PH26189A

PH26189A - Phosphonium salts

Info

Publication number: PH26189A
Application number: PH37229A
Authority: PH
Inventors: Jurgen Cutze; Christo Drandarevski; Guido Albert; Arthur Albert Ramsey
Original assignee: Shell Agrar Gmbh & Co Kg
Priority date: 1987-07-18
Filing date: 1988-07-14
Publication date: 1992-03-18
Also published as: DE3887582T2; DE3887582D1; CN1032794A; EP0300574A2; EP0300574A3; DE3723913A1; PT88005B; CN1020392C; JPH0193596A; EP0300574B1; ATE101157T1; MX172679B; US4933328A; NZ225429A; ZA885127B; PT88005A

Abstract

The invention provides fungicidal compositions containing phosphonium salts of formula:- <CHEM> in which n represents 0, 1, 2 or 3, R represents a halogen atom or an optionally substituted alkyl, haloalkyl, alkoxy or haloalkyl group, R<1>, R<2> and R<3> independently represent an optionally substituted alkyl, cycloalkyl, phenyl or benzyl group and X represents an anion; certain novel phosphonium salts, a process for the preparation of such compounds and a method of combating plant pathogenic fungi using such compositions or compounds.

Description

. Zs PATENTS TEN

D> (UANOLOGY Thay 4 2 (FF am be 27) ns

NX Re 5 a Xs \ econ wi ¥ 5 26189

Co T 915 FF

PHOSPHONIUM SALTS

This invention relates to fungicidal compositions containing phosphonium salts, certain , novel phosphonium salts, a process for the preparation of such compounds and a method of combating plant pathogenic fungi using such compositions or compounds.

Liebigs Ann. Chem., (1981), 623-632 discloses, inter alia, 2-ethoxycarbonylthiazol-4-ylmethyl (tri- phenyl) phosphonium bromide and 2-phenylthiazol-4~ ylmethyl (triphenyl)phosphonium bromide. However, there is no indication in this document that either of these compounds have any fungicidal activity.

It has now been discovered that useful fungicidal activity is present in certain thiazol-4- yl phosphonium salts, some of which are novel.

According to the present invention there is therefore provided a fungicidal composition which comprises at jeast one carrier and, as active ingredient, a compound of the general formula

PsS08011

- 2 = ! ! ® ; / 7 wld el v \ (R),, rR’ in which n represents 0, 1, 2 or 3;

R represents a halogen atom or an optionally substituted alkyl, haloalkyl, alkoxy or haloalkoxy group; rY, Rr? and Rr? independently represent an optionally substituted alkyl, cycloalkyl, phenyl or benzyl group; and

X represents an anion.

When any of the aforementioned compounds contain an alkyl, haloalkyl, alkoxy or haloalkoxy substituent group, this may be linear or branched and may contain up to 12, preferably up to 6, and especially up to 4, carbon atoms.

The terms "halogen" and "halo-" encompass fluorine, chlorine, bromine and iodine, the first three, that is, fluorine, chlorine and bromine, being preferred., - } When any of the foregoing substituents are designated as being optionally substituted, the substituent groups which are optionally present may be any one or more of those customarily employed in the development of pesticidal compounds, and/or the modification of such compounds to influence their structure/activity, persistence, penetration or other property. Specific examples of such substituents include for example halogen atoms, nitro, cyano, hydroxyl, alkyl, haloalkyl, alkoxy, haloalkoxy, pPsS08011 vo _ 3 - amino, alkylamino, dialkylamino, carbonyl, alkoxycarbonyl, carboxyl, alkanoyl, alkylthio, alkylsulphinyl, alkylsulphonyl, carbamoyl, alkylamido, cycloalkyl and phenyl groups. When any of the foregoing substituents represents or contains an alkyl substituent group, this may be linear or branched and may contain up to 12, preferably up to 6, and especially up to 4, carbon atoms.

Preferably, R represents a halogen atom, a C,-6 alkyl or haloalkyl, particularly a Ci-4 alkyl or haloalkyl, group or a Ci-6 alkoxy or haloalkoxy, particularly a Ci-4 alkoxy or haloalkoxy, group. If n represents 2 or 3, the substituents R may be the same or different.

