OA20958A

OA20958A - Anti- MUC1-sea antibodies

Info

Publication number: OA20958A
Application number: OA1202200311
Authority: OA
Inventors: Daniel Rubinstein; Daniel Wreschner
Original assignee: Biomodifying, Llc; Ramot At Tel-Aviv University Ltd
Priority date: 2020-03-18
Filing date: 2021-03-11
Publication date: 2023-07-24

Abstract

The present invention provides isolated monoclonal antibodies which bind to the MUC1 SEA domain. The invention also concerns the use of these antibodies in therapeutic and diagnostic methods.

Description

ANTI-/WÜC7-SEA ANTIBODIES

TECHNOLOGICAL FIELD

The présent invention relates to antibodies specifically directed against the junction ofthe alpha and beta chains (which comprises the SEA domain) of MUC1, a glycoprotein présent on the cell surface of human cells and to methods and uses thereof in the détection and treatment of cancer, as well as in the détection and treatment of a variety of non-malignant diseases and disorders.

BAC KG ROUND ART

References considered to be relevant as background to the presently disclosed subject matter are listed below:

[1] G. Rivalland, B. Loveland, P. Mitchell, Update on Mucin-1 immunotherapy in cancer: a clinical perspective, Expert Opin Biol Ther, 15 (2015) 1773-1787.

[2] J. Taylor-Papadimitriou, J.M, Burchell, R. Graham, R. Beatson, Latest developments in MUC1 immunotherapy, Biochem SocTrans, 46 (2018) 659-668.

[3] J.M. Burchell, A. Mungul, J. Taylor-Papadimitriou, O-linked glycosylation in the mammary gland: changes that occur during malignancy, J Mammary Gland Biol Neoplasia, 6 (2001) 355-364.

[4] W. Fiedler, S. DeDosso, S. Cresta, J. Weidmann, A. Tessari, M. Salzberg, B. Dietrich, H. Baumeister, S. Goletz, L. Gianni, C. Sessa, A phase I study of PankoMab-GEX, a humanised glyco-optimised monoclonal antibody to a novel tumour-specîfic MUC1 glycopeptide epitope in patients with advanced carcinomas, Eur J Cancer, 63 (2016) 5563.

[5] K. Ryuko, D.J. Schol, F.G. Snijdewint, S. von Mensdorff-Pouilly, R.J. Poort-Keesom, Y.A. Karuntu-Wanamarta, R.A. Verstraeten, K. Miyazaki, P. Kenemans, J. Hilgers, Characterization of a new MUC1 monoclonal antibody (VU-2-G7) directed to the glycosylated PDTR sequence of MUC1, Tumour Biol, 21 (2000) 197-210.

[6] M.A. Tarp, A.L. Sorensen, U. Mandel, H. Paulsen, J. Burchell, J. Taylor-Papadimitriou, H. Clausen, Identification of a novel cancer-specific immunodominant glycopeptide epitope in the MUC1 tandem repeat, Glycobiology, 17 (2007) 197-209.

[7] D. Zhou, L. Xu, W. Huang, T. Tonn, Epitopes of MUC1 Tandem Repeats in Cancer as Revealed by Antibody Crystallography: Toward Glycopeptide Signature-Guided Therapy,

Molécules, 23 (6) (2018) 1326.

[8] T. Kimura, O.J. Finn, MUC1 immunotherapy is here to stay, Expert Opin Biol Ther, 13 (2013) 35-49.

[9] N,K. Ibrahim, K.O. Yariz, I. Bondarenko, A. Manikhas, V. Semiglazov, A. Alyasova, V. Komisarenko, Y. Shparyk, J.L. Murray, D. Jones, S. Senderovich, A. Chau, F. Erlandsson, G, Acton, M. Pegram, Randomized phase II trial of letrozole plus anti-MUC1 antibody AS 1402 in hormone receptor-positive locally advanced or metastatic breast cancer, Clin Cancer Res, 17 (2011) 6822-6830.

[10] D.B. Rubinstein, M. Karmely, R. Ziv, I. Benhar, O. Leitner, S, Baron, B.Z. Katz, D.H. Wreschner, MUC1/X protein immunization enhances cDNA immunîzation in generating anti-MUC1 alpha/beta jonction antibodies that target malignant cells, Cancer Res, 66 (2006) 11247-11253.

[11] E. Pichinuk, I. Benhar, O. Jacobi, M. Chalik, L. Weiss, R. Ziv, C. Sympson, A. Karwa, N.l. Smorodinsky, D.B. Rubinstein, D.H. Wreschner, Antibody targeting of cell-bound MUC1 SEA domain kills tumor cells, Cancer Res, 72 (2012) 3324-3336.

[12] D.B. Rubinstein, M. Karmely, E. Pichinuk, R. Ziv, I. Benhar, N. Feng, N.l. Smorodinsky, D.H. Wreschner, The MUC1 oncoprotein as a functional target: immunotoxin binding to alpha/beta junction médiates cell killing, Int J Cancer, 124 (2009) 46-54.

[13] D.V. Gold, Z. Karanjawala, D.E. Modrak, D.M. Goldenberg, R.H. Hruban, PAM4reactive MUC1 is a biornarker for early pancreatic adenocarcinoma, Clin Cancer Res, 13 (2007) 7380-7387.

Acknowledgement of the above references herein is not to be inferred as meaning that these are in any way relevantto the patentability ofthe presentiy disclosed subject matter.

BACKGROUND

The MUC1 glycoprotein is over-expressed by a variety of high-incidence, high-mortality human épithélial malignancies, including breast, prostate, pancréas, ovarian, lung, and colon carcinomas, as well as by the malignant plasma cells of multiple myeloma, and by the myelogenous cells of acute myelogenous leukemia. Because of its preferentially high expression by malignant cells, and because it is expressed on the cell surface and therefore is an exposed molécule, MUC1 has been studied as both a target for directed cancer .( therapy and as a marker of disease progression [1, 2],

Structurally, the MUC1 molécule îs a transmembrane glycoprotein (termed MUC-TM). MUC-TM is a heterodimer consisting of an extracelluiar domain containing between 20 to 125 repeats of a 20 amino acid-long sequence (termed the variable number tandem repeat, VNTR), a transmembrane domain, and a short cytoplasmic tail mediating intracellular signaling (see Figure 1A). The MUC1 molécule is auto-proteolytically cleaved within the SEA module, a highly-conserved domain of 120 amino acids. This results in a large extracellular a subunit containing the tandem repeat array bound in a strong non-covalent interaction to a transmembrane β subunit containing the transmembrane and cytoplasmic domains ofthe molécule. Binding ofthe a chain to the β chain is intermittent: The a chain binds the β chain in an on-and-off manner. While the β chain remains on the cell surface ail the time, the a chain with its VNTR remains cell-bound only intermittently.

A number of anti-MUC1 antibodies hâve been reported in the literature with the great majority directed against the highly immunogenic VNTR of the a chain. While anti-VNTR antibodies can successfully bind MUC1+ cells in vitro, the shedding ofthe MUC1 a chain containing the VNTR into the peripheral circulation in vivo severely compromises the ability of anti-VNTR antibodies to clinically affect MUC1 expressing tumors. Shedding of the a chain off the tumor cell surface not only greatly decreases the number of MUC1 targets for anti-α chain antibodies, but additionally, freely circulating MUC1 a chain in vivo in the periphery can bind and neutralize anti-VNTR antibodies or anti-glycosylated-VNTR antibodies thereby limiting their ability to even reach MUC1 -expressing tumor.

Antibodies that recognize cancer-specifîc truncated O-glycoforms of the VNTR, such as the antibodies PankoMab-Gex, 5E5, SM3, and VU-2-G7, hâve been proposed as possible ways to overcome the potential toxicity of targeting MUC1 expressed by normal tissues. However, the limitations of targeting the α-chain VNTR (Fig 1A), namely its shedding from the cell surface and its ability to bind therapeutically administered anti-MUC1 antibody, remain [3-7],

Because of the instabilïty of their target, which, as noted, binds the tumor cell only intermittently in an on-and off mechanism, no anti-MUC1 VNTR antibody has yet been proven to be clinically effective [4, 8, 9], Of note, Fiedler et al reported 16 cases of stable disease in a clinical trial of anti-VNTR cancer-specifîc glycosylation antibody PankoMabGex [4], However, that study was a Phase I trial of the anti-VNTR antibody, where the primary study objective was antibody safety rather than anti-tumor efficacy, such that the cases of stable disease observed cannot be truly interpreted. To date, no antibodies against the MUC1 VNTR, nor any antibodies against the a chain of MUC1 has ever been shown to be effective against tumors in hum ans.

In contrast to the a Chain and its VNTR, the MUC1 SEA domain formed by the interaction of the α-subunit with the extracelluiar portion of the β-subunit îs a stable cell membranefixed molecular moiety. Anti-MUC1 alpha/beta junction antibodies were disclosed in Rubinstein et al and in Pichinuk et al [10-12],

GENERAL DESCRIPTION

In a first aspect, the présent invention provides an isolated monoclonal antibody or antigenbinding fragment thereof which binds to the MUC1 SEA domain, wherein said antibody comprises:

a. a heavy chain complementarity determining région (CDRH) 1 denoted by SEQ ID NO. 25, CDRH2 denoted by SEQ ID NO. 26, CDRH3 denoted by SEQ ID NO. 27, and a light chain complementarity determining région (CDRL) 1 denoted by SEQ ID NO. 28, a CDRL2 denoted by SEQ ID NO. 29, and a CDRL3 denoted by SEQ ID NO. 30; or

b. a heavy chain complementarity determining région (CDRH) 1 denoted by SEQ ID NO. 31, CDRH2 denoted by SEQ ID NO. 32, CDRH3 denoted by SEQ ID NO. 33, and the light chain complementarity determining région (CDRL) 1 denoted by SEQ ID NO. 34, a CDRL2 denoted by SEQ ID NO. 35, and a CDRL3 denoted by SEQ ID NO. 36; or

c. a heavy chain complementarity determining région (CDRH) 1 denoted by SEQ ID NO. 37, CDRH2 denoted by SEQ ID NO. 38, CDRH3 denoted by SEQ ID NO. 39, and the light chain complementarity determining région (CDRL) 1 denoted by SEQ ID NO. 40, a CDRL2 denoted by SEQ ID NO. 41, and a CDRL3 denoted by SEQ ID NO. 42; or

d. a heavy chain complementarity determining région (CDRH) 1 denoted by SEQ ID NO. 43, CDRH2 denoted by SEQ ID NO. 44, CDRH3 denoted by SEQ ID NO. 45, and the light chain complementarity determining région (CDRL) 1 denoted by SEQ ID NO. 46, a CDRL2 denoted by SEQ ID NO. 47, and a CDRL3 denoted by SEQ ID NO. 48; or

e. a heavy chain complementarity determining région (CDRH) 1 denoted by SEQ ID NO. 49, CDRH2 denoted by SEQ ID NO. 50, CDRH3 denoted by SEQ ID NO. 51, and the light chain complementarity determining région (CDRL) 1 denoted by SEQ ID NO. 52, a CDRL2 denoted by SEQ ID NO. 53, and a CDRL3 denoted by SEQ ID NO. 54; or

f. a heavy chain complementarity determining région (CDRH) 1 denoted by SEQ ID NO. 55, CDRH2 denoted by SEQ ID NO. 56, CDRH3 denoted by SEQ ID NO. 57, and the light chain complementarity determining région (CDRL) 1 denoted by SEQ ID NO. 58, a CDRL2 denoted by SEQ ID NO. 59, and a CDRL3 denoted by SEQ ID NO. 60.

In some embodiments, said antibody comprises a heavy Chain variable région and a light chain variable région, wherein:

a. said heavy chain variable région is encoded by a nucleic acid sequence which is at least 70% identical to the nucleic acid sequence denoted by SEQ ID NO. 1 and wherein said light chain variable région is encoded by a nucleic acid sequence which is at least 70% identical to SEQ ID NO. 2; or

b. said heavy chain variable région is encoded by a nucleic acid sequence which is at least 70% identical to the nucleic acid sequence denoted by SEQ ID NO. 3 and wherein said light chain variable région is encoded by a nucleic acid sequence which is at least 70% identical to SEQ ID NO. 4; or

c. said heavy chain variable région is encoded by a nucleic acid sequence which is at least 70% identical to the nucleic acid sequence denoted by SEQ ID NO. 5 and wherein said light chain variable région is encoded by a nucleic acid sequence which is at least 70% identical to SEQ ID NO. 6; or

d. said heavy chain variable région is encoded by a nucleic acid sequence which is at least 70% identical to the nucleic acid sequence denoted by SEQ ID NO. 7 and wherein said light chain variable région is encoded by a nucleic acid sequence which is at least 70% identical to SEQ ID NO. 8; or

e. said heavy chain variable région is encoded by a nucleic acid sequence which is at least 70% identical to the nucleic acid sequence denoted by SEQ ID NO. 9 and wherein said light chain variable région is encoded by a nucleic acid sequence which is at least 70% identical to SEQ ID NO. 10; or

f. said heavy chain variable région is encoded by a nucleic acid sequence which is at least 70% identical to the nucleic acid sequence denoted by SEQ ID NO. 11 and wherein said light chain variable région is encoded by a nucleic acid sequence which is at least 70% identical to SEQ ID NO. 12.

In some embodiments, said antibody comprises:

a. a heavy chain variable région comprising the amino acid sequence denoted by SEQ ID NO. 13 or a variant thereof and a light chain variable région comprising the amino acid sequence denoted by SEQ ID NO. 14, or a variant thereof; or

b. a heavy chain variable région comprising the amino acid sequence denoted by SEQ ID NO. 15 or a variant thereof and a light chain variable région comprising the amino acid sequence denoted by SEQ ID NO. 16, or a variant thereof; or

c. a heavy chain variable région comprising the amino acid sequence denoted by SEQ ID NO. 17 or a variant thereof and a light chain variable région comprising the amino acid sequence denoted by SEQ ID NO. 18, or a variant thereof; or

d. a heavy chain variable région comprising the amino acid sequence denoted by SEQ ID NO. 19 or a variant thereof and a light chain variable région comprising the amino acid sequence denoted by SEQ ID NO. 20, or a variant thereof; or

e. a heavy chain variable région comprising the amino acid sequence denoted by SEQ ID NO, 21 or a variant thereof and a light chain variable région comprising the amino acid sequence denoted by SEQ ID NO, 22, or a variant thereof; or

f. a heavy chain variable région comprising the amino acid sequence denoted by SEQ ID NO. 23 or a variant thereof and a light chain variable région comprising the amino acid sequence denoted by SEQ ID NO. 24, or a variant thereof.

In some embodiments, said antibody is a complété mouse antibody, a complété chimeric antibody, a complété humanized antibody (e.g., wherein parts of the antibody sequence are derived from mouse sequence and part from human), or a complété human antibody.

In some embodiments, said antigen-binding fragment thereof is Fv, single chain Fv (scFv), single chain Fv-Fc (scFv-Fc), Fab’, Fab, F(ab’)₂ or F(ab)₂.

In some embodiments, said antibody or antigen-binding fragment thereof identify MUC1 SEA in immunohistochemistry performed on formaldéhyde fixed sections from Fresh Frozen (FF) tissues, and/or on Paraffin Embedded and Formaldéhyde Fixed (PEFF) tissues, and/or by fluorescence-actîvated cell sorting (FACS) analysis of MUC1-expressing human cells.

In another embodiment, the present invention provides an isolated nucleic acid molécule comprising a nucieotide sequence encoding the antibody of the invention as described herein or any antigen-binding fragment thereof.

In another embodiment, the present invention provides an expression vector comprising the isolated nucleic acid molécule of the invention.

In another embodiment, the present invention provides a host cell transfected with the expression vector of the invention.

In another embodiment, the present invention provides an immunoconjugate comprising the antibody of the invention or antigen-binding fragment thereof and an additional cytotoxic or therapeutic agent.

