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Application filed by Illumina IncfiledCriticalIllumina Inc
Publication of PH12020552299A1publicationCriticalpatent/PH12020552299A1/en
Measuring Or Testing Involving Enzymes Or Micro-Organisms
(AREA)
Abstract
Provided is a device, including a conductive channel and a number of polymerase molecules bound thereto, wherein the number is between one and five and the conductive channel is to detect incorporation of a nucleotide comprising a charge tag into a nascent polynucleotide by the polymerase, and each of the one or more polymerase molecules includes a hexahistidine tag, the conductive channel includes a multivalent nickel-nitrolotriacetic acid complex, and the hexahistidine tag is bound to the multivalent nickel-nitrolotriacetic acid complex. Also provided are a method of attaching a polymerase to a conductive channel of a device and a method of using the device.
PH12020552299A2019-06-182020-12-23Polymerase attachment to a conductive channel
PH12020552299A1
(en)
Use of micro-rnas or genes as markers for the identification, diagnosis and therapy of individual forms of non-ischemic cardiomyopathies or storage diseases of the heart