OA17143A

OA17143A - Diacylglycerol acyltransferase 2 inhibitors

Info

Publication number: OA17143A
Application number: OA1201400461
Authority: OA
Inventors: Kay AHN; Markus Boehm; Shawn Cabral; Philip A. Carpino; Kentaro Futatsugi; David Hepworth; Daniel W. Kung; Suvi Orr; Jian Wang
Original assignee: Pfizer Inc.
Priority date: 2012-04-06
Filing date: 2013-03-26
Publication date: 2016-03-28

Abstract

Derivatives of purine, 3H-imidazo[4,5-b]pyrimidine and 1H- imidazo[4,5-d]pyrazine of formula I that inhibit the activity of the diacylglycerol acyltransferase 2 (DGAT2) and their uses in the treatment of diseases linked thereto in animals are described herein.

Description

FIELD OFTHE INVENTION

The présent invention relates to new pharmaceutical compounds, pharmaceutical 5 compositions containing these compounds, and their use to inhibit the activity of the diacylglycérol acyltransferase 2 (DGAT2).

BACKGROUND OFTHE INVENTION

Triglycérides or triacylglycerols (TAG) represent a major form of energy storage in mammals. TAG’s are formed by the sequential estérification of glycerol with three îo fatty acids of varying chain lengths and degrees of saturation. TAG synthesized in the intestine or liver are packaged into chylomicrons or very low-density lipoprotein (VLDL), respectively, and exported to peripheral tissues where they are hydrolysed to their constituent fatty acids and glycerol by lipoprotein lipase (LPL). The résultant nonesterified fatty acids (NEFA) can either be metabolised further to produce energy or 15 reesterified and stored.

Under normal physiological conditions, the energy-dense TAG remains sequestered in various adipose depots until there is a demand for its release, whereupon, it is hydrolyzed to glycerol and free fatty acids which are then released into the blood stream. This process is tightly regulated by the opposing actions of insulin 20 and hormones such as catecholamines which promote the déposition and mobilization of TAG stores under various physiological conditions. In the post-prandial setting, insulin acts to inhibit lipolysis, thereby, restraining the release of energy in the form of NEFA and ensuring the appropriate storage of dietary lipids In adipose depots. However, in patients with type 2 diabètes, the ability of insulin to suppress lipolysis is 25 ameliorated and NEFA flux from adipocytes is inappropriately elevated. This, in tum, results in increased delivery of Iipid to tissues such as muscle and liver. In the absence of energetic demand the TAG and other Iipid métabolites, such as diacylglycérol (DAG) can accumulate and cause a loss of insulin sensitivity. Insulin résistance in muscle is characterized by reduced glucose uptake and glycogen storage, whilst in the liver, loss 30 of insulin signaling gives rise to dysreguiated glucose output and over-production of TAG-rich VLDL, a hallmark of type 2 diabètes. Elevated sécrétion of TAG-enriched VLDL, so called VLDL1 particles, is thought to stimulate the production of small, dense low-density lipoprotein (sdLDL), a proatherogenic subfraction of LDL that is associated with elevated risk of coronary heart disease.

Diacylglycérol acyltransferases (DGAT) catalyze the terminal step in TAG synthesis, specifically, the estérification of a fatty acid with diacylglycérol resulting in the 5 formation of TAG. In mammals, two DGAT enzymes (DGAT1 and DGAT2) hâve been characterized. Although these enzymes catalyze the same enzymatic reaction their respective amino acid sequences are unrelated and they occupy distinct gene families. Mice harboring a disruption in the gene encoding DGAT1 are résistant to diet-induced obesity and hâve elevated energy expenditure and activity. Dgatl-/- mice exhibit dysregulated postaborpative release of chylomicrons and accumulate lipid in the enterocytes. The metabolically favorable phenotype observed in these mice is suggested to be driven by loss of DGAT1 expression in the intestine. Importantly, despite a defect in lactation in female Dgatl-/- mice, these animais retain the capacity to synthesize TAG suggesting the existence of additional DGAT enzymes. This observation and the isolation of a second DGAT from the fungus Mortierella rammaniana led to the identification and characterization of DGAT2.

DGAT2 is highly expressed in liver and adipose, and unlike DGAT1, exhibits exquisite substrate specificity for DAG. Délétion of the DGAT2 gene in rodents results in defective intrauterine growth, severe lipemia, impaired skin barrier function, and early 20 post-natal death. Due to the lethality caused by loss of DGAT2, much of our understanding of the physiological rôle of DGAT2 dérivés from studies performed with antisense oligonucleotides (ASO) in rodent models of metabolic disease. In this setting, inhibition of hepatic DGAT2 resulted in improvements In plasma lipoprotein profile (decrease in total cholestérol and TAG) and a réduction of hepatic lipid burden which 25 was accompanied by improved insulin sensitivity and whole-body glucose control. Although the molecular mechanisms underlying these observations are not fully elucidated, it is ciear that suppression of DGAT2 results in a down-regulation of the expression of multiple genes encoding proteins involved in lipogensis, including sterol regulatory element-binding proteins 1c (SREBPIc) and stearoyl CoA-desaturase 1 30 (SCD1 ). In parallel, oxidative pathways are Induced as evidenced by increased expression of genes such as camitine palmitoyl transfersase 1 (CPT1). The net resuit of these changes is to decrease the levels of hepatic DAG and TAG lipid which, in tum, leads to improved insulin responsiveness in the liver. Furthermore, DGAT2 inhibition suppresses hepatîc VLDL TAG sécrétion and réduction ln circulating cholestérol levels.

Fînally, plasma apolipoprotein B (APOB) levels were suppressed, possibly due to decreased supply of TAG for lipidation of the newly synthesized APOB protein. The bénéficiai effects of DGAT2 inhibition on both glycémie control and plasma cholestérol profile support that this target might be valuable in the treatment of metabolic disease.

SUMMARY OF THE INVENTION

The présent application Is directed at compounds of Formula (!)

wherein:

A is CR⁶R⁷, O or S; B is a bond, oxetanyl,

wherein m is 0,1 or 2;

p Is 1,2,3 or 4;

C and D are each Individually selected from N, CH, CF and C(CH₃), wherein only one of C and D is N;

R¹ is -C(0)-heterocyclyl, -C(O)-NR⁴R⁵, or a heteroaryl, wherein said heterocyclyl or heteroaryl Is optionally substituted with 1 or 2 substituents selected independently from (Ci-C4)alky!, (C₃-C_e)cycloalkyl, (CrC₄)alkoxy, (C₃-C6)cycloalkoxy, halo, hydroxy(CrC4)alkyl, mono-N- or di-N,N-(CrC4)alkylamino, mono-N- or di-N,N-(C₃Cejcycloalkylamino, heterocyclyl, hydroxyl and cyano;

R² is (Ct-C4)alkyl, (Ci-C₄)alkoxy, (C₃-Ce)cycloalkyl, (CrCeJcycloalkoxy, aryl, aryloxy, heteroaryloxy, heteroaryl, heterocyclyl, aralkyl, heteroaralkyl, -C(0)-heterocyclyi, C(O)-NR⁴R⁵, or -NR⁴-C(O)-R⁵, wherein alkyl, alkoxy, cycloalkyl, cycloalkoxy, aralkyl, heteroaralkyl, aryl, aryloxy, heteroaryloxy, heteroaryl, heterocyclyl are each optionally substituted with one, two or three substituents selected independently from (CrC₄)alkyl, (C_rC₄)alkoxy, (C₃-Ce)cycloalkyl, (CrCeJcycloalkoxy, -C(O)-(Cr

C₄)alkyl, -C(O)-(C3-C_e)cycloalkyl, halo, -C(O)-(Ci-C₄)alkoxy, -C(0)-(C3Ce)cycloalkoxy, mono-N- or di-N,N-(Ci-C₄)alkylamlno, mono-N- or di-N,N-(C3C_e)cycloalkylamino, (CrC₄)alkylcarbonylamino, (C3-Ce)cycloalkylcarbonylamino, (C_rC₄)alkylcarbonyl-N-(Ci-C₄)alkylamino, (Ci-C₄)alkylcarbonyl-N-(C3Ce)cycloalkylamino, (C₃-Ce)cycloalkylcarbonyl-N-(Ci-C₄)alkylamino, (C3Ce)cycloalkylcarbonyl-N-(C3-Ce)cycloalkylamino, aminocarbonyl, mono-N- or di-N,N(Ci-C₄)alkylaminocarbonyl, mono-N- or di-N,N-(C3-Ce)cycloaminocarbonyl, mono-Nor di-N,N-(Ci-C₄)alkylcarbonyl, mono-N- or di-N,N-(C3-C6)cycloalkylcarbonyl, mono10 N- or di-N,N-(CrC₄)alkoxycarbonyl, mono-N- or di-N,N-(C3-Ce)cycloalkoxycarbonyl, (Ci-C₄)alkylthlo, (C₃-Ce)cycloalkylthio, amlnosulfonyl, (CrC₄)alkylsulfinyl, (CiC₄)alkylsulfonyl, (C3-Ce)cycloalkylsulfinyl, (C3-Ce)cycloalkylsulfonyl, mono-N- or diN,N-(Ci-C₄)alkylamlnosulfonyl, mono-N- or di-N,N-(C3-Ce)cycloalkylaminosulfonyl, (CrC₄)alkylsulfonylamIno, (C3-Ce)cycloalkylsulfonylamino, (CrC₄)alkylsulfonyl-N15 (Ci-C₄)alkylamino, (Ci-C₄)alkylsulfonyl-N-(C3-C6)cycloalkylamino, (C₃Ce)cycloalkylsulfonyl-N-(Ci-C₄)alkylamino, (C3-Ce)cycloalkylsulfonyl-N-(C3Ce)cycloalkylamino, aryl, heteroaryl, heterocyclyl, oxo, carboxyl, amino, hydroxyl and cyano, wherein said alkyl, cycloalkyl, aryl, heteroaryl, heterocyclyl, alkoxy and cydoalkoxy are optionally substituted independently with one to nine fluoro, or 1,2 20 or 3 substituents selected from halo, -C(O)-OH, -C(O)-(Ci-C₄)alkoxy, aminocarbonyl, mono-N- or di-N,N-(CrC₄)alkylcarbonyl, mono-N- or di-N,N-(C3Cejcycloalkylcarbonyl, cyano, amino and hydroxyl;

R³ is (Ci-C₄)alkyl, (C3-Ce)cycloalkyl, hydroxyl or fluoro, wherein said alkyl ls optionally substituted with one to nine fluoros and said (Cs-Cejcycloalkyl ls optionally substituted with one to six fluoros;

R⁴ and R⁵ are each independently selected from hydrogen, (Ci-C4)alkyl, (C3Cejcycloalkyl, aryl, heteroaryl, aryloxy, heteroaryloxy, aralkyl, heteroaralkyl, heterocyclyl, (Ci-C4)alkoxy, and (C3-Ce)cycloalkoxy, wherein R⁴ and R⁵ are each optionally substituted with (Ci-C4)alkyl, (Ci-C₄)alkoxy, (C3-Ce)cycloalkyl, (C330 Ce)cydoalkoxy, halo or cyano, wherein each of said alkyl, cycloalkyl, alkoxy or cydoalkoxy is optionally substituted with one to nine fluoros;

R^e and R⁷ are each independently hydrogen, (Ci-C₄)alkyl, fluoro, (Ci-C₄)alkoxy, hydroxyl or cyano, wherein said alkyl ls optionally substituted with one to nine fluoros;

φ R⁸ is selected from fluoro, methyl or trifluoromethyl;

R⁹ and R¹⁰ are each Independently selected from hydrogen, fluoro, (CrC^alkyl, (C3Ce)cycloalkyl₍ aryl or heteroaryl, wherein said alkyl Is optionally substituted with one to nlne fluoros, and said cycloalkyl Is optionally substituted with one to six fluoros, 5 and said aryl and heteroaryl are optionally substituted with 1,2 or 3 substituents independently selected from fluoro, chloro, methyl, ethyl, Isopropyl, cyclopropyl, methylthlo, methoxy, cyano, trifluoromethyl, difluoromethyl, trifluoromethoxy, difluoromethoxy, oxo and trifluoromethylthio; and n isO, 1 or 2;

or a tautomer thereof or a pharmaceutically acceptable sait of said compound or tautomer.

The présent invention is also directed at pharmaceutical compositions that include a compound of Formula 1 or a tautomer thereof or a pharmaceutically acceptable sait of said compound or tautomer, présent In a therapeutically effective 15 amount, ln admixture with at least one pharmaceutically acceptable excipient.

Furthermore, the présent invention is directed at pharmaceutical compositions that include a compound of Formula 1 or a tautomer thereof or a pharmaceutically acceptable sait of said compound or tautomer, présent in a therapeutically effective amount, in admixture with at least one pharmaceutically acceptable excipient and 20 further including at ieast one additional pharmaceutical agent selected from the group consisting of an anti-obesity agent, an anti-diabetic agent, and a cholesterol/lipid modulating agent.

The présent Invention is also directed at a method for the treatment of diabètes comprising the administration of an effective amount of compound of Formula 1 or a 25 tautomer thereof or a pharmaceutically acceptable sait of said compound or tautomer to a patient in need thereof.

The présent invention is also directed at a method for treating a metabolic or metabolic-related disease, condition or disorder comprising the step of administering to a patient a therapeutically effective amount of a compound of Formula I or a tautomer 30 thereof or a pharmaceutically acceptable sait of said compound or tautomer.

The présent Invention is also directed at a method for treating a metabolic or metabolic-related disease, condition or disorder comprising the step of administering to a patient în need of such treatment two separate pharmaceutical compositions comprising (î) a first pharmaceuticai composition that includes a compound of Formula 1 or a tautomer thereof or a pharmaceutically acceptable sait of said compound or tautomer, présent in a therapeutically effective amount, In admixture with at least one pharmaceutically acceptable excipient.; and (ii) a second composition comprising at least one additional pharmaceuticai agent selected from the group consisting of an anti-obesity agent and an anti-diabetic agent, and at least one pharmaceutically acceptable excipient.

The présent invention is also directed at a method for treating a disease, condition or disorder modulated by the inhibition of DGAT2 in animais comprising the step of administer to an animal in need of such treatment a DGAT2 inhibiting compound or pharmaceutically acceptable sait thereof, wherein the DGAT2 inhibiting compound is a substituted 5-(piperidin-1-yl)-3H-imidazo[4,5-b]pyridine₍ a substituted 5-morpholino3H-imidazo[4,5-b]pyridine, a substituted 6-(piperidin-1-yl)-1H-imidazo[4,5-b]pyrazine, a substituted 6-morpholino-1H-imidazo[4,5-b]pyrazine, a substituted 2-(piperidin-1-yl)-9Hpurine, or a substituted 2-morpholino-9H-purine compound.

It is to be understood that both the foregoing general description and the following detailed description are exemplary and explanatory only and are not restrictive of the invention, as claimed.

BRIEF DESCRIPTION OFTHE DRAWINGS

FIG. 1 is a characteristic x-ray powder diffraction pattern showing a crystalline form of Example 109-B (Vertical Axis: Intensity (CPS); Horizontal Axis: Two thêta (degrees)).

FIG. 2 is a characteristic x-ray powder diffraction pattern showing a crystalline form of Example 109-C (Vertical Axis: Intensity (CPS); Horizontal Axis: Two thêta (degrees)).

FIG. 3 is a characteristic x-ray powder diffraction pattern showing a crystalline form of Example 196-B (Vertical Axis: Intensity (CPS); Horizontal Axis: Two thêta (degrees)).

FIG. 4 is acute effects of DGAT2 inhibitors on plasma TAG levels in Sprague Dawley rats for the Examples 95,108 and 109-A

FIG. 5 represents an observed ¹³C solid state nuclear magnetic résonance spectrum for

Example 109B. The peaks marked by asterisks are spinning sidebands. (Vertical Axis:

Intensity (CPS); Horizonal Axis ¹³C Chemical shift (ppm)).

DETAILED DESCRIPTION OFTHE INVENTION

The présent invention may be understood more readily by reference to the foliowing detailed description of exemplary embodiments of the invention and the examples included therein.

It is to be understood that this invention is not limited to spécifie synthetic methods of making that may of course vary. It is also to be understood that the terminology used herein Is for the purpose of describing particular embodiments only and is not intended to be limiting. ln this spécification and in the claims that follow, reference will be made to a number of terms that shall be defined to hâve the foliowing meanings:

As used herein in the spécification, a* or an may mean one or more. As used herein in the claim(s), when used in conjunction with the word comprising, the words a or an may mean one or more than one. As used herein another may mean at least a second or more.

The term about” refers to a relative term denoting an approximation of plus or minus 10% of the nominal value it refers, in one embodiment, to plus or minus 5%, in another embodiment, to plus or minus 2%. For the field of this disclosure, this level of approximation Is appropriate unless the value is specifically stated require a tighter range.

Compounds” when used herein Includes any pharmaceutically acceptable dérivative or variation, including conformational isomers ie.g., cis and trans isomers) and ail optical isomers (e.g., enantiomers and diastereomers), racemic, diastereomeric and other mixtures of such isomers, as well as solvatés, hydrates, isomorphs, polymorphs, tautomers, esters, sait forms, and prodrugs. By “tautomers is meant chemical compounds that may exist in two or more forms of different structure (isomers) in equilibrium, the forms differing, usually, in the position of a hydrogen atom. Various types of tautomerism can occur, Including keto-enol, ring-chain and ring-ring tautomerism. The expression prodrug” refers to compounds that are drug precursors which foliowing administration, release the drug in vivo via some chemical or physiological process (e.g., a prodrug on being brought to the physiological pH or through enzyme action is converted to the desired drug form). Exemplary prodrugs upon cleavage release the corresponding free acid, and such hydrolyzable ester7 • forming residues of the compounds of the présent invention include but are not limited to those having a carboxyl moiety wherein the free hydrogen is replaced by (CiC^alkyl, (C2-C7)alkanoyloxymethyl, 1-(alkanoyloxy)ethyl having from 4 to 9 carbon atoms, 1-methyl-1-(alkanoyloxy)-ethyl having from 5 to 10 carbon atoms, alkoxycarbonyloxymethyl having from 3 to 6 carbon atoms, 1-(alkoxycarbonyloxy)ethyl having from 4 to 7 carbon atoms, 1-methyl-1-(alkoxycarbonyloxy)ethyl having from 5 to 8 carbon atoms, N-(alkoxycarbonyl)aminomethyl having from 3 to 9 carbon atoms, 1(N-(alkoxycarbonyi)aminoJethyl having from 4 to 10 carbon atoms, 3-phthalidyl, 4crotonolactonyl, gamma-butyrolacton-4-yl, di-N,N-(Ci-C2)alkylamino(C2-C3)alkyl (such as β-dlmethylamlnoethyl), carbamoyl-(CrC2)alkyl, N,N-di(CrC2)alkylcarbamoyl-(CiC2)alkyl and piperidino-, pyrrolidfno- or morpholino(C2-C3)alkyl.

/

As used herein, an arrowhead , ' or wavy line, dénotés a point of attachment of a substituent to another group.

By halo or halogen is meant chloro, bromo, iodo, or fluoro.

By alkyl is meant straight chain saturated hydrocarbon or branched chain saturated hydrocarbon. Exemplary of such alkyl groups (assuming the designated length encompasses the particular example) are methyl, ethyl, propyl, Isopropyl, butyl, sec-butyl, tertiary butyl, isobutyl, pentyl, isopentyl, neopentyl, tertiary pentyl, 1· methylbutyl, 2-methylbutyl, 3-methylbutyl, hexyl, isohexyl, heptyl and octyl.

' By alkoxy” is meant straight chain saturated alkyl or branched chain saturated alkyl bonded through an oxy. Exemplary of such alkoxy groups (assuming the designated length encompasses the particular example) are methoxy, ethoxy, propoxy, isopropoxy, butoxy, isobutoxy, tertiary butoxy, pentoxy, isopentoxy, neopentoxy, tertiary pentoxy, hexoxy, isohexoxy, heptoxy and octoxy.

The term aryl means a carbocyclic aromatic System containing one, two or three rings wherein such rings may be fused. If the rings are fused, one of the rings must be fully unsaturated and the fused ring(s) may be fully saturated, partially unsaturated or fully unsaturated. The term fused means that a second ring is présent (ie, attached or formed) by having two adjacent atoms in common (ie, shared) with the first ring. The term fused Is équivalent to the term condensed. The term aryl embraces aromatic radicals such as phenyl, naphthyl, tetrahydronaphthyl, indanyl, biphenyl, benzo[b][1,4]oxazin3(4H)-onyl, 2,3-dihydro-1 H indenyl, and 1,2,3,4-tetrahydronaphthalenyl.

P The term “aralkyl means an alkyl group with an aryl group (defined above) substituting for a hydrogen atom of the alkyl group. Exemplary of such aralkyl groups are benzyl and phenethyl.

“Aryloxy” means an O-aryl group wherein aryl Is defined above. Exemplary of such aryloxy groups are phenyloxy and naphthyloxy.

“Cycloalkyl refers to a nonaromatic ring that Is fully hydrogenated and exists as a single ring. Examples of such carbocyclic rings include cyclopropyl, cyclobutyl, cyclopentyl, and cyclohexyl.

By “cycloalkoxy” is meant cycloalkyl bonded through an oxy. Exemplary of such cycloalkoxy groups are cyclopropoxy, cyclobutoxy, cyclopentoxy and cyclohexoxy.

The term “heteroaryl means an aromatic carbocyclic System containing one, two, three or four heteroatoms selected Independently from oxygen, nitrogen and sulfur and having one, two or three rings wherein such rings may be fused, wherein fused Is defined above. The term “heteroaryl inciudes but is not limited to furyl, thlenyl, oxazolyl, 15 thiazolyl, imldazolyl, pyrazolyl, triazolyl, tetrazolyl, Isoxazolyl, isothiazolyl, oxadiazoiyl, thiadiazolyl, pyridinyl, pyridiazinyl, pyrimidinyl, pyrazinyl, pyridîn-2(1 H)-onyl, pyridazin2(1H)-onyl, pyrimidin-2(1 H)-onyl, pyrazin-2(1H)-onyl, imidazo[1,2-a]pyridinyl, pyrazolo[1,5-a]pyridinyl, 5,6,7,8-tetrahydrolsoquinolinyl, 5,6,7,8-tetrahydroqulnolinyl,

6.7- dihydro-5H-cyclopenta[b]pyridinyl, 6,7-dihydro-5H-cyclopenta[c]pyridinyl, 1,4,5,620 tetrahydrocyclopenta[c]pyrazolyl, 2,4,5,6-tetrahydrocyclopenta[c]pyrazolyl, 5,6-dihydro-

4H-pyrrolo[1,2-b]pyrazolyl, 6,7-dihydro-5H-pyrrolo[1,2-b][ 1,2,4]triazolyl, 5,6,7,8tetrahydro-[1,2,4]triazolo[1,5-a]pyridinyl, 4,5,6,7-tetrahydropyrazolo[1,5-a]pyridinyl,

4.5.6.7- tetrahydro-1 H-indazolyl and 4,5,6,7-tetrahydro-2H-indazolyl.

The term “heteroaralkyl means an alkyl group with an heteroaryl group substituting for a hydrogen atom of the alkyl group. Exemplary of such heteroaralkyl groups are pyridinyl-(CH2)- and pyrazolyl-fChk)-.

By “heteroaryloxy” Is meant an O-heteroaryl wherein heteroaryl is defined above. Exemplary of such heteroaryloxy groups are pyrazolyloxy, pyridinyloxy and pyrimldlnyloxy.

The term “heterocyclyl means a nonaromatic carbocyclic system containing one, two, three or four heteroatoms selected independently from oxygen, nitrogen and sulfur and having one, two or three rings wherein such rings may be fused, wherein φ fused is defined above. Heterocyclyl also includes bicyclic structures that may be bridged or spirocyclic in nature with each individual ring within the bicycle varying from

3-8 atoms, and containing 0,1, or 2 N, O or S atoms. The term “heterocyclyl” includes but is not limited to lactones, lactams, cyclic ethers and cyclic amines, including the following exemplary ring Systems: pyrrolidinonyl, 2,5-dihydro-1 H-pyrrolyl, piperidinonyl, morpholinonyl, piperazinonyl, oxazolidinonyl, imidazolidinonyl, 1,3-oxazinan-2-onyl, tetrahydropyrimidin-2(1 H)-onyl, epoxidyl, tetrahydrofuranyl, tetrahydropyranyl, dioxanyl, aziridinyl, azetidinyl, oxetanyl, pyrrolldinyl, oxazolidinyi, thiazolidinyl, piperidinyl, morpholinyl, piperazinyl, thiomorpholinyl, 1,3-oxazinanyl, 1,3-thiazinanyl, 210 azabicyclo[2.1.1]hexanyl, 5-azabicyclo[2.1.1]hexanyl, 6-azabicyclo[3.1.1]heptanyl, 2azablcyclo[2.2.1]heptanyl, 3-azablcyclo[3.1.1]heptanyl, 2-azablcyclo[3.1.1]heptanyl, 3azabîcyclo[3.1.0]hexanyl, 2-azabicyclo[3.1.0]hexanyl, 3-azabicyclo[3.2.1]octanyl, 8azabicyclo[3.2.1 joctanyl, 3-oxa-7-azabicyclo[3.3.1 Jnonanyl, 3-oxa-9azabicyclo[3.3.1 ]nonanyi, 2-oxa-5-azabîcyclo[2.2.1 Jheptanyl, 6-oxa-315 azabicyclo[3.1.1 Jheptanyl, 2-azaspiro[3.3]heptanyl and 2-oxa-6-azaspiro[3.3]heptanyl.

It is to be understood that if a carbocyclic or heterocyclic moiety may be bonded or otherwise attached to a designated substrate through differing ring atoms without denoting a spécifie point of attachment, then ail possible points are intended, whether through a carbon atom or, for example, a trivalent nitrogen atom. For example, the term 20 “pyridyl” means 2-, 3- or 4-pyridyl, the term “thienyl” means 2- or 3-thienyl, and so forth.

“Patient refers to warm blooded animais such as, for example, guinea pigs, mice, rats, gerbils, cats, rabbits, dogs, cattle, goats, sheep, horses, monkeys, chimpanzees, and humans.

By “pharmaceutically acceptable” is meant that the substance or composition must be compatible chemically and/or toxicologically, with the other ingrédients comprising a formulation, and/or the mammai being treated therewith.

As used herein, the expressions reaction-inert solvent* and inert solvent* refer to a solvent or a mixture thereof which does not interact with starting materials, reagents, intermediates or products in a manner which adversely affects the yield of the 30 desired product.

As used herein, the term selectivity or sélective* refers to a greater effect of a compound in a first assay, compared to the effect of the same compound in a second 10 • assay. For example, in “gut sélective” compounds, the first assay Is for the half life of the compound in the intestine and the second assay is for the half life of the compound in the liver.

“Therapeutically effective amount” means an amount of a compound of the présent invention that (i) treats or prevents the particular disease, condition, or disorder, (ii) atténuâtes, améliorâtes, or éliminâtes one or more symptoms of the particular disease, condition, or disorder, or (iii) prevents or delays the onset of one or more symptoms of the particular disease, condition, or disorder described herein.

The terni treating, treat or treatment as used herein embraces both preventative, Le., prophylactic, and palliative treatment, Le., relieve, alleviate, or slow the progression of the patient’s disease (or condition) or any tissue damage associated with the disease.

The compounds of the présent invention may contain asymmetric or chiral centers, and, therefore, exist in different stereoisomeric forms. Unless specified is otherwise, it is intended that al! stereoisomeric forms of the compounds of the présent invention as well as mixtures thereof, inciuding racemic mixtures, form part of the présent invention, ln addition, the présent invention embraces ail géométrie and positional isomers. For example, if a compound of the présent invention incorporâtes a double bond or a fused ring, both the c/s- and trans- forms, as well as mixtures, are embraced within the scope of the invention.

Chiral compounds of the invention (and chiral precursors thereof) may be obtained in enantiomerically-enriched form using chromatography, typically high pressure liquid chromatography (HPLC), on a resin with an asymmetric stationary phase and with a mobile phase consisting of a hydrocarbon, typically heptane or hexane, containing from 0 to 50% isopropanol, typically from 2 to 20%, and from 0 to 5% of an alkylamine, typically 0.1% diethylamine (DEA). Concentration ofthe eluent affords the enriched mixture.

Diastereomeric mixtures can be separated into their individual diastereoisomers on the basis of their physical chemical différences by methods well known to those skilled in the art, such as by chromatography and/or fractional crystallîzation. Enantiomers can be separated by converting the enantiomeric mixture into a diastereomeric mixture by reaction with an appropriate optically active compound (e.g.

φ chiral auxiliary such as a chiral alcohol or Mosheris acid chloride), separating the diastereoisomers and converting (e.g. hydrolyzing) the individual diastereoisomers to the corresponding pure enantiomers. Enantiomers can also be separated by use of a chiral HPLC column. Altematively, the spécifie stereoisomers may be synthesized by using an optically active starting material, by asymmetric synthesis using optically active reagents, substrates, catalysts or solvents, or by converting one stereoisomer Into the other by asymmetric transformation.

Where the compounds of the présent invention possess two or more stereogenic centers and the absolute or relative stereochemistry Is given in the name, the désignations R and S refer respectively to each stereogenic center in ascending numerical order (1,2,3, etc.) according to the conventional IUPAC number schemes for each molécule. Where the compounds of the présent invention possess one or more stereogenic centers and no stereochemistry ïs given in the name or structure, it is understood that the name or structure is intended to encompass ail forms of the compound, including the racemic form. The compounds and intermediates described below were named using the naming convention provided with ChemBioDraw Ultra, Version 12.0 (CambridgeSoft Corp., Cambridge, Massachusetts).

The compounds of this invention may contain olefin-like double bonds. When such bonds are présent, the compounds of the invention exist as cis and trans configurations and as mixtures thereof. The term cis refers to the orientation of two substituents with reference to each other and the plane of the ring (either both “up” or both “down). Analogously, the term “trans refers to the orientation of two substituents with reference to each other and the plane of the ring (the substituents being on opposite sides of the ring).

It is also possible that the intermediates and compounds of the présent invention may exist In different tautomeric forms, and ail such forms are embraced within the scope of the invention. The term “tautomef or “tautomeric form refers to structural Isomers of different energies which are interconvertible via a low energy barrier. For example, proton tautomers (also known as prototropic tautomers) include interconversions via migration of a proton, such as keto-enol and Imine-enamine isomerizations. A spécifie example of a proton tautomer is the imidazole moiety where the proton may migrate between the two ring nitrogens. For example, the following îs illustrative of tautomers of the compounds of Formula I.

Valence tautomers include interconversions by reorganization of some of the bonding électrons.

Included within the scope of the claimed compounds present invention are ail stereoisomers, géométrie isomers and tautomeric forms of the compounds of Formula (I), Including compounds exhibiting more than one type of isomerism, and mixtures of one or more thereof. Also included are acid addition or base salts wherein the counterion is optically active, for example, D-lactate or L-lysine, or racemic, for example, 10 DL-tartrate or DL-arginine.

The present invention includes ail pharmaceutically acceptable isotopicallylabelled compounds of Formula (I) wherein one or more atoms are replaced by atoms having the same atomic number, but an atomic mass or mass number different from the atomic mass or mass number usually found in nature.

Examples of isotopes suitable for inclusion in the compounds of the invention include isotopes of hydrogen, such as ²H and ³H, carbon, such as ¹¹C, ¹³C and ¹⁴C, chlorine, such as ³⁶CI, fluorine, such as ¹⁸F, iodine, such as ¹²³l and ¹²⁵l, nitrogen, such as ¹³N and ¹⁵N, oxygen, such as ¹⁵0,¹⁷0 and ¹⁰O, phosphorus, such as “P, and sulphur, such as ³⁵S.

Certain isotoplcally-labelled compounds of Formula (I), for example, those incorporating a radioactive isotope, are useful in drug and/or substrate tissue distribution studies. The radioactive isotopes tritium, i.e. ³H, and carbon-14, i.e. ¹⁴C, are particulariy useful for this purpose in view of their ease of incorporation and ready means of détection.

Substitution with heavier isotopes such as deuterium, i.e. ²H, may afford certain therapeutic advantages resulting from greater metabolic stability, for example, increased in vivo half-life or reduced dosage requirements, and hence may be preferred in some circumstances.

Substitution with positron emitting isotopes, such as ¹¹C, ¹⁸F, ¹⁵O and ¹³N, can be useful in Positron Emission Tomography (PET) studies for examining substrate receptor occupancy.

Isotopically-labelled compounds of Formula (I) can generally be prepared by conventional techniques known to those skilled in the art or by processes analogous to those described ln the accompanying Examples and Préparations using an appropriate isotopically-labelled reagente in place of the non-labelled reagent previously employed.

The compounds of the présent invention may be Isolated and used perse, or when possible, in the form of its pharmaceutically acceptable sait. The term “salts” refers to inorganic and organic salts of a compound of the présent invention. These salts can be prepared ln situ during the final isolation and purification of a compound, or by separately treating the compound with a suitable organic or inorganic acid or base and Isolating the sait thus formed. The acids which are used to préparé the pharmaceutically acceptable acid addition salts of the aforementioned base compounds of this invention are those which form non-toxic acid addition salts, (i.e.. salts containing pharmacologically acceptable anlons, such as the hydrochloride, hydrobromide, hydroïodide, nitrate, sulfate, bisulfate, phosphate, acid phosphate, acetate, lactate, citrate, acid citrate, tartrate, bitartrate, succinate, maleate, fumarate, gluconate, saccharate, benzoate, methanesulfonate, ethanesulfonate, benzenesulfonate, naphthylate, mesylate, glucoheptonate, lactobionate, laurylsulphonate, hexafluorophosphate, benzene sulfonate, tosylate, formate, trifluoroacetate, oxalate, besylate, palmitiate, pamoate, malonate, stéarate, laurate, malate, borate, p-toluenesulfonate and pamoate (i.e., 1,T-methylene-bis-(2-hydroxy-3- naphthoate)) salts.

The invention also relates to base addition salts of the compounds of the présent invention. The chemical bases that may be used as reagents to préparé pharmaceutically acceptable base salts of those compounds of the présent invention that are acidic ln nature are those that form non-toxic base salts with such compounds. Such non-toxic base salts include, but are not limited to those derived from such pharmacologically acceptable cations such as alkali métal cations (e.g., lithium, potassium and sodium) and alkaline earth métal cations (e.g., calcium and magnésium), ammonium or water-soluble amine addition salts such as Nmethylglucamine-(meglumine), tétraméthylammonium, tetraethylammonium, methylamlne, dimethylamine, trimethylamine, triethylamine, ethylamine, and the lower alkanolammonium and other base salts of pharmaceutically acceptable organic amines. See e.g. Berge, et al. J. Pharm. Sci. 66,1-19 (1977).

Certain compounds of the présent Invention may exist in more than one crystal form (generally referred to as “polymorphs). Polymorphs may be prepared by crystallization under various conditions, for example, using different solvents or different solvent mixtures for recrystallization; crystallization at different températures; and/or various modes of cooling, ranging from very fast to very slow cooling during crystallization. Polymorphs may also be obtained by heating or melting the compound of the présent invention followed by graduai or fast cooling. The presence of polymorphs may be determined by solid probe NMR spectroscopy, IR spectroscopy, differential scanning calorimetry, powder X-ray diffraction or such other techniques.

In one embodiment of compounds of Formula 1, R¹ is -C(O)-heterocyclyl, -C(O)NR⁴R⁵, pyridyl, 6,7-dihydro-5H-pyrrolo[2,1-c][1_l2_l4]triazolyl₎ 6,7-dihydro-5H-pyrrolo[1,2cjimidazolyl, wherein R¹ Is optionally substituted with 1 or 2 substituents independently selected from fluoro, methyl, hydroxyl or -CH2OH;

Dis CH, N, or CF;

B is a bond, oxetanyl or

wherein p is 1 or 2;

R³ is fluoro or methyl;

R⁶ and R⁷ are each Independently hydrogen, fluoro or methyl;

R⁸ is selected from fluoro or methyl; and

R⁹ and R¹⁰ are each individually selected from hydrogen, fluoro, or methyl;

In another embodiment of compounds of Formula 1, R¹ is -C(O)-heterocyclyl or C(O)-NR⁴R^S wherein said heterocyclyl is optionally substituted with 1 or 2 substituents independently selected from fluoro and methyl;

B is a bond,

wherein p is1 or 2;

R² is selected from phenyl, furyl, thienyl, oxazolyl, thiazolyl, imidazolyl, pyrazolyl,

1,2,3-triazolyl, 1,2,4-triazolyl, tetrazolyl, isoxazolyl, Isothiazolyl, oxadiazolyl, thiadiazolyl, pyridinyl, pyridîazinyl, pyrimidinyl, pyrazinyl, pyridin-2(1 HJ-onyl, pyridazin-2(1 H)-onyl, pyrimidin-2(1 H)-onyl, pyrazin-2(1 H)-onyl, imidazo[1,2-a]pyridinyl, pyrazolo[1,5ajpyridinyl, 2H-benzo[b][1,4]oxazin-3(4H)-onyl, 2,3-dihydro-1 H-indenyl, 1,2,3,4tetrahydronaphthalenyl, 5,6,7,6-tetrahydroisoquinolinyl, 5,6,7,6-tetrahydroquinolinyl, 6,7dihydro-5H-cyclopenta[b]pyridinyl, 6,7-dihydro-5/7-cyclopenta[c]pyridinyl, 1,4,5,610 tetrahydrocyclopenta[c]pyrazolyl, 2,4₁5₁6-tetrahydrocyclopenta[c]pyrazolyl, 5,6-dihydro4H-pyrrolo[1,2-b]pyrazolyl, 6,7-dihydro-5H-pyrrolo[1,2-b][1,2,4]triazolyl, 5,6,7,6tetrahydro-[1,2,4]triazo!o[1,5-aJpy ridinyl, 4,5,6,7-tetrahydropyrazolofl ,5-a]pyridinyl,

4,5,6,7-tetrahydro-1 H-indazolyl, 4,5,6,7-tetrahydro-2H-indazolyl, phenyloxy, pyridinyloxy, benzyl, pyridinyl-(CH2)-, pyrazolyl-(CH2)-, cyclopropyl, and cyclobutyl; wherein said R² is optionally substituted with 1,2 or 3 substituents independently selected from (C_rC4)alkyl, (Ci-C4)alkoxy, (C₃-C₆)cycloalkoxy, cyclopropyl, halo, hydroxyl, amino, dimethylamino, methylamino, cyclopropylamino, aminocarbonyl, methylaminocarbonyl, (CrC4)alkylthio, (Cs-Cejcycloalkylthio, aminosulfonyl, methylaminosulfonyl, phenyl, and heteroaryl wherein heteroaryl is selected from furyl, thienyl, oxazolyl, thiazolyl, imidazolyl, pyrazolyl, triazolyl, isoxazolyl, isothiazolyl, oxadiazolyl, thiadiazolyl, pyridinyl, pyridîazinyl, pyrimidinyl, pyrazinyl, pyrid1n-2(1 H)-onyl, pyridazin-2(1 H)-onyl, pyrimidin2(1 H)-only, pyrazin-2(1 H)-onyl, oxetanyl, azetidinyl, and pyrrolidinyl, wherein said alkyl, cyclopropyl, azetidinyl, pyrrolidinyl, alkoxy and cydoalkoxy are optionally substituted with oxo, cyano, or up to three fluoro or hydroxy!, and said phenyl or heteroaryl is optionally substituted independently with up to three groups selected from halo, methyl, trifluoromethyl, difluoromethyl, methoxy, trifluoromethoxy, difluoromethoxy, cyano, cyclopropryl, methylthio, oxo and trifluoromethylthio; and

R⁹ and R¹⁰ are each indivîdually selected from hydrogen and methyl;

In a further embodiment of compounds of Formula 1, R¹ is -C(0)-heterocyclyl or C(O)-NR⁴R⁵ wherein said heterocyclyl is selected from pyrrolidinyl, 2,5-dihydro-1 H16 φ pyrrolyl, azetidinyl, piperidinyl and morpholinyl and said heterocyclyl Is optionally substituted with 1 or 2 substituents independently selected from fluoro and methyl;

C is CH, N or CF;

A is CH₂ or O;

R² is phenyl, furyl, thlenyl, oxazolyl, thiazolyl, Imldazolyl, pyrazolyl, triazolyl, tetrazolyl, isoxazolyl, isothiazolyl, oxadiazolyl, thiadiazolyl, pyridtnyl, pyridiazinyl, pyrimldinyl, pyrazinyl, pyridin-2(1H)-onyl, pyridazin-2(1H)-onyl, pyrimidin-2(1 H)-onyl, pyrazin-2(1 H)-onyl, phenyloxy, pyridinyloxy, benzyl, pyridinyl-(CH₂)- or pyrazolyl-(CH₂)-, wherein R² is optionally substituted with 1,2 or 3 substituents independently selected from fluoro, chloro, methyl, ethyl, isopropyl, cyclopropyl, hydroxyl, amino, methylthlo, methoxy, cyano, trifluoromethyl, difluoromethyl, trifluoromethoxy, difluoromethoxy, oxo and trifluoromethylthio;

R⁴ is hydrogen or methyl;

R⁵ is hydrogen or methyl;

is or a tautomer thereof or a pharmaceutically acceptable sait of said compound or tautomer.

ln another embodiment of compounds of Formula I, R¹ is -C(O)-heterocyclyl, wherein said heterocyclyl is selected from pyrrolidinyl, 2,5-dihydro-1 H-pyrrolyl, 3,3difluoroazetidinyl, 3,3-difluoropyrroiidinyl and morpholinyl;

Bis

wherein p is 1 or 2; and

R² is phenyl, furyl, thlenyl, oxazolyl, thiazolyl, Imidazolyl, pyrazolyl, triazolyl, Isoxazolyl, Isothiazolyl, oxadiazolyl, thiadiazolyl, pyridinyl, pyridiazinyl, pyrimidinyl, pyrazinyl, phenyloxy, pyridinyloxy, benzyl, pyridinyl-(CH₂)-, or pyrazolyl-(CH₂)-; wherein R² is optionally substituted with 1,2 or 3 substituents selected from independently fluoro, chloro, methyl, ethyl, isopropyl, cyclopropyl, methylthio, methoxy, cyano, trifluoromethyl, difluoromethyl, trifluoromethoxy, difluoromethoxy, oxo and trifluoromethylthio;

ln another embodiment of compounds of Formula I, R² is N-linked pyrazolyl optionally substituted with 1,2 or 3 substituents independently selected from fluoro, φ chloro, methyl, ethyl, isopropyl, cyclopropyl, hydroxyl, amino, methylthio, methoxy, cyano, trifluoromethyl, difluoromethyi, trifluoromethoxy, difluoromethoxy and trifluoromethylthio or a tautomer thereof or a pharmaceutically acceptable sait of said compound or tautomer.

In another embodiment of compounds of Formula I, R¹ is

Cis CH or CF;

A Is CH₂;

n is 0; and

R² is ΛΖ-linked pyrazolyl substituted at the 4 position with fluoro or chloro;

Another embodiment includes the compounds:

(1-(2-(1 -(4-f luoro-1 H-pyrazol-1 -yl)cyclopropyl)-3H-imidazo[4,5-b]pyridin-5y IJpiperidi n-3-yl) (pyrrolidin-1-y I) methanon e;

(1-(8-(1 -(4-chloro-1 H-pyrazol-1 -yl)cyclopropyl)-9H-purin-2-yl)piperidin-3yl) (pyrrolidin-1 -yljmethanone;

(1-(2-(1 -(4-chloro-1 H-pyrazol-1 -yl)cyclopropyl)-3H-imidazo[4,5-b]pyridin-520 yl)piperidin-3-yl) (pyrrolidin-1 -yljmethanone;

(1 -(2-(2-(4-chloro-1 H-pyrazol-1 -yl)propan-2-yl)-3H-imidazo[4,5-b]pyridin-5yl)piperidin-3-yl)(pyrrolidin-1-yl)methanone;

(1 -{2-[1 -(4-chloro-1 H-pyrazol-1 -yl)cyclopropyl]-3H-imidazo[4,5-b]pyridin-5-yl}(2,2,6,6²H₄)piperidin-3-yl](pyrrolidin-1-yl)methanone;

(1 -(2-((fîJ-1 -(4-fluoro-1 H-pyrazol-1 -yl)ethyl)-3H-imidazo[4,5-b]pyridin-5-yl)piperidin-3yl ) (py rrolidi n-1 -yl)methanone;

(1 -(2-((SJ-1 -(4-fluoro-1 H-pyrazol-1 -ylJethyl)-3H-lmidazo[4,5-b]pyridin-5-yl)piperidin-3yl )(py rrolid i n-1 -yljmethanone;

(1 -(2-(( S)-1 -(4-ch1oro-1 H-pyrazol-1 -yl)ethyl)-3H-imidazo[4,5-b]pyridîn-5-yl)pïperidin30 3-yl)(pyrrolidin-1 -yljmethanone;

P (1 -(2-( (R)-1 -(4-chloro-1 H-pyrazol-1 -yl)ethyl)-3H-imidazo[4,5-b]pyridin-5-yl)piperidin3-yl)(pyrrolidin-1-yl)methanone;

( 1 -(8-((S)-1 -(4-ch!oro-1 H-pyrazol-1 -y I) ethy I) -9 H-purin-2-y I) pipe ridin-3-y I) (pyrrolidin-1 yljmethanone;

(1 -(8-((/7)-1 -(4-ch lo ro-1 H-pyrazol-1 -yljethyl )-9 H- puri n-2-y!) pi peridin-3-y I) ( py rro lidi n-1 yljmethanone; and (1-(8-(1-(4-fluoro-1 H-pyrazol-1 -y!)cyclopropyl)-9 H-purin-2-yl)piperidin-3-yl)(pynrolidin-

1-yljmethanone;

or a tautomer thereof or a pharmaceutically acceptable sait of said compound or îo tautomer.

Another embodiment includes the compounds:

( (R)-1 -(2-((S)-1 -(4-Chloro-1 H-pyrazol-1 -yl)ethyl)-3H-imidazo[4,5-b]pyridin-5yl)piperidin-3-yl)(pyrro!idïn-1-yljmethanone, ((R)-1 -(2-((R)-1 -(4-Chloro-1 H-pyrazol-1 -yl)ethyl)-3H-lmidazo[4,5-b]pyridin-515 yl)piperidin-3-yl) (pyrrolidin-1 -yljmethanone, ( ( S)-1 -(2-((S)-1 -(4-Chloro-1 H-pyrazol-1 -yl)ethyl)-3H-imidazo[4,5-b]pyri dïn-5yl)piperidin-3-yl) (pyrrolidin-1 -yljmethanone, or ( ( S)-1 -(2-((R)-1 -(4-Chloro-1 H-pyrazol-1 -yl)ethyl)-3H-lmidazo[4,5-b]pyridin-5yl)plperidin-3-yl) (pyrrolidin-1-yljmethanone, or a mixture of thereof;

Another embodiment includes the compounds:

(R)-(1 -(2-(1 -(4-chloro-1 H-pyrazol-1 -yl)cyclopropyl)-3H-imidazo[4,5-b]pyridin-525 yl)piperidin-3-yl) (pyrrolidin-1-yljmethanone, and ( S) - ( 1 -(2-(1 -(4-chloro-1 H-pyrazol-1 -yl)cyclopropyl)-3H-imidazo[4,5-b]pyridin-5yl)plperidin-3-yl) (pyrrolidin-1-yljmethanone, or a mixture of thereof;

or tautomer thereof or a pharmaceutically acceptable sait of said compound or tautomer.

Another embodiment includes a compound having the structure:

ci

Another embodiment includes a compound having the structure:

ci

In one embodiment of the pharmaceutical compositions of the present invention, the anti-obesity agent is selected from the group consisting of gut-selective MTP inhibitors (e.g., dirlotapide, mitratapide and Implitapide, R56918 (CAS No. 403987) and CAS No. 913541-47-6), CCKa agonists (e.g., A/-benzyl-2-[4-(1H-indol-3-ylmethyl)-5-oxo-

1-phenyl-4,5-dihydro-2_t3_l6,10b-tetraaza-benzo[e]azulen-6-yl]-A/-isopropyl-acetamide described in PCT Publication No. WO 2005/116034 or US Publication No. 2005-0267100 A1), 5HT2c agonists (e.g., lorcaserin), MCR4 agonist (e.g., compounds described in US 6,818,658), lipase inhibitor (e.g., Cetilistat), PYY3-3e(as used herein “PYY3-36 includes analogs, such as peglated PYY3-36 e.g., those described in US Publication 2006/0178501), opioid antagonists (e.g., naltrexone), the combination of naltrexone with buproprion, oleoyl-estrone (CAS No. 180003-17-2), obinepitide (TM30338), pramlintide (Symlin®), tesofensine (NS2330), ieptin, liraglutide, bromocriptine, oriistat, exenatide (Byetta®), AOD-9604 (CAS No. 221231-10-3) and sibutramîne.

In another embodiment of the pharmaceutical compositions of the present invention, the anti-diabetic agent is selected from the group consisting of an acetyl-CoA carboxylase- (ACC) inhibitor such as those described in WO2009144554, W02003072197, W02009144555 and W02008065508, a diacylglycérol O17143 φ acyltransferase 1 (DGAT-1) inhibitor, such as those described in W009016462 or

WO2010086820, AZD7687 orLCQ908, monoacylglycerol O-acyltransferase inhibitors, a phosphodiesterase (PDE)-10 Inhibitor, an AMPK activator, a sulfonylurea (e.g., acetohexamide, chlorpropamide, diabinese, glibenclamide, glipizide, glyburide, giimepiride, gliclazide, glipentide, gliquidone, glisolamide, tolazamide, and tolbutamïde), a meglitinide, an α-amylase inhibitor (e.g., tendamistat, trestatin and AL-3688), an aglucoside hydrolase inhibitor (e.g., acarbose), an α-glucosldase inhibitor (e.g., adiposine, camiglibose, emiglitate, miglitol, voglibose, pradimicin-Q, and salbostatin), a PPARy agonist (e.g., balaglitazone, ciglitazone, darglitazone, englitazone, isaglitazone, pioglitazone and rosiglitazone), a PPAR α/γ agonist (e.g., CLX-0940, GW-1536, GW1929, GW-2433, KRP-297, L-796449, LR-90, MK-0767 and SB-219994), a biguanide (e.g., metfomnin), a glucagon-like peptide 1 (GLP-1 ) modulator such as an agonist (e.g., exendin-3 and exendin-4), liraglutide, albiglutide, exenatide (Byetta®), albiglutide, lixisenatide, dulaglutide, semaglutide, NN-9924, TTP-054, a protein tyrosine phosphatase-1 B (PTP-1B) inhibitor (e.g., trodusquemine, hyrtiosal extract, and compounds disclosed by Zhang, S., et al., Drug DiscovervTodav. 12(9/10), 373-381 (2007)), SIRT-1 activator (e.g., resveratrol, GSK2245840 or GSK184072), a dipeptidyl peptidease IV (DPP-IV) inhibitor (e.g., those ln W02005116014, sitagliptin, vildagliptin, alogliptin, dutogliptin, linagliptïn and saxagliptin), an insulin secreatagogue, a fatty acid oxidation inhibitor, an A2 antagonist, a c-jun amino-terminal kinase (JNK) inhibitor, glucokinase activa tors (GKa) such as those described in WO2010103437, WO2010103438, WO2010013161, W02007122482, TTP-399, TTP-355, TTP-547, AZD1656, ARRY403, MK-0599, TAK-329, AZD5658 or GKM-001, insulin, an insulin mimetic, a glycogen phosphorylase inhibitor (e.g. GSK1362885), a VPAC2 receptor agonist, SGLT2 inhibitors, such as those described in E.C. Chao et al. Nature Reviews Drug Discovery 9, 551-559 (July 2010) inciuding dapagliflozin, canagliflozin, empagliflozin, tofogliflozin (CSG452), ASP-1941, THR1474, TS-071, ISIS388626 and LX4211 as well as those in WO2010023594, a glucagon receptor modulator such as those described in Demong, D.E. et al. Annual Reports in Médicinal Chemistry 2008,

43,119-137, GPR119 modulators, particulariy agonists, such as those described in

WO2010140092, WO2010128425, WO2010128414, WO2010106457, Jones, R.M. et al. in Médicinal Chemistry 2009,44,149-170 (e.g. MBX-2982, GSK1292263, APD597 and PSN821), FGF21 dérivatives or analogs such as those described ln Kharitonenkov, A. et al. et al., Current Opinion In Investigational Drugs 2009,10(4)359-364, TGR5 (also φ termed GPBAR1 ) receptor modulators, particulariy agonists, such as those described in

Zhong, M., Current Topics in Médicinal Chemistry, 2010,10(4), 386-396 and INT777,

GPR40 agonists, such as those described in Médina, J.C., Annual Reports in Médicinal

Chemistry, 2008,43,75-85, including but not limited to TAK-875, GPR120 modulators, particulariy agonists, high affinity nicotinic acid receptor (HM74A) activators, and SGLT1 inhibitors, such as GSK1614235, listing of anti-diabetic agents found at page 28, line 35 through page 30, line 19 of WO2011005611, inhibitors or modulators of camitine palmitoyl transferase enzymes, inhibitors of fructose 1,6-diphosphatase, inhibitors of aldose reductase, mineralocorticoid receptor Inhibitors, inhibitors of TORC2, inhibitors of

CCR2 and/or CCR5, inhibitors of PKC isoforms (e.g. PKCa, PKCp, PKCy), inhibitors of fatty acid synthetase, inhibitors of serine palmitoyl transferase, modulators of GPR81, GPR39, GPR43, GPR41, GPR105, Kv1.3, retinol binding protein 4, glucocorticoid receptor, somatostain receptors (e.g. SSTR1, SSTR2, SSTR3 and SSTR5), inhibitors or modulators of PDHK2 or PDHK4, inhibitors of MAP4K4, modulators of IL1 family including ILIbeta, modulators of RXRalpha, suitable anti-diabetic agents include mechanisms listed by Carpino, P.A., Goodwin, B. Expert Opin. Ther. Pat, 2010,20(12), 1627-51.

In another embodiment of the pharmaceutical compositions of the présent Invention, the cholesterol/lipid modulating agent is selected from the group consisting of ' 20 HMG-CoA reductase inhibitors (e.g., pravastatin, lovastatin, atorvastatin, simvastatin, fluvastatin, NK-104 (a.k.a. itavastatin, or nisvastatin or nisbastatin) and ZD-4522 (a.k.a. rosuvastatin, or atavastatin or visastatin)); squalene synthetase inhibitors; fibrates; bile acid séquestrants (such as questran); ACAT inhibitors; MTP Inhibitors; lipooxygenase inhibitors; choesterol absorption inhibitors; and cholesteryl ester transfer protein inhibitors. Other atherosclerotic agents include PCSK9 modulators.

In another embodiment, the condition treated is selected from the group consisting of hyperlipidemia, Type I diabètes, Type II diabètes mellitus, idiopathic Type I diabètes (Type lb), latent autoimmune diabètes in adults (LADA), early-onset Type 2 diabètes (EOD), youth-onset atypical diabètes (YOAD), maturity onset diabètes of the 30 young (MODY), malnutrition-related diabètes, gestational diabètes, coronary heart disease, ischémie stroke, restenosis after angioplasty, peripheral vascular disease, intermittent claudication, myocardial Infarction (e.g. necrosis and apoptosis), dyslipldemla, post-prandial lipemia, conditions of impaired glucose tolérance (IGT), conditions of Impaired fasting plasma glucose, metabolic acidosis, ketosis, arthritis, obesity, osteoporosis, hypertension, congestive heart failure, left ventricular hypertrophy, penpheral arterial disease, diabetic retinopathy, macular degeneration, cataract, diabetic nephropathy, glomerulosclerosis, chronlc rénal failure, diabetic neuropathy, metabolic syndrome, syndrome X, premenstrual syndrome, coronary heart disease, angina pectoris, thrombosis, atherosclerosis, myocardial înfarction, transient Ischémie attacks, stroke, vascular restenosis, hyperglycemia, hyperinsulinemia, hyperlipidemia, hypertryglicerïdemia, insulin résistance, impaired glucose metabolism, conditions of impaired glucose tolérance, conditions of impaired fasting plasma glucose, obesity, erectile dysfonction, skin and connective tissue disorders, foot ulcérations and ulcerative colitis, endothélia! dysfonction and impaired vascular compliance, hyper apo B lipoproteinemia, Alzheimeris, schizophrenia, impaired cognition, inflammatory bowel disease, ulcerative colitis, Crohn’s disease, and irritable bowel syndrome, non-alcoholic steatohepatitis (NASH), non-alcoholic fatty liver disease (NAFLD).

In one embodiment, when two compositions are administered, the first composition and the second composition are administered simultaneously. In another embodiment, the first composition and the second composition are administered sequentially and in any order.

Compounds of the présent invention may be synthesized by synthetic routes that include processes analogous to those well-known în the chemical arts, particulariy In light of the description contaïned herein. The starting materials are generally available from commercial sources such as Aldrich Chemlcals (Milwaukee, Wl) or are readily prepared using methods well known to those skilled in the art (e.g., prepared by methods generally described In Louis F. Fieser and Mary Fieser, Reagents for Organic Synthesls, v. 1-19, Wiley, New York (1967-1999 ed.), or Beilsteins Handbuch der organlschen Chemle, 4, Aufl. ed. Springer-Verlag, Berlin, including suppléments (also available via the Beilstein online database)). Many of the compounds used herein, are related to, or are derived from compounds In which there is a large scientific interest and commercial need, and accordingly many such compounds are commercially available or are reported in the literature or are easily prepared from other commonly available substances by methods which are reported in the literature. For example, general methods to synthesize substituted pyrazole dérivatives can be found in Comprehensive Heterocyclic Chemistry II, Elsevier, Oxford, UK, 1996,3,1-75, 817-932; Chem. Rev. 2011, 111,6984-7034; Modem Heterocyclic Chemistry, Wiley-VCH, Weinheim, Germany, 2011, 2, 635-725.

For illustrative purposes, the reaction schemes depicted below provide potential routes for synthesizmg the compounds of the présent invention as well as key intermediates. For a more detailed description of the individual reaction steps, see the Examples section below. Those skilled in the art will appreciate that other synthetic routes may be used to synthesize the inventive compounds. Although spécifie starting materials and reagents are discussed below, other starting materials and reagents can be easily substituted to provide a variety of dérivatives and/or reaction conditions. In addition, many of the compounds prepared by the methods described below can be further modified in light of this disclosure using conventional chemistry well known to those skilled in the art.

In the préparation of the Formula I compounds it Is noted that some of the préparation methods useful for the préparation of the compounds described herein may require protection of remote functionality (e.g., primary amine, secondary amine, carboxyl in Formula I precursors). The need for such protection will vary depending on the nature of the remote functionality and the conditions of the préparation methods. The need for such protection Is readily determined by one skilled in the art. The use of such protection/deprotection methods is also within the skill in the art. For a general description of protecting groups and their use, see T.W. Greene, Protective Groups in Organic Synthesis. John Wiley & Sons, New York, 1991.

For example, certain compounds contain primary amines or carboxylic acid functionalities which may interfère with reactions at other sites of the molécule if left unprotected. Accordingly, such functionalities may be protected by an appropriate protecting group which may be removed In a subséquent step. Suitable protecting groups for amine and carboxylic acid protection include those protecting groups commonly used In peptide synthesis (such as ΛΜ-butoxycarbonyl (Boc), benzyloxycarbonyl (Cbz), and 9-fluorenylmethylenoxycarbonyl (Fmoc) for amines and lower alkyl or benzyl esters for carboxylic acids) which are generally not chemically reactive under the reaction conditions described and can typically be removed without chemically altering other functionality in the Formula I compound.

The Réaction Schemes described below are intended to provide a general description of the methodology employed In the préparation of the compounds of the présent invention. Some of the compounds of the présent invention contain a single chiral center with stereochemical désignation R. In the following Schemes, the general 24 methods for the préparation of the compounds are shown either in racemîc or enantioenriched form. It will be apparent to one skilled in the art that ail of the synthetic transformations can be conducted in a precisely similar manner whether the materials are enantioenriched or racemic. Moreover the resolution to the desired optically active material may take place at any desired point in the sequence using well known methods such as described herein and in the chemistry literature.

In the Reaction Schemes that follow, the variables A, B, C, D, R¹, R², R³, R⁴, R⁵, R⁸, R^e, R¹⁰, m, n and p are as described in the summary except where otherwise noted.

Reaction Scheme I outlines the general procedures that can be used to provide 10 compounds of the présent invention having Formula I.

• Reaction Scheme I

Compounds of the Formula (I) may be synthesized starting from appropriate intermediates through methods described ln the literature such as Synlett 2010, 2759; Tetrahedron Lett. 2009, 50,1780; Tetrahedron Lett. 2006, 47,2883; Synthesis, 2005, 47; J. Org. Chem. 2003, 68, 6814; Tetrahedron Lett. 2002, 43,1893; Tetrahedron Lett. 2001, 42, 751; Synthesis, 2000,1380; Heterocycles 1995,41,1045; J. Org. Chem.

1989, 54,1144; Grimmet, M.R. ln Comprehensîve Heterocyclic Chemistry, 1984, Vol.5, 10 pp 457; Chem. Rev. 1974, 74, 279; J. Heterocycl.Chem. 1970, 7,947; Chem. Rev.

1951, 48, 397. Starting materials (1a) are commercially available or may prepared via methods known to those skilled in the art. The synthèses of some dérivatives (1a) hâve been reviewed (Bioorg. Med. Chem. 2008, 16, 601-635; Tetrahedron 2004, 60,17011729; Tetrahedron 2003, 59, 2953-2989; Synthesis 2004,641-662). The synthèses of 15 starting materials, which may be converted to (1 a) via protecting group or functional group manipulations that are well known to those skilled in the art, are described in the literature. For dérivatives (1a) where R³ is alkyl, methods of synthesis may be utilized such as those described in: J. Med. Chem. 2011, 54,1871-1895; Eur. J. Org. Chem.

2007, 476-486; Org. Lett. 2005, 7, 55-58; Org. Biomol. Chem. 2010, fl, 3635-3637. For 20 dérivatives (1a) where R³ is fluoro, methods of synthesis may be utilized such as those described in: J. Med. Chem. 2010, 53, 7778-7795; J. Fluorine Chem. 2011, 132, 838845; Bioorg. Med. Chem. Lett. 2010, 20, 755-758.

Intermediate (1b) may be prepared from amines (1a) via nucleophilic aromatic substitution of an heteroaryl halide compound in a reaction Inert solvent such as dimethylsulfoxide (DMSO), N,W-dimethylformamide (DMF) or acetonitrile, in the presence of a suitable base, such as triethylamine or diisopropylethylamine at a température between 10°C and 120°C, preferably between 30°C and 110°C. Intermediate (1c) may be prepared from (1b) by a réduction of the nitro group via methods known to those skilled in the art. For example, intermediate (1c) may be prepared from (1b) via hydrogénation in the presence of a suitable hydrogénation catalyst such as palladium on carbon in a réaction inert solvent such as methanol, éthanol or ethyl acetate in the presence or absence of hydrochioric acid at a température between 0°C and 60°C, preferably at ambient température. Altematively, the nitro group can be reduced via dissolving métal réduction with Iron or zinc in the presence of calcium chloride, ammonium chloride or ammonium formate In a suitable solvent such as water, methanol, éthanol or acetic acid at a température between 20°C 15 and 120°C, preferably between 45°C and 100°C.

Compounds of Formula (I) may be prepared from intermediate (1b) and an aldéhyde of the formula R²-BCHO in the presence of sulfur or sodium hydrosulfite and a base such as triethylamine in a suitable solvent such as DMF, éthanol or water at a température between 20°C and 120°C, preferably between 80°C and 110°C.

Altematively, compounds of formula (I) may be prepared in one step from intermediate (1 c) and a carboxylic acid of the formula R²-BCO₂H in the presence of a dehydrating reagent such as triphenylphosphite in organic base such as pyridine or triethylamine at a température between 20°C and 200°C, preferably 200°C. Compounds of Formula (I) may also be prepared ln one step from intermediate (1c) and an imidate of the formula

R²‘BCNH(OR) (wherein R is a small alkyl or fluoroalkyl group such as methyl, ethyl or trifluoroethyl) In the presence of acetic acid, optionally with a base such as triethylamine or diisopropylethylamine, in a suitable solvent such as methanol or éthanol at a température between 20°C and 130°C, preferably between 60°C and 130°C. ln addition, compounds of Formula (I) may be prepared in one step from intermediate (1c) and an aldéhyde of the formula R²-BCHO in the presence of sulfur and a base such as triethylamine in a suitable solvent such as DMF, éthanol or water at a température between 20°C and 120°C, preferably at 110°C.

Compounds of Formula (I) may also be prepared in two steps from intermediate (1 b). Intermediate (1 e) may be prepared from intermediate (1 b) and a carboxylic acid of the formula R²-BCO2H in the presence of an amide coupling reagent, such as propane phosphonic acid anhydride (T₃P) or Ι,Γ-carbonyidÜmîdazole (CDI) in a réaction solvent such as DMF, ethyl acetate, dioxane or toluene in the presence of a base such as triethylamine or diisopropylethylamine In the presence of another organic base such as W,W-dimethyl-4-aminopyridine (DMAP) at a température between 20°C and 150°C, preferably between 40°C and 110°C. Intermediate (1e) may then be converted to compounds of Formula (I) by a réduction of the nitro group and subséquent cyclization reaction via hydrogénation or via dîssolving métal réduction as described above for Intermediate (1 b).

Altematively, compounds of Formula (I) may be prepared from intermediate (1d). Intermediate (1d) may be prepared from intermediate (1c) and a carboxylic acid of the formula R²-BCÜ2H In the presence of an amîde coupling reagent, such as T₃P, CDI, benzotrlazo-1-yloxytris(dimethylamino)phosphonium hexafluorophosphate (BOP), 2(1 H-7-azabenzotriazol-1 -yl)-1,1,3,3-tetramethyl uranium hexafluorophosphate methanaminium (HATU), O-benzotriazol-l-yl-N./V./V’./V-tetramethyluronlum hexafluoro phosphate (HBTU), M-(3-dimethylaminopropyl)-/V-ethylcarbodiimide (EDCI) or 1hydroxybenzotriazole (HOBT) In a réaction Inert solvent such as dichloromethane, DMF or ethyl acetate in the presence of a base such as triethylamine or diisopropylethylamine at a température between 20°C and 140°C, preferably between 40°C and 60°C. Intermediate (1d) may then be converted to compounds of Formula (I) (1 ) In the presence of sodium methoxide or sodium ethoxide In a suitable solvent such as methanol, éthanol, s-butanoi or Isobutanol at a température between 20°C and 120°C, preferably 110°C or (2) In the presence of acetic acid, HCl, polyphospholic acid or para-toluene sulfonic acid in a solvent such as xylene, water, ethylene glycol, 1,4dioxane or acetic acid at a température between 20°C and 200°C.

Reaction Scheme II

Altematively, compounds of Formula (I) may be prepared from intermediate (2b) that can be accessed from intermediate (2a) by incorporating the desired amino protecting 5 group (Pg) as shown in Reaction Scheme II. A preferred amino-protecting group is a carbamate group such as t-butoxycarbonyi (Boc). Intermediate (2a) may be prepared from intermediate (1b) by a réduction of the nitro group via methods known to those skilled in the art or methods described in reaction scheme I, followed by protection of the desired amino group. For example, intermediate (2a) wherein Pg is Boc may be 10 prepared from (1b) via hydrogénation in the presence of a suitable hydrogénation catalyst such as palladium on carbon in the presence of di-tert-butyl dicarbonate ((Boc)₂O) In a reaction inert solvent such as methanol, éthanol or ethyl acetate in the presence or absence of a base such as triethylamine at a température between 0°C and 60°C, preferably between ambient température and 50°C. Altematively, Intermediate 15 (2a) may be prepared from intermediate (1c) by incorporating a suitable amino protecting group by methods known to those skilled In the art. Intermediate (2b) may be prepared from intermediate (2a) and a carboxylic acid of the formula R²-BCO₂H in the presence of an amlde coupling reagent, such as T3P in a reaction solvent such as DMF, ethyl acetate, dioxane or toluene in the presence of a base such as pyridine, 20 triethylamine or dlisopropylethylamine at a température between 0°C and 100°C, preferably between 20°C and 60°C. Intermediate (2b) may then be converted to compounds of Formula (I) by removal of the protecting group and subséquent cyciization reaction under acidic conditions with an acid such as hydrochloric acid, trifluoroacetic acid, acetic acid, methane sulfonic acid or combinations thereof, 25 optionally with a base such as sodium acetate In a solvent such as acetonitrile, ethylacetate, water or combinations thereof at a température between 0°C and 100°C.

φ Reaction Scheme II!

X= Halogen

R²-BCO₂H or br⁷

Alternatively, compounds of Formula (I) may be prepared from intermediate (3a) as shown in Reaction Scheme III. Intermediate (3a) can be prepared from 65 halopyridine-2,3-diamine, 6-halopyrimidine*2,3-diamine or 6-halopyrazine-2,3-diamine and a carboxylic acid of the formula R²-BCO₂H using the conditions described for intermediate (1c) ln Scheme I or with an imidate of the formula R²-B-CNH(OR) (wherein R Is a small alkyl or fluoroalkyl group such as methyl, ethyl or trifluoroethyl) using the conditions described for intermediate (1c) ln Reaction Scheme I. Intermediate (3a) may 10 then be converted to compounds of Formula (I) by nucleophilic aromatic substitution with amines (1a) ln the presence of base such as potassium carbonate and césium fluoride ln a suitable solvent such as dtglyme at a température between 100°C and 150°C, preferably 150°C.

• Reaction Scheme IV

D-C^NOa χ^λν^Χνη₂

X= F, Cl, Brorl

R²-BCHO or

1. hydroîysis

2. R⁴NHR⁵ or heterocyclyl

1. réduction

2. R²-BCO₂H or BR²HN^OR

Compounds of Formula (II) whrein R^a is -NR⁴R⁵ or heterocyclyl optionally substituted with 1 or 2 substituents selected independently from (CrC₄)alkyl, (Cg5 CeJcycloalkyl, (CrC₄)alkoxy, (C3-Ce)cycloalkoxy, halo, hydroxy(Ci-C4)alkyl, mono-N- or di-N,N-(CrC4)alkylamino, mono-N- or di-N,N-(C3-C_e)cycloalkylamino, heterocyclyl, hydroxyl and cyano as described ln the summary above, may be prepared according to Reaction Scheme IV. Intermediate (4a) may be prepared from amino acid esters via nucleophilic aromatic substitution of heteroaryl halide compound as described for intermediate (1a) in Reaction Scheme I. Intermediate (4a) may be converted to intermediate (4b) with an aldéhyde of the formula R²-BCHO by the methods as described In Reaction Scheme I. Altematively, intermediate (4b) may be prepared from intermediate (4a) in two steps via a réduction of the nitro group and reaction of the intermediate diamlne with a carboxylic acid of the formula R²-BCO2H or

Imidate R²-BCNH(OR) (wherein R is a small alkyl or fluoroalkyl group such as methyl, ethyl or trifluoroethyl) as described in Réaction Scheme I. Compounds of Formula (II) (wherein R^a is NR⁴R⁵ or heterocyclyl) may be prepared ln two steps from Intermediate (4b) via hydroîysis of the ester in the presence of an aqueous inorganic base such as sodium hydroxide, lithium hydroxide, potassium hydroxide or potassium carbonate in a 20 suitable solvent such as methanol, éthanol, tetrahydrofuran or water, or a combination thereof at a température between 0°C and 50°C, preferably between 0°C and 20°C, followed by a coupling reaction with amines of formula R⁴NHR⁵or heterocyclyl in the presence of a coupling reagent, such as HATU, HBTU, CDI, HOBT, or EDC1 in the presence of a base such as triethylamine or diisopropylamine and 425 dimethytaminopyridine (DMAP) in a reaction solvent such as dichloromethane, DMF or tetrahydrofuran at a température between 0°C and 50°C, preferably at ambient température, ln addition, compounds of Formula (II) may be prepared according to procedures shown in Reaction Scheme I.

Reaction Scheme V

R¹.

R*NHR^S pCI₃ or c μ Db

Ü - < ° 'ν-Λ¹ ⁰ <*> _(lll)

Compounds of Formula (III) whrein R^b is -NR⁴R⁵ or heterocyclyl, may be prepared using intermediate (5a) as shown ln Reaction Scheme V. Intermediate (5a) may be prepared from intermediate (1c) and methyl 2,2,2-trichloroacetimidate in the presence of 10 formic acid in a suitable solvent such as 2,2,2-trifiuoroethanol at a température between 20°C and 100°C, preferably between 20°C and 60°C. Intermediate (5a) may be converted to compounds of Formula (III) (wherein R^b is NR⁴R⁵ or heterocyclyl) with an amine of formula R⁴NHR⁵ or a heterocyclyl in a suitable solvent, such as 2,2,2trifiuoroethanol at a température between 20°C and 100°C, preferably at 60°C.

Reaction Scheme VI

(SM3) (SM3) _{x=LeavtngGroup}

Starting materials of formula SM1, SM1', SM2, SM2’, SM3 and SM3’ may be prepared according to Reaction Scheme VI. Those skilled in the art will recognize that s there existe a variety of methods for preparing Intermediate (6a). For example, intermediate (6a) may be prepared from the formula X-CH2-CN (X Is leaving group) such as bromoacetonitrile and a nucleophile to întroduce the R² moiety. The nucleophilic reactant may be defined as R²H, where R² is defined as for the compound of formula I, and the H-atom Is located on a heteroatom (N,O or S). Examples of 10 R²H reactants would include but are not limited to heteroaryl or heterocycle containing NH groups (such as pyrazoles, pyridones, morpholinones etc), or phenolic compounds, in the presence of a base such as sodium hydride, potassium carbonate, sodium carbonate or césium carbonate in a solvent such as acetonitrile, ethyl acetate, tetrahydrofuran, DMF or a combination of thereof at a température between 0°C and 15 100°C. Intermediate (6b) may be prepared from acetonitriles (6a) and an alkane containing two leaving groups, such as dibromoethane (when m is 0 and p is 1) in the presence of a base such as sodium hydroxide and fert-butylammonium bromide in a water or sodium hydride in a solvent such as toluene, DMF or DMSO at a température between 0°C and 25°C. Intermediate (6b', R¹⁰ is H) may be prepared analogously from 20 acetonitriles (6a) and an alkylating agent of the formula R⁹-X such as iodomethane

V using the methods described for intermediate (6b). Under the analogous reaction conditions, subséquent reaction with the second alkylating agent of the formula R¹⁰-X may be performed for the synthesis of intermediate (6b*, R¹⁰ is not H). In addition,

Intermediates (6b) and (6b*) may be prepared from intermediate (6h) and (6h*) respectively, and R²-X by (1 ) nucleophilic displacement reaction using the methods described In the literature such as J. Org. Chem. 2005, 70,10186-10169 and J. Am. Chem. Soc. 2000, 122,712-713 or (2) a transition métal mediated arylation reaction (when R² Is aryl or heteroaryl) using the methods described in the literature such as J. Am. Chem. Soc. 2002, 124, 9330-9371, J. Org. Chem. 2003, 68,8003-8007 or Angew.

Chem. Int. Ed. 2003, 42, 5031-5053. Intermediates (6c) and (6c’) may be prepared from intermediates (6i) and (6i*) and R²-X in a similar manner to that described for Intermediates (6b) and (6b*) via a nucleophilc displacement reaction, or a transition métal mediated arylation reaction (when R² Is aryl or heteroaryl) using the methods reported in the literature such as Org. Lett. 2008, 10,1545-1548, Org. Lett. 2008, 10,

1549-1552 or J. Am. Chem. Soc. 2002, 124,12557-12565. Intermediates (6b) and (6b*) may be converted to SM1 and SM1* respectively in the presence of an aqueous Inorganic base such as sodium hydroxide, lithium hydroxide, or potassium hydroxide in a suitable solvent such as methanol, éthanol, tetrahydrofuran or water at a température between 0°C and 50’C, preferably between 0°C and 20°C. Intermediates (6b) and (6b*) may be converted to SM2 and SM2’ respectively in the presence of hydrochloric acid or acetyl chloride in a solvent such as éthanol or methanol at a température between 0°C and 70°C. Altematively, intermediates (6b) and (6b*) may be converted to SM2 and SM2’ respectively in the presence of sodium ethoxide or sodium methoxide in a solvent such as éthanol or methanol at a température between 0°C and 80°C. Intermediate (6c) may be prepared from acetate dérivatives (6d) and an alkane containing two leaving groups, such as dibromoethane (when m Is 0 and p is 1) in the presence of a base, such as césium carbonate, potassium carbonate, sodium carbonate or sodium hydride in a reaction solvent such DMF or DMSO at a température between 0°C and 25°C. Intermediate (6b‘, R¹⁰ is H) may be prepared analogously from acetate dérivatives (6d) and an alkylating agent of formula R⁹-X such as iodomethane using the methods described for Intermediate (6c). Subséquent reaction with a second alkylating agent of formula R¹⁰-X may be performed for the synthesis of Intermediate (6c*. R¹⁰ is not H). If R is an alkyl group, intermediates (6c) and (6c’) may be converted to SM1 and SM1’ respectively (1) in the presence of an aqueous Inorganic base such as sodium hydroxide, lithium hydroxide, potassium hydroxide or potassium carbonate in a suitable solvent such as methanol, éthanol, tetrahydrofuran or water at a température between 0°C and 50°C, preferably between 0°C and 20°C, or (2) in the presence of an an aqueous acid such as hydrochloric acid in a suitable solvent such as water at a s température between 0°C and 120°C. If R¹ îs a benzyl group, intermediates (6c) and (6c') may be converted to SM1 and SM1' respectively via hydrogénation in the presence of palladium on carbon in a reaction solvent such as methanol, éthanol or ethyl acetate at ambient température. If R is a fert-butyl group, intermediates (6c) and (6c’) may be converted to SM1 and SM1’ respectively in the presence of an acid, such as trifluoroacetic acid, hydrochloric acid, methanesulfonic acid in a solvent such as dichloromethane, dioxane, toluene, methanol or ethyl ether at a température between 0°C and 45°C. Altematively, intermediate (6c) may be prepared from (6e) and a nucleophilic reactant R²H, where R² is defined as for the compound of formula I, and the H-atom is located on a heteroatom (N,O or S). Examples of R²H reactants would is include but not limited to heteroaryl or heterocycle containing NH groups (such as pyrazoles, pyridones, morpholinones etc), or phenolic compounds, in the presence of a base such as sodium hydride or potassium trimethylsilanolate in a reaction solvent such as tetrahydrofuran, 2-methyl tetrahydrofuran, DMF, DMSO or combinations thereof at a température between 0°C and 40°C. In addition, intermediates (6c) and (6c’) may be prepared from the suitable alkylating agent (6f) for intermediate (6c), (6f) for intermediate (6c’)) and a nucleophilic reactant R²H, where R² is defined as for the compound of formula I, and the H-atom is located on a heteroatom (N,O or S). Examples of R²H reactants would include but not limited to heteroaryl or heterocycle containing NH groups (such as pyrazoles, pyridones, morpholinones etc), or phenolic compounds, n the presence of a base such as sodium hydride, potassium carbonate, sodium carbonate or césium carbonate in a solvent such as acetonitrile, ethyl acetate, tetrahydrofuran or DMF or a combination of thereof at a température between 0°C and 100°C. Intermediates (6b) and (6b') may be prepared from (6g) and (6g’) respectively, and a nucleophilic reactant R²H, where R² is defined as for the compound of formula I, and the H-atom is located on a heteroatom (N,O or S). Examples of

R²H reactants would include but not limited to heteroaryl or heterocycle containing NH groups (such as pyrazoles, pyridones, morpholinones etc), or phenolic compounds, in the manner described for intermediates (6c) and (6c’). Starting materials of formula SM3 and SM3’ may be prepared through the réduction of SM1, SM1’, SM2, SM2’, (6c) or (6c’) using the methods well-known to those skilled in the art. ln addition, starting matenals of formula SM1 and SM1' may be prepared from SM3 and SM3' respectively by methods known to those skilled in the art.

Reaction Scheme VII

Compounds of Formula (IV) and (V) may be prepared according to Reaction Scheme VII. Compounds of Formula (IV) may be prepared from compounds of formula (i) wherein D Is CH, by reaction with an electrophilic fluorinating agent such as Seiectfluort© ln a solvent such as acetonitrile or DMF at a température between 0°C and 10O’C. Altematively, compounds of Formula (IV) may be prepared from the Intermediate (7a) via (1) fluorination of the corresponding organometallic species prepared by halogen-metal exchange reaction of NH-protected Intermediate (7a), anaiogous to the methods described in W02008080015, or (2) nucleophilic fluorination with a fluorinating agent such as potassium fluoride in the presence or absence of a crown ether such as 18-crown-6 ln a polar solvent such as DMSO or NMP at a température between 20°C and 180°C. Intermediate (7a) may be prepared from compounds of Formula (I) wherein D Is CH, by reaction with an electrophilic halogenating agent such as /V-Bromosuccinimide (NBS) or Mlodosuccinimlde (NIS) ln a solvent such as dîchloromethane, DMF or acetonitrile at a température between 0°C and 100°C. Compounds of the formula (V) may be prepared from the intermediate (7a) via a transition métal mediated coupling reaction with the appropriate métal species such as methyl boronlc acid by the methods described by Hikawa et al in Tetrahedron, 2010, 66,9552-9559 or other methods known to those skilled In the art.

φ Reaction Scheme VIII ^rÊ\Na _L n-po FG-B (8b) FG- CHO, COjH orCNHOR

PG» Protecting group E«CH₂,0, nr·, S q=0,1.2 X· Leaving group Compounds of Formula (VI) may be prepared through the intermediate (8a), that can be accessed from intermediate (1b) and intermediate (8b) (wherein E Is CH₂, O,

N R® or S, q is 0,1 or 2, and R^c and R^e are groups described as optional substituents for R² in the summary above) by the methods described in Reaction Scheme l-IV, or Vil, as shown In Reaction Scheme VIII. Compounds of Formula (VI) may be prepared by removal of the amino-protecting group such as Boc using the methods described In

T.W. Greene, Protective Groups In Organic Synthesis, John Wiley & Sons, New York, 10 1991, followed by reaction with the appropriate electrophilic agent R^d-X (wherein R^d Is a group described as an optional substituent for R² in the summary above, X is leaving group) such as heteroaryl halide, acid chloride, chloroformate or sulfonyl chloride under conditions well known to those skilled in the art.

