OA17903A

OA17903A - Prodrugs of pyridone amides useful as modulators of sodium channels.

Info

Publication number: OA17903A
Application number: OA1201600213
Authority: OA
Inventors: Corey Anderson; Sara Sabina Hadida-Ruah; Julian Marian Charles GOLEC; Beili Zhang; Benjamin Joseph Littler; Ali Keshavarz-Shokri; Tim Edward Alcacio; Daniel T. Belmont
Original assignee: Vertex Pharmaceuticals Incorporated
Priority date: 2013-12-13
Filing date: 2014-12-12
Publication date: 2018-02-27

Abstract

The invention relates to prodrug compounds of formula I : wherein R2 , R3 , R5 , R7 and X are as defined herein. The invention also provides pharmaceutically acceptable compositions comprising the compounds of the invention and methods of using the compositions in the treatment of various disorders, including pain. The compounds of formula I possess advantageous solubility and physicochemical properties.

Description

Pain is a protective mechanism that allows healthy animais to avoid tissue damage and to prevent further damage to injured tissue. Nonetheless there are many conditions where pain persists beyond its usefulness, or where patients would benefit from inhibition of pain. Neuropathie pain is a form of 10 chronic pain caused by an injury to the sensory nerves (Dieleman, J.P., et al., Incidence rates and treatment of neuropathie pain conditions in the general population. Pain, 2008.137(3): p. 681-8). Neuropathie pain can be divided into two categories, pain caused by generalized metabolic damage to the nerve and pain caused by a discrète nerve injury. The metabolic neuropathies include post herpetic neuropathy, diabetic neuropathy, and drug-induced neuropathy. Discrète nerve injuries indications include post amputation pain, post-surgical nerve injury pain, and nerve entrapment injuries like neuropathie back pain.

Voltage-gated sodium channels (Na_v’s) play a critical rôle in pain signaling. Na_v’s are key biological mediators of electrical signaling as they are the primary mediators of the rapid upstroke of the action potential of many excitable cell types (e.g. neurons, skeletal myocytes, cardiac myocytes). The evidence 20 for the rôle of these channels in normal physiology, the pathological states arising from mutations in sodium channel genes, preclinical work in animal models, and the clinical pharmacology of known sodium channel modulating agents ail point to the central rôle of Na_v’s in pain sensation (Rush, A.M. and T.R. Cummins, Painful Research: Identification of a Small-Molecule Inhibitor that Selectively Targets Na_vl.8 Sodium Channels. Mol Interv, 2007. 7(4): p. 192-5); England, S., Voltage-gated sodium 25 channels: the search for subtype-selective analgésies. Expert Opin InvestigDrugs 17 (12), p. 1849-64 (2008); Krafte, D. S. and Bannon, A. W., Sodium channels and nociception: recent concepts and therapeutic opportunities. Curr Opin Pharmacol 8 (1), p. 50-56 (2008)). Nav’s are the primary mediators of the rapid upstroke of the action potential of many excitable cell types (e.g. neurons, skeletal myocytes, cardiac myocytes), and thus are critical for the initiation of signaling in those cells (Hille, Bertil, Ion 30 Channels of Excitable Membranes, Third ed. (Sinauer Associâtes, Inc., Sunderland, MA, 2001)).

Because of the rôle Na_v’s play in the initiation and propagation of neuronal signais, antagoniste that reduce Nay currents can prevent or reduce neural signaling and Na_v channels hâve long been considered likely targets to reduce pain in conditions where hyper-excitability is observed (Chahine, M., Chatelier, A., Babich, O., and Krupp, J. J., Voltage-gated sodium channels in neurological disorders. CNSNeurol 35 DisordDrug Targets 7 (2), p. 144-58 (2008)). Several clinically useful analgésies hâve been identified

Page 1 of100

LZ as inhibitors of Na_v charnels. The local anesthetic drugs such as lidocaine block pain by inhibiting Nay channels, and other compounds, such as carbamazepine, lamotrigine, and tricyclic antidepressants that hâve proven effective at reducing pain hâve also been suggested to act by sodium channel inhibition (Soderpalm, B., Anticonvulsants: aspects of their mechanisms of action. EurJPain 6 Suppl A, p. 3-9 (2002); Wang, G. K., Mitchell, J., and Wang, S. Y., Block of persistent late Na⁺ currents by antidepressant sertraline and paroxetine. JMembr Biol 222 (2), p. 79-90 (2008)).

The Nay’s form a subfamily of the voltage-gated ion channel super-family and comprises 9 isoforms, designated Na_vl .1 - Na_vl .9. The tissue localizations of the nine isoforms vary greatly. Na_vl .4 is the primary sodium channel of skeletal muscle, and Nayl .5 is primary sodium channel of cardiac myocytes.

Nay’s 1.7, 1.8 and 1.9 are primarily localized to the peripheral nervous system, while Na_v’s 1.1,1.2, 1.3, and 1.6 are neuronal channels found in both the central and peripheral nervous Systems. The functional behaviors of the nine isoforms are similar but distinct in the spécifies of their voltage-dependent and kinetic behavior (Catterall, W. A., Goldin, A. L., and Waxman, S. G., International Union of Pharmacology. XLVII. Nomenclature and structure-function relationships of voltage-gated sodium channels. Pharmacol Rev 57 (4), p. 397 (2005)).

Immediately upon their discovery, Na_vl .8 channels were identified as likely targets for analgesia (Akopian, A.N., L. Sivilotti, and J.N. Wood, A tetrodotoxin-resistant voltage-gated sodium channel expressedby sensory neurons. Nature, 1996. 379(6562): p. 257-62). Since then, Nayl.8 has been shown to be the most significant carrier of the sodium current that maintains action potential fïring in small

DRG neurons (Blair, N.T. and B.P. Bean, Rôles of tetrodotoxin (TTX)-sensitive Na+ current, TTXresistant Na⁺ current, and Ca²⁺ current in the action potentials of nociceptive sensory neurons. J Neurosci., 2002. 22(23): p. 10277-90). Nayl.8 is essential for spontaneous fïring in damagedneurons, like those that drive neuropathie pain (Roza, C., et al., The tetrodotoxin-resistant Na⁺ channel Na_v1.8 is essential for the expression of spontaneous activity in damaged sensory axons of mice. J. Physiol., 2003.

550(Pt 3): p. 921-6; Jarvis, M.F., et al., A-803467, a potent and sélectiveNayl.8 sodium channel blocker, atténuâtes neuropathie and inflammatory pain in the rat. Proc Natl Acad Sci. USA, 2007.104(20): p. 8520-5; Joshi, S.K., et al., Involvement of the TTX-resistant sodium channel Navl .8 in inflammatory and neuropathie,but not post-operative, pain states. Pain, 2006.123(1-2): pp. 75-82; Lai, J., et al., Inhibition of neuropathie painby decreased expression of the tetrodotoxin-resistant sodium channel, Na_v1.8. Pain,

2002. 95(1-2): p. 143-52; Dong, X.W., et al., Small interfering RNA-mediated sélective knockdown of

Na(y)1.8 tetrodotoxin-resistant sodium channel reverses mechanical allodynia in neuropathie rats. Neuroscience, 2007.146(2): p. 812-21; Huang, H.L., et al., Proteomic profïling of neuromas reveals alterations in protein composition and local protein synthesis in hyper-excitable nerves. Mol Pain, 2008. 4: p. 33; Black, J.A., et al., Multiple sodium channel isoforms and mitogen-activated protein kinases are présent in painful human neuromas. Ann Neurol, 2008. 64(6): p. 644-53; Coward, K., et al., Immunolocalization of SNS/PN3 and NaN/SNS2 sodium channels in human pain states. Pain, 2000.

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85(1-2): p. 41-50; Yiangou, Y., et al., SNS/PN3 and SNS2/NaN sodium channel-like immunoreactivity in human adult and neonate injured sensory nerves. FEBSLett, 2000. 467(2-3): p. 249-52; Ruangsri, S., et al., Relationship of axonal voltage-gated sodium channel 1.8 (Na_v1.8) mRNA accumulation to sciatic nerve injury-induced painful neuropathy in rats. J Biol Chem. 286(46): p. 39836-47). The small DRG neurons where Nayl .8 is expressed include the nociceptors critical for pain signaling. Nayl .8 is the primary channel that médiates large amplitude action potentials in small neurons of the dorsal root ganglia (Blair, N.T. and B.P. Bean, Rôles of tetrodotoxin (TTX)-sensitive Na⁺ current, TTX-resistant Na⁺current, and Ca²⁺ current in the action potentials of nociceptive sensory neurons. J Neurosci., 2002. 22(23): p. 10277-90). Navl .8 is necessary for rapid répétitive action potentials in nociceptors, and for spontaneous activity of damaged neurons. (Choi, J.S. and S.G. Waxman, Physiological interactions between Nayl .7 and Nayl .8 sodium channels: a computer simulation study. JNeurophysiol. 106(6): p. 3173-84; Renganathan, M., T.R. Cummins, and S.G. Waxman, Contribution of Na(v)1.8 sodium channels to action potential electrogenesis in DRG neurons. JNeurophysiol., 2001. 86(2): p. 629-40; Roza, C., et al., The tetrodotoxin-resistant Na⁺ channel Nayl.8 is essential for the expression of spontaneous activity in damaged sensory axons of mice. JPhysiol., 2003. 550(Pt 3): p. 921-6). In depolarized or damaged DRG neurons, Nayl .8 appears to be the primary driver of hyper-excitablility (Rush, A.M., et al., A single sodium channel mutation produces hyper- or hypoexcitability in different types of neurons. Proc Natl Acad Sci USA, 2006.103(21): p. 8245-50). In some animal pain models, Nav1.8 mRNA expression levels hâve been shown to increase in the DRG (Sun, W., et al., Reduced conduction failure of the main axon of polymodal nociceptive C-fïbres contributes to painful diabetic neuropathy in rats. Brain. 135(Pt 2): p. 359-75; Strickland, I.T., et al., Changes in the expression of NaV1.7, Na_v1.8 and Na_v1.9 in a distinct population of dorsal root ganglia innervating the rat knee joint in a model of chronic inflammatory joint pain. EurJPain, 2008.12(5): p. 564-72; Qiu, F., et al., Increased expression of tetrodotoxin-resistant sodium channels Nayl .8 and Na_vl .9 within dorsal root ganglia in a rat model of bone cancer pain. Neurosci. Lett. 512(2): p. 61-6).

The primary drawback to the known Na_v inhibitors is their poor therapeutic window, and this is likely a conséquence of their lack of isoform selectivity. Since Na_vl .8 is primarily restricted to the neurons that sense pain, sélective Nayl .8 blockers are unlikely to induce the adverse events common to non-selective Na_v blockers. Accordingly, there remains a need to develop additional Na_v channel antagonists preferably those that are more Navl .8 sélective and more potent with increased metabolic stability, increased solubility and with fewer side effects.

SUMMARY OF THE INVENTION

It has now been found that compounds of this invention, and pharmaceutically acceptable salts and compositions thereof, are useful as inhibitors of voltage-gated sodium channels. These compounds hâve the general formula I:

Page 3of100

wherein, independently for each occurrence:

R² and R³ are independently hydrogen, halogen, or Cj-Cê alkyl wherein said Ci-Cô alkyl is substituted with 0-6 halogen;

R⁵ is hydrogen, halogen, OH, or Q-Cô alkyl wherein said Ci-C₆ alkyl is substituted with 0-6 halogen and wherein up to two non-adjacent CH₂ units of said Ci-C₆ alkyl may be replaced with -O-;

R⁷ is hydrogen, halogen, or Ci-C₆ alkyl wherein said Ci-C₆ alkyl is substituted with 0-6 halogen and wherein up to two non-adjacent CH₂ units of said Ci-C₆ alkyl may be replaced with -O-; and

X is -PO(OH)₂, -PO(OH)OM⁺, -ΡΟ(Ο')2·2Μ⁺, or -PO(O')2*D²⁺; M⁺ is a pharmaceutically acceptable monovalent cation; and D²⁺ is a pharmaceutically acceptable divalent cation;

provided that R², R³, R⁵, and R⁷ are not simultaneously hydrogen.

The présent invention also relates to novel, solid forms of (4-(2-(4-fluoro-2-methylphenoxy)-4(trifluoromethyl)benzamido)-2-oxopyridin-l(2?/)-yl)methyl dihydrogen phosphate (‘compound 9”). In one embodiment, the présent invention provides solid Form B of compound 9, which is characterized by an X-ray powder diffraction (XRPD) comprising at least three approximate peak positions (degrees 2 thêta [2 θ] + 0.2) when measured using Cu K_a radiation, selected from the group consisting of4.4, 12.7, 13.3, 14.7, 15.2, 16.4, 18.0, 19.1, 19.3, 19.9, 20.2, 20.5, 21.0, 22.2, 23.5 24.2, 24.8, 26.3, 29.6, 30.1 and 31.3, when the XPRD is collected from about 4 to about 40 degrees two thêta (2 0). Solid Form B may also be characterized by an X-ray powder diffraction pattern, as measured using Cu K„ radiation, substantially similar to Figure 2 and an endothermie peak having an onset température at about 210°C as measured by differential scanning calorimetry in which the température is scanned at about 10°C per minute. The présent application also provides a method for preparing crystal Form B of compound 9 by suspending a solid material of free compound 9 in a solvent System comprising water, an organic solvent or an organic solvent/water mixture and isolating the solid. In another embodiment, the présent invention provides amorphous Form C of compound 9, which is characterized by an X-ray powder diffraction (XRPD) substantially similar to that in Figure 5. In another embodiment, the présent invention provides a srpay diy dispersion of amorphous Form C of compound 9, which is characterized by an X-ray powder diffraction (XRPD) Page 4 of 100 substantially similar to that in Figure 6.

In addition to the compounds provided herein, the présent invention further provides pharmaceutically acceptable compositions comprising a compound of formula I and a pharmaceutically acceptable carrier, adjuvant, or vehicle.

In another embodiment, the présent invention relates to a pharmaceutical composition comprising a compound of formula I; a pharmaceutically acceptable carrier, adjuvant, or vehicle; and an additional therapeutic agent.

In another embodiment, the présent invention relates to a method of treating or lessening the severity of a variety of diseases, disorders, or conditions in a subject, including, but not limited to, chronic pain, gut pain, neuropathie pain, musculoskeletal pain, acute pain, inflammatory pain, cancer pain, idiopathic pain, multiple sclerosis, Charcot-Marie-Tooth syndrome, incontinence, pathological cough, or cardiac arrhythmia comprising administering to the subject a therapeutically effective amount of a compound of formula I.

In another embodiment, the présent invention relates to a method of treating or lessening the severity of a variety of diseases, disorders, or conditions in a subject, including, but not limited to, chronic pain, gut pain, neuropathie pain, musculoskeletal pain, acute pain, inflammatory pain, cancer pain, idiopathic pain, multiple sclerosis, Charcot-Marie-Tooth syndrome, incontinence, pathological cough, or cardiac arrhythmia comprising administering to the subject a therapeutically effective amount of a compound of formula I and an additional therapeutic agent.

BRIEF DESCRIPTION OF THE DRAWINGS

Figure 1 is a thermal ellipsoid plot of one symmetry independent molécule of crystalline Form B of (4-(2-(4-fluoro-2-methylphenoxy)-4-(trifluoromethyl)benzamido)-2-oxopyridin-l(2H)-yl)methyl dihydrogen phosphate.

Figure 2 shows an X-ray powder diffraction pattern of solid Form B of (4-(2-(4-fluoro-2methylphenoxy)-4-(trifluoromethyl)benzamido)-2-oxopyridin-1 (227)-yl)methyl dihydrogen phosphate.

Figure 3 shows a DSC (DifferentialScanning Calorimetry) thermogram of solid Form B of (4-(2-(4fhioro-2-methylphenoxy)-4-(trifhioromethyl)benzamido)-2-oxopyridin-l(27ï)-yl)methyl dihydrogen phosphate.

Figure 4 shows a TGA (thermal gravimétrie analysis) thermogram of solid Form B of (4-(2-(4-fluoro2-methylphenoxy)-4-(trifluoromethyl)benzamido)-2-oxopyridin-l(2H)-yl)methyl dihydrogen phosphate.

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Figure 5 shows an X-ray powder diffraction pattern of solid neat amorphous Form C of (4-(2-(4fluoro-2-methylphenoxy)-4-(trifhiorornethyl)benzamido)-2-oxopyridin-l(277)-yl)methyl dihydrogen phosphate.

Figure 6 shows an X-ray powder diffraction pattern of amorphous Form C from a spray dry dispersion of (4-(2-(4-fluoro-2-methylphenoxy)-4-(trifluoromethyl)benzamido)-2-oxopyridin-l (277)yl)methyl dihydrogen phosphate.

DETAILED DESCRIPTION OF THE INVENTION

The instant compounds of formula I are prodrugs of their respective parent compounds. Thus, the activity exhibited upon administration of the prodrug is principally due to the presence of the parent compound that results from cleavage of the prodrug.

The présent invention also relates to novel, solid forms of (4-(2-(4-fluoro-2-methylphenoxy)-4(trifluoromethyl)benzamido)-2-oxopyridin-l(2Z7)-yl)methyl dihydrogen phosphate (“compound 9”). In one embodiment, the présent invention provides a free crystalline form of compound 9 (Form B). In another embodiment, the présent invention provides a process for preparing solid Form B of compound 9. In another embodiment, the présent invention provides an amorphous form of compound 9 (Form C). In yet another embodiment, the présent invention provides a method of preparing amorphous Form C of compound 9.

The term “prodrug” refers to compounds which are drug precursors which, following administration and absorption, release the drug in vivo via some metabolic process. In general, a prodrug possesses less biological activity than its parent drug. A prodrug may also improve the physical properties of the parent drug and/or it may also improve overall drug efficacy, for example through the réduction of toxicity and unwanted effects of a drug by controlling its absorption, blood levels, metabolic distribution and cellular uptake.

The term “parent compound” or “parent drug” refers to the biologically active entity that is released via enzymatic action of a metabolic or a catabolic process, or via a chemical process following administration of the prodrug. The parent compound may also be the starting material for the préparation of its corresponding prodrug.

The monovalent cations defined by M⁺ include ammonium (e.g., N(R⁹)4, wherein R⁹ is H or C1-C4 alkyl), alkali métal ions such as sodium, lithium and potassium ions, dicyclohexylamine ion, and Nmethyl-D-glucamine ion. The divalent cations defined by D²⁺ include- alkaline earth métal ions such as calcium and magnésium ions, as well as divalent aluminum ions. Also included are amino acid cations such as monovalent or divalent ions of arginine, lysine, omithine, and so forth. If M⁺ is a monovalent cation, it is recognized that if the définition 2M⁺ is présent, each of M⁺ may be the same or different. In addition, it is similarly recognized that if the définition 2M⁺ is présent, a divalent cation D²⁺ may instead be présent. Also, the basic nitrogen-containing groups may be quatemized

Page 6 of100 with such agents as: lower alkyl halides, such as methyl, ethyl, propyl, and butyl chloride, bromides and iodides; dialkyl sulfates like dimethyl, diethyl, dibutyl; diamyl sulfates; long chain halides such as decyl, lauryl, myristyl and stearyl chlorides, bromides and iodides; aralkyl halides like benzyl bromide and others.

The prodrugs and solid forms of the présent invention are characterized by unexpectedly high aqueous solubility. This solubility facilitâtes administration of higher doses of the prodrug, resulting in a greater drug load per unit dosage.

For purposes of this invention, the chemical éléments are identified in accordance with the Periodic Table of the Eléments, CAS version, Handbook of Chemistry and Physics, 75* Ed. Additionally, general principles of organic chemistry are described in “Organic Chemistry,” Thomas Sorrell, University Science Books, Sausalito: 1999, and “March’s Advanced Organic Chemistry,” 5^th Ed., Ed.: Smith, M.B. and March, J., John Wiley & Sons, New York: 2001, the entire contents of which are hereby incorporated by reference.

As one of ordinary skill in the art will recognize, combinations of substituents envisioned by this invention are those combinations that resuit in the formation of stable or chemically feasible compounds. The term “stable,” as used herein, refers to compounds that are not substantially altered when subjected to conditions to allow for their production, détection, and preferably their recovery, purification, and use for one or more of the purposes disclosed herein. In some embodiments, a stable compound or chemically feasible compound is one that is not substantially altered when kept at a température of 40°C or less, in the absence of moisture or other chemically reactive conditions, for at least a week.

The term “alkyl” as used herein, means a straight-chain (i.e., unbranched) or branched, substituted or unsubstituted hydrocarbon chain that is completely saturated or that contains one or more units of unsaturation. Suitable alkyl groups include, but are not limited to, linear or branched, and substituted or unsubstituted alkyl.

The term “halogen” or “halo” as used herein, means F, Cl, Br or I.

The phrase “up to,” as used herein, refers to zéro or any integer number that is equal or less than the number following the phrase. For example, “up to 4” means any one of 0, 1,2, 3, and 4.

Within a définition of a term as, for example, R⁷ when a CH₂ unit or, interchangeably, methylene unit may be replaced by -O-, it is meant to include any CH₂ unit, including a CH₂ within a terminal methyl group. For example, CH₂CH₂CH₂OH is within the définition of Ci-C₆ alkyl wherein up to two non-adjacent CH₂ units may be replaced by -O- because the CH₂ unit of the terminal methyl group has been replaced by -O-.

Unless otherwise stated, structures depicted herein are also meant to include ail isomeric (e.g.,

Page 7 of100 enantiomeric, diastereomeric, and géométrie (or conformational)) forms of the structure; for example, the R and S configurations for each asymmetric center, (Z) and (E) double bond isomers, and (Z) and (E) conformational isomers. Therefore, single stereochemical isomers as well as enantiomeric, diastereomeric, and géométrie (or conformational) mixtures of the présent compounds are within the scope of the invention. Unless otherwise stated, ail tautomeric forms of the compounds of the invention are within the scope of the invention. Thus, included within the scope of the invention are tautomers of compounds of formula I. The structures also include zwitterioinc forms of the compounds or salts of fomula where appropriate.

Additionally, unless otherwise stated, structures depicted herein are also meant to include compounds that differ only in the presence of one or more isotopically enriched or isotopicallylabeled atoms. The isotopically-labeled compounds may hâve one or more atoms replaced by an atom having an atomic mass or mass number usually found in nature. Examples of isotopes présent in compounds of formula I include isotopes of hydrogen, carbon, nitrogen, oxygen, phosphorus, fluorine and chlorine, such as, but not limited to, ²H, ³H, ¹³C, ¹⁴C, ¹⁵N, ¹⁸0,¹⁷0,³⁵S and ¹⁸F. Certain isotopically-labeled compounds of formula I, in addition to being useful as as therapuetic agents, are also useful in drug and/or substrate tissue distribution assays, as analytical tools or as probes in other biological assays. In one aspect of the présent invention, tritiated (e.g., ³H) and carbon-14 (e.g., ¹⁴C) isotopes are useful given their ease of detectability. In another aspect of the présent invention, replacement of one or more hydrogen atoms with heavier isotopes such as deuterium, (e.g., ²H) can afford certain therapeutic advantages.

Tn one embodiment, the invention features a compound of formula I and the attendant définitions, wherein R² is H. In another embodiment, R² is halogen. In another embodiment, R² is Cl. In another embodiment, R² is F. In another embodiment, R² is Ci-C6 alkyl wherein said Ci-Cg alkyl is substituted with 0-6 halogen. In another embodiment, R² is CF3. In another embodiment, R² is H, Cl orCF₃.

Tn another embodiment, the invention features a compound of formula I and the attendant définitions, wherein R³ is H. Tn another embodiment, R³ is halogen. In another embodiment, R³ is Cl. In another embodiment, R³ is Ci-C6 alkyl wherein said Ci-C6 alkyl is substituted with 0-6 halogen. In another embodiment, R³ is CF3. In another embodiment, R³ is CF₂CF₃.

Tn another embodiment, the invention features a compound of formula I and the attendant définitions, wherein R⁵ is H. Tn another embodiment, R⁵ is halogen. In another embodiment, R⁵ is Cl. In another embodiment, R⁵ is F. Tn another embodiment, R⁵ is Ci-C6 alkyl. In another embodiment, R⁵ is CH3. In another embodiment, R⁵ is Ci-C6 alkyl wherein said Cj-C6 alkyl is substituted with 0-6 halogen wherein one CH2 unit of said Ci-C6 alkyl is replaced with -O-. In another embodiment, R⁵ is OCH3. Tn another embodiment, R⁵ is OH. In another embodiment, R⁵ is OCF₃.

Page 8 of 100

In another embodiment, the invention features a compound of formula I and the attendant définitions, wherein R⁷ is H. In another embodiment, R⁷ is halogen. In another embodiment, R⁷ is F. In another embodiment, R⁷ is Ci-C₆ alkyl wherein said Ci-C₆ alkyl is substituted with 0-6 halogen wherein two non-adjacent CH2 units of said Ci-C₆ alkyl are replaced with -O-. In another embodiment, R⁷ is

OCH3. In another embodiment, R⁷ is OCF₃.

In another embodiment, the invention features a compound of formula I and the attendant définitions, wherein X is -PO(OH)O’M⁺, -PO(O‘)2*2M⁺; or -PO(O')2«D²⁺; M⁺ is Li⁺, Na⁺, K⁺ or N(R⁹)₄ ⁺; wherein each R⁹ is independently H or a C1-C4 alkyl group and D²⁺ is Mg²⁺, Ca²⁺ or Ba²⁺.

In another embodiment, the invention features a compound of formula I and the attendant définitions, wherein X is -PO(OH)O'M⁺ and M⁺ is Li⁺. In one embodiment, X is -PO(OH)O'M⁺ and M⁺ is Na⁺. In another embodiment, X is -PO(OFI)O'M⁺ and M⁺ is K⁺. In another embodiment, X is -PO(OH)O’ M⁺ and M⁺ is N(R⁹)4⁺; wherein each R⁹ is independently H or a Ci-C4 alkyl group. In another embodiment, X is -PO(OH)O'M⁺ and M⁺ is N(R⁹)4⁺; wherein each R⁹ is a CH3 group. In another embodiment, the invention features a compound of formula I and the attendant définitions, wherein X is -ΡΟ(Ο')₂·2Μ⁺ and M⁺ is Li⁺. In one embodiment, X is -ΡΟ(Ο’)2·2Μ⁺ and M⁺ is Na⁺. In another embodiment, X is -ΡΟ(Ο')2·2Μ⁺ and M⁺ is K⁺. In another embodiment, X is -ΡΟ(Ο')2·2Μ⁺ and M⁺is N(R⁹)4⁺; wherein each R⁹ is independently H or a Cj-C4 alkyl group. In another embodiment, X is -ΡΟ(Ο)2·2Μ⁺ and M⁺ is N(R⁹)4⁺; wherein each R⁹ is a CH3 group.

In another embodiment, the invention features a compound of formula I and the attendant définitions, wherein X is -PO(O')₂*D²⁺ and D²⁺ is Mg²⁺, Ca²⁺ or Ba²⁺. In one embodiment, X is -PO(O‘)2*D²⁺and D²⁺ is Mg²⁺. In another embodiment, X is -PO(O)2’D²⁺ and D²⁺ is Ca²⁺. In another embodiment, X is -PO(O')₂*D²⁺ and D²⁺ is Ba²⁺.

In another embodiment, the invention features a compound of formula I and the attendant définitions, wherein X is -PO(OH)₂.

In another embodiment, the invention features a compound of formula I and the attendant définitions, wherein R² is Cl, R³ is Cl, R⁵ is OCH3, R⁷ is F and X is -PO(OH)2.

In another embodiment, the invention features a compound of formula I and the attendant définitions, wherein R² is Cl, R³ is Cl, R⁵ is OCH3, R⁷ is F and X is -ΡΟ(Ο')2·2Μ⁺, M⁺ is Li⁺, Na⁺, K⁺ or N(R⁹)4⁺wherein each R⁹ is independently H or a Ci-C4 alkyl group.

In another embodiment, the invention features a compound of formula I and the attendant définitions, wherein R² is Cl, R³ is Cl, R⁵ is OCH₃, R⁷ is F and X is-PO(OH)O’M⁺, M⁺ is Li⁺, Na⁺, K⁺ or N(R⁹)4⁺wherein each R⁹ is independently H or a Ci-C4 alkyl group.

Tn another embodiment, the invention features a compound of formula I and the attendant définitions, wherein R³ is CF2CF3, R⁵ is OCH3, R⁷ is F and X is -PO(OH)₂.

In another embodiment, the invention features a compound of formula I and the attendant définitions, Page 9 of100 wherein R³ is CF2CF3, R⁵ is OCH3, R⁷ is F and X is -ΡΟ(Ο’)2·2Μ⁺, M⁺ is Li⁺, Na⁺, K⁺ or N(R⁹)4⁺wherein each R⁹ is independently H or a C]-C₄ alkyl group.

In another embodiment, the invention features a compound of formula I and the attendant définitions, wherein R³ is CF2CF3, R⁵ is OCH3, R⁷ is F and X is-PO(OH)O‘M⁺, M⁺ is Li⁺, Na⁺, K⁺ or N(R⁹)4⁺wherein each R⁹ is independently H or a C]-C4 alkyl group.

In another embodiment, the invention features a compound of formula I and the attendant définitions, wherein R³ is CF3, R⁵ is CH3, R⁷ is F and X is -PO(OH)₂.

In another embodiment, the invention features a compound of formula I and the attendant définitions, wherein R³ is CF3, R⁵ is CH3, R⁷ is F, X is -ΡΟ(Ο')₂·2Μ⁺, M⁺ is Li⁺, Na⁺, K⁺. In another embodiment, M⁺ is Li⁺. In yet another embodiment, M⁺ is Na⁺.

In another embodiment, the invention features a compound of formula I and the attendant définitions, wherein R³ is CF3, R⁵ is CH3, R⁷ is F, X is -PO(O’)2-D²⁺ and D²⁺ is Mg²⁺, Ca²⁺ or Ba²⁺. In one embodiment, X is -PO(O')2’D²⁺ and D²⁺ is Mg²⁺. In another embodiment, X is -PO(O)₂*D²⁺ and D²⁺is Ca²⁺.

In another embodiment, the invention features a compound of formula I and the attendant définitions, wherein R² is CF3, R⁵ is CH3, R⁷ is F and X is -PO(OH)₂.

In another embodiment, the invention features a compound of formula I and the attendant définitions, wherein R² is CF3, R⁵ is CH3, R⁷ is F, X is -ΡΟ(Ο’)₂·2Μ⁺, M⁺ is Li⁺, Na⁺, K⁺. In another embodiment, M⁺ is Li⁺. In yet another embodiment, M⁺ is Na⁺.

In another embodiment, the invention features a compound of formula I and the attendant définitions, wherein R² is CF3, R⁵ is CH3, R⁷ is F, X is -PO(O’)2*D²⁺ and D²⁺ is Mg²⁺, Ca²⁺ or Ba²⁺. In one embodiment, X is -PO(O')2’D²⁺ and D²⁺ is Mg²⁺. In another embodiment, X is -PO(O')₂*D²⁺ and D²⁺is Ca²⁺.

In another embodiment, the invention features a compound of formula I and the attendant définitions, wherein R³ is Cl, R⁵ is CH3, R⁷ is F and X is -PO(OH)2.

In another embodiment, the invention features a compound of formula I and the attendant définitions, wherein R³ is Cl, R⁵ is CH3, R⁷ is F and X is -PO(O‘)2*2M⁺, M⁺ is Li⁺, Na⁺, K⁺ or N(R⁹)4⁺ wherein each R⁹ is independently H or a CrC₄ alkyl group.

In another embodiment, the invention features a compound of formula I and the attendant définitions, wherein R³ is Cl, R⁵ is CH3, R⁷ is F and X is-PO(OH)O’M⁺, M⁺ is Li⁺, Na⁺, K⁺ or N(R⁹)4⁺ wherein each R⁹ is independently H or a Ci-C₄ alkyl group.

Page 10 of 100

In another embodiment, the invention features a compound of formula I and the attendant définitions, wherein R² is CF₃, R⁷ is F, X is -PO(O')2*2M⁺, M⁺ is Li⁺, Na⁺, K⁺. In another embodiment, M⁺ is

Li⁺. In yet another embodiment, M⁺ is Na⁺.

In another embodiment, the invention features a compound of formula I and the attendant définitions, wherein R² is CF₃, R⁷ is F, X is -PO(O’)2*D²⁺ and D²⁺ is Mg²⁺, Ca²⁺ or Ba²⁺. In one embodiment, X is -PO(O‘)2*D²⁺ and D²⁺ is Mg²⁺. In another embodiment, X is -PO(O’)2’D²⁺ and D²⁺ is Ca²⁺.

In another embodiment, the invention provides a compound of formula I-A

wherein, independently for each occurrence:

R² is halogen, or Ci-Cg alkyl wherein said Ci-Cg alkyl is substituted with 0-6 halogen;

R⁵ is halogen, OH, or Ci-Cô alkyl wherein said Ci-Cg alkyl is substituted with 0-6 halogen and wherein up to two non-adjacent CH₂ units of said Cj-Cô alkyl may be replaced with -O-;

R⁷ is halogen, or C]-C₆ alkyl wherein said Ci-C₆ alkyl is substituted with 0-6 halogen and wherein up to two non-adjacent 0¾ units of said Cj-Cg alkyl may be replaced with -O-; and

X is -PO(OH)₂, -PO(OH)O'M⁺, -PO(O')2’2M⁺, or -PO(O')2’D²⁺; M⁺ is a pharmaceutically acceptable monovalent cation; and D²⁺ is a pharmaceutically acceptable divalent cation.

In another embodiment, the invention features a compound of formula I-A and the attendant définitions, wherein R² is halogen. In another embodiment, R² is Cl. In another embodiment, R² is F. In another embodiment, R² is Ci-C6 alkyl wherein said Ci-Cg alkyl is substituted with 0-6 halogen. In another embodiment, R² is CF3. In another embodiment, R² is Cl or CF₃.

In another embodiment, the invention features a compound of formula I-A and the attendant définitions, wherein R⁵ is halogen. In another embodiment, R⁵ is Cl. In another embodiment, R⁵ is F. In another embodiment, R⁵ is Ci-C6 alkyl. In another embodiment, R⁵ is CH3. In another embodiment, R⁵ is Ci-C6 alkyl wherein said CrCg alkyl is substituted with 0-6 halogen wherein one CH2 unit of said C]-Cg alkyl is replaced with-O-. In another embodiment, R⁵ is OCH3. In another embodiment, R⁵ is OH. In another embodiment, R⁵ is OCF3. In another embodiment, R⁵ is F, Cl, CH3, OCH₃, OH or OCF₃.

In another embodiment, the invention features a compound of formula I-A and the attendant définitions,

Page 11 of 100 wherein R⁷ is halogen. In another embodiment, R⁷ is F. In another embodiment, R⁷ is Ci-C6 alkyl wherein said Ci-Cé alkyl is substituted with 0-6 halogen wherein two non-adjacent CH2 units of said CiC6 alkyl are replaced with -O-. In another embodiment, R⁷ is OCH3. In another embodiment, R⁷ is OCF3. In another embodiment, R⁷ is F, OCH3 or OCF₃.

