OA17116A - Aminocyclopentanecarboxamides as chemokine receptor modulators - Google Patents

Abstract

There is provided a compound of formula I(a) or I(b)

Description

3-AMINOCYCLOPENTANECARBOXAMIDES

This application daims benefit of U.S. provisional Application No. 61/118,053 filed on November 26, 2008.

FIELD OF THE INVENTION

The présent invention relates to compounds that modulate the activity of chemokine receptors, such as CCR2 and CCR5. The compounds can be used, for example, to treat diseases associated with chemokine receptor expression or activity.

BACKGROUND OF THE INVENTION

The migration and transport of leukocytes from blood vessels into diseased tissues is involved in the initiation of normal dîsease-fighting inflammatory responses. The process, also known as leukocyte recruitment, is also related to the onset and progression of inflammatory and autoimmune diseases. The resulting pathology of these diseases dérivés from the attack of the body's immune System defenses on normal tissues. Accordingly, preventing and blocking leukocyte recruitment to target tissues in inflammatory, autoimmune disease and cancer would be an effective approach to therapeutic intervention.

The infiltration of monocytes/macrophages into sites of inflammation is related to proteins, such as monocyte chemoattractant protein-1 (MCP-1, CCL2). Macrophages produce chemokines, such as macrophage inflammatory protein-1 beta (MlP-1 B, CCL4). Such proteins interacts with chemokine receptors, for example, CCR2 and CCR5. Modulation, such as antagonism or inhibition, of CCR2 or CCR5 would be helpful to treat a wide range of diseases.

The identification of compounds that modulate the activity of chemokine receptors represents a désirable drug design approach to develop pharmacological agents for the treatment of diseases associated with chemokine receptor activity. The compounds of the présent invention help fulfill these and other needs.

SUMMARY OF THE INVENTION

In an embodiment of the présent invention, there is provided a compound of Formula l(a) or l(b):

l(b) or a pharmaceutically acceptable sait thereof, wherein:

A is O or CF2;

W is CR¹³R¹⁴, C(O), CHOR¹⁵, CHF, CF₂, O or S

R¹ is H or C₁₆ alkyl optionally substituted by 1-3 substituents selected from halo, OH, CO₂H, CO₂-(Ci-6 alkyl), or Ci-₃alkoxy;

R² and R³ are each, independently, H, (Ci-Ce)alkyl, (CrCejhaloalkyl, halo, (C3-C6)cycloalkyl, CN, OH, CO2R, OCOR¹²; wherein said (Ci-C6)alkyl is optionally substituted with one or more substituents selected from F, (C1-C3) alkoxy, OH, CN or CO₂R¹²;

R⁸ and R⁹ are each, independently, H, (Ci-C6)alkyl, (Ci-C6)haloalkyl, (C3C6)cycloalkyl, (C3-C6)cycloalkyloxy, CN, OH, CO2R, OCOR¹²; wherein said (Cr

C₆)alkyl is optionally substituted with one or more substituents selected from F, (C_rC₃)alkoxy, OH, CN or CO₂R¹²;

R² and R⁹, taken together may form a 5-8 membered ring;

R⁴ and R⁷, taken together may form a 5-8 membered ring.

R⁴ and R⁵ are each, independently, H, CN, (CrC6)alkyl, halo, (CrC3) alkoxy, (Ci-C3)haloalkoxy, (C3-Ce)cycloalkyl, OH, CO2R¹², OCOR¹², wherein said (CrC6)alkyl is optionally substituted with one or more substituents selected from F, (Ci-C₃) alkoxy, OH or CO₂R¹²;

R⁶ and R⁷ are each, independently, H, CN, (Ci-Ce)alkyl, halo, (CiC3)alkoxy, (Ci-C3)haloalkoxy, (C3-C6)cycloalkyl, OH, CO2R¹², OCOR¹² , wherein said (Ci-Cejalkyl is optionally substituted with one or more substituents selected from F, (Ci-C3)alkoxy, OH or CO₂R¹²;

or R² and R³ together with the carbon atom to which they are attached form a 3-7 membered spirocyclyl group;

or R⁴ and R⁵ together with the carbon atom to which they are attached form a 4-7 membered spirocyclyl group;

or R³ and R⁴ together with the C atoms to which they are attached form a fused 3-7 membered cycloalkyl group or 3-7 membered heterocycloalkyl group;

R¹⁰ is (CrC₆)alkyl, (CrCeJhaloalkyl, (Ci-C₆)hydroxyalkyl, (Ci-C₆)alkoxy, (C₃-C₆)cycloalkyl_t hydroxy(C₃-C₆)cycloalkyl, (Ci-C₆)alkoxy cycloalkyl, OH, (CiC₅)heterocyclyl, amino, aryl or CN,

R¹¹ is aryl or heteroaryl, said R¹¹ optionally, independently substituted by one or more (Ci-C₆) alkyl, halo, (Ci-C₆)haloalky (CrC₆)alkoxy, OH, amino, C(O)NH₂, NH₂SO₂, SF₅,orCN;

R¹²is H, (CrC4)alkyl, or (C₃-C₆)cycloalkyl;

R¹³ is H, halo, (Ci-C₄)alkyl, (Ci-C₄)alkoxy,or OH;

R¹⁴ is H, halo, (Ci-C₄)alkyl, (Ci-C₄)alkoxy or OH; and

R¹⁵ is H .(CrCeJalkyl or (C₃-C₆)cycloalkyl.

DETAILED DESCRIPTION OF THE INVENTION

These inventions are not limited to the embodiments described in this spécification, and may be modified.

A. Définitions

For the following defined terms, these définitions shall be applied, unless a different définition is given in the daims or elsewhere in this spécification.

The term aryl groups is intended to include aromatic carbocylic groups such as phenyl, biphenylyl, indenyl, naphthyl as well as aromatic carbocycles fued to a heterocycle such as benzothienyl, benzofuranyl, indolyl, quinolinyl, benzothiazole, benzooxazole, benzimidazole, isoquinolinyl, isoindolyl, benzotriazole, indazole, and acridinyl.

The term heteroaryl includes mono- and poly-cyclic aromatic rings containing from 3 to 20, or from 4 to 10 ring atoms, at least one of which is a heteroatom such as oxygen, sulphur, phosphorus or nitrogen. Examples of such groups include furyl, thienyl, pyrrolyl, imidazolyl, triazolyl, thiazolyl, tetrazolyl, oxazolyl, isoxazolyl, pyrazolyl, pyridyl, pyrimidinyl, pyrazinyl, pyridazinyl, triazinyl, quinolinyl, iosquinolinyl, quinoxalinyl, benzthiazolyl, benzoxazolyl, benzothienyl or benzofuryi.

The term “cycloalkyl, refer to cyclized hydrocarbons (mono and polycyclic) such as cyclized alkyl, alkenyl, or alkynyl groups. In some embodlments, the cycloalkyl group js C₃.i₄₁ C₃.i₀, C₃._S1 C₃.₇, C₃.₆, or C₃.₅. In some embodïments, cycloalkyl moieties each hâve from 3 to 14, from 3 to 10, or from 3 to 6 ring-forming carbon atoms. In some embodlments, the cycloalkyl group has 0, 1 or 2 double or triple bonds. Examples of cycloalkyl groups înclude cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, cycloheptyl, cyclopentenyl, etc. In the présent application, cycloalkyl is also intended to înclude bridged cyclic hydrocarbons such as adamantyl groups and the like.

Heterocycles are carbocyclic rings (mono or polycyclic) which include one or more heteroatoms such as nitrogen, oxygen or sulfur in the ring. In some embodïments, the heterocycle contains 3 to 8 ring members. In some embodïments, the heterocycle contains 3 to 6 ring members. In some embodïments, the heterocycle contains 1, 2 or 3 heteroatoms. Heterocycles can be saturated or unsaturated. In some embodïments, heterocycles contain 0,1 or 2 double bonds or triple bonds. Ring-forming carbon atoms and heteroatoms can also bear oxo or sulfide substituents (such as, CO, CS, SO, SO₂, NO, and the like.). Examples of heterocycles include tetrahydrofuranyl, tetrahydrothiophenyl, morpholino, thiomorpholino, azetidinyl, pyrrolidinyl, piperazinyl, piperidinyl, pyrane, dioxane, and thiazolidinyl.

Monosubstituted aryl refers to an aryl group having one substituent. Polysubstituted aryl refers to aryl having 2 or more substitutents (such as 2-4 substituents). Monosubstituted heteroaryl refers to a heteroaryl group having one substituent. Polysubstituted heteroaryl refers to heteroaryl having 2 or more substitutents (such as 2-4 substituents). Monosubstituted cycloalkyl (or carbocycle) refers to a cycloalkyl group having one substituent. Polysubstituted cycloalkyl (or carbocycle) refers to cycloalkyl having 2 or more substitutents (such as 2-4 substituents). Monosubstituted heterocycle refers to a heterocycle having one substituent. Polysubstituted heterocycle refers to heterocycle having 2 or more substitutents (such as 2-4 substituents).

The term halo, by itself or as part of another substituent, means, unless otherwise stated, a fluorine, chlorine, bromine, or iodine. Similarly, terms such as haloalkyl, are meant to include monohaloalkyl and polyhaloalkyl. For example, the term haloalkyl, such as halo(Ci-C4)alkyl, is meant to include trifluoromethyl, 2,2,2-trifluoroethyl, 4-chlorobutyl, 3-bromopropyl, and the like.

The term alkyl when used either alone or as a suffix includes straight chain and branched structures such as primary alkyl groups, secondary alkyl groups and tertiary alkyl groups. These groups may contain up to 15, or up to 8, or up to 4 carbon atoms. In some embodiments, the alkyl group is Ci._10l C^e, C^e, Ci-₅, C-i-4, or C1.3. Examples of alkyl radicals include groups such as methyl, ethyl, npropyl, îsopropyl, n-butyl, t-butyl, isobutyl, and sec-butyl. Similarly the terms alkenyl and alkynyl refer to unsaturated straight or branched structures containing for example from 2 to 12, or from 2 to 6 carbon atoms. In some embodiments, the alkenyl or alkynyl group is C₂-io> C2-8. C2-6. C₂.₅, C₂.₄, or C2-3. Examples of alkenyl and alkynyl groups include vinyl, 2-propenyl, crotyl, 2isopentenyl, 2-(butadienyl), 2,4-pentadienyl, 3-(1,4-pentadienyl), ethynyl, 1- and 3-propynyl, 3-butynyl, and the higher homologs and isomers.

The term carrier” describes an ingrédient other than a compound. Carriers may be pharmaceutically acceptable matériel or vehicle. Examples include liquid or solid Aller, diluent, excipient, solvent or encapsulating material.

The phrase “contacting a chemokine receptor means in vivo, ex vivo, or in vitro contact is made with a chemokine receptor and includes administration of a compound or sait of the présent invention to a subject having a chemokine receptor, as well as, for example, introducing a compound or sait of the invention into a sample containing a cellular, unpurified, or purified préparation containing a chemokine receptor. For example, contacting includes interactions between the compound and the receptor, such as binding.

The term subject” refers to any animal, including mammals, such as mice, rats, other rodents, rabbits, dogs, cats, pigs, cattle, sheep, horses, primates, or humans.

The term “treating” (and corresponding terms “treat” and treatment”) includes palliative, restorative, or preventative (prophylactic) treating of a subject. The term “palliative treating” refers to treatment that eases or reduces the effect or intensity of a condition in a subject without curing the condition. The term “preventative treating” (and the corresponding term prophylactic treating) refers to treatment that prevents the occurrence of a condition in a subject. The term “restorative treating (curative) refers to treatment that halts the progression of, reduces the pathologie manifestations of, or entirely éliminâtes a condition in a subject. Treating can be done with a therapeutically effective amount of compound, sait or composition that elicits the biological or médicinal response of a tissue, System or subject that is being sought by an individual such as a researcher, doctor, veterinarian, orclinician.

B. Compounds

In an embodiment of the présent invention, there is provided a compound of l(a) or l(b):

or a pharmaceutically acceptable sait thereof, wherein: A is O or CF2;

W is CR¹³R¹⁴, C(O), CHOR¹⁵, CHF, CF₂, O or S

R¹ is H or Ci-e alkyl optionally substituted by 1-3 substituents selected from halo, OH, CO₂H, 00₂-(0₁₄alkyl), or C-|.₃ alkoxy;

R² and R³ are each, independently, H, (Ci-C6)alkyl, (CrC6)haloalkyl, halo, (C3-C6)cycloalkyl, CN, OH, CO2R, OCOR¹²; wherein said (Ci-Ce)alkyl is optionally substituted with one or more substituents selected from F, (C-iC3)alkoxy, OH, CN or CO₂R¹²;

R⁸ and R⁹ are each, independently, H, (CrC6)alkyl, (CrC6)haloalkylt (C3Cejcycloalkyl, CN, OH, CO2R, OCOR¹²; wherein said (Ci-C6)alkyl is optionally substituted wîth one or more substituents selected from F, (CrC₃)alkoxy, OH, CN or CO₂R¹²;

R² and R⁹, taken together may form a 5-8 membered ring;

R⁴ and R⁷, taken together may form a 5-8 membered ring.

R⁴ and R⁵ are each, independently, H, CN, (C-t-CeJalkyl, halo, (Cr C3)alkoxy, (Ci-C3)haloalkoxy, (C3-C6) cycloalkyl, OH, CO2R¹², OCOR¹², wherein said (CrC6)alkyl is optionally substituted with one or more substituents selected from F, C1-3 alkoxy, OH or CO₂R¹²;

R⁶ and R⁷ are each, independently, H, CN, (Ci-Cejalkyl, halo, (CiC3)alkoxy, (Ci-C3)haloalkoxy, (C3-C6)cycloalkyl, OH, CO2R¹², OCOR¹² , wherein said (CrCe)alkyl is optionally substituted with one or more substituents selected from F, (C1-C3) alkoxy, OH or CO2R¹²;

or R² and R³ together with the carbon atom to which they are attached form a 3-7 membered spirocyclyl group;

or R⁴ and R⁵ together with the carbon atom to which they are attached form a 3-7 membered spirocyclyl group;

or R³ and R⁴ together with the C atoms to which they are attached form a fused 3-7 membered cycloalkyl group or 3-7 membered heterocycloalkyl group;

R¹⁰ is (CrCe)alkyl, (CrCeJhaloalkyl, (Ci-C₆)hydroxyalkyl, (Ci-C₆)alkoxy, (C₃-C₆)cycloalkyl, hydroxy(C₃-C6)cycloalkyl, alkoxy cycloalkyl, OH, (C_r C₅)heterocyclyl, amino, aryl or CN,

R¹¹ is aryl or heteroaryl, said R¹¹ optionally, independently substituted by one or more (C_rC₆) alkyl, halo, (C-iCgJhaloalky (Ci-Ce)alkoxy, OH, amino, C(O)NH₂, NH₂SO₂₎ SF₅,orCN;

R¹²is H, (Ci-C4)alkyl, or (C₃-C₆)cycloalkyl;

R¹³ is H, halo, (CpC^alkyl, (CrC^alkoxy.or OH;

R¹⁴ is H, halo, (Ci-C₄)alkyl, (Ci-C₄)alkoxy or OH; and

R¹⁵ is H, (C-i-CeJalkyl or (C₃-C₆)cycloalkyl.

In another embodiment of the présent invention, there is provided a compound of Formula II:

or a pharmaceutically acceptable sait thereof, wherein:

R¹ is H, (Ci-Cejalkyl or cyclopropyl, said (Ci-Ce)alkyl optionally substituted by halo, CN, C(O)OH or OH;

R² is (C₁-C₆)alkyl (Ci.C₆)haloalkyl or (Cs-Cejcycloalkyl;

R³ R⁴, R⁵, R⁶, R⁷, R⁸ and R⁹ are independently H, (C1-C4) alkyl, CN_t halo or amino;

R¹⁰ is (Ci-C₆)alkyl, (CrC₆)haloalkyl, (Ci-C₆)hydroxyalkyl, (Ci-C₆)alkoxy, (C₃Cejcycloalkyl, hydroxy(C₃-C₆)cycloalkyl, alkoxy (C₃-C6)cycloalkyl, OH, (C_r Csjheterocyclyl, amino, aryl or CN;

R¹¹ is aryl or heteroaryl, said R¹¹ optionally, independently substituted by one or more (Ci-C₆) alkyl, halo, (CrCeJhaloalky (CrC₆)alkoxy, OH, amino, C(O)NH₂, NH2SO2, SF₅ or CN;

W is CR¹³R¹⁴, C(O), CHOR¹⁵, CHF, CF_2i O or S;

R¹³ and R¹⁴ are independently H, halo, (Ci-C4)alkyl, (Ci-C4)alkoxy,or OH; and

R¹⁵ is H, (CrCeJalkyl or (C3-C₆)cycloalkyl.

H

In another embodiment, there is provided a compound of Formula II, or a pharmaceutically acceptable sait thereof, wherein:

R¹ is H or (Ci-C₆)alkyl

R² is (Ci-C₆)alkyl or (CrC₆)haloalkyl

R³, R⁴, R⁵, R⁶, R⁷, R⁸ and R⁹ are each H;

R¹¹ is

wherein X, Y, Z, Q and D are independently N or CR¹⁶, and wherein 0, 1,2 or 3 of X, Y, Z, Q and D are N; and wherein T, U and V are independently selected from CH, N, S, or O, provided that T and U are not both simultaneously O or S;

each R¹⁶ is independently H, halo, (Ci-C₆)alkyl, (Ci-C₆)haloalkyl, (Ci-C₆)alkoxy or CN;

R¹⁷ is H, (Ci-C₆)alky, (Ci-C₆)haloalky, or (Ci-C₆)alkoxy; and

W is CR¹³R¹⁴, C(O), CHOR¹⁵, CHF or CF₂.

In some embodiments of the compound of Formula II, or a pharmaceutically acceptable sait thereof, R² is methyl.

In some embodiments of the compound of Formula II, or a pharmaceutically acceptable sait thereof,

R¹ is H, (C-t-C₆)alkyl or cyclopropyl, said (C-i-C^alkyl optionally substituted by halo, CN, C(O)OH or OH;

R² is methyl;

R³ R⁴, R⁵, R⁶, R⁷, R⁸ and R⁹ are independently H, C_rC4 alkyl, CN, halo or amino;

R¹⁰ is (Ci-C₆)alkyl, (CrCe)haloalkyl, (C_rC₆)hydroxyalkyl, (Ci-C₆)alkoxy, (C₃C₆)cycloalkyl, hydroxy(C3-C₆)cycloalkyl, alkoxy (C3-C₆)cycloalkyl, OH, (Cr C₅)heterocyclyl, amino, aryl or CN;

R¹¹ is aryl or heteroaryl, said R¹¹ optionally, independently substituted by one or more (C_rC₆) alkyl, halo, (Ci-Cejhaloalky (CrCeJalkoxy, OH, amino, C(O)NH₂, NH₂SO₂, SF₅ orCN;

W is CR¹³R¹⁴, C(O), CHOR¹⁵, CHF, CF_2i O or S;

R¹³ and R¹⁴ are independently H halo, (CrC₄)alkyl, (CrC4)alkoxy,or OH; and

R¹⁵ is H, (Ci-C₆)aikyl or (C₃-C6)cycloalkyl.

In some embodiments of the compound of Formula II, or a pharmaceutically acceptable sait thereof, R¹⁰ is

H or (Ci-C₆)alkyl

In some embodiments of the compound of Formula II, or a pharmaceutically acceptable sait thereof,

R¹ is H, (Ci-C₆)alkyl or cyclopropyl, said (Ci-Cô)alkyl optionally substituted by halo, CN, C(O)OH or OH;

R² is methyl;

R³ R⁴, R⁵, R⁶, R⁷, R⁸ and R⁹ are independently H, C1-C4 alkyl, CN, halo or amino;

R¹⁰is

R¹¹ is aryl or heteroaryl, said R¹¹ optionally, independently substituted by one or more (Ci-C₆) alkyl, halo, (CiC₆)haloalky (Ci-C₆)alkoxy, OH, amino, C(O)NH2, NH₂SO₂, SF₅ orCN;

W is CR¹³R¹⁴, C(0), CHOR¹⁵, CHF, CF_2i O or S;

R¹³ and R¹⁴ are independently H halo, (C-i-C4)alkyl, (Ci-C4)alkoxy,or OH; 5

R¹⁵ is H, (Ci-Ce)alkyl or (C3-C₆)cycloalkyl; and

R¹⁰ is H or (Ci-C_e)alkyl.

In some embodiments of the présent invention, there is provided a compound of Formula II, or a pharmaceutically acceptable sait thereof, wherein R¹¹ is

In some embodïments of the présent invention, there is provided a compound of Formula II, or a pharmaceutically acceptable sait thereof, wherein R¹ is H, (Ci-C₆)alkyl or cyclopropyl, said (Ci-C₆)alkyl optionally substituted by halo, CN, C(O)OH or OH;

R² is methyl;

R³ R⁴, R⁵, R⁶, R⁷, R⁸ and R⁹ are independently H, C_rC₄ alkyl, CN, halo or amino;

R¹⁰ is

W is CR¹³R¹⁴, C(0), CHOR¹⁵, CHF, CF₂, O or S;

R¹³ and R¹⁴ are independently H halo, (Ci-C₄)alkyl, (C_rC₄)alkoxy_tor OH; and

R¹⁵ is H, (Ci-C₆)alkyl or (C₃-C₆)cycloalkyl.

In some embodiments, there is provided a compound of Formula II, or pharmaceutically acceptable sait thereof, wherein R¹⁰ is

ch₃ h₃c , or ^h3^c and

In some embodiments, there is provided a compound of Formula II, or pharmaceutically acceptable sait thereof, wherein

R¹ is H;

R² is methyl;

R³ R⁴, R⁵, R⁶, R⁷, R⁸ and R⁹ are each H;

W is CH₂.

In another embodiment, there is provideded compound, or pharmaceutically acceptable sait thereof, selected from the group consisting of: 1,5-anhydro-2,3-dideoxy-3-{[(1R,3S)-3-ethyl-3-({(1S,4S)-5-[4(trifluoromethyl)pyridin-2-yl]-2,5-diazabicyclo[2.2.1]hept-2yl}carbonyl)cyclopentyl]amino}-4-O-methyl-D-erythro-pentitol;

1,5-anhydro-2,3-dideoxy-3-{[(1R,3S)-3-ethyl-3-({(1S,4S)-5-[4(tnfluoromethyl)pynmidin-2-yl]-2,5-diazabicyclo[2.2.1]hept-2yl}carbonyl)cyclopentyl]amino}-4-O-methyl-D-erythro-pentitol;

1,5-anhydro-2,3-dideoxy-3-{[(1R,3S)-3-ethyl-3-({(1S,4S)-5-[6(trifluoromethyl)pyrimidin-4-yl]-2,5-diazabicyclo[2.2.1]hept-2yl}carbonyl)cyclopentyl]amino}-4-O-methyl-D-erythro-pentitol;

1,5-anhydro-2,3-dideoxy-3-{[(1R,3S)-3-ethyl-3-({(1S,4S)-5-[6(trifluoromethyl)pyrazin-2-yl]-2,5-diazabicyclo[2.2.1]hept-2’ yl}carbonyl)cyclopentyl]amino}-4-O-methyl-D“erythro-pentitol;

1,5-anhydro-2,3-dideoxy-3-{[(1 R,3S)-3-ethyl-3-({(1S,4S)-5-[2(trifluoromethyl)pyrimidin-4-yl]-2,5-diazabicyclo[2.2.1]hept-2yl}carbonyl)cyclopentyl]amino}-4-O-methyl-D-erythro-pentitol;

1,5-anhydro-2,3-dideoxy-3-{[(1R,3S)-3-ethyl-3-({(1S,4S)-5-[6(trifluoromethyl)pyndin-2-yl]-2,5-diazabicycîo[2.2.1]hept-2yl}carbonyl )cyclopentyl]amino}-4-O-methy[-D-erythrO“pentitol ;

1,5-anhydro-2,3-dideoxy-3-{[(1R,3S)-3-ethyl-3-({(1S,4S)-5-[2(trifluoromethyl)pyndin-4-yl]-2,5-diazabicyclo[2.2.1]hept-2yl}carbonyl)cyclopentyl]amino}-4-O-methyl-D-erythro-pentitol;

1,5-anhydro-2,3-dideoxy-3-{[(1 R, 3S)-3-ethyl-3-(((13,43 )-5-[3-fluoro-4(trifluoromethyl)pyridin-2-yl]-2,5-diazabicyclo[2.2.1]hept-2yl}carbonyl)cyclopentyl]amino}-4-O-methyl-D-erythro-pentitol;

1,5-anhydro-2,3-dideoxy-3-{[(1R,3S)-3-methyl-3-({(1S,4S)-5-[4(tnfluoromethyl)pyridin-2-yl]-2,5-diazabicyclo[2.2.1]hept-2yl}carbonyl)cyclopentyl]amino}-4-O-methyl-D-erythro-pentitol;

I.S-anhydro^.S-dideoxy-S-ÎKIR.SSJ-S-methyl-S-iitlS^SJ-S-fe(tnfluoromethyl)pyrazin-2-yl]-2,5-diazabicyclo[2.2.1]hept-2yl}carbonyl)cyclopentyl]amino)-4-O-methyl-D-erythro-pentitol;

1,5-anhydro-2,3-dideoxy-3-{[(1R,3S)-3-isopropyl-3-({(1S,4S)-5-[4(trÎfluoromethyl)pyndin-2-yl]-2,5-diazabicyclo[2.2.1]hept-2yl}carbonyl)cyclopentyl]amino}-4-O-niethyl-D-erythro-pentitol;

1,5-anhydro-2,3-dideoxy-3-{[(1 R,3S)-3-isopropyl-3-({( 1 S,4S)-5-[4(trifluoromethyl)pyrimidin-2-yl]-2,5-diazabicyclo[2.2.1]hept-2yl}carbonyl)cyclopentyl]amino}-4-O-methyl-D-erythro-pentitol;

1,5-anhydro-2,3-dideoxy-3-{((1R,3S)-3-isopropyl-3-({(1S,4S)-5-[6(trifluoromethyl)pyrimidin-4-yl]-2,5-diazabicyclo[2.2.1]hept-2yl}carbonyl)cyclopentyl]amino}-4-O-methyl-D-erythro-pentitol;

1,5-anhydro-2,3-dideoxy-3-{[(1R,3S)-3-isopropyl-3-({(1S,4S)-5-[6(trifluoromethyl)pyrazin-2-yl]-2,5-diazabicyclo[2.2.1]hept-2yl}carbonyl)cyclopentyl]amino}-4-O-methyl-D-erythro-pentitol;

1,5-anhydro-2,3-dideoxy-3-{[(1R,3S)-3-isopropyl-3-({(1S,4S)-5-[2(trifluoromethyl)pyrimidin-4-yl]-2,5-diazabicyclo[2.2.1]hept-2yl}carbonyl)cyclopentyl]amino}-4-O-methyl-D-erythro-pentitol;

1,5-anhydro-2,3-dideoxy-3-{[(1 R,3S)-3-isopropyl-3-({(1S,4S)-5-[6(trifluoromethyl)pyridin-2-yl]-2,5-diazabicyclo[2.2.1]hept-2yl}carbonyl)cyclopentyl]amino}-4-O-methyl-D-erythro-pentitol;

1,5-anhydro-2,3-dideoxy-3-{[(1 R,3S)-3-isopropyl-3-({(1S,4S )-5-(2(trifluoromethyl)pyridin-4-yl]-2,5-diazabicyclo[2.2.1]hept-2yl}carbonyl)cyclopentyl]amino}-4-O-methyl-D-erythro-pentitol;

1,5-anhydro-2_t3-dideoxy-3-{[( 1 R,3S)-3-isopropyl-3-({( 1S ,4S )-5-(5(trifluoromethyl)pyridazin-3-yl]-2,5-diazabicyclo[2.2.1]hept-2yl}carbonyl)cyclopentyl]amino)-4-O-methyl-D-erythro-pentitol;

1,5-anhydro-3-{[(1 R,3S)-3-isopropyl-3-({(1 S,4S )-5-(2,6-bis(trîfluoromethyl)pyridin4-yl]-2₁5-diazabicyclo[2.2.1]hept-2-yl}carbonyl)-3-isopropylcyclopentyl]amino}2,3-dideoxy-4-O-methyl-D-erythro-pentitol;

1,5-anhydro-2,3-dideoxy-3-{[(1R,3S)-3-({(1S,4S)-5-[3-fluoro-4(trifluoromethyl)pyridin-2-yl]-2,5-diazabicyclo[2.2.1]hept-2-yl}carbonyl)-3isopropylcyclopentyl]amino}-4-O-methyl-D-erythro-pentitol;

1,5-anhydro-2,3-dideoxy-3-{[(1R,3S)-3-isopropyl-3-({(1S,4S)-5-[6-methyl-4(trifluoromethyl)pyridin-2-yl]-2,5-diazabicyclo[2.2.1]hept-2yl}carbonyl)cyclopentyl]amino}-4-O-methyl-D-erythro-pentitol;

1,5-anhydro-2,3-dideoxy-3-{[(1R,3S)-3-isopropyl-3-({(1S,4S)-5-[3(trifluoromethyl)phenyl]-2,5-diazabicyclo[2.2.1]hept-2yl}carbonyl)cyclopentyl]amino}-4-O-methyl-D-erythro-pentitol;

1,5-anhydro-2,3-dideoxy-3-{[(1R,3S)-3-({(1S,4S)-5-[3-fluoro-5(trifluoromethyl)phenyl]-2,5-diazabicyclo[2.2.1]hept-2-yl}carbonyl)-3isopropylcyclopentyl]amino}-4-O-methyl-D-erythro-pentitol;

1,5-anhydro-2,3-dideoxy-3-{[(1R,3S)-3-(2,2-difluoroethyl)-3-({(1S,4S)-5-[6(trifluoromethyl)pyrimidin-4-yl]-2,5-diazabicyclo[2.2.1]hept-2yl}carbonyl)cyclopentyl]amino}-4-O-methyl-D-erythro-pentitol;

1,5-anhydro-2,3-dideoxy-3-{((1 R,3S)-3-(2_t2-difluoroethyl)-3-({(1S,4S)-5-[4(trifluoromethyl)pyridin-2-yl]-2_l5-diazabicyclo[2.2.1]hept-2yl}carbonyl)cyclopentyl]amino}-4-O-methyl-D-erythro-pentitol;

1,5-anhydro-2,3-dideoxy-3-{[(1 R,3S )-3-(2,2-difluoroethyl )-3-({(1 S,4S)-5-[2(trifluoromethyl)pyridin-4-yl]-2,5-diazabicyclo[2.2.1]hept-2yl}carbonyl)cyclopentyl]amino}-4-O-methyl-D-erythro-pentitol;

1,5-anhydro-2,3-dideoxy-3-{[(1R,3S)-3-(2,2“difluoroethyl)-3-({(1S,4S)-5-[4(trifluoromethyl)pyrimidin-2-yl]-2,5-diazabicyclo[2.2.1]hept-2yl}carbonyl)cyc!opentyl]amino}-4-O-methyl-D-erythro-pentitol 1,5-anhydro-2,3-dideoxy-3-{[(1R,3S)-3-(2,2-difluoroethyl)-3-({(1S,4S)-5-[6(trifluoromethyl)pyridin-2-yl]-2,5-diazabicyclo[2.2.1]hept-2yl}carbonyl)cyclopentyl]amino}-4-O-methyl-D-erythro-pentitol;

1,5-anhydro-2,3-dideoxy-3-{[(1 R,3S )-3-(2,2-difluoroethyl)-3-({(1S,4S)-5-[6(trifluoromethyl)pyrazin-2-yl]-2,5-diazabicyclo[2.2.1]hept-2yl}carbonyl)cyclopentyl]amino}-4-O-methyl-D-erythro-pentitol;

1,5-anhydro-2,3-dideoxy-3-{[(1R,3S)-3-(2,2-difluoroethyl)-3-({(1S,4S)-5-[2(trifluoromethyl)pyrimidin-4-yl]-2,5-diazabicyclo[2.2.1]hept-2yl}carbonyl)cyclopentyl]amino}-4-O-methyl-D-erythro-pentitol;

1,5-anhydro-2,3-dideoxy-3-{[(1 R,3S )-3-(2,2-difluoroethyl )-3-({(1 S,4S)-5-[5(trifluoromethyl)pyridazin-3-yl]-2,5-diazabicyclo[2.2.1]hept-2yl}carbonyl)cyclopentyl]amino}-4-O-methyl-D-erythro-pentitol;

1,5-anhydro-2,3-dideoxy-4-O-methyl-3-{[(1 R,3S )-3-(2,2,2-trifluoroethyl )-3({(1 S,4S)-5-[6-(trifluoromethyl)pyrimidin-4-yl]-2,5-diazabicyclo[2.2.1]hept-2yl}carbonyl)cyclopentyl]amino}-D-erythro-pentitol;

1,5-anhydro-2,3-dideoxy-4-O-methyl-3-{[(1R,3S)-3-(2,2,2-trifluoroethyl)-3({(1S,4S)-5-[4-(trifluoromethyl)pyridin-2-yl]-2,5-diazabicyclo[2.2.1]hept-2yl}carbonyl)cyclopentyl]amino}-D-erythro-pentit;

7,5-anhydro-2,3-dideoxy-4-O-methyl-3-{[(1R,3S)-3-(2,2,2-trifluoroethyl)-3({(1S,4S)-5-[2-(trifluoromethyl)pyridin-4-yl]-2,5-diazabicyclo[2.2.1]hept-2yl}carbonyl)cyclopentyl]amino}-D-erythro-pentitol;

1,5-anhydro-2,3-dideoxy-4-O-methyl-3-{[(1R₁3S)-3-(2,2,2-trifluoroethyl)-3({(1S,4S)-5-[4-(trifluoromethyl)pyrimidin-2-yl]-2,5-diazabicyclo[2.2.1]hept-2yl}carbonyl)cyclopentyl]amino)-D-erythro-pentitol;

1,5-anhydro-2,3-dideoxy-4-O-methyl-3-{[(1R,3S)-3-(2,2,2-trifluoroethyl)-3({(1S,4S)-5-[6-(trifIuoromethyl)pyridin-2-yl]-2,5-diazabicyclo[2.2.1]hept-2yl}carbonyl)cyclopentyl]amino}-D-erythro-pentitol;

1,5-anhydro-2,3-dideoxy-4-O-methyl-3-{[(1R,3S)-3-(2,2,2-tnfluoroethyl)-3({(1S,4S)-5-[6-(trifluoromethyl)pyrazin-2-yl]-2,5-diazabicyclo[2.2.1 ]hept-2yl}carbonyl)cyclopentyl]amino}-D-erythro-pentitol;

1,5-anhydrO-2,3-dideoxy-4-O-methyl-3-{[(1 R,3S )-3-(2,2,2-trifluoroethyl )-3({(1S,4S)-5-[2-(trifluoromethyl)pyrimidin-4-yl]-2_I5-diazabicyclo[2.2.1]hept-2yl}carbonyl)cyclopentyl]amino}-D-erythro-pentitol

1,5-anhydro-2_l3-dideoxy-4-O-methyl-3-{[(1R,3S)-3-(2,2,2-trifluoroethyl)-3({(1S,4S)-5-[5-(trifluoromethyl)pyridazin-3-yl]-2,5-diazabicyclo[2.2.1]hept-2yl}carbonyl)cyclopentyl]amino}-D-erythro-pentitol;