It is preferred that rY, rR? and Rr’ independently represent a C-6 alkyl, particularly a Ci-a alkyl, : group, a Cig cycloalkyl, particularly a Cig cycloalkyl, group or a phenyl group optionally substituted by one or more substituents selected from halogen atoms, Ci-4 alkyl, Ci-4 haloalkyl, Ci-a alkoxy and Ci-4 haloalkoxy groups.

Anion X is preferably derived from a strong organic or inorganic acid, halogen anions such as chlorine and bromine ions being particularly preferred. : A particularly preferred sub-group of compounds of formula I is that in which n is 0, 1 or 2; R represents a chlorine atom or a methyl, trifluoromethyl, methoxy or trifluoromethoxy group; rY, R® and r> independently represent an ethyl, butyl, cyclohexyl, phenyl, fluorophenyl, chlorophenyl, methylphenyl, trifluoromethylphenyl or methoxyphenyl group: and X represents a chlorine anion. [2-(4-chlorophenyl)-thiazol-4-ylmethyl]-

PS08011 vo - 4 - triphenylphosphonium salts, that is, compounds in : which n is 1, R is a chlorine atom substituted at the 4-position and rY, RZ and R3 each represent an unsubstituted phenyl group, are especially preferred.

Many of the compounds of formula I are novel, and the invention therefore also extends to,these novel compounds per se. The novel compounds are the phosphonium salts of formula I in which the : substituents are as defined above, with the proviso that, when re, rR? and R’ each represent an unsubstituted phenyl group and n is 0 then X does not represent a bromine anion. ’

The invention also provides a process for the preparation of compounds of formula I, as defined above, which comprises reacting a compound of formula

Ss (AA -L (II)

N

(R) in which n and R are as defined above and L is a leaving group which may be split off as an anion, with a phosphine of formula 1 ar

P—R (III)

NR3 in which RY, R? and R>? are as defined above/

Preferably, leaving group L is chlorine or bromine, especially chlorine.

The reaction may be conveniently carried out in an inert solvent such as acetonitrile, acetone, toluene, dioxane or tetrahydrofuran at a temperature

PS08011 vo Cs - from 50-150°cC. On cooling, compounds of formula I can normally be separated in crystalline form. compounds of formula II may be prepared as follows using conventional methods:- : 5

H.S Ss

Oya £32 (R) (R) hm n n 2 (IV) rd (Vv) +_C1-CH,COCH, C1 4 rong (R) NH, (VI)

Thiobenzamides of formula V can be prepared from benzonitriles of formula IV by reacting the latter compounds with hydrogen sulphide in an oyganic solvent, such as toluene or pyridine, in the presence of a base, such as triethylamine, ideally at a temperature from 20-50°Cc. The compounds of formula

IV are either known compounds or can be prepared from known compounds by processes analogous to known processes. ) Compounds of formula VI can be prepared by reacting thiobenzamides of formula V with 1,3-di- chloroacetone in an inert solvent such as acetone, dioxane or toluene, preferably at a temperature from 10-50°C. The product can be separated from the reaction medium by suction.

Dehydration of compounds of formula VI using conventional dehydration methods, such as, treatment with concentrated sulphuric acid or polyphosphoric

PS08011

~~ 26189 to -6 ~- acid, optionally with heating, produces compounds of formula II (cf. A. Silberg et al., Ber. 94, 2887-94 (1961)).

Phosphines of formula III are known compounds or can be prepared according to Houben-Weyl, Vol. 12/1, pages 17-66, published by Georg Thieme Verlag,

Stuttgart 1963.

The invention also includes a method for making a fungicidal composition as defined above which comprises bringing a compound of formula I into ra association with at least one carrier. /

A composition according to the invention preferably contains from 0.5 to 95% by weight of active ingredient. — oo

A carrier in a composition according to the invention is any material with which the active ingredient is formulated to facilitate application to the locus to be treated, which may for example be a plant, seed or soil, or to facilitate storage, transport or handling. A carrier may be a solid or a liquid, including a material which is normally gaseous but which has been compressed to form a liquid, and any of the carriers normally used in formulating fungicidal compositions may be used.