In another embodiment, the invention features a compound of formula I-A and the attendant définitions, wherein X is -PO(OH)₂, -PO(OH)O’M⁺; -ΡΟ(Ο’)2·2Μ⁺ or -PO(O’)2«D²⁺; wherein M⁺ is Li⁺, Na⁺ or K⁺and wherein D²⁺ is Mg²⁺ or Ca²⁺.

In another embodiment, the invention features a compound of formula I-A and the attendant définitions, wherein R² is CF3, R⁵ is Cl, OCH3 or CH₃ and R⁷ is F. In one embodiment, R² is CF3, R⁵ is Cl and R⁷ is F. In another embodiment, R² is CF3, R⁵ is OCH₃ and R⁷ is F. In another embodiment, R² is CF3, R⁵ is CH3andR⁷is F.

In another embodiment, the invention features a compound of formula I-A and the attendant définitions, wherein R² is CF3, R⁵ is Cl, OCH3 or CH₃, R⁷ is F and X is -PO(OH)2. In one embodiment, R² is CF3, R⁵is Cl, R⁷ is F and X is -PO(OH)2. In another embodiment, R² is CF3, R⁵ is OCH3, R⁷ is F and X is — PO(OH)2. In another embodiment, R² is CF3, R⁵ is CH3, R⁷ is F and X is -PO(OH)₂.

In another embodiment, the invention features a compound of formula I-A and the attendant définitions, wherein X is -PO(OH)O'M⁺, -ΡΟ(Ο’)2·2Μ⁺; or-PO(O‘)2«D²⁺; M⁺ is Li⁺, Na⁺, K⁺ orN(R⁹)4⁺; wherein each R⁹ is independently H or a Ci-C4 alkyl group and D²⁺ is Mg²⁺, Ca²⁺ or Ba²⁺.

In another embodiment, the invention features a compound of formula I-A and the attendant définitions, wherein X is -PO(OH)OM⁺ and M* is Li⁺. In one embodiment, X is -PO(OH)O’M⁺ and M⁺ is Na⁺. In another embodiment, X is -PO(OH)O’M⁺ and M⁺ is K⁺. In another embodiment, X is -PO(OH)O‘M⁺ and M⁺ is N(R⁹)4⁺; wherein each R⁹ is independently H or a Ci-C4 alkyl group. In another embodiment, X is -PO(OH)O‘M⁺ and M⁺ is N(R⁹)4⁺; wherein each R⁹ is a CH3 group.

In another embodiment, the invention features a compound of formula I-A and the attendant définitions, wherein X is -ΡΟ(Ο’)₂·2Μ⁺ and M⁺ is Li⁺. In one embodiment, X is -ΡΟ(Ο')2·2Μ⁺ and M⁺ is Na⁺. In another embodiment, X is -ΡΟ(Ο')2·2Μ⁺ and M⁺ is K⁺. In another embodiment, X is -ΡΟ(Ο’)2·2Μ⁺ and M⁺ is N(R⁹)4⁺; wherein each R⁹ is independently H or a Ci-C₄ alkyl group. In another embodiment, X is -PO(O‘)₂*2M⁺ and M⁺ is N(R⁹)4⁺; wherein each R⁹ is a CH3 group.

In another embodiment, the invention features a compound of formula I-A and the attendant définitions, wherein X is -PO(O’)₂«D²⁺ and D²⁺ is Mg²⁺, Ca²⁺ or Ba²⁺. In one embodiment, X is -PO(O‘)2*D²⁺ and D²⁺ is Mg²⁺. In another embodiment, X is -PO(O’)2*D²⁺ and D²⁺ is Ca²⁺. In another embodiment, X is PO(O’)₂*D²⁺ and D²⁺ is Ba²⁺.

In another embodiment, the invention features a compound of formula I-A and the attendant définitions, wherein X is -PO(OH)₂.

In another embodiment, the invention features a compound of formula I-A and the attendant définitions, wherein R² is CF3, R⁵ is CH3, R⁷ is F and X is -PO(OH)₂.

Page 12 of 100

In another embodiment, the invention features a compound of formula I-A and the attendant définitions, wherein R² is CF3, R⁵ is CH3, R⁷ is F, X is -ΡΟ(Ο’)₂·2Μ⁺, M⁺ is Li⁺, Na⁺, K⁺. In another embodiment,

M⁺ is Li⁺. In yet another embodiment, M⁺ is Na⁺.

In another embodiment, the invention features a compound of formula I-A and the attendant définitions, wherein R² is CF3, R⁵ is CH3, R⁷ is F, X is -PO(O')2-D²⁺ and D²⁺ is Mg²⁺, Ca²⁺ or Ba²⁺. In one embodiment, X is -PO(O')2*D²⁺ and D²⁺ is Mg²⁺. In another embodiment, X is -PO(O')₂*D²⁺ and D²⁺ is Ca²⁺.

In another embodiment, the invention provides a compound of formula I-B

wherein, independently for each occurrence:

R³ is halogen, or Cj-C₆ alkyl wherein said C_rC₆ alkyl is substituted with 0-6 halogen;

R⁵ is halogen, OH, or Ci-Ce alkyl wherein said C|-C₆ alkyl is substituted with 0-6 halogen and wherein up to two non-adjacent CH₂ units of said C|-C₆ alkyl may be replaced with -O-;

R⁷ is halogen, or Ci-C₆ alkyl wherein said Ci-C₆ alkyl is substituted with 0-6 halogen and wherein up to two non-adjacent CH₂ units of said Ci-C₆ alkyl may be replaced with -O-; and

X is -PO(OH)₂, -PO(OH)O’M⁺, -ΡΟ(Ο’)2·2Μ⁺, or -PO(O‘)2*D²⁺; M⁺ is a pharmaceutically acceptable monovalent cation; and D²⁺ is a pharmaceutically acceptable divalent cation.

In another embodiment, the invention features a compound of formula I-B and the attendant définitions, wherein R³ is halogen. In another embodiment, R³ is Cl. In another embodiment, R³ is Ci-Cg alkyl wherein said Cj-Cg alkyl is substituted with 0-6 halogen. In another embodiment, R³ is CF3. In another embodiment, R³ is CF2CF3.

In another embodiment, the invention features a compound of formula I-B and the attendant définitions, wherein R⁵ is halogen. In another embodiment, R⁵ is Cl. In another embodiment, R⁵ is F. In another embodiment, R⁵ is Ci-C6 alkyl. In another embodiment, R⁵ is CH3. In another embodiment, R⁵ is Ci-C₆alkyl wherein said Ci-C₆ alkyl is substituted with 0-6 halogen wherein one CH₂ unit of said Ci-C₆ alkyl is replaced with -O-. In another embodiment, R⁵ is OCH3. In another embodiment, R⁵ is OH. In another embodiment, R⁵ is OCF3.

Page 13 oflOO

In another embodiment, the invention features a compound of formula I-B and the attendant définitions, wherein R⁷ is halogen. In another embodiment, R⁷ is F. In another embodiment, R⁷ is Ci-C6 alkyl wherein said CrC6 alkyl is substituted with 0-6 halogen wherein two non-adjacent CH2 units of said Cr C& alkyl are replaced with -O-. In another embodiment, R⁷ is OCH3. In another embodiment, R⁷ is OCF₃.

In another embodiment, the invention features a compound of formula I-B and the attendant définitions, wherein X is X is -PO(OH)₂, -PO(OH)O’M⁺; -ΡΟ(Ο')2·2Μ⁺ or -PO(O’)2-D²⁺; wherein M⁺ is Li⁺, Na⁺or K⁺ and wherein D²⁺ is Mg²⁺ or Ca²⁺.

In another embodiment, the invention features a compound of formula I-B and the attendant définitions, wherein R³ is CF3, R⁵ is F or CH3 and R⁷ is F. In one embodiment, R³ is Cl, R⁵ is CH₃ and R⁷ is F. In another embodiment, R³ is CF2CF3, R⁵ is OCH3 and R⁷ is F.

In another embodiment, the invention features a compound of formula I-B and the attendant définitions, wherein R³ is CF3, R⁵ is F or CH3, R⁷ is F and X is -PO(OH)₂. In one embodiment, R³ is Cl, R⁵ is CH3, R⁷ is F and X is -PO(OH)2. In another embodiment, R³ is CF2CF3, R⁵ is OCH3, R⁷ is F and X is PO(OH)₂.

In another embodiment, the invention features a compound of formula I-B and the attendant définitions, wherein X is -PO(OH)O'M⁺, -ΡΟ(Ο')2·2Μ⁺; or -PO(O’)2«D²⁺; M⁺ is Li⁺, Na⁺, K⁺ or N(R⁹)₄ ⁺; wherein each R⁹ is independently H or a C1-C4 alkyl group and D²⁺ is Mg²⁺, Ca²⁺ or Ba²⁺.

In another embodiment, the invention features a compound of formula I-B and the attendant définitions, wherein X is -PO(OH)O'M⁺ and M⁺ is Li⁺. In one embodiment, X is -PO(OH)O’M⁺ and M* is Na⁺. In another embodiment, X is -PO(OH)O'M⁺ and M⁺ is K⁺. In another embodiment, X is -PO(OH)O’M⁺ and M⁺ is N(R⁹)4⁺; wherein each R⁹ is independently H or a Ci-C4 alkyl group. In another embodiment, X is -PO(OH)O'M⁺ and M⁺ is N(R⁹)4⁺; wherein each R⁹ is a CH3 group.

In another embodiment, the invention features a compound of formula I-B and the attendant définitions, wherein X is -ΡΟ(Ο’)₂·2Μ⁺ and M⁺ is Li⁺. In one embodiment, X is -ΡΟ(Ο’)2·2Μ⁺ and M⁺ is Na⁺. In another embodiment, X is -ΡΟ(Ο’)2·2Μ⁺ and M⁺ is K⁺. In another embodiment, X is -ΡΟ(Ο’)2·2Μ⁺ and M⁺ is N(R⁹)4⁺; wherein each R⁹ is independently H or a Ci-C₄ alkyl group. In another embodiment, X is -PO(O‘)₂*2M⁺ and M⁺ is N(R⁹)4⁺; wherein each R⁹ is a CH3 group.

Tn another embodiment, the invention features a compound of formula I-B and the attendant définitions, wherein X is -PO(O’)₂*D²⁺ and D²⁺ is Mg²⁺, Ca²⁺ or Ba²⁺. In one embodiment, X is -PO(O‘)2’D²⁺ and D²⁺ is Mg²⁺. In another embodiment, X is -PO(O’)2«D²⁺ and D²⁺ is Ca²⁺. In another embodiment, X is PO(O’)₂*D²⁺ and D²⁺ is Ba²⁺.

In another embodiment, the invention features a compound of formula I-B and the attendant définitions, wherein X is -PO(OH)₂.

Tn another embodiment, the invention features a compound of formula I-B and the attendant définitions, wherein R³ is CF2CF3, R⁵ is OCH3, R⁷ is F and X is -PO(OH)₂.

In another embodiment, the invention features a compound of formula I-B and the attendant définitions,

Page 14 of 100 wherein R³ is CF2CF3, R⁵ is OCH3, R⁷ is F and X is -ΡΟ(Ο’)2·2Μ⁺, M⁺ is Li⁺, Na⁺, K⁺ or N(R⁹)4⁺ wherein each R⁹ is independently H or a Ci-C₄ alkyl group.

In another embodiment, the invention features a compound of formula I-B and the attendant définitions, wherein R³ is CF2CF3, R⁵ is OCH3, R⁷ is F and X is-PO(OH)O’M⁺, M⁺ is Li⁺, Na⁺, K⁺ or N(R⁹)₄ ⁺ wherein each R⁹ is independently H or a C1-C4 alkyl group.

In another embodiment, the invention features a compound of formula I-B and the attendant définitions, wherein R³ is CF3, R⁵ is CH3, R⁷ is F and X is -PO(OH)₂.

In another embodiment, the invention features a compound of formula I-B and the attendant définitions, wherein R³ is CF3, R⁵ is CH3, R⁷ is F, X is -PO(O')2*2M⁺, M⁺ is Li⁺, Na⁺, K⁺. In another embodiment, M* is Li⁺. In yet another embodiment, M⁺ is Na⁺.

In another embodiment, the invention features a compound of formula I-B and the attendant définitions, wherein R³ is CF3, R⁵ is CH3, R⁷ is F, X is -PO(O’)2«D²⁺ and D²⁺ is Mg²⁺, Ca²⁺ or Ba²⁺. In one embodiment, X is -PO(O')2’D²⁺ and D²⁺ is Mg²⁺. In another embodiment, X is -PO(O')₂*D²⁺ and D²⁺ is Ca²⁺.

In another embodiment, the invention features a compound of formula I-B and the attendant définitions, wherein R³ is Cl, R⁵ is CH3, R⁷ is F and X is -PO(OH)2.

In another embodiment, the invention features a compound of formula I-B and the attendant définitions, wherein R³ is Cl, R⁵ is CH3, R⁷ is F and X is -ΡΟ(Ο')2·2Μ⁺, M⁺ is Li⁺, Na⁺, K⁺ or N(R⁹)4⁺ wherein each R⁹ is independently H or a CrC₄ alkyl group.

In another embodiment, the invention features a compound of formula I-B and the attendant définitions, wherein R³ is Cl, R⁵ is CH3, R⁷ is F and X is-PO(OH)O’M⁺, M⁺ is Li⁺, Na⁺, K⁺ or N(R⁹)4⁺ wherein each R⁹ is independently H or a Ci-C₄ alkyl group.

In another embodiment, the invention provides a compound of formula I-C

wherein, independently for each occurrence:

R² is halogen, or Ci-C₆ alkyl wherein said Ci-C₆ alkyl is substituted with 0-6 halogen;

Page 15 of 100

R⁷ is haiogen, or Cj-Cô alkyl wherein said Ci-Ce alkyl is substituted with 0-6 haiogen and wherein up to two non-adjacent CH₂ units of said Ci-C₆ alkyl may be replaced with -O-; and

X is -PO(OH)₂, -PO(OH)O'M⁺, -ΡΟ(Ο')2·2Μ⁺, or -PO(O‘)2*D²⁺; M⁺ is a pharmaceutically acceptable monovalent cation; and D²⁺ is a pharmaceutically acceptable divalent cation.

In another embodiment, the invention features a compound of formula I-C and the attendant définitions, wherein R² is Cj-C₆ alkyl wherein said Cj-Cô alkyl is substituted with 0-6 haiogen. In another embodiment, R² is CF3.

In another embodiment, the invention features a compound of formula I-C and the attendant définitions, wherein R⁷ is haiogen. In one embodiment, R⁷ is F. In another embodiment, R⁷ is Ci-Cô alkyl wherein said Ci-Cg alkyl is substituted with 0-6 haiogen and wherein up to two non-adjacent CH2 units of said Cj-Cë alkyl may be replaced with -O-. In one embodiment, R⁷ is OCF3.

In another embodiment, the invention features a compound of formula I-C and the attendant définitions, wherein X is -PO(OH)₂, -PO(OH)O'M⁺; -ΡΟ(Ο')2·2Μ⁺ or -PO(O’)2*D²⁺; wherein M⁺ is Li⁺, Na⁺ or K⁺ and wherein D²⁺ is Mg²⁺ or Ca²⁺.

In another embodiment, the invention features a compound of formula I-C and the attendant définitions, wherein R² is CF3 and R⁷ is F or OCF3.

In another embodiment, the invention features a compound of formula I-C and the attendant définitions, wherein R² is CF3, R⁷ is F or OCF3 and X is -PO(OH)₂.

In another embodiment, the invention features a compound of formula I-C and the attendant définitions, wherein X is -PO(OH)O'M⁺, -PO(O‘)2*2M⁺; or -PO(O')2*D²⁺; M⁺ is Li⁺, Na⁺, K⁺ or N(R⁹)₄ ⁺; wherein each R⁹ is independently H or a C1-C4 alkyl group and D²⁺ is Mg²⁺, Ca²⁺ or Ba²⁺.

In another embodiment, the invention features a compound of formula I-C and the attendant définitions, wherein X is -PO(OH)O’M⁺ and M⁺ is Li⁺. In one embodiment, X is -PO(OH)O‘M⁺ and M⁺ is Na⁺. In another embodiment, X is -PO(OH)O'M⁺ and M⁺ is K⁺. In another embodiment, X is -PO(OH)OM⁺ and M⁺ is N(R⁹)4⁺; wherein each R⁹ is independently H or a Cj-C4 alkyl group. In another embodiment, X is -PO(OH)O‘M⁺ and M⁺ is N(R⁹)4⁺; wherein each R⁹ is a CH3 group.

In another embodiment, the invention features a compound of formula I-C and the attendant définitions, wherein X is -PO(O‘)₂*2M⁺ and M⁺ is Li⁺. In one embodiment, X is -PO(O‘)2*2M⁺ and M⁺ is Na⁺. In another embodiment, X is -PO(O‘)2*2M⁺ and M⁺ is K⁺. In another embodiment, X is -ΡΟ(Ο’)2·2Μ⁺ and M⁺ is N(R⁹)4⁺; wherein each R⁹ is independently H or a C1-C4 alkyl group. In another embodiment, X is -ΡΟ(Ο’)₂·2Μ⁺ and M⁺ is N(R⁹)4⁺; wherein each R⁹ is a CH3 group.

In another embodiment, the invention features a compound of formula I-C and the attendant définitions, wherein X is -PO(O‘)₂«D²⁺ and D²⁺ is Mg²⁺, Ca²⁺ or Ba²⁺. In one embodiment, X is -PO(O')2*D²⁺ and D²⁺ is Mg²⁺. In another embodiment, X is -PO(O')2«D²⁺ and D²⁺ is Ca²⁺. In another embodiment, X is PO(O’)₂«D²⁺ and D²⁺ is Ba²⁺.

Page 16 of 100

In another embodiment, the invention features a compound of formula I-C and the attendant définitions, wherein X is -PO(OH)₂.

In another embodiment, the invention features a compound of formula I-C and the attendant définitions, wherein R² is CF3, R⁵ is CH3, R⁷ is F and X is -PO(OH)₂.

In another embodiment, the invention features a compound of formula I-C and the attendant définitions, wherein R² is CF3, R⁷ is F, X is -ΡΟ(Ο')2·2Μ⁺, M⁺ is Li⁺, Na⁺, K⁺. In another embodiment, M⁺ is Li⁺. In yet another embodiment, M⁺ is Na⁺.

Jh another embodiment, the invention features a compound of formula I-C and the attendant définitions, wherein R² is CF3, R⁷ is F, X is -PO(O')2*D²⁺ and D²⁺ is Mg²⁺, Ca²⁺ or Ba²⁺. In one embodiment, X is PO(O')2«D²⁺ and D²⁺ is Mg²⁺. In another embodiment, X is -PO(O')2*D²⁺ and D²⁺ is Ca²⁺.

In another embodiment, the invention provides a compound of formula I-D

I-D wherein, independently for each occurrence:

R³ is halogen, or Ci-C₆ alkyl wherein said Ci-Cô alkyl is substituted with 0-6 halogen;

R⁷ is halogen, or Ci-Cô alkyl wherein said Ci-C₆ alkyl is substituted with 0-6 halogen and wherein up to two non-adjacent CH₂ units of said Ci-C₆ alkyl may be replaced with -O-; and

X is -PO(OH)₂, -PO(OH)CTM⁺, -ΡΟ(Ο’)2·2Μ⁺, or -PO(O')2*D²⁺; M⁺ is a pharmaceutically acceptable monovalent cation; and D²⁺ is a pharmaceutically acceptable divalent cation.

In another embodiment, the invention features a compound of formula I-D and the attendant définitions, wherein R³ is Ci-C6 alkyl wherein said Ci-C6 alkyl is substituted with 0-6 halogen. In another embodiment, R³ is CF3. In another embodiment, R³ is CF₂CF₃.

In another embodiment, the invention features a compound of formula I-D and the attendant définitions, wherein R⁷ is halogen. In one embodiment, R⁷ is F. In another embodiment, R⁷ is Ci-C6 alkyl wherein said Ci-C6 alkyl is substituted with 0-6 halogen and wherein up to two non-adjacent CH2 units of said Cr C6 alkyl may be replaced with -O-. In one embodiment, R⁷ is OCF3.

Page 17 oflOO

In another embodiment, the invention features a compound of formula I-D and the attendant définitions, wherein X is -PO(OH)₂, -PO(OH)OM⁺; -ΡΟ(Ο’)2·2Μ⁺ or -PO(O')2-D²⁺; wherein M* is Li⁺, Na⁺ or K⁺and wherein D²⁺ is Mg²⁺ or Ca²⁺.

In another embodiment, the invention features a compound of formula I-D and the attendant définitions, wherein R³ is CF3 and R⁷ is CF3. In another embodiment, R³ is CF₂CF₃ and R⁷ is F.

In another embodiment, the invention features a compound of formula I-D and the attendant définitions, wherein R³ is CF3, R⁷ is CF3 and X is -PO(OH)₂. In another embodiment, R³ is CF2CF3, R⁷ is F and X is -PO(OH)2.

In another embodiment, the invention features a compound of formula I-D and the attendant définitions, wherein X is -PO(OH)O'M⁺, -ΡΟ(Ο’)2·2Μ⁺; or -PO(O’)2«D²⁺; M⁺ is Li⁺, Na⁺, K⁺ or N(R⁹)₄ ⁺; wherein each R⁹ is independently H or a C1-C4 alkyl group and D²⁺ is Mg²⁺, Ca²⁺ or Ba²⁺.

In another embodiment, the invention features a compound of formula I-D and the attendant définitions, wherein X is -PO(OH)O'M⁺ and M⁺ is Li⁺. In one embodiment, X is -PO(OH)O'M⁺ and M* is Na⁺. In another embodiment, X is -PO(OH)O'M⁺ and M⁺ is K⁺. In another embodiment, X is -PO(OH)O‘M⁺ and M⁺ is N(R⁹)4⁺; wherein each R⁹ is independently H or a C1-C4 alkyl group. In another embodiment, X is -PO(OH)O’M⁺ and M⁺ is N(R⁹)4⁺; wherein each R⁹ is a CH3 group.

In another embodiment, the invention features a compound of formula I-D and the attendant définitions, wherein X is -ΡΟ(Ο)₂·2Μ⁺ and M⁺ is Li⁺. In one embodiment, X is -ΡΟ(Ο’)2·2Μ⁺ and M⁺ is Na⁺. In another embodiment, X is -ΡΟ(Ο)2·2Μ⁺ and M⁺ is K⁺. In another embodiment, X is -ΡΟ(Ο’)2·2Μ⁺ and M⁺ is N(R⁹)4⁺; wherein each R⁹ is independently H or a C1-C4 alkyl group. In another embodiment, X is -ΡΟ(Ο)2·2Μ⁺ and M⁺ is N(R⁹)4⁺; wherein each R⁹ is a CH3 group.

In another embodiment, the invention features a compound of formula I-D and the attendant définitions, wherein X is -PO(O‘)₂*D²⁺ and D²⁺ is Mg²⁺, Ca²⁺ or Ba²⁺. In one embodiment, X is -PO(O’)₂«D²⁺ and

D²⁺ is Mg²⁺. In another embodiment, X is -PO(O')2’D²⁺ and D²⁺ is Ca²⁺. In another embodiment, X is PO(O')2«D²⁺ and D²⁺ is Ba²⁺.

In another embodiment, the invention features a compound of formula I-D and the attendant définitions, wherein X is -PO(OH)₂.

In another embodiment, the invention provides a compound of formula I-E

O rV^N^O-X

I-E

Page 18 of 100 wherein, independently for each occurrence:

R² and R³ are independently halogen, or Ci-C₆ alkyl wherein said W alkyl is substituted with 0-6 halogen;

R⁷ is halogen, or Ci-Cô alkyl wherein said Ci-Cé alkyl is substituted with 0-6 halogen and wherein up to two non-adjacent CH2 units of said Ci-Cô alkyl may be replaced with -O-; and

X is -PO(OH)2, -PO(OH)O‘M⁺, -PO(O')2*2M⁺, or -PO(O')2*D²⁺; M⁺ is a pharmaceutically acceptable monovalent cation; and D²⁺ is a pharmaceutically acceptable divalent cation.

In another embodiment, the invention features a compound of formula I-E and the attendant définitions, wherein R² is halogen. In another embodiment, R² is Cl.

In another embodiment, the invention features a compound of formula I-E and the attendant définitions, wherein R³ is halogen. In another embodiment, R³ is Cl.

In another embodiment, the invention features a compound of formula I-E and the attendant définitions, wherein R⁷ is halogen. In one embodiment, R⁷ is F.

In another embodiment, the invention features a compound of formula I-E and the attendant définitions, wherein X is -PO(OH)₂, -PO(OH)OM⁺; -ΡΟ(Ο’)2·2Μ⁺ or -PO(O’)2*D²⁺; wherein M⁺ is Li⁺, Na⁺ or K⁺and wherein D²⁺ is Mg²⁺ or Ca²⁺.

In another embodiment, the invention features a compound of formula I-E and the attendant définitions, wherein R² and R³ are Cl and R⁷ is F.

In another embodiment, the invention features a compound of formula I-E and the attendant définitions, wherein R² and R³ are Cl, R⁷ is F and X is -PO(OH)2In another embodiment, the invention features a compound of formula I-E and the attendant définitions, wherein X is -PO(OH)O’M⁺, -PO(OW; or -PO(O’)2-D²⁺; M⁺ is Li⁺, Na⁺, K⁺ or N(R⁹)4⁺; wherein each R⁹ is independently H or a Ci-C₄ alkyl group and D²⁺ is Mg²⁺, Ca²⁺ or Ba²⁺.

In another embodiment, the invention features a compound of formula I-E and the attendant définitions, wherein X is -PO(OH)O’M⁺ and M⁺ is Li⁺. In one embodiment, X is -PO(OH)O’M⁺ and M⁺ is Na⁺. In another embodiment, X is -PO(OH)O'M⁺ and M⁺ is K⁺. In another embodiment, X is -PO(OH)O’M⁺ and M⁺ is N(R⁹)4⁺; wherein each R⁹ is independently H or a C1-C4 alkyl group. In another embodiment, X is -PO(OH)O’M⁺ and M* is N(R⁹)4⁺; wherein each R⁹ is a CH₃ group.

In another embodiment, the invention features a compound of formula I-E and the attendant définitions, wherein X is -ΡΟ(Ο')₂·2Μ⁺ and M⁺ is Li⁺. In one embodiment, X is -PO(O’)2’2M⁺ and M⁺ is Na⁺. In another embodiment, X is -ΡΟ(Ο)2·2Μ⁺ and M⁺ is K⁺. In another embodiment, X is -PO(O')2*2M⁺ and M⁺ is N(R⁹)4⁺; wherein each R⁹ is independently H or a C1-C4 alkyl group. In another embodiment, X is -PO(O‘)2’2M⁺ and M⁺ is N(R⁹)4⁺; wherein each R⁹ is a CH3 group.

Page 19 of 100

In another embodiment, the invention features a compound of formula I-E and the attendant définitions, wherein X is -PO(O’)2*D²⁺ and D²⁺ is Mg²⁺, Ca²⁺ or Ba²⁺. In one embodiment, X is -PO(O)2’D²⁺ and D²⁺ is Mg²⁺. In another embodiment, X is -PO(O‘)2*D²⁺ and D²⁺ is Ca²⁺. In another embodiment, X is PO(O')₂«D²⁺ and D²⁺ is Ba²⁺.

In another embodiment, the invention features a compound of formula I-E and the attendant définitions, wherein X is -PO(OH)₂.

In another embodiment, the invention provides a compound of formula I-F

wherein, independently for each occurrence:

R² is halogen, or Ci-Cg alkyl wherein said Ci-C₆ alkyl is substituted with 0-6 halogen;

R⁵ is halogen, OH, or Ci-Cg alkyl wherein said Ci-Cg alkyl is substituted with 0-6 halogen and wherein up to two non-adjacent CH₂ units of said Ci-C₆ alkyl may be replaced with -O-; and

X is -PO(OH)₂, -PO(OH)O'M⁺, -PO(O')2*2M⁺, or -PO(O')2*D²⁺; M⁺ is a pharmaceutically acceptable monovalent cation; and D²⁺ is a pharmaceutically acceptable divalent cation.

In another embodiment, the invention features a compound of formula I-F and the attendant définitions, wherein R² is CrC6 alkyl wherein said Ci-C6 alkyl is substituted with 0-6 halogen. In another embodiment, R² is CF3.

In another embodiment, the invention features a compound of formula I-F and the attendant définitions, wherein R⁵ is Cj-Cô alkyl wherein said Ci-Cô alkyl is substituted with 0-6 halogen and wherein up to two non-adjacent CH2 units of said C]-C6 alkyl may be replaced with -O-. In one embodiment, R⁵ is CH3. In another embodiment, R⁵ is OCF₃.

Tn another embodiment, the invention features a compound of formula I-F and the attendant définitions, wherein X is -PO(OH)₂, -PO(OH)O'M⁺; -ΡΟ(Ο’)2·2Μ⁺ or -PO(O')2«D²⁺; wherein M⁺ is Li⁺, Na⁺ or K⁺and wherein D²⁺ is Mg²⁺ or Ca²⁺.

Tn another embodiment, the invention features a compound of formula I-F and the attendant définitions, wherein R² is CF3, R⁷ is CH3 or OCF₃ and X is -PO(OH)₂.

Tn another embodiment, the invention features a compound of formula I-F and the attendant définitions, wherein X is -PO(OH)O‘M⁺, -PO(O‘)2*2M⁺; or -PO(O')2«D²⁺; M⁺ is Li⁺, Na⁺, K⁺ or N(R⁹)₄ ⁺; wherein each R⁹ is independently H or a C1-C4 alkyl group and D²⁺ is Mg²⁺, Ca²⁺ or Ba²⁺.

Page 20 of 100

In another embodiment, the invention features a compound of formula I-F and the attendant définitions, wherein X is -PO(OH)O‘M⁺ and M⁺ is Li⁺. In one embodiment, X is -PO(OH)O'M⁺ and M⁺ is Na⁺. In another embodiment, X is -PO(OH)O'M⁺ and M⁺ is K⁺. In another embodiment, X is -PO(OH)O’M⁺ and M⁺ is NÇR⁹)/; wherein each R⁹ is independently H or a C1-C4 alkyl group. In another embodiment, X is -PO(OH)O‘M⁺ and M⁺ is N(R⁹)4⁺; wherein each R⁹ is a CH3 group.

In another embodiment, the invention features a compound of formula I-F and the attendant définitions, wherein X is -PO(O’)2*2M⁺ and M⁺ is Li⁺. In one embodiment, X is -PO(O‘)2*2M⁺ and M⁺ is Na⁺. In another embodiment, X is -PO(O)2*2M⁺ and M⁺ is K⁺. In another embodiment, X is -PO(O’)2*2M⁺ and M⁺ is N(R⁹)4⁺; wherein each R⁹ is independently H or a C1-C4 alkyl group. In another embodiment, X is -ΡΟ(Ο’)2·2Μ⁺ and M^4- is N(R⁹)4⁺; wherein each R⁹ is a CH₃ group.

In another embodiment, the invention features a compound of formula I-F and the attendant définitions, wherein X is -PO(O')₂’D²⁺ and D²⁺ is Mg²⁺, Ca²⁺ or Ba²⁺. In one embodiment, X is -PO(O’)2’D²⁺ and D²⁺ is Mg²⁺. In another embodiment, X is -PO(O)2*D²⁺ and D²⁺ is Ca²⁺. In another embodiment, X is PO(O‘)2*D²⁺ and D²⁺ is Ba²⁺.

In another embodiment, the invention features a compound of formula I-F and the attendant définitions, wherein X is -PO(OH)₂.

In another embodiment, the invention provides a compound of formula I-G

R⁷

I-G wherein, independently for each occurrence:

R² and R³ are independently halogen, or Ci-C₆ alkyl wherein said Ci-C₆ alkyl is substituted with 0-6 halogen;

R⁵ is halogen, OH, or C^Cô alkyl wherein said Ci-C₆ alkyl is substituted with 0-6 halogen and wherein up to two non-adjacent CH₂ units of said CrC₆ alkyl may be replaced with -O-;

X is -PO(OH)₂, -PO(OH)O’M⁺, -PO(O~)2*2M⁺, or -PO(O')2’D²⁺; M⁺ is a pharmaceutically acceptable monovalent cation; and D²⁺ is a pharmaceutically acceptable divalent cation.

Tn another embodiment, the invention features a compound of formula I-G and the attendant définitions, wherein R² is halogen. In another embodiment, R² is Cl.

Page 21 oflOO

In another embodiment, the invention features a compound of formula I-G and the attendant définitions, wherein R³ is halogen. In another embodiment, R³ is Cl.

In another embodiment, the invention features a compound of formula I-G and the attendant définitions, wherein R⁵ is halogen. In another embodiment, R⁵ is Cl. In another embodiment, R⁵ is F. In another embodiment, R⁵ is Cj-Cg alkyl. In another embodiment, R⁵ is CH3. In another embodiment, R⁵ is Ci-C6 alkyl wherein said Ci-C₆ alkyl is substituted with 0-6 halogen wherein one CH2 unit of said Ci-Cg alkyl is replaced with -O-. In another embodiment, R⁵ is OCH3. In another embodiment, R⁵ is OH. In another embodiment, R⁵ is OCF3.

In another embodiment, the invention features a compound of formula I-G and the attendant définitions, wherein R⁷ is halogen. In one embodiment, R⁷ is F.

In another embodiment, the invention features a compound of formula I-G and the attendant définitions, wherein X is -PO(OH)₂, -PO(OH)O’M⁺; -PO(O)2*2M⁺ or -PO(O’)2’D²⁺; wherein M⁺ is Li⁺, Na⁺ or K⁺and wherein D²⁺ is Mg²⁺ or Ca²⁺.

In another embodiment, the invention features a compound of formula I-G and the attendant définitions, wherein R² and R³ are Cl, R⁵ is OCH₃ and R⁷ is F.

In another embodiment, the invention features a compound of formula I-G and the attendant définitions, wherein R² and R³ are Cl, R⁷ is F, R⁵ is OCH₃ and X is -PO(OH)₂.

In another embodiment, the invention features a compound of formula I-G and the attendant définitions, wherein X is -PO(OH)O'M⁺, -ΡΟ(Ο')2·2Μ⁺; or-PO(O‘)2*D²⁺; M⁺ is Li⁺, Na⁺, K⁺ orN(R⁹)₄ ⁺; wherein each R⁹ is independently H or a C1-C4 alkyl group and D²⁺ is Mg²⁺, Ca²⁺ or Ba²⁺.

In another embodiment, the invention features a compound of formula I-G and the attendant définitions, wherein X is -PO(OH)OM⁺ and M⁺ is Li⁺. In one embodiment, X is -PO(OH)O‘M⁺ and M⁺ is Na⁺. In another embodiment, X is -PO(OH)O’M⁺ and M⁺ is K⁺. In another embodiment, X is -PO(OH)O‘M⁺ and M* is N(R⁹)4⁺; wherein each R⁹ is independently H or a CrC₄ alkyl group. In another embodiment, X is -PO(OH)O’M⁺ and M^4- is N(R⁹)4⁺; wherein each R⁹ is a CH3 group.