In certain embodiments, said cytotoxic agent is selected from a group consisting of alkylating drugs, anthracyclines, pyrimidine dérivatives, vinca alkaloids, photodynamic drugs, platinum-containing compounds, taxanes, topoisomerase inhibitors, ribosome inactivating agents, agents that induce DNA damage, tubulin inhibitors, anti-mitotic agents, radioisotopes, cytotoxic antibodies, and bacterial toxins.

In one embodiment, said cytotoxic agent is pseudomonas exotoxin.

In one embodiment, said immunoconjugate reduces tumor volume upon administration to a subject with cancer.

In another embodiment, the present invention provides a bispecific antibody comprising the antibody of the invention or antigen-binding fragment thereof bound to a second antibody binding a different antigen target.

In another embodiment, the présent invention provides a pharmaceutical composition comprising as an active ingrédient the isolated monoclonal antibody or antigen-binding fragment thereof of the invention, or the immunoconjugate of the invention, or the bispecific antibody of the invention and a pharmaceutically acceptable carrier, excipient, or diluent.

In an embodiment, said pharmaceutical composition is for use in the treatment of a disease or disorder, e.g., cancer.

In an embodiment, said pharmaceutical composition further comprises an additional therapeutic agent.

In some embodiments, the présent invention provides, a method of treatment or amelioration of a disease or disorder comprising administering to a subject in need thereof a therapeutically effective amount of at least one isolated monoclonal antibody or antigenbinding fragment thereof, or the immunoconjugate, or the bispecific antibody, or the pharmaceutical composition of the invention.

In an embodiment, said method further comprises administering to a subject in need thereof an additional therapeutic agent.

In one embodiment said disease or disorder is cancer.

In one embodiment, said cancer is a MUC1 expressing cancer.

In some embodiments, said cancer is selected from the group consisting of lung carcinoma, prostate carcinoma, breast carcinoma, ovarian carcinoma, colon carcinoma, pancreatic carcinoma, multiple myeloma, and acute myelogenous leukemia.

Ail the recited cancer types are known to express MUC1 on their malignant cells.

In one embodiment said disease or disorder is an autoimmune or inflammatory disease.

In some embodiments, said autoimmune or inflammatory disease is selected from a group consisting of, but not limited to, rheumatoid arthritis, psoriafic arthritis, systemic lupus erythematûsus, amyloidosis, and autoimmune pancreatitis.

In one embodiment said disease or disorder is a non-malignant but abnormal and clinically significant growth condition, e.g., cysts, for example rénal cysts, thyroid cysts and thyroid masses, or hepatic cysts.

In some embodiments, the présent invention provides the isolated monoclonai antibody or antigen-binding fragment thereof, or the immunoconjugate, or the bispecific antibody, or the pharmaceutical composition of the invention for use in a method of treatment or amelioration of a disease or disorder, said method comprising administering to a subject in need thereof a therapeutically effective amount of said isolated monoclonal antibody or antigen-binding fragment thereof, said immunoconjugate, said bispecific antibody, or said pharmaceutical composition.

In one embodiment, said method further comprises administering to a subject in need thereof an additional therapeutic agent.

!n one embodiment said disease or disorder is cancer.

In one embodiment, said cancer is a MUC1 expressing cancer.

In one embodiment said disease or disorder is an autoimmune or an inflammatory disease. In one embodiment said disease or disorder is a non-malignant abnormal growth condition, e.g., cysts, for example rénal cysts, thyroid cysts and thyroid masses, or hepatic cysts.

In another aspect, the present invention provides a method of diagnosing a disease or disorder in a subject, wherein said disease or disorder is associated with MUC-1 expression, said method comprising:

a. contacting a biopsy obtained from said patient with at least one isolated monoclonal antibody or antigen-binding fragment thereof of the invention; and

b. detecting said isolated monoclonal antibody or any antigen-binding fragment thereof;

wherein détection of cells over-expressing MUC1 SEA in the biopsy indicates that the subject is diagnosed with a disease or disorder.

In one embodiment said disease or disorder is cancer.

In one embodiment said disease or disorder is an autoimmune or an inflammatory disease. In one embodiment said disease or disorder is a non-malignant, clinically significant, abnormal growth condition, e.g., cysts, for example rénal cysts, thyroid cysts and thyroid masses, or hepatic cysts.

In one embodiment, said isolated monoclonal antibody or antigen-binding fragment thereof is detectably labelled.

In another aspect, the present invention provides a method of imaging a disease or disorder, said method comprising:

a. introducing at least one isolated anti-MUC1 SEA monoclonal antibody or antigenbinding fragment thereof of the invention into a subject, wherein said antibody or antigenbinding fragment thereof are detectably labelled with a radioisotope, or with a visualizable agent (i.e., an agent that can be visualized, for example by scanning); and

b. visualizing said detectably labelled isolated anti-MUC1 SEA monoclonal antibody or any antigen-binding fragment thereof;

wherein détection of cells and/or tissues labelled with said isotope or with said visualizable agent indicates the presence, and/or the localization and/or the extent and/or the presence of métastasés of said disease or disorder in said subject.

In one embodiment said disease or disorder is cancer.

In one embodiment said disease or disorder is a non-malignant abnormal growth condition, e.g., cysts, for example rénal cysts, thyroid cysts and thyroid masses, or hepatic cysfs.

BRIEF DESCRIPTION OF THE DRAWINGS

To better understand the subject matter that is disclosed herein and to exemplify how it may be carried out in practice, embodiments will now be described by way of non-limiting example only, with reference to the accompanying drawings:

Figures 1A-1C are schematic représentations of the MUC1-TM, the MUC1-X isoform, and recombinant MUC1-X molécules. (1 A) Going from the N (N) to the C terminus (C), MUC1TM is composed of an N-terminal Signal Peptide, followed by a 30 amino acid-long segment (N30), leading into sequences that N-terminaiiy and C-terminally flank the variable tandem repeat array (VNTR). This is followed by a région common to both the MUC1-X isoform (1B) and to the soluble extracellular domain of MUC1 X, MUC1-Xex (1C). The βsubunit extracellular domain consists of 58 amino acids immediately N-terminal to the transmembrane (TM) and cytoplasmic (CT) domains. The SEA module comprises 120 amino acids, contnbuted by both the a- and β-subunits. Recombinant soluble MUC1-Xex protein (1C) includes the signal peptide, the N-terminal 30 amino acid sequence and the SEA module régions.

Figures 1D-1K are graphs showing flow cytométrie analyses of the anti-MUC1 SEA antibody DMB5F3. DA3 cells stably transfected with MUC1-TM (1D) or non-transfected DA3 cells (1E) were reacted with anti-MUC1 SEA mAb DMB5F3, followed by an FITCconjugate (tracings marked by arrow). MUC1+ human pancreatic cancer cell line Colo357 (1F) and MUC1+ breast cancer cells T47D andZR75 (1H, 1J) were reacted with DMB5F3 (tracings marked by arrow). In ail four panels, the tracings not marked by arrow represent cells bound to secondary antibody alone, and the tracings marked by arrow that of cells reacted with both DMB5F3 and secondary antibody. Anti-MUC1 SEA binding to MUC1 + cells was entirely competed-out by the presence of soluble MUC1-Xex protein (MUC1-Xexmediated abrogated binding is indicated by empty arrows in 1G, 11, and 1K).

Figure 2 shows the amino acid sequences of the variable régions of the anti-MUC1 SEA α-β junction monoclonal antibodies. For ail sequences, the following general structure is presented: Leader sequence-FR1-CDR1-FR2-CDR2-FR3-CDR3-FR4.

Figures 3A-3N show the architecture of MUC1 expression delineated by the anti-MUC1 SEA α-β junction antibody DMB5F3. Figures 3A-3D: Paraffin-embedded microarrays of normal (3A and 3B) and malignant pancreatic tissue (3C and 3D) were stained with DMB5F3. DMB5F3 strongly stained malignant cells in a near-circumferential pattern (3C and 3D), while in normal pancreatic acinar cells only weak apical positrvity was seen (3A(i), black arrows). Addition of MUC1-Xex protein together with DMB5F3 competitively abrogated staining (3B), indicating the specificity of DMB5F3 binding. Figures 3E-3H: Paraffin-embedded normal breast tissues (3E and 3F) and malignant invasive ductal breast adenocarcinomas (3G and 3H) from 4 patients were stained with the DMB5F3 antibody. Figures 3I-3N Breast carcinome biopsy specimens of 6 patients, each consisting of tumor surrounded by adjacent non-malignant tissue, stained with DMB5F3. In each specimen DMB5F3 strongly stained invasive cancer épithélial cells in a near-circumferential pattern (dark staining). In confrast, adjacent normal glandular épithélial cells showed only weak apical positivity (black arrows).

Figures 4A-4O show IHC staining of tissue microarrays with anti-MUC1 SEA α-β junction antibody:

Figures 4A-4L· IHC of lung, prostate, colon, and breast carcinoma with anti-MUCI SEA α-β Junction antibody. Paraffin embedded microarrays of lung, prostate, colon, and breast carcinomas were stained with antibody DMB5F3 and représentative sections are shown in Figures 4A, 4D, 4G and 4J, respectively. Higher magnification is shown in Figures 4B, 4E, 4H and 4K and triangles in Figures 4A, 4D, 4G and 4J and in 4B, 4E, 4H and 4K serve to orientale the magnified région. Staining with antibody DMB5F3 was again abolished in the presence of 100pg/ml of competing soluble MUC1-Xex protein, attesting to the specificity of antibody DMB5F3 (not shown). Control staining with non-specific mouse immunoglobulin is shown in Figures 4C, 4F, 4I and 4L. Antibody DMB5F3 strongly stained malignant cells in a near-circumferential pattern. Lines at the bottom of Figures 4A, 4D, 4G and 4J (with arrows at either ends of the line) and at the bottom of Figures 4B, 4E, 4H and 4K (with full circles at either ends of the line) indicate 200 microns and 100 microns, respectively. Dark staining precipîtate indicates MUC1 protein (compare Figures 4A, 4B, 4D, 4E, 4G, 4H, 4J, and 4K with Figures 4C, 4F, 4I and 4L). Tissues in Figures 4A, 4D, 4G and 4J dérivé from Pathologie Grade 2 lung adenocarcinoma, Pathologie Grade 5 prostate adenocarcinoma, Pathologie Grade 3 colon adenocarcinoma, and infiltrating ductal breast carcinoma, respectively.

Figures 4M-4O: Corroborative IHC staining of normal and malignant pancreatic tissues with anti~MUC1 SEA α-β junction antibody. To corroborate high expression and altered molecular architecture of MUC1 in pancreatic carcinoma, additional pancreatic malignancies were stained with anti-MUC1 SEA and compared to normal tissue. Paraffinembedded microarrays of normal (Figure 4M) and malignant pancreatic tissue (Figures 4N and 40) were stained with antibody DMB5F3. Staining confirmed weak, apical positivity in normal pancreatic acinar cells (Figure 4M), whereas malignant cells consistently stained in a near-circumferential pattern (Figures 4N and 40).

Figures 5A-5D are graphs showing comparative cytocidal activity of chDMB5F3, Erbitux, and Herceptin, where each of the three was linked by protein ZZ to the Pseudomonas exotoxi η PE38. The three résultant 2Z-PE38 immunotoxins were reacted with human tumor cell lines T47D, KB, A431 and N87 (Fig. 5A-5D, respectively). Immunotoxin chDMB5F3: ZZ-PE38 (oval tracing), Erbitux: ZZ-PE38 (diamond tracing), and Herceptin: ZZ-PE38 (rectangular tracing) were reacted with cells at varying antibody concentrations (x-axis). Cell viability was assessed by the alkaline phosphatase assay (y-axis). Total (100%) viability was determined in control wells to which ZZ-PE38 toxin (5nM) alone had been added.

Figures 6A-6D show in vivo cytotoxicity of chDMB5F3: ZZ-PE38 in human pancreatic tumor xenotransplanted into nude mice. Figure 6A: Nude mîce were inoculated with MUC1⁺pancreatic tumor Colo357 (Day 0). The xenotransplanted mice were then divided into three groups: On Days 1, 6, 9, 14, 22 and 29 Group A received chDMB5F3: ZZ-PE38, Group B received non-specific, isotype-matched IgG-ZZ: PE38 (5pg per injection), while Group C received only Hepes buffer; ail administrations were iv (time points indicated by arrows). Tumor volume was measured weekly during the injection period and up to Day 38 in the three groups: mice receiving chDMB5F3: ZZ-PE38 immunotoxin, mice receiving nonspecific isotype-matched hlgG-ZZ: PE38, and mice receiving Hepes buffer. Tumor volume in mm³ appears on the y-axis, while photo images show the tumors in the Hepes control mice (Figure 6B) and in mice receiving chDMB5F3: ZZ-PE38 immunotoxin (Figure 6C). Figure 6D: To quantitate sérum half-life of the anti-MUC SEA DMB5F3-ZZ-P38 immunotoxin, a single 5 microgram dose was administered to mice, and sérum levels were measured by serially diluted Elisa. Compared with Day 7 (7d), serum levels of chDMB5F3 on Days 14 (14d) and 28 (28d) weretwofold and fourfold decreased, respectively.

Figures 7A-7D show cytotoxicity of chDMB5F3: ZZ-PE38 immunotoxin in vivo, demonstrating ablation of MUC1+ pancreatic cancer xenograft in SCID mice. Figure 7A: SCID mice were inoculated subcutaneously with Human Colo357 pancreatic cancer cells on Day 0 and divided into three groups: Group [1] received 5 pg of chDMB5F3: ZZ-PE38 immunotoxin, Group [2] received 5 pg of non-specific matched isotype human Ig: ZZ-PE38 conjugate, and Group [3] Hepes buffer. Ail xenotransplanted mice of the three groups were injected on Days 1,4, 8, 11, 16, 24, 31 and 38. Tumor volumes were compared up to 49 days following cell inoculation. The histograms represent the average tumor volumes for each group, while each asterisk represents the value for an individual mouse. The y-axis to the left represents tumor volume (in mm³) for Groups 1 and 2; the y axis to the right represents tumor volumes in Group 3 (the Hepes buffer group) extended to 500 mm³ to include the larger tumor volumes in the Hepes control group. Two points in Group 3 with values above the 500 mm³ point represent tumor volumes of 705 mm³ and 1008 mm³. Figures 7B-7D show représentative mice from each group together with their tumor volumes at the conclusion ofthe 49-day tumor measurement period. Figure 7B: mice which received chDMB5F3: ZZ-PE38 immunotoxin, Figure 7C: mice which received non-specîfic human lg:ZZ-PE38 conjugate, and Figure 7D: mice which received only Hepes buffer.

DETAILED DESCRIPTION OF EMBODIMENTS

The present invention provides sequences of monoclonal antibodies (mAbs) directed against the MUC1 SEA α-β junction (termed the SEA domain), having a robust anti-cancer activity in vivo.

The invention provides sequences of antibodies directed against the MUC1 SEA domain, termed herein DMB5F3, DMB7F3, DMB4B4, DMB10F10, DMB4F4, DMB10B7, DMB13D11 and DMC209.

As shown in the Examples below, immunohistochemical staining with the mAb designated DMB5F3 of cells from a sériés of malignancies including lung, prostate, breast, colon, and pancreatic carcinomas revealed quantitative and qualitative différences between MUC1 expression on normal versus malîgnant cells: DMB5F3 strongly stained malignant cells in a near-circumferential pattern, whereas MUC1 in normal pancreatic and breast tissue showed only a weak apical pattern of positivity in ductal/acinar cells. Chimeric (mousehuman) DMB5F3 linked to ZZ-PE38 (ZZ being an IgG-binding protein fused to Pseudomonas exotoxin PE38) induced vigorous cytotoxicity of MUC1 + malignant cells in vitro. The intensity of cell killing by the anti-MUC1 DMB5F3:ZZ-PE38 protein-exotoxin construct correlated with the level of MUC1 expressed by the target cell, suggesting threshold of MUC1 expression is required for cell killing. In orderto demonstrate in vivo the effect of antibody DMB5F3 on tumor kill, MUC1+ Colo357 human pancreatic cancer cells were xenotransplanted into nude and SCID mice and then were treated with the chDMB5F3: ZZ-PE38 immunocomplex. In both transplant models (nude and SCID mice), chDMB5F3: ZZ-PE38 démonstrated significant in vivo anti-tumor activity, suppressing up to 90% of tumor volume in the SCID mice compared with concomitant Controls.