Suitable solid carriers include natural and } synthetic clays and silicates, for example natural silicas such as diatomaceous earths; magnesium silicates, for example talcs; magnesium aluminium silicates, for example attapulgites and vermiculites; 300 aluminium silicates, for example kaolinites, montmorillonites and micas; calcium carbonate: calcium sulphate; ammonium sulphate; synthetic hydrated silicon oxides and synthetic calcium or aluminium silicates: elements, for example carbon/ and sulphur; natural and synthetic resins, for example

PS08011

' _ coumarone resins, polyvinyl chloride, and styrene polymers and copolymers; solid polychlorophenols; bitumen; waxes, for example beeswax, paraffin wax, and chlorinated mineral waxes; and solid fertilisers, for example superphosphates.

Suitable liquid carriers include water; alcohols, for example isopropanol and glycols: ketones, for example acetone, methyl ethyl ketone, methyl isobutyl ketone and cyclohexanone; ethers; aromatic or araliphatic hydrocarbons, for example benzene, toluene and xylene; petroleum fractions, for example kerosine and light mineral oils; chlorinated hydrocarbons, for example carbon tetrachloride, perchloroethylene and trichloroethane. Mixtures of different liquids are often suitable.

Fungicidal compositions are often formulated and transported in a concentrated form which is subsequently diluted by the user before application.

The presence of small amounts of a carrier which is a surface-active agent facilitates this process of dilution. Thus preferably at least one carrier in a composition according to the invention is a surface-active agent. For example the composition _- may contain at least two carriers, at [least one of “25 which is a surface-active agent.

A surface-active agent may be an emulsifying agent, a dispersing agent or a wetting agent; it may be nonionic or ionic. Examples of suitable surface- active agents include the sodium or calcium salts of polyacrylic acids and lignin sulphonic acids; the condensation products of fatty acids or aliphatic amines or amides containing at least 12 carbon atoms in the molecule with ethylene oxide and/or propylene oxide; fatty acid esters of glycerol, sorbitol, gucrose or pentaerythritol; condensates of these with

PS08011

Co 26189 vo _ 8 - Co ethylene oxide and/or propylene oxide; condensation products of fatty alcohols or alkyl phenols, for example p-octylphenol or p-octylcresol, with ethylene oxide and/or propylene oxide: sulphates or sulphonates of these condensation products; alkali or alkaline earth metal salts, preferably sodium salts, of sulphuric or sulphonic acid esters containing at jeast 10 carbon atoms in the molecule, for example sodium lauryl sulphate, sodium secondary alkyl gulphates, sodium salts of sulphonated castor oil, and sodium alkylaryl sulphonates such as dodecyl- : benzene sulphonate; and polymers of ethylene oxide and copolymers of ethylene oxide and propylene oxide.”

The compositions of the invention may for example be formulated as wettable powders, dusts, pastes, granules, soluble powders, solutions, emulsifiable concentrates, emulsions, suspension concentrates, suspensions, and aerosols. Wettable powders usually contain 25, 50 or 75% w of active ingredient and usually contain in addition to solid inert carrier, 3-10% w of a dispersing agent and, where necessary, 0-10% w of stabiliser (s) and/or other additives such as penetrants or stickers.

Dusts are usually formulated as a dust concentrate having a similar composition to that of a wettable powder but without a dispersant, and may be diluted ’ in the field with further solid carrier to give a composition usually containing 1-10% w of active ingredient. Granules are usually prepared to have a size between 10 and 100 BS mesh (1.676 - 0.152 mm) , and may be manufactured by agglomeration or impregnation techniques. Generally, granules will contain 1-75% w active ingredient and 0-10% w of additives such as stabilisers, surfactants, slow release modifiers and binding agents. The so-called

PS08011

’ . Co "dry flowable powders" consist of relatively small granules having a relatively high concentration of active ingredient. Emulsifiable concentrates fusually contain, in addition to a solvent and, when necessary, co-solvent, 1-50% w/v active ingredient, 2-20% w/v emulsifiers and 0-20% w/v of other additives such as stabilisers, penetrants and corrosion inhibitors. Suspension concentrates are usually compounded so as to obtain a stable, non-sedimenting flowable product and usually contain 10-75% w active ingredient, 0.5-15% w of dispersing agents, 0.1-10% w of suspending agents such as protective colloids and thixotropic agents, 0-10% w of other additives such as defoamers, corrosion inhibitors, stabilisers, penetrants and stickers, and water or an organic liquid in which the active ingredient is substantially insoluble; certain organic solids or inorganic salts may be present dissolved in the formulation to assist in preventing gedimentation or as anti-freeze agents for water.

