OA16730A - Thieno [2,3 -D] Pyrimidine derivatives and their use to treat Arrhythmia. - Google Patents

Abstract

The present invention relates to thienopyrimidine compounds which are potassium channel inhibitors. Pharmaceutical compositions comprising the compounds and their use in the treatment of arrhythmia are also described.

Description

The présent invention relates to thienopyrimidine compounds which are potassium channel inhibitors. Pharmaceutical compositions comprising the compounds and their use in the treatment of arrhythmia are also provided.

BACKGROUND ART

Ion channels are proteins that span the lipid bilayer of the cell membrane and provide an aqueous pathway through which spécifie ions such as Na⁺, K⁺, Ca²⁺ and CI' can pass (Herbert, 1998). Potassium channels represent the largest and most diverse sub-group of ion channels and they play a central rôle in regulating the membrane potential and controlling cellular excitability (Armstrong & Hîlle, 1998). Potassium channels hâve been categorized into gene families based on their amino acid sequence and their biophysical properties (for nomenclature see Gutman et al., 2003).

Compounds which modulate potassium channels hâve multiple therapeutic applications in several disease areas including cardiovascular, neuronal, auditory, rénal, metabolic and cell prolifération (Shieh et al., 2000; Ford et al., 2002). More specifically potassium channels such as Kv4.3, Kir2.1, hERG, KCNQ1/minK, and Kv1.5 are involved in the repolarisation phase of the action potential in cardiac myocytes. These potassium channels subtypes hâve been assocîated with cardiovascular diseases and disorders including long QT syndrome, hypertrophy, ventricular fibrillation, and atrial fibrillation, ail of which can cause cardiac failure and fatality (Marban, 2002).

The human delayed rectifier voltage gated potassium channel subunit, Kv1.5, is exclusively expressed in atrial myocytes and is beiieved to offer therapeutic opportunities for the management of atrial fibrillation for several different reasons (see review of Brendel and Peukert, 2002): (i) There is évidence that Kv1.5 underlies the cardiac ultrarapid delayed rectifier (Kv_(ur)) physiological current in humans due to similar biophysical and pharmacological properties (Wang et al., 1993; and Fedida et al., 1993). This has been supported with antisense oligonucleotides to Kv1.5 which hâve been shown to reduce Kv_(ur) amplitude in human atrial myocytes (Feng et al., 1997). (ii) electrophysiological recordings hâve demonstrated that Kv_(ur) is selectively expressed in atrial myocytes, and therefore avoids inducing potentially fatal ventricular arrhythmia through interfering with ventricular repolarisation (Amos et al., 1996; Li et al., 1996; and Nattel, 2002). (iii) Inhibiting Kv_(ur) in atrial fibrillation-type human atrial myocytes prolonged the action potential duration compared to normal healthy human atrial myocytes (Courtemanche et al., 1999). (iv) Prolonging the action potential duration by selectively inhibiting Kv1.5 could présent safer pharmacological interventions for protecting against atrial re-entrant arrhythmias such as atrial fibrillation and atrial flutter compared to traditional class III antiarrythmics, by prolonging the atrial refractory period while leaving ventricular refractoriness unaltered (Nattel et al., 1999, Knobloch et al., 2002; and Wirth et al., 2003). Class III antiarrythmics hâve been widely reported as a preferred method for treating cardiac arrhythmias (Colatsky et al., 1990).

