OA16816A - Improved method for synthesizing pirfenidone - Google Patents
Improved method for synthesizing pirfenidone Download PDFInfo
- Publication number
- OA16816A OA16816A OA1201100459 OA16816A OA 16816 A OA16816 A OA 16816A OA 1201100459 OA1201100459 OA 1201100459 OA 16816 A OA16816 A OA 16816A
- Authority
- OA
- OAPI
- Prior art keywords
- pirfenidone
- solution
- weight
- molar ratio
- bromobenzene
- Prior art date
Links
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- 230000002194 synthesizing Effects 0.000 title claims abstract description 9
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Abstract
A process for synthesizing pirfenidone from bromobenzene having less than about 0.15% by weight dibromobenze is disclosed. Also disclosed are processes of synthesizing pirfenidone without using ethyl acetate or n-butanol, and pirfenidone having controlled levels of ethyl acetate, nbutanol, di(5-methyl-2-pyridinone)benzenes, and other impurities having specified retention times. Also disclosed are formulated dosage forms including the disclosed pirfenidone.
Description
This disclosure relates generally to methods for synthesizing pirfenidone. More specifically, this disclosure relates to a process for preparing pirfenidone using a bromobenzene reagent having less than about 0.15% by weight dibromobenzene and using copper(l) oxide as a catalyst, instead of a copper (I) or (II) halide.
Brief Description of the Related Art
Pirfenidone is a non-peptide synthetic molécule with a molecular weight of 185.23 daltons. Its chemical éléments are expressed as C^H^NO, and its structure is known. The synthesis of pirfenidone has been worked out. Pirfenidone is manufactured and being evaluated clinically as a broad-spectrum anti-fibrotic drug. Pirfenidone has anti-fibrotic properties via: decreased TNF-α expression, decreased PDGF expression, and decreased collagen expression. Several pirfenidone Investigational New Drug Applications (INDs) are currently on file with the U.S. Food and Drug Administration. Phase II human investigations hâve been initiated orcompleted for pulmonary fîbrosis, rénal glomerulosclerosis, and liver cirrhosis. There hâve been other Phase II studies that used pirfenidone to treat benign prostate hypertrophy, hypertrophie scarring (keloids), and rheumatoîd arthritis.
One important use of pirfenidone is known to be providing therapeutic benefits to patients suffering from fîbrosis conditions such as Hermansky-Pudlak Syndrome (HPS) associated pulmonary fîbrosis and idiopathic pulmonary fîbrosis (IPF). Pirfenidone demonstrates a pharmacologie ability to prevent or remove excessive scar tissue found in fîbrosis associated with injured tissues including that of lungs, skin, joints, kidneys, prostate glands, and livers. Published and unpublished basic and clinical research suggests that pirfenidone may safely slow or inhibit the progressive enlargement of fibrotic lésions, remove pre-existing fibrotic lésions, and prevent formation of new fibrotic lésions following tissue injuries.
It is understood that one mechanism by which pirfenidone exerts its therapeutic effects is by modulating cytokine actions. Pirfenidone is a potent inhibitor of fibrogenic cytokines and TNF-a. It is well documented that pirfenidone inhibits excessive biosynthesis or release of various fîbrogenic cytokines such as TGF-βΙ, bFGF, PDGF, and EGF. Zhang S et al., Australian New Eng. J. Ophthal., 26:S74-S76 (1998). Experimental reports also show that pirfenidone blocks the synthesis and release of excessive amounts of TNF-α from macrophages and other cells. Cain et al.. Int. J. Immunopharm., 20:685-695 (1998).
Pirfenidone has been studied in clinical trials for use in treatment of IPF. Thus, there is a need for a synthetic scheme that provides pirfenidone having sufficient purity as an active pharmaceutical ingrédient (API) and involves efficient and economical processes. Prior batches of pirfenidone were shown to hâve residual solvent traces of ethyl acetate (e.g., about 2 ppm) and butanol.
SUMMARY
Dîsclosed herein are methods of preparing pirfenidone. More specifically, dîsclosed herein is a method of synthesizing pirfenidone comprising admixing bromobenzene, 5-methyl-2-pyridone, cuprous oxide, and an organic solvent under conditions sufficient to form pirfenidone, wherein the bromobenzene comprises less than about 0.15% by weight dibromobenzene. The admixing can be performed under elevated températures, for example at least about 100°C. The organic solvent can comprise dimethyl formamide.