Reaction Scheme IX

E=O

X= Leaving group

(I): B= bond

m=1 or 2

Compounds of Formula (1) wherein B is a bond may be prepared according to

Reaction Scheme IX. An appropriate nitrogen-protecting group such as [2(Trimethylsilyl)ethoxy]methyl (SEM) group may be introduced for Intermediate (1c) or (9a-e) depending on the nature of remote functionality and the conditions of the préparation methods. The methods for protection/deprotection of such nitrogenprotecting groups can be found in T.W. Greene, Protective Groups in Organic Synthesis, John Wiley & Sons, New York, 1991. Compounds of Formula (I) wherein B is a bond and R² is aryloxy, ΛΖ-linked heterocyclyl or AAlinked heteroaryl, may be prepared from the intermediate (9b) or (9d) and R²-H with a base such as sodium hydride, césium carbonate, potassium carbonate or sodium carbonate in a solvent such as acetonitrile, DMF or DMSO at a température between 0°C and 100°C, or by the methods described in W02007039297 or J. Med. Chem. 2006, 49, 3719-3742, or other methods known to those skilled in the art. Compounds of Formula (I) wherein B is a bond and R² Is Clinked, may be prepared via a transition métal mediated coupling reaction starting from the intermediate (9b) and R²-metal species such as boronic acid dérivatives by the methods described by Grivas et al in J. Heterocyclic. Chem., 1995, 32,467-471 or other methods known to those skilled in the art. Intermediate (9b) and (9d) may be prepared from Intermediate (1c) using the methods described in W02007039297 or J. Med. Chem. 2006, 49, 3719-3742, or other methods known to those skilled in the art. ln addition, intermediate (9b) wherein D is N may be prepared from the Intermediate (9e) (accessible from the intermediate (1c) as described in reaction scheme I, Il or IV) using the methods described in J. Med. Chem. 2011, 54, 655-668.

COMBINATION AGENTS

The compounds of the présent invention can be administered alone or in combination with one or more additional therapeutic agents. By administered in combination or combination therapy* it is meant that a compound of the présent invention and one or more additional therapeutic agents are administered concurrently to the mammal being treated. When administered in combination each component may be administered at the same time or sequentially in any order at different points in time. Thus, each component may be administered separately but sufficiently dosely in time so as to provide the desired therapeutic effect. Thus, the methods of prévention and treatment described herein include use of combination agents.

The combination agents are administered to a mammal in a therapeutically effective amount. By therapeutically effective amount it is meant an amount of a compound of the présent Invention that, when administered alone or in combination with an additional therapeutic agent to a mammal, is effective to treat the desired disease/condition e.g., obesity, diabètes, and cardiovascular conditions such as antihypertensive agents and coronary heart disease.

Examples of suitable anti-diabetic agents include (e.g. insulins, metfomin, DPPIV inhibitors, GLP-1 agonists, analogues and mimetics, SGLT1 and SGLT2 inhibitors). Suitable anti-diabetic agents include an acetyl-CoA carboxylase- (ACC) inhibitor such as those described in WO2009144554, W02003072197, WO2009144555 and W02008065508, a diacylglycérol O-acyltransferase 1 (DGAT-1) inhibitor, such as those described in W009016462 or WO2010086820, AZD7687 or LCQ908, monoacylglycerol O-acyltransferase inhibitors, a phosphodiesterase (PDE)-10 inhibitor, an AMPK activator, a sulfonylurea (e.g., acetohexamide, chlorpropamide, diabinese, glibenciamide, giipizide, glyburide, glimepiride, gliclazide, glipentide, gliquidone, glisolamide, tolazamide, and tolbutamide), a meglitinide, an α-amylase Inhibitor (e.g., tendamistat, trestatin and AL-3688), an α-glucoside hydrolase inhibitor (e.g., acarbose), an α-glucosidase inhibitor (e.g., adiposine, camiglibose, emiglitate, miglitol, voglibose, pradimicin-Q, and salbostatin), a PPARy agonist (e.g., balaglitazone, ciglitazone, darglitazone, englitazone, isaglitazone, pioglitazone and rosiglitazone), a PPAR α/γ agonist (e.g., CLX-0940, GW-1536, GW-1929, GW-2433, KRP-297, L-796449, LR-90, MK-0767 and SB-219994), a biguanide (e.g., metformin), a glucagon-like peptide 1 (GLP-1) modulator such as an agonist (e.g., exendin-3 and exendin-4), liraglutide, albiglutide, exenatide (Byetta®), aibiglutide, lixisenatide, duiaglutide, semaglutide, NN9924, TTP-054, a protein tyrosine phosphatase-1B (PTP-1B) inhibitor (e.g., trodusquemine, hyrtiosal extract, and compounds disclosed by Zhang, S., et al., Drug Discoverv Todav, 12(9/10), 373-381 (2007)), SIRT-1 activator (e.g., resveratrol, GSK2245840 or GSK184072), a dipeptidyl peptidease IV (DPP-IV) inhibitor (e.g., those In W02005116014, sitagliptin, vildagliptin, alogliptin, dutogliptin, linagliptin and saxagliptin), an insulin secreatagogue, a fatty acid oxidation inhibitor, an A2 antagonist, a c-jun amino-terminal kinase (JNK) inhibitor, glucokinase activators (GKa) such as those described In WO2010103437, WO2010103438, W02010013161, W02007122482, TTP-399, TTP-355, TTP-547, AZD1656, ARRY403, MK-0599, TAK329, AZD5658 or GKM-001, insulin, an insulin mimetic, a glycogen phosphorylase • inhibitor (e.g. GSK1362885), a VPAC2 receptor agonist, SGLT2 inhibitors, such as those described in E.C. Chao et al. Nature Reviews Drug Discovery 9, 551-559 (July 2010) including dapaglifiozin, canagliflozin, empagliflozin, tofogliflozin (CSG452), ASP1941, THR1474, TS-071, ISIS388626 and LX4211 as well as those in WO2010023594, 5 a glucagon receptor modulator such as those described in Demong, D.E. et al. Annual Reports in Médicinal Chemistry 2008, 43,119-137, GPR119 modulators, particulariy agonists, such as those described in WO2010140092, WO2010128425, W02010128414, WO2010106457, Jones, R.M. et al. in Médicinal Chemistry 2009,44, 149-170 (e.g. ΜΒΧ-29Θ2, GSK1292263, APD597and PSN821), FGF21 dérivatives or 10 analogs such as those described in Kharitonenkov, A. et al. et al., Current Opinion in Investigational Drugs 2009,10(4)359-364, TGR5 (also termed GPBAR1) receptor modulators, particulariy agonists, such as those described in Zhong, M., Current Topics In Médicinal Chemistry, 2010,10(4), 386-396 and INT777, GPR40 agonists, such as those described in Médina, J.C., Annual Reports in Médicinal Chemistry, 2008, 43, 7515 85, including but not limited to TAK-875, GPR120 modulators, particulariy agonists, high affinity nicotinic acid receptor (HM74A) activators, and SGLT1 inhibitors, such as GSK1614235. A further représentative listing of anti-diabetic agents that can be combined with the compounds of the présent invention can be found, for example, at page 28, line 35 through page 30, line 19 of WO2011005611. Preferred anti-diabetic 20 agents are metformin and DPP-IV inhibitors (e.g., sitagliptin, vildagliptin, alogliptin, dutogliptin, linagliptin and saxagliptin). Other antidiabetic agents could include inhibitors or modulators of camitine palmitoyl transferase enzymes, inhibitors of fructose 1,6diphosphatase, inhibitors of aldose reductase, mineralocorticoid receptor inhibitors, inhibitors of TORC2, inhibitors of CCR2 and/or CCR5, inhibitors of PKC isoforms (e.g.

PKCa, ΡΚΟβ, PKCy), inhibitors of fatty acid synthetase, inhibitors of serine palmitoyl transferase, modulators of GPR81, GPR39, GPR43, GPR41, GPR105, Kv1.3, retinol binding protein 4, glucocorticoid receptor, somatostain receptors (e.g. SSTR1, SSTR2, SSTR3 and SSTR5), inhibitors or modulators of PDHK2 or PDHK4, inhibitors of MAP4K4, modulators of IL1 family including ILIbeta, modulators of RXRalpha. In addition suitable anti-diabetic agents include mechanisms listed by Carpino, P.A., Goodwin, B. Expert Opin. Ther. Pat, 2010,20(12), 1627-51.

Suitable anti-obesity agents include 11β-hydroxy steroid dehydrogenase-1 (11βHSD type 1 ) inhibitors, stearoyl-CoA desaturase-1 (SCD-1) inhibitor, MCR-4 agonists, cholecystokinin-A (CCK-A) agonists, monoamine reuptake inhibitors (such as sibutramine), sympathomimetic agents, Ba adrenergic agonists, dopamine agonists (such as bromocriptine), melanocyte-stimulating hormone analogs, 5HT2c agonists, melanin concentrating hormone antagoniste, leptin (the OB protein), leptin analogs, leptin agonists, galanln antagoniste, lipaee inhibitors (euch ae tetrahydrolipetatin, I.e. orlietat), anorectic agente (euch ae a bombeein agoniet), neuropeptide-Y antagoniete (e.g., NPY Y5 antagoniete), PYY3.36 (including analoge thereof), thyromimetic agente, dehydroepiandroeterone or an analog thereof, glucocorticoid agoniete or antagoniete, orexin antagoniete, giucagon*like peptide-1 agoniete, ciliary neurotrophic factors (such as Axokine™ available from Regeneron Pharmaceuticals, Inc., Tarrytown, NY and Procter & Gamble Company, Cincinnati, OH), human agouti-related protein (AGRP) inhibitors, ghrelin antagoniste, histamine 3 antagoniste or inverse agonists, neuromedin U agonists, MTP/ApoB inhibitors (e.g., gut-selective MTP inhibitors, such as dirlotapide), opioid antagonist, orexin antagonist, the combination of naltrexone with buproprion and the like.

Preferred anti-obesity agents for use in the combination aspects of the présent invention include gut-selective MTP inhibitors (e.g., dirlotapide, mitratapide and implitapide, R56918 (CAS No. 403987) and CAS No. 913541-47-6), CCKa agonists (e.g., /V-benzyl-2-[4-(1 H-indol-3-yimethyl)-5-oxo-1 -phenyl-4,5-dihydro-2,3,6,1 Ob-tetraazabenzo[e]azulen-6-yll-M-lsopropyl-acetamide described in PCT Publication No. WO 2005/116034 or US Publication No. 2005-0267100 Al ), 5HT2c agonists (e.g., lorcaserin), MCR4 agonist (e.g., compounds described in US 6,818,658), lipase Inhibitor (e.g., Cetilistat), PYY3oe(as used herein ΡΥΥ&οβ inciudes analogs, such as peglated PYY306 θ·9·. those described in US Publication 2006/0178501), opioid antagonists (e.g., naltrexone), the combination of naltrexone with buproprion, oleoyl-estrone (CAS No. 180003-17-2), obinepitide (TM30338), pramlintide (Symiin®), tesofensine (NS2330), leptin, liraglutide, bromocriptine, orlistat, exenatide (Byetta®), AOD-9604 (CAS No. 221231-10-3) and sibutramine. Preferably, compounds of the présent invention and combination thérapies are administered In conjunction with exercise and a sensible dîet.

The compounds of the présent invention may be used in combination with cholestérol modulating agents (including cholestérol lowering agents) such as a lipase inhibitor, an HMG-CoA reductase inhibitor, an HMG-CoA synthase inhibitor, an HMGCoA reductase gene expression inhibitor, an HMG-CoA synthase gene expression inhibitor, an MTP/Apo B sécrétion inhibitor, a CETP inhibitor, a bile acid absorption inhibitor, a cholestérol absorption inhibitor, a cholestérol synthesis inhibitor, a squalene 41 φ synthetase inhibitor, a squalene epoxidase inhibitor, a squalene cyclase inhibitor, a combined squalene epoxidase/squalene cyclase inhibitor, a fibrate, niacin, an ionexchange resin, an antioxidant, an ACAT inhibitor or a bile acid séquestrant or an agent such as mipomersen.

Examples of suitable cholesterol/lipid lowering agents and lipîd profile thérapies include: HMG-CoA reductase inhibitors (e.g., pravastatin, lovastatin, atorvastatin, simvastatin, fluvastatin, N K-104 (a.k.a. itavastatin, or nisvastatin or nisbastatin) and ZD-4522 (a.k.a. rosuvastatin, or atavastatin or visastatin)); squalene synthetase inhibitors; fibrates; bile acid séquestrants (such as questran); ACAT inhibitors; MTP io inhibitors; lipooxygenase inhibitors; choesterol absorption inhibitors; and cholesteryl ester transfer protein inhibitors. Other atherosclerotic agents include PCSK9 modulators.

In another embodiment, a compound of Formula I may be co-administered with agents for the treatment of non-alcoholic steatohepatitis (NASH) and/or non-alcoholic 15 fatty liver disease (NAFLD), such as Oriistat, TZDs and other insulin sensitizing agents, FGF21 analogs, Metformin, Omega-3-acid ethyl esters (e.g. Lovaza), Fibrates, HMG CoA-reductase Inhibitors, Ezitimbe, Probucol, Ursodeoxycholic acid, TGR5 agonîsts, FXR agonîsts, Vitamin E, Betaine, Pentoxifylline, CB1 antagoniste, Camitine, Nacetylcysteine, Reduced glutathione, lorcaserin, the combination of naltrexone with 20 buproprion, SGLT2 Inhibitors, Phentermine, Topiramate, Incretin (GLP and GIP) analogs and Angiotensin-receptor blockers.

Additional therapeutic agents include anti-coagulant or coagulation inhibitory agents, anti-platelet or platelet inhibitory agents, thrombin inhibitors, thrombolytic or fibrinolytic agents, anti-arrythmlc agents, anti-hypertensive agents, calcium channel 25 blockers (L-type and T-type), cardiac glycosides, diruetics, mineralocorticoid receptor antagonists, NO donating agents such as organonitrates, NO promotlng agents such as phosphodiesterase inhibitors, cholesterol/lipid lowering agents and lipid profile thérapies, anti-dlabetic agents, anti-depressants, anti-inflammatory agents (steroidal and non-steroidal), anti-osteoporosis agents, hormone replacement thérapies, oral 30 contraceptives, anti-obesity agents, anti-anxiety agents, anti-proliferative agents, antitumor agents, anti-ulcer and gastroesophageal reflux disease agents, growth hormone and/or growth hormone secretagogues, thyroid mimetics (induding thyroid hormone receptor antagonist), anti-infective agents, anti-viral agents, anti-bacterial agents, and anti-fungal agents.

Agents used in an ICU setting are included, for example, dobutamine, dopamine, dpinephrine, nitroglycerin, nitroprusside etc.

Combination agents useful for treating vasculitis are included, for example, azathioprine, cyclophosphamide, mycophenolate, mofetil, rituxlmab etc.

In another embodiment, the présent invention provides a combination wherein the second agent is at least one agent selected from a factor Xa inhibitor, an anticoagulant agent, an anti-platelet agent, a thrombin inhibiting agent, a thrombolytîc agent, and a fibrinolytic agent. Exemplary factor Xa inhibitors include apixaban and rivaroxaban. Examples of suitable anti-coagulants for use ln combination with the compounds of the présent invention include heparins (e.g., unfractioned and low molecular weight heparins such as enoxaparin and dalteparin).

ln another preferred embodiment the second agent is at least one agent selected from warfarin, dabigatran, unfractionated heparin, low molecular weight heparin, synthetic pentasaccharide, hirudin, argatrobanas, aspirin, ibuprofen, naproxen, sulindac, indomethacin, mefenamate, droxicam, diclofenac, sulfinpyrazone, piroxicam, tîclopidine, clopidogrel, tirofiban, eptifibatide, abciximab, melagatran, disulfatohirudin, tissue plasminogen activai or, modified tissue plasminogen activator, anistreplase, urokinase, and streptokinase.

A preferred second agent is at least one anti-platelet agent. Especially preferred anti-platelet agents are aspirin and clopidogrel.

The term anti-platelet agents (or platelet inhibitory agents), as used herein, dénotés agents that inhibit platelet function, for example by inhibiting the aggregation, adhesion or granular sécrétion of platelets. Agents include, but are not limited to, the various known non-steroldal antl-inflammatory drugs (NSAIDS) such as aspirin, ibuprofen, naproxen, sulindac, indomethacin, mefenamate, droxicam, diclofenac, sulfinpyrazone, piroxicam, and pharmaceutically acceptable salts or prodrugs thereof. Of the NSAIDS, aspirin (acetylsalicyclic acid or ASA) and COX-2 inhibitors such as CELEBREX or piroxicam are preferred. Other suitable platelet inhibitory agents include llb/llla antagonists (e.g., tirofiban, eptifibatide, and abciximab), thromboxane-A2receptor antagonists (e.g., ifetroban), thromboxane-A2-synthetase inhibitors, PDE-lll inhibitors (e.g., Pletal, dipyridamole), and pharmaceutically acceptable salts or prodrugs thereof.

The term anti-piatelet agents (or platelet Inhibitory agents), as used herein, is also intended to include ADP (adenosine diphosphate) receptor antagonists, preferably 5 antagonists of the purinergïc receptors P2Y1 and P2Y12·with P2Y12 being even more preferred. Preferred P2Y12 receptor antagonists include ticagrelor, prasugrel, ticlopidine and clopidogrel, including pharmaceutically acceptable salts or prodrugs thereof. Clopidogrel is an even more preferred agent. Ticlopidine and clopidogrel are also preferred compounds since they are known to be gentle on the gastro-intestinal 10 tract in use.

The term thrombin Inhibitors (or anti-thrombin agents), as used herein, dénotés inhibitors of the serine protease thrombin. By inhibiting thrombin, various thrombin-mediated processes, such as thrombin-mediated platelet activation (that is, for example, the aggregation of platelets, and/or the granular sécrétion of plasminogen activator inhibitor-1 and/or serotonin) and/or fibrin formation are disrupted. A number of thrombin inhibitors are known to one of skill in the art and these Inhibitors are contemplated to be used in combination with the présent compounds. Such inhibitors include, but are not limited to, boroarginine dérivatives, boropeptides, dabigatran, heparins, hirudin, argatroban, and melagatran, including pharmaceutically acceptable salts and prodrugs thereof. Boroarginine dérivatives and boropeptides include N-acetyl and peptide dérivatives of boronic acid, such as C-terminal alpha-amïnoboronic acid dérivatives of lysine, omithine, arginine, homoarginine and corresponding Isothiouronium analogs thereof. The term hirudin, as used herein, includes suitable dérivatives or analogs of hirudin, referred to herein as hirulogs, such as disulfatohirudin.

The term thrombolytics or fibrinolytic agents (or thrombolytics or fibrinolytics), as used herein, dénoté agents that lyse blood clots (thrombi). Such agents include tissue plasminogen activator (naturel or recombinant) and modified forms thereof, anistreplase, urokinase, streptokinase, tenecteplase (TNK), lanoteplase (nPA), factor Vlla inhibitors, PAI-1 inhibitors (i.e., inactivators of tissue plasminogen activator inhibitors), alpha2-antiplasmin inhibitors, and anisoylated plasminogen streptokinase activator complex, including pharmaceutically acceptable salts or prodrugs thereof. The term anistreplase, as used herein, refers to anisoylated plasminogen streptokinase activator complex, as described, for example, in EP 028,489, the disdosure of which is hereby incorporated herein by reference herein. The term urokinase, as used herein, is

Intended to dénoté both dual and single chain urokinase, the latter also being referred to herein as prourokinase.

Examples of suitable anti-arrythmic agents include: Class I agents (such as propafenone); Class II agents (such as metoprolol, atenolol, carvadiol and propranolol); Class III agents (such as sotalol, dofetilide, amiodarone, azimilide and ibutilide); Class IV agents (such as ditiazem and verapamil); K+ channel openers such as l^ch inhibitors, and l«ur inhibitors (e.g., compounds such as those disclosed in WO01/40231).

The compounds of the présent invention may be used in combination with antihypertensive agents and such antihypertensive activity is readily determined by those skilled in the art according to standard assays (e.g., blood pressure measurements). Examples of suitable anti-hypertensive agents include: alpha adrenergic blockers; beta adrenergic blockers; calcium channel blockers (e.g., diltiazem, verapamil, nifedipine and amlodipine); vasodilators (e.g., hydralazine), diruetics (e.g., chlorothiazide, hydrochlorothiazide, flumethiazide, hydroflumethiazide, bendroflumethiazide, methylchlorothiazide, trichloromethiazide, polythiazide, benzthiazide, ethacrynic acid tricrynafen, chlorthalidone, torsemide, furosemide, musolimine, bumetanide, triamtrenene, amiloride, spironolactone); renin inhibitors; ACE inhibitors (e.g., captopril, zofenopril, fosinopril, enalapril, ceranopril, cilazopril, delapril, pentopril, quinapril, ramipril, lisinopril); AT-1 receptor antagonists (e.g,, losartan, irbesartan, valsartan); ET receptor antagonists (e.g., sitaxsentan, atrsentan and compounds disclosed in U.S. Patent Nos. 5,612,359 and 6,043,265); Dual ET/AII antagonist (e.g., compounds disclosed in WO 00/01389); neutral endopeptidase (NEP) inhibitors; vasopepsidase inhibitors (dual NEP-ACE inhibitors) (e.g., gemopatrilat and nitrates). An exemplary antianginal agent is ivabradine.

Examples of suitable calcium channel blockers (L-type or T-type) include diltiazem, verapamil, nifedipine and amlodipine and mibefradil.

Examples of suitable cardîac glycosides include digitalis and ouabain.

ln one embodiment, a Formulae I compound may be co-administered with one or more diuretics. Examples of suitable diuretics include (a) loop diuretics such as furosemide (such as LASIX™), torsemide (such as DEMADEX™), bemetanide (such as BUMEX™), and ethacrynic acid (such as EDECRIN™); (b) thiazîde-type diuretics such as chlorothiazide (such as DIURIL™, ESIDRIX™ or HYDRODIURIL™), hydrochlorothiazide (such as MICROZIDE™ or ORETIC™), benzthiazide, hydroflumethiazide (such as SALURON™), bendroflumethiazide, methychlorthiazide, polythiazide, trichlormethiazide, and indapamide (such as LOZOL™); (c) phthalimidinetype diuretics such as chlorthalidone (such as HYGROTON™), and metolazone (such as ZAROXOLYN™); (d) quinazoline-type diuretics such as quinethazone; and (e) potassium-sparing diuretics such as triamterene (such as DYRENIUM™), and amiloride (such as MIDAMOR™ or MODURETIC™).

In another embodiment, a compound of Formulae I may be co-administered with a loop diuretic. In still another embodiment, the loop diuretic Is selected from furosemide and torsemide. In still another embodiment, one or more compounds of Formulae I or II may be co-administered with furosemide. In still another embodiment, one or more compounds of Formulae I or II may be co-administered with torsemide which may optionally be a controlled or modified release form of torsemide.

In another embodiment, a compound of Formulae I may be co-administered with a thiazide-type diuretic. In still another embodiment, the thiazide-type diuretic Is selected from the group consisting of chlorothlazide and hydrochlorothiazide. In still another embodiment, one or more compounds of Formulae I or II may be coadministered with chlorothiazlde. In still another embodiment, one or more compounds of Formulae I or II may be co-administered with hydrochlorothiazide.

In another embodiment, one or more compounds of Formulae I or II may be coadministered with a phthalimidine-type diuretic. In still another embodiment, the phthalimidine-type diuretic is chlorthalidone.

Examples of suitable mineralocorticoid receptor antagonists include sprionolactone and eplerenone.

Examples of suitable phosphodiesterase inhibitors include: PDE III inhibitors (such as cilostazol); and PDE V inhibitors (such as sildenafil).

Those skilled in the art will recognize that the compounds of this invention may also be used in conjunction with other cardiovascular or cerebrovascular treatments including PCI, stenting, drug eluting stents, stem cell therapy and medical devices such as Implanted pacemakers, defibrillators, or cardiac resynchronization therapy.

The dosage of the additional pharmaceutical agent Is generally dépendent upon a number of factors including the health of the subject being treated, the extent of treatment desired, the nature and kind of concurrent therapy, if any, and the frequency 46

V of treatment and the nature of the effect desired. In general, the dosage range of the additional pharmaceutical agent is in the range of from about 0.001 mg to about 100 mg per kilogram body weight of the individual per day, preferably from about 0.1 mg to about 10 mg per kilogram body weight of the Individual per day. However, some variability in the general dosage range may also be required depending upon the âge and weight of the subject being treated, the intended route of administration, the particular anti-obesity agent being administered and the like. The détermination of dosage ranges and optimal dosages for a particular patient is also well within the ability of one of ordinary skill in the art having the benefit of the instant disclosure.

According to the methods of treatment of the invention, a compound of the présent invention or a combination of a compound of the présent invention and at least one additional pharmaceutical agent (referred to herein as a combination'¹) is administered to a subject in need of such treatment, preferably in the form of a pharmaceutical composition. In the combination aspect of the invention, the compound of the présent Invention and at least one other pharmaceutical agent (e.g., another antiobesity agent,) may be administered either separately or in a pharmaceutical composition comprising both. It is generally preferred that such administration be oral.

When a combination of a compound of the présent invention and at least one other pharmaceutical agent are administered together, such administration may be 20 sequential In time or simultaneous. Simultaneous administration of drug combinations is generally preferred. For sequential administration, a compound of the présent invention and the additional pharmaceutical agent may be administered in any order. It ls generally preferred that such administration be oral. It is especially preferred that such administration be oral and simultaneous. When a compound of the présent invention and the additional pharmaceutical agent are administered sequentially, the administration of each may be by the same or by different methods.

According to the methods of the invention, a compound of the présent invention or a combination is preferably administered in the form of a pharmaceutical composition. Accordingly, a compound of the présent invention or a combination can be 30 administered to a patient separately or together in any conventions! oral, rectal, transdermal, parentéral (e.g., intravenous, intramuscular or subcutaneous), intraciste mal, intravaginal, intraperitoneal, topical (e.g., powder, ointment, cream, spray or lotion), buccal or nasal dosage form (e.g., spray, drops or inhalant).

The compounds of the Invention or combinations can be administered alone but will generally be administered in an admixture with one or more suitable pharmaceutical excipients, adjuvants, diluents or carriers known in the art and selected with regard to the intended route of administration and standard pharmaceutical practice, The compound of the invention or combination may be formulated to provide immédiate*, delayed-, modified-, sustained-, pulsed- or controlled-release dosage forms depending on the desired route of administration and the specificîty of release profile, commensurate with therapeutic needs.

The pharmaceutical composition comprises a compound of the invention or a combination in an amount generally in the range of from about 1% to about 75%, 80%, 85%, 90% or even 95% (by weight) of the composition, usually in the range of about 1 %, 2% or 3% to about 50%, 60% or 70%, more frequently in the range of about 1 %, 2% or 3% to less than 50% such as about 25%, 30% or 35%.

Methods of preparing various pharmaceutical compositions with a spécifie amount of active compound are known to those skilled in this art. For examples, see Remington: The Practice of Pharmacy, Lippincott Williams and Wilkins, Baltimore Md.

20.sup.th ed. 2000.

Compositions suitable for parentera! injection generally inciude pharmaceutically acceptable stérile aqueous or nonaqueous solutions, dispersions, suspensions, or émulsions, and stérile powders for reconstitution into stérile injectable solutions or dispersions. Examples of suitable aqueous and nonaqueous carriers or diluents (including solvents and vehicles) inciude water, éthanol, polyols (propylene glycoi, polyethylene glycol, glycerol, and the like), suitable mixtures thereof, triglycérides including vegetable oils such as olive oil, and injectable organic esters such as ethyi oleate. A prefrerred carrier is Miglyol.RTM. brand caprylic/capric acid ester with glycérine or propylene glycol (e.g., Miglyol.RTM. 812, Miglyol.RTM. 829, Miglyol.RTM. 840) available from Condea Vista Co., Cranford, N.J. Proper fluidity can be maintained, for example, by the use of a coating such as lecithin, by the maintenance of the required particle size in the case of dispersions, and by the use of surfactants.

These compositions for parentera! injection may also contain excipients such as preserving, wetting, emulsifying, and dispersing agents. Prévention of microorganism contamination of the compositions can be accompiished with various antibacterial and

W antifungal agents, for example, parabens, chlorobutanol, phénol, sorbic acid, and the like. It may also be désirable to include isotonie agents, for example, sugars, sodium chloride, and the like. Prolonged absorption of injectable pharmaceutical compositions can be brought about by the use of agents capable of delaying absorption, for example, aluminum monostearate and gelatin.

Solid dosage forms for oral administration include capsules, tablets, chews, lozenges, pills, powders, and multi-particulate préparations (granules). In such solid dosage forms, a compound of the présent invention or a combination is admixed with at least one inert excipient, diluent or carrier. Suitable excipients, diluents or carriers

Include materials such as sodium citrate or dicalcium phosphate and/or (a) one or more fillers or extenders (e.g., microcrystalline cellulose (available as Avicel.TM. from FMC Corp.) starches, lactose, sucrose, mannitol, silicic acid, xylitol, sorbitol, dextrose, calcium hydrogen phosphate, dextrin, alpha-cyclodextrin, beta-cyclodextrin, polyethylene glycol, medium chain fatty acids, titanium oxide, magnésium oxide, aluminum oxide and the like); (b) one or more binders (e.g., carboxymethylcellulose, methylcellulose, hydroxypropylcellulose, hydroxypropylmethylcellulose, gelatin, gum arable, ethyl cellulose, polyvinyl alcohol, pullulan, pregelatinized starch, agar, tragacanth, alginates, gelatin, polyvinylpyrrolidone, sucrose, acacia and the like); (c) one or more humectants (e.g., glycerol and the like); (d) one or more disintegrating agents (e.g., agar-agar, calcium carbonate, potato or tapioca starch, alginic acid, certain complex silicates, sodium carbonate, sodium lauryl sulphate, sodium starch glycolate (available as Explotab.TM.from Edward Mendell Co.), cross-linked polyvinyl pyrrolidone, croscarmellose sodium A-type (available as Ac-di-sol.TM.), polyacrilin potassium (an Ion exchange resin) and the like); (e) one or more solution retardera (e.g., paraffin and the like); (f) one or more absorption acceleratora (e.g., quatemary ammonium compounds and the like); (g) one or more wetting agents (e.g., cetyl alcohol, glycerol monostearate and the like); (h) one or more adsorbents (e.g., kaolin, bentonite and the like); and/or (i)one or more lubricants (e.g., talc, calcium stéarate, magnésium stéarate, stearic acid, polyoxyl stéarate, cetanol, talc, hydrogenated caster oil, sucrose estera of fatty acid, dimethylpolysiloxane, microcrystalline wax, yellow beeswax, white beeswax, solid polyethylene glycols, sodium lauryl sulfate and the like). In the case of capsules and tablets, the dosage forms may also comprise buffering agents.

Solid compositions of a similar type may also be used as fillers in soft or hard filled gelatin capsules using such excipients as lactose or milk sugar, as well as high molecular weight polyethylene glycols, and the like.

Solid dosage forms such as tablets, dragees, capsules, and granules may be prepared with coatings and shells, such as enteric coatings and others well known in the art. They may also contain opacifying agents, and can also be of such composition that they release the compound of the présent invention and/or the additional pharmaceutical agent In a delayed manner. Examples of embedding compositions that can be used are polymeric substances and waxes. The drug may also be in microencapsulated form, if appropriate, with one or more of the above-mentioned excipients.

For tablets, the active agent will typically comprise less than 50% (by weight) of the formulation, for exampie less than about 10% such as 5% or 2.5% by weight. The prédominant portion of the formulation comprises fiilers, diluents, disintegrants, lubricants and optionally, flavors. The composition of these excipients is well known in the art. Frequently, the filiers/diluents will comprise mixtures of two or more of the following components: microcrystailine cellulose, mannitol, lactose (ail types), starch, and di-calcium phosphate. The filler/dîluent mixtures typically comprise less than 98% of the formulation and preferably less than 95%, for exampie 93.5%. Preferred disintegrants include Ac-di-sol.TM., Explotab.TM., starch and sodium lauryl sulphate. When présent a disintegrant will usually comprise less than 10% of the formulation or less than 5%, for example about 3%. A preferred lubricant is magnésium stéarate. When présent a lubricant wiil usually comprise less than 5% of the formulation or less than 3%, for example about 1%.

Tablets may be manufactured by standard tabletting processes, for example, direct compression or a wet, dry or melt granulation, melt congealing process and extrusion. The tablet cores may be mono or multi-layer(s) and can be coated with appropriate overcoats known in the art.

Liquid dosage forms for oral administration include pharmaceutically acceptable émulsions, solutions, suspensions, syrups, and élixirs, ln addition to the compound of the présent invention or the combination, the liquid dosage form may contain inert diluents commonly used in the art, such as water or other solvents, solubilizing agents and emulsifiers, as for example, ethyl alcohol, isopropyl alcohol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1,3-butylene glycol, dimethylformamide, oils (e.g., cottonseed oil, groundnut oil, corn germ oil, olive oil, castor oil, sesame seed oil and the like), Miglyole.RTM. (availabie from CONDEA Vista Co., Cranford, N.J.), glycerol, tetrahydrofurfuryl alcohol, polyethylene glycols and fatty acïd esters of sorbitan, or mixtures of these substances, and the like.

Besides such inert diluents, the composition may also include excipients, such as wetting agents, emulsifying and suspending agents, sweetening, flavoring, and perfuming agents.

Oral liquid forms of the compounds of the invention or combinations include solutions, wherein the active compound is fully dissoived. Examples of solvents include ail pharmaceutically precedented solvents suitable for oral administration, particularly those In which the compounds ofthe Invention showgood solubility, e.g., polyethylene glycol, polypropylene glycol, edible oils and glyceryl- and glyceride-based Systems. Glyceryl- and glyceride-based Systems may include, for exampie, the following branded products (and corresponding generic products): Captex.TM. 355 EP (glyceryl trlcaprylate/caprate, from Abitec, Columbus Ohio), Crodamol.TM. GTC/C (medium chain triglycéride, from Croda, Cowick Hall, UK) or Labrafac.TM. CC (medium chain triglyides, from Gattefosse), Captex.TM. 500P (glyceryl triacetate I.e. triacetin, from Abitec), Capmul.TM. MCM (medium chain mono- and diglycerides, fromAbitec), Migyol.TM. 812 (caprylic/capric triglycéride, from Condea, Cranford N.J.), Migyol.TM. 829 (caprylic/capric/succinic triglycéride, from Condea), Migyol.TM. 840 (propylene glycol dicaprylate/dïcaprate, from Condea), Labrafil.TM. M1944CS (oleoyl macrogol-6 glycerides, from Gattefosse), Peceol.TM. (glyceryl monooleate, from Gattefosse) and Maisine.TM. 35-1 (glyceryl monooleate, from Gattefosse). Of particular interest are the medium chain (about C.sub.8 to C.sub.10) triglycéride oils. These solvents frequently make up the prédominant portion ofthe composition, i.e., greater than about 50%, usually greater than about 80%, for exampie about 95% or 99%. Adjuvants and additives may also be included with the solvents principally as taste-mask agents, palatability and flavoring agents, antioxidants, stabilizers, texture and viscosity modifiera and solubilizers.

Suspensions, In addition to the compound of the présent invention or the combination, may further comprise carriers such as suspending agents, e.g., ethoxylated isostearyl alcohols, polyoxyethylene sorbitol and sorbitan esters,

V microcrystalline cellulose, aluminum metahydroxide, bentonite, agar-agar, and tragacanth, or mixtures of these substances, and the like.

Compositions for rectal or vaginal administration preferably comprise suppositories, which can be prepared by mixing a compound of the présent Invention or 5 a combination with suitable non-irritating excipients or carriers, such as cocoa butter, polyethylene glycol or a suppository wax which are solid at ordinary room température, but liquid at body température, and therefore, melt in the rectum or vaginal cavity thereby releasing the active component(s).

Dosage forms for topical administration of the compounds of the présent

Invention or combinations include ointments, creams, lotions, powders and sprays. The drugs are admixed with a pharmaceutically acceptable excipient, diluent or carrier, and any preservatives, buffers, or propellants that may be required.

Many of the présent compounds are poorly soluble in water, e.g., less than about 1 .mu.g/mL. Therefore, liquid compositions in solubilizing, non-aqueous solvents such 15 as the medium chain triglycéride oils discussed above are a preferred dosage form for these compounds.

Solid amorphous dispersions, including dispersions formed by a spray-drying process, are also a preferred dosage form for the poorly soluble compounds of the invention. By solid amorphous dispersion is meant a solid material in which at least a 20 portion of the poorly soluble compound is in the amorphous form and dispersed In a water-soluble polymer. By amorphous Is meant that the poorly soluble compound is not crystalline. By crystalline is meant that the compound exhibits long-range order in three dimensions of at least 100 repeat units in each dimension. Thus, the term amorphous Is intended to include not only material which has essentially no order, but 25 also material which may hâve some small degree of order, but the order is in less than three dimensions and/or is only over short distances. Amorphous material may be characterized by techniques known in the art such as powder x-ray diffraction (PXRD) crystallography, solid state NMR, or thermal techniques such as differential scanning calorimetry (DSC).

Preferably, at least a major portion (i.e., at least about 60 wt %) of the poorly soluble compound in the solid amorphous dispersion is amorphous. The compound can exist within the solid amorphous dispersion in relatively pure amorphous domains or régions, as a solid solution of the compound homogeneously distributed throughout the polymer or any combination of these states or those states that lie intermediate between them. Preferably, the solid amorphous dispersion Is substantially homogeneous so that the amorphous compound is dispersed as homogeneously as possible throughout the polymer. As used herein, substantially homogeneous* means that the fraction of the compound that is présent in relatively pure amorphous domains or régions within the solid amorphous dispersion is relatively small, on the order of less than 20 wt %, and preferably less than 10 wt % of the total amount of drug.

Water-solubie polymers suitable for use in the solid amorphous dispersions should be Inert, in the sense that they do not chemically react with the poorly soluble compound in an adverse manner, are pharmaceutically acceptable, and hâve at least some solubility in aqueous solution at physiologically relevant pHs (e.g. 1-8). The polymer can be neutral or ionizable, and should hâve an aqueous-solubility of at least 0.1 mg/mL over at least a portion of the pH range of 1-8.

Water-soiuble polymers suitable for use with the présent invention may be cellulosic or non-cellulosic. The polymers may be neutral or ionizable in aqueous solution. Of these, ionizable and cellulosic polymers are preferred, with ionizable cellulosic polymers being more preferred.

Exemplary water-solubie polymers include hydroxypropyl methyl cellulose acetate succlnate (HPMCAS), hydroxypropyl methyl cellulose (HPMC), hydroxypropyl methyl cellulose phthalate (HPMCP), carboxy methyl ethyl cellulose (CMEC), cellulose acetate phthalate (CAP), cellulose acetate trimellitate (CAT), polyvinylpyrrolidone (PVP), hydroxypropyl cellulose (HPC), methyl cellulose (MC), block copolymers of ethylene oxide and propylene oxide (PEO/PPO, also known as poloxamers), and mixtures thereof. Especially preferred polymers include HPMCAS, HPMC, HPMCP, CMEC, CAP, CAT, PVP, poloxamers, and mixtures thereof. Most preferred is HPMCAS. See European Patent Application Publication No. 0 901 786 A2_t the disclosure of which is incorporated herein by reference.

The solid amorphous dispersions may be prepared according to any process for forming solid amorphous dispersions that results in at least a major portion (at least

60%) of the poorly soluble compound being in the amorphous state. Such processes inciude mechanical, thermal and solvent processes. Exemplary mechanical processes include milling and extrusion; melt processes including high température fusion, solventmodified fusion and melt-congeal processes; and solvent processes including nonsolvent précipitation, spray coating and spray drying. See, for example, the following

U.S. Patents, the pertinent disclosures of which are incorporated herein by reference: Nos. 5,456,923 and 5,939,099, which describe forming dispersions by extrusion processes; Nos. 5,340,591 and 4,673,564, which describe forming dispersions by milling processes; and Nos. 5,707,646 and 4,894,235, which describe forming dispersions by melt congeal processes, ln a preferred process, the solid amorphous dispersion is formed by spray drying, as disclosed in European Patent Application Publication No. 0 901 786 A2. ln this process, the compound and polymer are dissolved in a solvent, such as acetone or methanol, and the solvent is then rapidly removed from the solution by spray drying to form the solid amorphous dispersion. The solid amorphous dispersions may be prepared to contain up to about 99 wt % of the compound, e.g., 1 wt %, 5 wt %, 10 wt %, 25 wt %, 50 wt %, 75 wt %, 95 wt %, or 98 wt % as desired.

The solid dispersion may be used as the dosage form itself or it may serve as a manufacturing-use-product (MUP) in the préparation of other dosage forms such as capsules, tablets, solutions or suspensions. An example of an aqueous suspension Is an aqueous suspension of a 1:1 (w/w) compound/HPMCAS-HF spray-dried dispersion containing 2.5 mg/mL of compound In 2% polysorbate-80. Solid dispersions for use in a tablet or capsule will generally be mixed with other excipients or adjuvants typically found in such dosage forms. For example, an exemplary filler for capsules contains a 2:1 (w/w) compound/HPMCAS-MF spray-dried dispersion (60%), lactose (fast flow) (15%), microcrystalline cellulose (e.g., Avicel.sup.(R0-102) (15.8%), sodium starch (7%), sodium lauryl sulfate (2%) and magnésium stéarate (1%).

The HPMCAS polymers are available in low, medium and high grades as Aqoa.sup.(R)-LF, Aqoat.sup.(R)-MF and Aqoat.sup.(R)-HF respectively from Shin-Etsu Chemical Co., LTD, Tokyo, Japan. The higher MF and HF grades are generally preferred.

The following paragraphs describe exemplary formulations, dosages, etc. useful for non-human animais. The administration of the compounds of the présent invention and combinations of the compounds of the présent invention with anti-obesity agents can be effected orally or non-orally.

An amount of a compound of the présent invention or combination of a compound of the présent invention with another anti-obesity agent is administered such that an effective dose is received. Generally, a daily dose that is administered orally to an animal is between about 0.01 and about 1,000 mg/kg of body weight, e.g., between about 0.01 and about 300 mg/kg or between about 0.01 and about 100 mg/kg or between about 0.01 and about 50 mg/kg of body weight, or between about 0.01 and about 25 mg/kg, or about 0.01 and about 10 mg/kg or about 0.01 and about 5 mg/kg.

Conveniently, a compound of the présent invention (or combination) can be carried in the drinking water so that a therapeutic dosage of the compound is ingested with the daily water supply. The compound can be directly metered Into drinking water, preferably in the form of a liquid, water-soluble concentrate (such as an aqueous solution of a water-soluble sait).

Conveniently, a compound of the présent invention (or combination) can also be added directly to the feed, as such, or in the form of an animal feed supplément, also referred to as a premix or concentrate. A premix or concentrate of the compound in an excipient, diluent or carrier is more commonly employed for the inclusion of the agent ln the feed. Suitable excipients, diluents or carriers are liquid or solid, as desired, such as water, various meals such as alfalfa meal, soybean meal, cottonseed oil meal, linseed oil meal, comcob meal and corn meal, molasses, urea, bone meal, and minerai mixes such as are commonly employed in poultry feeds. A particulariy effective excipient, diluent or carrier is the respective animal feed itself; that is, a small portion of such feed. The carrier facilitâtes uniform distribution of the compound in the finished feed with which the premix Is blended. Preferably, the compound is thoroughly blended into the premix and, subsequently, the feed. In this respect, the compound may be dispersed or dissolved in a suitable oily vehicle such as soybean oil, corn oil, cottonseed oil, and the like, or in a volatile organic solvent and then blended with the carrier. It wili be appreciated that the proportions of compound in the concentrate are capable of wide variation since the amount of the compound in the finished feed may be adjusted by blending the appropriate proportion of premix with the feed to obtain a desired level of compound.

High potency concentrâtes may be blended by the feed manufacturer with proteinaceous carrier such as soybean oil meal and other meals, as described above, to produce concentrated suppléments, which are suitable for direct feeding to animais, ln 55 such instances, the animais are permitted to consume the usual diet. Altematively, such concentrated suppléments may be added directly to the feed to produce a nutritionally balanced, finished feed containing a therapeutically effective level of a compound of the présent invention. The mixtures are thoroughly blended by standard procedures, such as in a twin shell blender, to ensure homogeneity.

If the supplément is used as a top dressing for the feed, it likewise helps to ensure uniformity of distribution of the compound across the top of the dressed feed.

Drinking water and feed effective for increasing lean méat déposition and for improving lean méat to fat ratio are generally prepared by mixing a compound of the présent invention with a sufficient amount of animal feed to provide from about 10.sub.3 to about 500 ppm of the compound in the feed or water.

The preferred medicated swine, cattle, sheep and goat feed generally contain from about 1 to about 400 grams of a compound of the présent invention (or combination) per ton of feed, the optimum amount for these animais usually being about 50 to about 300 grams per ton of feed.

The preferred poultry and domestic pet feeds usually contain about 1 to about 400 grams and preferably about 10 to about 400 grams of a compound of the présent invention (or combination) per ton of feed.

For parentéral administration in animais, the compounds of the présent invention (or combination) may be prepared in the form of a paste or a pellet and administered as an implant, usually under the skin of the head or ear of the animal in which increase in lean méat déposition and improvement in lean méat to fat ratio is sought.

Paste Formulations may be prepared by dispersing the drug in a pharmaceutically acceptable oil such as peanut oil, sesame oil, com oil or the like.

Pellets containing an effective amount of a compound of the présent invention, pharmaceutical composition, or combination may be prepared by admixing a compound of the présent invention or combination with a diluent such as carbowax, camuba wax, and the like, and a lubricant, such as magnésium or calcium stéarate, may be added to improve the pelletîng process.

It is, of course, recognized that more than one pellet may be administered to an anima! to achieve the desired dose level which will provide the increase in lean méat φ déposition and improvement In lean méat to fat ratio desired. Moreover, implants may also be made periodically during the animal treatment period in order to maintain the proper drug level in the animal's body.

The présent invention has several advantageous veterinary features. For the pet owner or veterinarian who wishes to increase leanness and/or trim unwanted fat from pet animais, the Instant invention provides the means by which this may be accomplished. For poultry, beef and swine breeders, utilization of the method of the présent invention yields leaner animais that command higher sale prices from the méat Industry.

EXAMPLES

Unless specified otherwise, starting materials are generally available from commercial sources such as Aldrich Chemicals Co. (Milwaukee, Wl), Lancaster Synthesis, Inc. (Windham, NH), Acros Organics (Fairlawn, NJ), Maybridge Chemical Company, Ltd. (Comwall, England) and Tyger Scientific (Princeton, NJ). Certain common abbreviations and acronyms hâve been employed which may Include: AcOH (acetic acid), BOP (benzotriazo-1-yloxytris(dimethylamino)phosphonium hexafluorophosphate), DBU (1,S-diazabicyclo[5.4.0]undec-7-ene), CDI (1,1 carbonyldiimidazole), DCM (dichloromethane), DEA (diethylamine), DMAP (4dimethylaminopyridine), DMF (N,/V'-dimethylformamide), DMSO (dimethylsulfoxide),

EDCI (M-(3-dimethylaminopropyl)-/V-ethylcarbodiimide), Et₂O (dïethyi ether), EtOAc (ethyl acetate), EtOH (éthanol), HATU (2-(1H-7-azabenzotriazol-1-yl)-1,1,3,3tetramethyl uranium hexafluorophosphate methanaminium), HBTU (O-benzotriazol-1-ylW,W,W',/V-tetramethyluronium hexafluorophosphate), HOBT(1-hydroxybenzotriazole), IPA (isopropyl alcohol), KHMDS (potassium hexamethyldisilazane), MeOH (methanol),

MTBE (tert-butyl methyl ether), NaBH(OAc)₃ (sodium triacetoxyborohydride), NaHMDS (sodium hexamethyldisilazane), NMP (N-methylpyrrolidone), SEM ([2(Trimethylsilyl)ethoxy]methyl), TFA (trifluoroacetic acid), THF (tetrahydrofuran), and T₃P (propane phosphonic acid anhydride).

Reactions were performed in air or, when oxygen- or moisture-sensitive reagents 30 or intermediates were employed, under an inert atmosphère (nitrogen or argon). When appropriate, reaction apparatuses were dried under dynamic vacuum using a heat gun, and anhydrous solvents (Sure-Seal™ products from Aldrich Chemical Company, • Milwaukee. Wisconsin or DriSolv™ products from EMD Chemicals, Gibbstown, NJ) were employed. Commercial solvents and reagents were used without further purification. When indicated, reactions were heated by microwave irradiation using

Biotage Initiator or Personal Chemistry Emuys Optimizer microwaves. Reaction progress was monitored using thin layer chromatography (TLC), liquid chromatographymass spectrometry (LCMS), high performance liquid chromatography (HPLC), and/or gas chromatography-mass spectrometry (GCMS) analyses. TLC was performed on pre-coated silica gel plates with a fluorescence indicator (254 nm exitation wavelength) and vlsualized under UV light and/or with l₂, KMnÛ4, C0CI2, phosphomolybdic acid, and/or ceric ammonium molybdate stains. LCMS data were acquired on an Agitent 1100 Sériés instrument with a Leap Technologies autosampler, Gemini C18 columns, MeCN/water gradients, and either TFA, formic acid, or ammonium hydroxide modifiera. The column eluent was analyzed using Waters ZQ mass spectrometer scanning in both positive and négative ion modes from 100 to 1200 Da. Other similar instruments were also used. HPLC data were acquired on an Agilent 1100 Sériés instrument using Gemini or XBridge C18 columns, MeCN/water gradients, and either TFA or ammonium hydroxide modifiera. GCMS data were acquired using a Hewlett Packard 6890 oven with an HP 6890 injecter, HP-1 column (12 mx0.2 mmx0.33 pm), and hélium carrier gas. The sample was analyzed on an HP 5973 mass sélective detector scanning from

50 to 550 Da using électron ionization. Purifications were performed by medium performance liquid chromatography (MPLC) using Isco CombiFiash Companion, AnaLogix IntelliFlash 280, Biotage SP1, or Biotage Isolera One instruments and prepacked Isco RediSep or Biotage Snap silica cartridges. Chiral purifications were performed by chiral supercritical fluid chromatography (SFC) using Berger or Thar instruments; ChiralPAK-AD, -AS, -IC, Chiralcel-OD, or -OJ columns; and CO2 mixtures with MeOH, EtOH, IPrOH, or MeCN, alone or modified using TFA or iPrNH2. UV détection was used to trigger fraction collection.

Mass spectrometry data are reported from LCMS analyses. Nuclear magnetic résonance (NMR) spectra were recorded on 400 and 500 MHz Varian spectrometera.

Chemical shifts are expressed in parts per million (ppm, δ) referenced to the deuterated solvent resldual peaks. Analytical SFC data were acquired on a Berger analytical instrument as described above. Optical rotation data were acquired on a PerkinElmer modei 343 polarimeter using a 1 dm cell.

Concentration in vacuo refers to évaporation of solvent under reduced pressure using a rotary evaporator.

Unless otherwise noted, chemical reactions were performed at room température (about 23 degrees Celsius).

The compounds and intermediates described below were named using the naming convention provided with ChemBioDraw Ultra, Version 12.0 (CambridgeSoft Corp., Cambridge, Massachusetts). The naming convention provided with ChemBioDraw Ultra, Version 12.0 are well known by those skilled in the art and it is believed that the naming convention provided with ChemBioDraw Ultra, Version 12.0 generally comports with the IUPAC (International Union for Pure and Applied Chemistry) recommendations on Nomenclature of Organic Chemistry and the CAS Index rules. Unless noted otherwise, ali reactants were obtained commerdally without further purifications or were prepared using methods known in the literature.

Proton nuclear magnetic spectroscopy (¹H NMR) chemical shifts are given in parts per million downfield from tetramethylsilane and were recorded on a Varian Unity 300,400 or 500 MHz (megaHertz) spectrometer (Varian Inc.; Palo Alto, CA). Chemical shifts are expressed In parts per million downfield from tetramethylsilane. The peak shapes are denoted as follows: s, singlet; d, doublet; t, triplet; q, quartet; quin, quintet; m, multiplet; br s, broad singlet. Mass spectrometry (MS) was performed via atmospheric pressure chemical ionization (APCI), electrospray lonization (ESI), eiectron impact ionization (El) or eiectron scatter (ES) ionization sources. Silica gel chromatography was performed primarily using a medium pressure Biotage or ISCO Systems using columns pre-packaged by various commercial vendors including Biotage and ISCO. Microanalyses were performed by Quantitative Technologies Inc. and were within 0.4% of the calculated values.

The terms “concentrated and evaporated” refer to the removal of solvent at reduced pressure on a rotary evaporator with a bath température less than 60°C. The abbreviation “min and “h stand for “minutes and “hours respectively. The term TLC refers to thin layer chromatography, “room température or ambient température means a température between 18 to 25°C, “GCMS” refers to gas chromatography-mass spectrometry, “LCMS refers to liquid chromatography-mass spectrometry, “UPLC refers to ultra performance liquid chromatography and “HPLC refers to high pressure liquid chromatography.

Hydrogénation may be performed in a Parr Shaker under pressurized hydrogen gas, or in Thales-nano H-Cube fiow hydrogénation apparatus at full hydrogen and a flow rate between 1-2 mL/mln at specified température.

Microwave heating of reactions was conducted using a Biotage® Initiator microwave synthesizer.

HPLC, UPLC, LCMS and GCMS rétention times were measured using the following methods:

Method A: Column: Waters Atlantis dC18 4.6x50 mm, 5 pm; Mobile Phase A: 0.05% trifluoroacetic acid in water (v/v); Mobile phase B: 0.05% trifluoroacetic acid in acetonitrile (v/v); Gradient: 95% A/5% B linear to 5% A/95% B in 4.0 minutes, hold at 5% A/95% B for 5.0 minutes; Flow rate: 2.0 mL/minute.

Method B: Column: Waters XBridge C18 4.6x50 mm, 5 pm; Mobile Phase

A: 0.03% ammonium hydroxide In water (v/v); Mobile Phase B: 0.03% ammonium hydroxide in acetonitrile (v/v); Gradient: 95% A/5% B linear to 5% A/95% B in 4.0 minutes, hold at 5% A/95% B to 5.0 minutes; Flow rate: 2.0 mL/minute.

Method C: Column: XBridge C18 2.1 x 50 mm 5pm; Mobile Phase A: 0.0375% trifluoroacetic acid in water; Mobile Phase B: 0.01875% trifluoroacetic acid in acetonitrile; Gradient: Initial - 1 % B, Time (min)/%B: 0.00/1,0.60/5,4.00/100,4.30/1, 4.70/1 ; Flow Rate: 0.8 mL/min; Column Température: 50°C

Method D: Column: XBridge C18 2.1 x 50 mm 5pm; Mobile Phase A: 0.0375% trifluoroacetic acid in water; Mobile Phase B: 0.01875% trifluoroacetic acid in acetonitrile; Gradient: Initial -10% B, Time (min) ! %B: 0.00/10, 0.50/10,4.00/100, 4.30/10,4.70/10; Flow Rate: 0.8 mL/min; Column Température: 50°C

Method E: Column: Zorbax SB-C18 (2.1 x30)mm, 3.5pm; Mobile Phase A: 0.1% formic acid in water; Mobile Phase B: 0.1% formic acid in acetonitrile; Gradient: Initial 2%, Time (min)/%B: 0.00/2,0.40/2,1.60/90,2.9/90, 3.0/2; Flow Rate: 0.8 mL/min; Column Température: 40°C

Method F: Column: AQUITY BEH C-18,2.1 x 50 mm, 1.7 pm; Mobile Phase A: 0.1% formic acid in acetonitrile, Mobile Phase B: 0.1% formic acid in water; Gradient: Time 30 (min)/%B: 0/90, 0.7/90, 2/15,4/15,4.01/90; Flow: 0.5 mL/min.

φ Method G: Column: AQUITY BEH C-18, 2.1 x 50 mm, 1.7 μ m; Mobile Phase A: 0.1% formic acid in acetonitrile; Mobile Phase B: 0.1% formic acid In water; Gradient: Time (min)/%B: 0/90, 0.7/90, 2/55,3/55, 3.8/5, 5.8/5, 6/90; Flow: 0.5 mL/min.

Method H: Column: XBridge C-18 4.6 x 150 mm, 3.5 μπι; Mobile Phase A: acetonitrile; 5 Mobile Phase B: 5 mM ammonium acetate In water; Time(min)/%B: 0/95,1/95,3/5,

10/5,10.5/95; Flow: 0.8 mL/min

Method I: Column: XBridge-C18 4.6 75 mm 3.5 pm; Mobile Phase A: 0.1% formic acid in acetonitrile; Mobile Phase B: 0.1% formic acid in water; Time(min)/%B: 0/90,0.8/90, 1.8/55, 3/5, 6.5/5,7/90; Flow: 0.8 mL/min, Column Température: 40°C

Method J: Column: Symmetry C-18 2.1 x 50 mm 3.5 pm; Mobile Phase A: 0.1% formic acid in acetonitrile; Mobile Phase B: 0.1% formic acid in water; Time (min)/%B: 0/90, 0.5/90, 2/55, 3/55, 3.5/10,6/10, 7/90; Flow: 0.5 mL/min, Column Température: 45°C.

Method K: Column: Discovery C8, 250 x 4.6 mm; Mobile Phase A: methanol; Mobile Phase B: 0.1% formic acid in water; Time (min)/%B: 0/80, 2/80, 8/20,18/20,19/20,

20/80; Flow rate: 1.0 mL/min; Column Température: 40°C.

Method L: Column: Phenomenex Gemlni-NX, 4.6mm x 50mm, C18,3pm, 110 Â; Mobile phase A: 0.1% formic acid in water (v/v); Mobile phase B: 0.1% formic acid in acetonitrile (v/v); Gradient: Initial conditions: A-95%:B-5%, Linear Ramp to A-0%:B100% over 0.0-4.10 min, hold at A-0%:B-100% from 4.10-4.50 min, retum to initial conditions: 4.60-5.0 min; Flow rate: 1.5 mL/minute. Température: 60°C

Method M: Column: Phenomenex Gemini-NX, 4.6mm x 50mm, C18, 3pm, 110 A; Mobile phase A: 0.1% formic acid in water (v/v); Mobile phase B: 0.1% formic acid in acetonitrile (v/v); Gradient: Initial conditions: A-95%:B-5%, Linear ramp from 0.01.90min; hold from 1.90-2.25min; retum to initial conditions 2.25-2.50min.; Flow rate: 1.50 mL/minute. Température: 60°C

Method N: Column: Waters Acquity UPLC BEH, 2.1 mm x 50 mm, C18,1.7pm; Coiumn Température 60°C; Mobile Phase A: 0.1% formic acid in water (v/v); Mobile phase B:

0.1% formic acid in acetonitrile (v/v); Flow-1.25ml/min; Gradient: Initial conditions: A95%:B-5%; hold at initial from 0.0-0.1min; Linear Ramp to A-5%:B-95% over 0.130 0.8mln; hold at A-5%:B-95% from 0.8-0.9min; retum to initial conditions 0.9-1.2min

Method O: Column: 12 m x 0.2 mm; HP-1 Methyl Siloxane, 0.33 pm film, 1.0 mL/min • column flow; 7.6 min: Initial Oven Température 105°C, 0.1 min hold, 30°C/min ramp to

300°C endpoint at 7.6 min; Inlet parameters: front inlet, split 30:1, He, 6 PSI pressure,

250°C injector, 33.9 mL/min total flow; Instrument Agilent 5890 GC Oven with Agilent

5973 Mass Sélective Detector s Method P: Column: Waters Acquity UPLC BEH, 2.1mmx50mm, C18,1.7pm; Column Température: 60°C; Mobile Phase A: 0.1% formic acid in water (v/v); Mobile Phase B: 0.1% formic acid in acetonitrile (v/v); Flow-1.25m1/min; Gradient: Initial conditions: A-95%:B-5%; hold at initial from 0.0-0.1min; Linear Ramp to A-5%:B-95% over 0.1 2.6min; hold at A-5%:B-95% from 2.6-2.95min; retum to initial conditions 2.95-3.0min

Method Q: Column: Phenomenex Kinetex C18 50 x 3.0 mm 2.6 pm; Mobile Phase A: 0.1% Formic acid in water; Mobile Phase B: 0.1% Formic acid in methanol; Gradient: Time (min)/%B: 0.00/0, 0.30/0, 3.00/100, 3.70/100,3.71/0,4.00/0; Flow: 1.0 mL/mîn;

Method R: Column: AQUITY BEH C-18, 2.1 x 50 mm, 1.7pm; Mobile Phase A: 0.1% formic acid In acetonitrile; Mobile Phase B: 0.1% formic acid in water; Time(min)/%B:

0/90, 0.7/90,2/15,4/15, 4.01/90; Flow: 0.5 mL/min

Method S: Column: AQUITY BEH C18 1.7 pm, 2.1 x 50 mm; Mobile Phase A: 0.1% formic acid In acetonitrile; Mobile Phase B: 0.1% formic acid în water; Time(min)/%B: 0/100,1.5/100,4.0/10, 6.0/10,6.01/100; Flow: 0.4 mL/min; Column Température: 40°C

Method T: Column: Acquity BEH C 18(2.1x50) 1.7 pm; Mobile Phase A: 0.1% formic acid in water; Mobile Phase B: 0.1% formic acid In acetonitrile; Time(min)/%B: 0.00/2, 1.30/90,1.55/90,1.56/2; Flow rate: 1.0 mL/min; Column Température: 50°C

Method U: Column: Resteck C18 (2.1 x30) 3.0 pm; Mobile Phase A: 0.1% formic acid in water; Mobile Phase B: 0.1% formic acid in acetonitrile; Time(mln)/%B: 0.00/2,

1.33/90,1.55/90,1.56/2; Flow rate: 1.0 mL/min; Column Température: 25°C

Method V: Column: AQUITY CSH C18 1.7pm 2.1 x 50; Mobile Phase A: 0.1% formic acid in water; Mobile Phase B: 0.1% formic acid in acetonitrile; Time(min)/%B: 0.00/2, 1.3/90,1.55/90,1.56/2; Flow Rate: 1.0 mL/min; Column Température: 50°C

Method W: Column: AQUITY BEH C18, (2.1X50)mm,1.7pm; Mobile Phase A: 0.1% formic acid in acetonitrile; Mobile Phase B: 0.1% formic acid in water; Time(min)/%B: 0/90, 0.7/90, 2/10,4/10,4.01/90; Flow :0.5 ml/min, Column Température: 40°C “ Method X: Column: XBridge C-18 4.6 x 150 mm, 3.5 pm; Mobile Phase A: acetonitrile;

Mobile Phase B: 5 mM ammonium acetate In water; Time (mîn)/%B: 0/95,1/95,3/5,

8/5, 9/95,10/80; Flow: 0.8 mL/min

Method Y: Column: X-Bridge C-18,4.6x150mm,3.5pm. Mobile Phase A: 0.1% formic acid in acetonitrile; Mobile Phase B: 0.1 % formic acid in water; Time(min)/%B: 0/95, 2/95, 4/5, 6.5/5,7/100, 8/100; Flow:0.8mL/min

Method Z: Column: X-Bridge C18 4.6X75mm 3.5pm; Mobile phase A: 0.1% formic acid in acetonitrile, Mobile Phase B: 0.1% formic acid in water; Time(min)/% B: 0/90, 0.8/90 , 2.0/55, 3.0/55,3.5/10, 6.0/10,7/90; Flow :0.8mL/min, Column Temp: 35°C;

Method A1 : Column: X-Bridge C18 4.6X150mm 3.5pm; Mobile phase A: acetonitrile:water (90:10) 0.1% formic acid, Mobile Phase B: water acetonitrile (90:10) 0.1% formic acid; Time(min)/% B: 0/100,1/100, 2.0/50,4/5, 6.5/5,8/100; Flow: 0.8mL/min, Column Temp: 40°C

Method B1: Column: XBRIDGE-C18 4.6X75mm 3.5pm; Mobile phase A: acetonitrile;

Mobile Phase B: 5mM ammonium acetate; Time(min)/%B: 0/95,2/95,3.5/5, 5/5, 6.5/95,7/95; Flow: 0.8mL/min, Column Temp: 40°C;

Method C1 : Column: Symmetry-C18 2.1X50mm 3.5pm; Mobile phase A: 0.1 % formic acid; Mobile Phase B: 0.1% formic acid In water Time(min)/% B: 0/90, 0.5/90,2/55, 3/55, 3.5/10, 6.5/10,7/90; Flow: O.SmL/mîn, Column Temp: 40°C

Method D1: Column: Nucleodurbiowldepore C8,4.6X50mm,5pm; Mobile Phase: A: acetonitrile, Mobile Phase B: 0.1% formic acid in water Time (min)/%B: 0/80, 0.5/80, 1.2/10,5.5/10, 5.8/80,6/80 Flow : 0.7mL/mln; Column Temp: 45°C

Method E1 : Nucleodurbiowldepore C8, 4.6X50mm,5pm; Mobile phase A: acetonitrile; Mobile Phase B: 0.1% formic acid in water Time (min)/%B: 0/80, 0.5/80,1.2/10,

5.5/10,5.8/80, 6/80; Flow: 0.7mL/min; Column Température : 50°C

Method F1: HP-5 (30m x0.32mm x0.25pm) N2=1.2psi, lnj=230°C, Det=250°C, Split=20:1,1.V=3.0pL Programme: 80°C/min/20°C/min/250°C/15min

Method G1 : Column: Zorbax SB-C18 2.1 X30mm,3.5pm; Mobile Phase A: 0.1 % formic acid In acetonitrile; Mobile Phase B: 0.1% formic acid in water Gradient:

Time(min)/%B: 0/90,1/90,2.5/15,4.5715,4.8/90,5/90; Flow :0.6 ml/mln; Température:

40°C

Method H1: Column: Symmetry C18 2.1 x 50 mm 3.5 pm; Mobile Phase A: 0.1% formic acid in acetonitrile; Mobile Phase B: 0.1% formic acid in water; Time (min)/%B:

0/90, 0.5/90,2/55, 3/55, 3.5/10, 6.5/10, 7/90; Flow: 0.5 mL/min; Column Température:

45°C

Method 11: Column: Symmetry RP-184.6x75mm, 3.5um; Mobile Phase A: acetonitrile; Mobile Phase B: 5mM Ammonium acetate in water; Time(min)/%B: 0/80,1/80,2.0/45, 6.3/45, 7.2/10,10/10,10.2/80; Flow: 0.8mL/min; Column Temp: 30°C îo Method J1 : Column: Symmetry RP-18 4.6x75mm, 3.5um; Mobile Phase A: acetonitrile; Mobile Phase B: 5mM Ammonium acetate in Water; Time (min)/%B: 0/80,1/80,1.5/5, 8.5/5, 9/80, 10/80; Flow: 0.7mL/min.

Method K1 : Column: ZORBAX C-18 4.6x50mm, 1.8um; Mobile Phase A: acetonitrile; Mobile Phase B: 5mM Ammonium acetate In Water; Time(min)/%B: 0/80,1.0/80,1.5/5, 15 5.5/5, 6.0/80, 7.0/80; Flow. 0.7mL/min.

Method L1: Column:Symmetry-C18 2.1X50mm 3.5pm; Mobile phase A: acetonitrile; Mobile Phase B: 0.1 % formic acid in water; Time(min)/%B: 0/90, 0.7/90,1.5/10,4/10, 4.5/90, 5/90; Flow: 0.5mL/min, Column Température: 40°C

Method M1: Column: AQUITY BEH C18 1.7pm,2.1X50mm; Mobile Phase A: 0.1% formic acid in acetonitrile, Mobile Phase B: 0.1% formic acid in water; Tlme(min)/%B: 0/100,1.5/100, 4.0/20,4.5/20,5.5/100, 6/100; Flow: 0.4mL/min; Column Température: 40°C

Method N1: Column: AQUITY BEH C-18,2.1 x 50 mm, 1.7 pm; Mobile Phase A: 0.1% formic acid in 90:10 acetonitrile:water; Mobile Phase B: 0.1% formic acid in 90:10 watenacetonitrile; Time (min)/%B: 0/100, 0.7/100,2/55, 3/55, 3.8/5, 5.8/5, 6.0/100; Flow: 0.5 mL/min; Column Température: 40°C

Method 01 : Column: Zorbax Eclipse C18 4.6 x 50mm, 1,8pm; Mobile Phase A:

acetonitrile; Mobile Phase B: 0.1% formic acid in water; Time(mîn)/%B: 0/80, 0.5/80,

1.5/5, 5.5/5, 6/80, 7/80; Flow 0.7 mL/min

Method R1 : Column: Symmetry C-18, 2.1 x 50 mm 5pm; Mobile Phase A: 0.1% formic acid in acetonitrile; Mobile Phase B: 0.1% formic acid in water; Time(min)/%B: 0/90,

1.5/90,2/15, 5.5/15, 6/90,7/90; Flow: 0.5 mL/min, Column Température: 40°C

Method Q1 : Column: X-Bridge C16 4.6x75mm 3.5pm; Mobile Phase A: 0.1% formic acid in acetonitrile:water (90:10); Mobile Phase B: 0,1% formic acid in wateracetonitrile (90:10); Time(min)/%B: 0/90,1/90,2/50, 4/5,7.5/5,8.0/90; Flow: 0.8 mL/min; Column Température: 40°C

Method R1: Column: XBRIDGE-C18 4.6 x 75mm 3.5pm; Mobile Phase A: acetonitrile; Mobile Phase B: 5mM ammonium acetate; Time(min)/%B: 0/100,2/100,2.8/55, 3.2/5, 10 6.8/5, 7.5/100; Flow: 0.8mL/min; Column Température: 40°C.

Method S1 : Column: X-Bridge C18 4.6 x 75 mm 3.5 pm; Mobile Phase A: acetonitrile:water (90:10) 0.1% formic acid; Mobile Phase B: wateracetonitrile (90:10) 0.1 % formic acid; Time(min)/%B: 0/90,1/90,2.0/50,4/5, 6.5/5, 8/90; Flow: 0.8 mL/min; Column Température: 40°C.

Method T1 : Column: XBRIDGE-C18 4.6 X 75 MM 3.5 pM; Mobile Phase A: acetonitrile; Mobile Phase B: 5mM ammonium acetate; Time(min)/%B: 0/100,2/100, 2.8/5, 6.8/5,7.5/100; Flow: 0.8 mL/min, Column Température: 40°C

Method U1: Column: Symmetry-C18 2.1x50mm 3.5 pm; Mobile Phase A: acetonitrile;

Mobile Phase B: 0.1% formic acid in water Time(min)/% B: 0/90,1.5/90,2/15, 5.5/15, 20 6/90, 7/90; Flow: 0.5 mL/min; Column Température: 45°C

Method V1: Column: X-Brdige C18 4.6x75mm 3.5 pm; Mobile Phase A: acetonitrile:water (90:10) 0.1% formic acid; Mobile Phase B: wateracetonitrile (90:10) 0.1% formic acid in water; Time(min)/%B: 0/100,1/100,1.8/50, 3.1/5, 7/5,7.01/100; Flow: 0.8 mL/min; Column Température: 40°C

Method W1 : Column: Χ-Bridge C18 4.6x75mm 3.5pm; Mobile Phase A:

acetonitrile:water (90:10) 0.1% formic acid; Mobile Phase B: wateracetonitrile (90:10) 0.1% formic acid In water Time(min)/%B: 0/100,1/100, 2.0/50,4/5,7.5/5,8/100; Flow: 0.8 mL/mln; Column Température: 40°C

Method X1 : Column: XBridge-C18 4.6x75 mm 3.5 pm; Mobile Phase A: acetonitrile,

Mobile Phase B: 5Mm ammonium acetate; Time(min)/%B: 0/100,2/55,2.8/5,6.8/5, 7.5/100; Flow: 0.8 mL/min; Column Température: 40°C

Method Y1 : Column: X-Brdige C18 4.6x75mm 3.5 gm; Mobile Phase A:

acetonitrile:water (90:10) 0.1% formic acid; Mobile Phase B: wateracetonitrile (90:10)

0.1 % formic acid in water; Time(min)/%B: 0/100,1/100,1.8/50, 3.1/5, 7/5, 7.01/100;

Flow: 0.8 mL/min; Column Température: 40°C.

Method Z1 : Column: Symmetry-C18 2.1x50mm 3.5gm; Mobile Phase a: 0.1 % formic acid in acetonitrile; Mobile Phase B: 0.1% formic acid in water; Time(min)/%B: 0/90, 0.5/90, 2.0/55,3.0/55, 3.5/10,6.0/10,7.0/90; Flow: 0.5 mL/min; Column Température: 45°C

Method A2: Column: Symmetry-C18 2.1x50mm 3.5gm; Mobile Phase A: acetonitrile;

Mobile Phase B: 0.1% formic acid ln water; Time(mîn)/%B: 0/90,1.5/90, 2/15, 4.5/15, 6.5/90,7.0/90; Flow: 0.5 mL/min; Column Température: 45°C

Method B2: Column: Symmetry-C18 2.1x50mm 3.5gm; Mobile Phase A: acetonitrile; Mobile Phase B: 0.1 % formic acid in water; Time(mln)/%B: 0/90,1.5/90, 2/15, 5.5/15, 6.5/90,7/90; Flow: 0.5 mL/mln; Column Température: 45°C

Préparation of Intermediates and Exampies

Intermediate 1 : ffl)-(1-(5,6-Diaminopvridin-2-vl)piperidin-3-vn(pvrrolidin-1-vnmethanone dihvdrochloride

Step 1: (Wert-Butyl 3-(pyrrolidine-1-carbonyl)piperidine-1-carboxylate

To a solution of ('R>1-(fert-butoxycarbonyl)piperidine-3-carboxylîc acid (50.64 g, 220.9 mmol) in anhydrous tetrahydrofuran (552 mL) at 2°C was added 1,1 ’carbonyldiimidazole (77.59 g, 460 mmol). The mixture was stirred at room température for 2 h, cooled to 10 °C and then pyrrolidine (74 mL, 890 mmol) was added slowly. The reaction mixture was stirred at room température for 18 h. The solvent was removed under reduced pressure and water (200 mL) was added to the residue. The mixture was extracted wîth ethyi acetate (2x). The combined organics were washed with aqueous hydrochloric acid (200 mL x 4,1 N) and with a saturated aqueous solution of sodium bicarbonate (200 mL x 2). The organics were dried over magnésium sulfate, filtered and concentrated under reduced pressure to afford (R>tert-butyl 3-(pyrrolidine-

1-carbonyl)piperidine-1-carboxylate as a white solid (58.82 g). The compound was used for the next step without further purification. ¹H NMR (400 MHz. CD₃OD) δ 1.39-1.54 (m, 10H) 1.59-1.65 (m, 1H) 1.67 (d, 1H) 1.69-1.77 (m, 1H) 1.80-2.05 (m, 5H) 2.58 (tt.

H) 2.77 (br s, 1H) 3.33-3.46 (m, 2H) 3.51-3.66 (m, 2H) 3.98-4.12 (m, 2H); MS (AP+) (M+H) 283.3.

Step 2: (R>Piperidin-3-yl(pyrrolidin-1-yl)methanone hydrochloride

To a solution of (fi>tert-butyl 3-(pyrro!idine-1-carbonyl)piperidine-1-carboxylate (58.82 5 g, 208.3 mmol) în anhydrous dichloromethane (100 mL) was added hydrogen chloride

In 1,4-dioxane (260 mL, 1040 mmol, 4M). The reaction mixture was stirred vigorously at room température for 1 h. The solvent was removed under reduced pressure and the residue was left standing ovemight at room température. The residue was triturated with ether (250 mL). The ether was decanted and dichloromethane was added followed 10 by sonîcation in a warm bath. The solvent was removed under reduced pressure and the resulting solid was placed under high vacuum at 40°C for 1 h to afford (RT-piperidin-

3-yl(pyrro!idin-1-yl)methanone hydrochloride (48.41 g). The material was used for the next step without further purification. ’H NMR (400 MHz, CD₃OD) δ 1.75-2.05 (m, ΘΗ) 3.04-3.17 (m, 2H) 3.19-3.28 (m, 3H) 3.35-3.66 (m, 4H); MS (ES+) (M+H) 183.3.

Step 3: (R>(1 -(6-Amino-5-nitropyridin-2-yl)piperidin-3-yl)(pyrro!idin-1 -yljmethanone To a suspension of (R)-piperidin-3-yl(pyrro!idin-1 -yljmethanone hydrochloride (45.56 g, 208.3 mmol) in anhydrous acetonitrile (200 mL) at 10 °C was added triethylamine (50 mL). The suspension was poured into a 2L 3-neck flask equipped with a thermometer 20 and a magnetic stirrer. To the original flask was added anhydrous acetonitrile followed by sonication and addition of triethylamine (80 mL). The suspensions were combined and cooled to 10°C and 6-ch!oro-3-nitropyridin-2-amine (32.97 g, 190 mmo!) was added. The bright yellow solution was stirred under nitrogen while the température was Increased gradually to 80 °C over 1 h. The reaction mixture was cooled to room 25 température. The resulting suspension was filtered and the solids were rinsed with ethyl acetate. The filtrate was concentrated under reduced pressure. A saturated aqueous solution of ammonium chloride (500 mL) was added to the residue and the mixture was extracted into ethy! acetate (2x). The combined organics were washed with brine, dried over magnésium sulfate, filtered, concentrated under reduced pressure 30 and dried for 18 h under high vacuum to afford (R/(1-(6-amino-5-nitropyridin-2yl)piperidin-3-y!)(pyrro!idin-1 -yljmethanone as a yellow foam (63.51 g). ’H NMR (400 MHz, CD₃OD) δ 1.47-1.64 (m, 1H) 1.71-2.07 (m, 7H) 2.68 (tt, 1H) 3.01 (q, 2H) 3.33-3.46

(m, 2H) 3.52 (dt, 1H) 3.60-3.70 (m, 1H) 4.41 (br s, 1H) 4.71 (d, 1H) 6.23 (d, 1H) 8.05 (d,

1H); MS (ES+) (M+H) 320.4.

Step 4: (73)-(1 -(5,6-Diamînopyridin-2-yl)piperidin-3-yl)(pyrrolidin-1 -yl)methanone dihydrochloride

Into a Parr bottle was added 10% palfadium-on-carbon (50% wet, 1487.2 mg) followed by éthanol (10 mL) which was previously bubbled with nitrogen and cooled to 0 °C with an ice-water bath. ((3)-(1-(6-Amlno-5-nitropyridin-2-yl)piperidin-3-yl)(pyrrolidin-1yl)methanone (10.612 g, 33.228 mmol) in éthanol (84 mL) was added into the Parr 10 bottle followed by concentrated hydrochioric acid (8.59 mL, 99.6 mmol) dissolved in éthanol (10 mL) under a nitrogen atmosphère. The reaction mixture was purged with nitrogen and evacuated 3 times. The mixture was submitted to a hydrogen atmosphère (45 PSI). A drop in pressure was obsen/ed and it was increased to 46 PSI. This process was repeated several times over a period of 30 minutes. The réaction mixture 15 was shaken for a total of 1 h. Then it was filtered through Celite and the residue was rinsed with éthanol (1 L). The solvent was removed under reduced pressure and the residue was redissolved in methanol. The solvent was removed under reduced pressure and the solid was dried under high vacuum to afford the title compound (12.0 g). The material was used without further purification. ¹H NMR (400 MHz, CD₃OD) δ 1.67-1.81 20 (m, 2H) 1.83-2.10 (m, 6H) 2.85-2.95 (m, 1 H) 3.32-3.55 (m, 5H) 3.66 (dt, 1 H) 3.89 (d,

1H) 3.97 (dd, 1H) 6.38 (d, 1H) 7.64 (d, 1H); MS (ES+) (M+H) 290.2.

Alternative préparation of ffî>(1-(5,6-diaminopyridin-2-vl)piperidin-3-vl)(pvrTolidin-1vDmethanone

To a solution of ((3>(1-(6-amino-5-nitropyridin-2-yl)piperidin-3-yl)(pyrrofidin-125 yl)methanone (150 mg, 0.47 mmol) in éthanol (20 mL) was added 10% palfadium-oncarbon (100 mg). The réaction mixture was stirred under a hydrogen atmosphère using a balfoon filfed with hydrogen gas for 2 h at room température. The réaction mixture was filtered through Celite and the residue was rinsed with éthanol under a nitrogen atmosphère. The filtrate was used without further purification.

Alternative préparation for /73)-(1-(6-Amino-5-nitropvridin-2-vDpioeridin-3-vl)(ovrrolidin-1vDmethanone (Intermediate 1, Steos 1-3)

Step 1: (73>fert-Butyl 3-(pynOlidine-1-carbonyl)piperidine-1-carboxylate • ÎH>1-(tert-Butoxycarbonyl)piperidine-3-carboxylic acid (6.0 kg, 26 mol) was added in portions to a mixture of Ι,Γ-carbonyldiimidazole (5.1 kg, 31 mol) in tetrahydrofuran (46

L) at a température of 18-22°C. The mixture was held at this température for 3 h, then pyrrolidine (2.28 kg, 32 mol) was added whiie maintaining a température of 18-25°C, and the resulting reaction mixture was held at this température for 2 h. Cyclohexane (48 L) and aqueous potassium carbonate solution (prepared from 4.8 kg potassium carbonate and 48 L water) were then added sequentialiy, and the mixture was stirred for 30 min before séparation of the layers. The organic layer was then washed with another portion of aqueous potassium carbonate solution (prepared from 4.8 kg potassium carbonate and 48 L water). The combined aqueous layers were extracted with cyclohexane (30 L). The combined organic layers were then washed with aqueous sodium chloride solution (prepared from 3.0 kg sodium chloride and 30 L water). The organic layer was dried over sodium sulfate (2.0 kg), then the cyclohexane was removed by distillation under reduced pressure at 45°C. Isopropanol (43 L) was added and the mixture was stirred for 30 min. The presence of (fl)-fert-butyi 3-(pyrrolidine-1 carbonyl)piperidine-1-carboxylate was confirmed by HPLC analysis: HPLC rétention time: 5.98 min (Column: Halo C18,4.6 x 150 mm, 2.7pm; Mobile Phase A: 0.1% orthophosphoric acid and 2% acetonitrile in water, Mobile Phase B: acetonitrile; Gradient: 20%B to 90%B over 7 min, then held for 3 min; Flow: 0.8 mL/min;

Température: 25°C; UV détection at 210 and 226 nM). This material was used in the next step without further purification.