1,5-anhydro-2,3-dideoxy-3-{[(1 S,4S)-4-isopropyll-4-({( 1 S,4S)-5-[4(trifluoromethyl)pyridin-2-yl]-2,5-diazabicyclo[2.2.1]hept-2-yl}carbonyl)cyclopent2-en-1-yl]amino}-4-O-methyl-D-erythro-pentitol;

1,5-anhydro-2,3-dideoxy-3-{[(1 S,4S)-4-isopropyll-4-({( 1S ,4S )-5-[4(trifluoromethyl)pyrimÎdin-2-yl]-2,5-dÎazabicyclo[2.2.1]hept-2yl}carbonyl)cyclopent-2-en-1-yl]amino}-4-O-methyl-D-erythro-pentitol;

1,5-a n h yd ro-2,3-d id e oxy-3- {[( 1S ,4S ) -4-i sop ro py 11 -4-( {( 1S, 4S )-5-(6(trifluoromethyl)pyridin-2-yl]-2,5-diazabicyclo[2.2.1]hept-2-yl}carbonyl)cyclopent2-en-1-yl]amino}-4-O-methyl-D-erythro-pentitol;

1,5-anhydro-2,3-dideoxy-3-{[(1S,4S)-4-isopropyll-4-({(1S,4S)-5-[6(trifluoromethyl)pyrazin-2-yl]-2,5-diazabicyclo[2.2.1]hept-2-yl}carbonyl)cyclopent2-en-1-yl]amino}-4-O-methyl-D-erythro-pentitol;

1,5-anhydro-3-{[( 1 R,3S)-3-{[(1S,4S)-5-(tert-butoxycarbonyl)-2,5diazabicyclo[2.2.1]hept-2-yl]carbonyl}-3-ethylcyclopentyl]amino}-2,3-dideoxy-4-Omethyl-D-erythro-pentitol ;

1,5-anhydro-3-{[(1R,3S)-3-{[(1S,4S)-5-(tert-butoxycarbonyl)-2,5diazabicyclo[2.2.1]hept-2-yl]carbonyl}-3-(2,2,2-trifluoroethyl)cyclopentyl]amino}2,3-dideoxy-4-O-methyl-D-erythro-pentitol;

1,5-Anhydro-2,3-dideoxy-3-{[(1R,3S)-3-(1-hydroxycyclobutyl)-3-({(1S,4S)-5-[6(trifluoromethyl)pyridin-2-yl]-2,5-diazabicyclo[2.2,1]hept-2yl}carbonyl)cyclopentyl]amino}-4-O-methyl-D-erythro-pentitol;

1,5-Anhydro-2,3-dideoxy-3-{[(1 R,3S)-3-(1-hydroxycyclobutyl)-3-({(1S,4S )-5-(4(trifluoromethyl )pyridin-2-yl]-2,5-diazabicyclo[2.2.1 ]hept-2yl}carbonyl)cyclopentyl]amino}-4-O-methyl-D-erythro-pentitol;

1,5-Anhydro2,3-dideoxy-3-{[(1 R,3S)-3-(1-hydroxycyclobutyl)-3-({(1S,4S)-5-[4(trifluoromethyl )pyrimidin-2-yl]-2,5-diazabicyclo[2.2.1]hept-2yl}carbonyl)cyclopentyl]amino}-4-O-methyl-D-erythro-pentitol;

1,5-Anhydro-2,3-dideoxy-3-{[(1 R,3S )-3-( 1-hydroxycyclobutyl)-3-({(1S,4S )-5-(2(tnfluoromethyl)pyrimidin-4-yl]-2,5-diazabicyclo[2.2.1]hept-2yl}carbonyl)cyclopentyl]amino}-4-O-methyl-D-erythro-pentitol _t5-anhydro-2,3-dideoxy-3-{[(1 R,3S )-3-( 1-hydroxycyclobutyl )-3-( {(1 S,4S)-5-[6(tnfluoromethyl)pyrimidin-4-yl]-2,5-diazabicyclo[2.2.1]hept-2yl}carbonyl)cyclopentyl]amino}-4-O-methyl-D-erythro-pentitol;

1,5-Anhydro-2,3-dideoxy-3-{[(1R,3S)-3-(1-hydroxycyclobutyl)-3-({(1S,4S)-5-[6(trifluoromethyl)pyrazin-2-yl]-2,5-diazabicyclo[2.2.1]hept-2yl}carbonyl)cyclopentyl]amino}-4-O-methyl-D-erythro-pentitol;

1,5-Anhydro-2,3-dideoxy-3-{[(1 R,3S )-3-(1-hydroxycyclobutyl )-3-( {(1 S,4S )-5-(2(trifluoromethyl)pyridin-4-yl]-2,5-diazabicyclo[2.2.1]hept-2yl}carbonyl)cyclopentyl]amino}-4-O-methyl-D-erythro-pentitol;

1,5-Anhydro-2,3-dideoxy-3-{[3-(1-hydroxy-1-methylethyl)-3-({5-[4(trifluoromethyl)pyridin-2-yl]-2,5-diazabicyclo(2.2.1]hept-2yl}carbonyl)cyclopentyl]amino)-4-O-methylpentitol;

1,5-Anhydro-2,3-dideoxy-3-{[3-(1-hydroxy-1-methylethyl)-3-({5-[6(trifluoromethyl)pyrimidin-4-yl]-2,5-diazabicyclo[2.2.1]hept-2yl)carbonyl)cyclopentyl]amino}-4-O-methylpentitol;

1,5-Anhydro-2,3-dideoxy-3-{[3-(1-hydroxy-1-methylethyl)-3-({5-[4(trifluoromethyl)pyrimidin-2-yl]-2,5-diazabicyclo[2.2.1]hept-2yl}carbonyl)cyclopentyl]amino}-4-O-methylpentitol;

1,5-An hydro-2,3-did eoxy-3-{[3-( 1 -hyd roxy-1 -m ethyl ethyl )-3-( {5-[5(trifluoromethyl)pyridazin-3-yl]-2,5-diazabicyclo[2.2.1]hept-2yl)carbonyl)cyclopentyl]amino)-4-O-methylpentitol;

1,5-Anhydro-2,3-dideoxy-3-{[3-(1-hydroxy-1-methylethyl)-3-({5-[2(trifluoromethyl)pyrimidin-4-yl]-2,5-diazabicyclo[2.2.1]hept-2yl}carbonyl)cyclopentyl]amino}-4-O-methylpentitol;

1,5-Anhydro-2,3-dideoxy-3-{[3-(1-hydroxy-1-methylethyl)-3-({5-[6(trifluoromethyl)pyridin-2-yl]-2,5-diazabicyclo[2.2.1]hept-2yl}carbonyl)cyclopentyl]amino}-4-O-methylpentitol;

1,5-An hyd ro-2,3-d ideoxy-3-{[3-( 1 -hyd roxy-1 -methylethyl )-3-( {5-[2(trifluoromethyl)pyridin-4-yl]-2,5-diazabicyclo[2.2.1]hept-2yl}carbonyl)cyclopentyl]amino}-4-O-methylpentitol; and 1,5-Anhydro-2,3-dideoxy-3-{[3-(1-hydroxy-1-methylethyl)-3-({5-[6(trifluoromethyl)pyrazin-2-yl]-2,5-diazabicyclo[2.2.1]hept-2yl}carbonyl)cyclopentyl]amino)-4-O-methylpentitol.

In another embodiment, there is provided a compound of formula:

pharmaceutically acceptable sait thereof..

In another embodiment, there is provided a compound of formula

pharmaceutically acceptable sait thereof.

In another embodiment of the présent invention, there is provided a composition comprising a compound of Formula I or II, or a pharmaceutically acceptable sait thereof, and a carrier.

In another embodiment, there is provided a method of treating a disease 15 associated with expression or activity of a chemokine receptor in a patient comprising administering to said patient a compound of Formula I or II, or a pharmaceutically acceptable sait thereof.

In one embodiment of the method, said chemokine receptor is CCR2 or 20 CCR5.

In another embodiment of the method, the disease is rheumatoid arthritis, atherosclerosis, lupus, multiple sclerosis, pain, transplant rejection, diabètes, diabetic nephropathy, diabetic conditions, liver fibrosis, viral disease, cancer, asthma, seasonal and perennial allergie rhinitis, sinusitis, conjunctivitis, agerelated macular degeneration, food allergy, scombroid poisoning, psoriasis, undifferentiated spondyloarthropy, goût, urticaria, pruritus, eczema, inflammatory bowel disease, thrombotic disease, otitis media, fibrosis, liver cirrhosis, cardiac disease, Alzheimer's disease, sepsis, restenosis, Crohn’s disease, ulcerative colitis, irritable bowel syndrome, hypersensitivity lung diseases, drug-induced pulmonary fibrosis, chronic obstructive pulmonary disease, arthritis, nephritis, atopie dermatitis, stroke, acute nerve injury, sarcoidosis, hepatitis, endometriosis, neuropathie pain, hypersensitivity pneumonitis, éosinophilie pneumonies, delayed-type hypersensitivity, interstitial lung disease, eye disorders or obesity.

In anther embodiment of the présent invention, there is provided the use of a compound of Formula I or II, or a pharmaceutically acceptable sait thereof, in the manufacture of a médicament for the treatment of rheumatoid arthritis, atherosclerosis, lupus, multiple sclerosis, pain, transplant rejection, diabètes, diabetic nephropathy, diabetic conditions, liver fibrosis, viral disease, cancer, asthma, seasonal and perennial allergie rhinitis, sinusitis, conjunctivitis, agerelated macular degeneration, food allergy, scombroid poisoning, psoriasis, undifferentiated spondyloarthropy, goût, urticaria, pruritus, eczema, inflammatory bowel disease, thrombotic disease, otitis media, fibrosis, liver cirrhosis, cardiac disease, Alzheimer's disease, sepsis, restenosis, Crohn's disease, ulcerative colitis, irritable bowel syndrome, hypersensitivity lung diseases, drug-induced pulmonary fibrosis, chronic obstructive pulmonary disease, arthritis, nephritis, atopie dermatitis, stroke, acute nerve injury, sarcoidosis, hepatitis, endometriosis, neuropathie pain, hypersensitivity pneumonitis, éosinophilie pneumonias, delayed-type hypersensitivity, interstitial lung disease, eye disorders or obesity.

In another embodiment, the présent invention relates to a combination for treating a CCR2 or CCR2/CCR5 mediated disease, disorder or condition, said combination comprising a compound of Formula I or II as defined above, or a pharmaceutically acceptable sait thereof, and one or more additional therapeutic agents.

In another embodiment, the présent invention relates to a compound of formula I or II as defined above, or a pharmaceutically acceptable sait thereof, as defined above, for use as a médicament.

In another embodiment, the présent invention is directed to a method of treating a CCR2 or CCR2/CCR5 mediated disease, disorder or condition in a subject in need of such treatment, by administering a therapeutically effective amount of a compound of Formula I or II as defined above, or a pharmaceutically acceptable sait or solvaté thereof to said subject.

In another embodiment, the présent invention is directed to a compound of Formula I or II as defined above, or a pharmaceutically acceptable sait thereof, for use in treating a CCR2 or CCR2/CCR5 mediated disease, disorder or condition.

In another embodiment, the présent invention is directed to the use of a compound of Formula I or II as defined above, or a pharmaceutically acceptable sait or solvaté thereof, for the manufacture of a médicament for treating a CCR2 or CCR2/CCR5 mediated disease, disorder or condition.

Salts of compounds of the invention can include the acid addition salts or base addition salts (including disalts) thereof. The salts can be pharmaceutically acceptable.

Suitable acid addition salts are formed from acids which form non-toxic salts. Examples include the acetate, aspartate, benzoate, besylate, bicarbonate/carbonate, bisulphate/sulphate, borate, camsylate, citrate, edisylate, esylate, formate, fumarate, gluceptate, gluconate, glucuronate, hexafluorophosphate, hibenzate, hydrochloride/chloride, hydrobromide/bromide, hydroiodide/iodide, isethionate, lactate, malate, maleate, malonate, mesylate, methylsulphate, naphthylate, 2-napsylate, nicotinate, nitrate, orotate, oxalate, palmitate, pamoate, phosphate/hydrogen phosphate/dihydrogen phosphate, saccharate, stéarate, succinate, tartrate, tosylate and trifluoroacetate salts.

Suitable base salts are formed from bases which form non-toxic salts. Examples include the aluminium, arginine, benzathine, calcium, choline, diethylamine, diolamine, glycine, lysine, magnésium, meglumine, olamine, potassium, sodium, tromethamine and zinc salts.

Hemisalts of acids and bases may also be formed, for example, hemisulphate and hemicalcium salts.

For a review on suitable salts, see “Handbook of Pharmaceutical Salts: Properties, Sélection, and Use by Stahl and Wermuth (Wiley-VCH, Weinheim, Germany, 2002).

A sait may be readily prepared by mixing together solutions of compounds of the présent invention and the desired acid or base, as appropriate. The sait may precipitate from solution and be collected by filtration or may be recovered by évaporation of the solvent. The degree of ionization in the sait may vary from completely ionized to almost non-ionized.

The compounds of the présent invention may be administered as prodrugs. Thus, certain dérivatives which may hâve little or no pharmacological activity themselves can, when administered into or onto the body, be converted into compounds of the présent invention having the desired activity, for example, by hydrolytic cleavage. Such dérivatives are referred to as ‘prodrugs’. Further information on the use of prodrugs may be found in ‘Pro-drugs as Novel Delivery Systems, Vol. 14, ACS Symposium Sériés (T Higuchi and W Stella) and 'Bioreversible Carriers in Drug Design’, Pergamon Press, 1987 (ed. E B Roche, American Pharmaceutical Association).

Prodrugs can, for example, be produced by replacing appropriate functionalities présent In the compounds of the présent invention with certain moieties known to those skilled in the art as ‘pro-moieties’ as described, for example, in Design of Prodrugs by H Bundgaard (Elsevier, 1985).

Some examples of such prodrugs include:

(i) where the compound contains an alcohol functionality (-OH), an ether thereof, for example, replacement of the hydrogen with (CiC₆)alkanoyloxymethyl; and (ii) where the compound contains a secondary amino functionality , an amide thereof, for example, replacement of hydrogen with (Ci-Cio)alkanoyl. Ail isomers, such as stereoisomers, géométrie (c/s/trans or Z/E) isomers and tautomeric forms of the compounds or salts are included in the scope of the présent invention, including compounds or salts having more than one type of isomerism, and mixtures of one or more thereof.

Also included are acid addition or base salts wherein the counterion is optically active, for example, D-lactate or L-lysine, or racemic, for example, DLtartrate or DL-arginine.

Isomers may be separated by conventional techniques well known to those skilled in the art.

The présent invention includes isotopically-labelled compounds of the invention wherein one or more atoms are replaced by atoms having the same atomic number, but an atomic mass or mass number different from the atomic mass or mass number usually found in nature.

Isotopically-labeled compounds of the invention can generally be prepared by conventional techniques known to those skilled in the art or by processes analogous to those described in the accompanying Examples and Préparations using an appropriate isotopically-labelled reagent in place of the non-labeled reagent previously employed.

For the treatment of the conditions referred to below, the compounds of the présent invention can be administered. Salts of the compounds of the présent invention could also be used.

C. Compositions

Compounds or salts of the présent invention could be part of a composition. Compositions can also include one or more compounds or salts of the présent invention. The composition can also include an enantiomeric excess of one or more compounds of the présent invention. Other pharmacologically active substances and carriers can be included in the composition.

One embodiment is a composition comprising a compound of Formula I or II, or a sait thereof. Another embodiment is a composition comprising a compound of Formula I or II, or a sait thereof and a carrier.

For example, the carrier can be an excipient. The choice of excipient will to a large extent dépend on factors such as the particular mode of administration, the effect of the excipient on solubility and stability, and the nature of the dosage form.

The composition can be a solid, a liquid, or both, and may be formulated with the compound as a unit-dose composition, for example, a tablet, which can contain from 0.05% to 95% by weight of the active compounds. Compounds or salts of the présent invention may be coupled with suitable polymers as targetable drug carriers.

D. Methods

In some embodiments, compounds of the invention can be used in methods that modulate activity of one or more chemokine receptors. Accordingly, the invention includes methods comprising contacting a chemokine receptor with a compound of Formula I or II, or a sait thereof. In some embodiments, the chemokine receptor is CCR2. In other embodiments, the chemokine receptor is CCR5. In other embodiments, the invention includes methods of modulating a chemokine receptor by contacting the receptor with any one or more of the compounds or compositions described herein. In some embodiments, compounds of the présent invention can act as inhibitors or antagonist of chemokine receptors. In further embodiments, the compounds of

3l the invention can be used to modulate activity of a chemokine receptor in an individual in need of modulation of the receptor by administering a modulating amount of a compound of Formula I or II, or a slat thereof.

Chemokine receptors to which the présent compounds bind and/or modulate include any chemokine receptor. In some embodiments, the chemokine receptor belongs to the CC family of chemokine receptors including, for exampîe, CCR1, CCR2, CCR3, CCR4, CCR5, CCR6, CCR7, CCR8, and CCR10. In some embodiments, the chemokine receptor is CCR2 or CCR5. In other embodiments, the chemokine receptor is CCR2. In some embodiments, the chemokine receptor is CCR5. In some embodiments, the chemokine receptor binds and/or modulâtes both CCR2 and CCR5.

The compounds of the invention can be sélective, that is a compound binds to or inhibits a chemokine receptor with greater affinity or potency, respectively, compared to at least one other chemokine receptor.

Compounds of the invention can be dual inhibitors or binders of CCR2 and CCR5, meaning that the compounds of the invention can bind to or inhibit both CCR2 and CCR5 with greater affinity or potency, respectively, than for other chemokine receptors such as CCR1, CCR3, CCR4, CCR6, CCR7, CCR8, and CCR10. In some embodiments, the compounds of the invention hâve binding or inhibition selectivity for CCR2 and CCR5 over any other chemokine receptor. Binding affinity and inhibitor potency can be measured according to routine methods in the art, such as according to the assays provided herein.

The présent invention further provides methods of treating a chemokine receptor-associated disease or disorder in an individual (e.g., patient) by administering to the individual in need of such treatment a therapeutically effective amount or dose of a compound of the présent invention or a pharmaceutical composition thereof. In other embodiments, the invention includes method of treating a condition mediated by chemokine receptor activity in a subject comprising administering to the subject a compound of Formula I or II, or a pharmaceutically acceptable sait thereof.

A chemokine receptor-associated disease or condition can include any disease, disorder or condition that is directly or indirectly linked to expression or activity of the chemokine receptor. A chemokine receptor-associated disease or condition can also include any disease, disorder or condition that can be prevented, ameliorated, or cured by modulating chemokine receptor activity. A chemokine receptor-associated disease can further include any disease, disorder or condition that is characterized by binding of an infectious agent such as a virus or viral protein with a chemokine receptor. In some embodiments, the chemokine receptor-associated disease is a CCR5-associated disease such as HIV infection.

Examples of a condition mediated by chemokine receptor include inflammation, inflammatory diseases, immune disorders, pains, cancers, or viral infections.

Examples of a condition mediated by chemokine receptor include inflammation, inflammatory diseases, immune disorders, pains, cancers, or viral infections.

Examples of inflammatory diseases include diseases believed to hâve an inflammatory component such as asthma, seasonal and perennial allergie rhinitis, sinusitis, conjunctivitis, age-related macular degeneration, food allergy, scombroid poisoning, psoriasis, undifferentiated spondyloarthropy, juvenile-onset spondyloarthritis, goût, urticaria, pruritus, eczema, inflammatory bowel disease, thrombotic disease, otitis media, fibrosis, liver fibrosis, liver cirrhosis, cardiac disease, Alzheimer's disease, sepsis, restenosis, atherosclerosis, multiple sclerosis, Crohn's disease, ulcerative colitis, irritable bowel syndrome, inflammatory bowel disease, hypersensitivity lung diseases, drug-induced pulmonary fibrosis, chronic obstructive pulmonary disease (COPD), arthritis, nephritis, ulcerative colitis, atopie dermatitis, stroke, acute nerve injury, sarcoidosis, hepatitis, endometriosis, neuropathie pain, hypersensitivity pneumonitis, éosinophilie pneumonias, delayed-type hypersensitivity, interstitial lung disease (ILD) (e.g., idiopathic pulmonary fibrosis, or ILD associated with rheumatoid arthritis, systemic lupus erythematosus, ankylosing spondylitis, systemic sclerosis, Sjogren's syndrome, polymyositis or dermatomyositîs, eye disorders (e.g., retinal neurodegeneration, choroidal neovascularization, etc.) and the like.

Example immune disorders include rheumatoid arthritis, psoriatic arthritis, systemic lupus erythematosus, myastenia gravis, juvénile onset diabètes; giomerulonephritis, autoimmune throiditis, organ transplant rejection including allograft rejection and graft-versus-host disease.

Examples of pain include nociceptive and neuropathie pain. The pain can be acute or chronic. Pain includes cutaneous pain, somatic pain, viscéral pain, and phantom limb pain. Pain also includes fibromyalgia, rheumatoid arthritis pain, osteoarthritis pain, and pain associated with the other diseases and conditions detailed herein.

Example cancers include breast cancer, ovarian cancer, multiple myeloma and the like that are characterized by infiltration of macrophages (e.g., tumor associated macrophages, TAMs) into tumors or diseased tissues.

Example viral infections include influenza, avian influenza, herpes infection, HIV infection or AIDS.

Further inflammatory or immune diseases treatable by administration of a compound of the présent invention include, for example, autoimmune nephritis, lupus nephritis, Goodpasture's syndrome nephritis and Wegeners granulomatosis nephritis, lupus erythematosus, Goodpasture’s syndrome and Wegeners granulomatosis.

Examples of diabetic conditions include diabètes, insulin résistance, diabetic neuropathy, diabetic nephropathy, diabetic retinopathy, cataracts, hyperglycemia, hypercholesterolemia, hypertension, hyperinsulinemia, hyperlipidemia, atherosclerosis, tissue ischemia, diabetic cardiomyopathy, diabetic microangiopathy .diabetic macroangiopathy and foot ulcers. Included in the treatment of diabètes is the prévention or atténuation of long term conditions such as neuropathy, nephropathy, retinopathy or cataracts.

In some embodiments, the condition to be treated is rheumatoid arthritis, atherosclerosis, lupus, multiple sclerosis, neuropathie pain, transplant rejection, diabètes, diabetic nephropathy, diabetic conditions, or obesity.

In some embodiments, the condition is rheumatoid arthritis.

In some embodiments, the condition is diabètes.

In some embodiments, the condition is diabetic nephropathy.

In some embodiments, the condition is liver fibrosis.

In some embodiments, the condition is osteoarthritis pain.

In some embodiments, the condition is breast cancer, ovarian cancer or multiple myeloma.

In some embodiments, the condition is HIV infection.

E. Dosage and Administration

Pharmaceutical Formulations and Dosage Forms

When employed as pharmaceuticals, the compounds of Formula I or II can be administered in the form of pharmaceutical compositions. These compositions can be prepared in a manner well known in the pharmaceutical art, and can be administered by a variety of routes depending upon whether local or systemic treatment is desired and upon the area to be treated. Administration can be topical (including ophthalmic and to mucous membranes including intranasal, vaginal and rectal delivery), pulmonary (e.g., by inhalation or insufflation of powders or aérosols, including by nebulizer; intratracheal, intranasal, epidermal and transdermal), oral or parentéral. Parentéral administration includes intravenous, intraarterial, subeutaneous, intraperitoneal intramuscular or injection or infusion; or intracranial, e.g., intrathecal or intraventricular, administration. Parentéral administration can be in the form of a single bolus dose, or can be, for example, by a continuous perfusion pump. Pharmaceutical compositions and formulations for topical administration can include transdermal patches, ointments, lotions, creams, gels, drops, suppositories, sprays, liquids and powders. Conventional pharmaceutical carriers, aqueous, powder or oily bases, thickeners and the like may be necessary or désirable. Coated condoms, gloves and the like may also be useful.

This invention also includes pharmaceutical compositions which contain, as the active ingrédient, one or more of the compounds of Formula I or II above in combination with one or more pharmaceutically acceptable carriers. In making the compositions of the invention, the active ingrédient is typically mixed with an excipient, diluted by an excipient or enclosed within such a carrier in the form of, for example, a capsule, sachet, paper, or other container. When the excipient serves as a diluent, it can be a solid, semi-solid, or liquid material, which acts as a vehicle, carrier or medium for the active ingrédient. Thus, the compositions can be in the form of tablets, pills, powders, lozenges, sachets, cachets, élixirs, suspensions, émulsions, solutions, syrups, aérosols (as a solid or in a liquid medium), ointments containing, for example, up to 10% by weight of the active compound, soft and hard gelatin capsules, suppositories, stérile injectable solutions, and stérile packaged powders.

In preparing a formulation, the active compound can be milled to provide the appropriate particle size prior to combining with the other ingrédients. If the active compound is substantially insoluble, it can be milled to a particle size of less than 200 mesh. If the active compound is substantially water soluble, the particle size can be adjusted by milling to provide a substantially uniform distribution in the formulation, e.g. about 40 mesh.

Some examples of suitable excipients include lactose, dextrose, sucrose, sorbitol, mannitol, starches, gum acacia, calcium phosphate, alginates, tragacanth, gelatin, calcium silicate, microcrystalline cellulose, polyvinylpyrrolidone, cellulose, water, syrup, and methyl cellulose. The formulations can additionally include: lubricating agents such as talc, magnésium stéarate, and minerai oil; wetting agents; emulsifying and suspending agents; preserving agents such as methyl- and propylhydroxy-benzoates; sweetening agents; and flavoring agents. The compositions of the invention can be formulated so as to provide quick, sustained or delayed release of the active ingrédient after administration to the patient by employing procedures known in the art.

The compositions can be formulated in a unit dosage form, each dosage containing from about 5 to about 1000 mg (1 g), more usually about 100 to about 500 mg, of the active ingrédient. The term unit dosage forms refers to physically discrète units suitable as unitary dosages for human subjects and other mammals, each unit containing a predetermined quantity of active material calculated to produce the desired therapeutic effect, in association with a suitable pharmaceutical excipient.

In some embodiments, the compounds or compositions of the invention contain from about 5 to about 50 mg of the active ingrédient. One having ordinary skill in the art will appreciate that this embodies compounds or compositions containing from about 5 to about 10, from about 10 to about 15, from about 15 to about 20, from about 20 to about 25, from about 25 to about 30, from about 30 to about 35, from about 35 to about 40, from about 40 to about 45, or from about 45 to about 50 mg of the active ingrédient. In another embodiment, the dose can be 35-45 mg.

In some embodiments, the compounds or compositions of the invention contain from about 50 to about 500 mg of the active ingrédient. One having ordinary skill in the art will appreciate that this embodies compounds or compositions containing from about 50 to about 75, from about 75 to about 100, from about 100 to about 125, from about 125 to about 150, from about 150 to about 175, from about 175 to about 200, from about 200 to about 225, from about 225 to about 250, from about 250 to about 275, from about 275 to about 300, from about 300 to about 325, from about 325 to about 350, from about 350 to about 375, from about 375 to about 400, from about 400 to about 425, from about 425 to about 450, from about 450 to about 475, or from about 475 to about 500 mg of the active ingrédient.

In some embodiments, the compounds or compositions of the invention contain from about 500 to about 1000 mg of the active ingrédient. One having ordinary skill in the art will appreciate that this embodies compounds or compositions containing from about 500 to about 550, from about 550 to about 600, from about 600 to about 650, from about 650 to about 700, from about 700 to about 750, from about 750 to about 600, from about 800 to about 850, from about 850 to about 900, from about 900 to about 950, or from about 950 to about 1000 mg of the active ingrédient.

The active compound can be effective over a wide dosage range and is generally administered in a pharmaceutically effective amount. It will be understood, however, that the amount of the compound actually administered will usually be determined by a physician, according to the relevant circumstances, including the condition to be treated, the chosen route of administration, the actual compound administered, the âge, weight, and response of the individual patient, the severity of the patient's symptoms, and the like.

For preparing solid compositions such as tablets, the principal active ingrédient is mixed with a pharmaceutical excipient to form a solid preformulation composition containing a homogeneous mixture of a compound of the présent invention. When referring to these preformulation compositions as homogeneous, the active ingrédient is typically dispersed evenly throughout the composition so that the composition can be readily subdivided into equally effective unit dosage forms such as tablets, pills and capsules. This solid preformulation is then subdivided into unit dosage forms of the type described above containing from, for example, 0.1 to about 1000 mg of the active ingrédient of the présent invention.

The tablets or pills of the présent invention can be coated or otherwise compounded to provide a dosage form affording the advantage of prolonged action. For example, the tablet or pill can comprise an tnner dosage and an outer dosage component, the latter being in the form of an envelope over the former. The two components can be separated by an enteric layer which serves to resist disintegration in the stomach and permit the inner component to pass intact into the duodénum or to be delayed in release. A variety of materials can be used for such enteric layers or coatings, such materials including a number of polymeric acids and mixtures of polymeric acids with such materials as shellac, cetyl alcohol, and cellulose acetate.

The liquid forms in which the compounds and compositions of the présent invention can be incorporated for administration orally or by injection include aqueous solutions, suitably flavored syrups, aqueous or oil suspensions, and flavored émulsions with edible oils such as cottonseed oil, sesame oil, coconut oil, or peanut oil, as well as élixirs and similar pharmaceutical vehicles.

Compositions for inhalation or insufflation include solutions and suspensions in pharmaceutically acceptable, aqueous or organic solvents, or mixtures thereof, and powders. The liquid or solid compositions may contain suitable pharmaceutically acceptable excipients as described supra. In some embodiments, the compositions are administered by the oral or nasal respiratory route for local or systemic effect. Compositions in can be nebulized by use of inert gases. Nebulized solutions may be breathed directly from the nebulizing device or the nebulizing device can be attached to a face masks tent, or intermittent positive pressure breathing machine. Solution, suspension, or powder compositions can be administered orally or nasally from devices which deliver the formulation in an appropriate manner.

The amount of compound or composition administered to a patient will vary depending upon what is being administered, the purpose of the administration, such as prophylaxis or therapy, the state of the patient, the manner of administration, and the like. In therapeutic applications, compositions can be administered to a patient already suffering from a disease in an amount sufficient to cure or at least partially arrest the symptoms of the disease and its complications. Effective doses will dépend on the disease condition being treated as well as by the judgment of the attending clinician depending upon factors such as the severity of the disease, the âge, weight and general condition of the patient, and the like.

The compositions administered to a patient can be in the form of pharmaceutical compositions described above. These compositions can be sterilized by conventional sterilization techniques, or may be stérile filtered.

Aqueous solutions can be packaged for use as is, or lyophilized, the lyophilized préparation being combined with a stérile aqueous carrier prior to administration. The pH of the compound préparations typically will be between 3 and 11, or from 5 to 9, or from 7 to 8. It will be understood that use of certain of the foregoing excipients, carriers, or stabilizers will resuit in the formation of pharmaceutical salts.

The therapeutic dosage of the compounds of the présent invention can vary according to, for example, the particular use for which the treatment is made, the manner of administration of the compound, the health and condition of the patient, and the judgment of the prescribing physician. The proportion or concentration of a compound of the invention in a pharmaceutical composition can vary depending upon a number of factors including dosage, Chemical characteristics (e.g., hydrophobicity), and the route of administration. For example, the compounds of the invention can be provided in an aqueous physiological buffer solution containing about 0.1 to about 10% w/v of the compound for parentéral adminstration. Some typical dose ranges are from about 1 pg/kg to about 1 g/kg of body weight per day. In some embodiments, the dose range is from about 0.01 mg/kg to about 100 mg/kg of body weight per day. The dosage is likely to dépend on such variables as the type and extent of progression of the disease or disorder, the overall health status of the particular patient, the relative biological efficacy of the compound selected, formulation of the excipient, and its route of administration. Effective doses can be extrapolated from dose-response curves derived from in vitro or animal model test Systems.

The compounds of the invention can also be formulated in combination with one or more additional active ingrédients which can include any pharmaceutical agent such as antibodies, immune suppressants, antiinflammatory agents, chemotherapeutics, lipid lowering agents, HDL elevating agents, insulin secretagogues or sensitizers, drugs used for the treatment of rheumatoid arthritis and the like.

Rheumatoid Arthritis (RA) Treatment Regimen

Rheumatoid arthritis (RA) patients, treated aggressively with disease modifying agents (methotrexate, antimalarials, gold, penicillamine, sulfasalazine, dapsone, leflunamide, or biologicals), can achieve varying degrees of disease control, including complété remissions. These clinical responses are associated with improvement in standardized scores of disease activity, specifically the ACR criteria which includes: pain, function, number oftender joints, number of swollen joints, patient global assessment, physician global assessment, laboratory measures of inflammation (CRP and ESR), and radiologie assessment of joint structural damage. Current disease-modifying drugs (DMARDs) require continued administration to maintain optimal benefit. Chronic dosing of these agents is associated with significant toxicity and host defense compromise. Additionally, patients often become refractory to a particular therapy and require an alternative regimen. For these reasons, a novel, effective therapy which allows withdrawal of standard DMARDs would be a clinically important advance.

Patients with significant response to anti-TNF thérapies (infliximab, etanercept, adalimumab), anti- IL-1 therapy (kinaret) or other disease modifying anti-rheumatic drugs (DMARDs) including but not limited to methotrexate, cyclosporine, gold salts, antimalarials, penicillamine or leflunamide, who hâve achieved clinical remission of disease can be treated with a substance that inhibits expression and/or activity of CCR2 including, for example, nucleic acids (e.g., antisense or siRNA molécules), proteins (e.g., anti-CCR2 antibodies), small molécule inhibitors (e.g., the compounds disclosed herein and other chemokine receptor inhibitors known in the art).

In some embodiments, the substance that inhibits expression and/or activity of CCR2 is a small molécule CCR2 inhibitor (or antagonist). The CCR2 antagonist can be dosed orally q.d. or b.i.d at a dose not to exceed about 500 mgs a day. The patients can be withdrawn from or hâve a decrease in the dosage of their current therapy and would be maintained on treatment with the CCR2 antagonist. Treating patients with a combination of CCR2 antagonist and their current therapy can be carried out for, for example, about one to about two

4l days, before discontinuing or dose reducing the DMARD and continuing on CCR2 antagonist.

Advantages of substituting traditional DMARDS with CCR2 antagonists are numerous. Traditional DMARDs hâve serious cumulative dose-limiting side effects, the most common being damage to the liver, as well as immunosuppressive actions. CCR2 antagonism is expected to hâve an improved long-term safety profile and will not hâve similar immunosuppressive liabilities associated with traditional DMARDs. Additionally, the half-life of the biologicals is typically days or weeks, which is an issue when dealing with adverse reactions. The half-life of an orally bioavailable CCR2 antagonist is expected to be on the order of hours so the risk of continued exposure to the drug after an adverse event is very minimal as compared to biological agents. Also, the current biologie agents (infliximab, etanercept, adalimumab, kinaret) are typically given either i.v. or s.c., requiring doctor’s administration or patient selfinjection. This leads to the possibility of infusion reaction or injection site reactions. These are avoidable using an orally administered CCR2 antagonist.