In some embodiments, the method further comprises washing the pirfenidone with a saline solution. The saline solution can comprise about 10 wt% to about 15 wt% sodium chloride. In various embodiments, the method further comprises extracting the pirfenidone with an extracting solvent, The extracting solvent can comprise toluene.
In various embodiments, the method further comprises admixing a base with the bromobenzene, 5-methyl-2-pyridone, cuprous oxide, and organic solvent. The base can be an inorganic base. In a spécifie embodiment, the inorganic base comprises a carbonate, and more specifically, potassium carbonate.
In some embodiments, the method further comprises crystallizing the pirfenidone from a solvent mixture comprising heptanes and toluene to form purified pirfenidone. The method can also furthercomprise recrystallizing the purified pirfenidone by dissolving at least a portion ofthe purified pirfenidone in an acidic aqueous solution at an elevated température to form a pirfenidone solution; adding a basic solution to the pirfenidone solution until the pH is at least about 11; and cooling the basic pirfenidone solution to a température below about 20°C to form recrystallized pirfenidone. In some cases, the elevated température is at least about 40°C. In various cases, the acidic aqueous solution comprises hydrochloric acid, in some cases, the basic solution comprises sodium hydroxide. In various cases, the basic pirfenidone solution is cooled to a température below about 10°C. In some cases, the purifying of the pirfenidone is performed in the absence of ethyl acetate and butanol. In a spécifie case, the pirfenidone prepared by the methods disclosed herein has a purity of at least 98% by weight or molar ratio and is essentially free of or free of ethyl acetate and butanol, where essentially free of describes a product where neither ethyl acetate nor butanol is intentionally added during the synthetic processes, as described herein. A pirfenidone composition essentially free of ethyl acetate and/or butanol can permit the presence of trace amounts of ethyl acetate and/or butanol which are carry-over impurities, e.g. présent in the reagents or starting materials used in a synthetic process such as one described herein. The pirfenidone prepared can hâve a purity of at least 99%, and more preferably, at least 99.9% by weight or molar ratio.
In another aspect, disclosed herein is pirfenidone having less than about 0.1 % by weight or molar ratio of a di(5-methyl-2-pyridone)benzene impurity, and preferably less than about 0.05% by weight or molar ratio of a di(5-methyl-2-pyridone)benzene impurity.
In yet another aspect, disclosed herein is pirfenidone having less than about 0.1% by weight or molar ratio of an impurity which elutes at a relative rétention time of about 1.95 compared to the rétention time of pirfenidone, when analyzed by liquid chromatography. Preferably, the pirfenidone has less than about 0.05% by weight or molar ratio of the impurity with the relative rétention time of 1.95.
In still another aspect, disclosed herein is pirfenidone having less than about 0.1% by weight or molar ratio of an impurity which elutes at a relative rétention time of about 1.24 compared to the rétention time of pirfenidone, when analyzed by liquid chromatography, Preferably, the pirfenidone has less than about 0.05% by weight or molar ratio of the impurity with the relative rétention time of 1.24.
In another aspect, pharmaceutical compositions comprising pirfenidone as disclosed herein and a pharmaceutically acceptable excipient are described.
DETAILED DESCRIPTION
Disclosed herein is an improved process for preparing pirfenidone. The process involves using a cuprous oxide catalyst to couple 5-methyl-2-pyridone and bromobenzene in an organic solvent. Without intending to be limited by any particular theory, it is believed that the purity of the bromobenzene is important, as amounts of a dibromobenzene impurity in the bromobenzene can lead to dimer-type byproducts, which can complicate the purification of the resulting pirfenidone. These dimer-type byproducts cannot be in a product intended as to be marketed as an active pharmaceutical ingrédient (API), and they are difficult to remove from the intended pirfenidone product. Thus, the bromobenzene used in the disclosed processes preferably hâve an amount of dibromobenzene of less than about 0.15% by weight or molar ratio, and more preferably less than about 0.1% by weight or molar ratio or less than 0.05% by weight or molar ratio. The synthesis of pirfenidone is shown in Scheme 1, below.
Scheme 1
purified pirfenidone
As used throughout this disclosure, impurities and purity of compounds are reported as a percentage (%). Unless indicated otherwise for spécifie cases, this percentage can be assessed based upon the weight of the sample or composition (e.g., a wt%), or based upon a molar ratio. Molar ratios (e.g., molar percentages) can be measured using these chromatographie techniques, such as high pressure/high performance liquid chromatography (HPLC), gas chromatography (GC), or capillary electrophoresis (CE). Molar ratios are molecular ratios of the specified compound to the total compounds présent.