Step 2: ('fî>Piperidin-3-yl(pyrrolidin-1-yi)methanone hydrochloride

Over a period of 1 h, hydrochloric acid (9.8 kg) was added to the solution of (/^-tertbutyl 3-(pyrrolidine-1-carbonyl)piperidine-1-carboxylate in isopropanol from the previous 25 step, maintaining a température of 20-25°C. The reaction mixture was heated to 5055°C and was held at that température for 6 h. Solvent was removed by distillation under reduced pressure at 50°C. Four cycles of soivation and then distillation under reduced pressure at 50°C were conducted sequentialiy: isopropanol (2 x 14.5 L), toluene (18.5 L), and tetrahydrofuran (12.0 L). Another portion of tetrahydrofuran (12.0 30 L) was added and the mixture was stirred at 25-30°C for 1 h. The mixture was centrifuged and the mother liquor was removed; the resulting cake was washed with tetrahydrofuran (3.7 L), then was dried at 40-50°C to afford ffî)-piperidin-3-yl(pyrrolidin-

1-yl)methanone hydrochloride (4.8 kg, 84% over two steps). HPLC rétention time: 1.67 mîn (Column: Halo C18,4.6 x 150 mm, 2.7gm; Mobile Phase A: 0.1% orthophosphoric acid and 2% acetonitrile In water, Mobile Phase B: acetonitrile; Gradient: 20%B to

90%B over 7 min, then held for 3 min; Flow; 0.8 mL/min; Température: 25°C; UV détection at 210 and 226 nM).

Step 3: (H}-(1-(6-Amino-5-nitropyridin-2-ylJpiperidin-3-yl)(pyrrolidin-1-yl)methanone Triethylamine (4.65 kg, 46 mol) was added over a period of 1 h to a mixture of (R)piperidin-3-yl(pyrrolidin-1-yljmethanone hydrochloride (4.8 kg, 22 mol) and acetonitrile (48 L), maintaining a température of 15*20°C. The mixture was held at that température for 30 min, then was warmed to 38-42°C. 6-Chloro-3-nitropyridin-2-amine (3.8 kg, 22 mol) was added portion-wise, and the mixture was held at 38-42°C for 3 h. The mixture was then cooled to 15-20°C and an aqueous solution of ammonium chloride (prepared from 6.24 kg ammonium chloride and 48 L water) and ethyl acetate (48 L) were added sequentlally, and the layers were stirred and then separated. The aqueous layer was further extracted with ethyl acetate (2 x 24 L). The combined organic layers were washed with an aqueous solution of sodium chloride (prepared from 4.8 kg sodium chloride and 24 L water) and then were dried with sodium sulfate (1.92 kg). The organic layer was removed and solvent was evaporated under reduced pressure at 40°C. Ethyl acetate (14.4 L) was added to the crude product, and the mixture was heated to 3540°C and was held at that température for 15 min. The mixture was cooled to 20-25°C and held at that température for 6 h, then cooled to 10-15°C and held at that température for 1 h. The suspension was centrifuged and the mother liquor was removed. The resulting cake was washed with chilled ethyl acetate (4.8 L) and then was dried at 40-50°C to afford ffî>(1-(6-amino-5-nitropyridin-2-yl)piperidin-3yl)(pyrrolidin-1-yljmethanone (4.6 kg, 66%). HPLC rétention time: 5.30 min (Column:

Halo C18,4.6 x 150 mm, 2.7pm; Mobile Phase A: 0.1% orthophosphoric acid and 2% acetonitrile In water, Mobile Phase B: acetonitrile; Gradient: 20%B to 90%B over 7 min, then held for 3 min; Flow: 0.8 mUmin; Température: 25°C; UV détection at 210 and 226 nM). Melting point: 118.5-123.5°C. Water content by Karl Fischer titration: 0.36% by weight.

Intermediate 2: fRM 1 -(4.5-DiamInopvrimidin-2-vl)DiDeridin-3-vl)(Dvrrolidin-1 · yljmethanone dihvdrochloride

Step 1 : (fi)-(1-(4-Amino-5-nitropyrimldin-2-yl)piperidin-3-yl)(pyrrolidin-1 -yljmethanone

A mixture of 2-chloro-5-nitropyrimidin-4-amine (670 mg, 3.64 mmol, 60% pure), piperidin-3-yl(pyrrolidin-1-yl)methanone hydrochloride (prepared by the method described in Steps 1 and 2 of Intermediate 1, 560 mg, 2.56 mmol) and triethylamine (0.761 mL, 5.46 mmol) in dimethylsulfoxide (5 mL) was heated to 100°C for 5 h. The mixture was diluted with ethyl acetate (80 mL), washed with water and brine, dried over sodium sulfate and concentrated. The crude material was purified via flash chromatography (ethyl acetate/heptane:40-70-100%) to afford (R)-(1-(4-amino-5nltropyrimidin-2-yl)piperidin-3-yl)(pyrrolidin-1-yl)methanone as a yellow solid (420 mg). ¹H NMR (500 MHz, DMSO-cfe) δ 1.45 (t, 1 H), 1.62 (d, 1H), 1.72-1.82 (m, 4H), 1.88 10 (d, 4H), 2.56 (br s, 1 H), 2.90-3.04 (m, 2H), 3.28 (t, 2H), 3.37-3.62 (m, 2H), 4.59-4.87 (m,

2H), 8.00 (br s, 1 H), 8.16 (br s, 1H), 8.90 (s, 1H); MS (ES+) (M+H) 321.2.

Step 2: (fi>(1-(4,5-Diaminopyrimidin-2-yl)plperidin-3-yl)(pyrrolidin-1-yl)methanone dihydrochloride

Concentrated hydrochloric acid (470 pL, 37 wt% aq) was added to a solution of (R)-(1 (4-amino-5-nitropyrimidin-2-yl)pîperidin-3-yl)(pyrrolidin-1-yl)methanone (380 mg, 1.13 mmol) in methanol (30 mL). The solution was hydrogenated using the H-Cube (10% Pd/C, full H2,18°C, ImUmin). A second cycle was run using the same conditions. The solvent was removed under reduced pressure and the residue was co-evaporated with toluene several times to afford the titie compound as a yellow solid (405 mg). The material was used without further purification. ¹H NMR (CD₃OD) δ 1.68 (d, 1 H), 1.81 (d, 1H), 1.91 (quin, 3H), 1.96-2.09 (m, 3H), 2.84 (t, 1H), 3.34-3.47 (m, 4H), 3.52-3.59 (m, 1H), 3.61-3.70 (m, 1H), 4.32 (d, 1H), 4.17 (d, 1H), 7.70 (s, 1H); MS (ES+) (M+H)

291.2.

Alternative préparation for (fî)-(1-(4,5-diaminopvrimidin-2-vl)piperidin-3-vl)(pvrrolidin-1vDmethanone

To a stirred solution of (R)-(1-(4-amino-5-nitropyrimidin-2-yl)piperidin-3-yl)(pyrrolidin-1yl)methanone (300 mg, 0.937 mmol) in éthanol (15 mL) was added under a nitrogen 30 atmosphère a suspension of 10% palladium-on-carbon (300 mg) in éthanol. The reaction mixture was hydrogenated using a balloon filled with hydrogen gas for 2 h at room température. The suspension was filtered through Celite and the filtrate was used without further purification.

φ Intermediate 3: 1 -(4-Methvl-1 H-pyrazol-1 -vDcvclopropanecarboxvlic acid

Step 1: tert-Butyl 1-(4-methyl-1H-pyrazol-1-yl)cyclopropanecarboxylate

Into a 25 mL round bottom flask were added sodium hydride (60% dispersion in minerai oil, 164.3 mg, 4.1 mmol) and anhydrous tetrahydrofuran (8 mL). The mixture was cooled with an ice-water bath before a solution of 4-methylpyrazole (204.2 mg, 2.487 mmol) in anhydrous tetrahydrofuran (2 mL) was added. The mixture was stirred in the Ice-water bath for 30 minutes before tert-butyl 2,4-dibromobutanoate (0.48 mL, 2.2 mmol) was added dropwise at 0°C. The reaction mixture was stirred at 0°C for 30 minutes and then at room température for 18 h. The solvent was removed under reduced pressure and water and ethyl acetate were added to the residue. The layers were separated and the aqueous layer was extracted with ethyl acetate (3x). The combined organics were dried over magnésium sulfate, filtered and concentrated under reduced pressure. The crude material was purified via flash chromatography (0-50% ethyl acetate ln heptanes) to afford tert-butyl 1-(4-methyl-1H-pyrazol-115 yl)cyclopropanecarboxylate (218 mg, 44%). ¹H NMR (400 MHz, CDCI3) δ 1.33 (s, 9H), 1.45-1.51 (m, 2H), 1.61-1.67 (m, 2H)H), 1.98-2.01 (m, 3H)H), 7.21 (s, 1 H), 7.23-7.26 (m, 1H).

Step 2: 1 -(4-Methyl-1 H-pyrazol-1 -yl)cyclopropanecarboxylic acid

Into a 100 mL pear shaped flask containing tert-butyl 1-(4-methyl-1 H-pyrazol-120 yl)cyclopropanecarboxylate (218 mg, 0.981 mmol) were added dichloromethane (5 mL) and trifluoroacetic acid (0.2 mL, 3 mmol). The clear solution was stirred at room température for 30 minutes after which another 0.3 mL of trifluoroacetic acid were added. The mixture was stirred at room température for 45 minutes and another 0.3 mL of trifluoroacetic acid were added. The mixture was stirred at room température for 18

h. The solvent and excess of trifluoroacetic acid were removed under reduced pressure to afford the title compound (360 mg). The material was used without further purification. ¹H NMR (400 MHz, CDCI3) δ 1.78 (m, 2H), 2.02 (m. 2H), 2.17 (s, 3H), 7.54 (s, 1H). 7.74 (s, 1H).

Intermediate 4: 1 -(4-Fluoro-1 H-pyrazol-1 -vDcvclooropanecarboxylic acid

Step 1: Benzyl 1-(4-fluoro-1 H-pyrazol-1-yl)cyclopropanecarboxylate

To a solution of 4-fluoropyrazole (125 mg, 1.45 mmol) in tetrahydrofuran (4 mL) at 0°C was added sodium hydride (60% suspension in minerai oil, 116 mg, 2.90 mmol). The mixture was stirred at 0°C for 40 min before benzyl 2,4-dibromobutyrate (0.30 mL, 1.50 mmol) was added dropwise. The reaction mixture was stirred at room température for 1Θ h. The reaction mixture was diluted with ethyl acetate and washed with water and brine. The organlcs were dried over sodium sulfate, filtered and concentrated under reduced pressure. The residue was purified via flash chromatography to afford benzyl

1-(4-fluoro-1 H-pyrazol-1-yl)cyclopropanecarboxylate as a clear oil (115 mg, 30.4%). ’H

NMR (400 MHz, CDCI₃) δ 1.64-1.70 (m, 2H), 1.81-1.86 (m, 2H), 5.14 (s, 2H), 7.22-7.26 (m, 2H), 7.31-7.36 (m, 3H), 7.40 (td, 2H).

Step 2: 1 -(4-Fluoro-1 H-pyrazol-1 -yl)cyclopropanecarboxylic acid

A solution of benzyl 1 -(4-fluoro-1 H-pyrazol-1 -yljcyclopropanecarboxylate (115 mg, 0.442 mmol) in methanol (20 mL) was hydrogenated using the H-Cube (full H2, room température). The solvent was removed under reduced pressure to afford the title compound as a solid (70 mg). MS (AP+) (M+H) 171.4; LCMS rétention time 1.06 minutes (Method M).

Intermediate 5: 1 -(4-Cvano-1 H-pyrazol-1 -vDcvclopropanecarfaoxylic acid

The title compound was prepared by a method anaiogous to Intermediate 4 but using 1 H-pyrazole-4-carbonitrile in Step 1. ’H NMR (400 MHz, CDCI3) δ 1.65 (m, 2H), 1.89 (m, 2H), 7.80 (s, 1H), 8.02 (s, 1H); MS (ES-) (M-H) 176.4, LCMS rétention time: 1.04 25 minutes (Method M).

Intermediate 6: 1 -(4-Cvclopropyl-1 H-pyrazol-1 -vDcvclopropanecarfaoxvlic acid

Step 1: Benzyl 1-(4-bromo-1H-pyrazoi-1-yl)cyclopropanecarboxylate

Benzyl 1-(4-bromo-1 H-pyrazol-1-yl)cyclopropanecarboxylate was prepared by a method anaiogous to Intermediate 4 using 4-bromo-1 H-pyrazole in Step 1. ¹H NMR (400 MHz, 30 CDCh) δ 1.64-1.69 (m, 2H), 1.83-1.88 (m, 2H), 5.14 (s, 2H), 7.21-7.25 (m, 2H), 7.317.38 (m, 3H), 7.50 (d, 1 H), 7.56 (d, 1 H).

Step 2: Benzyl 1-(4-cyclopropyl-1 H-pyrazol-1 -yl)cyclopropanecarboxylate

Into a 20 mL microwave vlal were added benzyl 1 -(4-bromo-1 H-pyrazol-1 yljcyclopropanecarboxylate (421 mg, 1.31 mmol), cyclopropyltrifluoroborate potassium sait (582 mg, 3.93 mmol), 1,1'-Bis(diphenylphosphino)ferrocene]dichloropalladium(ll) complex with dîchloromethane (42.5 mg, 0.052 mmol) and césium carbonate (1710 mg,

5.24 mmol). The vial was sealed, evacuated and backflushed with nitrogen two times followed by the addition of tetrahydrofuran (7 mL). The reaction mixture was stirred at 80°C for 18 h. The solvent was removed under reduced pressure and to the residue was added water (100 mL) and ethyi acetate. The layers were separated and the aqueous layer was extracted with ethyi acetate (2x). After the second extraction an émulsion formed that was cleared with the addition of brine, ethyi acetate and aqueous hydrochloric acid (1N). The aqueous layers were extracted again with ethyi acetate (2x), the solvent was removed under reduced pressure and the residue was purified via flash chromatography (0-100% ethyi acetate in heptanes) to afford benzyl 1-(4-cyclopropyl1H-pyrazol-1-yl)cyclopropanecarboxylate (91 mg, 25%). MS (ES+) (M+H) 283.3;

LCMS rétention time: 3.38 minutes (Method L).

Step 3: 1 -(4-Cyclopropyl-1 H-pyrazol-1 -yljcyclopropanecarboxylic acid

The title compound was prepared by a method anaiogous to Intermediate 4, Step 2. The material was used without further purification. MS (EI+) (M+) 192; GCMS rétention time: 3.096 minutes (Method O).

Intermediate 7: 1-(4-fTrifluoromethvb-1 H-Dvrazol-1-v1)cvcloDroDanecarboxvlic acid

The titie compound was prepared by a method anaiogous to Intermediate 4 but using 4(trifluoromethyi)-l H-pyrazole in Step 1. ¹H NMR (400 MHz, CDCh) δ 1.60-1.79 (m, 2H), 1.83-1.95 (m, 2H), 7.75 (s, 1 H), 7.78-7.89 (m, 1 H), 11.82 (br s, 1 H).

Intermediate 8: 1 -(3-methvlisoxazol-5-v1)cvcloproDanecarboxvlic acid

Stepl: Methyl 1-(3-methylisoxazol-5-yl)cyclopropanecarboxylate

Into a 15 mL round bottom flask was added methyl 2-(3-methylisoxazol-5-yl)acetate (250 mg, 1.61 mmol) dissolved in /V,W-dimethylformamide (7 mL) followed by addition of sodium hydride (60% suspension in minerai oil, 322 mg, 8.06 mmol). The mixture was stirred at room température for 15 minutes. 1,2-Dibromoethane (417 pL, 4.83 mmol) was added dropwise. The reaction mixture was stirred at room température for φ 18 h. A saturated aqueous solution of sodium bicarbonate was added to the mixture and it was extracted with dichloromethane (3x). The combined organics were washed with brine, dried over sodium sulfate, filtered and concentrated under reduced pressure.

The residue was dissolved in dichloromethane (1.5 mL) and washed (3x) with an aqueous (10%) lithium chloride solution to remove residues of Λ/,Ν-dimethylformamide. The organics were dried over sodium sulfate, decanted and concentrated under reduced pressure to afford methyl 1-(3-methy!isoxazo!-5-yl)cyclopropanecarboxy!ate MS (ES+) (M+H) 182.0; LCMS rétention time: 2.53 minutes (Method L). The material was used for the next step without further purification.

Step 2: 1-(3-Methy!isoxazo!-5-y!)cyclopropanecarboxy!ic acid

To methyl 1-(3-methylisoxazol-5-y!)cyclopropanecarboxylate (100 mg, 0.552 mmol) was added a solution of lithium hydroxide monohydrate (32 mg, 0.76 mmol) in methanol (2 mL) and water (1 mL). The reaction mixture was stirred at room température for 18 h. The solvent was reduced to about 1 mL in volume. A saturated aqueous solution of 15 ammonium chloride (2 mL) was added followed by aqueous hydrochloric acid (1 N) until pH was approximately 4. The mixture was extracted with ether (15 mL x 2). The combined organics were dried over sodium sulfate, filtered and concentrated under reduced pressure to afford 1-(3-methylisoxazol-5-yl)cyclopropanecarboxy!ic acid as a white solid (43 mg, 47%). The material was used without further purification. MS (ES+)(M+H) 168.0; LCMS rétention time: 2.0 minutes (Method L). ¹H NMR (500 MHz,

CDjOD) δ 1.44-1.48 (m, 2H), 1.66-1.69 (m, 2H), 2.25 (s, 3H), 6.38 (s, 1 H).

Intermediate 9: 1-(5-Methvlisoxazol-3-vlÎcvclopropanecarboxvlic acid

The title compound was prepared by a method analogous to the one used for

Intermediate 8, but using ethyl 5-methylisoxazo!e-3-carboxylate as the starting material. MS (ES+)(M+H) 168.0; LCMS rétention time: 2.06 minutes. (Method L). ¹H NMR (500 MHz, CDjOD) δ 1.38-1.42 (m, 2H), 1.58-1.62 (m, 2H), 2.39 (s, 3H), 6.32 (s, 1H).

Intermediates 10 and 11:1 -Î3-Methyl-1 H-pyrazol-1 -vDcvclopropanecarboxylic acid and

1-(5-methyl-1 H-pyrazol-1-vDcvclopropanecarboxylic acid

Step 1: tert-Butyl 1-(3-methy!-1H-pyrazol-1-yl)cyc!opropanecarboxylate and tert-butyl 1(5-methyl-1 H-pyrazol-1 -yl)cyclopropanecarboxylate

To a solution of 3-methylpyrazole (306 mg, 3.73 mmol) in tetrahydrofuran (10.0 mL) cooled to 0 °C was added sodium hydride (60% suspension in minerai oil, 242 mg, 6.05 mmol). The mixture was stirred at 0 °C for 40 minutes. tert-Butyl 2,4-dibromobutanoate (0.65 mL, 3.4 mmol) was added dropwise. The reaction mixture was stirred at room température for 2 h. The mixture was diluted with ethyl acetate, washed with water, dried over sodium sulfate, filtered and concentrated under reduced pressure. The residue was purified via flash chromatography (0-25% ethyl acetate in heptanes) to afford a mixture of two regioisomers, tert-butyl 1-(3-methyl-1H-pyrazol-1yl)cyclopropanecarboxylate and tert-butyl 1-(5-methyl-1H-pyrazol-110 yl)cyclopropanecarboxylate (Total weight of mixture: 264 mg).

Step 2: 1-(3-Methyl-1H-pyrazol-1-yl)cyclopropanecarboxylic acid and 1-(5-methyl-1Hpyrazol-1 -yl)cyclopropanecarboxylic acid

To a solution of the regioisomers, prepared in the previous step, (320 mg, 1.44 mmol) in dichloromethane (2 mL) was added trifluoroacetic acid (1.0 mL, 13.0 mmol). The reaction mixture was stirred at room température for 18 h. The solvent was removed under reduced pressure. The residue was purified via HPLC to afford two regioisomers. Regioîsomer 1 (Intermediate 10,69 mg): ¹H NMR (400 MHz, CDCIa) δ 1.33-1.42 (m, 2H), 1.71 (d, 2H), 2.28 (s, 3H), 5.97 (dd, 1H), 7.31 (d, 1H). Regioîsomer 2 (Intermediate 11,82 mg): *H NMR (400 MHz, CDCIa) δ 1.30-1.37 (m, 2H), 1.59-1.66 (m, 2H), 2.22 (s,

3H), 5.96 (d, 1 H), 7.36 (d, 1 H).

Intermediate 12: 1-i1H-pvrazol-1-vl)cvclobutanecarboxvlic acid

Stepl: Ethyl 1-(1H-pyrazol-1-yl)cyclobutanecarboxylate

Into a 50 mL round bottom flask was added sodium hydride (60% dispersion in minerai oil, 452 mg, 11 mmol) and anhydrous tetrahydrofuran (18 mL). To the mixture was added pyrazole (511.6 mg, 7.515 mmol) and anhydrous tetrahydrofuran (1 mL). The mixture was stirred at room température for 2 h and then cooled to 0°C. Ethyl 1bromocyclobutanecarboxylate (1.3 mL, 7.5 mmol) was added dropwise. The reaction mixture was stirred at 0°C for 45 minutes and at room température over three days. HMPA (1.35 mL, 7.5 mmol) was added and the mixture was stirred at room température for 2 h. The réaction mixture was warmed to 70°C for 2 h followed by the addition of Λ/,Ν-dimethylformamide (4 mL). The reaction mixture was stirred at 70°C for another 2 h before more N,A/-dimethylformamide (2 mL) was added and then stirred at 70°C for 18h. The solvent was removed under reduced pressure. To the residue was added water and extracted with a mixture of heptanes and ethyl acetate (3x). The combined organics were dried over magnésium sulfate, filtered and concentrated under reduced pressure. Flash chromatography (0-40% ethyl acetate In heptanes) provided impure ethyl 1-(1H-pyrazol-1-yl)cyclobutanecarboxylate (688mg), which was used without further purification.

Step 2: 1 -(1 H-Pyrazol-1 -yl)cyclobutanecarboxylic acid

To ethyl 1-(1H-pyrazol-1-yl)cyclobutanecarboxylate (688 mg, 3.54 mmol) were added tetrahydrofuran (8 mL) and water (0.9 mL). Aqueous sodium hydroxide (1N, 4 mL, 4 mmol) was added to the mixture. The reaction mixture was stirred at room température for 18h. The solvent was removed under reduced pressure and to the residue was added aqueous hydrochloric acid (1N, 1mL). The mixture was extracted with dichloromethane (3x). The combined organics were dried over magnésium sulfate, filtered and concentrated under reduced pressure to afford the title compound (108.9 mg). The material was used without further purification.

Intermediate 13: Ethyl 1-(pvrimldin-5-vl)cvclopropanecarbimidate hydrochloride

Step 1: 1-(Pyrimidin-5-yl)cyclopropanecarbonitrile

To a mixture of 2-(pyrimldin-5-yl)acetonitrile (104 mg, 0.873 mmol), dibromoethane (113 pL, 1.31 mmol) and tetrabutylammonium bromide (65 mg, 0.23 mmol) dissolved In toluene was added an aqueous solution of sodium hydroxide (50%, 500 mg in 0.5 mL of water, 12.5 mmol). The reaction mixture was stirred at room température for 16 h. The reaction mixture was diluted with ethyl acetate (2 mL), washed with water (0.5 mL x 2) and brine (0.5 mL). The organics were dried over magnésium sulfate, filtered and concentrated under reduced pressure. The residue was purified via flash chromatography (0-80% ethyl acetate in heptanes) to afford 1-(pyrimidin-5yl)cyclopropanecarbonitrile as a solid (28 mg, 22%). ¹H NMR (500 MHz, CDCI₃) δ 1.501.56 (m, 2H), 1.85-1.95 (m, 2H), 8.72 (s, 2H), 9.19 (s, 1H).

Step 2: Ethyl 1-(pyrimidin-5-yl)cyclopropanecarbimidate hydrochloride

1-(Pyrimidin-5-yl)cyclopropanecarbonitrile (28 mg, 0.19 mmol) was suspended Into éthanol (2 mL) previously saturated with gaseous hydrogen chloride. Hydrogen chloride was bubbled for an additional 30 seconds through the mixture and the resulting mixture was stirred for 18h at room température. The solvent was removed under a flow of nitrogen to afford the title compound as a white solid (43 mg) which was used without further purification. ¹H NMR (500 MHz, CD^OD) δ 1.45 (t, 3H), 1.70-1.76 (m, 2H), 1.982.07 (m, 15H), 4.43 (q, 2H), 6.96 (s, 2H), 9.24 (s, 1H).

Intermediate 14: Ethyl 1-(Îsoxazol-3-vl)cvcloDroDanecarbimidate

Step 1: 1 -(lsoxazol-3-yl)cyc!opropanecarbonitrile

To a suspension of sodium hydride (136 mg, 5.39 mmol) in MN-dimethylformamlde (5 mL) was added a solution of 2-(isoxazol-3-yl)acetonitrile (233 mg, 2.16 mmol) in N,N~ dimethylformamide (2.5 mL) at 0 °C. After the addition, the mixture was warmed to room température and stirred for 1 h. The mixture was cooled to 0 °C and 1,210 dibromoethane (0.3 mL, 3.48 mmol) was added. The reaction mixture was stirred at 0 °C for 4 h. Water was added to the mixture. The mixture was extracted with ethyl acetate (3x). The combined organics were washed with water, washed with aqueous hydrochloric acid (1N), washed with aqueous sodium bicarbonate and brine. The organics were dried over sodium sulfate, filtered and concentrated under reduced pressure. The residue was dissolved with dichloromethane and washed with aqueous lithium chloride solution (10%). The organics were dried over sodium sulfate, filtered and concentrated under reduced pressure and dried under a stream of nitrogen to afford 1-(lsoxazol-3-yl)cyclopropanecarbonitrile (243 mg). ’H NMR (500 MHz, CDCI3) δ 1.71-1.76 (m, 2H), 1.79-1.83 (m, 2H), 6.51 (s, 1H), 8.40 (s, 1H). The material was used without further purification.

Step 2: Ethyl 1-(isoxazol-3-yl)cyclopropanecarbimldate hydrochloride

Into an 8 mL vial containing 1-(isoxazol-3-yl)cyclopropanecarbonitrile (243mg, 1.4 mmol) and éthanol (1.98 mL, 34 mmol) was added acetyl chloride (1.45 mL, 20.4 mmol) at 0 °C. The reaction mixture was stirred for 18h at room température. An aliquot was removed and the solvent evaporated; ¹H NMR showed the presence of product. ’H NMR (500 MHz, CDCI₃) δ 1.44 (t, 3H), 1.74-1.79 (m, 2H), 2.16-2.20 (m, 2H), 4.77 (q, 2H), 6.35 (d, 1H), 8.46 (d, 1H). The réaction mixture was concentrated under reduced pressure to afford the title compound (203 mg). The material was used without further purification.

Intermediate 15: Methyi 1-(3-methoxyphenvl)cvcloproDanecarbimidate hydrochloride

Step 1: 1 -(3-Methoxyphenyl)cyclopropanecarbonitrile

To a mixture of 2-(3-methoxyphenyl)acetonitrile (100 mg, 0.679 mmol), 1,2dibromoethane (88 μΙ_, 1.02 mmol) and tetrabutylammomum bromide (50 mg, 0.16 mmol) in toluene (1.0 mL) was added an aqueous solution of sodium hydroxide (50%, 500 mg in 0.5 mL of water, 12.5 mmol) at room température. The reaction mixture was stirred at room température for 16 h. The reaction mixture was diluted with ethyl acetate (2 mL) and washed with water (0.5 mL x 2) and brine (0.5 mL). The organics were dried over magnésium sulfate, filtered, concentrated under reduced pressure and dried under high vacuum. The residue was purified via flash chromatography (0-60% ethyl acetate in heptanes) to afford 1-(3-methoxypheny!)cyclopropanecarbonitrile as an oil (56 mg, 48%). ¹H NMR (500 MHz, CDC!₃) δ 1.38-1.44 (m, 2H), 1.69-1.76 (m, 2H), 3.83 (s, 3H), 6.74-6.96 (m, 3H), 7.22-7.31 (m, 1H).

Step 2: Methyl 1-(3-methoxyphenyl)cyc!opropanecarbimidate hydrochloride 1-(3-Methoxyphenyl)cyclopropanecarbonitrile (56 mg, 0.32 mmol) was dissolved into a saturated solution of hydrogen chloride in éthanol (1mL). The reaction mixture was stored in the refrigerator (4°C) for three days. The reaction mixture was concentrated under reduced pressure and dried under high vacuum. The residue was dissolved in éthanol (0.5 mL) and silica sulfuric acid (125 mg), prepared by following the literature (Tetrahedron, 2001, 57, 9509-9511), was added. The réaction mixture was stirred at room température for 2 h. The silica was filtered off and the filtrate was cooled to 0 °C and gaseous hydrogen chloride was bubbled through the solution for 30 seconds. The reaction mixture was stirred at room température for 18 h. The mixture was concentrated under reduced pressure to give an oil. The oil was dissolved into a solution of hydrogen chloride in methanol (1.25 M, 1 mL). Gaseous hydrochloric acid was bubbled into the solution and the mixture was stirred at room température for 18 h. The réaction mixture was concentrated under reduced pressure to afford the title compound as an oil (40 mg, 51%). The material was used without further purification.

Intermediate 16: Ethyl 1-(f>tolvl)cvclooropanecarbimidate hydrochloride

A saturated solution of hydrogen chloride in éthanol (2 mL) was prepared and was added to 1-(p-tolyl)cyclopropanecarbonitrile (100 mg, 0.64 mmol). The reaction mixture was stirred at room température for 16 h. The température of the reaction mixture was increased to 70°C and the mixture was stirred at that température for 2 h and then allowed to cool to room température. The reaction mixture was left stirring at room température for another 16 h. The solvent was removed under reduced pressure and 79

the residue was dried under high vacuum. The material was used without further purification.

Intermediate 17: Ethyl 2-fethoxvfimino)methvl)-6-methoxyisonicotinate

Step 1: Ethyl 2-chloro-6-methoxyisonicotinate

To a previously sonicated suspension of 2-chloro-6-methoxyisonicotinic acid (4.0g, 21 mmol) in éthanol (50 mL) was added thlonyl chloride (4.64 mL, 64.0 mmol) at 0°C. The reaction mixture was stirred at 0°C for 2 h and then at room température for 18h. Thionyl chloride (4.64 mL, 64.0 mmol) was slowly added and the mixture was stirred at room température for 4 h. The reaction mixture was quenched by slowly pouring into a 10 saturated aqueous solution of sodium bicarbonate (200 mL). Ice was added to the mixture to Iower the température. The mixture was extracted with diethyl ether (150 mL x2). The combined organics were washed with brine, dried over sodium sulfate, filtered and concentrated under reduced pressure to afford a ciear syrup as crude product. The crude product was dried for 2 h under high vacuum while heating to 80°C. The resulting 15 residue was concentrated from toluene (500 mL) to afford ethyl 2-chloro-6methoxyisonicotinate as an off white solid (3.205 g). ¹H NMR (400 MHz, CDCh) δ 1.40 (s, 3H), 3.98 (s, 3H), 4.39 (s, 2H), 7.22 (s, 1H), 7.44 (s, 1H).

Step 2: 2-Cyano-6-methoxyisonicotinîc acid

Into an oven-dried microwave vial was added ethyl 2-chloro-6-methoxyisonicotinate (0.6 20 g, 2.78 mmol), zinc cyanide (392 mg, 3.34 mmol), zinc dust (36.4 mg, 0.2 mmol) and N,N-dimethy!acetamide (10 mL). The vial was capped, evacuated, and filled with nitrogen. This process was repeated 3 times. Then the cap of the vial was removed and bis(tri-t-butylphosphine)palladium(0) (142 mg, 0.278 mmol) was added. The vial was capped again, evacuated, and filled with nitrogen (3x). The reaction mixture was 25 heated to 95°C and left stirring for 18h. The reaction mixture was diluted with ethyl acetate (100 mL) and filtered through Celite. The fiitrate was poured into a saturated aqueous solution of sodium bicarbonate (100 mL). Then the mixture was extracted with ethyl acetate (50 mL x 3). The combined organics were washed with a saturated aqueous solution of ammonium chloride, washed with brine and dried over sodium sulfate. The solvent was removed under reduced pressure and the residue was purified via flash chromatography to afford 2-cyano-6-methoxyisonicotinic acid (260 mg, 45.3%) as a white solid. ¹H NMR (400 MHz, CDCI₃) δ 1.42 (s, 3H), 4.01 (s, 3H), 4.43 (s, 2H), 7.53 (s, 1H), 7.82 (s, 1H).

Step 3: Ethyl 2-(ethoxy(imino)methyl)-6-methoxyisonicotinate

Sodium métal (previously rinsed with heptanes, 10 mg, 0.44 mmol) was added into a flask containing anhydrous éthanol (2.0 mL) cooled with an Ice-water bath. Evolution of gas was observed immediately after addition. The sodium ethoxide formed was added

S to a stirring suspension of 2-cyano-6-methoxyisonicotinic acid (150 mg, 0.727 mmol) in anhydrous éthanol (5.0 mL) at room température under nitrogen. The reaction mixture was sonicated for 5 minutes, stirred at 0°C for 30 minutes and then wanmedwarmed to room température over a period of 30 minutes. GCMS analysis (MS (EI+) (M+) 252;

GCMS rétention time 3.601 minutes, Method O) of the mixture showed conversion of 10 the starting material to the title compound. The reaction mixture was used without further purification.

Intermediate 18: Ethvl 2-(ethoxv(imino)methvl)-6-ethvlisonicotinate

The title compound was prepared by a method analogous to Intermediate 17 but using ethyl 2-chloro-6-ethylisonicotinate as the starting material. GCMS analysis of the reaction mixture showed the presence of the desired product. MS (EI+) (M+) 250; GCMS rétention time 3.611 minutes (Method O).

Intermediate 19: Ethvl 2-cvclopropvlpvrimidine-4-carbimidate

Step 1: 2-Cyclopropylpyrimidine-4-carbonitrile

To a solution of 2-cyclopropylpyrimidine-4-carbaldehyde (29.13 g, 196.6 mmol) in N,Ndimethylformamide (200 mL) was added hydroxylamine hydrochloride (13.9g, 197 mmol) followed by triethylamlne (35 mL, 250 mmol) dissolved in A/./V-dimethylformamide (40 mL) at room température. A 50% solution of propylphosphonic anhydride in N,Ndimethylformamide (140mL, 230 mmol) was added and the reaction mixture was stirred at 110°C for 18 h. The reaction mixture was cooled to room température and a saturated aqueous solution of sodium bicarbonate was added with ethyl acetate. The mixture was stirred vigorously and solid sodium bicarbonate was added. The mixture was filtered to remove undissolved solids and the layers were separated. The aqueous layer was extracted with ethyl acetate (3x) The combined organics were washed with water and brine, dried over sodium sulfate, filtered, and concentrated under reduced pressure, and the resulting residue was dried under high vacuum to afford 281

V cyclopropylpyrimidlne-4-carbonitrile (20.97 g). The material was used without further purification. ¹H NMR (400 MHz, CDCI₃) δ 1.15-1.22 (m, 4H), 7.36 (s, 1H), 8.76 (s, 1H).

Step 2; Ethyl 2-cyclopropylpyrimidine-4-carbimidate

To a solution of 2-cyclopropylpyrimidine-4-carbonitrile (20.97 g, 144,5 mmol) in éthanol (100 mL) was added sodium ethoxide (1.8 g, 78.9 mmol of sodium métal in 30 mL of éthanol). The réaction mixture was stirred at room température for 30 minutes. Diethyl ether (300 mL) was added to the reaction mixture followed by a saturated aqueous solution of ammonium chioride (50 mL). The mixture was extracted with diethyl ether (100 mL x 3). The combined organics were washed with brine (50 mL x 2), dried over magnésium sulfate, filtered, concentrated under reduced pressure and dried under high vacuum to afford the title compound. The material was used without further purification. ¹H NMR (500 MHz, CDCI₃) δ 1.10-1.15 (m, 2H), 1.16-1.22 (m, 2H), 1.43 (t, 3H), 2.32 (tt, 1H), 4.44 (q, 2H), 7.50 (d, 1H), 8.72 (d, 1H).

Intermediate 20: Ethyl 1-(4-(trifluoromethvl)Dhenvl)cvcloDroDanecarbimidate hydrochloride

Into a 4 mL vial was added 1-(4-(trifluoromethyl)phenyl)cyclopropanecarbonitrile (21 mg, 0.1 mmol), éthanol (70 pL) and acetyl chioride (56 pL, 0.792 mmol). The reaction mixture was stirred at room température for 18h. The solvent was removed under reduced pressure to afford the title compound as a white solid (30 mg). The material was used without further purification. ¹H NMR (500 MHz, CDdOD) δ 1.45 (t, 3H), 1.611.66 (m, 2H), 1.95-2.00 (m, 2H), 4.44 (d, 2H), 7.70 (d, 2H). 7.75 (d. 2H).

Intermediate 21: 6-(3-Hydroxvoxetan-3-vl)picolinaldehvde

Step 1: 2-Bromo-6-(1,3-dioxolan-2-yl)pyridine

Te a solution of 6-bromopyridine-2-carbaldehyde (3 g, 16.22 mmol) in toluene (60 mL) was added ethylene glycol (4.97 g, 80.2 mmol) and p-toluenesulphonic acid (0.152 g, 0.8 mmol). The reaction mixture was refluxed for 3 h. The solvent was removed under reduced pressure to afford the crude which was dissolved in ethyl acetate and washed with water. The organic layers were washed with a brine solution, and dried over sodium sulfate. The solvent was removed under reduced pressure to afford 2-bromo-6(1,3-dioxolan-2-yl)pyridine (3.7g). The material was used for the next step without further purification.

• Step 2: 3-(6-(1,3-Dioxolan-2-yl)pyridin-2-yl)oxetan-3-ol

To a solution of 2-bromo-6-(1,3-dioxolan-2-y!)pyridine (2.49 g, 10.8 mmol) in anhydrous tetrahydrofuran (40 mL) was added a solution of n-buty!lithium (2.5 M, 4.3 mL, 10.8 mmol) at -78°C. After stirring for 30 min., a solution of oxetan-3-one (0.6 g, 8.32 mmol) in anhydrous tetrahydrofuran (12 mL) was added slowly and the mixture was stirred at that température for 1 h. The reaction mixture was quenched with water and extracted with ethyl acetate. The organic layer was washed with brine, dried over sodium sulfate, filtered and concentrated under reduced pressure to afford the crude compound. The crude material was purified via préparative TLC eluting with 35 % ethyl acetate in 10 petroleum ether to afford 3-(6-(1,3-dioxo!an-2-yl)pyridin-2-yl)oxetan-3-ol (500 mg, 27%).

MS (ES+) (M+H) 224.20; LCMS rétention time: 2.76 minutes (Method S).

Step 3: 6-(3-Hydroxyoxetan-3-yl)picoiinaidehyde

A solution of 3-(6-( 1,3-dioxoian-2-yl)pyridin-2-yl)oxetan-3-o! (67 mf, 0.30 mmol) and ptoluenesulphonic acid (14.8 mg, 0.26 mmol) in acetone (3.00 mL) was heated to 50°C 15 and stirred for 18h. The reaction mixture was cooied to room température. The solvent was removed under reduced pressure to afford the title compound. The material was used without further purification. MS (ES+)(M+H) 180.0; LCMS rétention time: 1.57 minutes (Method L).

Intermediate 22: Ethyl 1-(3-formvlphenvl)cvclopropanecarboxylate

Step 1: Ethyl 1-(3-bromophenyl)cyclopropanecarboxylate

1-(3-Bromophenyl)cyc!opropanecarboxy!ic acid (976 mg, 4.05 mmol) was dissoived in éthanol (20 mL) and p-toluenesulfonic acid (139 mg, 0.810 mmol) was added. The reaction mixture was heated at reflux for 15 h. The température of the oii bath was 25 increased from 80°C to 110°C and the reaction mixture was stirred for 2 h at that température, after which the mixture was cooled and the solvent was removed under reduced pressure. Tthe crude material was purified via flash chromatography (pentane:ethyl acetate 9:1) to afford ethyl 1-(3-bromophenyl)cyclopropanecarboxylate as a colorless oii (950 mg, 87.2%). ’H NMR (400 MHz, CDCi₃) δ 1.13-1.20 (m, 5H), 30 1.57-1.62 (m, 2H), 4.10 (q, 2H), 7.17 (s, 1H), 7.27 (s, 1H), 7.38 (s, 1H), 7.47-7.50 (m,

1H).

Step 2: (E)-Ethyl 1-(3-(3-ethoxy-3-oxoprop-1-en-1-yi)pheny!)cyciopropanecarboxyiate Ethyl 1-(3-bromophenyl)cyclopropanecarboxylate (740 mg, 2.75 mmol) was dissoived in acetonitrile (20 mL) and the solution was treated with ethy! acrylate (3.90 mL, 35.7 “ mmol) and tri-o-tolyl phosphîne (83.7 mg, 0.275 mmol). The reaction mixture was stirred at room température until ail of the tri-o-tolyl phosphîne had dissolved. Palladium acetate (30.9 mg, 0.137 mmol) was added în a single portion and the reaction mixture was stirred at reflux for 16 hr. Palladium acetate was added (10 mg) and the réaction mixture was stirred for another 4 h. The solvent was removed under reduced pressure and the crude material was purified via flash chromatography (pentane/ethyl acetate 95/5 to 92/8) to afford (£)-ethyl 1-(3-(3-ethoxy-3-oxoprop-1-en-1yl)phenyl)cyclopropanecarboxylate (438 mg, 55%). ¹H NMR (400 MHz, CDCI₃) δ 1.091.19 (m, 5H), 1.31 (t, 3H), 1.56-1.63 (m, 2H), 4.03-4.11 (m, 2H), 4.23 (q, 2H), 6.40 (d,

1 H), 7.20-7.36 (m, 2H), 7.36-7.42 (m, 1 H), 7.44-7.51 (m, 1 H), 7.66 (d, 1 H).

Step 3: Ethyl 1-(3-formylphenyl)cyclopropanecarboxylate

Ethyl 1-(3-bromophenyl)cyclopropanecarboxylate (538 mg, 1.9 mmol) was dissolved in acetone (20 mL). Osmium tetroxide (17.1 pL, 0.07 mmol) and NalÛ4 (1.1 g, 5.6 mmol) were added followed by water (10 mL). The reaction mixture was stirred at room température for 18 h. Water was added (10 mL) followed by pentane (35mL). The layers were separated and the aqueous layer was extracted with pentane (20 mL x 2) and ethyl acetate (20 mL x 2). The combined organics were washed with water, dried over magnésium sulfate, filtered and concentrated under reduced pressure. The residue was purified via flash chromatography (pentane/ethyl acetate 95:5) to afford the title compound as an oil (327 mg, 80%). MS (ES+) (M+H) 219.

Intermediate 23: 3-Benzvloxetane-3-carboxvlic acid

Step 1: 2-Benzyl-2-(hydroxymethyl)propane-1,3-diol

A solution of 3-phenylpropanal (100g, 0.7mol), formaldéhyde (540 mL, 7.5 mol) and calcium oxide (56 g, 1 mol) in éthanol (1 L) was stirred at 40°C under nitrogen for 18 h. The reaction mixture was filtered and the filtrate was concentrated. Water (1L) was added to the residue. The mixture was extracted with ethyl acetate (1L x 3), the combined organic layers were concentrated and purified via silica gel chromatography (petroleum ether/ethyl acetate 3:1) to afford 2-benzyl-2-(hydroxymethyl)propane-1,3-diol as a white solid (90 mg).

Step 2: (3-Benzyloxetan-3-yl)methanol

A solution of potassium f-butoxide in tetrahydrofuran (1M, 24 mL) was added into a solution of 2-benzyl-2-(hydroxymethyl)propane-1,3-diol (90 g, 0.5 mol) and diethyl carbonate (55 g, 0.5 mol) in tetrahydrofuran (500 mL) under nitrogen. The reaction 84 φ mixture was refluxed for 6 h and then heated to 110 °C to distill the solvent. Then the mixture was stirred at 155 °C for 24 h. The mixture was purified via silica gel chromatography (petroleum ether/ethyl acetate 10:1 to 2:1) to afford (3-benzyloxetan-3yljmethanol (40 g, 0.226 mol, 49%) was a white solid.

Step 3: 3-Benzyloxetane-3-carboxylic acid

Jones reagent, (130 mL of a 2.3 M solution) previously prepared by adding hydrochloric acid (46 mL) to water (150 mL) followed by chromium (VI) oxide (55.5 g), was added dropwise to a solution of (3-benzyloxetan-3-yl)methanol (40 g, 0.2 mol) in acetone (600 mL). The reaction mixture was stirred at room température for 5 h. Isopropanol (18 g) 10 was added and the mixture was extracted with ether (4 L), washed with NaH₂PO4 (1M in water, 200 mL) and sodium chloride (1 M in water, 200 mL). The organics were dried over sodium sulfate, filtered and concentrated. The residue was triturated with ether (100 mL) to afford the title compound (37 g, 0.2 mol, 85%) as a white solid. ’H NMR (300 MHz, DMSO-de) δ 3.22 (s, 2H), 4.49 - 4.51 (d, 2H), 4.65-4.67 (d, 2H), 7.12-7.27 15 (m, 5H), 12.85 (s, 1H).

Intermediate 24: ffî)-Î1-(5.6-Diaminopvridin-2-vlÎpiperidîn-3-vD(3.3-difluoropvrrolidin-1vDmethanone dihydrochloride

The title compound was prepared by a method anaiogous to the one used for

Intermediate 1, but using 3,3-difluoropyrrolidine instead of pyrrolidine for Step 1 and a solution of hydrogen chloride in methanol (1.25 M) for Step 2. MS (ES+) (M+H) 326.1 ;

LCMS rétention time: 0.26 minutes (Method M). ¹H NMR (DMSO, 400 MHz): δ 1.45-

1.62 (m, 2H), 1.65-1.72 (m, 1H), 1.82-1.90 (m, 1 H), 2.30-2.45 (m, 2H), 2.76-2.91 (m, 2H), 3.46-3.54 (m, 1H), 3.64-3.78 (m, 2H), 3.84-3.92 (m, 1H), 3.95-4.04 (m, 2H), 4.0625 4.13 (m, 1H), 4.13-4.62 (m, 6H), 6.14 (d, 1H), 7.24 (d, 1H).

I nte rmediate 25: ( (R)-1 -(5.6-Diaminopvridin-2-vDpiperidin-3-vl) ((SÎ-3-hvdroxvpyrrolidîn1-vDmethanone dihydrochloride

The title compound was prepared by a method anaiogous to the one used for

Intermediate 1, but using (S)-pyrrolidin-3-ol instead of pyrrolidine for Step 1 and a hydrogen chloride solution in methanol (1.25 M) for Step 2.. MS (ES+) (M+H) 306.1;

LCMS rétention time: 0.26 minutes (Method M).

W Intermediate 26: 6-(3-(6,7-Dihvdro-5H-pyrrolon .2-c1imidazol-3-vl)piperidin-1 vl)pvridine-2.3-diamine hydrochloride sait

Step 1 : tert-Butyl 3-(5,6,7,7a-tetrahydro-1 H-pyrro!o[1,2-c]imidazo!-3-y!)piperidine-1 carboxylate

To a solution of pyrrolidin-2-ylmethanamine (250 mg, 2.5 mmol) in toluene (8.5 mL) was added 1-tert-butyl 3-ethyl piperidine-1,3-dicarboxylate (635 mg, 2.5 mmol) under nitrogen. The solution was cooied with an ice-water bath and trimethylaluminum (2M in toluene, 2.2 mL, 4.4 mmol) was added. The resulting solution was transferred to a pressure tube and heated to 110°C for 18 h. The reaction mixture was cooied to room température and water was added (10 mL). The mixture was extracted with dichloromethane (40 mL x2). The combined organics were dried over sodium sulfate, filtered, and concentrated under reduced pressure. The residue was purified via flash chromatography (0-12% methanol ln dichloromethane) to afford tert-butyl 3-(5,6,7,7atetrahydro-1 H-pyrrolo[1,2-c]îmidazol-3-yl)piperidine-1 -carboxylate as a colorless oil (317 mg, 43.8%). MS (ES+) (M+H) 294.2; LCMS rétention time: 1.03 minutes (Method M). Step 2: tert-Butyl 3-(6,7-dihydro-5H-pyrrolo[1,2-c]imidazo!-3-yl)piperidine-1-carboxylate To a solution of tert-butyl 3-(5,6,7_f7a-tetrahydro-1 H-pyrro!o[1,2-c]imidazo!-3y!)piperidine-1 -carboxylate (175 mg, 0.6 mmol) in toluene (5 mL) was added BaMnÛ4 (2.1 g, 18 mmol) and the reaction was heated at 115°C for 42h. The reaction was cooied to room température and BaMnCl» (2 g) was added followed by toluene (2 mL). The reaction mixture was heated to 115°C for 3 days. The mixture was cooied to room température and filtered through Celite followed by rinsing with dichloromethane. The filtrate was concentrated under reduced pressure and the residue was purified via HPLC to afford tert-butyl 3-(6,7-dihydro-5H-pyrrolo[1,2-c]imidazo!-3-y!)piperidine-1 25 carboxylate as an oil (25 mg, 14%). MS (ES+) (M+H) 292.2; LCMS rétention time: 1.69 minutes (Method M).

Step 3: 3-(Piperidin-3-yl)-6,7-dihydro-5H-pyrro!o[1,2-c]imidazole trifluoroacetate f-Butyl 3-(6,7-dihydro-5H-pyrrolo[1,2-c]imidazo!-3-yl)pîperidine-1-carboxylate (65 mg,

0.1 mmol) was dissolved Into a 20% trifluoroacetic acid in dichloromethane solution (2.4 mL). The reaction mixture was stirred at room température for 2.5 h. The soivent was removed under reduced pressure to afford 3-(piperidin-3-yl)-6,7-dihydro-5H-pyrrolo[1,2cjimidazole trifluoroacetate sait as an oil (94 mg, 100%). The material was used without further purification.

Step 4: 6-(3-(6,7-Dihydro-5H-pyrrolo[1,2-c]imidazol-3-yl)piperidin-1 -yl)-3-nitropyridin-2amine

To a solution of 3-(piperidin-3-yl)-6,7-dihydro-5H-pyrrolo[1,2-c]imidazole trifluoroacetate sait (94 mg) in anhydrous acetonitrile (1.1 mL) at 10°C was added triethylamine (130 pL, 0.93 mmol). Then 6-chloro-3-nitropyridin-2-amine (39 mg, 0.2 mmol) was added.

The reaction mixture was shaken at 80°C for 2 h and then for 18 h at room température. The solvent was removed under reduced pressure and the residue was dissoived in dichloromethane (3 mL). The mixture was washed with a saturated aqueous solution of sodium bicarbonate (1 mL), washed with water (1 mL), washed with brine (1 mL), dried over sodium sulfate, fiitered and concentrated under reduced pressure. The residue was purified via flash chromatography (0-10% methanol in dichloromethane) to afford 6-(3-(6,7-dihydro-5H-pyrrolo[1,2-c]imidazol-3-yl)piperidin-1 -yl)-3-nitropyridin-2-amine as a yellow oil (60 mg, 82%). MS (ES+) (M+H) 329.2; LCMS rétention time: 1.61 minutes (Method L).

Step 5: 6-(3-(6,7-Dihydro-5H-pyrrolo[1,2-c]imidazol-3-yl)piperidin-1-yl)pyridine-2,3diamine hydrochloride sait 6-(3-(6,7-Dihydro-5H-pyrrolo[1,2-c]imidazol-3-yl)piperidin-1-yl)-3-nitropyridin-2-amine (20 mg, 0.1 mmol) was dissoived in éthanol (0.5 mL) and water (0.1 mL) under nitrogen. Then iron 200 mesh (27 mg, 0.5 mmol) and calcium chloride (3.3 mg, 0.03 mmol) were added and the mixture was heated to 70°C for 18 h. The reaction mixture was cooled to room température followed by filtration through a 0.2 pm nylon syringe filter and rinsing with éthanol (0.5 mL) to afford the title compound. The filtrate was carried on to the next step without further purification. MS (AP+) (M+H) 299.1; LCMS rétention time: 0.44 minutes (Method L)

Intermediate 27: 1-(3-Oxomorpholino)cvclopropanecarboxv1ic acid

The title compound was prepared by a method analogus to the one used for Intermediate 4, but using morpholin-3-one in Step 1. MS (ES+) (M+H) 186.3; LCMS rétention time: 0.59 minutes (Method M).

Intermediate 28: 6-(1 •((tert-ButvldimethvlsilvDoxv)cvclooropvl)picolinonitri1e

Step 1: 2-Bromo-6-(1-((tert-butyldimethy1silyl)oxy)vinyl)pyridine

To a solution of 1-(6-bromopyridin-2-yl)ethanone (1000 mg, 5.0 mmol) and triethylamine (2.1 mL, 15.0 mmol) at 0°C in dichloromethane (14 mL) was added trifluoromethyl tert87

W butyldimethylsilanesulfonate (1.4 mL, 6.2 mmol). The reaction mixture was stirred at room température for 2 h. The réaction mixture was diluted with ethyl acetate, washed with water and brine, dried over sodium sulfate, and concentrated. The residue was purified via flash chromatography (0-30 % ethyl acetate in heptanes) to afford 2-bromo5 6-(1-((tert-butyldimethylsilyl)oxy)vinyl)pyridine as a colorless oil (1450 mg, 92%). ’H

NMR (400 MHz, DMSO-d_e) δ 0.22 (s, 6H), 0.93 (s, 9H), 4.62-4.63 (m, 1H), 5.52 (s, 1H), 7.56-7.62 (m, 2H), 7.82 (s, 1H).

Step 2: 2-Bromo-6-(1 -((fert-butyldimethylsilyl)oxy)cyclopropyl)pyridine

To a solution of diethylzinc (1M in hexane, 2.0 mL, 2.0 mmol) in dichloromethane (3.7 mL) at -4°C was added chloroiodomethane (714 mg, 4.0 mmol) dissolved in dichloromethane (1 mL) dropwise. The reaction mixture was stirred at 0°C for 15 minutes. A solution 2-bromo-6-(1-((tert-butyldimethylsilyl)oxy)vinyl)pyridine (200 mg, 0.6 mmol) in dichloromethane (3.0 mL) was added. The reaction mixture was stirred at 0 °C for 2h. A cold saturated aqueous solution of ammonium chloride was added and the mixture was extracted with dichloromethane. The organics were dried over sodium sulfate and concentrated under reduced pressure. The crude material was purified via flash chromatography (100% heptanes) to afford 2-bromo-6-(1-((fertbutyldimethylsilyl)oxy)cyclopropyl)pyridine (120 mg, 58%). ’H NMR (400 MHz, CDCIg) δ 0.11 (s, 6H), 0.95 (s, 9H), 1.22-1.28 (m, 2H), 1.39-1.45 (m, 2H), 7.23 (dd, 1H), 7.47 (t,

1H), 7.60 (dd, 1H).

Step 3: 6-(1 -((tert-Butyldimethylsilyl)oxy)cyclopropyl)picolinonitrile

Into a vial was added 2-bromo-6-(1-((tert-butyldimethylsilyl)oxy)cyclopropyl)pyridine (60.0 mg, 0.2 mmol), zinc acetate (2.8 mg, 0.01 mmol), zinc dust (3.6 mg, 0.1 mmol), (1,r-bis(diphenylphosphino)ferrocene)-dichloropalladium (II) (2.9 mg, 0.004 mmol) and 25 zinc cyanide (14.0,0.1 mmol). The solids were purged with nitrogen and then N,Ndimethylformamide (0.7 mL) and water (0.1 mL) were added. The suspension was heated to 100°C for 2 h. The reaction mixture was directly loaded onto a silica gel column and purified via flash chromatography (0*5% ethyl acetate in heptanes) to afford the titie compound (45 mg, 90%). ’H NMR (400 MHz, CDCI3) δ 0.10 (s, 6H), 0.96 (s, 30 9H), 1.27-1.33 (m, 2H), 1.43-1.48 (m, 2H), 7.46 (s, 1 H), 7.73-7.80 (m, 1 H), 7.89 (s, 1 H).

Intermediate 29: ffl>-(4-(5,6-DÎamÎnopvridÎn-2-vnmorpholin-2-vn(pvrrolidin-1 yljmethanone dihvdrochloride

The title compound was prepared by a method anaiogous to the one used for Intermediate 1, but using (R)-4-(tert-butoxycarbonyl)morpholine-2-carboxylic acid for Step 1.¹H NMR (400 MHz, CD₃OD) δ 1.81-2.03 (m, 4H) 3.37-3.51 (m, 3H) 3.60-3.73 (m, 3H) 3.75-3.85 (m, 2H) 3.90-3.97 (m, 2H) 4.49 (dd, 1H) 6.40 (d, 1H) 7.73 (d, 1H).

Intermdiate 30: Ethyl 6-cvcloproDvlDvrazine-2-carbimidate

Step 1: 6-Cyclopropylpyrazine-2-carbonitrile

A suspension of 6-cyano-2-chloropyrazine (0.3 g, 2.1 mmol), (1,1’bis(dtphenylphosphino)ferrocene)-dichloropalladium (II) (153.6 mg, 0.21 mmol), potassium phosphate (1.3 g, 6.30 mmol) and cyclopropylboronic acid (0.184 mg, 2.1 mmol) in tetrahydrofuran (25 mL, nitrogen bubbled) was degassed for 15 min. using nitrogen.. The reaction mixture was refluxed for 16 h. The reaction mixture was filtered through Celite and the filtrate was concentrated under reduced pressure. The crude material was purified via column chromatography (100-200 mesh silica gel, 5-20% ethyl acetate in petroleum ether) to afford 6-cyclopropylpyrazine-2-carbonitrile (250 mg, 50%). Step 2: Ethyl 6-cyclopropylpyrazine-2-carbimidate

Sodium ethoxide (140mg, 2.06 mmol) was added to a solution of 6cyclopropylpyrazine-2-carbonitrile ( 150 mg, 1.03 mmol) in éthanol (6 mL) at room température. The reaction mixture was stirred at room température for 1 h. The solvent was removed under reduced pressure. The residue was partitioned between water and ethyl acetate. The organics were dried over sodium sulfate and concentrated under reduced pressure to afford the title compound (250 mg). MS (ES+) (M+H) 192.1012

Intermediate 31 : (R)-(4-(6-amino-5-nitropvridÎn-2-vlÎmorpholin-2-vl)(pvrrolidÎn-1 vDmethanone

The title compound was prepared by a method anaiogous to Intermediate 1, Steps 1,2 and 3, but using (fî)-4-(tert-butoxycarbonyl)morpholine-2-carboxylic acid as the starting material. ¹H NMR (400 MHz, CDaOD) δ 1.82-2.05 (m, 5H), 3.45 (s, 2H), 3.55-3.77 (m, 4H), 3.94-4.05 (m, 1H), 4.26 (s, 2H), 4.58 (s, 1H), 6.28 (s, 1H), 8.16 (s, 1H).

Intermediate 32: 3-NÎtro-6-(2-(ovridin-2-vl)morpholino)pyridin-2-amine

To a solution of 2-(pyridin-2-yl)morpholine hydrochloride (1000 mg, 4.22 mmol) and triethylamine (1.3 mL, 9.3 mmol) in dimethylsulfoxide (10 mL) was added 6-chloro-3nitropyridin-2-amine (734 mg, 4.23 mmol). The reaction mixture was stirred at 100°C for 5 18 h. The réaction mixture was cooled to room température. Ethyl acetate was added and the mixture was washed with water (20 mL). The aqueous layer was extracted with ethyl acetate (20 mL x 2). The combined organics were washed with brine (20 mL), dried over magnésium sulfate, filtered and concentrated under reduced pressure. The crude material was purified via flash chromatography (40-100% ethyl acetate in 10 heptanes) to afford the title compound (0.897 g, 70%). ¹H NMR (400 MHz, CDCI₃) δ

3.08 (s, 1H), 3.18-3.29 (m, 1H), 3.83 (s, 1H), 4.20 (s, 1H), 4.41 (d,1 H), 4.63 (dd, 1H), 4.67-4.80 (m, 1H), 6.18 (s, 1H), 7.23-7.26 (m, 1H), 7.54 (s, 1H), 7.75 (s, 1H), 8.22 (s, 1H), 8.60 (s, 1H).

Intermediate 33: 2-Cvclobutvlpyrimidine-4-carbaldehvde

Step 1: 4-(Dimethy!amino)-1,1 -dimethoxybut-3-en-2-one

A mixture of 1,1-dimethoxy-W,W-dimethylmethanamine (5.0 g, 41.96 mmol) and 1,1dimethoxypropan-2-one (5.0 g, 41.96 mmol) was heated to 80°C for 16 h. The solvent was removed under reduced pressure to afford 4-(dimethylamîno)-1,1-dimethoxybut-3en-2-one as a black colored Iiquid. The material was used without further purification.

Step 2: Cyclobutanecarboximidamide hydrochloride

Hydrogen chloride gas was bubbied through a solution of cyclobutanecarbonitriie (2.0 g, 24.65 mmol) in methanol (10 mL) and diethyi ether (12 mL) for 2 h at 0°C. The reaction mixture was concentrated under reduced pressure and the residue was dissolved in methanol and cooled to 0°C. Methanoiic ammonia was added and the reaction mixture 25 was stirred for 1 h at room température. The solvent was removed under reduced pressure to afford cyclobutanecarboximidamide hydrochloride (3.0 g, 92%). The material was used for the next step without further purification.

Step 3: 2-Cyclobutyl-4-(dimethoxymethyl)pyrimidine

To a solution of 4-(dimethylamino)-1_t1-dimethoxybut-3-en-2-one (2.0 g, 11.5 mmol) in éthanol was added cyclobutanecarboximidamide hydrochloride (3.0 g, 22.3 mmol) and triethylamine (2.33 g, 23.0 mmol) under nitrogen at room température. The reaction mixture was refluxed for 16 h. The solvent was removed under reduced pressure. To the residue was added water and the mixture was extracted with ethyi acetate. The combined organics were dried over sodium sulfate and concentrated under reduced pressure. The crude material was purified via column chromatography to afford 2cyclobutyl-4-(dimethoxymethyl)pyrimidine as a colorless liquid (300 mg, 12%). MS (ES+APCI) (M+H) 209.2; LCMS rétention time: 3.257 minutes (Method J).

Step 4: 2-Cyclobutylpyrimidine-4-carbaldehyde

A solution of 2-cyclobutyl-4-(dimethoxymethyl)pyrimïdine (300 mg, 1.4 mmol) in aqueous hydrochloric acid (3N, 5 mL) was heated to 50°C for 16 h. The reaction mixture was cooled to room température, then was diluted with water, neutralized with a saturated aqueous solution of sodium bicarbonate and extracted with ethyi acetate. The combined organics were dried over sodium sulfate and concentrated under reduced pressure to afford the title compound as a brown colored liquid (200 mg). MS (ES+) (M+H) 163.16.

Intermediate 34: 2-Cvclopropyloxazole-4-carbaldehvde

Step 1: 2-Cyclopropyl-N-methoxy-N-methyloxazole-4-carboxamide

To a solution of 2-cyclopropyloxazole-4-carboxylic acid (0.1 g, 0.653 mmol ) in dry dichloromethane (15 mL) were added 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (0.25 g, 1.3 mmol) and hydroxybenzotriazole (0.175 g, 1.3 mmol) at 0°C portion wise. N,Odimethylhydroxylamine (64 mg, 0.653 mmol) was then added followed by triethylamine (0.26 mL, 1.95 mmol). The reaction mixture was stirred at room température for 18h then diluted wîth ethyi acetate and washed with water and brine solution. The organic layer was dried over sodium sulfate and concentrated under reduced pressure. The crude compound was purified by préparative TLC (25% ethyi acetate in petroleum ether) to afford 2-cyclopropyl-N-methoxy-N-methyloxazole-4carboxamide (100 mg, 83%). MS (ES+) (M+H) 197.25; LCMS rétention time: 2.82min (Method S).

^w Step 2: 2-Cyclopropyloxazole-4-carbaldehyde

To a solution of 2-cyclopropyl-N-methoxy-N-methyloxazole-4-carboxamide (0.102 g,

0.52 mmol) in dry dichloromethane (15 mL) was added diisobutylaluminium hydride (1M in toluene ) (1.04 ml, 1.04 mmol) at -78°C. The reaction mixture was stirred at -78°C for 45 minutes. The reaction mixture was quenched with aqueous sodium hydroxide (2M) and extracted with ethyl acetate. The organic layer was dried over sodium sulfate and concentrated under reduced pressure to afford 2-cyclopropyloxazole-4carbaldehyde which was used without further purification. (65 mg) MS (API-ES+) (M+H)

138.2.

Intermediate 35: 6-(Azetidin-1-vl)Dicolinaldehvde

Step 1: 2-(Azetîdin-1-yl)-6-bromopyridine

To a solution of 2,6-dibromopyridine (100 mg, 0.424 mmol) in dimethylsulfoxide (5 mL) was added potassium carbonate (175.4 mg, 1.27 mmol) and azetidine hydrogen chloride (24.2 mg, 0.424 mmol) at room température. The reaction mixture was heated to 80°C for 16 h, then was poured into ice cold water and extracted with ethyl acetate. The combined organic layers were dried over sodium sulfate, and concentrated under reduced pressure to afford 2-(azetidin-1-yl)-6-bromopyridine (30 mg)

Step 2: 6-(Azetidin-1-yl)picolinaldehyde

A solution of n-butyllithium in hexanes (0.3 g, 4.69 mmol) was added dropwise to a solution of 2-(azetidin-1-yl)-6-bromopyridine (0.5 g, 2.34 mmol) in dry tetrahydrofuran (20 mL) at 78°C . After stirring for 30 min at -78°C, Λ/,Μ-dimethylformamide (0.34 g, 4.69 mmol) was added. The reaction mixture was stirred for 3 h at -78°C, then the reaction mixture was quenched with an aqueous ammonium chloride solution and extracted with ethyl acetate. The organic layers were dried over sodium sulfate, filtered and concentrated under reduced pressure. The crude material was purified via flash chromatography (60-120 mesh silica; 45% ethyl acetate In petroleum ether, twice) to afford 6-(azetidin-1-yl)picolinaldehyde (0.2 g, 43%). MS (ES+) (M+H) 163.1380; LCMS rétention time: 5.59 mininutes (Method X).

Intermediate 36: 6-(Difluoromethoxv)picolinaldehvde

To a solution of 6-hydroxypicolinaldehyde (2 g, 16.24 mmol) in acetonitrile (20 mL) was added sodium 2-chloro-2,2-difluoroacetate (4.46 g, 29.24 mmol) at room température.

The reaction mixture was heated at 70°C for 18h. The reaction mixture was cooled to room température and the solid was filtered off. Then the crude material was purified via flash chromatography (100-200 mesh silica gel, 10% ethyl acetate in hexane) to afford the title compound (1.2g, 42.85%). MS (ES+) (M+H) 174.10

Intermediate 37: 6-(Difluoromethvl)Dicolinaldehyde

Step 1: 6-(1,3-Dioxolan-2-yl)picolinaldehyde

To a solution of pyridine-2,6-dicarbaldehyde (5 g, 37.4 mmol) in benzene (100 mL) was added ethylene glycol (1.1 g, 18.5 mmol) and p-toluenesulfonlc acid (0.3 g, 1.8 mmol). The reaction mixture was equipped with a dean stark condenser and refluxed for 15 min. The reaction mixture was concentrated under reduced pressure and the resulting crude was purified via column chromatography to afford 6-(1,3-dioxolan-2yljpicolinaldehyde (2 g, 30%). MS (ES+) (M+H) 180.0495

Step 2: 2-(Difluoromethyl)-6-(1,3-dioxolan-2-yl)pyridine

To a solution of 6-(1,3-dioxolan-2-yl)plcolinaldehyde (2 g, 11.1 mmol) in chloroform (50 mL) was added diethylaminosulfur trifluoride (DAST) (2 mL) at 0°C. The reaction mixture was stirred at room température for 12 h. The mixture was then concentrated under reduced pressure, diluted with ice cold water and extracted with ethyl acetate. The combined organic layers were dried over sodium sulfate, filtered, concentrated and purified via flash chromatography to afford 2-(difluoromethyl)-6-(1,3-dioxolan-2yljpyridîne (1.62 g, 71%). MS (ES+APCI) (M+H) 202.1; LCMS rétention time: 2.876 min (Method H1).

Step 3: 6-(Difluoromethyl)picolinaldehyde

To a solution of 2-(difluoromethyl)-6-(1,3-dioxolan-2-yl)pyridine (0.5 g, 2.4 mmol) was added 85% formic acid (5 mL). The reaction mixture was warmed to 60°C for 1.5 h. The mixture was then concentrated under reduced pressure, diluted with ice cold water, neutralized with aqueous sodium hydroxide (4N) and extracted with ethyl acetate. The combined organic layers were dried over sodium sulfate, filtered, concentrated and purified via flash chromatography to afford 6-(difluoromethyl)picolinaldehyde (0.2 g, 51%). MS (ES+) (M+H) 158.16; LCMS rétention time: 5.28min (Method H).

Intermediate 38: 3-NÎtro-6-(2-(pvridin-2-vl)morpholino)pyridin-2-amine

To a solution of 2-(pyridin-2-yl) morpholine (0.1 g, 0.609 mmol) in dimethylsulfoxide (5 mL) was added triethylamine (0.17 mL, 1.2 mmol). The resulting solution was stirred for 10 min at room température, and then 6-chloro-3-nitropyridin-2-amine (105 mg, 0.609 mmol) was added. The reaction mixture was heated at 110°C for 4 h, and then was cooled to room température. Ice water was added and the mixture was extracted with ethyl acetate. The combined organic layer was dried over sodium sulfate, filtered and concentrated under reduced pressure. The crude material was purified via flash chromatography (80% ethyl acetate in petroleum ether) to afford the title compound (0.1 g, 55%). MS (ES+) (M+H) 302.25.

Intermediate 39: (ff)-1-(6-Amino-5-nitropvridîn-2-vl)-A/,/V-dimethvlpiperidine-3carboxamide

Step 1: (7?>fert-Butyl 3-(dimethylcarbamoyl)piperidine-1-carboxyiate

To a solution of (7ï>1-(fert-butoxycarbonyl)piperidîne-3-carboxylic acid (10 g, 43.6 mmol) in Ν,Ν-dlmethylformamlde (150 mL) was added 1,1’-carbonyidiîmidazole (14.13 g, 87.23 mmol). The reaction mixture was stirred at room température for 10 min. Triethylamine (18,3 mL, 130.2 mmol) and dimethylamine hydrochloride (7.1 g, 87.2 mmol) were then added sequentiaily. The reaction mixture was stirred at room température for 16 h. The solvent was evaporated and the residue was treated with cold water and was extracted with ethyl acetate. The organic layer was dried over anhydrous sodium sulfate, filtered and concentrated. The residue was purified via flash chromatography to afford (Rf fert-butyl 3-(dimethylcarbamoyl)piperidine-1-carboxyiate (0.9g, 80%). MS (ES+APCI) (M+H) 257.1; LCMS rétention time: 2.829 min (Method D1).

Step 2: (fîJ-/V,/V-Dimethylpiperidine-3-carboxamide hydrochloride

To a solution of (R>tert-butyl 3-(dimethylcarbamoyl)plperidine-1 -carboxyiate (9 g, 35.15 mmol) in methanol (20 mL) at 0°C was added hydrogen chloride in methanol (50 mL). The reaction mixture was stirred at room température for 2 h. The solvent was evaporated in vacuo to afford (7ï)-N₍N-dimethylpiperidine-3-carboxamide hydrochloride (7 g, 90%). MS (ES+APCI) [(M-2HCI)+H] 157.1; LCMS rétention time: 6.36 min (Method F1).

Step 3: (Rfl -(6-Amino-5-nitropyridin-2-yl)-N,N-dimethylpiperidine-3-carboxamÎde

To a solution of (fî>W,W-dimethylpiperidine-3-carboxamide (10 g, 64.04 mmol) in dimethylsulfoxide (100 mL) were added triethylamine (17.8 mL, 128.08 mmol) and 6chloro-3-nitropyridin-2-amine (7.76 g, 44.82 mmol) at room température under a nitrogen atmosphère. The reaction mixture was heated at 110°C for 16 h. The reaction mixture was added to cold water and extracted with ethyl acetate. The organic layer was washed with cold water 3 times, dried over sodium sulfate and evaporated under reduced pressure. The crude compound was purified via flash chromatography and then treated with 1:1 petroleum ether and diethyl ether to afford (fî>1-(6-amino-5nitropyridin-2-yl)-N,N-dimethylpiperidine-3-carboxamide (11 g, 72%). MS (ES+APCl) (M+H) 294.1; LCMS rétention time: 2.777 min (Method E1).

Intermediate 40: ffl)-(1-(6-Amino-5-nitropvridin-2-vl)piperidin-3vD(morpholino)methanone

The title compound was prepared using procedures analogous to the ones used for Intermediate 39, but using morpholine for Step 1, a saturated solution of hydrogen chloride in ether, and diethyl ether as a solvent for Step 2 and acetonitrile for Step 3. . MS (ES+APCl) (M+H) 336.1; LCMS rétention time: 4.033 min (Method I).

Intermediate 41 : Ethyl 4-cvcloDroDvlDicolinimidate

Step 1: 4-Cyclopropylpicolinonitrile

To a solution of 4-bromopicolinonitrile (0.2 g, 1.1 mmol) in tetrahydrofuran were added cyclopropyl boronic acid (0.093 g, 1.1 mmol), and potassium phosphate tribasic (0.694 g, 3.2 mmol). The resulting reaction mixture was degassed with nitrogen for 10 minutes and then added Pd(OAc)₂ and S-phos were added. The reaction mixture was heated at reflux for 16 h,then was diluted with water and extracted with ethyl acetate. The organic layer was concentrated under reduced pressure and the residue was purified via flash chromatography (10% ethyl acetate in petroleum ether) to afford 4cyclopropylpicolinonitrile (0.1 g, 70%). MS (ES+APCl) (M+H) 145.2; LCMS rétention time 3.02 min (Method J).

Step 2: Ethyl 4-cyclopropylpicolinimidate

To a solution of 4-cyclopropyipicolinonitrile (0.04 g, 0.27 mmol) in éthanol was added sodium ethoxide (0.022 g, 0.33 mmol) and the reaction mixture was stirred at room température for 3 h. The reaction mixture was concentrated under vacuum, quenched with water, extracted with ethyl acetate, dried over sodium sulfate, filtered and concentrated under reduced pressure to afford ethyi 4-cyclopropylpicolinimÎdate (0.045

g). The material was used without further purification. MS (ES+APCI) (M+H) 190.9;

LCMS rétention time 1.700 min (Method G).

Intermediate 42: Ethvl 4-cvclopropylDvrimÎdine-2-carbimidate

Step 1: 4-Cyclopropylpyrimidine-2-carbonitrile

To a solution of dîazabicyclooctane (0.013 g, 0.12 mmol) in dimethylsulfoxide and water was added a solution of 2-chloro-4-cyclopropylpyrimidine (0.2 g, 1.2 mmol) in dimethylsulfoxide followed by sodium cyanide (0.066 g, 1.3 mmol). The reaction mixture 10 was stirred at room température for 46 h. The mixture was then poured into water and extracted with ethyi acetate, dried over sodium sulfate and concentrated under reduced pressure. The crude compound was purified via flash chromatography (10% ethyi acetate in petroleum ether) to afford 4-cyc1opropylpyrimidine-2-carbonitrile(0.14 g, 77%). MS (ES+APCI) (M+H) 146.1; LCMS rétention time 3.054 min (Method J).

Step 2: Ethyi 4-cyclopropylpyrimidine-2-carbimidate

Ethyi 4-cyclopropylpyrimidine-2-carbimidate was prepared using a method anaiogous to the one used for Intermediate 41 step 2 but running the reaction for 16h instead of 3h. MS (ES+APCI) (M+H) 192.0.

Intermediate 43: Ethvl 5-(Mmethvlsulfamovl)nicotinimidate

Step 1: 5-Cyano-N-methylpyridine-3-sulfonamide

To a solution of 5-bromo-N-methylpyridine-3-sulfonamide (0.1 g, 0.39 mmol) in Ν,Νdimethylformamide was added zinc cyanide (0.056 g, 0.47 mmol). The solution was degassed with nitrogen for 10 min, then tetrakis(triphenylphosphine)paiiadium(0) (30 25 mg) was added. The reaction mixture was heated to 100°C for 2 h. The mixture was then diluted with water and extracted with ethyi acetate. The organic layer was dried, filtered, and concentrated under reduced pressure. The crude compound was purified by rinsing with diethyl ether to afford 5-cyano-N-methylpyridine-3-sulfonamide (60mg) which was used without further purification.

Step 2: Ethyi 5-(N-methylsulfamoyl)nicotinimidate

To a solution of 5-cyano-/V-methylpyridine-3-sulfonamide (0.1 g, 0.5 mmol) in éthanol was added sodium ethoxide (0.03 g, 0.55 mmol). After 2 h, the mixture was concentrated under reduced pressure. The residue was partitioned between ethyi acetate and water. The organic layer was concentrated under reduced pressure to

W afford ethyl 5-(N-methylsulfamoyl)nlcotinlmidate (120 mg) as an off-white solid. The material was used without further purification. MS (ES+APCI) 244.0; LCMS rétention time: 3.047 min (Method I).

Intermediate 44: 3-(1-Carboxvcvclopropyl)Dvridine 1-oxide

70% meta-Chloroperoxybenzoic acid (1.2 g, 4.9 mmol) was added to a solution of ethyl 1 -(pyridin-3-yl)cyclopropanecarboxylate (400 mg, 2.4 mmol) In dichloromethane (5 mL) at room température and stirred for 16 h. The resulting precipitate was filtered and triturated with dichloromethane to afford the title compound (250mg).

Intermediate 45: 6-(3-(6.7-Dihvdro-5H-pyrrolo[2.1-cïï1,2,41triazol-3-vl)piperidin-1 -vl)-3nÎtropvridin-2-amlne

To a solution of 3-(pîperidin-3-yl)-6,7-dihydro-5H-pyrrolo[2,1-c][1,2,4]triazole (900 mg, 4.68 mmol) in acetonitrile (50 mL) was added 6-chloro-3-nitropyridin-2-amine (730 mg,

4.2 mmol) and triethylamine (1.42 g, 14.04 mmol) at room température under a nitrogen atmosphère. The reaction mixture was stirred for 30 min at reflux, then allowed to cool down to room température and stirred for 16 h. The solvent was removed under reduced ressure and the resulting residue was partitioned between water and ethyl acetate. The organics were dried over sodium sulfate, concentrated under reduced pressure and purified via column chromatography to afford the title compound (1.0 g, 64.9%). MS (ES+) (M+H) 330.33; LCMS rétention time: 2.85 min (Method S).

Intermediate 46:1-(1-Methyl-1 H-pyrazol-5-vlÎcyclopropanecarbonÎtrile

Step 1 : tert-Butyl 2-cyano-2-(1 -methyl-1 H-pyrazol-5-yl)acetate

To a solution of tert-butyl cyanoacetate (340 pL, 2.40 mmol) In 1,4-dioxane (5 mL) was added potassium t-butoxide (1M In tetrahydrofuran, 5 mL, 5.0 mmol) at room température. After 10 min, a solution of 5-iodo-1-methyl-1 H-pyrazole (500 mg, 2.40 mmol) in 1,4-dioxane (5 mL) and tetrakls(triphenylphosphine)palladium(0) (75 mg, 0.065 mmol) were added. The réaction mixture was stirred for 16 h at 70°C. The mixture was cooled and then was diluted with diethyl ether (50 mL) and a 10% aqueous solution of citric acid. The organic layer was separated and the aqueous layer was extracted with diethyl ether (2 x 10 mL). The combined organics were washed with brine (15 mL), dried over magnésium sulfate, filtered, and concentrated under reduced pressure. The ^w residue was purified by silica gel chromatography (Gradient: 30% to 40% ethyl acetate in heptane) to afford tert-butyl 2-cyano-2-(1-methyl-1 H-pyrazol-5-yl)acetate (132.8mg) as a pale brown oil. MS (ES+) (M+H) 222.0, LCMS rétention time: 2.74 min (Method

L); ¹H NMR (500 MHz, CDCI₃) δ 1.50 (s, 9H), 3.93 (s, 3H), 4.80 (s, 1H), 6.39 (d, 1H),

7.48 (d 1 H).

Step 2: 2-(1-Methyl-1 H-pyrazol-5-yl)acetonitrile

A mixture of tert-butyl 2-cyano-2-(1-methyl-1 H-pyrazol-5-yl)acetate (130 mg, 0.588 mmol) in hexafluoroisopropanol (1 mL) was submitted to microwave radiation at 130 °C ίο for 15 minutes. The reaction mixture was diluted with diethyl ether (30 mL), washed with water (5 mL) and brine (5 mL), dried over magnésium sulfate, filtered, concentrated under reduced pressure and dried under high vacuum to afford 2-(1-methyl-1 H-pyrazol5-yl)acetonitrile (68.3 mg) as a pale yeliow oil. ¹H NMR (500 MHz, CDCI₃) δ 3.77 (s, 2H), 3.89 (s, 3H), 6.30 (s, 1H), 7.45 (s, 1H).

Step 3: 1 -(1 -Methyl-1 H-pyrazol-5-yl)cyclopropanecarbonitrile

To a solution of 2-(1-methyl-1 H-pyrazol-5-yl)acetonitrile (65 mg, 0.54 mmol) in Λ/,ΛΛdimethylformamide (3 mL) were added potassium f-butoxide (1M ln tetrahydrofuran, 1.2 mL, 1.2 mmol) and 1,2-dibromoethane (130 pL, 1.5 mmol). The reaction mixture was 2o stirred for 16 h at room température then at 70 °C for 7 h. The mixture was cooled to room température, quenched with water (2 mL) and extracted with diethyl ether (3x10 mL). The combined organic layers were washed with brine (3 mL), dried over magnésium sulfate, filtered, and concentrated In vacuo. The residue was dried under high vacuum. A solution of the residue and 1,2-dibromoethane (65 pL, 0.75 mmol) in 25 Λ/,/V-dimethylfomnamide (0.5 mL) was added dropwise to a suspension of sodium hydride (washed, 23 mg, 1.0 mmol) in Λ/,/V-dimethylformamide (1.0 mL) at room température. After 16 h, additional portions of 1,2-dibromoethane (25 pL, 0.29 mmol) and sodium hydride (8 mg, 0.35 mmol) were added to the reaction mixture at room température. After 2 h, the mixture was quenched with water (3 mL) and extracted with 30 diethyl ether (2x10 mL). The combined organic layers were washed with brine (3 mL), dried over magnésium sulfate, filtered, and concentrated under reduced pressure.