Diabètes and Insulin Résistance Treatment Regimen

Type 2 diabètes is one of the leading causes of morbidity and mortality in western societies. In the vast majority of patients, the disease is characterized by pancreatic beta-cell dysfunction accompanied by insulin résistance in the liver and in peripheral tissues. Based on the primary mechanisms that are associated with disease, two general classes of oral thérapies are available to treat type 2 diabètes: insulin secretagogues (sulfonylureas such as glyburide) and insulin sensitizers (metformin and thiazolidinediones such as rosiglitazone). Combination therapy that addresses both mechanisms has been shown to manage the metabolic defects of this disease and in many instances can be shown to ameliorate the need for exogenous insulin administration. However, with time, insulin résistance often progresses, leading to the need for further insulin supplémentation. In addition, a prediabetic State, referred to as the metabolic syndrome, has been demonstrated to be characterized by impaired glucose tolérance, particularly in association with obesity. The majority of patients who develop type 2 diabètes begin by developing insulin résistance, with the hyperglycemia occurring when these patients can no longer sustain the degree of hyperinsulinemia necessary to prevent loss of glucose homeostasis. The onset of the insulin résistance component is highly prédictive of disease onset and is associated with an increase in the risk of developing type 2 diabètes, hypertension and coronary heart disease.

One of the strongest correlates of impaired glucose tolérance and of the progression from an insulin résistant State to type 2 diabètes is the presence of central obesity. Most patients with type 2 diabètes are obese and obesity itself is associated with insulin résistance. It is clear that central adiposity is a major risk factor for the development of insulin résistance leading to type 2 diabètes, suggesting that signais from viscéral fat contribute to the development of insulin résistant and progression to disease. In addition to the secreted protein factors, obesity induces a cellular inflammatory response in which bone-marrow derived macrophages accumulate in adipose depots, becoming adipose tissue macrophages. Adipose tissue macrophages accumulate in adipose tissue in proportion to measures of adiposity. Tissue infiltrating macrophages are a source of many of the inflammatory cytokines that hâve been demonstrated to induce insulin résistance in adipocytes.

Adipose tissue produces MCP-1 in proportion to adiposity, suggesting that its activity by signaling through CCR2 also might play an important rôle in the accumulation of macrophages in adipose tissue. It is unknown whether the MCP-1/CCR2 interaction is directly responsible for monocyte recruitment to adipose tissue, whether reduced recruitment of macrophages to adipose tissue in humans will directly lead to the reduced production of proinflammatory molécules and whether the proinflammatory molécule production is directly linked to insulin résistance.

Patients who demonstrate insulin résistance, either prediabetic (normoglycemic) or diabetic (hyperglycémie), could be treated with a substance that inhibits the expression and/or activity of CCR2 including, for example, nucleic acids (e.g., antisense or siRNA molécules), proteins (e.g., anti-CCR2 antibodies), small molécule inhibitors (e.g., the compounds disclosed herein and other chemokine receptor inhibitors known in the art). In some embodiments, the substance that inhibits expression and/or activity of CCR2 is a small molécule CCR2 inhibitor (or antagonist). The CCR2 antagonist can be dosed orally q.d. or b.i.d. The patients can be withdrawn from or hâve a decrease in the dosage of their current therapy and would be maintained on treatment with the CCR2 antagonist. Alternately CCR2 antagonist treatment may be used to supplément their current therapy to enhance its effectiveness or to prevent progression to further insulin dependence.

Advantages of substituting or supplementing traditional agents with CCR2 antagonists are numerous. Such agents may be useful, for example, to preclude progression from a prediabetic, insulin résistant State to a diabetic State. Such agents may reduce or replace the need for the use of insulin sensitizers, with their attendant toxicities. Such agents may also reduce the need for, or prolong the period untîl, exogenous insulin supplémentation is required.

Atherosclerosis Treatment Regimen

Atherosclerosis is a condition characterized by the déposition of fatty substances in arterial walls. Plaque encompasses such deposits of fatty substances, cholestérol, cellular waste products, calcium and other substances that build up in the inner lining of an artery. Plaques can grow large enough to significantly reduce the blood's flow through an artery. However, more significant damage occurs when the plaque becomes unstable and ruptures. Plaques that rupture cause blood clots to form that can block blood flow or break off and travel to other parts of the body. If the clôt blocks a blood vessel that feeds the heart, it causes a heart attack. If it blocks a blood vessel that feeds the brain, it causes a stroke. Atherosclerosis is a slow, complex disease that typically starts in childhood and often progresses as people grow older.

A high level of cholestérol in the blood is a major risk factor for coronary heart disease. Based on cholestérol as a primary composition of plaque, the advance of plaque formation has been managed by the réduction of circulating cholestérol or by élévation of cholesterol-carrying high density lipoproteins (HDL). Circulating cholestérol can be reduced, for example, by inhibiting its synthesis in the liver using or by reducing update from food. Such médicaments that act through these mechanism may include medicines that are used to lower high cholestérol levels: bile acid absorbers, lipoprotein synthesis inhibitors, cholestérol synthesis inhibitors and fibric acid dérivatives. Circulating HDL can additionally be elevated by administration of, for example, probuchol or high doses of niacin. Therapy that addresses multiple mechanisms has been shown to slow disease progression and progression to plaque rupture.

Atherosclerosis is typically accompanied by a cellular inflammatory response in which bone-marrow derived macrophages accumulate in fatty streaks along the vessel wall, becoming foam cells. Foam cells are a source of many of the inflammatory cytokines that hâve been demonstrated to induce plaque progression and of the enzymes that can promote plaque destabilization. Atherosclerotic tissue also produces MCP-1, suggesting that its activity by signaling through CCR2 also might play an important rôle in the accumulation of macrophages as foam cells in plaques. CCR2-/- mice hâve been demonstrated to hâve significantly reduced macrophages in fatty streaks generated as a resuit of high fat diet or genetic alteration in lipid metabolism.

Patients who demonstrate high circulating cholestérol, low HDL, or elevated circulating CRP or présent with vessel wall plaque by imaging, or any other evidence of the presence of atherosclerosis could be treated with a substance that inhibits the expression and/or activity of CCR2 including, for example, nucleic acids (e.g., antisense or siRNA molécules), proteins (e.g., antiCCR2 antibodies), small molécule inhibitors (e.g., the compounds disclosed herein and other chemokine receptor inhibitors known in the art). In some embodiments, the substance that inhibits expression and/or activity of CCR2 is a small molécule CCR2 inhibitor (or antagonist) such as a compound of the invention. The CCR2 antagonist can be dosed orally q.d. or b.i.d at a dose not to exceed about 500 mgs a day. The patients can be withdrawn from or hâve a decrease in the dosage of their current therapy and would be maintaîned on treatment with the CCR2 antagonist. Alternately CCR2 antagonist treatment may be used to supplément their current therapy to enhance its effectiveness in, for example, preventing plaque progression, stabilizing plaque that has already formed or inducing plaque régression.

Advantages of substituting or supplementing traditional agents with CCR2 antagonists are numerous. Such agents may be useful, for example, to preclude progression of the plaque to a stage of instability with its associated risk of plaque rupture. Such agents may reduce or replace the need for the use of cholestérol modifying drugs or HDL elevating drugs, with their attendant toxicities including, but not limited to, flushing, liver damage and muscle damage such as myopathy. Such agents may also reduce the need for, or prolong the period until, surgery is required to open the vessel wall or until use of anticoagulants is required to limit damage due to potential plaque rupture.

Labeled Compounds and Assay Methods

Another aspect of the présent invention relates to fluorescent dye, spin labié, heavy métal or radio-labeled compounds of Formula I that would be useful not only in imaging but also in assays, both in vitro and in vivo, for localizing and quantitating the chemokine receptor in tissue samples, including human, and for identifying chemokine receptor ligands by inhibition binding of a labeled compound. Accordingly, the présent invention includes chemokine receptor assays that contain such labeled compounds.

The présent invention further includes isotopically-labeled compounds of Formula I. An “isotopically or “radio-labeled compound is a compound of the invention where one or more atoms are replaced or substituted by an atom having an atomic mass or mass number different from the atomic mass or mass number typically found in nature (i.e., naturally occurring). Suitable radionuclides that may be incorporated in compounds of the présent invention include but are not limited to ²H, ³H (also written as T for tritium), ¹¹C, ¹³C, ¹⁴C, ¹³N, ¹⁵N, ¹⁵0,¹⁷O, ¹⁸0,¹⁸F, ³⁵S, ³⁶CI, ⁸²Br, ^7SBr, ⁷⁶Br, ⁷⁷Br, ¹²³l, ¹²⁴l, ¹²⁵i and ¹³¹1. The radionuclide that is incorporated in the instant radio-labeled compounds will dépend on the spécifie application of that radio-labeled compound. For example, for in vitro chemokine receptor labeling and compétition assays, compounds that incorporate ³H, ¹⁴C, ^0zBr, ¹²⁵l , ¹³¹1, or ³⁵S will generally be most useful. For radioimaging applications ¹¹C, ¹⁸F, ¹²⁵l, ¹²³l, ¹²⁴l, ¹³¹l, ⁷⁵Br, ⁷⁶Br or ⁷⁷Br will generally be most useful.

It is understood that a “radio-labeled or labeled compound” is a compound that has incorporated at least one radionuclide. In some embodiments the radionuclide is selected from the group consisting of ³H, ¹⁴C, ¹²⁵l, ³⁵S and ⁸²Br.

Synthetic methods for incorporating radio-isotopes into organic compounds are applicable to compounds of the invention and are well known in the art. A radio-labeled compound of the invention can be used in a screening assay to identify/evaluate compounds. In general terms, a newly synthesized or identified compound (i.e., test compound) can be evaluated for its ability to reduce binding of the radio-labeled compound of the invention to the chemokine receptor. Accordingly, the ability of a test compound to compete with the radiolabeled compound for binding to the chemokine receptor directly correlates to its binding affinity.

Kits

The présent invention also includes pharmaceutical kits useful, for example, in the treatment or prévention of chemokine-associated diseases which include one or more containers containing a pharmaceutical composition comprising a therapeutically effective amount of a compound of Formula I. Such kits can further include, if desired, one or more of various conventional pharmaceutical kit components, such as, for example, containers with one or more pharmaceutically acceptable carriers, additional containers, etc., as will be readily apparent to those skilled in the art. Instructions, either as inserts or as labels, indicating quantities of the components to be administered, guidelines for administration, and/or guidelines for mixing the components, can also be included in the kit.

Combination

The compounds or salts of the invention, or mixtures thereof, may be administered in combination with one or more other therapeutic agents, such as a drug. The compound of the présent invention or sait thereof may be administered at the same time or different time as one or more other therapeutic agents.

For example, in combination includes: simultaneous administration of a combination of compound or sait of the invention and a therapeutic agent to a subject, when such components are formulated together into a single dosage form which releases said components at substantially the same time to said subject; substantially simultaneous administration of a combination of compound or sait of the invention and a therapeutic agent to a subject in need of treatment, when such components are formulated apart from each other into separate dosage forms which are taken at substantially the same time by said subject, whereupon said components are released at substantially the same time to said subject; sequential administration of a combination of compound or sait of the invention and a therapeutic agent to a subject, when such components are formulated apart from each other into separate dosage forms which are taken at consecutive times by said subject with a significant time interval between each administration, whereupon said components are released at substantially different times to said subject; and sequential administration of such combination of compound or sait of the invention and a therapeutic agent to a subject, when such components are formulated together into a single dosage form which releases said components in a controlled manner whereupon they are concurrently, consecutively, and/or overlappingly administered at the same and/or different times by said subject, where each part may be administered by either the same or different route.

One or more additional pharmaceutical agents such as, for example, antibodies, anti-inflammatory agents, immunosuppressants, chemotherapeutics can be used in combination with the compounds of the présent invention for treatment of chemokine receptor-associated diseases, disorders or conditions.

One or more additional pharmaceutical agents such as, for example, antiviral agents, antibodies, anti-inflammatory agents, insulin secretagogues and sensitizers, sérum lipid and lipid-carrier modulating agents, and/or immunosuppressants can be used in combination with the compounds of the présent invention for treatment of chemokine receptor-associated diseases, disorders or conditions. The agents can be combined with the présent compounds in a single or continuous dosage form, or the agents can be administered simultaneously or sequentially as separate dosage forms.

Suitable antiviral agents contemplated for use in combination with the compounds of the présent invention can comprise nucleoside and nucléotide reverse transcriptase inhibitors (NRTIs), non-nucleoside reverse transcriptase inhibitors (NNRTIs), protease inhibitors and other antiviral drugs.

Suitable antiviral agents contemplated for use in combination with the compounds of the présent invention can comprise nucleoside and nucléotide reverse transcriptase inhibitors (NRTIs), non-nucleoside reverse transcriptase inhibitors (NNRTIs), protease inhibitors, entry inhibitors, fusion inhibitors, maturation inhibitors, and other antiviral drugs.

Example suitable NRTIs include zidovudine (AZT); didanosine (ddl); zalcitabine (ddC); stavudine (d4T); lamivudine (3TC); abacavir (1592U89); adefovir dipivoxil [bis(POM)-PMEAj; lobucavir (BMS-180194); BCH-10652; emitricitabine [(-)-FTC]; beta-L-FD4 (also called beta-L-D4C and named beta-L2', 3'-dicleoxy-5-fluoro-cytidene); DAPD, ((-)-beta-D-2,6,-diamino-purine dioxolane); and lodenosine (FddA).

Typical suitable NNRTIs include nevirapine (BI-RG-587); delaviradine (BHAP, U-90152); efavirenz (DMP-266); PNU-142721; AG-1549; MKC-442 (1(ethoxy-methyl)-5-(1 -methylethyl)-6-(phenylmethyl )-(2,4(1 H, 3H)-pyrimidi nedione); and (+)-calanolide A (NSC-675451) and B.

Typical suitable protease inhibitors include saquinavir (Ro 31-8959); ritonavir (ABT-538); indinavir (MK-639); nelfnavir (AG-1343); amprenavir (141W94); lasinavir (BMS-234475); DMP-450; BMS-2322623; ABT-378; and AG1 549.

Other antiviral agents include hydroxyurea, ribavirin, IL-2, IL-12, pentafuside, enfuvirtide, C-34, the cyclotriazadisulfonamide CADA, PA-457 and Yissum Project No. 11607.

In some embodiments, anti-inflammatory or analgésie agents contemplated for use in combination with the compounds of the présent invention can comprise, for example, an opiate agonist, a lipoxygenase inhibitor such as an inhibitor of 5-lipoxygenase, a cyclooxygenase inhibitor such as a cyclooxygenase-2 inhibitor, an interleukin inhibitor such as an interleukin-1 inhibitor, a TNF inhibitor such as infliximab, etanercept, or adalimumab an NNMA antagonist, an inhibitor of nitric oxide or an inhibitor of the synthesis of nitric oxide, a non-steroidal antiinflammatory agent, or a cytokine-suppressing antiinflammatory agent, for example, such as acetaminophen, aspirin, codeine, fentanyl, ibuprofen, indomethacin, ketodolac, morphine, naproxen, phenacetin, piroxicam, a steroidal analgésie, sufentanyl, sunlindac, tenidap, and the like. Similarly, the instant compounds can be administered with a pain reliever; a potentiator such as caffeine, an H2-antagonist, simethicone, aluminum or magnésium hydroxide; a decongestant such as phenylephrine, phenylpropanolamine, pseudophedrine, oxymetazoline, ephinephrine, naphazoline, xylometazoline, propylhexedfine, or levo-desoxyephedrine; an antfitussive such as codeine, hydrocodone, caramiphen, carbetapentane, or dextramethorphan; a diuretic; and a sedating or non-sedating antihistamine.

In some embodiments, pharmaceutical agents contemplated for use in combination with the compounds of the présent invention can comprise but are not limited to (a) VLA-4 antagonists such as those described in US 5,510,332, W095/15973, W096/01644, W096/06108, W096/20216, W096/229661, W096/31206, W096/4078, W097/030941, W097/022897 WO 98/426567 W098/53814, W098/53817, W098/538185, W098/54207, and W098/58902; (b) steroids such as beclornethasone, methylpi-ednisolone, betarnethasone, prednisone, dexamethasone, and hydrocortisone; (c) immunosuppressants such as cyclosporin, tacrolimus, raparnycin and other FK506 type immunosuppressants; (d) antihistamines (Hl-histamine antagonists) such as bromopheniramine, chlorpheniramine, dexchlorphenîramine, triprolidine, clemastine, diphenhydramine, diphenylpyraline, tripelennamine, hydroxyzine, methdilazine, promethazine, trimeprazine, azatadine, cyproheptadine, antazoline, pheniramine pyrilarnine, asternizole, terfenadine, loratadine, cetirizine, fexofenadine, desearboethoxyloratadine, and the like; (e) non-steroidal antiasthmatics such as terbutaline, metaproterenol, fenoterol, isoethaiine, albuterol, bitolterol, pirbuterol, theophylline, cromolyn sodium, atropine, ipratropium bromide, leukotriene antagonists (e.g., zafirlukast, montelukast, pranlukast, iralukast, pobilukast, SKB-106,203), leukotriene biosynthesis inhibitors (e.g., zileuton, BAY-1005); (f) nonsteroidal antiinflammatory agents (NSAIDs) such as propionic acid dérivatives (e.g., alminoprofen, benoxaprofen, bucloxic acid, carprofen, fenbufen, fenoprofen, fluprofen, flurbiprofen, ibuprofen, indoprofen, ketoprofen, miroprofen, naproxen, oxaprozin, pirprofen, pranoprofen, suprofen, tiaprofenic acid, and tioxaprofen), acetic acid dérivatives (e.g., indomethacin, acernetacin, alclofenac, clidanac, diclofenac, fenclofenac, fenclozic acid, fentiazac, furofenac, ibufenac, isoxepac, oxpinac, sulindac, tiopinac, tolmetin, zidometacin, and zomepirac), fenarnic acid dérivatives (flufenarnic acid, meclofenamic acid, rnefenamic acid, niflumic acid and tolfenarnic acid), biphenylcarboxylic acid dérivatives (diflunisal and flufenisal), oxicarns (isoxicarn, piroxicam, sudoxicam and tenoxican), salicylates (acetyl salicylic acid, sulfasalazine) and the pyrazolones (apazone, bezpiperylon, feprazone, mofebutazone, oxyphenbutazone, phenylbutazone); (g) cyclooxygenase-2 (COX2) inhibitors; (h) inhibitors of phosphodiesterase type IV (PDE-IV); (i) other antagonists of the chemokine receptors, especially CXCR-4, CCR1, CCR2, CCR3 and CCR5 ; G) cholestérol lowering agents such as HMG-CoA reductase inhibitors (lovastatin, sirrivastatin and pravastatin, fluvastatin, atorvastatin, and other statins), séquestrants (cholestyramine and colestipol), nicotinic acid,

5ΐ fenofibric acid dérivatives (gemfibrozil, clofibrat, fenofibrate and benzafibrate), and probucol; (k) anti-inflammatory biologie agents such as anti-TNF thérapies, anti-IL-1 receptor, CTLA-4lg, anti-CD20, and anti-VLA4 antibodies; (I) antidiabetic agents such as insulin, sulfonylureas, biguanides (metformin), U.glucosidase inhibitors (acarbose) and orlitazones (troglitazone and pioglitazone); (m) préparations of interferon beta (interferon beta- lo., interferon beta-1 P); (n) other compounds such as aminosalicylic acids, antimetabolites such as azathioprine and 6-mercaptopurine, and cytotoxic cancer chemotherapeutic agents. The weight ratio of the compound of the compound of the présent invention to the second active ingrédient may be varied and will dépend upon the effective dose of each ingrédient.

For example, a CCR2 and/or CCR5 antagonist can be used in combination with an anti-inflammatory pharmaceutical agent in the treatment of inflammation, metabolic disease, autoimmune disease, pain, cancer or viral infection to improve the treatment response as compared to the response to the therapeutic agent alone, without exacerbation of its toxic effects. Additive or synergistic effects are désirable outcomes of combining a CCR2 and/or CCR5 antagonist of the présent invention with an additional agent. Furthermore, résistance of cancer cells to agents such as dexamethasone can be réversible upon treatment with a CCR2 and/or CCR5 antagonist of the présent invention.

F. Use in the Préparation of a Composition or Médicament

In one embodiment, the présent invention comprises methods for the préparation of a composition or médicament comprising the compounds or salts of the présent invention for use in treating condition mediated by chemokine receptors.

In another embodiment, the invention comprises the use of one or more compounds or salts of the présent invention in the préparation of a composition or a médicament for inflammation, inflammatory disease, immune disorder, pain, cancer, or viral infection.

The présent invention also includes the use of one or more compounds or salts of the présent invention for préparation of a composition or a médicament for treating one or more conditions detailed in the Methods section.

G. Schemes

The compounds of the présent invention may be prepared using the methods illustrated in the general synthetic schemes and experimental procedures detailed below. The reactions of the synthetic methods herein are carried out in suitable solvents which may be readily selected by one skilled in the art of organic synthesis, said suitable solvents generally being any solvent which is substantially nonreactive with the starting materials (reactants), the intermediates, or products at the températures at which the reactions are carried out. A given reaction may be carried out in one solvent or a mixture of more than one solvent. Depending on the particular reaction step, suitable solvents for a particular reaction step may be selected.

Préparation of compounds of the invention can involve the protection and deprotection of various Chemical groups. The need for protection and deprotection, and the sélection of appropriate protecting groups can be readily determined by one skilled in the art. The chemistry of protecting groups can be found, for example, in T.W. Greene and P.G.M. Wuts, Protective Groups in Organic Synthesis, 3rd. Ed.₍ Wiley & Sons, Inc., New York (1999), which is incorporated herein by reference in its entirety.

Reactions can be monitored according to any suitable method known in the art. For example, product formation can be monitored by spectroscopic means, such as nuclear magnetic résonance spectroscopy (e.g., ¹H or ¹³C) infrared spectroscopy, spectrophotometry (e.g., UV-visible), or mass spectrometry, or by chromatography such as high performance liquid chromatography (HPLC) or thin layer chromatography.

The starting materials used herein are either commercially available or may be prepared by routine synthetic methods.

The general synthetic schemes are presented for purposes of illustration and are not intended to be limiting.

Scheme 1 Préparation of Examples 1-23

Schemel

a) LDA or NaHMDS, R-X, THF; b) 2.5N NaOH, MeOH; c) 2.2.1-Boc, DMF, DIPEA, Bop; d) H₂NOH*HCI, MeOH, NH₂OH(aq); e) 5% Pd/C. MeOH, 50 psi H₂; f) pyranone, DCM, Na(OAc)₃BH; g) 4N HC1/1,4-dioxane; h) cond a or cond b or cond c or cond d cond a = Ar-X, DMSO, Et₃N, 120 °C cond b = Ar-X, Cs₂CO₃, XantPhos, Pd₂(dba)₃, dioxane, DMSO cond c = Ar-X, 1,4-dioxane, Et₃N, 100 °C cond d = Ar-X, NaOtBu, catalyst 1, Pd₂(dba)₃, dioxane, DMSO

Example 1 :F,

1,5-anhydro-2,3-dideoxy-3-{[(1 R,3S)-3-ethyl-3-({( 1 S,4S)-5-[4(trifluoromethyl)pyridin-2-yl]-2,5-diazabicyclo[2.2.1]hept-2yl}carbonyl)cyclopentyl]amino}-4-O-methyl-D-erythro-pentitol

Step 1: Préparation of methyl (1R.4S)-4-(2,5-dimethvl-1H-pyrrol-1-vl)-1ethylcvclopent-2-ene-1-carboxylate

A -40°C solution of 2M (in THF/ethylbenzene/heptane) LDA (68ml, 138mmol) in tetrahydrofuran (120ml) was treated with methyl (1 R,4S)-4-(2,5-dimethyl-1Hpyrrol-1-yl)cyclopent-2-ene-1-carboxylate (14.02g, 63.9mmol) while keeping the température less than -33°C. The cold reaction was stirred for 40 minutes and then a solution of ethyl iodide (13.63g, 87.4mmol) in tetrahydrofuran (5ml) was added slowly while keeping the température less than -33°C. The reaction was stirred with the cold bath in place for four hours and allowed to slowly warm. The reaction was poured înto NH4CI soin (300ml), then extracted with ethyl acetate (2x200ml), washed with brîne dried over MgSO₄, and concentrated under reduced pressure. The resulting brown oil was passed through acolumn of silica gel with10% ethyl acetate/hexanes to give methyl (1 R,4S )-4-(2,5-dimethyl-1 Hpyrrol-1-yl)-1-ethylcyclopent-2-ene-1-carboxylate as a brown oil (12.96g, 82%). ¹H NMR (400 MHz, CDCI₃) d ppm 5.98-5.96 (1H), 5.93-5.91 (1H), 5.73 (2H), 5.30-5.24 (1H), 3.71 (3H), 2.45-2.34 (2H), 2.19 (6H), 1.79-1.73 (2H), 0.91-0.87 (3H).

Step 2: Préparation of(1 R,4S )-4-(2,5-dimethvl-1H-pvrrol-1-vl)-1-ethvlcvclopent-2 ene-1-carboxylic acid

A solution of methyl (1R,4S)-4-(2,5-dimethyl-1H-pyrrol-1-yl)-1-ethylcyclopent-2ene-1-carboxylate (12.94g, 52.3mmol) in methanol (100ml) was treated with 2.5N NaOH (30ml, 75.0mmol) and stirred at room température. After 15 hours more 2.5N NaOH (10ml, 25.0mmol) was added and the reaction was stirred for an additional four days. The methanol was removed under reduced pressure and the residue partitioned between diethyl ether and water. The layers were separated and the aqueous was acidified with 4N HCl, extracted twice with ethyl acetate, washed with brine, dried over MgSO₄, and concentrated under reduced pressure to give (1 R,4S)-4-(2,5-dimethyl-1H-pyrrol-1-yl)-1-ethylcyclopent-2-ene1-carboxylic acid as a brown oil (11.83g, 97%). ¹H NMR (400 MHz, CDCI₃) d ppm 5.98-5.96 (2H), 5.73 (2H), 5.30-5.26 (1H), 2.47-2.35 (2H), 2.20 (6H), 1.821.77 (2H), 0.96-0.92 (3H).

Step 3: Préparation of tert-butyl (1S.4S)-5-ffî1R,4S)-4-(2,5-dimethvl-1H-pvrrol-1vl)-1-ethvlcvclopent-2-en-1-vl1carbonvn-2.5-diazabicvclo(2.2.1lheptane-2carboxylate

BOP= benzotriazol-l-yloxytris(dimethylamino) phosphoniumhexafluorophosphate

A solution of (1 R,4S )-4-(2,5-dimethyl-1H-pyrrol-1-yl)-1-ethylcyclopent-2-ene-1carboxylic acid (6.59g, 28.2mmol) in DMF (40ml) was treated with N,Ndüsopropylethylamine (14.7ml, 84.4mmol) and BOP (14.51g, 32.8mmol) and stirred under nitrogen at room température for 20 minutes. A solution of (1S,4S)2-BOC-2,5-diazabicyclo[2.2.1]heptane (5.66g,28.5mmol) in DMF (5ml) was added and the reaction was stirred for 24 hours. The reaction was diluted with ethyl acetate (100ml) and washed with 60% saturated NaHCO₃ (125ml). The aqueous layer was extracted with ethyl acetate. The organic layers were combined, washed with brine, dried over MgSO₄ and concentrated under reduced pressure to give a brown oil (21.25g). The oil was passed through a column of silica gel with 50% ethyl acetate/hexanes to give tert-butyl (1S,4S)-5{[(1R,4S)-4-(2,5-dimethyl-1H-pyrrol-1-yl)-1-ethylcyclopent-2-en-1-yl]carbonyl}2,5-diazabicycio[2.2.1]heptane-2-carboxylate as a white foam (8.14g, 70%). ¹H NMR (400 MHz, CDCI₃) d ppm 6.24-6.17 (1H), 5.90-5.86 (1H), 5.73-5.68 (2H), 5.34-5.22 (1H), 5.02-4.41 (2H), 3.71-3.24 (4H), 2.61-2.39 (1H), 2.27-2.16 (7H), 1.89-1.59 (4H), 1.49-1.32 (9H), 0.95-0.85 (3H).

Step 4: Préparation of tert-butvl (1S,4S)-5-(f(1R,4S)-4-amino-1-ethvlcvclopent-2en-1-vl1carbonvl}-2,5-diazabicvclof2.2.11heptane-2-carboxvlate

A solution of tert-butyl (1S,4S)-5-{[(1R,4S)-4-(2,5-dimethyl-1H-pyrrol-1-yl)-1ethylcyclopent-2-en-1-yl]carbonyl}-2,5-diazabicyclo[2.2.1]heptane-2-carboxylate (2.61g, 6.3mmol) in methanol (40ml) and water (10ml) was treated with hydroxylamine hydrochloride (2.73g, 39.1 mmol) and 50 weight% solution of hydroxylamine (2.4ml, 39.2mmol). The reaction was stirred under nitrogen and heated to 68°C for 38.5 hours. The reaction was cooled to room température, diluted with water, and made basic (pH—11 ) by the addition of 2.5N NaOH. The reaction mixture was extracted with ethyl acetate (3x100ml), washed with brine, dried over MgSÛ4 and concentrated under reduced pressure to give a crude mixture of tert-butyl (1S,4S)-5-{[(1R,4S)-4-amino-1-ethylcyclopent-2-en-1yl]carbonyl}-2,5-diazabicyclo[2.2.1]heptane-2-carboxylate as a yellow oil (2.68g, theoretical yield 2.12g) which was used without further purification in the next step.

Step 5: Préparation of tert-butyl (1S,4S)-5-{r(1S,3R)-3-amino-1ethvlcvclopentvl1carbonvl)-2,5-diazabicvclof2.2.11heptane-2-carboxylate

A mixture of tert-butyl (1S,4S)-5-{[(1R,4S)-4-amino-1-ethylcyclopent-2-en-1yl]carbonyl}-2,5-diazabicyclo[2.2.1]heptane-2-carboxylate (2.12g, 6.32mmol) and 5% palladium on carbon in methanol (35ml) was stirred at room température under 48psi of hydrogen for 21 hours. The reaction was filtered through celite and the filter cake washed with methanol. The filtrate and washings were concentrated under reduced pressure to give tert-butyl (1S,4S)-5-{[( 1 S,3R)-3amino-1-ethylcyclopentyl]carbonyl}-2,5-diazabicyclo[2.2.1]heptane-2-carboxylate as a brown oil/foam (2.46g, theoretical yield 2.13g) which was used without further purification in the next step.

Step 6: Préparation of (1S,4S)-tert-butvl 5-((1S,3R)-1-ethyl-3-((3S,4R)-3methoxv-tetrahydro-2H-pyran-4-vlamino)cvclopentanecarbonvl)-2,5-diazabicvclof2.2.1 lheptane-2-carboxylate

Γκ

A 0°C solution of tert-butyl (1S,4S)-5-{[(1S,3R)-3-amino-1ethylcyclopentyl]carbonyl}-2,5-diazabicyclo[2.2.1]heptane-2-carboxylate (1.58g, 4.7mmol) in dichloromethane (25ml) was treated with sodium triacetoxyborohydride (2.36g, 11.1mmol) and 3R)-3-methoxytetrahydro-4Hpyran-4-one (0.76g, 7.6mmol). The reaction was stirred under nitrogen at 0°C for 30 minutes then allowed to warm to room température and stirred for 15 hours. The reaction was treated with 2.5N NaOH (10mL) and stirred for 10 minutes. The reaction was diluted with water and the layers separated. The aqueous layer was extracted twice with ethyl acetate. The dichloromethane layer was concentrated under reduced pressure and partitioned between ethyl acetate and water. The organic layers were combined, washed with brine, dried over MgSO4, concentrated under reduced pressure and purified with the Biotage (0-100% methanol/ethyl acetate, 15 column volumes) to give the desired product, 1,5anhydro-3-{[(1 R,3S)-3-{[(1S,4S)-5-(tert-butoxycarbonyl)-2,5diazabicyclo[2.2.1]hept-2-yl]carbonyl}-3-ethylcyclopentyl]amino}-2,3-dideoxy-4-Omethyl-D-erythro-pentitol. . LC/MS (M+H) = 452.3124 exp, 452.3135 obs; ¹H NMR (400 MHz, CDCI₃) d ppm 4.94-4.41 (2H), 4.11-4.04 (1 H), 3.97-3.91 (1 H), 3.53-3.18 (11 H), 2.83-2.75 (1 H), 2.60-2.30 (1 H), 2.03-1.55 (10H), 1.48-1.37 (11 H), 0.85-0.78 (3H)

Step 7: Préparation of (1S,4S)-2,5-diaza-bicvclor2.2.11heptan-2-vl((1S,3R)-1ethvl-3-((3S.4R)-3-methoxv-tetrahvdro-2H-pyran-4vlamino)cvclopentvl)methanone

To a solution of (1S,4S)-tert-butyl 5-((1S,3R)-1-ethyl-3-((3S,4R)-3-methoxytetrahydro-2H-pyran-4-ylamino)cyclopentanecarbonyl)-2,5-diazabicyclo[2.2.1]heptane-2-carboxylate( 1.22g, 2.89mmol) in 1,4-dioxane (15ml) was added 4N HCI/1,4-dioxane (15ml). The reaction was stirred at room température for 18 hours. The liquid was decanted leaving a gummy solid which was dissolved in methanol and concentrated under reduced pressure. The residue was dissolved in methylene chloride and concentrated to give the product HCl sait of as a brown foam (1.23g, theoretical yield 1.03g) which was used in the next step without further purification.