The bromobenzene preferably is pure monobromobenzene. The bromobenzene preferably is free of 1,4-dibromobenzene. The bromobenzene preferably is free of ail dibromobenzenes (i.e., 1,2-dibromobenzene, 1,3-dibromobenzene, and 1,4-dibromobenzene). The bromobenzene preferably is free of tribromobenzenes (i.e., 1,2,3-tribromobenzene, 1,2,4-tribromobenzene, and 1,3,5-tribromobenzene). The bromobenzene preferably is free of tetrabromobenzenes (i.e., 1,2,3,4-tetrabromobenzene, 1,2,3,5-tetrachlorobenzene, and 1,2,4,5-tetrabromobenzene). The bromobenzene preferably is free of pentabromobenzene and hexabromobenzene.
The organic solvent of the coupling reaction can be any compatible organic solvent, such as an aprotic polar solvent. Non-limiting examples include tetrahydrofuran, diethyl ether, dimethyl formamide, dimethylsulfoxide, dichloromethane, dimethylsulfoxide, sulfolane, and mixtures thereof. in a spécifie preferred embodiment, the organic solvent comprises dimethyl formamide. Choice of the appropriate solvent can dépend upon the température at which the reaction is run. A solvent having a boiling point above or around that of the reaction température is preferred.
The reaction can be performed at elevated températures. An elevated température is any température above room température (about 25eC), and can be at least about 50eC, at least about 70°C, at least about 75’C, at least about 80Ό, at least about 85°C, at least about 90°C, at least about 95°C, at least about 100°C, at least about 105°C, at least about 110°C, at least about 115°C, at least about 120°C, at least about 125°C, at least about at least about130°C, or at least about 135°C, for example.
The reaction can be performed in the presence of a base. In some cases, the base can be an inorganic base. Inorganic bases include, but are not limited to, lithium hydroxide, potassium hydroxide, sodium hydroxide, sodium carbonate, potassium carbonate, sodium bicarbonate, potassium bicarbonate, and mixtures thereof. In one embodiment, the base comprises potassium carbonate.
Upon formation of the pirfenidone from the coupling reaction, the pirfenidone can optionally be washed with a saline solution. This washing step can remove salts used or formed during the coupling reaction. A saline solution can be a sodium chloride solution, for example a saturated brine solution. A high concentration sodium chloride solution provides low yield loss of pirfenidone in the aqueous layer, but a low concentration sodium chloride solution provides better compatibility with dimethyl formamide. Thus, an optimal sodium chloride solution concentration is high enough to minimize loss of pirfenidone in the aqueous layer, but low enough to allow a maximum amount of dimethyl formamide in the aqueous layer. Thus, in some embodiments, the sodium chloride solution is in a range of about 10wt% to about 20wt% sodium chloride, based on the weight of the solution.
The pirfenidone can additionally or alternative^ be extracted with an organic solvent to remove impurities, such as residual organic solvent from the coupling reaction (e.g., dimethyl formamide). Examples of an extracting organic solvent include, but are not limited to, toluene, diethyl ether, tetrahydrofuran, methylene chloride, and mixtures thereof. In a spécifie embodiment, the pirfenidone is extracted with toluene. Extraction with toluene can allow for residual dimethyl formamide to remain in the aqueous layer.
The pirfenidone can be precipitated to form a more purified form of pirfenidone. Crude pirfenidone can be dissolved in a minimum amount of toluene and heptanes and heated to,
e.g., about 50°C to about 100°C. The solution is slowly cooled to about -5°C to about 5°C to allow précipitation of the pirfenidone. The resulting solid pirfenidone precipitate can be collected via filtration and dried.
The pirfenidone can additionally or alternatively be crystallized to form a more purified pirfenidone. For crystallization, the pirfenidone can be dissolved or partially dissolved in an acidic solution at an elevated température, such as at least about 35°C, at least about 40°C, or about 40°C to about 50°C. The acidic solution can comprise any compatible inorganic or organic acid. The acidic solution preferably comprises an inorganic acid. Non-limiting examples of contemplated inorganic acids include sulfuric acid, hydrochloric acid, hydrobromic acid, nitric acid, phosphoric acid, and mixtures thereof. In a spécifie embodiment, the acidic solution comprises hydrochloric acid.