Tn another embodiment, the invention features a compound of formula I-G and the attendant définitions, wherein X is -PO(O‘)₂’2M⁺ and M⁺ is Li⁺. In one embodiment, X is -PO(O')2*2M⁺ and M⁺ is Na⁺. In another embodiment, X is -PO(O)2*2M⁺ and M⁺ is K⁺. In another embodiment, X is -PO(O’)2*2M⁺ and M⁺ is N(R⁹)4⁺; wherein each R⁹ is independently H or a C1-C4 alkyl group. In another embodiment, X is -PO(O')2*2M⁺ and M⁺ is N(R⁹)4⁺; wherein each R⁹ is a CH3 group.

In another embodiment, the invention features a compound of formula I-G and the attendant définitions, wherein X is -PO(O')2*D²⁺ and D²⁺ is Mg²⁺, Ca²⁺ or Ba²⁺. In one embodiment, X is -PO(O)2*D²⁺ and D²⁺ is Mg²⁺. Tn another embodiment, X is -PO(O’)2’D²⁺ and D²⁺ is Ca²⁺. In another embodiment, X is PO(O‘)2*D²⁺ and D²⁺ is Ba²⁺.

Page 22 oflOO

In another embodiment, the invention features a compound of formula I-G and the attendant définitions, wherein X is -PO(OH)₂.

In another embodiment, the invention features a compound of formula I-G and the attendant définitions, wherein R² is Cl, R³ is Cl, R⁵ is OCH3, R⁷ is F and X is -PO(OH)2.

In another embodiment, the invention features a compound of formula I-G and the attendant définitions, wherein R² is Cl, R³ is Cl, R⁵ is OCH3, R⁷ is F and X is -ΡΟ(Ο’)2·2Μ⁺, M⁺ is Li⁺, Na⁺, K⁺ or N(R⁹)4⁺wherein each R⁹ is independently H or a Ci-C4 alkyl group.

In another embodiment, the invention features a compound of formula I-G and the attendant définitions, wherein R² is Cl, R³ is Cl, R⁵ is OCH3, R⁷ is F and X is-PO(OH)OM⁺, M⁺ is Li⁺, Na⁺, K⁺ or N(R⁹)4⁺wherein each R⁹ is independently H or a C1-C4 alkyl group.

In another embodiment, the invention features a solid form of (4-(2-(4-fluoro-2-methylphenoxy)-4(trifluoromethyl)benzamido)-2-oxopyridin-l (2J7)-yl)methyl dihydrogen phosphate.

In one embodiment, the présent invention provides a free crystalline Form B of compound 9.

In one embodiment, the présent invention provides a crystalline Form B of (4-(2-(4-fluoro-2methylphenoxy)-4-(trifluoromethyl)benzamido)-2-oxopyridin-l (2B)-yl)methyl dihydrogen phosphate.

In another embodiment, the présent invention provides a crystalline Form B of (4-(2-(4-fluoro-2methylphenoxy)-4-(trifluoromethyl)benzamido)-2-oxopyridin-l (277)-yl)methyl dihydrogen phosphate which is characterized by an X-ray powder diffraction pattern (XRPD) comprising at least three approximate peak positions (degrees 2 thêta + 0.2) when measured using Cu Ka radiation, seiected from the group consisting of 4.4, 15.2, 16.4, 18.0, 19.1, 19.3, 19.9, 20.2, 20.5, 21.0, 22.2, 23.5 24.2, 24.8, 26.3, 29.6, 30.1 and 31.3, when the XRPD is collected from about 4 to about 40 degrees 2 thêta (2 0).

In another embodiment, the présent invention provides a crystalline Form B of (4-(2-(4-fluoro-2methylphenoxy)-4-(trifluoromethyl)benzamido)-2-oxopyridin-l (2/Z)-yl)methyl dihydrogen phosphate which is characterized by an X-ray powder diffraction pattern (XRPD) comprising at least three approximate peak positions (degrees 2 thêta + 0.2) when measured using Cu Ka radiation, seiected from the group consisting of 19.3,22.2,23.5, 26.3 and 30.1, when the XRPD is collected from about 4 to about 40 degrees 2 thêta (2 0).

In another embodiment, the présent invention provides a crystalline Form B of (4-(2-(4-fluoro-2methylphenoxy)-4-(trifluoromethyl)benzamido)-2-oxopyridin-l (2//)-yl)methyl dihydrogen phosphate characterized by an X-ray powder diffraction pattern, as measured by Cu K_a radiation, substantially similar to Figure 2.

In another embodiment, the présent invention provides a crystalline Form B of (4-(2-(4-fIuoro-2methylphenoxy)-4-(trifluoromethyl)benzamido)-2-oxopyridin-l (277)-yl)methyl dihydrogen phosphate, further characterized by an endothermie peak having an onset température at about 210°C degrees as

Page 23 of 100 measured by differential scanning calorimetry in which the température is scanned at about 10°C per minute.

In another embodiment, the présent invention provides a process for preparing solid Form B of compound 9.

In another embodiment, the présent invention provides a process for preparing crystalline Form B of compound 9.

In one embodiment of the process, a substantially pure solid Form B of compound 9 may be prepared from amorphous or crystalline compound 9 by contacting the compound with an organic solvent, a mixture of organic solvents or a mixture of an organic solvent and water at a suitable température, stirring for up to 4 weeks and isolating the solid.

In another embodiment of the process, a substantially pure solid Form B of compound 9 may be prepared from amorphous or crystalline compound 9 by contacting the compound with water at a suitable température, stirring for up to 4 weeks and isolating the solid.

In another embodiment of the process, a substantially pure solid Form B of compound 9 may be prepared from amorphous or crystalline compound 9 by contacting the compound with an alcoholic solvent at room température, stirring for up to 4 weeks and isolating the solid. In another embodiment, the alcoholic solvent comprises methanol, éthanol or isopropanol.

In another embodiment of the process, a substantially pure solid Form B of compound 9 may be prepared from amorphous or crystalline compound 9 by contacting the compound with an organic ester solvent at room température, stirring for up to 4 weeks and isolating the solid. In one embodiment, the organic ester solvent comprises ethyl acetate or isopropyl acetate.

In another embodiment of the process, a substantially pure solid Form B of compound 9 may be prepared from amorphous or crystalline compound 9 by contacting the compound with an organic solvent at room température, stirring for up to 4 weeks and isolating the solid. In one embodiment, the organic solvent comprises acetonitrile, acetone, tetrahydrofuran (THF), 2-methyl tetrahydrofuran or methyl ethyl ketone.

In another embodiment, a substantially pure solid Form B of compound 9 may be prepared from amorphous or crystalline compound 9 by contacting the compound with a mixture of an organic solvent and water at room température, stirring for up to 4 weeks and isolating the solid. In one embodiment, the organic solvent/water mixture comprises THF/water, acetone/water or alcohol/water. In one embodiment, the alcohol ot the alcohol/water mixture comprises, methanol, éthanol or isopropanol.

In another embodiment, a substantially pure solid Form B of compound 9 may be prepared from amorphous or crystalline compound 9 by contacting the compound with a mixture of an organic solvent and water at room température, stirring for up to 4 weeks and isolating the solid.

In another embodiment, a substantially pure solid Form B of compound 9 may be prepared from amorphous or crystalline compound 9 by contacting the compound with a mixture of an organic solvent and water at elevated température, stirring for up to 4 weeks and isolating the solid. In another embodiment, a substantially pure solid Form B of compound 9 may be prepared from amorphous or

Page 24 oflOO crystalline compound 9 by contacting the compound with a mixture of an organic solvent and water at reflux, stirring for up to 24 hours and isolating the solid. In one embodiment, the organic solvent/water mixture comprises THF/water, acetone/water or alcohol/water. In one embodiment, the organic solvent/water mixture comprises acetone/water. In another embodiment, the organic solvent/water mixture comprises THF/water.

In another embodiment, a substantially pure solid Form B of compound 9 may be prepared from amorphous or crystalline compound 9 by exposing the compound to atmospheric conditions for up to 4 weeks and isolating the solid.

In one embodiment of the process, a substantially pure crystalline Form B of compound 9 may be prepared from amorphous or crystalline compound 9 by contacting the compound with an organic solvent, a mixture of organic solvents or a mixture of an organic solvent and water at a suitable température, stirring for up to 4 weeks and isolating the crystalline solid.

In another embodiment of the process, a substantially pure crystalline Form B of compound 9 may be prepared from amorphous or crystalline compound 9 by contacting the compound with water at a suitable température, stirring for up to 4 weeks and isolating the crystalline solid.

In another embodiment of the process, a substantially pure crystalline Form B of compound 9 may be prepared from amorphous or crystalline compound 9 by contacting the compound with an alcoholic solvent at room température, stirring for up to 4 weeks and isolating the crystalline solid. In another embodiment, the alcoholic solvent comprises methanol, éthanol or isopropanol.

In another embodiment of the process, a substantially pure crystalline Form B of compound 9 may be prepared from amorphous or crystalline compound 9 by contacting the compound with an organic ester solvent at room température, stirring for up to 4 weeks and isolating the crystalline solid. In one embodiment, the organic ester solvent comprises ethyl acetate or isopropyl acetate.

In another embodiment of the process, a substantially pure crystalline Form B of compound 9 may be prepared from amorphous or crystalline compound 9 by contacting the compound with an organic solvent at room température, stirring for up to 4 weeks and isolating the crystalline solid. In one embodiment, the organic solvent comprises acetonitrile, acetone, tetrahydrofuran (THF), 2-methyl tetrahydrofuran or methyl ethyl ketone.

In another embodiment, a substantially pure crystalline Form B of compound 9 may be prepared from amorphous or crystalline compound 9 by contacting the compound with a mixture of an organic solvent and water at room température, stirring for up to 4 weeks and isolating the crystalline solid. In one embodiment, the organic solvent/water mixture comprises THF/water, acetone/water or alcohol/water. In one embodiment, the alcohol ot the alcohol/water mixture comprises, methanol, éthanol or isopropanol. Tn another embodiment, a substantially pure crystalline Form B of compound 9 may be prepared from amorphous or crystalline compound 9 by contacting the compound with a mixture of an organic solvent and water at room température, stirring for up to 4 weeks and isolating the crystalline solid.

Page 25 oflOO

In another embodiment, a substantially pure crystalline Form B of compound 9 may be prepared from amorphous or crystalline compound 9 by contacting the compound with a mixture of an organic solvent and water at elevated température, stirring for up to 4 weeks and isolating the solid. Tn another embodiment, a substantially pure crystalline Form B of compound 9 may be prepared from amorphous or crystalline compound 9 by contacting the compound with a mixture of an organic solvent and water at reflux, stirring for up to 24 hours and isolating the crystalline solid. In one embodiment, the organic solvent/water mixture comprises THF/water, acetone/water or alcohol/water. In one embodiment, the organic solvent/water mixture comprises acetone/water. In another embodiment, the organic solvent/water mixture comprises THF/water.

In another embodiment, a substantially pure crystalline Form B of compound 9 may be prepared from amorphous or crystalline compound 9 by exposing the compound to atmospheric conditions for up to 4 weeks and isolating the solid.

Crystalline Form B of compound 9 may be identifîed by a broad endotherm at about 214°C, followed by an exothermic peak at about 217°C. The endothermie peak has an onset température of 210°C. A person skilled in the art would recognize that the peak and onset températures of the endothermie and the endotherms may vary depending on the experimental conditions. Crystalline Form B of compound 9 may also be identifîed by an X-ray powder diffraction pattern essentially as shown in Table 1 and Figure 2 wherein the XRPD patterns were measured using a powder diffractometer equipped with a Cu X-ray tube source. The sample was illuminated with Cu Και radiation and XRPD data were collected from about 4 to about 40° 2 thêta (2 Θ). A person skilled in the art would recognize that relative intensities of the XPRD peaks may significantly vary depending on the orientation of the sample under test and on the type and setting of the instrument used, so that the intensities in the XPRD traces included herein are to such extent illustrative and are not intended to be used for absolute comparisons.

Figure 2 is an X-ray powder diffraction pattern of crystalline Form B of compound 9 collected from about 4 to about 40 degrees 2 Θ. The peaks corresponding to the X-ray powder diffraction pattern having a relative intensity greater than or equal to 5% are listed in Table 1.

Figure 3 shows a DSC thermogram of crystalline Form B of compound 9 exhibiting a broad endothermie peak at about 214°C followed by exothermic peak at about 217°C. The endothermie peak has an onset température of210°C. A person skilled in the art would recognize that the peak and onset températures of the endotherms may vary depending on the experimental conditions. Data in Figure 3 was collected as follows: a sample of approximately 1-2 mg was weighed into an aluminum pan that was crimped using lids with either one pin-hole lids. The DSC sample was scanned from 25°C to températures indicated in the plots at a heating rate of 10°C/min with 50 mL/min nitrogen flow. The samples run under modulated DSC (MDSC) were modulated + and - 1°C every 60 seconds with ramp rates of 2 or 3 °C/min. Data was collected and analyzed by TRIOS (TA Instruments, New Castle, DE)

Page 26 oflOO

Figure 4 is a TGA (thermal gravimétrie analysis) thermogram of crystalline Form B of compound 9 exhibiting an onset weight loss at about 218°C and scanned from room température to about 300°C at a heating rate of 10°C/min.

In one embodiment, the présent invention provides a solid Form B of (4-(2-(4-fluoro-2-methylphenoxy)4-(trifluoromethyl)benzamido)-2-oxopyridin-l (227)-yl)methyl dihydrogen phosphate.

In one embodiment, the présent invention provides a crystalline Form B of (4-(2-(4-fluoro-2methylphenoxy)-4-(trifluoromethyl)benzamido)-2-oxopyridin-l (277)-yl)methyl dihydrogen phosphate.

In another embodiment, the crystalline Form B of (4-(2-(4-fluoro-2-methylphenoxy)-4(trifluoromethyl)benzamido)-2-oxopyridin-l(2/7)-yl)methyl dihydrogen phosphate is characterized by an X-ray powder diffraction pattern (XPRD) comprising at least three approximate peak positions (degrees 2 thêta [2 Θ] ± 0.2) when measured using Cu Ka radiation, selected from the group consisting of 4.4, 12.7,

13.3, 14.7, 15.2, 16.4, 18.0, 19.1, 19.3, 19.9, 20.2, 20.5, 21.0, 22.2, 23.5 24.2, 24.8, 26.3, 29.6, 30.1 and

31.3, when the XPRD is collected from about 4 to about 40 degrees 2 thêta (2 0).

In another embodiment, the crystalline Form B of (4-(2-(4-fhioro-2-methylphenoxy)-4(trifluoromethyl)benzamido)-2-oxopyridin-l(27ï)-yl)methyl dihydrogen phosphate is characterized by an X-ray powder diffraction pattern (XPRD) comprising at least three approximate peak positions (degrees 2 thêta ± 0.2) when measured using Cu Ka radiation, selected from the group consisting of 4.4, 16.4,

19.3, 22.2, 23.5, 26.3, 29.6 and 30.1 when the XPRD is collected from about 4 to about 40 degrees 2 0.

In another embodiment, the crystalline Form B of (4-(2-(4-fluoro-2-methylphenoxy)-4(trifluoromethyl)benzamido)-2-oxopyridin-l(217)-yl)methyl dihydrogen phosphate is characterized by an X-ray powder diffraction pattern, as measured using Cu Kx radiation, substantially similar to Figure 2.

In another embodiment, the crystalline Form B of (4-(2-(4-fluoro-2-methylphenoxy)-4(trifluoromethyl)benzamido)-2-oxopyridin-l(277)-yl)methyl dihydrogen phosphate is further characterized by an endothermie peak having an onset température at about 210°C as measured by differential scanning calorimetry in which the température is scanned at 2-3°C per minute.

In another embodiment, the présent invention provides a method for preparing crystalline Form B of compound 9 comprising suspending a solid material of the free form in a solvent system comprising one or more organic solvents or a mixture of one or more organic solvents and water and isolating the solid.

In another embodiment, the présent invention provides a process for preparing solid Form B of compound 9, comprising contacting compound 9 with water, an organic solvent, a mixture of organic solvents or a mixture of an organic solvent and water at a suitable température, stirring for up to 4 weeks and isolating the solid.

In another embodiment, the présent invention provides a process for preparing crystalline Form B of compound 9, comprising direct crystallization from a reaction mixture with or without seeding with Form

B. In one embodiment, the direct crystallization is from a final deprotection step wherein compound 20

Page 27 oflOO is heated to a suitable température in a suitable solvent mixture with a suitable acidic reagent for a suitable period of time. In one embodiment, the suitable organic solvent mixture is water and acetonitrile, the suitable acidic reagent is acetic acid, the suitable température is between 50°C and 100°C and the suitable time is between 10 and 240 minutes.

In one embodiment, the direct crystallization process comprises treating di-ieri-butyl [4-[[2-(4-fluoro-2methyl-phenoxy)-4-(trifhioromethyl)benzoyl]amino]-2-oxo-l-pyridyl]methyl phosphate (123.2 g, 196 mmole) in a 3 liter flask with acetonitrile (1.23 L), acetic acid (616 ml) water (616 ml) and heating with stirring at 70°C for 1.1 hours, then treating the mixture with a few seed crystals of Form B of 4-(2-(4fluoro-2-methylphenoxy)-4-(trifluoromethyl)benzamido)-2-oxopyridin-l(27ï)-yl)methyl dihydrogen phosphate, letting the mixture stir at 70°C until cloudy, then tuming off the heat and allowing the mixture to cool to room température. After stirring ovemight, crystalline solids were collected by filtration and dried in a vaccum oven to constant weight to give 84.0 grams of crystalline Form B of 4-(2-(4-fluoro-2methylphenoxy)-4-(trifluoromethyl)benzamido)-2-oxopyridin-l (277)-yl)methyl dihydrogen phosphate. In another embodiment, crystalline Form B is chemically and physically stable for at least one month at 5°C/dry, 40°C/dry, 25°C with relative humidity of up to 60%; and 40°C with relative humidity of up to 75%. In another embodiment, crystalline Form B is chemically and physically stable for at least three months in ail conditions including, but not limited to open dish at 40°C, 40°C with relative humidity of up to 75%, 25°C with relative humidity of up to 60% and closed dish at 5°C with a desiccator. Chemically and physically stable means no changes were observed on the X-ray powder diffraction and HPLC impurity profiles (e.g., less than 0.2% variance) and there were no observed changes in physical appearance of the samples.

Table 1. XRPD pattern peaks for crystalline Form B of 4-(2-(4-fluoro-2-methylphenoxy)-4(trifluoromethyl)benzamido)-2-oxopyridin-l (2Z/)-yl)methyl dihydrogen phosphate

Peak No.	Position [°20]	Relative Intensity [%1
1	4.43	13.6
2	12.74	2.3
3	13.29	4.5
4	14.70	1.9
5	15.19	8.6
6	16.42	13.9
7	17.99	5.3
8	19.12	10.4
9	19.34	15.8
10	19.85	8.8
11	20.19	11.1
12	20.45	12.3
13	21.03	11.2
14	22.20	100.0
15	23.52	30.6
16	24.21	11.5
17	24.81	11.7

Page 28 oflOO

Peak No.	Position [°2θ]	Relative Intensity [%]
18	26.33	21.2
19	29.59	13.1
20	30.05	15.8
21	31.28	10.5

In one embodiment, the présent invention provides an amorphous Form C of (4-(2-(4-fluoro-2methylphenoxy)-4-(trifhioromethyl)benzamido)-2-oxopyridin-l (2/7)-yl)methyl dihydrogen phosphate.

In another embodiment, the amorphous Form C of compound 9 is characterized by an X-ray powder diffraction pattern (XPRD) using Cu Ka radiation, characterized by a broad halo with no discernable diffraction peak.

In yet another embodiment, the présent invention provides a method for preparing amorphous Form C of compound 9 comprising spray drying a solution of compound 9 with or without co-polymer.

In one embodiment, the présent application provides a process for preparing solid Form C of the compound 9. In some embodiments, the amorphous material is collected after being precipitated from a solvent or from a solution after concentrating the solution by evaporating some of the solvent, for example, using a rotator evaporator. Alternatively, adding a second solvent to the mixture may precipitate Form C.

Compound 9 may be converted to amorphous solid Form C using any method known to those skilled in the art. The amorphous compound 9 may be characterized by the absence of a diffraction pattern characteristic of a crystalline form. The X-ray powder diffraction of a partially amorphous Form C may still lack features characteristic of a crystal form because the diffraction peaks from the crystalline portion of the sample may be too weak to be observable over the noise. Figure 5 is an X-ray powder diffraction pattern of an amorphous Form X of compound 9.

In one embodiment, the amoiphous Form C of compound 9 may be prepared by spray drying a solution of the compound in appropriate solvent. Spray drying is well known in the art and is often used to dry thermally-sensitive materials such as pharmaceutical drugs. Spray drying also provides consistent particle distribution that can be reproduced fairly well. Any gas may be used to dry the powder although air is commonly used. If the material is sensitive to air, an inert gas, such nitrogen or argon, may be used. Any method that couverts a solution, slurry, suspension or an émulsion of the compound to produce a solid powder may be suitable for amoiphous Form C of compound 9.

In one embodiment, a solution of compound 9 in a polar solvent may be spray dried using a nanospray dryer equipped a condenser. The inlet température may be kept between 70-120°C.

It is to be understood that crystalline Form B of compound 9 and amorphous solid Form C of compound 9, in addition to having the XRPD, DSC, TGA and other characteristics described herein, may also possess other characteristics not described, such as but not limited to the presence of water or one or more solvent molécules.

Page 29 oflOO

X-Ray Powder Diffraction (XRPD): The powder x-ray diffraction measurements were performed using PANalytical’s X-pert Pro diffractometer at room température with copper radiation (1.54060 A). The incident beam optic was comprised of a variable divergence slit to ensure a constant illuminated length on the sample and on the diffracted beam side; a fast linear solid state detector was used with an active length of 2.12 degrees 2 thêta measured in a scanning mode. The powder sample was packed on the indented area of a zéro background silicon holder and spinning was performed to achieve better statistics. A symmetrical scan was measured from 4-40 degrees 2 thêta (2 Θ) with a step size of 0.017 degrees and a scan step time of 15.5s.

Differential Scanning Calorimetry (DSC):

DSC was performed on a sample of the material using a Discovery DSC differential scanning calorimeter (TA Instruments, New Castle, DE). The instrument was calibrated with indium. A sample of approximately 1-2 mg was weighed into an aluminum pan that was crimped using lids with either one pin-hole lids. The DSC samples were scanned from 25°C to températures indicated in the plots at a heating rate of 10°C/min with 50 mL/min nitrogen flow. The samples run under modulated DSC (MDSC) were modulated + and - 1°C every 60s with ramp rates of 2 or 3 °C/min. Data was collected and analyzed by TRIOS (TA Instruments, New Castle, DE).

Thermogravimetric analysis (TGA):

A Model Discovery TGA, Thermogravimetric Analyzer (TA Instruments, New Castle, DE) was used for TGA measurement. A sample with weight of approximately 2-5 mg was scanned from room température to températures indicated on the plots at a heating rate of 10°C/min. Data was collected and analyzed by TRIOS software (TA Instruments, New Castle, DE).

Compounds names in the présent invention were generated using ChemBioDrawUltra version 12.0 from Cambridge Soft/Chem Office 2010.

Table 2 Compound Numbers, Structures and Chemical Names

Page 30 oflOO

1	Q> OH YOH r° Λ·/-' ΗΝ,ζΟ OMe YM M Cl (4-(4,5-dichloro-2-(4-fluoro-2methoxyphenoxy)benzamido)-2oxopyridin-1 (2Æ)-yI)methyl dihydrogen phosphate
2	Ox /OH 1 ΌΗ r° HN./O OMe ,Y”O Lf f₃c^f (4-(2-(4-fluoro-2methoxyphenoxy)-4(perfluoroethyl)benzamido)-2oxopyridin-1 (2H)-yl)methyl dihydrogen phosphate

3	OH ΓΌΗ r° V ΗΝ^,Ο Cl (4-(4,5-dichloro-2-(4fluorophenoxy)benzamido)-2oxopyridin-1 (227)-yl)methyl dihydrogen phosphate
4	°v^0H ΓΌΗ (° çr° ΗΝχ^.0 ,ΧΧϊΧ (4-(2-(4-fluoro-2methoxyphenoxy)-5 (trifluoromethyl)benzamido)-2oxopyridin-1 (277)-yl)methyl dihydrogen phosphate
5	Y_V ^0HΓΟΗ r° HN^.0 γγφ cf₃(2-oxo-4-(2-(4-

Page 31 oflOO

	(trifluoromethoxy)phenoxy)-4(trifluoromethyl)benzamido)pyridi n-l(277)-yl)methyl dihydrogen phosphate
6	OH AoH r° /V g ΗΝχ^,Ο A Lf f₃c^Af (4-(2-(4-fluorophenoxy)-4(perfluoroethyl)benzamido)-2oxopyridin-l(2ZZ)-yl)methyl dihydrogen phosphate
7	O_V ^OHAoH r° /^NV° ζχ HN^^O OMe AA (4-(5-chloro-2-(4-fluoro-2methoxyphenoxy)benzamido)-2oxopyridin-1 (277)-yl)methyl dihydrogen phosphate

8	1 OH r° HN^.0 xrA (2-oxo-4-(2-(4- (trifluoromethoxy)phenoxy)-5(trifluoromethyl)benzamido)pyridi n-l(277)-yl)methyl dihydrogen phosphate
9	°v^0H Aoh r° xA cf₃ (4-(2-(4-fluoro-2-methylphenoxy)- 4-(trifluoromethyl)benzamido)-2oxopyridin-1 (277)-yl)methyl dihydrogen phosphate
10	<V°^H XOH r° g HN^O (4-(2-(4-fluoro-2-methylphenoxy)- 5-(trifluoromethyl)benzamido)-2-

Page 32 oflOO

	oxopyridin-1 (277)-yl)methyl dihydrogen phosphate
11	0,_V ^0H Y Y ΗΝχ^Ο A (4-(2-(2-chloro-4-fluorophenoxy)5-(trifluoromethyl)benzamido)-2oxopyridin-1 (277)-yl)methyl dihydrogen phosphate
12	°V^0HYOH r° Me V (4-(5-chloro-2-(4-fluoro-2methylphenoxy)benzamido)-2oxopyridin-l(2F7)-yl)methyl dihydrogen phosphate
13	CK /OH IOH r° <^NV° HN^.0 Cl (4-(4-chloro-2-(4-fluoro-2methylphenoxy)benzamido)-2-

	oxopyridin-1 (27¥)-yl)methyl dihydrogen phosphate
14	0 ^0H Y HN. Γ3 Cl A ,Υά, (4-(5-chloro-2-(2-chloro-4fluorophenoxy)benzamido)-2oxopyridin-1 (2Z/)-yl)methyl dihydrogen phosphate
15	°V^OH o^X°^H 4 HN^.0 col (2-oxo-4-(2-(o-tolyloxy)-5 (trifluoromethyl)benzamido)pyridi n-l(2//)-yl)methyl dihydrogen phosphate
16	<V°^H ΓΌΗ r° HN^.0 cf₃ (4-(2-(2,4-difluorophenoxy)-4-

Page 33 oflOO

	(trifluoromethyl)benzamido)-2oxopyridin-1 (2ZZ)-yl)inethyl dihydrogen phosphate
17	HN^.0 ocf₃ Y Αά, (2-oxo-4-(2-(2- (trifluoromethoxy)phenoxy)-5- (trifluoromethyl)benzamido)pyridi

	n-l(277)-yl)methyl dihydrogen phosphate
18	<V^0H A (4-(2-(4-fluorophenoxy)-5 (trifluoromethyl)benzamido)-2oxopyridin-1 (27Z)-yl)methyl dihydrogen phosphate

Page 34 oflOO

In one embodiment, the compound is (4-(4,5-dichloro-2-(4-fluoro-2-methoxyphenoxy)benzamido)-2oxopyridin-l(27/)-yl)methyl dihydrogen phosphate or a pharmaceutically acceptable sait thereof.

Ih another embodiment, the compound is (4-(2-(4-fluoro-2-methoxyphenoxy)-4(perfluoroethyl)benzamido)-2-oxopyridin-l(277)-yl)methyl dihydrogen phosphate or a pharmaceutically acceptable sait thereof.

In another embodiment, the compound is (4-(4,5-dichloro-2-(4-fluorophenoxy)benzamido)-2-oxopyridinl(277)-yl)methyl dihydrogen phosphate or a pharmaceutically acceptable sait thereof.

In another embodiment, the compound is (4-(2-(4-fluoro-2-methoxyphenoxy)-5(trifluoromethyl)benzamido)-2-oxopyridin-l(277)-yl)methyl dihydrogen phosphate or a pharmaceutically acceptable sait thereof.

In another embodiment, the compound is (2-oxo-4-(2-(4-(trifluoromethoxy)phenoxy)-4(trifluoromethyl)benzamido)pyridin-l(2Z/)-yl)methyl dihydrogen phosphate or a pharmaceutically acceptable sait thereof.

In another embodiment, the compound is (4-(2-(4-fluorophenoxy)-4-(perfluoroethyl)benzamido)-2oxopyridin-l(277)-yl)methyl dihydrogen phosphate or a pharmaceutically acceptable sait thereof.

In another embodiment, the compound is (4-(5-chloro-2-(4-fluoro-2-methoxyphenoxy)benzamido)-2oxopyridin-l(2J7)-yl)methyl dihydrogen phosphate or a pharmaceutically acceptable sait thereof.

In another embodiment, the compound is (2-oxo-4-(2-(4-(trifluoromethoxy)phenoxy)-5(trifluoromethyl)benzamido)pyridin-l(2//)-yl)methyl dihydrogen phosphate or a pharmaceutically acceptable sait thereof.

In another embodiment, the compound is (4-(2-(4-fluoro-2-methylphenoxy)-4(trifluoromethyl)benzamido)-2-oxopyridin-l(2F7)-yl)methyl dihydrogen phosphate or a pharmaceutically acceptable sait thereof.

In another embodiment, the compound is (4-(2-(4-fluoro-2-methylphenoxy)-5(trifluoromethyl)benzamido)-2-oxopyridin-l(277)-yl)methyl dihydrogen phosphate or a pharmaceutically acceptable sait thereof.

Ih another embodiment, the compound is (4-(2-(2-chloro-4-fluorophenoxy)-5(trifluoromethyl)benzamido)-2-oxopyridin-l(2W)-yl)methyl dihydrogen phosphate or a pharmaceutically acceptable sait thereof.

In another embodiment, the compound is (4-(5-chloro-2-(4-fluoro-2-methylphenoxy)benzamido)-2oxopyridin-l(277)-yl)methyl dihydrogen phosphate or a pharmaceutically acceptable sait thereof.

Tn another embodiment, the compound is (4-(4-chloro-2-(4-fluoro-2-methylphenoxy)benzamido)-2oxopyridin-l(2/7)-yl)methyl dihydrogen phosphate or a pharmaceutically acceptable sait thereof.

Page 35 of 100

In another embodiment, the compound is (4-(5-chloro-2-(2-chloro-4-fluorophenoxy)benzamido)-2oxopyridin-l(27Y)-yl)methyl dihydrogen phosphate or a pharmaceutically acceptable sait thereof.

In another embodiment, the compound is (2-oxo-4-(2-(o-tolyloxy)-5-(trifluoromethyl)benzamido)pyridinl(2Z/)-yl)methyl dihydrogen phosphate or a pharmaceutically acceptable sait thereof.

In another embodiment, the compound is (4-(2-(2,4-difluorophenoxy)-4-(trifluoromethyl)benzamido)-2oxopyridin-l(277)-yl)methyl dihydrogen phosphate or a pharmaceutically acceptable sait thereof.

In another embodiment, the compound is (2-oxo-4-(2-(2-(trifluoromethoxy)phenoxy)-5(trifluoromethyl)benzamido)pyridin-l(2//)-yl)methyl dihydrogen phosphate or a pharmaceutically acceptable sait thereof.

In another embodiment, the compound is (4-(2-(4-fluorophenoxy)-5-(trifluoromethyl)benzamido)-2oxopyridin-l(277)-yl)methyl dihydrogen phosphate or a pharmaceutically acceptable sait thereof.

Compositions, Uses, Formulation,Administration and Additional Agents

Pharmaceutically acceptable compositions

As discussed herein, the invention provides compounds that are inhibitors of voltage-gated sodium channels, and thus the présent compounds are useful for the treatment of diseases, disorders, and conditions including, but not limited to chronic pain, gut pain, neuropathie pain, musculoskeletal pain, acute pain, inflammatory pain, cancer pain, idiopathic pain, multiple sclerosis, Charcot-Marie-Tooth syndrome, incontinence, pathological cough, or cardiac arrhythmia. Accordingly, in another aspect of the invention, pharmaceutically acceptable compositions are provided, wherein these compositions comprise any of the compounds as described herein, and optionally comprise a pharmaceutically acceptable carrier, adjuvant or vehicle. In certain embodiments, these compositions optionally further comprise one or more additional therapeutic agents.

The term “pharmaceutically acceptable sait” refers to those salts which are, within the scope of sound medical judgement, suitable for use in contact with the tissues of humans and lower animais without undue toxicity, irritation, allergie response and the like, and are commensurate with a reasonable benefit/risk ratio. A “pharmaceutically acceptable sait” means any non-toxic sait of this invention that, upon administration to a récipient, is capable of providing, either directly or indirectly, a compound of this invention or an inhibitorily active métabolite or residue thereof. As used herein, the term “inhibitorily active métabolite or residue thereof’ means that a métabolite or residue thereof is also an inhibitor of a voltage-gated sodium channel.

Pharmaceutically acceptable salts are well known in the art. For example, S. M. Berge, et al. describe pharmaceutically acceptable salts in detail in J. Pharmaceutical Sciences, 1977, 66, 1-19, incorporated

Page 36 of 100 herein by reference. Pharmaceutically acceptable salts of the compounds of this invention include those derived from suitable inorganic and organic acids and bases. Examples of pharmaceutically acceptable, nontoxic acid addition salts are salts of an amino group formed with inorganic acids such as hydrochloric acid, hydrobromic acid, phosphoric acid, sulfuric acid and perchloric acid or with organic acids such as acetic acid, oxalic acid, maleic acid, tartaric acid, citric acid, succinic acid or malonic acid or by using other methods used in the art such as ion exchange. Other pharmaceutically acceptable salts include adipate, alginate, ascorbate, aspartate, benzenesulfonate, benzoate, bisulfate, borate, butyrate, camphorate, camphorsulfonate, citrate, cyclopentanepropionate, digluconate, dodecylsulfate, ethanesulfonate, formate, fumarate, glucoheptonate, glycérophosphate, gluconate, hemisulfate, heptanoate, hexanoate, hydroiodide, 2-hydroxy-ethanesulfonate, lactobionate, lactate, laurate, lauryl sulfate, malate, maleate, malonate, methanesulfonate, 2-naphthalenesulfonate, nicotinate, nitrate, oleate, oxalate, palmitate, pamoate, pectinate, persulfate, 3-phenylpropionate, phosphate, picrate, pivalate, propionate, stéarate, succinate, sulfate, tartrate, thiocyanate, p-toluenesulfonate, undecanoate, valerate salts, and the like. Salts derived from appropriate bases include alkali métal, alkaline earth métal, ammonium and N*(Ομ₄ alkyl)₄ salts. This invention also envisions the quatemization of any basic nitrogen-containing groups of the compounds disclosed herein. Water or oil-soluble or dispersable products may be obtained by such quatemization. Représentative alkali or alkaline earth métal salts include sodium, lithium, potassium, calcium, magnésium, and the like. Further pharmaceutically acceptable salts include, when appropriate, nontoxic ammonium, quatemary ammonium, and amine cations formed using counterions such as halide, hydroxide, carboxylate, sulfate, phosphate, nitrate, lower alkyl sulfonate and aryl sulfonate.