Step 8: Préparation of 1,5-anhvdro-2.3-dideoxv-3-i[(1R,3S)-3-ethvl-3-(f(1S,4S)-5|4-(trifluoromethvl)pvridin-2-vl1-2,5-diazabicvclof2.2.11hept-2 vRcarbonvl)cvclopentvllaminoM-O-methvl-D-ervthro-pentitol

To a solution of HCl sait of (1S,4S)-2,5-diaza-bicyclo[2.2.1]heptan-2-yl((1S,3R)-1ethyl-3-((3S,4R)-3-methoxy-tetrahydro-2H-pyran-4ylamino)cyclopentyl)methanone (118mg, 0.33mmol) in DMSO (2ml) was added triethylamine (0.15ml, 1.08mmol) and the 2-chloro-4-(trifluoromethyl)pyridine (183mg, 1.01mmol). The reaction was heated to 120°C for 15 hours. The reaction was cooled to room température, and added to stirring ice water. The mixture was extracted with ethyl acetate(3x). The combined organics were washed with brine, dried over MgSO4 concentrated under reduced pressure and purified with the Biotage (0-100% methanol/ethyl acetate, 15 column volumes) to give the product as a brown foam (40mg 26%). LC/MS (M+H) = 497.2739 exp, 497.2884 obs; ¹H NMR (400 MHz, CDCI₃) d ppm 8.25-8.24 (1H), 6.75-6.74 (1H), 6.51-6.45 (1H), 5.13-4.76 (2H), 4.10-4.03 (1H), 3.95-3.90 (1H), 3.72-3.16 (11 H), 2.82-2.67 (1H), 2.58-2.36 (1H), 2.06-1.36 (12H), 0.86-0.67 (3H)

Example 2

1,5-anhydro-2,3-dideoxy-3-{[(1 R,3S)-3-ethyl-3-({(1 S,4S)-5-[4(trifluoromethyl)pyrimidin-2-yl]-2,5-diazabicyclo[2.2.1]hept-2yl}carbonyl)cyclopentyl]amino}-4-O-methyl-D-erythro-pentitol

Prepared as described in example 1 using 2-chloro-4-(trifluoromethyl)pyrimidine in place of 2-chloro-4-(trifluoromethyl)pyridine and modifying step 8 as follows. The HCl sait of amine (91 mg, 0.25mmol) was placed in flask with 1,4-dioxane (3ml). Triethylamine (0.12ml, 0.86mmol) was added as well as 2-chloro-4(trifluoromethyl)pyrimidine (148mg, 0.81 mmol). DMSO (0.3ml) was added for solubility and the reaction was heated to 100°C for 20 hours. The reaction was cooled to room température and concentrated under reduced pressure. The residue was partitioned between ethyl acetate and water. The aqueous layer was extracted with ethyl acetate and the combined organics were washed with brine, dried over MgSO₄, concentrated under reduced pressure and purified with the Biotage (0-100% methanol/ethyl acetate 15 column volumes) to give the product as a brown foam (43mg, 58%). LC/MS (M+H) = 498.2692 exp, 498.2799 obs; ¹H NMR (400 MHz, CDCI₃) d ppm 8.48-8.47 (1 H), 6.80-6.78 (1H), 5.16-4.71 (2H),

6l

4.09-4.00 (1H), 3.93-3.86 (1H), 3.70-3.51 (3H), 3.45-3.13 (8H), 2.85-2.66 (1 H). 2.60-2.33 (1H), 2.06-1.35 ( 12H). 0.87-0.66 (3H)

Example 3

N^N

1,5-anhydro-2,3-dideoxy-3-{[(1 R,3S)-3-ethyl-3-({(1S,4S)-5-[6(trifluoromethyl)pyrimidin-4-yl]-2,5-diazabicyclo[2.2.1]hept-2yl}carbonyl)cyclopentyl]amino}-4-O-methyl-D-erythro-pentitol

Prepared as described in example 1 using 4-chloro-6-(trifluoromethyl)pyrimidine in place of 2-chloro-4-(trifluoromethyl)pyridine and modifying step 8 as follows.The HCl sait of amine (227mg, 0.63mmol) was placed in a flask with 1,4dioxane (3ml). To the stirred solution was added Xantphos (26mg, 0.045mmol), Pd₂(dba)₃ (39mg, 0.042mmol), Cs₂CO₃ (258mg, 0.79mmol), and 4-chloro-6(trifluoromethyl)pyrimidine (261 mg, 1.43mmol) dissolved in 1,4-dioxane (1ml). DMSO (0.3ml) was added for solubility and the reaction was heated to 100°C for 15 hours. The reaction was allowed to cool to room température and filtered through celite. The filtrate was concentrated under reduced pressure and purified with the Biotage (0-100% methanol/ethyl acetate 15 column volumes) to give the product as a brown foam (93mg, 37%). LC/MS (M+H) = 498.2692 exp, 498.2853 obs; ¹H NMR (400 MHz, CDCI₃) d ppm 8.63 (1 H), 6.76-6.42 (1 H), 5.31-4.54 (2H), 4.12-4.03 (1H), 3.96-3.85 (1 H), 3.72-3.15 (11 H), 2.80-2.66 (1H), 2.57-2.35 (1H), 2.09-1.33 (12H), 0.88-0.64 (3H)

Example 4

1,5-anhydro-2,3-dideoxy-3-{[(1R,3S)-3-ethyl-3-({(1S,4S)-5-[6(trifluoromethyl)pyrazin-2-yl]-2,5-diazabicyclo[2.2.1]hept-2yl}carbonyl)cyclopentyl]amino)-4-O-methyl-D-erythro-pentitol

Prepared as described in example 3 using 2-iodo-6-(trifluoromethyl)pyrazine in place of 4-chloro-6-(trifluoromethyl)pyrimidine in step 8. LC/MS (M+H) = 498.2692 exp, 498.2867 obs; ¹H NMR (400 MHz, CDCI₃) d ppm 8.16 (1H), 8.057.97 (1 H), 5.18-4.78 (2H), 4.12-4.02 (1H), 3.99-3.88 (1 H), 3.74-3.14 (11 H), 2.822.66 (1H), 2.61-2.36 (1H), 2.07-1.36 (12H), 0.86-0.68 (3H)

Example 5

1,5-anhydro-2,3-dideoxy-3-{[(1 R,3S)-3-ethyl-3-({(1S,4S)-5-[2(trifluoromethyl)pyrimidin-4-yl]-2,5-diazabicyclo[2.2.1]hept-2yl}carbonyl)cyclopentyl]amino}-4-O-methyl-D-erythro-pentitol

Prepared as described in example 3 using 4-chloro-2-(trifluoromethyl)pyrimidine in place of 4-chloro-6-(trifluoromethyl)pyrimidine in step 8. LC/MS (M+H) = 498.2692 exp, 498.2839 obs; ¹H NMR (400 MHz, CDCI₃) d ppm 8.24-8.22 (1H), 6.54-6.19 (1 H), 5.31-4.50 (2H), 4.09-4.00 (1H), 3.90-3.85 (1H), 3.72-3.17 (11 H), 2.78-2.62 (1H), 2.55-2.35 (1H), 2.04-1.32 (12H), 0.85-0.63 (3H)

Example 6

1,5-anhydro-2,3-dideoxy-3-{[(1 R,3S)-3-ethyl-3-({(1 S,4S)-5-[6(trifluoromethyl)pyridin-2-yl]-2,5-diazabicyclo[2.2.1]hept-2yl}carbonyl)cyclopentyl]amino}-4-O-methyl-D-erythro-pentitol

Prepared as described in example 1 using 2-chloro-6-(trifluoromethyl)pyridine in place of 4-chloro-6-(trifluoromethyl)pyrimidine in step 8. LC/MS (M+H) = 497.2739 exp, 497.2641 obs; ¹H NMR (400 MHz, CDCI₃) d ppm 7.56-7.52 (1H), 6.92-6.90 (1 H), 6.52-6.41 (1H), 5.11-4.73 (2H), 4.08-4.03 (1H), 3.95-3.89 (1H), 3.73-3.13 (11 H), 2.82-2.64 (1H), 2.58-2.35 (1H), 2.05-1.35 (12H), 0.85-0.66 (3H)

Example 7

1,5-anhydro-2,3-dideoxy-3-{[(1 R,3S)-3-ethyl-3-({(1 S,4S)-5-[2(trifluoromethyl)pyridin-4-yl]-2,5-diazabicyclo[2.2,1]hept-2yl}carbonyl)cyclopentyl]amino}-4-O-methyl-D-erythro-pentitol

Prepared as described in example 3 using using 4-iodo-2(trifluoromethyl)pyridine in place of 4-chloro-6-(trifluoromethyl)pyrimidine in step

8. LC/MS (M+H) = 497.2739 exp, 497.2878 obs; ¹H NMR (400 MHz, CDCI₃) d ppm 8.30-8.28 (1H), 6.76-6.69 (1H), 6.52-6.44 (1H), 5.16-4.79 (1H), 4.54 (1H), 4.07-4.04 (1 H), 3.93-3.90 (1 H), 3.68-3.14 (11 H), 2.81-2.64 (1 H), 2.59-2.31 (1 H), 2.08-1.35 (12H), 0.87-0.65 (3H)

Example 8

1,5-anhydro-2,3-dideoxy-3-{[(1R,3S)-3-ethyl-3-({(1S,4S)-5-[3-fluoro-4(trifluoromethyl)pyridin-2-yl]-2,5-diazabicyclo[2.2.1]hept-2yl}carbonyl)cyclopentyl]amino}-4-O-methyl-D-erythro-pentitol

Prepared as described in example 3 using 2-chloro-3-fluoro-4(trifluoromethyl)pyridine in place of 4-chloro-6-(trifluoromethyl)pyrimidine in step 8. LC/MS (M+H) = 515.2645 exp, 515.2772 obs; ¹H NMR (400 MHz, CDCI3) d ppm 8.00-7.99 (1H), 6.75-6.73 (1H), 5.09-4.69 (2H), 4.09-4.04 (1H), 3.96-3.90 (1H), 3.86-3.78 (1H), 3.69-3.53 (3H), 3.42-3.17 (7H), 2.81-2.68 (1H), 2.57-2.38 (1H), 2.04-1.36 (12H), 0.88-0.69 (3H)

Example 9

1,5-anhydro-2,3-dideoxy-3-{[(1R,3S)-3-methyl·3-({(1S,4S)-5-[4(trifluoromethyl)pyridin-2-yl]-2,5-diazabicyclo[2.2.1]hept-2yl}carbonyl)cyclopentyl]amino)-4-O-methyl-D-erythro-pentitol

Prepared as described in example 1 using methyl iodide in place of ethyl iodide in step 1. LC/MS (M+H) = 483.2583 exp, 483.2543 obs; ¹H NMR (400 MHz, CDCI₃) d ppm 8.25-8.24 (1H), 6.75-6.74 (1H), 6.51-6.45 (1H), 5.09-4.76 (2H), 4.09-4.03 (1 H), 3.96-3.90 (1 H), 3.70-3.23 (11 H), 2.81-2.65 (1 H), 2.53-2.27 (1 H), 2.07-1.02 (13H); HRMS m/z 483.2543 (calcd for M+H, 483.2583).

Example 10

TT

1,5-anhydro-2,3-dideoxy-3-{[(1R,3S)-3-methyl-3-({(1S,4S)-5-[6(trifluoromethyl)pyrazin-2-yl]-2,5-diazabicyclo[2.2.1]hept-2yl}carbonyl)cyclopentyl]amino}-4-O-methyl-D-erythro-pentitol

Prepared as described in example 3 using methyl iodide in place of ethyl iodide in step 1 and 2-iodo-6-(trifluoromethyl)pyrazine in place of 4-chloro-6(trifluoromethyl)pyrimidine in step 8. LC/MS (M+H) = 483.2583 exp, 483.2543 obs; ¹H NMR (400 MHz, CDCI₃) d ppm 8.25-8.24 (1 H), 6.75-6.74 (1 H), 6.51-6.45 (1H), 5.09-4.76 (2H), 4.09-4.03 (1H), 3.96-3.90 (1H), 3.70-3.23 (11 H), 2.81-2.65 (1H), 2.53-2.27 (1H), 2.07-1.02 (13H)

Example 11

1,5-anhydro-2,3-dideoxy-3-{[(1R,3S)-3-isopropyl-3-({(1S,4S)-5-[4(trifluoromethyl)pyridin-2-yl]-2,5-diazabicyclo[2.2.1]hept-2yl}carbonyl)cyclopentyl]amino}-4-O-methyl-D-erythro-pentitol

Prepared as described in example 1 starting with (1S,4S)-4-(2,5-dimethyl-1Hpyrrol-1-yl)-1-isopropylcyclopent-2-ene-1-carboxylic acid and skipping steps 1 and 2. LC/MS (M+H) = 511.2896 exp, 511.3218 obs; ¹H NMR (400 MHz, CDCI₃) d ppm 8.23-8.22 (1 H), 6.73-6.72 (1 H), 6.49-6.44 (1 H), 5.11-4.73 (2H), 4.064.03 (1H), 3.92-3.89 (1H), 3.67-3.52 (2H), 3.44-3.22 (8H), 3.12-3.03 (1H), 2.802.61 (1H), 2.51-2.40 (1H), 2.09-1.72 (6H), 1.68-1.42 (4H), 1.35-1.25 (1H), 0.920.75 (6H)

Example 12

1,5-anhydro-2_t3-dideoxy-3-{[(1R,3S)-3-isopropyl-3-({(1S,4S)-5-[4(trifluoromethyl)pyrimidin-2-yl]-2,5-diazabicyclo[2.2.1]hept-2yl}carbonyl)cyclopentyl]amino}-4-O-methyl-D-erythro-pentitol

Préparée! as described in example 2 starting with (1 S,4S)-4-(2,5-dimethyl-1 Hpyrrol-1-yl)-1-isopropylcyclopent-2-ene-1-carboxylic acid and skipping steps 1 and 2. LC/MS (M+H) = 512.2849 exp, 512.2827 obs; ¹H NMR (400 MHz, CDCI₃)d ppm 8.45-8.44(1 H), 6.77-6.76 (1H), 5.09-4.71 (2H), 4.07-4.01 (1H), 3.923.87 (1H), 3.67-3.51 (3H), 3.42-3.23 (7H), 3.15-3.08 (1H), 2.83-2.76 (1H), 2.582.33 (2H), 2.11-1.56 (8H), 1.51-1.42 (1H), 1.38-1.30 (1H), 0.91-0.74 (6H)

Example 13 :F.

1,5-anhydro-2,3-dideoxy-3-{[(1R,3S)-3-isopropyl-3-({(1S,4S)-5-[6(trifluoromethyl)pyrimidin-4-yl]-2,5-diazabicyclo[2.2.1]hept-2yl}carbonyl)cyclopentyl]amino}-4-O-methyl-D-erythro-pentitol

Prepared as described in example 3 starting with (1S,4S)-4-(2,5-dimethyl-1Hpyrrol-1-yl)-1-isopropylcyclopent-2-ene-1-carboxylic acid and skipping steps 1 and 2. LC/MS (M+H) = 512.2849 exp, 512.3227 obs; ¹H NMR (400 MHz, CDCI₃) d ppm 8.61 (1 H), 6.73-6.44 (1 H), 5.25-4.52 (2H), 4.05-4.01 (1 H), 3.91-3.86 (1H), 3.68-3.46 (2H), 3.38-3.17 (8H), 3.10-3.03 (1H), 2.76-2.63 (1H), 2.48-2.37 (1H), 2.07-1.39 (10H), 1.34-1.23 (1H), 0.90-0.74 (6H)

Example 14

1,5-an hyd ro-2,3-d i deoxy-3-{[( 1R, 3S )-3-i so pro pyl-3-( {( 1S, 4S )-5-[6 (trifluoromethyl)pyrazin-2-yl]-2,5-diazabicyclo[2.2.1]hept-2yl}carbonyl)cyclopentyl]amino}-4-O-methyl-D-erythro-pentitol

Prepared as described in example 3 starting with (1S,4S)-4-(2,5-dimethyl-1Hpyrrol-1-yl)-1-isopropylcyclopent-2-ene-1-carboxylic acid and skipping steps 1 and 2 and using 2-iodo-6-(trifluoromethyl)pyrazine in place of 4-chloro-6(trifluoromethyl)pyrimidine in step 8. LC/MS (M+H) = 512.2849 exp, 512.3221 obs; ¹H NMR (400 MHz, CDCI₃) d ppm 8.12 (1 H). 8.01-7.97 (1H), 5.14-4.75 (2H), 4.05-4.01 (1H), 3.90-3.87 (1H), 3.67-3.56 (2H), 3.44-3.20 (8H), 3.11-3.02 (1H), 2.78-2.60 (1H), 2.48-2.39 (1H), 2.10-1.86 (4H), 1.82-1.71 (2H), 1.68-1.53 (2H), 1.50-1.38 (2H), 1.33-1.24 (1H), 0.91-0.74 (6H)

Example 15

1,5-anhydro-2,3-dideoxy-3-{[(1R,3S)-3-isopropyl-3-({(1S,4S)-5-[2(trifluoromethyl)pyrimidin-4-yl]-2,5-diazabicyclo[2.2.1]hept-2yl}carbonyl)cyclopentyl]amino}-4-O-methyl-D-erythro-pentitol

Prepared as described in example 3 starting with (1S,4S)-4-(2,5-dimethyl-1Hpyrrol-1-yl)-1-isopropylcyclopent-2-ene-1-carboxylic acid and skipping steps 1 and 2 and using 4-chloro-2-(trifluoromethyl)pyrimidine in place of 4-chloro-6(trifluoromethyl)pyrimidine in step 8, LC/MS (M+H) = 512.2849 exp, 512.2891 obs; ¹H NMR (400 MHz, CDCI₃) d ppm 8.25-8.24 (1 H), 6.52-6.23 (1 H), 5.30-4.47 (2H), 4.05-4.02 (1H), 3.91-3.88 (1 H), 3.70-3.54 (2H), 3.52-3.18 (8H), 3.12-3.03 (1H), 2.78-2.61 (1H), 2.49-2.39 (1 H), 2.08-1.85 (5H), 1.82-1.72 (1 H), 1.68-1.41 (4H), 1.35-1.24 (1 H), 0.93-0.75 (6H)

Example 16

1,5- anhydro-2,3-dideoxy-3-{[(1 R,3S)-3-isopropyl-3-({(1 S,4S)-5-[6(trifluoromethyl)pyridin-2-yl]-2,5-diazabicyclo[2.2.1]hept-2yl}carbonyl)cyclopentyl]amino}-4-O-methyl-D-erythro-pentitol

Prepared as described in example 1 starting with (1S,4S)-4-(2,5-dimethyl-1Hpyrrol-1-yl)-1-isopropylcyclopent-2-ene-1-carboxylic acid and skipping steps 1 and 2 and using 2-chloro-6-(trifluoromethyl)pyridine in place of 2-chloro-4(trifluoromethyl)pyridine in step 8. LC/MS (M+H) = 511.2896 exp, 511.2856 obs;

¹H NMR (400 MHz, CDCI₃) d ppm 7.54-7.50 (1H), 6.90-6.88 (1 H), 6.50-6.41 (1H), 5.08-4.70 (2H), 4.08-3.99 (1H), 3.94-3.87 (1H), 3.73-3.46 (2H), 3.46-3.13 (8H), 3.13-2.97 (1H), 2.81-2.58 (1H), 2.52-2.38 (1H), 2.10-1.70 (5H), 1.70-1.40 (5H), 1.36-1.25(1 H), 0.94-0.73 (6H)

0.75 (6H)

Example 17

1,5-anhydro-2,3-dideoxy-3-{[(1R,3S)-3-isopropyl-3-({(1S,4S)-5-[2(trifluoromethyl)pyridin-4-yl]-2,5-diazabicyclo[2.2.1]hept-2yl}carbonyl)cyclopentyl]amino}-4-O-methyl-D-erythro-pentitol

Prepared as described in example 3 starting with (1S,4S)-4-(2,5-dimethyl-1H10 pyrrol-1-yl)-1-isopropylcyclopent-2-ene-1-carboxylic acid and skipping steps 1 and 2 and using 4-iodo-2-(trifluoromethyl)pyridine in place of 2-chloro-4(trifluoromethyl)pyridine in step 8. LC/MS (M+H) = 511.2896 exp, 511.2958 obs;

¹H NMR (400 MHz, CDCI₃) d ppm 8.27-8.26 (1 H), 6.72-6.69 (1 H), 6.49-6.44 (1 H), 5.09-4.75 (1H), 4.52 (1H), 4.05-4.02 (1H), 3.91-3.88 (1H), 3.65-3.50 (3H), 3.4115 3.03 (8H), 2.78-2.55 (1H), 2.49-2.35 (1H), 2.08-1.87 (4H), 1.84-1.72 (2H), 1.681.53 (2H), 1.51-1.38 (2H), 1.34-1.26 (1 H), 0.92-0.75 (6H)

Example 18

1,5-anhydro-2,3-dideoxy-3-{[(1R,3S)-3-isopropyl-3-({(1S,4S)-5-[5(trifluoromethyl)pyridazin-3-yl]-2,5-diazabicyclo[2.2.1]hept-2yl}carbonyl)cyclopentyl]amino}-4-O-methyl-D-erythro-pentitol

Prepared as described in example 2 starting with (1 S,4S)-4-(2,5-dimethyl-1Hpyrrol-1-yl)-1-isopropylcyclopent-2-ene-1-carboxylic acid and skipping steps 1 and 2 and using 3-chloro-5-(trifluoromethyl)pyridazine in place of 2-chloro-4(trifluoromethyl)pyrimidine in step 8. LC/MS (M+H) = 512.2849 exp, 512,2864 obs; ¹H NMR (400 MHz, CDCI₃) d ppm 8.73 (1H), 6.75-6.68 (1 H), 5.32-4.79 (2H), 4.07-4.04 (1H), 3.93-3.89 (1H), 3.80-3.55 (2H), 3.52-3.16 (8H), 3.13-3.03 (1H), 2.82-2.63 (1 H), 2.51-2.38 (1H), 2.09-1.42 (10H), 1.36-1.24 (1H), 0.93-0.75 (6H)

Example 19

1,5-anhydro-3-{[(1R,3S)-3-isopropyl-3-({(1S,4S)-5-[2,6-bis(trifluoromethyl)pyridin4-yl]-2,5-diazabicyclo[2.2.1]hept-2-yl}carbonyl)-3-isopropylcyclopentyl]amino}2,3-dideoxy-4-O-methyl-D-erythro-pentitol

Prepared as described in example 3 starting with (1S,4S)-4-(2,5-dimethyl-1Hpyrrol-1-yl)-1-isopropylcyclopent-2-ene-1-carboxylic acid and skipping steps 1 and 2 and using 4-chloro-2,6-bis(trifluoromethyl)pyridine in place of 4-chloro-6(trifluoromethyl)pyrimidine in step 8. LC/MS (M+H) = 579.2770 exp, 579.2667 obs; ¹H NMR (400 MHz, CDCI₃) d ppm 6.94-6.67 (2H), 5.17-4.78 (1H), 4.60 (1H), 4.06-4.04 (1H), 3.92-3.89 (1H), 3.68-3.57 (2H), 3.42-3.16 (8H), 3.13-3.05 (1H), 2.79-2.62 (1H), 2.48-2.37 (1H), 2.08-1.86 (3H), 1.83-1.54 (6H), 1.52-1.42 (1H), 1.37-1.24 (1H), 0.92-0.76 (6H)

Example 20

1,5-anhydro-2,3-dideoxy-3-{[(1 R,3S)-3-({(1S,4S)-5-[3-fluoro-4(trifluoromethyl)pyridin-2-yl]-2,5-diazabicyclo[2.2.1]hept-2-yl}carbonyl)-3isopropylcyclopentyl]amino}-4-O-methyl-D-erythro-pentitol

Prepared as described in example 3 starting with (1S,4S)-4-(2,5-dimethyl-1Hpyrrol-1-yl)-1-isopropylcyclopent-2-ene-1-carboxylic acid and skipping steps 1 and 2 and using 2-chloro-3-fluoro-4-(trifluoromethyl)pyridine in place of 4-chloro6-(trifluoromethyl)pynmidine in step 8. LC/MS (M+H) - 529.2802 exp, 529.3431 obs; ¹H NMR (400 MHz, CDCI₃) d ppm 7.98-7.97 (1H), 6.73-6.71 (1H), 5.07-4.67 (2H), 4.08-4.02 (1H), 3.94-3.88 (1H), 3.84-3.78 (1H), 3.64-3.47 (3H), 3.40-3.21 (6H), 3.13-3.04 (1H), 2.80-2.69 (1H), 2.49-2.39 (1H), 2.10-1.56 (9H), 1.54-1.42 (1 H), 1.37-1.28 (1 H), 0.92-0.75 (6H)

Example 21

1,5- anhydro-2,3-dideoxy-3-{[( 1 R,3S )-3-isopropyl-3-({( 1 S,4S )-5-[6-methyl-4(trifluoromethyl)pyridin-2-yl]-2,5-diazabicyclo[2.2.1]hept-2- yl}carbonyl)cyclopentyl]amino}-4-O-methyl-D-erythro-pentitol

Prepared as described in example 3 starting with (1S,4S)-4-(2,5-dimethyl-1Hpyrrol-1-yl)-1-isopropylcyclopent-2-ene-1-carboxylic acid and skipping steps 1 and 2 and using 2-chloro-6-methyl-4-(trifluoromethyl)pyridine in place of 4-chloro6-(trifluoromethyl)pyrimidine in step 8. LC/MS (M+H) = 525.3052 exp, 525.3242 obs; ¹H NMR (400 MHz, CDCI₃) δ ppm 6.61 (1H), 6.29-6.24 (1H), 5.14-4.71 (2H), 4.17-4.04 (2H), 3.98-3.90 (1H), 3.70-3.49 (3H), 3.48-3.13 (7H), 2.91-2.72 (1H), 2.50-2.32 (4H), 2.11-1.39 (11 H), 0.94-0.71 (6H)

Example 22

1,5-anhydro-2,3-dideoxy-3-{[(1R,3S)-3-isopropyl-3-({(1S,4S)-5-[3(trifluoromethyl)phenyl]-2,5-diazabicyclo[2.2.1]hept-2yl}carbonyl)cyclopentyl]amino}-4-O-methyl-D-erythro-pentitol

Prepared as described in example 3 starting with (1S,4S)-4-(2,5-dimethyl-1Hpyrrol-1-yl)-1-isopropylcyclopent-2-ene-1-carboxylic acid and skipping steps 1 and 2 and using 3-iodo-benzotrifluoride in place of 4-chloro-6(trifluoromethyl)pyrimidine in step 8. LC/MS (M+H) = 510.2943 exp, 510.2976 obs; ¹H NMR (400 MHz, CDCI₃) d ppm 7.30-7.25 (1H), 6.92-6.90 (1H), 6.72-6.65 (2H), 5.06-4.68 (1 H), 4.44 (1 H), 4.07-3.99 (1 H), 3.94-3.86 (1 H), 3.71-3.51 (2H), 3.48-3.19 (7H), 3.14-3.01 (1H), 2.81-2.69 (1H), 2.61-2.35 (1H), 2.09-1.54 (9H), 1.51-1.40 (1H), 1.35-1.27 (1H), 0.94-0.72 (6H)

Example 23

1,5-anhydro-2,3-dideoxy-3-{[(1 R,3S)-3-({(1S,4S)-5-[3-fluoro-5(trifluoromethyl)phenyl]-2,5-diazabicyclo[2.2.1]hept-2-yl}carbonyl)-3isopropylcyclopentyl]amino}-4-O-methyl-D-erythro-pentitol

Prepared as described in example 3 starting with (1S,4S)-4-(2,5-dimethyl-1Hpyrrol-1-yl)-1-isopropylcyclopent-2-ene-1-carboxylic acid and skipping steps 1 and 2 and using 3-bromo-5-fluorobenzotrifluoride in place of 4-chloro-6(trifluoromethyl)pyrimidine in step 8. LC/MS (M+H) = 528.2849 exp, 528.2996 15 obs; ¹H NMR (400 MHz, CDCI₃) d ppm 6.61-6.58 (1H), 6.48 (1H), 6.35-6.33 (1 H), 5.06-4.69 (1H), 4.39 (1H), 4.06-4.00 (1H), 3.94-3.86 (1H), 3.65-3.50 (2H), 3.46-3.15 (7H), 3.12-3.00 (1H), 2.80-2.70 (1H), 2.49-2.32 (1H), 2.06-1.73 (6H), 1.69-1.40 (4H), 1.36-1.27 (1H), 0.94-0.72 (6H)

Scheme 2. Préparation of examples 24-44

a) LDA or NaHMDS, R-X, THF; b) 2.5N NaOH, MeOH; c) 2.2.1-Boc, DMF, DIPEA, Bop; d) H₂NOH*HCI, MeOH, NH_zOH(aq); e) pyranona, DCM, Na(OAc)₃BH; f) 4N HCI/1,4-dioxane; g) cond a or cond b or cond c cond a = Ar-X, DMSO, Et₃N, 120 °C cond b - Ar-X, Cs₂CO₃, XantPhos, Pd₂(dba)₃, dioxane, DMSO cond c = Ar-X, 1,4-dioxane, Et₃N, 100 °C

Example 24

1,5-anhydrO-2,3-dideoxy-3-{[(1R,3S )-3-(2,2-difluoroethyl)-3-({(1S,4S )-5-(6(trifluoromethyl)pyrimidin-4-yl]-2,5-diazabicyclo[2.2.1]hept-2yl}carbonyl)cyclopentyl]amino}-4-O-methyl-D-erythro-pentitol

Step 1 : Préparation of 2,2-difluoroethvl trifluoromethanesulfonate ^fV^o^xScf₃

Triflic anhydride (27.9g, 99.1mmol) was placed in a flask and cooled with an ice bath. 2,2-Difluoroethanol (8.1g, 99.1mmol) was added and the reaction was heated to 84°C for 1hour. The reaction was cooled in an ice bath and poured into 100ml cold 5%NaHCO₃ solution. The mixture was extracted with diethyl ether, dried over MgSO₄, and concentrated under reduced pressure to remove the ether. The residue was vaccuum distilled to give 2,2-difluoroethyl trifluoromethanesulfonate as a clear liquid (13.6g, 64%, bp~55°C). ¹H NMR (400 MHz, CDCI₃) d ppm 6.17-5.88 (1H), 4.61-4.13 (2H)

Step 2: Préparation of methyl (1S,4S)-1-(2,2-difluoroethvl)-4-(2,5-dimethvl-1Hpyrrol-1-vl)cvclopent-2-ene-1-carboxylate

A -40°C solution of 2M (in Ethylbenzene/THF/Heptane) LDA (36ml, 72mmol) in THF (80ml) was placed in flask with THF (80ml) was treated with a solution of methyl (1 R,4S)-4-(2,5-dimethyl-1 H-pyrrol-1-yl)cyclopent-2-ene-1-carboxylate (7.9g, 36.3mmol) in THF (17ml) while keeping the température less than -32°C. The reaction was stirred for 30min and then 2,2-difluoroethyl trifluoromethanesulfonate was added slowly, keeping the température <-28°C. The reaction was stirred with cold bath in place and allow to slowly warm. After 4 hours the reaction was poured into NH4CI solution and extracted twice with ethyl acetate. The combined organics were washed with brine, dried over MgSO₄ and concentrated to give a brown oil. The oil was passed through a column of silica gel with 10% ethyl acetate/hexanes to give methyl (1S,4S)-1-(2,2-difluoroethyl)-4(2,5-dimethyl-1 H-pyrrol-1-yl)cyclopent-2-ene-1-carboxylate as a brown oil. (7.5g, 73%). ¹H NMR (400 MHz, CDCI₃) d ppm 6.07-6.04 (1H), 6.02-6.00 (0.25H) 5.985.96 (1H), 5.88-5.86 (0.5H), 5.75-5.72 (2.25H), 5.36-5.30 (1H), 3.74 (3H), 2.532.41 (2H), 2.39-2.22 (2H), 2.19 (6H)

Step 3: Préparation of (1S,4S)-1-(2,2-difluoroethyl)-4-(2.5-dimethyl-1H-pvrrol-1yl)cyclopent-2-ene-1 -carboxylic acid

A solution of methyl (1S,4S)-1-(2,2-difluoroethyl)-4-(2,5-dimethyl-1 H-pyrrol-1yl)cyclopent-2-ene-1-carboxylate (7.54g, 26.6mmol) in methanol (60ml) was treated with 2.5N NaOH (15ml, 37.5mmol) and stirred at room température for 22 hours. The methanol was removed under reduced pressure and the residue partitioned between diethyl ether and water. The layers were separated and the aqueous was acidified with 4N HCl, extracted with ethyl acetate, washed with brine, dried over MgSO₄, and concentrated under reduced pressure to give (1 S,4S )-1-(2,2-difluoroethyl )-4-(2,5-dimethyl-1H-pyrrol-1-yl)cyclopent-2-ene-1carboxylic acid as a brown oil (6.59g, 92%). ¹H NMR (400 MHz, CDCI₃) d ppm 6.12-6.09 (1H), 6.07-6.05 (0.25H), 5.99-5.96 (1H), 5.93-5.91 (0.5H), 5.79-5.76 (0.25H), 5.74 (2H), 5.38-5.32 (1H), 2.54-2.46 (2H), 2.43-2.25 (2H), 2.20 (6H).

Step 4: Préparation oftert-butyl (1S,4S)-5-ffî1S,4S)-1-(2,2-difluoroethyl)-4-(2,5dimethvl-1 H-pvrrol-1-vl)cvclopent-2-en-1-vncarbonyl}-2,5diazabicvclo[2.2.11heptane-2-carboxvlate

BOP= benzotriazol-l-yloxytris(dimethylamino) phosphoniumhexafluorophosphate

A solution of (1S,4S)-1-(2,2-difluoroethyl)-4-(2,5-dimethyl-1H-pyrrol-1yl)cyclopent-2-ene-1-carboxylîc acid (6.12g, 22.6mmol) in DMF (45ml) was treated with N,N-diisopropylethylamine (11.7ml, 67.2mmol) and BOP (11.93g, 27.0mmol) and stirred under nitrogen at room température for 40 minutes. The (1S,4S)-2-BOC-2,5-diazabicyclo[2.2.1]heptane (4.46g,22.5mmol) was added and the reaction was stirred for 18 hours.The reaction was diluted with ethyl acetate (100ml) and washed with 60% saturated NaHCO₃ (125ml). The aqueous layer was extracted with ethyl acetate. The organic layers were combined, washed with brine, dried over MgSO₄ and concentrated under reduced pressure to give a brown oil (16.8g). The oil was passed through a column of silica gel with 50% ethyl acetate/hexanes to give tert-butyl (1S,4S)-5-{[(1S,4S)-1-(2,2-difluoroethyl)4-(2,5-dimethyl-1 H-pyrrol-1 -yl)cyclopent-2-en-1 -yl]carbonyl}-2,5diazabicyclo[2.2.1]heptane-2-carboxylate as a brown foam (7.86g, 78%). ¹H NMR (400 MHz, CDCI₃) d ppm 6.25-6.19 (1H), 6.04-5.96 (1.25H), 5.85-5.81 (0.5H), 5.74-5.68 (2.25H), 5.37-5.25 (1H), 4.98-4.43 (2H), 3.73-3.29 (4H), 2.772.29 (2H), 2.26-2.09 (8H), 1.93-1.67 (2H), 1.46-1.34 (9H).

Step 5: Préparation of tert-butyl (1S,4S)-5-([(1S,4S)-4-amino-1-(2,2difIuoroethvl)cvclopent-2-en-1-vl1carbonvl)-2,5-diazabicvclof2.2.11heptane-2carboxylate

A solution of tert-butyl (1S,4S)-5-{[(1S,4S)-1-(2,2-difluoroethyl)-4-(2,5-dimethyl1H-pyrrol-1-yl)cyclopent-2-en-1-yl]carbonyl}-2,5-diazabicyclo[2.2.1]heptane-2carboxylate (7.86g, 17.5mmol) in methanol (100ml) and water (30ml) was treated with hydroxylamine hydrochloride (6.35g, 91.0mmol) and 50 weight% solution of hydroxylamine (5.0ml, 81.6mmol). The reaction was stirred under nitrogen and heated to 66°C for 39 hours. The reaction was cooled to room température, diluted with water, and made basic (pH~11 ) by the addition of 2.5N NaOH. The reaction mixture was extracted with ethyl acetate (3x150ml), washed with brine, dried over MgSÛ4 and concentrated under reduced pressure to give a crude mixture of tert-butyl ( 1S,4S)-5-{[(1 S,4S)-4-amino-1-(2,2-difluoroethyl)cyclopent-2en-1-yl]carbonyl}-2,5-diazabicyclo[2.2.1]heptane-2-carboxylate as a brown oil (9.12g, theoretical yield 6.50g) which was used without further purification in the next step.

Step 6: Préparation of tert-butyl (1S,4S)-5-fl(1S,3R)-3-amino-1-(2,2difluoroethvl)cvclopentyl1carbonvl}-2,5-diazabicvclo[2.2.11heptane-2-carboxylate

A mixture of tert-butyl (1S,4S)-5-{[(1S,4S)-4-amino-1-(2,2-difluoroethyl)cyclopent2-en-1-yl]carbonyl}-2,5-diazabicyclo[2.2.1]heptane-2-carboxylate (6.50g, 17.5mmol) and 5% palladium on carbon in methanol (100ml) was stirred at room température under 46psi of hydrogen for 21 hours. The reaction was filtered through celîte and the filter cake washed with methanol. The filtrate and washings were concentrated under reduced pressure to give tert-butyl (1S,4S)-5-{[(1S,3R)3-amino-1-(2,2-difluoroethyl)cyclopentyl]carbonyl}-2,5diazabicyclo[2.2.1]heptane-2-carboxylate as a brown oil (6.67g, theoretical yield 6.54g) which was used without further purification in the next step.