The resulting acidic pirfenidone solution can then be treated with addition of a basic solution until the pH of the pirfenidone solution is at least about 11. The basic solution can comprise any compatible inorganic or organic base. The basic solution is preferably an inorganic base. Non-limiting examples of contemplated inorganic bases include lithium hydroxide, potassium hydroxide, sodium hydroxide, sodium carbonate, potassium carbonate, sodium bicarbonate, potassium bicarbonate, and mixtures thereof. In a spécifie embodiment, the basic solution comprises sodium hydroxide.
The basic pirfenidone solution can then be cooled slowly to less than about 10°C, or about 0°C to about 9°C. The resulting crystallized pirfenidone can then be collected via filtration and dried.
For pirfenidone used in clinical trials and as an API, it is important that the trace solvent levels be minimized, for example for regulatory requirements. Thus, in one aspect, the pirfenidone is essentially free of or free of ethyl acetate or butanol, for example from having been prepared without the use of ethyl acetate and/or butanol in any step. In some cases, the pirfenidone has less than about 30 ppm toluene, heptanes, or both, and preferably has less than about 20 ppm toluene, heptanes, or both. The prepared pirfenidone has a purity of at least 98% by weight or molar ratio, and can hâve a purity of preferably at least 99% by weight or molar ratio or at least 99.5% by weight or molar ratio or at least 99.6% by weight or molar ratio, at least 99.7% by weight or molar ratio, at least 99.8% by weight or molar ratio, or at least 99.9% by weight or molar ratio.
Pirfenidone according to the dîsclosure herein, for example prepared using a method disclosed herein, can additionally or alternatively hâve less than about 0.1% by weight or molar ratio, less than about 0.05% by weight or molar ratio, less than about 0.04% by weight or molar ratio, less than about 0.03% by weight or molar ratio, less than about 0.02% by weight or molar ratio, or less than about 0.01 % by weight or molar ratio of a di(5-methyl-2-pyridlnone)benzene impurity. Examples of such di(5-methyl-2-pyridinone)benzene împurities include one or more of
The pirfenidone can additionally or alternatively hâve less than about 0.1% by weight or molar ratio, less than about 0.05% by weight or molar ratio, less than about 0.04% by weight or molar ratio, less than about 0.03% by weight or molar ratio, less than about 0.02% by weight or molar ratio, or less than about 0.01 % by weight or molar ratio of an impurity that has a relative rétention time (RRT) of about 1.95 compared to the rétention time of pirfenidone, when analyzed by liquid chromatography. The pirfenidone can additionally or alternatively hâve less than about 0.1% by weight or molar ratio, less than about 0.05% by weight or molar ratio, less than about 0.04% by weight or molar ratio, less than about 0.03% by weight or molar ratio, less than about 0.02% by weight or molar ratio, or less than about 0.01% by weight or molar ratio of an impurity that has a relative rétention time (RRT) of about 1.24 compared to the rétention time of pirfenidone, when analyzed by liquid chromatography.
An exemplary method for liquid chromatography (LC) analysis of pirfenidone is using a HewlettPackard 11000 Liquid Chromatograph, equipped with a UV detector operating at 220 nm and 310 nm, and a ZORBAX SB-Aq C-18, 5.0 pm, 250 mm x 4.6 mm column. The mobile phases are Mobile Phase A (MPA) (200 pL phosphoric acid in 1000 mL water) and Mobile Phase B (MPB) (acetonitrile), which are pumped through the column at a flow rate of 1.0 mL/min and column température of 35°C. The mixture of MPA and MPB was a gradient profile over the course of the 25 minute run, as follows:
Time (Minutes) | % MPA | % MPB |
0 | 80 | 20 |
20 | 10 | 90 |
21 | 80 | 20 |
25 | 80 | 20 |
Pharmaceutical Compositions
While it is possible for the pirfenidone described herein to be administered alone, it may be préférable to formulate pirfenidone as pharmaceutical compositions. In particular, the pharmaceutical compositions can be useful for treating or preventing inflammatory conditions, e.g., conditions associated with p38 activity or cytokine activity or any combination thereof. A pharmaceutical composition is any composition that may be administered in vitro or in vivo or both to a subject in order to treat or ameliorate a condition. In a preferred embodiment, a pharmaceutical composition may be administered in vivo. A subject may include one or more cells or tissues, or organisme. A preferred subject is a mammal. A mammal includes any mammal, such as by way of non-limiting example, cattle, pigs, sheep, goats, horses, camels, buffalo, cats, dogs, rats, mice, and humans. A highly preferred subject mammal is a human.
