PE20210551A1 - Metodos para prevenir el enlace disulfuro en la cosecha del cultivo celular con selenito - Google Patents
Metodos para prevenir el enlace disulfuro en la cosecha del cultivo celular con selenitoInfo
- Publication number
- PE20210551A1 PE20210551A1 PE2020000384A PE2020000384A PE20210551A1 PE 20210551 A1 PE20210551 A1 PE 20210551A1 PE 2020000384 A PE2020000384 A PE 2020000384A PE 2020000384 A PE2020000384 A PE 2020000384A PE 20210551 A1 PE20210551 A1 PE 20210551A1
- Authority
- PE
- Peru
- Prior art keywords
- selenite
- harvest
- methods
- cell culture
- cell
- Prior art date
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
- C07K1/113—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure
- C07K1/1133—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure by redox-reactions involving cystein/cystin side chains
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K39/395—Antibodies; Immunoglobulins; Immune serum, e.g. antilymphocytic serum
- A61K39/39591—Stabilisation, fragmentation
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/145—Extraction; Separation; Purification by extraction or solubilisation
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/06—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies from serum
- C07K16/065—Purification, fragmentation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/999—Small molecules not provided for elsewhere
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- General Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Pharmacology & Pharmacy (AREA)
- Plant Pathology (AREA)
- Mycology (AREA)
- Physics & Mathematics (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Referido a un metodo in vitro para la prevencion de la reduccion de un enlace disulfuro en un polipeptido expresado en una celula huesped recombinante, que comprende, despues de la fermentacion, agregar selenito, sus sales o derivados, a una solucion de recoleccion de la celula huesped recombinante, en donde el enlace disulfuro en el polipeptido permanece no reducido. En donde el polipeptido comprende un anticuerpo, y en donde la celula huesped comprende una celula eucariota, mas especificamente una celula de mamifero.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201762566817P | 2017-10-02 | 2017-10-02 | |
| PCT/US2018/052798 WO2019070468A1 (en) | 2017-10-02 | 2018-09-26 | METHODS FOR PREVENTING THE REDUCTION OF DISULFIDE BINDINGS IN COLLECTING CELL CULTURE WITH SELENITE |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| PE20210551A1 true PE20210551A1 (es) | 2021-03-17 |
Family
ID=64049678
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PE2020000384A PE20210551A1 (es) | 2017-10-02 | 2018-09-26 | Metodos para prevenir el enlace disulfuro en la cosecha del cultivo celular con selenito |
Country Status (15)
| Country | Link |
|---|---|
| US (1) | US11555052B2 (es) |
| EP (2) | EP3692062B1 (es) |
| JP (1) | JP2020536083A (es) |
| KR (1) | KR20200058426A (es) |
| CN (1) | CN111164102A (es) |
| AU (1) | AU2018345510A1 (es) |
| BR (1) | BR112020006476A2 (es) |
| CA (1) | CA3077497A1 (es) |
| IL (1) | IL273302A (es) |
| MX (1) | MX2020003013A (es) |
| PE (1) | PE20210551A1 (es) |
| RU (1) | RU2020115258A (es) |
| SG (1) | SG11202002121TA (es) |
| TW (1) | TW201923069A (es) |
| WO (1) | WO2019070468A1 (es) |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20020032315A1 (en) | 1997-08-06 | 2002-03-14 | Manuel Baca | Anti-vegf antibodies |
| US20040029229A1 (en) | 2002-05-20 | 2004-02-12 | Reeves Philip J. | High level protein expression system |
| SG156672A1 (en) | 2004-10-22 | 2009-11-26 | Amgen Inc | Methods for refolding of recombinant antibodies |
| KR101643514B1 (ko) | 2007-07-09 | 2016-07-27 | 제넨테크, 인크. | 폴리펩티드의 재조합 생산 동안의 디술피드 결합 환원의 방지 |
| AU2009327411A1 (en) * | 2008-12-19 | 2010-06-24 | Merck Sharp & Dohme Corp. | Feed supplement for mammalian cell culture and methods of use |
| US9976163B2 (en) | 2012-10-03 | 2018-05-22 | Kyowa Hakko Kirin Co., Ltd | Method for preventing reduction of polypeptide by adding amino acid to culture solution |
| JP2016527911A (ja) * | 2013-08-20 | 2016-09-15 | レック・ファーマシューティカルズ・ディー・ディーＬｅｋ Ｐｈａｒｍａｃｅｕｔｉｃａｌｓ Ｄ．Ｄ． | ポリペプチドのα−アミド化および／またはＣ末端アミノ酸開裂を制御するための細胞培養用培地およびプロセス |
-
2018
- 2018-09-13 TW TW107132186A patent/TW201923069A/zh unknown
- 2018-09-26 PE PE2020000384A patent/PE20210551A1/es unknown
- 2018-09-26 US US16/648,014 patent/US11555052B2/en active Active
- 2018-09-26 EP EP18795833.5A patent/EP3692062B1/en active Active
- 2018-09-26 WO PCT/US2018/052798 patent/WO2019070468A1/en unknown
- 2018-09-26 KR KR1020207009069A patent/KR20200058426A/ko not_active Application Discontinuation
- 2018-09-26 MX MX2020003013A patent/MX2020003013A/es unknown
- 2018-09-26 SG SG11202002121TA patent/SG11202002121TA/en unknown
- 2018-09-26 CN CN201880064558.2A patent/CN111164102A/zh active Pending
- 2018-09-26 CA CA3077497A patent/CA3077497A1/en active Pending
- 2018-09-26 AU AU2018345510A patent/AU2018345510A1/en not_active Abandoned
- 2018-09-26 RU RU2020115258A patent/RU2020115258A/ru unknown
- 2018-09-26 BR BR112020006476-9A patent/BR112020006476A2/pt not_active IP Right Cessation
- 2018-09-26 EP EP22160394.7A patent/EP4079759A1/en active Pending
- 2018-09-26 JP JP2020518670A patent/JP2020536083A/ja active Pending
-
2020
- 2020-03-15 IL IL273302A patent/IL273302A/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| EP3692062A1 (en) | 2020-08-12 |
| JP2020536083A (ja) | 2020-12-10 |
| RU2020115258A (ru) | 2021-11-08 |
| BR112020006476A2 (pt) | 2020-09-29 |
| EP4079759A1 (en) | 2022-10-26 |
| AU2018345510A1 (en) | 2020-04-09 |
| KR20200058426A (ko) | 2020-05-27 |
| US20200277329A1 (en) | 2020-09-03 |
| CA3077497A1 (en) | 2019-04-11 |
| EP3692062B1 (en) | 2022-04-13 |
| MX2020003013A (es) | 2020-07-22 |
| CN111164102A (zh) | 2020-05-15 |
| US11555052B2 (en) | 2023-01-17 |
| RU2020115258A3 (es) | 2022-01-28 |
| IL273302A (en) | 2020-04-30 |
| TW201923069A (zh) | 2019-06-16 |
| WO2019070468A1 (en) | 2019-04-11 |
| SG11202002121TA (en) | 2020-04-29 |
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