The present invention therefore provides antibodies for use in the treatment of MUC1expressing malignancies.

Therefore, in a first of its aspects, the present invention provides an isolated monoclonal antibody or antigen-binding fragment thereof which binds to the MUC1 SEA domain.

The term MUC1 SEA domain'' (also referred to herein as the MUC1 SEA module) refers to a highly conserved domain of 120 amino acids formed by the interaction of the MUC1 α-subunit with the extracellular portion of the MUC1 β-subunit. It is therefore positioned at the MUC1 α-β junction and is a stable cell membrane-fixed moiety; at no time is it shed from the cell surface (see Figures 1A, 1B and 1C, régions indicated by the ovals). The SEA domain as known in the art is defined as the région located between amino acids 264 and 384 of the human MUC1 protein (UniProtKB A0A087X2A4 (A0A087X2A4_HUMAN).

The MUC1 transmembrane glycoprotein (MUC-TM) is a heterodrmer consisting of an extracellular domain containing between 20 and 125 repeats of a 20 amino acid long sequence (termed the variable number tandem repeat, VNTR), a transmembrane domain, and a short cytoplasmic tail mediating intracellular signaling, MUC1 is auto-proteolytically cleaved within the SEA module. This results in a large extracellular a subunit containing the tandem repeat array bound in a strong non-covalent interaction to a transmembrane β subunît containing the transmembrane and cytoplasmic domains ofthe molécule, Specifically, the présent invention provides an isolated monoclonal antibody or antigenbinding fragment thereof which binds to MUC1 SEA domain, wherein said antibody comprises:

a. a heavy Chain complementarity determining région (CDRH) 1 denoted by SEQ ID NO. 25, CDRH2 denoted by SEQ ID NO. 26, CDRH3 denoted by SEQ ID NO. 27, and a light Chain complementarity determining région (CDRL) 1 denoted by SEQ ID NO, 28, a CDRL2 denoted by SEQ ID NO, 29, and a CDRL3 denoted by SEQ ID NO. 30; or b, a heavy chain complementarity determining région (CDRH) 1 denoted by SEQ ID NO. 31, CDRH2 denoted by SEQ ID NO. 32, CDRH3 denoted by SEQ ID NO, 33, and the light chain complementarity determining région (CDRL) 1 denoted by SEQ ID NO. 34, a CDRL2 denoted by SEQ ID NO. 35, and a CDRL3 denoted by SEQ ID NO. 36; or

f. a heavy chain complementarity determining région (CDRH) 1 denoted by SEQ ID

NO. 55, CDRH2 denoted by SEQ ID NO. 56, CDRH3 denoted by SEQ ID NO. 57, and the light Chain complementarity determining région (CDRL) 1 denoted by SEQ ID NO. 58, a CDRL2 denoted by SEQ ID NO. 59, and a CDRL3 denoted by SEQ ID NO. 60.

As indicated above, the présent invention provides isolated monoclonal antibodies that bind to MUC1 SEA domain. The term “antibody’ refers to a polypeptide encoded by an immunoglobulin gene that specifically binds and recognizes an antigen, in the présent case the MUC1 SEA domain.

The term “monoclonal antibody . “monoclonal antibodies” or mAb as herein defined refers to a population of homogenous antibodies, i.e., the individual antibodies comprising the population are identical except for possibly naturally occurring rare mutations. Monoclonal antibodies are directed against a single antigenic site (epitope).

Monoclonal antibodies may be prepared and purified by any method known in the art. For example, monoclonal antibodies may be prepared from B cells taken from the spleen or lymph nodes of immunized animais (e.g., rabbits, rats, mice, or monkeys).

Purification of monoclonal antibodies may be performed using any method known in the art, for example by affinity chromatography, namely, by using an affinity column to which a spécifie epitope (or antigen) is conjugated. Alternatively, purification of antibodies may be based on using protein A and protein G column chromatography.

An exemplary antibody structurai unit comprises a tetramer, as known in the art. Each tetramer is composed of two identical pairs of polypeptide chains, each pair having one light chain” and one “heavy chain. The N-terminus of each chain defines a variable région of about 100 to 110 or more amino acids primarily responsible for antigen (epitope) récognition.

Thus, the terms “heavy chain variable région (Vh) and “light chain variable région” (VJ refer to these heavy and light chains, respectively. More specifically, the variable région is subdivided into hypervariable and framework (FR) régions. Hypervariable régions hâve a high ratio of different amino acids in a spécifie position, relative to the most common amino acid in that position. Four FR régions which hâve more stable amino acids sequences separate the hypervariable régions. The hypervariable régions directly contact a portion of the antigen's surface. For this reason, hypervariable régions are herein referred to as complementarity determining réglons, or CDRs. the CDRs are positioned both at the heavy chain of the antibody (a heavy chain complementarity determining région) and at the light chain of the antibody (a light chain complementarity determining région) From N-terminal to C-terminal, both light and heavy chains comprise the domains FR1, CDR1, FR2, CDR2, FR3, CDR3 and FR4. The CDRs are primarily responsible for binding to an epitope of an antigen. The CDRs of each chain are typically referred to as CDR1,

CDR2, and CDR3, numbered sequentially starting from the N-terminus, and are also typically identified by the chain in which the CDR is located.

Thus, the complementarity determining régions CDRH1, CDRH2 and CDRH3 refer to the three complementarity determining régions starting from the N-terminus of the antibody's heavy chain (also referred to herein as heavy chain complementarity determining région) and the complementarity determining régions CDRL1, CDRL2 and CDRL3 refer to the three complementarity determining régions starting from the N-terminus of the antibody’s light chain (also referred to herein as light chain complementarity determining région).

The présent invention encompasses antigen-binding fragments of the isolated anti MUC1 SEA domain monoclonal antibody of the invention.

As used herein the term antigen binding fragment relates to a fragment of the full length antibody which retains the antibody's specificity of binding to MUC1 SEA domain. An antigen binding fragment encompasses but is not limited to Fv, single chain Fv (scFv), single chain Fv-Fc (scFv-Fc), Fab’, Fab, F(ab’)₂ and F(ab)₂.

Such fragments may be produced by any method known in the art, for example by proteolytic cieavage, using enzymes such as papain (to produce Fab fragments) or pepsin (to produce F(ab')₂ fragments).

Thus, in some embodiments the antibody according to the invention is wherein said antibody is an antibody fragment selected from the group consisting of a single-chaîn FvFc (scFv-Fc) molécule, single chain Fv (scFv), Fv, Fab’, Fab, F(ab’)₂, and F(ab)₂.

In spécifie embodiments the isolated monoclonal antibody of the invention or the antigenbinding fragment thereof binds to the MUC1 SEA domain.

As demonstrated in Example 5 below, administration of an immunocomplex comprising the antibody DMB5F3 and a cytotoxic moiety significantly reduced tumor volume in an in vivo cancer mode! in mice. Therefore, in spécifie embodiments the isolated monoclonal antibodies of the invention or the antigen-binding fragment thereof are effective in reducing tumor volume in a subject.

By the term “reducing” tumor volume in the context of the présent invention it is meant that the isolated monoclonal antibody of the invention or the antigen-binding fragment thereof lowers the size of the tumor as measured by any means known in the art, by at least about 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%, 11%, 12%, 13%, 14%, 15%, 16%, 17%, 18%, 19%, 20%, 21%, 22%, 23%, 24%, 25%, 26%, 27%, 28%, 29%, 30%, 31%, 32%,

33%, 34%, 35%, 36%, 37%, 38%, 39%, 40%, 41%, 42%, 43%, 44%, 45%, 46%, 47%,

48%, 49%, 50%, 51%, 52%, 53%, 54%, 55%, 56%, 57%, 58%, 59%, 60%, 61%, 62%,

63%, 64%, 65%, 66%, 67%, 68%, 69%, 70%, 71%, 72%, 73%, 74%, 75%, 76%, 77%,

78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%,

93%, 94%, 95%, 96%, 97%, 98%, 99% or about 100% as comparée! to the tumor size in the absence of the antibody of the invention or the antigen-binding fragment thereof. In certain embodiments the term reducing is meant to refer to a réduction of at least about 50%, 60%, 70%, 80%, or 90%.

In some embodiments the isolated anti-MUC1 SEA domain monoclonal antibody is a murine antibody, a chimeric antibody, a humanized antibody, or a human antibody.

The term chimeric antibody as used herein refers to an antibody that possesses antigen-binding variable domains from one species (e.g., the variable domains of a murine antibody) and constant domains of a different species (e.g., human).

The term humanized antibody as used herein refers to an antibody that is based on the structure of a non-human species (e.g., a mouse) whose amino acid sequence has been modified to increase its similarity to antibody variants produced naturally in humans.

The term human antibody as used herein refers to an antibody that possesses an amino acid sequence corresponding to that of an antibody produced by a human and/or has been made using any of the techniques for making human antibodies known in the art. This définition specifically excludes a humanized antibody that comprises non-human antigenbinding residues.

Methods for the préparation of chimeric, humanized, and human antibodies are well known in the art.

For preparing large quantities ofthe antibody (either chimeric, humanized, or human), a stable cell line expressing the antibody can be prepared, by transfecting cells (e.g., CHO cells) with the Ig expression vector containing both heavy and light chains ofthe antibody. The antibodies may then be manufactured for example in a bioreactor system. The antibodies may be purified to clinical grade using well established monoclonal antibody purification methods. Clones producing high levels of anti-MUC1 SEA domain antibody may then be selected and expanded based on antibody levels in the supernatant, as tested by any method known in the art, for example, a MUC1 SEA domain-specific ELISA assay. A master cell bank developed for the spécifie clone may serve as the starting growing material for ail clinical grade batches.

In some embodiments, the present invention provides an anti-MUC1 SEA domain isolated monoclonal antibody or antigen-binding fragment thereof, wherein said antibody or antigenbinding fragment thereof comprises a heavy Chain variable région and a light chain variable région, wherein said heavy chain variable région is encoded by a nucleic acid sequence which is at least 70%, or 75%, or 80%, or 85%, or 90% or more îdentical to the nucleic acid sequence denoted by SEQ ID NO. 1 and wherein said light chain variable région is encoded by a nucleic acid sequence which is at least 70%, or 75%, or 80%, or 85%, or 90% or more identical to the nucleic acid sequence denoted by SEQ ID NO. 2.

In other embodiments, the présent invention provides an anti-MUC1 SEA domain isolated monoclonal antibody or antigen-binding fragment thereof, wherein said antibody or antigenbinding fragment thereof is comprised of a heavy chain variable région and a light chain variable région, wherein said heavy Chain variable région is encoded by a nucleic acid sequence which is at least 70%, or 75%, or 80%, or 85%, or 90% or more identical to the nucleic acid sequence denoted by SEQ ID NO. 3 and wherein said light chain variable région is encoded by a nucleic acid sequence which is at least 70%, or 75%, or 80%, or 85%, or 90% or more identical to the nucleic acid sequence denoted by SEQ ID NO. 4.

In some further embodiments, the présent invention provides an anti-MUC1 SEA domain isolated monoclona! antibody or antigen-binding fragment thereof, wherein said antibody or antigen-binding fragment thereof is comprised of a heavy chain variable région and a light chain variable région, wherein said heavy chain variable région is encoded by a nucleic acid sequence which is at least 70%, or 75%, or 80%, or 85%, or 90% or more identical to the nucleic acid sequence denoted by SEQ ID NO. 5 and wherein said light chain variable région is encoded by a nucleic acid sequence which is at least 70%, or 75%, or 80%, or 85%, or 90% or more identical to the nucleic acid sequence denoted by SEQ JD NO. 6.

In some embodiments, the présent invention provides an anti MUC1-SEA domain isolated monoclonai antibody or antigen-binding fragment thereof, wherein said antibody or antigenbinding fragment thereof is comprised of a heavy chain variable région and a light chain variable région, wherein said heavy chain variable région is encoded by a nucleic acid sequence which is at least 70%, or 75%, or 80%, or 85%, or 90% or more identical to the nucleic acid sequence denoted by SEQ ID NO. 7 and wherein said light chain variable région is encoded by a nucleic acid sequence which is at least 70%, or 75%, or 80%, or 85%, or 90% or more identical to the nucleic acid sequence denoted by SEQ ID NO. 8.

In some embodiments, the présent invention provides an anti-MUC1 SEA domain isolated monoclonal antibody or antigen-binding fragment thereof, wherein said antibody or antigenbinding fragment thereof comprises a heavy chain variable région and a light chain variable région, wherein said heavy Chain variable région is encoded by a nucleic acid sequence which is at least 70%, or 75%, or 80%, or 85%, or 90% or more identical to the nucleic acid sequence denoted by SEQ ID NO. 9 and wherein said light chain variable région is encoded by a nucleic acid sequence which is at least 70%, or 75%, or 80%, or 85%, or 90% or more identical to the nucleic acid sequence denoted by SEQ ID NO. 10.

In some embodiments, the present invention provides an anti-MUC1 SEA domain isolated monoclonal antibody or antigen-binding fragment thereof, wherein said antibody or antigenbinding fragment thereof comprises a heavy chain variable région and a light chain variable région, wherein said heavy chain variable région is encoded by a nucleic acid sequence which is at least 70%, or 75%, or 80%, or 85%, or 90% or more identical to the nucleic acid sequence denoted by SEQ ID NO. 11 and wherein said light chain variable région is encoded by a nucleic acid sequence which is at least 70%, or 75%, or 80%, or 85%, or 90% or more identical to the nucleic acid sequence denoted by SEQ ID NO. 12.

In some embodiments, the present invention provides an anti-MUC1 SEA domain isolated monoclonal antibody or antigen-binding fragment thereof, wherein said antîbody or antigenbinding fragment thereof comprises a heavy chain variable région comprising the amino acid sequence denoted by SEQ ID NO. 13 or a variant thereof and a light chain variable région comprising the amino acid sequence denoted by SEQ ID NO. 14, or a variant thereof.

In other embodiments, the present invention provides an anti-MUC1 SEA domain isolated monoclonal antibody or antigen-binding fragment thereof, wherein said antibody or antigenbinding fragment thereof comprises a heavy Chain variable région comprising the amino acid sequence denoted by SEQ ID NO. 15 or a variant thereof and a light chain variable région comprising the amino acid sequence denoted by SEQ ID NO. 16, or a variant thereof.

In some further embodiments, the present invention provides an anti-MUC1 SEA domain isolated monoclonal antibody or antigen-binding fragment thereof, wherein said antibody or antigen-binding fragment thereof comprises a heavy chain variable région comprising the amino acid sequence denoted by SEQ ID NO. 17 or a variant thereof and a light chain variable région comprising the amino acid sequence denoted by SEQ ID NO. 18, or a variant thereof.

In some embodiments, the present invention provides an anti-MUC1 SEA domain isolated monoclonal antibody or antigen-binding fragment thereof, wherein said antibody or antigenbinding fragment thereof comprises a heavy chain variable région comprising the amino acid sequence denoted by SEQ ID NO. 19 or a variant thereof and a light chain variable région comprising the amino acid sequence denoted by SEQ ID NO. 20, or a variant thereof.

In certain embodiments, the present invention provides an anti-MUC1 SEA domain isolated monoclonal antibody or antigen-binding fragment thereof, wherein said antibody or antigenbinding fragment thereof comprises a heavy chain variable région comprising the amino acid sequence denoted by SEQ ID NO. 21 or a variant thereof and a light chain variable région comprising the amino acid sequence denoted by SEQ ID NO. 22, or a variant thereof.

In some embodiments, the present invention provides an anti-MUC1 SEA domain isolated monoclonai antibody or antigen-binding fragment thereof, wherein said antibody or antigenbinding fragment thereof comprises a heavy chain variable région comprising the amino acid sequence denoted by SEQ ID NO. 23 or a variant thereof and a light chain variable région comprising the amino acid sequence denoted by SEQ ID NO. 24, or a variant thereof.