As described herein, the pharmaceutically acceptable compositions of the invention additionally comprise a pharmaceutically acceptable carrier, adjuvant, or vehicle, which, as used herein, includes any and ail solvents, diluents, or other liquid vehicle, dispersion or suspension aids, surface active agents, isotonie agents, thickening or emulsifying agents, preservatives, solid binders, lubricants and the like, as suited to the particular dosage form desired. Remington’s Pharmaceutical Sciences, Sixteenth Edition,

E. W. Martin (Mack Publishing Co., Easton, Pa., 1980) discloses various carriers used in formulating pharmaceutically acceptable compositions and known techniques for the préparation thereof. Except insofar as any conventional carrier medium is incompatible with the compounds of the invention, such as by producing any undesirable biological effect or otherwise interacting in a deleterious manner with any other component(s) of the pharmaceutically acceptable composition, its use is contemplated to be within the scope of this invention. Some examples of materials which can serve as pharmaceutically acceptable carriers include, but are not limited to, ion exchangers, alumina, aluminum stéarate, lecithin, sérum proteins, such as human sérum albumin, buffer substances such as phosphates, glycine, sorbic acid, or

Page 37 oflOO potassium sorbate, partial glyceride mixtures of saturated vegetable fatty acids, water, salts or electrolytes, such as protamine sulfate, disodium hydrogen phosphate, potassium hydrogen phosphate, sodium chloride, zinc salts, colloïdal silica, magnésium trisilicate, polyvinyl pyrrolidone, polyacrylates, waxes, polyethylene-polyoxypropylene-block polymers, wool fat, sugars such as lactose, glucose and sucrose; starches such as corn starch and potato starch; cellulose and its dérivatives such as sodium carboxymethyl cellulose, ethyl cellulose and cellulose acetate; powdered tragacanth; malt; gelatin; talc; excipients such as cocoa butter and suppository waxes; oils such as peanut oil, cottonseed oil; safflower oil; sesame oil; olive oil; corn oil and soybean oil; glycols; such a propylene glycol or polyethylene glycol; esters such as ethyl oleate and ethyl laurate; agar; buffering agents such as magnésium hydroxide and aluminum hydroxide; alginic acid; pyrogen-free water; isotonie saline; Ringer’s solution; ethyl alcohol, and phosphate buffer solutions, as well as other non-toxic compatible lubricants such as sodium lauryl sulfate and magnésium stéarate, as well as coloring agents, releasing agents, coating agents, sweetening, flavoring and perfuming agents, preservatives and antioxidants can also be présent in the composition, according to the judgment of the formulator.

In another aspect, the invention features a pharmaceutical composition comprising the compound of the invention and a pharmaceutically acceptable carrier.

Tn another aspect, the invention features a pharmaceutical composition comprising a therapeutically effective amount of the compounds of formula I and one or more pharmaceutically acceptable carriers or vehicles.

Uses of Compounds and Pharmaceutically Acceptable Compositions

Tn another aspect, the invention features a method of inhibiting a voltage-gated sodium channel in a subject comprising administering to the subject a compound of formula I or a pharmaceutical composition thereof. In another aspect, the voltage-gated sodium channel isNavl.8.

In yet another aspect, the invention features a method of treating or lessening the severity in a subject of chronic pain, gut pain, neuropathie pain, musculoskeletal pain, acute pain, inflammatory pain, cancer pain, idiopathic pain, multiple sclerosis, Charcot-Marie-Tooth syndrome, incontinence, pathological cough, or cardiac arrhythmia comprising administering an effective amount of a compound to the subject, or a phannaceutical composition of the compound of formula I.

Tn yet another aspect, the invention features a method of treating or lessening the severity in a subject of gut pain, wherein gut pain comprises inflammatory bowel disease pain, Crohn’s disease pain or interstitial cystitis pain wherein said method comprises administering an effective amount of a compound, or a pharmaceutical composition of the compound of formula I.

Page 38 oflOO

In yet another aspect, the invention features a method of treating or lessening the severity in a subject of neuropathie pain, wherein neuropathie pain comprises post-herpetic neuralgia, diabetic neuralgia, painful HIV-associated sensory neuropathy, trigeminal neuralgia, burning mouth syndrome, post-amputation pain, phantom pain, painful neuroma, traumatic neuroma, Morton’s neuroma, nerve entrapment injury, spinal stenosis, carpal tunnel syndrome, radicular pain, sciatica pain; nerve avulsion injury, brachial plexus avulsion injury, complex régional pain syndrome, drug therapy induced neuralgia, cancer chemotherapy induced neuralgia, anti-retroviral therapy induced neuralgia, post spinal cord injury pain, idiopathic small-fïber neuropathy, idiopathic sensory neuropathy or trigeminal autonomie cephalalgia wherein said method comprises administering an effective amount of a compound, or a pharmaceutical composition of the compound of formula I.

In yet another aspect, the invention features a method of treating or lessening the severity in a subject of musculoskeletal pain, wherein musculoskeletal pain comprises osteoarthritis pain, back pain, cold pain, bum pain or dental pain wherein said method comprises administering an effective amount of a compound, or a pharmaceutical composition of the compound of formula I.

In yet another aspect, the invention features a method of treating or lessening the severity in a subject of inflammatory pain, wherein inflammatory pain comprises rheumatoid arthritis pain or vulvodynia wherein said method comprises administering an effective amount of a compound, or a pharmaceutical composition of the compound of formula I.

In yet another aspect, the invention features a method of treating or lessening the severity in a subject of inflammatory pain, wherein inflammatory pain comprises rheumatoid arthritis pain wherein said method comprises administering an effective amount of a compound, or a pharmaceutical composition of the compound of formula I.

In yet another aspect, the invention features a method of treating or lessening the severity in a subject of idiopathic pain, wherein idiopathic pain comprises fïbromyalgia pain wherein said method comprises administering an effective amount of a compound, or a pharmaceutical composition of the compound of formula I.

In yet another aspect, the invention features a method of treating or lessening the severity in a subject of pathological cough wherein said method comprises administering an effective amount of a compound, or a pharmaceutical composition of the compound of formula I.

In yet another aspect, the invention features a method wherein the subject is treated with one or more additional therapeutic agents administered concurrently with, prior to, or subséquent to treatment with an effective amount of a compound, or a pharmaceutical composition of the compound of formula I.

Page 39 oflOO

In another aspect, the invention features a method of inhibiting a voltage-gated sodium channel in a subject comprising administering to the subject an effective amount of a compound, or a pharmaceutical composition of the compound of formula I. In another aspect, the voltage-gated sodium channel is Na_v1.8.

In another aspect, the invention features a method of inhibiting a voltage-gated sodium channel in a biological sample comprising contacting the biological sample with an effective amount of a compound, or a pharmaceutical composition of the compound of formula I. In another aspect, the voltage-gated sodium channel is Na_v1.8.

In another aspect, the invention features a method of treating or lessening the severity in a subject of acute pain, chronic pain, neuropathie pain, inflammatory pain, arthritis, migraine, cluster headaches, trigeminal neuralgia, herpetic neuralgia, general neuralgias, epilepsy, epilepsy conditions, neurodegenerative disorders, psychiatrie disorders, anxiety, dépréssion, dipolar disorder, myotonia, arrhythmia, movement disorders, neuroendocrine disorders, ataxia, multiple sclerosis, irritable bowel syndrome, incontinence, pathological cough, viscéral pain, osteoarthritis pain, postherpetic neuralgia, diabetic neuropathy, radicular pain, sciatica, back pain, head pain, neck pain, severe pain, intractable pain, nociceptive pain, breakthrough pain, postsurgical pain, cancer pain, stroke, cérébral ischemia, traumatic brain injury, amyotrophie latéral sclerosis, stress induced angina, exercise induced angina, palpitations, hypertension, or abnormal gastro-intestinal motility, comprising administering an effective amount of a compound, or a pharmaceutical composition of the compound of formula I.

In another aspect, the invention features a method of treating or lessening the severity in a subject of fémur cancer pain, non-malignant chronic bone pain, rheumatoid arthritis, osteoarthritis, spinal stenosis, neuropathie low back pain, myofascial pain syndrome; fibromyalgia, temporomandibular joint pain, chronic viscéral pain, abdominal pain, pancreatic pain, IBS pain, chronic and acute headache pain; migraine, tension headache, cluster headaches, chronic and acute neuropathie pain, post-herpetic neuralgia, diabetic neuropathy, HIV-associated neuropathy, trigeminal neuralgia, Charcot-Marie Tooth neuropathy, hereditary sensory neuropathy, peripheral nerve injury, painful neuromas, ectopic proximal and distal discharges; radiculopathy, chemotherapy induced neuropathie pain, radiotherapy-induced neuropathie pain, post-mastectomy pain, central pain, spinal cord injury pain, post-stroke pain, thalamic pain, complex régional pain syndrome, phantom pain, intractable pain, acute pain, acute post-operative pain, acute musculoskeletal pain, joint pain, mechanical low back pain, neck pain, tendonitis, injury pain, exercise pain, acute viscéral pain, pyelonephritis, appendicitis, cholecystitis, intestinal obstruction, hemias, chest pain, cardiac pain; pelvic pain, rénal colic pain, acute obstetric pain, labor pain; cesarean section pain, acute inflammatory pain, bum pain, trauma pain, acute intermittent pain, endometriosis,

Page 40 of 100 acute herpes zoster pain, sickle cell anémia, acute pancreatitis, breakthrough pain, orofacial pain, sinusitis pain, dental pain, multiple sclerosis (MS) pain, pain in dépréssion, leprosy pain, Behcet’s disease pain, adiposis dolorosa, phlebitic pain, Guillain-Barre pain, painful legs and moving toes; Haglund syndrome, erythromelalgia pain, Fabry’s disease pain, bladder and urogénital disease, urinary incontinence, pathological cough, hyperactivebladder, painful bladder syndrome, interstitial cyctitis (IC) , prostatitis, complex régional pain syndrome (CRPS) type I, complex régional pain syndrome (CRPS),type Π, widespread pain, paroxysmal extreme pain, pruritis, tinnitis, or angina-induced pain, comprising administering an effective amount of a compound, or a pharmaceutical composition of the compound of formula I.

In another aspect, the invention features a method of treating or lessening the severity in a subject of neuropathie pain comprising administering an effective amount of a compound, or a pharmaceutical composition of the compound of formula I. In one aspect, the neuropathie pain is selected from postherpetic neuralgia, diabetic neuralgia, painful HIV-associated sensory neuropathy, trigeminal neuralgia, buming mouth syndrome, post-amputation pain, phantom pain, painful neuroma, traumatic neuroma, Morton’s neuroma, nerve entrapment injury, spinal stenosis, carpal tunnel syndrome, radicular pain, sciatica pain, nerve avulsion injury, brachial plexus avulsion, complex régional pain syndrome, drug therapy induced neuralgia, cancer chemotherapy induced neuralgia, anti-retroviral therapy induced neuralgia, post spinal cord injury pain, idiopathic small-fiber neuropathy, idiopathic sensory neuropathy or trigeminal autonomie cephalalgia.

Manufacture of Médicaments

Tn one aspect, the invention provides the use of a compound or pharmaceutical composition of formula I for the manufacture of a médicament for use in inhibiting a voltage-gated sodium channel. In another aspect, the voltage-gated sodium channel is Na_v1.8.

In yet another aspect, the invention provides the use of a compound or pharmaceutical composition of formula I for the manufacture of a médicament for use in treating or lessening the severity in a subject of chronic pain, gut pain, neuropathie pain, musculoskeletal pain, acute pain, inflammatory pain, cancer pain, idiopathic pain, multiple sclerosis, Charcot-Marie-Tooth syndrome, incontinence, pathological cough, or cardiac arrhythmia.

Tn yet another aspect, the invention provides the use of a compound or pharmaceutical composition of formula I for the manufacture of a médicament for use in treating or lessening the severity in a subject of gut pain, wherein gut pain comprises inflammatory bowel disease pain, Crohn’s disease pain or interstitial cystitis pain.

Page 41 of 100

In yet another aspect, the invention provides the use of a compound or pharmaceutical composition of formula I for the manufacture of a médicament for use in a treating or lessening the severity in a subject of neuropathie pain, wherein neuropathie pain comprises post-herpetic neuralgia, diabetic neuralgia, painful HIV-associated sensory neuropathy, trigeminal neuralgia, buming mouth syndrome, postamputation pain, phantom pain, painful neuroma; traumatic neuroma, Morton’s neuroma, nerve entrapment injury, spinal stenosis, carpal tunnel syndrome, radicular pain, sciatica pain, nerve avulsion injury, brachial plexus avulsion injury, complex régional pain syndrome, drug therapy induced neuralgia, cancer chemotherapy induced neuralgia, anti-retroviral therapy induced neuralgia, post spinal cord injury pain, idiopathic small-fiber neuropathy, idiopathic sensory neuropathy or trigeminal autonomie neuropathy.

In yet another aspect, the invention provides the use of a compound or pharmaceutical composition of formula I for the manufacture of a médicament for use in treating or lessening the severity in a subject of musculoskeletal pain, wherein musculoskeletal pain comprises osteoarthritis pain, back pain, cold pain, bum pain or dental pain.

In yet another aspect, the invention the invention provides the use of a compound or pharmaceutical composition of formula I for the manufacture of a médicament for use in treating or lessening the severity in a subject of inflammatory pain, wherein inflammatory pain comprises rheumatoid arthritis pain or vulvodynia.

In yet another aspect, the invention the invention provides the use of a compound or pharmaceutical composition of formula I for the manufacture of a médicament for use in treating or lessening the severity in a subject of inflammatory pain, wherein inflammatory pain comprises rheumatoid arthritis pain.

In yet another aspect, the invention provides the use of a compound or pharmaceutical composition of formula I for the manufacture of a médicament for use in treating or lessening the severity in a subject of idiopathic pain, wherein idiopathic pain comprises fibromyalgia pain.

In yet another aspect, the invention provides the use of a compound or pharmaceutical composition of formula I for the manufacture of a médicament for use in treating or lessening the severity in a subject of pathological cough.

Tn yet another aspect, the invention provides the use of a compound or pharmaceutical composition of formula I for the manufacture of a médicament in combination with one or more additional therapeutic agents administered concurrently with, prior to, or subséquent to treatment with the compound or pharmaceutical composition.

Page 42 of 100

In another aspect, the invention provides the use of a compound or pharmaceutical composition of formula I for the manufacture of a médicament for use in treating or lessening the severity of acute pain, chronic pain, neuropathie pain, inflammatory pain, arthritis, migraine, cluster headaches, trigeminal neuralgia, herpetic neuralgia, general neuralgias, epilepsy, epilepsy conditions, neurodegenerative disorders, psychiatrie disorders, anxiety, dépréssion, dipolar disorder, myotonia, arrhythmia, movement disorders, neuroendocrine disorders, ataxia, multiple sclerosis, irritable bowel syndrome, incontinence, pathological cough, viscéral pain, osteoarthritis pain, postherpetic neuralgia, diabetic neuropathy, radicular pain, sciatica, back pain, head pain, neck pain, severe pain, intractable pain, nociceptive pain, breakthrough pain, postsurgical pain, cancer pain, stroke, cérébral ischemia, traumatic brain injury, amyotrophie latéral sclerosis, stress induced angina, exercise induced angina, palpitations, hypertension, or abnormal gastro-intestinal motility.

In another aspect, the invention provides the use of a compound or pharmaceutical composition of formula I for the manufacture of a médicament for use in treating or lessening the severity of fémur cancer pain, non-malignant chronic bone pain, rheumatoid arthritis, osteoarthritis, spinal stenosis, neuropathie low back pain, myofascial pain syndrome, fïbromyalgia, temporomandibular joint pain, chronic viscéral pain, abdominal pain, pancreatic pain, IBS pain, chronic and acute headache pain, migraine, tension headache, cluster headaches, chronic and acute neuropathie pain, post-herpetic neuralgia, diabetic neuropathy, HlV-associated neuropathy, trigeminal neuralgia, Charcot-Marie Tooth neuropathy, hereditary sensory neuropathy, peripheral nerve injury, painful neuromas, ectopic proximal and distal discharges, radiculopathy, chemotherapy induced neuropathie pain, radiotherapy-induced neuropathie pain, post-mastectomy pain, central pain, spinal cord injury pain, post-stroke pain, thalamic pain, complex régional pain syndrome, phantom pain, intractable pain, acute pain, acute post-operative pain, acute musculoskeletal pain, joint pain, mechanical low back pain, neck pain, tendonitis, injurypain, exercise pain, acute viscéral pain, pyelonephritis, appendicitis, cholecystitis, intestinal obstruction, hemias, chest pain, cardiac pain, pelvic pain, rénal colic pain, acute obstetric pain, labor pain, cesarean section pain, acute inflammatory pain, bum pain, trauma pain, acute intermittent pain, endometriosis, acute herpes zoster pain, sickle cell anémia, acute pancreatitis, breakthrough pain, orofacial pain, sinusitis pain, dental pain, multiple sclerosis (MS) pain, pain in dépréssion, leprosy pain, Behcet’s disease pain, adiposis dolorosa, phlebitic pain, Guillain-Barre pain, painful legs and moving toes, Haglund syndrome, erythromelalgia pain, Fabry’s disease pain, bladder and urogénital disease, urinary incontinence, pathological cough, hyperactive bladder, painful bladder syndrome, interstitial cyctitis (IC), prostatitis, complex régional pain syndrome (CRPS) type I complex régional pain syndrome (CRPS) type Π, widespread pain, paroxysmal extreme pain, pruritis, tinnitis, or angina-induced pain.

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In another aspect, the invention provides the use of a compound or pharmaceutical composition of formula I for the manufacture of a médicament for use in treating or lessening the severity of neuropathie pain. In one aspect, the neuropathie pain is selected from post-herpetic neuralgia, diabetic neuralgia, painful HIV-associated sensory neuropathy, trigeminal neuralgia, buming mouth syndrome, postamputation pain, phantom pain, painful neuroma, traumatic neuroma, Morton’s neuroma, nerve entrapment injury, spinal stenosis, carpal tunnel syndrome, radicular pain, sciatica pain, nerve avulsion injury, brachial plexus avulsion, complex régional pain syndrome, drug therapy induced neuralgia, cancer chemotherapy induced neuralgia, anti-retroviral therapy induced neuralgia, post spinal cord injury pain, idiopathic small-fïber neuropathy, idiopathic sensory neuropathy or trigeminal autonomie cephalalgia.

Administration of Pharmaceutically acceptable compositions.

In certain embodiments of the invention an “effective amount” of the compound, or pharmaceutically acceptable composition is that amount effective for treating or lessening the severity of one or more of chronic pain, gut pain, neuropathie pain, musculoskeletal pain, acute pain, inflammatory pain, cancer pain, idiopathic pain, multiple sclerosis, Charcot-Marie-Tooth syndrome, incontinence, pathological cough, or cardiac arrhythmia.

The compounds and compositions, according to the methods of the présent invention, may be administered using any amount and any route of administration effective for treating or lessening the severity of one or more of the pain or non-pain diseases recited herein. The exact amount required will vary from subject to subject, depending on the species, âge, and general condition of the subject, the severity of the infection, the particular agent, its mode of administration, and the like. The compounds of the invention are preferably formulated in dosage unit form for ease of administration and uniformity of dosage. The expression “dosage unit form” as used herein refers to a physically discrète unit of agent appropriate for the subject to be treated. It will be understood, however, that the total daily usage of the compounds and compositions of the invention will be decided by the attending physician within the scope of sound medical judgment. The spécifie effective dose level for any particular subject or organism will dépend upon a variety of factors including the disorder being treated and the severity of the disorder; the activity of the spécifie compound employed; the spécifie composition employed; the âge, body weight, general health, sex and diet of the subject; the time of administration, route of administration, and rate of excrétion of the spécifie compound employed; the duration of the treatment; drugs used in combination or coincidental with the spécifie compound employed, and like factors well known in the medical arts. The term “subject” or “patient,” as used herein, means an animal, preferably a mammal, and most preferably a human.

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The pharmaceutically acceptable compositions of this invention can be administered to humans and other animais orally, rectally, parenterally, intracistemally, intravaginally, intraperitoneally, topically (as by powders, ointments, or drops), bucally, as an oral or nasal spray, or the like, depending on the severity of the infection being treated. In certain embodiments, the compounds of the invention may be administered orally or parenterally at dosage levels of about 0.01 mg/kg to about 50 mg/kg and preferably from about 1 mg/kg to about 25 mg/kg, of subject body weight per day, one or more times a day, to obtain the desired therapeutic effect.

Liquid dosage forms for oral administration include, but are not limited to, pharmaceutically acceptable émulsions, microemulsions, solutions, suspensions, syrups and élixirs. In addition to the active compounds, the liquid dosage forms may contain inert diluents commonly used in the art such as, for example, water or other solvents, solubilizing agents and emulsifiers such as ethyl alcohol, isopropyl alcohol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1,3-butylène glycol, dimethylformamide, oils (in particular, cottonseed, groundnut, corn, germ, olive, castor, and sesame oils), glycerol, tetrahydrofurfuryl alcohol, polyethylene glycols and fatty acid esters of sorbitan, and mixtures thereof. Besides inert diluents, the oral compositions can also include adjuvants such as wetting agents, emulsifying and suspending agents, sweetening, flavoring, and perfuming agents.

Injectable préparations, for example, stérile injectable aqueous or oleaginous suspensions may be formulated according to the known art using suitable dispersing or wetting agents and suspending agents. The stérile injectable préparation may also be a stérile injectable solution, suspension or émulsion in a nontoxic parenterally acceptable diluent or solvent, for example, as a solution in 1,3-butanediol. Among the acceptable vehicles and solvents that may be employed are water, Ringer’s solution, U.S.P. and isotonie sodium chloride solution. In addition, stérile, fixed oils are conventionally employed as a solvent or suspending medium. For this purpose any bland fixed oil can be employed including synthetic mono- or diglycerides. In addition, fatty acids such as oleic acid are used in the préparation of injectables.

The injectable formulations can be sterilized, for example, by filtration through a bacterial-retaining fïlter, or by incorporating sterilizing agents in the form of stérile solid compositions which can be dissolved or dispersed in stérile water or other stérile injectable medium prior to use.

Tn order to prolong the effect of a compound of the invention, it is often désirable to slow the absorption of the compound from subeutaneous or intramuscular injection. This may be accomplished by the use of a liquid suspension of crystalline or amorphous material with poor water solubility. The rate of absorption of the compound then dépends upon its rate of dissolution that, in tum, may dépend upon crystal size and crystalline form. Alternatively, delayed absorption of a parenterally administered

Page 45 of 100 compound form is accomplished by dissolving or suspending the compound in an oil vehicle. Injectable depot forms are made by forming microencapsule matrices of the compound in biodégradable polymers such as polylactide-polyglycolide. Depending upon the ratio of compound to polymer and the nature of the particular polymer employed, the rate of compound release can be controlled. Examples of other biodégradable polymers include poly(orthoesters) and poly(anhydrides). Depot injectable formulations are also prepared by entrapping the compound in liposomes or microemulsions that are compatible with body tissues.

Compositions for rectal or vaginal administration are preferably suppositories which can be prepared by mixing the compounds of this invention with suitable non-irritating excipients or carriers such as cocoa butter, polyethylene glycol or a suppository wax which are solid at ambient température but liquid at body température and therefore melt in the rectum or vaginal cavity and release the active compound.

Solid dosage forms for oral administration include capsules, tablets, pills, powders, and granules. In such solid dosage forms, the active compound is mixed with at least one inert, pharmaceutically acceptable excipient or carrier such as sodium citrate or dicalcium phosphate and/or a) fïllers or extenders such as starches, lactose, sucrose, glucose, mannitol, and silicic acid, b) binders such as, for example, carboxymethylcellulose, alginates, gelatin, polyvinylpyrrolidinone, sucrose, and acacia, c) humectants such as glycerol, d) disintegrating agents such as agar—agar, calcium carbonate, potato or tapioca starch, alginic acid, certain silicates, and sodium carbonate, e) solution retarding agents such as paraffïn, f) absorption accelerators such as quatemary ammonium compounds, g) wetting agents such as, for example, cetyl alcohol and glycerol monostearate, h) absorbents such as kaolin and bentonite clay, and i) lubricants such as talc, calcium stéarate, magnésium stéarate, solid polyethylene glycols, sodium lauryl sulfate, and mixtures thereof. In the case of capsules, tablets and pills, the dosage form may also comprise buffering agents.

Solid compositions of a similar type may also be employed as fillers in soft and hard-filled gelatin capsules using such excipients as lactose or milk sugar as well as high molecular weight polyethylene glycols and the like. The solid dosage forms of tablets, dragees, capsules, pills, and granules can be prepared with coatings and shells such as enteric coatings and other coatings well known in the pharmaceutical formulating art. They may optionally contain opacifying agents and can also be of a composition that they release the active ingredient(s) only, or preferentially, in a certain part of the intestinal tract, optionally, in a delayed manner. Examples of embedding compositions that can be used include polymeric substances and waxes. Solid compositions of a similar type may also be employed as fillers in soft and hard-filled gelatin capsules using such excipients as lactose or milk sugar as well as high molecular weight polethylene glycols and the like.

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The active compounds can also be in microencapsulated form with one or more excipients as noted above. The solid dosage forms of tablets, dragees, capsules, pills, and granules can be prepared with coatings and shells such as enteric coatings, release controlling coatings and other coatings well known in the pharmaceutical formulating art. In such solid dosage forms the active compound may be admixed with at least one inert diluent such as sucrose, lactose or starch. Such dosage forms may also comprise, as is normal practice, additional substances other than inert diluents, e.g., tableting lubricants and other tableting aids such a magnésium stéarate and microcrystalline cellulose. In the case of capsules, tablets and pills, the dosage forms may also comprise buffering agents. They may optionally contain opacifying agents and can also be of a composition that they release the active ingredient(s) only, or preferentially, in a certain part of the intestinal tract, optionally, in a delayed manner. Examples of embedding compositions that can be used include polymeric substances and waxes.

Dosage forms for topical or transdermal administration of a compound of this invention include ointments, pastes, creams, lotions, gels, powders, solutions, sprays, inhalants or patches. The active component is admixed under stérile conditions with a pharmaceutically acceptable carrier and any needed preservatives or buffers as may be required. Ophthalmic formulation, eardrops, and eye drops are also contemplated as being within the scope of this invention. Additionally, the invention contemplâtes the use of transdermal patches, which hâve the added advantage of providing controlled delivery of a compound to the body. Such dosage forms are prepared by dissolving or dispensing the compound in the proper medium. Absorption enhancers can also be used to increase the flux of the compound across the skin. The rate can be controlled by either providing a rate controlling membrane or by dispersing the compound in a polymer matrix or gel.

As described generally above, the compounds of the invention are useful as inhibitors of voltage-gated sodium channels. In one embodiment, the compounds and compositions of the invention are inhibitors of Na_v1.8 and thus, without wishing to be bound by any particular theory, the compounds and compositions are particularly useful for treating or lessening the severity of a disease, condition, or disorder where activation or hyperactivity of Na_v1.8 is implicated in the disease, condition, or disorder. When activation or hyperactivity of Na_v1.8 is implicated in a particular disease, condition, or disorder, the disease, condition, or disorder may also be referred to as a “Na_v1.8 -mediated disease, condition or disorder.” Accordingly, in another aspect, the invention provides a method for treating or lessening the severity of a disease, condition, or disorder where activation or hyperactivity of Na_v1.8 is implicated in the disease state.

The activity of a compound utilized in this invention as an inhibitor of Na_v1.8 may be assayed according to methods described generally in the Examples herein, or according to methods available to one of

Page 47 oflOO ordinary skill in the art.

Additional Therapeutic Agents

It will also be appreciated that the compounds and pharmaceutically acceptable compositions of the invention can be employed in combination thérapies, that is, the compounds and pharmaceutically acceptable compositions can be administered concurrently with, prior to, or subséquent to, one or more other desired therapeutics or medical procedures. The particular combination of thérapies (therapeutics or procedures) to employ in a combination regimen will take into account compatibility of the desired therapeutics and/or procedures and the desired therapeutic effect to be achieved. It will also be appreciated that the thérapies employed may achieve a desired effect for the same disorder (for example, an inventive compound may be administered concurrently with another agent used to treat the same disorder), or they may achieve different effects (e.g., control of any adverse effects). As used herein, additional therapeutic agents that are normally administered to treat or prevent a particular disease, or condition, are known as “appropriate for the disease, or condition, being treated.” For example, exemplary additional therapeutic agents include, but are not limited to: nonopioid analgésies (indoles such as Etodolac, Indomethacin, Sulindac, Tolmetin; naphthylalkanones such sa Nabumetone; oxicams such as Piroxicam; para-aminophenol dérivatives, such as Acetaminophen; propionic acids such as Fenoprofen, Flurbiprofen, Ibuprofen, Ketoprofen, Naproxen, Naproxen sodium, Oxaprozin; salicylates such as Asprin, Choline magnésium trisalicylate, Diflunisal; fenamates such as meelofenamie acid, Mefenamic acid; and pyrazoles such as Phenylbutazone); or opioid (narcotic) agonists (such as Codeine, Fentanyl, Hydromorphone, Levorphanol, Meperidine, Methadone, Morphine, Oxycodone, Oxymorphone, Propoxyphene, Buprénorphine, Butorphanol, Dezocine, Nalbuphine, and Pentazocine). Additionally, nondrug analgésie approaches may be utilized in conjunction with administration of one or more compounds of the invention. For example, anesthesiologic (intraspinal infusion, neural blocade), neurosurgical (neurolysis of CNS pathways), neurostimulatory (transcutaneous electrical nerve stimulation, dorsal column stimulation), physiatric (physical therapy, orthotic devices, diathermy), or psychologie (cognitive methods-hypnosis, biofeedback, or behavioral methods) approaches may also be utilized. Additional appropriate therapeutic agents or approaches are described generally in The Merck Manual, Nineteenth Edition, Ed. Robert S. Porter and Justin L. Kaplan, Merck Sharp & Dohme Corp., a subsidiary of Merck & Co., Inc., 2011, and the Food and Drug Administration website, www.fda.gov, the entire contents of which are hereby incorporated by reference.