Step 7: Préparation of1,5-anhvdro-3-ffî1R,3S)-3-([(1S,4S)-5-(tertbutoxvcarbonvl)-2.5-diazabicvclo[2.2.1lhept-2-vllcarbonvl)-3-(2,2difluoroethvl)cvclopentvl1aminoÎ-2₁3-dideoxv-4-Q-methyl-D-ervthro-pentitol

A 0°C solution of tert-butyl (1S,4S)-5-{[(1S,3R)-3-amino-1-(2,2difluoroethyl)cyclopentyl]carbonyl)-2,5-diazabicyclo[2.2.1]heptane-2-carboxylate (3.45g, 9.2mmol) in dichloromethane (50ml) was treated with sodium triacetoxyborohydride (5.34g, 25.2mmol) and (3R)-3-methoxytetrahydro-4Hpyran-4-one (2.31g, 17.8mmol). The reaction was stirred under nitrogen at 0°C for 30 minutes then allowed to warm to room température and stirred for 47 hours. The reaction was treated with 2.5N NaOH (35mL) and stirred for 10 minutes. The reaction was diluted with water and the layers separated. The aqueous layer was extracted twice with ethyl acetate. The dichloromethane layer was concentrated under reduced pressure and partitioned between ethyl acetate and water. The organic layers were combined, washed with brine, dried over MgSO₄, concentrated under reduced pressure and purified with the Biotage (ΟΙ 00% methanol/ethyl acetate, 15 column volumes) to give1,5-anhydro-3{[( 1 R,3S)-3-{[( 1 S,4S )-5-(tert-butoxycarbonyl)-2,5-diazabicyclo[2.2.1 ]hept-2yl]carbonyl)-3-(2,2-difluoroethyl)cyclopentyl]amino}-2,3-dideoxy-4-O-methyl-Derythro-pentitol (2.13g, 47%) which was used without further purification in the next step.

Step 8: Préparation of 1.5-anhvdro-2,3-dideoxv-3-{[(1R,3S)-3-{((1S,4S)-2,5diazabicvctof2.2Jlhept-2-vl1carbonvn-3-(2.2-difluoroethvl)cvclopentvllaminoÎ-4O-methvl-D-ervthro-pentitol (HCl sait)

To a solution of 1,5-anhydro-3-{[(1R,3S)-3-{[(1S,4S)-5-(tert-butoxycarbonyl)-2,5diazabicyclo[2.2.1]hept-2-yl]carbonyl}-3-(2,2-difluoroethyl)cyclopentyl]amino}-2,3dideoxy-4-O-methyl-D-erythro-pentitol (2.13g, 4.4mmol) in 1,4-dioxane (20ml) was added 4N HCI/1,4-dioxane (20ml). The reaction was stirred at room température for 16 hours. The liquid was decanted leaving a gummy solid which was dissolved in methanol and concentrated under reduced pressure. The residue was dissolved in methanol/methylene chloride and concentrated to give the HCl sait of 1,5-anhydro-2,3-dideoxy-3-{[(1R,3S)-3-{[(1S,4S)-2,5diazabicyclo[2.2.1]hept-2-yl]carbonyl}-3-(2,2-difluoroethyl)cyclopentyl]amino}-4O-methyl-D-erythro-pentitol_as a brown foam (2.23g, theoretical yield 1.85g) which was used in the next step without further purification.

Step 9: Préparation of 1,5-anhydro-2,3-dideoxv~3-f((1R,3S)-3-(2,2-difluoroethvlÎ3-(((1 S.4S)-5-f6-(trifluoromethYl)pyrimidin-4-vll-2.5-diazabicvclor2.2.1lhept-2vl}carbonvl)cvclopentvl1aminoÎ-4-O-methyl-D-ervthro-pentitol

The HCl sait of 1,5-anhydro-2,3-dideoxy-3-{[(1 R,3S)-3-{[(1S,4S )-2,5diazabicyclo[2.2.1]hept-2-yl]carbonyl}-3-(2,2-difluoroethyl)cyclopentyl]amino}-4O-methyl-D-erythro-pentitol (177mg, 0.42mmol) was placed in a flask with 1,4dioxane (3ml). To the stirred solution was added Xantphos (36mg, 0.062mmol), Pd₂(dba)₃ (22mg, 0.024mmol), Cs₂CO₃ (350mg, 1.07mmol), and 4-chloro-6(trifluoromethyl)pyrimidine (195mg, 1.07mmol) dissolved in 1,4-dioxane (1ml). DMSO (0.5ml) was added for solubility and the reaction was heated to 100°C for 21.5 hours. The reaction was allowed to cool to room température and filtered through celite. The filtrate was concentrated under reduced pressure and purified with the Biotage (0-100% methanol/ethyl acetate 15 column volumes) to give 1,5anhydro-2,3-dideoxy-3-{[(1R,3S )-3-(2,2-difluoroethyl)-3-({(1 S,4S)-5-[6(trifluoromethyl)pyrimidin-4-yl]-2,5-diazabicyclo[2.2.1]hept-2yl}carbonyl)cyclopentyl]amino}-4-O-methyl-D-erythro-pentitol as a whitefoam (43mg, 20%). LC/MS (M+H) = 534.2504 exp, 534.2597 obs; ¹H NMR (400 MHz, CDCI₃) d ppm 8.62 (1H), 6.74-6.46 (1H), 5.93-5.62 (1H), 5.29-4.50 (2H), 4.084.03 (1 H), 3.93-3.88 (1 H), 3.77-3.55 (2H), 3.52-3.32 (6H), 3.30-3.21 (3H), 2.762.65 (1 H), 2.51-2.33 (1H), 2.21-1.84 (7H), 1.70-1.40 (5H)

Example 25

1,5-anhydro-2,3-dideoxy-3-{[(1 R,3S )-3-(2,2-difluoroethyl)-3-({(1S,4S)-5-[4 (trifluoromethyl)pyndm-2-ylj-2,5-diazabicyclo[2.2.1 ]hept-2yl}carbonyl)cyclopentyl]amino}-4-O-methyl-D-erythro-pentitol

Prepared as descrïbed in example 24 using 2-chloro-4-(trifluoromethyl)pyridine in place of 4-chloro-6-(trifluoromethyl)pyrimidine in step 9. LC/MS (M+H) = 533.2551 exp, 533.2651 obs; ¹H NMR (400 MHz, CDCI₃) d ppm 8.24-8.20 (1H), 6.74-6.73 (1H), 6.49-6.44 (1H), 5.95-5.57 (1H), 5.07-4.73 (2H), 4.06-4.01 (1H), 3.93-3.87 (1H), 3.70-3.49 (2H), 3.45-3.30 (6H), 3.28-3.20 (3H), 2.77-2.64 (1H), 2.53-2.36(1 H), 2.23-1.81 (7H), 1.73-1.41 (5H)

Example 26

1,5-anhydro-2,3-dideoxy-3-{[(1 R,3S)-3-(2,2-difluoroethyl)-3-({(1 S,4S)-5-[2(trifluoromethyl)pyridin-4-yl]-2,5-diazabicyclo[2.2.1]hept-2yl}carbonyl)cyclopentyl]amino}-4-O-methyl-D-erythro-pentitol

Prepared as described in example 24 using 4-iodo-2-(trifluoromethyl)pyridine in place of 4-chloro-6-(trifluoromethyl)pyrimidine in step 9. LC/MS (M+H) = 533.2551 exp, 533.2598 obs; ¹H NMR (400 MHz, CDCI₃) d ppm 8.29-8.27 (1H), 6.74-6.70 (1 H), 6.50-6.43 (1 H), 5.93-5.59 (1 H), 5.09-4.74 (1 H), 4.54 (1 H), 4.074.02 (1 H), 3.93-3.88 (1 H), 3.68-3.48 (2H), 3.39-3.20 (7H), 2.74-2.62 (1 H), 2.512.37 (1H), 2.19-1.77 (7H), 1.70-1.40 (5H)

Example 27

1,5-anhydro-2,3-dideoxy-3-{[(1R,3S)-3-(2,2-difluoroethyl)-3-({(1S,4S)-5-[4(trifluoromethyl)pyrimidin-2-yl]-2,5-diazabicyclo[2.2.1]hept-2yl}carbonyl)cyclopentyl]amino}-4-O-methyl-D-erythro-pentitol

Prepared as described in example 24 using 2-chloro-4-(trifluoromethyl)pyrimidine in place of 4-chloro-6-(trifluoromethyl)pyrimidine and modifying step 9 as follows. The HCl sait of 1,5-anhydro-2,3-dideoxy-3-{[(1R,3S)-3-{[(1S,4S)-2,5diazabicyclo[2.2.1]hept-2-yl]carbonyl}-3-(2,2-difluoroethyl)cyclopentyl]amino}-4O-methyl-D-erythro-pentitol (175mg, 0.41mmol) was placed in flask with 1,4dioxane (3ml). Triethylamine (0.2ml, 1.43mmol) was added as well as 2-chloro-4(trifluoromethyl)pyrimidine (237mg, 1.30mmol). DMSO (0.3ml) was added for solubility and the reaction was heated to 100°C for 14.5 hours. The reaction was cooled to room température and partitioned between ethyl acetate and water. The aqueous layer was extracted with ethyl acetate and the combined organics were washed with brine, dried over MgSO4, concentrated under reduced pressure and purified with the Biotage (0-100% methanol/ethyl acetate 15 column volumes) to give 1,5-anhydro-2,3-dideoxy-3-{[(1R,3S)-3-(2,2difluoroethyl)-3-({(1S,4S)-5-[4-(trifluoromethyl)pyrimidin-2-yl]-2,5diazabicyclo[2.2.1]hept-2-yl}carbonyl)cyclopentyl]amino}-4-O-methyl-D-erythropentitol as a brown foam (126mg, 58%). LC/MS (M+H) - 534.2504 exp, 534.2452 obs; ¹H NMR (400 MHz, CDCI₃) d ppm 8.48-8.45 (1H), 6.79-6.78 (1 H), 5.96-5.58 (1H), 5.10-4.71 (2H), 4.07-4.01 (1H), 3.93-3.87 (1H), 3.69-3.55 (4H), 3.39-3.32 (4H), 3.30-3.21 (3H), 2.77-2.66 (1H), 2.52-2.40 (1H), 2.23-1.82 (7H), 1.70-1.39 (5H)

Example 28

1,5-anhydro-2,3-dideoxy-3-{[(1R,3S)-3-(2,2-difluoroethyl)-3-({(1S,4S)-5-[6(trifluoromethyl)pyridin-2-yl]-2,5-diazabicyclo[2.2.1]hept-2yl}carbonyl)cyclopentyl]amino}-4-O-methyl-D-erythro-pentitol

Prepared as described in example 24 using 2-chloro-6-(trifluoromethyl)pyridine in place of 4-chloro-6-(trifluoromethyl)pyrimidine and modifying step 9 asfollows. To a solution of HCl sait of 1,5-anhydro-2,3-dideoxy-3-{[(1 R,3S)-3-{[(1S,4S )-2,5diazabicyclo[2.2.1]hept-2-yl]carbonyl}-3-(2,2-difluoroethyl)cyclopentyl]amino}-4O-methyl-D-erythro-pentitol (183mg, 0.43mmol) in DMSO (2ml) was added triethylamine (0.2ml, 1.43mmol) and 2-chloro-6-(trifluoromethyl)pyridine (179mg, 1.05mmol). The reaction was heated to 120 ^qC for 14.5 hours. The reaction was cooled to room température, and added to stirring ice water. The mixture was extracted with ethyl acetate(3x). The combined organics were washed with brine, dried over MgSO₄ concentrated under reduced pressure and purified with the Biotage (0-100% methanol/ethyl acetate, 15 column volumes) to give 1,5anhydro-2,3-dideoxy-3-{[(1R,3S)-3-(2,2-difluoroethyl)-3-({(1S,4S)-5-[6(trifluoromethyl)pyridin-2-yl]-2,5-diazabicyclo[2.2.1]hept-2yl}carbonyl)cyclopentyl]amino}-4-O-methyl-D-erythro-pentitol as a brown foam (58mg 28%). LC/MS (M+H) = 533.2551 exp, 533.2546 obs; ¹H NMR (400 MHz, CDCI₃) d ppm 7.54-7.51 (1H), 6.91-6.89 (1H), 6.51-6.38 (1H), 5.94-5.54 (1 H), 5.11-4.70 (2H), 4.06-4.01 (1H), 3.93-3.87 (1H), 3.70-3.53 (2H),

3.51- 3.31 (6H), 3.27-3.20 (3H), 2.78-2.61 (1H), 2.53-2.36 (1 H), 2.20-1.80 (7H),

1.68-1.38 (5H)

Example 29

1,5-anhydro-2,3-dideoxy-3-{[(1R,3S)-3-(2,2-difluoroethyl)-3-({(1S,4S)-5-[6(trifluoromethyl)pyrazin-2-yl]-2,5-diazabicyclo[2.2.1]hept-2yl}carbonyl)cyclopentyl]amino}-4-O-methyl-D-erythro-pentitol

Prepared as described in example 24 using 2-iodo-6-(trifluoromethyl)pyrazine in place of 4-chloro-6-(trifluoromethyl)pyrimidine in step 9. LC/MS (M+H) = 534.2504 exp, 534.2548 obs; ¹H NMR (400 MHz, CDCI₃) d ppm 8.13 (1 H), 8.017.93 (1H), 5.92-5.57 (1H), 5.09-4.75 (2H), 4.06-4.01 (1 H), 3.92-3.87 (1H), 3.683.48 (4H), 3.38-3.30 (4H), 3.28-3.20 (3H), 2.74-2.63 (1 H), 2.51-2.32 (1H), 2.211.83 (7H), 1.75-1.42 (5H)

Example 30

1,5-anhydro-2,3-dideoxy-3-{[(1R,3S)-3-(2,2-difluoroethyl)-3-({(1S,4S)-5-[2(trifluoromethyl)pyrimidin-4-yl]-2,5-diazabicyclo[2.2.1]hept-2yl}carbonyl)cyclopentyl]amino}-4-O-methyl-D-erythro-pentitol

Prepared as described in example 24 using 4-chloro-2-(trifluoromethyl)pyrimidine in place of 4-chloro-6-(trifluoromethyl)pyrimidine in step 9. LC/MS (M+H) = 534.2504 exp, 534.2521 obs; ¹H NMR (400 MHz, CDCI₃) d ppm 8.28-8.26 (1H), 6.52-6.24 (1H), 5.93-5.62 (1H), 5.33-4.38 (2H), 4.08-4.03 (1H), 3.94-3.89 (1 H), 3.71-3.57 (2H), 3.49-3.33 (6H), 3.29-3.21 (3H), 2.75-2.66 (1 H), 2.50-2.36 (1 H), 2.22-1.86 (7H), 1.69-1.41 (5H)

Example 31

1,5-anhydro-2,3-dideoxy-3-{[(1R,3S)-3-(2₁2-difluoroethyl)-3-({(1S,4S)-5-[5( trifluoromethyl )pyridazin-3-yl]-2,5-diazabicyclo[2.2.1]hept-2yl}carbonyl)cyclopentyl]amino}-4-O-methyl-D-erythro-pentitol

Prepared as described in example 27 using 3-chloro-5-(trifluoromethyl)pyridazine in place of 2-chloro-4-(trifluoromethyl)pyrimidine in step 9. LC/MS (M+H) = 534.2504 exp, 534.2531 obs; ¹H NMR (400 MHz, CDCI₃) d ppm 8.76 ( 1 H), 6.746.68 (1H), 5.96-5.90 (1H), 5.37-4.84 (2H), 4.10-4.04 (1H), 3.96-3.90 (1H), 3.813.54 (3H), 3.47-3.34 (4H), 3.32-3.22 (4H), 2.77-2.67 (1H), 2.51-2.37 (1H), 2.221.86 (7H), 1.72-1.46 (5H)

Example 32

1,5-anhydro-2,3-dideoxy-4-O-methyl-3-{[(1R,3S)-3-(2,2,2-trifluoroethyl)-3({(1S,4S)-5-[6-(trifluoromethyl)pyrimidin-4-yl]-2,5-diazabicyclo[2.2.1]hept-2yl)carbonyl)cyclopentyl]amino}-D-erythro-pentitol

Prepared as described in example 24 using 3,3,3-trifluoroethanol in place of 2,2difluoroethanol in step 1. LC/MS (M+H) = 552.2409 exp, 552.2432 obs; ¹H NMR (400 MHz, CDCI₃) d ppm 8.61 (1H), 6.70-6.45 (1H), 5.26-5.21 (1H), 5.08-4.96 (1H), 4.08-4.03 (1H), 3.92-3.87 (1H), 3.68-3.61 (2H), 3.53-3.44 (1H), 3.42-3.20 (8H), 2.72-2.65 (1 H), 2.56-2.27 (3H), 2.08-1.88 (5H), 1.80-1.47 (5H)

Example 33

1,5-anhydro-2,3-dideoxy-4-O-methyl-3-{[(1R,3S)-3-(2,2,2-trifluoroethyl)-3({(1S,4S)-5-[4-(trifluoromethyl)pyridin-2-yl]-2,5-diazabicyclo[2.2.1]hept-2yl}carbonyl)cyclopentyl]amino}-D-erythro-pentitol

Prepared as described in example 24 using 3,3,3-trifluoroethanol in place of 2,2difluoroethanol in step 1 and using 2-chloro-4-(trifluoromethyl)pyridine in place of

2-chloro-6-(trifluoromethyl)pyridine in step 9. LC/MS (M+H) = 551.2457 exp, 551.2703 obs; ¹H NMR (400 MHz, CDCI₃) d ppm 8.22-8.21 (1H), 6.74-6.73 (1H), 6.47-6.43 (1H), 5.06-4.71 (2H), 4.06-4.01 (1H), 3.92-3.87 (1H), 3.65-3.50 (3H), 3.40-3.20 (8H), 2.72-2.63 (1H), 2.53-2.27 (3H), 2.08-1.82 (5H), 1.78-1.45 (5H)

Example 34

1,5-anhydro-2,3-dideoxy-4-O-methyl-3-{[(1R,3S)-3-(2,2,2-trifluoroethyl)-3({(1S,4S)-5-[2-(trifluoromethyl)pyridin-4-yl]-2,5-diazabicyclo[2.2,1]hept-2yl}carbonyl)cyclopentyl]amino}-D-erythro-pentitol

Prepared as described in example 24 using 3,3,3-trifluoroethanol in place of 2,2difluoroethanol in step 1 and using 4-iodo-2-(trifluoromethyl)pyridine in place of 2chloro-4-(trifluoromethyl)pyrimidine in step 9. LC/MS (M+H) = 551.2457 exp, 551.2610 obs; ¹H NMR (400 MHz, CDCI₃) d ppm 8.26-8.25 (1H), 6.71-6.68 (1H), 6.46-6.42 (1H), 5.09-4.83 (1H), 4.55-4.52 (1H), 4.05-4.01 (1H), 3.90-3.85 (1H), 3.66-3.48 (3H), 3.36-3.18 (8H), 2.70-2.62 (1H), 2.51-2.27 (3H), 2.06-1.87 (5H), 1.80-1.45 (5H)

Example 35

1,5-anhydro-2,3-dideoxy-4-O-methyl-3-{[(1 R,3S)-3-(2,2,2-trifluoroethyl)-3({(1S,4S)-5-[4-(trifluoromethyl)pyrimidin-2-yl]-2,5-diazabicyclo[2.2.1]hept-2yl}carbonyl)cyclopentyl]amino}-D-erythro-pentitol

Prepared as described in example 27 using 3,3,3-trifluoroethanol in place of 2,2difluoroethanol in step 1. LC/MS (M+H) = 552.2409 exp, 551.2426 obs; ¹H NMR (400 MHz, CDCI₃) d ppm 8.50-8.46 (1H), 6.81-6.80 (1H), 5.11-4.74 (2H), 4.134.06 (1H), 3.97-3.92 (1H), 3.67-3.59 (4H), 3.41-3.25 (7H), 2.90-2.75 (1H), 2.542.33 (3H), 2.07-1.88 (5H), 1.79-1.58 (5H)

Example 36

1,5-anhydro-2,3-dideoxy-4-O-methyl-3-{[(1R,3S)-3-(2,2,2-trifluoroethyi)-3({(1S,4S)-5-[6-(trifluoromethyl)pyridin-2-yl]-2,5-diazabicyclo[2.2.1]hept-2yl}carbonyl)cyclopentyl]amino}-D-erythro-pentitol

Prepared as described in example 27 using 3,3,3-trifluoroethanol in place of 2,2difluoroethanol in step 1 and using 2-chloro-6-(trifluoromethyl)pyridine in place of 2-chloro-4-(trifluoromethyl)pyrimidine in step 9.. LC/MS (M+H) = 551.2457 exp, 551.2581 obs; ¹H NMR (400 MHz, CDCI₃) d ppm 7.55-7.51 (1 H), 6.92-6.90 (1 H), 6.47-6.40 (1H), 5.12-4.70 (2H), 4.07-4.03 (1H), 3.93-3.88 (1H), 3.72-3.47 (3H), 3.40-3.31 (5H), 3.28-3.20 (3H), 2.73-2.64 (1H), 2.53-2.25 (3H), 2.10-1.80 (5H), 1.77-1.44 (5H)

9l

Example 37

1,5-anhydro-2,3-dideoxy-4-O-methyl-3-{[(1R,3S)-3-(2,2,2-trifluoroethyl)-3({(1S,4S)-5-[6-(trifluoromethyl)pyrazin-2-yl]-2,5-diazabicyclo[2.2.1]hept-2yl}carbonyl)cyclopentyl]amino}-D-erythro-pentitol

Prepared as described in example 24 using 3,3,3-trifluoroethanol in place of 2,2difluoroethanol in step 1 and using 1-iodo-6-(trifluoromethyl)pyrazine in place of 4-chloro-6-(trifIuoromethyl)pyrimidine in step 9. LC/MS (M+H) = 552.2409 exp, 552.2443 obs; ¹H NMR (400 MHz, CDCI₃) d ppm 8.16 (1 H), 8.01-7.96 (1 H), 5.09-4.96 (2H), 4.09-4.04 (1 H), 3.94-3.89 (1 H), 3.68-3.57 (3H), 3.53-3.49 (1 H), 3.39-3.23 (7H), 2.74-2.67 (1H), 2.54-2.29 (3H), 2.09-1.90 (5H), 1.81-1.49 (5H)

Example 38

1,5-anhydro-2,3-dîdeoxy-4-0-methyl-3-{[(1R,3S)-3-(2,2,2-trifluoroethyl)-3({(1S,4S)-5-[2-(trifluoromethyl)pyrimidin-4-yl]-2,5-diazabicyclo[2.2.1]hept-2yl}carbonyl)cyclopentyl]amino}-D-erythro-pentitol

Prepared as described in example 24 using 3,3,3-trifluoroethanol in place of 2,2difluoroethanol in step 1 and using 4-chloro-2-(trifluoromethyl)pyrimidine in place of 4-chloro-6-(trifluoromethyl)pyrimidine in step 9. LC/MS (M+H) = 552.2409 exp, 552.3073 obs; ¹H NMR (400 MHz, CDCI₃) d ppm 8.23-8.21 (1H), 6.49-6.22 (1H), 5.25-4.93 (2H), 4.04-3.99 (1H), 3.89-3.83 (1H), 3.65-3.57 (2H), 3.46-3.17 (9H), 2.68-2.62 (1 H), 2.52-2.26 (3H), 2.05-1.85 (5H), 1.76-1.43 (5H)

Example 39 ^FX M

1,5-anhydro-2₍3-dideoxy-4-O-methyl-3-{[(1R,3S)-3-(2,2,2-trifluoroethyl)-3({(1S,4S)-5-[5-(trifluoromethyl)pyridazin-3-yl]-2,5-diazabicyclo[2.2.1]hept-2yl}carbonyl)cyclopentyl]amino}-D-erythro-pentitol

Prepared as described in example 27 using 3,3,3-trifluoroethanol in place of 2,2difluoroethanol in step 1 and using 3-chloro-5-(trifluoromethyl)pyridazine in place of 2-chloro-4-(trifluoromethyl)pyrimidine in step 9. LC/MS (M+H) = 552.2409 exp, 552.2344 obs; ¹H NMR (400 MHz, CDCI₃) d ppm 8.72 (1 H), 6.72-6.64 (1H), 5.32-4.82 (2H), 4.06-4.01 (1 H), 3.91-3.86 (1 H), 3.78-3.53 (3H), 3.42-3.18 (8H), 2.69-2.64 (1 H), 2.52-2.24 (3H), 2.07-1.84 (5H), 1.76-1.44 (5H)

Example 40

1,5-anhydro-2,3-dideoxy-3-{[(1S,4S)-4-isopropyll-4-({(1S,4S)-5-[4(trifluoromethyl)pyridin-2-yl]-2,5-diazabicyclo[2.2.1]hept-2-yl}carbonyl)cyclopent2-en-1-yl]amino)-4-O-methyl-D-erythro-pentitol

Prepared as described in example 1 starting with (1S,4S)-4-(2,5-dimethyl-1Hpyrrol-1-yl)-1-isopropylcyclopent-2-ene-1-carboxylic acid and skipping steps 1, 2 and 5. LC/MS (M+H) = 509.2739 exp, 509.2724 obs; ¹H NMR (400 MHz, CDCI- ₃) d ppm 8.19(1 H), 6.72-6.68 (1 H), 6.46-6.38 (1 H), 5.90-5.75 (2H), 5.09-4.82 (2H), 4.02-3.12 (13H), 2.86-2.67 (1H), 2.46-2.16 (1H), 2.08-1.47 (7H), 0.84-0,64 (6H)

Example 41

1,5-anhydro-2,3-dideoxy-3-{[(1S,4S)-4-isopropyll-4-({(1S,4S)-5-[4(trifluoromethyl)pyrimidin-2-yl]-2,5-diazabicyclo[2.2.1]hept-2yl)carbonyl)cyclopent-2-en-1-yl]amino}-4-O-methyl-D-erythro-pentitol

Prepared as described in example 2 starting with ( 1S,4S )-4-(2,5-dimethyl-1Hpyrrol-1-yl)-1-isopropylcyclopent-2-ene-1-carboxylic acid and skipping steps 1,2 and 5. LC/MS (M+H) = 510.2692 exp, 510.2926 obs; ¹H NMR (400 MHz, CDCI- ₃) d ppm 8.43-8.42 (1H), 6.75-6.74 (1H), 5.90-5.77 (2H), 5.09-4.80 (2H), 4.01-

3.16 (13H), 2.86-2.69 (1H), 2.45-2.17 (1H), 2.08-1.30 (7H), 0.82-0.66 (6H)

Example 42

1,5-anhydro-2,3-dideoxy-3-{[(1S,4S)-4-isopropyll-4-({(1S,4S)-5-[6(trifluoromethyl)pyridin-2-yl]-2,5-diazabicyclo[2.2.1]hept-2-yl}carbonyl)cyclopent2-en-1-yl]amino}-4-O-methyl-D-erythro-pentitol

Prepared as described in example 1 starting with (1 S,4S )-4-(2,5-dimethyl-1Hpyrrol-1-yl)-1-isopropylcyclopent-2-ene-1-carboxylic acid and skipping steps 1,2 and 5. LC/MS (M+H) = 509.2739 exp, 509.2680 obs; ¹H NMR (400 MHz, CDCI3) d ppm 7.55-7.52 (1H), 6.94-6.89 (1H), 6.52-6.40 (1H), 5.96-5.78 (2H), 5.124.84 (2H), 4.07-3.16 (13H), 2.90-2.69 (1H), 2.49-2.21 (1H), 2.12-1.42 (7H), 0.870.68 (3H)

Example 43

1,5-anhydro-2,3-dideoxy-3-{[(1 S,4S)-4-isopropyll-4-({(1 S,4S)-5-[6(trifluoromethyl)pyrazin-2-yl]-2,5-diazabicyclo[2.2.1]hept-2-yl)carbonyl)cyclopent2-en-1-yl]amino}-4-O-methyl-D-erythro-pentitol

Prepared as described in example 3 starting with (1S,4S)-4-(2,5-dimethyl-1Hpyrrol-1-yl)-1-isopropylcyclopent-2-ene-1-carboxylic acid and skipping steps 1 and 2, skipping step 5, using (3R)-3-methoxytetrahydro-4H-pyran-4-one in step 6, and 2-chloro-6-(trifluoromethyl)pyrazine in place of 4-chloro-6(trifluoromethyl)pyrimidine in step 8. LC/MS (M+H) = 510.2692 exp, 510.2699 obs; ¹H NMR (400 MHz, CDCI₃) d ppm 8.15 (1 H), 8.04-7.95 (1 H), 5.93-5.82 (2H), 5.17-4.91 (2H), 4.16-3.18 (13H), 2.91-2.69 (1H), 2.48-2.21 (1 H), 2.10-1.52 (7H), 0.85-0.67 (6H)

Example 44

1,5-anhydro-3-{[(1R,3S)-3-{[(1S,4S)-5-(tert-butoxycarbonyl)-2,5diazabicyclo[2.2.1]hept-2-yl]carbonyl}-3-(2,2,2-trifluoroethyl)cyclopentyl]amino}2,3-dideoxy-4-O-methyl-D-erythro-pentitol

Prepared as described in example 24 using 2,2,2-trifluoroethanol in place of 2,2difluoroethanol in step 1 and skipping steps 8 and 9. LC/MS (M+H) = 506.2842 exp, 506.3033 obs; ¹H NMR (400 MHz, CDCI₃) d ppm 4.86-4.65 (1H), 4.52-4.38 (1H), 4.09-4.03 (1H), 3.93-3.87 (1H), 3.68-3.23 (11 H), 2.73-2.68 (1H), 2.55-2.24 (3H), 2.10-2.03(1 H), 1.96-1.89 (1 H), 1.85-1.59 (7H), 1.54-1.47 (1 H), 1.44-1.39 (9H)

Scheme 3. Préparation of Examples 45-59

a) NaOH, MeOH, H₂0; b) BnOH, EDC, DMAP; c) LDA, R|COR₂; d) NH₂OH*HCl, NH₂OH*H₂O, MeOH; e) MeO-pyranone, Na(OAc)₂BH; f) TFAA; g) AcCl, pyridine; h)

H₂, Pt/C; i) (COCl)₂; j) arylpiperazine; k) K₂COî, MeOH; l) NaBHi; m) HCI/dioxane; n)

Ar-Cl, heat or Pd catalyst

Example 45

1,5-Anhydro-2,3-dideoxy-3-{[(1 R,3S)-3-(1-hydroxycyclobutyl)-3-({(1 S,4S)-5-[6(trifluoromethyl )pyridin-2-yl]-2,5-diazabicyclo[2.2.1]hept-2yl}carbonyl)cyclopentyl]amino}-4-O-methyl-D-erythro-pentitol

Step 1. Benzyl (4Sl·4-(2,5-dimethvl-1H-pvrrol-1-vl)cvclopent-2-ene-1carboxylate

A solution of 11.04 g (50.4 mmol) of methyl (1R,4S)-4-(2,5-dimethyl-1H-pyrrol-1yl)cyclopent-2-ene-1-carboxylate and 40 mL of 2.5 M sodium hydroxide in 100 mL of MeOH was stirred at ambient température for 45 minutes, then concentrated under reduced pressure to remove methanol. The residual aqueous solution was added to a stirred mixture of 20 g citric acid, 100 mL of water, and 100 mL of dichloromethane. The phases were separated and the aqueous extracted with two additional portions of dichloromethane. The organic phase was dried (Na₂SO4) and concentrated under reduced pressure to provide the correponding acid as a brown gum (10.98 g).

To a stirred solution of this acid, 10 mL (96 mmol) of benzyl alcohol, and 1.74 g (14 mmol) of DMAP in 50 mL of dichloromethane was added 13 g (69 mmol) of EDC, in five equal portions over 30 min. After 18 h, the solution was concentrated under reduced pressure, and the residual syrup partitioned between ether and water. The organic phase was washed with water and brine, with one back-extraction of the aqueous phases with ether. The combined organic phase was dried (MgSO4), and concentrated under reduced pressure. Flash chromatography of the crude material on silica using 10% ethyl acetate in heptane afforded 13.75 g (93%) of the title compound as a colorless oiL 1H NMR (400 MHz, CHLOROFORM-d) showed a 60:40 mixture of epimers: δ ppm 2.09 2.32 (m, 1 H) 2.20 (s, 6 H) 2.67 - 2.85 (m, 1 H) 3.62 - 3.70 (m, 0.6 H) 3.85 - 3.92 (m, 0.4 H) 5.16 (2s, 2 H) 5.26 - 5.34 (m, 0.6 H) 5.49 - 5.56 (m, 0.4 H) 5.74 (br. s.,

Η) 5.93 - 6.04 (m, 2 Η) 7.30 - 7.42 (m, 5 Η). TLC Rf 0.32 and 0.25 (10% ethyl acetate in hexane). LC-MS ES+ 296.2.

Step 2. Benzyl (1 R,4S )-4-(2,5-dimethvl-1H-pyrrol-1-vl)-1-(1hvdroxvcvclobutvl)cvclopent-2-ene-1-carboxylate

To a cold (-78 C), stirred solution of 6.17 g (20.9 mmol) of benzyl (4S)-4-(2,5dimethyl-1H-pyrrol-1-yl)cyclopent-2-ene-1-carboxylate (Préparation SRT-0233) in 40 mL of dry THF, under Ar, was slowly added 23.3 mL of a commercial 1.8 M solution of LDA. The resulting solution was warmed to 0 C, stirreed at that température for 10 min, and then recooled to -78 C for the addition of 2.3 mL (31 mmol) of cyclobutanone. This solution was stirred at -78 C for 1 h, then quenched at -78 C by the slow addition of a solution of 4.0 mL of 12N HCl in 10 mL of THF. Ethyl acetate and excess 1 M citric acid were added and the mixture was allowed to warm to RT. Extractive workup was followed by flash chromatography on silica using 25% ethyl acetate in heptane to provide 5.55 g (73%) of the title compound as a thick amber oil. ¹H NMR (400 MHz, CHLOROFORM-d) δ ppm 1.44 -1.56 (m, 1 H) 1.88 - 2.31 (m, 6 H) 2.12 (s, 6 H) 2.27 (dd, >14.7, 7.5 Hz, 1 H) 2.76 (dd, >14.5, 9.0 Hz, 1 H) 5.18 (s, 2 H) 5.26 - 5.32 (m, 1 H) 5.71 (s, 2 H) 6.06 (dd, 1 H) 6.10 (dd, 1 H) 7.30 - 7.39 (m, 5 H). TLC Rf 0.36 (30% ethyl acetate in hexane). LC-MS ES+ 366.2.