In other embodiments the isoiated antibody according to the invention is wherein said antibody is an anti MUC1 SEA domain isoiated monoclonal antibody or antigen-binding fragment thereof, wherein said antibody comprises six CDR sequences as denoted by SEQ ID Nos. 25-30, and a heavy chain variable région comprising an amino acid sequence having at least 90% sequence identity to SEQ ID NO. 13 and a light chain variable région comprising an amino acid sequence having at least 90% sequence identity to SEQ ID NO: 14.

In other embodiments the isoiated antibody according to the invention is wherein said antibody is an anti MUC1 SEA domain isoiated monoclonal antibody or antigen-binding fragment thereof, wherein said antibody comprises six CDR sequences as denoted by SEQ ID Nos. 31-36, and a heavy chain variable région comprising an amino acid sequence having at least 90% sequence identity to SEQ ID NO: 15 and a light chain variable région comprising an amino acid sequence having at least 90% sequence identity to SEQ ID NO: 16.

In further embodiments the isoiated antibody according to the invention is wherein said antibody is an anti MUC1 SEA domain isoiated monoclonal antibody or antigen-binding fragment thereof, wherein said antibody comprises six CDR sequences as denoted by SEQ ID Nos. 37-42, and a heavy chain variable région comprising an amino acid sequence having at least 90% sequence identity to SEQ ID NO. 17 and a light chain variable région comprising an amino acid sequence having at least 90% sequence identity to SEQ ID NO. 18.

In various embodiments the isoiated antibody according to the invention is wherein said antibody is an anti MUC1 SEA domain isoiated monoclonal antibody or antigen-binding fragment thereof, wherein said antibody comprises six CDR sequences as denoted by SEQ ID Nos. 43-48, and a heavy chain variable région comprising an amino acid sequence having at least 90% sequence identity to SEQ ID NO. 19 and a light chain variable région comprising an amino acid sequence having at least 90% sequence identity to SEQ ID NO. 20.

In other embodiments the isoiated antibody according to the invention is wherein said antibody is an anti MUC1 SEA domain isoiated monoclonal antibody or antigen-binding fragment thereof, wherein said antibody comprises six CDR sequences as denoted by SEQ

ID Nos. 49-54, and a heavy chain variable région comprising an amino acid sequence having at least 90% sequence identîty to SEQ ID NO. 21 and a light Chain variable région comprising an amino acid sequence having at least 90% sequence identîty to SEQ ID NO. 22.

In further embodiments the isolated antibody according to the invention is wherein said antibody is an anti MUC1 SEA domain isolated monoclonal antibody or antigen-binding fragment thereof, wherein said antibody comprises six CDR sequences as denoted by SEQ ID Nos. 55-60, and a heavy chaîn variable région comprising an amino acid sequence having at least 90% sequence identify to SEQ ID NO. 23 and a light chain variable région comprising an amino acid sequence having at least 90% sequence identify to SEQ ID NO: 24.

In another embodiment, the present invention provides an isolated monoclonal antibody that competes with an antibody comprising:

(a) a heavy chain CDR1 comprising SEQ ID NO. 25, a heavy chain CDR2 comprising SEQ ID NO, 26, and a heavy chain CDR3 comprising SEQ ID NO. 27; and a light chain CDR1 comprising SEQ ID NO. 28, a light chain CDR2 comprising SEQ ID NO. 29, and a light chain CDR3 comprising SEQ ID NO. 30; or (b) a heavy chain CDR1 comprising SEQ ID NO. 31, a heavy chain CDR2 comprising SEQ ID NO. 32, and a heavy chain CDR3 comprising SEQ ID NO. 33; and a light Chain CDR1 comprising SEQ ID NO. 34, a light chain CDR2 comprising SEQ ID NO. 35, and a light chain CDR3 comprising SEQ ID NO. 36; or (c) a heavy chain CDR1 comprising SEQ ID NO. 37, a heavy chain CDR2 comprising SEQ ID NO. 38, and a heavy chain CDR3 comprising SEQ !D NO. 39; and a light chain CDR1 comprising SEQ ID NO. 40, a light chain CDR2 comprising SEQ ID NO. 41, and a light chain CDR3 comprising SEQ ID NO. 42; or (d) a heavy chain CDR1 comprising SEQ ID NO. 43, a heavy chain CDR2 comprising SEQ ID NO. 44, and a heavy chain CDR3 comprising SEQ ID NO. 45; and a light chain CDR1 comprising SEQ ID NO. 46, a light chain CDR2 comprising SEQ ID NO. 47, and a light chain CDR3 comprising SEQ ID NO. 48, or (e) a heavy chain CDR1 comprising SEQ ID NO. 49, a heavy chain CDR2 comprising SEQ ID NO. 50, and a heavy chain CDR3 comprising SEQ ID NO. 51; and a light chain CDR1 comprising SEQ ID NO. 52, a light chain CDR2 comprising SEQ ID NO. 53, and a light chain CDR3 comprising SEQ ID NO. 54; or (f) a heavy chain CDR1 comprising SEQ ID NO. 55, a heavy chain CDR2 comprising SEQ ID NO. 56, and a heavy chain CDR3 comprising SEQ ID NO. 57; and a light chain CDR1 comprising SEQ ID NO. 58, a light chain CDR2 comprising SEQ ID NO.

59, and a light chain CDR3 comprising SEQ ID NO. 60.

The nucleic acid sequences encoding the heavy and light chains ofthe antibodies termed herein DMB5F3, DMB7F3, DMB4B4, DMB4F4, DMB10B7, and DMC209 are detailed in Table 1 below. in addition, the amino acid sequences ofthe heavy and light chains ofthe antibodies described herein are detailed in Table 2 below. Furthermore, the sequences of the CDRs of the above antibodies are displayed in Table 3 below.

DMB4B4 and DMB10F10 hâve identical amino acid sequences.

DMB4F4 is mlg-gammal. DMB10B7 and DMB13D11 are mlgA. However, the variable régions of ail three antibodies: DMB4F4, DMB10B7 and DMB13D11 are identical.

Table 1: Nucleic acid sequences

SEQ ID NO.	Sequence	Description
1	ATGAGAGTGCTGATTCTTTTGTGCCTGTTCACAGCCTTTCCTGGTG TCCTGTCTGATGTGCAGGTTCAGGAGTCAGGACCTGACCTGGTGAA ACCTTCTCAGTCACTTTCTCTCACCTGCACTGTCACTGGCCACTCC ATCACCAGAGGTTCTAGCTGGCACTGGATCCGGCAGTTTCCAGGAA ACAAACTGGAGTGGATGGGATACATACATTACGGTGGTGGCACTTC CTACAACCCATCTCTCAAAAGTCGAATCTCTATCACTCGAGACACA TCCAAGAACCAGTTCTTCCTGCAGTTGAATTCTGTGACTACTGAGG ACACAGCCACATTTTTCTGTGCACGGTATTCCTACGATATTACCTA CCGCTGGTTCTTCGATGTCTGGGGCGCAGGGACCACGGTCATCGTC TCCTCA	DMB5F3 Heavy chain nucleic acid sequence
2	ATGGTATCCACACCTCAGTTCCTTGTATTTTTGCTTTTCTGGATTC CAGCCTCCAGAGGTGACATCTTGCTGACTCAGTCTCCAGCCATCCT GTCTGTGAGTCCAGGAGAAAGAGTCAGTTTCTCCTGCAGGGCCAGT CAGAACATTGGCACAAGCATACACTGGTATCAGCAAAGAAAAAATG GTTCTCCAAGACTTCTCATAAAGTATGCTTCTGAGTCTATCTCTGG GATTCCTTCCAGGTTTAGTGGCAGCGGATCTGGGACAGATTTTACT CTTTCCATCAACAGTGTGGAGTCTGAAGATATGGCAGATTATTACT GTCAACAAAATAATAACTGGCCGCTCACGTTCGGTGCTGGGACCAA GCTGGAACTGAAA	DMB5F3 Light chain nucleic acid sequence
3	ATGGAATGGCCTTGTATCTTTCTCTTCCTCCTGTCAGTAACTGAAG GTGTCCACTCCCAGGTTCACCTTCAGCAGTCTGGGGCTGAGCTGGT GAGGCCTGGGTCCTCAGTGAAGATTTCCTGTAAGGCTTCTGGCTAT GAATTCAATAGTTTCTGGATGAACTGGGTGAAGCAGAGGCCTGGAC AGGGTCTTGAGTGGATTGGAQAGATTTATCCTGGAGATGGTGATAC TAACTACAATGGAAAATTCAAGGGTAAAGCCACACTGACTGCTGAC AAATCCTCCAGCACAGCCTACATGCAGCTCAGCAGCCTAACATCTG	DMB4B4 Heavy chain nucleic acid sequence

	AGGACTCTGGGATTTACTTCTGTGCAAGAGGATACÀAGGCCTGGTT TATTTACTGGGGCCAAGGGACTCTGGTCACTGTCTCTGAA
4	ATGGTATCCACACCTCAGTTCCTTGTATTTTTGCTTTTCTGGATTC CAGCCTCCAGAGGTGACGTCTTGCTGACTCAGTCTCCAGCCATTCT GTCTGTGAGTCCAGGAGAAAGAGTCAGTTTCTCCTGCAGGGCCAGT CAGAACATTGGCACAAGCATACACTGGTATCAGCAAAGCACAAATG GTTCTCCAAGGCTTATCATAAAGTATGCTTCTGAGTCTCTCTCTGG GATCCCTTCCAGGTTTAGTGGCAGTGGATCAGGGACAGATTTTACT CTTACCATCAACAGTGTGGAGTCTGAGGATATTGCAGATTATTACT GTCAACAAAGTAATGGCTGGCCGCTCACGTTCGGTGGTGGGACCAA GCTGGAGCTGAAA	DMB4B4 Light chain nucleic acid sequence
5	ATGGGATGGAGCTGGATCTTTCTCTTCCTCCTGTCAGGAACTGCAG GTGTCCTCTCTGAGGTCCAGCTGCAACAGTCTGGACCTGAACTGGT GAAGCCTAAAACTTCAATGAAGATATCCTGCAAGGCCTCTGGTTAC TCATTCACTGACTTCACCATGAACTGGGTGAAGCAGAGCCATGGAA AAAACCCTGAGTGGATTGGACTTATTACTCCTTACAATGGTGGAAC TAGTTACAACCAGAAGTTCAAGGGCAAGGCCACATTTACTGTAGAC AGGTCATCCAGCACTGCCTACATGGAACTCCTCAGTCTGACATCTG AGGACTCTGCGGTCTATTACTGTGCAAGAGGCCTGACCTATTTTGA CCAGTGGGGCCAAGGCACCACTCTCACAGTCTCCTCA	DMB4F4 Heavy chain nucleic acid sequence
6	ATGGATTTTCAAGTGCAGATTTTCAGCTTCCTGCTAATGAGTGCCT CAGTCATAATGTCCAGGGGACAAATTGTTCTCACCCAGTCTCCAGC ACTCATGTCTGCATCTCCAGGGGAGAAGGTCACCATGACCTGCAGT GCCAGCTCAAGTGTAAGTTACATGTACTGGTATCAGCAGAAGCCAA CATCCTCCCCCAAACCCTGGATTTTGCTCACTTCCAACCTGGCTTC TGGAGTCCCTACTCGCTTCAGTGGCAGTGGGTCTGGGACCTCTTAC TCTCTCACAATCAGCAGCATGGAGGCTGAAGATGCTGCCACTTATT ACTGCCAGCAGTGGAATAGTAAACCACCCATCACGï'TCGGAGGGGG GACCAAGCTGGAAATAAAA	DMB4F4 Light chain nucleic acid sequence
7	ATGGGATGGAGCTGGATCTTTCTCTTCCTCCTGTCAGGAACTGCAG GTGTCCTCTCTGAGGTCCAGCTGCAACAGTCTGGACCTGAACTGGT GAAGCCTAAAACTTCAATGAAGATATCCTGCAAGGCCTCTGGTTAC TCATTCACTGACTTCACCATGAACTGGGTGAAGCAGAGCCATGGAA AAAACCCTGAGTGGAT T GGACTTATTACTCCTTACAATGGTGGAAC TAGTTACAACCAQAAGTTCAAGGGCAAGGCCACATTTACTGTAGAC AGGTCATCCAGCACTGCCTACATGGAACTCCTCAGTCTGACATCTG AGGACTCTGCGGTCTATTACTGTGCAAGAGGCCTGACCTATTTTGA CCAGTGGGGCCAAGGCACCACTCTCACAGTCTCCTCA	DMB10B7 Heavy chain nucleic acid sequence
8	ATGGATTTTCAAGTGCAGATTTTCAGCTTCCTGCTAATGAGTGCCT CAGTCATAATGTCCAGGGGACAAATTGTTCTCACCCAGTCTCCAGC ACTCATGTCTGCATCTCCAGGGGAGAAGGTCACCATGACCTGCAGT GCQAGCTCAAGTGTAAGTTACATGTACTGGTATCAGCAGAAGCCAA CATCCTCCCCCAAACCCTGGATTTTGCTCACTTCCAACCTGGCTTC TGGAGTCCCTACTCGCTTCAGTGGCAGTGGGTCTGGGACCTCTTAC TCTCTCACAATCAGCAGCATGGAGGCTGAAGATGCTGCCACTTATT ACTGCCAGCAGTGGAATAGTAAACCACCCATCACGTTCGGAGGGGG GACCAAGCTGGAAATAAAA	DMB10B7 Light chain nucleic acid sequence
9	ATGGCTGTCCTGGGGCTGCTTCTCTGCCTGGTGACGTTCCCAAGCT GTGTCCTGTCCCAGGTGCAGCTGAAGGAATCAGGACCTGGCCTAGT GGCGCCCTCACAGAACCTGTCCATCACATGCACTGTCTCAGGTTTC TCATTAACCGACTATGGTGTAAACTGGGTTCGCCAGCCTTCAGGAA	DMB7F3 Heavy chain nucleic acid sequence

	AGGGTCTGGAGTGGCTGGGAGAAATATGGGCTGGTGGAACTACATT CTATAATTCAGCTCTCAAATCCAGACTGACCATCACCAAGGACAAC TCCAAGAGCCAAGTTTTCTTAGAAATGAACAGTCTGCAAAGTCATG ACACAGCCATGTACTATTGTGCCAAACGGCTTAACTGGGACAGTTC TATGGACTACTGGGGTCAAGGAACCTCAGTCACCGTCTCCTCA
10	ATGGAGACAGACACACTCCTGTTATGGGTACTGCTGCTCTGGGTTC CAGGTTCCACTGGTGACATTGTGCTGACACAGTCTCCTGCTTCCTT TGCTGTATCTCTGGGACAGAGGGCCACCATCTCATGCAGGGCCAGC GAAAGTGTCAGTACGTCTGCCTATAATTTTCTGCACTGGTACCAGC AGAAACCTGGACAGCCACCCAAACTCCTCATCTATCTTGCATCCAA CCTAGAATCTGGGGTCCCTGCCAGGTTCAGTGGCAGTGGGTCTGGG ACAGACTTCACCCTCAACATCCATCCTGTGGAGGAGGAGGATGCTG CAACCTATTACTGTCAGCACAGTAGGGAGCTTCCGTACACGTTCGG AGGGGGGACCAAGTTGGAAATAAAA	DMB7F3 Light chain nucleic acid sequence
11	ATGAGAGTGCTGATTCTTTTGTGGCTGTTCACAGCCTTTCCTGGTA TCCTGTCTGATGTGCAGCTTCAGGAGTCGGGACCTGGCCTGGTGAA ACCTTCTCAGTCTCTGTCCCTCACCTGCACTGTCACTGGCTACTCA ATCACCAGTGATTATGCCTGGAACTGGATCCGGCACTTTCCAGGAA ACAAAC T GGAGTGGAT GGGCTACATAAGCTACAGTGGTAGCACTAG CTACAACCCATCTCTCAAAAGTCGAATCTCTATCACTCGAGACACA TCCAAGAACCAGTTCTTCCTGCAGTTGAATTCTGTGACTACTGAGG ACACAGCCACATATTACTGTGCAAGAQATGGTTACTACACCTTTGC TTTCTGGGGCCAAGGGACTCTGGTCACTGTCTCTGCA	DMC209 Heavy chain nucleic acid sequence
12	ATGAAGTTGCCTGTTAGGCTGTTGGTGCTGATGTTCTGGATTCCTG CTTCCAGCAGTGATGTTGTGATGACCCAAACTCCACTCTCCCTGCC TGTCAGTCTTGGAGATCAAGCCTCCATCTCTTGCAGATCTAGTCAG AGCCTTGTACACAGTAATGGAAACACCTATTTACATTGGTACCTGC AGAAGCCAGGCCAGTCTCCAAAGCTCCTGATCTACAAAGTTTCCAA CCGATTTTCTGGGGTCCCAGACAGGTTCAGTGGCAGTGGATCAGGG ACAGATTTCACACTCAAGATCAGCAGAGTGGAGGCTGAGGATCTGG GAGTTTATTTCTGCTCTCAAAGTACACATGTTCCGTACACGTTCGG AGGGGGGACCAAGCTGGAAATAAAA	DMC209 Light chain nucleic acid sequence