In an embodiment, the pharmaceutical compositions may be formulated with pharmaceutically acceptable excipients such as carriers, solvents, stabilizers, adjuvants, diluents, etc., depending upon the particular mode of administration and dosage form. The pharmaceutical compositions should generally be formulated to achieve a physiologically compatible pH, and may range from a pH of about 3 to a pH of about 11, preferably about pH 3 to about pH 7, depending on the formulation and route of administration. In alternative embodiments, it may be preferred that the pH Is adjusted to a range from about pH 5.0 to about pH 8. More particularly, the pharmaceutical compositions may comprise a therapeuticaliy or prophylactically effective amount of at least one compound as described herein, together with one or more pharmaceutically acceptable excipients. Optionally, the pharmaceutical compositions may include a second active ingrédient useful in the treatment or prévention of bacterial infection (e.g., a preservative, such as anti-bacterial or anti-microbial agents).
Formulations, e.g., for parentéral or oral administration, are most typically solids, liquid solutions, émulsions or suspensions, while inhalable formulations for pulmonary administration are generally liquids or powders, with powder formulations being generally preferred. A preferred pharmaceutical composition may also be formulated as a lyophilized solid that is reconstituted with a physiologically compatible solvent prior to administration. Alternative pharmaceutical compositions may be formulated as syrups, creams, ointments, tablets, and the like.
The term “pharmaceutically acceptable excipient” refers to an excipient for administration of a pharmaceutical agent, such as the compounds described herein. The term refers to any pharmaceutical excipient that may be administered without undue toxicity.
Pharmaceutically acceptable excipients are determined in part by the particular composition being administered, as well as by the particular method used to admrnister the composition. Accordingly, there exists a wide variety of suitable formulations of pharmaceutical compositions (see, e.g., Remington's Pharmaceutical Sciences).
Suitable excipients may be carrier molécules that inciude large, slowly metabolized macromolecules such as proteins, polysaccharides, polylactic acids, polyglycolic acids, polymeric amino acids, amino acid copolymers, and inactive virus particles. Other exemplary excipients inciude antioxidants (e.g., ascorbic acid), chelatîng agents (e.g., EDTA), carbohydrates (e.g., dextrin, hydroxyalkylcellulose, and/or hydroxyalkylmethylcellulose), stearic acid, liquids (e.g., oils, water, saline, glycerol and/or éthanol) wetting or emulsifying agents, pH buffering substances, and the like. Liposomes are also included within the définition of pharmaceutically acceptable excipients.
The pharmaceutical compositions described herein may be formulated in any form suitable for an intended method of administration. When intended for oral use for example, tablets, troches, lozenges, aqueous or oil suspensions, non-aqueous solutions, dispersible powders or granules (including micronized particles or nanoparticles), émulsions, hard or soft capsules, syrups or élixirs may be prepared. Compositions intended for oral use may be prepared according to any method known to the art for the manufacture of pharmaceutical compositions, and such compositions may contain one or more agents including sweetening agents, flavoring agents, coloring agents and preserving agents, in order to provide a palatable préparation. Pharmaceutically acceptable excipients particularly suitable for use in conjunction with tablets inciude, for example, inert diluents, such as celluloses, calcium or sodium carbonate, lactose, calcium or sodium phosphate; disintegrating agents, such as cross-linked povidone, maize starch, or algînic acid; binding agents, such as povidone, starch, gelatin or acacia; and lubricating agents, such as magnésium stéarate, stearic acid or talc.
Tablets may be uncoated or may be coated by known techniques including microencapsulation to delay disintegration and adsorption in the gastrointestinal tract and thereby provide a sustained action over a longer period. For example, a time delay material such as glyceryl monostearate or glyceryl distearate alone or with a wax may be employed as a coating or as a matrix.
Formulations for oral use may be also presented as hard gelatin capsules wherein the active ingrédient is mixed with an inert solid diluent, for example celluloses, lactose, calcium phosphate or kaolin, or as soft gelatin capsules wherein the active ingrédient is mixed with nonaqueous or oil medium, such as glycerin, propylene glycol, polyethylene glycol, peanut oil, liquid paraffin or olive oil.
In another embodiment, pharmaceutical compositions may be formulated as suspensions comprising a compound of the embodiments in admixture with at least one pharmaceutically acceptable excipient suitable for the manufacture of a suspension.
In yet another embodiment, pharmaceutical compositions may be formulated as dispersible powders and granules suitable for préparation of a suspension by the addition of suitable excipients.