In another embodiment, additional appropriate therapeutic agents are selected from the following:

(1) an opioid analgésie, e.g. morphine, heroin, hydromorphone, oxymorphone, levorphanol, levallorphan, methadone, meperidine, fentanyl, cocaïne, codeine, dihydrocodéine, oxycodone, hydrocodone,

Page 48 of 100 propoxyphene, nalmefene, nalorphine, naloxone, naltrexone, buprénorphine, butorphanol, nalbuphine or pentazocine;

(2) a nonsteroidal antiinflaminatory drug (NSAID), e.g. aspirin, diclofenac, diflusinal, etodolac, fenbufen, fenoprofen, flufenisal, flurbiprofen, ibuprofen, indomethacin, ketoprofen, ketorolac, meclofenamic acid, mefenamic acid, meloxicam, nabumetone, naproxen, nimesulide, nitroflurbiprofen, olsalazine, oxaprozin, phenylbutazone, piroxicam, sulfasalazine, sulindac, tolmetin or zomepirac;

(3) a barbiturate sédative, e.g. amobarbital, aprobarbital, butabarbital, butabital, mephobarbital, metharbital, methohexital, pentobarbital, phenobartital, secobarbital, talbutal, theamylal or thiopental;

(4) a benzodiazépine having a sédative action, e.g. chlordiazepoxide, clorazepate, diazepam, flurazepam, lorazépam, oxazepam, temazepam or triazolam;

(5) a histamine (Hi) antagonist having a sédative action, e.g. diphenhydramine, pyrilamine, promethazine, chloipheniramine or chlorcyclizine;

(6) a sédative such as glutethimide, meprobamate, methaqualone or dichloralphenazone;

(7) a skeletal muscle relaxant, e.g. baclofen, carisoprodol, chlorzoxazone, cyclobenzaprine, methocarbamol or orphrenadine;

(8) an NMDA receptor antagonist, e.g. dextromethorphan ((+)-3-hydroxy-N- methylmorphinan) or its métabolite dextrorphan ((+)-3-hydroxy-N-methylmorphinan), ketamine, memantine, pyrroloquinoline quinine, cis-4-(phosphonomethyl)-2-piperidinecarboxylic acid, budipine, EN-3231 (MorphiDex®), a combination formulation of morphine and dextromethorphan), topiramate, neramexane or perzinfotel including anNR2B antagonist, e.g. ifenprodil, traxoprodil or (-)-(R)-6-{2-[4-(3-fluorophenyl)-4-hydroxy1- piperidinyI]-l-hydroxyethyl-3,4-dihydro-2(lH)-quinolinone;

(9) an alpha-adrenergic, e.g. doxazosin, tamsulosin, clonidine, guanfacine, dexmetatomidine, modafinil, or 4-amino-6,7-dimethoxy-2-(5-methane-sulfonamido-l, 2,3,4- tetrahydroisoquinol-2-yl)-5-(2-pyridyl) quinazoline;

(10) a tricyclic antidepressant, e.g. desipramine, imipramine, amitriptyline or nortriptyline;

(11) an anticonvulsant, e.g. carbamazepine (Tegretol®), lamotrigine, topiramate, lacosamide (Vimpat®) or valproate;

(12) a tachykinin (NK) antagonist, particularly an NK-3, NK-2 or NK-1 antagonist, e.g. (alphaR,9R)-7[3,5-bis(trifluoromethyl)benzyl]-8,9,10,l 1 -tetrahydro-9-methyl-5-(4- methylphenyl)-7H[l,4]diazocino[2,l-g][l,7]-naphthyridine-6-13-dione (TAK-637), 5- [[(2R,3S)-2-[(lR)-l-[3,5bis(trifluoromethyl)phenyl]ethoxy-3-(4-fluorophenyl)-4-morpholinyl]-methyl]-l,2-dihydro-3H-l,2,4

Page 49 of 100 triazol-3-one (MK-869), aprepitant, lanepitant, dapitant or 3-[[2-methoxy-5-(trifluoromethoxy)phenyl]methylamino]-2-phenylpiperidine (2S,3 S);

(13) a muscarinic antagonist, e.g oxybutynin, tolterodine, propiverine, tropsium chloride, darifenacin, solifenacin, temiverine and ipratropium;

(14) a COX-2 sélective inhibitor, e.g. celecoxib, rofecoxib, parecoxib, valdecoxib, deracoxib, etoricoxib, or lumiracoxib;

(15) a coal-tar analgésie, in particular paracétamol;

(16) a neuroleptic such as droperidol, chlorpromazine, haloperidol, perphenazine, thioridazine, mesoridazine, trifluoperazine, fluphenazine, clozapine, olanzapine, rispéridone, ziprasidone, quetiapine, sertindole, aripiprazole, sonepiprazole, blonanserin, iloperidone, perospirone, raclopride, zotepine, bifeprunox, asenapine, lurasidone, amisulpride, balaperidone, palindore, eplivanserin, osanetant, rimonabant, meclinertant, Miraxion® or sarizotan;

(17) a vanilloid receptor agonist (e.g. resinferatoxin or civamide) or antagonist (e.g. capsazepine, GRC15300);

(18) a beta-adrenergic such as propranolol;

(19) a local anaesthetic such as mexiletine;

(20) a corticosteroid such as dexamethasone;

(21) a 5-HT receptor agonist or antagonist, particularly a 5-HTi_B/id agonist such as eletriptan, sumatriptan, naratriptan, zolmitriptan or rizatriptan;

(22) a 5-HT₂a receptor antagonist such as R(+)-alpha-(2,3-dimethoxy-phenyl)-l-[2-(4fluorophenylethyl)]-4-piperidinemethanol (MDL-100907);

(23) a cholinergic (nicotinic) analgésie, such as ispronicline (TC-1734), (E)-N-methyl-4-(3-pyridinyl)-3buten-l-amine (RJR-2403), (R)-5-(2-azetidinylmethoxy)-2-chloropyridine (ABT-594) or nicotine;

(24) Tramadol®, Tramadol ER (Ultram ER®), Tapentadol ER (Nucynta®);

(25) a PDE5 inhibitor, such as 5-[2-ethoxy-5-(4-methyl-l-piperazinyl-sulphonyl)phenyl]-l-methyl-3-npropyl-l,6-dihydro-7H-pyrazolo[4,3-d]pyrimidin-7-one (sildenafïl), (6R,12aR)- 2,3,6,7,12,12ahexahydro-2-methyl-6-(3,4-methylenedioxyphenyl)-pyrazino[2',r:6,l]-pyrido[3,4-b]indole-l,4-dione (IC-

351 or tadalafîl), 2-[2-ethoxy-5-(4-ethyl-piperazin-l-yl-l-sulphonyl)-phenyl]-5-methyl-7-propyl-3Himidazo[5,l-f][l,2,4]triazin-4-one (vardenafil), 5-(5-acetyl-2-butoxy-3-pyridinyl)-3-ethyl-2-(l-ethyl-3azetidinyl)-2,6-dihydro-7Æ-pyrazolo[4,3-i/]pyrimidin-7-one, 5-(5-acetyl-2-propoxy-3-pyridinyl)-3-ethyl

Page 50 of 100

2-(l-isopropyl-3-azetidinyl)-2,6-dihydro-777-pyrazolo[4,3-rZ]pvrimidin-7-one, 5-[2-ethoxy-5-(4ethylpiperazin-l-ylsulphonyl)pyridin-3-yl]-3-ethyl-2-[2-methoxyethyl]-2,6-dihydro-7H-pyrazolo[4,3d]pyrimidin-7-one, 4-[(3-chloro-4-methoxybenzyl)amino]-2-[(2S)-2-(hydroxymethyl)pyrrolidin-l-yl]-N(pyrimidin-2-ylmethyl)pyriniidine-5-carboxaniide, 3-(l-methyl-7-oxo-3-propyl-6,7-dihydro-lHpyrazolo[4,3-d]pyrimidin-5-yl)-N-[2-(l-methylpyrrolidin-2-yl)ethyl]-4-propoxybenzenesulfonamide;

(26) an alpha-2-delta ligand such as gabapentin (Neurontin®), gabapentin GR (Gralise®), gabapentin, enacarbil (Horizant®), pregabalin (Lyrica®), 3-methyl gabapentin, (l[alpha],3[alpha],5[alpha])(3-aminomethyl-bicyclo[3.2.0]hept-3-yl)-acetic acid, (3S,5R)-3-aminomethyl-5-methyl-heptanoic acid, (3S,5R)-3amino-5-methyl-heptanoic acid, (3S,5R)-3-amino-5-methyl-octanoic acid, (2S,4S)-4-(3chlorophenoxy)proline, (2S,4S)-4-(3-fluorobenzyl)-proline, [(lR,5R,6S)-6(aminomethyl)bicyclo[3.2.0]hept-6-yl]acetic acid, 3-(l-aminomethyl-cyclohexylmethyl)-4H[l,2,4]oxadiazol-5-one, C-[l-(lH-tetrazol-5-ylmethyl)-cycloheptyl]-methylamine, (3S,4S)-(laminomethyl-3,4-dimethyl-cyclopentyl)-acetic acid, (3S,5R)-3-aminomethyl-5-methyl-octanoic acid, (3S,5R)-3-amino-5-methyl-nonanoic acid, (3S,5R)-3-amino-5-methyl-octanoic acid, (3R,4R,5R)-3amino-4,5-dimethyl-heptanoic acid and (3R,4R,5R)-3-amino-4,5-dimethyl-octanoic acid;

(27) a cannabinoid such as KHK-6188;

(28) metabotropic glutamate subtype 1 receptor (mGluRl) antagonist;

(29) a serotonin reuptake inhibitor such as sertraline, sertraline métabolite demethylsertraline, fluoxetine, norfluoxetine (fluoxetine desmethyl métabolite), fluvoxamine, paroxetine, citalopram, citalopram métabolite desmethylcitalopram, escitalopram, d,l-fenfluramine, femoxetine, ifoxetine, cyanodothiepin, litoxetine, dapoxetine, nefazodone, cericlamine and trazodone;

(30) a noradrenaline (norepinephrine) reuptake inhibitor, such as maprotiline, lofepramine, mirtazepine, oxaprotiline, fezolamine, tomoxetine, mianserin, buproprion, buproprion métabolite hydroxybuproprion, nomifensine and viloxazine (Vivalan®), especially a sélective noradrenaline reuptake inhibitor such as reboxetine, in particular (S,S)-reboxetine;

(31) a dual serotonin-noradrenaline reuptake inhibitor, such as venlafaxine, venlafaxine métabolite Odesmethylvenlafaxine, clomipramine, clomipramine métabolite desmethylclomipramine, duloxetine (Cymbalta®), milnacipran and imipramine;

(32) an inducible nitric oxide synthase (iNOS) inhibitor such as S-[2-[(l-iminoethyl)amino]ethyl]-Lhomocysteine, S-[2-[(l-iminoethyl)-amino]ethyl]-4,4-dioxo-L-cysteine, S-[2-[(l-iminoethyI)amino]ethyI]2-methyl-L-cysteine, (2S,5Z)-2-amino-2-methyl-7-[(l-iminoethyl)amino]-5-heptenoic acid, 2-[[(lR,3S)-3amino-4-hydroxy-l-(5-thiazolyl)-butyl]thio]-S-chloro-S-pyridinecarbonitrile; 2-[[(lR,3S)-3-amino-4Page 51 of 100 hydroxy-l-(5- thiazolyl)butyl]thio]-4-chlorobenzonitrile, (2S,4R)-2-amino-4-[[2-chloro-5(trifluoromethyl)phenyl]thio]-5-thiazolebutanol, 2-[[(lR,3S)-3-amino-4-hydroxy-l-(5-thiazolyl) butyl]thio]-6-(trifluoromethyl)-3-pyridinecarbonitrile, 2-[[(lR,3S)-3-amino-4-hydroxy-l-(5thiazolyl)butyl]thio]-5-chlorobenzonitrile, N-[4-[2-(3-chlorobenzylamino)ethyl]phenyl]thiophene-2carboxamidine, NXN-462, or guanidinoethyldisulfïde;

(33) an acetylcholinesterase inhibitor such as donepezil;

(34) a prostaglandin E2 subtype 4 (EP4) antagonist such as 7V-[({2-[4-(2-ethyl-4,6- dimethyl-lHimidazo[4,5-c]pyridin-l-yl)phenyl]ethyl}amino)-carbonyl]-4- methylbenzenesulfonamide or 4-[( 15)-1({[5-chloro-2-(3-fluorophenoxy)pyridin-3-yl]carbonyl}amino)ethyl]benzoic acid;

(35) a leukotriene B4 antagonist; such as l-(3-biphenyl-4-ylmethyl-4-hydroxy-chroman-7-yl)cyclopentanecarboxylic acid (CP- 105696), 5-[2-(2-Carboxyethyl)-3-[6-(4-methoxyphenyl)-5Ehexenyl]oxyphenoxy]-valeric acid (ONO-4057) or DPC-11870;

(36) a 5-lipoxygenase inhibitor, such as zileuton, 6-[(3-fluoro-5-[4-methoxy-3,4,5,6- tetrahydro-2Hpyran-4-yl])phenoxy-methyl]-l-methyl-2-quinolone (ZD-2138), or 2,3,5- trimethyl-6-(3-pyridylmethyl)-

1,4-benzoquinone (CV-6504);

(37) a sodium channel blocker, such as lidocaine, lidocaine plus tetracaine cream (ZRS-201) or eslicarbazepine acetate;

(38) an Na_v1.7 blocker, such as XEN-402, XEN403, TV-45070, PF-05089771, CNV1014802, GDC0276, RG7893 and such as those disclosed in WO2011/140425; WO2012/106499; WO2012/112743; WO2012/125613, WO2012/116440, WO2011026240, US8883840, US8466188, or PCT/US2013/21535 the entire contents of each application hereby incorporated by reference.

(38a) anNa_v1.7 blocker such as (2-benzylspiro[3,4-dihydropyrrolo[l,2-a]pyrazine-l,4'-piperidine]-r-yl)(4-isopropoxy-3-methyl-phenyl)methanone, 2,2,2-trifluoro-l-[l'-[3-methoxy-4-[2(trifluoromethoxy)ethoxy]benzoyl]-2,4-dimethyl-spiro[3,4-dihydropyrrolo[l,2-a]pyrazine-l,4'piperidine]-6-yl]ethanone, [8-fluoro-2-methyl-6-(trifluoromethyl)spiro[3,4-dihydropyrrolo[l,2a]pyrazine-l,4'-piperidine]-l'-yl]-(4-isobutoxy-3-methoxy-phenyl)methanone, l-(4-benzhydrylpiperazin1 -yl)-3-[2-(3,4-dimethylphenoxy)ethoxy]propan-2-ol, (4-butoxy-3-methoxy-phenyl)-[2-methyl-6(trifluoromethyl)spiro[3,4-dihydropyrrolo[l,2-a]pyrazine-l,4'-piperidine]-l'-yljmethanone, [8-fluoro-2methyl-6-(trifluoromethyl)spiro[3,4-dihydropyrrolo[l,2-a]pyrazine-l,4'-piperidine]-r-yl]-(5-isopropoxy6-methyl-2-pyridyl)methanone, (4-isopropoxy-3-methyl-phenyl)-[2-methyl-6-(l, 1,2,2,2pentafluoroethyl)spiro[3,4-dihydropyrrolo[l,2-a]pyrazine-l,4'-piperidine]-l'-yl]methanone, 5-[2-methyl4-[2-methyl-6-(2,2,2-trifluoroacetyl)spiro[3,4-dihydropyrrolo[l,2-a]pyrazine-l,4^,-piperidine]-l·

Page 52 of 100 carbonyl]phenyl]pyridine-2-carbonitrile, (4-isopropoxy-3-methyl-phenyl)-[6-(trifluoromethyl)spiro[3,4dihydro-2H-pyrrolo[l,2-a]pyrazine-l,4'-piperidine]-l'-yl]niethanone, 2,2,2-trifluoro-l-[l'-[3-methoxy-4[2-(trifluoromethoxy)ethoxy]benzoyl]-2-niethyl-spiro[3,4-dihydropyrrolo[l,2-a]pyrazine-l,4'piperidine]-6-yl]ethanone, 2,2,2-trifluoro-l-[r-(5-isopropoxy-6-methyl-pyridine-2-carbonyl)-3,3dimethyl-spiro[2,4-dihydropyrrolo[l,2-a]pyrazine-l,4'-piperidine]-6-yl]ethanone, 2,2,2-trifluoro-l-[l'-(5isopentyloxypyridine-2-carbonyl)-2-methyl-spiro[3,4-dihydropyrrolo[l,2-a]pyrazine-l,4'-piperidine]-6yljethanone, (4-isopropoxy-3-mefhoxy-phenyl)-[2-methyl-6-(trifluoromethyl)spiro[3,4dihydropyrrolo[l,2-a]pyrazine-l,4'-piperidine]-r-yl]methanone, 2,2,2-trifluoro-l-[l'-(5isopentyIoxypyridine-2-carbonyl)-2,4-dimethyl-spiro[3,4-dihydropyrrolo[l,2-a]pyrazine-l,4'-piperidine]6-yl]ethanone, l-[(3S)-2,3-dimethyl-r-[4-(3,3,3-trifluoropropoxymethyl)benzoyl]spnO[3,4dihydropyrrolotljZ-alpyrazine-l^'-piperidineJ-ô-ylJ^Z^-trifluoro-ethanone, [8-fluoro-2-methyl-6(trifluoromethyl)spiro[3,4-dihydropyrrolo[l,2-a]pyrazine-l,4'-piperidine]-r-yl]-[3-methoxy-4-[(lR)-lmethylpropoxy]phenyl]methanone, 2,2,2-trifluoro-l-[l'-(5-isopropoxy-6-methyl-pyridine-2-carbonyl)-

2,4-dimethyl-spiro[3,4-dihydropyrrolo[l,2-a]pyrazine-l,4'-piperidine]-6-yl]etlianone, l-[l'-[4-methoxy-3(trifluoromethyl)benzoyl]-2-methyl-spiro[3,4-dihydropyrrolo[l,2-a]pyrazine-l,4'-piperidine]-6-yl]-2,2dimethyl-propan-l-one, (4-isopropoxy-3-methyl-phenyl)-[2-niethyl-6-(trifluoromethyl)spiro[3,4dihydropyrrolofl^-ajpyrazine-l^'-piperidinej-l'-yljmethanone, [2-methyl-6-(lmethyIcycIopropanecarbonyl)spiro[3,4-dihydropyrrolo[l,2-a]pyrazine-l,4'-pip^eridine]-l'-yl]-[4-(3,3,3trifluoropropoxymethyl)phenyl]methanone, 4-bromo-N-(4-bromophenyl)-3-[(l-methyl-2-oxo-4piperidyl)sulfamoyl]benzamide or (3-chloro-4-isopropoxy-pbenyl)-[2-methyl-6-(l, 1,2,2,2pentafluoroethyl)spiro[3,4-dihydropyrrolo[l,2-a]pyrazine-l,4'-piperidine]-r-yl]methanone.

(39) an Na_v1.8 blocker, such as PF-04531083, PF-06372865 and such as those disclosed in WO2008/135826, W02006/011050, W02013/061205, US20130303535, W02013131018, US8466188, WO2013114250, W02014/1280808, W02014/120815 and WO2014/120820, the entire contents of each application hereby incorporated by reference.

(39a) anNa_v1.8 blocker such as 4,5-dichloro-2-(4-fluoro-2-methoxyphenoxy)-N-(2-oxo-l,2dihydropyridin-4-yl)benzamide, 2-(4-fluoro-2-methoxyphenoxy)-N-(2-oxo-l,2-dihydropyridin-4-yl)-4(perfluoroethyl)benzamide, 4,5-dichloro-2-(4-fluorophenoxy)-N-(2-oxo-l,2-dihydropyridin-4yl)benzamide, 4,5-dichloro-2-(3-fluoro-4-methoxyphenoxy)-N-(2-oxo-l,2-dihydropyridin-4yl)benzamide, 2-(4-fluoro-2-methoxyphenoxy)-N-(2-oxo-1,2-dihydropyridin-4-yl)-5(trifluoromethyl)benzamide, N-(2-oxo-l,2-dihydropyridin-4-yl)-2-(4-(trifluoromethoxy)phenoxy)-4(trifluoromethyl)benzamide, 2-(4-fluorophenoxy)-N-(2-oxo-l,2-dihydropyridin-4-yl)-4(perfluoroethyl)benzamide, 5-chloro-2-(4-fluoro-2-methoxyphenoxy)-N-(2-oxo-l,2-dihydropyridin-4yl)benzamide, N-(2-oxo-l,2-dihydropyridin-4-yl)-2-(4-(trifluoromethoxy)phenoxy)-5

Page 53 of 100 (trifluoromethyl)benzamide, 2-(4-fhioro-2-methylphenoxy)-N-(2-oxo-l,2-dihydropyridin-4-yl)-5(trifluoromethyl)benzamide, 2-(2-chloro-4-fluorophenoxy)-N-(2-oxo-l,2-dihydropyridin-4-yl)-5(trifhioromethyl)benzamide, 5-chloro-2-(4-fluoro-2-methylphenoxy)-N-(2-oxo-l,2-dihydropyridin-4yl)benzamide, 4-chloro-2-(4-fluoro-2-methylphenoxy)-N-(2-oxo-l ,2-dihydropyridin-4-yl)benzamide, 5chloro-2-(2-chloro-4-fluorophenoxy)-N-(2-oxo-l,2-dihydropyridin-4-yl)benzamide, 2-((5-fluoro-2hydroxybenzyl)oxy)-N-(2-oxo-l ,2-dihydropyridin-4-yl)-4-(trifluoromethyl)benzamide, N-(2-oxo-l ,2dihydropyridin-4-yl)-2-(o-tolyloxy)-5-(trifluoromethyl)benzamide, 2-(2,4-difluorophenoxy)-N-(2-oxo-

1,2-dihydropyridin-4-yl)-4-(trifluoromethyl)benzamide, N-(2-oxo-l ,2-dihydropyridin-4-yl)-2-(2(trifluoromethoxy)phenoxy)-5-(trifluoromethyl)benzamide, 2-(4-fluorophenoxy)-N-(2-oxo-l,2dihydropyridin-4-yl)-5-(trifluoromethyl)benzamide, In one embodiment, the compound is 3-(4-fluoro-2methoxyphenoxy)-N-(3 -(methylsulfonyl)phenyl)quinoxaline-2-carboxamide, 3 -(2-chloro-4fluorophenoxy)-N-(3-sulfamoylphenyl)quinoxaline-2-carboxamide, 3-(2-chloro-4-methoxyphenoxy)-N(3-sulfamoylphenyl)quinoxaline-2-carboxamide, 3-(4-chloro-2-methoxyphenoxy)-N-(3sulfamoylphenyl)quinoxaline-2-carboxamide, 4-(3-(4-(trifluoromethoxy)phenoxy)quinoxaline-2carboxamido)picolinic acid, 2-(2,4-difluorophenoxy)-N-(3-sulfamoylphenyl)quinoline-3-carboxamide, 2(4-fluoro-2-methoxyphenoxy)-N-(3 -sulfamoylphenyl)quinoline-3 -carboxamide, 3 -(2,4-difluorophenoxy)N-(3-sulfamoylphenyl)quinoxaline-2-carboxamide, N-(3-sulfamoylphenyl)-2-(4(trifluoromethoxy)phenoxy)quinoline-3-carboxamide, N-(3-sulfamoylphenyl)-3-(4(trifluoromethoxy)phenoxy)quinoxaline-2-carboxamide, 3-(4-chloro-2-methylphenoxy)-N-(3sulfamoylphenyl)quinoxaline-2-carboxamide, 5-(3-(4-(trifluoromethoxy)phenoxy)quinoxaline-2carboxamido)picolinic acid, 3-(4-fluoro-2-methoxyphenoxy)-N-(2-oxo-2,3-dihydro-lHbenzo[d]imidazol-5-yl)quinoxaline-2-carboxamide, 3-(4-fluoro-2-methoxyphenoxy)-N-(pyridin-4yl)quinoxaline-2-carboxamide, 3-(4-fluorophenoxy)-N-(3-sulfamoylphenyl)quinoxaline-2-carboxamide, N-(3-cyanophenyl)-3-(4-fluoro-2-methoxyphenoxy)quinoxaline-2-carboxamide, N-(4carbamoylphenyl)-3-(4-fluoro-2-methoxyphenoxy)quinoxaline-2-carboxamide, 4-(3-(4(trifluoromethoxy)phenoxy)quinoxaline-2-carboxamido)benzoic acid, N-(4-cyanophenyl)-3-(4-fluoro-2methoxyphenoxy)quinoxaline-2-carboxamide, 5-(4,5-dichloro-2-(4-fluoro-2methoxyphenoxy)benzamido)picolinic acid, 5-(2-(2,4-dimethoxyphenoxy)-4,6bis(trifluoromethyl)benzamido)picolmic acid, 4-(4,5-dichloro-2-(4-fluoro-2methoxyphenoxy)benzamido)benzoic acid, 5-(2-(4-fluoro-2-methoxyphenoxy)-4,6bis(trifluoromethyl)benzamido)picolinic acid, 4-(2-(4-fluoro-2-methoxyphenoxy)-4(perfluoroethyl)benzamido)benzoic acid, 5-(2-(4-fluoro-2-methoxyphenoxy)-4(perfluoroethyl)benzamido)picolinic acid, 4-(2-(4-fluoro-2-methylphenoxy)-4(trifluoromethyl)benzamido)benzoic acid, 5-(4,5-dichloro-2-(4-fluoro-2

Page 54 of 100 methoxyphenoxy)benzamido)picolinic acid, 4-(2-(2-chloro-4-fluorophenoxy)-4(perfluoroethyl)benzamido)benzoic acid, 4-(2-(4-fluoro-2-methylphenoxy)-4(perfluoroethyl)benzamido)benzoic acid, 4-(4,5-dichloro-2-(4(trifluoromethoxy)phenoxy)benzamido)benzoic acid, 4-(4,5-dichloro-2-(4-chloro-2methylphenoxy)benzamido)benzoic acid, 5-(4-(tert-butyl)-2-(4-fluoro-2methoxyphenoxy)benzamido)picolinic acid, 5-(4,5-dichloro-2-(4(frifluoromethoxy)phenoxy)benzamido)picolinic acid, 4-(4,5-dichloro-2-(4-fluoro-2methylphenoxy)benzamido)benzoic acid, 5-(4,5-dichloro-2-(2,4-dimethoxyphenoxy)benzamido)picolinic acid, 5-(4,5-dichloro-2-(2-chloro-4-fluorophenoxy)benzamido)picolinic acid, 5-(4,5-dichloro-2-(4-fluoro2-methylphenoxy)benzamido)picolinic acid, 4-(4,5-dichloro-2-(4-chloro-2methoxyphenoxy)benzamido)benzoic acid, 5-(4,5-dichloro-2-(2,4-difluorophenoxy)benzamido)picolinic acid, 2-(4-fluorophenoxy)-N-(3 -sulfamoylphenyl)-5-(trifluoromethyl)benzamide, 2-(4-fluorophenoxy)-N(3-sulfamoyIphenyl)-4-(trifluoromethyl)benzamide, 2-(2-chloro-4-fluorophenoxy)-N-(3sulfamoylphenyl)-5-(trifluoromethyl)benzamide, 2-(4-fluorophenoxy)-N-(3-sulfamoylphenyl)-4(trifluoromethyl)benzamide, 2-(2-chloro-4-fluorophenoxy)-N-(3-sulfamoylphenyl)-6(trifluoromethyl)benzamide, 2-(2-chloro-4-fluorophenoxy)-5-(difluoromethyl)-N-(3sulfamoylphenyl)benzamide, 2-(4-fluorophenoxy)-4-(perfluoroethyl)-N-(3-sulfamoylphenyl)benzamide, 2-(4-chloro-2-methoxyphenoxy)-4-(perfluoroethyl)-N-(3-sulfamoylphenyl)benzamide, 2-(4-fluoro-2methoxyphenoxy)-N-(3-sulfamoylphenyl)-5-(trifluoromethyl)benzamide, 5-chloro-2-(4-fluoro-2methylphenoxy)-N-(3-sulfamoylphenyl)benzamide, 4,5-dichloro-2-(4-fluoro-2-methoxyphenoxy)-N-(3sulfamoylphenyl)benzamide, 2,4-dichloro-6-(4-chloro-2-methoxyphenoxy)-N-(3sulfamoylphenyl)benzamide, 2,4-dichloro-6-(4-fluoro-2-methylphenoxy)-N-(3sulfamoylphenyl)benzamide, 2-(4-fluoro-2-methoxyphenoxy)-N-(3-sulfamoylphenyl)-4,6bis(trifluoromethyl)benzamide, 2-(4-fluoro-2-methylphenoxy)-N-(3-sulfamoylphenyl)-4,6bis(trifluoromethyl)benzamide, 5-chloro-2-(2-chloro-4-fluorophenoxy)-N-(3sulfamoylpbenyl)benzamide, 2-(4-fluoro-2-methoxyphenoxy)-N-(3-sulfamoylphenyl)-4(trifluoromethoxy)benzamide, 2-(4-fluoro-2-methoxyphenoxy)-N-(3-sulfamoylphenyl)-4(trifluoromethyl)benzamide, 4,5-dichloro-2-(4-fluorophenoxy)-N-(3-sulfamoylphenyl)benzainide, 2-(4fluoro-2-methoxyphenoxy)-4-(perfluoroethyl)-N-(3-sulfamoylphenyl)benzamide, 5-fluoro-2-(4-fluoro-2metbylpbenoxy)-N-(3 -sulfamoylphenyl)benzamide, 2-(2-chloro-4-fluorophenoxy)-4-cyano-N-(3 sulfamoylphenyl)benzamide or N-(3-sulfamoylphenyl)-2-(4-(trifluoromethoxy)phenoxy)-4(trifluoromethyl)benzaniide.

(40) a combined Na_v1.7 and Na_v1.8 blocker, such as DSP-2230 or BL-1021;

(41) a 5-HT3 antagonist, such as ondansetron;

Page 55 oflOO (42) a TPRV 1 receptor agonist, such as capsaicin (NeurogesX®, Qutenza®); and the pharmaceutically acceptable salts and solvatés thereof;

(43) a nicotinic receptor antagonist, such as varenicline;

(44) an N-type calcium channel antagonist, such as Z-160;

(45) a nerve growth factor antagonist, such as tanezumab;

(46) an endopeptidase stimulant, such as senrebotase;

(47) an angiotensin Π antagonist, such as EMA-401;

In one embodiment, the additional appropriate therapeutic agents are selected from V-l 16517, Pregbalin, controlled release Pregbalin, Ezogabine (Potiga®), Ketamine/amitriptyline topical cream (Amiket®), AVP-923, Perampanel (E-2007), Ralfinamide, transdermal bupivacaine (Eladur®), CNV1014802, JNJ10234094 (Carisbamate), BMS-954561 or ARC-4558.

In another embodiment, the additional appropriate therapeutic agents are selected from N-(6-amino-5(2,3,5-trichlorophenyl)pyridin-2-yl)acetamide; N-(6-amino-5-(2-chloro-5-methoxyphenyl)pyridin-2-yl)l-methyl-lH-pyrazole-5-carboxamide; or 3-((4-(4-(trifluoromethoxy)phenyl)-lH-imidazol-2yl)methyl)oxetan-3 -amine.

The amount of additional therapeutic agent présent in the compositions of this invention will be no more than the amount that would normally be administered in a composition comprising that therapeutic agent as the only active agent. The amount of additional therapeutic agent in the presently disclosed compositions will range from about 10% to 100% of the amount normally présent in a composition comprising that agent as the only therapeutically active agent.

The compounds of this invention or pharmaceutically acceptable compositions thereof may also be incorporated into compositions for coating an implantable medical device, such as prostheses, artificial valves, vascular grafts, stents and cathéters. Accordingly, the invention, in another aspect, includes a composition for coating an implantable device comprising a compound of the invention as described generally above, and in classes and subclasses herein, and a carrier suitable for coating said implantable device. In still another aspect, the invention includes an implantable device coated with a composition comprising a compound of the invention as described generally above, and in classes and subclasses herein, and a carrier suitable for coating said implantable device. Suitable coatings and the general préparation of coated implantable devices are described in US Patents 6,099,562; 5,886,026; and 5,304,121. The coatings are typically biocompatible polymeric materials such as a hydrogel polymer, polymethyldisiloxane, polycaprolactone, polyethylene glycol, polylactic acid, ethylene vinyl acetate, and mixtures thereof. The coatings may optionally be further covered by a suitable topcoat of fluorosilicone,

Page 56 of 100 polysaccarides, polyethylene glycol, phospholipids or combinations thereof to impart controlled release characteristics in the composition.

Another aspect of the invention relates to inhibiting Na_v1.8 activity in a subject, which method comprises administering to the subject a compound of formula I or a composition comprising said compound. Yet another aspect of the invention relates to inhibiting Na_v1.8 activity in a biological sample, which method comprises contacting said biological sample with a compound of formula I or a composition comprising said compound. The term “biological sample,” as used herein, includes, without limitation, cell cultures or extracts thereof; biopsied material obtained from a mammal or extracts thereof; and blood, saliva, urine, feces, semen, tears, or other body fluids or extracts thereof.

Inhibition of Na_vl .8 activity in a biological sample is useful for a variety of purposes that are known to one of skill in the art. Examples of such purposes include, but are not limited to, the study of sodium channels in biological and pathological phenomena; and the comparative évaluation of new sodium channel inhibitors.

SCHEMES AND EXAMPLES

The compounds of the invention may be prepared readily using the following methods. Ulustrated below in Scheme 1 is a general method for preparing the compounds of the présent invention.

Scheme 1: Préparation of Compounds of Formula I, where X is P(O)(OH)₂:

Page 57 oflOO

a) Alkoxy pyridine-4-amine (i.e. 2-methoxypyridin-4-amine), coupling agent (i.e. HATU, EDCI, HOBT), base (i.e. N-methylmorpholine, Et₃N), solvent (i.e., DMF, dichloromethane); (b) SO2CI2, DMF in a solvent (i.e., dichloromethane); (c) 2-Methoxypyridin-4-amine, base (i.e., pyridine), solvent (i.e., dichloromethane,

DMF); (d) TMSI or HBr, solvent (i.e., acetonitrile or acetic acid); (e) base (i.e., Cs₂CO₃, Na₂CO₃, K2CO₃, NaHCO₃), solvent (i.e., DMF, NMP, dioxane), heat; (f) CICO2CH2CI, solvent (i.e., CH₂C1₂, DMF); (g) Phosphorylating reagent (i.e. K(PGi)₂PO₄ including K(/-Bu)₂PO₄ and the like), (tî-Bu)₄NI, solvent (i.e., DMF), base (e.g., DIPEA), heat (i.e, 70°C); (h) HOAc, H₂O, solvent (i.e, CH₃CN), heat (i.e, 70°C).

One of skill in the art would recognize that steps (f) and (g) in Scheme 1 above may be combined into a single step without isolation of the intermediate chloride.

Page 58 oflOO

Scheme IA: Préparation of Compounds of Formula I, where X is P^iO^zîM*, X is P(O)(O')₂D²⁺ or

Xis P(O)(()H)OM⁺:

2-Me-THF, RT (i) (k)

M+OH- or D²⁺(OH-)₂

-----------►

X = PO(O-)₂ 2M⁺ or

X = PO(O-)₂ D²⁺ — X = PO(OH)₂

A_X=po(OH)O-M⁺ (i) M’OIT or D²⁺(OH')₂, 2-MeTHF; G) aq H; (k) aq M*OH’

Salts of compounds of formula I may be prepared as shown in Scheme 1 A. In step (i), a solution of compound I treated with ΜΓΌΗ' or D²⁺(OIT)2 provides the dianionic form of the compound (X = -PO(O)2·2Μ⁺ or -PO(O')2D²⁺). The free acid form of the compound (X = PO(OH)₂) may be obtained by treating the dianionic form with aqueous acid. The monoanionic form of the compound X = PO/OEQO'M^) may be prepared by treating the free acid form with one équivalent of M^OH'.

EXAMPLES

General methods. 'H NMR (400 MHz) and ³¹P NMR (162 MHz) spectra were obtained as solutions in an appropriate deuterated solvent such as dimethyl sulfoxide-d₆ (DMSO-d₆). Mass spectra (MS) were obtained using an Applied Biosystems API EX LC/MS System. Compound purity and rétention times were determined by reverse phase HPLC using a Kinetix Cl8 column (50 x 2.1 mm, 1.7 pm particle) from Phenomenex (pn: 00B-4475-AN)), and a dual gradient run from 1-99% mobile phase B over 3 minutes. Mobile phase A = H₂O (0.05 % CF₃CO₂H). Mobile phase B = CH₃CN (0.05 % CF₃CO₂H). Flow rate = 2 mL/min, injection volume = 3 pL, and column température = 50 °C. Silica gel chromatography was performed using silica gel-60 with a particle size of 230-400 mesh. Pyridine, dichloromethane (CH₂C1₂), tetrahydrofuran (THF), dimethylformamide (DMF), acetonitrile (ACN), methanol (MeOH), and 1,4-dioxane were from Baker or Aldrich and in some cases the reagents were Aldrich Sure-Seal bottles kept under dry

Page 59 of 100 nitrogen. Ail reactions were stirred magnetically unless otherwise noted. HATU stands for (1[bis(dimethylamino)methylene]-lH-l,2,3-triazolo[4,5-b]pyridinium-3-oxidehexafluorophosphate).

EXAMPLE 1

Préparation of 4-chloro-2-fluoro-N-(2-oxo-l ,2-dihydropyridin-4-yl)benzamide

A solution of 4-chloro-2-fluoro-benzoic acid (7.0 g, 40.10 mmol), HATU (15.25 g, 40.10 mmol), 2methoxypyridin-4-amine (4.98 g, 40.10 mmol) and Et₃N (22.4 mL, 160.4 mmol) in dichloromethane (63.0 mL) was stirred at room température ovemight. The crude mixture was purified by column chromatography eluting with a gradient of ethyl acetate in hexanes (0-50%) to yield 4-chloro-2-fluoro-N-(2-methoxy-4pyridyl)benzamide (4.35 g, 39%), as a white solid. ESI-MS m/z cale. 280.04, found 281.3 (M+l)⁺; Rétention time: 1.31 minutes (3 minutes run). Ή NMR (400 MHz, DMSO-d6) δ 10.80 (s, 1H), 8.09 (m, 1H), 7.73 (t, J = 8.0 Hz, 1H), 7.66 (dd, J= 10.1, 1.9 Hz, 1H), 7.46 (dd, J= 8.3, 1.9 Hz, 1H), 7.21 (m, 2H), 3.84 (s, 3H) ppm. To 4-chloro-2-fluoro-N-(2-methoxy-4-pyridyl)benzamide (4.35 g, 15.50 mmol) in acetonitrile (145.0 mL) was added TMSI (8.8 mL, 62.0 mmol). The reaction was stirred at 50°C ovemight. The acetonitrile was evaporated and the crude solid was triturated with ethyl acetate. The solid was isolated by filtration and washed with ethyl acetate to give 4-chloro-2-fluoro-N-(2-oxo-lH-pyridin-4-yl)benzamide (3.8 g, 92%). ESIMS m/z cale. 266.02, found 267.1 (M+l)+; Rétention time: 1.23 minutes (3 minutes run). *H NMR (400 MHz, DMSO-d₆) δ 10.86 (s, 1H), 7.73 (t, J = 8.0 Hz, 1H), 7.68 (dd, J = 10.1, 1.9 Hz, 1H), 7.60 (d, J = 7.1 Hz, 1H), 7.47 (dd, J= 8.3,2.0 Hz, 1H), 7.11 (d, J = 2.0 Hz, 1H), 6.71 (dd, J = 7.1, 2.1 Hz, 1H) ppm.