Aqueous dispersions and emulsions, for example compositions obtained by diluting a wettable powder or a concentrate according to the invention with water, also lie within the scope of the invention.

The said emulsions may be of the water-in-oil or of the oil-in-water type, and may, have a thick ’ 'mayonnaise'-like consistency

Other suitable formulations include aqueous or organic solutions encapsulated in polymers, encapsulated powders and natural and synthetic materials or carriers impregnated with the active ingredient. on account of the ionic structure of the active ingredients, particular care must be taken that the other ingredients in the formulation are compatible

PS08011 with the active ingredients, especially if they also have an ionic structure (e.g. dispersants such as substituted naphthalene sulphonic acid-formaldehyde condensates, lignin sulphonates, polyacrylates, phosphates and sulphates of ethoxylated fatty acids and phenols, or wetting agents such as sodium dioctyl sulphosuccinate, alkylnaphthalene sulphonates, substituted and unsubstituted fatty acid taurides and quaternary ammonium compounds). Non-ionic auxiliary substances are to be preferred.

Formulation Example

Emulsion concentrate

Composition:-

Active ingredient according to the invention 200 g/1

Ethoxylated castor oil 100 g/1

Tetrahydrofurfuryl alcohol 793 g/1

Density 1.093 g/ml

The composition of the invention may also contain other ingredients, for example other compounds possessing herbicidal, insecticidal or fungicidal properties.

Of particular interest in enhancing the duration of the protective activity of the compounds of this invention is the use of a carrier which will provide a glow release of the fungicidal compounds into the environment of the plant which is to be protected.

Such slow-release formulations could, for example, be inserted in the soil adjacent to the roots of a vine plant, or could include an adhesive component

P5S08011 to -11 - enabling them to be applied directly to the stem of a vine plant.

The invention still further provides the use as a fungicide of a compound of the general formula I as defined above or an acid-addition salt or metal salt complex thereof, and a method for combating fungus at a locus, which comprises treating the locus, which : may be for example plants subject to or subjected to fungal attack, seeds of such plants or the medium in which such plants are growing or are to be grown, with such a compound.

The present invention is of wide applicability in the protection of crop plants against fungal attack. The compounds and compositions of the invention are particularly active against botrytis, especially botrytis cinerea, and may be used in all crops where botrytis attack is undesirable. Typical : crops which may be protected include vines, tomatoes, strawberries, beans and ornamentals. The duration of protection is normally dependent on the individual compound selected, and also a variety of external factors, such as climate, whose impact is normally mitigated by the use of a suitable formulation.

The invention is further illustrated by the following Examples.

Example 1

Preparation of [2-(4-chlorophenyl)-thiazol-4-yl=- methyl ]-triphenylphosphonium chloride (a) 4-chlorothiobenzamide 68.8 g (0.5 mol) of 4-chlorobenzonitrile were dissolved in a mixture of 100 ml pyridine and 50 ml triethylamine. Whilst stirring, an even flow of 15 1 (0.67 mol/hour) of hydrogen sulphide was introduced at room temperature, then 11 1 (0.5 mol/hour) for 30

PS08011 bo -12 - , min at 50°¢, i.e. a total of 1.25 mol. This/was then stirred for a further 30 min at 50°C and then mixed into 1.5 litres of water. The crystallate was drawn off, washed with plenty of water and dried.

Yield: 81g (94% of theory); m.pt. 129°C. (b) 4-chlorothiobenzimidic acid-3-chloroacetonyl ester hydrochloride 8.58 g (50 mmol) of the 4-chlorothiobenzamide obtained in (a) were dissolved in 35 ml acetone, 6.35 g (50 mmol) of 1,3 dichloroacetone were added and left to stand for 1 day at room temperature. The crystallate was drawn off, washed with acetone and dried. yield: 12.2g (82% of theory); m.pt. 149°C (decomposition). (c) 4-chloromethyl-2-(4-chlorophenyl)-thiazole 11.95 g (40 mmol) of the 4-chlorothiobenzimidic acid-3-chloroacetonyl ester hydrochloride obtained in (b) were introduced into 40 ml concentrated sulphuric acid at room temperature, with stirring, left to stand at room temperature for 30 minutes and then poured onto ice. The crystallate was drawn off, washed free of acid with water, and dried.

