OA21090A - Nanoemulsion of 18β-Glycyrrhetinic acid. - Google Patents

Abstract

The invention refers to pharmaceutical forms that potentiate the bioavailability of 18β-GA, having a high permeation on the application area mainly due to the effect of their small particle size, high concentration of the active ingredient, excipient composition, micelles with specific structural composition and polarity. The present pharmaceutical forms exhibit specific values for particle size, permeability, diffusion coefficient, and polydispersity helping to provide its technical advantages. Therapeutic uses of the pharmaceutical forms are also described, which include those therapeutic uses for which 1 8βglycyrrhetinic acid is known to have an effect, such as anti-inflammatory, antiviral, antibacterial activity, hepatoprotective, used against skin diseases and anticarcinogenic. Mainly gelled solutions, emulsions and nanoemulsions, are intended for application in the vaginal and cervical area, for the treatment of diseases related to the human papillomavirus, such as infections, lesions, and cervical cancer.

Description

NANOEMULSION OF 18B-GLYCYRRHETINIC ACID

Field of the invention

The present invention relates to formulations and pharmaceutical forms derived 5 from a nanoemulsion containing 18p-g[ycyrrhetinic acid as a drug. Specifically, the invention provides a pharmaceutical formulation of a gelled nanoemulsion, with a concentration of glycyrrhetinic acid comprising from 0.001% to 30% w/w (percentage by weight). The different formulations are designed for the treatment of the different conditions on which the drug is known to hâve a therapeutic effect.

¹⁰ The therapeutic effects associated with the drug, and therefore with the formulations include anti-inflammatory, antiviral, antibacterial, hepatoprotective, skin diseases and anticancer activity.

The present formulations are preferably designed for the treatment of the following conditions derived from viral infections, preferably human papillomavirus 15 (HPV). HPV conditions include HPV viral infection, koîlocytes, papilloma, cervical intraepithélial lésions in their different stages, carcinomas of the genitourinary tract, perianal and rectum.

Background of the invention

Human papillomaviruses (HPV) are relatively small, non-enveloped DNA 20 viruses, of approximately 52-55 nm in diameter, which are associated with benign and malignant épithélial lésions, including >95% of cervical cancers and -20% of head and neck cancers. There are more than 200 HPV génotypes that infect and replicate in the cutaneous or mucosal epithelium, inducing benign lésions, including warts that are self-Iimiting and typically return over time.

The HPV réplication cycle and its production of virions are closely reiated to the différentiation of épithélial cells. HPVs initially infect poorly differentiated, proliférative cells and those of the stratified epithelium. Initially the viral genome takes up résidence as a nuclear plasmid with a low copy number, a subset of viral genes (the early genes) are expressed at low levels and no virions are generated.

However, the viral life cycle is initiated when the basal cells are infected and i

divided and the daughter cells migrate to the suprabasal compartment to complété différentiation.

Currently, there are physical and Chemical treatments for the control of HPV infections that produce preeancerous lésions and intraepithélial neoplasia. On the side of physical methods that use techniques aimed at eliminating the area of infection, with methods such as cryotherapy, electrocautery, cône with electrosurgery, diathermie loop, cône with CO₂ laser, CO₂ laser photovaporization and cervical cône with scalpel. As for Chemical treatments, drugs such as trichloroacetic acid, podophyllin, podophyllotoxin, 5-fluoruracîl (5-FU) and imiquimod hâve been used, which hâve been drugs proving to be effective and safe, but only in the initial treatment of anogenital warts. These treatment options hâve limited impact on cure rates and long-term survival.

Glycyrrhizic acid (GA) is a drug that has been used in some formulations for the treatment of HPV infections. It is extracted from the roots of the plant Glycyrrhiza uralensis or commonly known as licorice. This plant has been attributed antiviral, cytotoxic, antimicrobial, enzyme inhîbitory, antiinflammatory, antioxidant, and analgésie properties. There are reports describing that extracts of this plant hâve been used in ancient medicine for the treatment of diseases such as cough, asthma, lung pathologies, thorax diseases, liver diseases, intestinal disorders, stomach, indigestion, arterial diseases, diseases of the urinary System, diseases of the urinary bladder, kidney pain, expulsion of kidney stones, wounds, ulcers, granulomas, eye diseases and fever. There are also documented cases where extracts of the plant hâve been used for ulcer healing and for the treatment of spasmodic pain caused by chronic gastritîs.

It has also been documented for use as antineoplastic agent in mclanoma and gastric cancer, with anti-atherogenic activity, antioxidant effects, hypolipidémie effects, treatment of atopie dermatitis. In in vitro experiments, it has demonstrated its ability as an antiviral agent against hepatitis B virus, Epstein Barr virus, severe acute respiratory syndrome (SARS), coronavirus, Japanese encephalitis virus, human immunodeficiency virus (HIV) and herpes simplex virus.

There are some formulations on the market with GA as the active ingrédient for the treatment of HPV and related diseases. For example, the EPIGEN ® formulation is a solution containing in its composition GA in a concentration of

0.001 g/mL, labeled as an antiviral agent especially for the treatment of HPV infection, indicated for Herpes Simplex type l (labial), Herpes Simplex type 2 (génital) and Herpes Zoster infections.

Some patent applications mention formulations with GA as active ingrédient, for example, the MX Β351Π7 Patent describes a formulation composed of GA in an amount of a 0.01 to 0.2 g/mL, thermo-reversible polymer, POE-POP-POE type at 20 to 30%, trichloroacetic acid at 0.5 to 2.0%, methyl paraben and propylene glycol. Mexican application PA/E/2006/019396 mentions a formulation composed of PF-127 (20 - 30%), GA at concentrations of 0.001 to 0.5 g/mL and propylene glycol.

Although many of the pharmacological properties are attributed to GA, certain pharmacological activities are due to 18p-glycyrrhetinic acid (18β-ΟΑ), which is the aglycone resulting from the hydrolysis of GA. When the GA is administered orally there is no bioavailability of the GA, however, it is absorbed in the form of 18p-GA after its hydrolysis by intestinal bacteria. This suggests that, for the treatment of certain conditions, Ιδβ-GA is the functional active compound and not GA.

8β-glycyrrhetinic acid (Ιδβ-GA) is a pentacyclic trîterpenoîd, its molecular formula is C30H46O4. It is known by the names (2üp)-3p-hydroxy-I l-oxo-olean-12en-29-oic acid, (3β, 20P)-3-hydroxy-l 1-oxo-olean-12-en-29-oic acid, 3p-hydroxy11 -oxoolean-12-en-30-oic acid, 18p-glycyrrhetic acid, 18p-glycyrrhetinic acid, biosone, enoxolone, glycyrrhetic acid, glycyrrhetin, glycyrrhetinic acid, GM 1658, NSC 35347, olean-12-en-29-oic acid, 3-hydroxy-l 1-oxo-, (3β, 20β)-, olean-12-en30-oic acid, 3p-hydroxy-l 1-oxo-, PO 12, STX 352, subglycyrrhellic acid, uralenîc acid. There are two stereoisomers of glycyrrhetinic acid, 18α- and 18p. Although some pharmacological properties of the 18α stereoisomer are known, generally this compound is more toxic to humans, so the I8p-GA stereoisomer is the one commonly used for therapeutic purposes.

Although both GA and 18p-GA compounds are attributed with the same general pharmacological properties, e.g., anticancer, anti-inflammatory, antimicrobial, antiviral, immunoregulatory, among others, these are due to different biological and molecular mechanisms depending on the pathology in question. For example, in the case of anticancer properties, it is known that GA acts through the inhibition of enzymes such as thromboxane synthetase, HMGB1 (High-Mobility Group Box l protein) and TNF-α protein.