Step 3· Benzvl (1R.4S)-4-amino-1-(1“hydroxvcvclobutvl)cvclopent-2-ene-1carboxylate

A mixture of 5.52 g (15.1 mmol) of benzyl (1R,4S)-4-(2,5-dimethyl-1H-pyrrol-1yl)-1 -(1 -hydroxycyclobutyl)cyclopent-2-ene-1 -carboxylate (Préparation SRT0229), 8.20 g (120 mmol) of hydroxylamine hydrochloride, and 7.0 mL of 50% aqueous hydroxylamine (100 mmol) in 50 mL of methanol was heated at 68 C for 8 h, then cooled. Sufficient water was added to dissolve the crystals which deposited, and the solution was concentrated under reduced pressure to remove methanol. The resulting mixture was brought to pH ~10 with aq. NaOH and then extracted with several portions of dichloromethane. The organic phase was dried over Na₂SO4 and then concentrated under reduced pressure to afford 4.15 g (96%) of the title amine as a nearly colorless oil, of sufficient purity for the subséquent reaction. ¹H NMR (400 MHz, CHLOROFORM-d) δ ppm 1.42 -1.53 (m, 1 H)1.85(dd, 1 H) 1.88 - 2.08 (m, 4 H) 2.19 - 2.27 (m, 1 H) 2.46 (dd, J=14.5, 8.3 Hz, 1 H) 3.97 - 4.02 (m, 1 H) 4.70 (s, 1 H) 5.16 (s, 2 H) 5.84 (dd, J=5.6, 1.6 Hz, 1 H) 5.98 (dd, J=5.6, 2.1 Hz, 1 H) 7.31 - 7.38 (m, 5 H). LC-MS ES+ 288.2.

Step 4. 1.5-Anhvdro-3-f[(1S,4R)-4-[(benzvloxv)carbonvn-4-(1hvdroxvcvclobutvl)cvclopent-2-en-1-vllaminoΐ-2_l3-dideoxv-4-O-methvl·D-ervthropentitol

OMe

ιοο

A solution of 3.83 g (13.3 mmol)of benzyl (1R,4S)-4-amino-1-(1hydroxycyclobutyl)cyclopent-2-ene-1-carboxylate (Préparation SRT-0235) and 1.89 g (14.5 mmol) of (3R)-3-methoxytetrahydro-4H-pyran-4-one in 26 mL of dichloromethane was stirred with activated 3A molecular sieves for 10 min, then cooled to 0 C. Sodium triacetoxyborohydride (3.35 g, 15.8 mmol) was added in several portions over 10 min, and the mixture was stirred for 1.5 h. The cloudy mixture was added to dichloromethane and aqueous NaHCO₃ + NaOH (pH —14), and the aqueous phase was extracted with several additional portions of dichloromethane. The organic phase was dried (Na₂SO₄) and concentrated under reduced pressure. Flash chromatography of the residue on silica using 35% methanolic ammonia (7M) in dichloromethane afforded 3.94 g (74%) of the title compound as a yellow oil. ¹H NMR (400 MHz, CHLOROFORM-d) δ ppm 1.41-1.52 (m, 1 H) 1.55 - 1.64 (m, 1 H) 1.64 - 1.79 (m, 2 H) 1.86 - 2.09 (m, 5 H) 2.19 - 2.29 (m, 1 H) 2.41 (dd, J=14.2, 7.7 Hz, 1 H) 2.84 - 2.91 (m, 1 H) 3.17 3.44 (m, 4 H) 3.34 (s, 3 H) 3.88 - 3.96 (m, 2 H) 4.00 (dd, J=12.3, 4.4 Hz, 1 H) 5.10-5.20 (m, 2 H) 5.88 - 5.93 (m, 1 H) 6.00 (dd, J=5.6, 1.9 Hz, 1 H) 7.28-7.39 (m, 5 H). TLC Rf 0.41 (4% 7M methanolic ammonia in dichloromethane). LC-MS ES+ 402.1.

Step 5. 1,5-Anhvdro-3-ffî1S,4R)-4-Kbenzvloxv)carbonyll-4-(1hydroxycyclobutyl)cvclopent-2-en-1-vl1(tnfluoroacetvl)amino)-2,3-dideoxv-4-Qmethyl-D-erythro-pentitol

To a cold (0 C), stirred solution of 805 mg (2.00 mmol) of 1,5-anhydro-3{[(1S,4R)-4-[(benzyloxy)carbonyl]-4-(1-hydroxycyclobutyl)cyclopent-2-en-1yl]amino}-2,3-dideoxy-4-O-methyl-D-erythro-pentitol and 0.87 mL (5.0 mmol) of diisopropylethylamîne in 6 mL of dichloromethane was added dropwise 0.61 mL (4.4 mmol) of trifluoroacetic anhydride. The solution was stirred for 2 h, then

ΙΟΙ stirred with aqueous sodium citrate for 30 min. The organic phase was separated, dried (Na₂SO₄), and concentrated under reduced pressure to 1.36 g of brown oil. This was dissolved in 5 mL of methanol and treated with 2 mL of 7M methanolic ammonia. After 1 h, the solution was concentrated under reduced pressure, and the residue chromatographed on silica using 15-20% ethyl acetate in dichloromethane to provide 1.11 g (111%) of the title compound as an amber oil, still retaining some solvent. ¹H NMR (400 MHz, CHLOROFORM-d) δ ppm 1.37-1.49 (m, 1 H) 1.50 - 1.57 (m, 1 H) 1.78 - 2.00 (m, 3 H) 2.01 -2.10(m, 1 H) 2.26 - 2.38 (m, 3 H) 2.40 - 2.53 (m, 1 H) 3.10 (br. s., 0 H) 3.31 (d, J=13.0 Hz, 1 H) 3.39 - 3.45 (m, 1 H) 3.43 (s, 3 H) 3.50 (t, J=11.4 Hz, 1 H) 3.89 (d, J=11.6 Hz, 1 H) 4.06 - 4.14 (m, 1 H) 4.19 (d, J=13.0 Hz, 1 H) 5.00 (t, 1 H) 5.14 (d, J=12.3 Hz, 1 H) 5.30 (d, J=12.3 Hz, 1 H) 5.78 (dd, J=5.8, 2.0 Hz, 1 H) 6.12 (dd, J=5.8, 2.0 Hz, 1 H) 6.57 (br. s., 1 H) 7.28 - 7.42 (m, 5 H). TLC Rf 0.34 (25% ethyl acetate in dichloromethane). LC-MS ES+ 498.2.

Step 6. 3-rf(1S.4R)-4-[1-(Acetvloxv)cvclobutvl1-4-Rbenzvloxv)carbonvncvclopent2-en-1-vlÎ(trifluoroacetvl)aminol-1_l5-anhvdro-2,3-dideoxv-4-O-methvl-D-erythropentitol

To a stirred solution of 3.58 g (7.2 mmol) of 1,5-anhydro-3-{[(1S,4R)-4[(benzyloxy)carbonyl]-4-( 1 -hydroxycyclobutyl)cyclopent-2-en-1 yl](trifluoroacetyl)amino}-2,3-dideoxy-4-O-methyl-D-erythro-pentitol, 1.6 mL (9.2 mmol) of diisopropylethylamine, and 100 mg (0.82 mmol) of DMAP in 10 mL of dichloromethane was added dropwise 5.1 mL (72 mmol) of acetyl chloride. The solution was stirred at ambient température for 18 h, then cooled in ice and quenched with aqueous NaHCO₃. The aqueous phase was extracted with additional dichloromethane, and the combined organic phases dried (Na₂SO₄)

102 and concentrated under reduced pressure. Flash chromatography of the residue on silica using 8-10% ethyl acetate in dichloromethane provided 2.63 g (68%) of the title compound as a viscous yellow oil. ¹H NMR (400 MHz, CHLOROFORMd) δ ppm 1.35-1.48 (m, 1 H) 1.54-1.61 (m, 1 H) 1.82 - 1.94 (m, 1 H) 1.88 (s, 3 H) 2.17 - 2.26 (m, 1 H) 2.34 (dd, J=14.7, 9.9 Hz, 1 H) 2.43 - 2.64 (m, 5 H) 3.32 (d, J=12.6 Hz, 1 H) 3.37 - 3.43 (m, 1 H) 3.43 (s, 3 H) 3.46 - 3.55 (m, 1 H) 3.84 3.94 (m, 1 H) 4.09 - 4.15 (m, 1 H) 4.19 (d, J=13.0 Hz, 1 H) 4.98 - 5.05 (m, 1 H) 5.08 (d, J=12.3 Hz, 1 H) 5.23 (d, J=12.3 Hz, 1 H) 5.75 (dd, J=5.8, 2.0 Hz, 1 H) 6.16 (dd, J=5.8, 2.4 Hz, 1 H) 7.28 - 7.43 (m, 5 H). TLC Rf 0.39 (10% ethyl acetate-dichloromethane). LC-MS ES+ 540.2.

Step 7. 3-[f(1 R,3S)-3-(1-(Acetyloxv)cvclobutyl1-3carboxvcvclopentvl}(tnfluoroacetvnamÎno1-1,5-anhvdro-2.3-dideoxv-4-O-methvlD-erythro-pentitol

A mixture of 2.62 g (4.86 mmol) of 3-[{(1S,4R)-4-[1-(acetyloxy)cyclobutyl]-4[(benzyloxy)carbonyl]cyclopent-2-en-1-yl}(trifluoroacetyl)amino]-1,5-anhydro-2,3dideoxy-4-O-methyl-D-erythro-pentitol and 750 mg each of 5% Pt/C and 5% Pd/C in 10 mL of acetic acid was stirred rapidly under 50 psi hydrogen gas for 18 h, then filtered through diatomaceous earth to remove catalyst. The filtrate was concentrated under reduced pressure, with added toluene to azeotropically remove acetic acid, to provide 2.25 g (103%) of the title compound as a white foam. ¹H NMR (400 MHz, CHLOROFORM-d) δ ppm 1.50 -1.71 (m, 3 H) 1.741.84 (m, 1 H) 2.02 (s, 3 H) 2.04 - 2.17 (m, 1 H) 2.34 - 2.55 (m, 5 H) 2.60 (dd, J=14.3, 8.5 Hz, 1 H) 2.66 - 2.81 (m, 2 H) 3.30 (d, J=13.0 Hz, 1 H) 3.35 - 3.39 (m, 1 H) 3.42 (s, 3 H) 3.47 - 3.56 (m, 1 H) 3.83 - 3.91 (m, 1 H) 4.13 (dd, J=11.6, 4.4 Hz, 1 H) 4.16-4.23 (m, 1 H) 4.26-4.38 (m, 1 H). LC-MS ES+ 452.1.

103

Step 8. 3-{i(1R.3S)-341-(Acetvloxv)cvclobutvl1-3-ffî1S,4S)-5-(tertbutoxvcarbonvl)-2,5-diazabicvclo[2.2.11hept-2vllcarbonvlÎcyclopentvl1(trifluoroacetvlÎaminoM.5-anhvdro-2,3-dideoxy-4-Qmethvl-D-erythro-pentitol

A stirred solution of 1.42 g (3.15 mmol) of 3-[{( 1 R,3S)-3-[1 (acetyloxy)cyclobutyl]-3-carboxycyclopentyl}(trifluoroacetyl)amino]-1,5-anhydro2,3-dideoxy-4-O-methyl-D-erythro-pentitol in 6 mL of dichloromethane was treated under argon with 6.5 mL of a 2M dichloromethane solution of oxalyl chloride and 2 drops of dry DMF. After 2 h, the solution was taken to dryness under reduced pressure to give the acid chloride as a yellow foam.

This material was dissolved in 8 mL of dichloromethane. To 6 mL of the stirred solution was added 562 mg (2.84 mmol) of (1S,4S)-(-)-2-Boc-2,5diazabicyclo[2.2.1]heptane and 1.2 mL (6.9 mmol) of diisopropylethylamine. The reaction mixture was stirred at ambient température for 18 h, then partitioned between dichloromethane and 1M aqueous citric acid. The organic phase was dried (Na₂SO₄) and concentrated under reduced pressure. Flash chromatography of the residue on silica using 40-50% ethyl acetate in dichloromethane afforded 1.44 g (97%) of the title compound as a whitefoam. ¹H NMR (400 MHz, CHLOROFORM-d) Ô ppm 1.34 - 1.62 (m, 12 H) 1.73 - 1.88 (m, 2 H) 1.88 - 2.27 (m, 7 H) 2.29 - 2.41 (m, 1 H) 2.40 - 2.65 (m, 4 H) 2.66 - 2.80 (m, 1 H) 3.27 - 3.64 (m, 9 H) 3.81 - 3.89 (m, 1 H) 4.08 - 4.24 (m, 2 H) 4.37 - 4.60 (m, 2 H) 4.85 - 4.94 (m, 1 H). TLC Rf 0.36 (1:1 ethyl acetate in dichloromethane). LCMS ES+ 632.3.

104

Step 9. 1,5-Anhvdro-3-ïï(1R.3S)-3-ffî1S,4S)-5-(tert-butoxycarbonvl)-2.5diazabicvclof2.2.1lhept-2-vncarbonylÎ-3-(1hvdroxvcvclobutvl)cvclopentvl1(trifluoroacetvl)amino)-2.3-dideoxv-4-O-methvl-D erythro-pentitol

A mixture of 1.43 g (2.26 mmol) of 3-{[(1R,3S)-3-[1-(acetyloxy)cyclobutyl]-3{[(1S,4S)-5-(tert-butoxycarbonyl)-2,5-diazabicyclo[2.2.1]hept-210 yl]carbonyl}cyclopentyl](trifluoroacetyl)amino}-1,5-anhydro-2,3-dideoxy-4-Omethyl-D-erythro-pentitol and 1.60 g (11.6 mmol) of powdered potassium carbonate in 8 mL of methanol was stirred at ambient température for 18 h, then partitioned between dichloromethane and brine, with sufficient water to dissolve solids. The organic phase was dried (Na₂SO₄) and concentrated under reduced pressure. Flash chromatography of the residue on silica using acetate to provide 1.33 g (100%) of the title compound as a whitefoam. ¹H NMR (400 MHz, CHLOROFORM-d) δ ppm 1.34-1.62 (m, 10 H) 1.66-2.93 (m, 15 H) 3.20 - 3.63 (m, 8 H) 3.66 - 4.03 (m, 3 H) 4.06 - 4.24 (m, 3 H) 4.25 - 4.65 (m, 2 H) 4.68 - 5.29 (m, 1 H). TLC Rf 0.33 (EtOAc). LC-MS ES+ 590.3.

Step 10. 1,5-Anhvdro-3-(r(1R.3S)-3-{[(1S,4S)-5-(tert-butoxvcarbonvl)-2.5diazabicyclor2.2.11heDt-2“Vllcarbonvn-3-(1-hvdroxvcvclobutvl)cvclopentvHaminol· 2,3-dideoxy-4-O-methvl-D-ervthro-pentitol o

O ¹

105

A mixture of 1.31 g (2.23 mmol) of 1,5-anhydro-3-{[(1R,3S)-3-{[(1S,4S)-5-(tertbutoxycarbonyl)-2,5-diazabicyclo[2.2.1]hept-2-yl]carbonyl}-3-(1hydroxycyclobutyl)cyclopentyl](trifluoroacetyl)amino}-2,3-dideoxy-4-O-methyl-Derythro-pentitol and 860 mg (23 mmol) of sodium borohydride in 6.3 mL of éthanol was stirred at ambient température for 18 h, then partitioned between water and dichloromethane. The aqueous phase was extracted with additional dichloromethane, and the combined organic phase dried (NazSOi) and concentrated under reduced pressure. Flash chromatography on silica using 615% methanol in dichloromethane afforded 1.08 g (98%) of the title compound as 10 a white foam. ¹H NMR (400 MHz, CHLOROFORM-d) δ ppm 1.42 -1.47 (m, 9 H)

1.56 - 2.35 (m, 14 H) 2.72 (br. s., 1 H) 3.11 - 3.68 (m, 10 H) 3.90 - 3.98 (m, 1 H) 4.08 (dd, J=12.6, 2.7 Hz, 1 H) 4.38 - 4.58 (m, 1 H) 4.84 - 5,16 (m, 1 H). LC-MS ES+ 494.3. HRMS calculated for C26H43N3O6: 494.3230; found: 494.3260.

Step 11. 1,5-Anhvdro-2,3-dideoxv-3-ff(1R,3S)-3-f(1S,4S)-2.5diazabicvclor2.2.1lhept-2-vlcarbonvll-3-(1-hydroxvcvclobutyl)cvclopentvllaminoÎ4-O-methvl-D-ervthro-pentitol dihydrochloride

Ό H O

To 902 mg (1.827 mmol) of 1,5-anhydro-3-{[(1R,3S)-3-{[(1S,4S)-5-(tertbutoxycarbonyl)-2,5-diazabicyclo[2.2.1]hept-2-yl]carbonyl}-3-(1hydroxycyclobutyl)cyclopentyl]amino}-2,3-dideoxy-4-O-methyl-D-erythro-pentitol was added 10 mL of 4M HCl in dioxane, and a few drops of methanol sufficient to 25 give a clear solution. The solution was stirred at ambient température for 45 minutes, then concentrated under reduced pressure to give 973 mg of the intermediate sait as a foam. ¹H NMR (400 MHz, MeOD) δ ppm 1.57 -1.70 (m, 1 H) 1.73- 1.93 (m, 4 H) 1.93 - 2.10 (m, 4 H) 2.14 - 2.28 (m, 2 H) 2.29 - 2.44 (m, 3

106

H) 2.46 - 2.59 (m, 2 H) 3.35 - 3.77 (m, 12 H) 3.98 (dd, J=11.6, 4.4 Hz, 1 H) 4.26 (d, J=13.3 Hz, 1 H) 4.45 (s, 1 H) 5.21 (br. s„ 1 H). LC-MS ES+ 394.2.

Example 45

1,5-Anhydro-2,3-dideoxy-3-{[(1R,3S)-3-(1-hydroxycyclobutyl)-3-({(1S,4S)-5-[6(trifluoromethyl)pyridin“2-yl]-2,5-diazabicyclo[2.2.1]hept-2yl}carbonyl)cyclopentyl]amino}-4-O-methyl-D-erythro-pentitol

A solution of 93 mg (0.20 mmol) of 1,5-anhydro-2,3-dideoxy-3-{[(1R,3S)-3[(1S,4S)-2,5-diazabicyclo[2.2.1]hept-2-ylcarbonyl]-3-(1hydroxycyclobutyl)cyclopentyl]amino}-4-O-methyl-D-erythro-pentitol dihydrochloride, 91 mg (0.49 mmol) of 2-chloro-6-trifluoromethylpyridine, and 0.14 mL (0.80 mmol) of diisopropylethylamine in 0.5 mL of DMSO was heated at 80 C for 18 h, then cooled and partitioned between ethyl acetate and water. The organic phase was dried (Na₂SO4) and concentrated under reduced pressure. The crude material was purified by préparative RP-HPLC using as the mobile phase acetonitrile-water containing 0.05% TFA. ¹H NMR (400 MHz, CHLOROFORM-d) δ ppm 1.46 - 2.52 (m, 16 H) 3.26 - 3.54 (m, 8 H) 3.53 - 3.92 (m, 4 H) 4.02 (dd, J=11.8, 4.3 Hz, 1 H) 4.22 (d, J=13.0 Hz, 1 H) 5.01 (d, J=14.3 Hz, 2 H) 6.47 (d, J=8.5 Hz, 1 H) 6.93 (d, J=7.5 Hz, 1 H) 7.56 (t, J=8.0 Hz, 1 H). HRMS calculated for C27H37N4O4F3: 539.2845; found: 539.2894.

107

Example 46

1,5-Anhydro-2,3-dideoxy-3-{[(1R,3S)-3-(1-hydroxycyclobutyl)-3-({(1S,4S)-5-[4(trifluoromethyl)pyridin-2-yl]-2,5-diazabicyclo[2.2.1]hept-2yl}carbonyl)cyclopentyl]amino}-4-O-methyl-D-erythro-pentitol

The title compound was prepared in a fashion analogous to that described in Préparation Ex. 45. ¹H NMR (400 MHz, CHLOROFORM-d) δ ppm 1.57 - 2.31 (m, 14 H) 2.33 - 2.47 (m, 2 H) 2.49 - 2.62 (m, 1 H) 3.28 - 3.54 (m, 7 H) 3.57 - 3.91 (m, 4 H) 4.02 (dd, >11.8, 4.3 Hz, 1 H) 4.22 (d, >13.3 Hz, 1 H) 5.07 (br. s., 1 H) 5.25 (s, 1 H) 6.88 - 6.96 (m, 2 H) 8.14 (d, >6.5 Hz, 1 H). HRMS calculated for C27H37N4O4F3: 539.2845; found: 539.2894.

Example 47

1,5-Anhydro-2,3-dideoxy-3-{[(1R,3S)-3-(1-hydroxycyclobutyl)-3-({(1S,4S)-5-[4(trifluoromethyl)pyrimidin-2-yl]-2,5-diazabicyclo[2.2.1]hept-2yl}carbonyl)cyclopentyl]amino}-4-O-methyl-D-erythro-pentitol

108

The tîtle compound was prepared in a fashion analogous to that described in Préparation Example 45. ¹H NMR (400 MHz, CHLOROFORM-d) δ ppm 1.65 2.51 (m, 15 H) 2.52 - 2.64 (m, 1 H) 3.35 (s, 3 H) 3.44 (d, J=13.7 Hz, 1 H) 3.49 3.98 (m, 7 H) 4.19 (dd, J=11.4, 4.6 Hz, 1 H) 4.35 (d, J=14.0 Hz, 1 H) 5.07-5.48 (m, 2 H) 7.16 (d, J=6.5 Hz, 1 H) 8.49 (d, J=6.5 Hz, 1 H). HRMS calculated for C26H36N5O4F3: 540.2797; found: 540.2812.

Example 48 'O O

1,5-Anhydro-2,3-dideoxy-3-{[(1R,3S)-3-(1-hydroxycyclobutyl)-3-({(1S,4S)-5-[2(trifluoromethyl)pyrimidin-4-yl]-2,5-diazabicyclo[2.2.1]hept-2yl}carbonyl)cyclopentyl]amino}-4-O-methyl-D-erythro-pentitol

The title compound was prepared in a fashion analogous to that described in Préparation Example 45. ¹H NMR (400 MHz, CHLOROFORM-d) δ ppm 1.57 2.53 (m, 16 H) 3.31 (d, J=13.3 Hz, 1 H) 3.35 - 3.79 (m, 10 H) 4.03 (dd, J=11.4, 4.3 Hz, 1 H) 4.23 (d, J=12.6 Hz, 1 H) 5.11 (s, 1 H) 5.29 (br. s., 1 H) 6.23 - 6.75 (m, 2 H) 8.30 (d, J=6.1 Hz, 1 H). HRMS calculated for C26H36N5O4F3: 540.2797; found: 540.2891.

109

Example 49

1,5- anhydro-2,3-dideoxy-3-{[(1 R,3S )-3-(1-hydroxycyclobutyl )-3-( {(1 S,4S)-5-[6(trifluoromethyl)pyrimidin-4-yl]-2,5-diazabicyclo[2.2.1]hept-2yl}carbonyl)cyclopentyl]amino}-4-O-methyl-D-erythro-pentitol

The title compound was prepared in a fashion analogous to that described in Préparation Example 45.¹H NMR (400 MHz, CHLOROFORM-d) δ ppm 1.56 2.35 (m, 14 H) 2.36 - 2.44 (m, 1 H) 2.45 - 2.54 (m, 1 H) 3.32 (d, J=13.3 Hz, 1 H) 3.36-3.92 (m, 10 H)4.04(dd, J=12.3, 4.1 Hz, 1 H) 4.24 (d, J=13.0 Hz, 1 H) 5.15 (s, 1 H) 5.23 - 5.30 (m, 1 H) 6.45 - 7.05 (m, 3 H) 8.67 (s, 1 H). HRMS calculated for C26H36N5O4F3: 540.2797; found: 540.2830.

Example 50

1,5-Anhydro-2,3-dideoxy-3-{[(1R,3S)-3-(1-hydroxycyclobutyl)-3-({(1S,4S)-5-[6(trifluoromethyl)pyrazin-2-yl]-2,5-diazabicyclo[2.2.1]hept-2yl}carbonyl)cyclopentyl]amino}-4-O-methyl-D-erythro-pentitol

HO

A solution of 93 mg (0.20 mmol) of 1,5-anhydro-2,3-dideoxy-3-{[(1R,3S)-3[(1 S,4S)-2,5-diazabicyclo[2.2.1]hept-2-ylcarbonyl]-3-(1hydroxycyclobutyl)cyclopentyl]amino}-4-O-methyl-D-erythro-pentitol dihydrochloride 121 mg (0.442 mmol) of 2-iodo-6-trifluoromethylpyrazine, 326 mg (1.0 mmol) of césium carbonate, 24 mg (40 umol) of XantPhos, and 16 mg (28 umol) of tris(dibenzylideneacetone) palladium (0) in 0.8 mL of dioxane and 0.4 mL of DMSO was heated at 80 C under argon for 18 h, then cooled and partitioned between ethyl acetate and water. The organic phase was dried (Na₂SO₄) and concentrated under reduced pressure. Flash chromatography of the residue on silica using 0-2-4% methanolic ammonia and 3% methanol in dichloromethane afforded 89 mg (82%) of the title compound. ¹H NMR (400 MHz, CHLOROFORM-d) δ ppm 1.51 - 2.40 (m, 16 H) 2.65 - 2.74 (m, 1 H) 3.09 - 3.49 (m, 10 H) 3.54 (d, J=9.6 Hz, 1 H) 3.61 - 3.80 (m, 2 H) 3.88 - 3.96 (m, 1 H) 4.06 (dd, J=12.5, 2.9 Hz, 1 H) 4.96 (br. s„ 1 H) 5.21 (br. s., 1 H) 8.02 (s, 1 H) 8.15 (s, 1 H). HRMS calculated for C26H36N5O4F3: 540.2797; found: 540.2896.

Example 51

1,5-Anhydro-2,3-dideoxy-3-{[(1R,3S)-3-(1-hydroxycyclobutyl)-3-({(1S,4S)-5-[2(trifluoromethyl)pyridin-4-yl]-2,5-diazabicyclo[2.2.1]hept-2yl}carbonyl)cyclopentyl]amino}-4-O-methyl-D-erythro-pentitol

The title compound was prepared in a fashion analogous to that described in Example 45.¹H NMR (400 MHz, CHLOROFORM-d) δ ppm 1.51 - 2.40 (m, 18 H) 2.65 - 2.73 (m, 1 H) 3.04 - 3.47 (m, 8 H) 3.59 (d, J=8.5 Hz, 1 H) 3.70 (d, J=9.9 Hz, 1 H) 3.86 - 3.95 (m, 1 H) 4.05 (dd, J=12.3, 2.4 Hz, 1 H) 4.52 (s, 1 H) 5.17 (br.

s., 1 H) 6.48 (br. s., 1 H) 6.72 (br. s., 1 H) 8.26 (d, J=5.8 Hz, 1 H). HRMS calculated for C27H37N4O4F3: 539.2845; found: 539.2979.

Example 52

1,5-Anhydro-2,3-dideoxy-3-{[3-(1-hydroxy-1-methylethyl)-3-({5-[4(trifluoromethyl)pyridin-2-yl]-2,5-diazabicyclo[2.2.1]hept-2yl}carbonyl)cyclopentyl]amino}4“O-methylpentitol

Step 1. Benzyl 4-(2_l5-dimethvl-1H-pyrrol-1-vn-1-(1-hvdroxv-1methylethyl)cyclopent-2-ene-1-carboxvlate

The title compound was prepared in a manner analogous to that described in Example 45, Step 2 above. ¹H NMR (400 MHz, CHLOROFORM-d) δ ppm 1.18 (s, 3 H) 1.22 (s, 3 H) 2.11 (s, 6 H) 2.22 (dd, J=14.7, 7.5 Hz, 1 H) 2.88 (dd.

J=14.7, 8.5 Hz, 1 H) 3.43 (s, 1 H) 5.15 - 5.23 (m, 2 H) 5.24 - 5.31 (m, 1 H) 5.71 (s, 2 H) 5.99 (dd, 1 H) 6.03 (dd, 1 H) 7.30 - 7.40 (m, 5 H). TLC Rf 0.29 (30% ethyl acetate in hexane). LC-MS ES+ 354.2.

112

Step 2 Benzyl 4-amino-1-(1-hvdroxv-1-methvlethyl)cyclopent-2-ene-1carboxylate o

The title compound was prepared in a manner analogous to that described in Example 45, Step 3 above. ¹H NMR (400 MHz, CHLOROFORM-d) δ ppm 1.11 (s, 3 H) 1.14 (s, 3 H) 1.81 (dd, J=14.5, 4.9 Hz, 1 H) 1.85 -1.92 (m, 1 H)2.67(dd, J=14.3, 8.2 Hz, 1 H) 3.97 - 4.03 (m, 1 H) 5.18 (s, 2 H) 5.83 (dd, 1 H) 5.89 (dd, 1 H) 7.32 - 7.42 (m, 5 H). LC-MS ES+ 276.2.

Step 3 1.5-Anhvdro-3-(i4T(benzvloxvÎcarbonvll-4-(1-hydroxv-1methvlethvl)cvclopent-2-en-1-ylÎamino)-2,3-dideoxv-4-O-methylpentitol

The title compound was prepared in a manner analogous to that described in Example 45,step 4 above. ¹H NMR (400 MHz, CHLOROFORM-d) δ ppm 1.11 (s, 3 H) 1.15 (s, 3 H) 1.41 - 1.66 (m, 2 H) 1.68-1.80 (m, 1 H) 1.87-1.94 (m, 1 H) 2.56 - 2.65 (m, 1 H) 2.82 - 2.89 (m, 1 H) 3.24 - 3.46 (m, 7 H) 3.87 - 3.97 (m, 2 H) 3.98 - 4.07 (m, 1 H) 5.11 - 5.21 (m, 2 H) 5.84 - 5.97 (m, 2 H) 7.30 - 7.41 (m, 5 H). TLC Rf 0.34 (4% methanolic ammonia in dichloromethane). LC-MS ES+ 390.2.

H3

Step 4. 1,5-Anhydro-3-[{44(benzvloxv)carbonyl1-4-( 1 -hydroxy-1 methvlethvDcvciooent-2-en-1-vlktrifluoroacetvl)amino1-2.3-dideoxv-4-Qmethylpentitol

The title compound was prepared in a manner analogous to that described in example 45, step 5 above. ¹H NMR (400 MHz, CHLOROFORM-d) δ ppm 1.35 1.75 (m, 2 H) 1.78 - 2.08 (m, 4 H) 2.24 - 2.54 (m, 4 H) 3.31 (d, J=12.6 Hz, 1 H) 3.38 - 3.45 (m, 2 H) 3.43 (s, 3 H) 3.45 - 3.55 (m, 1 H) 3.84 - 3.94 (m, 1 H) 4.07 4.14 (m, 1 H) 4.19 (d, J=13.0 Hz, 1 H) 4.95 - 5.05 (m, 1 H) 5.14 (d, J=12.3 Hz, 1 H) 5.31 (d, 1 H) 5.78 (dd, J=5.6, 2.2 Hz, 1 H) 6.12 (dd, J=5.8, 2.4 Hz, 1 H) 7.27 7.44 (m, 5 H). Rf-0.34 (25% ethyl acetate-dichloromethane). LC-MS ES+ 498.2.

Step 5. 3-K4-ri-(acetvloxv)-1-methvlethvll-4-r(benzvloxv)carbonvl1cvclopent-2en-1-vlÎ(trifluoroacetvl)amino1-1.5-anhydro-2_t3-dideoxy-4-O-methvipentitol

The title compound was prepared in a manner analogous to that described in example 45, step 6 above. ¹H NMR (400 MHz, CHLOROFORM-d) δ ppm 1.49 (s, 3 H) 1.53 -1.61 (m, 4 H) 1.82 - 1.86 (m, 3 H) 2.38 - 2.57 (m, 2 H) 3.32 (d, J=13.0 Hz, 1 H) 3.37 - 3.44 (m, 3 H) 3.45 - 3.56 (m, 2 H) 3.84 - 3.95 (m, 1 H) 4.05 - 4.23 (m, 2 H) 4.93 - 5.04 (m, 1 H) 5.08 - 5.15 (m, 1 H) 5.26 - 5.36 (m, 3 H) 6.11 (dd,

114

J=5.8, 2.4 Hz, 1 H) 7.28 - 7.42 (m, 5 H). TLC Rf 0.29 (40% ethyl acetatehexane). LC-MS ES+ 550.2 for M+Na.

Step 6. 3-K3-[1-(AcetvloxvM-methvlethyl1-3carboxvcvclopentvl}(trifluoroacetyl)amino1-1,5-anhydro-2,3-dideoxy-4-Omethylpentitol

The title compound was prepared in a manner analogous to that described in example 45, step 7 above. ¹H NMR (400 MHz, CHLOROFORM-d) Ô ppm 0.86 (none, 1 H) 1.51 -1.91 (m, 10 H) 2.02 - 2.04 (m, 3 H) 2.24 - 2.54 (m, 3 H) 3.31 (d, J=13.0 Hz, 1 H) 3.37 (br. s., 1 H) 3.41 - 3.46 (m, 3 H) 3.46 - 3.56 (m, 1 H) 3.82 - 3.91 (m, 1 H) 4.08 - 4.16 (m, 1 H) 4.16 - 4.23 (m, 1 H) 4.25 - 4.39 (m, 1 H). LC-MS ES+ 440.2.

Step 7. 3-K3-ri-(Acetvloxv)-1-methvlethyl1-3-n5-(tert-butoxvcarbonvl)-2.5diazabicvclo[2.2.1lhept-2-vllcarbonvl}cyclopentvl)(trifluoroacetvl)aminol-1.5anhvdro-2,3-dideoxy-4-O-methvlpentitoi

The title compound was prepared in a manner analogous to that described in example 45, step 8 above. ¹H NMR (400 MHz, CHLOROFORM-d) δ ppm 1.35 1.51 (m, 9 H) 1.54 -1.66 (m, 6 H) 1.72 -1.87 (m, 2 H) 1.97 - 2.33 (m, 7 H) 2.36 2.65 (m, 2 H) 3.25 - 3.68 (m, 9 H) 3.80 - 3.90 (m, 1 H) 4.06 - 4.25 (m, 2 H) 4.34 4.64 (m, 2 H) 4.88 - 4.99 (m, 1 H). TLC Rf 0.38. LC-MS ES+ 642.3 (M+Na).

Step 8. 1,5-Anhvdro-3-(r3-ir5-(tert-butoxvcarbonvl)-2.5-diazabicvclor2.2.1lheDt-2vl1carbonvl)-3-(1-hvdroxv-1-methvlethvl)cvclopentvl1(trifluoroacetvl)aminoÎ-2.3dideoxv-4-O-methvlpentitol

The title compound was prepared in a manner analogous to that described in exampie 45, Step 9 above. ¹H NMR (400 MHz, CHLOROFORM-d) δ ppm 1.08 1.28 (m, 5 H) 1.36 - 2.77 (m, 18 H) 3.25 - 3.57 (m, 8 H) 3.68 - 3.91 (m, 2 H) 4.06 - 4.24 (m, 2 H) 4.37 - 4.73 (m, 2 H) 4.82 - 5.00 (m, 1 H). TLC Rf 0.30 (2% MeOH in ethyl acetate). LC-MS ES+ 578.3.

Step 9. 1,5-anhvdro-3-([3-{f5-(tert-butoxvcarbonvl)-2,5-diazabicvclo[2.2.11hept-2vl1carbonvl}-3-(1-hvdroxv-1-methvlethvl)cvclopentvnamino}-2.3-dideoxv-4-Om ethyl penti toi

116

The title compound was prepared in a manner analogous to that described in example 45, step 10.¹H NMR (400 MHz, CHLOROFORM-d) δ ppm 1.10-1.26 (m, 6 H) 1.37- 1.90 (m, 16 H) 2.23 - 2.52 (m, 2 H) 2.63 - 2.74 (m, 1 H) 3.103.72 (m, 10 H) 3.84 - 3.97 (m, 1 H) 4.03 - 4.12 (m, 1 H) 4.34 - 4.62 (m, 1 H) 4.80 -4.99 (m, 1 H). TLC Rf 0.19 (4% methanolic ammonia in dichloromethane). HRMS calculated for C25H43N3O6: 482.3230; found: 482.3276.