Table 2: Amino acid sequences

SEQ ID NO.	Sequence	Description
13	MRVLILLCLFTAFPGVLSDVQVQESGPDLVKPSQSLSLTCTVTGHS ΙΤΚββδΚΗΜΙΚΟΓΡαΝΚΑΕΚΜΰΥΙΗΥΟΟΟΤδΥΝΡδΙιΚΒΡΙΞΙΤΚΟΤ SKNQFFLQLNSVTTEDTATFFCARYSYDITYRWFFDVWGAGTTVIV SS	DMB5F3 Heavy chain amino acid sequence
14	MVSTPQFLVFLLFWIPASRGDILLTQSPAILSVSPGERVSFSCRAS QNIGTSIHWYQQRKNGSPRLLIKYASESISGIPSRFSGSGSGTDFT	DMB5F3 Light chain amino acid

	LSINSVESEDMADYYCQQNNNWPLTFGAGTKLELK	sequence
15	MEWPCIFLFLLSVTEGVHSQVHLQQSGAELVRPGSSVKISCKASGY EFNSFWMNWVKQRPGQGLEWIGQIYPGDGDTNYNGKFKGKATLTAD KSSSTAYMQLSSLTSEDSGIYFCARGYKAWFIYWGQGTLVTVSE	DMB4B4 Heavy chain amino acid sequence
16	MVSTPQFLVFLLFWIPASRGDVLLTQSPAILSVSPGERVSFSCRAS QNIGTSIHWYQQSTNGSPRLIIKYASESLSGIPSRFSGSGSGTDFT LTINSVESEDIADYYCQQSNGWPLTFGGGTKLELK	DMB4B4 Light chain amino acid sequence
17	MGWSWIFLFLLSGTAGVLSEVQLQQSGPELVKPKTSMKISCKASGY SFTDFTMNWVKQSHGKNPEWIGLITPYNGGTSYNQKFKGKATFTVD RSSSTAYMELLSLTSEDSAVYYCARGLTYFDQWGQGTTLTVSS	DMB4F4 Heavy chain amino acid sequence
18	MDFQVQIFSFLLMSASVIMSRGQIVLTQSPALMSASPGEKVTMTCS ASSSVSYMYWYQQKPTSSPKPWILLTSNLASGVPTRFSGSGSGTSY SLTISSMEAEDAATYYCQQWNSKPPITFGGGTKLEIK	DMB4F4 Light chain amino acid sequence
19	MGWSWIFLFLLSGTAGVLSEVQLQQSGPELVKPKTSMKISCKASGY SFTDFTMNWVKQSHGKNPEWIGLITPYNGGTSYNQKFKGKATFTVD RSSSTAYMELLSLTSEDSAVYYCARGLTYFDQWGQGTTLTVSS	DMB10B7 Heavy chain amino acid sequence
20	MDFQVQIFSFLLMSASVIMSRGQIVLTQSPALMSASPGEKVTMTCS ASSSVSYMYWYQQKPTSSPKPWILLTSNLASGVPTRFSGSGSGTSY SLTISSMEAEDAATYYCQQWNSKPPITFGGGTKLEIK	DMB10B7 Light chain amino acid sequence
21	MAVLGLLLCLVTFPSCVLSQVQLKESGPGLVAPSQNLSITCTVSGF SLTDYGVNWVRQPSGKGLEWLGEIWAGGTTFYNSALKSRLTITKDN SKSQV FLEMN S LQS H DTAMYYCAKRLNWDS SMDYWGQGTSVTVS S	DMB7F3 Heavy chain amino acid sequence
22	METDTLLLWVLLLWVPGSTGDIVLTQSPASFAVSLGQRATISCRAS ESVSTSAYNFLHWYQQKPGQPPKLLIYLASNLESGVPARF3GSGSG TDFTLNIHPVEEEDAATYYCQHSRELPYTFGGGTKLEIK	DMB7F3 Light chain amino acid sequence
23	MRVLILLWLFTAFPGILSDVQLQESGPGLVKPSQSLSLTCTVTGYS ITSDYAWNWIRHFPGNKLEWMGYISYSGSTSYNPSLKSRISITRDT SKNQFFLQLNSVTTEDTATYYCARDGYYTFAFWGQGTLVTVSA	DMC209 Heavy chain amino acid sequence
24	MKLPVRLLVLMFWIPASSSDVVMTQTPLSLPVSLGDQASISCRSSQ SLVHSNGNTYLHWYLQKPGQSPÎÎLLIYKVSNRFSGVPDRFSGSGSG TDFTLKISRVEAEDLGVYFCSQSTHVPYTFGGGTKLEIK	DMC209 Light chain amino acid sequence

The CDR sequences are highlighted within the heavy and the light chain amino acid sequences and are listed in Table 3.

Table 3; CDR amino acid sequences

SEQ ID NO.	Sequence	Description
25	RGSSWH	DMB5F3 Heavy chain CDR H1
26	YIHYGGGTSYNPSLKS	DMB5F3 Heavy chain CDR H2
27	YSYDITYRWFFDV	DMB5F3 Heavy chain CDR H3
28	RASQNIGTSIH	DMB5F3 Light chain CDR L1
29	YASESIS	DMB5F3 Light chain CDR L2
30	QQNNNWPLT	DMB5F3 Light chain CDR L3
31	SFWMN	DMB4B4 Heavy chain CDR H1
32	QIYPGDGDTNYNGKFKG	DMB4B4 Heavy chain CDR H2
33	GYKAWFIY	DMB4B4 Heavy chain CDR H3
34	RASQNIGTSIH	DMB4B4 Light chain CDR L1
35	YASESLS	DMB4B4 Light chain CDR L2
36	QQSNGWPLT	DMB4B4 Light chain CDR L3
37	DFTMN	DMB4F4 Heavy chain CDR H1
38	LITPYNGGTSYNQKEKG	DMB4F4 Heavy Chain CDR H2
39	GLTYFDQ	DMB4F4 Heavy chain CDR H3
40	SASSSVSYMY	DMB4F4 Light chain CDR L1
41	LTSNLAS	DMB4F4 Light chain CDR L2
42	QQWNSKPPIT	DMB4F4 Light chain CDR L3
43	DFTMN	DMB10B7 Heavy chain CDR H1
44	LITPYNGGTSYNQKFKG	DMB10B7 Heavy chain CDR H2
45	GLTYFDQ	DMB10B7 Heavy chain CDR H3
46	SASSSVSYMY	DMB10B7 Light chain CDR L1
47	LTSNLAS	DMB10B7 Light chain CDR L2
48	QQWNSKPPIT	DMB10B7 Light chain CDR L3
49	DYGVN	DMB7F3 Heavy chain CDR H1
50	EIWAGGTTFYNSALKS	DMB7F3 Heavy chain CDR H2
51	RLNWDSSMDY	DMB7F3 Heavy chain CDR H3
52	RASESVSTSAYNFLH	DMB7F3 Light chain CDR L1

53	LASNLES	DMB7F3 Light Chain CDR L2
54	QHSRELPYT	DMB7F3 Light Chain CDR L3
55	SDYAWN	DMC209 Heavy Chain CDR H1
56	YISYSGSTSYNPSLKS	DMC209 Heavy Chain CDR H2
57	DGYYTFAF	DMC209 Heavy Chain CDR H3
58	RSSQSLVHSNGNTYLH	DMC209 Light Chain CDR L1
59	KVSNRFS	DMC209 Light Chain CDR L2
60	SQSTHVPYT	DMC209 Light chain CDR L3

The genomic dérivation of the antibodies is shown in the following Tables.

Table 4: Group I, DMB5F3

Sequence	V-gene and allele	V-region identity % (nt)	J-gene and allele	D-gene and allele	AA Junction
V_H	Musmus IGHV3- 1*02 F	95.14% (274/288 nt)	Musmus IGHJ1*01 F	Musmus IGHD1-1*01 F	CARYSYDI TYRWFFDVW
V_l	Musmus IGKV548*0 1F	96.06% (268/279 nt)	Musmus IGKJ5*01 F	—	CQQNNN WPLTF

Table 5: Group II, DMB7F3

Sequence	V-gene and allele	V-region identity % (nt)	J-gene and allele	D-gene and allele	AA Junction
Vh	Musmus IGHV2-6- 5*0 1F	93.68% (267/285 nt)	Musmus IGHJ4*01 F	Musmus IGHD4-V01 F	CAKRLNW DSSMDYW

Vl

Musmus IGKV312*0 1F

96.56% (281/291 nt)

Musmus IGKJ2*01 F

—“

CQHSREL PYTF

Table 6: Group III, DMB4B4, DMB10F10

Sequence	V-gene and allele	V-région identity % (ni)	J-gene and ailele	D-gene and allele	AA Junction
Vu	Musmus IGHV1- 80*0 1F	92.01% (265/288 nt)	Musmus IGHJ3*01 F	Musmus IGHD1-2*01 F	CARGYKA WFIYW
Vl	Musmus IGKV548*0 1F	96.77% (270/279 nt)	Musmus IGKJ5*01 F	—	CQQSNS WPLTF

Table 7: Group IV, DMB4F4, DMB10B7, DMB13D11

Sequence	V-gene and allele	V-region identity % (nt)	J-gene and allele	D-gene and allele	AA Junction
V_H	Musmus IGHV1180 1F, or Musmus IGHV1- 260 1F	88.89% (256/288 nt)	Musmus IGHJ2*01 F	Musmus IGHD5-1*01 P	CARGLTY FDQW
Vl	Musmus IGKV468*0 1F	97.46% (269/276 nt)	Musmus IGKJ2*01 F	—	CQQWNSK PPITF

Table 8: DMC209

Sequence	V-gene and allele	V-region identity % (nt)	J-gene and allele	D-gene and allele	AA Junction
V_H	Musmus IGHV32*0 F	99.65% (287/288 nt)	Musmus IGHJ3*01 F	Musmus IGHJ3*01 F	CARDGY YTFAFW
V_L	Musmus IGKV1- 110*01 F	100.0% (294/294 nt)	Musmus IGKJ2*01 F		CSQSTH VPYTF

The present invention also encompasses variants of the heavy and light chain variable régions. The variants may include mutations in the complementarity determining régions of the heavy and light chains which do not alter the activity of the antibodies herein described, or in the framework région.

By the term “variant’ it is meant sequences of amino acids or nucléotides different from the sequences specifically identified herein, in which one or more amino acid residues or nucléotides are deleted, substituted, or added.

It should be apprecîated that by the term added , as used herein it is meant any addition of amino acid residues to the sequences described herein.

Variants encompass various amino acid substitutions. An amino acid substitution” is the resuit of replacing one amino acid with another amino acid which has similar or different structural and/or Chemical properties. Amino acid substitutions may be made based on similarity in polarity, charge, solubility, hydrophobicity, hydrophilicity, and/or the amphipathic nature of the residues involved.

Typically, variants encompass conservative amino acid substitutions. Conservative substitution tables providing functionally similar amino acids are well known in the art. For example, nonpolar (hydrophobie) amino acids include alanine, leucine, isoleucine, valine, proline, phenylaianine, tryptophan, and méthionine; polar neutrai amino acids include glycine, serine, threonine, cysteine, tyrosine, asparagine, and glutamine; positively charged (basic) amino acids include arginine, lysine, and histidine; and negatively charged (acidic) amino acids include aspartic acid and glutamic acid.

Each of the following eight groups contains other exemplary amino acids that are conservative substitutions for one another:

1) Alanine (A), Glycine (G);

2) Aspartic acid (D), Giutamic acid (E);

3) Asparagine (N), Glutamine (Q);

4) Arginine (R), Lysine (K);

5) Isoleucine (I), Leucine (L), Méthionine (M), Valine (V);

6) Phenylalanine (F), Tyrosine (Y), Tryptophan (W);

7) Serine (S), Threonine (T); and

8) Cysteine (C), Méthionine (M).

Conservative nucleic acid substitutions are nucleic acid substitutions resulting in conservative amino acid substitutions as defined above.

Variants in accordance with the invention also encompass non-polar to polar amino acid substitutions and vice-versa.

As used herein, the term amino acid” or “amino acid residue” refers to naturaliy occurring and synthetic amino acids, as well as amino acid analogs and amino acid mimetics that fonction similarly to the naturally occurring amino acids.

Variant sequences refer to amino acids or nucleic acids sequences that may be characterized by the percentage ofthe identity oftheir amino acid or nucléotide sequences with the amino acid or nucléotide sequences described herein (for example, the amino acid or nucléotide sequences ofthe heavy and light chains of the antibodies herein described). In some embodiments, variant sequences as herein defined refer to nucleic acid sequences that encode the heavy and light chain variable régions, each having a sequence of nucléotides with at least 70% or 75% of sequence identity, around 80% or 85% of sequence identity, around 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98% or 99% of sequence identity when compared to the sequences ofthe heavy and light chain variable régions described herein.

In some other embodiments, variant sequences as herein defined refer to amino acid sequences ofthe heavy and light chain variable régions, each having a sequence of amino acids with at least 70% or 75% of sequence identity, around 80% or 85% of sequence identity, around 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98% or 99% of sequence identity when compared to the sequences of the heavy and light chain variable régions described herein.

By the term ‘ activity of the antibodies it is meant the ability of the antibodies to bind MUC1 SEA domain, and preferably to médiate cell cytotoxicity either alone or as part of an immunocomplex with a cytotoxic moiety. The activity of the antibodies can be measured in vivo or in vitro using methods well known in the art, e.g., as described in the Examples below.

The binding of the antibody of the invention to its target protein may be measured for example using ELISA, biolayer interferometry (BLI), Western blot or immunofluorescence assays (IFA).

The biological activity of the antibodies can be measured for example in an in vivo cancer model, for example as detailed in the Examples below.

In another one of its aspects the présent invention provides an isolated nucleic acid molécule comprising a nucléotide sequence encoding an antibody or antigen-binding fragment thereof according to the invention.

The term “nucleic acid’ or “nucleic acid molécule as herein defined refers to a polymer of nucléotides, which may be either single- or double-stranded, which is a polynucleotide such as deoxyribonucleic acid (DNA), and, where appropriate, ribonucleic acid (RNA). The terms should also be understood to include, as équivalents, analogs of either RNA or DNA made from nucléotide analogs, and, as applicable to the embodiment being described, single-stranded (such as sense or antisense) and double-stranded polynucleotides. The term DNA used herein also encompasses cDNA, i.e., complementary or copy DNA produced from an RNA template by the action of reverse transcriptase (RNA-dependent DNA polymerase).

The invention further provides an expression vector comprising the isolated nucleic acid molécule as herein defined.

“Expression vector sometimes referred to as “expression vehicle” or “expression construct’, as used herein, encompasses vectors such as plasmids, viruses, bactériophage, integratable DNA fragments, and other vehicles, which enable the intégration of DNA fragments into the genome of the host. Expression vectors are typically self-replicating DNA or RNA constructs containing the desired gene or its fragments, and operably linked genetic control éléments that are recognized in a suitable host cell and effect expression of the desired genes. These control éléments are capable of effecting expression within a suitable host. The expression vector in accordance with the invention may be competent with expression in bacterîal, yeast, or mammalian host cells, to name but few.

