zidometacin, acemetacin, fentiazac, clidanac, oxpinac, and fenclozic acid.

Structurally related acetic acid derivatives having similar analgesic and antiinflammatory properties are also intended to be encompassed by this group.

A or -_ 6 2 -

Thus, "fenamic acid derivatives" as defined herein are nonnarcotic analgesics/nonsteroidal antiinflammatory drugs which contain the basic structure:

CO

COOH which can bear a variety of substituents and in which the free -COOH group can be in the form of a pharmaceutically acceptable salt group, e.g. -COO Na“.

The biphenylcarboxylic acid derivatives which can be used comprise: diflunisal and flufenisal. Structurally related biphenylcarboxylic acid derivatives having similar analgesic and antiinflammatory properties are also intended to be encompassed by this group.

Thus, "piphenylcarboxylic acid derivatives” as defined herein are nonnarcotic analgesics/nonsteroidal antiinflammatory drugs which contain the basic structure: 9 eS COOH which can bear a variety -f substituents and in which the free -COOH group can be 1 -ne form of a pharmaceutically acceptable salt group. e.3., -COO-Na~.

The oxicams which can oe used in the present invention comprise: piroxicam, sudox.cam, isoxicam, and 4-hydroxyl-1,2- penzothiazine 1,1l-dioxide 4-(N-phenyl)-carboxamide. :

Structurally related oxicams having similar analgesic and antiinflammatory properties are also intended to be encompassed by this group.

GINAL 2»

AD OR

(—

ac? -63-

Thus, "oxicams" as defined herein are nonnarcotic analgesica/nonsteroidal antiinflammatory drugs which have the general formui.a:

TH 0 i

YN

No ,S. CHj

Oo 0 wherein R is an aryl or heteroaryl ring system.

The following NSAIDs may also be used: acemetacin, alminoprofen, amfenac sodium, aminoprofen, anitrazafen, antrafenine, auranofin, bendazac lysinate, benzydamine, beprozin, broperamole, bufezolac, carprofen, cinmetacin, ciproquazone, clidanac, cloximate, dazidamine, deboxamet, delmetacin, detomidine, dexindoprofen, diacerein, di-fisalamine, difenpyramide, emorfazone, enfenamic acid, enolicam, epirizole, etersalate, etodolac, etofenamate, fanetizole mesylate, fenclofenac, fenclorac, fendosal, fenflumizole, fentiazac, feprazone, floctafenine, flunixin, / 15 flunoxaprofen, fluproquazone, fopirtoline, fosfosal, furcloprofen, furofenac, glucametacin, guaimesal, ibuproxam, isofezolac, isonixim, isoprofen, isoxepac, isoxicam, lefetamine HCl, leflunomide, lofemizole, lonazolac calcium, lotifazole, loxoprofen, lysin, clonixinate, meclofenamate sodium, meseclazone, microprofen, nabumetone, nictindole, nimesulide, orpanoxin, oxametacin, oxapadol, oxaprozin, perisoxal citrate, pimeprofen, pimetacin, piproxen, pirazolac, pirfenidone, pirprofen, pranoprofen, proglumeracin maleate, proquazone, pyridoxiprofen, sudoxicam, suprofen, talmetacin, talniflumate, tenoxicam, thiazolinobutazone, thielavin B, tiaprofenic acidd, tiaramide HCl, tiflamizole, timegadine, tioxaprofen, tolfenamic acid, tolpadol, tryptamid, ufenamate, and zidometacin.

) 0° i —-64—

Finally, NSAIDs which may also be used include the salicylates, specifically aspirin, and the phenylbutazones, and pharmaceutically acceptable salts thereof.

Pharmaceutically compositions comprising the formula I compounds may also contain as the second active ingredient, antihistaminic agents such as benadryl, dramamine, histadyl, phenergan, and the like. Alternatively, they may include prostaglandin antagonists or thromboxane antagonists such as theose disclosed in U.S. 4,237,160. They may also contain histidine decarboxylase inhibitors such as o-fluoromethylhistidine, described in U.S. 4,325,961. The compounds of formula I may also be advantageously combined with an Hy or Hp-receptor antagonist, such as for instance cimetidine, ranitidine, terfenadine, famotidine, temelastine, acrivastine, loratidine, cetrizine, tazifylline, azelastine, aminothiadiazoles disclosed in EP 81102976.8 and the like compounds, such as those disclosed in U.S. Patent

Nos. 4,283,408; 4,362,736 and 4,394,508. The pharmaceutical compositions may also contain a K*/HYATPase inhibitor such as omeprazole, disclosed in U.S. Patent No. 4,255,431, and the like. Each of the references referred to in this paragraph is hereby incorporated herein by reference. .

Thus, "acetic acid derivatives" as defined herein are nonnarcotic analgesics/nonsteroidal antiinflammatory drugs having a free —CHyCOOH group (which optionally can be in . the form of a pharmaceutically acceptable salt group, e.g.. -CH;CO0 Nat), typically attached directly to a ring system, preferably to an aromatic or heteroaromatic ring system.

Claims (1)

or) -65- 7 09 3 ’ CLAIM 2

1. A pharmaceutical composition comprising a compound of the formula (I) Y, NN : | —L I Y and pharmaceutically acceptable salts thereof; wherein Ar is (i) phenyl; : (ii) phenyl substituted by from one to three lower alkyl, lower alkoxy, hydroxy, halogen, . trifluoromethyl, NOa, mercapto, or lower alkylthio NRioRia1 wherein Rio and Ria are independently hydrogen, or lower alkyl: / (iii) naphthyl; Po (iv) benzofuranyl: (v) benzol[b)-thienyl; (iv) thienyl: (vii) indolyl (viii) furanyl:

i. (ix) pyridyl: Y and Yi: is oxygen or sulfur; X is sulfur, oxygen, NH or NCHa; and X, is NH or NCH»: with the proviso that Ar is not 3,5-di (isopropyl)- 4-hydroxyphenyl and further if X, is NH, X is 5, and Y SE is 8 then Ar is not 3,5-di (t—butyl)-4-hydroxyphenyl and a ) pharmaceutically acceptable carrier additionally comprising an effective amount of a second active ingredient that is a nonsteroidal antiinflammatory drug: a peripheral analgesic agent; a cyclooxygenase inhibitor: a leukotriene antagonist: an antihistaminic agent; a prostaglandin antagonist or a thromboxane antagonist. : WIACZESLAW ANTIN CETENKO ol DAVID THOMAS CONNOR RODERICK JOSEPH SORENSON PAUL CHARLES UNANGST STEPHEN RUSSEL STABLER (Inventors)