EXAMPLE 2

Préparation of 2-fluoro-N-(2-oxo-l ,2-dihydropyridin-4-yl)-4-(perfluoroethyl)benzamide

A solution of 4-bromo-2-fluoro-benzoyl chloride (2 g, 8.42 mmol) in dichloromethane (10.0 mL) was added drop-wise to a mixture of 2-methoxypyridin-4-amine (1.0 g, 8.42 mmol), pyridine (2.0 mL, 25.27 mmol) and dichloromethane (40.0 mL) at 0 °C. The mixture was allowed to warm to room température and was stirred at that température ovemight. The mixture was poured into IN HCl (200 mL) and dichloromethane (200 mL). The layers were separated and the organic layer was dried over sodium sulfate, filtered and

Page 60 of 100 concentrated under reduced pressure to give 4-bromo-2-fluoro-N-(2-methoxy-4-pyridyl)benzamide (1.2 g, 44%) as an off-white solid. ESI-MS m/z cale. 323.99, found 325.1 (M+l)⁺; Rétention time: 1.37 minutes (3 minutes run). 'H NMR (400 MHz, DMSO-d_â) δ 10.80 (s, 1H), 8.11 - 8.06 (m, 1H), 7.79 (dd, J = 9.8,1.7 Hz, 1H), 7.68 - 7.62 (m, 1H), 7.59 (dd, J = 8.3,1.7 Hz, 1H), 7.23 - 7.18 (m, 2H), 3.84 (s, 3H) ppm.

To a stirred solution of 4-bromo-2-fluoro-N-(2-methoxy-4-pyridyl)benzamide (800 mg, 2.46 mmol) and copper (1.6 g, 24.61 mmol) in DMSO (15 mL), in a pressure vessel was bubbled in l,l,l,2,2-pentafluoro-2iodo-ethane (4.1 g, 16.47 mmol) The vessel was sealed and heated at 120 °C for 16 hours. The reaction mixture was diluted with water and filtered through a plug of silica and then extracted with ethyl acetate (4x). The organics combined, washed with brine, dried over Na₂SO₄, filtered and evaporated to dryness to yield a crude mixture that was purified by column chromatography using a gradient of ethyl acetate in hexanes ( 0 -40%) to give 2-fluoro-N-(2-methoxy-4-pyridyl)-4-(l,l,2,2,2-pentafluoroethyl)benzamide (200 mg, 22%) as an off white solid. ESI-MS m/z cale. 364.06, found 365.3 (M+l)+; Rétention time: 1.39 minutes (3 minutes run). 'H NMR (400 MHz, DMSO-d₆) δ 10.98 (s, 1H), 8.11 (d, J = 6.3 Hz, 1H), 7.95 (dd, J = 7.4 Hz, 1H), 7.89 (d, J = 9.9 Hz, 1H), 7.72 (d, J = 9.1 Hz, 1H), 7.23 - 7.19 (m, 2H), 3.85 (s, 3H) ppm. 2-fhioro-N-(2-methoxy-4-pyridyl)-4-(l,l,2,2,2-pentafluoroethyl)benzamide (200 mg, 0.55 mmol) inHBr in acetic acid (1.3 mL of 33 %w/v, 5.49 mmol) was stirred at 100 °C for 2 hours, at this time 1 ml of HBr in acetic acid (33 %w/v ) was added and the mixture was stirred at 100 °C for 4 hours, then cooled to room température. The reaction mixture was diluted with water and a precipitate formed. The precipitate was filtered off, washed with water (2x), cold methyl-tert-butyl ether and dried under vacuum to give 2-fluoro-N(2-oxo-lH-pyridin-4-yl)-4-(l,l,2,2,2-pentafluoroethyl)benzamide (138 mg, 72%) as a light grey solid. ESIMS m/z cale. 350.05, found 351.3 (M+l)+; Rétention time: 1.3 minutes (3 minutes run).

EXAMPLE 3

Préparation of 4,5-dichloro-2-fluoro-N-(2-oxo-l ,2-dihydropyridin-4-yl)benzamide

A solution of 2-methoxypyridin-4-amine (186.2 mg, 1.5 mmol), 4,5-dichloro-2-fluoro-benzoic acid (285.1 mg, 1.36 mmol), HATU (622.4 mg, 1.64 mmol) and N-methylmorpholine (299.9 pL, 2.73 mmol) in DMF (3 mL) was stirred at room température for 16 hours. The reaction mixture was poured into water and extracted with ethyl acetate (3x). The organics were combined, washed with water (3x), brine and dried over Na₂SO₄, filtered through a short plug of silica and evaporated to dryness. The material was taken up in HBr (in acetic acid) (6.689 mL of 33 %w/v, 27.28 mmol) and stirred at 95 °C for 16 h. The solution was cooled to room température, filtered and solid product washed with water (2x) and then ether (2x) and dried under vacuum

Page 61 of 100 to give 4,5-dichloro-2-fluoro-N-(2-oxo-lH-pyridin-4-yl)benzamide (250 mg, 61%) as an off white solid.

ESI-MS m/z cale. 299.99, found 301.3 (M+l)⁺; Rétention time: 1.16 minutes (3 minutes run).

EXAMPLE 4

Préparation of 2-fluoro-N-(2-oxo-l,2-dihydropyridin-4-yl)-4-(trifluoromethyl)benzamide (19)

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A solution of 2-fluoro-4-(trifluoromethyl)benzoyl chloride (25.0 g, 110.3 mmol) in dichloromethane (125.0 mL) was added drop-wise to a mixture of 2-methoxypyridin-4-amine (13.7 g, 110.3 mmol), pyridine (26.8 mL, 330.9 mmol) and dichloromethane (500.0 mL) at 0 °C. The mixture was allowed to warm to room température and was stirred at that température ovemight. The mixture was poured into IN HCl (200 mL) and dichloromethane (200 mL). The layers were separated and the organic layer was dried over sodium sulfate, filtered and concentrated under reduced pressure. The product was slurried in hexane, the hexane was decanted and the product was dried under reduced pressure to yield 2-fluoro-N-(2-methoxy-4-pyridyl)-4(trifluoromethyl)benzamide (25.7 g, 74%) as a cream solid. ESI-MS m/z cale. 314.07, found 315.3 (M+l)⁺; Rétention time: 1.49 minutes (3 minutes run). *H NMR (400 MHz, DMSO-d₆) δ 10.96 (s, 1H), 8.15 - 8.04 (m, 1H), 8.00 - 7.85 (m, 2H), 7.76 (d, J = 8.1 Hz, 1H), 7.26 - 7.15 (m, 2H), 3.85 (s, 3H) ppm.

To 2-fluoro-N-(2-methoxy-4-pyridyl)-4-(trifluoromethyl)benzamide (1.00 g, 3.18 mmol) in acetic acid (6.0 mL) was added HBr (33% in acetic acid) (3.9 mL of 33 %w/v, 15.91 mmol) and the mixture stirred at 100 °C for 6 hours. Additional HBr (2 mL, 33% in acetic acid) was added and the mixture was stirred at room température ovemight. The mixture was then heated at 100 °C for 2 hours before it was cooled to room température. The mixture was partitioned between ethyl acetate and water. The layers were separated and the aqueous layer was extracted with ethyl acetate (3x). The combined organics were washed with water and brine (2x), dried over sodium sulfate, filtered and concentrated under reduced pressure. The solid was slurried in methyl-tert-butyl ether and filtered to give 2-fluoro-N-(2-oxo-lH-pyridin-4-yl)-4(trifluoromethyl)benzamide (19) (731 mg, 76 %). ESI-MS m/z cale. 301.05, found 301.3 (M+l)⁺; Rétention time: 1.35 minutes (3 minute run). 'H NMR (400 MHz, DMSO-d₆) δ 11.33 (s, 1H), 10.70 (s, 1H), 7.96 - 7.85 (m, 2H), 7.75 (d, J = 8.2 Hz, 1H), 7.35 (d, J = 7.2 Hz, 1H), 6.81 (d, J = 1.9 Hz, 1H), 6.41 (dd, J = 7.2, 2.1 Hz, 1H) ppm.

EXAMPLE 5

Préparation ofN-(2-oxo-l,2-dihydropyridin-4-yl)-2-(4-(trifluoromethoxy)phenoxy)-4(trifluoromethyl)benzamide (5a)

Page 62 of 100

Cs₂CO₃ (651.6 mg, 2 mmol) was added to a solution of 2-fluoro-N-(2-oxo-l,2-dihydropyridin-4-yl)-4(trifluoromethyl)benzamide (60.0 mg, 0.2 mmol) and 4-(trifluoromethoxy)phenol (259.1 pL, 2 mmol) in DMF (1 mL) and the reaction was stirred at 100°C for 1 hour. The reaction was filtered and purified by reverse phase HPLC using a gradient of acetonitrile in water (10-99%) and HCl as a modifier to yield N-(2oxo-1,2-dihydropyridin-4-yl)-2-(4-(trifluoromethoxy)phenoxy)-4-(trifluoromethyl)benzamide (5a) (25.7 mg, 28%). ESI-MS m/z cale. 458.07, found 459.5 (M+l)⁺; Rétention time: 1.80 minutes (3 minutes run)

EXAMPLE 6

Préparation of 2-(4-fluoro-2-methylphenoxy)-N-(2-oxo-l ,2-dihydropyridin-4-yl)-4(trifluoromethyl)benzamide (9a)

A mixture of 2-fluoro-N-(2-oxo-lH-pyridin-4-yl)-4-(trifluoromethyl)-benzamide (13.6 g, 45.30 mmol), 4fluoro-2-methyl-phenol (17.1 g, 135.9 mmol), Cs₂CO₃ (44.28 g, 135.9 mmol) and DMF (340.0 mL) was heated at 100 ° C for 1.5 hours. The mixture was cooled to room température and was poured into water (500 mL). The mixture was stirred vigorously for 30 min before it was filtered. The solid was washed with water (250 mL) and was slurried with methyl tert-buthyl ether (200 mL). The mixture was filtered and the solid was slurried with hexanes (2 x 400 mL) and the filtrate was dried under vacuum to give 2-(4-fluoro-2methyl-phenoxy)-N-(2-oxo-lH-pyridin-4-yl)-4-(trifluoromethyl)benzamide (9a) (13.1 g, 70 %) as a solid. ESI-MS m/z cale. 406.09, found 407.5 (M+l)⁺; Rétention time: 1.73 minutes (3 minutes run). 'H NMR (400 MHz, DMSO-d₆) δ 11.28 (s, 1H), 10.63 (s, 1H), 7.84 (d, J = 7.8 Hz, 1H), 7.60 (d, J = 7.1 Hz, 1H), 7.31 (d, J = 7.2 Hz, 1H), 7.26 - 7.20 (m, 1H), 7.14 - 7.06 (m, 2H), 7.00 - 6.95 (m, 1H), 6.75 (d, J = 1.8 Hz, 1H), 6.38 (dd, J = 7.2,2.1 Hz, 1H), 2.16 (s, 3H) ppm.

Page 63 of 100

EXAMPLE 7

Préparation of 2-(4-fluoro-2-methoxyphenoxy)-N-(2-oxo-l ,2-dihydropyridin-4-yl)-5(trifluoromethyl)benzamide (4a)

Cs₂CO₃ (651.6 mg, 2.0 mmol) was added to a solution of 2-fluoro-N-(2-oxo-l,2-dihydropyridin-4-yl)-5(trifluoromethyl)benzamide (60.0 mg, 0.2 mmol) and 4-fluoro-3-methoxyphenol (228 μΐ, 2.0 mmol) in DMF (1 mL) and the reaction was stirred at 100°C for 1 hour. The reaction was filtered and purified by reverse phase HPLC using a gradient of acetonitrile in water (10-99%) and HCl as a modifier to yield 2-(4-fluoro-2methoxyphenoxy)-N-(2-oxo-l,2-dihydropyridin-4-yl)-5-(trifluoromethyl)benzamide (4a) (67.9 mg, 80%).

ESI-MS m/z cale. 422.09, found 423.2 (M+l)⁺; Rétention time: 1.56 minutes (3 minutes run).

Following a similar procedure as described above for parent compound 4a, the following compounds were prepared from 2-fluoro-N-(2-oxo-l,2-dihydropyridin-4-yl)-5-(trifluoromethyl)benzamide and the following alcohols.

Cmpd No.	Product	Alcohol
18a	2-(4-fluorophenoxy)-N-(2-oxo-l,2dihydropyridin-4-yl)-5(trifluoromethyl)benzamide	4-fluorophenol
15a	N-(2-oxo-l,2-dihydropyridin-4-yl)-2-(o-tolyloxy)- 5-(trifluoromethyl)benzamide	2-methylphenol
lia	2-(2-chloro-4-fluorophenoxy)-N-(2-oxo-l,2dihydropyridin-4-yl)-5- (trifluoromethyl)benzamide	2-chloro-4-fluoro-phenol
10a	2-(4-fluoro-2-methylphenoxy)-N-(2-oxo-l,2dihydropyridin-4-yl)-5- (trifluoromethyl)benzamide	4-fluoro-2-methyl-phenol
8a	N-(2-oxo-l,2-dihydropyridin-4-yl)-2-(4- (trifluoromethoxy)phenoxy)-5- (trifluoromethyl)benzamide	4-(trifluoromethoxy)phenol
17a	N-(2-oxo-l,2-dihydropyridin-4-yl)-2-(2- (trifluoromethoxy)phenoxy)-5- (trifluoromethyl)benzamide	2-(trifluoromethoxy)phenol

EXAMPLE 8

Page 64 of 100

Préparation of 5-chloro-2-(4-fluoro-2-methoxyphenoxy)-N-(2-oxo-l ,2-dihydropyridin-4-yl)benzamide (7a)

CS2CO3 (879.9 mg, 2.7 mmol) was added to a solution of 2-fluoro-N-(2-oxo-l,2-dihydropyridin-4-yl)-5chlorobenzamide (72.0 mg, 0.27 mmol) and 4-fluoro-3-methoxyphenol (307.7 μΐ, 2.7 mmol) in DMF (1 mL) and the reaction was stirred at 100°C for 1 hour. The reaction was filtered and purified by reverse phase HPLC using a gradient of acetonitrile in water (10-99%) and HCl as a modifier to yield 5-chloro-2-(4-fluoro2-methoxyphenoxy)-N-(2-oxo-l,2-dihydropyridin-4-yl)benzamide (7a) (31.8 mg, 30%). ESI-MS m/z cale. 388.06, found 389.10 (M+l)⁺; Rétention time: 1.52 minutes (3 minutes run).

Following a similar procedure as described above for parent compound 7a, the following compounds were prepared from 5-chloro-2-(4-fluoro-2-methoxyphenoxy)-N-(2-oxo-l ,2-dihydropyridin-4-yl)benzamide and the following alcohols.

Cmpd No.	Product	Alcohol
12a	5-chloro-2-(4-fluoro-2-methylphenoxy)-N-(2- oxo-1,2-dihydropyridin-4-yl)benzamide	4-fluoro-2-methyl-phenol
14a	5-chloro-2-(2-chloro-4-fluorophenoxy)-N-(2- oxo-1,2-dihydropyridin-4-yl)benzamide	2-chloro-4-fluoro-phenol

EXAMPLE 9

Préparation of 4,5-dichloro-2-(4-fluoro-2-methoxyphenoxy)-N-(2-oxo-l,2-dihydropyridin-4-yl)benzamide (la)

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^F

Cs₂CO₃ (97.7 mg, 0.3 mmol) was added to a solution of 4,5-dichloro-2-fluoro-N-(2-oxo-l,2-dihydropyridin4-yl)benzamide (30.1 mg, 0.1 mmol) and 4-fluoro-3-methoxyphenol (42.6 mg, 0.3 mmol) in NMP (0.5 mL)

Page 65 of 100 and the reaction was stirred at 90°C for 2 hours. The reaction was filtered and purified by reverse phase HPLC using a gradient of acetonitrile in water (1-99%) and HCl as a modifier to yield 4,5-dichloro-2-(4fluoro-2-methoxyphenoxy)-N-(2-oxo-l,2-dihydropyridin-4-yl)benzamide (la) (13.2 mg, 30%). ESI-MS m/z cale. 422.02, found 423.3 (M+l)⁺; Rétention time: 1.57 minutes (3 minutes run).

Following a similar procedure as described above for parent compound la, the following compounds were prepared from 4,5-dichloro-2-fluoro-N-(2-oxo-l,2-dihydropyridin-4-yl)benzamide and the following alcohols.

Cmpd No.	Product	Alcohol
3a	4,5-dichloro-2-(4-fluorophenoxy)-N-(2-oxo- 1,2-dihydropyridin-4-yl)benzamide	4-fluorophenol

EXAMPLE 10

Préparation of 2-(4-fluoro-2-methoxyphenoxy)-N-(2-oxo-l ,2-dihydropyridin-4-yl)-4(perfluoroethyl)benzamide (2a)

Cs₂CO₃ (69.8 mg, 0.21 mmol) was added to a solution of 2-fluoro-N-(2-oxo-l,2-dihydropyridin-4-yl)-4(perfluoroethyl)benzamide (25 mg, 0.07 mmol) and 4-fluoro-2-methoxyphenol (24.4 pL, 0.2 mmol) in NMP (0.3 mL) and the reaction was stirred at 100°C for 45 minutes. The reaction mixture was poured into water : ethyl acetate (9:1). The mixture was shaken, and the solid was filtered off, washed with ether, then triturated with ethyl acetate and dried to give the desired product. The mother liquors were filtered and washed with ethyl acetate to give a second crop of material. Both solids were combined and dried under vacuum to give 2-(4-fluoro-2-methoxy-phenoxy)-N-(2-oxo-lA-pyridin-4-yl)-4-(l,l,2,2,2-pentafluoroethyl)benzamide (2a) (15.4 mg, 45%) as a white solid. ESI-MS m/z cale. 472.08, found 473.3 (M+l)+; Rétention time: 1.62 minutes. Ή NMR (400 MHz, DMSO-d₆) δ 11.32 (s, 1H), 10.62 (s, 1H), 7.83 (d, J = 7.9 Hz, 1H), 7.51 (d, J = 7.9 Hz, 1H), 7.39 - 7.27 (m, 2H), 7.17 (dd, J= 10.7,2.8 Hz, 1H), 6.88 (dd, J = 11.3, 5.7 Hz, 1H), 6.81 (s, 1H), 6.75 (s, 1H), 6.43 (d, J = 7.1 Hz, 1H), 3.73 (s, 3H) ppm.

Page 66 of 100

EXAMPLE 11

Préparation of 4-chloro-2-(4-fluoro-2-methylphenoxy)-N-(2-oxo-l ,2-dihydropyridin-4-yl)benzamide (13 a)

To a solution of 4-chloro-2-fluoro-N-(2-oxo-lH-pyridin-4-yl)benzamide (500 mg, 1.87 mmol) in NMP (5 mL) was added 4-fluoro-2-methyl-phenol (709.5 mg, 5.62 mmol) and Cs₂CO₃ (1.83 g, 5.62 mmol) and the reaction mixture was stirred at 90 °C for 2 hours. The reaction mixture was poured into water and extracted with ethyl acetate (3x). The organics were combined, washed with 3N NaOH (3x), water, brine, dried (Na₂SO₄) and evaporated to dryness. Purification by silica gel column chromatography gave 4-chloro-2-(4fluoro-2-methyl-phenoxy)-N-(2-oxo-177-pyridin-4-yl)benzamide (13a) (300 mg, 43%) as a tan solid. ESI-MS m/z cale. 372.07, found 373.1 (M+l)⁺; Rétention time: 1.5 minutes (3 minutes run). 'El NMR (400 MHz, DMSO-d₆) δ 11.25 (s, 1H), 10.48 (s, 1H), 7.65 (d, J = 8.2 Hz, 1H), 7.30 (dd, J = 7.9,1.6 Hz, 2H), 7.21 (d, J = 8.7 Hz, 1H), 7.09 (d, J = 5.5 Hz, 2H), 6.75 (dd, J = 5.1,1.8 Hz, 2H), 6.38 (dd, J = 7.2, 2.0 Hz, 1H), 2.16 (s, 3H) ppm.

EXAMPLE 12

Préparation of 2-(2,4-difluorophenoxy)-N-(2-oxo-l,2-dihydropyridin-4-yl)-4-(trifluoromethyl)benzamide

To a solution of2-fluoro-N-(2-oxo-l,2-dihydropyridin-4-yl)-4-(trifluoromethyl)benzamide (30 mg, 0.1 mmol) in DMF (1 mL) was added 2,4-difluorophenol (130 mg, 1.0 mmol) and Cs₂CO₃ (325.8 m g, 1.0 mmol) and

Page 67 of 100 the reaction mixture was stirred at 100 °C for 1 hour. The reaction was cooled to 25 °C, filtered and purified by reverse phase chromatography using a gradient of acetonitrile in water (10-99%) and HCl as a modifier to yield 2-(2,4-difluorophenoxy)-N-(2-oxo-l,2-dihydropyridin-4-yl)-4-(trifluoromethyl)benzamide (16). ESIMS m/z cale. 410.07, found 411.2 (M+l)+; Rétention time: 1.55 minutes (3 minutes run).

EXAMPLE 13

Préparation of N-[l -(chloromethyl)-2-oxo-4-pyridyl]-2-(4-fluoro-2-methyl-phenoxy)-4(trifhioromethyl)benzamide (20)

A solution of 2-(4-fluoro-2-methylphenoxy)-N-(2-oxo-l,2-dihydropyridin-4-yl)-4(trifluoromethyl)benzamide (9a) (406.3 mg, 1.0 mmol) and chloromethyl chloroformate (106.7 pL, 1.2 mmol) in dichloromethane (3.5 mL) and N,N-dimethylformamide (0.3 mL) was stirred at room température for 4 hours. The reaction mixture was diluted with ethyl acetate, washed with saturated aqueous sodium bicarbonate, brine, dried with Na₂SO₄, filtered and evaporated to dryness to give N-[l-(chloromethyl)-2-oxo4-pyridyl]-2-(4-fluoro-2-methyl-phenoxy)-4-(trifluoromethyl)benzamide that was used in the next step without further purification. ESI-MS m/z cale. 454.07, found 455 (M+l)⁺; Rétention time: 0.73 minutes (1 minute run).

The crude N-[l-(chloromethyl)-2-oxo-4-pyridyl]-2-(4-fluoro-2-methyl-phenoxy)-4(trifluoromethyl)benzamide from the previous step was taken up in N,N-dimethylformamide (5 mL). Oï-tertbutoxyphosphoryloxypotassium (496.6 mg, 2.0 mmol) and tetrabutylammonium iodide (36.94 mg, 0.10 mmol) were added and the reaction mixture was stirred at 70 °C for 4 hours. The reaction mixture was then diluted with ethyl acetate and washed with water then brine, dried with Na₂SO₄, filtered and evaporated to dryness. The material was purified by column chromatography (40g silica, 50 - 100% EtOAc in Hexanes), fractions with the product were pooled and evaporated to give di-teri-butyl [4-[[2-(4-fluoro-2-methylphenoxy)-4-(trifluoromethyl)benzoyl]amino]-2-oxo-l-pyridyl]methyl phosphate (20) (280 mg, 45%) as a yellow oil that was used in the next step without further purification. ESI-MS m/z cale. 628.20, found 629 (M+l)⁺; Rétention time: 0.76 minutes (1 minute run).

Page 68 of 100

EXAMPLE 14

Préparation of [4-[[2-(4-fhioro-2-methyl-phenoxy)-4-(trifluoromethyl)benzoyl]amino]-2-oxo-l pyridyljmethyl dihydrogen phosphate (9)

A solution of di-teri-butyl [4-([2-(4-fluoro-2-methyl-phenoxy)-4-(trifluoromethyl)benzoyl]amino]-2-oxo-lpyridyljmethyl phosphate (20) (50 mg, 0.080 mmol) in acetonitrile (1 mL), H2O (1 mL), and acetic acid (1 mL) was stirred at 70 °C for 3 hours then evaporated to dryness. The material was then co-evaporated with acetonitrile (3x), triturated with acetonitrile, filtered, washed with acetonitrile and desiccated to give [4-((2(4-fhioro-2-methyl-phenoxy)-4-(trifluoromethyl)benzoyl]amino]-2-oxo-l-pyridyl]methyl dihydrogen phosphate (9) (20 mg, 49%) as a white solid. ‘H NMR (400 MHz, DMSO-d₆) δ 11.49 (s, 2H), 10.77 (s, 1H), 7.86 (d, J =8.0 Hz, 1H), 7.61 (d, J = 7.6 Hz, 2H), 7.27-7.19 (m, 1H), 7.11 (dd, J=10.9,7.6Hz,2H), 6.98 (s, 1H), 6.90 (d, J = 2.0 Hz, 1H), 6.43 (dd, J = 7.5,2.1 Hz, 1H), 5.53 (d, J = 9.7 Hz, 2H), 2.16 (s, 3H) ppm. ^3IP NMR (162 MHz, DMSO-d₆ - 85% H3PO4 aq. as internai standard - 0 ppm) δ -1.76 (t, J = 9.6 Hz, 1H).

The material was further purified according to the following procedure. To a stirred suspension of [4-((2-(4fluoro-2-methyl-phenoxy)-4-(trifluoromethyl)benzoyl]amino]-2-oxo-l-pyridyl]methyl dihydrogen phosphate (9) (30.00 mg, 0.05810 mmol) in isopropanol (600.0 pL) was added methanol (300.0 pL) which caused the material to go into solution. NaOH (14.52 pL of 4 M, 0.058 mmol) was added which caused a white precipitate and the mixture was stirred at room température for 30 minutes. The reaction mixture was then cooled to 0 °C, filtered, washed with cooled isopropanol and dried under vacuum to give (4-[(2-(4-fluoro-2methyl-phenoxy)-4-(trifluoromethyl)benzoyl]amino]-2-oxo-l-pyridyl]methyl dihydrogen phosphate (9) as a white solid. ESI-MS m/z cale. 516.07, found 517.0 (M+l)+; Rétention time: 0.57 minutes (1 min UPLC run).

EXAMPLE 15

Altemate préparation of (4-([2-(4-fluoro-2-methyl-phenoxy)-4-(trifluoromethyl)benzoyl]amino]-2-oxo-lpyridyljmethyl dihydrogen phosphate (9)

A sample of 2-(4-fluoro-2-methylphenoxy)-N-(2-oxo-l ,2-dihydropyridin-4-yl)-4-(trifluoromethyl)benzamide monohydrate (9a) (6.0 g, 14.7 mmol) was dehydrated under vacuum at 65 °C. A solution of anhydrous 2-(4Page 69 of 100 fluoro-2-methylphenoxy)-N-(2-oxo-l,2-dihydropyridin-4-yl)-4-(trifluoromethyl)benzamide (9a) (5.0 g, 12.31 mmol) and N,N-dimethylformamide (10.0 mL) in dichloromethane (50.0 mL) was stirred at room temp., chloromethyl chloroformate (1.64 ml, 18.46 mmol) was added and the solution was stirred at room température for one hour. The reaction mixture was diluted with ethyl acetate, washed with saturated aqueous sodium bicarbonate, brine, dried with anhydrous sodium sulfate, filtered and evaporated to dryness. The resulting oil was purified by column chromatography (40g silica, 20 -100% EtOAc in Hexanes), product fractions were pooled and evaporated to give N-[l-(chloromethyl)-2-oxo-4-pyridyl]-2-(4-fluoro-2methyl-phenoxy)-4-(trifluoromethyl)benzamide (3.26g, 7.168 mmol) that was used in the next step without further purification. *H NMR (400 MHz, DMSO-d₆) δ 10.83 (s, IH), 7.86 (d, J = 7.8 Hz, IH), 7.76 (d, J = 7.6 Hz, IH), 7.66 - 7.58 (m, IH), 7.28 - 7.19 (m, IH), 7.10 (dd, J = 7.7, 2.0 Hz, 2H), 6.98 (s, IH), 6.92 (d, J = 2.1 Hz, IH), 6.50 (dd, J = 7.6, 2.3 Hz, IH), 5.79 (s, 2H), 2.16 (s, 3H) ppm.

A mixture of N-[l -(chloromethyl)-2-oxo-4-pyridyl]-2-(4-fluoro-2-methyl-phenoxy)-4(trifluoromethyl)benzamide (500 mg, 1.1 mmol), di-tert-butoxyphosphoryloxy potassium (409.2 mg, 1.65 mmol), tetrabutylammonium iodide (20.3 mg, 0.05 mmol) and diisopropylethyl amine (191.4 ul, 1.1 mmol) in acetonitrile (10.0 ml) were stirred at 70 °C for one hour. The reaction mixture was then diluted with ethyl acetate and washed with water and brine and the organic layer was evaporated to dryness. The crude material was purified by column chromatography (4g silica, 25 -100% EtOAc in Hexanes), product fractions were pooled and evaporated to give di-teri-butyl [4-[[2-(4-fluoro-2-methyl-phenoxy)-4(trifluoromethyl)benzoyl]amino]-2-oxo-l-pyridyl]methyl phosphate (20) (390 mg, 56%) as a light amber solid. ‘H NMR (400 MHz, DMSO-d₆) δ 10.83 (s, IH), 7.86 (d, J = 7.8 Hz, IH), 7.76 (d, J = 7.6 Hz, IH), 7.66 - 7.58 (m, IH), 7.28 - 7.19 (m, IH), 7.10 (dd, J = 7.7,2.0 Hz, 2H), 6.98 (s, IH), 6.92 (d, J = 2.1 Hz, IH), 6.50 (dd, J = 7.6, 2.3 Hz, IH), 5.79 (s, 2H), 2.16 (s, 3H) ppm.

A solution of di-teri-butyl [4-[[2-(4-fluoro-2-methyl-phenoxy)-4-(trifluoromethyl)benzoyl]amino]-2-oxo-lpyridyljmethyl phosphate (20) (350 mg, 0.557 mmol) in acetonitrile (7 mL), acetic acid (7 mL), and H₂O (7 mL) was stirred at 70 °C and the deprotection reaction followed by HPLC. Deprotection was complété after 1.5 hours. The reaction mixture was partially concentrated to remove the bulk of the acetonitrile then toluene (100 ml) was added and the mixture evaporated to azeotropically remove water and acetic acid. To the resulting suspension was added heptane (10 ml) and the suspension distilled down to 2-3 volumes (1ml). Heptane (2ml) was added and the suspension further stirred at room temp and filtered. The collected solid was dried in vacuo to afford [4-[[2-(4-fluoro-2-methyl-phenoxy)-4-(trifluoromethyl)benzoyl]amino]-2-oxo-lpyridyl]methyl dihydrogen phosphate (9) (254 mg, 88.4 %) as off-white crystalline solid. *H NMR (400 MHz, DMSO-d₆) δ 11.52 (s, 2H), 10.77 (s, IH), 7.86 (d, J = 8.0 Hz, IH), 7.60 (d, J = 7.6 Hz, 2H), 7.26 - 7.19 (m, IH), 7.09 (dd, J =10.9, 7.6Hz,2H), 6.98 (s, IH), 6.90 (d, J = 2.0 Hz, IH), 6.43 (dd, J = 7.5,2.1 Hz, IH), 5.53 (d, J = 9.7 Hz, 2H), 2.16 (s, 3H) ppm. ³¹P NMR (162 MHz, DMSO-d₆) δ -2.35 (t, J = 9.6 Hz, IH) ppm.

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EXAMPLE 15A

Préparation of 2-fluoro-N-(2-methoxypyridin-4-yl)-4-(trifluoromethyl)benzamide

A 50 liter jacketed glass reactor was fitted with an N₂ inlet, a mechanical stirrer, and a condenser. With the stirrer set to 150 rpm and the jacket température set at 40 °C, 2-Me-THF (6.000 L, 3.0 vol), 2-fluoro-4(trifluoromethyl)benzoic acid (2000 g, 9.610 mol), 2-methoxypyridin-4-amine (1.278 kg, 10.09 mol), and TEA (2.917 kg, 4.018 L, 28.83 mol) were added to the reactor, which resulted in a slightly hazy, light amber solution. The reactor was switched to reaction control and heated to 35 °C. To the solution was added T3P in 2-Me-THF (9.176 kg, 9.176 L of 50 %w/w, 14.42 mol) over 30-45 min, which resulted in a light amber solution. After 2 hours, the reaction was judged to be complété by HPLC analysis (<2% of 1 remaining). The reaction was quenched with water (1.000 L, 0.5 vol), which was added via addition formel over a period of 10 minutes in order to control the exothermic quenching reaction. The mixture was then diluted with 2Me-THF (8.000 L, 4.0 vol) and water (8.000 L, 4.0 vol) and stirred for 30 minutes at 30-40 °C. After stirring was stopped, the layers were allowed to separate, and the aqueous layer was removed. The organic layer was washed with 10% aqueous NaOH (6.000 L, 3.0 vol), stirring resulted in an émulsion. Brine (500.0 mL, 0.25 vol) was added, and the mixture was stirred for about 5 minutes. The layers were separated, and the aqueous layer removed. The organic layer was washed again with brine (10.00 L, 5.0 vol), and the aqueous layer was drained. The organic layer was dried over Na₂SO₄, and filtered through diatomaceous earth (Celite). The filter cake was washed with 2-Me-THF (4.000 L, 2.0 vol) and pulled dry. The filtrate was transferred to a rotovap, and partial distillation of solvent was begun at a bath température of 40 °C and a pressure of 150 mbar, resulting in the formation of solids in the mixture. Cyclohexane (10.00 L, 5.0 vol) was added portionwise during the partial distillation. Distillation was stopped, the reaction mixture (~8 liter) was slurried on the rotovap, and the bath température was reduced to room température. The mixture was filtered, and the filter cake was washed with cyclohexane (2.000 L, 1.0 vol) and pulled dry under a nitrogen blanket to afford a light yellow solid. The solid was scooped out of the fonnel and dried in vacuo (40 °C, <30mbar, rotovap) to afford 2-fluoro-N-(2-methoxypyridin-4-yl)-4-(trifluoromethyl)benzamide (2,501 g, 7.959 mol, 83%) as a fine, off-white solid.