In y et another one of its aspects the présent invention provides a host cell transfected with the isolated nucleic acid molécule according to the invention or with the expression vector according to the invention.

The term host cells” as used herein refers to cells which are susceptible to the introduction of the isolated nucleic acid molécule according to the invention or with the expression vector according to the invention. Preferably, said cells are mammalian cells, for example CHO cells or NSO cells. Transfection ofthe isolated nucleic acid molécule or the expression vector according to the invention to the host cell may be performed by any method known in the art.

In yet another one of its aspects the présent invention provides an immunoconjugate comprising the antibody or antigen-binding fragment thereof according to the invention and an additional cytotoxic or therapeutic agent as defined herein below.

The terms immunoconjugate”, immunocomplex are used interchangeably herein and refer to an antibody or antigen-binding fragment thereof according to the invention that is conjugated (linked or joined) to an additional agent. Immunoconjugates may be prepared by any method known to a person skilled in the art, for example, by cross-linking the additional agent to the antibody according to the invention or by recombinant DNA methods.

In certain embodiments, the immunoconjugate is an immunotoxin whereby the antibody or antigen-binding fragment thereof according to the invention is conjugated to a cytotoxic agent. The term cytotoxic agent as used herein refers to any agent that exerts a cytotoxic effect on a cell upon contact. Such cytotoxic agents are well known to a person skilled in the art. Examples of cytotoxic agents that may be used in the immunocomplex of the invention include, but are not limited to alkylating drugs, anthracyclines, pyrimidine dérivatives, vinca alkaloids, photodynamic drugs, piatinum-containing compounds, taxanes, topoisomerase inhibitors, ribosome inactivating agents (e.g., gelonin), agents that induce DNA damage (e.g., calicheamicin), tubulin inhibitors (e.g., emtansine), anti-mitotic agents (e.g., monomethyl auristatin), or bacterial toxins. The cytotoxic agents may also be radioisotopes or cytotoxic antibodies. In one embodiment, the toxic agent is Pseudomonas exotoxin, e.g., ZZ-PE38 (ZZ IgG-binding protein fused to Pseudomonas exotoxin).

The présent invention also encompasses bispecific antibodies capable of binding to two separate targets or epitopes, wherein said bispecific antibody comprises the antibody or antigen-binding fragment thereof according to the invention and an additional antibody or antigen-binding fragment thereof.

Therefore, in a spécifie embodiment, the présent invention provides an immunocomplex comprising an isolated monoclonal antibody or antigen-binding fragment thereof which binds to MUC1 SEA domain, wherein said antibody comprises:

a heavy chain complementarity determining région (CDRH) 1 denoted by SEQ ID NO. 25, CDRH2 denoted by SEQ ID NO. 26, CDRH3 denoted by SEQ ID NO. 27, and a light chain complementarity determining région (CDRL) 1 denoted by SEQ ID NO. 28, a CDRL2 denoted by SEQ ID NO. 29, and a CDRL3 denoted by SEQ ID NO. 30, and a cytotoxic agent being pseudomonas exotoxin, and wherein said immunocomplex reduces tumor volume upon administration to a subject with cancer.

The anti MUC1 SEA domain antibody of the invention may be administered in combination with at least one additional therapeutic agent.

The term additional therapeutic agent’ used herein refers to any agent that may be used for treating a disease or disorder, e.g., cancer.

In certain embodiments the additional therapeutic agent is an additional antibody. The term additional antibody” as herein defined refers to antibodies of the invention (namely to the combined use of at least two antibodies of the invention) as well as to an antibody, which is not the antibody according to the invention, which may be used in combination with the antibody of the invention for treating a disease or disorder, e.g., cancer. Such other antibodies include but are not limited to anti-CD22 antibodies, anti-CD30 antibodies, antiHER2 receptor antibodies, anti-VEGF antibodies, anti-EGFR antibodies, anti-tumor associated antigen (TAA) antibodies, and anti-check point inhibitors.

The additional therapeutic agent may also be a chemotherapeutîc agent, or an antiinflammatory agent.

The présent invention further provides a pharmaceutical composition comprising as an active ingrédient at least one isolated anti-MUCI SEA antibody of the invention, or antigenbinding fragment thereof or the immunoconjugate as herein defined and a pharmaceutically acceptable carrier, excipient, or diluent.

In a spécifie embodiment, said pharmaceutical composition is for use in the treatment of a disease or diorder associated with over-expression of MUC1.

The term “a disease or diorder associated with over-expression of MUC1” is used herein at its broadest sense and refers to any disease which is characterized by aberrant expression of MUC1. In a spécifie embodiment, the disease or disorder associated with over-expression of MUC1 is cancer. Examples include, but are not limited to, lung carcinoma, prostate carcinoma, breast carcinoma, ovarian carcinoma, colon carcinoma, small intestinal carcinoma, pancreatic carcinoma, gastric carcinoma, liver cancer, multiple myeloma, or acute myelogenous leukemia.

In other embodiments, the disease or disorder associated with over-expression of MUC1 is an autoimmune or inflammatory disease. Non-limiting examples of an autoimmune or inflammatory disease include rheumatoid arthritis, psoriatic arthntis, systemic lupus erythematosus, amyloidosis, and autoimmune pancreatitis.

In other embodiments, said disease or disorder is a non-ma lignant abnormal growth condition, e.g., cysts, for example clinicaîly significant rénal cysts, large, non-functional thyroid cysts and thyroid masses, hepatic cysts and the like.

The “pharmaceutical composition of the invention generally comprises the antibody or any antigen-binding fragment thereof as herein defined and a buffering agent, an agent which adjusts the osmolarity of the composition and optionally, one or more pharmaceutically acceptable carriers, excipients and/or diluents as known in the art.

As used herein the term “pharmaceutically acceptable carrier, excipient or diluent includes any solvent, dispersion medium, coating, antibacterial and antifungai agent, and the like, as known in the art. The carrier can be a solvent or dispersion medium containing, for example, water, éthanol, polyol (for example, glycerol, propylene glycol, and liquid polyethylene glycol, and the like), suitable mixturesthereof, and vegetable oils. Each carrier should be both pharmaceutically and physiologically acceptable in the sense of being compatible with the other ingrédients and not injurious to the subject. Except as any conventional media or agent is incompatible with the active ingrédient, iis use in the therapeutic composition is contemplated.

In other embodiments the pharmaceutical composition accordîng to the invention further comprises an additional therapeutic agent. Non-iimiting examples of additional therapeutic agents include anti MUC1 antibodies, anti-CD22 antibodies, anti-CD30 antibodies, antiHER2 receptor antibodies, anti-VEGF antibodies, anti-EGFR antibodies, anti-TAA antibodies and checkpoint inhibitors.

The présent invention also provides methods of treatment or amelioration of a disease or disorder associated with over-expression of MUC1 (e.g. cancer) comprising administering to a subject in need thereof a therapeutically effective amount of the isolated monoclonal antibody or antigen-binding fragment thereof of the invention, or an immunoconjugate comprising the antibody or antigen-binding fragment thereof of the invention or a pharmaceutical composition comprising the isolated monoclonal antibody or antigenbinding fragment thereof or the immunoconjugate of the invention.

The terms subject or patient are used interchangeably and refer to a subject that may benefit from the présent invention such as a mammal (e.g., canine, feline, ovine, porcine, equine, bovine, or human). In one spécifie embodiment the patient is human. Diagnosis of a disease or disorder associated with over-expression of MUC1 may be performed by a skilled physician by methods known in the art.

The term subject in need thereof' in the context of the présent invention inter alia refers to mammals, partîcularly human subjects sufferîng from a disease or disorder associated with over-expression ofMUCI, as defined herein.

It is to be understood that the terms treaf, “treating, “treatment' or forms thereof, as used herein, mean reducing, preventing, curing, reversing, ameîîorating, attenuating, alleviating, minimizing, suppressing, or halting the deleterious effects of a disease or a condition or delaying the onset of one or more clinical indications of a disease or disorder associated with over-expression of MUC1 (e.g., cancer), as defined herein. In some embodiments the methods according to the invention are wherein said methods further comprise administering to a subject in need thereof an additional therapeutic agent as herein defined.

Administration according to the présent invention may be performed by any of the following routes: oral administration, intravenous, intramuscular, intraperitoneal, intrathecal, or subcutaneous injection; intra-rectal administration; intranasal administration, ocular administration, or topical administration.

In spécifie embodiments administration according to the présent invention is performed intravenousiy.

The antibodies or antibody fragments as herein defined, any pharmaceutical compositions comprising the same or any conjugates comprising them may be administered to a subject in a single dose or in multiple doses.

A “therapeutically effective amount’ of the isoiated monoclonal antibody or any antigen binding fragment thereof according to the invention, or the pharmaceutical composition according to the invention for purposes herein defined is determined by such considérations as are known in the art to cure, arrest or at least alleviate or améliorais the medical condition. For any préparation used in the methods of the invention, the dosage or the therapeutically effective amount can be estimated initially from in vitro cell culture assays or based on suitable animal models.

In some embodiments the therapeutically effective amount in accordance with the invention is in the range of 10 pg/kg to about 50 mg/kg.

In other embodiments the therapeutically effective amount in accordance with the invention is in the range of 0.1 mg/kg to 40 mg/kg, 1 mg/kg to 10 mg/kg, or 5 mg/kg to 10 mg/kg.

Spécifie exemplary doses include, but are not limited to 0.25 mg/kg, or 0.75 mg/kg, or 2.5 mg/kg, or 5 mg/kg, or 10 mg/kg given as a daily dose, or once every three days, or once a week according to the physician's discrétion. In one embodiment, the doses are given intravenously.

The présent invention further provides the isoiated anti MUC1 SEA antibody or any antigenbinding fragment thereof according to the invention, or the immunocomplex according to the invention, or the pharmaceutical composition according to the invention for use in a method of treatment or amelioration of a disease or disorder assocîated with overexpression of MUC1 (e.g., cancer) as defined herein.

Still further the présent invention provides use of the isoiated monoclonal antibody or antigen-binding fragment thereof, the immunocomplex, or the pharmaceutical composition of the invention in the préparation of a médicament for the treatment or amelioration of a disease or disorder assocîated with over-expression of MUC1 (e.g., cancer), as defined herein.

It is appreciated that the tenu “purified” or isolated refers to molécules, such as amino acid or nucieic acid sequences, peptides, polypeptides, or antibodies that are removed from their natural environment, isolated, orseparaÏed. An “isolated antibody” istherefore a purified antibody. As used herein, the term ‘“purified' or to purifÿ also refers to the removal of contaminants from a sample.

In another aspect, the present invention provides a method of diagnosing a disease or disorder (e.g., cancer) in a biopsy obtained from a subject, said method comprising: a. contacting said biopsy with at least one isolated monoclonal antibody or antigenbinding fragment thereof of the invention; and b. detecfing said isolated monoclonal antibody or any antigen-binding fragment thereof;

wherein détection of cells over-expressing MUC1 SEA in the biopsy serves as an indication of said disease or disorder (e.g., cancer).

Assessment of the ability of the isolated antibodies of the present invention to detect MUC1SEA expression may be performed by any method known in the art, for example by immunohistochemistry or flow cytometry. Immunohîstochemistry may be performed on formaldéhyde fixed sections from Fresh Frozen (FF) tissues as wel! as on Paraffin Embedded and Formaldéhyde Fixed (PEFF) tissues. For example, as shown in Example 2 below, antibody DMB5F3stained MUC1-expressing cells present in both FF and PEFF sections. The antibody was also capable of recognizing MUC1-expressing cells using flow cytometry. In various embodiments the isolated antibodies in accordance with the present invention may be labeled according to any methods known in the art. In other embodiments détection may be based on identifying said antibodies using secondary antibodies.

The term “biopsy is used herein in its broadest sense and refers to any biopsy taken from a subject as herein defined in which cells overexpressing MUC1 SEA may be detected. Biopsies may be obtained from mammals (including humans) and encompass both fluid samples comprising cells, and tissue samples. in some embodiments the fluid sample is blood, plasma, sérum, lymph fluid or urine. In some embodiments the biopsy is a tissue sample suspected of containing cancerous cells.

a. introducîng at least one isolated anti-MUC1 SEA monoclonal antibody or antigenbinding fragment thereof of the invention into a subject, wherein said antibody or antigenbinding fragment thereof are detectably labelled with a radioisotope, or with a visualizable agent (Le., an agent that can be visualized, for example by scanning); and

The term about as used herein indicates values that may deviate up to 1%, more specifically 5%, more specifically 10%, more specifically 15%, and in some cases up to 20% higher or lower than the value referred to, the déviation range including înteger values, and, if applicable, non-integer values as well, constituting a continuons range. Disclosed and described, it is to be understood that this invention is not limited to the spécifie examples, methods steps, and compositions disclosed herein as such methods steps and compositions may vary somewhat. It is also to be understood that the terminology used herein is used for the purpose of describing spécifie embodiments only and not intended to be limiting since the scope of the présent invention will be limited only by the appended claims and équivalents thereof.

It must be noted that, as used in this spécification and the appended claims, the singular forms “a”, “an” and “the” include plural referents unless the content clearly dictâtes otherwise.

Throughout this spécification and the Examples and claims which follow, unless the context requires otherwise, the word “comprise, and variations such as “comprises” and “comprising”, will be understood to imply the inclusion of a stated integer or step or group of integers or steps but not the exclusion of any other integer or step or group of integers or steps.

EXAMPLES

Materials and Methods

Reagents and antibodies

Ail reagents and Chemicals were obtained from Sigma (St. Louis, MO), unless otherwise specified. Secondary antibodies used in cell counter-staining or in immunohistochemical development were obtained from Jackson ImmunoResearch Laboratories (Bar Harbor, ME).

Immunization of mice and génération of hybridomas

Mice were in itially immunized with 5 consecutive intradermal DNA immunizations spaced at 21-day intervals. The immunizing DNA consisted of the pCL-MUC1-TM expression vector plasmîd that codes for the MUC1-TM protein. The extracellular domain of MUC1-X protein (recombinant bacterial MUC1-Xex) together with incomplète Freund's adjuvant was then used to boost the mice. Bacterially synthesized recombinant MUC1-Xex protein used for these immunizations spontaneously self-cleaves, generating the MUC1-X a and b subunits that strongly, yet noncovalently, interact with each other forming the very stable heterodimeric cleaved MUC1-Xex protein. Hybridomas were prepared by fusion of nonsecreting myeloma cells with immune splénocytes and screened by ELISA assay.

ELISA for determining binding of anti-MUC1 polyclonal and monoclonal antibodies to the extracellular domain of the MU Cl -X protein

Elisa Immunoassay plates (CoStar) were coated with recombinant MUC1 proteins followed by blocking. Spent culture media from the initial hybridomas was then applied to the wells. Following incubation, samples were removed, and the wells were washed with PBS/Tween. Détection of bound antibodies was done with horseradish peroxidase (HRP)-conjugated anti-mouse antibody.

Two-tiered screening for sélection of anti-MUC1 monoclonal antibodies

The primary screen of the hybridomas was carried out by assessing antibody binding to the extracellular domain of MUC1-X (MUCLXex) as described in the ELISA assay above. To select hybridomas secreting antibody that recognize not only MUC1-Xex but also the complété cell surface MUC1-TM protein, those hybridomas presenting a positive signal in the first screen were subjected to a second-tier screen. This consisted of flow cytométrie analysis using mouse cell transfectants (termed DA3-TM cells) which express human MUC1-TM and, in parallel, the same parental cells (termed DA3-PAR ceiis) that do not express human MUC1. This procedure ensured sélection of antibody that not only bound MUC1 moîeties common to both the MUC1-X and MUC1-TM but also recognized the cell surface human MUC1-TM molécule as it is expressed by mammalian cells.