The residue was dried under high vacuum to yield the volatile 1 -(1 -methyl-1 H-pyrazol-5yl)cyclopropanecarbonitrile (20.1 mg, 25%) as a brown oil. ’H NMR (500 MHz, CDCI₃) δ

1.34-1.41 (m, 2H), 1.70-1.76 (m, 2H), 4.03 (s, 3H), 6.10 (d, 1H), 7.39 (d, 1H).

Intermediate 47: Ethvl 1-(m-tolvl)cycloproDanecarbÎmidate hydrochloride

To 1-(m-tolyl)cyclopropanecarbonitrile (100 mg, 0.64 mmol) was added a saturated solution of hydrogen chloride in éthanol (2 mL). After 16 h at room température and 2 h 5 at 70°C and another 16 h at room température, the mixture was concentrated in vacuo and the residue was dried under high vacuum to afford the title compound (147.1 mg, 96.5%) as a coloriess solid. MS (ES+) (M+H) 204.1, LCMS rétention time: 3.10 minutes (Method L).

Intermediate 48: Ethvl 1-(4-methoxvphenvl)cvclopropanecarbimidate hydrochloride

To a cooled solution of 1-(4-methoxyphenyl)cyclopropanecarbonitrile (35 mg, 0.2 mmol) in éthanol (100 pL, 2 mmol) was added acetyl chloride (170 pi, 2.39 mmol). The mixture was shaken at room température for 16 h, then was concentrated in vacuo and dried. To the residue was added a freshly prepared saturated solution of hydrogen chloride in éthanol (1 mL). After 16 h, the reaction mixture was concentrated under reduced pressure to afford the title compound (56 mg) as an oil which was used without further purification.

Intermediate 49: Ethvl 1-i2-methoxyphenvl)cvclopropanecarbimldate hydrochloride

The title compound (50 mg, oil) was prepared by a method analogous to the one used for Intermediate 48 but using 1-(2-methoxyphenyl)cyclopropanecarbonitrile.

Intermediate 50: Ethvl 5-cvclopropvlnlcotinimidate

Step 1: 5-Cyclopropylnicotinonitrile

To a solution of 3-bromo-5-cyanopyridine (1 g, 5.3 mmol) in tetrahydrofuran was added cyclopropyl boronic acid (0.5 g, 5.6 mmol) and potassium phosphate (1.08 g, 15.9 mmol). The resulting solution was degassed with nitrogen for 10 minutes and then palladium (II) acetate and S-phos were added. The reaction mixture was refluxed for 16 h. The reaction mixture was diluted with water, extracted with ethyl acetate and concentrated under reduced pressure. The crude material was purified via column chromatography to afford 5-cyclopropylnicotinonitrile (0.45 g, 58%). MS (ES+) (M+H)

145.1774..

“ Step 2: Ethyl 5-cyclopropylnicotinimïdate

To a solution of 5-cyclopropylnicotinonitrile (0.45g, 3.1 mmol) in éthanol was added sodium ethoxide (0.212 g, 3.1 mmol). The reaction mixture was stirred at room température for 12 h. The solvent was removed under reduced pressure, and the residue was partitioned between water and ethyl acetate. The organic layer was concentrated under reduced pressure to afford the title compound (0.45 g) which was used without further purification. MS (ES+APCI) (M+H) 191.2; LCMS rétention time 1.149 min (Method L1).

îo Intermediate 51 : (fî)-1 -(5,6-Diaminopvridin-2-vl)-N-ethvl-N-methvlpiperidine-3carboxamide

Step 1: (fi)-fert-Butyl 3-(ethyl(methyl)carbamoyl)piperidine-1-carboxylate (fi)-fert-Butyl 3-(ethyl(methyl)carbamoyl)piperidine-1-carboxylate was prepared by a method analogous to the one used for Intermediate 1, Step 1, but using N15 methylethanamlne and N,/V-dimethylformamide. MS (ES+) (M+H) 271.2; LCMS rétention time 2.191 min (Method W).

Step 2: (fi>N-Ethyl-N-methylpiperidine-3-carboxamide To a solution of (fi)-fert-butyl 3-(ethyl(methyl)carbamoyl)piperidine-1-carboxylate (1 g, 20 3.7 mmol) ln diethyl ether (10 mL) at 0°C was added ethereal hydrogen chloride (15 mL). The reaction mixture was warmed to room température and was stirred at that température for 30 min. The solvent was distilled and the resuiting residue was washed with diethyl ether to afford (fi/N-ethyl-N-methylpiperidine-3-carboxamide (1 g). MS (ES+) (M+H) 171.23; LCMS rétention time 2.38 min (Method M1).

Step 3: (fi)-1 -(6-Amino-5-nitropyridin-2-yl)-N-ethyl-N-methylpiperidine-3-carboxamide To a solution of (fi)-N-ethyl-N-methylpiperidine-3-carboxamide (600 mg, 4.1 mmol) in dimethylsulfoxide (6 mL) were added 6-chloro-3-nitropyridin-2-amine (0.58 g, 3.3 mmol) and triethylamine (1.2 mL, 8.3 mmol) at room température. The reaction mixture was 30 heated to 80°C for 18 h. The reaction mixture was poured into ice water, extracted with ethyl acetate and concentrated under reduced pressure. The crude material was purified via column chromatography (10% ethyl acetate in petroleum ether) to afford (fi)-1 -(6-amino-5-nitropyridin-2-yl)-N-ethyl-N-methylpiperidine-3-carboxamide (500 mg,

28.4 %). MS (ES+) (M+H) 308.31.

100

Step 4: (fi)-1 -(5,6-Diaminopyridin-2-yl)-N-ethyl-N-methylpîperidine-3-carboxamide

To a solution of (fi)-1-(6-amino-5-nltropyridin-2-yl)-M-ethyl-M-methylpiperidine-3carboxamide (200 mg, 0.65 mmol) in éthanol (10 mL) was added palladium-on-carbon (0.2 g) in éthanol (10 mL) at room température. The mixture was subjected to a hydrogen atmosphère using a balloon filled with hydrogen gas. The mixture was filtered through Celite to afford the title compound. The filtrate was used without further purification.

Intermediate 52: (fî)-(4-(6-Amino-5-nitropvridin-2-v!)morpholin-2yl)(morpholino)methanone

The title compound was prepared by a method analogous to Intermediate 51, but refluxing the reaction mixture for 30 min and then stirring at room température for 18 h during Step 3 and omitting the final step. MS (ES+APCI) (M+H) 338.2; LCMS rétention time: 2.734 min (Method J).

Intermediate 53: Ethyl 6-ftrif!uoromethyl)picolinlmidate

Sodium ethoxide (197 mg, 2.9 mmol) was added to a solution of 6(trifluoromethy!)pico!lnonitri!e (250 mg, 1.45 mmo!) in ethano! (10 mL) at room température. The reaction mixture was stirred for 2 h. The solvent was removed under reduced pressure and the resulting residue was dissoived in dichloromethane, washed with water, dried over sodium sulfate, and concentrated under reduced pressure to afford the title compound (291 mg) which was used without further purification. MS (ES+) (M+H) 219.16

Intermediate 54: Ethyl 2-(4-ch!oro-1 H-pyrazo!-1-vl)-2-methv!propanimidate

Step 1: 2-(4-Chloro-1H-pyrazol-1-yl)-2-methylpropanenitrile

To a suspension of sodium hydride (630 mg, 16 mmol, 60% dispersion in minerai oil) in dimethylsulfoxide (10 mL) cooled to 18°C was added a solution of 2-(4-ch!oro-1 Hpyrazol-1-yl)acetonitrile (500 mg, 3.53 mmol) and methyliodide (650 pL, 10.0 mmol) In dimethylsulfoxide (5 mL) under nitrogen. The suspension was stirred at room température for 1.5 h. The réaction mixture was added to a cooled solution of saturated aqueous ammonium chloride (25 mL) over a period of 5 min. The mixture was extracted with diethyl ether (20 mL x 3). The combined organics were washed with water (5 mL x 4) and brine (5 mL), dried over magnésium sulfate, filtered and

101 ^w concentrated under reduced pressure to afford 2-(4-chloro-1 H-pyrazol-1 -yl)-2methylpropanenitrile (654.6 mg, >99%) which was used without further puridication. MS (ES+) M+H) 170.0; LCMS rétention time 2.70 (Method L).

Step 2: Ethyl 2-(4-chloro-1 H-pyrazol-1 -yl)-2-methylpropanimidate

To a sodium ethoxide solution, prepared from sodium (20 mg, 1 mmol) and éthanol (4 mL), was added a solution of 2-(4-chloro-1 H-pyrazol-1 -yl)-2-methylpropanenitrile (225 mg, 1.33 mmol) in éthanol (1 mL). The reaction mixture was stirred at 70°C for 30 min. The solvent was removed under a stream of nitrogen and the residue was dried under high vacuum to afford the crude title compound as a colorless solid, which was used io without further purification.

Intermediate 55: Ethyl 1-(4-(methvlthio)-1H-pvrazol-1-vl)cyclopropanecarbimidate hydrochloride

Step 1: 4-(Methylthio)-1H-pyrazole

A suspension of 4-bromopyrazole (4 g, 27 mmol) ln tetrahydrofuran (68 mL) was cooled to 0°C and n-butyllithium (2.5 M in hexanes, 35.9 mL, 90 mmol) was added dropwise over a period of 20 min. The reaction mixture was stirred at room température for 1 h and then was cooled to 0°C. 1,2-Dimethyldisulfide (2.66 mL, 30.0 mmol) was added dropwise. The reaction mixture was stirred at 0°C for 1.5 h. The reaction mixture was poured into water (150 mL) and then it was acidified to pH~8 with a saturated aqueous solution of ammonium chloride and a solution of aqueous hydrochloric acid (1N). The mixture was extracted with ethyl acetate (150 mL x 3) and the combined organic layers were dried over sodium sulfate, filtered and concentrated under reduced pressure to afford 4-(methylthio)-1H-pyrazole (3300 mg). ¹H NMR (500 MHz, CDCI₃) δ 2.36 (s, 3H), 25 7.63 (s, 2H).

Step 2: 2-(4-(MethyIthîo)-1 H-pyrazol-1 -yl)acetonîtrile

Sodium hydride (631 mg, 16 mmol) was added to a solution of 4-(methylthio)-1 Hpyrazole (1500 mg, 13.14 mmol) in anhydrous tetrahydrofuran (30 mL) under nitrogen 30 at 0°C. The réaction mixture was stirred at 0°C until gas évolution was no longer observed. Bromoacetonitrile (1.005 mL, 14.43 mmol) was added dropwise and the reaction mixture was stirred at room température for 18 h. The mixture was poured into a solution of aqueous ammonium chloride (30 mL) and was extracted with ethyl acetate (60 mL x 3). The combined organics were dried over sodium sulfate. The residue was

102 filtered through a plug of silica gel which was eluted with ethyl acetate and the filtrate was concentrated under reduced pressure. The crude matenal was punfied via flash chromatography (0-50% ethyl acetate în heptanes) to afford 2-(4-(methylthio)-1 Hpyrazol-1-yl)acetonitrile (1460 mg, 72.54 %). MS (ES+) (M+H) 154.1; LCMS rétention time 0.40 min (Method N).

Step 3: 1 -(4-(Methylthio)-1 H-pyrazol-1 -yljcyclopropanecarbonitrile

To sodium hydride (2.29 g, 57 mmol, 60% dispersion in minerai oil) was added petroleum ether (40 mL). The suspension was stirred for 10 min and the supematant was removed. Petroleum ether (40 mL) was added agaln and the suspension was stirred for another 10 min. The supematant was removed again after the suspension settled in order to remove the minerai oil. Dimethylsulfoxide (25 mL) was slowly added at 20°C under nitrogen. A solution of 2-(4-(methylthlo)-1H-pyrazol-1-yl)acetonitrile (1.46 g, 9.53 mmol) and 1,2-dibromoethane (2.46 mL, 28.5 mmol) in dimethylsulfoxide (23 mL) was added dropwise over a period of 15 minutes. Additional 1,2-dibromoethane (0.5 mL) was added and the reaction mixture was stirred at room température for 18 h. The reaction mixture was poured into a cold saturated aqueous solution of ammonium chloride (150 mL). The mixture was stirred for 10 min and then it was extracted with diethyl ether (100 mL x 3). The combined organics were washed with brine, dried over sodium sulfate, filtered and concentrated under reduced pressure. The crude material was purified via flash chromatography (95:5 to 60:40 heptane:ethyl acetate) to afford 1(4-(methylthio)-1 H-pyrazol-1 -yljcyclopropanecarbonitrile (390 mg, 22.8%). ¹H NMR (500 MHz, CDCI₃) δ 1.79-1.83 (m, 4H), 2.36 (s, 3H), 7.52 (s, 1H), 7.58-7.60 (m, 1H).

Step 4: Ethyl 1-(4-(methylthio)-1 W-pyrazol-1-yl)cyclopropanecarbimidate hydrochloride To a saturated solution of hydrogen chloride in éthanol (3.5 mL) at 0°C was added a solution of 1-(4-(methylthio)-1 W-pyrazol-1 -yljcyclopropanecarbonitrile (125 mg, 0.697 mmol) in éthanol (0.5 mL). The reaction mixture was stirred at room température for 1.5h. The solvent was removed under a stream of nitrogen to afford the title compound (210 mg). The material was used without further purification.

Intermediate 56: Ethyl 1-(4-chloro-1H-Dvrazol-1-vl)cvcloDroDanecarbimidate

Step 1: 1-(4-Chloro-1H-pyrazol-1-yljcyclopropanecarbonitrile

103

V Into a 4-neck 2 L round bottom flask, previously dried with a heat gun under high vacuum, was added dimethylsulfoxide (175 mL). The flask was placed in a 10°C bath and sodium hydride (60% oil dispersion, 17.0 g, 300 mmol) was added portionwise with stirring under nitrogen. The resulting suspension was stirred for 10 min before a solution of 2-(4-chloro-1 H-pyrazol-1 -yljacetonitrile (10.0 g, 70.6 mmol) and 1,2dibromoethane (18.3 mL, 213 mmol) in dimethylsulfoxide (175 mL) was added dropwise over a period of 30 min. The internai température was kept between 13 and 20°C during the addition process. The reaction mixture was stirred for 5.5 h while keeping the internai température at or below 20°C, followed by stirring for an additional 1 h at room température. The mixture was cooled to 10°C, and then a saturated aqueous solution of ammonium chloride (600 mL) was slowly added. The mixture was stirred at 10°C for 15 min, then extracted with ethyl acetate (1000 mL x 3). The combined organics were washed with brine, dried over sodium sulfate, filtered and concentrated under reduced pressure. The crude material was purified via flash chromatography (1015 100% dichloromethane in hexanes), and the residue was crystallized from a mixture of hexanes/diethyl ether to afford 1-(4-chloro-1 H-pyrazol-1 -yljcyclopropanecarbonitrile (7.654 g). ’H NMR (400 MHz, CDCI₃) δ 1.80-1.82 (m, 4H) 7.47 (d, 1 H) 7.62 (d, 1 H); MS (EI+) (M+) 167; GCMS rétention time 1.64 minutes (Method O).

Step 2: Ethyl 1-(4-chloro-1 H-pyrazol-1 -yl)cyclopropanecarbimidate

To a solution of 1 -(4-chloro-1 H-pyrazol-1 -yl)cyclopropanecarbonitrile (17.3 g, 103 mmol) in éthanol (50 mL) was added sodium ethoxide (prepared from 3.2 g, 139 mmol of sodium métal dissolved in 150 mL of éthanol). The reaction mixture was stirred at 70°C for 2 h to afford ethyl 1-(4-chloro-1 H-pyrazol-1-yljcyclopropanecarbimidate which was used for the next step without further purification. An aliquot of the solution was concentrated for analysis: ’H NMR (400 MHz, CDCI₃): δ □ 1.25 (t, 3H), 1.46 (q, 2H), 1.73 (q, 2H), 4.15 (q, 2H), 7.51 (s, 1H), 7.52 (s, 1H).

Intermediate 57: Ethyl 1-(4-methoxv-1H-Dvrazol-1-vl)cvclooropanecarbimidate

The titie compound was prepared by a method analogous to the one used for

Intermediate 56, but using 2-(4-methoxy-1 H-pyrazol-1-yl)acetonitrile as the starting material. ’H NMR (500 MHz, CDCI₃) δ 1.37 (t, 3H), 1.63-1.72 (m, 4H), 3.76 (s, 3H), 4.16 (q, 2H), 7.17 (s, 1 H), 7.35 (s, 1 H).

104

Intermediate 58: 1-(2-Cvclopropvloxazol-4-vOcvcloorooanecarboxylÎc acid

Step 1 : Ethyl 2-(2-cyclopropyloxazol-4-yl)acetate

To a solution of cyclopropanecarboxamide (3.5 g, 41.46 mmol) in toluene and 1,4dioxane (50 mL, 1:1) was added ethyl 4-chloro-3-oxobutanoate (20.0 g, 121.95 mmol).

The mixture was heated to 100°C for 18 h, then was diluted with water and extracted with ethyl acetate. The organic layer was dried and concentrated under reduced pressure. The crude material was purified via column chromatography on 100-200 mesh silica (20% ethyl acetate in petroleum ether) to afford ethyl 2-(2cyc!opropyloxazo!-4-yl)acetate (3.5 g). MS (ES+APCI) 196.1; LCMS rétention time:

4.029 min (Method Q1).

Step 2: Ethyl 1-(2-cyclopropyloxazol-4-yl)cyclopropanecarboxylate Césium carbonate (8.39 g, 25.78 mmol) 1,2-dibromoethane were added to a solution of ethyl 2-(2-cyclopropyloxazol-4-yl)acetate (2.0 g, 10.03 mmol) in Λ/,Ν-dimethylformamide (10 mL) at room température. The reaction mixture was stirred at room température for

h. The mixture was diluted with water, then was extracted with ethyl acetate, dried and concentrated under reduced pressure. The crude material was purified via column chromatography on 100-200 mesh silica (10% ethyl acetate in petroleum ether) to afford ethyl 1-(2-cyclopropyloxazol-4-yl)cyclopropanecarboxylate (200 mg). MS (ES+) (M+H)

222.2; LCMS rétention time: 2.514 min (Method N1).

Step 3: 1-(2-Cyclopropyloxazol-4-yl)cyclopropanecarboxylic acid

An aqueous solution of sodium hydroxide (4N, 5 mL) was added to a solution of ethyl 1(2-cyclopropyloxazol-4-yl)cyclopropanecarboxylate (200 mg, 0.9 mmol) in tetrahydrofuran and water (4 mL, 1:1 ). The reaction mixture was stirred at room température for 12 h. The mixture was acidified with an aqueous solution of hydrochioric acid (4N), extracted with ethyl acetate, dried and concentrated under reduced pressure to afford the title compound (150 mg). MS (ES+APCI) (M+H) 194.1 ; LCMS rétention time: 3.515 min (Method Q1).

Intermediate 59: Ethyl 2-(4-fluoro-1H-pvrazoM-v0propanimldate

Step 1: 2-(4-Fluoro-1H-pyrazol-1-yl)propanenitrile

4-Fluoro-1 H-pyrazole (300 mg, 3.49 mmol), 2-chloropropanenitrile (374 mg, 4.18 mmol), césium carbonate (1,84 g, 5.23 mmol) and anhydrous acetonitrile (5.0 mL) were added

105 ^w Into a round bottom flask. The reaction mixture was heated to 100°C for 2 h. Water was added and the mixture was extracted with ether (100 mL x 3). The combined organic layers were dried over sodium sulfate, filtered and concentrated under reduced pressure. The crude material was purified via flash chromatography (0-50% ethyl acetate in heptanes) to afford 2-(4-Fluoro-1 H-pyrazol-1 -yljpropanenitrile (398 mg, 82%). MS (EI+) (M+) 139, GCMS rétention time: 0.78 min (Method O).

Step 2: Ethyi 2-(4-fluoro-1 H-pyrazol-1 -yl)propanimidate

To a solution of sodium ethoxide, prepared by adding solid sodium métal (64.4 mg, 2.80 mmol) to éthanol (10 mL), was added a solution of 2-(4-fluoro-1H-pyrazol-110 yljpropanenitrile (390 mg, 2.80 mmol) in éthanol (2 mL). The reaction mixture was stirred at 70°C for 18 h. The mixture was cooled to room température and used without further purification. MS (EI+) (M+) 186; GCMS rétention time: 1.27 min (Method O).

Intermediate 60: Ethyl 2-(4-chloro-1H-pvrazol-1-vl)propanlmidate

Step 1: 2-(4-Chloro-1 H-pyrazol-1 -yljpropanenitrile

4-Chloro-1 H-pyrazole (4.05 g, 39.5 mmol), 2-chloropropanenitrile (3.71 g, 41.5 mmol), césium carbonate (15.6 g, 44.3 mmol) and anhydrous tetrahydrofuran (20 mL) were added into a sealed tube and the reaction mixture was heated to 100°C for 2 h. The mixture was diluted with ether (100 mL) and filtered. The solids were rinsed with ether (30 mL x 3) and the filtrate was diluted with dichloromethane (10 mL). The solution was concentrated under reduced pressure to afford the title compound as oil (7.15 g). The material was taken to the next step without further purification. ¹H NMR (500 MHz, CDCh) δ 1.90 (d, 3H), 5.21-5.30 (m, 1 H), 7.51 (s, 1 H), 7.58 (s, 1H); MS (EI+) (M+) 155; GCMS rétention time: 1.23 minutes (Method O).

Step 2: Ethyl 2-(4-chloro-1 H-pyrazol-1-yl)propanimidate

A solution of sodium ethoxide, prepared by adding soiid sodium (420 mg, 18.2 mmol) into éthanol (20 mL), was added to a solution of 2-(4-chloro-1 H-pyrazol-1 yljpropanenitrile (2530 mg, 13.0 mmoi) in éthanol (4 mL). The reaction mixture was stirred at 70°C for 3 h. ’H NMR analysis of the reaction mixture showed desired product. The mixture was used without further purification. ’H NMR (500 MHz, CDCI₃) δ 1.30 (t, 3H), 1.75 (m, 3H), 4.19 (m, 2H), 4.86 (m, 1H), 7.47 (s, 1 H), 7.52 (s, 1H).

106

Intermediate 61: 1 -(Pvrazin-2-vDcvcloproDanecarboxvlic acid

Step 1: 1-(Pyrazin-2-yl)cyclopropanecarbonitrile

To a suspension of sodium hydride (0.386 g, 16.1 mmol) in anhydrous N,Ndimethylformamide (5 mL) was added dropwise a solution of 2-(pyrazin-2-yl)acetonitrile (0.55 g, 4.6 mmol) and 1,2-dibromoethane (2.25 g, 11.9 mmoi) in anhydrous N,Ndimethylformamide (8 mL) over a period of 10 min at room température. The mixture was stirred for 18 h, then was quenched with water and extracted with ethyi acetate. The organic layer was washed with brine, dried over sodium sulfate, filtered, and concentrated under reduced pressure. The crude material was purified via column chromatography (100-200 mesh silica gel, 100% dichloromethane) to afford 1-(pyrazin-

2-yl)cyclopropanecarbonitrile (210mg, 31%).

Step 2: 1-(Pyrazin-2-yl)cyclopropanecarboxylic acid

A 20% aqueous sodium hydroxide solution (1 mL) was added to a solution of 1 15 (pyrazln-2-yl)cyclopropanecarbonitrile (0.2 g, 1.37 mmol) in methanol (10 mL) at room température. The reaction mixture was heated at reflux for 36 h. The mixture was cooled, and then the solvent was removed under reduced pressure. The resulting residue was partitioned between water and ethyi acetate. The aqueous layer was neutralized with aqueous hydrochloric acid (3N) and the water was removed under reduced pressure. To the residue was added methanol and dichloromethane. The mixture was filtered to remove the insoluble solids and the filtrate was dried over sodium sulfate, filtered and concentrated under reduced pressure to afford the title compound (100 mg). MS (ES-) (M-1) 162.9.

Intermediate 62: 1-(Pvrimidin-2-vl)cvcloproDanecarbaldehvde

Step 1: 1-(Pyrimldîn-2-yl)cyc!opropanecarbonitrile 1-(Pyrimidin-2-yl)cyclopropanecarbonitrile was prepared by a method anaiogous to the one used for Intermediate 61, Step 1. MS (ES+) (M+H) 146.1.

Step 2: 1-(Pyrimldin-2-yl)cyclopropanecarbaldehyde

Diisobutylaluminum hydride (1M in tetrahydrofuran, 10.9 mL, 11 mmol) was added dropwise to a solution of 1-(pyrimidin-2-yl)cyclopropanecarbonitrile (0.2 g, 1.37 mmol) in anhydrous toluene (10 mL) at -78°C over a period of 15 min. The reaction mixture was warmed to room température and was stirred at room température for 30 min. The

107 mixture was then cooled back to -78°C and another portion of diisobutylaluminum hydride (1M in tetrahydrofuran, 10.9 mL, 11 mmol) was added. The reaction mixture was warmed to room température and stirred for another 30 min. The mixture was quenched with aqueous sodium hydroxide (2N) at -20°C and then stirred at room température for 30 min. The mixture was filtered through a pad of Celite and the filtrate was extracted with ethyl acetate. The organics were dried over sodium sulfate, filtered and concentrated under reduced pressure to afford the title compound (0.125 g). The material was used without further purification. MS (ES+APCI) (M+H) 149.0.

Intermediate 63: Ethyl l-dH-pyrazoM-vhcvclopropanecarbimidate

Step 1: 1 -(1 H-Pyrazol-1 -yl)cyclopropanecarbonitrile

Sodium hydride (4.65 g, 121 mmol, 60% oil dispersion) was washed with petroleum ether under a nitrogen atmosphère. The supematant was removed after the suspension settled in order to remove the minerai oil. Then a solution of 2-(1 H-pyrazol15 1 -yl)acetonitrile (3 g, 27 mmol) and 1,2-dibromoethane (6.98 mL, 81 mmol) in dimethylsulfoxide (90 mL) was added at 20 °C over a period of 40 min. The reaction mixture was stirred at room température for 5 d. A saturated aqueous solution of ammonium chloride was added and the mixture was stirred at 0 °C for 15 min, extracted with diethyl ether, washed with brine, dried over sodium sulfate and concentrated under reduced pressure. The resulting crude was purified via column chromatography (100200 mesh silica gel, 5-12% ethyl acetate in petroleum ether) to afford 1-(1H-pyrazol-1yljcyclopropanecarbonitrile (1 g, 28%).

Step 2: Ethyl 1-(1H-pyrazol-1-yl)cyclopropanecarbimidate

Sodium métal (12 mg, 0.52 mmol) was added to anhydrous éthanol (3 mL) at room température and the mixture was stirred for 15 min. 1-(1 H-pyrazol-1yl)cyclopropanecarbonitrile (0.1 g, 0.75 mmol) dissolved in éthanol (2 mL) was added and the reaction mixture was heated to 70 °C for 90 min. The resulting solution was used without further purification.

Intermediate 64: Ethyl 1-i4-fluoro-1H-pvrazol-1-vl)cvclopropanecarbimidate

Step 1: 2-(4-Fluoro-1 H-pyrazol-1 -yl)acetonitrile

Sodium hydride (306 mg, 12.78 mmol, 60% oil dispersion) was suspended in tetrahydrofuran and the suspension was cooled to 0°C. A solution of 4-fluoro-1 H108 pyrazole (1.0 g, 11.62 mmol) and 2-bromoacetonitrile (1.39 g, 11.62 mmol) in tetrahydrofuran at 0°C was added dropwise over a period of 40 min. The réaction mixture was stirred at room température for 12 h, and then a saturated aqueous solution of ammonium chloride was added. The mixture was extracted with diethyl ether, and the organic layer was washed with brine, dried over sodium sulfate, filtered and concentrated under reduced pressure. The crude material was purified via column chromatography (100-200 mesh silica gel, 5-10% ethyi acetate in petroleum ether) to afford 2-(4-f!uoro-1H-pyrazo!-1-y!)acetonitri!e (1 g).

Step 2: 1 -(4-Fiuoro-1 H-pyrazol-1 -yljcyclopropanecarbonïtrile

Sodium hydride (1.26 g, 52.75 mmol, 60% oil dispersion) was washed with petroleum ether under a nitrogen atmosphère. The supematant was removed after the solids settled. Then a solution of 2-(4-fluoro-1H-pyrazoi-1-yl)acetonitrile (1.1 g, 8.79 mmol) and 1,2-dibromoethane (2.28 mL, 26.37 mmol) in dimethylsulfoxide (90 mL) was added at 0°C over a period of 40 min. The reaction mixture was stirred at room température for 6 h. A saturated aqueous solution of ammonium chloride was added and the mixture was extracted with diethyl ether. The organic layer was washed with brine, dried over sodium sulfate, filtered and concentrated under reduced pressure. The crude material was purified via column chromatography (100-200 mesh silica gel, 5-12% ethyi acetate in petroleum ether) to afford 1-(4-fluoro-1H-pyrazol-1y!)cyclopropanecarbonitrile (300 mg).

Step 3: Ethyi 1-(4-fluoro-1H-pyrazol-1-yl)cyc!opropanecarbimldate

Sodium métal (47 mg, 1.98 mmol) was added to anhydrous éthanol (4 mL) at room température. The mixture was stirred for 15 min. A solution of 1-(4-fluoro-1H-pyrazol-1yl)cyclopropanecarbonitrile (0.1 g, 0.66 mmol) in éthanol (2 mL) was added and the reaction mixture was heated to 70°C for 90 min. The resulting solution was used without further purification.

Intermediate 65: Ethvl 1-(2H-1,2,3-triazol-2-vl)cvcloDroDanecarbimldaÎe The title compound was prepared by a method anaiogous to the one used for Intermediate 64, but using 1,2,3-triazole as the starting material.

109

Φ Intermediate 66: 1-(1H-lmidazol-1-vl)cvclopropanecarbaldehvde

Step 1: Ethyl 1-(1 H-imidazol-1-yl)cyclopropanecarboxylate Ethyl 1-aminocyclopropanecarboxylate hydrochloride (850 mg, 6.58 mmol), phosphoric acid (85%, 0.2 mL), glyoxal (40%, 0.76 mL) and formaldéhyde (37%, 0.5 mL) In water (4 mL) were stirred for 15 min at room température. The reaction mixture was then heated to 90°C followed by the addition of ammonium chloride (354 mg) In water (3 mL). The resulting mixture was stirred at 90°C for 1 h. The mixture was cooled to room température and was neutralized using aqueous potassium hydroxide (3N) at 10°C. The solvent was removed under reduced pressure and co-distilled with toluene. The crude material was purified via column chromatography (100-200 mesh silica gel, 0-5% methanol In dichloromethane) to afford ethyl 1-(1H-lmidazol-1yl)cyclopropanecarboxylate (350 mg).

Step 2: (1-(1H-lmidazol-1-yl)cyclopropyl)methanol

Diisobutylaluminum hydride (1M in tetrahydrofuran, 8 mL, 8 mmol) was added dropwise to a solution of ethyl 1 -(1 H-imidazol-1 -yl)cyclopropanecarboxylate (0.3 g, 1.6 mmol) In anhydrous dichloromethane (20 mL) at -78°C. The reaction mixture was warmed to room température and stirred at that température for 5 h. A saturated aqueous solution of ammonium chloride was added and the mixture was stirred for 30 min. The resulting solids were fiitered through a pad of Celite and the filtrate was dried over sodium sulfate. The organics were concentrated under reduced pressure and the crude material was purified via column chromatography (100-200 mesh silica gel, 0-6% methanol in dichlormethane) to afford (1-(1 H-imidazol-1 -yl)cyclopropyl)methanol 0.15 g). MS (ES+APCI) (M+H) 139.1.

Step 3: 1-(1H-lmidazol-1-yl)cyclopropanecarbaldehyde

To a solution of (1-(1 H-imidazol-1 -yl)cyclopropyl)methanol (42.39 mg, 0.307 mmol) in anhydrous ethyl acetate (10 mL) was added 2-lodoxybenzoic acid (IBX) (129 mg, 0.461 mmol). The reaction mixture was heated at reflux for 2 h. The mixture was fiitered and the filtrate was concentrated to afford the title compound which was used for the next step without further purification.

Intermediate 67: Ethyl 1-(1-methyl-1 H-1,2.4-Îriazol-5-vl)cvclopropanecarbimidate

Step 1: 5-(Chloromethyl)-1-methyl-1 H-1,2,4-triazole hydrochloride

110

Thionyl chloride (20 mL) was added to (1 -methyl-1 H-1 ^^-triazol-S-ylJmethanol (2.0 g) at 0°C. The réaction mixture was stirred for 2 h at 80°C. The mixture was cooled to room température and then was concentrated under reduced pressure. Ethyl acetate was added to the resulting precipitate and the mixture was filtered under a nitrogen atmosphère to afford 5-(chloromethyl)-1 -methyl-1 H-1,2,4-triazole hydrochloride (1.5 g). The material was used for the next step without further purification. MS (EI+) (M+) 131.0

Step 2: 2-(1 -Methyl-1 H-1 ^^-triazol-S-ylJacetonitrile

A solution of 5-(chloromethyl)-1 -methyl-1 H-1,2,4-triazole hydrochloride (1.5 g, 11.45 mmol) in dimethylsulfoxide (30 mL) was added to a stirred suspension of sodium cyanide (3.366 g, 68.70 mmol) in dimethylsulfoxide (20 mL), and the reaction mixture was stirred at room température for 16 h. The mixture was partitioned between ethyl acetate and water. The organic layer was dried over sodium sulfate and concentrated.

The resulting crude product was purified via column chromatography (100-200 mesh silica gel, 10-12% ethyl acetate in petroleum ether) to afford 2-(1 -methyl-1 H-1,2,4triazol-5-yl)acetonitrile (1.2 g). MS (ES+APCI) (M+H) 123.2.

Step 3: 1 -(1 -Methyl-1 H-1,2,4-triazol-5-yl)cyclopropanecarbonitrile

Sodium hydride (60% dispersion in minerai oil, 1.06 g, 44.2 mmol) was washed twice with petroleum ether under nitrogen. Then dimethylsulfoxide (10 mL) was added at room température. A solution of 2-(1 -methyl-1 H-1 ^-triazol-S-yOacetonitrile (0.9 g, 7.36 mmol) and 1,2-dibromoethane (4.147 g, 22.1 mmol) in dimethylsulfoxide (20 mL) was added dropwise to the sodium hydride suspension over a period of 20 min. The réaction mixture was stirred at room température for 3 h. The mixture was partitioned between ethyl acetate and water. The organic layer was dried over sodium sulfate and concentrated under reduced pressure. The crude material was purified via column chromatography (100-200 mesh silica gel, 5-15% ethyl acetate in petroleum ether) to afford 1-(1-methyl-1 H-1,2,4-triazol-5-yl)cyclopropanecarbonitrile (0.7 g) as a coloriess oil.

Step 4: Ethyl 1 -(1 -methyi-1 H-1,2,4-triazol-5-yl)cyclopropanecarbimidate

Sodium métal (37 mg, 1.61 mmol) was added to anhydrous éthanol (5 mL) at room température. The mixture was stirred for 30 min. A solution of 1-(1 -methyl-1 H-1,2,4111

V triazol-5-yl)cyclopropanecarbonîtrile (0.3 g, 2.0 mmol) in éthanol (5 mL) was added and the reaction mixture was heated to 60’C for 1 h. The resulting mixture was used without further purification. MS (ES+APCI) (M+H) 195.1.

Intermediate 68: (Fn-2-(4-Chloro-1 H-pyrazol-1-yl)propanoic acid s Step 1 : (S)-Methyl 2-(trifluoromethylsulfonyloxy)propanoate

2,6-Lutidine (1.2 mL, 10 mmol) and methyl (S/lactate (1.041 g, 10.0 mmol) were dissolved in a mixture of heptanes:dichloromethane (4:1,10 mL) under nitrogen. The solution was cooled to -10°C using an ice-brine bath. Trifluoromethanesulfonic anhydride (2.0 mL, 12 mmol) was added dropwise over 10 min while stirring the cooled 10 solution. After the addition was completed, the reaction mixture was stirred at -10°C for min, then quenched with aqueous hydrochloric acid (0.5 M, 15 mL) and stirred at 10°C for another 30 min. The resulting mixture was transferred to a separatory funnel and the phases were separated. Silica gel (2g) was added to the funnel containing the organic layer and the mixture was shaken, and then filtered through a 2 g silica gel plug 15 (eluting with 40 mL of a 3% ethyl acetate in heptanes solution). The filtrate was concentrated at room température to afford (S)-methyl 2(trifluoromethylsulfonyloxy)propanoate as a clear oil (1.538g), ¹H NMR (500 MHz, CDCI₃) δ 1.72 (d, 3H), 3.87 (s, 3H), 5.24 (q, 1H).

Step 2: (R)-Methyl 2-(4-chloro-1 H-pyrazol-1-yl)propanoate

4-Chloro-1 H-pyrazole (668 mg, 6.51 mmol) was dissolved in ethyl acetate and (S)methyl 2-(trifluoromethylsulfonyloxy)propanoate (1.538 g, 6.51 mmol) was added followed by potassium carbonate (2.70 g, 19.5 mmol) while stirring at room température. The reaction mixture was stirred for 18 h. The mixture was diluted with methyl fert-butyl 25 ether (15 mL), filtered through Celite, and washed with a mixture of ethyl acetate:methyl fert-butyl ether (1:1,15 mL x 4). The filtrate was reduced in volume by approximately 30-40 mL then was washed with aqueous hydrochloric acid (0.1 M, 25 mL), water and brine (15 mL). The organic phase was dried over magnésium sulfate, filtered and concentrated to afford (R)-methyl 2-(4-chloro-1 H-pyrazol-1-yl)propanoate as an oil (1.02 30 g). ’H NMR (500 MHz, CDCI₃) δ 1.77 (d, 3H), 3.76 (s, 3H), 5.05 (q, 1 H), 7.46 (s, 1 H),

7.54 (s, 1H); MS (EI+)(M+) 188; Chiral HPLC rétention time: 3.11 min (Method:

Chiralpak AD-H 0.46x25 cm, Mobile Phase: 95/5 CO₂/methanol; Flow: 2.5 mL/min);

73.3% ee.

112

Step 3: (fi)-2-(4-Chloro-1 H-pyrazol-1 -yl)propanolc acid (fi)-Methyl 2-(4-chloro-1 H-pyrazol-1-yl)propanoate (1.00 g, 5.30 mmol) was suspended in a solution of aqueous hydrochloric acid (6M, 8.Θ mL, 53 mmol). The reaction mixture s was stirred for 18 h at reflux. The mixture was cooled to 0°C and quenched with a saturated aqueous solution of sodium phosphate (NaHaPO^). The pH was adjusted to

-2 using aqueous sodium hydroxide and hydrochloric acid. The mixture was extracted with ethyl acetate (20 mL x 3). The combined organics were washed with brine, dried over magnésium sulfate, filtered and concentrated. The residue was dried under îo vacuum, dissolved in a hot solution of heptanes:ethyl acetate (3:1,9 mL) and allowed to cool to room température. A seed crystal was added while cooling. The mixture was stirred for 18 h. The solids were collected by filtration and washed with a solution of heptanes:ethyl acetate (3:1, 2 mL x 2) and heptanes (2 mL x 2). The resulting solids were air dried to afford (fi)-2-(4-chloro-1 H-pyrazol-1-yl)propanoic acid (310 mg, 33%).

¹H NMR (500 MHz, CDCI₃) δ 1.8 (d, 3H), 5.13 (q, 1 H), 7.51 (s, 1H). 7.55 (s, 1H), 11.8 (s, 1H); MS (EI+)(M+) 174; Chiral HPLC rétention time: 3.81 min (Method: Chiralpak AD-H 0.46x25 cm, Mobile Phase: 90/10 COi/methanol; Flow: 2.5 mL/mîn); >99.5% ee.

Intermediate 69: 1-(Pvridazin-3-vDcvcloDroDanecarboxvlÎc acid

Step 1 : Ethyl 2-(pyridazin-3-yl)acetate

Lithium diisopropylamide (2M in tetrahydrofuran, 6.83 g, 63.7 mmol) was added dropwise to a stirred solution of 3-methylpyridazine (5.0 g, 53.12 mmol) in anhydrous tetrahydrofuran (80 mL) at -78°C under a nitrogen atmosphère over a period of 20 min. The mixture was allowed to warm to room température. The mixture was stirred for 1 h at room température and cooled again to -78°C. Diethyl carbonate (12.57 g, 106 .2 mmol) in anhydrous tetrahydrofuran (20 mL) was added at -78°C over a period of 20 min. The reaction mixture was allowed to warm to room température, stirred for 16 h and partitioned between ethyl acetate and a saturated aqueous solution of ammonium chioride. The organics were dried over sodium sulfate and concentrated under reduced pressure. The resulting crude material was purified via colulmn chromatography (10030 200 mesh silica gel, 50-80% ethyl acetate ln petroleum ether) to afford ethyl 2(pyridazin-3-yl)acetate (600 mg). MS (ES+) (M+H) 167.1569.

113 • Step 2: Ethyl 1-(pyridazin-3-yl)cyclopropanecarboxylate

Sodium hydride (60% suspension ln minerai oil, 830 mg, 21.66 mmol) was washed with petroleum ether under a nitrogen atmosphère to remove the minerai oil.

Dimethylsulfoxide (10 mL) was added and the resulting suspension was stirred for 5 min before adding ethyl 2-(pyridazin-3-yl)acetate (600 mg, 3.61 mmol) and 1,2dibromoethane (2.03 g, 10.83 mmol) dissolved in dimethylsulfoxide (10 mL) at 10°C. The reaction mixture was allowed to warm to room température and stirred for 2 h. The mixture was partitioned between a saturated aqueous solution of ammonium chloride and ethyl acetate. The organics were dried over sodium sulfate and concentrated under reduced pressure. The resulting crude material was purified via column chromatography (100-200 silica gel mesh, 50-60% ethyl acetate in petroleum ether) to afford ethyl 1-(pyridazin-3-yl)cyclopropanecarboxylate (300 mg). MS (ES+APCI) (M+H) 193.0.

Step 3: 1-(Pyridazin-3-yl)cyclopropanecarboxylic acid

Lithium hydroxide (196.4 mg, 4.68 mmol) was added to a solution of ethyl 1-(pyridazin-

3-yl)cyclopropanecarboxylate (300 mg, 156 mmol) in tetrahydrofuran and water (1:1,10 mL) at room température. The réaction mixture was stirred for 2 h and concentrated. The resulting residue was dissolved in water and the pH was adjusted to 2 using 1N aqueous hydrochloric acid. The mixture was extracted with ethyl acetate. The organics were dried over sodium sulfate and concentrated to afford the title compound (160 mg) which was used as Is without further purification. MS (ES+APCI) (M-H) 163.0; LCMS rétention time: 2.713 minutes (Method X1)

Intermediate 70: 1 -(4-Chloro-1 H-pyrazol-1 -vDcvclopropanecarboxylic acid

Step 1: tert-Butyl 1-(4-chloro-1 H-pyrazol-1-yl)cyclopropanecarboxylate

Into a flask was added potassium trimethylsilanolate (20.85 g, 146.31 mmol), 4-chloro1 H-pyrazole (15 g, 146.31 mmol) and 2-methyltetrahydrofuran (400 mL). The mixture was stirred for 5 min followed by the addition of tert-butyl 2,4-dibromobutyrate (27.84 mL, 146.31 mmol) over a period of 20 to 30 seconds. The reaction mixture was stirred for 60 min. Potassium trimethylsilanolate (20.85 g, 146.31 mmol) in 230 methyltetrahydrofuran (80 mL) was added over a period of 5 min. The reaction mixture was stirred for 1 h before adding tert-butyl 2,4-dibromobutyrate (1 mL, 5.3 mmol). After 30 min, an additional portion of tert-butyl 2,4-dibromobutyrate (2 mL, 10.5 mmol) was added. The mixture was stirred for 40 min before adding tert-butyl 2,4-dibromobutyrate

114 “ (1 mL, 5.3 mmol) and potassium trimethylsilanolate (2 g, 14 mmol). The reaction mixture was stirred for 18 h. Hydrochloric acid (146.31 mL, 146.31 mmol) was added and the mixture was stirred for 5 min. The layers were separated, and the organics were washed with water (200 mL) and concentrated under reduced pressure. Toluene (100 mL) was added and the mixture was concentrated to dryness to afford tert-butyl 1 (4-chloro-1H-pyrazol-1-yl)cyclopropanecarboxylate (45 g). ’H NMR (400 MHz, CDCI₃): δ □ 1.40 (s, 9H), 1.57 (q, 2H), 1.75 (q, 2H), 7.44 (s, 1H), 7.51 (s, 1 H); MS (EI+) (M+) 242; GCMS rétention time: 2.37 minutes (Method O).

Step 2: 1 -(4-Ch!oro-1 H-pyrazol-1 -yl)cyclopropanecarboxyfic acid

To a solution of tert-butyl 1 -(4-chloro-1 H-pyrazol-1 -yl)cyc!opropanecarboxylate (45 g) in toluene (60 mL) was added trifluoroacetic acid (33.19 mL, 438.92 mmol). The reaction mixture was heated to 45°C for 3 h, then cooled to room température and stirred for 18 h. A further portion of trifluoroacetic acid (2 mL) was added. The reaction mixture was heated to 45°C and stirred for 1 h. The mixture was reduced in volume under vacuum in a jacketed vessel with the température set at 50°C. Toluene (200 mL) was added and the mixture was concentrated to low volume, and then this was repeated with a further 200-mL portion of toluene. Toluene (200 mL) was added, and the mixture was stirred at room température for 18 h. The organics were washed sequentially with a 2N aqueous solution of potassium hydroxide (150 mL), and 1N aqueous solution of potassium hydroxide (150 mL). The aqueous layers were combined and acidified to pH-1 with concentrated hydrochloric acid, then extracted with ethyl acetate (200 mL x 2). The combined extracts were concentrated to low volume and the residue was diluted with ethyl acetate (100 mL) and concentrated to dryness to afford an orange solid (32 g). Toluene (136 mL) was added to the solid and the mixture was heated to

60°C and stirred for 15 min until ail the solids dissolved, then cooled to 40°C, held at that température for 1 h, and then cooled to 36°C and held at that température for 1 h. The mixture was warmed to 50°C and n-heptane (136 mL) was added dropwise over a period of 30 min. The mixture was kept at 50°C for 10 min until the solution became turbid. The température was increased to 55°C, held for 1.5 h, cooled to room température over a period of 2 h and granulated over a period of 18 h. The mixture was filtered and the solids were washed with a mixture of toluene:heptanes (1:1,55 mL) and dried in a vacuum oven at 40°C for 4 h to afford the title compound as a white solid (11.40 g, 41.76%). ’H NMR (400 MHz, DMSO-cfe) δ 1.41-1.71 (m, 4H), 7.54 (s, 1H),

115

8.12 (s, 1H), 13.09 (s, 1H); MS (EI+) (M+) 186; GCMS rétention time: 2.56 minutes (Method O).

Alternative préparation for 1-(4-chloro-1H-pyrazol-1-yl)cyclopropanecarboxylic acid Step 1: tert-Butyl 1-(4-chloro-1H-pyrazol-1-yl)cyclopropanecarboxylate

4-Chloro-1 H-pyrazole (2.87 kg, 28.0 mol) and tert-butyl 2,4-dibromobutyrate (9.89 kg, 32.8 mol) were dissolved in 2-methyltetrahydrofuran (26.0 L) and the resulting solution was cooled to 5°C. A suspension of potassium trimethylsilanolate (9.58 kg, 67.2 mol) in tetrahydrofuran (23.5 L) was added over 30 min while maintaining the température below 15°C. The resulting slurry was stirred at 22°C for 12 h. The presence of tertio butyl 1-(4-chloro-1 H-pyrazol-1-yl)cyclopropanecarboxylate was confirmed by HPLC analysis [HPLC rétention time: 8.76 min (Column: Halo C18,4.6 x 150 mm, 2.7pm; Mobile Phase A: acetonitrile, Mobile Phase B: 0.05% methanesulfonic acid in water; Linear Gradient: 5:95 A:B to 95:5 A:B over 9 min, then held for 1 min; Flow: 1.0 mL/min; UV détection at 210,226, and 254 nM)]. Aqueous hydrochloric acid (2M, 22.5 L, 45.0 is mol) was added while maintaining the température at 22°C. The aqueous layer was removed and the organic layer was washed with 13% aqueous sodium chloride solution (9.6 L). The wash layer was removed and the resulting solution of tert-butyl 1-(4-chloro1 H-pyrazol-1-yl)cyclopropanecarboxylate was distilled under reduced pressure at 40°C until a volume of approximately 8 L was achieved. Residual tetrahydrofuran solvents were exchanged for ethyl acetate (one 9.6-L portion, followed by continuous addition of a 19.0-L portion to maintain a constant volume) by distillation under reduced pressure at 40°C. The volume was reduced to approximately 8 L to afford a solution of tert-butyl 1(4-chloro-1H-pyrazol-1-yl)cyclopropanecarboxylate and ethyl acetate, which was used in the next step without further purification.

Step 2: 1 -(4-Chloro-1 H-pyrazol-1 -yl)cyclopropanecarboxylic acid

A solution of tert-butyl 1-(4-chloro-1H-pyrazol-1-yl)cyclopropanecarboxylate (from the previous step) In ethyl acetate (13 L, followed by a 2-L rinse) was added to a solution of anhydrous hydrogen chloride (prepared by adding acetyl chloride (9.0 L, 126 mol) to a solution of methanol (6.2 L, 154 mol) and ethyl acetate (19.0 L)) over a period of 10 min while maintaining a température of 20°C. Over a period of 12 h at that température, a precipitate formed. The presence of 1-(4-chloro-1H-pyrazol-1yl)cyclopropanecarboxylic acid was confirmed by HPLC analysis of the mixture [HPLC

116 rétention time: 5.49 min (Column: Halo C18,4.6 x 150 mm, 2.7pm; Mobile Phase A:

acetonitrile, Mobile Phase B: 0.05% methanesulfonic acid in water; Linear Gradient:

5:95 A:B to 95:5 A:B over 9 min, then held for 1 min; Flow: 1.0 mL/min; UV détection at

210, 226, and 254 nM)]. The mixture was cooled to 10°C and was held at that température for 2 h. The solids were collected by filtration, rinsïng the réaction vessel with ethyl acetate (9.6 L). The solids were dried under a nitrogen flow, and then were dried in a vacuum oven at 40°C to remove residual solvent and hydrogen chloride, to afford 1-(4-chloro-1 H-pyrazol-1-yl)cyclopropanecarboxylic acid (3.3 kg, 63% over 2 steps).

Intermediate 71: 2-(2-Hvdroxypropan-2-vl)isonicotina1dehvde

Stepl: 2-Acetylisonicotinonitrile

To a solution of isonicotinonitrile (52 g, 0.5 mol) ïn dichloromethane (1300 mL) and water (1100 mL) were added ammonium persulfate ((NH^SgOg) (170 g, 0.75 mol), silver nitrate (6.8 g, 0.04 mol) and aqueous sulfuric acid (40 mL, 98% sulfuric acid in 15 400 mL). A solution of 3-oxo-butyric acid (110 g, 1.25 mol) in dichloromethane (100 mL) was added dropwise while keeping the mixture refluxing. The reaction mixture was refluxed for 2 h. The resulting mixture was basified to pH ~8-9 using sodium carbonate powder. The mixture was filtered and the filtrate was extracted with dichloromethane (500 mL x 3). The combined organics were dried over sodium sulfate, and concentrated under reduced pressure. The residue was recrystallized from éthanol to afford 2-acetylisonicotinonitrile (52.0 g, 71.9%).

Step 2: 2-(2-Hydroxypropan-2-yl)îsonicotinonitrile

To a solution of 2-acetylisonicotinonitrile (67.1 g, 0.457 mol) in tetrahydrofuran (2400 mL) was added dropwise a solution of méthylmagnésium bromide (3.0 M, 167.6 mL, 25 0.503 mol) in toluene/tetrahydrofuran mixture (3:1 ) at -40°C over a period of 2.5 h. The reaction mixture was stirred at -40°C for another 2 h. A saturated aqueous solution of ammonium chloride (50 mL) was added. The resulting mixture was warmed to room température and another 400 mL of a saturated aqueous solution of ammonium chloride was added. The layers were separated and the aqueous layer was extracted with 30 dichloromethane (400 mL x 2). The combined organics were dried over sodium carbonate and sodium sulfate and concentrated. The residue was purified via flash chromatography (ethyl acetate/petroleum ether 1:20, 0.3% triethylamine) to afford 2-(2hydroxypropan-2-yl)isonicotinonitrile (33.0 g, 44.3%).

117

Step 3: 2-(2-Hydroxypropan-2-yl)isonicotinaldehyde

To a solution of 2-(2-hydroxypropan-2-yl)isonicotinonitrile (41.4 g, 0.255 mol) in toluene (500 mL) was added dropwise a solution of diisobutylaluminum hydride (1 mo!/L in hexane, 765 mL, 0.765 mol) at -70°C over a period of 1.5 h. The réaction mixture was stirred at -70°C for 30 min. Methanol (30 mL) was added followed by a 20% aqueous solution of sodium tartrate (700 mL) while stirring. The layers were separated and the aqueous layer was extracted with dichloromethane (400 mL x 2). The combined organics were washed with a 20% aqueous solution of sodium tartrate (700 mL). The organic phase was stirred with a 10% aqueous solution of sulfuric acid (800 mL) for 15 min. The organics were dried over sodium carbonate and sodium sulfate and concentrated under reduced pressure. A saturated aqueous solution of sodium bisulfate (400 mL) was added to the residue and the mixture was extracted with ethyl acetate (100 mL x 3). The aqueous layer was neutralized with aqueous sodium carbonate and extracted with ethyl acetate (200 mL x 3). The combined organics were dried over sodium sulfate and concentrated under reduced pressure to afford the title compound (23 g, 54%).

Intermediate 72: 2-(4-(Trifluoromethvl)-1 H-1,2.3-trlazoM -vbacetic acid

The title compound can be prepared by hydrolysis of the commercially available ethyl 2· (4-(trifluoromethyl)-1H-1,2₍3-triazol-1-yl)acetate using procedures well known in the literature.

EXAMPLES

Exampie 1 : (ffl-Pvrrolidin-1 •vlf1-(2-(3-(trifluoromethoxv)phenvl)-3Wmidazoî4.5b|pvridÎn-5-vl)piperidin-3-vl)methanone

Step 1: (/?/tert-Butyl 3-(pyrrolidine-1-carbonyl)piperidine-1-carboxylate

To a solution of pyrrolidine (2.134g, 30 mmol) in Λ/,Ν-dimethylformamide (240 mL) was added (R>1-(fert-butoxycarbonyl)piperidine-3-carboxylic acid (6.878 g, 30 mmol). Then hydroxybenzotriazole (1.028 g, 6 mmol), 1-ethyl-3-[3118 “ (dimethylamino)propyl]carbodiimide hydrochloride (5.751 g, 30 mmol), and triethylamine (7.59 g, 75 mmol) were added sequentially. The mixture was stirred at 30°C for 16 h. The solvent was removed under reduced pressure and an aqueous solution of sodium bicarbonate (200 mL, 0.1 M) was added. The mixture was extracted with ethyl acetate s (200 mL x 3) and the combined organics were dried over anhydrous sodium sulfate, filtered and concentrated under reduced pressure to afford (77)-tert-butyl 3-(pyrrolidine1-carbonyl)piperidine-1-carboxyiate, which was used in the next step without further purification.

Step 2: (fi)-PiperidÎn-3-yl(pyrrolidin-1-yl)methanone

To a solution of (fi)-tert-buty! 3-(pyrrolidine-1-carbonyl)piperidine-1-carboxyiate in methanol (120 mL) was slowly added a solution of hydrogen chloride in 1,4-dioxane (30 mL, 4M). The mixture was stirred at 30°C for 2 h. The solvent was removed under reduced pressure to afford (fi)-piperidin-3-yl(pyrrolidin-1-y!)methanone, which was used 15 in the next step without further purification.

Step 3: (fi>(1 -(6-Amino-5-nitropyridin-2-yl)piperidine-3-yl)(pyrrolidin-1 -yl)methanone To a mixture of (R>piperidin-3-yl(pyrro!ldin-1-yl)methanone in Μ,Ν-dimethylformamide (120 mL) was added diisopropyiethylamine (15 mL) followed by 6-chloro-3-nitropyridin20 2-amine (3.47 g, 20 mmol). The mixture was stirred at 50°C for 16 h. The solvent was removed under reduced pressure. The residue was purified via flash column chromatography over silica gel (ethyl acetaterpetroleum ether, 1:10 to 10:1) to afford (77/(1 -(6-amino-5-nitropyridin-2-y!)pîperidine-3-yl)(pyrro!idin-1 -yl)methanone (4.838 g, 76.3%).

Step 4: (fi/Pyrrolidin-1 -yl(l -(2-(3-(trifluoromethoxy)pheny!)-3H-imidazo[4,5-b]pyridin-5y!)piperidin-3-yl)methanone

A solution of ('fi/(1-(6-amino-5-nitropyridin-2-yl)piperidine-3-yl)(pyrrolÎdin-1y!)methanone in éthanol was prepared (0.0625 M), and 1200 pL of this solution were added into an 8 mL via! containing 3-(trif!uoromethoxy)benza!dehyde (150 pmol). Water (60 pL) and sodium hydrosulfite (65.25 mg, 375 pmol) were added under a flow of nitrogen. The via! was capped and stirred at 100°C for 16 h. The solvent was removed by Speedvac and the mixture was purified via HPLC to afford the title compound. MS API-ES+ (M+H) 460; HPLC rétention time 2.59 min (Method D).

119

The compounds listed In Table 1 below were prepared using an analogous route to the one described above for the préparation of fR>pyrrolidin-1-yl(1-(2-(3(trifluoromethoxy)phenyl)-1H-imidazo[4,5-b]pyridine-5-yl)piperidine-3-yl)methanone using the appropriate starting materials which are available commerctally or prepared using préparations well-known to those skilled in the art.

Table 1

Example	Compound Name	^R²	Analytical Data
2	(R)-(1 -(2-(4-lsobutylthiazol2-y!)-3H-imidazo[4,5b]pyridin-5-y!)piperidin-3yl)(pyrrolidin-1 yl)methanone		MS API-ES+(M+H) 439; HPLC rétention time: 2.771 min (Method D)
3	(R)-(1 -(2-(3-(1 H-Pyrazol-1 yl)phenyl)-3/+imidazo[4,5b]pyridin-5-y!)piperidin-3yl)(pyrrolidin-1yl)methanone		MS API-ES+ (M+H) 442; HPLC rétention time: 2.315 min (Method D)
4	(fî)-(1 -(2-(4-lsopropylthiazol2-yl)-3H-imidazo[4,5b]pyridin-5-y!)piperidin-3yl)(pyrrolidin-1yl)methanone		MS API-ES+ (M+H) 425; HPLC rétention time: 2.615 min (Method D)
5	( fi)-Pyrrolidin-1 -yl ( 1 -(2-mtolyl-3H-imidazo[4,5b]py ridi n-5-yi) pipe ridin-3yl)methanone		MS API-ES+ (M+H) 390; HPLC rétention time: 2.322 min (Method D)

120

Example	Compound Name	-B-R²	Analytlcal Data
6	(R)-(1-(2-(3-(Oxazol-5yl)phenyl)-3H-imidazo[4,5b]pyridin-5-yl)piperidin-3yl)(pyrrolidin-1yl)methanone	Λ N	MS API-ES+(M+H) 443; HPLC rétention time: 2.229 min (Method D)
7	(R)-(1 -(2-(5-Ch!oropyridin-3yl)-3H-lmidazo[4,5-b]pyridin5-y!)piperidin-3yl)(pyrrolidin-1yl)methanone	z=N Cl	MS API-ES+ (M+H) 411 ; HPLC rétention time: 2.46 min (Method C)
8	(fi)-/V-Methyl-3-(5-(3(pyrrolidine-1 carbonyl)piperidin-1-y!)-3Himidazo[4,5-b]pyridin-2yl) benzenesulfon amide	o^s-nh 'J ü x 0 '	MS API-ES+ (M+H) 469; HPLC rétention time: 2.4 min (Method C)
9	(R)-(1 -(2-(1 -Phenyl-1Hpyrazol-4-yl)-3Himidazo[4,5-b]pyridin-5yl)piperidin-3-yl)(pyrrolidin1-yl)methanone		MS API-ES+ (M+H) 442; HPLC rétention time: 2.384 min (Method D)
10	(fi)-Pyrro!idin-1-yl(1-(2-(2(pyrrolidin-1 -yl)pyrimidin-4yl)-3H-imidazoi4,5-b]pyridin5-yl)piperidin-3yljmethanone	N=( 0	MS API-ES+ (M+H) 447; HPLC rétention time: 2.462 min (Method C)

121

Example	Compound Name	-B-R²	Analytical Data
11	(H)-(1 -(2-(4-Methylthiazol-2yl)-3H-imidazo[4,5-b]pyridin5-yl)piperidin-3yl)(pyrrolidin-1yljmethanone		MS API-ES+ (M+H) 397; HPLC rétention time: 2.494 min (Method C)
12	(H)-(1-(2-(Pyrazolo[1,5a]pyridin-7-yl)-3Himidazo[4,5-b]pyridin-5yl)piperidtn-3-yl)(pyrrolidin1-yl)methanone	^Nô ^N \	MS API-ES+ (M+H) 416; HPLC rétention time: 2.435 min (Method D)
13	(fî)-4-(5-(3-(Pyrrolidine-1carbonyl)piperidin-1 -yl)-3Himidazo[4,5-b]pyridin-2yl)benzonitrile		MS API-ES+ (M+H) 401 ; HPLC rétention time: 2.49 min (Method C)
14	(H)-(1-(2-(2-(2- Hydroxypropan-2-yl)pyridin4-yl)-3H-imidazo[4,5b]pyridin-5-yl)piperidin-3yl)(pyrrolidÎn-1yl)methanone	*0 X^0H	MS API-ES+ (M+H) 435; HPLC rétention time: 2.442 min (Method C)
15	(H)-(1-(2-(2- (Cyclopropylamino)pyrimidin -4-yi)-3H-imidazo[4,5b]pyridin-5-yl)pÎperidin-3yl)(pyrrolidin-1yl)methanone	—o ^Nl-<	MS API-ES+ (M+H) 433; HPLC rétention time: 2.357 min (Method C)

122

Example	Compound Name	-B-R^z	Analytical Data
16	(fî)-5-(5-(3-(Pyrrolidine-1carbonyl)piperidin-1 -yl)-3 Himidazo[4,5-b]pyridin-2yl)nicotinonitrile	z-N Ή N	MS API-ES+ (M+H) 402; HPLC rétention time: 2.38 min (Method C)
17	(fî)-(1-(2-(2-Methylthiazol-4yl)-3H-imidazo[4,5-b]pyridin5-yl)piperidin-3yl)(pyrrolidin-1yl)methanone	-ZI	MS API-ES+ (M+H) 397; HPLC rétention time: 2.371 min (Method C)
18	((fî)-1-(2-((1S.2fî)-2Phenylcyclopropyl)-3Himidazo[4,5-b]pyridin-5yl)piperidin-3-yl)(pyrrolidin1-yl)methanone	-J? H	MS API-ES+ (M+H) 416; HPLC rétention time: 2.407 min (Method D)
19	( fî)-( 1 -(2-( Imidazof 1,2a]pyridin-6-yl)-3Himidazo[4,5-b]pyridin-5yl)piperidin-3-yl)(pyrrolidin1-yl)methanone		MS API-ES+ (M+H) 416; HPLC rétention time: 2.121 min (Method C)
20	(fî)-(1-(2-(4-Chloro-1methyl-1 H-pyrazol-3-yl)-3Himidazo[4,5-b]pyridin-5yl)piperidin-3-yl)(pyrrolidin1-yl)methanone	Cl	MS API-ES+ (M+H) 414; HPLC rétention time: 2.363 min (Method C)

123

Example	Compound Name		Analytlcal Data
21	(fi)-( 1 -(2-(2-Fluorophenyl)3H-imidazo[4,5-b]pyridin-5yl)piperidin-3-yl)(pyrrolidin1-yl)methanone	Ό F^Z	MS API-ES+ (M+H) 394; HPLC rétention time: 2.199 min (Method D)
22	(fi)-(1-(2-(2-Phenylpropan2-yl)-3H-imidazo[4,5b]pyridin-5-yl)piperidin-3yl)(pyrrolidin-1yl)methanone	T	MS API-ES+ (M+H) 418; HPLC rétention time: 2.365 min (Method D)
23	(fi)-(1-(2-(2-Hydroxy-3Îsopropylphenyl)-3Himidazo[4,5-b]pyridin-5yl)plperidin-3-yl)(pyrrolidin1-yl)methanone	—O HcQ—	MS API-ES+ (M+H) 434; HPLC rétention time: 2.794 min (Method D)
24	(R)-(1-(2-iert-Butyl-3Himidazo[4,5-b]pyridin-5yl)piperidin-3-yl)(pyrrolidin1-yl)methanone	K	MS API-ES+ (M+H) 356; HPLC rétention time: 2.351 min (Method C)
25	(fi)-(1-(2-(2- Morpholinopyrimidin-4-yl)3H-imidazo[4,5-b]pyridin-5yl)piperidin-3-yl)(pyrrolidin1-yl)methanone	-Q N—\ Q	MS API-ES+ (M+H) 463; HPLC rétention time: 2.504 min (Method C)

124

Example	Compound Name	HTr²	Analytlcal Data
26	( fi)-( 1 -(2-Cyclohexyl-3H· imidazo[4,5-b]pyridin-5yl)piperidin-3-yl)(pyrrolidin1-yl)methanone	*O	MS API-ES+ (M+H) 382; HPLC rétention time: 2.278 min (Method D)
27	(fî)-6-(5-(3-(Pyrrolidine-1 carbonyl)piperidin-1 -yl)-3H· imidazo[4,5-b]pyridin-2-yl)2H-benzo[b][1,4]oxazin3(4H)-one	0	MS API-ES+ (M+H) 447; HPLC rétention time: 2.353 min (Method C)
28	(R)-(1-(2- (Cyclohexylmethyl)-3H· imidazo[4,5-b]pyridin-5yl)piperidîn-3-yl)(pyrrolidin1-yl)methanone		MS API-ES+ (M+H) 396; HPLC rétention time: 2.444 min (Method D)
29	( fi)-( 1 -(2-Cyclopropyl-3H· imidazo[4,5-b]pyridin-5yl)pîperidtn-3-yl)(pyrrolidin· 1-yl)methanone		MS API-ES+ (M+H) 340; HPLC rétention time: 2.246 min (Method C)
30	(fi)-N-(3-(5-(3-(Pyrrolidine- 1 -carbonyl)piperidin-1 -yl)3H-imidazo[4,5-b]pyridin-2yl)phenyl) methanesulfonamide	,p ^ΗΝΛ	MS API-ES+ (M+H) 469; HPLC rétention time: 2.388 min (Method C)

125

Example	Compound Name	-B-R²	Analytlcal Data
31	(fî)-(1-(2-(tertButoxymethyl)-3Himldazo[4,5-bJpyridin-5y1)pïperidin-3-y1)(pyrro1idin1-yl)methanone		MS API-ES+ (M+H) 386; HPLC rétention time: 2.453 min (Method C)
32	(fî)-(1 -(2-(5-Ethylisoxazol-3yl)-3H-imidazo[4,5-bJpyridin5-yl)piperidin-3yl)(pyrrolidin-1yljmethanone		MS API-ES+ (M+H) 395; HPLC rétention time: 2.551 min (Method C)
33	(fî)-(1 -(2-(5-Methylpyridin-3y I) -3 H-imidazo[4,5-b] pyridi n5-yl)piperidin-3yl)(pyrrolidin-1yl)methanone	z=N	MS API-ES+ (M+H) 391; HPLC rétention time: 2.224 min (Method C)
34	(fî)-Pyrrolidin-1 -y I ( 1 -(2-(4(trifluoromethoxy)phenyl)3H-imidazo[4,5-b]pyridin-5yl)piperidin-3-yl)methanone	F	MS API-ES+ (M+H) 460; HPLC rétention time: 2.567 min (Method D)
35	(fî)-(1 -(2-(3-Fluorophenyl)3H-imidazo[4,5-b]pyridin-5yl) pi peridin-3-yl) (pyrrolidi n1-yl)methanone	F	MS API-ES+ (M+H) 394; HPLC rétention time: 2.263 min (Method D)

126

Example	Compound Name	-B-R²	Analytlcal Data
36	(H)-(1-(2-(3- Methoxyphenyl)-3Himldazo[4,5-b]pyridin-5yl)piperidin-3-yl)(pyrrolidin1-yl)methanone	-q /	MS API-ES+ (M+H) 406; HPLC rétention time: 2.279 min (Method D)
37	(fî)-(1 -(2-(6-Methoxypyridin2-yl)-3H-imidazo[4,5b]pyridin-5-yl)piperidin-3yl)(pyrrolidin-1yl)methanone	-q /	MS API-ES+ (M+H) 407; HPLC rétention time: 2.304 min (Method D)
38	(H)-Pyrrolidin-1 -y I ( 1 -(2-(6(pyrrolidin-1 -y I) py ridi n-2-y I)3H-imidazo[4,5-b]pyridin-5yl)piperidin-3-yl)mettianone	-o 0	MS API-ES+ (M+H) 446; HPLC rétention time: 2.525 min (Method D)
39	(fî)-(1 -(2-(2-Phenyloxazol-4yl)-3H-imidazo[4,5-b]pyridin5-yl)piperidin-3yl)(pyrrolidin-1yl)methanone	“K	MS API-ES+ (M+H) 443; HPLC rétention time: 2.477 min (Method D)
40	(fî)-(1 -(2-(4-Ethylthiazol-2yl)-3H-imidazo[4,5-bJpyridin5-yl)piperidtn-3yl)(pyrrolidîn-1yljmethanone	Kl	MS API-ES+ (M+H) 411 ; HPLC rétention time: 2.437 min (Method D)

127

Example	Compound Name	-B-R²	Analytical Data
41	(R)-(1 -(2-(3-Chlorophenyl)3H-imidazo[4,5-b]pyridin-5yl)piperidin-3-yl)(pyrrolidin1-yl)methanone	-n Cl	MS API-ES+ (M+H) 410; HPLC rétention time: 2.389 min (Method D)
42	(R)-(1 -(2-(4- fert-B utylthiazol2-yl)-3H-imidazo[4,5b]pyridin-5-yl)piperidin-3yl)(pyrrolidin-1yl)methanone	-<^si N-jy-	MS API-ES+ (M+H) 439; HPLC rétention time: 2.786 min (Method D)

Example 43: (R)-(1-(2-(3-(Difluoromethoxv)phenvl)-3H-imidazof4,5-blpvridin-5vl)piperidln-3-v1)(pvrrolîdln-1-vl)methanone

Step 1: (fî/fert-Butyl 3-(pyrrolidine-1-carbonyl)piperidine-1 -carboxylate (fî/tert-Butyl 3-(pyrrolidine-1-carbonyl)piperidine-1-carboxylate was prepared by an anaiogous procedure to the one used for Example 1, Step 1.

Step 2: (R>Piperidin-3-yl(pyrrolidin-1-yl)methanone

Into a flask containing (R}·fert-butyl 3-(pyrrolidine-1-carbonyl)piperidine-1-carboxy late was added dichioromethane (24 mL) followed by a solution of hydrogen chloride in 1,4* dioxane (4M, 8 mL). The reaction mixture was stirred at 30°C for 2 h. The solvent was evaporated under reduced pressure to afford (R)-piperidin-3-yl(pyrrolidin-115 yl)methanone which was used without further purification.

Step 3: (R>(1 -(6-Amino-5-nitropyridin-2-yl)plperidine-3-yl)(pyrrolidin-1 -yl)methanone

A solution of 6-chloro-3-nitropyridin-2-amlne (0.175M, 20 mL, 3.5 mmol) in anhydrous

Λ/,Ν-dimethylformamide was added to (R>piperidin-3-yl(pyrrolidin-1-yljmethanone,

128

followed by diisopropyethylamine (1.3 mL, 7.0 mmol). The réaction mixture was stirred at 80°C for 16 h. The solvent was removed under reduced pressure and the residue was purified via préparative HPLC to afford (R)-(1-(6-amino-5-nitropyridin-2yl)piperidine-3-yl)(pyrrolidin-1-yljmethanone (1.0 g, 89.6%).

Step 4: (fî)-(1 -(2-(3-(Difluoromethoxy)phenyl)-3H-imidazo[4,5-b]pyridin-5-yl)piperidin-3yl) (pyrrolidin-1 -yljmethanone

The title compound was prepared by a method analogous to the one used for Example

1, Step 4, but the reaction mixture was stirred at 110°C. MS API-ES+ (M+H) 442;

HPLC rétention time: 2.431 min (Method D).

The compounds listed in Table 2 below were prepared using an analogous route to the one described above for the préparation of (fî)-(1-(2-(3-(difluoromethoxy)phenyi)-3Himidazo[4,5-blpyridin-5-yl)piperidin-3-yl) (pyrrolidin-1-yljmethanone using the appropriate starting materials which are available commerciaily or prepared using préparations well15 known to those skilled ln the art.

Table 2

Example	Compound Name	-B-R²	Analytical Data
44	(R)-(1 -(2-(6-Methylpyridin-2yl)-3H-lmidazo[4,5-bJpyridin-5yl)piperidin-3-yl) (pyrrolidin-1 yljmethanone		MS API-ES+ (M+H) 391 ; HPLC rétention time: 2.442 min (Method C)
46	(fî)-(1 -(2-(4-Chlorobenzyl)-3H· imldazo[4,5-b]pyridin-5yl)piperidin-3-yl)(pyrrolidin-1 yljmethanone	Cl	MS API-ES+ (M+H) 424; HPLC rétention time: 2.445 min (Method D)

129

Example	Compound Name	HTr²	Analytical Data
47	(H)-3-(5-(3-(Pyrrolidine-1 carbonyl)piperidin-1 -yl)-3Himidazo[4,5-b]pyridin-2yl)benzonitrile	Ή N	MS AP1-ES+ (M+H) 401; HPLC rétention time: 2.489 min (Method C)

Example 48: ffl)-(1-i-2-((4-Chlorophenoxv)methvlÎ-3H-imidazof4.5-b]pvridîn-5vnplperidin-3-vl)(pvnOlidîn-1-vDmethanone

Step 1 : -(6-Amino-5-nitropyridin-2-yl)piperidin-3-yl)pyrrolidin-1 -yl)methanone

To a solution of (fi>piperidin-3-yl(pyrrolidin-1-yl)methanone in /V,/V-dimethylformamide (16.3mL, 0.6M) was added a solution of 6-chloro-3-nitropyridin-2-amîne in N,Ndimethylformamide (16.3mL, 0.6M). Then triethylamine (3.68mL, 26.5 mmol) was added to the mixture. The reaction mixture was stirred at 80°C for 16 h. The solvent was removed under reduced pressure to afford (R)-(1-(6-amino-5-nitropyridin-2yl)piperidin-3-yl)pyrrolidin-1-yl)methanone and the crude product was used without further purification.

Step 2: (fi>(1-(5_t6-Diaminopyridin-2-yl)piperldin-3-yl)(pyrrolidin-1-yl)methanone

To a solution of (/?}-(1-(6-amino-5-nitropyridin-2-yl)pipendin-3-yl)pyrrolidin-1yl)methanone in methanol (9.8 mmol, 0.25M) was added zinc dust (6.37 g, 98 mmol) at 0°C followed by a saturated aqueous solution of ammonium chloride (39.2 mL). The reaction mixture was stirred at 30°C for 16 h. The mixture was filtered and concentrated under reduced pressure. Water (100 mL) was added to the residue and the mixture was extracted with ethyl acetate (200 mL x 3). The combined organics were dried over anhydrous sodium sulfate, filtered and concentrated under reduced pressure to afford

-(5,6-diaminopyridin-2-yl)piperidin’3-yl)(pyrrolidin-1 -yl)methanone. The material was used for the next step without further purification.

130

Step 3: (Ή>(1 -(-2-((4-Chlorophenoxy)methyl)-3H-imidazo[4,5-b]pyridin-5-yl)piperidin-3yl)(pyrrolidin-1 -yl)methanone

2-(4-Chlorophenoxy)acetic acid (75 pmol) was added into an 8-mL vial followed by a solution of (fî>(1-(5,6-diaminopyridÎn-2-yl)piperidin-3-yl)(pyrrolidin-1-yl)methanone in anhydrous dioxane (600 pL, 127.5 pmol, 0.21M). Triethylamine (90 pL, 648 pmol) and 1-propanephosphonic acid cyclic anhydride (120 pL, 50% in ethyl acetate, 202 pmol) were added. The vial was capped and the reaction mixture was shaken at 140°C for 16 h. The solvent was removed by Speedvac and the residue was purified via HPLC to afford the title compound. MS API-ES+ (M+H) 440; HPLC rétention time 2.473 min (Method D).

The compounds listed in Table 3 below were prepared using the route described above for the préparation of Example 48 using the appropriate starting materials which are available commerciaily or prepared using préparations well-known to those skilled in the 15 art.

Table 3

Example	Compound Name	-B-R²	Analytlcal Data
49	(fl)-(1 -(2-(2,3-Dihydro-1 Hinden-2-yl)-3H-imidazo[4,5b]pyridin-5-yl)piperidin-3yl)(pyrrolidin-1 -yl)methanone	-co	MS API-ES+ (M+H) 416; HPLC rétention time: 2.364 min (Method D)
50	(fî)-(1 -(2-(2-Chlorobenzyl)3H-imidazo[4,5-b]pyridin-5yl)piperidin-3-yl)(pyrrolidin-1 · yljmethanone		MS API-ES+ (M+H) 424; HPLC rétention time: 2.314 min (Method D)

131

Example	Compound Name	-B-R^a	Analytical Data
51	(H)-(1 -(2-(1- Phenylcyclopropyl)-3Himidazo[4,5-b]pyridin-5yl)piperidin-3-yl)(pyrrolidin-1yl)methanone	T	MS API-ES+ (M+H) 416; HPLC rétention time: 2.344 min (Method D)
52	(fî)-(1 -(2-((1 H-Pyrazol-1 yl)methyl)-3H-imidazo[4,5blpyridin-5-yl)piperidin-3yl) (pyrrolidin-1 -yljmethanone	A _yN-N	MS API-ES+ (M+H) 360; HPLC rétention time: 2.162 min (Method C)
53	(H)-(1-(2-Cyclobutyl-3Hîmidazo[4,5-blpyridÎn-5yl)piperidin-3-yl)(pyrrolidin-1 yljmethanone	-0	MS API-ES+ (M+H) 354; HPLC rétention time: 2.301 min (Method C)
54	(H)-/V-((5-(3-(Pyrrolidine-1carbonyl)piperidin-1 -yl)-3Himidazo[4,5-blpyridin-2yljmethyljbenzamide	_ZNH	MS API-ES+ (M+H) 433; HPLC rétention time: 2.381 min (Method C)
55	(R)-Pyrrolidin-1 -yl( 1 -(2-((4(trifluoromethylJ-1 H-1,2,3triazol-1 -yl)methyl)-3Himidazo[4_t5-blpyridin-5yl)piperidin-3-yl)methanone	F c X	MS API-ES+ (M+H) 449; HPLC rétention time: 2.488 min (Method C)
56	(H)-(1 -(2-((2-Cyclopropyl-5methyloxazol-4-yl)methyl)3H-imîdazo[4,5-blpyridin-5yl)piperidin-3-yl)(pyrrolidin-1yljmethanone	ï N^p	MS API-ES+ (M+H) 435; HPLC rétention time: 2.487 min (Method C)

132

Example 57: (HI-6-Methvl-1-i(5-i3-fpvrrolÎdÎne-1-carbonvDpiperidin-1-vl)-3H· lmldazor4,5-bÎPvridÎn-2-vDmethvl)pyridin-2(1H)-one

Step 1 : (fi)-M-(2-Amino-6-(3-(pyrrolidine-1-carbonyl)piperidin-1-yl)pyridin-3-yl)-6m et hyl-2-oxopyridine-1 (2 H)-ca rboxamlde

Into a vial were added ('fî)-(1-(5,6-diamÎnopyridin-2-yl)plperidin-3-yl)(pyrrolidin-1y1)methanone (100 pmol), 6-methyl-2-oxopyridine-1(2H)-carboxylic acid and N,Ndimethylformamide (1.5 mL) followed by 0-(7-Azabenzotriazol-1-yl)-N,N,N’,N',tetramethyluronlum hexafluorophosphate (HATU) (200 pmol) and triethylamine (250 pmol). The vial was shaken for 16 h at room température. The mixture was concentrated via Speedvac. The residue was partitioned between dichloromethane and water. The organlcs were evaporated under vacuum to afford (fi)-M-(2-amino-6-(3(pyrrolidine-1 -carbony1)piperidin-1 -yl)pyridin-3-yl)-6-methyl-2-oxopyridine-1 (2H)carboxamlde which was used without further purification.

Step 2: (H)-6-Methyl-1-((5-(3-(pyrro1idine-1 -carbony1)pÎperidin-1-yl)-3H-imidazo[4,5-

b]pyridin-2-y1)methy1)pyridin-2(1H)-one

Into a vial were added (fi)-/\A(2-amino-6-(3-(pyrrolidine-1-carbonyl)piperidin-1-yl)pyridin-

3-y1)-6-methyl-2-oxopyridine-1 (2H)-carboxamide (1 equiv) and a solution of methanol and isobutanol (1:1,1.5 mL). Sodium methoxide (2.0 equiv) was added and the reaction mixture was shaken at 110°C for 2 h. Water was added and the mixture was extracted with a mixture of methanol/dichloromethane (1:1). The organics were concentrated by Speedvac and the crude material was purified via préparative HPLC to afford the title compound. MS (ES+) (M+H) 421.4; UPLC 1.09 min (Method T).