Yield: 9.6g (98% of theory): m.pt. 81°¢c. (4d) [2-(4-chlorophenyl)-thiazol-4~-ylmethyl]-tri- ) phenyl-phosphonium chloride 2,44g (10 mmol) of the 4-chloromethyl-2-(4-chloro- phenyl) -thiazole obtained in (c), and 2.62g (10 mmol) of triphenyl phosphine were refluxed with 20 ml of acetonitrile for 3 hours, whereupon the substance precipitated out in crystalline form. After cooling,

PS08011 tf 26189 ' - 13 - jt was drawn off and washed with acetone and then dried. yield: 3.65g (72% of theory), m.pt. 308-310°C.

Examples 2 to 34

Following procedures similar to those described in Example 1 above, further compounds according to the invention were prepared as detailed in Table I below. In this table, the compounds are identified by reference to formula I.

PS08011

' . } Q Q OU 0 Q Q <r ~ ~ ~ a 1 [oN © © 0 « un ~ ™ © . o [=] oN NH © 0 <3 © = oN oN oN oN oN o~N — ™ >< ~ — ~ — — — ~ — 0 oO QO 0 O U 0 Q ~ ~— co > o a 4 — — —4 — —~ on Q [aH > > > > > fe of £ ™ © = = 5 o | DM ~ Oo Q Q aU a Q Q © | L £ L Ko 1 © wn a Pe a Ae [a7 a foo]

OQ 0 1 1 << « —

Sy — c >

Q o o —~ — — — — on 0 } Ay > > > > > jo) ££ oN o o = c a + ~ Oo Q Q Q a Q Q mm | Oo Ll L L 1 © [. rd Ay Pa ny Pe [a1 = on 0 0 o I ! rH « < 0 0 } EH ~~

Sy — c >

I) = £8 — — ~~ — — on Q

Ay > > > > o> mn ££ —~ a =o = a oc < | fA ~ Oo a Qo Q Qo Q oO a | L £8 L 1 ™m on Ma ny ny on «1 a wm oO 0 i 1 <$ ho oN oO . ™ © © joo] xy wm ~ ~ — « 0 EET IRE |B 1 < < oN A) << <¥ ™ — = oo o oN ~{ — — — — 3 © oO 0 wn fr al jo] g oN © « Ts) © t~ © on

QS

» O [23 JA

, . 0 0 0 0 Q 9

Is 0, © Nn oN a ~ 0 © © . © 0 ~~ o ~N 0 ~ — = ~ oN ~N a o~ oN oN o~ — —~ — ~ — — ~ ~ 0 Q O © Q oO Q 0 — ~~

Sy — — ~ > ~~ ~ o > > > co > » —

Q 0 co o Q c o > 0 Q Q Q Ke] ao Q c ny =] L L (a¥) £8 Ko] Q ™ Ay Ay Py Ay A 0 ox oO Oo on . © 4 ~~ ™ © ™ ™ on O © wo od om on fre oO | | Q QO 0 0 I [sa] <3 I | 1 1 «

Cop < A © ™ 4 — oa —t — — Dy — — o > > > a > > ~~ — 0 =! £ co 0 g o > o L [1 a Q Ll Q a =

Q [sh Ke] ef ns Li £ o

J ™ [a¥ [oH om ny 0 Ko] g 7 o o Au -— © 4 ~~ © © ™ © + on 0 0 wn on 0 on Fea = Q 1 I 0 Q 0 Q 0 I © < | 1 1 ' << ! oO < A) Sa © ©