Traditional and novel class III antiarrythmic potassium channel blockers hâve been reported to hâve a mechanism of action by directly modulating Kv1.5 or Kv(_Ufj. The known class III antiarrythmics ambasilide (Feng et al. , 1997), quinidine (Wang et al., 1995), clofilium (Malayev et al., 1995) and bertosamil (Godreau et al., 2002) hâve ail been reported as potassium channel blockers of Kv_(ur) in human atrial myocytes. The novel benzopyran dérivative, NIP-142, blocks Kv1.5 channels, prolongs the atrial refractory period and terminâtes atrial fibrillation and flutter in in vivo canine models (Matsuda et al., 2001), and S9947 inhibited Kv1.5 stably expressed in both Xenopus oocytes and Chinese hamster ovary (CHO) cells and Kv_(ur) in native rat and human cardiac myocytes (Bachmann et al., 2001). Elsewhere, other novel potassium channel modulators which target Kv1.5 or Kv_(ur) hâve been described for the treatment of cardiac arrhythmias, these include biphenyls (Peukert et al 2003), thiophene carboxylic acid amides (WO0248131), bisaryl dérivatives (WO0244137, WO0246162), carbonamide dérivatives (WO0100573, WO0125189) anthranîllic acid amides (W02002100825, W002088073, WO02087568), dihydropyrimidines (WO0140231), cycloakyl dérivatives (WO03063797), indane dérivatives (WO0146155 WO9804521), tetralin benzocycloheptane dérivatives (WO9937607), thiazolindone and metathiazanone dérivatives (WO9962891), benzamide dérivatives (W00025774), isoquinollne dérivatives (WO0224655), pyridazinones dérivatives (WO9818475 WO9818476), chroman dérivatives (WO9804542), benzopyran dérivatives (W00121610, W003000675, W00121609, WO0125224, W002064581), benzoxazine dérivatives (W00012492), and the novel compound A1998 purified from Océan material (Xu & Xu, 2000). General voltage gated potassium channel inhibitors hâve been reported which could also modulate Kv1.5 (US05753676, US05821251, EP0743936B).

Thienopyrimidines hâve been reported to be useful as antî-inflammatory, anti-fungal, antiosteoporosîs and anti-microbial agents, and as cardiovascular agents (acting through modulation of the phosphodiesterase group of enzymes or through modulation of the sodium/proton exchange system) amongst others.

Thieno[2,3-cf]-pyrimidines substituted in the 4-position with an optionally substituted benzylamine or phenethylamine moiety and in the 5-position with a methyl group may serve as anti-inflammatory or anti-osteoporosis agents (Katada et al., 1999). Such compounds were shown to modulate the activity of several cell types including leukocytes, which originale from hematopoietic precursor cells in the bone marrow. Increased activity in leukocytes can lead to various inflammatory diseases; therefore compounds cytotoxic to leukocytes could function as anti-inflammatory drugs. Such compounds are thought to suppress cellular activity by binding to integrins on the surface of leukocytes and preventing downstream cellular signalling events. Thieno[2,3-d]pyrimidines substituted in the 4-position with heteroarylthiols, aryl thioîs, arylmethyl thiols, heteroarylamines, benzylamine, hydroxyl and chloro groups may also be useful anti-inflammatory agents (Stewart et al., 2001). This sériés of compounds were shown to inhibit induced expression of cell adhesion molécules on the luminal surface of vascular endothélial thus preventing the adhesion of leukocytes at the site of inflammation.

Thieno[2,3-d]pyrimidines with a substituted hydrazine in the 4-position and a phenyl group in the 5 position (Hozien et al., 1996), tetrahydrobenzo[b]thieno[2,3-d]pyrimidÎnes (Ismail et al., 1995), thieno[2,3-c(|pyrimidines which hâve a hydrogen, chloro, hydrazine, heterocyclyl, amino, methyl, ethyl or phenyl group in the 2-position, an alkylamino, alkylarylamtno, amino, dialkylamino or hydrazino substituent in the 4-position, a hydrogen or methyl group in the 5-position, a hydrogen, methyl acetamide or phenyl group in the 6-position or a tetramethylene in the 5,6-position (GB7549025), and the lead sériés of 5-phenyl- and 5,6-tetramethylenethieno[2,3-c(]pyrimidines with methyl or phenyl in the 2-position and alkylamino or arylamino in the 4- position (Konno et al., 1989) hâve ail been shown to hâve anti-microbial activity. Tetrahydrobenzothieno[2,3-c(]pyrimidine with the 2-oxo-3-pyrrolidinylmethylene-hydrazino moiety in the 4-position showed some herbicidal activity against velvet leaf (Ram et al., 1981). It has also been reported that 4chlorotetrahydrobenzothieno[2,3-d]pyrimidine is herbicidal, tetrahydrobenzothieno-[2,3djpyrimidines with a thiol, hydrazine, 2-fluoroanilino, 3-fluoroanilino or 4-diethylanilino substituent in the 4-position are bactericidal against Streptococcus fecales and tetrahyrobenzothieno[2,3djpyrimidines with a 2,4-dichlorobenzylamino or 2-fluoroanilino substituent in the 4-position are fungicidal against Pythium (Ram, 1979). Thieno[2,3-c(]pyrimidines with a hydrogen, hydroxyl, thiol, halogen or cyano group in the 2-position, alkylamino, arylalkylamino or hydroxyalkyl amino groups in the 4-position, a hydrogen, alkyl or halogen in the 5- and/or 6- position or alkylene in the 5,6position hâve been reported as tick-control agents (AU 521790).