Likewise, it is known that GA induces apoptosis in cancer cells through the caspases and mitochondria-dependent pathway. In the case of Ιδβ-GA, it acts by decreasing the expression of proteins such as NF-κ, vascular endothélial growth factor and MMP-9 protein. Also the compound is known to induce apoptosis and cell cycle arrest in the G2 phase.

In the case of the antiviral effect, it is known that each compound acts differently for each type of virus. An antiviral effect of GA has been observed on Hepatîtis C virus (HCV) titers by preventing the release of infectious viral particles resulting in a 50% réduction, and prevents the virus from entering the cell. Demonstratîng that GA inhibits the expression of the core gene of the HCV 3A at both the mRNA and protein levels. It has been reported that GA inhibits the réplication of herpes simplex virus type l in human cells, and inhibits HIV réplication. Moreover, it inhibits herpes simplex virus (HSV) by decreasing cell adhesion, and inhibits influenza virus by preventing the interaction of viral macromolecules and proteins of the infected cell. In addition, it is known to inhibit HIV by preventing its réplication and to inhibit the H5N1 virus by controlling the proinflammatory response that it provokes and needs in order to replicate.

As for Ιδβ-GA, this compound is known to interfère with Rotavirus réplication up to 99% of infection when tested on infected cultures, by reducing the number of viral proteins VP2, VP6 and NSP2. It also inhibits HIV-1 by reducing the accumulation of viral antigen p24 and protecting cells from the cytopathological action of the virus.

These différences in mechanisms of action suggest that for a given pathology, the two drugs can hâve different mechanisms and treatment performances. For example, for the treatment of HPV infections, precancerous lésions and cervical cancer our experiments demonstrate that Ιδβ-GA has a better therapeutic effect than GA, when comparing their cytotoxic effect in different cell lines.

Therefore, the need arises to create formulations and pharmaceutical forms that hâve the drug Ιδβ-GA, for the treatment of the conditions in question. One of the limitations of Ιδβ-GA, is that it is a compound of difficult solubility in many pharmaceutically accepted solubilizing compounds, since it is insoluble in water. In addition, the compound has zéro perméation in épithélial layers which makes it diffîcult to develop an effective formulation, as would be the case for use in the treatment of infections with épithélial tissue tropism. The infections of greatest public health concern are viral infections affecting the genitourinary tract. Some of these are caused by members of the Papillomaviridae, Herpesviridae, Flaviviridae, Rotaviridae families, etc.

Therefore, a clear need arises for a pharmaceutical form capable of permeating these épithélial layers în order to hâve an effect in decreasing and/or inhibiting the disease.

The vaginal drug delivery Systems include solutions, semisolids (creams, oîntments, and gels) and solid formulations (tampons, capsules, tablets, suppositories, films, sponges, powders, and controlled-release drug delivery devices such as vaginal rings). The efficacy of these delivery Systems will dépend on their ability to achieve the approprîate local drug concentration at the site of action, their mucoadhesive properties and their compatibility with the vaginal microbiota. In order to achieve a desired concentration, it must be achieved despite the variations intrinsic to this anatomical area such as épithélial thickness, changes in the physicochemical composition of the vaginal microenvironment that are a conséquence of physiological conditions (menstruation, adolescence, infections, sociocultural habits, sexual activity, among others). On the other hand, vaginal sécrétions may reduce the bioavailability of the drug.

Given the above, it is necessary to hâve a formulation that îs effective both în concentration and in a short time interval; that is, the formulation must hâve the capacity of adéquate perméation in a short time.

For the case of 18βΌΑ, it is important to obtain a formulation whose perméation is încreased, since the I8p-GA compound alone has undesirable physicochemical properties such as poor lipophilicity, poor bioavailability, and low water solubility, which drastically decrease their percutaneous absorption profile (Hao J., et al. Int J. Pharmaceut. 399, 102-108 (2010))( Li, S., et al. Drug Dev. Ind. Pharm. 38, 855-865 (2012)). It is widely known that the pénétration of a compound in the skin dépends on the logarithm of the partition coefficient (log P), which is an indicator of the lipophilicity of a compound and its molecular weight.

This îs mainiy caused by the lipophilie properties of the outer, dead layer of the skin: the stratum corneum. Compounds with a logP of about 1 to 4 and molecular weights below 500 Da can easily penetrate through the skin, and drugs used for topical application are designed accordingly. Although the molar mass ofthe Ι8β5 GA is less than 500 Da, more precisely 470.7 Da, its logP value is quite high (6.574), therefore, a strategy to increase the permeability of 18[3-GA is needed.

One way to increase drug permeability is through formulations that constitute micro- and nano-emulsions. An émulsion is a liquid System consisting of two or more immiscible liquids, in which droplets of one liquid (the dispersed phase) are 10 dispersed in another liquid (the continuous phase), and the boundary between the two phases is known as the interface. On the other hand, micro- and nanoemulsions are émulsions with high surfactant and cosurfactant content, capable of forming dispersed, translucent liquid Systems and whose main characteristic is their reduced particle size ranging from 1 nanometer (nm) to 1 micrometer (pm).

They differ from other Systems such as nanoparticles, liposomes, or vesicles, because these are solid or semi-solid particles dispersed in a liquid or gas phase. Due to their small particle size, nanoemulsions are formulations that can penetrate through different membranes or tissues and potentiate the bioavailability of the drug or drugs that hâve reduced permeability, either topically or orally.

There are some formulations and pharmaceutical forms for the treatment of

HPV infections, precancerous lésions and cervical cancer that contain the 18p-GA as the active ingrédient. For example, application MX/a/201 7/010806 mentions a gel dosage form with a formulation comprising a POE-POP-POE-type thermoreversible gel, Ιδβ-GA, a phytoalexïn (resveratrol), a biguanide (metformin) and extract of Lactobacillus sp.

Using 18p-GA at a concentration of a 0.1 to 0.75 g/mL. In the above example, resveratrol is physicochemically unstable at room température (which reduces its half-life and compromises the shelf formulation). In addition, this formulation does not comprise a nanoemulsion, as it îs only a gelled 18p-GA solution.

S. Li et. al. fSkin Pharmacol Physiol 2012; 25:257-268) mentions obtaining a formulation consisting of a hydrogel with a liposome system of 18p-GA and lysine on concentrations of 0.3 to 0.9 % w/w and with particle sizes of 150 pm. Unlike a micro or nanoemulsion, liposomes are composed of phospholipids and are not necessarily suspended in an aqueous or oily phase.

Puglia C. et al. (Drug delivery, 2010, vol. 17, no.3, p. 123-129.) mention a nanoemulsion of 18p-GA with particle sizes of 180 to 240 nm, a concentration of 0.5%, reduced stability and a permeability of 0.60±0.08pg/h/cm2, intended for use as an anti-inflammatory.

Brief description of the invention

The present invention relates to a nanoemulsion that potentiates the bioavailability of Ιδβ-GA upon absorption at the spot or area of application, due to the effect of the composition of excipients (formula) which in combinations form nanoemulsions with polarity, structure and particle size from 1 to 500 nm, preferably from 1 to 50 nm, and from 500 to 1000 nm, preferably from 600 to 700 nm, capable of enhancing permeability, and consequently the bioavailability of the drug on the application tissue.

Such nanoemulsion can be gelled to împrove rétention at the area of application for the treatment of certain conditions, e.g., HPV skin infections, cervical intraepithélial lésions, and cervical cancer. The pharmaceutical forms of the present invention exhibit spécifie permeability values, diffusion coefficient, spécifie polydispersity which help the invention to provide its technical advantages.