133 ® Example 58: Îfî)-(1-(2-(1-(1H-Pvrazol-1-vnethvl)-3H-imldazof4.5-b1pvridin-5vl)piperidin-3-vl)(pvrrolidîn-1-vDmethanone

The title compound was prepared by a method analogous to the one used for Example 5 57. MS (ES+) (M+H) 394.5; UPLC rétention time: 1.1 min (Method T).

Example 59: 2-(2-CvclopropvlpvrimÎdin-4-vl)-5-i3-(pvridin-2-vl)piperidin-1 -vl)-3H· îmldazof4,5-blpvridine

Step 1: 2-Amîno-6-(3-(pyridin-2-yl)piperidin-1-yl)nicotinicacid

To a solution of 2-amlno-6-chloronlcotinic acid (1.6 g, 9.248 mmol) in N,Mdimethylformamide (41 mL) was added 2-(piperidin-3-yl)pyridine (1.82 g, 11.098 mmol) and triethylamine (25.455 mmol). The réaction mixture was stirred at 80°C for 16 h. The solvent was removed under reduced pressure and the residue was purified via column chromatography to afford 2-amîno-6-(3-(pyridin-2-yl)piperidin-1 -yl) nicotinlc acid

Step 2: 2-(2-Cyclopropylpyrimidin-4-yl)-5-(3-(pyridin-2-yl)plperidin-1 -yl)-3H-imidazo[4,5bjpyridine

Into a vial containing 2-cyclopropylpyrimidine-4-carbaldehyde was added 2-amîno-6-(320 (pyridin-2-yl)piperidin-1 -yljnicotinle acid (500 pL, 0.33M in éthanol) followed by éthanol (500 pL), water (50 pL), and a solution of sodium hydrosulfite (100 mg, 574 pmol) in water (50 pL). The vial was capped and shaken at 110°C for 18 h. The mixture was filtered and the filtrate was concentrated by Speedvac. The residue was purified by HPLC to afford the title compound. MS (API-ES+) (M+H) 398.3; UPLC rétention time: 25 1.15 min (Method U)

Example 60: (R)-1 -(2-(3-(Difluoromethoxv)phenvl)-3H-imidazoI4.5-b1pyridin-5-vl)-N.NdÎmethylpîperidine-3-carboxamide

134

Step 1 : (R)-1 -(6-Amino-5-nitropyridin-2-yl)-/V,/V-dimethylplperidine-3-carboxamlde

To a solution of 6-chloro-3-nitropyridin-2-amine (4.15 g, 24.0 mmol) in Ν,Νdimethylformamide (41 mL) was added (fî)-A/,A/-dimethylpiperidîne-3-carboxamide (3.74 g, 2.40 mmol) followed by triethylamine (10.7 mL, 64.800 mmol). The reaction mixture was stirred at 80°C for 16 h. The solvent was removed under reduced pressure and the residue was purified via column chromatography to afford (fi)-1-(6-amino-5-nitropyridin-

2-yl)-/V,W-dimethylpiperidine-3-carboxamide.

Step 2: (7î)-1-(2-(3-(Difluoromethoxy)phenyl)-3H-imîdazo[4,5-b]pyridin-5-yl)-/V,/Vdimethylpiperidine-3-carboxamide

Into a 4 mL vial was added (fi>1-(6-amlno-5-nitropyridin-2-yl)-/V,/V-dimethylplperidine-3carboxamide (500 pL, 0.3M) dissolved in AZ,A/-dïmethylformamide followed by 3(difluoromethoxy)benzaldehyde (950 pL, 0.15ΘΜ) dissolved in A/.W-dimethylformamide.

Water (50 pL) was added followed by sodium hydrosulfite (574 pmol) under a nitrogen atmosphère. The vial was capped and shaken at 110°C for 18 h. The solvent was removed by Speedvac and the residue was purified by HPLC to afford the title compound. MS (API-ES+) (M+H) 416.2; HPLC rétention time 1.392 min (Method E).

Example 61 : (ffl-fco-Butvl 3-(5-(3-(Dvrrolldine-1 -carbonvl)pÎperidin-1 -vh-3Himidazor4.5-blDvridine-2-vl)piperidine-1-carboxylate

Step 1: tert-Butyl 3-((2-amino-6-((flJ-3-(pyrrolidine-1-carbonyl)piperidin-1-yl)pyridin-3yl)carbamoyl)piperidine-1-carboxylate.

To a solution of (fi>(1-(5,6-diaminopyridin-2-yl)pÎperidin-3-yl)(pyrrolidin-1-yl)methanone (3.60 mmol) in 1,4-dioxane (20 mL) was added (fi)-1-(tert-butoxycarbonyl)piperidine-3carboxylic acid (3.60 mmol), 1-Propanephosphonîc acid cyclic anhydride (10.8 mmol),

135 and triethylamine (32.4 mmol). The reaction mixture was stirred at room température for 16 h. The solvent was removed under reduced pressure and a solution of methanol/dichloromethane (1:9,50 mL) was added followed by a saturated aqueous solution of sodium bicarbonate (50 mL), and the layers were separated. The aqueous layer was extracted again with methanol/dichloromethane (1:9,25 mL) and the combined organics were dried over anhydrous sodium sulfate, filtered and concentrated under reduced pressure to afford tert-butyl 3-((2-amino-6-(('R}-3-(pyrrolidine-1carbonyl)pïperidin-1-yl)pyridin-3-yl)carbamoy!)piperidine-1-carboxylate which was used without further purification.

Step 2: (fî/tert-Butyl 3-(5-(3-(pyrrolidine-1-carbonyl)piperldin-1-yl)-3H-imidazo[4,5b]pyridine-2-yl)piperidine-1-carboxylate

To a solution of tert-butyl 3-((2-amino-6-((fl>3-(pyrrolidine-1-carbonyl)piperidin-1yl)pyridin-3-yl)carbamoyl)piperidine-1-carboxylate in methanol/seobutanol (1:2,15 mL) was added a solution of 25% sodium methoxide in methanol (9.0 mL). The reaction mixture was stirred at 90°C for 16 h. The solvent was removed under reduced pressure and a solution of methanol/dichloromethane (1:9,50 mL) and a saturated aqueous solution of sodium bicarbonate (50 mL) were added to the residue, and the layers were separated. The aqueous layer was extracted again with methanol/dichloromethane (1:9,25 mL). The combined organics were dried over sodium sulfate, filtered and concentrated under reduced pressure. The residue was purified via column chromatography to afford tert-butyl 3-(5-(3-(pyrrolidine-1-carbonyl)piperidin-1-yl)-3Himidazo[4,5-b]pyridÎne-2-yl)piperidine-1-carboxylate.

Step 3: -(S-tPiperidin-S-yO-SH-Îmidazo^.S-bJpyridin-S-ylJpiperidin-S-yOpyrrolidin1-yl)methanone

A solution of hydrogen chloride in 1,4-dioxane (4M) was added to (H}-tert-butyl 3-(5-(3(pyrrolidine-1 -carbonyl)piperidin-1 -yl)-3H-îmidazo[4,5-b]pyridine-2-yl)piperidine-1 carboxylate to préparé a 1M solution. The reaction mixture was stirred at room température for 4 h. The solvent was removed under reduced pressure to afford (Rffl~ (2-(piperidin-3-yl)-3H-imidazo[4,5-b]pyridin-5-yl)piperidin-3-yl)pyrrolidin-1-yl)methanone which was used without further purification.

136

Step 4: (fî)-/so-Butyl 3-(5-(3-(pyrrolidine-1 -carbonyl)piperidïn-1 -yl)-3H-imidazo[4,5b]pyridine-2-yl)piperidine-1-carboxylate

To a solution of (fî}-(1-(2-(pÎperidin-3-yl)-3H-imidazo[4,5-b]pyridin-5-yl)piperidin-35 yl)pyrrolidin-1-y1)methanone (500 μΙ_, 150 pmol, 0.13M) in Μ,Μ-dimethylformamide was added a solution of isobutyl chloroformate in tetrahydrofuran (0.3M, 500 μΙ_, 150 pmol)followed by diisopropylethylamine (450 pmol). The réaction mixture was stirred at room température for 16 h. The solvent was removed and to the residue was added a solution of methanol/dichloromethane (1:9, 50 mL) and a saturated aqueous solution of 10 sodium bicarbonate (50 mL). The aqueous layer was removed and the organics were concentrated under reduced pressure. The residue was purified via HPLC to afford the title compound. MS (ES+) (M+H) 483; LCMS rétention time 1.11 min (Method V).

The compounds listed in Table 4 below were prepared using the route described above for the préparation of Example 61 using the appropriate starting materials which are available commercially or prepared using préparations well-known to those skilled In the art.

Table 4

Example	Compound Name	^R²	Analytlcal Data
62	(R)-(1-(2-(1(Cyclopentanecarbonyl)piperidin3-yl)-3H-imidazo[4,5-b]pyridin-5yl)piperidin-3-yl)(pyrrolidin-1 y1)methanone	/—N	MS (ES+) (M+H) 479; UPLC rétention time: 1.05 min (Method V)
63	(R)-(1-(2-(1(Cyclopentanecarbonyl)piperidin4-yl)-3H-imldazo[4,5-b]pyridin-5yl)piperidin-3-yl) (pyrrolid in-1 yl)methanone	-n	MS (ES+) (M+H) 479; UPLC rétention time: 1.02 min (Method V)

137

Example

Compound Name

Analytical Data (fî)-(1 -(2-(1(Propylsulfony!)piperidin-3-yl)3M-imidazo[4,5-b]pyridin-5yl)plperid in-3-y I) (py rrol idin-1 yl)methanone

MS (ES+) (M+H) 489; UPLC rétention time: 1.02 min (Method V)

Exampie 65: ((/7)-1-(2-(2-Cvclopropvlpvrimidin-4-vl)-3ffimidazor4.5-b|pyridin-5vl)plperidin-3-vl)((S)-3-fluoropvrrolidin-1-vl)methanone

Step 1: (/7)-Piperidïne-3-carboxylic acid

To (R/1-(tert-butoxycarbonyl)pîperidine-3-carboxylic acid (7.0 mmol) was added dichloromethane (48 mL) followed by hydrochloric acid (4M, 16 mL). The reaction mixture was stirred at 30°C for 2 h. The solvent was removed under reduced pressure to afford (/7)-piperidine-3-carboxylic acid which was used without further purification.

Step 2: (fl)-1 -(6-Amino-5-nitropyridin-2-yl)piperidine-3-carboxylic acid

A solution of 6-chloro-3-nitropyridin-2-amine (0.175 M, 35 mL, 6.125 mmol) was added to (fl)-piperidine-3-carboxylic acid, prepared during the previous step, followed by diisopropylethyl amine (2.275 mL, 12.25 mmol). The reaction mixture was stirred at 80°C for 16 h. The solvent was removed under reduced pressure to afford (/7)-1-(6amino-5-nitropyridin-2-yl)piperidine-3-carboxylic acid which was used for the next step without further purification.

Step 3: (fl)-1 -(2-(2-Cyclopropylpyrimidin-4-yl)-3W-imidazo[4,5-b]pyridin-5-yl)piperidine-

3-carboxylic acid (/7)-1-(6-amino-5-nitropyridin-2-yl)piperidine-3-carboxylic acid, prepared during the previous step, was disolved in a mixture of N./V-dimethylformamide (45.5 mL) and water (4.9 mL). Ammonium formate (1.925 g, 30.625 mmol) was added followed by zinc dust (1.05 g, 15.3 mmol). The reaction mixture was stirred at 45°C for 16 h. The mixture

138 was filtered and the filtrate was concentrated under reduced pressure. To the residue was added /V.N-dimethylformamîde (24.5 mL) followed by a solution of 2cyclopropylpyrimidine-4-carbaldehyde in Λ/,/V-dimethylformamide (24,5 mL, 6.125 mmol,

0.25M) and acetic acid (3.675 mL, 61.25 mmol). The reaction mixture was stirred at

60°C for 16 h. The solvent was removed under reduced pressure and the residue was purified via préparative HPLC to afford the title compound (0.637 g, 28.6%).

Step 4: ((R)-1 -(2-(2-Cyclopropyipyrimidin-4-yl)-3H-imidazo[4,5-b]pyridin-5-yl)piperidin3-yl)((S)-3-fiuoropynOlidin-1-yl)methanone (S)-3-Fluoropyrroiidine (175 pmol) was added to a vial followed by N,Ndimethylformamide (200 pL), triethylamine (35 pL, 250 pmol) and ( R)-1-(2-(2cyciopropylpyrimidin-4-yl)-3H-imidazo[4,5-b]pyridin-5-yl)piperidine-3-carboxylicacid (200 pL, 50 pmol, 0.25 M in A/,A/-dimethylformamide) and 0-(7-azabenzotriazol-1-yl)Ν,Ν,Ν’,Α/',-tetramethyluronium hexafluorophosphate (HATU) (250 pL, 100 pmol, 0.4 M solution in A/,A/-dimethylformamide). The reaction mixture was shaken at 50°C for 16 h. The solvent was evaporated by Speedvac and the residue was purified via préparative HPLC to afford the title compound. MS API-ES+ (M+H) 436; HPLC rétention time 2.521 min (Method C).

The compounds listed in Table 5 below were prepared using procedures analogous to those described above for the synthesis of Example 65 using the appropriate starting materials which are available commercially or prepared using préparations well-known to those skilled in the art or prepared by a route described above.

139

Table 5

Example	Compound Name	R	Analytical Data
66	((fi)-1 -(2-(2-Cyclopropylpyrimidin4-yl)-3H-imidazo[4,5-b]pyridin-5yl)piperidin-3-yl)((fi)-2(hydroxymethyl)pyrrolidin-l yljmethanone	_Z°^Hà'	MS API-ES+ (M+H) 448; HPLC rétention time: 2.431 min (Method C)
67	((fi)-1-(2-(2-Cyclopropylpyrimidin4-yl)-3H-imidazo[4,5-b]pyridin’5yl)pïperidin-3-yl)((fi)-2methylpyrrolidin-1 -yl)methanone	b'	MS API-ES+ (M+H) 432; HPLC rétention time: 2.694 min (Method C)
68	( ( fi)-1 -(2-(2-Cyclopropylpyrimidin4-yl)-3H-imidazo[4,5-b]pyridin-5yl)piperidin-3-yl)((3fî,4fi)-3,4difluoropyrrolidin-1-yl)methanone	F	MS API-ES+ (M+H) 454; HPLC rétention time 2.579 min (Method C)
69	((fi)-1-(2-(2-Cyclopropylpyrimidin4-yl)-3H-lmidazo[4,5-b]pyridin-5yl) pipe ridin-3-yl) (( fi)-3fluoropyrrolidin-1 -yl)methanone	'-cr	MS API-ES+ (M+H) 436; HPLC rétention time 2.531 min (Method C)
70	(fi)-(1-(2-(2-Cyclopropylpyrimidin4-yl)-3H-lmidazo[4₁5-b]pyridin-5yl)piperidin-3yl)(morpholino)methanone	M	MS API-ES+ (M+H) 434; HPLC rétention time 2.435 min (Method C)

140

Example 71 : /77)-1 -(2-(2-CvcloDroDvlDvrimidin-4-vlV3H-imidazo|'4,5-blDvridin-5-vD-/V./Vdimethvlpiperidine-3-carboxamide

Step 1 : (77)-Piperidine-3-carboxylic acid

To a solution of (77>1-(tert-butoxycarbonyl)piperidine-3-carboxylic acid (12.25 mmol) in dichloromethane (34 mL) was added a solution of hydrogen chloride in 1,4-dioxane (28 mL, 4M). The reaction mixture was stirred at 30°C for 2 h. The solvent was removed under reduced pressure to afford (77)-piperidine-3-carboxylic acid which was used without further purification.

Step 2: (/7)-1-(6-Amino-5-nitropyridin-2-yl)piperidine-3-carboxylic acid

A solution of 6-chloro-3-nitropyridin-2-amine in Μ,Μ-dimethylformamide (70 mL, 12.25 mmol, 0.175M) was added to ('/7_i)-piperidÎne-3-carboxylic acid (12.25 mmol) followed by diisopropylethylamine (4.55 mL, 24.5 mmol). The reaction mixture was stirred at 80°C for 16 h. The solvent was removed under reduced pressure to afford (R)-} -(6-amino-5nitropyridin-2-yl)piperidine-3-carboxylic acid which was used without further purification.

Step 3: (R>1-(5,6-Diaminopyridin-2-yl)piperidine-3-carboxylic acid

To a solution of (77)-1-(6-amino-5-nitropyridin-2-yl)piperidine-3-carboxylic acid in N,Ndimethylformamide (91 mL) was added water (9.8 mL), ammonium formate (3.85 g, 61.25 mmol), and actived zinc dust (2.1 g, 30.6 mmol) under a nitrogen atmosphère. The reaction mixture was stirred at 45°C for 16 h. The mixture was filtered and concentrated under reduced pressure to afford (/?)-1-(5_l6-diaminopyridin-2-yl)piperidine3-carboxylic acid which was used without further purification.

Step 4: (R)-1 -(2-(2-Cyclopropylpyrimidin-4-yl)-3H-îmidazoi4₁5-b]pyridin-5-yl)piperidine3-carboxylic acid

To a solution of (R/-1 -(5,6-diamînopyridin-2-yl)piperidine-3-carboxylîc acid in N,Ndimethylformamide (49 mL, 12.25 mmol, 0.25M) was added a solution of 2cyclopropylpyrimidine-4-carbaldehyde in N,W-dimethylformamide (49 mL, 12.25 mmol, 0.25M) followed by acetic acid (7.35 mL, 122.5 mmol). The reaction mixture was stirred at 60°C for 16 h. The solvent was removed under reduced pressure and the residue

141 was purified via HPLC to afford (7?>1-(2-(2-cyclopropylpyrimidin-4-yl)-3H-imidazo[4,5b]pyridin-5-yl)piperidine-3-carboxylic acid.

Step 5: (7?>1 -(2-(2-Cyclopropylpyrimidin-4-yl)-3H-imidazo[4,5-b]pyridin-5-yl)-N,Ndimethylpiperidine-3-carboxamide

To a solution of dimethylamine (175 pmol) in A/,A/-dimethylformamide (200 pL) was added triethylamine (35 pL, 250 pmol) and (fî>1-(2-(2-cyclopropylpyrimidin-4-yl)-3Himidazo[4,5-b]pyridin-5-yl)piperidine-3-carboxylic acid (200 pL, 50 pmol, 0.25M in N,Ndimethylformamide) followed by 2-(1H-7-azabenzotriazol-1-yl)-1,1,3,3-tetramethyl uronium hexafluorophosphate (250 pL, 100 pmol, 0.4M in ty/V-dimethylformamide). The reaction mixture was stirred at 50°C for 16 h. The solvent was removed by Speedvac and the residue was purified via HPLC to afford the title compound. MS APIES+ (M+H) 392; HPLC rétention time 2.43 min (Method C).

Exampie 72: (fî)-(1-(2-(1-Î4-Methvl-1 H-pvrazol-1-vl)cvcloDropvlÎ-3Wmidazof4,5b1pvridin-5-vnpiperidin-3-vl)Dvrrolidin-1-vl)methanone

Into a 5 mL microwave vial were added (fî>(1-(5,6-diaminopyridin-2-yl)piperidin-3yl)(pyrrolidin-1-yl)methanone dihydrochloride (Intermediate 1) (296 mg, 0.814 mmol) and 1-(4-methyl-1H-pyrazol-1-yl)cyclopropanecarboxy!ic acid (Intermediate 3) (137.5 mg, 0.827 mmol) and pyridine (2 mL). The vial was capped and triphenylphosphite (0.650 mL, 2.48 mmol) was added. The reaction mixture was stirred at 200°C in a microwave for 10 min. To the mixture was added ethyi acetate and a saturated aqueous solution of ammonium chioride. The layers were separated and the aqueous layer was extracted with ethyi acetate (2x). The combined organics were washed sequentially with a saturated aqueous solution of sodium bicarbonate (2x) and brine (1x), then dried over sodium sulfate, filtered and concentrated under reduced pressure. The crude material was purified via HPLC to afford the title compound. MS (ESI+) (M+H) 420.2, HPLC rétention time: 2.04 min (Method A).

142

Th© compounds listed în Table 6 below were prepared using procedures analogous to those described above for the synthesîs of Example 72 using the appropriate starting materials which are available commercially or prepared using préparations well-known to those skilled in the art or prepared by a route described above.

Table 6

Example	Compound Name	^R²	Analytlcal Data
73	(R)-(1-(2-(1 -(4-Fluoro-1 H-pyrazol- 1 -yl)cyclopropyl)-3H-imidazo[4,5b]pyridin-5-yl)piperidin-3- yl) (pyrrolidïn-1 -yljmethanone	Y N—	MS (ES1+) (M+H) 424.2; HPLC rétention time: 1.99 min (Method A)
74	(R)-3-((5-(3-(Pyrrolidine-1carbonyî)pipertdin-1 -yl)-3 H· imidazo[4,5-b]pyridin-2yl)methyl)benzonitrile		MS (ES+) (M+H) 415.2; LCMS rétention time: 1.19 min (Method M)
75	(R)-4-((5-(3-(Pyrrolldlne-1 carbonyl)piperidin-1 -yl)-3Himtdazo(4,5-b]pyridin-2yl)methyl)benzonitrile	.N	MS (ES+) (M+H) 415.2; LCMS rétention time 1.20 min (Method M)
76	(fi)-1 -(1 -(5-(3-( Pyrrolid i ne-1 carbonyl)piperidin-1 -yl)-3H· imidazoÎ4₍5-b]pyridin-2yl)cyclopropyl)-1 H-pyrazole-4carbonitrile		MS (ESI+) (M+H) 431.2; HPLC rétention time: 1.99 min (Method A)

143

Example	Compound Name	^R²	Analytlcal Data
77	(fi)-(1 -(2-(1 -(4-Cyclopropyl-1 Hpyrazol-1 -yl)cyclopropyl)-3 H· imidazo[4,5-b]pyridin-5-yl)piperidin- 3-y I) (py rrolid in-1 -yl)methanone	X N'N	MS (ESI+) (M+H) 446.1; HPLC rétention time: 2.1377 min (Method A)
78	(fi)-Pyrrolidin-1 -yl( 1 -(2-(1 -(4(trifluoromethyl)-l W-pyrazol-1 yl)cyclopropyl)-3W-lmidazo[4,5b]pyridin-5-yl)plperidin-3yl)methanone	^F c X N-N	MS (ESI+) (M+H) 474.2; HPLC rétention time: 2.34 min (Method A)
79	(fi)-(1 -(2-(1 -(1H-Pyrazol-1 yl)cyclopropyl)-3H-imidazo[4,5b]pyridin-5-yl)piperidin-3yl) (py rroli din-1 -yl)methanone	Û	MS (ESI+) (M+H) 406.2; HPLC rétention time: 1.8063 min (Method A)
80	(fl)-(1 -(2-(1 -(IH-Pyrazol-1 yl)cyclobutyl)-3W-imidazo[4,5b]pyridin-5-yl)piperidin-3y I) (py rroli din-1 -yl)methanone	a •t	MS (ESI+) (M+H) 420.2; HPLC rétention time: 2.04 min (Method A)

Example 81 : 1 -(2-( 1 -f Pvridin-3-vl)cvclopropvlÎ-3H-imidazor4.5-b1pvridin-5vnpiperidin-3-vlÏÏPViTolidin-1-vnmethanone

Step 1: ffî>M(2-Amino-6-(3-(pyrrolidine-1-carbonyl)piperidin-1-yl)pyridin-3-yl)-1(pyridin-3-yl)cyclopropanecarboxamide

1-(Pyridin-3-yl)cyclopropanecarboxylic acid (30.7 mg, 0.188 mmol), (7^-(1-(5,6dÎaminopyridin-2-yl)plperidin-3-yl)(pyrrolidin-1-yl)methanone dihydrochloride

144

W (Intermediate 1) (75 mg, 0.19 mmol), and W-methylmorpholine (83 pL, 0.76 mmol) were combined and dissolved in A/,A/-dimethylformamide (1.0 mL). Benzotriazole-1-yl-oxytris-(dimethylamino)-phosphonium hexafluorophosphate (BOP) (122 mg, 0.207 mmol) was added and the reaction mixture was stirred at 50°C for 18h. The solvent was removed under reduced pressure and the residue was dissolved in dichloromethane. A saturated aqueous solution of sodium bicarbonate was added. The organics were collected and the aqueous layer was extracted with dichloromethane. The combined organics were washed with brine, dried over sodium sulfate, filtered and concentrated under reduced pressure. The residue was purified via flash chromatography (0-10% methanol in dichloromethane) to afford (R)-M-(2-amino-6-(3-(pyrrolÎdine-1 carbonyl)piperidin-1 -yl)pyridin-3-yI)-1 -(pyridin-3-yl)cyclopropanecarboxamide (69 mg, 84%). MS (AP+) (M+H) 435.2; LCMS rétention time 1.51 min (Method L).

Step 2: (R>(1-(2-( 1-(Pyridin-3-yl)cyclopropyl)-3H-imidazo[4,5-b]pyridin-5-yl)piperidin-3¹⁵ yl)(pyrrolidin-1-yl)methanone

To a solution of ('fî>W-(2-amino-6-(3-(pyrrolidine-1-carbonyl)piperidin-1-yl)pyridin-3-yl)1-(pyridin-3-yl)cyclopropanecarboxamide (35 mg, 0.081 mmol) in isobutanol (500 pL) was added sodium methoxide (18 mg, 0.335 mmol) in methanol (250 pL). The reaction mixture was shaken 110°C for 18h. The solvent was evaporated under a nitrogen stream. The residue was partitioned between water (0.5 mL) and ethyl acetate (1.5 mL x 3). The combined organics were dried over magnésium sulfate, filtered and concentrated under reduced pressure. The residue was purified via HPLC to afford (fî>(1-(2-(1-(pyridÎn-3-yl)cyclopropyl)-3H-imidazo[4,5-b]pyridin-5-yl)piperidin-3yl)(pyrrolidin-1-yl)methanone (7.2 mg). MS (ESI+) (M+H) 417.3; HPLC rétention time

1.59 min (Method A).

The compounds listed In Table 7 below were prepared using procedures analogous to those described above for the synthesis of compound of Example 81 using the appropriate starting materials which are available commercially or prepared using préparations well-known to those skilled in the art or prepared by a route described above.

145 • Table 7

Example	Compound Name	^R²	Analytical Data
82	(fî)-(1 -(2-(1 -(Pyridin-2yl)cyclopropyl)-3H-imidazo[4,5b]pyridin-5-yl)piperidîn-3yl)(pyrrolidin-1-yl)methanone		MS (ESI+) (M+H) 417.3; HPLC rétention time: 1.83 min (Method A)
83	(R)-(1 -(2-( 1 -Benzylcyclopropyl)3H-imidazo[4,5-b]pyridin-5· y!) piperidin-3-y I) (pyrrolidin-1 yljmethanone		MS (ESI+) (M+H) 430.3; HPLC rétention time: 2.36 min (Method A)
84	(fî)-(1-(2-(4-Cyclopropylthiazol-2yl)-3H-imîdazo[4,5-b]pyridin-5yl)piperidin-3-yl) (pyrrolidin-1 yljmethanone		MS (ESI+) (M+H) 423.1 ; HPLC rétention time: 2.42 min (Method A)
85	(R)-(1-(2-(3-Benzyloxetan-3-yl)3H-imidazo[4,5-b]pyridin-5y I Jpiperid in-3-yl) (py rro lidin-1 yljmethanone		MS (ESI+) (M+H) 446.1; HPLC rétention time: 1.8063 min (Method A)

146 • Example 86: fffHI -(2-(1 -(3-MethvlÎsoxazol-5-vl)cvcloproovl)-3H-imidazo|4,5-b1pvridin-

5-yl)plperidin-3-vD(pvrrolidin-1-vnmethanone

Step 1 : (77,l-A/-(2-Amino-6-(3-(pyrrolidine-1 -carbonyl)piperidin-1 -yI)pyridine-3-yl)-1 -(35 methy llsoxazo I- 5-yi) cy clop ropaneca rboxamide

1-(3-Methylisoxazol-5-yl)cyclopropanecarboxylic acid (40 mg, 0.24 mmol), (fl>(1-(5,6diaminopyridin-2-yl)piperidin-3-yl)(pyrrolidin-1-yl)methanone dihydrochioride (Intermediate 1) (86 mg, 0.24 mmol), and diisopropylethylamine (200 pL, 1.2 mmol) were combined and dissolved in ΛΖ,Ν-dimethylformamide (1.25 mL). O-Benzotriazole10 /V,W,N',N’,4etramethyluronium hexafluorophosphate (HBTU) (109 mg, 0.287 mmol) was added and the reaction mixture was stirred at 40°C for 18h. The solvent was removed under reduced pressure and the residue was dissolved in dichloromethane. A saturated aqueous solution of sodium bicarbonate was added. The organlcs were collected and the aqueous layer was extracted with dichloromethane. The combined organlcs were washed with brine, dried over sodium sulfate, filtered and concentrated under reduced pressure. The residue was purified via flash chromatography (0-12% methanol in dichloromethane) to afford (77)-/V-(2-amino-6-(3-(pyrrolidine-1-carbonyÎ)piperidin-1yl)pyridine-3-yl)-1-(3-methylisoxazol-5-yi)cyclopropanecarboxamide (56 mg, 53%). MS (ES+) (M+H) 439.2; LCMS rétention time 1.94 min (Method L).

Step 2: (fi>(1 -(2-(1 -(3-Methylisoxazoi-5-yl)cyclopropyl)-3H-imidazo[4,5-b]pyridin-5y I) piperidi n-3-y I) (py rroli din-1 -yijmethanone

To a solution of (fl>/V-(2-amino-6-(3-(pyrrolidine-1-carbonyl)piperidin-1-yi)pyridine-3-yl)1-(3-methyiisoxazol-5-yl)cyclopropanecarboxamide (94 mg, 0.21 mmol) ln isobutanol (2mL) was added sodium methoxide (25% in methanol, 98pL, 0.43 mmol) followed by methanol (0.9 mL). The reaction mixture was stirred at 110°C for 18 h. An additional portion of sodium methoxide (25% in methanol, 98 pL, 0.43 mmol) was added and the reaction mixture was stirred at 110°C for 3 h. The mixture was concentrated under reduced pressure. The residue was dissolved in dichloromethane, washed with a saturated aqueous solution of sodium bicarbonate, dried over sodium sulfate, filtered and concentrated under reduced pressure. The crude material was purified via HPLC

147 to afford ffî>(1 -(2-(1 -(3-methylisoxazol-5-yl)cyclopropyl)-3H-lmidazo[4,5-b]pyridin-5yl)piperidin-3-yl)(pyrrolidin-1-yl)methanone. MS (ESI+) (M+H) 421.2; HPLC rétention time 2.02 min (Method A).

The compounds listed ln Table 8 below were prepared using procedures analogous to those described above for the synthesis of Example 86 using the appropriate starting materials which are available commercially or prepared using préparations well-known to those skilled in the art or prepared by a route described above.

Table 8

Example	Compound Name	ITr²	Analytical Data
87	(R)-( 1 -(2-(1 -(5-Methylisoxazol-3yl)cyclopropyl)-3H-imidazo[4,5b]pyridin-5-yl)piperidin-3y I ) (pyrrolid in-1 -yl)methanone		MS (ESI+) (M+H) 421.2; HPLC rétention time: 2.1 min (Method A)
88	(R)-(1 -(2-(1 -(Pyridin-3yl)cyclobutyl)-3H-imidazo[4,5b]pyridin-5-yl)piperidin-3yl)(py rrolidi n-1 -yljmethanone	S'	MS (ESI+) (M+H) 431.2; HPLC rétention time: 1.99 min (Method B)

148 φ Examples 89 and 90: (fî)-(1-(2-(1-(3-Methvi-1 H-pyrazol-1-vl)cvclopropvl)-3Himidazof4,5-blpvridin-5-vl)p!peridin-3-vl)(pyrrolidin-1 -vllmethanone and ffl)-(1 -(2-(1 -(5rnethyl-1 H-pvrazol-1-vl)cvclopropvl)-3H-imÎdazor4,5-b]pvridin-5-vl)plperidin-3vl)(pvrrolidin-1 -vDmethanone

Step 1 : ('fl/-N-(2-Amino-6-(3-(pyrrolidine-1 -carbonyl)piperidin-1 -yl)pyridin-3-yl)-1 -(3methyl-1 H-pyrazol-1-yl)cyclopropanecarboxamide or (R)-M-(2-amino-6-(3-(pyrrolidine-1carbonyl)piperidin-1 -yl) pyridïn-3-yl)-1 -(5-methyl-1 H-pyrazol-1 yl)cyclopropanecarboxamide

Into a vial was added Intermediate 10 (60.0 mg, 0.361 mmol), (Ή>(1-(5,6diaminopyridin-2-yl)piperidin-3-yl)(pyrrolidin-1-yl)methanonedihydrochloride (Intermediate 1) (131 mg, 0.361 mmol), diisopropylethyl amine (314 pL, 1.80 mmol), O(7-Azabenzotriazol-1 -yl)-N,N,M',Λ/’,-tetramethyluronium hexafluorophosphate (HATU) (206 mg, 0.541 mmol), and A/,N-dimethylformamide (3.0 mL). The reaction mixture was stirred at room température for 2 h. The mixture was purified via flash chromatography (0-6% methanol In dichloromethane) to afford (fl>N-(2-annino-6-(3-(pyrrolidine-1carbonyl)piperidin-1 -yl)pyridin-3-yl)-1 -(3-methyl-1 H-pyrazol-1 yl)cyclopropanecarboxamide or (H)-M-(2-amino-6-(3-(pyrrolidine-1 -carbonyl)piperidin-1 yl)pyridin-3-yl)-1 -(5-methyl-1 H-pyrazol-1 -yl)cyclopropanecarboxamide (62 mg).

Step 2: -(2-(1 -(3-Methyl-1 H-pyrazol-1 -yl)cyclopropyl)-3H-imîdazo[4,5-b]pyridin-5yl)piperidin-3-yl) (py rrolidin-1 -yl)methanone or (fi>(1 -(2-(1 -(5-methyl-1 H-pyrazol-1 yl)cyclopropyl)-3H-imidazo[4,5-b]pyridin-5-yl)piperidin-3-yl)(pyrrolidin-1-yl)methanone Into a vial containing (H}-W-(2-amino-6-(3-(pyrrolidine-1-carbonyl)piperidin-1-yl)pyridin25 3-yl)-1-(3-methyl-1 H-pyrazol-1 -yl)cyclopropanecarboxamlde or (R)-N-(2-amîno-6-(3(py rrolidine-1 -carbonyl)piperidin-1 -y I) py ridin-3-y l)-1 -(5-methyl-1 H-pyrazol-1 yl)cyclopropanecarboxamlde (60 mg, 0.14 mmol) and sodium methoxide (25% wt in methanol, 118pL) was added Isobutanol (0.4 mL) and methanol (0.2 mL). The réaction mixture was heated to 110°C for 5 h. The solvent was removed under reduced pressure and the residue was purified via HPLC to afford (Ή>(1 -(2-(1 -(3-methyl-1 Hpyrazol-1-yl)cyclopropyl)-3H-Îmidazo[4,5-b]pyridin-5-yl)piperidin-3-yl)(pyrrolidin-1149 yl)methanone or -(2-(1-(5-methyl-1 H-pyrazol-1 -yl)cyclopropyl)-3H-lmidazo[4,5b]pyndin-5-yl)piperidin-3-yl)(pyrrolidin-1-yl)methanone (Example 89, starting from Intermediate 10): MS (ESI+) (M+H) 420.3; HPLC rétention time 1.85 min (Method A).

An anaiogous procedure was used for the synthesis of the other regioisomer, starting from 84.9 mg of Intermediate 11. Example 90: MS (ESI+) (M+H) 420.2; HPLC rétention time 1.99 min (Method A).

Example 91: ffl )-(1-(2-( 1-(Pvrimidin-5-vl)cvclODropvD-3H-imÎdazor4,5-b1pvridin-5vl)piperidin-3-vl)pvrrolldin-1-vQmethanone

To a solution of (H/'(1-(5,6-diaminopyridin-2-yl)piperidin-3-yl)(pyrrolidin-1-yl)methanone dihydrochloride (64 mg, 0.18 mmol) in methanol (0.5 mL) and acetic acid (160 pL, 2.9 mmol) was added triethylamine (100 pL, 0.72 mmol). A solution of ethyl 1-(pyrimidin-5yl)cyclopropanecarbimidate hydrochloride (40 mg, 0.18 mmol) in methanol (0.3 mL) was added. The reaction mixture was stirred at 70°C for 18 h. The solvent was removed under reduced pressure and the residue was dissolved In ethyl acetate. The organics were washed with a saturated aqueous solution of sodium bicarbonate, and then with water, dried over sodium sulfate, filtered and concentrated under reduced pressure. The residue was purified via HPLC. MS (ESI+) (M+H) 419.9; HPLC rétention time: 1.694 min (Method A).

Example 92: ffl)-(1-(2-(1-f2-MethoxvphenvlÎcvclopropvlÎ-3H-Îmidazor4.5-blpvridin-5vl)pÎperidin-3-vl)(pvrrolÎdîn-1-vnmethanone

To a solution of (7?>(1-(5,6-dÎamÎnopyridin-2-yl)plperidin-3-yl)(pyrrolidin-1-yl)methanone dihydrochloride (32 mg, 0.088 mmol) in éthanol (0.5 mL) was added triethylamine (50pL, 1.42 mmol) and acetic acid (82pL, 1.42 mmol). Then a solution of ethyl 1-(2150

methoxyphenyl)cyclopropanecarbimidate hydrochloride (50 mg, 0.09 mmol) ln éthanol (0.3 mL) was added and the reaction mixture was heated to 75°C for 13 h. The solvent was removed under reduced pressure and the residue was partitioned between ethyl acetate and a saturated aqueous solution of sodium bicarbonate. The organic layer 5 was washed with water, dried over sodium sulfate, filtered and concentrated under reduced pressure. The crude material was purified via HPLC to afford the titie compound. MS (ESI+) (M+H) 446.1; HPLC rétention time: 2.299 min (Method A).

The compounds listed in Table 9 below were prepared using procedures analogous to those described above for the synthesis of Example 92 using the appropriate starting 10 materials which are available commercially or prepared using préparations well-known to those skilled in the art or prepared by a route described above.

Table 9

Example	Compound Name	-B-R²	Analytical Data
93	(fi)-(1 -(2-(1 -(lsoxazo!-3yl)cyclopropyl)-3H-imidazo[4,5b]pyridin-5-y!)piperidin-3y!) (py rrolidin-1 -yl)methanone		MS (ESI+) (M+H) 407.3; HPLC rétention time: 1.99 min (Method A)
94	(fi)-(1-(2-(1-(4Methoxyphenyl)cyclopropyl)-3Himidazo[4,5-b]pyridin-5yljpiperi din-3-yl) (py rrolidin-1 yl)methanone		MS (ESI+) (M+H) 446.1; HPLC rétention time: 2.2687 min (Method A)

Example 95: (fî)-(1-(2-(2-Cvclopropvlpvrimldin-4-vl)-3ffimidazof4.5-b1pvridÎn-5vl)piperidin-3-vl)(pyrrolidin-1-v!)methanone

151

To a suspension of fH>(1-(5,6-diaminopyridin-2-yl)piperidin-3-y1)(pyrrolidin-1yljmethanone dihydrochloride (1.0 g, 2.76 mmol) and acetic acid (2.5 mL, 43.4 mmol) in éthanol (7 mL) was added triethylamine (1.5 mL, 10.8 mmol). A solution of ethyl 2cyclopropylpyrimidine-4-carbîmidate (530 mg, 2.77 mmol) in éthanol (3 mL) was added. The reaction mixture was heated at reflux for 30 min, then was cooled. The solvent was removed under reduced pressure and ethyl acetate (100 mL) was added to the residue followed by water (10 mL) and a saturated aqueous solution of ammonium chloride (50 mL). The layers were separated and the organics were washed with a saturated aqueous solution of sodium bicarbonate (75 mL) and brine (50 mL), dried over magnésium sulfate, fiitered and concentrated under reduced pressure. To the resulting residue was added dichloromethane (4 mL) and methyl tert-butyl ether (35 mL). The mixture was stirred at 40°C unit! it was homogeneous. The solution was cooled to room température and stirred for 18 h. The resulting solids were fiitered and rinsed with ether to afford the title compound. ’H NMR (400 MHz, CDCIa) δ 1.10-1.17 (m, 2H) 1.18-1.23 (m, 2H), 1.57-1.70 (m, 1H) 1.82-2.05 (m, 7H) 2.27-2.34 (m, 1H) 2.63-2.72(m,1H) 2.973.07(m, 1H) 3.14-3.22 (m, 1H) 3.43-3.54 (m, 3H) 3.58-3.67 (m, 1H) 4.37(d, 1H) 4.53(d, 1H) 6.76 (d,1 H) 7.95 (m, 2H) 8.67 (d, 1H) 10.46 (br, 1H). MS (ES+) (M+H) 418.5; LCMS rétention time 2.72 min (Method L).

The compounds listed in Table 10 below were prepared using procedures analogous to those described above for the synthesis of compound of Example 95 using the appropriate starting materials which are availabie commercîally or prepared using préparations well-known to those skilled in the art or prepared by a route described above.

Table 10

Example	Compound Name	-B-R²	Analytical Data
96	(R)-Ethyl 2-methoxy-6-(5-(3(pyrroiidine-1 -carbonyl)piperidin-1 yl)-3H-imldazo[4,5-b]pyridin-2yljisonîcotinate	4°	MS (ESI+) (M+H) 479.2; HPLC rétention time: 2.66 min (Method A)

152

Example	Compound Name	^R²	Analytlcal Data
97	(fi)-Py rrol idin-1 -yl(1 -(2-(1 -mtolylcyclopropyl)-3H-imidazo[4,5b]pyridin-5-yl)piperidin-3yl)methanone		Ή NMR (500 MHz, CDCh) δ 1.18-1.34 (m, 2H) 1.41 (d, 2H) 1.75-1.83 (m, 3H) 1.85-1.89 (m,2H) 1.92-1.96 (m, 2H) 2.39 (s, 3H) 2.56-2.67 (m, 1H) 2.87 (t, 1H) 3.04 (t, 1H) 3.41-3.50 (m, 3H) 3.74 (q, 1H) 4.20 (d, 1H) 4.30 (d, 1H) 6.61 (d, 1H) 7.157.19 (m, 1H) 7.287.34 (m, 3H) 7.74 (d, 1H) 8.53 (br s, 1H) MS (ES+) 430.3; LCMS rétention time: 2.29 min (Method L)
98	( fi)-( 1 -(2-(4-Cyclopropylpyridin-2yl)-3H-imidazo[4,5-b]pyridin-5yl)piperidin-3-yl)(pyrrolidin-1 yl)methanone	Λ	MS (ES+APCI) (M+H) 417.2; LCMS rétention time 3.021 min (Method Z1)
99	(fi)-(1-(2-(4-Cyclopropylpyrimidin2-yl)-3H-imidazo[4,5-b]pyridin-5yl)pipe ridin-3-yl) (py rro lidin-1 yl)methanone	N=\	¹H NMR (400 MHz, CDCh) δ 1.14-1.21 (m,2H), 1.24-1.30 (m, 3H), 1.62-1.68 (m, 1H), 1.80-1.94 (m, 3H), 1.94-2.15 (m, 4H), 2.59-2.73 (m, 1H), 3.00 (td, 1H), 3.16 (dd, 1H), 3.433.55 (m, 3H), 3.583.67 (m, 1H), 4.40 (br s, 1H), 4.51 (d, 1H), 6.73 (d, 1H), 7.10 (d, 1H), 7.93 (d, 1H), 8.61 (d, 1H), 10.15 (brs, 1H)

153

Exampte	Compound Name	-B-R²	Anaîytical Data
100	(fi)-/V-Methyl-5-(5-(3-(pyrrolidine-1carbonyl)piperidin-1 -yl)-3 Himidazo[4,5-b]pyridin-2-yl)pyridine3-sulfonamide	K.» hn' o \	1H NMR (400 MHz, DMSO-cfe) δ 1.23 (s, 1H), 1.53 (d, 1H), 1.65Ί.74 (m,2H), 1.75Ί.Θ3 (m, 2H), 1.85-1.94 (m, 3H), 2.60-2.72 (m, 2H), 2.94 (d, 2H), 3.263.30 (m, 3H), 3.433.58 (m, 2H), 4.35 (d, 1 H),4.42 (d,1H), 6.91 (d, 1H), 7.787.90 (m, 2H), 8.81 (s, 1H), 8.92 (brs, 1H), 9.47 (brs, 1H), 13.41 (brs, 1H)
			MS (ES+) (M+H) 470.1; LCMS rétention time 3.845 (Method I)
101	(fi)-(1-(2-(6-Cyclopropylpyrazin-2yl)-3H-imidazo[4,5-b]pyridin-5y l)pipe ridi π-3-yl) (py rrol idin-1 · yl)methanone	/=N K	MS (ESI+) (M+H) 418.2; HPLC rétention time 2.3 min (Method A).

154

Exampie 102: (fî)-(1-(2-(1 •(3-Methoxvphenvl)cvclopropvlÎ-3H-imidazof4,5-b1pvridÎn-5vl)piperidin-3-vD(pyrrolÎdin-1-vl)methanone

To a solution of (fî>(1-(5,6-diaminopyridin-2-yl)piperidin-3-yl)(pyrrolidin-1-yl)methanone dihydrochloride (60 mg, 0.17 mmol) in methanol (0.5 mL) and acetic acid (150pL, 2.7 mmol) was added triethylamine (94 pL, 0.67 mmol) and a solution of ethyl 1-(3methoxyphenyl)cyclopropanecarbimidate hydrochloride (40mg, 0.16 mmol) in methanol (0.3 mL). The reaction mixture was heated in a microwave for 45 min at 130°C. The solvent was removed under reduced pressure and the residue was partitioned between ethyl acetate and a saturated aqueous solution of sodium bicarbonate. The organic layer was washed with water, dried over sodium sulfate, filtered and concentrated under reduced pressure. The crude material was purified via HPLC to afford the title compound. MS (ESI+) (M+H) 446.3; HPLC rétention time 2.3 min (Method A).

Example 103: ffl)-Pvrrolidin-1 -vl(1 -(2-( 1 -(p-tolvl)cvclopropvl)-3H-Îmidazo!4.5-blpvridin5-vl)piperidin-3-vl)methanone

To a suspension of (R)-(1-(5,6-diaminopyridin-2-yl)piperidin-3-yl)(pyrrolidin-1yl)methanone dihydrochloride (220 mg, 0.60 mmol) in éthanol (2 mL) was added triethylamine (250 pL, 1.8 mmol), a solution of ethy! 1-(p-tolyl)cyclopropanecarbimidate hydrochloride (140 mg, 0.58 mmol) in éthanol (1 mL), and acetic acid (0.5 mL, 8.70 mmol). The reaction mixture was heated in a microwave for 45 min at 130°C. The solvent was removed under reduced pressure. Ethyl acetate (10 mL) was added to the residue and the solution was washed with aqueous sodium hydroxide (1 N, 5 mL). The aqueous layer was extracted with ethyl acetate (10 mL x 3). The combined organics were washed with brine, dried over magnésium sulfate, filtered and concentrated under reduced pressure. The residue was purified via HPLC to afford the title compound. MS (ESI+) (M+H) 430.2; HPLC rétention time 2.51 min (Method A).

155

Example 104: -(2-(1-(1-MethvMH-pvrazol-5-vncvclopropvn-3H-imidazof4.5blpvridÎn-5-vnpÎperidin-3-vlÎ(pvrrolidin-1-vnmethanone

To a solution of 2-(1-methyl-1 H-pyrazol-5-yl)acetonitrile (14.5 mg, 0.10 mmol) In éthanol (150 pL, 2.6 mmol) was added acetyl chloride (110 pL, 1.5 mmol). The réaction mixture was stirred at room température for 16 h. The solvent was removed under a stream of nitrogen. Ethanol (0.5 mL) was added to the residue followed by a solution of (77)-(1(5,6-diaminopyridin-2-yl)piperidin-3-yl)(pyrrolidin-1-yl)methanone dihydrochloride (36 mg, 0.01 mmol) and triethylamine (70 pL, 0.50 mmol) In éthanol (1 mL). Acetic acid (100 pL,

1.75 mmol) was added and the réaction mixture was heated in the microwave for 45 min at 130°C. The solvent was removed under a stream of nitrogen. Ethyl acetate (10 mL) was added to the residue. The solution was washed with aqueous sodium hydroxide (1 N, 3 mL), washed with water (3 mL), washed with brine (3 mL), dried over magnésium sulfate, filtered and concentrated under reduced pressure. The crude material was purified via HPLC to afford the title compound.. MS (ESI+) (M+H) 420.3; HPLC rétention time 1.88 min (Method B).

Example 105: (W-Ethvl 2-ethvl-6-(5-(3-(pvrrolidine-1-carbonvQpiperidin-1-vO-3Himidazor4.5-blpvridin-2-v0isonicotinate

To a solution (H)-(1-(5,6-dÎamÎnopyridÎn-2-yl)piperidin-3-yl)(pyrrolidin-1-yl)methanone dihydrochloride (116 mg, 0.595 mmol) in anhydrous éthanol (5.0 mL) was added acetic acid (0.293 mL, 5.12 mmol) followed by triethylamine (0.179 mL, 1.28 mmol). Ethyl 2(ethoxy(imino)methyl)-6-ethylisonicotinate hydrochloride (80 mg, 0.32 mmol) was added

156 and th© reaction mixture was heated to 110°C for 30 min. The reaction mixture was cooled to room température and th© solvent was removed under reduced pressure.

The résidu© was partitioned between a saturated aqueous solution of ammonium chloride (50 mL) and ethyl acetate (50 mL). The pH of the aqueous layer was adjusted to 3 using an aqueous solution of hydrochloric acid (1N). Th© aqueous layer was extracted with ethyl acetate (50 mL x 2). The combined organic layers were washed with brine, dried over sodium sulfate, filtered and concentrated under reduced pressure. The crud© material was purified via HPLC to afford the title compound. MS (ESI+) (M+H) 477.2; HPLC rétention time 2.7 min (Method A).

Example 106: ff?>Pvrrolidin-1 -vl(1 -(2-(1 -(4-(trifluoromethvl)phenvl)cvclopropyl)-3H· lmidazor4,5-b1pvridin-5-vl)piperidin-3-vl)methanone

The title compound was prepared by a method analogous to the one used for Example 92, but methanol was used as the solvent and the reaction mixture was heated to 70°C. ’H NMR (500 MHz, CD₃OD) δ 1.60-1.82 (m, 6H) 1.87-2.05 (m, 6H) 2.72-2.81 (m, 1H) 2.91-2,99 (m, 2H) 3.43 (t, 2H) 3.53 (dt, 1H) 3.68-3.75 (m, 1H) 4.26 (d, 1H) 4.53 (d, 1H) 6.78 (d, 1H) 7.52 (d, 2H) 7.65 (d, 3H).

Example 107: (R)-(1-(2-(6-Cvclopropvlpyraztn-2-vD-3H-innidazor4.5-b1pvridin-5vl)piperidin-3 -vl)(morpholino)methanone o,

The title compound was prepared by a method analogous to th© one used for Example

95, but using (fî)-(1-(5,6-diaminopyridin-2-yl)piperidin-3-yl)(morpholino)m©thanone dihydrochlorid© (synthesized by hyrdrogenetion analogous to the one used for

Intermediate 1, starting from Intermediate 40) and Intermediate 30. ’H NMR (300 MHz,

DMSO-cfe) δ 1.06 (dd, 2H), 1.20-1.33 (m, 3H), 1.54-1.74 (m, 3H), 1.80-1.93 (m, 1H),

157 ® 2.19-2.33 (m, 2H), 2.79 (br s, 1 H), 2.87-3.09 (m, 3H), 3.55 (m, 5H), 4.30-4.53 (m, 2H),

6.88 (d, 1H), 7.86 (d, 1 H), 8.61 (s, 1H), 9.06 (s, 1H), 13.05 (s, 1H)

Example 108: (/7)-(1-(8-(1-(4-Chloro-1 /7-pvrazol-1-vl)cvcloDropvl)-9/7-purin-2vl)piperidin-3-vl)(pvrrolÎdÎn-1-vl)methanone

To a solution of (/7)-(1-(4,5-diaminopyrimidin-2-yl)piperidin-3-yl)(pyrrolidin-1yl)methanone dihydrochloride (320 mg, 0.881 mmol) and ethyl 1-(4-chloro-1H-pyrazol1-yl)cyclopropanecarbimidate (227 mg, 1.06 mmol) in éthanol (2 mL) was added glacial acetic acid (0.81 mL, 14 mmol) and triethylamine (0.50 mL, 3.5 mmol). The solution was heated to 110°C for 2 h. The solvent was removed under reduced pressure and the residue was dissolved in dichloromethane. The organics were washed with a saturated aqueous solution of ammonium chloride. The aqueous layer was extracted with dichloromethane. The combined organics were dried over sodium sulfate, filtered and concentrated under reduced pressure. The crude material was purified by flash chromatography (0-10% methanol in ethyl acetate) to give the product as a red solid. The solid was further purified via flash chromatography (0-20% acetone in dichloromethane), followed by passing through a plug of basic alumina (80-100% ethyl acetate in heptanes) to give the title compound as a pink solid (75 mg, 19% yieid). NMR (500 MHz, CDCI₃) δ 1.46-1.55 (m, 1H), 1.75-2.00 (m, 11 H), 2.51-2.57 (m, 1H),

2.85-2.90 (m, 1 H), 3.04 (dd, 1 H), 3.39-3.50 (m, 3H), 3.58-3.63 (m, 1 H), 4.72-4.83 (m,

2H), 7.56 (s, 1 H), 7.63 (s, 1 H), 8.57 (br s, 1 H), 9.83 (br s, 1 H). MS (AP+) (M+H) 441.2; LCMS rétention time 2.63 min (Method L).

Example 109-A: (ff)-(1-(2-(1-(4-Chloro-1 H-pvrazol-1-vl)cvclopropvl)-3WmÎdazof4.5blpvridin-5-vl)plperidin-3-vl)(pvrrolidin-1-vl)methanone

158

To a solution of (R)-(1-(5,6-diaminopyridin-2-yl)piperidin-3-yl)(pyrrolidin-1-yl)methanone dihydrochloride (5.5 g, 15.2 mmol) and acetic acid (17.4 mL, 305 mmol) in éthanol (20 mL) was added a solution of ethyl 1-(4-chloro-1H-pyrazol-1-yl)cyclopropanecarbimidate 5 (3.25 g, 15.2 mmol) in éthanol (30 mL) followed by triethylamine (12.7 mL, 91.2 mmol).

The resulting mixture was purged with nitrogen. The reaction mixture was stirred for 18 h at 100°C. The mixture was cooled to room température and the solvent was removed under reduced pressure. The residue was partitioned between a saturated aqueous solution of ammonium chloride (50 mL) and dichloromethane (50 mL). The aqueous 10 layer was extracted with dichloromethane (50 mL). The combined organics were washed with a saturated aqueous solution of sodium bicarbonate and the resulting aqueous layer was extracted with dichloromethane (50 mL). The organics were combined, washed with brine, dried over sodium sulfate, filtered and concentrated under reduced pressure. The crude material was purified via flash chromatography (015 5% methanol In dichloromethane) to afford (/7)-(1-(2-(1-(4-ch1oro-1 H-pyrazol-1yl)cyclopropyl)-3H-imidazo[4,5-b]pyridin-5-yl)piperidin-3-yl)(pyrrolidin-1-yl)methanone (6.33 g, 94%). ’H NMR (500 MHz, CDCI₃) δ 1.55-1.70 (m, 1H), 1.72-2.05 (m, 11H), 2.60-2.70 (m, 1 H), 2.90-2.95 (m, 1H), 3.03-3.10 (m, 1H), 3.42-3.50 (m, 3H), 3.56-3.62 (m, 1H), 4.20-4.25 (m, 1H), 4.38-4.44 (m, 1H), 6.62-6.68 (m, 1 H). 7.59 (s, 1H), 7.63 (s, 20 1H), 7.66-7.72 (m, 1H); MS (ES+)(M+H) 440; UPLC rétention time 0.47 min (Method

N).

Alternative préparation for (R)-(1-(2-(1-(4-chloro-1 H-pyrazol-1-y1)cydopropy1)-3Hlmidazo[4,5-b]pyridin-5-yl)piperidin-3-yl)(pyrrolidin-1-yl)methanone

Step 1 : (R)-1 -(4-Chloro-1 H-pyrazol-1 -yl)-/V-(3-nitro-6-(3-(pyrrolidine-1 · carbonyl)piperidin-1 -yl)pyrîdln-2-yl)cyclopropanecarboxamide

159

W Into a 1 -L Atlas jacketed reactor were added 1 -(4-chloro-1 H-pyrazol-1 yljcyclopropanecarboxylic acid (49.08 g, 263.02 mmol), (R)-(1-(6-amino-5-nitropyridin-2yl)piperidin-3-yl)(pyrrolidin-1-yl)methanone (70 g, 219.19 mmol), 4dimethylaminopyridine (5.41 g, 43.84 mmol), toluene (600 mL) and dîisopropylethylamine (95.56 mL, 547.96 mmol). The reaction mixture was heated to 45 °C and stirred for 10 min untii most of the solids were dissolved. 1-Propanephosphonic acid cyclic anhydride (195.70 mL, 328.78 mmol, 50% solution in ethyl acetate) was added and the température was Increased to 110 °C. The réaction mixture was stirred for 19 h before adding more 1-propanephosphonic acid cyclic anhydride solution (25 mL). The mixture was stirred at 110 °C for 5 h. The mixture was concentrated to a low volume via distillation with jacket température at 80 °C. A mixture of ethanohwater (1:1, 1000 mL) was added to keep the température around 70 °C. Once the addition was complété, the mixture was cooled to 15 °C over a period of 2 h and left granulating for 18 h. The solids were filtered and rinsed with a mixture of ethanolwater (1:1, 750 mL) and dried in a vacuum oven at 40 °C with a nitrogen bleed for 18 h to afford (R)-1-(4chloro-1 H-pyrazol-1 -yl)-M-(3-nitro-6-(3-(pyrrolidine-1 -carbonyl)piperidin-1 -yl)py ridin-2yl)cyclopropanecarboxamlde (72 g). ’H NMR (500 MHz, CDCb) δ 1.50-1.66 (m, 4H), 1.82-2.05 (m, 11H), 2.53-2.61 (m, 1 H), 3.00-3.20 (m, 2H), 3.34-3.42 (m, 1H), 3.49 (t, 2H), 6.32 (d, 1H), 7.61 (s, 1H), 7.68 (s, 1H), 8.21 (d, 1 H), 11.01 (s, 1H); MS (ES+)(M+H)

488; HPLC rétention time: 7.520 min (Method; Column: Halo C18, 4.6 x 150 mm,

2.7pm; Mobile Phase B: 0.1 M phosphoric acid (H3PO4) In water; Mobile Phase D: acetonitrile; Gradient: 80%-10% Mobile Phase B untii 7.00 min; hold untii 10.00 min; rapid ramp of 80% Mobile Phase B untii 10.1 min; equilibrate untii 12.1 min; Flow: 0.8 mL/min; Column Température: 30 °C; DAD1A, Sig=254 nm).

Step 2: (R)-(1-(2-(1-(4-Chloro-1 H-pyrazol-1-yl)cyclopropyl)-3H-imidazo[4,5-b]pyridin-5yl)piperidin-3-yl)(pyrrolidin-1-yl)methanone

Into a flask was added (R)-1-(4-chloro-1 H-pyrazol-1-yl)-M-(3-nitro-6-(3-(pyrrolidine-1carbonyl)piperidin-1-yl)pyridin-2-yl)cyclopropanecarboxamide (107 g, 219.29 mmol) and acetic acid (750 mL, 6.74 mol). The mixture was stirred for 5 min. Zinc powder (71.70 g, 1.10 mol) was added. An exotherm to 79 °C was observed over a period of 30 sec. The reaction mixture was warmed to 100 °C and stirred for 2 h. The mixture was cooled to 50 °C and water (750 mL) was added. The mixture was stirred for 10 min and cooled to 10 °C. Ethyl acetate (500 mL) was added and the mixture was stirred for 20 min and

160 filtered through Celite rinslng with ethyl acetate (300 mL). The filtrate was cooled to 10 °C and aqueous ammonium hydroxide (936.92 mL, 6.74 mol) was added dropwise over a period of 20 min. The layers were separated and the organics were washed with a mixture of water:brine (1:1,500 mL), and concentrated under reduced pressure. Ethyl acetate (200 mL) was added and the mixture was concentrated under reduced pressure followed by the addition of acetonîtrile (500 mL). The mixture was concentrated to dryness at 45 °C to afford (fî)-(1-(2-(1-(4-chloro-1H-pyrazoH-y1)cyclopropyl)-3Himidazo[4,5-b]pyridin-5-yl)piperidin-3-yl)(pyrrolidin-1-yl)methanone (100 g) as an amorphous material. ’H NMR (500 MHz, CDCl₃) δ 1.55-1.70 (m, 1H), 1.72-2.05 (m, 11 H), 2.60-2.70 (m, 1H), 2.90-2.95 (m, 1H), 3.03-3.10 (m, 1H), 3.42-3.50 (m, 3H), 3.56-

3.62 (m, 1H), 4.20-4.25 (m, 1H), 4.38-4.44 (m, 1H), 6.62-6.68 (m, 1H), 7.59 (s, 1H),

7.63 (s, 1H), 7.66-7.72 (m, 1H); MS (ES+)(M+H) 440; HPLC rétention time: 4.450 min (Method: Same as for Step 1).

Example 109-B: (fî)-(1-(2-(1-(4-Chloro-1H-pvrazol-1-vl)cvclopropvlÎ-3H-imidazor4.5blpvridin-5-vl)piperidin-3-vl)(pvrrolidin-1-vl)methanone methanesulfonate

To a solution of (A)-(1-(2-(1-(4-chloro-1H-pyrazol-1-yl)cyclopropyl)-3H-Îmidazo[4,5b]pyridin-5-yl)piperidin-3-yl)(pyrrolidin-1-yl)methanone (Example 109-A, 56.49 g, 128.4 mmol) in acetonîtrile (130 mL) was added methanesulfonîc acid (8.33 mL, 128 mmol). The mixture was stirred for 18 h at room température. The mixture was filtered and the solids were rinsed with acetonîtrile. The solids were collected and dried under high vacuum for 1 h to afford (A)-(1-(2-(1-(4-chloro-1H-pyrazol-1-yl)cyclopropyl)-3Himidazo[4,5-b]pyridin-5-yl)piperidin-3-yl)(pyrrolidin-1-yl)methanone methanesulfonate (62.5 g, 90%). ’H NMR (400 MHz, CDCl₃) δ 1.65-1.75 (m, 1H), 1.84-2.13 (m, 11H), 2.75-2.85 (m, 1H), 2.87 (s, 3H), 3.28-3.35 (m, 1H), 3.39-3.57 (m, 4H), 3.65-3.72 (m, 1H), 4.02-4.09 (m, 1H), 4.11-4.17 (m, 1H), 6.73-6.82 (m, 1H), 7.55 (s, 1 H), 7.76 (s, 1H), 8.13 (d, 1 H); MS (ES+)(M+H) 440; UPLC rétention time 0.47 min (Method N), mp = 212161

214°C. Anal. Calculated for C22H26CIN7O · CH4O3S: C, 51.53; H, 5.64; N, 18.29; Cl,

6.61; S, 5.98. Found: C, 51.28; H, 5.65; N, 18.19; Cl, 6.56; S, 5.96.

Alternative préparation for (R)-(1-(2-(1-(4-chloro-1 H-pyrazol-1-yl)cyclopropyl)-3H5 imidazo[4,5-b]pyridin-5-yl)piperidin-3-yl)(pyrrolidin-1 -yljmethanone methanesulfonate To (R)-(1-(2-(1-(4-chloro-1 H-pyrazol-1-yl)cyclopropyl)-3H-imidazo[4,5-blpyridin-5yl)piperidin-3-yl)(pyrrolidin-1 -yljmethanone (Example 109-A, 100 g), prepared by the alternative method described in Example 109-A (Step 1 and Step 2), was added acetonitrile (500 mL). The mixture was stirred for 5 min at room température before adding methanesulfonic acid (14.38 mL, 219.29 mmol) over a period of 15 sec. The mixture was stirred for 2 h while cooling back to 19 °C. The solids were filtered and washed with acetonitrile (250 mL) and dried under nitrogen for 18 h to afford the title compound (86 g, 73% over two steps from Step 2, alternative method for Example 109A) as a white solid. HPLC rétention time: 4.451 min (Method: Same as for Step 1 in the alternative method described for Example 109-A). Chiral HPLC rétention time: 3.837 min (Method: Column: OJ-H 4.6x250mm, 5gm; S10|(S): acetonitrile + 0.1% isopropylamine, 150 bar, 4 mL/min, 40°C, 0-5.5 min: 5-45% S, 5.5-7.5 min: 45% S, 7.51-8 min: 5% S). Ή NMR (400 MHz, DMSO-d_e) δ 1.35-2.03 (m, 12H), 2.32 (s, 3H), 2.53-2.65 (m, 1H), 2.91-3.13 (m, 2H), 3.20-3.35 (m, 2H), 3.35-3.55 (m, 2H), 4.20-4.36 (m, 2H), 7.05 (d, 1 H), 7.73 (s, 1 H), 7.83 (d, 1 H), 8.30 (s, 1 H).

Alternative préparation for (fî)-(1 -(2-(1 -(4-chloro-1 H-pyrazol-1 -yl)cyclopropyl)-3Himidazo[4,5-b]pyridin-5-yl)piperidin-3-yl)(pyrrolidin-1-yl)methanone methanesulfonate Step 1: (fî)-fert-Butyl 2-amino-6-(3-(pyrrolidine-1-carbonyl)piperidin-1-yl)pyridin-3ylcarbamate

A nitrogen-purged reaction vessel was charged sequentially with 5% palladium-oncarbon (0.208 kg, 0.10 mol), (fî)-(1-(6-amino-5-nitropyridin-2-yl)piperidin-3-yl)(pyrrolidin1-yl)methanone (1.3 kg, 4.1 mol), ethyl acetate (14 L), di-fert-butyl dicarbonate (0.906 kg, 4.15 mol), and triethylamine (0.824 kg, 8.14 mol). An additional charge of ethyl

162 acetate (1.3 L) was added to ensure ail residues were rinsed Into the reaction vessel.

The vessel was purged and pressurized with nitrogen, then was purged and pressurized with hydrogen to 50 psig. The reaction was heated to 40°C and was held at that température for 6 h. The mixture was then cooled to 20°C and the vessel was purged with nitrogen. The presence of (fl)-tert-butyl 2-amino-6-(3-(pyrrolidine-1 carbonyl)piperidin-1-yl)pyridin-3-ylcarbamate was confirmed by HPLC analysis of the mixture [HPLC rétention time: 5.25 min (Column: Halo C18, 4.6 x 150 mm, 2,7pm; Mobile Phase A: acetonitrile, Mobile Phase B: 0.05% methanesulfonic acid in water; Linear Gradient: 5:95 A:B to 95:5 A:B over 9 min, then held for 1 min; Flow: 1.0 mL/min; UV détection at 210, 226, and 254 nM)]. The mixture was fiitered and was rinsed with ethyl acetate (6.5 L). The filtrate was transferred to another réaction vessel, rinsing with ethyl acetate (1 L). To quench excess di-tert-butyl dicarbonate, /V,W-diethylamine (59.5 g, 0.81 mol) was added while maintaining a température of 20°C; the reaction mixture was held for 30 min at that température. Three batches of material on this scale were combined and were used in the next step without further purification.

Step 2: (fi)-(1 -(2-(1 -(4-Chloro-1 H-pyrazol-1 -yl)cyclopropyl)-3H-imidazo[4,5-b]pyridin-5yl) pipe ridin-3-y I) (py rrolidi n-1 -yljmethanone methanesulfonate ci

A solution of (fl)-tert-butyl 2-amino-6-(3-(pyrrolidine-1-carbonyl)pÎperidin-1-yl)pyridin-3’ ylcarbamate in ethyl acetate from the combined three batches from Step 1 (65 L total volume) was distilled under reduced pressure at 40°C until the residual volume was approxlmately 8 L. An additional portion of ethyl acetate (48 L) was added, and the resulting solution was distilled under reduced pressure at 40°C until the residual volume was approxlmately 8 L. Ethyl acetate (33 L), 1-(4-chloro-1 H-pyrazol-1yljcyclopropanecarboxylic acid (2.28 kg, 12.2 mol), and /V./V-diisopropylethylamine (4.74 kg, 36.7 mol) were added sequentlally. To the resulting mixture at 20°C was added a solution of 1-propanephosphonic acid cyclic anhydride in ethyl acetate (50% solution, 14.0 kg, 22.0 mol; plus a 2.0-L ethyl acetate rinse). The resulting mixture was heated to 40°C and was held at that température for 8 h. The presence of (fl)-tert-butyl 2-(1-(4

163 chloro-1 H-pyrazol-1 -yl)cyclopropanecarboxamido)-6-(3-(pyrrolidine-1 carbonyl)piperidin-1-yl)pyridin-3-ylcarbamate was confirmed by HPLC analysis of the mixture [HPLC rétention time: 8.53 min (Column: Halo C18,4.6 x 150 mm, 2.7pm; Mobile Phase A: acetonitrile, Mobile Phase B: 0.05% methanesulfonic acid in water; Linear Gradient: 5:95 A:B to 95:5 A:B over 9 min, then held for 1 min; Flow: 1.0 mL/min; UV détection at 210,226, and 254 nM)]. The mixture was then partitioned between ethyl acetate (38 L) and a 10% aqueous solution of citric acid (2 x 50 L). The organic layer was then washed sequentially with a 10% aqueous solution of potassium carbonate (31 L) and with water (24 L). The organic layer was then distilled under reduced pressure at 40°C until the remaining volume was approximateiy 8 L. Acetonitrile (49 L) was added and the solution was distilled under reduced pressure at 40°C until the remaining volume was approximateiy 26 L. Methanesulfonic acid (1.41 kg, 14.7 mol) was added via addition funnel, followed by an acetonitrile rinse (0.5 L). The reaction mixture was heated to 70°C and was held at that température for 12 h, then was cooled to 20°C over a period of 2 h. The resuiting suspension was filtered, rinsing with acetonitrile (29 L). The solids were dried under a nitrogen flow and then ln a vacuum oven (40°C) to afford (fi)-(1-(2-(1-(4-chloro-1 H-pyrazol-1-yl)cyclopropyl)-3H· lmidazo[4,5-b]pyridin-5-yl)piperidin-3-yl)(pyrrolidin-1-yl)methanone (3.1 kg, 47% over two steps). HPLC rétention time: 5.85 min (Column: Halo C18, 4.6 x 150 mm, 2.7pm; Mobile Phase A: acetonitrile, Mobile Phase B: 0.05% methanesulfonic acid in water; Linear Gradient: 5:95 A:B to 95:5 A:B over 12 min, then held for 2 min; Flow: 1.0 mL/min; UV détection at 210 nM). Chiral HPLC rétention time: 4.30 min; Column: AS-H 4.6 x 150 mm, 5pm; Mobile Phase A: supercritical carbon dioxide, Mobile Phase B: 0.1% Isopropylamine in methanol; Gradient: 5%B to 45%B over 6 min, then held at 45%B for 2 min; Flow: 4.0 mL/min; Column température 40°C; UV détection at 230 nM).

Exampie 109-C: (/7)-(1-(2-(1-(4-Chloro-1/-Apvrazol-1-vDcvcloDroDvl)-3/-Aimidazo['4.5biPvridin-5-vl)piperidin-3-vl)(pvrrolidin-1-vlÎmethanone hydrochloride

Cl

(fi)-(1 -(2-(1 -(4-Chloro-1 H-pyrazol-1 -yl)cyclopropy1)-3H-imidazo[4,5-b]pyridin-5yl)piperidin-3-yl)(pyrrolidin-1-yl)methanone (140 mg, Example 109-A) was dissolved in

164

0.5mL acetone at 22°C. A one molar équivalent of concentrated (36% in water) HCl was added, drop wise, to the rapidly stirred solution. The sample immediately tumed cloudy. Sample was warmed to 40'C and an additional acetone (0.5 mL) was added. After about 30 minutes, the sample was cooied (-1 ’C/min) to 22°C. White, opaque solids formed. The sample was vacuum filtered and the solids were collected/dried at room température (~22°C) to afford (fî)-(1 -(2-(1 -(4-Chloro-1 H-pyrazol-1 -yljcyclopropyl)3H-imidazo[4,5-b]pyridin-5-yl)piperidin-3-yl)(pyrrolidin-1-yl)methanone hydrochloride.

¹H NMR (400 MHz, DMSO-cfe) δ 1.43-2.01 (m, 12H), 2.60 (t, 1H), 2.92-3.10 (m, 2H), 3.29 (t, 2H), 3.33-3.46 (m, 1H), 3.46-3.56 (m, 1H), 4.26 (d, 1H), 4.32 (d, 1H), 7.04 (d, 1H), 7.72 (s, 1H), 7.82 (d, 1H), 8.32 (s, 1H).

Elemental analysis and Karl Fisher confirmed the resulting crystalline form to contain 4.4% water.

Anal. Calculated for C^HmCINtO · HCl · H₂O: C, 53.4; H, 5.9; N, 19.8; Cl, 14.3. Found: C, 53.3; H, 6.0; N, 19.2; Cl, 14.8.

Example 110: /HFMethvl 2-(3-(5-(3-(pvrrolîdine-1 -carbonvl)piperidin-1 -vl)-3Himidazof4,5-blpvridin-2-vl)phenvl)acetate

Into a vial was added methyl 2-(3-formylphenyl)acetate (98 mg, 0.55 mmol), (/7)-(1-(6amino-5-nitropyridin-2-yl)piperidin-3-yl)(pyiTolÎdîn-1-yljmethanone (Intermediate 1, Step 3) (140 mg, 0.45 mmol) and éthanol (1.5 mL). Then sodium dithionate (388 mg, 2.23 mmol) and water (0.5 mL) were added to the mixture. The vial was sealed. The reaction mixture was heated to 110°C for 18 h. The solution was cooied to room température. Triethylamine (0.15 mL, 1.1 mmol) was added and the solution was stirreed at 100°C for 18 h. A sample (0.8 mL) of the solution was removed and concentrated to dryness, and the resulting residue was purified via HPLC to afford the title compound. MS (ESI+)(M+H) 448.2; HPLC rétention time 2.28 min (Method A).

Example 111 : fl7F(1-(2-(6-(3-Hvdroxvoxetan-3-vl)pvridin-2-vl)-3H-imidazof4.5-blpvridin 5-vl)piperidin-3-vl)(pvrrolidin-1-vl)methanone

165

Into a flask was added 6-(3-hydroxyoxetan-3-yl)plcolinaldehyde (54 mg, 0.30 mmol), (fî>(1-(6-amino-5-nÎtropyridÎn-2-y!)piperidin-3-yl)(pyrrolÎdin-1-y!)methanone (Intermediate 1, Step 3) (76 mg, 0.24 mmol) and éthanol (1.2 mL). Then sodium dithionate (0.20 g, 1.2 mmol), triethylamine (82 pL, 0.59 mmol) and water (1.0 mL) were added. The solution was heated to 110°C for 18h. The mixture was cooled to room température and diluted with ethyl acetate. The mixture was washed with water, washed with a saturated aqueous solution of ammonium chloride, washed with a saturated aqueous solution of sodium bicarbonate and washed with brine. The organlcs 10 were dried over magnésium sulfate, filtered, and concentrated under reduced pressure.

The residue was dried under high vacuum to give a green oil which was purified via HPLC to afford the title compound. MS (ESI+) (M+H) 448.9; HPLC rétention time 1.89 min (Method A).

Example 112: ff?)-Pvrrolidin-1-vl(1-(2-(6-(trifluoromethvnpvridin-2-vl)-3Wmldazof4,515 bipvridin-5-vnpiperidin-3-vDmethanone

Into a tube was added 6-(trifluoromethyl)plcolinaldehyde (150 mg, 0.47 mmol), (fi>(1(6-amîno-5-nitropyridin-2-yl)piperidin-3-yl)(pyrrolidin-1 -yljmethanone (Intermediate 1, Step 3) (82.2 mg, 0.47 mmol), sodium dithlonite (310.7 mg, 1.78 mmol), éthanol (10 mL) 20 and water (1.5 mL). The vessei was sealed and the reaction mixture was stirred at

110°C for 16 h. The solvent was evaporated under reduced pressure and water was added to the resulting residue. The mixture was extracted with ethyl acetate, dried over sodium sulfate and concentrated under reduced pressure. The crude material was purified via préparative TLC to afford the title compound (50 mg, 29%) as a yellow solid.

MS (ES+APCI) (M+H) 445.2; LCMS rétention time 2.657 min (Method G).

The compounds listed in Table 11 below were prepared using procedures analogous to those described above for the synthesis of Example 112 using the appropriate starting 166 materiais which are available commercially or prepared using préparations well-known to those skilled ln the art or prepared by a route described above.

Table 11

Example	Compound Name	^R²	Analytical Data
113	(R)-(1-(2-(5-Fluoropyridin-3-yl)-3H· imidazo[4,5-b]pyridin-5-yl)piperidin- 3-yl) (pyrrolidin-1 -yl)methanone	-Q F	¹H NMR (400 MHz, DMSO-cfe) δ 1.211.28 (m,3H), 1.501.60 (m, 1H), 1.641.74 (m, 2H), 1.751.83 (m,2H), 1.841.96 (m, 2H), 2.592.66 (m, 1 H), 2.893.02 (m, 2H), 3.413.55 (m, 2H), 4.35 (d, 1H), 4.42 (d, 1H), 6.90 (d, 1H), 7.86 (d, 1H), 8.28 (d, 1H), 8.63 (d, 1H), 9.17 (s, 1H), 13.26 (s, 1H)MS (ES+APCI) (M+H) 395.1; LCMS rétention time: 2.861 min (Method C1)

167

Example	Compound Name	-B-R²	Analytical Data
114	(fi)-3-(5-(3-(Pyrrolidine-1 carbonyl)piperidin-1 -yl)-3Hlmidazo[4,5-b]pyridin-2yljbenzenesulfonamide	H₂N-/£⁼⁰0	’H NMR (300 MHz, DMSO-dg) δ 1.481.62 (m, 1H), 1.631.74 (m, 2H), 1.741.Θ3 (m, 2H), 1.Θ41.94 (m, 3H), 2.62 (brs, 1H), 2.84-3.00 (m, 2H), 3.17 (d, 1H), 3.42-3.56 (m, 2H), 4.28-4.47 (m, 2H), 6.87 (d, 1H), 7.42-7.50 (m, 2H), 7.64-7.76 (m, 1H), 7.80-7.87 (m, 2H), Θ.26 (d, 1 H), 8.65 (s, 1H), 13.20 (s, 1H) MS (ES+APCI) (ΜΗ) 453.1; LCMS rétention time: 3.766 min (Method D

168

Example	Compound Name	-B*R^Z	Analytical Data
115	(R)-(1 -(2-(6-(2-Hydroxypropan-2yl)pyridin-2-yl)-3H-imidazo[4,5b]pyridin-5-yl)piperidin-3- yl) (py rrol idin-1 -yl)methanone	Ή. HO	’H NMR (400 MHz, CDCI₃) Ô 1.26-1.29 (m, 2H), 1.63 (s, 6H), 1.76-2.06 (m, 6H), 2.69 (t, 1H), 2.95-3.06 (m, 1H), 3.12-3.20 (m, 1H), 3.43-3.55 (m, 3H), 3.58-3.68 (m, 1H), 4.33 (d, 1 H), 4.54 (d, 1H), 6.72 (d, 1 H), 7.45 (d, 1H), 7.79-7.93 (m, 2H), 6.22 (d, 1H), 10.54 (brs, 1H) MS (ES+APCI) 435.2; LCMS rétention time: 2.206 min (Method θ)
116	(R)-(1-(2-Phenyl-3H-imidazo[4,5b]pyridin-5-yl)piperidin-3yl) (py rrol idin-1 -yl)methanone	—O	’H NMR (300 MHz, CDCh) δ 1.56-1.71 (m, 2H), 1.74-2.03 (m, 6H), 2.68 (tt, 1H), 2.93 (td, 1H), 3.12 (dd, 1H), 3.37- 3.70 (m, 4H), 4.25 (d, 1 H), 4.55 (d, 1H), 6.69 (d, 1H), 7.38-7.55 (m, 3H), 7.86 (d, 1H), 7.998.09 (m, 2H), 10.63 (brs, 1H) MS (ES+APCI) (M+H) 376.4; LCMS rétention time: 2.144 min (Method G)

169

Exemple	Compound Name	-B-R²	Analytical Data
117	(fi)-( 1 -(2-(3-Chlorobenzyl)-3Himidazo[4,5-b]pyridin-5-yl)piperidin- 3-yl) (py rrolidin-l -yljmethanone		^]H NMR (400 MHz, CDCIa) δ 1.61-1.66 (m, 2H), 1.74-2.03 (m, 7H), 2.59-2.71 (m, 1H), 2.85-2.97 (m, 1H), 3.06 (dd, 1H), 3.40-3.52 (m, 3H), 3.56 (brs, 1H), 4.17-4.29 (m,2H), 4.34-4.43 (m, 1H), 6.64 (d, 1 H), 7.20 (m, 1H), 7.27-7.33 (m, 7H), 7.77 (brs, 1H), 8.80 (brs, 1H) MS (ES+) (M+H) 424.28; LCMS rétention time: 3.12 min (Method S)
118	(fi)-(1 -(2-(2-Cyclopropyloxazol-4yl)-3H-îmidazo[4,5-b]pyridin-5yl)piperidin-3-yl)(pyrrolidîn-1 yl)methanone	-fs	’H NMR (400 MHz, CDCI3) δ 1.07-1.17 (m, 4H), 1.76-2.06 (m, 8H), 2.07-2.20 (m, 1H), 2.61-2.75 (m, 1H), 2.93-3.02 (m, 1 H), 3.05-3.16 (m, 1H), 3.41-3.55 (m, 3H), 3.59-3.69 (m, 1H), 4.29 (d, 1H), 4.49 (d, 1H), 6.68 (d, 1H), 7.79 (d, 1H), 8.17 (s, 1H), 9.84 (brs, 1H) MS (ES+APCI) (M+H) 407.3; LCMS rétention time: 2.753 min (Method Z1)

170

Example	Compound Name	^R²	Analytlcal Data
119	(fî)-(1-(2-(6- (Dimethylamino)pyridin-2-y1)-3HÎmidazo[4,5-b]pyridin-5-yl)piperidin3-yl)(pyrrolidin-1-yl)methanone	N— /	MS (ES+) (M+H) 420.42 LCMS rétention time 3.15 min (Method S)
120	(fî)-(1 -(2-(6-(Azetidin-1 -yl)pyridin2-yl)-3H-tmidazo[4,5-b]pyridin-5y I) piperidin-3-yl) (py rrolidin-1 yl)methanone		’H NMR (400 MHz, CDCIa) δ 1.24-1.29 (m, 2H), 1.58-1.72 (m, 1H), 1.80-2.03 (m, 5H), 2.39-2.48 (m, 2H), 2.63-2.75 (m, 1H), 2.91-3.02 (m, 1H), 3.12 (dd, 1 H), 3.43-3.54 (m, 3H), 3.57-3.68 (m, 1 H), 4.03-4.13 (m, 4H),4.33 (d, 1H), 4.47 (d,1H), 6.31 (d, 1H), 6.65-6.72 (m, 1H), 7.52-7.58 (m, 1 H), 7.61 (brs, 1H), 7.84 (br s, 1H), 10.29 (brs, 1H) MS (ES+) (M+H) .432.3215; LCMS rétention time: 4.78 min (Method 11)

171

Example	Compound Name	^R²	Analytlcal Data
121	(fi)-(1-(2-(6- (Difluoromethoxy)pyridin-2-yl)-3Himidazo[4,5-b]pyridÎn-5-yl)piperidin3-yl)(pyiTolÎdÎn-1 -yl)methanone	O ^N=Q F	1H NMR (300 MHz, CDCI₃) δ 1.62-1.72 (m, 1H), 1.78-2.13 (m, 7H), 2.58-2.76 (m, 1 H), 2.93-3.06 (m, 1H), 3.10-3.23 (m, 1 H), 3.42-3.56 (m, 3H), 3.57-3.69 (m, 1 H), 4.35 (d, 1 H), 4.51 (d, 1H), 6.73 (d, 1H), 6.94 (d,1H), 7.51 (t,1H), 7.61-7.92 (m, 2H), 8.11 (d, 1H), 10.09 (br s, 1 H) MS (ES+APCI) (M+H) 443.0; LCMS rétention time: 2.559 min (Method G1)
122	(fi)-(1 -(2-(6- (Difluoromethyl)pyridin-2-yl)-3Himidazo[4,5-b]pyridin-5-yl)piperidin- 3-yl) (pyrrolidin-1 -yl)methanone	Λ F	MS (ES+APCI) (M+H) 427.1 LCMS rétention time 2.425 min (Method G)

172

Example _123: (fî)-Methvl 6-(5-(3-(pvrrolÎdine-1-carbonvl)piDeridin-1-vl)-1 H-imidazo[4.5blDvridin-2-vQpÎcolinate

The titie compound was prepared by a method analogous to the one used for Example 112, but using methanol as the solvent. ’H NMR (300 MHz, CDCI3) δ 1.77-2.12 (m, 8H), 2.62-2.77 (m, 1H), 2.96-3.21 (m, 2H), 3.41-3.56 (m, 3H), 3.63-3.75 (m, 1H), 4.34 (d, 1 H), 4.58 (d, 1 H), 6.73 (d, 1 H), 7.33 (d, 1 H), 7.96 (m, 1 H), 3.10 (d, 1 H), 3.48 (d, 1 H), 10.99 (br s, 1H). MS (ES+) (M+H) 434.6744; LCMS rétention time: 5.39 min (MethodH).