Elsewhere, tetrahydrobenzo[b]thieno[2,3-c/]pyrimidines exhibited anti-tumour activity (Shehata et al., 1996) and analgésie activity half that of aspirin (Moneer et ah, 1994), a sériés of thieno[2,3djpyrimidines with 4-alkylamino or arylamino, 5-H or 5-methyl, 6-methyl or 5,6-tetramethylene were shown to hâve potential as anticytokinins (Jordis et ah, 1986), a sériés of 5,6-dimethyl-thieno[2,35 c/]pyrimidines and 5,6-tetramethylenethieno[2,3-d]pyrimidines, both substituted in the 2-position with arylamines or heterocyclic amines and in the 4-position with arylamines displayed blood platelet aggregation inhibiting properties (DD 226893), pyrano- and thiopyrano[3,4-b]thîeno[5,4d]pyrimidines with the 4-position substituted with amino, butylamine, aniline, cyclohexylamine, benzylamine, phenethylamine and 2-hydroxyethylamine hâve been reported to exhibit 10 anticonvulsive activity (Noravyan et ah, 1977), and 4-[(Benzo-2,1,3-thiadiazolyl-4)amino]-5,6,7,8tetrahydrobenzothieno-(2,3-d)-pyrimidine has been reported to possess anthelmintic activity in larval alveolar echinococcosis (RU 2116309).

Thieno[2,3-cf]pyrimidines with a substituted amino group at the 4-position, hydrogen, alkyl or halo 15 substitution at the 5 and 6-positions and an alkyl chain at the 2-position are claimed to be inhibitors of phosphodiesterase V and useful in the treatment of cardiovascular diseases and for disturbances in potency (DE10104802).

Elsewhere, 5-alkyl thieno[2,3-d]pyrimidines with a piperazinyl substituent at the 4-position were 20 found to be inhibitors of the sodium/proton exchanger and useful in the treatment of various cardiovascular disorders, including angina pectoris and arrhythmia (WO 01/27107).

4-[(phenyl)amino]-thieno[2,3-d]pyrimidines bearing a 5-thiophenyl substituent and a 2-methyl substituent were found to hâve molluscicidal activity (Hosni et al, Acta Poloniae Pharmaceutica, 25 1999,56(1),49-56).

Recently thienopyrimidines hâve also been reported as potent VEGFR inhibitors (Munchhof, 2004).

Several publications disclose compounds which are indicated as acting on potassium channels. 30 Thus, US6531495 discloses 2’-aminomethylbiphenyl-2-carboxamides, W02002/100825 discloses anthranillic acid amides as antiarrhythmics and W02002/036556 discloses acylaminoalkylbenzenesulfonamides as cardiovascular agents.

Thienopyrimidine compounds that are useful as potassium channel inhibitors, particularly for inhibiting potassium channels Kv1.5 or Kv_(ur), are reported in WO 2004/111057.

DISCLOSURE OF THE INVENTION

A first aspect of the invention provides a compound of formula (la)

or a pharmaceutically acceptable ester or sait thereof.

In one embodiment, the compound is of formula (Ib)

In another embodiment, the compound is of formula (le)

(le).