H

0 ~ ~ — Sy — ~ — > ~~ —

Q = > > > a > > ~~ © Q o a oc o a o >

BE L a [] Q fo] Q Q o ny io] L Li an L Q — oY iY A [=H ~ £ x o o A ™m ~ — ™ ™ ™ © ow 0 Q wo jest wn mw fr oO 1 i 0 oO 8) oO ! 1 ul < 1 | | t Sy << Ay < © ™ . ™ ™ ™ © — — —~ Jos ae] — 5 fe ol ¢ 19 |? |F |Y | 7 << + <¥ Ld << + A < — c — — — ~ —~ — — — J © o 0 9° [a¥ jo 8 Oo ~~ oN ™ <P un 0 ~~ © —t — i —~ —~ — — — © 0

KO

» vu, 1

QO

0 i) 0, ~~ [e] © ~ oN . 0 ™m < 0 ~ = oN oN oN 1 ~ — —~ — ~ — ~ ~~ —~

QO Q Q 0 0 OQ oO QO —~ — > —~ oN om 2}

S118 |2 (2 |= © Ll io] L o a Ad A) Sy mM oO 0 a Q Q oO Q OQ ~— — L io] 1 | 1 0 0 fr Ay [a¥ = cs = > > 1

QO Q <r — — — od 2 o or & oc ot ~~ ~~ ~~ Q h) i} ] Q > > Lo > £ 9 a No L Ke oS o a = 0 = ~ Oo 0 Py Q a Q Q 4 — — L Li L Ll — + 0 0 fy [s) [e¥ [s1 Mn Q o > > I 1 0 Q QO t L 0

HH a — —

Q —t —~ — >

Io Be er > & = a ~~ — ~~ ~ Q i) o 0 > > > > £ ~ Kea] Ke Ko] o o a c ~ ~ 0 Oo A Q Q Q Q — — of Li Li Ke] — 0 0 x [sN) [o mn (a1) 0

Dy > 1 ! oO QL <r hd : © on | O ™ — m — +9 ry | oi ~~ (84 QO O oO Q By O Q 0 i ! © | 1 1 <t < Loy <r | << < <r < —~ a — — — ~ ~ ~ — —~ 0 a a — Pa jo} g w oN oO — oN © 4 wn re — ~ oN oN oN oN oN oN

XX 0 mx = r oO 0 is} jo] © a — . o ~ o = ~ — ~ 4 ~~ — ~~ ~~ — —t ~~ —

Q O 0 OL oO Q oO oO on on oO oO on — be of on o 21 wl mn in Sy ™ <r <r Nd < «t mo. lm c ~ O O 0 O 0 ND ~ Q 1 I 1 0 O Kel = = = = = aN — & a a — — — ~ — — — a O > > > > > > > 3 o~ Ay a © o of o o a a [34 Q Q a Q Q [4] Q -— © Ke L £ ££ L L £ » fry A Mn Ay Ry Mu Pe 2 = 0

Q ! r Q <* a . Q —~ —

Q > « a = 0 —~ —t — ~- — —t —t £ ™ > > > > > > — A a a [=] G5 o 5 = 0" o a Q a a Q a “ £ LS (| & Li Lo. No} £0 [9 on o [oY [oh ny on Py oO 1 i + . © oO Oo © © [<n © ™ ~~ oi xo ~ ' | Q x4 oa 0 QL OQ 1 oO QO ) <r ' | << << < <« < . ~ o o o ~ — ~ —~ ~ ~ 3 © @

JS a 0, g wo ™~ «© oO o ~ ~ © lu] * oN oN oN oN © ™ © © x O mn =

. oo 0 0

FS) [oH (o} . ~~ = o~ » — oO — > ] = ~ Q 0 ny a —

Q > 3 |x a = 0 Q -~ L 3 a = 0

QO

CH

. Q —t

Q

[1s]

EH — > ~ o ~ Q

Fo]

Ay — “= |% <r - o — . ° . 0 o 3 a 2

E A 4 ag ™ x O

Ku = v 26189 bo - 19 -

Example 35

The fungicidal activity of representative compounds of the invention was investigated by means of the following test. protectant activity against vine grey mould (Botrytis cinerea)

The test is a direct protectant one on the grape itself. As Botrytis cinerea normally infects grapes which have a certain sugar content, grapes are selected which have between 60 and 90 Ochsle degrees. (An Ochsle degree is a measure of the specific density of the grape juice and the sugar concentration can be calculated from this). It is . also important to ensure that neither the grapes nor the stems are damaged or show any signs of necrosis.