Cell fines and cell cultures

DA3-PAR parental mouse mammary cells (which do not express human MUC1 ), DA3-TM mouse mammary cells stably transfected with cDNA (and encoding for the full-length human MUC1-TM), cell lines T47D and ZR75 (human breast carcinomas), cell fines KB (human epidermoid carcinoma), Colo357 (human pancreatic carcinoma), N87 (human gastric carcinoma), and CHO-K1 (Chinese hamster ovary cells) were ail grown in Dulbecco's Modified Eagle's Medium (DMEM), RPMI, and DMEM:F12 (1:1) culture media [12].

Animais

Seven-weeks-old athymie (nude) and SCID mice (Harlan Laboratories, Madison, Wl) were maintained until sacrifice in facilities approved by the Tel Aviv University (TAU) Institutiona! Ethics Committee for Accréditation of Laboratory Animal Care, in accordance with the régulations and standards of the Israël Ministry of Health.

Flow cytometry analysis

Following trypsinization, MUC1-expressing tumor cells were washed and incubated with DMB5F3 (0.5 pg/ml), with or without MUC1-Xex competitor (100 pg/ml), for 1 hr at 4°C. After washing with FACS buffer, fluorescein-labeled goat anti-mouse IgG was added for 45 min at 4°C. Bound IgG was detected by flow cytometry on a FACSCalibur™ (Becton Dickinson),

Immunohistochemical (IHC) staining

Microarrays of normal and malignant pancreatic and breast tissue were purchased from US Biomax (Derwood, MD). Automated Immunological stains were obtaîned with the use of the Dako Autostainer Link 48 (Dako, Santa Clara, CA), according to the manufacturées instructions. Antigenic retrieval was carried out with citrate buffer, for 30 min at room température. Endogenous peroxidase activity was blocked by the addition of Envision Flex Peroxidase Blocking Reagent (Dako) for 30 min, followed by incubation with DMB5F3 (5 pg/ml) for 2hrs. The immunohistochemical reaction was detected by the addition of polymer dextran coupled with peroxidase and secondary antibodies for 15 min (EnVision-Flex/HRP, Dako) and diaminobenzidine for 10 min (DakoCytomation). This was followed by counterstaining with hematoxylin for 10 min.

Sequence détermination of anti-MUC1-SEA module monoclonal antibodies

RNA was isolated from the DMB hybridoma sériés with TRIzol© Reagent I, according to a technical manual for the reagent (Ambion Inc., Foster City, CA). The RNA sequence was determined as follows: cDNA was generated by reverse transcription of total RNA with the use of universal or isotype-specific anti-sense primers, according to the technical manual for PrimeScrîptTM 1st Strand cDNA Synthesis Kit (Takara Bio Inc., Mountain View, CA). Amplification of VH and VL antibody fragments was carried out according to the standard operating procedure (GenScript, NJ, USA) which involves rapid amplification of cDNA ends, followed by separate cloning into a standard cloning vector. Clones with inserts of the correct sizes were sequenced by colony PCR and at least five colonies with such inserts were sequenced for each fragment, with the consensus sequence derived by alignment of the different clones.

Construction of chimeric chDMB5F3 for mammalian expression in Chinese hamster ovary cells

Human chimeric DMB5F3 (chDMB5F3) was generated from mouse DMB5F3. The mammalian vectors pMAZ-IgH and pMAZ-lgL were used as backbones for expression of cDNA coding for the VH and VL réglons of DM85F3 fused to human yl heavy and human k light chains, respectively [Mazor et al., J Immunol Methods, 321 (2007) 41-59; Mazor et al., Cancer Lett, 257 (2007) 124-135], The generated pMAZ lgH-chDMB5F3 and pMAZ lgL-chDMB5F3 vectors were used for transfection, and the résultant chimeric antibody chDMB5F3 was expressed in CHO cells. Stably transfected CHO cells secreted chDMB5F3, which was purified by protein-A affinity chromatography.

Préparation ofthe chDMB5F3: ZZ-PE38 immunocomplex

The génération of the chDMB5F3:ZZ-PE38 immunocomplex was carried out as described in Pichinuk et al [11]. Briefly, chDMB5F3 was mixed with purified recombinant ZZ-PE38 protein in 20 mM Hepes buffer at a 2-fold molar excess of ZZ-PE38 and incubated for 2 hours at 4°C. Excess ZZ-PE38 and unconjugated chDMB5F3 antibody were removed by passage through a Sephadex G200 sizing coiumn.

In vitro cell viability assay

T47D, KB, A431 and N87 cancer cells (20,000 cells/well) were seeded in 96-wel! cell culture plates and grown at 37°C in 5% CO2. After seeding, the chDMB5F3: ZZ-PE38 immunocomplex was applied directly to the cells at a concentration of 100ng/ml. Négative Controls consisted of target cells reacted with ZZ-PE38 toxin alone, unconjugated to chDMB5F3 antibody, or with chDMB5F3 monoclonal antibody alone, devoid of ZZ-PE38 toxin. Cell viability was assessed according to alkaline phosphatase activity/well. The results were calculated as the average of 2 to 3 experiments, performed in tripiicate.

ELISA for détermination of the binding of chDMB5F3: ZZ-PE38 immunocomplex to ML)C1-Xex protein

To quantitate chDMB5F3 levels in mouse sérum, ELISA immunoassay plates were coated with recombinant MUC1-Xex protein (see Fig. 1C showing the schematic structure), followed by blocking. At Days 1,7, 15 and 28 after a single dose of 5 pg chDMB5F3: ZZPE38 immunocomplex, the mouse sera were applied to the ELISA wells at doubiing dilutions and bound antibody was detected with horseradish peroxidase-conjugated goat anti-human Fc antibody. The results were caiculated as the average of 2 to 3 experiments, performed in tripiicate.

In vivo cytotoxicity assay

Two quantitatively measurable human tumor xenograft models were established, one in 7weeks-oid femaie athymie nude mice and one in 7-weeks-old SCID mice, by using Colo357, a MUC1⁺ human pancreatic cancer cell line. A total 3x10⁶ Colo357 cells suspended in a small volume (100 pl) of Hepes buffer were înjected subcutaneously into the right flank of the mice. In both the nude and the SCID mouse studies, the mice were divided into three groups (5 mice/group): Group 1 received 5 pg chDMB5F3: ZZ-PE38 (0.25mg/kg), Group 2 received 5 pg non-specific human Ig: ZZ-PE38 (0.25mg/kg) and Group 3 received an équivalent volume of Hepes buffer alone. In the athymie nude mice (7-weeks-old female mice), administration of anti-MUC1 immunotoxin, non-specific immunotoxin or Hepes in the three experimental groups was initiated 24 hours after the injection of Colo357 cells. The injection protocol consisted of a total six intravenous (iv) administrations on Days 1, 6, 9, 15, 22 and 29 in each experimental group (see black arrows along x-axis, Figure 6). In the SCID mice (7-weeks-old female mice), chDMB5F3: ZZ-PE38, non-specific human Ig: ZZ-PE38, and Hepes were administered similarly starting 24 hours after injection of pancreatic tumor cells in each of the three groups. The overall protocol of administrations consisted of eight iv injections on Days 1, 4, 8, 11, 16, 24, 31 and 38 in each ofthe three groups. Tumor growth was assessed serially in each ofthe experimental groups with a digital caliper. Tumor volume was calculated according to the formula 0.5 x L x W², where L is tumor length, and W- tumor width as described in Tomayko and Reynolds (Cancer Chemother Pharmacol, 24 (1989) 148-154). Ail the animal experiments were approved by the TAU’s Instîtutional Review Board.

Statistics

Statistical analysis of in vivo tumor growth was performed according to a simple paired 1taüed t test, A p value of less than 0.05 was considered statistically significant.

Results

Example 1: Génération and sequencing of the DMB mAbs that bind the cell-bound MUC1 α-β junction and characterization ofthe DMB5F3 mAb

Anti-MUC1 monoclonal IgGs were generated from hybridomas produced with spleen cells isoiated from inoculated mice having high titers of polyclonal anti-MUC1-Xex antibody, The MUC1-Xex recombinant protein used for immunization, and its relationship to the transmembrane MUC1-TM and MUC1-Xex proteins is shown in Fig. 1 (compare 1C with 1B and 1 A). A total of seven DMB mAbs were thus generated,

The nucléotide sequences of the variable régions of ail the anti-MUC1 SEA α-β junction monoclonal antibodies were determined as described above in Materials and Methods, and the deduced amino acid sequences ofthe mAbs are presented in Figure 2. Sequencing of the résultant mAbs showed that they clustered into 4 groups, denominated [I] DMB5F3^!I], [II] DMB7F3W [III] DMB4B41^lal and DMB10F10 and [IV] DMB4F4t^|V-^al, DMB10B7t^|Vbl and DMB13D11^|lv’^cl(see Fig. 2 and Tables 1 and 2 for full nucléotide sequence and amino acid sequence). Sequences within each group revealed either unique mAb sequences as for DMB5F3^m and DMB7F3^[ll! or mAbs with identical Vu and Vl sequences, as for Groups [III] and [IV],

The variable domains for ail antibodies are typical of affinity maturation as expected from antibodies generated by prime-boost. AH mAbs except for group [IV] mAbs DMB10B7^EIV ^bl and DMB13D11^[IV'^C1 were Ig-gamma1. Group [IV] contained three mAbs with îdentical Vh and V_L sequences- one (DMB4F4^[lv‘^a]) was of the Ig-gamma1 subtype, whereas the remaining two (DMB10B7i^|V-^b1 and DMB13D11^[IV-^C1), were IgA.

AH seven anti-MUOI α-β junction mAbs robustly bound to cells expressing the transmembrane MUC1-TM protein, as assessed by fiow cytometry (Fig. 1D-1K). Représentative mAbs from each ofthefour mAb groups were also assessed for their ability to detect MUC1-TM expression by immunohistochemistry performed on formaldéhyde fixed sections from Fresh Frozen (FF) tissues as well as on Paraffin Embedded and Formaldéhyde Fixed (PEFF) tissues. Antibody DMB5F3^[IJ stained MUC1-expressing cells present in both FF and PEFF sections (see below), whereas the DMB7F3¹ ¹ and mAbs from group [IV] stained only on FF sections- in contrast, group [III] mAbs while binding well to MUC1-expressing cells as assessed by flow cytometry, were non-reactive with both FF and PEFF sections (data not shown).

The VH domain is derived from mouse germline V gene IGHV3-1*02 with 9 somatic mutations, it is 105/122(86%) îdentical to the highest scoring îdentical sequence in an NCBI BlastP search. The VL domain (V-kappa) is derived from mouse germline V gene IGKV548*01 with 3 somatic mutations. It is 101/107(94%) îdentical to the highest scoring îdentical sequence in an NCBI BlastP search.

Flow cytometry analyses showed that DMB5F3 bound strongly to DA3 cells stably transfected with full length MUC1 (DA3-TM) (Fig. 1D), whereas non-transfected DA3-PAR cells, which do not express MUC1, were consistently négative (Fig. 1E). Colo357, a MUC1positive human pancreatic cancer cell line, as well as the MUC1 positive breast cancer cell lines T47D and ZR75, exhibited strong reactivity with DMB5F3 (Fig. 1D, 1F, 1H and 1J), The addition of competing soluble recombinant MUC1-Xex protein (see Fig. 1C for the structure of recombinant MUC1-Xex), abolished ail DMB5F3 cell binding (Fig. 1G, 11 and 1K), confirming antibody specificity.

Example 2: IHC staining of human pancreatic and breast tissue sections with DMB5F3

To détermine the degree to which monoclonal DMB5F3 binds malignant and normal tissues, a variety of malignancies in tissue microarrays, including breast, pancreatic, lung, prostate, and colon carcinomas underwent immunohistochemical staining (exemplary results are shown in Figures 3 and 4). The rationale to do extensive analyses for MUC1 expression, despite previous reports demonstrating MUC1 overexpression in maiignancy lies in the significant fact that the anti-MUC1 mAbs described here recognize the MUC1 SEA module and were generated by immunization with recombinant MUC1-Xex protein (see Fig. 1C), and not with the MUC1-TM protein (see Fig. 1A). Analyses of MUC1 expression to date hâve almost exclusively been done with antibodies recognizing epitopes within the a-chain VNTR moiety. It was thus intriguing to analyze for the first time the cell récognition patterns of anti-MUC1-SEA domain antibodies. The tissue microarrays used for these analyses included the following (Biomax microarray désignation in parenthesis): 6 pancreatic tumors with adjacent non-neoplastic tissues (PA241); 40 different pancreatic tumors and 8 normal pancreatic tissues (PA483); 3 samples each of breast plasma cell mastitis, adenosis and fibroadenoma and 36 invasive breast ductal carcinomas plus 2 invasive breast iobular carcinomas (BR963a) and 10 cases each of colon, breast, prostate, lung and coion carcinoma, in addition to two sections each from the corresponding normal tissues (TP481). A représentative composite array of normal and malignant tissues immunohistochemically stained with DMB5F3 is shown in Fig. 3. Of the 46 pancreatic tumors (in microarrays PA241 and PA483), 44 exhibited strong reactivity with DMB5F3; tumor cells stained în a near-circumferential pattern (for examples see Fig. 3C and 3D). In contrast, normal pancreatic tissue DMB5F3 reactivity was restricted to the luminal surface of the ductal pancreatic épithélial cells (Fig. 3A).

Of the breast tissue samples analyzed on the BR963a microarray, minimal to no staining was seen in non-malignant tissues that included normal breast tissue, plasma cell mastitis, adenosis and fibroadenoma. !n contrast, 21 of the 36 invasive breast ductal carcinomas showed very high DMB5F3 reactivity, 4 showed iow levels of expression, and 11 samples showed little to no expression. The examined pancreatic tissues included both acinar (Fig. 3D) and ductal adenocarcinomas (Fig. 3C and Fig. 4N and 40), and malignant breast tissues in the microarray consisted of invasive ductal carcinomas (Fig. 3G and 3H). Malignant cells from pancreatic carcinomas (Fig. 3C and 3D and Fig. 4N and 40), breast carcinomas (Fig. 3G and 3H, and Fig. 3I-3N, patients 1-6) and lung, prostate and colon carcinomas (Fig. 4A, 4D and 4G, respectively, Figures 4B, 4E and 4H at higher magnifications) were strongly reactive with DMB5F3, with near-circumferential cellular staining. In contrast, normal pancreatic acinar cells showed only weak apical positivity (indicated by black arrows in Fig. 3A(i); higher magnification is shown in Fig. 3B, and Fig. 4M), consistent with a previous description [13], Normal breast ductal épithélial cells (Fig. 3E and 3F) and normal gland-like structures formed by non-malignant épithélial cells adjacent to the malignancy on the microarray exhibited weak apical positivity (Fig. 3I-3N présent biopsy sections from six patients; normal gland-like structures are indicated by black arrows). This was in marked contrast to malignant cells in the same section that stained strongly with DMB5F3 anti-MUC1-SEA antibody (Fig. 3I-3N). As malignant and non-malignant material were on the same microarray sample and, therefore simultaneously and uniformly stained, the possibility that mere technical différences in handling and staining could account for the findings may be excluded. Moreover, the fact that soluble MUC1-Xex competed-out cell staining by DMB5F3 confirmed the anti-MUC1 specificity of DMB5F3 (compare Fig. 3A and 3B, respectively).

To extend these observations to other tumor types, lung, prostate, and colon carcinomas were examined immunohistochemically with DMB5F3 (Fig. 4A, 4D and 4G). The results showed a similar pattern of MUC1 distribution to that observed in breast and pancreatic carcinomas (Fig 3). In addition to the increased density of MUC1 expression at the cellular level, distinctions in MUC1 architecture were also observed: Anti-MUC 1-SEA DMB5F3 bound malignant cells in a near-circumferential pattern. Here too, staining with DMB5F3 was abolished in the presence of competing soluble MUC1-Xex protein, attesting to the specificity of DMB5F3 (data not shown) and no staining was observed with non-immune mouse immunoglobulin (compare Fig. 4A, 4D, 4G and 4J with 4C, 4F, 4I and 4L). The majority of the nearly 50 lung, prostate, colon, breast, and pancreatic adenocarcinoma tissues examined in the microarrays showed a similar IHC staining pattern, a minority expressing lower amounts of MUC1 and a few having negligible MUC1 expression. This non-uniformity is consistent with the heterogeneity of tumor phenotypes in general and of MUC1 particularly. Because pancreatic carcinoma, a high-mortality MUC1 expressing malignancy was selected for in vivo studies (see below), pancreatic tumor tissue from an additional sériés of patients was examined to confirm the altered tumor-associated architecture of MUC1 expression (see représentative staining in Fig. 4N and 40). Although these analyses reveal increased circumferential DMB5F3 immunoreactivity over the entire cell surface of the adenocarcinoma cancer cells, the following two caveats are pertinent: (a) immunohistological analyses are only semi-quantitative and (b) in some cases MUC1 is strongly expressed within the cell, rendering comparisons of surface expression difficult. Notwithstanding these two provisos, cancer cells derived from adenocarcinomas clearly exhibit high ceil-surface immunoreactivity with DMB5F3.