In another embodiment, the compound of formula (la) comprises a mixture of the compounds of formulae (lb) and (le). In a further embodiment, the compound of formula (la) comprises a racemic mixture of the compounds of formulae (lb) and (le). In an alternative further embodiment, the compound of formula (la) comprises an enantiomeric excess of the compound of formula (lb) or an enantiomeric excess of the compound of formula (le).

A second aspect of the invention provides a pharmaceutical composition comprising at least one of the above compounds and, optionally, one or more pharmaceutically acceptable excipients.

The compounds and compositions of the invention are potassium channel inhibitors that are particularly useful for inhibiting potassium channels Kv1.5 or Kv_(ur) for the treatment of cardiac arrhythmia in the atria such as atrial fibrillation. This invention is not lîmited to treating cardiac arrhythmias, the compounds also being useful to treat diseases which require potassium channel inhibition (e.g. Shieh et al., 2000; Ford et al., 2002).

A third aspect of the invention therefore provides a method of potassium channel inhibition, comprising administering to a subject an effective amount of at least one compound or composition of the invention. This aspect of the invention further provides a compound or composition of the invention for use in potassium channel inhibition. In addition, this aspect of the invention further provides the use of a compound of the invention for the manufacture of a médicament for use in potassium channel inhibition. As used herein, a “method of potassium channel inhibition and “use in potassium channel inhibition include methods and uses for treating or preventing a disorder which responds to the inhibition of potassium channel function. The disorder may be arrhythmia.

The compounds of the invention hâve advantageous properties over those of the prior art, in particular in terms of potency and/or selectivity.

DETAILED DESCRIPTION OF THE INVENTION

Racemic mixture

A “racemic mixture contains approximately equal amounts of the compounds of formula (lb) and formula (le). In other words, a compound or composition comprising a racemic mixture” of the compounds of formula (Ib) and formula (le) contains an approximately 1:1, or 50:50, mixture of the compounds.

Enantiomeric excess

A compound or composition comprising an “enantiomeric excess” of the compound of formula (Ib) or of the compound of formula (le) comprises more of that enantiomer than the other (also known as a scalemic mixture).

The enantiomeric excess is the excess of one compound over the other, expressed as a percentage of the whole. For instance, a 98:2 mixture of the compound of formula (Ib) to the compound of formula (le) has a 96 % enantiomeric excess of the compound of formula (Ib). Thus, the compounds and compositions of the invention may comprise an enantiomeric excess of the compound of formula (Ib) of at least 5 %, 10 %, 15 %, 20 %, 25 %, 30 %, 35 %, 40 %, 45 %, 50 %, 55 %, 60 %, 65 %, 70 %, 75 %, 80 %, 85 %, 90 %, 95 % or up to 100 % (/.e. enantiomerically pure, up to the détection limit of purity). Alternatively, the compounds and compositions of the invention may comprise an enantiomeric excess of the compound of formula (le) of at least 5 %, 10 %, 15 %, 20 %, 25 %, 30 %, 35 %, 40 %, 45 %, 50 %, 55 %, 60 %, 65 %, 70 %, 75 %, 80 %, 85 %, 90 %, 95 %orupto 100%.

R and S nomenclature

As used herein, the term “R or “S isomer refers to the two possible enantiomers according to the Cahn-Ingold-Prelog system adopted by the International Union of Pure and Applied Chemistry (IUPAC). Thus, the compound of formula (Ib) is the “S-isomer” and the compound of formula (le) is the “R-isomer.

Pharmaceutically acceptable ester or sait thereof

The term “pharmaceutically acceptable ester includes compounds of the invention in which the hydrogen atom of the alcohol group may be replaced to form an ester (e.g. the hydrogen atom may be replaced by -C(O)C_1Æalkyl).

The term “pharmaceutically acceptable sait includes a sait prepared from pharmaceutically acceptable non-toxic acids or bases including inorganic or organic acids and bases.