Selected grapes are cut off about 5mm above the start of the stem and then dipped in a solution comprising 500ppm of the test compound in water, acetone, methanol or a mixture thereof, the solvent or solvent mixture also containing 500ppm Triton

X-100. The cut is then sealed with paraffin wax.

Four controls are also set up in which grapes are treated with the following substances:- (1) H,0 = distilled water (2) LA 0.5 = 10% acetone solution containing 500 ppm

Triton X-100 (3) LM 0.5 = 10% methanol solution containing 500 ppm Triton X-100 (4) LW 0.5 = distilled water containing 500 ppm

Triton X-100

The grapes are divided up according to treatment and cet out in blocks on a stainless steel grid at room temperature. 30-50 grapes are used per treatment according to the homogeneity of the grape material.

X

PS08011

After 2 days, the treated grapes are sprayed with an aqueous spore suspension of a field isolate of

Botrytis cinerea containing 100 000 spores/ml using a spray gun. The grapes are then incubated in a humid room (100% relative humidity) at 20-22°c. The infected grapes are counted at various successive times according to the development of the infection and the Abbott efficiency is then calculated as follows: ~-

AW

Efficiency E (%) = 100 ~- > BB, x 100 i=aW, Ky th where AW =n evaluation

AW, = 15t evaluation

BB; = no of infected grapes in ith evaluation

K; = mean value of no. of infected grapes ins all (i.e. H,0, IA, LW and LM) controls in ith evaluation

The results of tests carried out according to the above method are set out in the following tables.

In these tables, the additional abbreviations BBx and

Ex are used where x is the time in days between infection and evaluation.

PS08011

™ € ¢ — oo Oo oO Oo oO © Oo oO - = a N —~ oO OO O Oo Oo © oo x — ~~ co oo oo oO o oo ml t~ oOo oo o © oO m Oo © = — 0 oo o o o -H <4 0 A m ~ <1 oo 1n © © oo oO Vw nM [oa] "Qo oOo oo o © © OV TT © fi] ~ 0 O OO

I —

IU DE

N oO —~ m oo © o © Oo 0 Oo oO m oN —~ m oo o o oO oO oo o oo m — : —~ m oO © OO © oO ~ O © m ~~ m nN Oo HO + mm OO m \ 0 m in << << 0 + MO 0 m — — m + + O « n - © © m ~~ A 4 <t m oO in Nn oO ~~ nN O A m pu — m : o 0 ~ - 0 © oO 0 oO

Be 0 — ~ o ao Hh 0 0 O 3 > ae 2 A HH a £ cP PL g — 0

Bz = 0 S558 og km [ml

0 : oO OO Oo oOo 9 << Mm ~ O ~~ 0 26189 3] I 1 A oN nN oO ' nn © Oo Oo © OM NN 0 NN © MO > x] mE NT Nn ©o©0 ’ < © OO 0 © MF NT NNO mw Mm MOF OF 00 O ’ —~ © 0 Oo Oo oO nN 4 OO Om OO © 3] 4 O00 O OO OO Ov © O [= BT ~ A 0 © tn Nn Mm OO VU ¥ O VW mm O m — — —~ m nn

A ~~ mn N O VU ID VW +H OO © m ~ m 4 nn << OO OV OO Mm © Mm Mm +H O m — ~ - m © n Oo nn © oC OO OO 0 OO A O © m ~N NH © m a

Oo -~

Oo 0 Oo OO 0 0 Oo ~ BE oo Oo OO ie Qu UU VU oO + 0 8 BE BE BE BE BEB 12] I 8 oO ££ od od od © © ol O O 0 NE ogo O 0 X¥ MX HX XX xX x

Hl =z = O mo MoM a RR

9 Oo OO oO oO IAL vo oo OO OO oO << oO

Tr 1 oO OO Oo oO Ov oN 0 vv Mm Ww \0 << OW In Mm ON ON m m wn ~~ 0 ~~ -I WO « m — ~~ NH m ha nn OV << << n oO In 0 —_H HoH — m { c