The formulations and pharmaceutical forms according to the present invention are solutions, micro- and nanoemulsions having a high concentration of Ιδβ-GA, and a reduced particle size, as compared to other previously reported formulations. Although formulations hâve been reported with equal or higher concentrations of the active ingrédient or micro- and nanoemulsions with 18p-GA, the formulation of the present invention combines a high concentration of the active compound, a micro- or nano-emulsion and excipients that allow for enhanced perméation. The combination of these properties gives the present formulation superior perméation and stability properties, not previously reported in the literature.

The present invention relates to nanoemulsions with a high perméation by the combined effect of the reduced particle size, the high concentration of the active compound, the structural composition of its polar micelles and the émollient effect of its excipients, mainly adapted for its optimal application in the vaginal-cervical tract, for the treatment of various diseases related to infections, preneoplastic lésions and cervical cancer.

The present invention corresponds to formulations, preferably solutions, micro y nanoemulsions containing I8p-GA as active ingrédient, solubilizing éléments such as diethylene glycol dérivatives, propylene glycol or polyethylene glycol dérivatives, surfactant éléments such as polyoxyglycerides of capric, lauric, linoleic, oleic acid, or stearic acid triglycérides, one or more ethoxylated fatty alcohols as cosurfactants and an emollient oleaginous vehicle such as isopropyl myri state.

These formulations hâve high perméation properties due to their reduced particle size, structural composition and polarity of the micelles formed in the nanoemulsion, by modifying the structure of the skin tissue în the area of application. These formulations contain the drug in concentrations ranging from 0.001% to 30% by weight (0.0001 to 0.3 g/mL). Among the different compositions and pharmaceutical forms derived from such formulations aérosols, nebulizers, solutions, stérile solutions, foams, lyophilized products, solids, soft gelatin capsules, implants, transdermal patches, and gels are included. Of these phannaceutical forms, gels in particular are intended for use and application in the treatment of diseases related to viral infections such as human papillomavirus and thus, of cancer as well. Additionally, gels are designed to increase their absorption within the application area by mucoadhesive effect at viscositîes comprising from to 1500 to 2500 cP (centipoise) and presenting a pH of 3.8 to 6.5, preferably from 4.5 to 5.5, since their properties such as high perméation and viscosity enhance the bioavailability of the drug, thus helping the drug to hâve a better effect and in less time.

GA and 180-GA are different active ingrédients but hâve been suggested as équivalent by some authors. In this sense, a cell survival inhibition assay of different cell lines was carried out when exposed for hours 24 to one of the two drugs from Figure 1. In this experiment, four cell lines were tested; HeLa, which are cell lines représentative of cancer and HPV-18 infection, SiHa which are cells représentative of cervical cancer with HPV-I6 infection, C-33A which are cells représentative of HPV-negative cervical carcinoma, and HaCaT, which are cancer and infection négative cells. The experiment consisted of exposing cells of the different cell lines to different concentrations of both drugs for 24 hours (10-100 μΜ) going in steps of 10μΜ each. The results demonstrate that, for the cell lines représentative of HPV infection and cervical cancer, 18β-Α has overall a greater therapeutic effect compared to the effect of GA, which shows a minor effect in inhibiting cell survival (HeLa and C-33A cell lines) or no effect as in the case of the SiHa cell line. These results as a whole, suggest that for the treatment of conditions such as HPV infection and cervical cancer, Ιδβ-GA has a greater effect than GA in decreasing the survival of cancer cell lines.

This différence in drug performance can be explained by the différence in their mechanisms of action. Figure 2 shows the results of a cornet assay or single cell electrophoresis assay. The cornet assay is an experiment that allows to see if the drug has a cytotoxic effect related to DNA fragmentation of the cells. This différence is vîsualized by the size of the taiis of the cornets, which is the fragmented DNA from the cells under study. As can be seen in Figure 2A, Ιδβ-GA generates considérable DNA fragmentation in (HeLa) cancer cells treated with the drug. Comparatively, healthy (HaCaT) cells show much less fragmentation when treated with the drug. On the other hand, both cancer cells (HeLa) and healthy cells (HaCaT) treated with GA do not present this fragmentation. Figure 2B shows the graphe and statistical analysis of the results of this test. An analysis with a Wîlcoxon test was performed to verify whether the means in the percentage of DNA in the cornet taiis of the different cell lines differ. For the case ofthe HaCaT control cell line, the value of P>0.05 indicates that there is no différence between the different treatments in that cell line. In the case of the HeLa cell line, the value of P<0.05 indicates a différence in the effect of the different treatments applied to that cell line, especially Ιδβ-GA. In conclusion, these experiments make it clear that 1 δβ-GA has a sélective cytotoxic effect on cancer cells, causing DNA fragmentation by programmed cell death or 18p-induced apoptosis; unlike GA, where the aforementioned effect on the treated cells did not occur.

Brief description of the figures

Figure 1 shows the percentage of cancer cell survival and HPV infection upon exposure to GA and Ιδβ-GA drugs. It shows that Ιδβ-GA has a broader and stronger cytotoxic effect compared to GA.

Figure 2 depicts représentative tail-DNA percentage data. (A) Microphotographs of the alkaline cornet assay showing représentative images of the CC cell lines and their négative control with their 18β and GA treatments of HeLa (HPV 18 cervical cancer) and HaCaT (HPV négative immortalized kératinocyte) (B) Cornet assay data plot of Tail-DNA percentage **P<0.43 for HaCaT and P<2’¹⁶. Larger cornets for 18β treatment are observed.

Figure 3 shows the in vitro permeability test; (A) Schematic drawing of the experiment, the assembly of the skin tissue simulation membrane is shown, to test the perméation and biological effect of the different formulations. The system consists of a culture well, with cells în IxlQÔ monoiayer. Inside the well 8-10 mL of culture medium are placed and a device that has the ability to float on the medium; on the inside of the device there is a Millipore Strat-M® membrane with a pore size designed to simulate skin conditions and it is hermetically sealed. The pharmaceutical form to be tested is placed inside the flotation device with the membrane already in place. If the pharmaceutical form permeates the membrane, a greater effect on cell survival will be observed.

Figure 4 depicts the flow cytometry of the in vitro permeability assays; (A-C) Results show cell survival and cell death data from experiments performed with the setup displayed in Figure 3. Quantification of viable and nonvîable cells was determined by flow cytometry by Live/Dead Cell Double Staining Kit (Sigma) live/dead cell analysis. The results show that HeLa cervical cancer cell cultures exposed to the émulsion and nanoemulsion hâve a higher proportion of non-viable cells (41-94% non-viable cells, respectively), compared to a solution (13%). On the other hand, control cells (HaCaT) do not show a significant decrease în the proportion of viable cells when exposed to any of these three formulations (0.1- 0.3% non-viable cells). Overall, it is shown that, the higher the permeability of the active compound, the better the cytotoxic effect on cancer cells.

Figure 5 shows the graph of the particle size averages of the different formulations. The averages of the different formulations with their different particle sizes over a wide range of sizes can be observed. Emulsion: 2500 nm; Nanoemulsion l: 789.45 nm; Nanoemulsion 2: 678.13 nm; Nanoemulsion 3; 7.08 nm; Nanoemulsion 4 nm. The sizes dépend on the ratio of the excipients used in the formulation, with the size not being linearly dépendent on any spécifie component.

Figure 6 shows the graph of the average hydrodynamîc radii of the particles of the various formulations of the invention. Emulsion: 2850 nm; Nanoemulsion 1: 820 nm; Nanoemulsion 2: 750 nm; Nanoemulsion 3: 21.16 nm; Nanoemulsion 26.94 nm.

Figure 7 depicts the permeability plot obtained from the different formulations of the invention.

Figure 8 shows a linear régression between particle size and perméation of 1 8βGA from the formulation. The R2(_r squared) value close 0 and P>0.05 (p-value) indicate that there is no corrélation between particle size and perméation of Ιδβ-GA. This indicates that, for a given formulation, ail its components (particle size, type and proportion of excipients, concentration of the active ingrédient and emollient effect of the excipients) are involved in achieving higher permeability.