Example 124: ffl)-Ethvl 1-(3-(5-i3-(pvrrolidine-1-carbonyl)piperidin-1-vO-3H· imidazof4.5-b1pvridîn-2-vhphenvl)cvclopropanecarboxvlaÎe

The titie compound was prepared using a method analogous to the one used for Example 112, but using ethyl 1-(3-formylphenyl)cyclopropanecarboxylate and omitting the triethylamine. MS (ESI+)(M+H) 499.1 ; HPLC rétention time 2.6339 min (Method B).

Example 125: ffl)-Methvl 3-(5-(3-(pvrrolidine-1-carbonvl)pÎperidin-1-vl)-3H-lmidazoM.5 blpvridin-2-vhbenzoate

Into a vial was added methyl 3-formylbenzoate (102 mg, 0.6 mmol) and a solution of (fl>(1-(6-amino-5-nitropyridin-2-yl)piperidin-3-yl)(pyrrolidin-1-yl)methanone (140 mg, 0.4 mmol) in éthanol (1.5 mL). Sodium dithionate (399 mg, 2.2 mmol) and water (0.5 mL) were added. The vial was sealed and the solution was stirred 110°C for 19 h. The solution was cooled to room température and triethylamine (0.1 mL) was added. The

173 solution was stirred at 100°C for 18 h. An allquot of 0.8 mL was removed and concentrated to dryness, and the resulting residue was purified via HPLC to afford the title compound. MS (ESI+) (M+H) 434.2; HPLC rétention time 2.25 min (Method A).

Example 126: <7?)-2-Methoxv-6-(5-(3-(pvrrolÎdine-1 -carbonvl)piperidin-1 -vl)-3Hlmldazor4.5-blpvridin-2-vlÎÎsonÎcotinÎcacld

HO

To a solution of (flj-ethyl 2-methoxy-6-(5-(3-(pyrrolidine-1-carbonyl)piperidin-1-yl)-3Himidazo[4,5-b]pyridin-2-yl)isonicotinate (Example 96,50 mg, 0.1 mmol) In tetrahydrofuran (1.0 mL) was added a solution of lithium hydroxide (14.9 mg, 0.6 mmol) in methanol (1.0 mL) and water (1.0 mL). The resulting suspension was stirred at room température for 2 h. The mixture was concentrated under reduced pressure. To the residue was added a saturated solution of ammonium chloride in water (10 mL). The pH of the mixture was adjusted to 3 using aqueous hydrochloric acid (1 N). The mixture was extracted with ethyl acetate (20 mL x 3). The organics were combined, dried over sodium sulfate, filtered, and concentrated under reduced pressure. The residue was purified via HPLC to afford the title compound. MS (ESI+) (M+H) 451.1 ; HPLC rétention time 2.21 min (Method A).

Example 127: (fî)-(1-(2-(3-(MethvlsulfonvlÎphenvlÎ-3H-lmidazor4,5-blpvridin-5vl)piperidin-3-vD(pyrrolidin-1-vl)methanone

To a solution of (fîj-(1-(5,6-diaminopyridÎn-2-yl)pÎperidin-3-yl)(pyrrolîdin-1-yl)methanone dihydrochloride (125 mg, 0.3 mmol) in anhydrous Λ/,/V-dimethylformamide (2.5 mL) was added 3-(methyisulfonyl)benzaldehyde (76 mg, 0.4 mmol). The solution was stirred at

80°C for 1 h. Sulfur was added (25 mg, 0.8 mmol) followed by triethylamine (140 pL,

1.0 mmol). The mixture was stirred at 85°C for 24 h followed by stirring at room

174 température for another 24 h. The mixture was concentrated under reduced pressure and the residue was partitioned between dichloromethane and water. The aqueous layer was extracted with dichloromethane and the combined extracts were dried over magnésium sulfate, filtered and concentrated under reduced pressure. The residue was purified via flash chromatography (20-100% ethyl acetate in heptanes gradient followed by 0-10% methanol in dichloromethane). The residue was repurified via flash chromatography (0-10% methanol in dichloromethane) to afford a yellow solid that was stirred in acetonitrile (3 mL) at 40°C for 2.5 h. The solid was filtered, washed with cold acetonitrile and dried under high vacuum at 40°C to afford the title compound. ’H NMR (500 MHz, CD₃OD) δ 1.60-1.74 (m, 1H) 1.77-1.89 (m, 2H) 1.89-2.11 (m, 4H) 2.81 (brs, 1 H) 2.97-3.09 (m, 2H) 3.17-3.26 (m, 3H) 3.41-3.52 (m, 2H) 3.52-3.63 (m, 1H) 3.74 (br s, 1 H) 4.37 (br s, 1 H) 4.64 (d, 1 H) 6.91 (br s, 1 H) 7.74-7.89 (m, 2H) 8.05 (d, 1 H) 8.35 (br s, 1H) 8.65 (br s, 1H).

Example 128: ffl)-Ethvl 3-(3-(5-(3-(pvrrolidine-1-carbonyl)pîperidin-1-vl)-3H· imidazor4.5-blpvridin-2-vl)phenvlÎpropanoate

The title compound was prepared by a method analogous to the one used for Example 127, but using ethyl 3-(3-formylphenyl)propanoate as the starting material. MS (ESI+) (M+H) 476.2; HPLC rétention time 2.07 min (Method A).

Example 129: (fî)-A/,/\ADiethvl-5-(3-(pvrrolidÎne-1-carbonvl)piperidin-1-vl)-3H· imÎdazof4.5-blpvridine-2-carboxamide

Into a vial containing (f?/(1-(5,6-diaminopyridin-2-yl)pÎperidin-3-yl)(pyrrolidin-1yl)methanone dihydrochloride (125 mg, 0.3 mmol) was added formic acid (73.5 pL, 1.7 175

Φ mmol) followed by 2,2,-trifluoroethanol (1.7 mL) and methyl 2,2,2-trichloroacetimïdate (47.0 pL, 0.4 mmol). The vial was sealed and stirred at room température for 13 min. The reaction mixture was heated to 60°C and stirred for 3 h at that température. An aliquot of 0.3 mL was transferred into another vial containing diethylamine (89.1 pL, 0.9 5 mmol). The resulting mixture was heated to 60°C and stirred for 16 h. The reaction mixture was concentrated under reduced pressure and the resulting residue was partitioned between water and ethyl acetate. The aqueous was further extracted with ethyl acetate. The combined organics were concentrated under reduced pressure. The residue was purified via HPLC to afford the title compound. MS (ESI+) (M+H) 399.1 ;

HPLC rétention time 1.9864 min (Method A).

Exampie 130: ffl)-AA(Pvridin-2-vl)-5-(3-(pyrrolidine-1 -carbonvl)piperidin-1 -vl)-3Himidazof4.5-blpvridine-2-carboxamide

The title compound was prepared by a method analogous to the one used for Example 15 129, but using pyridin-2-amine as the amine. MS (ESI+) (M+H) 420.0; HPLC rétention time 2.1276 min (Method B).

Example 131 : /7?)-(1 -i2-(2-Cvclopropvlpvrimidin-4-vlÎ-3H-imidazof4.5-b1pvridin-5vlÏpiperidin-3-vl)(3,3-difluoropvnOlldin-1-vDmethanone

96, but using ethyl 2-cyclopropylpyrimidine-4-carbimidate. MS (ES-API+) (M+H) 454.0; HPLC rétention time 3.827 min (Method Q).

176

Example 132: ((F?)-1-(2-i2-CvclopropvlpvrimÎdin-4-vD-3fflmldazor4.5-blpvridin-5· vDpipendin-3-vl)((S')>3-hvdroxvpvrrolidin-1-vl)methanone

The title compound was prepared by a method anaiogous to the one used for Example 1, but using ethyi 2-cyclopropylpyrimidine-4-carbimidate. MS (ES-API+) (M+H) 434.0; HPLC rétention time 3.636 min (Method Q).

Example 133: 2-(2-CvciopTOPvlpvrimidin-4-vl)-5-(3-i6.7-dihvdro-5H-pyrrolof1.2c1imidazoi-3-vl)piperidin-1-vl)-3H-Îmidazo[4,5-b1pvridine

To a solution of (1-(5,6-diaminopyridin-2-yl)piperidin-3-yl)(pyrrolidin-1-yl)methanone dihydrochloride (18 mg, 0.06 mmol) and acetic acid (55 pL, 0.96 mmol) In éthanol (0.5 mL) was added triethylamine (17 pL, 0.12 mmol) followed by ethyi 2cyclopropylpyrimidine-4-carbimidate (11.5 mg, 0.06 mmol) dissolved in éthanol (0.5 mL). The reaction mixture was heated to 70°C for 1.25 h. The solvent was removed under reduced pressure. The residue was suspended in ethyi acetate and the undissolved solids were filtered off. The filtrate was washed with a saturated aqueous solution of ammonium chioride, washed with a saturated aqueous solution of sodium bicarbonate, and washed with brine. The organics were dried over magnésium sulfate, filtered and concentrated under reduced pressure. The crude material was purified via HPLC to afford the title compound. MS (ESI+) (M+H) 427.1 ; HPLC rétention time 2.0183 (Method A).

Examples 134 and 135: (fî)-2-(2-Cvclopropylpvrimidin-4-vl)-5-(3-(6.7-dihvdro-5HpyrroloH ,2-c1imÎdazol-3-vl)piperidin-1-vlÎ-3H-imidazof4,5-blpyridine and (S)-2-(2cvclopropvlpvrimidin-4-vi)-5-(3-i6.7-dihvdro-5M-Pvrrolon.2-c1imidazol-3-vDpiperidin-1viÎ-3fflmidazof4.5-bTpvrïdÎne

177

To a solution of 6-(3-(6,7-dihydro-5H-pyrrolo[1,2-c]imidazol-3-yl)piperidin-1-yl)pyridine-

2,3-diamine (36 mg, 0.1 mmol) in éthanol (1.0 mL) was added triethylamine (34 pL, 0.2 mmol) followed by acetic acid (111 pL, 1.9 mmol). Then a solution of ethyl 2- cyclopropylpyrimidine-4-carbimidate (23 mg, 0.1 mmol) in éthanol (1.0 mL) was added. The reaction mixture was heated to 70°C for 1.5 h. The solvent was removed under reduced pressure and the resulting residue was dissoived in ethyl acetate. The solids were filtered off and the filtrate was washed with a saturated aqueous solution of ammonium chloride, a saturated aqueous solution of sodium bicarbonate, and brine.

Then the organics were dried over magnésium sulfate, filtered and concentrated under reduced pressure to give a solid. The solid was purified via flash chromatography (0-12 % methanol in dichloromethane) to afford the title compound as a racemic mixture. The enantiomers were separated via chiral HPLC (SFC-2 instrument, Chiralcel OJ-H column 4.6 mm x 25 cm, 75/25 CCVethanol mobile phase, 0.2% isopropylamine as a modifier and a flow of 2.5 mL/min) to afford two enantiomers. Enantiomer 1 (Example 134, 3.1 mg, 6% yieid): Chiral HPLC rétention time 4.93 min (Method: Column: Chiralcel OJ-H 10 x 250; Mobile Phase 75/25 C02/Ethanol; Modifier 0.2% isopropylamine; Flow: 10.0 mL/min); ’H NMR (500 MHz, CDCI₃) δ 1.09-1.40 (m, 8H) 1.63-1.78 (m, 1H) 1.64-1.78 (m, 2H) 1.87-2.02 (m, 1H) 2.12-2.24 (m, 1H) 2.27-2.42 (m, 1H) 2.72-2.89 (m, 1H) 3.03 (br s, 1 H) 3.28-3.40 (m, 1 H) 3.56-3.68 (m, 1 H) 3.74 (d, 1 H) 4.12-4.38 (m, 2H) 6.76-6.90 (m, 1H) 7.01-7.11 (m, 1H) 7.86-8.08 (m, 2H) 8.64-8.79 (m, 1H). Enantiomer 2 (Example 135, 4.4 mg, 9% yieid): Chiral HPLC rétention time 5.41 min (Method: Same as Enantiomer 1); Ή NMR (500 MHz, CDCI₃) δ 0.84-0.92 (m, 2H) 1.14 (dd, 2H) 1.70 (d, 1H) 1.92 (d, 1 H) 2.16 (d, 1H) 2.27-2.39 (m, 1H) 2.79 (dt, 2H) 3.02 (d, 2H) 3.32 (br s, 2H)

3.54-3.78 (m, 2H) 3.98-4.72 (m, 4H) 6.81 (d, 1 H) 7.05 (br s, 1 H) 7.96 (br s, 2H) 8.68 (d,

1H).

178

Example 136: ffl)-(1-(2-(2-CvcioproDvlDvrimidin-4-vl)-6-fluoro-3H-Îmidazo[4.5-blDvridin5-vl)piperidin-3-vlÎ(pvrrolidin-1-vl)methanone

(R)-(1-(2-(2-Cyclopropylpyrimidin-4-yl)-3H-imidazo[4,5-b]pyridin-5-yl)piperidÎn-3yl)(pyrrolidin-1-yl)methanone (Example 95,100 mg, 0.2 mmol) was dissolved in N,Ndimethylformamide. The solution was cooled to 0°C and Selectfluor (87 mg, 0.2 mmol) was added. The reaction mixture was stirred at 0°C for 30 min and then at room température for 18 h. The reaction mixture was partitioned between ethyl acetate and water. The organics were washed with brine, dried over magnésium sulfate, filtered, and concentrated under reduced pressure. The residue was purified via HPLC to afford the title compound. MS (ESI+)(M+H) 436.2; HPLC rétention time 2.72 min (Method A).

Example 137: ffl)-4-Î1-(5-(3-(Pvrrolidine-1 •carbonvnp!peridin-1-vl)-3H-!rnidazof4,5b|pvridÎn-2-vl)cvclopropvl)morpholin-3-one

Step 1: AA(2-Amino-6-(3-(6,7-dihydro-5H-pyrrolo[1,2-c]imidazol-3-yl)piperidin-1yl)pyridin-3-yl)-1-(3-oxomorpholino)cyclopropanecarboxamide

Into a vial were added 1-(3-oxomorpholino)cyclopropanecarboxylic acid (33 mg, 0.2 mmol), (F?>(1-(5,6-diaminopyridin-2-yl)piperidin-3-yl)(pyrrolidin-1-yl)methanone dihydrochloride (77.5 mg, 0.2 mmol), 0-(7-azabenzotriazol-1-yl)-A/,/V,/V',/\/',tetramethyluronium hexafluorophosphate (135 mg, 0.4 mmol), diisopropylethyl amine (155 pL, 0.9 mmol) and /V,/V-dimethylformamide (3.0 mL). The reaction mixture was stirred at room température for 3 h. The mixture was directly loaded to a column and purified via flash chromatography (0-6% methanoi in dichloromethane) to afford ΛΑ(2amino-6-(3-(6,7-dihydro-5H-pyrrolo[1,2-c]imidazol-3-yl)piperidin-1-yl)pyridin-3-yl)-1-(3179 oxomorpholino)cyclopropanecarboxamide (77.0 mg, 94.7%). MS (ES+)(M+H) 457.3;

LCMS rétention time 1.56 min (Method L).

Step2: (fî>4-(1 -(5-(3-(Pyrrolidine-1 -carbonyl)piperidin-1 -yl)-3H-imidazo[4,5-b]pyridin-2yl)cyclopropyl)morpholin-3-one

The title compound was prepared by a method analogous to the one used for Example 86, Step 2. MS (ESI+) (M+H) 439.2; HPLC rétention time 1.76 min (Method A).

Example 138: (ffl-(1-(2-(2-Cvclopropvlpvrimidîn-4-ylM Wmidazof4.5-b1pvrazin-6vl)piperidin-3-vl)(pvrrolidÎn-1-vl)methanone

Step 1: 6-Bromo-2-(2-cyclopropylpyrimidin-4-yl)-1 H-imidazo[4,5-b]pyrazine A mixture of 5-bromopyrazine-2,3-diamine (50 mg, 0.3 mmol) and ethyl 2cyclopropylpyrimidine-4-carblmldate (Intermediate 19,50.7 mg, 0.3 mmol), triethylamine (73.8 pL, 0.5 mmol), acetic acid (244 pL, 4.2 mmol) and éthanol (0.8 mL) was heated to 100°C for 30 min. The mixture was concentrated and diluted with water. Then a saturated aqueous solution of sodium bicarbonate was added and the mixture was extracted with ethyl acetate. The organics were washed with brine, dried over sodium sulfate, and concentrated under reduced pressure. The crude material was purified via flash chromatography (0-5% methanol In dichloromethane) to afford 6-bromo-2-(2cyclopropylpyrimidin-4-yl)-1 H-imidazo[4,5-b]pyrazine (21 mg, 25%).

Step 2: (fî/(1 -(2-(2-Cyclopropylpyrimîdin-4-yl)-1 H-îmidazo[4,5-b]pyrazin-6-yl)piperidin3-yl)(pyrrolidin-1 -yl)methanone

A mixture of 6-bromo-2-(2-cyclopropylpyrimidin-4-yl)-1H-imidazo[4,5-b]pyrazine (86 mg, 0.3 mmol), (fi/piperidin-3-yl(pyrrolidin-1-yl)methanone (95 mg, 0.4 mmol), potassium carbonate (98.4,0.7 mmol), césium fluoride (124 mg, 0.8 mmol) and diglyme (1.0 mL) was heated to 150°C for 3 days. The mixture was diluted with water and aqueous hydrochloric acid (1N). The mixture was extracted with ethyl acetate (3x). The combined organics were washed with brine, dried over sodium sulfate, and concentrated under reduced pressure. The residue was purified via HPLC to afford the title compound. MS (ESI+) (M+H) 419.3, HPLC rétention time 2.44 min (Method A).

180

Example 139: f/7>(1-(2-i6-f1-Hvdroxvcvclopropvl)pvridin-2-vD-3H-Îmidazof4.5blpvridÎn-5-vl)ptperidin-3-vl)fpvrrolidÎn-1-vDmethanone

Step 1 : (/7)-(1 -(2-(6-(1 -((fert-Butyldimethylsilyl)oxy)cyclopropyl)pyridin-2-yl)-3Himidazo[4,5-b]pyridin-5-yl)piperidin-3-yl)(pyrrolidin-1-yl)methanone

To a solution of 6-(1-((tert-butyldimethylsilyl)oxy)cyclopropyl)picolinonitrile (45.0 mg, 0.164 mmol) in éthanol (0.3 mL) was added dropwise a solution of sodium ethoxide in éthanol (0.1 mL, 21 wt%, 0.2 mmol). The reaction mixture was stirred at room température for 50 min and then heated to 35°C for 1.5 h. To the mixture was added (/7)-(1-(5_l6-diaminopyridin-2-yl)piperidin-3-yl)(pyrrolïdin-1-yl)methanone dihydrochloride (77.2 mg, 0.2 mmol), triethylamine (47 pL, 0.3 mmol) and acetic acid (47 pL). The reaction mixture was heated to 110°C for 50 min. The solution was transferred to a microwave vial and it was heated to 145°C for 20 min. The solvent was removed under reduced pressure to afford (/7)-(1-(2-(6-(1-((tertbutyldimethylsilyOoxyïcyclopropyOpyridin^-yO-SH-imidazo^.S-bJpyridin-S-yOpiperidin-Syl)(pyrrolidin-1-yl)methanone. The material was used without further purification.

Step 2: (/7)-(1 -(2-(6-(1 -Hydroxycyclopropyl)pyridin-2-yl)-3H-imidazo[4,5-b]pyridin-5yl)piperidin-3-yl)(pyrrolidin-1-yl)methanone

Tetrabutylammonium fluoride (1M In tetrahydrofuran, 0.6 mL, 0.6 mmol) was added to a solution of (/7)-(1-(2-(6-(1 -((tert-butyldimethylsilyl)oxy)cyclopropyl)pyridin-2-yl)-3Himidazo[4,5-b]pyridin-5-yl)piperidin-3-yl)(pyrrolidin-1-yl)methanone (69.7 mg, 0.2 mmol) in tetrahydrofuran (0.5 mL). The reaction mixture was stirred at room température for 40 min. The mixture was partitioned between water and ethyi acetate (3x). The combined organics were dried over sodium sulfate and concentrated under reduced pressure. The residue was purified via HPLC to afford the title compound. MS (ESI+) (M+H) 433.1; HPLC rétention time 2.1091 min (Method B).

161

Example 140: -(2-(4-(2-Hvdroxvpropan-2-vnthlazol-2-vl)-3H-imidazo[4.5blDvndin-5-vl)piperidin-3-vl)(pvrrolidin-1-vnmethanone

Step 1 : (77/-(1 -(2-(4-Bromothiazol-2-yl)-3H-lmidazo[4,5-bJpyridin-5-yl)piperidin-3yl) (pyrrolidi n-1 -yljmethanone (flM1*(2-(4-Bromothiazol-2-yl)-3H-innidazo[4,5-b]pyridin-5-yl)piperidin-3-yl)(pyrrolÎdin-1yl)methanone was prepared by a method analogous to the one used for Example 139, Step 1, but using 4-bromothiazole-2-carbonitrile as the starting material (152 mg). ¹H NMR (400 MHz, CDCI₃) δ 1.56-2.08 (m, 8H) 2.59-2.83 (m, 1H) 2.96-3.14 (m, 1H) 3.133.26 (m, 1 H) 3.37-3.55 (m, 3H) 3.59-3.71 (m, 1H) 4.18-4.38 (m, 1H) 4.41-4.59 (m, 1H) 6.66-6.89 (m, 1H) 7.31 (s, 1H) 7.76-7.93 (m, 1H).

Step 2: (R/Methyl 2-(5-(3-(pyrrolidine-1-carbonyl)piperidin-1-yl)-3H-imidazo[4,5b]pyridin-2-yl)thiazole-4-carboxylate (77/-(1-(2-(4-Bromothiazol-2-yl)-3H-imidazo[4,5-b]pyridin-5-yl)plperidin-3-yl)(pyrrolidin-1yl)methanone (50.8 mg, 0.1 mmol), (1,1'-bls(diphenylphosphino)ferrocene)dichloropalladium (II) (13.1 mg, 0.02 mmol) and methanol (1.0 mL) were added to a vial. The vial was evacuated and filled with carbon monoxide (3x). Triethylamine (46.0 pL, 0.3 mmol) was added and the reaction mixture was stirred at 85°C for 1.5 h. The reaction mixture was directly loaded onto a silica gel column and purified via flash chromatography to afford (77/-methyl 2-(5-(3-(pyrrolidine-1-carbonyl)piperidin-1-yl)-3Himidazo[4,5-b]pyridin-2-yl)thiazole-4-carboxylate (43 mg, 89%). MS (ES+) (M+H) 441.1; LCMS rétention time 2.70 min (Method L).

Step 3: (fl/-(1 -^-^-^-Hydroxypropan^-yOthiazol^-yO-SH-imidazo^.S-bJpyridin-Syl)piperidin-3-yl)(pyrrolidin-1-yl)methanone

To a solution of (Ή/-methyl 2-(5-(3-(pyrrolidine-1-carbonyl)piperidin-1-yl)-3Himidazo[4,5-b]pyridin-2-yl)thiazole-4-carboxylate (43.0 mg, 0.1 mmol) in tetrahydrofuran (0.8 mL) at 0°C was added dropwise a solution of méthylmagnésium bromide (3M în diethyl ether, 110 pL, 0.3 mmol). The reaction mixture was stirred at 0°C for 1 h. A saturated aqueous solution of ammonium chloride was added and the mixture was

182 extracted with ethyl acetate (4 mL x 5). The combined organics were dried over sodium sulfate and concentrated under reduced pressure. The residue was purified via HPLC to afford the title compound. MS (ESI+) (M+H) 441.1; HPLC rétention time 2.04 min (Method A).

Example 141 /77)-(4-(2-(1 -(4-Chloro-1H-pvrazol-1-vl)cvclopropyl)-3H-imidazor4.5blpvridin-5-vl)morpholin-2-vl)(pvrrolidîn-1-vl)methanone

Cl

The title compound was prepared by a method anaiogous to the one used for Example 10 96. MS (ESI+) (M+H) 442.1 ; HPLC rétention time 2.1091 min (Method A).

Example 142: /7?)-(4-(2-(6-Cvc1opropvlpvridÎn-2-vl)-3H-Îmidazor4,5-blpvridin-5vl)morpholin-2-vl)(pvrrolidin-1-vl)methanone

The title compound was prepared by a method anaiogous to the one used for Example 15 112, but using Intermediate 31 and 6-cyclopropylpicolinaldehyde. MS (ES+) (M+H)

418.9; LCMS rétention time 2.411 (Method G).

Example 143: /7?)-Pvrrolidin-1 -vl(4-(2-(6-(trifluoromethvl)pvridin-2-vl)-3H-imÎdazof4,5b1pvridin-5-vl)morpholÎn-2-vl)methanone

183

112, but using 6-(trifluoromethyl)picolinaldehyde. MS (ESI+) (M+H) 447.1; HPLC rétention time 2.6238 min (Method A).

Examples 144 and 145: (FÛ-4-(2-(3-(DifluoromethoxvÎphenvl)-3H-Îmidazor4,5-blPvridin5-vl)-2-(pvridin-2-vl)morpholine and (S)-4-(2-(3-(difluoromethoxv)phenyl)-3H· ÎmÎdazof4.5-blPvridÎn-5-vl)-2-(pvridin-2-vl)morpholine

The title compounds were prepared by a method analogous to the one used for Example 112, but using 3-(difluoromethoxy)benzaldehyde to give a racemic mixture which was further purified via chiral HPLC using the following conditions: Chiralpak ASH column 10 x 250; mobile phase 80/20 carbon dioxide/ethanol; flow 10.0 mL/min to afford title compounds. Enantiomer 1 (Example 144,10% yield): Chiral HPLC rétention time: 5.67 min (Method: Column: Chiralpak AS-H 10x250; Mobile Phase: 80/20 C0₂/Ethanol; Flow: 10.0 mL/min); ¹H NMR (CDCh, 400 MHz): δ 2.96-3.01 (m, 1H), 3.16-3.22 (m, 1H), 3.92-3.96 (m, 1H), 4.14A17 (m, 1H), 4.22A25 (m, 1H), 4.60^.62 (m, 1H), 4.76 (dd, 1H), 6.61 (t, 1 H). 6.78 (d, 1H), 7.20-7.26 (m, 2H), 7.48-7.51 (m, 2H), 7.57 (d, 1H), 7.74-7.80 (m, 2H), 7.90-7.93 (m, 1H), 8.62-8.63 (m, 1H); Enantiomer 2 (Example 145,10% yield): Chiral HPLC rétention time: 7.17 min (Method: Same as for the other Enantiomer 1); ’H NMR (CDCh, 400 MHz): δ 2.95-3.03 (m, 1H), 3.15-3.26 (m, 1H), 3.91-4.01 (m, 1H), 4.14-4.21 (m, 1H), 4.22-4.28 (m, 1H), 4.58^.64 (m, 1H), 4.78 (dd, 1H), 6.62 (t, 1H), 6.80 (m, 1H), 7.20-7.26 (m, 2H), 7.48-7.54 (m, 2H), 7.57 (d, 1H), 7.72-7.80 (m, 2H), 7.90-7.95 (m, 1H), 8.60-8.64 (m, 1H).

Example 146: ffl)-(4-(2-(6-CvclopropvlpvrazÎn-2-vD-3H-imÎdazof4,5-blPvridin-5vnmorpholin-2-vlÎ(pyrrolidin-1-vl)methanone

184

The title compound was prepared by a method analogous to Example 105 using ethyl 6cyclopropylpyrazine-2-carbimidate (Intermediate 30) and ((3)-(4-(5,6-diaminopyridin-2yl)morpholin-2-yl)(pyrrolidin-1-yl)methanone (Intermediate 29). MS (ESI+) (M+H) 420.2; HPLC rétention time 2.432 min (Method A).

Exampie 147: ((3)-(1-(2-(2-Cvclobutvlpvrimldin-4-vD-3H-imidazor4.5-blpvridin-5vl)piperidin-3-vl)fpvrrolidin-1-vlÎmethanone

To a solution of ((3)-(1-(5,6-diaminopyridin-2-yl)piperidin-3-yl)(pyrrolidin-1-yl)methanone (135.9 mg, 0.5 mmol) in éthanol was added 2-cyclobutylpyrimldine-4-carbaldehyde (76.2 mg, 0.5 mmol), sulfur (30.1 mg, 0.9 mmol) and acetic acid (0.15 mL) at room température under nitrogen. The réaction mixture was stirred for 16 h at reflux. The solvent was removed under reduced pressure. Water was added to the residue and the mixture was extracted with ethyl acetate. The combined organlcs were dried over sodium sulfate and concentrated under reduced pressure. The crude material was purified via preparative TLC to afford the title compound as a yellow solid. MS (ES+APCI) (M+H) 432.1; LCMS rétention time 2.567 min (Method G).

Example 148: ((3>PvrrolidÎn-1-vl(1-(2-(2-(trifluoromethvl)pvrimÎdin-4-vl)-3H-Îmidazof4.5blpvridin-5-vl)piperidin-3-vlÎmethanone

The title compound was prepared by a method analogous to the one used for Example 147, but using 2-(trifluoromethyl)pyrimidine-4-carbaldehyde. MS (ES+APCI) (M+H) 446.2; LCMS rétention time 3.549 min (Method J).

185

Examole 149: (/?)-(1 -(2-(4-CvcloprOPvlpvrimÎdÎn-2-vlÎ-3H-lmldazor4.5-blpyridin-5vl)piperidin-3-vl)(morpholino)methanone

The title compound was prepared by a method analogous to the one used for Example 95, but using (H)-(1-(5,6-diaminopyridin-2-yl)pîperidin-3-yl)(morpholino)methanone dihydrochloride (synthesized by hyrdrogenetion analogous to the one used for Intermediate 1, starting from Intermediate 40) and Intermediate 42. MS (ES+APCI) (M+H) 434.2; LCMS rétention time 1.996 min (Method G).

Example 150: /77M-(2-(6-CvclopropvlpvridÎn-2-vD-3H-lmldazof4.5-blpvridin-5-vl)-/V,AZdimethvlpiperidine-3-carboxamide

The title compound was prepared using a method analogous to the one used for Example 112 but using ('/7}-1-(6-amino-5-nitropyridin-2-yl)-N_lN-dimethylpiperidine-3' carboxamide and 6-cyciopropylpicoiinaldehyde as starting materials. ’H NMR (300 MHz, CDCI3) δ 1.01-1.18 (m, 4H), 1.63-1.74 (m, 1 H), 1.84 (m, 1H), 1.93 (m,2H),2.10 (br s, 1 H), 2.85 (br s, 1 H), 2.96-3.03 (m, 3H), 3.08 (m, 1 H), 3.15 (s, 3H), 3.50 (s, 1 H), 4.35 (d, 1H), 4.50 (d, 1H), 6.73 (d, 1H), 7.20 (d, 1H), 7.68 (d, 1H), 7.89 (d, 1H), 8.10 (br s, 1H), 10.14 (br. s, 1H); MS (ES+APCI) (M+H) 391.2; LCMS rétention time 1.831 min (Method W).

186

• Examples 151 and 152: (S)-4-(2-(3-Chlorophenvl)-3H-lmldazof4.5-blDvridin-5-vb-2(DvridÎn-2-vl)morpholine and (/7)-4-(2-(3-chlorophenvl)-3H-Îmidazoi4.5-b1pvriciin-5-vl)-2(pyridin-2-vl)morpholîne □U

The title compounds were prepared using a method analogous to the one used for Example 112, but using 3-chlorobenzaldehyde and 3-nitro-6-(2-(pyridin-2yl)morpholino)pyridin-2-amine as starting matériels. The crude compound was purified by préparative TLC (5% acetone ln dichloromethane) to provide the racemic material, ίο which was further separated using chiral chromatography to afford title compounds.

Enantiomer 1 (Example 151,21 mg, 9% yield): MS (ES+APCI) 392.2; LCMS rétention time 2.057 min (Method G1); Chiral HPLC rétention time 8.767 min (Method: Column: CHIRAL PAK IC, 4.6 x 250 mm, 5pm; Mobile Phase A: n-Hexane; Mobile Phase C: Ethanol ; Isocratic: 75:25; Flow: 0.8 mL/min; Column Température: 25°C; ¹H NMR (300 MHz, CDCI₃) δ 2.98 (dd, 1H), 3.17 (m, 1H), 3.91 (m, 1H), 4.20 (m, 2H), 4.58 (m,

1H), 4.76 (dd, 1H), 6.78 (d, 1H), 7.26 (m, 1H), 7.41 (m, 2H), 7.55 (d, 1H), 7.75 (m, 1H), 7.88 (m, 2H), 8.02 (s, 1H), 8.63 (d, 1H). Enantiomer 2 (Example 152, 26 mg, 10% yield): MS (ES+APCI) (M+H) 392.2; LCMS rétention time 2.06 min (Method G1).

NMR (300 MHz, CDCh) δ 2.98 (dd, 1H), 3.17 (m, 1H), 3.91 (m, 1H), 4.20 (m, 2H), 4.58 20 (m, 1 H), 4.76 (dd, 1 H), 6.78 (d, 1 H), 7.26 (m, 1 H), 7.41 (m, 2H), 7.55 (d, 1 H), 7.75 (m,

1H), 7.88 (m, 2H), 8.02 (s, 1H), 8.63 (d, 1H); Chiral HPLC rétention time 10.551 min (Same method used for enantiomer 1 ).

The compounds listed ln Table 12 below were prepared using procedures analogous to those described above for the synthesis of Example 112 and using the appropriate starting materials which are available commercially or prepared using préparations well· known to those skilled ln the art or prepared by a route described above. The température of the reaction mixture for Example 153 was 100°C and for Examples 154 and 155 was 80°C.

187

Table 12

0¼

Example	Compound Name	-B-R²	Analytical Data
153	(fi)-(1-(2-(6-Cyclopropylpyridin-2yl)-3H-imidazo[4,5-b]pyridin-5yl)piperidin-3yl)(morpholino)methanone	Λ	LCMS rétention time: 1.887 min (Method F) ’H NMR (300 MHz, CDCI₃) δ 0.99-1.16 (m,4H), 1.62-1.74 (m, 1H), 1.77-1.89 (m, 2H), 1.90-1.99 (m, 2H), 2.05-2.17 (m, 1H), 2.72-2.86 (m, 1H), 3.01 (td, 1H), 3.15 (dd, 1H), 3.51-3.88 (m, 8H), 4.31 (d, 1H), 4.50 (d, IH), 6.71 (d, IH), 7.19 (d, 1H), 7.60-7.73 (m, 1H), 7.87 (d, 1H), 8.04 (d, 1H), 10.13 (br s, 1H)
154	(fi)-(1 -(2-(3-Chlorophenyl)-3Himidazo[4,5-b]pyridin-5-yl)piperidin- 3-yl) (morpholino)methanone	-Q Cl	LCMS rétention time: 2.996 min (Method A2) ’H NMR (400 MHz, DMSO-c/_e) δ 1.531.74 (m,3H), 1.85 (d, 1 H), 2.80 (d, 1H), 2.86-2.94 (m, 1H). 2.98 (dd, 1H), 3.42 (d, 1 H), 3.473.69 (m, 8H), 4.30 (d, IH), 4.40 (d,

188

			1H), 6.84 (d, 1H), 7.45-7.57 (m, 2H), 7.81 (d, 1 H), 8.07 (d, 1H), 8.13-8.18 (m, 1H), 13.03 (s, 1H)
155	(fi)-(1-(2-(3- (Difluoromethoxy)phenyl)-3Himidazo[4,5-b]pyridin-5-yl)piperidin3-yl)(morpholino)methanone	—Q _FM F	MS (ES+APCI) (M+H) 458.2; LCMS rétention time: 2.973 min (Method B2) * ’H NMR (300 MHz, CDCIg) Ô 1.55-1.72 (m, 3H), 1.82 (m, 1H), 1.86-2.00 (m, 2H), 2.74-2.87 (m, 1 H), 2.90-3.04 (m, 1H), 3.13 (dd, 1H), 3.55-3.84 (m, 6H), 4.22 (d, 1H), 4.62 (d, 1 H), 6.61 (t,1H), 6.70 (d, 1H), 7.167.24 (m, 1H), 7.49 (t, 1H), 7.86 (d, 2H), 10.35 (br.s, 1H).

Example 156: ifî>1-(6-(5-(3-(PvnOlidine-1-carbonvQpiperidin-1-vn-1 ffimidazof4,5b]pyridin-2-vl)pvridin-2-vl)cvclopropanecarbonitrile

Step 1 : (77/(1 -(2-(6-Bromopyridin-2-yl)-1 H-imidazo[4,5-b]pyridin-5-yl)piperidin-3y I) (py rrolidin-1 -yl)methanone

189 (/7)-(1 - (2-(6-B romopy ridîn-2-yl)-1 H-imidazo[4,5-b]pyridin-5-yi)piperidin-3-yl)(pyrrolidin-1 yljmethanone was prepared using a method analogous to the one used for Example 112, but using (R)-(1-(6-amino-5-nitropyridin-2-yl)piperidin-3-yl)(pyrrolidÎn-1yljmethanone and 6-bromoplcolinaldehyde as starting materials. MS (ES+APCI) (M+H) 455.0; LCMS rétention time 3.238 min (Method C1).

Step 2: (fl)-1 -(6-(5-(3-(Pyrrolidine-1 -carbonyl)piperidin-1 -yl)-1 H-imidazo[4,5-b]pyridin-2yl)pyridin-2-yl)cyclopropanecarbonitrile

To a stirred solution of (R)-(1-(2-(6-bromopyridin-2-yl)-1H-imidazo[4,5-b]pyridin-5yl)piperidin-3-yl)(pyrrolidin-1-yljmethanone (300 mg, 0.658 mmol) in toluene was added cyclopropane carbonitiile. The mixture was cooled to O’C then a 1M solution of sodium bis(trimethylsilyl)amide In THF was added dropwise. The réaction mixture was stirred for 16h at room température. The mixture was quenched with water and then was extracted with ethyl acetate. The organic layer was dried over sodium sulfate and was concentrated under reduced pressure. The residue was purified via préparative TLC (twice) to afford (R)-1-(6-(5-(3-(pyrrolidine-1-carbonyl)pîperidin-1-yl)-1H-irnidazo[4,5b]pyridin-2-yl)pyridin-2-yl)cyclopropanecarbonitrile (20 mg, 7%) MS (ES+APCI) (M+H) 442.2; LCMS rétention time 2.969 min (Method C1); ¹H NMR (400 MHz, CDCh) δ 1.24-

1.31 (m, 4H), 1.65-1.71 (m, 2H), 1.78-2.04 (m, 6H), 2.69 (m, 1H), 3.01 (t, 1H), 3.07-3.18 (m, 1H), 3.43-3.56 (m, 3H), 3.60-3.72 (m, 1H), 4.35 (br s, 1H), 4.54 (d, 1H), 6.72 (d, 1H), 7.48 (d, 1H), 7.79-7,95 (m, 2H), 8.58 (d, IH), 10.28 (br s, 1H).

Example 157: 1-(2-( 1-Phenviethvl)-3H-imidazor4.5-blDvridin-5-vDDiperidin-3vl)(pyrrolidin-1 -vDmethanone

To a solution of (/7)-(1-(5,6-diaminopyridin-2-yl)piperidin-3-yi)(pyrrolidin-1-yljmethanone dihydrochloride (Intermediate 1 ) (0.5 g, 1.7 mmol) In éthanol (20 mL) was added triethylamine (1 mL) and 2-phenylpropanal (0.25 g, 1.9 mmol). The réaction mixture was heated atreflux for 12 h. The mixture was then concentrated under reduced pressure, diluted with ice cold water and extracted with ethyl acetate. The organic layers were

190 φ dried, filtered and concentrated, and the resuiting residue was purified by préparative

TLC to afford the title compound (65 mg, 9%). MS (ES+APCI) (M+H) 404.2; LCMS rétention time 2.206 min (Method Y).

Example 158: /fî>-(1-(2-(6-CvcloDroDvlpvridin-2-vl)-3H-imidazor4.5-blpvridin-55 vl)plperidin-3-vl)(2,5-dihvdro-1 H-pyrrol-1 -vDmethanone

Step 1: (fl>Ethyl 1-(6-amino-5-nitropyridin-2-yl)piperidine-3-carboxylate

To a solution of (fl>ethyl piperidine-3-carboxylate (1 g, 6.33 mmol) ln dimethylsulfoxide (15 mL) were added 2-amino-3-nitro-6-chloropyridine (1.098 g, 6.33 mmol) and triethylamine (1.921 g, 18.99 mmol) at room température. The mixture was heated to 100°C for 18h, then was poured into Ice water and extracted with ethyl acetate. The organic layer was dried, filtered and concentrated under reduced pressure. The crude material was purified via flash chromatography (100-200 mesh silica, 30% ethyl acetate in petroleum ether) to afford (fi>ethyl 1-(6-amino-5-nitropyridin-2-yl)piperidine-315 carboxylate (650 mg, 93.5%). MS (ES+APCI) (M+H) 295.1 ; LCMS rétention time 2.832 min (Method G).

Step 2: (fi>Ethyl 1-(5,6-diaminopyridin-2-yl)piperidine-3-carboxylate

To a solution of (fi>ethyl 1-(6-amino-5-nitropyridin-2-yl)piperidine-3-carboxylate (300 mg, 1.019 mmol) in éthanol (15 mL) was added a suspension of 10% palladium-oncarbon(200 mg) in éthanol under nitrogen atmosphère. The mixture was hydrogenated using a hydrogen balloon for 2 h at room température then was filtered through a pad of Celite. The filtrate was concentrated to afford ffi>ethyl 1-(5,6-diaminopyridin-2yl)piperidine-3-carboxylate (250 mg) which was used without further purification.

Step 3: (fl>Ethyl 1-(2-(6-cyclopropylpyridin-2-yl)-3H-imidazo[4,5-b]pyridin-5yl)piperidine-3-carboxylate

To a solution of (fi>ethyl 1-(5,6-diaminopyridin-2-yl)piperidine-3-carboxylate (250 mg,

0.686 mmol) in éthanol (15 mL) was added 6-cyclopropylpicolinaldehyde (55 mg, 0.823 30 mmol), sulfur (43 mg, 1.372 mmol) and acetic acid (1.5 mL) at room température. The reaction mixture was stirred at 80°C for 18 h. The reaction mixture was poured into ice

191

V water and extracted with ethyl acetate. The organics were concentrated under reduced pressure and the crude material was purified via préparative TLC (80% ethyl acetate in petroleum ether) to afford (77)-ethyl 1-(2-(6-cyclopropylpyridin-2-yl)-3H-imidazo[4,5b]pyridin-5-yl)piperidine-3-carboxylate (180mg). MS (ES+APCI) (M+H) 392.2; LCMS rétention time 3.757 min (Method J).

Step 4: (R)-1 -(2-(6-Cyclopropylpyridin-2-yl)-3H-imidazo[4,5-bJpyridin-5-yl)piperidine’3carboxylic acid

To a solution of (R)-ethyl 1-(2-(6-cyclopropylpyridin-2-yl)-3/7-imidazo[4,5-b]pyridin-510 yl)piperidine-3-carboxylate (150 mg, 0.383 mmol) In éthanol (15 mL) was added a 2N sodium hydroxide solution (5 mL) at 0°C. The mixture was stirred at room température for 2h, then was neutralized with a 2M hydrochloric acid solution and was extracted with ethyl acetate. The organic layer was dried over sodium sulfate and concentrated under reduced pressure to afford (R)-t -(2-(6-cyclopropylpyrldin-2-yl)-3H-lmidazo[4,5-b]pyridin15 5-yl)piperidîne-3-carboxylic acid (150 mg). MS (ES+APCI) (M+H) 364.2; LCMS rétention time 2.974 min (Method J).

Step 5: ffi>(1 -(2-(6-Cyclopropylpyridin-2-yl)-3H-imidazo[4,5-b]pyridin-5-yl)piperidin-3yl) (2,5-dihydro-1 H-pyrrol-1 -yl)methanone

To a stirred solution of (R/-1 -(2-(6-cydopropylpyridin-2-yl)-3H-ÎmÎdazo[4,5-b]pyridin-5· yl)piperidine-3-carboxylic acid (150 mg, 0.686 mmol) in Ν,Ν-dimethylformamide (15 mL ) was added l-ethyl-S-ÎS-dimethylaminopropyl) carbodiimide (150 mg, 0.826 mmol), hydroxybenzotriazole (111 mg, 0.826 mmol), and 3-pyrroline (57 mg, 0.826 mmol) at room température. The reaction mixture was stirred for 24 h at room température then poured Into Ice water and extracted with ethyl acetate. The organic layer was concentrated under reduced pressure. The crude material was purified via préparative TLC (80% ethyl acetate in petroleum ether) to afford the title compound (30 mg, 17%). MS (ES+APCI) (M+H) 415.0; LCMS rétention time 2.493 min (Method G). ’H NMR (300 MHz, CDCh) δ 1.09 (m, 4H), 1.70 (m, 2H), 1.90 (m, 3H), 2.09 (m, 1H), 2.69 (m, 1H),

3.02 (m, 1 H), 3.16 (m, 1 H), 4.28 (m, 3H), 4.51 (m, 2H), 5.84 (m, 1 H), 5.93 (m, 1 H), 6.71 (d, 1H), 7.20 (d, 1 H), 7.66 (m, 1H), 7.87 (d, 1H), 8.04 (m, 1H), 10.18 (brs, 1H).

192

Example 159: ffl)-(1 -(8-(2-Cvclopropvlpvrimtdin-4-vl)-9H-purin-2-vl)piperidin-3vl)(pyrrolidin-1 -vDmethanone

Step 1: (fl)-(1-(4,5-Dtaminopyrimidin-2-yl)piperidin-3-yl)(pyrrolÎdin-1-yl)methanone To a stirred solution of (R)-(1-(4-amtno-5-nitropyrimidin-2-yl)piperidin-3-yl)(pyrrolidin-1yljmethanone (300 mg, 0.937 mmol) in éthanol (15 mL) was added a wet suspension of palladium on carbon 10% (300 mg) in éthanol under a nitrogen atmosphère. The reaction mixture was hydrogenated using a balloon filled with hydrogen gas at room température for 2 h. The suspension was filtered through celite and the filtrate was used for the next step without further purification.

Step 2: (/7/(1 -(8-(2-Cyclopropylpyrimidin-4-yl)-9H-purin-2-yl)piperidin-3-yl)(pyrrolidÎn-1 yl)methanone

To a stirred solution of (fi)-(1-(4,5-diaminopyrimidin-2-yl)piperidin-3-yl)(pyrrolidin-1yl)methanone, obtained from the previous step, in éthanol (20 mL) was added 2cyclopropylpyrimidine-4-carbaldehyde (91 mg, 0.62 mmol), sulfur (33 mg, 1.034 mmol), and acetic acid (0.5 mL). The reaction mixture was stirred at 80°C for 16 h. The reaction mixture was concentrated under reduced pressure and partitioned between water and ethyl acetate. The organics were dried over sodium sulfate, and concentrated under reduced pressure. The crude was purified via préparative HPLC and triturated with a mixture of diethyl etherpetroleum ether to afford the title compound (50 mg, 24%). MS (ES+) (M+H) 419.3070; LCMS rétention time 3.75 min (Method J1). ’H NMR (300 MHz, CDCI₃) δ 1.08-1.31 (m, 5H), 1.79-2.08 (m, 7H), 2.33 (td, 1H), 2.60 (m, 1H), 2.90-3.05 (m, 1 H), 3.07-3.22 (m, 1H), 3.40-3.56 (m, 3H), 3.59-3.73 (m, 1 H), 4.79-5.02 (m, 2H), 7.92 (d, 1H), 8.71 (d, 1H), 8.81 (s, 1H), 10.20 (brs, 1H).

193

Φ Example 160: (fî>3-(1 -(5-(3-(Pyrrolidine-1-carbonyl)piperidîn-1-yl)-3H-lmidazo[4,5bjpyri din-2-yl)cyc lopropyi) pyridi ne 1 -oxide

Step 1 : (A)-3-( 1 -(2-Amino-6-(3-(pyrrolîdine-1 -carbonyl)plperidin-1 -yl)pyrîdîn-35 ylcarbamoyl)cyclopropyl)pyridine 1-oxide

To a suspension of 3-(1-carboxycyclopropyl)pyridine 1-oxide (0.2 g, 1.1 mmol) and 2(1H-7-azabenzotriazol-1-yl)-1,1,3,3-tetramethyi uronium hexafluorophosphate (HATU) (506 mg, 1.33 mmol) in dry dichloromethane (5 mL) was added diisopropylethylamine (0.63 mL, 3.63 mmol). The mixture was stirred at room température for 10 min, and îo then Intermediate 1 (0.48 g, 1.33 mmol) was added.

After 15 min, the mixture was quenched with water and was extracted with ethyl acetate. The organic layer was washed with brine, dried over anhydrous sodium sulfate, filtered and concentrated under reduced pressure. The resulting residue was purified via flash chromatography (100-200 mesh silica, 0-10% methanol in dichloromethane) to afford (R)-3-(î -(2-amlno-6-(3-(pyrrolidine-1 -carbonyl)piperidin-1 -yl)pyridin-3ylcarbamoyl)cyclopropyl)pyridine 1-oxide (250mg). MS (ES+APCI) (M+H) 451.1; LCMS rétention time 5.133 min (Method A1).

Step 2: (fl>3-(1 -(5-(3-(Pyrrolidine-1 -carbonyl)piperidin-1 -yl)-3H-imidazo[4,5-b]pyridin-220 yl)cyc!opropyl)pyridine 1-oxide

To a solution of (R)-3-(1-(2-amino-6-(3-(pyrrolidine-1-carbonyl)piperidin-1-yl)pyridin-3ylcarbamoyl)cyclopropyl)pyridine 1-oxide (0.25 g, 0.55 mmol) in isobutanol (5 mL) was added a solution of sodium methoxide (0.15 g, 2.77 mmol) in methanol (2 mL) at room température. The reaction mixture was heated to 110°C for 16 h. The mixture was then 25 cooled to room température, diluted with ethyl acetate, and washed with water. The organic layer was dried, filtered, and concentrated under reduced pressure. The resulting residue was purified first via flash chromatography (100-200 mesh silica gel, 010% methanol ln dichloromethane) then via préparative HPLC to obtain the product which was further purified by chiral séparation to afford (R)-3-(1-(5-(3-(pyrrolidine-1 30 carbonyl)piperidin-1-yl)-3H-imidazo[4,5-b]pyridin-2-y!)cyclopropyl)pyridine 1-oxide (70 mg). Chiral HPLC rétention time: 13.808 min (Method: CHIRAL PAK IA, 4.6 x 250 mm

194 pm column, Mobile Phase: A: 0.1 % DEA In n-hexane, Mobile Phase B: éthanol;

isocratic; 70:30,1 mL/mln, Température 25 C)mln MS (ES+APCI) (M+H) 433.1; LCMS rétention time 4.698 min (Method B1). ’H NMR (400 MHz, CD₃OD) δ 1.52-1.57 (m, 2H), 1.59-1.69 (m, 1H), 1.71-1.84 (m, 4H), 1.86-2.08 (m, 5H), 2.75 (t, 2H), 2.88-3.00 (m, 2H), 3.43 (t, 2H), 3.47-3.57 (m, 1H), 3.68 (br s, 1H), 4.27 (d, 1H), 4.52 (d, 1H), 6.78 (d, 1H), 7.50-7.56 (m, 1H), 7.58-7.72 (m, 2H), 8.26 (d, 1H), 8.31 (br s, 1H).

Exampie 161: 03)-(1-(2-(1 -(Pvridin-4-vl)cvclopropvl)-3H-lmÎdazof4,5-b]PVridin-5· vlÎpiDeridÎn-3-vn(pvrrolidin-1-vlÎmethanone

The title compound was prepared by a method analogous to the one used for Example 160, using 1-(pyridin-4-yl)cyclopropanecarboxylic acid as the starting material.MS (ES+) (M+H) 417.1; LCMS rétention time 4.850 min (Method R1).

Example 162: /73)-(1-(2-i2-Cvclopropvlpvrimidin-4-vD-7-methvl-3H-imidazof4.5· blPvridin-5-vl)piperidin-3-vl)Îpvrrolidin-1-vl)methanone

Step 1 : fR>(1 -(6-Amino-4-methyl-5-nitropyridin-2-yl)piperidin-3-yl)(pyrrolidin-1 yljmethanone 03J-(1’(6’Amino-4-methyl-5-nitropyridin-2-yl)piperidin-3-yi)(pyrrolidin-1-yl)methanone was prepared using a method analogous to the one used for Intermediate 2, Step 1 but using 6-chloro-4-methyl-3-nitropyridin-2-amîne and (73J-piperidin-3-yl(pyrrolidin-1yi)methanone as starting materials. The reaction mixture was run at 70°C for 1 h. MS (ES+APCI) (M+H) 333.9; LCMS rétention time 4.322 min (Method Z).

Step 2: (73>(1 -(5,6-Diamino-4-methylpyridin-2-yl)piperidin-3-yl)(pyrrolidîn-1 yljmethanone

195 φ (Æ7/-( 1 - (5,6-Diamino-4-methylpy ridin-2-yl)piperidin -3-yl) (pyrrolidin-1 -yl)methanone was prepared using a method analogous to the one used for Intermediate 1 step 4 but without hydrogen chloride MS (ES+) (M+H) 305.0.

Step 3: (/7/-(1-(2-(2-Cyclopropylpyrimidin-4-yl)-7-methyl-3H-imidazo[4,5-b]pyridin-5yl)piperidin-3-yl)(pyrrolidin-1-yl)methanone (/7/-(1-(2-(2-Cyclopropylpyrimidin-4-yl)-7-methyl-3H-imidazo[4,5-b]pyridin-5-yl)piperidin3-yl)(pyrrolidin-1-yl)methanone was prepared using a method analogous to the one used for Example 147 at 80°C. MS (ES+) (M+H) 456.2; LCMS rétention time 2.482 min (Method G). ¹H NMR (400 MHz, CDCh) δ 1.08-1.29 (m, 5H), 1.65-1.72 (m, 1H), 1.782.08 (m, 5H), 2.24-2.35 (m, 1H), 2.62 (s, 3H), 2.64-2.74 (m, 1H), 2.99 (td, 1H), 3.14 (dd, 1H), 3.43-3.55 (m, 2H), 3.63 (dt, 1H), 4.36 (d, 1H), 4.46-4.56 (m, 1H), 6.56 (s, 1H), 7.95 (d, 1H), 8.64 (d, 1H), 10.25 (br s, 1H).

Exampie 163: iff)-(1-(2-(3-(2H-Tetrazol-5-vl)phenvl)-3H-imÎdazor4,5-bTpvridin-5vl)pÎperidin-3-vl)(pyrrolidin-1-vl)methanone

Step 1 : (R)-3-(5-(3-(Pyrrolidine-1 -carbonyl)piperidin-1 -yl)-3H-imidazo[4,5-b]pyridin-2-yl) benzonitrile

To a solution of (R)-(1 -(6-amino-5-nitropyridin-2-yl)piperidin-3-yl)(pyrrolidin-1 yl)methanone (Intermediate 1, Step 3) (50 mg, 0.15 mmol) and 3-formylbenzonitrile (34 mg, 0.31 mmol) in éthanol (2 mL) were added sodium dithionite (52 mg, 0.4 mmol), and water (0.5 mL). After 20 h at 80°C, the mixture was concentrated. The residue was partitioned between water and ethyl acetate. The organic layer was concentrated under reduced pressure and the resulting residue was purified by préparative TLC to afford (R)-3-(5-(3-(pyrrolidine-1 -carbonyl)piperidin-1 -yl)-3H-imidazo[4,5-b]pyridin-2-yl) benzonitrile as a yellow solid (40 mg, 81%). MS (ES+APCl) (M+H) 401.0; HPLC rétention time 4.016 min (Method I).

Step 2: (/7)-(1 -(2-(3-(2H-Tetrazol-5-yl)phenyl)-3H-imidazo[4,5-b]pyridin-5-yl)piperidin-3yl) (py rro lidin-1 -yl)methanone

196

V To a solution of (R)-3-(5-(3-(pyrrolidine-1 -carbonyl)piperidin-1 -yl)-3H-imidazo[4,5b]pyridin-2-y!)benzonitrile (30 mg, 0.07 mmol) in W,M-dimethylformamide (2 mL) were added sodium azide (6 mg, 0.09 mmol) and iodine (3 mg). The réaction mixture was heated for 12 h at 120°C, then was cooled. The mixture was filtered, and the filtrate s was concentrated. The resulting residue was triturated with diethyl ether to afford (R)(1-(2-(3-(2H-tetrazol-5-yl)phenyl)-3H-imidazo[4,5-b]pyridin-5-yl)piperidin-3-yl) (pyrrolidin1-yljmethanone as yellow solid (36 mg, 92%). MS (ES+APCI) (M+H) 444.1; HPLC rétention time 3.551 min (Method C1). ’H NMR (400 MHz, DMSO-cfe) δ 1.50-2.00 (m, 8H), 2.60-2.68 (m, 1H), 2.83-2.97 (m, 3H), 3.25-3.31 (m, 1H), 3.42-3.50 (m, 1H), 3.5210 3.60 (m, 1 H), 4.32 (d, 1 H), 4.42 (d, 1 H), 6.83 (d, 1 H), 7.47 (t, 1 H), 7.81 (d, 1 H), 7.948.02 (m, 2H), 8.79 (s, 1H), 13.02 (s, 1H).

Examples 164 and 165: (R)-1-(2-(2-Cvclopropvlpvrimidin-4-vl)-3H-imidazo(4_l5-blpvridin5-vl)-5.5-difluoro-/V,Mdimethvlpiperidine-3-carboxamide and (S)-1 -(2-(215 cvclopropvlpvrimldin-4-vl)-3H-imidazor4,5-blpvridin-5-vl)-5.5-difluoro-AZ,M dimethvlpiperidine-3-carboxamlde

Step 1: 1 -fert-Butyl 3-methyl 5-oxopiperidine-1,3-dicarboxylate

To a solution of dimethylsulfoxide (2.2 mL, 41.46 mmol) in dry dichioromethane (50 mL) 20 at -78°C was added dropwise at oxalyl chloride (1.57 mL, 18.2 mmol). After 10 min, a solution of 1-fert-butyl 3-methyl 5-hydroxypiperidine-1,3-dicarboxylate (4.3 g, 16.59 mmol) in dichioromethane was added dropwise. After 15 min, triethylamine (6.92 mL, 49.7 mmol) was added dropwise and the reaction mixture was then allowed to warm to room température. After 3 h, the mixture was diluted with dichioromethane and washed with water, dried over sodium sulfate, filtered and concentrated under reduced pressure to afford 1-fert-butyl 3-methyl 5-oxopiperidine-1,3-dicarboxylate (4.2 g) which was used in the next step without further purification.

Step 2: 1-fert-Butyl 3-methyl 5,5-difluoropiperidine-1,3-dicarboxylate

197

V To a solution of 1-tert-butyl 3-methyl 5-oxopiperidine-1,3-dicarboxylate (4.2 g, 16.33 mmol) in dichloromethane was added bls(2-methoxyethyl)aminosulfur trif luoride (Deoxo-fluor, 4.74 mL, 25.71 mmol). After 3 h, éthanol (0.42 mL) was added. After 16 h, the mixture was diluted with dichloromethane, washed with water, dried over sodium sulfate, filtered, and concentrated under reduced pressure. The resulting residue was purified by silica gel chromatography to afford 1-fert-butyl 3-methyl 5,5difluoropiperidine-1,3-dicarboxylate (3.0 g, 67% over two steps) as a pale yellow liquid.

Step 3: 1-(fert-Butoxycarbonyl)-5,5-difluoroplperidlne-3-carboxylic acid

To a solution of 1-tert-butyl 3-methyl 5,5-difluoropiperldine-1,3-dicarboxylate (3.0 g, 10.74 mmol) in methanol (35 mL) was added a 1N aqueous solution of sodium hydroxide (20 mL) at below 5*C. The mixture was warmed to room température, and after 16 h, the mixture was concentrated under reduced pressure. The remaining aqueous layer was acidified with a 1N aqueous solution of hydrochloric acid (to pH~4) and was extracted with ethyl acetate. The organic layer was dried over sodium sulfate, filtered, and concentrated under reduced pressure to afford 1-(fert-butoxycarbonyl)-5,5difluoropiperidine-3-carboxylic acid (2.5 g) as a pale yellow solid which was usedwithout further purification.

Step 4: fert-Butyl 5-(dimethylcarbamoyl)-3,3-difluoropiperidine-1-carboxyiate

To a solution of 1-(fert-butoxycarbonyl)-5,5-difluoropiperidine-3-carboxylic acid (2.1 g, 7.92 mmol) in W.AZ-dimethylformamide (100 mL) were added Ι,Γ-carbonyldiimtdazole (CDI, 2.56 g, 15.82 mmol), dimethylamine hydrochloride (1.29 g, 15.82 mmol) and triethylamine (2.4 g, 23.71 mmol). After 16 h, the mixture was diluted with ethyl acetate, then was washed sequentially with cold water (x2) andbrine, The organic layer was dried over sodium sulfate, filtered, and concentrated under reduced pressure. The resulting residue was purified by silica gel chromatography to afford tert-butyl 5(dimethylcarbamoyl)-3,3-difluoropiperidine-1-carboxyiate (1.4 g, 60% over two steps) as a pale yellow solid.

Step 5: 5,5-Dlfluoro-W,M-dimethylpiperidine-3-carboxamide, hydrochloric acid sait

To a solution of fert-butyl S-ÎdimethylcarbamoylJ-S.S-difluoropiperidine-l-carboxylate (1.2 g, 4.1 mmol) In diethyl ether (25 mL) was added a solution of hydrogen chloride in ether (20 mL) at below 5°C. After 15 min, the reaction mixture was warmed to room

198

W température. After 4 h, the solvent was evaporated in vacuo, and the resulting residue was triturated with diethyl ether to afford 5,5-dif!uoro-N,N-dimethylpiperidine-3carboxamide, hydrochloric acid sait (0.8 g, 86%) as a pale yellow solid.

Step 6: 1-(6-Amino-5-nitropyridin-2-y!)-5,5-difluoro-/V,Mdimethylpiperidine-3carboxamide

To a solution of 5,5-difluoro-/V,Mdimethy!piperidine-3-carboxamide (700 mg, 3.05 mmol) in dimethylsulfoxide (30 mL) were added triethylamine (619.2 mg, 6.11 mmol) and 2-amino-6-chloro-3-nitropyridine (531.1 mg, 3.059 mmol). The reaction mixture was 10 heated to 100°C. After 16 h, the mixture was cooled to room température and was partitioned between ethyl acetate and water (2x). The organic layer was washed with brine, dried over sodium sulfate, filtered, and concentrated in vacuo. The resulting residue was triturated with pentane to afford 1-(6-amino-5-nitropyridin-2-yl)-5,5-difluoro/V,AAdimethy!piperidine-3-carboxamide (0.9 g) as a yellow solid. MS (ES+APCI) (M+H) 15 330.2; LCMS rétention time: 3.066 min (Method 111).

Step 7: 1 -(5,6-Diaminopyridin-2-y!)-5,5-dif!uoro-/V,M-dimethy!piperidine-3-carboxamide To a solution of 1-(6-amino-5-nitropyridin-2-y!)-5,5-difluoro-/V,/\/-dimethylpiperidine-3carboxamide (400 mg, 1.21 mmol) in éthanol (50 mL) was added 10% palladium-on20 carbon (300 mg). After 2 h under hydrogen, the mixture was filtered through Celite which was then washed with éthanol to afford 1-(5,6-diaminopyridin-2-yl)-5,5-dif!uoro/V,Mdimethy!piperidine-3-carboxamide as a filtrate that was directly used for the next step.

Step 8: (H)-1 -(2-(2-Cyclopropylpyrimidin-4-yl)-3H-imÎdazo[4,5-b]pyridin-5-yl)-5₁5difluoro-N,W-dimethylpiperidine-3-carboxamide and (S)-1 -(2-(2-cyclopropylpyrimidin-4ylJ-SH-imidazoiA.S-bJpyridin-S-yO-S.S-difluoro-tyN-dimethylpÎperidine-S-carboxamide To a solution of 1-(5_l6-diamÎnopyridin-2-yl)-5_l5-dif!uoro-/V_tW-dÎmethy!piperidine-3carboxamide (363.5 mg, 1.21 mmol) in éthanol were added 2-cyclopropylpyrimidine-430 carbaldehyde (179.9 mg, 1.21 mmol), sulfur (77.7 mg, 2.42 mmol) and acetic acid (0.4 mL). After 16 h at reflux, the mixture was cooled and then was concentrated under reduced pressure. Oie residue was partitioned between ethyl acetate and water. The organic layer was dried over sodium sulfate, filtered, and concentrated under reduced pressure. The residue was purified by préparative TLC to afford a racemic mixture of

199 φ the desired compounds. The racemic mixture was purified via chiral préparative HPLC to afford title compounds. Enantlomer 1 (Example 164,37 mg): MS (ES+APCI) (M+H) 428.2; Chiral HPLC rétention time 5.835 min (Method: Column: CHIRAL PAK IA, 4.6 X 250mm, 5μΜ; Mobile Phase A: n-Hexane (0.1% trifluoroacetic acid); Mobile Phase C: s Ethanol; isocratic 50:50; Flow: 1.0mL/min; Column Température: 25°C).’H NMR (400 MHz, CDCI3) δ 1.11-1.25 (m, 4H), 2.26-2.42 (m, 3H), 3.03 (s, 3H), 3.04-3.15 (m, 1H), 3.23 (s, 3H), 3.25-3.37 (m, 2H), 4.56-4.71 (m, 2H), 6.78 (d, 1 H). 7.84-7.99 (m, 2H), 8.68 (d, 1H), 10.18-10.51 (m, 2H). Enantiomer 2 (Example 165, 39 mg): MS (ES+) (M+H) 428.2; Chiral HPLC rétention time 7.336 min (Method: Same as for Enantiomer 1).

Example 166: (ff)-(1-(2-(Difluoro(phenvl)methvl)-3Wmidazor4,5-blpvridin-5-vl)plperidin3-v0(pyrrolidin-1 -vDmethanone

Step 1: (R)-(1-(2-Benzyl-3H-imidazo[4,5-b]pyridin-5-yl)piperidin-3-yl)(pyrrolidin-115 yljmethanone (fl)-(1-(2-Benzyl-3H-Îmidazo[4,5-b]pyridin-5-yl)piperidin-3-yl)(pyrrolidin-1-yl)methanone was prepared by a method analogous to the one used for Example 163, Step 1 at a température of 120°C using (fi)-(1-(6-amino-5-nitropyridin-2-yl)piperidin-3-yl)(pyrrolidin1-yl)methanone and 2-phenylacetaldehyde. MS (ES+APCI) (M+H) 390.1; LCMS rétention time 3.859 min (Method I).

Step 2: (fi)-(1-(2-Benzoyl-3H-imidazo[4,5-b]pyridin-5-yl)piperidin-3-yl)(pyrTOlidin-1yl)methanone

Manganèse (IV) oxide (2 g) was added to a solution of (H)-(1-(2-benzyl-3H-imidazo[4,525 b]pyridin-5-yl)piperidin-3-yl)(pyrrolidin-1-yl)methanone (200 mg, 0.51 mmol) in dioxane (30 mL). After heating at 120°C for 48 h in a sealed tube, the mixture was filtered through a pad of Celite and washed with ethyl acetate. The filtrate was concentrated in vacuo and the resulting residue was purified via préparative TLC to afford (H)-(1-(2benzoyl-3H-imidazo[4,5-b]pyridin-5-yl)piperidin-3-yl)(pyrrolidin-1-yl)methanone (80 mg).

MS (ES+APCI) (M+H) 404.1 ; LCMS rétention time 2.855 min (Method G).

200

Step 3: R)-(1 -^-(DifluoroÎphenylJmethylJ-SH-lmidazo^.S-bJpyridin-S-ylJpIperidin-Sy I )(py rro lidin -1 -yljmethanone

Dîethylaminosulfur trifluoride (DAST, 0.5 mL) was added to a solution of (fl)-(1-(2benzoyl-3H-lmidazo[4,5-b]pyridin-5-yl)piperidin-3-yl)(pyrrolidin-1 -yl)methanone (80 mg, 0.17 mmol) in chloroform (15 mL) at 0°C. After 48 h at room température, the mixture was diluted with dichloromethane and was washed with water. The organic layer was dried over sodium sulfate, filtered, and concentrated in vacuo. The residue was purified by préparative HPLC to afford the title compound (8 mg, 11%). MS (ES+APCI) (M+H) 426.1; LCMS rétention time 3.626 min (Method C1).

Examples 167 and 168: (fî)-2-(2-Cvclopropvlpvrimidin-4-vl)-5-(3-(6.7-dihvdro-5Hpyrroloî2.1-cïï1,2,4ltriazol-3-vl)piperidin-1-vl)-3H-imldazof4,5-blDvridine and (S)-2-(2cvclopropvlpvrimidin-4-vD-5-i3-(6.7-dihvdro~5H-pvrrolof2.1-ciri.2,4ltriazol-3-vl)piperidin1 -vD-3H-lmÎdazor4,5-blpvridine

Step 1: 6-(3-(6,7-Dihydro-5H-pyrrolo[2,1-c][1,2,4]triazol-3-y1)piperidin-1-yl)pyrldine-2,3diamine, hydrochloride sait

To a solution of Intermediate 45 (300 mg, 0.91 mmol) in éthanol (50 mL) was added 10% palladium-on-carbon (200 mg). After 2 h under hydrogen, the mixture was filtered over Celite and washed with éthanol. A solution of hydrogen chloride in ether was added to the filtrate and after 10 min the mixture was concentrated under reduced pressure. The residue was triturated with diethyl ether to afford 6-(3-(6,7-dihydro-5Hpyrrolo[2,1-c][1 ^^jtriazol-S-ylJpiperidin-l-ylJpyridine^.S-dÎamine, hydrochloride sait (339 mg).

Step 2: 2-Cyclopropylpyrimidîne-4-carbonîtrile

To a solution of 2-cyclopropylpyrimidine-4-carbaldehyde (1.0 g, 6.68 mmol) ln N,Ndimethylformamide (10 mL) were added hydroxylamine hydrochloride (500 mg, 7.02 mmol) and triethylamine (1.2 mL). The reaction mixture was heated to 50°C and propylphosphonic anhydride (T3P) was added dropwise. After 16 h at 110°C, the mixture was cooled to room température, diluted with water and quenched with solid

201

V sodium bicarbonate. The mixture was extracted with ethyl acetate, then the organic layer was dried over sodium sulfate, filtered, concentrated under reduced pressure.

The residue was purified via silica gel chromatography to afford 2cyclopropylpyrimidine-4-carbonitrile (600 mg, 62%) as a pale yellow Iiquid. MS (ES+APCI) (M+H) 145.9; LMCS rétention time 2.224 min (Method G).

Step 3: Ethyl 2-cyclopropylpyrimidine-4-carbimidate

To a solution of 2-cyclopropylpyrimidine-4-carbonitrile (600 mg, 4.13 mmol) in éthanol (10 mL) was added sodium ethoxide (561.9 mg, 6.27 mmol). After 2 h, the mixture was concentrated in vacuo. The residue was partitioned between ethyl acetate and water. The organic layer was dried over sodium sulfate, filtered and concentrated under reduced pressure to afford ethyl 2-cyclopropylpyrimidine-4-carbimidate (400 mg) as a pale yellow Iiquid. The material was used without further purification.

Step 4: (fi)-2-(2-Cyclopropylpyrimidin-4-yl)-5-(3-(6,7-dihydro-5H-pyrrolo[2,1 -

c][1,2,4]triazol-3-yl)plperldin-1-yl)-3H4midazo[4,5-b]pyridineand (S)-2-(2cyclopropylpyrimidin-4-yl)-5-(3-(6,7-dihydro-5H-pyrrolo[2,1-c][1,2,4]triazol-3-yl)piperidin1 -yl)-3H-imidazo[4,5-b]pyridine

To a solution of 6-(3-(6,7-dihydro-5H-pyrrolo[2,1-c][1,2,4]triazol-3-yl)plperidin-120 yl)pyridine-2,3-diamine, hydrochloride sait (339.1 mg) in éthanol (10 mL) was added ethyl 2-cyclopropylpyrimidine-4-carbimidate (175.1 mg, 0.91 mmol) and acetic acid (2 mL). After 16 h at reflux, the mixture was cooled and was concentrated in vacuo. The residue was partitioned between ethyl acetate and water. The organic layer was dried over sodium sulfate, filtered, and concentrated under reduced pressure. Ihe residue was purified via preparative TLC to afford a racemic mixture that was further purified via chiral preparative HPLC to afford title compounds. Enantiomer 1 (Example 167,17 mg): MS (ES+)(M+H) 426.0; Chiral HPLC rétention time 10.065 min (Method: Column: CHIRALCEL ODH, 4.6 x 250mm, 5pm; Mobile Phase D: n-Hexane (0.1% DEA); Mobile Phase C: Ethanol, 70:30; Flow: 1.0 mL/min; Column Température: 25°C). ¹H NMR (300 MHz. CDCI3) δ 1.07-1.33 (m, 4H), 1.45 (d, 2H), 1.69-2.13 (m, 3H), 2.16-2.36 (m,

2H), 2.70-2.90 (m, 2H), 2.94-3.15 (m, 3H), 3.21-3.35 (m, 1H), 3.92-4.10 (m. 2H), 4.36 (d, 1H), 4.72 (d. 1H), 6.60 (d, 1H), 7.64-7.98 (m, 2H), 8.67 (d, 1H), 10.29 (br. s. 1H). Enantiomer 2 (Example 168,17 mg): MS (ES+) (M+H) 428.0; Chiral HPLC rétention time 13.738 min (Method: same as for Peak 1 ). ’H NMR (300 MHz, CDCI₃) δ 1.07-1.33

202 (m, 4H), 1.45 (d, 2H), 1.89-2.13 (m, 3H), 2.18-2.36 (m, 2H), 2.70-2.90 (m, 2H), 2.943.15 (m, 3H), 3.21-3.35 (m, 1H), 3.92-4.10 (m, 2H), 4.36 (d, 1H), 4.72 (d, 1H), 6.80 (d,

1H), 7.84-7.98 (m, 2H), 8.67 (d, 1H), 10.29 (br. s, 1H).

Example 169: 2-(6-CvclopropvlpvridÎn-2-vl)-5-(3-(6.7-dihvdro-5H-ovrrolof2,1 cli 1.2.4ltriazol-3-vl)piperidin-1 -vl)-3H-imÎdazor4,5-blpvridine

A mixture of 6-(3-(6,7-dihydro-5H-pyrrolo[2,1-c][1,2,4]triazol-3-yl)piperidin-1-yl)-3nitropyridîn-2-amine (Intermediate 45) (300 mg, 0.91 mmol), 2-cyclopropylpyridine-6aldehyde (187.5 mg, 1.27 mmol), sodium dithîonite (602.6 mg, 4.46 mmol), éthanol (15 mL) and water (2.4 mL) in a sealed tube was heated to 110°C for 16 h. The mixture was concentrated under reduced pressure and the residue was partitioned between ethyl acetate and water. The organic layer was dried over sodium sulfate, filtered, and concentrated under reduced pressure. The residue was and purified via préparative TLC to afford the title compound (80 mg, 21%) as a racemic mixture. MS (ES+) (M+H) 427.3; LCMS rétention time 2.687 min (Method J). ¹H NMR (400 MHz, CDCI₃) δ 1.021.15 (m,4H), 1.73-1.82 (m, 1H), 1.89-2.13 (m, 3H), 2.21 (d, 1H), 2.74-2.85 (m, 2H), 2.94-3.01 (m, 2H), 3.02-3.12 (m, 2H), 3.21-3.32 (m, 1H), 3.91-4.10 (m, 2H), 4.33 (d,

1H), 4.65 (d, 1H), 6.75 (d, 1H), 7.20 (d, 1H), 7.66 (t, 2H), 7.88 (d, 1H), 8.05 (d, 1H),

10.20 (brs, 1H).

Example 170 and 171: (ff)-2-(6-Cvclopropvlpvridin-2-vl)-5-(3-(6.7-dÎhvdro-5Hpyrrolof2.1-cK1,2,4ltriazol-3-vl)PÎperidin-1-vl)-3H-imîdazor4.5-b)pvridine and/or (SI-2-Î6cvclopropvlpvridÎn-2-vl)-5-(3-(6.7-dihvdro-5H-pvnOlo(2.1-ciri,2.4itriazol-3-vl)pÎperidin-1vl)-3H-imidazof4.5-b|pvridine

The title compounds were obtained via chiral préparative HPLC purification of Example

169. Enantiomer 1 (Example 170,32 mg): MS (ES+) (M+H) 427.36; Chiral HPLC rétention time 9.561 min (Method: Column: CHIRALCEL ODH, 4.6 X 250mm, 5pm;

Mobile Phase A: n-Hexane (0.1% DEA in n-Hexane; Mobile Phase C: Ethanol, 70:30;

203 φ Flow: 1 .OmL/min; Column Température: 25°C) Enantiomer 2 (Example 171,31 mg):

MS (ES+) (M+H) 427.3; Chiral HPLC rétention time 12.719 min (Method: Same as for

Peak 1). ’H NMR (400 MHz, CDCh) δ 0.98-1.18 (m, 4H), 1.90-2.14 (m, 4H), 2.16-2.26 (m, 1H), 2.73-2.89 (m, 2H), 2.94-3.12 (m, 4H), 3.19-3.31 (m, 1H), 3.92-4.10 (m, 2H),

S 4.33 (d, 1 H), 4.65 (d, 1 H), 6.75 (d, 1 H), 7.20 (dd, 1 H), 7.67 (t, 1 H), 7.89 (d, 1 H), 8.05 (d, 1H), 10.22 (brs, 1H).

Example 172: (fi)-6-(5-(3-(Pvrrolidine-1-carfaonvl)piperidin-1-vl)-3WmÎdazoM.5bÎPvridin-2-vDpicolinamide

Step 1: (fi)-Methyl 6-(5-(3-(pyrrolidine-1-carbonyl)piperidin-1-yl)-3W-imidazo[4,5b]pyridin-2-yl)picolinate (fi)-Methyl 6-(5-(3-(pyrrolidine-1 -carbonyl)piperidin-1 -yl)-3H-imidazo[4,5-b]pyridin-2yl)picolinate (150 mg) was prepared by a method anaiogous to the one used for Example 112, but using (fi)-(1-(6-amino-5-nitropyridin-2-yl)pîperidin-3-yl)(pyrrolidin-1-yl) 15 methanone (Intermediate 1, Step 3), methyl 6-formylpicolinate, and methanol as the solvent. MS (ES+) (M+H) 434.67; LCMS rétention time 5.39 min (MethodH).

