PH12020552299A1 - Polymerase attachment to a conductive channel - Google Patents

Polymerase attachment to a conductive channel

Info

Publication number
PH12020552299A1
PH12020552299A1 PH12020552299A PH12020552299A PH12020552299A1 PH 12020552299 A1 PH12020552299 A1 PH 12020552299A1 PH 12020552299 A PH12020552299 A PH 12020552299A PH 12020552299 A PH12020552299 A PH 12020552299A PH 12020552299 A1 PH12020552299 A1 PH 12020552299A1
Authority
PH
Philippines
Prior art keywords
conductive channel
polymerase
multivalent
nickel
polymerase attachment
Prior art date
Application number
PH12020552299A
Inventor
Yannan Zhao
Emily Welch
Original Assignee
Illumina Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Illumina Inc filed Critical Illumina Inc
Publication of PH12020552299A1 publication Critical patent/PH12020552299A1/en

Links

Landscapes

  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Provided is a device, including a conductive channel and a number of polymerase molecules bound thereto, wherein the number is between one and five and the conductive channel is to detect incorporation of a nucleotide comprising a charge tag into a nascent polynucleotide by the polymerase, and each of the one or more polymerase molecules includes a hexahistidine tag, the conductive channel includes a multivalent nickel-nitrolotriacetic acid complex, and the hexahistidine tag is bound to the multivalent nickel-nitrolotriacetic acid complex. Also provided are a method of attaching a polymerase to a conductive channel of a device and a method of using the device.
PH12020552299A 2019-06-18 2020-12-23 Polymerase attachment to a conductive channel PH12020552299A1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201962862767P 2019-06-18 2019-06-18
US2020000373 2020-06-12

Publications (1)

Publication Number Publication Date
PH12020552299A1 true PH12020552299A1 (en) 2021-09-27

Family

ID=77989530

Family Applications (1)

Application Number Title Priority Date Filing Date
PH12020552299A PH12020552299A1 (en) 2019-06-18 2020-12-23 Polymerase attachment to a conductive channel

Country Status (1)

Country Link
PH (1) PH12020552299A1 (en)

Similar Documents

Publication Publication Date Title
EP4303314A3 (en) Polypeptide tagged nucleotides and use thereof in nucleic acid sequencing by nanopore detection
PH12016502542A1 (en) Allele-specific amplification using a composition of overlapping non-allele-specific primer and allele-specific blocker oligonucleotides
MX2016007541A (en) Modified nucleic acid molecules and uses thereof.
MX2018010633A (en) Closed-ended linear duplex dna for non-viral gene transfer.
MX2018000729A (en) Methods of amplifying nucleic acid sequences.
PH12016500660A1 (en) Substituted nucleosides, nucleotides and analogs thereof
WO2017156015A3 (en) Micrornas and methods of their use
WO2016028843A3 (en) Rna-guided systems for probing and mapping of nucleic acids
PH12015501423A1 (en) Substituted nucleosides, nucleotides and analogs thereof
WO2015170323A3 (en) Compositions and methods of using same for reducing resistance to mosquito larvicides
WO2014138119A3 (en) Method and kit for characterizing microorganisms
WO2013056241A3 (en) Real-time redox sequencing
NZ590750A (en) Methods and compositions for improving the production of products in microorganisms, especially clostridium phytofermentans
NZ740630A (en) Polymethine compounds and their use as fluorescent labels
GB2529089A (en) Learning health systems and methods
WO2013127782A3 (en) Use of micro-rnas or genes as markers for the identification, diagnosis and therapy of individual forms of non-ischemic cardiomyopathies or storage diseases of the heart
WO2015114469A3 (en) Covered sequence conversion dna and detection methods
EP3942066A4 (en) Nucleic acid hybridization methods
WO2014005125A3 (en) Fluorescent compounds and uses thereof
PH12021551087A1 (en) A peripheral blood mirna marker for diagnosis of non-small cell lung cancer
MX354465B (en) Detection of nucleotide variation on target nucleic acid sequence by pto cleavage and extension assay.
EP3760713A3 (en) Lipase variants and polynucleotides encoding same
MX2017009542A (en) Branched and linear chimeric compounds, polynucleotides, uses and methods for preparation thereof.
WO2010109463A3 (en) Methods of predicting pairability and secondary structures of rna molecules
WO2016154040A3 (en) Biosynthetic labeling and separation of rna