Example 3: In vitro cytotoxicity of the chDMB5F3; ZZ-PE38 immunocomplex

After demonstrating the reactivity of DMB5F3 with cancer cells expressing cell surface MUC1 both in cell lines (Fig 1D-1K) and in microarrays of tissue biopsies (Fig. 3 and 4), the antibody’s ability to ferry a cytotoxîc moiety into malignant cells was examined. The ZZPE38 fusion protein consists of Pseudomonas exotoxin PE38 and the IgG-binding ZZdomain derived from Protein A. Because the ZZ domain binds tightly to human Fc and shows negligible binding to mouse lgG1 Fc, a chimeric DMB5F3 antibody was generated in which mouse IgG 1-Fc was substituted for human Fc and then ZZ-PE38 added to the chimeric (ch)DM5F3 to form the immuno-toxin complex, as described in Materials and Methods.

As the ZZ-PE38 toxin aione is unable to bind to or internalize into cells, ail tumor cytotoxicity induced by the DMB5F3-ZZ-P38- immunocomplex is due solely to cell binding and internalization by the anti-MUC1 DMB5F3 immunocomplex [Mazor et aL, J Immunol Methods, 321 (2007) 41-59; Mazor et al., Cancer Lett, 257 (2007) 124-135], The cell lines T47D, KB, A431 and N87 were cytometrically analyzed with chDMB5F3, Erbitux and Herceptin at concentrations of 300ng/ml. MUC1⁺ T47D cells (breast carcinoma) and KB cells (epidermoid tumors) were found to be sensitive to chDMB5F3: ZZ-PE38 immunocomplex-mediated inhibition of cell growth, with tumor cytotoxicity seen at antibody concentrations as low as 200pM (Fig. 5A and 5B. In contrast, T47D breast cancer cells expressing low, yet clearly détectable, levels of EGFR1 were insensitive to Erbitux®: ZZPE38 (Fig. 5A, diamond shape tracing), and only partîally sensitive to Herceptin®: ZZ-PE38 (Fig. 5A, rectangle tracing). KB cells, which expressed low, yet clearly détectable levels of erbB2-EGFR2, were insensitive to Herceptin®: ZZ-PE38 (Fig. 5B, rectangle tracing). Cells expressing markedly lower levels of MUC1 such as N87 showed approximately 40% cytotoxicity, contrasting with the high MUC1 expression and high cytotoxicity seen in T47D and KB cells (compare Fig. 5D, with Fig. 5A and 5B). A431, the lowest MUC1 expresserof ail cell lines investigated, contained a major population of cells that showed no MUC1 expression with only a much smaller subpopuiation expressing low-level MUC1. Consistent with this low level of MUC1 expression chDMB5F3: ZZ-PE38 immunocomplex resulted in a very limited cytotoxicity of A431. These results indicate that a threshoid level of cell surface MUC1 expression and density is a necessary requirement to elicit cytotoxicity. A similar phenomenon is observed with the absence of cytotoxicity of Herceptinimmunotoxin-complex when applied to KB cells despite low, yet détectable, levels of erbB2EGFR2 expression by these cells (Fig. 5B), and Erbitux-immunotoxin-complex when applied to T47D cells that also express low, yet détectable, levels of EGFR1 (Fig. 5A).

Exampie 4: Pharmacokinetics of chDMB5F3 in nude mîce

The in vivo stability of chDMB5F3 was evaluated by assessing sérum levels on Days 1,7, 14 and 28 following iv administration. The results showed that compared with Day 7, sérum levels of antibody chDMB5F3 on Days 14 and 28 were twofold and fourfold decreased, respectively (Fig. 6D), consistent with previously reported half-lives in mice of in vitro generated chimeric IgGs and of antibodies in clinical use. As for the toxin conjugate, the half-life of Pseudomonas exotoxin has been shown to be extended by îinkage to IgG.

Example 5: In vivo cytotoxicity of chDMB5F3: ZZ-PE38 immunocomplex in xenotransplanted human tumors

Administration of the chDMB5F3:ZZPE38 immunocomplex to nude mice xenotransplanted with MUC1⁺ human pancreatic Colo357 cells resulted in a marked cytocidal effect with a réduction in tumor volume at Days 21, 28 and 35 versus that in the control groups, which had received Hepes buffer or nonspecific, isotype-matched IgG-ZZ: PE38 (Fig. 6A). Upon completion of chDMB5F3:ZZPE38 immunocomplex administration, the tumor volume of the treated group, as expected, încreased progresstvely in parallel with that in the control groups and by Day 40 (when the animais were sacrificed) the tumor volume in ail three groups reached the 200-400 mm³ range (Fig. 6A). This again confirmed the tumorsuppressive effect of administered chDMB5F3:ZZPE38.

A factor conceivably limiting the cytotoxic efficacy of the chDMB5F3:ZZPE38 immunocomplex in xenotransplanted nude mice is endogenous circulating antibody, which by inieracting with the ZZ linker may at least partially displace ZZ-PE38 toxin from chDMB5F3. Although ZZ does not bind mouse IgG 1, it can bind mouse lgG2. Limitation in immunotoxin efficacy by displacement does not reflect defective bînding of antibody chDMB5F3 to tumor cell surface MUC1 but arises from toxin loss owing to reduced ZZmediated linkage of ZZ-PE38 to the chDMB5F3 antibody. To avoid this complicating factor, a nearly identicai study in SCID mice, which lack détectable endogenous antibody, was then performed. As in the nude mouse protocol, transplanted SCID mice were divided into three groups, one receiving the chDMB5F3:ZZPE38 immunocomplex, one receiving a matched-isotype lgG-ZZ:PE38, and one receiving Hepes buffer alone, each initiated 24 hours after injection of pancreatic tumor. As noted in the Materials Methods section, the protocol consisted of serial administrations in each group on Days 1, 4, 8, 11, 16, 24, 31, and 38. The chDMB5F3: ZZ-PE38 immunocomplex exhibited a marked antitumor effect: In SCID mice treated with chDMB5F3: ZZ-PE38 the volume of xenotransplanted Colo357 human tumors was reduced by as much as 90% versus that in the control groups (Figure 7). The actual values of post-treatment tumor volumes (in mm³) are as follows: Group 1 mice treated with the chDMB5F3-immunotoxin: 2, 14, 16, 25 and 36 mm³; Group 2 mice treated with the non-specific, isotype-matched lgG-ZZ:PE38: 180, 225, 258 and 270 mm³(lack of tumor take for one mouse in this group is not included); and Group 3 mice treated with Hepes buffer alone: 180, 245, 304, 705 and 1008mm3. The extended scheduling of chDMB5F3: ZZ-PE38 administration to Days 31 and 38 assured an anti-tumor effect as late as Day 49.

Example 6: Sequencing of DMC209

Total RNA was isolated from hybridoma cells following the technical manual of RNeasy Pius Micro Kit (QIAGEN, Cat. No.: 74034). Total RNA was then reverse-transcribed into cDNA using either isotype-specific anti-sense primers or universal primers following the technical manual of SMARTScribe Reverse Transcriptase (TaKaRa, Cat. No.: 639536).

Antibody fragments of heavy chain and light chain were amplified according to the standard operating procedure (SOP) of rapid amplification of cDNA ends (RACE) of GenScript. Amplified antibody fragments were cloned into a standard cloning vector separately. Colony PCR was performed to screen for clones with inserts of correct sizes. The 5 consensus sequence is provided in Table 1 (for the heavy chain variable région SEQ ID

NO:11 and for the light chain variable région SEQ ID NO: 12),

Claims

1. An isolated monoclonal antibody or antigen-binding fragment thereof which binds to the MUC1 SEA domain, wherein said antibody comprises:

2. The isolated monoclonal antibody according to claim 1, wherein said antibody comprises a heavy chain variable région and a light chain variable région, wherein:

a. said heavy chain variable région is encoded by a nucieic acid sequence which is at least 70% identical to the nucieic acid sequence denoted by SEQ ID NO. 1 and wherein said light chain variable région is encoded by a nucieic acid sequence which is at least 70% identical to SEQ ID NO. 2; or

b. said heavy chain variable région is encoded by a nucieic acid sequence which is at least 70% identical to the nucleic acid sequence denoted by SEQ ID NO. 3 and wherein said light chain variable région is encoded by a nucleic acid sequence which is at least 70% identical to SEQ ID NO. 4; or

c. said heavy chain variable région is encoded by a nucleic acid sequence which is at least70% identical tothe nucleic acid sequence denoted by SEQ ID NO. 5 and wherein said light chain variable région is encoded by a nucleic acid sequence which is at least 70% identical to SEQ ID NO. 6; or

3. The isolated monoclonal antibody according to claim 1 or claim 2, wherein said antibody comprises:

a. a heavy chain variable région comprising the amino acid sequence denoted by

SEQ ID NO. 13 or a variant thereof and a light chain variable région comprising the amino acid sequence denoted by SEQ ID NO. 14, or a variant thereof; or

b. a heavy chain variable région comprising the amino acid sequence denoted by

SEQ ID NO. 15 or a variant thereof and a light chain variable région comprising the amino acid sequence denoted by SEQ ID NO. 16, or a variant thereof; or

c. a heavy chain variable région comprising the amino acid sequence denoted by

SEQ ID NO. 17 or a variant thereof and a light chain variable région comprising the amino acid sequence denoted by SEQ ID NO. 18, or a variant thereof; or

d. a heavy chain variable région comprising the amino acid sequence denoted by

SEQ ID NO. 19 or a variant thereof and a light chain variable région comprising the amino acid sequence denoted by SEQ ID NO. 20, or a variant thereof; or

e. a heavy chain variable région comprising the amino acid sequence denoted by SEQ ID NO. 21 or a variant thereof and a light chain variable région comprising the amino acid sequence denoted by SEQ ID NO. 22, or a variant thereof; or

f. a heavy chain variable région comprising the amino acid sequence denoted by

SEQ ID NO. 23 or a variant thereof and a light chain variable région comprising the amino acid sequence denoted by SEQ ID NO. 24, or a variant thereof.

4. The isolated monoclonal antibody or antigen-binding fragment thereof according to any one of the preceding claims, wherein said antibody is a mouse antibody, a chimeric antibody, a humanized antibody, or a human antibody.

5. The isolated monoclonal antibody or antigen-binding fragment thereof according to any one ofthe preceding claims, wherein said antigen-binding fragmentthereof is Fv, single chain Fv (scFv), single chain Fv-Fc (scFv-Fc), Fab’, Fab, F(ab’)₂ or F(ab)₂.

6. The isolated monoclonal antibody or antigen-binding fragment thereof according to any one of the preceding claims, wherein said antibody or antigen-binding fragment thereof identify MUC1 SEA in immunohistochemistry performed on formaldehyde fixed sections from Fresh Frozen (FF) tissues, and/or on Paraffin Embedded and Formaldehyde Fixed (PEFF) tissues, and/or by fluorescence-activated cell sorting (FACS) analysis of MUC1expressing human cells.

7. An isolated nucleic acid molécule comprising a nucléotide sequence encoding an antibody or any antigen-binding fragment thereof according to any one of claims 1-6.

8. An expression vector comprising the isolated nucleic acid molécule according to claim 7.

9. A host cell transfected with the expression vector according to claim 8.

10. An immunoconjugate comprising the antibody or antigen-binding fragment thereof according to any one of claims 1-6 and an additional cytotoxic or therapeutic agent.

11. The immunoconjugate according to claim 10 wherein said cytotoxic agent is selected from a group consisting of alkylating drugs, anthracyclines, pyrimidine dérivatives, vinca alkaloids, photodynamic drugs, platinum-containing compounds, taxanes, topoisomerase inhibitors, ribosome inactivating agents, agents that induce DNA damage, tubulin inhibitors, anti-mitotic agents, radioisotopes, cytotoxic antibodies and bacterial toxins.

12. The immunoconjugate according to claim 10 wherein said cytotoxic agent is pseudomonas exotoxin.

13. The immunoconjugate according to any one of claims 10 to 12, wherein said immunoconjugate reduces tumor volume upon administration to a subject with cancer,

14. A bispecific antibody comprising the antibody or antigen-binding fragment thereof according to any one of daims 1-6 and a second antibody binding a different antigen target.

15. A pharmaceutical composition comprising as an active ingrédient the isoiated monoclonal antibody or antigen-binding fragment thereof according to any one of daims 16, or immunoconjugate according to any one of daims 10 to 13, or the bispecific antibody of claim 14 and a pharmaceutically acceptable carrier, excipient, or diluent.

16. The pharmaceutical composition according to daim 15, wherein said pharmaceutical composition further comprises an additional therapeutic agent,

17. The use of at least one isoiated monoclonal antibody or antigen-binding fragment thereof according to any one of daims 1-6, or the immunoconjugate according to any one of daims 10 to 13, or the bispecific antibody of claim 14in the manufacture of a médicament for use in a method of treatment, amelioration, diagnosing or imaging of a disease or disorder, such as cancer, an autoimmune or inflammatory disease or a non-malignant clinically significant abnormal growth condition.

18. The isoiated monoclonal antibody or antigen-binding fragment thereof according to any one of daims 1-6, the immunoconjugate according to any one of daims 10 to 13, the bispecific antibody ofclaim 14, orthe pharmaceutical composition according to claim 15 or 16 for use in a method of treatment or amelioration of a disease or disorder, said method comprising administering to a subject in need thereof a therapeutically effective amount of said isoiated monoclonal antibody or antigen-binding fragment thereof, said immunoconjugate or said pharmaceutical composition.

19. The isoiated monoclonal antibody or antigen-binding fragment thereof, or immunoconjugate, or bispecific antibody, or pharmaceutical composition for use according to daim 18, wherein said disease or disorder is cancer.

20. The isoiated monoclonal antibody or antigen-binding fragment thereof, or immunoconjugate, or bispecific antibody, or pharmaceutical composition for use according to claim 18, wherein said disease or disorder is an autoimmune or an inflammatory disease, or wherein said disease or disorder is a non-malignant abnormal growth condition, e.g,, cysts, for example renal cysts, thyroid cysts and thyroid masses, or hepatic cysts.

21. The isoiated monoclonal antibody or antigen-binding fragment thereof, or immunoconjugate, or bispecific antibody or pharmaceutical composition for use according to any one of claims 18-20, wherein said method further comprises administering to a subject in need thereof an additional therapeutic agent.

22. A method of diagnosing a disease or disorder in a subject, wherein said disease or disorder is associated with MUC-1 expression, said method comprising:

5 a. contacting a biopsy obtained from said patient with at least one isolated monoclonal antibody or antigen-binding fragment thereof according to any one of claims 1 6; and

b. detecting said isolated monoclonai antibody or any antigen-binding fragment thereof;

10 wherein détection of cells over-expressing MUC1 SEA in the biopsy indicates that the subject is dtagnosed with said disease or disorder.

23. The method according to claim 22, wherein said disease or disorder is cancer.

24. The method according to claim 22, wherein said disease or disorder is an autoimmune or an inflammatory disease, or wherein said disease or disorder is a non15 malignant abnormal growth condition, e.g., cysts, for example rénal cysts, thyroid cysts and thyroid masses, or hepatic cysts.

25. The method of any one of claims 22-24, wherein said isolated monoclonal antibody or antigen-binding fragment thereof is detectabiy labelled.