Detaîled description of the invention

The present invention refers to a nanoemulsion that potentiates the bioavailability of Ιδβ-GA, when absorbed on the spot or area of application to be treated by effect of its reduced particle size of 1 to 100 nm and 500 to 1000 nm, preferably at a concentration of 0.01% to 30% and a particle or micelle size of 1 to 500 nanometers, preferably from 1 to 50 nm, preferably from 1 to 20 nm in one embodiment, and from 500 to 1000 nanometers, preferably from 600 to 700 nm in another embodiment. The formulation comprises the active ingrédient glycyrrhetînîc acid (Ιδβ-GA), a solubilizing element such as diethylene glycol dérivatives, propylene glycol dérivatives, or polyethylene glycol dérivatives, a surfactant element such as a polyoxylglyceride, e.g., polyoxylglyceride from capric, lauric, linoleic, oleic acid, or of triglycérides of stearic acid, a cosurfactant such as one or more ethoxylated fatty alcohols, for example, alcohols with the structure R-(OCH2CH2)_n-OH where R is an alkyl chain with a size of 12 to 18 carbons and n represents the moles of ethylene oxide, which can hâve a value of 2 to 25 and an emollient oleaginous vehicle, e.g., isopropyl myristate. The combination of the various excipients with the drug générâtes micelles with a polar structural composition that improve the bioavailabilîty of the active ingrédient by contributing to the perméation of the formula. Lîkewise, the excipients used in the formulation help synergistically to increase perméation, due to the emollient effect of some of them.

The pharmaceutical forms in accordance with the present invention present a perméation value of 0.6 to 47.8 pg/h/cm2, wherein the composition has a diffusion coefficient of 0.003 to 1.5 pm2/_s and a polydispersity of 2 to 50.

Such formulation can be used in a liquid and packaged form in various présentations for the treatment of different diseases for which 18p-GA is known to hâve a therapeutic effect. Lîkewise, the nano-emulsion can be gelled, with the aid of certain excipients for convenient application in the treatment of certain conditions such as injuries, viral infections, and the treatment of certain cancers.

The present invention provides a sériés of formulations containing 18p-GA, in combination with other excipients suitable for each pharmaceutical formulation. In summary, the formulation îs composed of diethylene glycol dérivatives, polyoxyglycerides, an oleaginous vehicle, fatty alcohols and their dérivatives, castor oil and Silicon dioxide, as well as preservatives and stabilizers,

The formulations address the need for formulations whose active ingrédient is 18P-GA in concentrations from 0.001 to 30% by weight with high perméation ofthe active ingrédient. The different formulations are intended for its administration through various routes, such as oral, inhaled, injectable and topical.

In one embodiment of the invention the formulation is a solution of 18p-GA, comprising a solubilizing element, for example, diethylene glycol, propylene glycol or polyethylene glycols dérivatives, preferably 2-(2-ethoxyethoxy)-ethanoI, a stabilizing element, e.g., polyoxylglycerides of capric, lauric, linoleic, oleic acid, or stearic acid triglycérides, and an emollient oleaginous vehicle, e.g., isopropyl myristate. In another embodiment, in addition to the above components, the mixture can contain one or more ethoxylated fatty alcohols or their dérivatives, as well as castor oil or a derîvative thereof, such as castor oil polyoxyethylenes as stabilizing éléments.

In a preferred embodiment, the method of préparation of the solution consists of mixing the components of the formula and heatîng thîs mixture until the active ingrédient is solubilized. Once the dissolution of the active ingrédient is completed, it is cooled to room température with constant stîrring. The components of the mixture are in a w/w percentage (weight percentage) from 0.001 to 30%, preferably from 0.001 to 5% for the Ιδβ-GA, 40 to 60% for the diethylene glycol dérivative, 1 to 15% for the polyoxylglyceride, 10 to 20% for the oleaginous vehicle or emollient, 0 to 15% for the ethoxylated fatty alcohol and 0 to 20% for castor oil or its dérivatives.

In another embodiment of the invention the formulation is an émulsion, wherein the émulsion comprises a diethylene glycol dérivative, preferably 2-(2-ethoxyethoxy)ethanol, an emollient oleaginous vehicle, for example, isopropyl myristate, castor oil or a dérivative thereof and distilled water. In a preferred embodiment the method of préparation is a reverse phase inversion émulsion. In such a method the components are mixed in w/w percentages from 40% to 60% for the diethylene glycol dérivative, 10% to 20% for the oleaginous vehicle, 0% to 20% for the castor oil or its dérivatives, and 0% to 15% for the ethoxylated fatty alcohol. To these components, the active ingrédient 1 8β is added in a w/w concentration of from to 0.001 to 30%, preferably from 0.001 to 5%. The mixture is kept under stirring and heated until the active ingrédient is dissolved. Once the active ingrédient is dissolved, distilled water is added in the necessary amount, ranging from 5 to 50% by weight of the total mixture.

In another embodiment of the invention the formulation is a nano-emulsion, which is composed of a diethylene glycol dérivative, preferably 2-(2-ethoxyethoxy)ethanol, a polyoxylglyceride of capric, lauric, linoleic, oleic acid, or stearic acid triglycérides, an oleaginous vehicle or emollient, for example, isopropyl myristate and distilled water. In a preferred embodiment the method of préparation, it is a reverse phase inversion émulsion. In such a method the components are mixed in w/w percentages from 40% to 60% for the diethylene glycol dérivative, 10% to 20% for the oleaginous vehicle and 0 to 15% for the ethoxylated fatty alcohol. To these components, the active ingrédient Ιδβ-GA is added în a w/w concentration of 0.001 to 30%, preferably of 0.001 to 5%. The mixture is maintained under stirring and heated until the active ingrédient is dissolved. Once the active ingrédient is dissolved, distilled water is added in the necessary amount, ranging from 5 to 50% by weight of the total mixture with the above-described process, a nano-emulsion is obtaîned, with variable particle sizes depending on the excipients used. The increased permeability of the different formulations is achieved thanks to the combined action of the different components of the formulation. Specifically, there are three éléments that provide the increased permeability:

• The solubilizing element, in this case the diethylene glycol dérivative, referentially 2-(2-ethoxyethoxy)-ethanol helps to achieve a high solubility of the active compound Ι8β-ΘΑ and thus increases the concentration of it in the formulation and consequently, it provides a higher perméation.

• The surfactant and cosurfactant éléments, in this case fatty alcohols and their derivatives, for example alcohols with the general structure R-(OCH2CH₂)nOH where R îs an alkyl chain with a size from 12 to 18 carbons and n represents the moles of ethyiene oxide, which can hâve a value of 2 to 25, which help to give the particles their micellar structure with polarity. The amphipathic nature of these compounds helps, on the one hand, the lipophilie part of the fatty alcohols to encapsulate the diluent, in this case the diethylene glycol dérivative, e.g. 2-(2ethoxyethoxy)-ethanol, with the active compound in the micelles, and on the other hand, the polar hydrophilic part of the alcohols helps the dispersion ofthe particles or micelles in the aqueous and oily phases of the medium, preventing their agglomération due to the electrostatic repulsion between particles, thus granting stability to the formula. In addition, the polar nature of the particles helps the particles to pass through the different cellular layers that make up the tissue in the area of application.

• The emollient element of the oil phase, in this case the oleaginous vehicle, e.g., isopropyl myristate. Its activity as an emollient helps to improve the permeability of substances through the skin, as it has the effect of softening the outer layers of the skin, by moîsturizing them.

In a preferred embodiment, the particle sizes of the micro-emulsion and nanoemulsion are in the range of l nm to 500 nm (nm nanometers), preferably from I to 50 nm, preferably from l to 20 nm. In another preferred embodiment, particle sizes are in the range of from 500 to 1000 nm, preferably from 500 to 700 nm, preferably from 500 to 600 nm.