EXAMPLE 15B

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Préparation of 2-fluoro-N-(2-oxo-l,2-dihydropyridin-4-yl)-4-(trifluoromethyl)benzamide hydrobromide

To a 50 liter jacketed glass reactor fitted with an N₂ inlet and a mechanical stirrer set was added AcOH (17.50 L, 7.0 vol) and 2-fluoro-N-(2-methoxypyridin-4-yl)-4-(trifluoromethyl)benzamide (2500 g, 7.956 mol), and the resulting mixture was stirred. A solution of HBr in HOAc (5.853 kg, 3.928 L of 33 %w/w, 23.87 mol) was added, resulting in a mild exotherm and a light amber solution. The solution became a darker amber color as more HBr was added. The température of the reaction mixture was increased to a mild reflux (70 °C internai), over ~30 min, resulting in the génération of a substantial amount of gas (MeBr, HBr). The internai température of the reaction mixture was then increased to 85 °C over 1.5 hrs, and stirring was continued overnight at a température of 85 °C. The progress of the reaction was monitored by HPLC analysis until completion, which was achieved after about 16 hours (<1% of 2-fluoro-N-(2-methoxypyridin4-yl)-4-(trifluoromethyl)benzamide remaining relative to the product 2-fluoro-N-(2-oxo-l,2-dihydropyridin4-yl)-4-(trifluoromethyl)benzamide hydrobromide). The internai température of the reaction mixture was reduced from 85 to 50 °C over 30 min, and then toluene (7.500 L, 3.0 vol) was added. Stirring was continued for 10-15 min. The internai température of the reaction mixture was then reduced to 20 °C, and the mixture was stirred at this température for 1-2 hrs. The reaction mixture was then filtered, and the wet filter cake was washed with toluene (7.500 L, 3.0 vol) and pulled dry. The solid material was scooped out of the filter and dried in vacuo (40 °C, 10-25 mbar, rotovap) to afford 2-fluoro-N-(2-oxo-l,2-dihydropyridin-4yl)-4-(trifluoromethyl)benzamide hydrobromide (2609 g, 6.846 mol, 86 %) as white, crystalline solid.

EXAMPLE 15C

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9a

To a 50 liter jacketed glass reactor fïtted with an N₂ inlet and a mechanical stirrer was added l-methyl-2pyrrolidinone (NMP) (3.75 liters). The solution was stirred, 2-fluoro-N-(2-oxo-l,2-dihydropyridin-4-yl)-4(trifluoromethyl)benzamide hydrobromide sait (1500.2 g, 3.94 moles, 1.0 eq) was added and chased with NMP (1 liter), and the jacket température was adjusted to 35°C. Potassium carbonate (1631.9 g, 11.8 moles, 3.0 eq, 325 mesh) was then added portionwise over 10 minutes, during which time the reaction température increased to 40 °C. The resulting suspension was treated with a solution of 4-fluoro-2-methylphenol (5, 546.1 g, 4.33 moles, 1.1 eq, AK Scientific) in NMP (2.25 liters) with stirring, and the addition funnel was then rinsed with NMP (0.75 liters) to give an orange suspension. The jacket température was raised to 61 °C over 30 minutes and the suspension was stirred ovemight under nitrogen, at which time the reaction was judged to be complété by HPLC analysis. To the reaction mixture was added 2-methyltetrahydrofuran (15 liters) and water (15 liters) and the mixture stirred until ail solids dissolved. Stirring was stopped, the orange aqueous layer drained off, and the organic layer washed with water (7.5 liters) while stirring and a jacket température of ~ 52 °C). The aqueous wash procedure was repeated 4 times (3 X 7.5 liter water washes, then 1 X 4.5 liter water wash). The resulting organic slurry was stirred at a jacket température of 50.8 °C, and isopropyl acetate (6 liters, Sigma Aldrich) was added. The jacket température was ramped down to 20 °C over 30 minutes, and the slurry was stirred ovemight before collecting the precipitated solid by filtration. The collected solid was retumed to the reactor, slurried in isopropyl acetate with stirring for about 2 hours, then filtered, rinsed with isopropyl acetate (1.5 liters) and dried in vacuo at 65 °C to give 1253.1 g (78%) of 2-(4-fluoro-2-methylphenoxy)-N-(2-oxo-l ,2-dihydropyridin-4-yl)-4-(trifluoromethyl)benzamide (9a) as an off-white solid.

EXAMPLE 15D

Préparation ofN-(l-(chloromethyl)-2-oxo-l,2-dihydropyridin-4-yl)-2-(4-fluoro-2-methylphenoxy)-4(trifluoromethyl)benzamide

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cf₃ cf₃

9a

To a 50 liter jacketed glass reactor fitted with an N₂ inlet and a mechanical stirrer, and with a jacket température set at 20 °C, was added 2-(4-fluoro-2-methylphenoxy)-N-(2-oxo-l,2-dihydropyridin-4-yl)-4(trifluoromethyl)benzamide (9a, 2482.0g, 6.11 moles, 1.0 eq) under nitrogen. Ethyl acetate (25 liters) was added with stirring at 100 rpm followed by 1,4-diazabicyclo [2.2.2] octane (DABCO) (342.6 g, 3.05 moles, 0.5 eq) and DMF (1.25 liters, Sigma-Aldrich). Chloromethyl chloroformate (815 ml, 9.16 moles, 1.5 eq) was then added over 30 minutes. When the addition of chloromethyl chloroformate was complété, the jacket température was ramped up to 60 °C over 30 minutes. The resulting yellow-slurry was stirred for 3 hours at about 60 °C, at which time the reaction was judged to be complété by HPLC analysis. The jacket température was ramped down to 15 °C over 20 minutes before quenching the reaction by slow addition of water (500 ml) over 10 minutes. Additional water was added, and the mixture stirred at 115 rpm for 15 minutes. Stirring was stopped, the aqueous layer was discarded, and the organic layer washed with water (5 liters), followed by a saturated solution of NaHCO₃ (137 g) in water (620 ml). The organic layer was seeded with 5 g of N-(l-(chloromethyl)-2-oxo-l,2-dihydropyridin-4-yl)-2-(4-fluoro-2-methylphenoxy)-4(trifluoromethyl)benzamide (7), and the resulting slurry was partially concentrated (removed 18.5 liters of organics) in the rotovap (40 °C, vacuum). The resulting suspension was stored at room température under nitrogen atmosphère overnight, during which time additional material crystallized out of solution. The remaining solvent was chased with heptanes adding more heptanes as needed to maintain the volume at 10 liters. The thick suspension was stirred on the rotovap at room température for 45 minutes, and then the solids were collected by filtration. The off-white solid was washed with heptanes (2.5 liters), then dried in vacuo (40 °C, fall house vacuum) to give 2409 g (87%) of N-(l-(chloromethyl)-2-oxo-l,2-dihydropyridin-4yl)-2-(4-fluoro-2-methylphenoxy)-4-(trifluoromethyl)benzamide as an off-white crystalline solid.

EXAMPLE 15E

Préparation of di-ter/-butyl ((4-(2-(4-fluoro-2-methylphenoxy)-4-(trifluoromethyl)benzamido)-2-oxopyridinl(2H)-yl)methyl) phosphate (20)

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CK^OtBii ?OtBu

CF₃ 20

To a 50 liter jacketed glass reactor fitted with an N₂ inlet and a mechanical stirrer, and with a jacket température set at 41°C, was addedN-(l-(chloromethyl)-2-oxo-l,2-dihydropyridin-4-yl)-2-(4-fluoro-2methylphenoxy)-4-(trifluoromethyl)benzamide (1199.5 g, 2.64 moles, 1.0 eq) under nitrogen. Ethyl acetate (12 liters) was added with stirring to produce a suspension. To the mixture was added potassium àï-tertbutylphosphate (792.7 g @ 95% purity, 3.03 moles, 1.15 eq), then TBAI (9.7 g, 0.026 moles, 0.01 eq), and the jacket température was ramped to 71 °C over 20 minutes. The resulting gelatinous suspension was stirred for 4.5 hours at which point HPLC analysis indicated that the reaction was complété. The jacket température was ramped to 30°C over 15 minutes, and then water (6 liters) was added with stirring. The aqueous layer was drained off, and then the organic layer was washed twice with water (1 X 3.6 liters, then 1 X 2.4 liters). The organic layer was concentrated down to 3.0-3.5 volumes at 40°C using a rotovap. Heptane (1.8 liters) was added as an antisolvent, and then the bath heater of the rotovap was tumed off, and the mixture was allowed to cool to room temp and was stirred at 40 rpm overnight. The solids were collected by filtration, rinsed with heptanes (1.2 liters), and then dried in vacuo at 45 °C to give 1417.7g (88%) of di-te/7-butyl ((415 (2-(4-fluoro-2-methylphenoxy)-4-(trifluoromethyl)benzamido)-2-oxopyridin-l (2H)-yl)methyl) phosphate (20) as a crystalline, light amber solid.

EXAMPLE 15F

Préparation of (4-(2-(4-fluoro-2-methylphenoxy)-4-(trifluoromethyl)benzamido)-2-oxopyridin-1 (2H)yl)methyl dihydrogen phosphate (9)

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To a 72 liter jacketed glass reactor fitted with an N₂ inlet and a mechanical stirrer, and with a jacket température set at 40 °C, was added di-ieri-butyl ((4-(2-(4-fluoro-2-methylphenoxy)-4(trifluoromethyl)benzamido)-2-oxopyridin-l(2H)-yl)methyl) phosphate (20,2820.9 g, 4.49 moles, 1.0 eq) and isopropyl alcohol (25.4 liters, 9.0 volumes). The mixture was stirred at 200 rpm, and acetic acid (14.1 liters, 5.0 volumes) was added, resulting in a clear solution. The clear solution was polish filtered and transferred to a 50 liter jacketed glass reactor System with stirring at 100 rpm. Water (5.6 liters) was added, and thejacket température was ramped to 71 °C over 20 minutes. After 4.5 hours of stirring and heating, HPLC analysis indicated that the reaction was complété. The jacket température was ramped down to 19 °C over 3 hours, and the product began crystallizing out of solution. The solid was collected by filtration, rinsed with acetone (5 liters). The solid was added back into the reactor vessel, acetone was added (8.5 liters), the jacket température was ramped to 45 °C over 10 minutes, and the suspension was stirred. After 40 minutes, the jacket température was ramped to 20 °C over 30 minutes, and the crystalline solid was collected by filtration, rinsed with acetone (5 liters) and dried in vacuo at 50 °C to give 1917.7 g (83%) of (4-(2-(4-fluoro15 2-methylphenoxy)-4-(trifluoromethyl)benzamido)-2-oxopyridin-l(2H)-yl)methyl dihydrogen phosphate (9) as a crystalline white solid.

EXAMPLE 15G

Préparation of N-[l -(chloromethyl)-2-oxo-4-pyridyl]-2-(4-fluoro-2-methyl-phenoxy)-4(trifluoromethyl)benzamide (21)

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F

A solution of 2-(4-fhioro-2-methoxy-phenoxy)-N-(2-oxo-lH-pyridin-4-yl)-4-(l,l,2,2,2pentafluoroethyl)benzamide (2a) (99.9 mg, 0.2115 mmol) and chloromethyl chloroformate (32.73 mg, 22.04 pL, 0.2538 mmol) in DCM (900 pL) and DMF (100 pL) was stirred at room température for 4 hours (gas evolved). The reaction mixture was diluted with EtOAc, the organic phase washed with sat. aq. NaHCO₃, brine, dried with Na₂SO₄ and evaporated to dryness. The residue was taken up in DMF (999 pL), di-terA butoxyphosphoryloxypotassium (105.0 mg, 0.4230 mmol) and tetrabutylammonium iodide (7.81 mg, 0.021 mmol) were added and the reaction mixture was stirred at 70 °C for 4 hours. The reaction mixture was cooled, diluted with water and extracted with EtOAc (3x). The organics were combined, washed with water then brine, dried with Na₂SO₄ and evaporated to dryness. Purification by column chromatography (4 g silica; 0 - 100% EtOAc in hexanes) gave di-terAbutyl [4-[[2-(4-fluoro-2-methoxy-phenoxy)-4-(l,l,2,2,2pentafluoroethyl)benzoyl]amino]-2-oxo-l-pyridyl]methyl phosphate (21) (35 mg, 0.05039 mmol, 23.8%) as a clear oil. ESI-MS m/z cale. 694.18787, found 695.4 (M+l)⁺; Rétention time: 0.78.

EXAMPLE 15H

Préparation ofN-[l-(chloromethyl)-2-oxo-4-pyridyl]-2-(4-fluoro-2-methyl-phenoxy)-4(trifluoromethyl)benzamide (2)

F

A solution of ditert-butyl [4-[[2-(4-fluoro-2-methoxy-phenoxy)-4-(l,l,2,2,2pentafluoroethyl)benzoyl]amino]-2-oxo-l-pyridyl]methyl phosphate (21) (35 mg, 0.05039 mmol) in CH₃CN (700.0 pL), water (700.0 pL) and AcOH (700.0 pL) was refluxed for 1 hour then evaporated to dryness. The material was then co-evaporated with CH₃CN (3x), triturated with CH₃CN, filtered, washed with CH₃CN and

Page 77 oflOO desiccated to give [4-[[2-(4-fluoro-2-methoxy-phenoxy)-4-(l,1,2,2,2-pentafluoroethyl)benzoyl]amino]-2-oxol-pyridyl]methyl dihydrogen phosphate (2) (13 mg, 0.02210 mmol, 43.9%) as a white solid. ‘H NMR (400 MHz, DMSO-d₆) δ 10.72 (s, 1H), 7.85 (d, J = 8.0 Hz, 1H), 7.63 (d, J = 7.6 Hz, 1H), 7.51 (d, J = 8.2 Hz, 1H), 7.31 (dd, J = 8.9, 5.9 Hz, 1H), 7.16 (dd, J = 10.7,2.9 Hz, 1H), 6.94 (d, J = 1.9 Hz, 1H), 6.91 - 6.84 (m, 1H), 6.77 (s, 1H), 6.47 (dd, J = 7.6,2.2 Hz, 1H), 5.54 (d, J = 9.7 Hz, 2H), 3.74 (s, 3H) ppm. ³¹P NMR (162 MHz, DMSO-d₆- 85% H3PO4 aq. as internai standard - 0 ppm) δ -1.93 (t, J = 9.7 Hz, 1H) ppm.

EXAMPLE 151

Di-iert-butyl [4-[[2-(4-fluorophenoxy)-5-(trifluoromethyl)benzoyl] amino] -2-oxo-1 -pyridyl]methyl phosphate

A solution of 2-(4-fluorophenoxy)-N-(2-oxo-lH-pyridin-4-yl)-5-(trifluoromethyl)benzamide (18a) (98 mg, 0.2498 mmol) and chloromethyl chloroformate (38.66 mg, 26.39 pL, 0.2998 mmol) in CH2CI2 (1 mL) and DMF (100 pL) was stirred at room température for 4 hours (gas evolved). The reaction mixture was diluted with EtOAc and the organic phase washed with sat. aq. NaHCO₃, brine, dried with Na₂SO₄ and evaporated to dryness. The residue was taken up in DMF (1 mL), di-ier/-butoxyphosphoryloxypotassium (124.0 mg, 0.4996 mmol) and tetrabutylammonium iodide (9.227 mg, 0.02498 mmol) were added and the reaction mixture was stirred at 70 °C for 4 hours. The reaction mixture was cooled, diluted with water and extracted with EtOAc (3x). The organic layers were combined, washed with water then brine, dried with Na₂SO₄ and evaporated to dryness. Purification by column chromatography (4 g silica; 0 - 100% EtOAc in Hx) gave ditert-butyl [4-[[2-(4-fhjorophenoxy)-5-(trifluoromethyl)benzoyl]amino]-2-oxo-l-pyridyl]methyl phosphate (22) (67 mg, 0.1090 mmol, 43.7%) as a clear oil. ESI-MS m/z cale. 614.1805, found 615.5 (M+l)+; Rétention time: 0.73 minutes.

EXAMPLE 15 J [4-[[2-(4-Fluorophenoxy)-5-(trifluoromethyl)benzoyl]amino]-2-oxo-l-pyridyl]methyl dihydrogen phosphate (18)

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A solution of di-Zeri-butyl [4-[[2-(4-fhiorophenoxy)-5-(trifluoromethyl)benzoyl]amino]-2-oxo-lpyridyl]methyl phosphate (22) (67 mg, 0.1090 mmol) in CH₃CN (1.340 mL), water (1.340 mL) and AcOH (1.340 mL) was refluxed for 1 hour then evaporated to dryness. The material was then co-evaporated with CH₃CN (3x), triturated with CH₃CN, filtered, washed with CH₃CN and desiccated to give [4-[[2-(4fhiorophenoxy)-5-(trifluoromethyl)benzoyl]amino]-2-oxo-l-pyridyl]methyl dihydrogen phosphate (18) (32 mg, 0.06052 mmol, 55.5%) as a white solid. ESI-MS m/z cale. 502.0553, found 503.4 (M+l)+; Rétention time: 1.39 minutes. Ή NMR (400 MHz, DMSO-d₆) δ 10.70 (s, 1H), 8.00 (d, J = 2.4 Hz, 1H), 7.83 (dd, J = 8.8,2.4 Hz, 1H), 7.61 (d, J = 7.6 Hz, 1H), 7.35 - 7.23 (m, 4H), 7.00 (d, J = 8.7 Hz, 1H), 6.91 (d, J = 2.2 Hz, 1H), 6.45 (dd, J = 7.6, 2.3 Hz, 1H), 5.53 (d, J = 9.7 Hz, 2H) ppm. ³¹P NMR (162 MHz, DMSO-d₆- 85% H₃PO₄ aq. as internai standard - 0 ppm) δ -2.11 (t, J = 9.6 Hz) ppm.

EXAMPLE 15K

Di-ieri-butyl [4-[[4-chloro-2-(4-fluoro-2-methyl-phenoxy)benzoyl]amino]-2-oxo-l-pyridyl]methyl phosphate

A solution of 4-chloro-2-(4-fluoro-2-methyl-phenoxy)-N-(2-oxo-lH-pyridin-4-yl)benzamide (13a) (99 mg, 0.2656 mmol) and chloromethyl chloroformate (82.19 mg, 55.35 pL, 0.6374 mmol) in THF (2 mL) was added DMF (0.2 mL) and CH2CI2 (0.5 mL) and was stirred at room température for 2 hours. The reaction mixture was diluted with EtOAc and the organic phase washed with sat. aq. NaHCO₃, brine, dried with Na₂SO₄ and evaporated to dryness. The residue was taken up in DMF (1 mL), di-tertbutoxyphosphoryloxypotassium (263.7 mg, 1.062 mmol) and tetrabutylammonium iodide (9.810 mg, 0.02656 mmol) were added and the reaction mixture was stirred at 70 °C for 4 hours. The reaction mixture

Page 79 of 100 was cooled, diluted with water and extracted with EtOAc (3x). The organic phases were combined, washed with water then brine, dried with Na₂SO₄ and evaporated to dryness. Purification by column chromatography (12 g silica; 0 -100% EtOAc in Hx) gave di-ieri-butyl [4-[[4-chloro-2-(4-fluoro-2-methylphenoxy)benzoyl]amino]-2-oxo-l-pyridyl]methyl phosphate (23) (35 mg, 0.05882 mmol, 22.2%) as a clear foam. ESI-MS m/z cale. 594.1698, found 595.5 (M+l)+; Rétention time: 0.77 minutes.

EXAMPLE 15L [4-[[4-Chloro-2-(4-fluoro-2-methyl-phenoxy)benzoyl]amino]-2-oxo-l-pyridyl]methyl dihydrogen phosphate (13)

O

Cl

A solution of di-ieri-butyl [4-[[4-chloro-2-(4-fluoro-2-methyl-phenoxy)benzoyl]amino]-2-oxo-lpyridyljmethyl phosphate (23) (35 mg, 0.05882 mmol) in CH₃CN (700.0 pL), water (700.0 pL) and AcOH (0.7 mL, 12.31 mmol) was heated at 90 °C for 20 min then evaporated and co-evaporated with CH₃CN (3x). The material was triturated with CH₃CN, filtered, washed with CH₃CN and desiccated to give [4-[[4-chloro2-(4-fluoro-2-methyl-phenoxy)benzoyl]amino]-2-oxo-l-pyridyl]methyl dihydrogen phosphate (13) (13 mg, 0.02558 mmol, 43.5%) as a white solid. ESI-MS m/z cale. 482.0446, found 483.4 (M+l)+; Rétention time: 1.41 minutes. *H NMR (400 MHz, DMSO-d₆) δ 10.59 (s, 1H), 7.66 (d, J = 8.2 Hz, 1H), 7.60 (d, J = 7.5 Hz, 1H), 7.30 (dd, J = 8.2, 2.0 Hz, 1H), 7.21 (d, J = 9.3 Hz, 1H), 7.16 - 7.05 (m, 2H), 6.89 (d, J = 2.3 Hz, 1H), 6.74 (d, J = 2.0 Hz, 1H), 6.44 (dd, J = 7.6, 2.3 Hz, 1H), 5.51 (d, J = 9.7 Hz, 2H), 2.16 (s, 3H) ppm. ³¹P NMR (162 MHz, DMSO-d₆- 85% H3PO4 aq. as internai standard - 0 ppm) δ -2.15 (t, J = 9.7 Hz) ppm.

EXAMPLE 15M

Di-ieri-butyl [4-[[2-(4-fluoro-2-methyl-phenoxy)-5-(trifluoromethyl)benzoyl]amino]-2-oxo-l-pyridyl]methyl phosphate (24)

Page 80 oflOO

To a solution of 2-(4-fluoro-2-methyl-phenoxy)-N-(2-oxo-lH-pyridin-4-yl)-5-(trifluoromethyl)benzamide (10a) (102 mg, 0.2510 mmol) and chloromethyl chloroformate (77.67 mg, 52.30 pL, 0.6024 mmol) in CH₂C1₂ (2 mL) was added DMF (200 pL) and the reaction mixture was stirred at room température for 1 hour. At this time, more chloromethyl chloroformate (77.67 mg, 52.30 pL, 0.6024 mmol) was added and the reaction mixture was heated to 70 °C for 25 min. The reaction mixture was diluted with EtOAc, the organic phase washed with sat. aq. NaHCO₃, brine, dried with Na₂SO₄ and evaporated to dryness. The residue was taken up in DMF (3 mL), di-teri-butoxyphosphoryloxypotassium (249.3 mg, 1.004 mmol) and tetrabutylammonium iodide (9.271 mg, 0.02510 mmol) were added and the reaction mixture was stirred at 70 °C for 4 hours. The reaction mixture was cooled, diluted with water and extracted with EtOAc (3x). The organic layers were combined, washed with water then brine, dried with Na₂SO₄ and evaporated to dryness. Purification by column chromatography (12 g silica; 0 - 100% EtOAc in Hx) gave di-teri-butyl [4-[[2-(4fluoro-2-methyl-phenoxy)-5-(trifluoromethyl)benzoyl]amino]-2-oxo-l-pyridyl]methyl phosphate (24) (23 mg, 0.03659 mmol, 14.6%) as a clear glass. ESI-MS m/z cale. 628.19617, found 629.5 (M+l)+; Rétention time: 0.78 minutes

EXAMPLE 15N [4-[[2-(4-Fluoro-2-methyl-phenoxy)-5-(trifluoromethyl)benzoyl]amino]-2-oxo-l-pyridyl]methyl dihydrogen

A solution of di-terPbutyl [4-[[2-(4-fluoro-2-methyl-phenoxy)-5-(trifluoromethyl)benzoyl]amino]-2-oxo-lpyridyljmethyl phosphate (24) (23 mg, 0.03659 mmol) in CH₃CN (460.0 pL), water (460.0 pL) and AcOH (460.0 pL) was heated at 90°C for 15 min then evaporated and co-evaporated with CH₃CN (3x). The

Page 81 of 100 material was triturated with CH₃CN, filtered, washed with CH₃CN and desiccated to give [4-[[2-(4-fluoro-2methyl-phenoxy)-5-(trifluoromethyl)benzoyl]amino]-2-oxo-l-pyridyl]methyl dihydrogen phosphate (10) (6 mg, 0.01104 mmol, 30.2%) as a white solid. ESI-MS m/z cale. 516.0709, found 517.4 (M+l)+; Rétention time: 1.45 minutes. Ή NMR (400 MHz, DMSO-d₆) δ 11.14 (s, 1H), 8.42 (d, J = 2.4 Hz, 1H), 8.22 (dd, J = 8.8,2.2 Hz, 1H), 8.05 (d, J = 7.6 Hz, 1H), 7.67 (dd, J = 9.5, 3.1 Hz, 1H), 7.65 - 7.50 (m, 2H), 7.35 (d, J = 2.3 Hz, 1H), 7.26 (d, J = 8.7 Hz, 1H), 6.88 (dd, J = 7.6,2.4 Hz, 1H), 5.95 (d, J = 9.8 Hz, 2H), 2.57 (s, 3H) ppm. ^3IP NMR (162 MHz, DMSO-dô- 85% H₃PO₄ aq. as internai standard - 0 ppm) δ -2.17 (t, J = 9.7 Hz) ppm.

EXAMPLE 150

Di-tert-butyl [4-[[4,5-dichloro-2-(4-fluoro-2-methoxy-phenoxy)benzoyl]amino]-2-oxo-l-pyridyl]methyl phosphate (25)

Cl

O

Cl

To a solution of 4,5-dichloro-2-(4-fluoro-2-methoxy-phenoxy)-N-(2-oxo-lH-pyridin-4-yl)benzamide (la) (101 mg, 0.2386 mmol) and chloromethyl chloroformate (36.92 mg, 24.86 pL, 0.2863 mmol) in CH2CI2 (2 mL) was added DMF (200 pL) and the reaction mixture was stirred at room température for 1 hour. At this time, THF (1 mL) was added followed by more chloromethyl chloroformate (36.92 mg, 24.86 pL, 0.2863 mmol) and the reaction mixture stirred at room température for 1 h. More DMF (1 mL) and chloromethyl chloroformate (36.92 mg, 24.86 pL, 0.2863 mmol) was added and the reaction mixture was heated to 70 °C for 15 min. The reaction mixture was diluted with EtOAc and the organic phase washed with sat. aq. NaHCO₃, brine, dried with Na₂SO₄ and evaporated to dryness. The residue was taken up in DMF (1.010 mL), di-terAbutoxyphosphoryloxypotassium (118.5 mg, 0.4772 mmol) and tetrabutylammonium iodide (8.813 mg, 0.02386 mmol) was added and the reaction mixture stirred at 70 °C for 4 h. The reaction mixture was cooled, diluted with water and extracted with EtOAc (3x). The organic layers were combined, washed with water then brine, dried with Na₂SO₄ and evaporated to dryness. Purification by column chromatography (12 g silica; 0 -100% EtOAc in Hx) gave di-tert-butyl [4-[[4,5-dichloro-2-(4-fluoro-2methoxy-phenoxy)benzoyl]amino]-2-oxo-l-pyridyl]methyl phosphate (25) (40 mg, 0.06197 mmol, 26.0%) as a clear glass. ESI-MS m/z cale. 644.12573, found 647.3 (M+l)+; Rétention time: 0.81 minutes

EXAMPLE 15P

Page 82 of 100 [4-[[4,5-Dichloro-2-(4-fluoro-2-methoxy-phenoxy)benzoyl]amino]-2-oxo-l-pyridyl]methyl dihydrogen phosphate (1)

A solution of di-teri-butyl [4-[[4,5-dichloro-2-(4-fluoro-2-methoxy-phenoxy)benzoyl]amino]-2-oxo-lpyridyljmethyl phosphate (40 mg, 0.06197 mmol) in CH₃CN (800.0 pL), water (800.0 pL) and AcOH (800 pL, 14.07 mmol) was heated at 90°C for 15 min then evaporated and co-evaporated with CH₃CN (3x). The material was triturated with CH₃CN, filtered, washed with CH₃CN and desiccated to give [4-[[4,5-dichloro2-(4-fluoro-2-methoxy-phenoxy)benzoyl]amino]-2-oxo-l-pyridyl]methyl dihydrogen phosphate (1) (19 mg, 0.03385 mmol, 54.6%) as a white solid. ESI-MS m/z cale. 532.00055, found 533.3 (M+l)+; Rétention time: 1.5 minutes. 'H NMR (400 MHz, DMSO-d₆) δ 10.58 (s, 1H), 7.91 (s, 1H), 7.62 (d, J = 7.6 Hz, 1H), 7.27 (dd, J = 8.9, 5.8 Hz, 1H), 7.13 (dd, J= 10.7, 3.0 Hz, 1H), 6.90 (d, J = 2.3 Hz, 1H), 6.86 (s, 1H), 6.86 - 6.81 (m, 1H), 6.44 (dd, J = 7.6, 2.4 Hz, 1H), 5.52 (d, J = 9.7 Hz, 2H), 3.76 (s, 3H) ppm. ³¹P NMR (162 MHz, DMSOd₆-85% H₃PO₄ aq. as internai standard - 0 ppm) δ -2.12 (t, J = 9.4 Hz) ppm.

Compounds 3-8, 11,12 and 14-17 may be prepared using similar procedures as described above for preparing compounds 1, 2, 9,10,13 and 18.

EXAMPLE 15Q

A spray dry dispersion of compound 9 with 50% HPMCAS was prepared as follows. Compound 9 (14 grams) was added to a beaker (1000ml), followed by 400 mL of THF/H₂0 (95:5) and 100 mL of MeOH. The material was stirred for 1 h giving a slightly hazy solution. In a separate container, 14 grams of Hydroxypropylmethylcellulose acetate succinate HF grade (HPMCAS-HF) was dissolved in 100 mL of THF and the mixture was stirred for 15 minutes. The two solutions were combined and stirred continuously while spray drying using a Buchi Mini Spray Dryer with the following parameters:

T inlet (setpoint) 78°C

T outlet (start) 38°C

T outlet (end) 35°C

Page 83 of 100

Nitrogen Pressure

Aspirator

Pump

Rotometer

Filter Pressure

Condenser Temp psi

100 %

20% mm

-50 mbar

-5 °C

Run Time

Approximately 18.2 g of compound 9 amorphous Form C as a spray dry dispersion (65% yield) was recovered. The amorphous Form C of compound 9 in the spray dry dispersion was confirmed by XRPD (Figure 5) and DSC, showing a glass transition température of 96 °C.

A neat amorphous Form C of compound 9 was prepared by spray dry dispersion according to the conditions discussed above except that no HPMCAS polymer was added. The neat amorphous Form C of compound 9 in the spray dry dispersion was confirmed by XRPD (Figure 6).

The structure of crystalline Form B of compound 9 was confirmed by single-crystal x-ray diffraction analysis (Figure 1). Single crystal diffraction data was acquired on a Broker Apex H diffractometer equipped with sealed tube Cu K-alpha source (Cu Ko. radiation, γ = 1.54178 Â) and an Apex Π CCD detector. A colorless plate shaped crystal with dimensions of 0.01 x 0.05 x 0.05 mm was selected for data collection. Three batches of 40 fiâmes separated in reciprocal space were obtained to provide an orientation matrix and initial cell parameters. Final cell parameters were obtained and refined after data collection was completed based on the full data set.

A diffraction data set of reciprocal space was obtained to a resolution of 0.84 Â using 1.0° steps using 60 seconds exposures for each low angle frame and 120 seconds for each high angle frame. Observation of the crystal after data collection showed no signs of décomposition.

The data was collected, refined and reduced using the Broker Apex software. The structure was solved using the SHELXS97 (Sheldrick, 1990); program(s) and the structure refined using the SHELXL97 (Sheldrick, 1997) program. The crystal shows monoclinic cell with P2JC space group. The lattice parameters are a = 20.194(9)Â, b = 9.205(4)Â, c = 11.956(5)Â, β = 95.213(8)°. Volume = 2213.4(17)Â³. The high angle reflections were weak, leading to a high R factor 9.8%. However, the structure was ordered and there was one symmetry independent molécule in the structure.