Pharmaceutically acceptable acid addition salts of the compounds of the invention include, but are not limited to, those of inorganic acids such as hydrohalic acids (e.g. hydrochloric, hydrobromic and hydroiodic acid), sulfuric acid, nitric acid, and phosphoric acids. In addition, pharmaceutically acceptable acid addition salts of the compounds of the invention include, but are not limited to, those of organic acids such as aliphatic, aromatic, carboxylic and sulfonic classes of organic acids, examples of which include: aliphatic monocarboxylic acids such as formic acid, acetic acid, propionic acid or butyric acid; aliphatic hydroxy acids such as lactic acid, citric acid, tartaric acid or malic acid; dîcarboxylic acids such as maleic acid or succinic acid; aromatic carboxylic acids such as benzoic acid, p-chlorobenzoic acid, phenylacetic acid, diphenylacetic acid or triphenylacetic acid; aromatic hydroxyl acids such as o-hydroxybenzoic acid, p-hydroxybenzoic acid, 1hydroxynaphthalene-2-carboxylic acid or 3-hydroxynaphthalene-2-carboxylic acid; and sulfonic acids such as methanesulfonic acid, ethanesulfonic acid or benzenesulfonic acid. Other pharmaceutically acceptable acid addition salts of the compounds of the invention include, but are not limited to, those of glycolic acid, glucuronic acid, furoic acid, glutamic acid, anthranilic acid, salicylic acid, mandelic acid, embonic (pamoîc) acid, pantothenic acid, stearic acid, sulfanilic acid, algenic acid, and galacturonic acid.

Pharmaceutically acceptable basic salts of the compounds of the invention include, but are not limited to, métal salts such as alkali métal or alkaline earth métal salts (e.g. sodium, potassium, magnésium or calcium salts) and zinc or aluminium salts. In addition, pharmaceutically acceptable basic salts of the compounds of the invention include, but are not limited to, salts formed with ammonia or pharmaceutically acceptable organic amines or heterocyclic bases such as ethanolamines (e.g. diethanolamine), benzylamines, N-methyl-glucamine, amino acids (e.g. lysine) or pyridine.

Synthesis

Compounds of formula (I) may be prepared as the racemate, as a scalemic mixture, or as a chirally pure enantiomer using routes described in the scheme 1 below:

<^v) (IV) (m)

This includes the préparation of compounds of formula (I) using the “linear route” analogous to the synthetic route disclosed in WO20Û4/111057 from compounds of formula (II) and aminoethanol. Typieally, this reaction is carried out using a coupling reagent such as 1-ethyl-3-(310 dimethylaminopropyl)-carbodiimide (EDC) or 2-(7-aza-1H-benztriazole-1-yl)-1,1,3,3tetramethyluronium hexafluorophosphate (HATU) utilising standard methods familiar to those skilled in the art such as reaction in solvent such as tetrahydrofuran, acetonitrile or dimethylformamide at a range of températures from ambient to reflux température. Alternatively, compounds of formula (I) may be prepared from compounds of formula (III) by displacement of the 15 2-chloro substituent with a compound of formula (IX) in the presence of a base such as N,N16730

ΙΟ diisopropylethylamine and a solvent such as N-methyl pyrrolidinone with conventional heating or

Compounds of formula (II) may be prepared from a compound of formula (III) by displacement of the 2-chloro substituent with commercially available nîpecotic acid, in the presence of a base such as Ν,Ν-diisopropylethylamine and a solvent such as N-methyl pyrrolidinone with conventional heating or microwave irradiation.

(III)

Compounds of formula (III) are readily synthesised from compounds of formula (IV) by a nucleophilic substitution reaction with 2-aminomethylpyridine, optionally in the presence of a solvent and a base, and optionally at elevated température or with microwave irradiation. Preferably the solvent (if présent) is an alcohol, preferably éthanol and the base is a hindered nitrogen base such as triethylamine. The reaction is carried out at ambient températures.

A compound of formula (IV) may be synthesised by reaction of a compound of formula (V) with a chlorinating reagent such as phenylphosphonic dichloride or phosphorous oxychloride.