The present invention also provides a method for the préparation of solutions, émulsions and gelled nanoemulsions formulations, wherein the method comprises the steps of:

• A solution, émulsion or nano-emulsion is prepared as described in the previous steps.

• Gelling éléments and/or excipients are added.

The mixture is subjected to stirring. Preservatives and stabilîzers are added.

• The pH is adjusted according to the pharmacological application, being this setting preferably from 4 to 5 for the case of vaginal application.

• In a preferred embodiment, the excipients and gelling éléments are: cellulose dérivatives, e.g., hydroxypropyl cellulose (HPC), hydroxyethyl cellulose (HC) or hydroxypropyl methyl cellulose (HPmC), acrylic acîd polymers or a member of the poloxamer family.

• In a preferred embodiment the preservative and stabilizing agents may be: Butylated hydroxytoluene (BHT) (antioxidants), butylated hydroxyanisole, Lcysteine, propylparaben, methylparaben, benzalkonium chloride, sodium benzoate and benzoic acîd.

In certain préférable but non-limiting embodiments, the various components of the above formulations are in a non-limiting manner:

Diethylene glycol dérivative: 2-(2-ethoxyethoxy)-ethanol

Polyoxylglycerides: polyoxylglyceride of capric, lauric, linoleic, oleic acids, or stearic acid triglycérides.

Oleaginous vehicle o emollient: Isopropyl myristate.

Fatty alcohols and their dérivatives: alcohols with the general structure R(OCH2CH2)_n -OH where R is an alkyl chain with a size from 12 to 18 carbons and n represents the moles of ethylene oxide, which may hâve a value of a 2 to 25.

Anti-crystalline agent: polyvinylpyrrolidone in its different degrees of polymerization and cyclodextrins.

The following are représentative, but not limiting examples of the formulations described above:

Example 1 :

In a preferred embodiment, the invention refers to a solution with the composition:

• 18p-GA: 5.5%.

• 2-(2-ethoxyethoxy)-ethanol: 94.5%.

Which is prepared by dissolving the active compound, 18β, in 2-(2ethoxyethoxyj-ethanol, the mixture is heated in a range from 50 to 80 degrees Celsius, preterably from 60 to 70 degrees Celsius and is subjected to stirring, until dissolving the active ingrédient. The resulting solution has a perméation of 0.6 pg/h/cm2.

Example 2:

In another preferred embodiment, the invention relates to an émulsion, with a composition of:

• 18p-GA: 4.4%.

• 2-(2-ethoxyethoxy)-ethanol: 71.6%.

• Isopropyl myristate: 12%.

• Distilled water: 12%.

The method of préparation consists of mixing 2- (2-ethoxyethoxy)-ethanol and isopropyl myristate, the mixture is heated between 50 to 80 degrees Celsius, preferably from 60 to 70 degrees Celsius. Once the desired température is reached the mixture is subjected to stirring and the Ιδβ-GA is added. Once the active ingrédient is dissolved, distilled water is added to the mixture, stirring is stopped and the mixture is allowed to cool to room température. The resulting émulsion has a perméation of 1.8 pg/h/cm2, a particle size of 2500nm, a hydrodynamic radius of 2850 nm, a diffusion coefficient of 1.26 pm²/s and a polydispersity of 39.8.

Example 3:

In another preferred embodiment, the invention relates to a nano-emulsion, with a composition of:

18p-GA; 5%.

• 2-(2-ethoxyethoxy)-ethanol: 53%.

• R-(OCH₂CH₂)n-OH, where R is a 12-carbon alkyl and n is equal to 9: 2.5%.

• Hydrogenated castor oil: 6.5%.

• Isopropyl myristate: 15%.

• Distilied water: 18%.

The method of préparation consists of mixing 2-(2-ethoxyethoxy)-ethanoi, the 12-carbon ethoxylated fatty alcohol, the hydrogenated Castor Oil and the isopropyl myristate. The mixture is heated to a température of 50 to 80 degrees Celsius, preferably from 60 to 70 degrees Celsius. Once the desired température is reached, the mixture is subjected to stirring and the 18p-GA is added. Once the active ingrédient is dissolved, distilied water is added to the mixture, the stirring is stopped, and it is allowed to cool to room température. The resulting nano-emulsion has a permeability of 2.8 pg/h/cm², particle size of 789 nm, a hydrodynamic radius of 820 nm, a diffusion coefficient of 0.0038 pm²/s and a polydispersity of 26.

Example 4:

In another preferred embodiment, the invention relates to a nano-emulsion, with a composition of:

• I8p-GA: 4.5%.

• 2-(2-ethoxyethoxy)-ethanol: 54% • R-(OCH2CH2)_n-OH, where R is a 12-carbon alkyl and n is equal to 9: 3.1% • Caprylocaproyl polyoxyl-8 glyceride: 14.4%.

• Isopropyl myristate: 12%.

• Distîlled water: 12%.

The method of préparation consists of mixing the 2-(2-ethoxyethoxy)-ethanol, the 12-carbon ethoxylated fatty alcohol, the Caprylocaproyl polyoxyl-8-glycerîde and the isopropyl myristate. The mixture is heated to a temperature of 50 to 80 degrees Celsius, preferably from 60 to 70 degrees Celsius. Once the desired temperature is reached, the mixture is stirred, and the 18p-GA is added. Once the active ingrédient is dissolved, distilled water is added to the mixture, stirring is stopped and the mixture is allowed to cool to room temperature. The resulting nano-emulsion has a permeability of 8 pg/h/cm2, a particle size of 678.13 nm, a hydrodynamic radius of 750nm, a diffusion coefficient of 0.0029 gm²/s and a polydispersity of 2.3.

Example 5:

In another preferred embodiment, the invention refers to a nano-emulsion, with a composition of:

• 18P-GA: 3.9%.

• 2-(2-ethoxyethoxy)-ethanoI: 44%.

• R-(OCH2CH2)n-OH, where R is a 12-carbon alkyl and n is equal to 9: 3%.

• Caprylocaproyl polyoxyl-8 glyceride: 13%.

• R-(OCH2CH₂)_n-OH, where R is an 18-carbon alkyl and n is equal to 5: 10.6%.

• Isopropyl myristate: 18% • Distilled water: 7.5%.

The method of préparation consists of mixing the 2-(2-ethoxyethoxy)-ethanol, the 12-carbon ethoxylated fatty alcohol, the l 8-carbon ethoxylated fatty alcohol, the Caprylocaproyl polyoxyl-8 glyceride and isopropyl myristate. The mixture is heated to a température of 50 to 80 degrees Celsius, preferably from 60 to 70 degrees Celsius. Once the desired température is reached, the mixture is subjected to stirring and the Ιδβ-GA is added. Once the active ingrédient is dissolved, distilled water is added to the mixture, stirring is stopped and the mixture is allowed to cool to room température. The resulting nano-emulsion has a permeability of 47.85 pg/h/cm2, a particle size of 7.08 nm, a hydrodynamic radius of 21.16 nm, a diffusion coefficient of 0.067 μπι^/s, and a polydispersity _of 47.9, the same example sampled only 24 hours, without using stirring in the Franz cell and quantifying through HPLC coupled to UV (C 18/5pm/25cm, Z:250nm,Sodium Acetate-Tetrahydrofuran pH 4.8) obtained a recovery of 276.07 + 9.08 pg/mL (2.62 ± 0.09 mg/cm2) équivalent to 5.75 ± 0.19 pg/h/cm2 , which shows that the use of dynamic Systems (stirring) in the receiving medium of the Franz cell favors the diffusion of the drug through the membranes under évaluation, the same Systems used by several authors such as S. Li et al. Medium: PBS 7.4 Speed: 280 rpm (Skin Pharmacol Physiol 2012;25:257-268), PUGLIA C. et al. Medium: Ethanol-Water (50:50) Speed: 500 rpm (Drug delivery, 2010, vol. 17, no 3, p. 123-129.). As a physicochemical challenge to the generated nano-emulsion, it was subjected to 15 stress cycles that comprised a heating of 50°C/22.5 hours with centrifugation periods at 4000rpm/90min/4°C for a period of 15 days, obtaining no significant changes with respect to the particle size of the nanoemulsion generated.