Excipients suitable for use in connection with suspensions include suspending agents (e.g., sodium carboxymethylcellulose, methylcellulose, hydroxypropyl methylcellulose, sodium alginate, polyvinylpyrrolidone, gum tragacanth, gum acacia); dispersing orwetting agents (e.g., a naturally occurring phosphatide (e.g., lecithin), a condensation product of an alkylene oxide with a fatty acid (e.g., polyoxyethylene stéarate), a condensation product of ethylene oxide with a long chain aliphatic alcohol (e.g., heptadecaethyleneoxycethanol), a condensation product of ethylene oxide with a partial ester derived from a fatty acid and a hexitol anhydride (e.g., polyoxyethylene sorbitan monooleate)); and thickening agents (e.g., carbomer, beeswax, hard paraffin or cetyl alcohol). The suspensions may also contain one or more preservatives (e.g., acetic acid, methyl or n-propyl p-hydroxy-benzoate); one or more coloring agents; one or more flavoring agents; and one or more sweetening agents such as sucrose or saccharin.
The pharmaceutical compositions may also be in the form of oil-in water émulsions. The oily phase may be a vegetable oil, such as olive oil or arachis oil, a minerai oil, such as liquid paraffin, or a mixture of these. Suitable emulsifying agents include naturally-occurring gums, such as gum acacia and gum tragacanth; naturally occurring phosphatides, such as soybean lecithin, esters or partial esters derived from fatty acids; hexitol anhydrides, such as sorbitan monooleate; and condensation products of these partial esters with ethylene oxide, such as polyoxyethylene sorbitan monooleate. The émulsion may also contain sweetening and flavoring agents. Syrups and élixirs may be formulated with sweetening agents, such as glycerol, sorbitol or sucrose. Such formulations may also contain a demulcent, a preservative, a flavoring or a coloring agent.
I0
Additionally, the pharmaceutical compositions may be in the form of a stérile injectable préparation, such as a stérile injectable aqueous émulsion or oleaginous suspension, This émulsion or suspension may be formulated by a person of ordinary skill in the art using those suitable dispersing or wetting agents and suspending agents, including those mentloned above. The stérile injectable préparation may also be a stérile injectable solution or suspension in a non-toxic parenterally acceptable diluent or solvent, such as a solution in 1,2-propane-diol.
The stérile injectable préparation may also be prepared as a lyophilized powder. Among the acceptable vehicles and solvents that may be employed are water, Ringeris solution, and isotonie sodium chloride solution, ln addition, stérile fixed oils may be employed as a solvent or suspending medium. For this purpose any bland fixed oil may be employed including synthetic mono- or diglycerides. ln addition, fatty acids (e.g., oleic acid) may likewise be used in the préparation of injectables.
To obtain a stable water-soluble dose form of a pharmaceutical composition, a pharmaceutically acceptable sait of a compound described herein may be dissolved in an aqueous solution of an organic or inorganic acid, such as 0.3 M solution of succinic acid, or more preferably, cïtric acid. If a soluble sait form is not available, the compound may be dissolved in a suitable co-solvent or combination of co-solvents. Examples of suitable cosolvents include alcohol, propylene glycol, polyethylene glycol 300, polysorbate 80, glycerin and the like in concentrations ranging from about 0 to about 60% of the total volume. In one embodiment, the active compound is dissolved in DMSO and diluted with water.
The pharmaceutical composition may also be in the form of a solution of a sait form of the active ingrédient in an appropriate aqueous vehicle, such as water or isotonie saline or dextrose solution. Also contemplated are compounds which hâve been modified by substitutions or additions of chemical or biochemical moieties which make them more suitable for delivery (e.g., increase solubility, bioactivity, palatability, decrease adverse reactions, etc.), for example by estérification, glycosylation, PEGylation, etc.
ln a preferred embodiment, pirfenidone described herein may be formulated for oral administration in a lipid-based formulation suitable for low solubility compounds. Lipid-based formulations can generally enhance the oral bioavailability of such compounds.