Step 10 1,5-Anhvdro-2,3-dideoxv-3-n3-(2,5-diazabicvclo(2.2.11hept-2ytcarbonyl )-3-(1-h vdroxv-1-methylethvl)cvclopentvllaminoÎ-4-O-methylpentitol

O 'OH

The title compound was prepared in a manner analogous to that described in example 45, step 11 above. ¹H NMR (400 MHz, CD3OD) δ ppm 1.16 (s, 3 H) 1.23 - 1.25 (m, 3 H) 1.62 - 2.34 (m, 7 H) 2.66 (br. s., 2 H) 3.33 - 3.68 (m, 14 H) 3.98 (dd, J=11.8, 4.6 Hz, 1 H) 4.26 (d, J=13.3 Hz, 1 H) 4.44 (s, 1 H). LC-MS ES+ 382.3.

Example 52

1,5-Anhydro-2,3-dideoxy-3-{[3-(1-hydroxy-1-methylethyl)-3-({5-[4(trifluoromethyl)pyridin-2-yl]-2,5-diazabicyclo[2.2.1]hept-2yl}carbonyl)cyclopentyl]amino}-4-O-methylpentitol

The title compound was prepared in a manner analogous to that described in Example 45. ¹H NMR (400 MHz, DMSO-d_e) δ ppm 1.00 - 1.20 (m, 6 H) 1.48 1.60 (m, 1 H) 1.65 - 2.47 (m, 8 H) 3.13 - 3.22 (m, 1 H) 3.23 - 3.54 (m, 8 H) 3.55 3.64 (m, 1 H) 3.80 - 3.90 (m, 1 H) 4.08 - 4.16 (m, 1 H) 4.87 - 5.02 (m, 1 H) 5.15 5.26 (m, 1 H) 6.84 (d, J=5.1 Hz, 1 H) 8.28 (d, J=5.5 Hz, 1 H). HRMS calculated for C26H37N4O4F3: 527.2845; found: 527.2939.

Example 53

1,5-Anhydro-2,3-dideoxy-3-{[3-(1-hydroxy-1-methylethyl)-3-({5-[6(trifluoromethyl)pyrimidin-4-yl]-2,5-diazabicyclo[2.2.1]hept-2yl}carbonyl)cyclopentyl]amino}-4-O-methylpentitol

The title compound was prepared in a manner analogous to that described in Example 45.¹H NMR (400 MHz, DMSO-d_e) δ ppm 0.92 -1.19 (m, 6 H) 1.48 1.62(m, 1 H) 1.64 - 2.47 (m, 8 H) 3.09 - 3.68 (m, 10 H) 3.81 - 3.91 (m, 1 H) 4.06 - 4.18 (m, 1 H) 4.87 - 5.04 (m, 1 H) 5.09 - 5.33 (m, 1 H) 8.29 - 8.64 (m, 2 H). HRMS calculated for C25H36N5O4F3: 528.2797; found: 528.2812.

Ils

Example 54 o

1,5-Anhydro-2,3-dideoxy-3-{[3-(1-hydroxy-1-methylethyl)-3-({5-[4(trifluoromethyl)pyrimidin-2-yl]-2,5-diazabicyclo[2.2.1]hept-2yl}carbonyl)cyclopentyl]amino}-4-O-methylpentitol

The title compound was prepared in a manner analogous to that described in Example 45.¹H NMR (400 MHz, DMSO-d₆) δ ppm 0.99 -1.20 (m, 6 H) 1.45 1.64 (m, 1 H) 1.63-2.18 (m, 7 H) 2.25 - 2.47 (m, 2 H) 3.13 - 3.65 (m, 10 H) 3.78 - 3.91 (m, 1 H) 4.12 (dd, 1 H) 4.87 - 5,04 (m, 2 H) 5.20 (br. s., 1 H) 8.67 (d, J=3.8 Hz, 1 H). HRMS calculated for C25H36N5O4F3: 528.2797; found: 528.2856.

Example 55

Ό O

1,5-Anhydro-2,3-dideoxy-3-{[3-(1-hydroxy-1-methylethyl)-3-({5-[5(trifluoromethyl)pyridazin-3-yl]-2,5-diazabicyclo[2.2.1]hept-2yl}carbonyl)cyclopentyl]amino}-4-O-methylpentitol

The title compound was prepared in a manner analogous to that described in Example 45.¹H NMR (400 MHz, DMSO-d₆) δ ppm 0.87 -1.20 (m, 7 H) 1.49

2.46 (m, 10 H) 3.16 - 3.73 (m, 10 H) 3.79 - 3.89 (m, 1 H) 4.08 - 4.17 (m, 1 H) 5.20 - 5.31 (m, 1 H) 8.30 - 8.61 (m, 2 H) 8.84 (s, 1 H). HRMS calculated for C25H36N5O4F3: 528.2797; found: 528.2863.

Example 56

1,5-Anhydro-2,3-dideoxy-3-{[3-(1-hydroxy-1-methylethyl)-3-({5-[2(trifluoromethyl)pyrimidin-4-yl]-2,5-diazabicyclo[2.2.1]hept-2yl}carbonyl)cyclopentyl]amino}-4-O-methylpentitol

The title compound was prepared in a manner analogous to that described in Example 45.¹H NMR (400 MHz, DMSO-d₆) δ ppm 0.96 -1.20 (m, 6 H) 1.45 2.48 (m, 9 H) 3.08 - 3.66 (m, 10 H) 3.80 - 3.89 (m, 1 H) 4.08 - 4.17 (m, 1 H) 4.85 - 5.11 (m, 1 H) 5.16 - 5.29 (m, 1 H) 8.27 - 8.34 (m, 1 H). HRMS calculated for C25H36N5O4F3: 528.2797; found: 528.2927.

Example 57

120

1,5-Anhydro-2,3-dideoxy-3-{[3-(1-hydroxy-1-methylethyl)-3-({5-[6(trifluoromethyl)pyridin-2-yl]-2_t5-diazabicyclo[2.2.1]hept-2yl}carbonyl)cyclopentyl]amino}-4-O-methylpentitol

The title compound was prepared in a manner analogous to that described in Example 45.¹H NMR (400 MHz, CHLOROFORM-d) δ ppm 0.82 - 1.37 (m, 6 H) 1.64-2.67 (m, 10 H) 3.19 - 3.78 (m, 11 H) 3.91 - 4.08 (m, 1 H) 4.15 - 4.28 (m, 1 H) 4.93 - 5.09 (m, 1 H) 6.40 - 6.54 (m, 1 H) 6.92 (d, J=7.2 Hz, 1 H) 7.55 (t, J=8.0 Hz, 1 H). HRMS calculated for C26H37N4O4F3: 527.2845; found: 527.2869.

Example 58

O

1,5-Anhydro-2,3-dideoxy-3-{[3-(1-hydroxy-1-methylethyl)-3-({5-[2(trifluoromethyl)pyridin-4-yl]-2,5-diazabicyclo[2.2.1]hept-2yl}carbonyl)cyclopentyl]amino}-4-O-methylpentitol

The title compound was prepared in a manner analogous to that described in Example 45.¹H NMR (400 MHz, DMSO-d_e) δ ppm 0.99 -1.19 (m, 6 H) 1.47 -

1.62 (m, 1 H) 1.64 - 2.46 (m, 9 H) 3.08 - 3.68 (m, 11 H) 3.80 - 3.91 (m, 1 H) 4.07 - 4.18 (m, 1 H) 4.75 - 5.28 (m, 2 H) 8.24 (d, J=5.8 Hz, 1 H) 8.30 - 8.77 (m, 2 H). HRMS calculated for C26H37N4O4F3: 527.2845; found: 527.2943.

I2l

Example 59 ο

1,5-Anhydro-2,3-dideoxy-3-{[3-(1 -hydroxy-1 -methylethyl)-3-({5-[6(trifluoromethyl)pyrazin-2-yl]-2,5-diazabicyclo[2.2.1]hept-2yl}carbonyl)cyclopentyl]amino}-4-O-methylpentitol

The title compound was prepared in a manner analogous to that described in Example 50.¹H NMR (400 MHz, DMSO-d₆) δ ppm 0.99 -1.19 (m, 6 H) 1.45 2.48 (m, 10 H) 3.15 - 3.69 (m, 10 H) 3.80 - 3.90 (m, 1 H) 4.07 - 4.17 (m, 1 H) 4.88 - 5.31 (m, 2 H) 8.20 (s, 1 H) 8.28 - 8.63 (m, 3 H). HRMS calculated for C25H36N5O4F3: 528.2797; found: 528.2905.

Example 60/61

((1S,3R)-3-((3S,4S)-3-methoxy-tetrahydro-2H-pyran-4-ylamino)-1(tetrahydrofuran-3-yl)cyclopentyl)((1S,4S)-5-(4-(trifluoromethyl)pyridin-2-yl)-2,5diaza-bicyclo[2.2.1]heptan-2-yl)methanone

122 ¹HNMR (400 MHz, CD3OD) δ Π8.20 (d, 1H), 7.18 (s, 1H), 6.97 (d, 1H), 5.11 (m, 2H), 4.24 (d, 1H), 3.38-3.99 (m, 17H), 2.87 (m, 1H), 2.65 (m, 1H), 2.48 (m, 1H), 1.90-2.39 (m, 8H), 1.81 (m, 2H), 1.65 (m, 1 H).

¹HNMR (400 MHz, CD₃OD) δ 8.20 (d, 1 H), 7.18 (s, 1 H), 6.97 (d, 1 H), 5.11 (m, 2H), 4.24 (d, 1H), 3.38-3.99 (m, 17H), 2.87 (m, 1H), 2.65 (m, 1H), 2.48 (m, 1H), 1.90-2.39 (m, 8H), 1.81 (m, 2H), 1.65 (m, 1 H).

Example 62/63

O

((1S,3R)-3-((3S,4S)-3-methoxy-tetrahydro-2H-pyran-4-ylamino)-1-(2,2,2-trifluoro1-hydroxyethyl)cycÎopentyl)((1S,4S)-5-(4-(trifluoromethyl)pyridin-2-yl)-2,5-diazabicyclo[2.2.1]heptan-2-yl)methanone ¹HNMR (400 MHz, CD3OD) ô 8.23 (d, J= 8Hz, 1H), 6.8 (d, J=8Hz, 1H), 6.72 (d,

J=8 Hz, 1 H), 5.0 (m, 1 H), 4.2, (m, 1 H), 3.85 (m, 2H), 3.6 (m, 1 H), 3.52 (m, 1 H),

3.30 (s, 3H) 3.4 (m, 4H), 2.7 (m, 2H), 2.4 (m,1H), 2.0 (m, 4H), 1.8 (m, 4H), 1.6 (m,4H)

1HNMR (400 MHz, CD3OD) δ 8.23 (d, J= 8Hz, 1H), 6.8 (d, J=8Hz, 1H), 6.72 (d,

J=8 Hz, 1H), 5.0 (m, 1H), 4.2, (m, 1H), 3.85 (m, 2H), 3.6 (m, 1H), 3.30 (s, 3H),

3.52 (m, 1H),3.4 (m, 4H), 2.7 (m, 2H), 2.4 (m,1H), 2.0 (m, 4H), 1.8 (m, 4H), 1.6 (m,4H)

123

Example 64

Ό Ο

((1S,3R)-3-((3S,4S)-3-methoxy-tetrahydro-2H-pyran-4-ylamino)-1-(2,2,2-trifluoro1-hydroxyethyl)cyclopentyl)((1S,4S)-5-(2-(trifluoromethyl)pyrimidin-4-yl)-2,5diaza-bicyclo[2.2.1]heptan-2-yl)methanone ¹HNMR (400 MHz, CD3OD) δ 8.23(m, 1 H), 6.6 (m, 1 H), 4.25 (m, 1 H), 4.0 (m, 2H), 3,85 (m, 1H), 3.7 (m, 1H) 3.6 (m, 4H), 3.42 (m, 4H), 2.52 (m, 2H), 2.25 (m, 2H), 2.1 (m, 2H) 2.0 (m, 4H), 1.6 (m, 2H)

Example 65

((1S,3R)-3-((3S,4S)-3-methoxy-tetrahydro-2H-pyran-4ylamino)cyclopentyl)((1S,4S)-5-(4-(trifluoromethyl)pyridin-2-yl)-2,5-diazabicyclo[2.2.1]heptan-2-yl)methanone ¹HNMR (400 MHz, CD3OD) δ 8.23 (d, J= 8Hz, 1H), 6.8 (d, J=8Hz, 1H), 6.72 (d, J=16 Hz, 1H), 5.0 (m, 1H), 4.2, (m, 1H), 3.9 (m, 1H), 3.85 (m, 1H), 3.8 (m, 4H), 3.4 (m, 4H), 3.30 (m, 3H), 3.2 (m, 4H), 2.0 (m, 4H), 1.8 (m, 4H)

124

Example 66

((1S,4S)-5-(3-fluoro-5-(trifluoromethyl)phenyl)-2,5-diaza-bicyclo[2.2.1]heptan-25 yl)((1S,3R)-3-((3S₎4S)-3-methoxy-tetrahydro-2H-pyran-4-ylamino)-1(tetrahydrofuran-3-yl)cyclopentyl)methanone

1HNMR (400Mhz, CD3OD), δ 6.6 (m, 3H), 5.0 (m, 1 H), 4.6 (m, 1 H), 4.25 (d.

J=4Hz, 1H), 4.0 (m, 1 H). 3.85 (m, 1H), 3.8 (m, 2H), 3.7 (m, 2H), 3.65 (m, 2H) 3.5 10 (m, 2H), 3.4 (3H), 3.2 (d, J=4Hz, 2H), 2.8 (m, 1 H), 2.6 (m, 1 H), 2.5 (m, 1 H), 2.35 (m, 1H), 2.2 (m, 1H), 2.1 (m, 2H), 2.05 (m, 2H), 2.0 (m, 2H), 1.8 (m, 2H),1.6 (m,

2H)

125

Préparation of Examples 67-71

Scheme. Prep of F-221

a) BoczO, DIPEA; b) NBS, CCI₄ then 10% Pd/C, H₂; c) CI-pyridine-CF₃, DIPEA, DMF; d) DAST, DOM, 0 °C; e) 4N HCI/dioxane, rt

Step 1. Préparation of (1 R,4S)-tert-butvl 7-hvdroxv-5-(4-(trifluoromethvl)pvridin2-vl)-2,5-diaza-bicyclo[2.2.nheptane-2-carboxvlate

To a solution of alcohol (2.48 g, 11.6 mmol) in DMSO (30 mL) was added 2chloro-5-(trifluoromethyl)pyridine (2.0 g, 11.0 mmol) and TEA (3.4 mL, 24.2 mmol). After heating the mixture at 95 °C ovemight the mixture was poured into H₂O and the aqueous mixture extracted 2X with ether. The organic extracts were dried (Na₂SO₄) and the solvent removed to give an oil, which after chromatography (silica, EtOAc:Heptane) gave the product (1.0 g). LC/MS (M+Na) = 382 exp, 382 obs.

Step 2. Préparation of (1 R,4S)-tert-butyl 7-fluoro-5-(4-(trifluoromethvl)pvridin-2yl)-2,5-diaza-bicyclo[2.2.1 lheptane-2-carboxylate

To a solution of alcohol in DCM at 0 °C was added DAST dropwise. After addition was complété the reaction mixture was allowed to warm to rt and stirred at rt overnight. The reaction mixture was poured into saturated NaHCOs/DCM and the layers separated. The organic layer was collected, dried (Na₂SO₄) and the solvent removed to give an oil, which after chromatography (silica,

126

EtOAc:Heptane) gave the desired product (750 mg). LC/MS (M+H) = 362.1491 exp, 362.1491 obs. 1H NMR (400 MHz, DMSO-de) δ ppm 8.31 (1 H, d, J=5.5 Hz), 6.85 - 6.96 (2 H, m), 5.35 (1 H, d, J=55.6 Hz), 4.90 (1 H, br. s.), 4.47 (1 H, d, J=22.7 Hz), 3.64(1 H, dd, J=10.2, 1.8 Hz), 3.47-3.57(1 H, m), 3.39-3.46(1 H, m), 3.26 - 3.32 (1 H, m), 1.39 (9 H, d, J=12.4 Hz).

Step 3. Préparation of (1 S.4R)-7-fluoro-2-(4-(trifluoromethvl)pvridin-2-vl)-2.5diaza-bicyclor2.2.11heptane

To a solution of (1R,4S)-tert-butyl 7-fluoro-5-(4-(trifluoromethyl)pyridin-2-yl)-2,5diaza-bicyclo[2.2.1]heptane-2-carboxylate (700 mg, 1.9 mmol) in dioxane (2 ml) was added 4N HCI/dioxane (5 mL). The reaction mixture was stirred at rt for 5 hr and then diluted with ether to give a precipitate that was filtered and collected to give the product as the HCl sait (506 mg). LC/MS (M+H) = 262.0967 exp, 262.1308 obs; 1H NMR (400 MHz, METHANOL-d₄) ô ppm 8.26 (1 H, d, J=6.1 Hz), 7.40 (1 H, s), 7.20 (1 H, dd, J=6.3, 1.0 Hz), 5.65 (1 H, dd, J=52.4,1.9 Hz), 5.28 (1 H, s), 4.70 (1 H, s), 3.92 - 4.09 (2 H, m), 3.66 - 3.81 (2 H, m), 3.62 (1 H, s)

Step 4. Préparation of ( 1 S,4R)-7-hydroxv-2-(4-(trifluoromethvl)pvridin-2-vl)-2.5diaza-bicyclo[2.2.1lheptane

To a solution of (1R,4S)-tert-butyl 7-hydroxy-5-(4-(trifluoromethyl)pyridin-2-yl)2,5-diaza-bicyclo[2.2.1]heptane-2-carboxylate (5 g, 14 mmol) in dioxane (5 ml) was added 4N HCI/dioxane (15 mL). The reaction mixture was stirred at rt for 6 hr and then diluted with ether to give a precipitate that was filtered and collected to give the product as the HCl sait (4.0 g). LC/MS (M+H) = 260.0 exp, 260.0 obs; 1H NMR (500 MHz, DMSO-d₆) δ ppm 3.42 - 3.50 (m, 1 H) 3.67 - 3.72 (m, 1 H) 3.75-3.82 (m, 1 H) 4.16 (br. s., 1 H) 4.50 (d, J=1.81 Hz, 1 H) 4.72 (br. s„ 1 H) 6.95 (d, J=5.13 Hz, 2 H) 8.31 (d, J=5.43 Hz, 1 H) 9.34 (br. s., 1 H) 9.75 (br. s., 1 H)

127

Scheme 5

X= F, OH

a) DCM, TEA, R1R2NH, rt; b) 50% NaOH, EtOH, rt; c)

Scheme 6

128

X= F, OH

a) DCM, TEA, R1R2NH. rt; b) 50% NaOH, EtOH, rt; c) DAST, DCM, 0 oC; d) 4N HCI/dioxane; e) Pyr-CI Et3N, DMSO, 100 oC

Example 67

((1R,4R,7S)-7-fluoro-5-(4-(trifluoromethyl)pyridin-2-yl)-2,5-diazabicyclo[2.2.1]heptan-2-yl)((1S,3R)-1-isopropyl-3-((3S,4S)-3-methoxy-tetrahydro2H-pyran-4-ylamino)cyclopentyl)methanone

Prepared in a manner analogous to example 1, except using (1 R,4R,7R)-7fluoro-2-(4-(trifluoromethyl)pyridin-2-yl)-2,5-diaza-bicyclo[2.2.1]heptane as the coupling amine. LC/MS (M+H) = 529 exp, 529 obs; 1H NMR (400 MHz, DMSOd₆) δ ppm 8.31 (1 H, d, J=5.1 Hz), 6.92 (2 H, d, J=5.1 Hz), 5.39 (1 H, d, J=55.6

129

Hz), 4.81 - 4.98 (2 H, m), 3.78 - 3.88 (1 H, m), 3.66 - 3.75 (2 H, m), 3.57 - 3.65 (1 H, m), 3.37 - 3.43 (1 H, m), 3.23 - 3.31 (2 H, m), 3.15 - 3.22 (4 H, m), 3.06 - 3.10 (1 H, m), 2.95 - 3.03 (1 H, m), 2.68 - 2.77 (1 H, m), 2.22 - 2.32 (1 H, m), 1.93 2.09 (2 H, m), 1.61 -1.72 (2 H, m), 1.35 - 1.53 (3 H, m), 1.21 -1.29 (1 H, m), 1.08 -1.20 (1 H, m), 0.82 (2 H, d, 7=6.2 Hz), 0.64 - 0.77 (4 H, m)

Example 68

((1R,4R,7S)-7-hydroxy-5-(4-(trifluoromethyl)pyridin-2-yl)-2,5-diazabicyclo[2.2.1]heptan-2-yl)((1S,3R)-1-isopropyl-3-((3S,4S)-3-methoxy-tetrahydro2H-pyran-4-ylamino)cyclopentyl)methanone

To a solution of the (1S,3R)-1-isopropyl-3-(2,2,2-trifluoro-N-((3S,4S)-3-methoxytetrahydro-2H-pyran-4-yl)acetamido)cyclopentanecarbonyl chloride (185mg, 0.463mmol) in CH₂CI₂ (2mL) was added Et₃N (0.161ml, 1.16mmol) and (1R,4R,7R)-7-hydroxy-2-(4-(trifluoromethyl)pyridin-2-yl)-2,5-diazabicyclo[2.2.1]heptane (100mg, 0.386mmol). The mixture was stirred for 4 hrs. at RT. The solution was then washed with NaHCO₃ (1 X 3ml), and brine (1 X3ml). The organic layer was then dried over MgSO4, filtered and concentrated, purified by chromatopraphy with (100% EtOAc- 70% MeOH/EtOAc) to yield TFA protected product, which was then treated with 2N NaOH (0.5ml, 1mmol) in THF (2ml) at RT for 4 hrs., then purified by chromatography with (100% EtOAc-100% MeOH) to yield 60 mg desired product ( 30% ). LC/MS (M+H) = 527.2845 exp, 527.2996 obs; 1H NMR (400 MHz, DMSO-d₆) δ ppm 0.64 - 0.76 (m, 4 H) 0.77 0.88 (m, 3 H) 1.13 (d, 7=1.83 Hz, 1 H)1.34(br. s., 1 H) 1.36 - 1.48 (m, 3 H) 1.66 (dt, 7=7.69, 3.84 Hz, 1 H) 1.77 - 1.90 (m, 1 H) 1.99 (dt, 7=13.55, 6.77 Hz, 2 H) 2.29 (dd, 7=12.08, 8.05 Hz, 1 H) 2.73 - 2.79 (m, 1 H) 2.97 - 3.04 (m, 1 H) 3.07 17116

130

3.14 (m, 2 H) 3.15 - 3.24 (m, 5 H) 3.59 (br. s., 1 H) 3.61 - 3.72 (m, 2 H) 3.75 3.88 (m, 1 H) 4.26 (d, J=0.73 Hz, 1 H) 4.44 - 4.55 (m, 2 H) 5.72 (d, 7=3.29 Hz, 1

H) 6.75 - 6.85 (m, 2 H) 8.27 (d, 7=5.49 Hz, 1 H)

Example 69

((1R,4R)-7-fluoro-5-(4-(trifluoromethyl)pyridin-2-yl)-2,5-diazabicyclo[2.2.1]heptan-2-yl)((1S,3R)-1-isopropyl-3“((3S,4S)-3“methoxy-tetrahydro2H-pyran-4-ylamino)cyclopentyl)methanone

2,2,2-trifluoro-N-((1R,3S)-3-((1R,4R,7S)-7-hydroxyl-2-(4-(trifluoromethyl)pyridin2-yl)-2,5-diaza-bicyclo[2.2.1]heptane-5-carbonyl)-3-isopropylcyclopentyl)-N((3S,4S)-3-methoxy-tetrahydro-2H-pyran-4-yl)acetamide (30 mg, 0.057 mmol) in CH2CI2 (2ml) was cooled to 0 °C and DAST (0.025ml, 0.086mmol) was added to the solution. The reaction mixture was stirred overnight and purified by chromatography (10% EtOAc to 100% MeOH) to yield desired product (16mg, 32%). LC/MS (M+H) = 529.2802 exp, 529.3112 obs; 1H NMR (400 MHz, DMSOd_e) Ô ppm 0.67 - 0.78 (m, 5 H) 0.82 (d, 7=6.59 Hz, 3 H) 1.23 (br. s., 1 H) 1.50 (d, 7=3.66 Hz, 3 H) 1.75 (d, 7=4.39 Hz, 2 H) 1.94 - 2.03 (m, 1 H) 2.24 - 2.32 (m, 1 H) 2.98 (s, 1 H) 3.12 (br. s., 1 H) 3.18 - 3.23 (m, 4 H) 3.31 - 3.34 (m, 1 H) 3.40 (d, 7=10.98 Hz, 1 H) 3.62 (d, 7=12.08 Hz, 1 H) 3.72 (d, 7=10.62 Hz, 3 H) 3.80 - 3.92 (m, 1 H) 4.87 (br. s„ 1 H) 4.90 - 4.96 (m, 1 H) 5.31 (d, 7=1.83 Hz, 1 H) 5.40 5.46 (m, 1 H) 6.86 - 6.94 (m, 2 H) 8.31 (d, 7=5.49 Hz, 1 H)

I3l

Example 70

(1R,4R,7S)-tert-butyl 7-hydroxy-5-((1S)-1-isopropyl-3-(3-methoxy-tetrahydro-2Hpyran-4-ylamino)cyclopentanecarbonyl)-2,5-diaza-bicyclo[2.2.1]heptane-2carboxylate

To a solution of the (1S,3R)-1-isopropyl-3-(2,2,2-trifluoro-N-((3S,4S)-3-methoxytetrahydro-2H-pyran-4-yl)acetamido)cyclopentanecarboxylic acid (0.95g, 2.5mmol) in CH2CI2 (5mL) under N2 at 0 °C is added oxalyl chloride (623mg, 4.98mmol) and DMF (3 drops). The mixture is allowed to warm to RT and stir for 2 hr, It was then concentrated. The acid chloride was redissolved in DCM (10ml), cooled to 0 °C, and treated with (1R,4R,7S)-tert-butyl 7-hydroxy-2,5-diazabicyclo[2.2.1]heptane-2-carboxylate (534mg, 0.249mmol), followed by Et3N (0.1ml, 0.75mmol). The resulting mixture was warmed to RT and stirred for 2hrs. The solution was then washed with NaHCOa (1 X5ml), and brine (1 X 5ml). The organic layer was then dried over MgSO4, filtered and concentrated, purified by chromatography (100% EtOAc- 60% MeOH/EtOAc) to yield crude intermediate which was dried and concentrated. Then it was dissolved in EtOH (2ml) and treated with NaOH ( 3ml, 7.5mmol) overnight. The reaction mixture was then purified by chromatography (100% EtOAc-100% MeOH) to yield desired product (300mg, 25%). LC/MS (M+H) = 482.3230 exp, 482.3334 obs; 1H NMR (400 MHz, DMSO-d₆) ô ppm 0,67 (dd, J=6.95, 3.66 Hz, 3 H) 0.80 (dd, J=6.59, 2.93 Hz, 3 H) 1.22 (br. s., 1 H) 1.35 -1.41 (m, 12 H) 1.43 - 1.48 (m, 2 H) 1.55 -1.68 (m, 2

H) 1.85-1.93 (m, 1 H) 1.95-2.04 (m, 1 H) 2.16 - 2.28 (m, 1 H) 2.66-2.80 (m,1

H) 2.97 - 3.02 (m, 1 H) 3.03 - 3.10 (m, 2 H) 3.17 - 3.29 (m, 5 H) 3.37 - 3.47 (m,1

H) 3.47 - 3.58 (m, 1 H) 3.61 - 3.74 (m, 1 H) 3.81 - 3.89 (m, 1 H) 3.94 - 4.04 (m,2

H) 4.24 (br. s., 1 H) 5.65 - 5.77 (m, 1 H)

132

Example 71

tert-butyl 7-fluoro-5-((1S,3R)-1-isopropyl-3-((3S,4S)-3-methoxy-tetrahydro-2H5 pyran-4-ylamino)cyclopentanecarbonyl)-2,5-diaza-bicyclo[2.2.1]heptane-2carboxylate tert-butyl 7-hydroxy-5-((1S)-1-isopropyl-3-(2,2,2-trifluoro-N-(3-methoxytetrahydro-2H-pyran-4-yl)acetamido)cyclopentanecarbonyl)-2,5-diaza10 bicyclo[2.2.1]heptane-2-carboxylate (100 mg, 0.173 mmol) in CH2CI2 (2 ml) was cooled to 0 °C and DAST (0.036ml, 0.259mmol) was added to the solution. The reaction mixture was stirred overnight and purified by chromatography (10% EtOAc to 100% MeOH) to yield TFA protected product, which was then treated with 2.5N NaOH (0.014ml, 0.5mmol) in THF (2ml) at RT for 4 hrs., then purified by chromatopraphy with (100% EtOAc-100% MeOH) to yield 12 mg desired product ( 14% ). LC/MS (M+H) = 464.3187 exp, 484.3197 obs; 1H NMR (400 MHz, DMSO-d₆) δ ppm 0.63 - 0.72 (m, 3 H) 0.81 (dd, J=7.14, 1.65 Hz, 3 H) 1.22 (br. s., 1 H) 1.36 - 1.48 (m. 13 H) 1.56 -1.68 (m, 2 H) 1.85 - 1.96 (m, 2 H) 2.16 2.28 (m, 1 H) 2.68 - 2.79 (m, 1 H) 2.95 - 3.05 (m, 1 H) 3.15 (br. s., 1 H) 3.17 20 3.29 (m, 7 H) 3.52 (d, J=10.62 Hz, 2 H) 3.66 - 3.74 (m, 1 H) 3.82 - 3.90 (m, 1 H)

4.27 - 4.37 (m, 1 H) 4.69 (br. s., 1 H) 5.08 - 5.35 (m, 1 H)

Bioloqical Data

CCR2 in vitro assays

The capacity of the novel compounds of the invention to antagonize chemokine receptor (e.g., CCR2) function can be determined using a suitable

133 screen (e.g., high through-put assay). For example, an agent can be tested in an extracellular acidification assay, calcium flux assay, ligand binding assay or chemotaxis assay (see, for example, Hesselgesser et al., J Biol. Chem. 273(25):15687-15692 (1998); WO 00/05265 and WO 98/02151).

In a suitable assay, a CCR2 protein which can be isolated or recombinantly derived is used which has at least one property, activity or functional characteristic of a mammalian CCR2 protein. The spécifie property can be a binding property (to, for example, a ligand or inhibitor), a signalling activity (e.g., activation of a mammalian G protein, induction of rapid and transient increase in the concentration of cytosolic free calcium [Ca++]i, cellular response function (e.g., stimulation of chemotaxis or inflammatory mediator release by leukocytes), and the like.

In an example binding assay, a composition containing a CCR2 protein or variant thereof is maintained under conditions suitable for binding. The CCR2 receptor is contacted with a compound to be tested, and binding is detected or measured.

In an example cell-based assay, cells are used which are stably or transiently transfected with a vector or expression cassette having a nucleic acid sequence which encodes the CCR2 receptor. The cells are maintained under conditions appropriate for expression of the receptor and are contacted with an agent under conditions appropriate for binding to occur. Binding can be detected using standard techniques. For example, the extent of binding can be determined relative to a suitable control. Also, a cellular fraction, such as a membrane fraction, containing the receptor can be used in lieu of whole cells.

Détection of binding or complex formation in an assay can be detected directly or indirectly. For example, the agent can be labeled with a suitable label (e.g., fluorescent label, label, isotope label, enzyme label, and the like) and binding can be determined by détection of the label. Spécifie and/or compétitive binding can be assessed by compétition or displacement studies, using unlabeled agent or a ligand as a competitor.

134

The CCR2 antagonist activity of compounds of the invention can be reported as the inhibitor concentration required for 50% inhibition (IC50 values) of spécifie binding in receptor bindîng assays using 1251-labeled MCP-1, as ligand, and Peripheral Blood Mononuclear Cells (PBMCs) prepared from normal human whole blood via density gradient centrifugation. Spécifie binding is defined as the total binding (e.g., total cpm on filters) minus the non-specific binding. Nonspecific binding is defined as the amount of cpm still detected in the presence of excess unlabeled competitor (e.g., MCP-1).

CCR2 binding IC_S0

Human PBMCs were used to test compounds of the invention in a binding assay. For example, 200,000 to 500,000 cells were incubated with 0.1 to 0.2 nM 1251-labeled MCP-1, with or without unlabeled competitor (10 nM MCP-1) or various concentrations of compounds to be tested. 1251-labeled MCP-1, were prepared by suitable methods or purchased from commercial vendors (Perkin Elmer, Boston MA). The binding reactions were performed in 50 to 250 pL of a binding buffer consisting of 1M HEPES pH 7.2, and 0.1% BSA (bovine sérum albumin), for 30 minutes at room température. The binding reactions were terminated by harvesting the membranes by rapid filtration through glass fiber filters (Perkin Elmer) which was presoaked in 0.3% polyethyleneimine or Phosphate Buffered Saline (PBS). The filters were rinsed with approximately 600 pL of binding buffer containing 0.5 M NaCI or PBS, then dried, and the amount of bound radioactivity was determined by counting on a Gamma Counter (Perkin Elmer).

More specifically, the following assay may be employed to détermine IC50 values for the compounds of the présent invention.

hCCR2 (125-1 hMCP-1) Small Molécule Binding Assay

The following reagents and supplies hâve been used in the preceding assay:

135

MCP-1

Biosource # PHC1013 mg reconstitute with 2 ml binding buffer (0.5 mg per ml or 60 uM)

1251 MCP-1

Perkin Elmer#NEX332 uCi reconstitute with 0.2 ml PBS

RPMI 1640 with L-glutamine

MediaTech/Cellgro # 10-040-CM

BSA

Sigma #A2153

HEPES

M solution

MediaTech/Cellgro # 25-060-CL

NaCI

Sigma # S7653

SUPPLIES

Muliscreen BV Filter Plates

Millipore # MABVN1250

Multiscreen Punch Tips

Millipore #MADP19650

Multiscreen Filtration System Vacuum Manifold

136

Millipore # MAVM0960R

Normal human leukophoresis pack contents, available from Biological Specialty Corporation, Colmar, PA., is diluted 1:1 with PBS, divided into 50ml conical tubes (preferably less than 40mls per tube), and underlayed with 10 mis of Ficoll-Paque PLUS (GE Healthcare 17-1440-02). The tubes are centrifuged at 2800rpm in a clinical centrifuge at room température for 30 minutes, with no brake. The plasma layer is suctioned, and the buffycoat layer is collected. The collected buffycoat layer is washed twice with 50 mis PBS, and centrifuged at 1400rpm with brake. The cells are counted. The cells are then diluted to 1x10⁷ cells per ml in binding buffer.

well plates (such as, for example, Millipore MultiScreen96 plates) are pre-wet with about 100 pl binding buffer (RPMI+0.1% BSA + 20mM HEPES), and blotted just prior to compound addition.

Plates with compound to be tested may be advantagously stored or managed with an automated System, such as, for example,.TelCel (Hamilton Storage Technologies, Inc., 103 South Street, Hopkinton, MA 01748 USA) pl of 50 pM test compounds in 100% DMSO are dotted onto U-bottom polypropylene 96-well plates.