Example 6:

In another preferred embodiment, the invention refers to a nano-emulsion, with a composition of:

* 18β-ΟΑ: 5%.

• 2-(2-ethoxyethoxy)-ethanol: 55.5%.

• Hydrogenated castor oil: 4%.

• R-(OCH₂CH2)_n-OH, where R is an 18-carbon alkyl and n is equal to 5: 15.5%.

• Isopropyl myristate: 10%.

The method of préparation consists of mixing 2-(2-ethoxyethoxy)-ethanol, hydrogenated castor oil, 18-carbon ethoxylated fatty alcohol, Caprylocaproyl polyoxyl-8 glyceride and isopropyl myristate. The mixture is heated to a température of 50 to 80 degrees Celsius, preferably 60 to 70 degrees Celsius. Once the desired température is reached, the mixture is stirred, and the 18p-GA is added. Once the active ingrédient is dissolved, distilled water is added to the mixture, stirring is stopped, and it is allowed to cool to room température. The resulting nano-emulsion has a permeability of 8 pg/h/cm2, a particle size of 7.54 nm, a hydrodynamic radius of 26.94 nm, a diffusion coefficient of 0.176 pm²/s and a polydispersity of 37.14.

Example 7:

In another preferred embodiment, the invention refers to a nano-emulsion, with a composition of:

• 18p-GA:4.0%.

• 2-(2-ethoxyethoxy)-ethanoI: 56%.

• R-(OCH₂CH₂)_n-OH, where R is a 12-carbon alkyl and n is equal to

9: 3%.

• R-(OCH₂CH₂)_n-OH, where R is an 18-carbon alkyl and n is equal to

5: 11.0%.

• Isopropyl myristate: 18%.

• Distilled water: 8.0 %.

The method of préparation consists of mixing 2-(2-ethoxyethoxy)-ethanol, the

12-carbon ethoxylated fatty alcohol, the 18-carbon ethoxylated fatty alcohol and isopropyl myristate. The mixture is heated to a température of 50 to 80 degrees

Celsius, preferably 60 to 70 degrees Celsius. Once the desired température is reached, the mixture is subjected to stirring and the Ιδβ-GA is added. Once the active ingrédient is dissolved, distilled water is added to the mixture, stirring is stopped and the mixture is allowed to cool to room température. The resulting nano-emulsion has a permeability of 2.36 ± 0.75 pg/h/cm2, a particle size of 10.35 nm, a hydrodynamic radius of 37.7 nm, a diffusion coefficient of 12.98 pm²/s and a polydispersity of 26.8, the above example dénotés that the exclusion of the Caprylocaproyl polyoxyi-S glyceride, with respect to example 5, reduces the perméation of the drug significantly (2.36 vs 47.85 pg/h/cm2), due to the change in polarity in the Nano-emulsion formed since the particles were of similar size in the two Systems (l 0.5 vs. 7.08 nm).

Example 8:

In another preferred embodiment, the invention relates to a nano-emulsion, with a composition of:

• I8p-GA: 4.3%.

• 2-(2-ethoxyethoxy)-ethanol: 47.1%.

• Caprylocaproyl polyoxyl-8 glyceride: 13%.

• R-(OCH2CH₂)_n-OH, where R is an 18-carbon alkyl and n is equal to 5: 10.1%.

• Isopropyl myristate: 18%.

• Distilled water: 7.5%.

The method of préparation consists of mixing 2-(2-ethoxyethoxy)-ethanoI, the 1 8-carbon ethoxylated fatty alcohol, the Caprylocaproyl polyoxyl-8 glyceride and the isopropyl myristate. The mixture is heated to a température of 50 to 80 degrees Celsius, preferably 60 to 70 degrees Celsius. Once the desired température is reached, the mixture is subjected to stirring and the 18p-GA is added. Once the active ingrédient is dissolved, distilled water is added to the mixture, the stirring is stopped, and it is allowed to cool to room température. The resulting nano21 émulsion has a penneability of 4.06 pg/h/cm2.

Example 9:

In another preferred embodiment, the invention refers to a nano-emulsion, with a composition of:

• 18p-GA: 4.0%.

• 2-(2-ethoxyethoxy)-ethanol: 57%.

• R-(OCH₂CH₂)_n-OH, where R is a 12-carbon alkyl and n is equal to 9: 3%.

• Caprylocaproyl polyoxyl-8 glyceride: 13%.

• R-(OCH₂CH₂)_n-OH, where R is an 18-carbon alkyl and n is equal to 5: 10.5%.

• Isopropyl myristate: 5%.

• Distilled water: 7.5%.

The method of préparation consists of mixing 2-(2-ethoxyethoxy)-ethanol, the 12-carbon ethoxylated fatty alcohol, the 18-carbon ethoxylated fatty alcohol, the Caprylocaproyl Polyoxyl-8 glyceride and the isopropyl myristate. The mixture is heated to a température of 50 to 80 degrees Celsius, preferably from 60 to 70 degrees Celsius. When the desired température is reached, the mixture is stirred, and the 18βGA is added. Once the active ingrédient is dissolved, distilled water is added to the mixture, the stirring is stopped, and the mixture is allowed to cool to room température. The resulting nano-emulsion has a penneability of 1.98 pg/h/cm2, this suggests that isopropyl myristate in proportions lower than those stated in example 5 decreases the perméation of the drug in the formed System, therefore, it is concluded that there is a dependence and synergism between the employed inputs and their proportions within the Ιδβ-GA acid nano-emulsion.

In this regard, Figure 5 shows the different particle sizes of the different formulations exemplified above. The size was determined by means of the dynamic light scattering technique, with a Litesizer equipment, previously verified with a 220 nm polystyrène latex nanoparticle suspension. Depending on the composition of the formulation, different particle sizes are obtained, rangîng, for the spécifie examples, from 7 nm in the case of the nanoemulsions to 2500 nm in the case of the émulsion. The Figure 6 shows the hydrodynamic radîi of the particles of the different formulations. This quantification was also obtained with the Litesizer equipment.

Figure 7 shows the results of the permeability analysis of the different formulations. The test was carried out by means of a permeability study of synthetic membranes in a Franz cell, where the amount of active ingrédient that passed through a membrane composed of two layers of polyethersulfone and a polyolefîn marketed under the brand name Mîllipore with polarity and pore size similar to that of human skin, and as a receptor medium, isotonie phosphate solution pH 7.4 (PBS:7.4)was quantified. As can be seen, in general, the smaller the particle size, the better the permeability of the formulation. Simîlarly, the particle size and the spécifie composition of the micelle or particles affect the permeability (the polarity of the micellar structures may favor or reduce the permeability of the System formed independently from the particle size of the structures produced).

The results of the above characterizations dénoté the importance of particle size in increasing drug perméation. However, in Figure 8, it is shown that, when making a corrélation between the particle sizes of the different formulations and their permeability, particle size is not the only factor that détermines the permeability of the formulation. This is explained by the synergistic effect between decreased particle size, the emollient effect of one of the excipients used in the formulation and the structural composition of the micelles in the formulation. The emollient effect of the excipients fulfills the function of modifying the structure of the skin, changing the stiffness of the tissue, and thereby facilitating the permeability of the nano-emulsion particles. The excipients that act as surfactants and co-surfactants give the structure of the nano-emulsion micelles a polarity, which is another factor that increases the perméation ofthe formula and helps with its stabilization, preventing the agglomération of the particles due to the electrostatîc repulsion between them.