As such, a preferred pharmaceutical composition comprises a therapeutîcally or prophylactically effective amount of pirfenidone described herein, together with at least one pharmaceutically acceptable excipient selected from the group consisting of medium chain fatty acids and propylene glycol esters thereof (e.g., propylene glycol esters of edible fatty acids, such as caprylic and caprîc fatty acids) and pharmaceutically acceptable surfactants, such as polyoxyl 40 hydrogenated castor oil.
ln an alternative preferred embodiment, cyclodextrins may be added as aqueous solubility enhancers. Preferred cyclodextrins include hydroxypropyl, hydroxyethyl, glucosyl, maltosyl and maltotriosyl dérivatives of α-, β-, and y-cyclodextrin. A partîcularly preferred cyclodextrin solubility enhancer is hydroxypropyl-o-cyclodextrin (BPBC), which may be added to any of the above-described compositions to further improve the aqueous solubility characteristics of the compounds of the embodiments. ln one embodiment, the composition comprises about 0.1% to about 20% hydroxypropyl-o-cyclodextrin, more preferably about 1% to about 15% hydroxypropyl-o-cyclodextrin, and even more preferably from about 2.5% to about 10% hydroxypropyl-o-cyclodextrin. The amount of solubility enhancer employed will dépend on the amount of the compound of the invention in the composition.
A pharmaceutical composition contains a total amount of the active ingredient(s) sufficient to achieve an intended therapeutic effect when used with a suitable dosing regimen. More specifically, in some embodiments, the pharmaceutical composition contains a therapeutically effective amount. The total amounts of pirfenidone that may be combined with the carrier materials to produce a unitary dosing form will vary depending upon the host treated and the particular mode of administration. Preferably, the compositions are formulated in view of contemplated dosing regimens so that a dose of between 0.01 to 100 mg/kg body weight/day of pirfenidone is administered to a patient receiving the compositions. The total daily dose may be provided in divided daily doses (e.g. two times per day, three times per day, four times per day), and administered as multiple dosage forms containing sub-therapeutic dosage amounts (e.g., 267 mg per dosage form, administered as three dosage forms taken three times per day for a total of nine dosage forms administered, e.g. 2403 mg/day pirfenidone).
EXAMPLES
Coupling of Bromobenzene and 5-Methyl-2-pyridone
5-Methyl-2-pyridone (1.0 équivalents), potassium carbonate (1.2 équivalents), copper(l) oxide (0.05 équivalents), bromobenzene (1.8 équivalents, with a purity of at least 98%, preferably at least 99%, or at least 99.8%), and dimethyl formamide (2.0 volume équivalents) were charged into an inert reactor. This mixture was heated to 125°C for about 18 hours. A sample was collected and analyzed for reaction completion. If reaction completion was not satisfactory, the reaction was maintained at 125°C for an additional 2 hours. The reaction mixture was then cooled to 25°C to form a slurry.
The resulting slurry was filtered in a Nutsche filter in order to remove salts. The filter cake was rinsed twice with toluene. The mother liquor and process liquor were collected in Vessel (A). A sodium chloride solution (15%) was charged into the product solution. The pH was adjusted to greater than or equal to 11.5 using a 32% sodium hydroxide solution. The mixture was then agitated. After agitation was stopped, the mixture was allowed to settle for at least 30 minutes to allow the two phases to separate. The organic layer was separated and the aqueous layer was extracted with toluene. The toluene extraction was added to the organic layer. The combined organics were then washed with a 15% sodium chloride solution and agitated for at least 15 minutes. The agitation was stopped and the layers were allowed to settle for at least 30 minutes. The organic layer was separated from the aqueous layer, and then carbon treated by flowing it through Zêta Carbon filters for 2 hours at 20-25’C. The carbon treated solution was then concentrated under vacuum to remove ali water and much of the toluene.
Heptanes were then added to the concentrated solution, and it was heated to about 80°C. The solution was slowly cooled to about 0°C over at least 7 hours. The pirfenidone precipitated out of the solution, was collected by filtration and dried, using a Nutsche filter/drier. The pirfenidone cake was washed twice with a mixture of toluene and heptanes (at 0°C), then vacuum dried at a température of about 42°C. The crude pirfenidone was formed in about 85% yield.
Crystallization of Pirfenidone
Pirfenidone, a 32% hydrochloride solution, and deionized water were charged in an inert reactor. The mixture was heated to about 45°C, then a 32% sodium hydroxide solution was titrated into the mixture until the pH was at least 11. The température of the mixture was maintained at about 45°C during the titration. Upon reaching the pH of at least 11, the mixture was then cooled slowly to 5°C, over the course of at least 2 hours. The pirfenidone crystallized from this cooled solution and was isolated in a Nutsche filter/drier. The pirfenidone cake was washed twice with deionized water (at S’C). The pirfenidone was then vacuum dried in the filter/drier at a température of about 45°C. The pirfenidone was also milled through a loop mill in order to reduce the particle size to less than 150 pm.