Step 2: (fi)-6-(5-(3-(Pyrrolidine-1 -carbonyl)piperidin-1 -yl)-3H-imidazo[4,5-b]pyridin-2yl)picolinamide

To (fi)-methyl 6-(5-(3-(pyrrolidine-1-carbonyl)piperidin-1-yl)-3W-imidazo[4,5-b]pyridin-2yl)picolinate (200 mg, 0.460 mmol) in toluene (5 mL) were added sequentially a freshly prepared saturated solution of ammonia in dioxane (10 mL) at -20°C, and trimethylaluminum (0.099 g, 1.348 mmol). The réaction vessel was sealed and was heated at 100°C for 16 h. The mixture was cooled to room température and was partitioned between ethyi acetate, and water. The organic layer was dried over sodium sulfate and concentrated under reduced pressure.The resulting residue was triturated twice with methanokdiethyl ether (1:10) to afford the title compound (40 mg). MS (ES+) (M+H) 420.2892; LCMS rétention time 3.71 min (Method 11).

204

Exampie 173: (fî)-A/.AADimethvl-6-(5-(3-(pvrrolidine-1-carbonvl)piperidin-1-vl)-3Himidazof4,5-b)pvridÎn-2-vl)picolinamide

The title compound (40 mg, 20%) was prepared by a method anaiogous to the one used for Example 172, but for step 2 dimethylamine was used. MS (ES+) (M+H) 44Θ.1 ; LCMS rétention time 3.7Θ3 min (Method I). ¹H NMR (300 MHz, CDCh) δ 1.41-1.48 (m, 1H), 1.51-1.57 (m, 1H), 1.78-2.10(m, 7H), 2.69 (d, 1H), 3.03(dd, 1 H), 3.09 (brs, 3H), 3.20 (s, 3H), 3.39-3.58 (m, 2H), 3.59-3.73 (m, 1H), 4.29-4.39 (m, 1H), 4.52 (d, 1H), 6.73 (d, 1H), 7.59 (d, 1H), 7.82-7.97 (m, 2H), 8.33 (d, 1H), 10.24 (br s, 1H).

Example 174: (1 fî,4fî)-2-Oxa-5-azablcvcloi2.2.1lheptan-5-vl((AM-(2-(2cvciopropvlpvrimidin-4-vl)-3Wmidazof4.5-blpvridin-5-vl)piperidin-3-vl)methanone

Step 1: (R)-Methyl 1-(6-amino-5-nitropyridin-2-yl)piperidine-3-carboxylate (R)-Methyl 1-(6-amino-5-nitropyridin-2-yl)piperidine-3-carboxylate was prepared by a method anaiogous to Example 158, Step 1, but the réaction mixture température was 80°C. MS (ES+APCi) (M+H) 281.1

Step 2: (fi)-Methyi 1-(5,6-diaminopyridin-2-yl)piperidine-3-carboxylate (fi)-Methyl 1-(5,6-diaminopyridin-2-yl)piperidine-3-carboxylate was prepared by a method anaiogous to Example 158, Step 2. The material was used without further purification.

Step 3: (R)-Methyl 1-(2-(2-cyclopropylpyrimidin-4-yl)-3H-imtdazo[4_t5-b]pyridin-5yl)piperidine-3-carboxylate (fi)-Methyl 1-(2-(2-cyclopropylpyrimidin-4-yl)-3H-imÎdazo[4,5-b]pyridin-5-yl)ptperidine-3carboxyiate was prepared by a method anaiogous to Example 158, Step 3, but using 2cyclopropylpyrimidine-4-carbaldehyde. MS (ES+APCI) (M+H) 379.0.

205

Step 4: (/7)-1 -(2-(2-Cyclopropylpyrim(dîn-4-yl)-3Wnriidazo[4,5-b]pyridin-5-yl)piperidine-

3- carboxylic acid

To a solution of (/7)-methyl 1-(2-(2-cyclopropylpyrimidin-4-yl)-3H-imidazo[4,5-b]pyridin5-yl)piperidine-3-carboxylate (250 mg, 0.66 mmol) in éthanol (10 mL), was added an aqueous solution of sodium hydroxide (1N, 5 mL). The reaction mixture was stirred at room température for 2 h. The solvent was distilled off and the residue was partitioned between water and ethyl acetate. The aqueous layer was acidified with aqueous hydrochloric acid (10%) to pH-2. The resulting precipitate was collected by filtration and dried under vacuum to afford (/7)-1 -(2-(2-cyclopropylpyrimldin-4-yl)-3H-imidazo[4,5b]pyridin-5-yl)piperidine-3-carboxylic acid (200 mg). MS (ES+APCI) (M+H) 365.2

Step 5: (1 /7,4/7)-2-Oxa-5-azabicyclo[2.2.1 ]heptan-5-yl((/7)-1 -(2-(2-cyc1opropylpyrimidin-

4- yl)-3H-Îmidazo[4,5-b]pyridin-5-yl)piperidin-3-yl)methanone

To a solution of (/7)-1-(2-(2-cyclopropylpyrimidin-4-yl)-3H-imidazo[4,5-b]pyridîn-5yl)piperidine-3-carboxylic acid (70 mg, 0.192 mmol) in A/.M-dimethylformamide (10 mL) were added 1-ethyl-3-(3-dimethylaminopropyl) carboiimlde (EDCI) (73.2 mg, 0.383 mmol), hydroxybenzotriazole (52 mg, 0.383 mmol), triethylamine (58.2 mg, 0.575 mmol), (1R,5S)-8-oxa-3-azabicyclo[3.2.1]octane (186 mg, 1.64 mmol) and 4dimethylaminopyridine (3 mg) at room température. The reaction mixture was stirred for 18 h, poured into ice water, extracted with ethyl acetate, washed with cold water and concentrated. The crude was purified via préparative TLC (3% methanol in dichloromethane) to afford the title compound (9 mg). MS (ES+) (M+H) 446.3; LCMS rétention time 1.816 min (Method F) _E_xample.175: (/7)-(4-(2-(6-(Difluoromethvl)pyridin-2-vl)-3/7-imidazo[4,5-blDvridin-5vl)morpholin-2-vDfpyrrolidin-1-vnmethanone

The title compound (20 mg) was prepared by a method analogous to the one used for

Example 163, Step 1 but using 6-(difluoromethyl)picolinaldehyde and purifying the crude material by silica gel chromatography. MS (ES+) (M+H) 429.0; LCMS rétention time 4.092 min (Method I).

206

Example 176: (fî)-(1 -(2-(2-Cvclopropylpvrimidin-4-vl)-3 Wmidazor4,5-bipyridin-5vl)pipendin-3-vn(3,3-difluoroazetidin-1-vl)methanone

The title compound (26 mg, 43%) was prepared by a method analogous to the one used for Example 158 but using 3,3-difluoroazetidine for step 5.. MS (ES+) (M+H) 440.5090; LCMS rétention time 3.649 min (Method J1). ¹H NMR (300 MHz, CDCI₃) δ 1.10-1.24 (m, 4H), 1.26 (s, 1H), 1.79-1.99 (m, 3H), 2.24-2.36 (m, 1H), 2.42-2.58 (m, 1H), 2.98-3.20 (m, 2H), 4.19-4.43 (m, 3H), 4.44-4.70 (m, 3H), 6.75 (d, 1 H), 7.84-7.97 (m, 2H), 8.67 (d, 1H), 10.31 (brs, 1H).

Examples 177 and 178: i2fî.5S)-4-(2-(3-Chlorophenvl)-3fflmidazor4.5-blpyridin-5-vl)-5· methvl-2-(pvridin-2-yl)morpholine and (2S.5S)-4-(2-(3-chlorophenvD-3fflmidazor4,5b1pvridin-5-vl)-5-methvl-2-fpyridin-2-vl)morpholine

Step 1: 2-(Oxiran-2-yl)pyridine 1-oxide

To a suspension of 3-chloroperoxybenzoic acid (m-CPBA, 172 g, 1000 mmol) in dichloromethane (500 mL) was added 2-vinylpyridine (30 g, 285.7 mmol). After 30 min, the reaction mixture was heated at reflux for 36 h. The solution was then filtered and concentrated under reduced pressure. The residue was purified via neutral alumina chromatography (10% methanol in dichloromethane) to afford 2-(oxiran-2-yl)pyridine 1oxide as a white solid (4.5 g, 10%).

Step 2: 2-(2-(Benzyl((S)-1-hydroxypropan-2-yl)amino)-1-hydroxyethyl)pyridine 1-oxide To a solution of 2-(oxiran-2-yl)pyridine 1-oxide (6.6 g, 48.48 mmol) in éthanol (150 mL) were added (S)-2-(benzylamino)propan-1-ol (16 g, 96.96 mmol) and potassium carbonate (13.3 g, 96.96 mmol), After 24 h, the mixture was concentrated under reduced pressure and the resuiting residue was purified via alumina chromatography (1% methanol in dichloromethane) to afford 2-(2-(benzyl((S)-1-hydroxypropan-2yl)amino)-1-hydroxyethyl)pyridine 1-oxide as a yellow liquid (7 g, 22%).

207 φ Step 3: 2-((5S)-4-Benzyl-5-methylmorpholin-2-yl)pyridine 1 -oxide

2-(2-(Benzyl((S)-1-hydroxypropan-2-yl)amino)-1-hydroxyethyl)pyridine 1-oxide (7 g,

23.17 mmol) was dissolved in 70% sulfuric acid (70 mL) and the mixture was heated at reflux for 7 h. The mixture was cooled and then was diluted with water, neutralized with an aqueous sodium carbonate solution and extracted with ethyl acetate. The organic layer was dried over anhydrous sodium sulfate, filtered, and concentrated under reduced pressure. The residue was purified by alumina chromatography (1% methanol in dichloromethane) to afford 2-((5S)-4-benzyl-5-methylmorpholin-2-yl)pyridine 1-oxide as a yellow liquid (3 g, 45%).

Step 4: (5S)-5-Methyl-2-(pyridin-2-yl)morpholine

To a suspension of 10% palladlum-on-carbon (2 g) In éthanol (30 mL) was added a solution of 2-((5S)-4-benzyl-5-methylmorpholin-2-yl)pyridine 1-oxide (3.5 g, 13.06 mmol) in éthanol (10 mL). The mixture was subjected to a hydrogen atmosphère using a ballon filled with hydrogen gas for 2 h. The mixture was filtered through Celite and the filtrate was concentrated under reduced pressue to afford(5S)-5-methyl-2-(pyridin-2yljmorpholine (0.7 g). MS (ES+)(M+H) 179.0.

Step 5: 6-((5S)-5-Methyl-2-(pyridin-2-yl)morpholino)-3-nitropyridin-2-amlne

To a solution of (5S)-5-methyl-2-(pyridin-2-yl)morpholine (0.7 g, 3.93 mmol) in dimethylsulfoxide (20 mL) was added triethylamine (0.68 g, 7.86 mmol). After 10 min, 6chloro-3-nitropyridin-2-amine (0.68 g, 3.93 mmol) was added. After 4 h at 110°C, the mixture was cooled to room température, and then was partitioned between ethyl acetate and water. The organic layer was dried over sodium sulfate, filtered, and concentrated under reduced pressure. The residue was purified via silica gel chromatography (80% ethyl acetate in petroleum ether) to afford 6-((5 S)-5-methyl-2(pyridin-2-yl)morpholîno)-3-nitropyridin-2-amine (0.5 g, 41%).

Step 6: (2fî, 5 S)-4-(2-(3-Ch lorophenyl)-3H-im idazo[4,5-b]pyridin-5-yl)-5-methyl-230 (pyridin-2-yl)morpholine and (2S,5S)-4-(2-(3-chlorophenyl)-3H-imidazo[4₍5-b]pyridin-5yl)-5-methyl-2-(pyridin-2-yl)morpholine

To a solution of 6-((5S)-5-methyl-2-(pyridin-2-yl)morpholino)-3-nitropyridin-2-amlne (200 mg, 0.634 mmol) in éthanol (10 mL) in a sealed tube were added 3-chlorobenzaldehyde (100 mg, 0.698 mmol), sodium dithîonite (420 mg, 2.40 mmol) and water (0.8 mL). The

208 reaction mixture was heated to 110°C for 18 h. The mixture was cooled: cold water was added and the resulting mixture was stirred for 10 min. The resulting solid was isolated by filtration and was purified by préparative TLC (5% acetone in dichloromethane) to afford two diastereomers. Diastereomer 1 (Example 177,13 mg): MS (ES+) (M+H) 406.0824; LCMS rétention time 3.61 min (Method K1). Diastereomer 2 (Example 178, 21 mg): MS (ES+)(M+H) 406.1; LCMS rétention time 3.69 min (Method K1).

Example 179: (ffi-f1’f2-(5-(Methvlsulfonvnpvridin-3-vl)-3H-imidazor4.5-b|pvridin-5v0piperidin-3-vl)(pvrrolidÎn-1-vl)methanone

Step 1 : (R)-(1-(2-(5-Bromopyridin-3-yl)-3H-imidazo[4_l5-b]pyridin-5-yl)piperidin-3yl) (pyrrolidin-1 -yl)methanone

To a solution of (R)-(1-(4-amino-5-nitropyrimidin-2-yl)pÎperidin-3-yl)(pyrrolidin-1yl)methanone (0.5 g, 1.5 mmol) in a mixture of éthanol and water were added 5bromonicotinaldehyde (0.35 g, 1.6 mmol) and sodium dithionate (1.1 g, 5.9 mmol). The reaction mixture was heated to 120°C for 16 h. The reaction mixture was concentrated and partitioned between water and ethyl acetate. The organics were concentrated under reduced pressure and the resulting crude was purified via préparative TLC to afford (R)-(1-(2-(5-bromopyridin-3-yl)-3H-lmidazo[4,5-b]pyridin-5-yl)piperidin-3yl)(pyrrolidin-1-yl)methanone (400 mg, 60%).

Step 2: (R)-(1 -(2-(5-(Methylsulfonyl)pyridin-3-yl)-3H-imidazo[4₁5-b]pyridÎn-5-yl)pÎperidin3-yl) (pyrrolidin-1 -yl)methanone

To a solution of R)-(1-(2-(5-bromopyridin-3-yl)-3H-imidazo[4,5-b]pyridin-5-yl)piperidin-3yl)(pyrrolidin-1-yl)methanone (0.05 g, 0.1 mmol) in dimethylsulfoxide was added sodim methylsulfinate (NaSO₂Me) (13 mg, 0.13 mmol), copper iodide, L-proline and sodium hydroxide. The réaction mixture was heated to 90°C for 14 h. The reaction mixture was partitioned between water and ethyl acetate. The organics were concentrated under reduced pressure and the resulting crude was purified via préparative TLC to afford the title compound (25 mg, 45%). MS (ES+APCI) (M+H) 455.0; LCMS rétention time: 3.859 min (Method l).

209

Example 180 and 181: (3/?^/7)-1-(2-(3-(DifluoiOmethoxv)phenvl)-3H-imidazor4,5bipvndin-5-vn-/v./v.4-tnmethvlpipendîne-3-can)oxamlde and (3S.4Sb1-(2-(3(difluoromethoxvÎphenvl)-3Wmidazor4,5-b]pyridin-5-vl)-A/.A/,4-trimethvlpiperidine-3carboxamide

Step 1: cîs-fert-Butyl 3-(dîmethylcarbamoyl)-4-methylpiperidine-1-carboxylate To a solution of cis-1-(fert-butoxycarbonyl)-4-methylpiperidine-3-carboxylic acid (prepared by /V-Boc protection of c/s-4-methylpîperidîne-3-carboxylic acid, synthesized by the method described in Bulletin de la Société Chimique de France 1986, 4,663-8.) (0.4 g, 1.64 mmol) in tetrahydrofuran were added triethylamine (0.2 mL, 1.64 mmol), dimethylamine hydrochloride (132 mg, 1.64 mmol), and Ι,Γ-carbonyldiimidazole (CDI, 265 mg, 1.64 mmol). After 2 h, the mixture was quenched with water and the aqueous layer was extracted with ethyl acetate (2x10 mL). The combined organic layers were dried over sodium sulfate, filtered and concentrated under reduced pressure to afford cis-fert-butyl 3-(dimethylcarbamoyl)-4-methylpiperidine-1 -carboxylate (0.3 g, 68%) as a yellow solid. The material was used without further purification.

Step 2: cis-N,N-4-Trimethylpiperidine-3-carboxamide hydrochloride

To cis-tert-butyl 3-(dimethylcarbamoyl)-4-methylpiperidine-1-carboxylate (0.3g, 1.12 mmol) was added a solution of hydrogen chloride in ether. The mixture was stirred for 30 min, and then was concentrated under reduced pressure to afford cis-W,M,4trimethylpiperidine-3-carboxamide hydrochloride (0.16 g) as a white solid. The material was used without further purification.

Step 3: cis-1 -(6-Amino-5-nitropyridin-2-yl)-M,W,4-trimethylpiperidine-3-carboxamide To a solution of W,W,4-trimethylpiperidine-3-carboxamide (0.16 g, 0.96 mmol) in dimethylsulfoxide (5 mL) were added triethylamine (0.2 mL, 0.96 mmol) and 6-chloro-3nitropyridin-2-amine (0.168 g, 0.96 mmol). The reaction mixture was heated for 12 h at 80°C, then was cooled. The mixture was diluted with water and the aqueous layer was extracted with ethyl acetate (2x10 mL). The combined organic layers were dried over sodium sulfate, filtered, and concentrated under reduced pressure. The residue was purified via silica gel chromatography to afford cis-1-(6-amino-5-nitropyridin-2-yl)-M,W,4trimethylpiperidine-3-carboxamide (0.2 g, 68%).

210

Step 4: (3R,4RJ-1 •(2-(3-(Difluoromethoxy)phenyl)-3H-lmidazo[4,5-b]pyridin-5-yl)-/V,A/₁4’ tnmethylpiperidine-3-carboxamide and (3S,4S)-1 -(2-(3-(difluoromethoxy)phenyl)-3HimÎdazo[4,5-b]pyridÎn-5-yl)-A/,A/,4-trimethylpiperidine-3-carboxamide

The title compounds were prepared by a method analogous to the one used for Example 163, Step 1 using N,/V,4-trimethylpiperidine-3-carboxamide and 6-chloro-3nitropyridin-2-amine to afford a racemic mixture that was further purified via chiral HPLC to obtain enantiomer 1 and enantlomer 2. Enantiomer 1 (Example 180, 8.4 mg): MS (ES+APCI) (M+H) 430.2; LCMS rétention time 2.965 min (Method P1). ¹H NMR (300 MHz, DMSO-cfe) δ 0.86 (d, 1H), 0.95 (d, 3H), 1.50-1.62 (m, 1H), 1.73-1.86 (m, 1H), 2.27 (m, 1H), 2.68-2.75 (m, 1H), 2.82 (br s, 3H), 2.95-3.06 (m, 3H), 3.34-3.50 (m, 1 H), 3.904.08 (m, 2H), 6.80 (d, 1H), 7.21 (d, 1H), 7.32 (t, 1H), 7.55 (d, 1H), 7.79 (d, 1H), 7.887.92 (m, 1H), 7.97 (d, 1H), 13.09 (s, 1H). Enantiomer 2 (Example 181,6.6 mg): MS (ES+APCI) (M+H) 430.3; LCMS rétention time 2.963 min (Method P1).

Example 182: (fi)-(1-(2-(6-Cvcloproovlpvridin-2-vl)-3Wmidazor4.5-blpyridin-5vl)piperidin-3-vl)(pyrrolidin-1-vl)methanone

To a solution of (R)-(1-(6-amino-5-nitropyridin-2-yl)piperidin-3-yl)(pyrrolidin-1yl)methanone (250 mg, 0.711 mmol) In éthanol (15 mL) was added 6cyclopropylpicolinaldehyde (0.137 g, 0.974 mmol), sodium dithionite (536 mg, 2.96 mmol) and water (2 mL). The reaction mixture was stirred for 16 h at 100°C in a sealed tube. The mixture was cooled, and the solvent was removed under reduced pressure. Water was added to the residue and the mixture was extracted with ethyl acetate. The organics were dried over sodium sulfate, filtered and concentrated under reduced pressure. The crude material was purified via préparative TLC eluting with 4% methanol in dichloromethane to afford the title compound (55 mg, 17%). MS (ES+APCI) (M+H) 417.2; LCMS rétention time 1.944 (Method F). ¹H NMR (300 MHz, CDCI₃) δ 1.00-1.09 (m,2H), 1.09-1.16 (m, 2H), 1.63-1.74 (m, 1H), 1.81-2.14 (m, 7H), 2.62-2.76 (m, 1H), 2.99 (dd, 1H), 3.08-3.21 (m, 1H), 3.44-3.55 (m, 3H), 3.58-3.70 (m, 1H), 4.35 (d, 1H), 4.48 (d, 1H), 6.71 (d, 1H), 7.19 (d, 1H), 7.66 (t, 1H), 7.87 (d, 1H), 8.04 (d, 1H), 10.18 (brs, 1H).

211

Exampie 183: (/?)-( 1-(2-(5-CvcloproDvlpvridin-3-vl)-3H-lmidazoi4,5-b1pyridin-5· vl)pipendin-3-vl)(pvrrolidin-1-vDmethanone

To a solution of ethyl 5-cyclopropylnicotinimidate (0.2 g, 1.04 mmol) In éthanol was added acetic acid (0,1 mL), triethylamine (0.1 mL) and a solution of 03)-(1-(5,6diaminopyridin-2-yl)piperidin-3-yl)(pyrrolidin-1-yl)methanone dihydrochloride sait (0.4 g, 1.1 mmol) in éthanol. The reaction mixture was heated at reflux for 24 h. The solvent was removed under reduced pressure and to the resulting residue was added a saturated aqueous solution of ammonium chloride. The mixture was extracted with ethyl acetate. The organic layer was concentrated under reduced pressure. The crude material was purified via préparative TLC to afford the title compound (0.025 g). ’H NMR (400 MHz, DMSO-cfc) δ 0.85 (br s, 2H), 1.07 (d, 2H), 1.47-1.74 (m, 4H), 1.74-1.96 (m, 5H), 1.98-2.10 (m, 1H), 2.61 (brs, 2H), 2.92 (d, 2H), 3.41-3.59 (m, 2H), 4.33 (d, 1 H), 4.39 (d, 1 H), 6.86 (d, 1 H), 7.81 (d, 1 H), 8.00 (br s, 1 H), 8.47 (br s, 1 H), 9.05 (br s, 1H), 13.07 (brs, 1H). MS (ES+) (M+H)417.36; LCMS rétentiontime3.03 min (Method S).

Example 184: (FA-(1 -f2-(6-Cvclopropylpvridin-2-vB-3H-imidazof4.5-b]pvridin-5vl)piperidin-3-vl)(3,3-difluoropvrrolidÎn-1-vl)methanone

The title compound was prepared by a method analogous to the one used for Example 158, but using 3,3-difluoropyrrolidine instead of 3-pyrroline and 4-dimethylaminopyridine for the final step. ’H NMR (300 MHz, CDCb) δ 1.01-1.17 (m, 4H), 1.78-2.01 (m, 3H), 2.02-2.16 (m, 2H), 2.31-2.61 (m, 3H), 2.65-2.78 (m, 1H), 3.01-3.21 (m, 2H), 3.66-4.00 (m, 3H), 4.24 (d, 1H), 4.54 (t, 1H), 6.71 (dd, 1H), 7.20 (dd, 1H), 7.58-7.71 (m, 1H), 7.88 (d, 1H), 8.04 (d, 1 H), 10.17 (br s, 1H). MS (ES+APCI)(M+H) 452.9; LCMS rétention time 4.371 min (Method I).

212 φ Example 185: (/7)-1-(2-(2-CvclopropvlpvrimÎdin-4-vl)-3Wmidazo[^,4.5-blpyridin-5-vl)-/\/· ethvl-A/-methvlpiperidine-3-carboxamide

To a solution of (/7)-1-(5₁6-diaminopyridin-2-yl)-/V-ethyl-/V-methylpiperidine-35 carboxamide (200 mg, 0.722 mmol) in éthanol (10 mL) was added 2cyclopropylpyrimidine-4-carbaldehyde (106 mg, 0.722 mmol), sulfur powder (69 mg, 2.16 mmol), and acetic acid (0.2 mL). The reaction mixture was heated to 80°C for 18 h. The solvent was evaporated and the residue was partltioned between ethyl acetate and water. The organic layer was concentrated under reduced pressure. The crude material was purified via préparative HPLC to afford the title compound (40 mg, 13.5%). Ή NMR (300 MHz, CDCI₃) δ 1.09-1.18 (m, 3H), 1.19-1.31 (m, 4H), 1.65-1.73 (m, 1H), 1.84 (d, 1 H), 1.88-1.98 (m, 2H), 2.31 (d, 1 H), 2.69-2.87 (m, 1 H), 2.99 (br s, 1 H), 3.03 (br s, 1H), 3.11 (brs, 1 H), 3.14-3.27 (m, 1H), 3.37-3.57 (m, 2H), 4.37 (d, 1H), 4.45-4.59 (m, 1H), 6.76 (d, 1H), 7.79-7.96 (m, 1H), 8.67 (d, 1H), 10.25 (brs, 1H). MS (ES+APCI) (M+H) 406.3; LCMS rétention time 3.055 min (Method U1).

Example 186: (fî)-Î4-(2-(6-Cvclopropvlpvridin-2-vlÎ-3ffimldazof4.5-b1Pvridin-5yl)morpholin-2-vl)(morpholino)methanone

The title compound was prepared by a method analogous to the one used for Example 112, but the organic extract was only washed with water instead of a saturated aqueous solution of ammonium chloride and a saturated aqueous solution of sodium bicarbonate. ’H NMR (300 MHz, CDCI3) δ 1.02-1.17 (m, 4H), 1.99-2.16 (m, 2H), 3.14-

3.31 (m, 1H), 3.38 (dd, 1H), 3.50-3.62 (m, 2H), 3.66-3.84 (m, 7H), 4.01-4.17 (m, 2H),

4.20-4.36 (m, 2H), 6.74 (d, 1H), 7.21 (d, 1H), 7.67 (t, 1H), 7.92 (d, 1H), 8.01-8.09 (m,

1H), 10.23 (brs, 1H). MS (ES+APCI) (M+H) 435.2; LCMS rétention time 2.322 min (Method G).

213

Example 187: (Fn-Pvrrolidin-1-vl(1-(8-(6-(trifluoromethvl)pyridin-2-vl)-9/-/-Durin-2vl)piperidin-3-vDmethanone

To a solution of ethyl 6-(trifluoromethyl)picolinimidate (291 mg, 1.3 mmol) and triethylamine (0.6 mL, 4.4 mmol) in éthanol (5 mL) was added dropwise a solution of (fî>(1-(4,5-diaminopyrimidin-2-yl)piperidin-3-yl)(pyrrolidin-1-yl)methanone dihydrochloride (400 mg, 1.1 mmol) and acetic acid (0.3 mL, 6.6 mmol) în éthanol (10 mL) at room température over a period of 10 min. The reaction mixture was stirred for 16 h at 120°C. The mixture was concentrated and the residue was partitioned between ethyl acetate and water. The organic layer was dried over sodium sulfate, filtered and concentrated under reduced pressure. The crude material was purified via preparative TLC to afford the title compound (110 mg, 20%). ¹H NMR (400 MHz, DMSO-cfe) δ 1.18-

1.32 (m, 1H), 1.48 (d, 1H), 1.71 (t, 2H), 1.76-1.83 (m, 2H), 1.89 (dt, 3H), 2.55-2.64 (m, 2H), 2.85-3.03 (m, 2H), 3.42-3.50 (m, 1H), 3.51-3.63 (m, 1H), 4.76 (d, 2H), 8.00 (d, 1H), 8.22-8.32 (m, 1H), 8.49 (d, 1H), 8.83 (brs, 1H), 13.41 (brs, 1H). MS (ES+) (M+H) 446.33; LCMS rétention time 3.34 min (Method S).

Example 188: (fîi-(1-(2-(2-(4-Chloro-1 H-pvrazol-1-vlÎpropan-2-vl)-3H-imidazof4,5b1pvridin-5-vlÏpÎperÎdin-3-vDÎpyrrolldin-1-vhmethanone ci

Step 1 : (fî>(1-(5,6-Diaminopyridin-2-yl)piperidin-3-yl)(pyrrolidin-1-yl)methanone A solution of (fi)-(1-(6-amino-5-nitropyridin-2-yl)piperidin-3-yl)(pyrrolidin-1-yl)methanone (1.5 g, 4.7 mmol) in éthanol (20 mL) was added to a suspension of 10% palladium-oncarbon (800 mg) at room température. The mixture was hydrogenated for 4 h, filtered through Celite and washed with éthanol. The filtrate was used for the next step without further purification.

214 φ Step 2: (fi)-(1-(2-(2-(4-Chloro-1 H-pyrazol-1-yl)propan-2-yl)-3H-imidazo[4,5-blpyridin-5yl)piperidin-3-y!)(pyrro!idin-1-yl)methanone

Acetic acid (2.9 mL, 43.53 mmol) and ethyl 2-(4-chloro-1 H-pyrazol-1-yl)-2methylpropanimidate (Intermediate 54) were added to a solution of (fi>(1 -(5,65 diaminopyridin-2-yl)piperidin-3-yl)(pyrrolidin-1-yl)methanone in éthanol, prepared in the previous step. The reaction mixture was heated at reflux for 18 h. The mixture was concentrated and the residue was partitioned between ethyl acetate and water. The organic layer was dried over sodium sulfate, filtered and concentrated. The crude material was purified via column chromatography (100-200 mesh silica gel, 5% methanol in ethyl acetate) to afford the titie compound (530 mg, 25% yield over 2 steps).. ’H NMR (500 MHz, CDCI₃) δ 1.53-1.65 (m, 1H) 1.74-2.00 (m, 7H) 2.10 (s, 6H) 2.58-2.70 (m, 1H) 2.88-2.93 (m, 1H) 3.01-3.09 (m, 1H) 3.40-3.50 (m, 3H) 3.55-3.63 (m, 1H) 4.22 (d, 1H) 4.43 (d, 1H) 6.63 (d, 1H) 7.57 (d, 2H) 7.79 (d, 1H) 10.08 (br s. 1H). MS (ES+APCI) (M+H) 442.2; LCMS rétention time 2.216 min (Method N1).

Example 189: (/7)-(1-(2-( 1 -(4-(MethylthÎo)-1 H-pyrazol-1 -vl)cvclopropvlÎ-3H-imidazof4,5b1pvridin-5-vl)piperidin-3-vl)(pvrrolidln-1-vl)methanone

To a solution of (fi)-(1-(5,6-diaminopyridin-2-yl)piperidin-3-yl)(pyrrolidin-1-yl)methanone 20 dihydrochloride (189 mg, 0.653 mmol) and ethyl 1 -(4-(methylthio)-1 H-pyrazol-1 yl)cyclopropanecarbimidate (157 mg, 0.697 mmol) in éthanol (1.86 mL) was added acetic acid (0.372 mL) followed by triethylamine (0.545 mL, 3.91 mmol). The reaction mixture was heated to 110°C for 2 h. The mixture was cooled to room température, the solvent was removed under reduced pressure and the residue was partitioned between 25 a saturated aqueous solution of ammonium chloride (100 mL) and dichloromethane (200 mL). The aqueous layer was extracted with dichloromethane (100 mL) and the combined organics were dried over sodium sulfate, filtered and concentrated under reduced pressure. The crude material was purified via HPLC to afford the titie compound.. MS (ESI+) (M+H) 452.2; HPLC rétention time 2.11 min (Method A).

215 • Examples 190 and 191: r(R)-T-(2-i1-(4-Chloro-1H-pvrazol-1-vl)cvcloDroDvll-3H· im1dazof4,5-b1pvridin-5-vlÎ(2,2,6,6-^gH4ÎPiperidÎn-3-vl1(pvrrolidin-1-vl)meÎhanone and KSJΚ2-Γ1 -(4-chloro-1 H-pyrazol-1 -vncvclopropvl1-3H-lmîdazoT4.5-b1pvridin-5-vlK2,2,6,6*H4)plperidin-3-vll(pvnOlidin-1-vDmethanone

Cl ci

Step 1: 1-Nitrosoplperidine-3-carboxylic acid

Aqueous hydrochloric acid (2N, 20 mL) was added to piperidine-3-carboxylic acid (5.02 g, 38.87 mmol). The mixture was cooled with an Ice bath and a solution of sodium nitrite (2.78 g, 40.29 mmol) în water (4 mL) was added. The reaction mixture was stirred at room température for 15 h. The mixture was extracted with dichioromethane (20 mL x 3). The combined organîcs were washed with brine (2x), solids were removed by filtration and the filtrate was concentrated under reduced pressure to afford 1nitrosopiperidine-3-carboxylic acid (4.5 g). MS (EI+)(M+) 158.

Step 2: (2₁2,6₁6-²H4)Piperidine-3-carboxylic acid

Aqueous sodium deuteroxide (IN, 35 mL) was added to 1-nitrosopiperidine-3-carboxylic acid (2.50 g, 15.32 mmol). The reaction mixture was heated at 70°C for 48 h. The mixture was cooled to room température and the pH was adjusted to 3 using aqueous deuterated hydrochloric acid. The mixture was extracted with dichioromethane (30 mL 20 x 5), and the combined organîcs were dried over sodium sulfate/magnesium sulfate, filtered and concentrated under reduced pressure to give a solid (0.81 g) The aqueous layer was concentrated under reduced pressure and residual solvent was azeotropicaliy distilled with methanol-d₄ (5 mL x 2). The residue was heated at 50°C in methanol-d₄(15 mL) for 30 min and then cooled to room température. The insoluble solids were removed by filtration and the filtrate was evaporated and dried to give a solid (1.65 g). Both lots of isolated solids were combined, and a solution of sodium deuteroxide (0.5 M, 2.5 sodium deuteroxide in 45 mL of deuterated water (D₂O)) was added. The mixture was heated to 75°C for 2 h, then was cooled to room température and then to 0°C.

Aluminum-nickel alloy (11 g) was slowly added. The reaction mixture was stirred at room température for 3 days, then was heated at 35°C for 3.5 h. The mixture was

216 cooled to room température and the solids were removed by filtration. The solids were washed with deuterated water (D₂O) (3 mL x 3). The pH of the filtrate was adjusted from 0 to 3 and the solution was concentrated under reduced pressure at 50°C and then the resulting residue was dried under vacuum at 40°C for 18 h. The dried residue was stirred at 40°C in deuterated methanol-di (12 mL). The mixture was cooled to room température and the solids were removed by filtration. The filtrate was concentrated under reduced pressure to afford (2,2,6,6-²H₄)piperidine-3-carboxylic acid (1.65 g).

Step 3: Ethyl (2,2_l6_l6-*H₄)piperidine-3-carboxylate

To a suspension of (2,2,6,6-²H4)piperidine-3-carboxylic acid (500 mg, 2.94 mmol) in éthanol at 0°C was added thïonyl chloride (0.6 mL, 8.84 mmol). The reaction mixture was stirred at room température for 3 h. Thionyl chloride was removed under reduced pressure to afford ethyl (2,2,6,6-²H4)piperidine-3-carboxylate (500 mg). The material was used without further purification.

Step 4: Ethyl 1-(6-amino-5-nitropyridin-2-yl)(2,2,6,6-²H4)piperidine-3-carboxylate

To a solution of ethyl (2,2,6,6-*H4)pîperidine-3-carboxylate (500 mg, 2.53 mmol) and triethylamine (1 mL, 7.59 mmol) in acetonitrile (15 mL) was added 6-chloro-3nitropyridine (306 mg, 1.77 mmol). The reaction mixture was stirred for 3 h at 80°C.

The mixture was partitioned between ethyl acetate and water. The organic layer was dried over sodium sulfate, filtered and concentrated under reduced pressure. The crude material was purified via column chromatography using 100-200 mesh silica gel (2040% ethyl acetate in petroleum ether) to afford ethyl 1-(6-amino-5-nitropyridin-2yl)(2,2,6,6-*H₄)piperidine-3-carboxylate (500 mg).

Step 5: Ethyl 1-(5,6-diaminopyridin-2-yl)(2,2,6,6-*H4)piperidine-3-carboxylate

To a suspension of 10% palladïum-on-carbon (300 mg) in éthanol (20 mL) was added ethyl 1-(6-amino-5-nitropyridin-2-yl)(2,2,6,6-²H₄)piperidine-3-carboxylate (500 mg, 1.67 mmol). The reaction mixture was submitted to an atmosphère of hydrogen for 4 h at 30 room température. The mixture was filtered through Celite and was washed with éthanol to afford ethyl 1-(5,6-dïaminopyridin-2-yl)(2,2,6,6-²H4)piperidine-3-carboxylate which was used without further purification.

217

V Step 6: Ethyl 1 -(2-(1 -(4-chloro-1 H-pyrazol-1 -yl)cyclopropy1]-3H-imidazo[4₍5-b]pyridin-5yl}(2,2,6,6-²H<)plperidine-3-carboxylate

To the filtrate containing ethyl 1-(5,6-diaminopyrldin-2-yl)(2_l2,6,6-²H4)piperidine-3carboxylatefrom the previous step was added ethyl 1-(4-chloro-1H-pyrazol-15 yljcyclopropanecarbimîdate (Intermediate 57), acetîc acid (0.5 mL) and sulfur (2 mg) at room température. The reaction mixture was heated at reflux for 18 h. The solvent was removed under reduced pressure and the residue was partitioned between ethyl acetate and water. The organic layer was dried over sodium sulfate, filtered and concentrated under reduced pressure. The crude material was purified via column chromatography using 100-200 mesh silica gel (30-70% ethyl acetate in petroleum ether) to afford ethyl

-(2-( 1 -(4-chloro-1 H-pyrazol-1 -yl)cyclopropyl]-3H-imidazo[4,5-b]pyridin-5-yl}(2,2,6,6²H4)piperidine-3-carboxylate (500 mg).

Step 7: 1 -{2-[1 -(4-Chloro-1 H-pyrazol-1 -y1)cyclopropyl]-3Wmidazo[4,5-b]pyridin-515 yl}(2,2,6,6-²H4)piperidine-3-carboxylic acid

Lithium hydroxide (146 mg, 3.57 mmol) was added to a solution of ethyl 1-(2-(1-(4chloro-1H-pyrazol-1-yl)cyc1opropyl]-3H-imidazo[4₍5-ù]pyridin-5-yl}(2_l2₍6,6²H₄)piperidine-3-carboxylate (500 mg, 1.19 mmol) in a mixture of tetrahydrofuran:water (15 mL) at room température. The reaction mixture was stirred for 2 h. Tetrahydrofuran 20 was removed under reduced pressure and the aqueous layer was diluted with aqueous hydrochloric acid (2N) until the pH was 6. The aqueous layer was extracted with ethyl acetate (2x). The combined organics were dried over sodium sulfate, filtered and concentrated under reduced pressure to afford 1-{2-[1-(4-chloro-1H-pyrazol-1yl)cyclopropyl]-3H-imidazo[4,5-d]pyridin-5-yl}(2,2,6₁6-²H4)piperidine-3-carboxylic acid 25 (300 mg).

Step 8: [(R)-7-(2-(1 -(4-Chloro-1 H-pyrazol-1 -yl)cyclopropyl]-3H-imidazo(4,5-b]pyridin-5yl)(2,2,6_l6-²H4)piperidin-3-yl](pyrrolidin-1-yl)methanone and [(S)-ï-{2-[1-(4-ch1oro-1 Hpyrazol-1-y1)cyclopropyl]-3H-imidazo[4,5-b]pyridin-5-y1}{2,2,6,6-²H4)piperidin-330 yl](pyrrolidin-1-yl)methanone

Pyrrolidine (0.1 mL, 0.25 mmol) was added to a solution of 1-{2-[1-(4-chloro-1H-pyrazol1-yl)cyclopropyl]-3H-imidazo[4,5-ù]pyridin-5-yl}(2,2,6,6-²H₄)piperidine-3-carboxylÎc acid (100 mg, 0.25 mmol), 0-(7-azabenzotriazol-1-yl)-/V,/V,W;/V>tetramethyluronium hexafluorophosphate (HATU) (116 mg, 0.30 mmol) and diisopropylethylamine (0.14 mL,

218

0.76 mmol) In dichloromethane (10 mL) at room température. The reaction mixture was stirred for 2 h. The mixture was partitioned between dichloromethane and water. The organic layer was dried over sodium sulfate, filtered and concentrated under reduced pressure. The crude material was purified via préparative TLC followed by chirai séparation to afford two enantiomers. Enantiomer 1 (Example 190,37 mg, 33% yield): ’H NMR (500 MHz, CDCI₃) δ 1.57-1.61 (m, 1H), 1.75-2.04 (m, 11 H), 2.62-2.64 (m, 0.5H*), 3.41-3.48 (m, 3H), 3.56-3.61 (m, 1H), 6.64 (d, 1H), 7.58 (s, 1H), 7.64 (s, 1H), 7.68 (d, 1H); Chiral HPLC rétention time 13.727 min (Method: Column: CHIRAL PAK IA 4.6 X 250 mm, 5μΜ; Mobile Phase D: 0.1% DEA In n-Hexane; Mobile Phase C: éthanol; Isocratic: 75:25; Flow Rate: 1.0 mL/min). Enantiomer 2 (Example 191,35 mg, 31% yield): ’H NMR (500 MHz, CDCI3) δ 1.57-1.62 (m, 1H), 1.76-2.05 (m, 11 H), 2.64-2.66 (m, 0.5H‘), 3.41-3.49 (m, 3H), 3.57-3.61 (m, 1H), 6.65 (d, 1H), 7.59 (s, 1H),

7.64 (s, 1 H), 7.70 (d, 1 H); Chiral HPLC rétention time 11.905 min (Method: Same as for enantiomer 1). *For both enantiomers, ’H NMR intégration indicates partial incorporation of deuterium at this position.

Examples 192 and 193: ((R)-1-(2-((fî)-1-(4-Fluoro-1H-pvrazol-1-vl)ethvl)-3H· imidazor4.5-blPvridin-5-vl)piperidin-3-vl)(pyrrolidin-1 -vDmethanone and ((R)-1 -(2-((S)-1 (4-chloro-1 H-pyrazol-1 -vl)ethvl)-3H-lmldazor4,5-b1pvridin-5-vl)piperidin-3-vl)(pyrrolidin-1 vDmethanone

To a solution of (R)-(1-(5,6-diaminopyridin-2-yl)piperidin-3-yi)(pyrrolidin-1-yl)methanone dihydrochloride (1.02 g, 2.8 mmol) in éthanol (14.0 mL) was added acetic acid (3.21 mL, 56 mmol) followed by the addition of a solution of ethyl 2-(4-fluoro-1 H-pyrazol-1yl)propanîmldate (Intermediate 59) via cannula. Triethylamine (2.34 mL) was added. The mixture was purged with nitrogen and heated to 90°C for 18 h. The solvent was removed under reduced pressure and the residue was partitioned between a saturated aqueous solution of ammonium chloride (100 mL) and dichloromethane (100 mL). The layers were separated and the aqueous layer was again extracted with dichloromethane (200 mL). The combined organic layers were washed sequentîally with a saturated

219 aqueous solution of sodium bicarbonate (200 mL) and brine (300 mL). The organic layer was then dned over sodium sulfate, filtered and concentrated under reduced pressure. The crude material was purified via flash chromatography (0-25% methanol în dîchloromethane) to afford a diastereomeric mixture of the title compounds (125 mg, 11% yield). Diastereomers were separated by préparative chiral HPLC. Diastereomer 1 (Example 192, 34 mg): Chiral HPLC rétention time 7.575 min; ’H NMR (500 MHz, CDCh) δ 1.52-1.63 (m, 1H), 1.73-1.76 (m, 1H), 1.79-1.97 (m, 6H), 1.99 (d, 3H), 2.582.67 (m, 1H), 2.66-2.94 (m, 1H), 3.00-3.08 (m, 1H), 3.39-3.51 (m, 3H), 3.53-3.61 (m,

1H), 4.21 (d, 1H), 4.46 (d, 1H), 5.64 (q, 1H), 6.64 (d, 1H), 7.41 (d, 1H), 7.45 (d, 1H),

7.75 (d, 1H); MS (ES+)(M+H) 412.3. Diastereomer 2 (Example 193, 36 mg): Chiral HPLC rétention time 8.903 min. (Chiral Method: Column: Phenomenex Kinetex C18 50 x 3.0 mm 2.6 μΜ; Gradient: Mobile Phase A: 0.1% formic acid in water; Mobile Phase B: 0.1% formic acid in methanol; Time(min)/%B: 0.00/0, 0.30/0, 3.00/100; 3.70/100, 3.71/0; 4.00/0; Flow: 1.0 mL/min); Ή NMR (500 MHz, CDCIa) δ 1.52-1.64 (m, 1H), 1.74-1.81 (m, 1H), 1.81-1.98 (m, 6H), 2.00 (d, 3H), 2.59-2.69 (m, 1H), 2.87-2.95 (m, 1H), 3.01-3.10 (m, 1H), 3.40-3.51 (m, 3H), 3.55-3.62 (m, 1H), 4.23 (d, 1H), 4.45 (d, 1H), 5.65 (q, 1H), 6.65 (d, 1H), 7.43 (d, 1H), 7.47 (d, 1H), 7.75 (d, 1H); MS (ES+)(M+H)

412.3.

Example 194: (fî)-(1-(2-( 1-(4-Methoxv-1 H-pvrazol-1-vDcvclopropvn-3H-imidazof4,5blpvridin-5-vl)piperidin-3-vl)(pyrrolidÎn-1-vDmethanone

To a solution of crude ethyi 1-(4-methoxy-1H-pyrazol-1-yl)cyclopropanecarbimidate (79.5 mg, 0.380 mmol) în éthanol (1.5 mL) was added (fî)-(1-(5,6-diaminopyridin-2yl)piperidin-3-yl)(pyrrolidin-1-yl)methanone dihydrochloride (151 mg, 0.417 mmol) followed by glacial acetic acid (0.435 mL, 7.59 mmol). The mixture was degassed with nitrogen, and then triethylamine (0.318 mL, 2.28 mmol) was added. The reaction mixture was heated at 100°C for 20 mîn and then at 85°C for 18 h. The mixture was poured into a saturated aqueous solution of ammonium chloride (40 mL) and extracted with dîchloromethane (50 mL x 3). The combined organics were washed with a

220 saturated aqueous solution of sodium bicarbonate. The aqueous layer was extracted with dichloromethane (40 mL x 2). The combined organics were dned over sodium sulfate, filtered and concentrated under reduced pressure. The crude material was purified via HPLC to afford the title compound. MS (ESI+) (M+H) 436.2; HPLC rétention time 1.85 min (Method A).

Exampie 195: (ff)-(1 -(2-(1 -(2-cvclopropyloxazol-4-vl)cvclopropvl)-3fflmidazof4.5b1pvridin-5-vl)piperidin-3-vl)(pvrrolidin-1-yl)methanone

The title compound was prepared by a method analogous to the one used for Example 160, but using 1-(2-cyc!opropyloxazo!-4-yl)cyclopropanecarboxylic acid as the starting material to afford an enantio-enriched mixture that was further purified via chiral HPLC to afford the title compound. MS (ES+) (M+H) 447.3; LCMS rétention time 2.062 min (Method N1).

Examples 196-A and 197: ((ff)-1-(2-((S)-1-(4-Chloro-1H-pvrazol-1-vl)ethvl)-3H· imÎdazoî4.5-b1pvridin-5-vl)piperidin-3-vl)(pyrrolidin-1-vl)methanone and ((/7)-1-<2-((fî)-1(4-chloro-1 H-pyrazol-1 -vl)ethvl)-3H-imidazor4.5-blPvridin-5-v0piperidin-3-vl)(pyrrolidin-1 vDmethanone ci ci

To a suspension of (/7)-(1-(5,6-diaminopyridin-2-yl)piperidin-3-yl)(pyrrolidin-1yl)methanone dihydrochloride (3.65 g, 10.1 mmol) in éthanol (15 mL) was added a solution of ethyl 2-(4-chloro-1 H-pyrazol-1-yl)propanimidate crude mixture (Intermediate

60) followed by acetic acid (11.5 mL, 201 mmol) at room température. Triethylamine (8.4 mL, 60.4 mmol) was added dropwise and the reaction mixture was stirred at 100°C for 1 h. The solvent was removed under reduced pressure and the residue was

221 partitloned between ethyi acetate (250 mL) and a saturated aqueous solution of ammonium chioride (200 mL). The aqueous layer was extracted again with ethyi acetate (200 mL). The combined organics were washed sequentially with a saturated aqueous solution of sodium bicarbonate (150 mL) and brine (150 mL), and then were dried over sodium sulfate, filtered and concentrated under reduced pressure. The crude material was purified via flash chromatography (0-5% methanol in ethyi acetate) to afford a diastereomeric mixture of the title compounds. The mixture was purified via préparative chiral HPLC (Method: Column: Phenomenex Cellulose-2 250 x 21.2 mm 5μ; Mobile phase A: Heptane; Mobile phase B: Ethanol; Gradient: Initial - 5%B, Time (min)/%B: 0.00/5,1.50/5,10.0/100,11.0/100,12.5/5; Flow rate: 28 mL/min) to afford two dlastereomers. Each diastereomer was further purified via flash chromatography (0-40% of a 20% methanol in dichloromethane solution to dichloromethane) to afford title compounds as single dlastereomers. Diastereomer 1: ((fi)-1-(2-((S)-1 -(4-Chloro-1 Hpyrazol-1-yl)ethyl)-3M-imidazo[4,5-b]pyridin-5-yl)piperidin-3-yl)(pyrrolidin-1yl)methanone (Example 196-A, 1.05 g, 24% yield): ’H NMR (500 MHz, CDCI₃) δ 1.541.66 (m, 1H), 1.75-2.00 (m, 7H), 2.04 (d, 3H), 2.59-2.70 (m, 1H), 2.89-3.00 (m, 1H), 3.08 (dd, 1H), 3.42-3.54 (m, 3H), 3.58-3.63 (m, 1H), 4.23 (br d, 1H), 4.46 (br d, 1H),

5.76 (q, 1H), 6.68 (d, 1H), 7.53 (s, 1H), 7.60 (s, 1H), 7.78 (d, 1 H); MS (ES+) (M+H) 428.3; Chiral HPLC rétention time 7.587 min (Method: Column: Phenomenex Kinetex C18 50 x 3.0 mm 2.6μ; Mobile phase A: 0.1% formic acid in water; Mobile phase B: 0.1% formic acid in methanol; Gradient: Initial - 0%B, Time (min)/%B: 0.00/0, 0.30/0, 3.00/100, 3.70/100,3.71/0, 4.00/0; Flow rate: 1.0 mL/min). Diastereomer 2: ((fi)-1-(2((fi)-1-(4-chloro-1 H-pyrazol-1-yl)ethyl)-3H-imidazo[4,5-b]pyridin-5-yl)piperidin-3yl)(pyrrolidin-1-yl)methanone (Example 197,0.84 g, 19% yield): ’H NMR (500 MHz, CDCh) δ 1.55-1.63 (m, 1 H), 1.76-1.99 (m, 7H), 2.03 (d, 3H), 2.62-2.68 (m, 1H), 2.912.96 (m, 1 H), 3.04-3.09 (m, 1H), 3.43-3.50 (m, 3H), 3.58-3.62 (m, 1H), 4.23 (br d, 1H), 4.47 (br d, 1H), 5.74 (q, 1 H). 6.66 (d, 1H), 7.52 (s, 1H), 7.60 (s, 1H), 7.78 (d, 1H); MS (ES+) (M+H) 428.3; Chiral HPLC rétention time 9.306 min (Method: same as that of Example 196-A).

222

Example 196-B: ((/7>-1-(2-((S)-1-(4-Chloro-1 H-pyrazol-l-vDethvDOH-imÎdazoM.S- blpyndin-5-vl)pipendin-3-vl)(pvrrolidin-1-vl]methanone hydrochloride ci

To a solution of Example 196-A (457 mg, 1.07 mmol) in THF (1.5 mL) was added freshly prepared 1N HCl in dioxane/THF (1:3,1.07 mL). The mixture was treated with 8 mg of ((R)-1 -(2-((S)-1 -(4-Chloro-1 H-pyrazol-1 -yl)ethyl)-3H-îmîdazo[4,5-blpyridin-5yl)piperidin-3-yl)(pyrrolidin-1-yl)methanone hydrochloride as seed crystals, and stirred at room température for 4 h. Precipitated solid material was broken up with a spatula, sonicated for ca. 30 sec, and stirred for an additional 3 h at room température. The réaction mixture was diluted with additional THF (4.5 mL) and the white, creamy homogenous suspension was stirred for 17 h at room température. The suspension was filtered, and the collected solids were washed with THF (20 mL). The filter cake was dried under reduced pressure ovemight at 40°C to afford the title compound as an offwhite solid (380 mg, 76%). Examination under a polarized light microscope shows fully crystalline material. ¹H NMR (500 MHz, CD₃OD) δ 1.60-1.68 (m. 1 H), 1.77-1.93 (m, 4H), 1.98-2.05 (m, 3H), 2.04 (d, 3H), 2.74-2.80 (m, 1 H), 3.11-3.23 (m, 2H), 3.40-3.46 (m, 2H), 3.51-3.56 (m, 1H), 3.61-3.66 (m, 1H), 4.33 (br d, 1H), 4.48 (brd, 1H), 6.04 (q, 1H), 7.14 (d, 1H), 7.58 (s, 1H), 7.91 (d, 1H), 8.00 (s, 1H); MS (ES+) (M+H) 428.3; Anal. Calculated for C₂iH26CIN7O · HCl: C, 52.29; H, 6.06; N, 20.32; Cl, 14.70. Found: C, 52.06; H, 6.09; N, 19.97; Cl, 14.66.

The absolute stereoconfigurations of Example 196-A and 197 were confirmed by the comparison of chiral HPLC rétention time between Example 196-A and 197 synthesized through the method above and ((R)-1 -(2-((/7)- 1-(4-chloro-1H-pyrazol-1-yl)ethyl)-3H· imidazo[4,5-b]pyridin-5-yl)piperidin-3-yl)(pyrrolidin-1 -yl)methanone (Example 197) synthesized by the following procedure starting from (/7)-2-(4-Chloro-1 H-pyrazol-1 yl)propanoic acid (Intermediate 68):

Step 1: (/7)-tert-Butyl 2-amino-6-(3-(pyrrolidine-1-carbonyl)piperidin-1-yl)pyridin-3ylcarbamate

Di-fert-butyl dicarbonate (327 mg, 1.50 mmol) and (fi)-(1-(5,6-diaminopyridin-2yl)piperidin-3-yl)(pyrrolidin-1-yl)methanone dihydrochloride sait (362,1.00 mmol) were suspended in tetrahydrofuran (5 mL) at room température under nitrogen. A saturated 5 aqueous solution of sodium bicarbonate (2.5 mL) was added and the reaction mixture was stirred at room température for 1.5 h. The mixture was diluted with ethyl acetate (20 mL), and then washed sequentially with water and brine. Pie organics were dried over sodium sulfate, filtered and concentrated under reduced pressure. The crude material was purified via flash chromatography (50-100% ethyl acetate in heptanes) to 10 afford (fi)-fert-butyl 2-amino-6-(3-(pyrrolidine-1-carbonyl)pîperidin-1-yl)pyridin-3ylcarbamate (295 mg, 76%). ¹H NMR (500 MHz, CDCI₃) δ 1.49 (s, 9H), 1.52-1.58 (m, 1H), 1.70-1.72 (m, 1H), 1.78-2.00 (m, 6H), 2.56-2.63 (m, 1H). 2.84 (dt, 1H), 2.94 (dd, 1H), 3.42-3.49 (m, 3H), 3.60-3.64 (m, 1H), 4.13-4.17 (m, 1H), 4.37-4.40 (m, 1H), 4.41 (brs, 1H), 5.88 (brs, 1H), 6.04 (d, 1 H), 7.20 (d, 1H); MS (ES+) (M+H) 390.4.

Step 2: fert-Butyl 2-((R)-2-(4-chloro-1H-pyrazol-1-yl)propanamido)-6-((fi)-3-(pyrrolidine1 -carbonyl)piperidin-1 -yl)pyridin-3-ylcarbamate

(R)-2-(4-Chloro-1H-pyrazol-1-yl)propanolc acid (105 mg, 0.60 mmol) and (fi)-fert-butyl 20 2-amino-6-(3-(pyrrolidine-1-carbonyl)piperidin-1 -yl)pyridin-3-ylcarbamate (195 mg, 0.50 mmol) were dissolved in ethyl acetate (0.5 mL) with pyridine (0.20 mL, 2.5 mmol) at room température. Pie mixture was cooled to 0°C. 1-Propanephosphonic acid cyclic anhydride (0.375 mL, 50% solution in ethyl acetate, 1.25 mmol) was added while stirring at 0°C. The resulting mixture was stirred for 15 min, and then warmed to room température. After stirring for 1.5 h at room température, the mixture was quenched

224

V with an aqueous solution of hydrochloric acid (0.5 M, 1 mL). The mixture was partitioned between ethyl acetate (10 mL) and water (5 mL). The organic layer was washed sequentialiy with water (5 mL), a saturated aqueous solution of sodium bicarbonate (5 mL), and brine (5 mL). The organics were dried over magnésium sulfate, filtered, and concentrated. The residue was dried under reduced pressure to afford tert-butyl 2-((R)-2-(4-chloro-1 H-pyrazol-1 -yl)propanamido)-6-((/7)-3-(pyrrolidine-1carbonyl)piperidin-1-yl)pyridin-3-y!carbamate (268 mg, 98% yield). ¹H NMR (500 MHz, CDCh) Ô 1.48 (s, 9H), 1.48-1.55 (m, 1H), 1.73-1.76 (m, 1H), 1.80-2.05 (m, 6H), 1.86 (d, 3H), 2.53-2.59 (m, 1H), 2.84-2.90 (m, 1H), 2.96-3.01 (m, 1H), 3.43-3.51 (m, 3H), 3.5510 3.60 (m, 1H), 4.12 (br d, 1H), 4.33 (brd, 1H), 5.10 (br s, 1H), 6.56 (d, 1 H), 7.30 (br s,

H), 7.57 (s, 1 H), 7.61 (s, 1 H), 7.76 (br s, 1 H), 8.58 (br s, 1 H); MS (ES+) (M+H) 546.3; Chiral HPLC rétention time 4.61 min (Method: Chiralpak AS-H 0.46 x 25 cm; Mobile Phase: CO₂/methanol; Flow: 2.5 mL/min); 97.1% de.

Step 3: ((/7)-1-(2-((/7)-1-(4-Chloro-1 H-pyrazol-1-yl)ethy!)-3H-imidazo[4,5-b]pyridin-5yl)piperidin-3-yl)(pyrrolidin-1-yl)methanone tert-Butyl 2-((R)-2-(4-ch!oro-1 H-pyrazol-1 -yl)propanamido)-6-((R)-3-(pyrrolidine-1 carbonyl)piperidin-1-yl)pyridin-3-ylcarbamate (55 mg, 0.10 mmol) was dissolved in acetic acid (0.5 mL). Methanesulfonic acid (32 pL, 0.50 mmol) was added while stîrring 20 at room température. The solution was stirred at room température for 1 h. Sodium acetate (49 mg, 0.60 mmol) was added while vortexing for 1 min. The resulting suspension was stirred for 1 h and then quenched with water (2.5 mL). The resulting mixture was extracted with ethyl acetate (5 mL x 3). The combined organics were washed sequentialiy with a saturated aqueous solution of sodium bicarbonate (10 mL x 25 2), water (10 mL) and brine (5 mL). The organics were dried over magnésium sulfate, filtered and concentrated. The residue was transferred to a flask containing dichloromethane and the mixture was concentrated to give a clear glass. The glass was dried under vacuum to afford the title compound (39.8 mg, 93% yield). ¹H NMR (500 MHz, CDCh) δ 1.54-1.62 (m, 1H), 1.76-2.05 (m, 7H), 2.02 (d, 1H), 2.62-2.66 (m, 30 1 H), 2.89-2.95 (m, 1 H), 3.03-3.08 (m, 1 H), 3.42-3.49 (m, 3H), 3.57-3.61 (m, 1 H), 4.23 (br d, 1 H), 4.46 (br d, 1 H), 5.70 (q, 1 H), 6.65 (d, 1 H), 7.51 (s, 1 H), 7.57 (s, 1 H), 7.76 (br s, 1H), 10.22 (br s, 1H); MS (ES+) (M+H) 428.3; Chiral HPLC rétention time 13.08 min (Method: Chiralpak AD-H 0.46 x 15 cm; Mobile Phase: 1.5 min 10% isopropyl alcohol/heptanes, 1.5-10 min 10% isopropyl alcohol/heptanes to 90% isopropyl

225 alcohol/heptanes, 10-18 min 90% isopropyl alcohol/heptanes; Flow: 0.4 mL/min);

98.3% de.

Examples 198 and 199: ((fii-1-(8-((S)-1-(4-Chloro-1N-pvrazol-1-vl)ethvl)-9H-purin-2vl)piperidin-3-vl)(pvrrolidin-1-yl)methanone and ((fi)-1-(8-((fî)-1-(4-chloro-1 H-pyrazol-1vlÎethvl)-9H-purin-2-vl)pÎperidin-3-vO(pyrrolidin-1-vl)me1hanone

Cl ci

Into a flask were added (fi)-(1-(5,6-diaminopyridin-2-yl)piperidin-3-yl)(pyrrolidin-1yljmethanone dihydrochioride sait (1.24 g, 3.416 mmol), éthanol (4 mL), acetic acid (3.9 mL, 68.3 mmol), and ethyl 2-(4-chloro-1H-pyrazol-1-yl)propanimidate crude réaction mixture (758 mg, 3.76 mmol, 1.1 eq. in 8 mL of éthanol). The mixture was purged with nitrogen, and then triethylamine (2.9 mL) was added. The reaction mixture was heated at 100°C for 16 h. The solvent was removed under reduced pressure and the residue was partitioned between a saturated aqueous solution of ammonium chloride (20 mL) and dichloromethane (20 mL). The aqueous layer was extracted with dichloromethane (20 mL). The combined organics were washed with a saturated aqueous solution of sodium bicarbonate (20 mL). The base wash was extracted again with dichloromethane (20 mL). The combined organics were washed with brine (20 mL), dried over sodium sulfate, filtered and concentrated under reduced pressure. The crude material was purified via flash chromatography (0-5% methanol ln dichloromethane) to afford a diastereomeric mixture of the title compounds (0.68 g, 46% yield). The mixture was purified via préparative chiral HPLC to afford two diastereomers. Diastereomer 1 (Exampie 198,189 mg, 13% yield): ¹H NMR (500 MHz, CD₃OD) δ 1.50-1.59 (m, 1 H), 1.75-2.03 (m, 10H), 2.63-2.70 (m, 1 H), 2.90-2.96 (m, 1H), 3.01 (dd, 1H), 3.42 (t, 2H), 3.49-3.54 (m, 1H), 3.68-3.72 (m, 1H), 4.77-4.83 (m, 2H), 5.72-5.77 (m, 1H), 7.50 (s, 1H), 7.86 (s, 1H), 8.58 (s, 1H); MS (ES+) (M+H) 429.2; Chiral HPLC rétention time 3.03 min (Method: Chiralcel OJ-H 10 x 250; Mobile Phase 80/20 COi/Methanol; Flow: 10.0 mL/min). Diastereomer 2 (Example 199,197 mg, 14% yield): Ή NMR (500 MHz, CD₃OD) δ 1.53-1.60 (m, 1H), 1.75-2.03 (m, 10H), 2.65-2.71 (m, 1H), 2.91-2.97 (m, 1H), 3.01 (dd, 1H), 3.43 (t, 2H), 3.50-3.55 (m, 1H), 3.70-3.74 (m,

226 φ IH), 4.78-4.84 (m, 2H), 5.75 (q, 1H), 7.50 (s, 1H), 7.87 (s, IH), 8.58 (s, IH); MS (ES+) (M+H) 429.2; Chiral HPLC rétention time 3.77 min (Method: Same as diastereomer 1).

Example 200: (/7)-(1-(2-(1-(4-Chloro-1 H-pvrazol-1-vl)cvclopropvD-3H-imidazor4.5b1pvridin-5-vDpiperidin-3-vl)(3,3-difluoroazetidin-1-vl)methanone

Cl

Step 1: (fi>Ethyl 1-(6-amino-5-nitropyridin-2-yl)piperidine-3-carboxylate

To a solution of (H)-ethyl piperidine-3-carboxylate hydrochloride sait (1.2 g, 6.21 mmol) In acetonitrile (20 mL) was added triethylamine (2.61 mL, 18.64 mmol). The mixture was stirred for 10 min at room température. 6-Chloro-3-nitropyridin-2-amine (753 mg, 10 4.31 mmol) was added and the reaction mixture was stirred at 80°C for 3 h. The mixture was cooled to room température and water was added. The mixture was extracted with ethyl acetate. The organics were dried over sodium sulfate, filtered and concentrated under reduced pressure to afford ffi>ethyl 1-(6-amino-5-nltropyridîn-2yi)piperidine-3-carboxylate (1.1 g). MS (ES+APCI) (M+H) 295.2.

Step 2: (H)-Ethyl 1-(5,6-diaminopyridin-2-yl)piperidine-3-carboxylate

A solution of (/7>ethyl 1-(6-amino-5-nitropyridin-2-yl)piperidine-3-carboxylate (1.0 g,

6.60 mmol) in éthanol (30 mL) was added to a suspension of 10% palladium-on-carbon (500 mg) in éthanol at room température. The réaction mixture was hydrogenated for 4 20 h using a balloon filled with hydrogen gas. The mixture was filtered through a pad of

Celite and the filtrate was used without further purification.

Step 3: Ethyl 1-(4-chloro-1 H-pyrazol-1-yl)cyclopropanecarbimidate hydrochloride sait Dry hydrogen chloride gas was bubbled through a solution of 1-(4-chloro-1 H-pyrazol-1 25 yl)cyclopropanecarbonitrile (1.2 g, 0.75 mmol) in éthanol (10 mL) at 0°C for 15 min. The mixture was then stirred at room température for 1 h. The solvent was removed under reduced pressure to afford ethyl 1-(4-chioro-1 H-pyrazol-1-yl)cyclopropanecarbimidate. The crude material was used without further purification.

227 • Step 4: (fi)-Ethyl 1 -(2-(1 -(4-chloro-1 H-pyrazol-1 -yl)cyclopropyl)-3/+imidazo[4,5b]pyridin-5-yl)piperidine-3-carboxylate

Acetic acid (2.5 mL, 41.66 mmol) was added to a solution of (H/ethyl 1-(5,6diaminopyridin-2-yl)piperidine-3-carboxylate, prepared in step 2, followed by ethyl 1-(45 chloro-1 H-pyrazol-1-yl)cyciopropanecarbimidate hydrochloride sait, prepared in step 3). The mixture was heated at reflux for 18 h, then cooled and concentrated under reduced pressure. The residue was partitloned between ethyl acetate and water. The organics were dried over sodium sulfate, filtered and concentrated under reduced pressure. The crude material was purified via column chromatography (100-200 silica gel, 25-70% ethyl acetate in petroleum ether) to afford (fi)-ethyl 1 -(2-(1 -(4-chloro-1 H-pyrazoi-1 yl)cyclopropyl)-3H-imidazo[4,5-b]pyridin-5-yl)piperidine-3-carboxylate (900 mg). MS (ES+)(M+H) 415.2; LCMS rétention time 2.506 min (Method N1).

Step 5: (fi)-1-(2-(1-(4-Chloro-1 H-pyrazol-1-yl)cyclopropyl)-3H-imidazo[4,5-b]pyridin-515 yl)piperidine-3-carboxylic acid. Lithium hydroxide (500 mg, 10.20 mmol) was added to a solution of (R)-ethyl 1 -(2-(1 -(4-chloro-1H-pyrazol-1-yl)cyclopropyl)-3H-imidazo[4,5b]pyridin-5-yl)piperidine-3-carboxylate (900 mg, 2.17 mmol) in tetrahydrofuran/water (1:1,10 mL) at room température. The reaction mixture was stirred for 3 h. The solvent was removed under reduced pressure and to the residue was added water. The pH of the mixture was adjusted to 6 using an aqueous solution of hydrochloric acid (1N). The mixture was extracted with ethyl acetate and the organics were dried over sodium sulfate, filtered and concentrated to afford (R)-1 -(2-(1 -(4-chloro-1 H-pyrazol-1yl)cyclopropyl)-3H-imidazo[4,5-b]pyridin-5-yl)piperidine-3-carboxylic acid (700 mg). MS (ES+)(M+H) 387.1; LCMS rétention time 1.971 min (Method N1).

Step 6: (R>(1 -(2-(1 -(4-Chioro-1 H-pyrazol-1 -yl)cyclopropyl)-3H-imidazo[4,5-b]pyridin-5yl)piperidin-3-yl)(3,3-difluoroazetidin-1-yl)methanone

3,3-Difluoroazetidine (33 mg, 0.2 mmol) was added to a mixture of (fi)-1-(2-(1-(4-chloro1 H-pyrazol-1 -yl)cyclopropyl)-3H-imidazo[4,5-b]pyridin-5-yl)piperidine-3-carboxylic acid (100 mg, 0.25 mmol), diisopropylethylamine (0.1 mL, 0.77 mmol) and O-(7azabenzotriazol-1-yl)-/V,/V,A/',/V',-tetramethyluronium hexafluorophosphate (HATU) (118 mg, 0.31 mmol) in anhydrous dichloromethane (5 mL) at room température. The reaction mixture was stirred for 2 h, then partitioned between dichloromethane and water. The organics were dried over sodium sulfate, filtered and concentrated under

228 reduced pressure. The crude material was purified via préparative TLC to afford the title compound (40 mg, 43% yield). MS (ES+) (M+H) 462.2; LCMS rétention time 2.163 minutes (Method N1). ¹H NMR (300 MHz, CDCI₃) δ 1.17-1.39 (m, 1H), 1.71-2.24 (m,

7H), 2.35-2.58 (m, 1H), 2.83-3.13 (m, 2H), 4.04-4.78 (m, 6H), 6.63 (d, 1H), 7.62 (s, 2H),

7.69-7.80 (m, 1H), 9.15 (brs, 1H).

Example 2Q1 : (ffî)-1-(2-(1-(4-Chloro-1 H-pvrazol-1-vlÎcvclopropvl)-3H-lmidazoM.5b1pvridin-5-v0piperidin-3-vl)((fi)-2-methvlpvrrolidin-1-vl)methanone

200, but using (fî)-2-methylpyrrolidine for Step 5. MS (ES+) (M+H) 454.3; LCMS rétention time 2.233 min (Method N1).

Example 202: ((fî)-1-(2-(1-(4-Chloro-1 H-pvrazol-1-vlÎcvclopropvl)-3fflmidazor4.5b]pvridin-5-v0piperidin-3-vlH(S)-2-methvÎpyrrolidin-1-vl)methanone

The title compound was prepared by a method analogous to the one used for Example 200, but using (S)-2-methylpyrrolidine for Step 5. MS (ES+) (M+H) 454.2; LCMS rétention time 2.228 min (Method N1).

229 • Example 203: (/7)-(1 -(2-(1 -(PvrazÎn-2-vl)cvclopropvl)-3H-lmldazo(4.5-blPVridÎn-5yl)piperidin-3-vl)(pyrrolidin-1-vl)nnethanone

Step 1 : (/7)-/\A(2-Amino-6-(3-(pyrrolidine-1 -carbonyl)piperidin-1 -yl)pyridin-3-yl)-1 5 (pyrazin-2-yl)cyclopropanecarboxamide

To a solution of 1-(pyrazîn-2-yl)cyclopropanecarboxylic acid (0.11 g, 0.669 mmol) in anhydrous dichloromethane (15 mL) was added 0-(7-azabenzcMazo\-'\-y\)-N_lN,N',N',tetramethyluronlum hexafluorophosphate (HATU) (0.3 g, 0.803 mmol) and diisopropylethylamine (0.35 mL, 2 mmol) at room température. The mixture was stirred 10 for 10 min and then (77>(1 -(5,6-diaminopyridin-2-yl)pîperidïn-3-yI)(pyrrolîdîn-1 yl)methanone dihydrochloride (0,29 g, 0.803 mmol) was added. The réaction mixture was stirred for 30 min. The solvent was removed under reduced pressure and the residue was partitioned between ethyl acetate and water. The organic layer was dried over sodium sulfate, filtered and concentrated under reduced pressure. The crude 15 material was purified via column chromatography (100-200 mesh silica gel, 0-5% methanol in dichloromethane) to afford (/7)-N-(2-amino-6-(3-(pyrrolidine-1carbonyl)piperidin-1 -yl)pyridin-3-yl)-1 -(pyrazin-2-yl)cyclopropanecarboxamîde (100 mg). MS (ES+APCI) (M+H) 436.1.

Step 2: (/7)-(1-(2-(1-(Pyrazln-2-yl)cyclopropyl)-3Wmidazo[4,5-b]pyridin-5-y!)piperidin-3yl)(py rrolidîn-1 -yl)methanone

To a solution of (/7)-N-(2-amino-6-(3-(pyrrolidine-1-carbonyl)piperidin-1-yl)pyridin-3-yl)1-(pyrazin-2-yl)cyclopropanecarboxamide (0.1 g, 0.22 mmol) In anhydrous methanol (0.072 mL, 1.78 mmol) was added sodium methoxide (54 mg, 1.1 mmol) and isobutanol 25 (3.5 mL). The reaction mixture was heated at 100°C for 18 h. The solvent was removed under reduced pressure and the crude material was purified via préparative TLC to afford the title compound (19 mg). MS (ES+APCI) (M+H) 418.1; LCMS rétention time 4.792 min (Method R1).

230

Example 204: (fî>(1-i2-(1-(PvrimÎdin-2-vDcvclopropvl)-3H-ÎmidazoM.5-bÎPvridin-5vl)piperidin-3-vl)(pvrrolidin-1-vDmethanone

Step 1 : (H)-( 1 -(5,6-Diaminopyridin-2-yl)plperidin-3-yl)(pyrrolidin-1 -yljmethanone To a solution of (R)-(1-(6-amino-5-nitropyridin-2-yl)piperidin-3-yl)(pyrrolidin-1yl)methanone (0.2 g, 0.62 mmol) in éthanol (10 mL) was added 10% palladium-oncarbon (200 mg). The mixture was hydrogenated at room température for 3 h using a balloon filled with hydrogen gas. The mixture was fiitered through Celite and the filtrate was used for the next step without further purification.

Step 2: (/=?>-( 1 -(2-(1 -(Pyrimidin-2-yl)cyclopropyl)-3H-imidazo[4,5-b]pyridin-5-yl)piperidin3-y I) (pyrrolidin-1 -yl)methanone

1- (Pyrimidin-2-yl)cyclopropanecarbaldehyde (0.11 g, 0.747 mmol), sulfur (40 mg, 1.24 mmol), and acetic acid (0.6 mL) were added to a solution of (fl)-(‘H5₁6-diaminopyridin-

2- yl)piperidin-3-yl)(pyrrolidin-1-yljmethanone, prepared during the previous step, in éthanol. The réaction mixture was heated to 80°C for 18 h. The solvent was removed under reduced pressure and the crude material was purified via préparative HPLC to afford the title compound (8 mg). MS (ES+APCI) (M+H) 418.2; LCMS rétention time 3.273 min (Method S1).

Example 205: (fî)-Azetldin-1-vl(1-(2-(1-(4-chloro-1 H-pyrazol-1-νΠονοΙορΓθρνΠ-3 HimidazoM.5-blpyridin-5-vhpiperidin-3-vl)methanone

Cl

Step 1: (fi)-Ethyl 1-(6-amino-5-nitropyridin-2-yl)piperidine-3-carboxylate

6-Chloro-3-nitropyridin-2-amine (1.25 g, 7.22 mmol) was added to a solution of (R)-ethyl piperidine-3-carboxylate (2.0 g, 10.32 mmol) and triethylamine (2.87 mL, 20.65 mmol) in 231 φ acetonitrile (25 mL) at room température. The reaction mixture was stirred for 3 h at 80°C, then was cooled and was partitioned between ethyl acetate and water. The organics were dried over sodium sulfate, filtered and concentrated under reduced pressure. The crude material was purified via column chromatography (100-200 mesh 5 silica gel, 0-25% ethyl acetate in petroleum ether) to afford (fî)-ethyl 1-(6-amino-5nitropyridin-2-yl)piperidine-3-carboxylate (2 g). MS (ES+APCI) (M+H) 295.0; LCMS rétention time 5.246 min (Method R1).

Step 2: (fi)-Ethyl 1-(5,6-diaminopyridin-2-yl)piperidine-3-carboxylate dihydrochloride

A solution of (R)-ethyl 1 -(6-amÎno-5-nitropyridin-2-yl)piperidine-3-carboxylate (400 mg, 1.36 mmol) in éthanol (10 mL) was added to a suspension of 10% palladium-on-carbon (200 mg) in éthanol at room température. The mixture was hydrogenated for 4 h, then was filtered through a pad of Celite and washed with éthanol. A solution of hydrogen chloride in ether was added to the filtrate to precipitate (fi)-ethyl 1-(5,6-diaminopyridin15 2-yl)piperidine-3-carboxylate dihydrochloride. MS(ES+) (M+H) 265.1404.

Step 3: (R)-Ethyl 1 -(2-(1 -(4-chloro-1 H-pyrazol-1 -yl)cyclopropyl)-3Wmidazo[4,5b]pyridin-5-y!)piperidine-3-carboxylate.

Acetic acid (0.5 mL, 8.88 mmol), ethyl 1-(4-chloro-1 H-pyrazol-120 yl)cyclopropanecarblmidate (445 mg, 1.78 mmol), a catalytic amount of sulfur, and triethylamine (0.8 mL, 5.92 mmol) were added to a suspension of (fi)-ethyl 1-(5,6diaminopyridin-2-yl)piperldine-3-carboxylate dihydrochloride (500 mg, 1.48 mmol) in éthanol (25 mL) at room température. The reaction mixture was heated at reflux for 18 h, then was concentrated under reduced pressure. The resulting residue was partitioned between ethyl acetate and water. The organic layer was dried over sodium sulfate, filtered and concentrated under reduced pressure. The crude material was purified via column chromatography (100-200 mesh silica gel, 25-70% ethyl acetate in petroleum ether) to afford (fi)-ethyl 1-(2-(1-(4-chloro-1 H-pyrazol-1-yl)cyclopropyl)-3Himidazo[4,5-b]pyridÎn-5-yl)piperidine-3-carboxylate (300 mg). MS (ES+) (M+H) 415.2.

Step 4: (fi)-1 -(2-(1 -(4-Chloro-1 H-pyrazol-1 -yl)cyclopropyl)-3H-imidazo[4,5-b]pyridin-5yl)piperidine-3-carboxylic acid

Lithium hydroxide (27.6 mg, 1.1 mmol) was added to a solution of (fi)-ethyl 1-(2-(1-(4chloro-1H-pyrazoi-1-yl)cyclopropyl)-3H-imidazo[4,5-b]pyridin-5-yl)piperidine-3232 carboxylate (300 mg, 0.77 mmol) in tetrahydrofuran/water (1:1,6 mL) at room température. The reaction mixture was stirred at room température for 3 h. The solvent was removed under reduced pressure. The residue was dissolved in water and the pH was adjusted to 6 using aqueous hydrogen chloride (1N). The mixture was extracted with ethyl acetate. The organics were dried over sodium sulfate, filtered and concentrated under reduced pressure to afford (fi)-1-(2-(1-(4-chloro-1 H-pyrazol-1 yl)cyclopropyl)-3H-imidazo[4,5-b]pyridin-5-yl)piperidine-3-carboxylic acid (200 mg). MS (ES+APCI) (M+H) 387.1; LCMS rétention time 3.432 min (Method S1).

Step 5: (fi)-Azetidin-1 -yl( 1 -(2-(1 -(4-chloro-1 H-pyrazol-1 -yl)cyclopropyl)-3H-imidazo[4,5b]pyridin-5-yl)piperidin-3-yl)methanone

Azetidine (0.1 mL, 1.42 mmol) was added to a solution of (fi)-1-(2-(1-(4-chloro-1 Hpyrazol-1 -yl)cyclopropyl)-3H-imidazo[4,5-b]pyridin-5-yl)piperidine-3-carboxylic acid (50 mg, 1.29 mmol), 0-(7-azabenzotriazol-1-yl)-N,W,W',A/',-tetramethyluronium hexafluorophosphate (HATU) (49 mg, 1.29 mmol) and diisopropylethylamine (0.1 mL,

3.ΘΘ mmol) in anhydrous dichloromethane (5 mL). The reaction mixture was stirred at room température for 2 h, then was partitioned between dichloromethane and water. The organics were dried over sodium sulfate, filtered and concentrated under reduced pressure. The crude material was purified via préparative TLC to afford the titie compound (35 mg). MS (ES+APCI) (M+H) 426.1; LCMS rétention time: 3.440 min (Method SI).

Example206: (H)-(1-i2-(1-(4-Chloro-1H-pvrazol-1-vl)cvclopropvl)-3H-Îmidazof4.5blpvridin-5-vl)piperidin-3-vl)fmorpholino)methanone

Ci

The titie compound was prepared by a method analogous to the one used for Example

205, but using morpholine for Step 5. MS (ES+APCI) (M+H) 456.1; LCMS rétention time: 3.502 minutes (Method S1).

233 φ Examples 207 and 208: ((3fî,4fî)-1-(8-(1-(1H-Pvrazol-1-vl)cvclopropvO-9H-purin-2-yl)-4meÎhvlpiperidin-3-vD(pvrrolidin-1-vl)methanone and i(3S.4S)-1-(8-f1 -i1H-pvrazol-1vl)cvclopropvl)-9H-purin-2-vl)-4-methvlpiperidin-3-vl)(pvrrolidin-1-vl)methanone

Step 1: 4-Methylpiperidine-3-carboxylic acid

4-Methylnicotinic acid (5.0 g, 36.46 mmol) in acetic acid (40 mL) was added to a wet solution of platinum oxide (500 mg) under a nitrogen atmosphère. The suspension was hydrogenated under a hydrogen atmosphère (200 PSI) at room température for 18 h. The mixture was filtered through a pad of Celite under nitrogen and the filtrate was concentrated under reduced pressure to afford 4-methylpiperidine-3-carboxylic acid (6.0 g). The material was used without further purification.

Step 2: Ethyi 4-methylpiperidine-3-carboxylate

Hydrogen chloride gas was bubbled into a solution of 4-methylpiperidine-3-carboxylic 15 acid (6.0 g, 41.90 mmol) in éthanol (150 mL) and acetic acid (30 mL) at 0°C under a nitrogen atmosphère. The reaction mixture was heated at reflux for 48 h, then was cooled and was partitioned between water and ethyi acetate. The organic layer was dried over sodium sulfate, filtered and concentrated under reduced pressure to afford ethyi 4-methylpiperidine-3-carboxylate (5.0 g). The material was used without further 20 purification.