Table l shows the comparison between different properties ofthe previously described formulations and the nano-emulsion of the present invention. The properties of the best formulation reported in each case are described in the table.

As can be seen, the present formulation has significantly smaller particle sizes compared to previously reported results. Likewise, drug perméation is significantly higher.

Table 1

Formulation	Type	%18β Concentration	Particle size (nm)	Perméation (pg/h/cm2)	Remarks
Invention	Nanoemulsion	1.0 - 5.0	7 ± 2.1	4.52 ± 0.15
Invention	Gelled Nanoemulsion	1.0 - 4.0	10.14 ± 2.5	1.66 ± 0.15
Carmelo Puglia, et al.	Nanoemulsion	0.5	210 ± 30.1	0.60 ± 0.08	Unstable at room temperature
S.Ii et al	Liposomes	0.3 - 0.9	20 to 170	0.46
Moumita Mishra et al	Nanoemulsion	Not reported	58 to 183	0.297

From the solutions, émulsions and nanoemulsions described in the present invention, following pharmaceutical forms are derived:

In one embodiment the pharmaceutical form is an aérosol. For its préparation, a nano-emulsion of 18p-GA with a particle size of 1 - 50 pm is dispersed in a pharmaceutically acceptable gas as hydrofluorocarbons hydrocarbons such as chlorofluorocarbons, hydrofluorocarbons, specifically 10 tetrafluoroethane, liquefied gases or inert gases such as nitrogen carbon dioxide, nîtrous oxide or compressed air, in doses comprising 4 pg to 20 mg per applied dose.

In one embodiment, the dosage fonn is a nebulizing agent, for its préparation, to a solution of 18p-GA dissolved in 25 - 80% ofthe final mixture, the 15 following is added: a Surfactant 5 - 30%, cosurfactant 2 - 20%, surface-active agent 5 - 30% and an oleaginous vehicle (5 - 30%) preservative 0.5 - 2%, antioxidant O.l - l.O % such as diethylene glycol monoethyl ether, polysorbate derivatives, polyoxyglycerides or myristic acid derivatives, éthanol to be used in nebulizer thérapies, by dispersing the product in isotonie solutions or in water prior to the start of treatment and obtaining particle sizes ranging from 8 nm to 1 pm.

In another embodiment, the pharmaceutical form is a stérile solution. This is prepared with glycyrrhetinic acid(I8p) dissolved in a solvent (25 - 90%), surfactant 5 - 50% and a diluent, such as diethylene glycol monoethyl ether, ethoxylated polyethylene glycol derivatives with alkylated chains from CIO to C20, Polyoxyglycerides, castor oil and its derivatives, water, éthanol, at doses of glycyrrhetinic acid(18p) which can comprise from 0.5 to 15%, the obtained solution is fîltered through a 0.22 pm membrane under aseptie conditions to proceed to terminal sterîlization by moist heat.

In another embodiment, the dosage fonn is a foam. The formulation consists of enoxolone dissolved in a solvent (25 - 90%), surfactant 5 - 50%, surface-active agent 5 - 30%, preservative 0.5 - 2%, antioxidant 0.1 - 1.0 % and a diluent, such as diethylene glycol monoethyl ether, ethoxylated polyethylene glycol derivatives with alkylated chains from CIO to C20 Polyoxyglycerides, myristic acid derivatives, castor oil and its derivatives, water, éthanol, in dosages which may comprise from 0.5 - 15 %, the above description shall be packaged with an inert gas as propellant, such gases comprise but are not limited to compressed air, nitrogen and carbon dioxide.

In another embodiment, the formulation is a lyophilized formulation. Enoxolone dissolved in a solvent (25 - 90%), Surfactant 5 - 50%, surface-active agent 5- 30%, preservative 0.5 - 2%, antioxidant 0.1 - 1.0 %, cryoprotectants 10 40 % a liquid and solid diluent, such as diethylene glycol monoethyl ether, ethoxylated polyethylene glycol derivatives with CIO to C20 alkylated chains, polyoxyglycerides, myristic acid derivatives, castor oil and its derivatives, water, éthanol, sorbitol, mannîtol, or lactose, in doses of Enoxolone that can range from 0.5 to 15 %, to be subjected to lyophilization and obtain a low water product.

In another embodiment the formulation is a solid. For its manufacture, enoxolone is mixed in pharmaceutically acceptable excipients comprising and not limited to Diluent 30 - 90%, lubricant 0.5 - 5.0%, disintegrant 0.5 - 5.0%, antisticking agent 0.5 - 5.0%, surfactants 0.5 - 10% and binders 1.0 - 20.0%; as dîluents we may mention without limiting; microcrystalline cellulose, carbohydrates and their dérivatives (lactose, mannitol, sorbitol, etc.), co-processed cellulose dérivatives and their combinations, lubricants: Sodium benzoate, stearic acid, and its dérivatives, disintegrant: sodium croscarmellose, corn starch, crospovidone, etc., Anti-sticking agent: talc, Silicon dioxide, leucine, etc. Surfactant: sodium lauryl sulfate, polysorbate and its dérivatives, docusate sodium, binder: polyvinylpyrrolidone, corn starch, cellulose dérivatives. The above mixture may be encapsulated in hard gelatin capsules or tablets by compression.

In another embodiment the formulation is a soft gelatin capsule. For its préparation, enoxolone is dissolved în a solvent (25 - 90%), Surfactant 5 - 30%, cosurfactant 2 - 20%, surface-active agent 5 - 30% and an oleaginous vehicle (5 30%), preservative 0.5 - 2%, antioxidant 0.1 - LO %, such as diethylene glycol monoethyl ether, ethoxylated dérivatives of polyethylene glycol with alkylated chains from CIO to C20, polyoxyglycerides, castor oil and its dérivatives, at dosages which may comprise from 0.5 to 15%, the above description may be dosed in soft gelatin capsules.

In another embodiment, the formulation is an implant. It is made using enoxolone dispersed in thermoplastic polymers comprising, but not limited to, polyuréthane dérivatives or polylactic acid dérivatives in proportions comprising 30 - 95 %, the above mixture may be mixed and not limited to a plasticizer, mucoadhesive for fusion molding for final application, at dosages of Enoxolone which may comprise without limitation at least 20%.

In another embodiment, the dosage form is a transdermal patch. For its préparation, enoxolone is dissolved in a solvent (25 - 90%), surfactant 5 - 30%, cosurfactant 2 - 20%, surface-active agent 5 to 30% and an oleaginous vehicle (530%), preservative 0.5 - 2%, antioxidant 0.1 to 1.0% and a diluent such as diethylene glycol monoethyl ether, ethoxylated dérivatives of polyethylene glycol with alkylated chains from CIO to C20, polyoxyglycerides, myristic acid dérivatives, castor oil and its dérivatives, water, éthanol, at dosages which may comprise from 0.5 to 20%, the above mixture is sprayed or atomized on a surface of thermoplastic polyuréthane, polyisobutylene or polylactic acid dérivatives, to form the release modulating film which will be adhered to a layer of polyethylene terephthalate which will constitute the carrier film.