The resulting pirfenidone was then analyzed and the only residual solvents observed were toluene and heptanes at about 10 to 13 ppm. No ethyl acetate or butanol was detected in the pirfenidone. The amount of bis-conjugate in the purified pirfenidone was 0.03% or less. Ail impurities of the purified pirfenidone were less than about 0.05%.
The foregoing description is given for ciearness of understanding only, and no unnecessary limitations should be understood therefrom, as modifications within the scope of the invention may be apparent to those having ordinary skill in the art.
Throughout the spécification, where methods are described as including steps, components, or materials, it is contemplated that the compositions can also consist essentially of, or consist of, any combination of the recited steps, components or materials, unless described otherwise. The practice of a method disclosed herein, and individual steps thereof, can be performed manually and/or with the aid of electronic equipment. Although processes hâve been described with reference to particular embodiments, a person of ordinary skill in the art will readily appreciate that other ways of performing the acts associated with the methods may be used. For example, the order of various of the steps may be changed without departing from the scope or spirit of the method, unless described otherwise. In addition, some of the individual steps can be combined, omitted, or further subdivided into additional steps.
Ali patents, publications and référencés cited herein are hereby fully incorporated by reference.
In case of conflict between the présent disclosure and incorporated patents, publications and references, the présent disclosure should control.
Claims (25)
1. A method of synthesizing pirfenidone comprising admixing bromobenzene, 5-methyl-2-pyridone, cuprous oxide, and an organic solvent under conditions sufficient to form pirfenidone, wherein the bromobenzene comprises less than about 0.15% by weight or molar ratio dibromobenzene.
2. The method of claim 1, further comprising washing the pirfenidone with a saline solution.
3. The method of claim 2, wherein the saline solution comprises sodium chlorîde in a range of about 10 wt% to about 15 wt% based on the total weight of the solution.
4. The method of any one of daims 1-3, further comprising extracting the pirfenidone with an extracting organic solvent.
5. The method of claim 4, wherein the extracting organic solvent comprises toluene.
6. The method of any one of daims 1-5, wherein the admixing is performed under elevated températures.
7. The method of claim 6, wherein the température is at least about 100qC.
8. The method of any one of daims 1-7, further comprising admixing a base with the bromobenzene, 5-methyl-2-pyridone, cuprous oxide, and organic solvent.
9. The method of daim 8, wherein the base is an inorganic base.
10. The method of claim 9, wherein the inorganic base comprises a carbonate.
11. The method of claim 10, wherein the carbonate comprises potassium carbonate.
12. The method of any one of daims 1-11, wherein the organic solvent comprises dimethyl formamide.
13. The method of any one of daims 1 -12, further comprising crystalItzing the pirfenidone from a solvent mixture comprising heptanes and toluene to form purified pirfenidone.
14. The method of claim 13, further comprising recrystallizing the purified pirfenidone by dissolving at least a portion of the purified pirfenidone in an acidic aqueous solution at an elevated température to form a pirfenidone solution;
adding a basic solution to the pirfenidone solution until the pH is at least about 11; and cooling the basic pirfenidone solution to a température below about 20°C to form recrystallized pirfenidone.
15. The method of claim 14, wherein the elevated température is at least about 40°C.
16. The method of claim 14 or 15, wherein the acidic aqueous solution comprises hydrochloric acid.
17. The method of any one of claims 14-16, wherein the basic solution comprises sodium hydroxide.
18. The method of any one of claims 14-17, comprising cooling the basic pirfenidone solution to a température below about 10°C.
19. The method of any one of claims 13-18, comprising perfomning the purifying in the absence of ethyl acetate and butanol.
20. Pirfenidone prepared by the method of claim 19, having a purity of at least 98% by weight or molar ratio and essentially free of ethyl acetate and butanol.
21. The pirfenidone of claim 20, having a purity of at least 99% by weight or molar ratio.
22. The pirfenidone of claim 21, having a purity of at least 99.9% by weight or molar ratio.
23. A pharmaceutical composition comprising the pirfenidone of any one of claims 20-22 and a pharmaceutically acceptable excipient.
24. A method according to any one of the preceding claims, wherein the bromobenzene comprises less than about 0.1% by weight or molar ratio dibromobenzene.
25. A method of claim 24, wherein the bromobenzene comprises less than about 0.05% by weight or molar ratio dibromobenzene.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US61/183,588 | 2009-06-03 |
Publications (1)
Publication Number | Publication Date |
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OA16816A true OA16816A (en) | 2016-01-07 |
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