Oo 4

ES] Oo oO ©O

Bloz = 0 S558 fm fd

<r ~~ oO Oo © © A In ~4 nn 0 Wn “ 9 mn 1 1 mn — oO oOo oOo © ~ 10 ~ ID oN In vo i ' i « ~~ — 0 0 Oo oO - Ov 4 10 WV Wn =? 1 1 < A o — oO 0 Oo o ~ OH OV NO f=] I I ~ ID oN ~ oo Oo Oo o ™m oo mom oo ~

Kd 1 aN 1 nw wv © Oo Oo © <¢ MN O «1 In m ~ ® a 0 av n OO OO 0 © OO OO OO OO 0 © fxd oO On O OO Oo ~ ~~ I +A ~ A — a oO 0 oo oO oO oo oo HA Oo oN — 0 oO oo o o oO +4 OO OO +H O — . — 0 © oo Oo oO OO OO O « oN OM ; oO ~~ m oc oo +A ~ oO Vv O NN WV In m — t~

Mm oO OW WV oO ~~ NN OO m ~ ~A — 0 mM + 0 VW © Oo < NN NH © m NH HN ~ ~N 0 : o Ow MNT ON © 0 VW Oo Oo © . o 0 ~ -~ is} OO OO OO OO i « nn wn «+N — ~~ — 21 | JRO © § | E 53 nn -A 0 + = n 0 8 ~ 0 — — pa © © 0 ~ —~ o

Hl == oO m3 CT

« ~ oOo oo o o aD Mm oO O 11 1 0n eo [2a] yo, ] oN o~ oO © o © Nn ~H ID O OV O 1 11 Vv On oN a © — oO o o oO AH NY MY 1 1 OV In mn

I~ ~~ m — ~ oo oo o © Nn oOo HBO f —~ © I~ OV m 1 0 oo oo o © ~~ Hn i! © A OO © Oo 0 <r oo o oOo Oo mM oOo nN ©O © © ~ ® 8 ©O O O mM | tf ~~ a 0 - 2 oO Oo oo o oO « tn 0 ne ee

N ~~ ~ |Z £ EE

B 58 5 ou Fx mn a 0 —,

A

0

Q 4 a

Qu — 5 rr 0 4 ~ in OW oO °

VR 4 0 0 O ~~ © on 0 o DP Hoan a = cp PP a ~~ 0 0 0 @n 0] Wow 3 oO £ E & © 8 2d dD ou qu 5 O 0 0 O ~- 0 BE Bf 2 { E oO £2 ~ ££ © © ol o © 0 oz og om | 908 RES ae 2 o ls le mom [a WE

Claims

1. A compound of ths goneral formula @ rt N -- cl, =—= P—R © (1) N \ 3 ; (R) R in which n represents 0, 1 OF 23 R represents a halogen atom or a Cc, _g2lkyl, c,_ghaloalkyl, Cy Lgalloxy 0 :,_ haloalkoxy group; ’ rR", rR’ ana 1 JuQependently represent = hanryl, C,_galkyl or C,_geycloalkyl group or a phenyl group optionally substituted by one or more gupetituenta selected from halogen atons, Cc, _,2lkyl, c,_ haloalkyl and C)_42lkoxy groups? and X represents an anion; with the proviso that when rt, Rr? and R° each represent an unsubstituted plitnyl yLroup and ny de 0 than X does not represent a bromine anion. 1v07077 BAD ORIGINAL

, BE

2. A compound according to claim 1 in which X : represents a halogen anion.

3. A compound according to ¢laim 1 in which R represents a chlorine atom ar a methyl, trifluoromethyl, methoxy Of tel tuoromethoXy ‘group rt, Rr? and rR’ independently represent an ethyl, butyl, cyclohexyl, phenyl, benzyl, fluorophenyl, chloxrophenyl, methylphenyl, trifluoromethylphenyl or methoxyphenyl groupi and X represents a chlorine anion.

4. A method of combating fungus at a locus which comprises treating the Jocus with a Fungicidally effective amount of a compound according to claim 1. Oa A mathed agepxding ko intm 4, wherein the lc 0 gonprises plants subject to or gubjected to fungal attack, seeds of such plants, Or the medium in which the plants are growing or are to be grown. BAD ORIGINAL 07077 nr fe bal pre VYAHES coe] 68. NL FL