Step 3: cis-1-tert-Butyl 3-ethyl 4-methylpiperidine-1,3-dicarboxylate

Di-tert-butyl dicarbonate (3.26 g, 15.20 mmol) was added to a solution of ethyi 4methylplperidine-3-carboxylate (2.0 g, 11.68 mmol) and triethylamine (3.9 mL, 28.05 25 mmol) in anhydrous dîchloromethane (50 mL) at 0°C. The reaction mixture was stirred at room température for 18 h. The mixture partitioned between dîchloromethane and water. The organics were dried over sodium sulfate, filtered and concentrated under reduced pressure to afford a mixture of the cis and trans diastereomers of 1-tert-butyl

3-ethyl 4-methylpiperidine-1,3-dicarboxylate (3.0 g). The crude material was purified via

234

V column chromatography (5-10% ethyl acetate in petroleum ether) to afford cis-1-tertbutyl 3-ethyl 4-methylpiperidine-1,3-dicarboxylate (2.5 g).

Step 4: cis-1 -(tert-Butoxycarbonyl)-4-methylpiperidine-3-carboxylic acid

An aqueous solution of sodium hydroxide (2N, 20 mL) was added to a solution of cis-1 teft-butyi 3-ethyi 4-methylpiperidine-1,3-dicarboxyiate (2.5 g, 9.21 mmol) in methanol (20 mL) at 0°C. The reaction mixture was stirred at 0°C for 2 h. The solvent was removed under reduced pressure and the residue was partitioned between water and ethyl acetate. The aqueous layer was acidified to pH 2 using aqueous hydrochloric acid 10 (1 N) and was extracted with ethyl acetate. The combined organics were dried over sodium sulfate, filtered and concentrated under reduced pressure to afford cis-1-(tertbutoxycarbonyl)-4-methylpiperidine-3-carboxyiicacid (2.2 g).

Step 5: cis-tert-Butyl 4-methyl-3-(pyrrolidine-1-carbonyl)piperidine-1-carboxyiate

0-(7-Azabenzotriazoi-1-yl)-/V,/V,W',/V',4etramethyluronium hexafluorophosphate (HATU) (4.12 g, 10.85 mmol), diisopropylethylamine (2.33 g, 18.08 mmol) and pyrrolidine (782 mg, 10.85 mmol) were added to a solution of cis-1 -(tert-butoxycarbonyi)-4methylpiperidine-3-carboxylic acid (2.2 g, 9.04 mmol) in anhydrous dichloromethane (30 mL) at room température. The reaction mixture was stirred at room température for 4 h.

The mixture was quenched with water and was extracted with dichloromethane. The organics were dried over sodium sulfate, filtered and concentrated under reduced pressure to afford cis-tert-butyl 4-methyl-3-(pyrroiidine-1-carbonyl)piperidine-1carboxyiate (2.6 g).

Step 6: cis-(4-Methylpiperidin-3-yl)(pyrrolidin-1-yl)methanone

Saturated ethereal hydrogen chloride (40 mL) was added to a solution of cis-tert-butyl 4* methyl-3-(pyrrolidine-1-carbonyl)piperidine-1-carboxyiate (2.6 g, 8.78 mmol) in ether (20 mL) at 0°C. The reaction mixture was stirred at room température for 2 h. The solvent was removed under reduced pressure to afford cis- (4-methylpiperidin-3-yl)(pyrrolidin-1 30 yl)methanone (1.7 g).

Step 7: cis-(1-(4-Amino-5-nitropyrimidin-2-yl)-4-methylpiperidin-3-yl)(pyrrolidin-1yl)methanone

235 φ Το a solution of cis-(4-methylpÎperidin-3-yl)(pyrrolidin-1-yl)methanone (1.7 g, 8.60 mmol)

In acetonitrile (20 mL) was added triethylamlne (3.65 mL, 26.02 mmol). The mixture was stirred for 10 min at room température. Then 2-chloro-5-nitropyrimidin-4-amine (1.19 g, 6.9 mmol) was added and the reaction mixture was stirred at 80°C for 3 h. The mixture was cooled to room température and stirred for 1 h. Ice cold water was added and the mixture was extracted with ethyi acetate. The organics were dried over sodium sulfate, filtered and concentrated under reduced pressure. The crude material was purified via column chromatography (40-100% ethyl acetate in petroleum ether) to afford cis-(1 -(4-amino-5-nitropyrimidin-2-yl)-4-methylpiperidin-3-yl)(pyrrolidin-1 10 yi)methanone (1.6 g). MS (ES+) (M+H) 335.3.

Step 8: cis-(1 -(4,5-Diaminopyrimidin-2-yl)-4-methylpiperidin-3-yl)(pyrrolidin-1 yljmethanone

A suspension of 10% palladium-on-carbon (350 mg) in éthanol was added to a solution of cis-( 1 -(4-amino-5-nîtropyrimidin-2-yl)-4-methylpiperidin-3-yi)(pyrrolidin-1 yl)methanone (600 mg, 1.79 mmol) in éthanol (20 mL) at room température under nitrogen. The mixture was hydrogenated using a balloon filled with hydrogen gas at room température for 2 h. The suspension was filtered through a pad of Celite under a nitrogen atmosphère and the filtrate was used for the next without further purification.

Step 9: ((3fî,4fi)-1 -(8-(1 -(1 H-Pyrazol-1 -yl)cyciopropyi)-9H-purin-2-yi)-4-methylpiperidin3-yi) (pyrrolidin-1 -yl)methanone and ((3S,4S)-1 -(8-(1-(1 H-pyrazol-1 -yl)cyclopropyl)-9Hpurin-2-yl)-4-methylpiperidin-3-yl) (pyrrolidin-1-yQmethanone

2-(1 H-Pyrazol-1-yl)acetonitrile (385 mg, 2.15 mmol), sulfur (60 mg) and acetic acid (1.7 mL, 28.73 mmol) were added to the filtrate containing (1 -(4,5-dtaminopyrimidin-2-yl)-4methylpiperidin-3-yl)(pyrrolidin-1-yl)methanone, prepared in the previous step. The reaction mixture was heated at reflux for 16 h. The solvent was removed under reduced pressure and the residue was partitioned between ethyl acetate and water. The organics were dried over sodium sulfate, filtered and concentrated under reduced pressure. The crude material was purified via column chromatography (100-200 mesh silica gel, 0-4% methanoi in ethyl acetate) to afford a racemic mixture of the title compounds. Enantiomers were separated by chiral HPLC. Enantiomer 1 (Example 207, 75 mg): Chiral HPLC rétention time: 7.222 min (Method: Column: CHiRALPAK IA 4.6 X 250MM, 5μΜ; Mobile Phase D: 0.1% DEA in n-Hexane; Mobile Phase: Isopropyl

236 alcohol (IPA); Isocratic 70:30; Flow: 1.0 mL/min). MS (ES+) (M+H) 421.3; LCMS rétention time: 1.978 min (Method N1). Enantiomer 2 (Example 208,78 mg): Chiral HPLC rétention time: 8.745 min (Method: Same as enantiomer 1); MS (ES+) (M+H) 421.3; LCMS rétention time: 1.966 min (Method N1).

Example 209: (/7)-(1-(8-(1-(4-Fluoro-1 H-pvrazol-1-vl)cvclopropvl)-9H-purin-2vnplperidin-3-v0(pvrrolidin-1-vnmethanone

Step 1 : (77/(1-(4,5-Diaminopyrimidin-2-yl)plperidin-3-yl)(pyrrolidin-1-yl)methanone A suspension of 10% palladium-on-carbon (100 mg) in éthanol was added to a solution of (R)-(1 -(4-amino-5-nitropyrimidin-2-yl)piperidin-3-yl)(pyrrolidin-1 -yljmethanone (200 mg, 0.62 mmol) In éthanol (10 mL) at room température under a nitrogen atmosphère. The mixture was hydrogenated using a balloon filled with hydrogen gas at room température for 2 h. The suspension was filtered through a pad of Celite under a nitrogen atmosphère and the filtrate was used for the next step without further purification.

Step 2: (/7/( 1 -(8-(1 -(4-Fluoro-1 H-pyrazol-1-yl)cyclopropyl)-9H-purin-2-yl)plperidin-3yl) (pyrrolidin-1 -yljmethanone

Ethyl 1-(4-fluoro-1 H-pyrazol-1-yl)cyclopropanecarbimidate (148 mg, 0.75 mmol) and acetic acid (0.8 mL) were added to the filtrate containing (R)-(1-(4,5-diaminopyrimidin-2yl)piperidin-3-yl)(pyrrolidin-1-yl)methanone, prepared in the previous step. The reaction mixture was heated at reflux for 16 h. The solvent was removed under reduced pressure and the residue was partitioned between ethyl acetate and water. The organics were dried over sodium sulfate, filtered and concentrated under reduced pressure. The crude material was purified via column chromatography (100-200 mesh silica gel, 0-4% methanol In ethyl acetate) to afford the title compound. MS (ES+APCI) (M+H) 425.2; LCMS rétention time: 3.545 min (Method V1).

237 φ Example 210: (fî)-( 1-(8-(1-(1 H-Pyrazol-1 -vl)cvclopropyl)-9H-purin-2-vl)piperidin-3vlÎ(pvrrolidin-1 -vltmethanone

The title compound was prepared by a method anaiogous to the one used for Example 5 209, but using ethyl 1-(1 H-pyrazol-1-yl)cyclopropanecarbimldate for Step 2. MS (ES+APCI)(M+H) 407.1 ; LCMS rétention time: 3.997 minutes (Method T1).

Example 211 : (fî)-5-(3-(Pvrrolidine-1-carbonvnpiperidin-1-vl)-AAi6(trifluoromethvl)pvridin-3-vl)-3H-imidazor4,5-blpvridine-2-carboxamide

Step 1: (fi)-(1-(5,6-Diaminopyridin-2-yl)piperidin-3-yl)(pyrrolidin-1-yl)methanone dihydrochloride

To a solution of (R)-(1-(6-amino-5-nitropyridin-2-yl)piperidin-3-yl)(pyrrolidin-1yljmethanone (4.0g) in éthanol (40 mL) was added 10% palladium-on-carbon. The mixture was stirred at room température for 16 h under a hydrogen atmosphère using a 15 balloon filled with hydrogen gas. The mixture was filtered through Celite and hydrogen chloride in 1,4-dtoxane (4N, 15 mL) was added to the filtrate. The solvent was removed under reduced pressure to afford (R)-(1-(5,6-diaminopyridin-2-yl)piperidin-3yl)(pyrrolldin-1-yljmethanone dihydrochloride which was used for the next step without further purification.

Step 2: (R)-Pyrrolidin-1 -yl(1-(2-(trichloromethyl)-3H-imldazo[4,5-b]pyridin-5-yl)piperidin-

3-yl)methanone

Formic acid (2.8 mL) and trifluoroethanol (61 mL) were added to (R)-(1-(5,6diaminopyridin-2-yl)piperidin-3-yl)(pyrrolidin-1-yljmethanone dihydrochloride. Methyl 25 2,2,2-trichloroacetimidate (1.65 mL) was added and the reaction mixture was stirred at

238

60°C for 3 h. The mixture was used for the next step without further workup or purification.

Step 3: (fi)-5-(3-(Pyrrolidine-1 -carbonyl)piperidtn-1 -yl)-AA(6-(trifluoromethyl)pyridin-35 yl)-3H-imidazo[4,5-b]pyridine-2-carboxamide

Into a vial was added 6-(trifluoromethyl)pyridin-3-amine (2.0 eq, 300 μιτιοΙ) followed by (R)-pyrrolidin-1-yl(1-(2-(trichloromethyl)-3H-imidazo[4,5-b]pyridïn-5-yl)pïperidin-3yl)methanone (815 μΙ_, 150 pmol, 0.2 M solution prepared in the previous step). The reaction mixture was shaken at 60°C for 16 h. The solvent was removed under reduced 10 pressure and the residue was partitioned between water and ethyl acetate (2 mL). The organics were concentrated under reduced pressure and the crude material was purified via HPLC to afford the title compound. MS (ES+) (M+H) 488.2; UPLC rétention time: 1.490 minutes (Method P).

Exampie 212: #7>-(1 -(2-(1 -(4-Chloro-1H-pvrazol-1-vhcvclopropvn-3fflmÎdazor4,5b1pvridin-5-vhpiperidin-3-vlÎ(2.5-dihvdro-1H-pyrrol-1-vnmethanone ci

Step 1: (fi)-Ethyl 1-(6-amino-5-nitropyridin-2-yl)piperidine-3-carboxylate 6-Chloro-3-nitropyridin-2-amine (0.8 g, 4.64 mmol) was added to a solution of (fi)-ethyl piperidine-3-carboxylate (1.0 g, 5.16 mmol) and triethylamine (1.56 g, 15.48 mmol) in acetonitrile (20 mL) at room température. The reaction mixture was stirred at 80°C for 3 h. The mixture was partitioned between ethyl acetate and water. The organics were dried over sodium sulfate and concentrated under reduced pressure. The crude material was purified via column chromatography (100-200 mesh silica gel, 10-50% ethyl acetate in petroleum ether) to afford (fi)-ethyl 1-(6-amino-5-nitropyridin-2yl)piperidine-3-carboxylate (1.0 g). MS (ES+) (M+H) 295.22.

Step 2: (R)-Ethyl 1-(5,6-diaminopyridin-2-yl)piperidine-3-carboxylate

To a solution of (fi)-ethyl 1-(6-amino-5-nitropyridin-2-yl)piperidine-3-carboxylate (1.0 g,

3.39 mmol) in éthanol (30 mL) was added a suspension of 10% palladium-on-carbon

239 (500 mg) in éthanol at room température. The mixture was hydrogenated using a balloon filled with hydrogen gas for 4 h. The mixture was filtered through a pad of Celite and the filtrate was used for the next step without further purification.

Step 3: (fi)-Ethyl 1 -(2-(1 -(4-chloro-1 H-pyrazol-1 -yl)cyclopropyl)-3H-îmidazo[4,5b]pyridÎn-5-yl)piperidine-3-carboxylate

Acetic acid (4.3 g, 67.2 mmol) was added to a solution of (fi)-ethyl 1-(5,6diaminopyridin-2-yl)piperidine-3-carboxylate (890 mg, 3.36 mmol), ethyl 1-(4-chloro-1Hpyrazol-1-yl)cyclopropanecarbimidate (445 mg, 1.78 mmol) and triethylamine (0.8 mL,

5.92 mmol) in éthanol (25 mL) at room température under a nitrogen atmosphère. The mixture was heated at reflux for 16 h, then was concentrated under reduced pressure. The resulting residue was partitioned between ethyl acetate and water. The organics were dried over sodium sulfate and concentrated under reduced pressure. The crude material was purified via column chromatography (100-200 mesh silica gel, 20-60% ethyl acetate in petroleum ether) to afford (H)-ethyl 1 -(2-(1 -(4-chloro-1 H-pyrazol-1 yl)cyclopropyl)-3H-imidazo[4,5-b]pyridin-5-yl)piperidine-3-carboxylate (600 mg). MS (ES+) (M+H) 415.2859.

Step 4: (fi)-1 -(2-(1 -(4-Chloro-1 H-pyrazol-1 -yl)cyclopropyl)-3H-imidazo[4,5-b]pyridin-520 yl)piperidine-3-carboxylic acid

Lithium hydroxide (182.4 mg, 4.32 mmol) was added to a solution of (H)-ethyl 1-(2-(1(4-chloro-1 H-pyrazol-1-yl)cyclopropyl)-3H-imÎdazo[4,5-b]pyridÎn-5-yl)piperidine-3carboxylate (600 mg, 1.44 mmol) in tetrahydrofuran:water (1:1,20 mL) at room température. The reaction mixture was stirred for 3 h. The solvent was removed under 25 reduced pressure and the resulting residue was dissolved in water. The pH of the solution was adjusted to 2 using aqueous hydrochloric acid (1N), and the producet was extracted with ethyl acetate. The organics were dried over sodium sulfate and concentrated under reduced pressure to afford (H)-1 -(2-(1 -(4-chloro-1 H-pyrazol-1 yl)cyclopropyl)-3H-imidazo[4,5-b]pyridin-5-yl)piperidine-3-carboxylic acid (500 mg). The 30 material was used for the next step without further purification. MS (ES+) (M+H) 387.3246.

Step 5: (H)-(1 -(2-(1 -(4-Chloro-1 H-pyrazol-1 -yl)cyclopropyl)-3H-imidazo[4,5-b]pyridin-5y!) pïpe ridin-3-y!) (2,5-dîhydro-1 H-pyrrol-1 -yljmethanone

240

3-Pyrroline (98.5 mg, 1.41 mmol) was added to a solution of (fi)-1 -(2-(1 -(4-chloro-1 H· pyrazol-1 -yl)cyclopropyl)-3H-lmidazo[4,5-b]pyridin-5-yl)piperidine-3-carboxylic acid (500 mg, 1.29 mmol), (^tT-azabenzotriazol-l-yO-ty/V./V^A/'.-tetramethyluronium hexafluorophosphate (HATU) (541 mg, 1.41 mmol) and diisopropylethylamine (334.7 mg, 2.58 mmol) ïn anhydrous dichloromethane (20 mL). The réaction mixture was stirred at room température for 2 h. The mixture was partitioned between dichloromethane and water. The organic layer was dried over sodium sulfate and concentrated under reduced pressure. The crude material was triturated with pentane to afford the title compound (510 mg). MS (ES+) (M+H) 438.0; LCMS rétention time 4.935 min (Method R1 ).

Example 213: (f?)-(4-(8-(1-(4-Chloro-1 Η·ρνΓ8ΖθΙ-1-νΙ)ονοΙορΓθρνΙ)-9Η·ρυι1η-2vl)morpholin-2-v0(pyrrolidin-1-vl)methanone

Cl

Step 1: (fi)-fert-Butyl2-(pyrrolidine-1-carbonyl)morpholine-4-carboxylate 0-(7-Azabenzotriazol-1 -yl)-/V,N,/V',/V',-tetramethyluronium hexafluorophosphate (HATU) (1.47 g, 4.3 mmol) was added to a solution of (fi)-4-(tert-butoxycarbonyl)morpholine-2carboxyllc acid (1 g, 4.3 mmol) and diisopropylethylamine (1.11 g, 8.6 mmol) in anhydrous dichloromethane (20 mL) at room température. Pyrrolidine (0.45 mL, 5.62 mmol) was added and the réaction mixture was stirred at room température for 2 h. The mixture was partitioned between water and dichloromethane. The organics were dried over sodium sulfate and concentrated under reduced pressure and the resulting residue was purified via column chromatography (100-200 mesh silica gel, 0-0.5% methanol In dichloromethane) to afford (fi)-te/ï-butyl 2-(pyrrolidine-1carbonyl)morpholine-4-carboxylate (0.62 g).

Step 2: (fi)-Morpho1in-2-yl(pyrrolidin-1-yl)methanone hydrochloride

A solution of hydrogen chloride in 1,4-dioxane (20 mL) was added to a solution of (fi)tert-butyl 2-(pyrrolidine-1-carbonyl)morpholîne-4-carboxylate (0.62 g, 2.1 mmol) In \ 1,4dioxane (5 mL). The reaction mixture was stirred at room température for 3 h. The

241 solvent was removed under reduced pressure and the resulting crude material was triturated with diethyl ether to afford (fi)-morpholin-2-yl(pyrrolidin-1-yl)methanone hydrochloride (0.4 g).

Step 3: (R)-(4-(4-Amino-5-nitropyrimidin-2-yl)morpholin-2-yl)(pyrrolidin-1-yl)methanone

2-Chloro-5-nitropyrimidin-4-amine (106 mg, 0.612 mmol) was added to a solution of (fi)morpholin-2-yl(pyrrolidin-1-yl)methanone hydrochloride (0.15 g, 0.66 mmol) and triethylamine (0.26 mL, 2.04 mmol) in acetonitrile (10 mL) at room température. The reaction mixture was heated at 60°C for 1 h, then was cooled to room température and stirred for 12 h. The solvent was removed under reduced pressure and the resulting crude material was triturated with diethyl ether to afford (fi)-(4-(4-amino-5nitropyrimidin-2-yl)morpholin-2-yl)(pyrrolidin-1-yl)methanone (0.11 g). MS (ES+APCI) (M+H) 323.1; LCMS rétention time: 3.518 min (Method S1).

Step 4: (fi)-(4-(4,5-Diaminopyrimidin-2-yl)morpholin-2-yl)(pyrrolidin-1-yl)methanone

To a solution of (R)-(4-(4-amino-5-nitropyrimidin-2-yl)morpholin-2-yl)(pyrrolidin-1yl)methanone (0.15 g, 0.46 mmol) in éthanol (15 mL) was added 10% palladium-oncarbon (300 mg). The mixture was hydrogenated using a balloon filled with hydrogen gas at room température for 3 h. The mixture was filtered through a pad of Celite and the filtrate was used for the next step without further purification.

Step 5: (R)-(4-(8-( 1 -(4-Chloro-1 H-pyrazol-1 -yl)cyclopropyl)-9H-purin-2-yl)morpholin-2yl)(pyrrolidin-1 -yl)methanone

Ethyi 1-(4-chloro-1H-pyrazol-1-yl)cyclopropanecarbimidate (0.13 g, 0.46 mmol), sulfur (15 mg), acetic acid (0.53 mL, 9.3 mmol) and triethylamine (0.39 mL, 2.79 mmol) were added to the filtrate containing (R)-(4-(4,5-diaminopyrimidin-2-yl)morpholin-2yl)(pyrrolidîn-1-yl)methanone, prepared in the previous step, at room température. The reaction mixture was heated to 100°C for 24 h. The solvent was removed under reduced pressure and the resulting crude material was purified via préparative TLC to afford the title compound (13 mg). MS (ES+APCI) (M+H) 443.0; LCMS rétention time 4.485 min (Method T1).

Example 214: (R)-(1-(2-(1-(2H-1 ,2.3-Triazol-2-vl)cvclopropvl)-3H-imidazof4.5-blpvridin-

5-vl)piDeridin-3-vl)(Dvrrolidin-1-vl)methanone

242

To a solution of (77)-(1-(5,6-diaminopyridin-2-yl)piperidin-3-yl)(pyrrolidin-1-yl)methanone (0.4 g, 1.36 mmol) In éthanol was added ethyl 1-(2H-1,2,3-triazol-2yl)cyclopropanecarblmidate (600 mg, crude), triethylamine (5 mL) and acetic acid (6 mL). The reaction mixture was heated to reflux for 12 h. The solvent was evaporated and the residue was dissoved in ethyl acetate. The solution was dried over sodium sulfate and concentrated. The crude material was purified via préparative TLC to afford the title compound (16 mg). MS (ES+) (M+H) 407.3; LCMS rétention time 1.793 min (Method N1).

Example 215: (77)-(1-(2-(1-(17/-lmidazol-1-vl)cvclopropyl)-3ffimldazor4.5-b1ovridin-5vl)plperidin-3-vl)(pvrrolidin-1-vl)methanone

Step 1 : (77)-(1-(5,6-Diaminopyridin-2-yl)piperidin-3-yl)(pyrrolidin-1-yl)methanone

To a solution of (77)-( 1-(6-amino-5-nitropyridin-2-yl)piperidin-3-yl)(pyrrolidin-115 yl)methanone (0.11 g, 0.34 mmol) in éthanol (15 mL) was added 10% palladium-oncarbon (210 mg). The resulting suspension was hydrogenated using a balloon filled with hydrogen gas for 3 h at room température. The mixture was filtered through a pad of Celite and the filtrate was used for the next step without further purification.

Step 2: (77)-(1-(2-(1-(177-lmidazol-1-yl)cyc1opropyl)-3H-imidazo[4,5-b]pyridin-5yl)piperidin-3-yl)(pyrrolidin-1-yl)methanone

1-(1H-lmidazol-1-yl)cyclopropanecarba1dehyde (60 mg, 0.44 mmol), sulfur (10 mg), and acetic acid (0.5 mL) were added to the filtrate containing (77)-(1-(5,6-diaminopyridin-2yl)piperidin-3-yl)(pyrrolidin-1-yl)methanone, prepared in the previous step, The reaction 25 mixture was heated at 80°C for 24 h. The solvent was removed under reduced pressure and the resulting crude material was purified via préparative TLC (10%

243 φ methanol ln dichloromethane) to afford the title compound. MS (ES+APCI) 406.1 ;

LCMS rétention time 4.739 min (Method R1).

Examples 216 and 217: ((3R,6S)-1 -(2-(1 -(4-Chloro-1 H-pyrazol-1-vl)cvclopropyl)-3H· imÎdazor4.5-blpvridin-5-vl)-6-methvlpiperidin-3-vl)(pvrrolidirt-1-vl)methanone and ((3S,6R)-1-(2-(1-(4‘Chloro-1H-pvrazol-1-vncvclopropvl)-3H-imÎdazof4,5-blpvridin-5-vl)-6methvlpÎperidin-3-vl)(pvrrolidin-1-vl)methanone

Cl ci

Step 1: cis-tert-Butyl 2-methyl-5-(pyrrolidine-1-carbonyl)piperidine-1-carboxylate 10 0-(7-Azabenzotriazol-1-yl)-/V,/V,/V’,/V’,-tetramethyluronium hexafluorophosphate (HATU) (7.50 g, 19.72 mmol), diisopropylethylamine (7.08 mL, 39.45 mmol) and pyrrolidine (1.40 g, 19.72 mmol) were added to a solution of c/s-1-(tert-butoxycarbonyl)-6methylpiperidine-3-carboxylic acid (synthesized by Μ-Boc procetion of c/s-6methylpiperidine-3-carboxylic acid, analogously prepared by the method described ln J.

Med. Chem. 2011, 54,1871 -1895.) (4.0 g, 16.44 mmol) ln anhydrous dichloromethane (40 mL) at room température. The reaction mixture was stirred at room température for 4 h. Water was added and the mixture was extracted with dichloromethane. The organics were dried over sodium sulfate and concentrated under reduced pressure to afford cis-tert-butyl 2-methyl-5-(pyrrolidine-1-carbonyl)piperidine-1-carboxylate (4.0 g).

The material was used without further purification.

Step 2: cis-(6-Methylpiperidin-3-yl)(pyrrolidin-1-yl)methanone hydrochloride

A saturated solution of hydrogen chloride ln ether (50 mL) was added to a solution of cis-tert-butyl 2-methyl-5-(pyrrolidine-1-carbonyl)piperidine-1-carboxylate (4.0 g, 13.49 25 mmol) in ether (10 mL) at 0°C. The réaction mixture was stirred at room température for 2 h. The excess ethereal hydrogen chloride was evaporatedand the residue was concentrated from diethyl ether to afford cis-(6-methylpiperidin-3-yl)(pyrrolidin-1yl)methanone hydrochloride (3.0 g). The material was used without further purification.

244 • Step 3: cis-(1-(6-Amino-5-nïtropyridin-2-yl)-6-methylpiperidin-3-yl)(pyrrolidin-1yl)methanone

Toa solution of cis- (6-methylpïperidin-3-yl)(pyrrolidin-1-yl)methanone hydrochloride (0.5 g, 2.15 mmol) in acetonitrile (10 mL) was added triethylamine (0.89 mL, 6.46 s mmol). The mixture was stirred at room température for 10 min. Then 6-chloro-3nitropyridin-2-amine (260 mg, 1.50 mmol) was added and the reaction mixture was stirred at 80°C for 3 h and then at room température for 12 h. Water was added and the mixture was extracted with ethyl acetate. The organics were dried over sodium sulfate and concentrated under reduced pressure to afford cis-(1-(6-amino-5-nitropyridin-2-yl)10 6-methylpiperidin-3-yl)(pyrrolidin-1-yl)methanone (0.5 g). MS (ES+APCI) (M+H) 334.2; LCMS rétention time 4.028 min (Method S1).

Step 4: cis-(1-(5,6-Diaminopyridin-2-yl)-6-methylpiperidin-3-yl)(pyrrolidin-1yl)methanone

A solution of cis-(1-(6-amino-5-nitropyridin-2-yl)-6-methylpiperidin-3-yl)(pyrrolidin-1 yl)methanone (500 mg, 1.50 mmol) In éthanol (20 mL) was added to a suspension of 10% palladium-on-carbon (250 mg) in éthanol under a nitrogen atmosphère. The suspension was hydrogenated using a balloon filled with hydrogen gas for 2 h at room température. The mixture was filtered through Celite and the filtrate was used for the next step without further purification.

Step 5: ((3F?,6S)-1 -(2-(1 -(4-Chloro-1 H-pyrazol-1-yl)cyclopropyl)-3H-imidazo[4,5bjpy rid in-5-y l)-6-methy Ipipe ri din-3-yl) (py rrol idin-1 -yljmethanone and ((3S,6fî)-1 -(2-(1 -(4chloro-1 H-pyrazol-1-yl)cyclopropyl)-3H-imidazo[4,5-b]pyridin-5-yl)-6-methylpiperidin-325 yl)(pyrrolidin-1-yljmethanone

Ethyl 1-(4-chloro-1/7-pyrazol-1-yl)cyclopropanecarbimidate (485 mg, 1.80 mmol) and acetic acid (1.5 mL) were added to the filtrate containing cis-(1-(5,6-diaminopyridin-2yl)-6-methylpiperidin-3-yl)(pyrrolidin-1 -yljmethanone, prepared in the previous step. The réaction mixture was stirred at 80°C for 16 h. The solvent was removed under reduced pressure and the resulting residue was partitioned between water and ethyl acetate. The organic layer was dried over sodium sulfate and concentrated under reduced pressure. The crude material was purified via column chromatography (0-4% methanol in ethyl acetate) to afford a racemic mixture of the title compounds (180 mg). The racemic mixture was further purified via chiral HPLC to afford two enantlomers.

245

Enantiomer 1 (Example 216, 65 mg): Chiral HPLC rétention time 12.120 min (Method:

CHIRAL PAK IA 4,6 X 250MM, 5μΜ; Mobile Phase D: 0.1% DEA in n-Hexane; Mobile Phase C: Ethanol; Isocratic: 80:20; Flow: 1.0 mL/min); MS (ES+APCI) (M+H) 454.1; LCMS rétention time: 3.636 min (Method S1 ). Enantiomer 2 (Example 217, 60 mg): Chiral HPLC rétention time 15.424 min (Method: same as for enantiomer 1 ); MS (ES+APCI) (M+H) 454.2; LCMS rétention time: 3.640 min (Method S1).

Examples 218 and 219: ((3F?,6S)-1-(8-(1-(4-Chloro-1H-pvrazol-1-vncvclopropvl)-9H· purin-2-vl)-6-methvlpiperidin-3-vn(pvrrolidin-1 -vDmethanone and ((33,6/7)-1 -(8-(1-(4chloro-fH-pyrazol-1-vl)cvclopropvl)-9H-purin-2-vl)-6-methvlpiperidin-3-vnipyrrolidin-1vDmethanone

Cl

The title compounds were prepared by a method analogous to the one used for Examples 216 and 217, but using 2-chloro-5-nitropyrimidin-4-amine for Step 3. Enantiomer 1 (Example 218, 28 mg): Chiral HPLC rétention time 9.672 min (Method: CHIRAL PAK IA 4.6 x 250mm, 5pm; Mobile Phase D: 0.1% DEA in n-Hexane; Mobile Phase C: Ethanol; Isocratic: 80:20; Flow: 1.0 mL/min); MS (ES+) (M+H) 455.3; LCMS rétention time: 2.318 min (Method N1). Enantiomer 2 (Example 219,18 mg): Chiral HPLC rétention time 11.149 min (Method: Same as for enantiomer 1); MS (ES+) (M+H) 455.3; LCMS rétention time: 2.315 min (Method N1).

Example 220: (fî)-AzetÎdin-1-vl(1-(2-(2-cvclopropylpvrimidin-4-vl)-3H-imidazor4.5blpvridin-5-vl)piperidin-3-vl)methanone

71. MS API-ES+ (M+H) 404; HPLC rétention time 2.447 min (Method C).

246 • Example 221 : (fî)-/V-(Cvclopropvlmethvl)-1-(2-(2-cvclopropylpvrimÎdin-4-vl)-3H^L· ÎmÎdazor4.5-b1pvridin-5-vl)-/V-methvlplperidine-3-carboxamlde

The title compound was prepared by a method analogous to the one used for Example 5 71. MS API-ES+ (M+H) 432; HPLC rétention time 2.749 min (Method C).

Example 222: (fî)-(1-(2-(1-(PvrÎdazÎn-3-vl)cvclopropvD-3ffimÎdazor4,5-b1pvridin-5vlÎpiperidin-3-vD(pvrrolîdin-1-vl)methanone

N N

Step 1 : (/7)-N-(2-Amino-6-(3-(pyrrolidine-1 -carbonyl)piperidin-1 -yl)pyridin-3-yl)-1 (pyridazin-3-yl)cyclopropanecarboxamide

To a mixture of 1-(pyridazin-3-yl)cyclopropanecarboxylic acid (160 mg, 0.97 mmol), O(7-Azabenzotriazol-1 -yl)-/V,/V,A/',/V’,-tetramethyluronium hexafluorophosphate (HATU) (445mg, 1.17 mmol) and diisopropylethyl amine (330 mg, 2.4 mmol) in anhydrous dichloromethane (10 mL) was added (/7)-(1-(5,6-diaminopyridin-2-yl)plperidin-3yl)(pyrrolidin-1-yl)methanone dihydrochloride (350 mg, 0.98 mmol) dissolved in anhydrous dichloromethane (10 mL) with diisopropylethyl amine (330 mg, 2.4 mmol). The reaction mixture was stirred at room température for 2 h, diluted with dichloromethane and washed with water. The organics were dried over sodium sulfate and concentrated under reduced pressure. The resulting crude was purified via column chromatography (100-200 silicagel mesh, 60-80% ethyl acetate In petroleum ether) to afford (/7)-N-(2-amino-6-(3-(pyrrolidine-1-carbonyl)piperidin-1 -yl)pyridin-3-yl)-1 (pyridazin-3-yl)cyclopropanecarboxamide (100 mg, 23%). MS (ES+APCI) (M+H) 436.2; LCMS rétention time: 3.390 min (Method W1).

Step 2: (/7)-(1 -(2-(1 -(Pyridazin-3-yl)cyclopropyl)-3H-imidazo[4,5-b]pyridin-5-yl)piperidin3-yl)(pyrrolidin-1-yl)methanone '

Sodium methoxide (74.4 mg, 1.37 mmol) was added to a solution of (/7)-/V-(2-amino-6(3-(py rroli dine-1 -carbonyl)piperidin-1 -yl)pyridin-3-yl)-1 -(pyridazin-3247 φ yl)cyclopropanecarboxamide (100 mg, 0.22 mmol) in isobutanol (2 mL) and methanol (2 mL). The reaction mixture was heated to 110°C for 16 h. The reaction mixture was concentrated under reduced pressure and the resulting crude wsa purified via préparative TLC to afford the titie compound (35 mg). MS (ES+APCI) (M+H) 413.2;

LCMS rétention time: 3.439 min (Method W1 ).

Example 223: (fi)-f1 -(2-(1 -(1-Methvl-1H-1.2.4-triazol-5-vl)cvclopropvlÎ-3H-Îmidazof4.5blpyridin-5-vl)piperidin-3-vlKpvrrolidin-1-vl)meÎhanone

Step 1: (fî)-(1-(5,6-Diaminopyridin-2-yl)piperidin-3-yl)(pyrrolidin-1-yl)methanone To a solution of (R)-(1-(6-amino-5-nitropyridin-2-yl)piperidin-3-yl)(pyrrolidin-1yl)methanone (0.500 g, 1.56 mmol) in éthanol (25 mL) was added palladium on carbon 10% (500 mg) suspended in éthanol. The reaction mixture was hydrogenated using a balloon filled with hydrogen gas for 3 h at room température. The reaction mixture was filtered through Celite and the filtrate was used for the next step without further purification.

Step 2: (fî)-(1 -(2-(1 -(1 -Methyl-1 H-1,2,4-triazol-5-yl)cyclopropyl)-3H-imidazo[4,5b]pyridin-5-yl)piperidin-3-yl)(pyrrolidin-1-yl)methanone (fî)-(1 -(5,6-Diaminopyridin-2-yl)piperidin-3-yl)(pyrrolidin-1 -yljmethanone, prepared during the previous step, was added to a suspension of ethyl 1-(1-methyl-1 H-1,2,4triazol-5-yl)cyclopropanecarbimïdate (0.39 g, 2.0 mmol), sulfur (20 mg, 0.31 mmol) and acetic acid (0.96 mL, 15.5 mmol) in éthanol (10 mL) at room température. The reaction mixture was heated to 80°C for 16 h. The mixture was concentrated under reduced pressure and the resulting crude was dissolved in ethyl acetate, washed with water and brine, dried over sodium sulfate and concentrated under reduced pressure. The resulting crude was purified via préparative TLC to afford the titie compound (120 mg, 18%). MS (ES+APCI) (M+H) 421.2; LCMS rétention time: 3.311 min (Method Y1).

248 φ PHARMACOLOGICAL DATA

The following protocols may of course be varied by those skilled in the art.

Génération of Human DGAT2 (hDGAT2) Construct

A construct for hDGAT2 was generated with an N-terminal FLAG tag (an octapeptide 5 with the amino acid sequence of AspTyrLysAspAspAspAspLys). For the FLAG -tagged hDGAT2 construct, the cDNA for hDGAT2 was custom-synthesized at Genscript and cloned into the pFastBacl vector (Invitrogen) by using BamHI/Xhol restriction enzymes to generate an N-terminally FLAG-tagged pFastBacl-FLAG-hDGAT2 construct (amino acids 1-388). The construct was confirmed by sequencing in both directions.

DGAT2 Expression and Préparation of the DGAT2 Membrane Fraction

Recombinant baculovirus for the FLAG-tagged hDGAT2 was generated ln SF9 insect cells using Bac-to-Bac baculovirus expression system (Invitrogen) according to the manufacturées protocol. For the expression of hDGAT2, SF9 cells (20 L) grown in Sf900ll media were infected with hDGAT2 baculovirus at a multiplicity of Infection of 1 in 15 a Wave Bioreactor System 20/50P wave bag (GE Healthcare). After 40 hours of infection, the cells were then harvested by centrifugation at 5,000 x g. The cell pellets were washed by resuspendîng in phosphate buffered saline (PBS) and collected by centrifugation at 5,000 x g. The cell paste was flash frozen In liquid N₂ and stored at -80 °C until needed. Ail operations below were at 4 °C unless otherwise noted. The cells 20 were resuspended ln lysis buffer (50 mM Tris-HCI, pH 8.0,250 mM sucrose) including 1 mM ethylenediaminetetraacetic acid (EDTA) and the complété protease inhibitor cocktail (Roche Diagnostics) at a ratio of 3 ml buffer per 1 g cell paste. The cells were lysed by dounce homogenizer. The cell débris was removed by centrifugation at 1,000 x g for 20 min, and the supematant was centrifuged at 100,000 x g for 1 hour. The 25 resulting pellet was rinsed three times by filling ultracentrifuge tubes to the top with ice cold PBS before decanting. The washed pellet was resuspended with gentle stirring for 1 hour in lysis buffer containing 8 mM 3-[(3-cholamidopropyl)dimethylammonio]-1 propanesulfonate (CHAPS) at a ratio of 1 mL buffer per 1 g of original cell paste and centrifuged again at 100,000 x g for 1 hour. The resulting supematant (hDGAT2 30 membrane fraction) was aliquotted, flash frozen in liquid N₂, and stored at -80 °C until use.

249 φ In Vitro DGAT2 Assay and Détermination of ICw> Values for DGAT2 Inhibitors

For détermination of IC50 values, the reactions were carried out in 384-well white Polyplates (Perkin Elmer) in a total volume of 20 pL. To 1 pL of compounds dissolved in 100% DMSO and spotted at the bottom of each well, 5 pL of 0.04% bovine sérum 5 albumin (BSA) (fatty acid free, Sigma Aldrich) was added and the mixture was incubated at room température for 20 minutes. To this mixture, 10 pL of hDGAT2 membrane fraction (0.01 mg/mL) diluted in 100 mM Hepes-NaOH, pH 7.4,20 mM MgCfe containing 200 nM methyl arachidonyl fluorophosphonate (Cayman Chemical; dried from ethyl acetate stock solution under argon gas and dissolved in DMSO as 5 10 mM stock) was added. After this mixture was preincubated at room température for 2 hours, DGAT2 reactions were initiated by the addition of 4 pL of substrates containing 30 pM [1-¹⁴C]decanoyl-CoA (custom-synthesized by Perkin Elmer, 50 mCi/mmol) and 125 pM 1,2-didecanoyl-sn-glycerol (Avanti Polar Lipids) dissolved in 12.5% acetone.

The reaction mixtures were incubated at room température for 40 min and the reactions 15 were stopped by addition of 5 pL of 1% H3PO4. After the addition of 45 pL MicroScint-E (Perkin-Elmer), plates were sealed with Top Seal-A covers (Perkin-Elmer) and phase partitioning of substrates and products was achieved using a HT-91100 microplate orbital shaker (Big Bear Automation, Santa Clara, CA). Plates were centrifuged at 2,000 x g for 1 min in an Allegra 6R Centrifuge (Beckman Coulter) and then were 20 sealed again with fresh covers before reading in a 1450 Microbeta Wallac Trilux

Scintillation Counter (Perkin Elmer). DGAT2 activity was measured by quantïfying the generated product [¹⁴C]tridecanoylglycerol in the upper organic phase.

Background activity obtained using 50 pM of (1 R, 2H)-2-({3’-F1uoro-4’-[(6-fluoro-1, 3benzothiazol-2-yl)amîno]-1 ,r-biphenyl-4-yl}carbonyl)cyclopentanecarboxylic acid (US 25 20040224997, Example 26) for complété inhibition of DGAT2 was subtracted from ail reactions. Inhibitors were tested at eleven different concentrations to generate IC50 values for each compound. The eleven inhibitor concentrations employed typically included 50, 15.8, 5, 1.58, 0.50, 0.16, 0.05, 0.016, 0.005, 0.0016, and 0.0005 pM. The data were plotted as percentage of inhibition versus inhibitor concentration and fit to the 30 équation, y = 100/(1 + (X/IC50)¹]. where IC50 is the inhibitor concentration at 50% inhibition and z is the Hill slope (the slope of the curve at its inflection point).

Table 13 below provides the IC50 values of the Examples for inhibition of DGAT2 in accordance with the above-described assay. Résulte are reported as géométrie

250

V meanICso values. Values in the parenthèses are géométrie mean ICso values obtained by above-desribed assay using (fi)-1-(2-((S)-1-(4-Chloro-1 H-pyrazol-1 -yl)ethyl)-3Hlmidazo[4,5-b]pyridin-5-yl)piperidin-3-yl)(pyrrolidin-1 -yl)methanone (Example 196-A) instead of 50 μΜ of (1 R, 2fi)-2-({3'-Fluoro-4'-[(6-fluoro-1,3-benzothiazol-2-yl)amino]5 1,1 '-biphenyl-4-yl)carbonyl)cyclopentanecarboxylic acid for complété inhibition of

DGAT2.

251 φ Table 13

IC50 values of Examples for Inhibition of DGAT2

Example#	DGAT2 ICso (nM)
1	1.7
2	2.71
3	4.82
4	6.64
5	8.24
6	11.5
7	16.3
8	21.1
9	22.1
10	29.2
11	35
12	54.7
13	63.6
14	72.8
15	89.6
16	102
17	118
18	196
19	208
20	221
21	346
22	357
23	432
24	731
25	297
26	1050
27	1390
28	1500
29	4370
30	1400
31	286
32	239
33	97
34	24.8
35	21.2
36	20.7
37	12.6

Example#	DGAT2 IC50 (nM)
38	7.81
39	6.85
40	6.47
41	4.56
42	3.18
43	7.41
44	36.5
45	1770
46	188
47	34.5
46	10.7
49	197
50	460
51	69.7
52	1570
53	2530
54	2750
55	1120
56	166
57	702
58	363
59	858
60	892
61	86.4
62	241
63	122
64	113
65	59.3
66	1050
67	58.2
68	54.7
69	35.4
70	162
71	1470
72	104
73	96.9(85)
74	974

Example#	DGAT2 IC50 (nM)
75	764
76	227
77	107
78	83.8
79	230(238)
80	1400
81	204
82	165
83	128
84	9.55
85	1160
86	288
87	102
88	190
89	546
90	315
91	312
92	242
93	202
94	107
95	18.2
96	37.3
97	Θ6.Θ
98	94.4
99	72.5
100	47.8
101	60.5
102	99.6
103	73.7
104	190
105	28.9
106	5.68
107	153
108	28.7(26.2)
109-A	13.8
109-B	20.2(15)
110	41.5

252

Example#	DGAT2 IC50 (ηΜ)
111	697
112	9.23
113	137
114	90.3
115	67.9
116	66.2
117	409
118	301
119	22.2
120	21.5
121	14.3
122	10.9
123	276
124	5.27
125	54
126	3470
127	235
128	7.16
129	4540
130	1900
131	34.4
132	695
133	252
134	210
135	4240
136	6.32
137	1280
138	71.6
139	62.3
140	207
141	354(301)
142	24.8
143	511
145	411
146	401
147	9.21
148	187

Example#	DGAT2 IC50 (ηΜ)
149	766
150	47.3
152	285
153	48.1
154	40.5
155	35.6
156	275
157	211
158	3.58
159	28.2
160	789
161	218
162	434
163	3400
164	2800
165	16500
166	2280
168	1390
169	292
171	161
172	83
173	1250
174	1170
175	91.3
176	73.4
177	953
179	512
180	434
181	17400
182	6.03
183	37.5
184	15.2
185	231
186	162
187	10.5
188	31.4
189	34.1

Example#	DGAT2 IC50 (nM)
190	35.9
191	6600
192	88.6
193	128
194	101
195	142
196-A	14.5(20.6)
196-B	38.5
197	33.7
198	43
199	18.9(32.9)
200	74.2
201	91
202	321
203	176
204	656
205	188
206	246
207	702
208	37400
209	101
210	342
211	845
212	18.9
213	161
214	396
215	483
216	9200
217	131
218	2310
219	82.8
220	270
221	290
222	531
223	1370

253

Détermination of ICsn values for DGAT2 inhibitors in human hepatocvtes

For évaluation of the effects of DGAT2 inhibitors in a cell-based setting, cryopreserved human hépatocytes (Lot QOC, Celsis, Chicago, IL) were thawed and plated onto type I collagen-coated plates according to the manufactureras Instructions. After 24 hours overnight recovery period, the cells were overlayed with media containing 250 pg/ml Matrigel (BD Biosciences, San José, CA). The following day, media was aspirated and replaced with serum-free Williams Media E (Life Technologies, Grand Island, NY) containing 400 μΜ sodium dodecanoate (Sigma-Aldrich, St. Louis, MO). Forty minutes iater, DGAT2 inhibitors (prepared as 100X stocks in 25% DMSO, 75% Williams’ Media 10 E) were added to the desired final concentration. Ail wells contained a sélective DGAT1

Inhibitor (Example 3, W02009016462) at a concentration (3 μΜ) that completely suppressed endogenous DGAT1 activity. After a 20 minute preincubation, 0.2 μΟΐ [1,3¹⁴C]-glycerol (American Radio Chemicals, St. Louis, MO) was added to each well and mixed by gentle plpetting prior to a 3 hour incubation. At this point, media was aspirated 15 and the cells were lysed in isopropyl alcohol: tetrahydrofuran (9:1 ) prior to centrifugation at 3000 rpm for 5 minutes. Radiolabeled lipids were resolved using a 2-solvent system by thin layer chromatography using standard technique (solvent 1 contained ethyl acetate: isopropyl alcohol: chloroform: methanol: 0.25% potassium chloride in water (100:100:100:40.2:36.1, v/v/v/v) and solvent 2 contained hexane: diethyl ether: acetic 20 acid (70:27:3, v/v/v)). After séparation, radiolabeled lipids were visualized using a

Molecular Dynamics’ Phosphorlmager system. The half maximal inhibitory concentrations (IC50 values) were determined using GraphPad Prism (GraphPad Software, Inc., La Jolla, CA).

Table 14 below provldes IC₅₀ values for the Examples in accordance with the above25 described assay. Results were reported as average IC₅₀ values, low and high IC50 range (95% confidence interval).

254

Φ Table 14

ICso values of selected DGAT2 inhibitors in primary human heptocyte.

Example #	IC₅₀ (nM)
58	86.8
73	7.9
76	25.1
78	9.4
81	47.7
82	15.8
95	15.9
108	2.8
109-A	2.1
109-B	5.5
141	10.7
175	71.7
196-A	4.6
197	5.5
198	7.8
199	2.5
209	8.9

Acute effects of DGAT2 inhibitors on plasma TAG levels.

Blockade of hepatic DGAT2 activity has been shown to inhibit the sécrétion of

VLDL TAG (18). To evaluate the acute effects of DGAT2 inhibitors on hepatic TAG production, male Sprague Dawley rats (-200 g, Harlan Laboratories Inc.) were fed a low fat, high-sucrose diet (TD03045, Harlan Laboratories Inc.) for 2 days prior to dosing with DGAT2 inhibitors. At this time, animais were fasted for 4 hours and compounds administered as a solution in 0.5% methylcellulose. Two hours after treatment with DGAT2 inhibitors, blood was drawn from the latéral tail vein and plasma TAG levels determined using a Roche Hitachi Chemistry analyzer according to the manufacturées instructions. Data were analyzed using GraphPad Prism (GraphPad Software, Inc., La

255

Jolla, CA) and are shown as a box-an-whiskers plot with the whiskers defining the first and 99^ percentile. Statistical analysis was performed using one-way ANOVA followed by Dunnett’s multiple comparison test. * p<0.05, **p<0.001, *“p<0.0001.

Figure 4 provides acute effects of DGAT2 inhibitors on plasma TAG levels in Sprague 5 Dawley rats for the Examples 95,108 and 109-A in accordance with the abovedescribed method.

Powder X-ray Diffraction

Powder diffraction analysis was conducted using a Broker D8 diffractometer equipped with a Cu radiation source, fixed slits (divergence=1.0 mm, anti-scatter=0.6 10 mm, and receiving=0.6 mm) and a scintillation counter detector. Data was collected in the Theta-Theta goniometer at the Cu wavelength Ken =1.54056 Â from 3.0 to 40.0 degrees 2-Theta using a step size of 0.040 degrees and a step time of 2.0 second. Xray tube voltage and amperage were set at 40 kV and 40 mA respectively. Samples were prepared by placement in a Nickel Disk (Gasser & Sons, Inc. Commack, NY) and 15 rotated during data collection. Data were collected and analyzed using Broker

DIFFRAC Plus software (Version 2.6). A X-ray powder diffraction pattern and total Peak list for Crystalline Form 1 of Example 109-B are shown in Table 15. A X-ray powder diffraction pattern and total Peak list for Crystalline Form 1 of Example 109-C are shown in Table 16. In addition, a X-ray powder diffraction pattern and Total Peak list for

Crystalline Form 1 of Example 196-B are shown in Table 17. Peaks with relative intensîty of à 7% were generally chosen. The peaks which were not resolved or were consistent with noise were also discarded. The powder X-ray diffraction values are generally accurate to within ± 0.2 2-Theta degrees, due to slight variations of instrument and test conditions.

256

Table 15. X-ray powder diffraction pattern: Total Peak list for Crystalline Form 1 of Example 109-B.

Angle	Intensity %
2-Theta⁰	%
5.8	94
7.9	18
9.5	7
10.4	7
10.7	16
12.2	10
12.8	32
14.3	10
15.8	20
17.0	17
17.4	15
18.2	30
18.6	21
19.8	100
20.5	39
21.4	15
21.8	25
22.6	82
23.3	33
23.9	27

257

Angle	Intensity %
2-Theta °	%
24.5	12
25.5	19
25.7	26
26.6	18

Table 16. X-ray powder diffraction pattern: Total Peak list for Crystalline Form 1 of Example 109-C.

Angle	Intensity %
2-Theta °	%
7.8	86
8.8	15
9.7	13
10.0	19
10.6	47
11.9	63
13.3	28
15.2	100
15.6	44
16.7	56
17.6	27
18.0	43

258

Angle	Intensity %
2-Theta °	%
18.2	33
18.6	15
19.5	66
20.0	18
20.2	40
20.7	29
20.9	40
21.1	29
21.5	52
22.1	40
22.6	54
22.7	35
23.4	67
23.9	17
24.7	53
25.0	21
25.2	48
25.6	16
26.1	33
26.6	42
26.8	31
27.2	23
28.0	15

259

Angle	Intensity %
2-Theta °	%
28.7	20
29.1	17
29.6	19
30.1	28
30.8	30
31.4	13
31.9	23
32.4	20
32.7	15
33.3	19
33.9	25
34.6	13
35.3	16
35.7	16

260

Table 17. X-ray powder diffraction pattern: Total Peak list for Crystalline Form 1 of

Example 196-B.

Angle	Intensity %
2-Theta °	%
7.9	100
9.0	12
9.8	16
10.7	42
11.8	40
13.4	22
15.3	60
15.6	62
16.7	26
17.1	30
17.8	34
18.0	27
18.3	31
19.6	66
20.3	25
20.6	56
21.0	35
21.7	30
22.0	57
22.2	47

261

Angle	Intensity %
2-Theta °	%
22.5	56
23.2	41
23.6	79
24.7	29
25.3	41
25.6	54
26.3	27
27.2	44
27.6	25
28.6	26
29.5	22
30.3	19
30.9	30
31.7	32
32.3	21
34.1	25
34.5	22

Solid state NMR:

A sample of the compound of Example 109-B was tightly packed into a 4 mm ZrO₂ rotor. Spectra were collected at room température and pressure on Varian 4 mm CPMAS probe positioned into a Varian Unity Inova 400 MHz (’H frequency) NMR spectrometer. The packed rotor was oriented at the magic angle and spun at 12.0 kHz. The ’³C solid state spectrum was collected using a proton decoupled cross-polarization magic angle spinning experiment (CPMAS). The cross-polarization contact time was 262 set to 5.0 ms. A proton decoupling field of approximately 95 kHz was applied. 2048 scans were collected with recycle delay of 5 seconds. The carbon spectrum was referenced using an extemal standard of crystalline glycine, setting its upfield résonance to 176.5 ppm. The chemical shift data is dépendent on the testing conditions (i.e. spinning speed and sample holder), reference material, and data processing parameters, among other factors. Typically, the solid state NMR (ss-NMR) results are accurate to within about ± 0.2 ppm.

Table 18. ss-NMR diffraction pattern: Total Peak list for Crystalline Form 1 of Example 10 109-B.

^ldC Chemical Shifts [ppm]^a
20.7
23.0
23.6
25.4
25.8
26.2
27.1
28.4
28.9
39.4
40.5
40.8
41.2

263

¹³C Chemical Shifts [ppm]^a
41.5
41.9
45.7
46.6
47.6
103.7
107.0
111.4
115.3
119.0
125.0
127.0
130.7
132.1
138.8
142.8
143.6
145.1
148.1
152.2
156.0
158.5

264

¹³C Chemical Shifts [ppmf

170.3

173.2 (a) Referenced to extemal sample of solid phase glycine at 176.5 ppm.

Throughout this application, various publications are referenced. The disclosures of these publications in their entireties are hereby incorporated by reference into this application for ail purposes.

It will be apparent to those skilled in the art that various modifications and variations can be made in the présent invention without departing from the scope or io spîrit of the invention. Other embodiments of the invention will be apparent to those skilled in the art from considération of the spécification and practice of the invention disclosed herein. It is intended that the spécification and examples be considered as exemplary only, with a true scope and spîrit of the invention being indicated by the following daims.

Claims

What is claimed is:

1. A compound of Formula (i) wherein:

A is CR^eR⁷, O or S; B is a bond, oxetanyl, wherein m is 0, 1 or 2; p is 1,2, 3 or 4;

C and D are each individually selected from N, CH, CF and C(CH3), wherein only one of C and D Is N;

R¹ is -C(O)-heterocyclyl, -C(O)-NR⁴R⁵, or a heteroaryl, wherein said heterocyclyl or heteroaryl is optionally substituted with 1 or 2 substituents selected independently from (Ci-C4)alkyl, (C₃-C6)cycloalkyl, (C_rC₄)alkoxy, (C3-C_e)cycloalkoxy, halo, hydroxy(Ci-C4)alkyl, mono-N- or di-N,N-(C_rC4)alkylamino, mono-N- or di-N,N-(C₃C₆)cycloalkylamino, heterocyclyl, hydroxyl and cyano;

R² is (Ci-C4)alkyl, (CrC4)alkoxy, (C3-Ce)cycloalkyl, (C3-Ce)cycloalkoxy, aryl, aryloxy, heteroaryloxy, heteroaryl, heterocyclyl, aralkyl, heteroaralkyl, -C(O)-heterocyclyl, C(O)-NR⁴R⁵, or -NR⁴-C(O)-R⁵, wherein alkyl, alkoxy, cycloalkyl, cycloalkoxy, aralkyl, heteroaralkyl, aryl, aryloxy, heteroaryloxy, heteroaryl, heterocyclyl are each optionally substituted with one, two or three substituents selected independently from (CrC4)alkyl, (C_rC4)alkoxy, (C₃-C_e)cycloalkyl, (C₃-C₆)cycloalkoxy, -C(O)-(Cr C₄)alkyl, -C(O)-(C3-Ce)cycloalkyl, halo, -C(O)-(C_rC4)alkoxy, -C(O)-(C₃C₆)cycloalkoxy, mono-N- or di-N,N-(Ci-C4)a!kylamino, mono-N- ordi-N,N-(C₃C_e)cycloalkylamino, (Ci-C4)alkylcarbonylamino, (C3-C₆)cycloalkylcarbonylamino, (Ci-C4)alkylcarbonyl-N-(Ci-C₄)alkylamino, (CrC4)alkylcarbonyl-N-(C₃266 φ Ce)cycloalkylamino, (C₃-Ce)cycloalkylcarbonyl-N-(Ci-C4)alkylamino, (C₃C6)cycloalkylcarbonyl-N-(C₃-C6)cycloalkylamino, aminocarbonyl, mono-N- or di-Ν,Ν(CrC4)alkylamînocarbonyl₍ mono-N- or di-N,N-(C₃-Ce)cycloaminocarbonyl, mono-Nor di-N,N-(Ci-C4)alkylcarbonyl, mono-N- or di-N.N-ÎCrCeîcycloalkylcarbonyl, mono5 N- or di-N,N-(CrC4)alkoxycarbonyl₍ mono-N- or di-N,N-(C₃-C6)cycloalkoxycarbonyl, (C₁-C4)alkylthio, (C3-C_e)cycloalkylthio, aminosulfonyl, (Ci-C4)alkylsulfinyl, (C_rC₄)alkylsulfonyl, (QrCeJcycloalkylsulfinyl, (C₃-Ce)cycloalkylsulfonyl_t mono-N- or diN,N-(Ci-C4)alkylaminosulfonyl, mono-N- or di-N,N-(C3-C6)cycloalkylaminosulfonyl, (Ci-C4)alkylsulfonylamino, (CæCeîcycloalkylsulfonylamino, (Ci-C4)alkylsulfonyl-N10 (Ci-C4)alkylamino, (Ci-C4)alkylsulfonyl-N-(C₃-Ce)cycloalkylamino, (C₃Ce)cycloalkylsulfonyl-N-(Ci-C4)alkylamino₍ (C3-C₆)cycloalkylsulfonyl-N-(C₃Cejcycloalkylamino, aryl, heteroaryl, heterocyclyl, oxo, carboxyl, amino, hydroxyl and cyano, wherein said alkyl, cycloalkyl, aryl, heteroaryl, heterocyclyl, alkoxy and cycloalkoxy are optionally substituted independently with one to nine fluoro, or 1,2 15 or 3 substituents selected from halo, -C(O)-OH, -C(O)-(C_rC4)alkoxy, aminocarbonyl, mono-N- or di-N,N-(CrC4)alkylcarbonyl, mono-N- or di-N,N-(C₃Ce)cycloalkylcarbonyl, cyano, amino and hydroxyl;

R³ is (Ct-C4)alkyl, (QrCeJcycloalkyl, hydroxyl or fluoro, wherein said alkyl is optionally substituted with one to nine fluoros and said (C₃-Ce)cycloalkyl is optionally 20 substituted with one to six fluoros;

R⁴ and R⁵ are each independently selected from hydrogen, (Ci-C4)alkyl, (C3C6)cycloalkyl, aryl, heteroaryl, aryloxy, heteroaryloxy, aralkyl, heteroaralkyl, heterocyclyl, (CrC4)alkoxy, and (C3-Ce)cycloalkoxy, wherein R⁴ and R⁵ are each optionally substituted with (Ci-C4)alkyl, (Ci-C4)alkoxy, (C3-C₆)cycloalkyl, (C₃25 Ce)cycloalkoxy, halo or cyano, wherein each of said alkyl, cycloalkyl, alkoxy or cycloalkoxy is optionally substituted with one to nine fluoros;

R® and R⁷ are each independently hydrogen, (Ci-C4)alkyl, fluoro, (CrC₄)alkoxy, hydroxyl or cyano, wherein said alkyl is optionally substituted with one to nine fluoros;

30 R⁸ is selected from fluoro, methyl or trifluoromethyl;

R⁹ and R¹⁰ are each independently selected from hydrogen, fluoro, (Ci-C₄)alkyl, (C₃Cejcycloalkyl, aryl or heteroaryl, wherein said alkyl is optionally substituted with one to nine fluoros, and said cycloalkyl is optionally substituted with one to six fluoros, and said aryl and heteroaryl are optionally substituted with 1,2 or 3 substituents

267 independently selected from fluoro, chloro, methyl, ethyl, Isopropyl, cyclopropyl, methylthio, methoxy, cyano, tnfluoromethyl, difluoromethyl, trifluoromethoxy, difluoromethoxy, oxo and trifluoromethylthio; and n is 0,1 or 2;

or a tautomer thereof or a pharmaceutically acceptable sait of said compound or tautomer.
2. The compound of claim 1 wherein R¹ is -C(O)-heterocyclyl, -C(O)-NR⁴R⁵, pyridyl,

6,7-dihydro-5H-pyrrolo[2,1-c][1,2,4]triazolyl, 6,7-dihydro-5H-pyrrolo[1,2cjimidazolyl, wherein R¹ is optionally substituted with 1 or 2 substituents independently selected from fluoro, methyl, hydroxyl or -CH₂OH;

Dis CH, N, or CF;

B is a bond, oxetanyl or wherein p is 1 or 2;

R³ is fluoro or methyl;

R⁶ and R⁷ are each Independently hydrogen, fluoro or methyl;

R⁸ Is selected from fluoro or methyl; and

R⁹ and R¹⁰ are each individually selected from hydrogen, fluoro, or methyl;

or a tautomer thereof or a pharmaceutically acceptable sait of said compound or tautomer.
3. The compound of claim 2 wherein R¹ is -C(O)-heterocyclyl or -C(O)-NR⁴R⁵wherein said heterocyclyl is optionally substituted with 1 or 2 substituents independently selected from fluoro and methyl;

B is a bond, wherein p is1 or 2;

R² is selected from phenyl, furyl, thîenyl, oxazolyl, thiazolyl, imidazolyl, pyrazolyl,

1,2,3-triazolyl, 1,2,4-triazolyl, tetrazolyl, isoxazolyl, isothiazolyl, oxadiazolyl, thiadiazolyl, pyridinyl, pyridiazinyl, pyrimidinyl, pyrazinyl, pyridin-2(1 H)-onyl,

26Θ φ pyridazin-2( 1 H)-onyl, pyrimidin-2(1 H)-onyl, pyrazin-2(1 Ffl-onyl, imidazo[1,2- ajpyridinyl, pyrazolo[1,5-a]pyridinyl, 2H-benzo[b][1,4]oxazin-3(4F0-onyl, 2,3dihydro-1 H-indenyl, 1,2,3,4-tetrahydronaphthalenyl, 5,6,7,8-tetrahydroisoquinolinyl, 5,6,7,8-tetrahydroquinolinyl, 6,7-dihydro-5H-cyclopenta[b]pyridinyl, 6,7-dihydro-5H5 cyclopenta[c]pyridinyl, 1,4,5,6-tetrahydrocyclopentatcJpyrazolyl, 2,4,5,6tetrahydrocyclopentafcjpyrazolyl, 5,6-dihydro-4H-pyrrolo[1,2-bJpyrazolyl, 6,7dihydro-5H-pyrrolo[1,2-b][1,2,4]triazolyl, 5,6,7,8-tetrahydro-[1,2,4]triazolo[1,5ajpyridinyl, 4,5,6,7-tetrahydropyrazolo[1,5-a]pyridinyl, 4,5,6,7-tetrahydro-l Hindazolyl, 4,5,6₁7-tetrahydro-2H-indazolyl, phenyloxy, pyridinyloxy, benzyl, îo pyridinyl-(CH₂)-, pyrazolyl-(CH₂)-, cyclopropyl, and cyclobutyl; wherein said R² is optionally substituted with 1, 2 or 3 substituents independently selected from (CiC₄)alkyl, (C1-C4) alkoxy, (Cg-Cejcycloalkoxy, cyclopropyl, halo, hydroxyl, amino, dimethylamino, methylamino, cyclopropylamino, aminocarbonyl, methylaminocarbonyl, (CrC4)alkyIthio, (C₃-C₆)cycloalkylthio, aminosulfonyl,

15 methylaminosulfonyl, phenyl, and heteroaryl wherein heteroaryl Is selected from furyl, thienyl, oxazolyl, thiazolyl, Imidazolyl, pyrazolyl, triazolyl, isoxazolyl, isothiazolyl, oxadiazolyl, thiadiazolyl, pyridinyl, pyridiazinyl, pyrimldinyl, pyrazinyl, pyridin-2(1 /-/)-onyl, pyridazin-2(1 H)-onyl, pyrimidin-2(1^-only, pyrazin-2(1H)-onyl, oxetanyl, azetidinyl, and pyrrolidinyl, wherein said alkyl, cyclopropyl, azetidinyl,

20 pyrrolidinyl, alkoxy and cycloalkoxy are optionally substituted with oxo, cyano, or up to three fluoro or hydroxyl, and said phenyl or heteroaryl is optionally substituted independently with up to three groups selected from halo, methyl, trifluoromethyl, difluoromethyl, methoxy, trifluoromethoxy, difluoromethoxy, cyano, cyclopropryl, methylthio, oxo and trifluoromethylthio; and

25 R⁹ and R¹⁰ are each individually selected from hydrogen and methyl·, or a tautomer thereof or a pharmaceutically acceptable sait of said compound or tautomer.
4. The compound of claim 3 wherein R¹ is -C(O)-heterocyclyl or -C(O)-NR⁴R⁵

30 wherein said heterocyclyi is selected from pyrrolidinyl, 2,5-dihydro-1 H-pyrrolyl, azetidinyl, piperidinyl and morpholinyl and said heterocyclyi is optionally substituted with 1 or 2 substituents independently selected from fluoro and methyl;

C is CH, N or CF;

A isCH₂orO;

269

R^zis phenyl, furyl, thienyl, oxazolyl, thiazolyl, Imidazolyl, pyrazolyl, triazolyl, tetrazolyl, isoxazolyl, isothiazolyl, oxadiazolyl, thiadiazolyl, pyridinyl, pyridîazinyl, pyrimidinyl, pyrazinyl, pyridin-2(1H)-onyl, pyridazin-2(1 H)-onyl, pyrimidin-2(1H)-onyl, pyrazin-2(1 H)-onyl, phenyloxy, pyridinyloxy, benzyl, pyridinyl-(CH2)- or pyrazolyl-(CH2)-, wherein R² is optionally substituted with 1,2 or 3 substituents independently selected from fluoro, chloro, methyl, ethyl, isopropyl, cyclopropyl, hydroxyl, amino, methylthio, methoxy, cyano, trifluoromethyl, difluoromethyl, trifluoromethoxy, difluoromethoxy, oxo and trifluoromethylthio;

R⁴is hydrogen or methyl;

R⁵ is hydrogen or methyl;

or a tautomer thereof or a pharmaceutically acceptable sait of said compound or tautomer.
5. The compound of claim 4 wherein R¹ is -C(O)-heterocyclyl, wherein said heterocyclyl is selected from pyrrolidinyl, 2,5-dihydro-1/-Apyrrolyl, 3,3difluoroazetidinyl, 3,3-difluoropyrrolidinyl and morpholinyl;

Bis wherein p Is 1 or 2; and

R² is phenyl, furyl, thienyl, oxazolyl, thiazolyl, imidazolyl, pyrazolyl, triazolyl, Isoxazolyl, isothiazolyl, oxadiazolyl, thiadiazolyl, pyridinyl, pyridîazinyl, pyrimidinyl, pyrazinyl, phenyloxy, pyridinyloxy, benzyl, pyridinyl-(CH₂)-, or pyrazolyl-fChfe)-; wherein R²is optionally substituted with 1,2 or 3 substituents selected from independently fluoro, chloro, methyl, ethyl, isopropyl, cyclopropyl, methylthio, methoxy, cyano, trifluoromethyl, difluoromethyl, trifluoromethoxy, difluoromethoxy, oxo and trifluoromethylthio;

or a tautomer thereof or a pharmaceutically acceptable sait of said compound or tautomer.
6. The compound of claim 5 wherein

R² is Mlinked pyrazolyl optionally substituted with 1, 2 or 3 substituents independently selected from fluoro, chloro, methyl, ethyl, isopropyl, cyclopropyl,

270 hydroxyl, amino, methylthio, methoxy, cyano, trifluoromethyl, difluoromethyl, trifluoromethoxy, difluoromethoxy and trifluoromethylthlo or a tautomer thereof or a pharmaceutically acceptable sait of said compound or tautomer.
7. The compound of claim 6 wherein R’ is

C Is CH or CF;

A Is CH₂;

n Is 0; and

R² îs N-linked pyrazolyl substituted at the 4 position with fiuoro or chloro; or a tautomer thereof or a pharmaceutically acceptable sait of said compound or tautomer.
8. The compound:

(1 -(2-(1 -(4-f luoro-1 H-pyrazol-1 -yl)cyclopropyl)-3H-imldazo[4,5-b]pyridin-5yl)piperidin-3-yl)(pyrroiidin-1-yl)methanone;

(1 -(8-(1 -(4-chloro-1 H-pyrazol-1 -yl)cyclopropyl)-9H-purin-2-yl)piperidin-3yl)(pyrrolidin-1-yl)methanone;

(1 -(2-(1 -(4-chloro-1 H-pyrazol-1 -yl)cyclopropyl)-3H-imidazo[4,5-b]pyridin-5yl)plperidin-3-yl)(pyrrolidin-1-yl)methanone;

(1 -(2-(2-(4-chloro-1 H-pyrazol-1 -yi)propan-2-yl)-3H-imldazo[4,5-b]pyridîn-5yl)piperidin-3-yl)(pyrrolidin-1-yl)methanone;

( 1 -{2-[1 -(4-chloro-1 H-pyrazol-1 -yi)cyclopropyl]-3/-Aimidazo[4,5-b]pyridin-5-yi}(2,2,6,6²H₄)piperidin-3-yl](pyrrolidin-1-yl)methanone;

( 1 -(2-((fî)-1-(4-fluoro-1H-pyrazol-1-yl)ethyl)-3/7-imidazo[4,5-b]pyridin-5-yl)piperidin-3yi)(pyrrolidin-1-yi)methanone;

(1 -(2-((S)-1 -(4-f luoro-1 H-pyrazol-1 -yl)ethyl)-3H-imidazo[4,5-b]pyridin-5-yl)piperidin-3yl)(pyrrolidin-1 -yl)methanone;

( 1 - (2-( ( S)-1 -(4-chloro-1 H-pyrazol-1 -yl)ethyl)-3H-imidazo[4,5-b]pyridîn-5-yl)piperidin-

3-yl)(pyrroiidin-1 -yl)methanone;

(1-(2-((fî)-1-(4-chloro-1FFpyrazol-1-yl)ethyl)-3H-imidazo[4,5-b]pyridin-5-yl)piperidin3-yl)(pyrrolidin-1 -yl)methanone;

271
9 (1 -(8-((S)-1 -(4-chloro-1 H-pyrazol-1 -yl)ethy I )-9 W-purin-2-yl) ρΐρβ ridin-3-y I) (py rrolîdïn-1 yljmethanone;

(1 -(8-(( R)-1 -(4-chloro-1 H-pyrazol-1 -yl) ethyl)-9 H-pu ri n-2-yl) piperid i n-3-y I) (pyrrol idin -1 yljmethanone; or

5 (1-(8-(1-(4-fluoro-1 H-pyrazol-1 -yl)cyclopropyl)-9H-purin-2-yl)piperidin-3-yl) (pyrrolidin1-yljmethanone;

or a tautomer thereof or a pharmaceutically acceptable sait of said compound or tautomer.
10 9. The compound ((R)-1 -(2-((5)-1 -(4-Chloro-1 H-pyrazol-1 -yl)ethyl)-3H-imidazo[4,5-b]pyridin-5yl)piperidin-3-yl)(pyrroiidin-1-yljmethanone, « R)-1 -(2-(( R)-1 -(4-Chloro-1 H-pyrazol-1 -yl)ethyl)-3H-imidazo[4,5-b]pyridin-5yl)piperidin-3-yl)(pyrrolidin-1-yl)methanone,

15 ((S)-1 -(2-((S)-1 -(4-Chloro-1 H-pyrazol-1 -yl)ethyl)-3H-imidazo[4,5-b]pyridin-5yl)piperidin-3-yl)(pyrrolidin-1 -yljmethanone, or ((S)-1 -(2-( (R)-1 -(4-Chloro-1 H-pyrazoi-1 -yl)ethy1)-3H-imidazo[4,5-b]pyridîn-5yl)piperidin-3-yi)(pyrrolidin-1-yl)methanone, or a mixture of thereof;

20 or a tautomer thereof or a pharmaceutically acceptable sait of said compound or tautomer.

10. The compound (R) -(1 -(2-(1 -(4-chloro-1 H-pyrazol-1 -yl)cyclopropyl)-3H-lmidazo[4,5-b]pyridin-525 yl)piperidin-3-yl)(pyrrolidin-1-yljmethanone, or (S) -( 1 -(2-( 1 -(4-ch1oro-1 H-pyrazol-1 -yl)cyclopropyl)-3H-imidazo[4,5-b]pyridîn-5yl)piperidin-3-yl)(pyrrolidin-1-yi)methanone, or a mixture of thereof;

or tautomer thereof or a pharmaceutically acceptable sait of said compound or

30 tautomer.
11 .The compound having the structure

272 or a tautomer thereof or a pharmaceutically acceptable sait of said compound or tautomer.

or a tautomer thereof or a pharmaceutically acceptable sait of said compound or tautomer.

10 13. A pharmaceutical composition comprising a compound according to any of daims 1-12 or a tautomer thereof or a pharmaceutically acceptable sait of said compound or tautomer, présent in a therapeutically effective amount, in admixture with at least one pharmaceutically acceptable excipient.
15 14. The composition of Claim 13 further comprising at least one additional pharmaceutical agent seleded from the group consisting of an anti-obesity agent, an anti-diabetic agent, and a cholesterol/lipid modulating agent.

15. The composition of Claim 14 wherein said anti-obesity agent is selected from the

20 group consisting of gut-selective MTP Inhibitors (e.g., dirlotapide, mitratapide and implitapide, R56918, CCKa agonîsts, 5HT2c agonists, MCR4 agonist, lipase inhibitor, PYYe-ae. opioid antagoniste, the combination of naltrexone with buproprion, oleoylestrone, obinepitide, pramlintide, tesofensine, leptin, liraglutide, bromocriptine, orlistat, exenatide, AOD-9604 and sibutramine.
16. The composition of Claim 14 wherein said anti-diabetic agent is selected from the group consisting of an acetyl-CoA carboxylase- (ACC) Inhibitor, a diacylglycérol O273 φ acyltransferase 1 (DGAT-1 ) inhibitor,, AZD7687, LCQ908, monoacylglycerol Oacyltransferase inhibitors, a phosphodiesterase (PDE)-10 inhibitor, an AMPK activator, a sulfonylurea, a meglitinide, an α-amylase Inhibitor, an α-glucoside hydrolase inhibitor, an α-glucosidase inhibitor, a PPARy agonist, a PPAR α/γ agonist (, a biguanide, a

5 glucagon-like peptide 1 (GLP-1) modulator such as an agonist, liraglutide, albiglutide, exenatide, albiglutide, lixisenatide, dulaglutide, semaglutide, NN-9924, TTP-054, a protein tyrosine phosphatase-1B (PTP-1B) inhibitor, SIRT-1 activator, a dipeptidyl peptîdease IV (DPP-IV) Inhibitor, an insulin secreatagogue, a fatty acid oxidatîon inhibitor, an A2 antagonist, a c-jun amino-terminal kinase (JNK) inhibitor, glucokinase

10 activators (GKa), insulin, an insulin mimetic, a glycogen phosphorylase Inhibitor, a VPAC2 receptor agonist, SGLT2 inhibitors,, aglucagon receptor modulator, GPR119 modulators, FGF21 dérivatives or analogs, TGR5 (also termed GPBAR1) receptor modulators, GPR40 agonlsts, GPR120 modulators, high affinity nicotinic acid receptor (HM74A) activators, SGLT1 inhibitors, Inhibitors or modulators of camitine paimitoyl is transferase enzymes, inhibitors of fructose 1,6-diphosphatase, inhibitors of aldose reductase, mineralocorticoid receptor inhibitors, inhibitors of TORC2, Inhibitors of CCR2 and/or CCR5, inhibitors of PKC isoforms (e.g. PKCa, ΡΚΟβ, PKCy), Inhibitors of fatty acid synthetase, Inhibitors of serine paimitoyl transferase, modulators of GPR81, GPR39, GPR43, GPR41, GPR105, Kv1,3, retinol binding protein 4, glucocorticoid

20 receptor, somatostain receptors (e.g. SSTR1, SSTR2, SSTR3 and SSTR5), inhibitors or modulators of PDHK2 or PDHK4, Inhibitors of MAP4K4, modulators of IL1 family including IL1 beta, and modulators of RXRalpha.
17. The composition of Claim 14 wherein said cholesterol/lipid moduiating agent is selected from the group consisting of HMG-CoA reductase Inhibitors; squalene synthetase inhibitors; fibrates; bile acid

25 séquestrants; ACAT inhibitors; MTP inhibitors; lipooxygenase inhibitors; choesterol absorption inhibitors; PCSK9 modulators and cholesteryl ester transfer protein inhibitors..
18. Use of a compound according to any of claims 1 · 12 or a tautomer thereof or a pharmaceutically acceptable sait of said compound or tautomer ln the manufacture of a

30 phamaceutîcal compostion for the treatment of diabètes.
19. Use of a compound of any one of claims 1 -12 or a tautomer thereof or a pharmaceutically acceptable sait of said compound or tautomer in the manufacture of a pharmaceutical composition for treating a metabolic or metabolic-related disease, condition or disorder.

274
20. Us© of compound according to any of claims 1 -12 or a tautomer thereof or a pharmaceutically acceptable sait of said compound or tautomer in the manufacture of a pharmaceutical composition for treating a condition selected from the group consisting

5 of hyperlipidemia, Type I diabètes, Type II diabètes mellitus, idiopathic Type I diabètes (Type Ib), latent autoimmune diabètes in adults (LADA), early-onset Type 2 diabètes (EOD), youth-onset atypical diabètes (YOAD), maturity onset diabètes of the young (MODY), malnutrition-related diabètes, gestational diabètes, coronaiy heart disease, ischémie stroke, restenosis after angioplasty, peripheral vascular disease, intermittent

10 claudication, myocardial infarction (e.g. necrosis and apoptosis), dyslipidemia, postprandial lipemïa, conditions of impaired glucose tolérance (IGT), conditions of impaired fasting plasma glucose, metabolic acidosis, ketosis, arthritis, obesity, osteoporosis, hypertension, congestive heart failure, left ventricular hypertrophy, peripheral arterial disease, diabetic retinopathy, macular degeneratîon, cataract, diabetic nephropathy,

15 glomerulosclerosis, chronic rénal failure, diabetic neuropathy, metabolic syndrome, syndrome X, premenstrual syndrome, coronary heart disease, angina pectoris, thrombosis, atherosclerosis, myocardial infarction, transient ischémie attacks, stroke, vascular restenosis, hyperglycemia, hyperinsulinemia, hyperlipidemia, hypertrygliceridemia, insulin résistance, impaired glucose metabolism, conditions of

20 impaired glucose tolérance, conditions of impaired fasting plasma glucose, obesity, erectile dysfunction, skin and connective tissue disorders, foot ulcérations and ulcerative colitis, endothélial dysfunction and impaired vascular compliance, hyper apo B lipoproteinemia, Alzheimer’s, schizophrénie, impaired cognition, inflammatory bowel disease, ulcerative colitis, Crohn’s disease, and irritable bowel syndrome, non-alcoholic

25 steatohepatitis (NASH), non-alcoholic fatty liver disease (NAFLD).
21. Use of a compound according to any one of claims 1 -12 or a tautomer thereof or a pharmaceutically acceptable sait of said compound or tautomer in the manufacture of (i) a first composition according to claim 13; and

275 (ii) a second composition comprising at least one additional pharmaceutical agent selected from the group consisting of an anti-obesity agent and an anti-diabetic agent, and at least one pharmaceutically acceptable excipient said composition being for treating a metabolic-related disease, condition or disorder by separate administration of said compositions to a patient in need of such treatment.
22. The use of claim 21 wherein said first composition and said second composition are for administration simultaneously.
23. The use of claim 21 wherein said first composition and said second composition are for adminstratîon sequentially and in any order.
24. A use of a DGAT2 inhibiting compound or pharmaceutically acceptable sait

15 thereof, wherein the DGAT2 inhibiting compound is a substituted 5-(piperidin-1 -yl)-3Himidazo[4,5-b]pyridine, a substituted 5-morpholino-3H-imidazo[4,5-b]pyridine, a substituted 6-(piperidin-1-yl)-1H-imidazo[4,5-b]pyrazine, a substituted 6-morpholino-1Himidazo[4,5-b]pyrazine, a substituted 2-(piperidin-1-yl)-9H-purine, or a substituted 2morpholino-9H-purine compound in the manufacture of a pharmaceutical compostion

20 for treating a disease, condition or disorder modulated by the inhibition of DGAT2 in animais.