Claims (18)

1. l. - Pharmaceutical formulations comprising a solution, an émulsion or a nano-emulsion of 18p-glycyrrhetinic acid, characterized in that it comprises 1 8βglycyrrhetinic acid in a concentration of 0.6% to 30% by weight of the formulation, wherein the formulation comprises a solubilizîng agent selected from diethylene glycol dérivatives, propylene glycol dérivatives or polyethylene glycol dérivatives; at least a stabilizer selected from polyoxyglycerides, castor oil or its dérivâtes or ethoxylated fatty alcohols; and an emollient oleaginous vehicle.
2. 2, - The formulations according to claim 1, characterized in that the solubilizîng agent is preferably 2-(2-ethoxyethoxy)-ethanol, wherein the stabilizer agent is preferably a polyoxylglyceride of capric, lauric, linoleic, oleic add or stearic acid triglycérides, an ethoxylated fatty alcohoï with the general structure R(OCH₂CH₂)n-OH where R is an alkyi chain with a size from 12 to 18 carbons and n represents the moles of ethylene oxide, which may hâve a value of 2 to 25; or castor oil; and the oleaginous vehicle is preferably isopropyl myristate.
3. 3, - The formulations according to claim 1, characterized in that the percentages by weight of the components mentioned in daim 2 are: 0.6% to 30%, preferably in a percentage of 0.6% to 5% of 18fi-glycyrrhetinic acid; 40% to 60% for the diethylene glycol dérivative; 1% to 15% for the polyoxylglyceride; 10% to 20% for the emollient oleaginous vehicle; 0% to 20% for the castor oil or its dérivatives and 0% to 15% for ethoxylated fatty alcohol.
4. 4. A method for preparing the formulations according to daim 1, the method is characterized in that it comprises mixing the components; heating the mixture in a range from 50 to 80 degrees Celsius, preferably from 60 to 70 degrees Celsius, adding the l8β-glycyrrhetinic acid, stirring the mixture until completely dissolved, allowing the solution to cool to room température.
5. 5. The formulations according to daim 1, characterized in that the solution has a perméation of 0.6 qg/h/cm².
6. 6. The formulation according to claim l wherein the formulation comprising I8p-glycyrrhetinic acid is an émulsion characterized în that it comprises at least an ethoxylated fatty alcohol, and distilled water; wherein the solubilizing agent îs 2-(2-ethoxyethoxy)-ethanol; the ethoxylated fatty alcohol with the general structure R-(OCH₂CH₂)_n-OH where R is an alkyl chain with size of 12 to 18 carbons and n represents the moles of ethylene oxide, which can hâve a value of 2 to 25; the oleaginous vehicle is preferably isopropyl myristate.
7. 7. The formulation according to claim 1 wherein the formulation comprising l 8p-glycyrrhetinic acid is a nano-emulsion characterized în that it comprises a polyoxylglyceride; as well as castor oil or a dérivative; and distilled water; wherein the solubilizing agent is 2-(2- ethoxyethoxy)-ethanol; a polyoxylglyceride of capric, lauric, linoleic, oleic acid, or stearic acid triglycérides; the ethoxylated fatty alcohol has the general structure R-(OCH2CH2)_n-OH where R is an alkyl chain with size from 1 2 to 18 carbons and n represents the moles of ethylene oxide, which can hâve a value from 2 to 25 or a castor oil dérivative such as polyoxyethylenes from the castor oî 1 and hydrogenated castor oils; and the emollient oleaginous vehicle is isopropyl myristate.
8. 8. The formulation according to claim 6 characterized in that the percentages by weight of the components are: 0.6% to 30%, preferably în a percentage of 0.6% to 5% of 1 8p-glycyrrhetinic acid; 40% to 60% for diethylene glycol dérivatives, propylene glycol dérivatives or polyethylene glycol dérivatives; 10% to 20% for emollient oleaginous vehicle; 0% to 20% for castor oil or dérivatives; 0% to 15% for ethoxylated fatty alcohol; and 5% to 50% for distilled water.
9. 9. A method for preparing the formulation according to claim 6, the method is characterized in that it comprises mixing the components, in percentage by weight of the components: 0.6% a 30%, preferably in a percentage of 0.6% to 5% of 18p-glycyrrhetinîc acid; 40% to 60% for diethylene glycol dérivatives, propylene glycol dérivatives or polyethylene glycol dérivatives; 10% to 20% for the emollient oleaginous vehicle; 0% to 20% for castor oil or dérivatives; 0% to 15% for the ethoxylated fatty alcohol; and 5% to 50% for the distilled water, mixing first the 2-(2-ethoxyethoxy)-ethanol and isopropyl myristate; heating the mixture in the range from 50 to 80 degrees Celsius, preferably from 60 to 70 degrees Celsius; after reaching the desired température, stirring the mixture and adding the I8p-glycyrrhetinic acid until completely dissolved, adding distilied water, stopping stirring and letting it cool down to room température.
10. 10. The formulation according to claim 6, characterized in that the resulting émulsion has a perméation of 1.8 pg/h/cm², a particle size of 2500 nm, a hydrodynamic radius of 2850 nm, a diffusion coefficient of 1.26 pm²/s and a polydispersity of 39.8.
11. 11. A gel characterized in that it comprises the formulations according to claims 1, 6 or 7, wherein the gel comprises gelling éléments such as cellulose derivatives, selected from group of hydroxypropyl cellulose (HPC), hydroxyethyl cellulose (HC) or hydroxypropyl methyl cellulose (HPmC), acrylic acid polymers or a member of the poloxamer family.
12. 12. The gel according to claim 1 1, characterized in that the gel comprises preservative and stabilizing agents selected from a group comprising butylated hydroxytoluene (BHT), butylated hydroxyanisole, L-cysteine, propylparaben, methylparaben, benzalkonium chloride, sodium benzoate and benzoic acid.
13. 13. The formulation according to claim 7, characterized in that the percentages by weight of its components are: 0.6% to 30%, preferably in a percentage of 0.6% to 5% of 1 8p-glycyrrhetinic acid; 40% to 60% for diethylene glycol derivatives, propylene glycol derivatives or polyethylene glycol derivatives; 10% to 20% for emollient oleaginous vehicle; 0% to 20% for castor oil or derivatives; 0% to 15% for ethoxylated fatty alcohol; and 5% to 50% for distilied water.
14. 14. A method for preparmg the formulation according to claim 7, the method is characterized in that it comprises mixing the components in percentage by weight of the components: 0.6% to 30%, preferably in a percentage of 0.6% to 5% of 18p-glycyrrhetinic acid; 40% to 60% for diethylene glycol derivatives, propylene glycol derivatives or polyethylene glycol derivatives; 10% to 20% for the emollient oleaginous vehicle; 0% to 20% for castor oil or derivatives; 0% to
15. 15% for ethoxylated fatty alcohol; and 5% to 50% for distilied water, mixing first the diethylene glycol dérivative, propylene glycol derivatives or polyethylene glycol derivatives, the emollient oleaginous vehicle, the castor oil or derivatives and the ethoxylated fatty alcohol; heating the mixture in the range of 50 to 80 degrees Celsius, preferably from 60 to 70 degrees Celsius; stirring the mixture and adding 18p-glycyrrhetinic acid until completely dissolved, adding distilled water, stopping stirring and allowing to cool to room température.

    5 15. The formulation according to claim 7, characterized in that it has a particle size of l to i 00 nm, preferably l to 20 nm, or from 500 to I000 nm, preferably 600 to 700 nm.
16. 16. The formulation according to claim 7, characterized in that the perméation value of the nano-emulsion is of 0.6 to 47.85 pg/h/cm², wherein the 10 nano-emulsion has a diffusion coefficient of a 0.003 to 1.5 pm²/s and a polydispersity of 2 to 50.
17. 17. The formulations and gels according to any precedent claim, to be used in the treatment of viral infections, wherein the use as an antiviral drug is preferably for the treatment of conditions caused by human papillomavirus, such 15 as low-grade and high-grade intraepithélial lésions and cervical cancer.
18. 18. The formulations and gels according to any precedent claim, to be used in the treatment of cancer, wherein the use as an anticancer agent is preferably for the treatment of cervical cancer.