Table 3. Analytical Data for Compounds and Intermediates

Page 84 of 100

Cmpd. No.	LCMS Ret. Time in minutes	MS (M+l)	’H-NMR (400 MHz)
1	1.50	533.3	(DMSO-d₆) δ 10.58 (s, 1H), 7.91 (s, 1H), 7.62 (d, J = 7.6 Hz, 1H), 7.27 (dd, J = 8.9, 5.8 Hz, 1H), 7.13 (dd, J = 10.7, 3.0 Hz, 1H), 6.90 (d, J = 2.3 Hz, 1H), 6.86 (s, 1H), 6.86 - 6.81 (m, 1H), 6.44 (dd, J = 7.6, 2.4 Hz, 1H), 5.52 (d, J = 9.7 Hz, 2H), 3.76 (s, 3H) PPm
la	1.57	423.3	(DMSO-d₆) δ 11.27 (s, 1H), 10.49 (s, 1H), 7.90 (br s, 1H), 7.32 (d, J = 7.2 Hz, 1H), 7.28 (dd, J = 8.9, 5.8 Hz, 1H), 7.14 (dd, J = 10.7, 2.9 Hz, 1H), 6.89 - 6.82 (m, 2H), 6.77 (s, 1H), 6.39 (dd, J = 7.2, 2.1Hz, 1H), 3.76 (s, 3H)ppm
2	1.54	583.4	(DMSO-d₆) δ 10.72 (s, 1H), 7.85 (d, J = 8.0 Hz, 1H), 7.63 (d, J = 7.6 Hz, 1H), 7.51 (d, J = 8.2 Hz, 1H), 7.31 (dd, J = 8.9, 5.9 Hz, 1H), 7.16 (dd, J = 10.7, 2.9 Hz, 1H), 6.94 (d, J = 1.9 Hz, 1H), 6.91 - 6.84 (m, 1H), 6.77 (s, 1H), 6.47 (dd, J = 7.6, 2.2 Hz, 1H), 5.54 (d, J = 9.7 Hz, 2H), 3.74 (s, 3H) ppm
2a	1.62	473.3	(DMSO-d₆) δ 11.32 (s, 1H), 10.62 (s, 1H), 7.83 (d, J = 7.9 Hz, 1H), 7.51 (d, J = 7.9 Hz, 1H), 7.39 - 7.27 (m, 2H), 7.17 (dd, J = 10.7, 2.8 Hz, 1H), 6.88 (dd, J = 11.3, 5.7 Hz, 1H), 6.81 (s, 1H), 6.75 (s, 1H), 6.43 (d, J = 7.1 Hz, 1H), 3.73 (s, 3H) ppm
3a	1.53	393.1	(DMSO-d₆) δ 11.32 (s, 1H), 10.57 (s, 1H), 7.95 (s, 1H), 7.34 - 7.24 (m, 3H), 7.22 - 7.15 (m, 3H), 6.74 (s, 1H), 6.38 (d, J = 7.2 Hz, 1H) ppm
4a	1.56	423.2
5a	1.8	459.5
6a	1.59	443.5	(DMSO-d₆) δ 7.87 (d, J = 7.9 Hz, 1H), 7.59 (d, J = 7.6 Hz, 1H), 7.37 - 7.25 (m, 3H), 7.24 - 7.16 (m, 2H), 7.07 (s, 1H), 6.73 (s, 1H), 6.37 (d, J = 6.9 Hz, lH)ppm
7a	1.52	389.1
8a	1.93	459.3	(DMSO-d₆) δ 11.29 (s, 1H), 10.62 (s, 1H), 8.02 (d, J = 2.3 Hz, 1H), 7.86 (dd, J = 8.7, 2.4 Hz, 1H), 7.58 - 7.40 (m, 2H), 7.40 7.21 (m, 3H), 7.11 (d, J = 8.7 Hz, 1H), 6.76 (d, J = 2.1 Hz, 1H), 6.38 (dd, J = 7.2,2.1 Hz, 1H) ppm
9	1.79	407.1	(DMSO-d₆) δ 11.27 (s, 1H), 10.63 (s, 1H), 7.84 (d, J = 7.8 Hz, 1H), 7.60 (d, J = 7.8 Hz, 1H), 7.31 (d, J = 7.2 Hz, 1H), 7.23 (m, 1H), 7.10 (m, 2H), 6.97 (s, 1H), 6.76 (d, J = 1.6 Hz, 1H), 6.38 (dd, J = 7.2,2.0 Hz, 1H), 2.16 (s, 3H) ppm
9a	1.79	407.1	(DMSO-d₆) δ 11.27 (s, 1H), 10.63 (s, 1H), 7.84 (d, J = 7.8 Hz, 1H), 7.60 (d, J = 7.8 Hz, 1H), 7.31 (d, J = 7.2 Hz, 1H), 7.23 (m, 1H), 7.10 (m, 2H), 6.97 (s, 1H), 6.76 (d, J = 1.6 Hz, 1H), 6.38 (dd, J = 7.2,2.0 Hz, 1H), 2.16 (s, 3H) ppm
10	1.45	517.4	(DMSO-de) δ 11.14 (s, 1H), 8.42 (d, J = 2.4 Hz, 1H), 8.22 (dd, J = 8.8, 2.2 Hz, 1H), 8.05 (d, J = 7.6 Hz, 1H), 7.67 (dd, J = 9.5, 3.1 Hz, 1H), 7.65 - 7.50 (m, 2H), 7.35 (d, J = 2.3 Hz, 1H), 7.26 (d, J = 8.7 Hz, 1H), 6.88 (dd, J = 7.6, 2.4 Hz, 1H), 5.95 (d, J = 9.8 Hz, 2H), 2.57 (s, 3H) ppm
10a	1.6	407.2	(DMSO-d₆) δ 11.77 (s, 1H), 10.79 (s, 1H), 8.00 (d, J = 2.2 Hz, 1H), 7.80 (dd, J = 8.8, 2.3 Hz, 1H), 7.45 (d, J = 7.2 Hz, 1H), 7.25 (dd, J = 9.3, 3.0 Hz, 1H), 7.16 (m, 2H), 6.95 (d, J = 1.9 Hz, 1H),

Page 85 of 100

Cmpd. No.	LCMS Ret. Time in minutes	MS (M+l)	Ή-NMR (400 MHz)
			6.83 (d, J = 8.7 Hz, 1H), 6.56 (dd, J = 7.2, 2.0 Hz, 1H), 2.14 (s, 3H) ppm
lia	1.57	427.2	(DMSO-d_ô) δ 11.29 (s, 1H), 10.62 (s, 1H), 8.02 (d, J = 2.2 Hz, 1H), 7.83 (dd, J = 8.8, 2.2 Hz, 1H), 7.69 (dd, J = 8.4, 3.0 Hz, 1H), 7.45 (dd, J = 9.1, 5.3 Hz, 1H), 7.40 - 7.30 (m, 2H), 6.92 (d, J = 8.7 Hz, 1H), 6.79 (d, J = 1.9 Hz, 1H), 6.42 (dd, J = 7.2, 2.1 Hz, 1H) ppm
12a	1.57	373.1
13	1.41	483.4	(DMSO-dô) δ 10.59 (s, 1H), 7.66 (d, J = 8.2 Hz, 1H), 7.60 (d, J = 7.5 Hz, 1H), 7.30 (dd, J = 8.2, 2.0 Hz, 1H), 7.21 (d, J = 9.3 Hz, 1H), 7.16 - 7.05 (m, 2H), 6.89 (d, J = 2.3 Hz, 1H), 6.74 (d, J = 2.0 Hz, 1H), 6.44 (dd, J = 7.6, 2.3 Hz, 1H), 5.51 (d, J = 9.7 Hz, 2H), 2.16 (s, 3H) ppm.
13a	1.57	373.2	(DMSO-d₆) δ 11.48 (s, 1H), 10.57 (s, 1H), 7.65 (d, J = 8.2 Hz, 1H), 7.36 (d, J = 7.2 Hz, 1H), 7.30 (dd, J = 8.2, 1.9 Hz, 1H), 7.21 (d, J = 9.3 Hz, 1H), 7.10 (m, 2H), 6.83 (d, J = 1.9 Hz, 1H), 6.74 (d, J= 1.9 Hz, 1H), 6.45 (dd, J = 7.2, 2.1 Hz, 1H), 2.16 (s, 3H) ppm
14a	1.64	393.1
15a	1.48	359.2	(DMSO-dô) δ 11.25 (s, 1H), 10.48 (s, 1H), 7.65 (t, J = 8.5 Hz, 1H), 7.29 (m, 4H), 7.19 (m, 2H), 6.91 (d, J = 1.9 Hz, 1H), 6.75 (d, J = 1.9 Hz, 1H), 6.38 (dd, J = 7.2, 2.1 Hz, 1H) ppm
16a	1.55	411.17
17a	1.83	459.3	(DMSO-d₆) δ 11.28 (s, 1H), 10.63 (s, 1H), 8.04 (d, J = 2.3 Hz, 1H), 7.86 (dd, J = 8.7, 2.4 Hz, 1H), 7.60 - 7.54 (m, 1H), 7.54 7.43 (m, 1H), 7.43 - 7.34 (m, 1H), 7.34 - 7.28 (m, 2H), 7.05 (d, J = 8.7 Hz, 1H), 6.75 (d, J = 2.0 Hz, 1H), 6.38 (dd, J = 7.2, 2.1 Hz, 1H) ppm
18	1.39	503.4	(DMSO-d₆) δ 10.70 (s, 1H), 8.00 (d, J = 2.4 Hz, 1H), 7.83 (dd, J = 8.8, 2.4 Hz, 1H), 7.61 (d, J = 7.6 Hz, 1H), 7.35 - 7.23 (m, 4H), 7.00 (d, J = 8.7 Hz, 1H), 6.91 (d, J = 2.2 Hz, 1H), 6.45 (dd, J = 7.6,2.3 Hz, 1H), 5.53 (d, J = 9.7 Hz, 2H) ppm
18a	1.72	393.1	(DMSO-d₆) δ 11.40 (s, 1H), 10.64 (s, 1H), 8.00 (d, J = 2.2 Hz, 1H), 7.83 (dd, J = 8.8, 2.3 Hz, 1H), 7.30 (tdd, J = 6.9, 5.9, 3.4 Hz, 5H), 6.99 (d, J = 8.8 Hz, 1H), 6.82 (d, J = 1.8 Hz, 1H), 6.43 (dd, J = 7.2, 2.0 Hz, 1H) ppm

EXAMPLE 16

ASSAYS FOR DETECTINGAND MEASURING Nay INHIBITION PROPERTIES OF COMPOUNDS

E-VIPR optical membrane potential assay method with electrical stimulation

Sodium channels are voltage-dependent proteins that can be activated by inducing membrane voltage changes by applying electric fîelds. The electrical stimulation instrument and methods of use are described

Page 86 of 100 in Ion Channel Assay Methods PCT/US01/21652, herein incorporated by reference and are referred to as EVIPR. The instrument comprises a microtiter plate handler, an optical System for exciting the coumarin dye while simultaneously recording the coumarin and oxonol émissions, a waveform generator, a current- or voltage-controlled amplifier, and a device for inserting électrodes in well. Under integrated computer control, this instrument passes user-programmed electrical stimulus protocols to cells within the wells of the microtiter plate. The E-VIPR assay is conducted according to the foilowing procedure (including reagents and solutions, assay protocol, and data analysis). For simplicity, the procedure is described in the past tense, but it will be understood that this procedure applies to such assays conducted in the past (if any) and to such assays conducted in the future (if any).

hours before the assay on E-VIPR, HEK cells expressing human Navl.8 were seeded in 384-well polylysine coated plates at 15,000-20,000 cells per well. HEK cells were grown in media (exact composition is spécifie to each cell type and NaV subtype) supplemented with 10% FBS (Fêtai Bovine Sérum, qualifïed; GibcoBRL #16140-071) and 1% Pen-Strep (Penicillin-Streptomycin; GibcoBRL #15140-122). Cells were grown in vented cap flasks, in 90% humidity and 5% CO₂.

Reagents and Solutions:

100 mg/mL Pluronic F-127 (Sigma #P2443), in dry DMSO

Compound Plates: 384-well round bottom plate, e.g. Coming 384-well Polypropylene Round Bottom #3656

Cell Plates: 384-well tissue culture treated plate, e.g. Greiner #781O91-1B mM DiSBAC₆(3) (Aurora #00-100-010) in dry DMSO mM CC2-DMPE (Aurora #00-100-008) in dry DMSO

200 mM ABSC1 in H₂O

Bathl buffer: Glucose lOmM (1.8g/L), Magnésium Chloride (Anhydrous), ImM (0.095g/L), Calcium Chloride, 2mM (0.222g/L), HEPES lOmM (2.38g/L), Potassium Chloride, 4.5mM (0.335g/L), Sodium Chloride 160mM (9.35g/L).

Hexyl Dye Solution: Bathl Buffer + 0.5% β-cyclodextrin (made this prior to use, Sigma #C4767), 8 μΜ CC2-DMPE + 2.5 μΜ DiSBAC₆(3). To make the solution added volume of 10% Pluronic F127 stock equal to volumes of CC2-DMPE + DiSBAC₆(3). The order of préparation was first mixing Pluronic and CC2DMPE, then adding DiSBAC_â(3) while vortexing, then adding Bathl + β-Cyclodextrin.

Assay Protocol:

1) Pre-spotted compounds (in neat DMSO) into compound plates. Vehicle control (neat DMSO), the positive control (20mM DMSO stock tetracaine, 125 μΜ final in assay) and test compounds were added to Page 87 oflOO each well at 160x desired final concentration in neat DMSO. Final compound plate volume was 80 pL (80fold intermediate dilution from 1 pL DMSO spot; 160-fold final dilution after transfer to cell plate). Final DMSO concentration for ail wells in assay was 0.625%.

2) Prepared Hexyl Dye Solution.

3) Prepared cell plates. On the day of the assay, medium was aspirated and cells were washed three times with 100 pL of Bathl Solution, maintaining 25 pL residual volume in each well.

4) Dispensed 25 pL per well of Hexyl Dye Solution into cell plates. Incubated for 20-35 minutes at room temp or ambient conditions.

5) Dispensed 80 pL per well of Bathl into compound plates. Acid Yellow-17 (1 mM) was added and Potassium Chloride was altered from 4.5 to 20 mM depending on the NaV subtype and assay sensitivity.

6) Washed cell plates three times with 100 pL per well of Bathl, leaving 25 pL of residual volume. Then transfered 25uL per well from Compound Plates to Cell Plates. Incubated for 20-35 minutes at room temp/ambient condition.

7) Read Plate on E-VIPR. Used the current-controlled amplifier to deliver stimulation wave puises for 10 seconds and a scan rate of 200Hz. A pre-stimulus recording was performed for 0.5 seconds to obtain the unstimulated intensifies baseline. The stimulatory waveform was followed by 0.5 seconds of post-stimulation recording to examine the relaxation to the resting state.

Data Analysis

Data was analyzed and reported as normalized ratios of émission intensifies measured in the 460 nm and 580 nm channels. The response as a function of time was reported as the ratios obtained using the following formula::

(intensity 4ûo nm -background_460nm)

R_(t)= --------------------------------------------(intensity ₅₈₀ „m - background ₅₈₀ J

The data was further reduced by calculating the initial (R) and final (R_f) ratios. These were the average ratio values during part or ail of the pre-stimulation period, and during sample points during the stimulation period. The response to the stimulus RD □= Rf/Ri was then calculated and reported as a function of time.

Control responses were obtained by performing assays in the presence of a compound with the desired properties (positive control), such as tetracaine, and in the absence of pharmacological agents (négative control). Responses to the négative (TV) and positive (P) controls were calculated as above. The compound antagonist activity A is defined as:

Page 88 oflOO

R-P

A = ———*100 .

N ~ P where R is the ratio response of the test compound

EXAMPLE 17

ELECTROPHYSIOLOGY ASSAYS FOR Nay ACTIVITY AND INHIBITION OF TEST COMPOUNDS

Patch clamp electrophysiology was used to assess the efficacy and selectivity of sodium channel blockers in dorsal root ganglion neurons. Rat neurons were isolated from the dorsal root ganglions and maintained in culture for 2 to 10 days in the presence of NGF (50 ng/ml) (culture media consisted of NeurobasalA supplemented with B27, glutamine and antibiotics). Small diameter neurons (nociceptors, 8-12 pm in diameter) were visually identifïed and probed with fine tip glass électrodes connected to an amplifier (Axon Instruments). The “voltage clamp” mode was used to assess the compound’s IC₅₀ holding the cells at - 60 mV. In addition, the “current clamp” mode was employed to test the efficacy of the compounds in blocking action potential génération in response to current injections. The results of these experiments contributed to the définition of the efficacy profile of the compounds.

Selected compounds and intermediates of the présent invention herein are active against Na_v1.8 sodium channels as measured using the assays described herein and as presented in Table 4 below.

Table 4. Na_v 1.8 IC₅q activity

Cmpd. No	Na_v1.8 IC₅₀(μΜ)
1	0.004
la	0.001
2	0.028
2a	0.003
3a	0.006
4a	0.011
5a	0.011
6a	0.012
7a	0.013
8a	0.013
9	0.33
9a	0.014
10	0.086
10a	0.017
lia	0.028
12a	0.03
13	0.329
13a	0.03
14a	0.037
15a	0.044
16a	0.05

Page 89 oflOO

Cmpd. No	Na_v1.8 ICso (μΜ)
17a	0.051
18	0.457
18a	0.054

EXAMPLE 18

IonWorks assays. This assay is performed to détermine the activity for the compounds of the présent invention against non Na_vl .8 channels. This assay is conducted according to the following procedure. For simplicity, the procedure is described in the past tense, but it will be understood that this procedure applies to such assays conducted in the past (if any) and to such assays conducted in the future (if any). Sodium currents were recorded using the automated patch clamp System, IonWorks (Molecular Devices Corporation, Inc.). Cells expressing Na_v subtypes were harvested from tissue culture and placed in suspension at 0.5-4 million cells per mL Bathl. The IonWorks instrument measured changes in sodium currents in response to applied voltage clamp similarly to the traditional patch clamp assay, except in a 384-well format. Using the IonWorks, dose-response relationships were detennined in voltage clamp mode by depolarizing the cell from the experiment spécifie holding potential to a test potential of about 0 mV before and following addition of the test compound. The influence of the compound on currents were measured at the test potential.

EXAMPLE 19

AQUEOUS SOLUBILITY STUDY

The aqueous solubility of compound 9 and 9a were determined according to the following procedure.

Solubility data was determined at ambient conditions by equilibrating the compound with water on a shaking bed for 24 hours, followed by centrifugation and séparation of the saturated solutions. The pH value of each media was measured before centrifugation, and the saturated solutions were assayed by HPLC. The aqueous solubility of compound 9a in water was low (<0.001 mg/ml at pH 6.0) whereas the aqueous solubility of compound 9 in water was approximately 0.3 mg/ml.

Table 5. Aqueous Solubility of Compounds 9 and 9a:

Compound	Solid form	pH	Solubility (mg/mL)
9a	crystalline	6.0	<0.001
9a	crystalline	1.2	<0.001
9	crystalline	1	0.002
9	crystalline	3	0.11
9	crystalline	3.3	0.24
9	crystalline	5	0.42
9	crystalline	7	0.50
9	crystalline	8	2.24

Page 90 of 100

EXAMPLE 20

PHARMACOKINETIC STUDIES

The pharmacokinetic parameters of selected compounds of this invention were determined in the experiments described below. General analytic procedures and spécifie experimental protocols were employed as follows:

General Analytic Procedures

The following general analytic procedures were employed in the pharmacokinetic experiments described below:

Sample Analysis. Concentrations of compound 9 and compound 9a in plasma were determined using a high performance liquid chromatography/tandem mass spectrometry (HPLC/MS/MS) method. Before extraction, plasma samples were diluted using blank plasma at an appropriate dilution factor, as necessary, depending on the dose level. Compound 9a and compound 9 along with the internai standard (IS) were extracted from (diluted) plasma, 20 pL each, by direct protein précipitation with acetonitrile (1:25 ratio of plasma/acetonitrile). After centrifugation, the supernatant extract (10 pL) was injected onto the LC/MS/MS System. The HPLC System included a Phenomenex Synergy Kinetix C8 column, 2.6 micron, 2.0 mm diameter x 75 mm long eluted with a gradient mobile phase consisting of 0.1% formic acid in water or in acetonitrile.

The analytes were detected by MS/MS with Electrospray Ionization (ESI) in the mode of multiple reaction monitoring (MRM). The lower limit of quantitation (LLOQ) was 1 to 10 ng/mL for compound 9a and 2.5 to 25 ng/mL for compound 9. The linear range of the assay was from 1 or 10 to 10000 ng/mL for compound 9a and 2.5 or 25 to 10000 ng/mL for compound 9. The intra-day and inter-day assay accuracy was within 20% of the nominal values. The intra- and inter-day assay variability was <20%.

Pharmacokinetic Data Analysis. Plasma concentration-time profiles of compound 9a and compound 9 were analyzed by noncompartmental pharmacokinetic methods using Watson LIMS version 7.4 SP3 (Thermo Fisher Scientifïc, Inc., Philadelphia, PA)

Key pharmacokinetic parameters such as AUC_aH, AUCe_Xtra, C_max, t_inax, Cl, Vss and ty₂ were determined. Statistical Data Analysis. Descriptive statistical data of pharmacokinetic parameters were calculated, including the mean, standard déviation (SD), using Watson LIMS version 7.4 SP3 or Microsoft Excel 2000. Monkey Oral Study

Male or female cynomolgus monkeys (n=3 per dose group) were administered single nominal PO doses of 10,40,100, 300, 500, 750 and 1000 mg/kg of compound 9 by gavage. Compound 9 was formulated in 30% PEG400 and 10% TPGS in water or 5% TPGS, 5% PVP-K30 in 50 mM Citrate, pH 5. Animais were fasted overnight prior to dosing, and were fed two hours post dose. Water was available before and after dosing.

Page 91 oflOO

Blood samples (approximately 0.25 mL each) were collected via a carotid artery cathéter prior to dosing and at 0.25, 0.5, 1, 2, 4, 8, 12, 24, 48 and 72 hours post dose. Each blood sample was collected into a tube that was kept on wet ice and contained potassium EDTA as the anticoagulant. Plasma was separated and stored at approximately -70°C until analysis.

Plasma samples were analyzed using a liquid chromatography/tandem mass spectrometry (LC/MS/MS) method to détermine the concentrations of compound 9a and compound 9 with a lower limit of quantitation (LLOQ) of 1.00 to 10.0 ng/mLfor compound 9a, and 10.0 to 25.0 ng/mL for compound 9. Plasma concentration vs. time data of compound 9a was subjected to noncompartmental pharmacokinetic (PK) analysis. The results of this analysis are provided in Table 7. For compound 9, plasma concentrations were 10 below LLOQ for the majority of the samples, and no PK analysis could be performed.

Table 7. Pharmacokinetic Data from Monkey Oral Study

Nominal Dose (mg/kg)	Formulation	Analyte	AUC ₍ extra (pg-hr/niL)	C max (pg/mL)	T max (hr)	T 1/2 (hr)
10	30%PEG400/10%TPGS/60%wate r	Cmpd 9a	21.5 + 13.7	1.13 + 0.187	4.67 + 3.06	8.57 + 2.75
40	5% TPGS/5%PVPK30/50mMCitrate pH5	Cmpd 9a	62.4 ± 23.8	4.50 + 0.51	3.33 ± 1.15	9.58 + 3.91
100	5% TPGS/5%PVP- K30/50mMCitratepH5	Cmpd 9a	49.6 + 10.7	3.34 + 0.34	4.00 + 0.00	7.04 + 0.36
300	5% TPGS/5%PVPK30/50mMCitrate pH5	Cmpd 9a	93.4 ± 22.7	5.77 + 1.46	2.67 + 1.15	11.6 ±2.3
500	5% TPGS/5%PVPK30/50mMCitrate pH5	Cmpd 9a	124 ± 6.0	5.76 + 1.69	6.00 + 3.46	9.80 + 1.08
750	5% TPGS/5%PVPK30/50mMCitrate pH5	Cmpd 9a	138 + 47.3	7.49 + 3.13	6.67 + 4.62	13.1 ±3.1
1000	5% TPGS/5%PVPK30/50mMCitrate pH5	Cmpd 9a	176 ± 20.7	7.88 + 0.80	4.00 + 0.00	13.5 ±3.1

N=3 monkeys per dose level, Mean ± Standard déviation

Rat Oral Study

Groups of male and female Sprague Dawley rats (n=3 per dose group) were administered single nominal oral doses of 10,100,400, 640,1000 mg/kg (for males) and 30, 100, 300, 640,1000 mg/kg (for females) of compound 9 by gavage. Compound 9 was formulated in either 30% PEG400 and 10% TPGS in water or 5%

Page 92 oflOO

TPGS, 5% PVP-K30 in 50 mM Citrate, pH 5. Animais had free access to food and water before and after dosing. Blood samples (approximately 0.25 mL each) were collected via a carotid artery cathéter prior to dosing and at 0.25, 0.5, 1, 2, 4, 8, 12, 24,48 and 72 hours post dose. Each blood sample was collected into a tube that was kept on wet ice and contained potassium EDTA as the anticoagulant. Plasma was separated and stored at approximately -70°C until analysis.

Plasma samples were analyzed using a liquid chromatography/tandem mass spectrometry (LC/MS/MS) method to détermine the concentrations of compound 9a and compound 9 with a lower limit of quantitation (LLOQ) of 1 to 25 ng/mLfor compound 9a and 2.5 to 25 ng/mL for compound 9. Plasma concentration vs. time data of compound 9a was subjected to noncompartmental pharmacokinetic (PK) analysis. The results 10 of this analysis are provided in Table 8. For compound 9, plasma concentrations were below LLOQ for the majority of the samples, and no PK analysis could be performed.

Table 8. Pharmacokinetic Data from Rat Oral Study

Gender	Nominal Dose (mg/kg)	Formulation	Analyte	AUC extra (pg-hr/mL)	C max (pg/mL)	T max (hr)	T 1/2 (hr)
Male	10	5% TPGS/5%PVP- K30/50mMCitratepH5	Cmpd 9a	3.6 + 0.24	0.29 ± 0.05	5.33 ± 2.31	3.80 ± 0.73
100	5% TPGS/5%PVP- K30/50mMCitratepH5	Cmpd 9a	24.8 ±4.6	1.67 ± 0.19	8.00 ± 0.00	5.36 ± 2.37
400	5% TPGS/5%PVP- K30/50mMCitratepH5	Cmpd 9a	68.6 ±25.7	3.74 ± 1.25	5.67 ± 4.04	5.20 ± 1.96
640	5% TPGS/5%PVPK30/50mMCitrate pH5	Cmpd 9a	74.9 ±24.1	4.76 ± 0.98	6.00 ± 3.46	6.91 ± 1.84
1000	5% TPGS/5%PVP- K30/50mMCitratepH5	Cmpd 9a	88.5 ±41.0	4.54 ± 0.91	6.67 ± 2.31	7.95± 2.24
Females	30	5% TPGS/5%PVP- K30/50mMCitratepH5	Cmpd 9a	99.2 ±6.88	3.33 ± 0.43	6.67 ± 2.31	10.8 ± 1.60
100	5% TPGS/5%PVPK30/50mMCitrate pH5	Cmpd 9a	187 ±17.5	5.82 ± 0.58	12.0 ± 0.00	9.15 ± 0.44
300	5% TPGS/5%PVPK30/50mMCitrate pH5	Cmpd 9a	282 ± 74.2	7.90 ± 1.30	10.7 ± 2.31	13.1± 2.65
640	5% TPGS/5%PVPK30/50mMCitrate pH5	Cmpd 9a	324 ± 32.8	10.0 ± 1.45	9.33 ± 2.31	7.51 ± 1.86
1000	5% TPGS/5%PVPK30/50mMCitrate pH5	Cmpd 9a	345 ± 69.6	10.6 ± 3.58	13.3 ± 9.24	7.90 ± 3.37

N=3 rats per dose level, Mean ± Standard déviation

Rat IV Study

Male Sprague Dawley rats (n=3) were administered a single nominal IV bolus dose of 1 mg/kg of compound via a jugular vein cannula. Compound 9 was formulated in DMI vehicle, consisting of 35% PEG400,15%

Page 93 oflOO éthanol, 10% dimethyl isosorbide and 40% of (5% dextran in water). Animais had free access to food and water before and after dosing. Blood samples (approximately 0.25 mL each) were collected via a carotid artery cathéter prior to dosing and at 5min, 10min, 0.25, 0.5, 1, 2, 4, 8, 12, 24 hours post dose. Each blood sample was collected into a tube that was kept on wet ice and contained potassium EDTA as the anticoagulant. Plasma was separated and stored at approximately -70°C until analysis.

Plasma samples were analyzed using a liquid chromatography/tandem mass spectrometry (LC/MS/MS) method to détermine the concentrations of compound 9a and compound 9 with a lower limit of quantitation (LLOQ) of 1 ng/mL for compound 9a and 2.5 ng/mL for compound 9. Plasma concentration vs. time data were subjected to noncompartmental pharmacokinetic (PK) analysis. The résulte of this analysis are provided in Table 9. For compound 9, plasma concentrations were below LLOQ for the majority of the samples, and no PK analysis could be performed.

Table 9. Pharmacokinetic Data from Rat IV Study

Dose (mg/ kg)	Formulation	Analyte	CO (pg/ml)	AUC _all(pg*hr/ml)	AUC extra (pg*hr/ ml)	tl/2 (hr)	Cl (ml/min/kg)	Vss (L/kg)
1	DMI	Cmpd 9a	0.259	0.592	0.611	2.43	18.7	3.77

The studies described above, demonstrate that compound 9 is converted in vivo into compound 9a in at least rats and monkeys.

Many modifications and variations of the embodiments described herein may be made without departing from the scope, as is apparent to those skilled in the art. The spécifie embodiments described herein are offered by way of example only.

Claims

1. A compound of formula I:

wherein, independently for each occurrence:

R² and R³ are independently hydrogen, halogen, or alkyl wherein said Ci-Cô alkyl is substituted with 0-6 halogen;

R⁵ is hydrogen, halogen, OH, or Cj-Cô alkyl wherein said Ci-Cô alkyl is substituted with 0-6 halogen and wherein up to two non-adjacent CH₂ units of said Cj-Cé alkyl may be replaced with -O-;

R⁷ is hydrogen, halogen, or Ci-Cô alkyl wherein said Ci-Cg alkyl is substituted with 0-6 halogen and wherein up to two non-adjacent CH₂ units of said Ci-Cô alkyl may be replaced with -O-; and

X is -PO(OH)₂, -PO(OH)O‘M⁺, -ΡΟ^^Μ*, or -PO(O')₂«D²⁺; M* is a pharmaceutically acceptable monovalent cation; and D²⁺ is a pharmaceutically acceptable divalent cation;

provided that R², R³, R⁵, and R⁷are not simultaneously hydrogen.

2. The compound according to claim 1, wherein R is hydrogen, Cl or CF₃; or R is hydrogen, Cl, CF₃ or CF₂CF₃; or R⁵ is hydrogen, Cl, F, CH3, OCH3 or OCF3; or R⁷ is hydrogen, fluorine or OCF3;orX is-PO(OH)₂.

3. The compound according to claim 1, wherein the compound has formula I-B:

Page 95 oflOO

I-B wherein, independently for each occurrence:

R³ is haiogen, or Ci-Ce alkyl wherein said Ci-Cô alkyl is substituted with 0-6 haiogen;

R⁵ is haiogen, OH, or Cj-Cô alkyl wherein said Ci-Cg alkyl is substituted with 0-6 haiogen and wherein up to two non-adjacent CH₂ units of said Ci-Cô alkyl may be replaced with -O-;

R⁷ is haiogen, or Ci-Cô alkyl wherein said Ci-Cô alkyl is substituted with 0-6 haiogen and wherein up to two non-adjacent CH₂ units of said Ci-Ce alkyl may be replaced with -O-; and

X is -PO(OH)₂, -PO(OH)O‘M⁺, -ΡΟ(Ο')2·2Μ⁺, or -PO(O’)2*D²⁺; M* is a pharmaceutically acceptable monovalent cation; and D²⁺ is a pharmaceutically acceptable divalent cation.

4. The compound according to claim 3, wherein R³ is CF3, Cl or CF2CF3; or R⁵ is F, CH3 or OCH₃; or R⁷ is F; or X is -PO(OH)₂; or X is -PO(OH)O'M⁺, -ΡΟ(Ο')2·2Μ⁺, or -PO(O’)2-D²⁺; M⁺ is Li⁺, Na⁺ or K⁺ and D²⁺ is Mg²⁺ or Ca²⁺.

5. The compound according to claim 3, wherein the compound is (4-(2-(4-fluoro-2- methylphenoxy)-4-(trifluoromethyl)benzamido)-2-oxopyridin-1 (2//)-yl)methyl dihydrogen phosphate; or (4-(2-(4-fluoro-2-methoxyphenoxy)-4-(perfluoroethyl)benzamido)-2-oxopyridinl(227)-yl)methyl dihydrogen phosphate; or (4-(4-chloro-2-(4-fluoro-2-methylphenoxy)benzamido)2-oxopyridin-l(2/7)-yl)methyl dihydrogen phosphate.

6. The compound according to claiml, wherein the compound has formula formula I-A

Page 96 of 100 wherein, independently for each occurrence:

R² is halogen, or Ci-Cô alkyl wherein said Cj-Cô alkyl is substituted with 0-6 halogen;

5 R⁵ is halogen, OH, or Ci-Cô alkyl wherein said Ci-Cô alkyl is substituted with 0-6 halogen and wherein up to two non-adjacent 0¾ units of said Ci-Cg alkyl may be replaced with -O-;

R⁷ is halogen, or Ci-Ce alkyl wherein said Ci-Cg alkyl is substituted with 0-6 halogen and wherein up to two non-adjacent CH₂ units of said Ci-Cô alkyl may be replaced with -O-; and

X is -PO(OH)₂, -PO(OH)O’M⁺, -ΡΟ(Ο’)₂·2Μζ or -PO(O‘)₂«D²⁺; M* is a pharmaceutically

10 acceptable monovalent cation; and D²⁺ is a pharmaceutically acceptable divalent cation; or wherein the compound has formula I-C wherein, independently for each occurrence:

15 R² is halogen, or Ci-Cô alkyl wherein said Ci-Cô alkyl is substituted with 0-6 halogen;

R⁷ is halogen, or Ci-Cô alkyl wherein said Ci-Cô alkyl is substituted with 0-6 halogen and wherein up to two non-adjacent CH₂ units of said Ci-C₆ alkyl may be replaced with -O-; and

X is -PO(OH)₂, —PO(OH)O'M⁺, -PO(O‘)2«2M⁺, or -PO(O')2*D²⁺; M⁺ is a pharmaceutically acceptable monovalent cation; and D²⁺ is a pharmaceutically acceptable divalent cation; or

Page 97 oflOO wherein the compound has formula I-G

I-G wherein, independently for each occurrence:

R² and R³ are independently halogen, or Cj-Cô alkyl wherein said Cj-Cô alkyl is substituted with 0-6 halogen;

R⁵ is halogen, OH, or Ci-Cô alkyl wherein said Ci-Cô alkyl is substituted with 0-6 halogen and wherein up to two non-adjacent CH₂ units of said Ci-Cô alkyl may be replaced with -O-;

R⁷ is halogen, or Ci-Cô alkyl wherein said Ci-Cg alkyl is substituted with 0-6 halogen and wherein up to two non-adjacent CH₂ units of said Ci-C₆ alkyl may be replaced with -O-; and

7. The compound according to claim 6, wherein the compound is (4-(2-(4-fluoro-2- methylphenoxy)-5-(trifhioromethyl)benzamido)-2-oxopyridin-1 (277)-yI)methyl dihydrogen phosphate; or (4-(2-(4-fluorophenoxy)-5-(trifluoromethyl)benzamido)-2-oxopyridin-l(277)-yl)methyl dihydrogen phosphate; or (4-(4,5-dichloro-2-(4-fluoro-2-methoxyphenoxy)benzamido)-2oxopyridin-l(27/)-yl)methyl dihydrogen phosphate.

8. An amorphous Form C of (4-(2-(4-fluoro-2-methylphenoxy)-4-(trifluoromethyl)benzamido)2-oxopyridin-l(277)-yl)methyl dihydrogen phosphate.

Page 98 of 100

9. A crystalline Form B of (4-(2-(4-fhioro-2-methylphenoxy)-4-(trifluoromethyl)benzamido)-2oxopyridin-l(2/7)-yl)methyl dihydrogen phosphate.

10. The crystalline Form B of claim 9, which is characterized by an X-ray powder diffraction pattern (XRPD) comprising at least three approximate peak positions (degrees 2 thêta + 0.2) when measured using Cu Ka radiation, selected from the group consisting of 4.4, 15.2, 16.4, 18.0, 19.1,

19.3, 19.9, 20.2, 20.5, 21.0, 22.2, 23.5 24.2, 24.8, 26.3, 29.6, 30.1 and 31.3, when the XRPD is collected from about 4 to about 40 degrees 2 thêta (2 Θ), or by an X-ray powder diffraction pattern, as measured by Cu Κ« radiation, substantially similar to Figure 2.

11. A process for preparing crystalline Form B of claim 9, comprising contacting (4-(2-(4fluoro-2-methylphenoxy)-4-(trifluoromethyl)benzamido)-2-oxopyridin-1 (277)-yl)methyl dihydrogen phosphate with water, an organic solvent, a mixture of organic solvents or a mixture of an organic solvent and water at a suitable température, stirring for up to 4 weeks and isolating the solid.

12. A pharmaceutical composition comprising the compound according to any one of claims 1-7, the amorphous Form C of claim 8, or the crystalline Form B of claim 9 or 10, and a pharmaceutically acceptable carrier, adjuvant, or vehicle.

13. A compound of any one of claims 1-7, the amorphous Form C of claim 8, the crystalline Form B of claim 9 or 10, or a pharmaceutical composition of claim 12 for use in a method of treating or lessening the severity in a subject of chronic pain, gut pain, neuropathie pain, musculoskeletal pain, acute pain, inflammatory pain, cancer pain, idiopathic pain, multiple sclerosis, Charcot-Marie-Tooth syndrome, incontinence, pathological cough, or cardiac arrhythmia, the method comprising administering an effective amount of the compound, the amorphous Form C, the crystalline Form B, or the pharmaceutical composition to the subject.

14. The compound, the amorphous Form C, the crystalline Form B, or the pharmaceutical composition for use according to claim 13, wherein said subject is treated with one or more additional therapeutic agents administered concurrently with, prior to, or subséquent to treatment with the compound, amorphous Form C, crystalline Form B, or pharmaceutical composition.