245 pl per well of Binding Buffer is added to each well for 1 pM compound concentration in 2% DMSO.

50pl of 1pM compound is transferred to pre-wetted Millipore plates. 50 pl per well of 1x10E7 cells per ml of freshly prepared human PBMCs are added.

The samples are pre-incubated at room température for 30 minutes or 1 hour.

pl of 450pM 125-l-hMCP-1 (Perkin-Elmer/NEN cat#NEX332025UC) is added for a final concentration 125-l-hMCP-1 of 150pM per well.

The final test compound concentration is 0.333uM in 150pl total volume with 0.67% DMSO in ail wells. Controls for this assay consist of 0% inhibition and

137

100% inhibition with 1 uM hMCP-1 (saturating conditions). Ail wells are run in duplicate. Controls may be in replicates of eight.

The samples are incubated at room température for 30 minutes.

The buffer is suctioned through the Millipore plates. The plates are washed three times with Wash Buffer (PRMI + 0.1% BSA + 20mM HEPES + 0.4 M NaC).

The plate underdrain is removed. The plate filters are allowed to dry. The filters are then punched out into plastic tubes.

Finally, the sample is counted on a Gamma Counter.

Ail IC50 plates were repeated for an n=2.

The following table summarizes the IC₅₀ values identified through the described assay.

138

Table 1. Biological Data

Example	IC50 (nM) 30 min preinc	IC50 (nM) 1 hr preinc
1	2.47
2	3.16
3	17.8
4	3.98
5	13.3
6	1.73
7	17.1
8	10.7
9	30.7
10	83.8
11	6.72	2.44
12	11.1	1.7
13	25.4	3.68
14	10.4	2.3
15	40	4.6
16	9.95	1.1
17	13.7	5.8
18	37.6	8.5
19	20.4	6.35
20	8.59
21		3.1
22		1.1
23		0.94
24	79.7	8.87
25	6.58	0.93
26	29.3	7.85
27	16.2	2.04
28		1.88
29		2.75
30	32.9	11.8
31		6.35
32	13.8	11.7
33	57.3	11.7
34	66.5	6.94
35	28.4	5.52
36	12.6	2.19
37		3.68
38	68.7	2.02
39	82.9	5.81
40	18.3

I39

Example	IC50 (nM) 30 min preinc	IC50 (nM) 1 hr preinc
41	63.6
42	70.9
43	47.3
44		8.1
45	25.3
46	3.19
47	6.16
48	25.3
49	35.8
50	7.14
51	22.8
52	5.4
53	27.6
54	8.46
55	28.3
56	32.8
57	4.34
58	38.5
59	17.7
60	2.4
61	5.9
62	1.3
63		1.1
64		4.7
65		1.5%@300nM
66	1.7
67	3.4
68	5.4
69	4.3
70	8.7
71	3.7

Diabetic Nephropathy Rat Mode!

A rat model of diabetic nephropathy was used to détermine the rénal protective effect of pharmacological inhibition of CCR2. The streptozotocin (STZ)-induced diabetic rat model has been extensively used for studying the progression of diabetic rénal disease. STZ injection causes immédiate destruction of pancreatic β-cells, consequently hyperglycémie and progression of nephropathy similar to that seen in human diabètes mellitus. Diabètes was induced in male Wistar rats by single administration of STZ (45 mg/kg, iv). Three

140 days after the induction of diabètes, fasting blood glucose levels were assessed. Animais with fasting blood glucose levels higher than 200mg/dL were included in the study. Ail the treatments were then initiated. 1,5-anhydro-2,3-dideoxy-3{[(1 R,3S)-3-isopropyl-3-({(1S,4S)-5-[6-(trifluoromethyl)pyrimidin-4-yl]-2,5diazabicyclo[2.2.1]hept-2-yl}carbonyl)cyclopentyl]amino}-4-O-methyl-D-erythropentitol was administered at doses of 0.03, 0.3, 1, and 10 mg/kg (mpk) (in the chow) for 11 weeks. 24-hour urine collections were obtained at 1,4, 8, and 11 weeks after the treatment for assessing 24-hour urinary albumin excrétion (UAE). As expected, following the induction of diabètes there was a persistent élévation in UAE over the 11 week course of this study. 1,5-anhydro-2,3-dideoxy-3{[(1R,3S)-3-isopropyl-3-({(1S,4S)-5-[6-(trifluoromethyl)pyrimidin-4-yl]-2,5diazabicyclo[2.2.1]hept-2-yl}carbonyl)cyclopentyl]amino}-4-O-methyl-D-erythropentitol treatment significantly reduced UAE at doses of 0.3 mpk (1.73±0.69 mg/24h), 1 mpk (1.09±0.20 mg/24h), and 10 mpk (0.71±0.22 mg/24h) compared to untreated STZ rats (4.3211.34 mg/24h) at week 8, and at ail doses (2.1711.31, 1.9610.85, 1.6610.65, and 1.0210.32 mg/24h, at0.03, 0.3, 1, and 10 mpk dose, respectively, vs. 6.3612.08 mg/24h, untreated STZ rats) at week 11. These data demonstrate that pharmacological inhibition of CCR2 provides rénal protection during the development and progression of diabetic nephropathy and support CCR2 antagonism as a new therapeutic strategy to treat diabetic rénal disease.

Claims (13)

1. Claims

   What is claimed is:

   1. A compound of Formula l(a) or l(b):

   or a pharmaceutically acceptable sait thereof, wherein:

   A is O or CF₂;

   W is CR¹³R¹⁴, C(O), CHOR¹⁵, CHF, CF₂, O or S

   R¹ is H or (Ci-C₆)alkyl optionally substituted by 1-3 substituents selected from halo, OH, CO₂H, CO₂-(Ci-C₆) alkyl, or (C1-C3) alkoxy;

   R² and R³ are each, independently, H, (Ci-C6)alkyl, (Ci-C6) haloalkyl, halo, (C3-C6) cycloalkyl, CN, OH, CO2R, OCOR¹²; wherein said (CvCeJalkyl is optionally substituted with one or more substituents selected from F, (C1-C3) alkoxy, OH.CN or CO2R¹²;

   R⁸ and R⁹ are each, independently, H, (Ci-C6)alkyl, (Ci-Cejhaloalkyl, (C3C6)cycloalkyl, CN, OH, CO2R, OCOR¹²; wherein said (Ci-Ce)alkyl is optionally substituted with one or more substituents selected from F, (Ci-C3) alkoxy, OH, CN or CO₂R¹²;

   142

   R² and R⁹, taken together may form a 5-8 membered ring;

   R⁴ and R⁷, taken together may form a 5-8 membered ring;

   R⁴ and R⁵ are each, independently, H, CN, (Ci-C6)alkyl, halo, (Cr C3)alkoxy, (Ci-C3)haloalkoxy, (C3-C6)cycloalkyl, OH, CO2R¹², OCOR¹² , wherein said (Ci-C6)alkyl is optionally substituted with one or more substituents selected from F, (Ci-C₃) alkoxy, OH or CO₂R¹²;

   R⁶ and R⁷ are each, independently, H, CN, (Ci-C6)alkyl, halo, (C1C3)alkoxy, (Ci-C3)haloalkoxy, (C3-C6)cycloalkyl, OH, CO2R¹², OCOR¹² , wherein said (Ci-C6)alkyl is optionally substituted with one or more substituents selected from F, (Ci-C₃) alkoxy, OH or CO₂R¹²;

   or R² and R³ together with the carbon atom to which they are attached form a 3-7 membered spirocyclyl group;

   or R⁴ and R⁵ together with the carbon atom to which they are attached form a 3-7 membered spirocyclyl group;

   or R³ and R⁴ together with the C atoms to which they are attached form a fused 3-7 membered cycloalkyl group or 3-7 membered heterocycloalkyl group;

   R¹⁰ is (Ci-C6)alkyl, (Ci-Ce)haloalkyl, (C-i-Ce)hydroxyalkyl, (Ci-C₆)alkoxy, (C₃-C₆)cycloalkyl, hydroxy(C₃-C₆)cycloalkyl, alkoxy cycloalkyl, OH, (C1Cs)heterocyclyl, amino, aryl or CN,

   R¹¹ is aryl or heteroaryl, said R¹¹ optionally, independently substituted by one or more (CrC₆) alkyl, halo, (CiC₆)haloalky (Ci-C₃)alkoxy, OH, amino, C(O)NH₂, NH₂SO₂, SF₅,or CN;

   R¹²is H, (Ci-C₄)alkyl, or (C₃-C6)cycloalkyl;

   R¹³ is H, halo, (Ci-C4)alkyl, (C_rC₄)alkoxy,or OH;

   R¹⁴ is H, halo, (C₁-C₄)alkyl, (C_rC₄)alkoxy or OH; and

   R¹⁵ is H ,(Ci-C₆)alkyl or (C₃-C₆)cycloalkyl.
2. 2. A compound of Formula II:

   N /

   R’» I W or a pharmaceutically acceptable sait thereof, wherein:

   R¹ is H, (Ci-C₆)alkyl or (C₃-C_B)cycloalkyl, said (C-f-C₆)alkyl optionally substituted by halo, CN, C(O)OH or OH;

   R² is (Ci-C₆)alkyl (C-|-C₆)haioalkyl or (C₃-C₆)cycloalkyl;

   R³ R⁴, R⁵, R⁶, R⁷, R⁸ and R⁹ are independently H, C_rC₄ alkyl, CN, halo or amino;

   144

   R¹⁰ is (CrCeJalkyl, (Ci-C₆)haloalkyl, (Ci-C₆)hydroxyalkyl, (Ci-C₆)alkoxy, (C₃C₆)cycloalkyl, hydroxy(C₃-C6)cycloalkyl, alkoxy (C₃-C6)cycloalkyl, OH, (Cr C₅)heterocyclyl, amino, aryl or CN;

   R¹¹ is aryl or heteroaryl, said R¹¹ optionally, independently substituted by one or more (CrCe) alkyl, halo, (CrCe)haloalky (C₁-C₆)alkoxy, OH, amino, C(O)NH₂, NH₂SO₂, SF₅ orCN;

   W is CR¹³R¹⁴, C(O), CHOR¹⁵, CHF, CF₂, O or S;

   R¹³ and R¹⁴ are independently H halo, (Ci-C₄)alkyl, (Ci-C₄)alkoxy,or OH; and

   R¹⁵ is H, (Ci-C₆)alkyl or (C₃-C₆)cycloalkyl.
3. 3. The compound of claim 2 or a pharmaceutically acceptable sait thereof, wherein:

   R¹ is H or (Ci-C₆)alkyl

   R² is (Ci-C₆)alkyl or (Ci-C6)haloalkyl

   R³, R⁴, R⁵, R⁶, R⁷, R⁰ and R⁹ are each H;

   , wherein X, Y, Z, Q and D are independently N or CR¹⁶, and wherein 0, 1, 2 or 3 of X, Y, Z, Q and D are N; and

   145 wherein T, U and V are independently selected from CH, N, S, or O, provided that T and U are not both simultaneously O or S;

   each R¹⁶ is independently H, halo, (Ci-Ce)alkyl, (CrCeJhaloalkyl, (CrC₆)alkoxy or CN;

   R¹⁷ is H, (Ci-C₆)alky, (Ci-Cejhaloalky, or (C_rCe)alkoxy; and

   W is CR¹³R¹⁴, C(O), CHOR¹⁵, CHF or CF₂.
4. 4. The compound of claim 3, or a pharmaceutically acceptable sait thereof, wherein R² is methyl.
5. 5. The compound of claim 4 wherein R¹⁰ is

   H or (CrC₆) alkyl.
6. 6. The compound of claim 3, or a pharmaceutically acceptable sait thereof, wherein R is

   147

   5 7. The compound of claim 5 or a pharmaceutically acceptable sait thereof, wherein R¹¹ is
7. 9. A compound selected from the group consisting of:

   1,5-anhydro-2,3-dideoxy-3-{[( 1 R,3S)-3-ethyl-3-({( 1 S,4S )-5-[4(trifluoromethyl)pyridin-2-yl]-2,5-diazabicyclo[2.2.1]hept-2yl}carbonyl)cyclopentyl]amino}-4-O-methyl-D-erythro-pentitol;

   1,5-anhydro-2,3-dideoxy-3-{[(1R,3S)-3-ethyl-3-({(1S,4S)-5-[4(trifluoromethyl)pyrimidin-2-yl]-2,5-diazabicyclo[2.2.1]hept-2yl}carbonyl)cyclopentyl]amino}-4-O-methyl-D-erythro-pentitol;

   1,5-anhydro-2,3-dideoxy-3-{[( 1 R,3S )-3-ethyl-3-({( 1S ,4S )-5-[6(trifluoromethyl)pyrimidin-4-yl]-2₁5-diazabicyclo[2.2.1]hept-2yl}carbonyl)cyclopentyl]amino}-4-O-methyl-D-erythro-pentitol;

   1,5-anhydro-2,3-dideoxy-3-{[(1 R,3S)-3-ethyl-3-({(1S,4S)-5-[6(tnfluoromethyl)pyrazin-2-yl]-2,5-diazabicyclo[2.2.1 ]hept-2yl}carbonyl)cyclopentyl]amino}-4-O-methyl-D-erythro-pentitol;

   1,5-anhydro-2,3-dideoxy-3-{[( 1R ,3S )-3-ethyl-3-( {( 1S ,4S)-5-[2(trifluoromethyl)pyrimidin-4-yl]-2,5“diazabicyclo[2.2.1]hept-2yl}carbonyl)cyclopentyl]amino}-4-O-methyl-D-erythro-pentitol;

   1,5-anhydro-2,3-dideoxy-3-{[(1R,3S)-3-ethyl-3-({(1S,4S)-5-[6(trifluoromethyl)pyridin-2-yl]-2,5-diazabicyclo[2.2.1]hept-2yl}carbonyl)cyclopentyl]amino}-4-O-methyl-D-erythro-pentltol;

   150

   1 _t5-anhydro-2,3-dideoxy-3-{[(1R,3S)-3-ethyl-3-({(1S,4S)-5-[2(trifluoromethyl)pyridin-4-yl]-2,5-diazabicyclo[2.2.1]hept-2yljcarbonyl )cyclopentyl]amino}-4-O-methyl-D-erythro-pentitol ;

   1,5-anhydro-2,3-dideoxy-3-{[(1R_I3S)-3-ethyl·3-({(1S,4S)-5-[3-fluoro-4(trifluoromethyl)pyridin-2-yl]-2,5-diazabicyclo[2.2.1]hept-2yl}carbonyl)cyclopentyl]amino}-4-O-methyl-D-erythro-pentitol;

   1,5-anhydro-2,3-dideoxy-3-{[(1 R,3S)-3-methyl-3-({(1 S,4S)-5-[4(trifluoromethyl)pyridin-2-yl]-2,5-diazabicyclo[2.2.1]hept-2yl}carbonyl)cyclopentyl]amino}-4-O-methyl-D-erythro-pentitol;

   1,5-anhydro-2,3-dideoxy-3-{[(1R,3S)-3-methyl-3-({(1S,4S)-5-[6(trifluoromethyl)pyrazin-2-yl]-2,5-diazabicyclo[2.2.1]hept-2yl}carbonyl)cyclopentyl]amino}-4-O-methyl-D-erythro-pentitol;

   1,5-anhydro-2,3-dideoxy-3-{[(1R,3S)-3-isopropyl-3-({(1S,4S)-5-[4(trifluoromethyl)pyridÎn-2-yl]-2,5-diazabicyclo[2.2.1]hept-2yl}carbonyl)cyclopentyl]amino}-4-O-methyl-D-erythro-pentitol;

   1,5-anhydro-2,3-dideoxy-3-{[(1R,3S)-3-isopropyl-3-({(1S,4S)-5-[4(trifluoromethyl)pyrimidin-2-yl]-2,5-diazabicyclo[2.2.1]hept-2yl}carbonyl)cyclopentyl]amino}-4-O-methyl-D-erythro-pentitol;

   1,5-anhydro-2,3-dideoxy-3-{[(1 R,3S)-3-isopropyl-3-({(1S,4S)-5-[6(trifluoromethyl)pyrimidin-4-yl]-2,5-diazabicyclo[2.2.1]hept-2yl}carbonyl)cyclopentyl]amino}-4-O-methyl-D-erythro-pentitol;

   1,5-anhydro-2,3-dideoxy-3-{[( 1 R,3S)-3-isopropyl-3-({( 1 S,4S)-5-[6(trifluoromethyl)pyrazin-2-yl]-2,5-diazabicyclo[2.2.1]hept-2yl}carbonyl)cyclopentyl]amino}-4-O-methyl-D-eîythro-pentitol;

   I5l

   1,5-anhydro-2,3-dideoxy-3-{[(1 R,3S)-3-isopropyl-3-({(1S,4S)-5-[2“ (trifluoromethyl)pyrimidin-4-yl]-2,5-diazabicyclo[2.2.1]hept-2yl}carbonyl)cyclopentyl]amino}-4-O-methyl-D-erythro-pentitol;

   1,5-anhydro-2,3-dideoxy-3-{[(1 R,3S)-3-isopropyl-3-({(1S,4S)-5-[6(trifluoromethyl)pyridin-2-yl]-2,5-diazabicyclo[2.2. 1 ]hept-2yl}carbonyl)cyclopentyl]amino}-4-O-methyl-D-erythro-pentitol;

   1,5-anhydro-2,3-dideoxy-3-{[(1 R,3S)-3-isopropyl-3-({(1S,4S)-5-[2(trifluoromethyl)pyridin-4-yl]-2,5-diazabicyclo[2.2.1]hept-2yl}carbonyl)cyclopentyl]amino}-4-O-methyl-D-erythro-pentitol;

   1,5-anhydro-2,3-dideoxy-3-{[(1R,3S)-3-isopropyl-3-({(1S,4S)-5-[5(trifluoromethyl)pyridazin-3-yl]-2,5-diazabicyclo[2.2.1]hept-2yl}carbonyl)cyclopentyl]amino}-4-O-methyl-D-erythro-pentitol;

   I.S-anhydro-S-iniR.SSJ-S-isopropyl-S-titlS^SJ-S-P.e-bisitnfluoromethyOpyridin4-yl]-2₁5-diazabicyclo[2.2.1]hept-2-yl}carbonyl)-3-isopropylcyclopentyl]amino}2,3-dideoxy-4-O-methyl-D-erythro-pentitol;

   1,5-anhydro-2,3-dideoxy-3-{[(1R,3S)-3-({(1S,4S)-5-[3-fluoro-4(trifluoromethyl)pyridin-2-yl]-2,5-diazabicyclo[2.2.1]hept-2-yl}carbonyl)-3isopropylcyclopentyl]amino}-4-O-methyl-D-erythro-pentitol;

   1,5-anhydro-2,3-dideoxy-3-{[(1R,3S)-3-isopropyl-3-({(1S,4S)-5-[6-methyl-4(trifluoromethyl)pyridin-2-yl]-2,5-diazabicyclo[2.2.1 ]hept-2yl}carbonyl)cyclopentyl]amino}-4-O-methyl-D-erythro-pentitol;

   152

   1,5-anhydro-2,3-dideoxy-3-{[( 1 R,3S)-3-isopropyl-3-({( 1 S,4S )-5-[3(trifluoromethyl)phenyl]-2,5-diazabicyclo[2.2.1]hept-2yl}carbonyl)cyclopentyl]amino}-4-O-methyl-D-erythro-pentitol;

   1,5-anhydro-2_l3-dideoxy-3-{[(1R,3S)-3-({(1S,4S)-5-[3-fluoro-5(trifluoromethyl)phenyl]-2,5-diazabicyclo[2.2.1]hept-2-yl}carbonyl)-3isopropylcyclopentyl]amino}-4-O-methyl-D-erythro-pentitol;

   1,5-anhydro-2,3-dideoxy-3-{[(1R,3S)-3-(2₁2-difluoroethyl)-3-({(1S,4S)-5-[6(trifluoromethyl)pyrimidin-4-yl]-2,5-diazabicyclo[2.2.1]hept-2yl}carbonyl)cyclopentyl]amino}-4-O-methyl-D-erythro-pentitol;

   1,5-anhydro-2,3-dideoxy-3-{[(1 R,3S )-3-(2,2-difluoroethyl)-3-({(1 S,4S)-5-[4(trifluoromethyl)pyridin-2-yl]-2,5-diazabicyclo[2.2.1]hept-2yl}carbonyl)cyclopentyl]amino}-4-O-methyl-D-erythro-pentitol;

   1_l5-anhydro-2,3-dideoxy-3-{[(1R,3S)-3-(2,2-difluoroethyl)-3-({(1S,4S)-5-[2(trifluoromethyl)pyridin-4-yl]-2,5-diazabicyclo[2.2.1]hept-2yl}carbonyl)cyclopentyl]amino}-4-O-methyl-D-erythro-pentitol;

   1,5-anhydro-2,3-dideoxy-3-{[(1 R,3S)-3-(2,2-difluoroethyl)-3-({(1S,4S)-5-[4(trifluoromethyl)pyrimidin-2-yl]-2,5-diazabicyclo[2.2.1]hept-2yl}carbonyl)cyclopentyl]amino}-4-O-methyl-D-erythro-pentitol;

   1,5-anhydro-2,3-dideoxy-3-{[(1 R,3S)-3-(2,2-difluoroethyl)-3-({(1 S,4S)-5-[6(trifluoromethyl)pyndin-2-yl]-2,5-diazabicyclo[2.2.1]hept-2yl}carbonyl)cyclopentyl]amino}-4-O-methyl-D-erythro-pentitol;

   1,5-anhydro-2_I3-dideoxy-3-{[(1R,3S)-3-(2,2-difluoroethyl)-3-({(1S,4S)-5-[6(trifluorornethyl)pyrazin-2-yl]-2,5-diazabicyclo[2.2.1]hept-2yl}carbonyl)cyclopentyl]amino}-4-O-methyl-D-erythro-pentitol;

   153

   1,5-anhydro-2,3-dideoxy-3-{[(1R,3S)-3-(2,2-difluoroethyl)-3-({(1S,4S)-5-[2(trifluoromethyl)pyrimidin-4-yl]-2,5-diazabicyclo[2.2.1]hept-2yl}carbonyl)cyclopentyl]amino}-4-O-methyl-D-erythro-pentitol;

   1,5-anhydro-2,3-dideoxy-3-{[(1R,3S)-3-(2,2-difluoroethyl)-3-({(1S,4S)-5-[5(trifluoromethyl)pyridazin-3-yl]-2,5-diazabicyclo[2.2.1]hept-2yl}carbonyl)cyclopentyl]amino}-4-O-methyl-D-erythro-pentitol;

   1,5-anhydro-2,3-dideoxy-4-O-methyl-3-{[(1 R,3S )-3-(2,2,2-trifluoroethyl)-3({(1S,4S)-5-[6-(trifluoromethyl)pyrimidin-4-yl]-2,5-diazabicyclo[2.2.1]hept-2yl}carbonyl)cyclopentyl]amino}-D-erythro-pentitol;

   1,5-anhydro-2,3-dideoxy-4-O-methyl-3-{[(1R,3S)-3-(2₁2,2-trifluoroethyl)-3({(1S,4S)-5-[4-(trifluoromethyl)pyridin-2-yl]-2,5-diazabicyclo[2.2.1]hept-2yl}carbonyl)cyclopentyl]amino}-D-erythro-pentitol;

   ï,5-anhydro-2,3-dideoxy-4-O-methyl-3-{[(1R,3S)-3-(2,2,2-tnfluoroethyl)-3({(1S,4S)-5-[2-(trifluoromethyl)pyridin-4-yl]-2,5-diazabicyclo[2.2.1]hept-2yl}carbonyl)cyclopentyl]amino}-D-erythro-pentitol;

   1,5-anhydro-2,3-dideoxy-4-O-methyl-3-{[(1R,3S)-3-(2,2,2-tnfluoroethyl)-3({(1S,4S)-5-[4-(trifluoromethyl)pyrimidin-2-yl]-2,5-diazabicyclo[2.2.1]hept-2yl}carbonyl)cyclopentyl]amino}-D-erythro-pentitol;

   1,5-anhydro-2,3-dideoxy-4-O-methyl-3-{[(1R,3S)-3-(2,2,2-trifluoroethyl)-3({(1S,4S)-5-[6-(trifluoromethyl)pyridin-2-yl]-2,5-diazabicyclo[2.2.1]hept-2yl}carbonyl)cyclopentyl]amino}-D-erythro-pentitol;

   154

   1₁5-anhydro-2,3-dideoxy-4-O-methyl-3-{[(1R,3S)-3-(2,2,2-trifluoroethyl)-3({(1S,4S)-5-[6-(trifluorOmethyl)pyrazin-2-yl]-2,5-diazabicyclo[2.2.1]hept-2yl}carbonyl)cyclopentyl]amîno}-D-erythro-pentitol;

   1₁5-anhydro-2,3-dîdeoxy-4-O-methyl-3-{[(1R_l3S)-3-(2,2,2-trifluoroethyl)-3({(1S,4S)-5-[2-(trifluoromethyl)pyrimidin-4-yl]-2,5-diazabicyclo[2.2.1]hept-2yl}carbonyl)cyclopentyl]amino}-D-erythro-pentitol;

   1,5-anhydro-2,3-dideoxy-4-O-methyl-3-{[(1R_l3S)-3-(2,2₁2-trifluoroethyl)-3({(1 S,4S)-5-[5-(trifIuoromethyl)pyridazin-3-yl]-2,5-diazabicyclo[2.2.1]hept-2yl}carbonyl)cyclopentyl]amino}-D-erythro-pentitol;

   1,5-anhydro-2,3-dideoxy-3-{[(1S,4S)-4-isopropyll-4-({(1S,4S)-5-[4(trifluoromethyl)pyridin-2-yl]-2,5-diazabicyclo[2.2.1]hept-2-yl}carbonyl)cyclopent2-en-1-yl]amino}-4-O-methyl-D-erythro-pentitol;

   1,5-anhydro-2,3-dideoxy-3-{[(1 S,4S)-4-isopropyll-4-({( 1 S,4S)-5-[4(trifluoromethyl)pyrimidin-2-yl]-2,5-diazabicyclo[2.2.1]hept-2yl}carbonyl)cyclopent-2-en-1-yl]amino}-4-O-methyl-D-erythro-pentitol;

   1_l5-anhydro-2,3-dideoxy-3-{[(1S,4S)-4-isopropyll-4-({(1S,4S)-5-[6(trifluoromethyl)pyridin-2-yl]-2,5-diazabicyclo[2.2.1]hept-2-yl}carbonyl)cyclopent2-en-1-yl]amino}-4-O-methyl-D-erythro-pentitol;

   1,5-anhydro-2,3-dideoxy-3-{[(1 S,4S)-4-isopropyll-4-({( 1 S,4S)-5-[6(trifluoromethyl)pyrazin-2-yl]-2,5-diazabicyclo[2.2.1]hept-2-yl}carbonyl)cyclopent2-en-1-yl]amino}-4-O-methyl-D-erythro-pentitol;

   1,5-anhydro-3-{[(1R,3S)-3-{[(1S,4S)-5-(terÎ-butoxycarbonyl)-2,5diazabicyclo[2.2.1]hept-2-yl]carbonyl}-3-ethylcyclopentyl]amino}-2,3-dideoxy-4-Omethyl-D-erythro-pentitol;

   155

   1,5-anhydro-3-{[(1R,3S)-3-{[(1S,4S)-5-(tert-butoxycarbonyl)-2,5diazabicyclo[2.2.1]hept-2-yl]carbonyl}-3-(2,2,2-tnfluoroethyl)cyclopentyl]amino}2,3-dideoxy-4-O-methyl-D-erythro-pentitol;

   1,5-Anhydro-2,3-dideoxy-3-{[(1 R, 3S )-3-(1-hydroxycyclobutyl)-3-({(1S,4S)-5-[6(trifluoromethyl)pyridin-2-yl]-2,5-diazabicyclo[2.2.1]hept-2yl}carbonyl)cyclopentyl]amino}-4-O-methyl-D-erythro-pentitol;

   1,5-Anhydro-2,3-dideoxy-3-{[(1 R,3S)-3-(1-hydroxycyclobutyl)-3-({(1S,4S)-5-[4(trifluoromethyl)pyridin-2-yl]-2,5-diazabicyclo[2.2.1]hept-2yl)carbonyl)cyclopentyl]amino}-4-O-methyl-D-erythro-pentitol;

   1,5-Anhydro-2,3-dideoxy-3-{[(1 R,3S)-3-(1-hydroxycyclobutyl)-3-({(1S,4S)-5-[4(trifluoromethyl)pyrimidin-2-yl]-2,5-diazabicydo[2.2.1]hept-2yl}carbonyl)cyclopentyl]amino}-4-O-methyl-D-erythro-pentitol;

   1,5-Anhydro-2,3-dideoxy-3-{[(1R,3S)-3-(1-hydroxycydobutyl)-3-({(1S,4S)-5-[2(trifluoromethyl)pyrimidin-4-yl]-2,5-diazabicydo[2.2.1]hept-2yl)carbonyl)cyclopentyl]amino}-4-O-methyl-D-erythro-pentitol

   1,5-anhydro-2,3-dideoxy-3-{[(1 R,3S )-3-( 1-hydroxycydobutyl)-3-({( 1S,4S)-5-[6(trifluoromethyl)pyrimidin-4-yl]-2,5-diazabicyclo[2.2.1]hept-2yl}carbonyl)cyclopentyl]amino}-4-O-methyl-D-erythro-pentitol;

   1,5-Anhydro-2,3-dideoxy-3-{[(1R,3S)-3-(1-hydroxycyclobutyl)-3-({(1S,4S)-5-[6(trifluoromethyl)pyrazin-2-yl]-2,5-diazabicyclo[2.2.1 ]hept-2yl}carbonyl)cyclopentyl]amino}-4-O-methyl-D-erythro-pentitol;

   156

   1,5-An hyd ro-2,3-dideoxy-3-{[( 1R, 3S )-3-( 1 -hyd roxycyclobutyl )-3-( {( 1S ,4S )-5-[2(trifluoromethyl)pyridin-4-yl]-2,5-diazabicyclo[2.2.1 ]hept-2yl}carbonyl)cyclopentyl]amino}-4-O-methyl-D-erythro-pentitol;

   1,5-An hyd ro-2,3-dideoxy-3-{[3-( 1 -hyd roxy-1 -m ethyl ethyl )-3-({5-[4(trifluoromethyl)pyridin-2-yl]-2,5-diazabicyclo[2.2.1]hept-2yQcarbonyl )cyclopentyl]amino)-4-O-methyl pentitol ;

   1,5-Anhydro-2,3-dideoxy-3-{[3-(1-hydroxy-1-methylethyl)-3-({5-[6(trifluoromethyl)pyrimidin-4-yl]-2,5-diazabicyclo[2,2.1]hept-2yl}carbonyl)cyclopentyl]amino)-4-O-methylpentitol;

   1,5-Anhydro-2,3-dideoxy-3-{[3-(1 -hydroxy-1 -methyl ethyl )-3-({5-[4(trifluoromethyl)pyrimidin-2-yl]-2,5-diazabicyclo[2.2.1]hept-2yl}carbonyl)cyclopentyl]amino}-4-O-methylpentitol;

   1,5-Anhydro-2,3-dideoxy-3-{[3-(1 -hydroxy-1 -methylethyl)-3-({5-[5(trifluoromethyl)pyridazin-3-yl]-2,5-diazabicyclo[2.2.1]hept-2yl}carbonyl)cyclopentyl]amino)-4-O-methylpentitol;

   1,5-Anhydro-2,3-dideoxy-3-{[3-(1-hydroxy-1-methylethyl)-3-({5-[2(trifluoromethyl)pyri midi n-4-yl]-2,5-diazabicyclo[2.2.1] hept-2yl}carbonyl)cyclopentyl]amino)-4-O-methylpentitol;

   1,5-Anhydro-2,3-dideoxy-3-{[3-(1-hydroxy-1-methylethyl)-3-({5-[6(trifluoromethyl)pyridin-2-yl]-2,5-diazabicyclo[2,2.1]hept-2yl}carbonyl)cyclopentyl]amino}-4-O-methylpentitol;

   1,5-Anhydro-2,3-dideoxy-3-{[3-(1 -hydroxy-1 -methylethyl)-3-({5-[2(trifluoromethyl)pyridin-4-yl]-2,5-diazabicyclo[2.2.1]hept-2yl}carbonyl)cyclopentyl]amino}-4-O-methylpentitol; and

   157

   1,5-Anhydro-2,3-dideoxy-3-{[3-(1’hydroxy-1-methylethyl)-3-({5-[6(trifluoromethyl)pyrazin-2-yl]-2,5-diazabicyclo[2.2.1]hept-2yl}carbonyl)cyclopentyl]amino}-4-O-methylpentitol.
8. 10. A compound of formula:

   10 pharmaceutically acceptable sait thereof.

   pharmaceutically acceptable sait thereof.
9. 12. A composition comprising a compound of claim 2, or a pharmaceutically acceptable sait therof, and a carrier.

   158
10. 13. Use of a compound of claim 2, or a pharmaceutically acceptable sait thereof, in the manufacture of a médicament for treating a disease associated with expression or activity of a chemokine receptor in a patient.

    5
11. 14. The use of claim 13 wherein said chemokine receptor is CCR2 or CCR5.
12. 15. The use of claim 13 wherein said disease is rheumatoid arthritis, atherosclerosis, lupus, multiple sclerosis, pain, transplant rejection, diabètes, liver fibrosis, viral disease, cancer, asthma, seasonal and perennial allergie rhinitis, 10 sinusitis, conjunctivitis, age-related macular degeneration, food allergy, scombroid poisoning, psoriasis, undifferentiated spondyloarthropy, goût, urticaria, pruritus, eczema, inflammatory bowel disease, thrombotic disease, otitis media, fibrosis, liver cirrhosis, cardiac disease, Alzheimer's disease, sepsis, restenosis, Crohn's disease, ulcerative colitis, irritable bowel syndrome, hypersensitivity lung 15 diseases, drug-induced pulmonary fibrosis, chronic obstructive pulmonary disease, arthritis, nephritis, atopie dermatitis, stroke, acute nerve injury, sarcoidosis, hepatitis, endometriosis, neuropathie pain, hypersensitivity pneumonitis, éosinophilie pneumonias, delayed-type hypersensitivity, interstitial lung disease, eye disorders or obesity.
13. 16. Use of a compound of any of daims 1-11, or a pharmaceutically acceptable sait thereof, in the manufacture of a médicament for the treatment of rheumatoid arthritis, atherosclerosis, lupus, multiple sclerosis, pain, transplant rejection, diabètes, liver fibrosis, viral disease, cancer, asthma, seasonal and 25 perennial allergie rhinitis, sinusitis, conjunctivitis, age-related macular degeneration, food allergy, scombroid poisoning, psoriasis, undifferentiated spondyloarthropy, goût, urticaria, pruritus, eczema, inflammatory bowel disease, thrombotic disease, otitis media, fibrosis, liver cirrhosis, cardiac disease, Alzheimer's disease, sepsis, restenosis, Crohn's disease, ulcerative colitis, 30 irritable bowel syndrome, hypersensitivity lung diseases, drug-induced pulmonary fibrosis, chronic obstructive pulmonary disease, arthritis, nephritis, atopie

    159 dermatitis, stroke, acute nerve injury, sarcoidosis, hepatitis, endometriosis, neuropathie pain, hypersensitivity pneumonitis, éosinophilie pneumonias, delayed-type hypersensitivity, interstitial lung disease, eye disorders or obesity.