(V)

Compounds of formula (V) may be synthesised by the reaction of a compound of formula (VI) with an alkali métal cyanate, preferably potassium cyanate.

(VI)

A compound of formula (VI) can be prepared by the “Gewald réaction in which a compound of formula (VII) is reacted under basic conditions and in a suitable solvent such as éthanol, with powdered sulphur. Preferably the base is diisopropylethylamine (Hünig's base) and the solvent may be an alcohol, preferably éthanol, and the reaction is carried out between 25 and 65°C.

Compounds of formula (VII) can be prepared by the Knoevenagel condensation reaction by heating compound of formula (VIII) with ethylcyanoacetate (NCCH^CC^Et) in the presence of an acid and ammonium acetate in a suitable solvent such as toluene, optionally with azeotropic water removal. Preferably the acid is acetic acid. This gives the alkylidene cyano ester as a pair of (E and Z) géométrie isomers.

HN

(IX)

Compound of formula (IX) may be prepared from compound of formula (X) by hydrolysis of the tbutyl carbamate (BOC) protecting group with a strong acid in a solvent such as dichlorométhane. Typically the acid is trifluoroacetic acid.

Compound of formula (X) may be prepared from a compound of formula (XI) and aminoethanol. Typically, this reaction is carried out using a coupling reagent such as 1 -ethyl-3-(3dimethylaminopropyl)-carbodiimide (EDC) or 2-(7-aza-1H-benztriazole-1-yl)-1,1,3,3tetramethyluronium hexafluorophosphate (HATU) utilising standard methods familier to those skilled in the art such as reaction in solvent such as tetrahydrofuran, acetonitrile or dimethylformamide at a range of températures from ambient to reflux température.

Pharmaceutical compositions

As discussed herein, the compounds of the invention are useful in the treatment of various conditions. Thus, the second aspect of the invention provides a pharmaceutical composition or formulation comprising at least one compound of the invention and optionally one or more pharmaceutically acceptable excipients.

Typical pharmaceutically acceptable excipients include:

• diluents, e.g. lactose, dextrose, sucrose, mannitol, sorbitol, cellulose and/or glycine;

• lubricants, e.g. silica, talcum, stearic acid, its magnésium or calcium sait and/or polyethyleneglycol;

• binders, e.g. magnésium aluminum silicate, starch paste, gelatin, tragacanth, methylcellulose, sodium carboxymethylcellulose and/or polyvinylpyrrolidone;

• disintegrants, e.g. starches, agar, alginic acid or its sodium sait, or effervescent mixtures; and/or • absorbants, colorants, and/or sweeteners.

The compositions of the invention may be presented in unit dose forms containing a predetermined amount of each active ingrédient per dose. Such a unit may be adapted to provide 5-100mg/day of the compound, preferably either 5-15mg/day, 10-30mg/day, 25-50mg/day 40-80mg/day or 60100mg/day. For compounds of the invention, doses in the range 100-1000mg/day are provided, preferably either 100-400mg/day, 300-600mg/day or 500-1 OOOmg/day. Such doses can be provided in a single dose or as a number of discrète doses. The ultimate dose will dépend on the condition being treated, the route of administration and the âge, weight and condition of the patient and will be at the doctor's discrétion.

The compositions of the invention may be adapted for administration by any appropriate route, for example by the oral (including buccal or sublingual), rectal, nasal, topical (including buccal, sublingual or transdermal), vaginal or parentéral (including subcutaneous, ïntramuscular, intravenous or intradermal) route. Such formulations may be prepared by any method known in the art of pharmacy, for example by bringing into association the active ingrédient with the carrier(s) or excipient(s).

Pharmaceutical formulations adapted for oral administration may be presented as discrète units such as capsules or tablets; powders or granules; solutions or suspensions in aqueous or nonaqueous liquids; edible foams or whips; or oil-in-water liquid émulsions or water-in-oil liquid émulsions.

Pharmaceutical formulations adapted for transdermal administration may be presented as discrète patches intended to remain in intimate contact with the epidermis of the récipient for a prolonged period of time. For example, the active ingrédient may be delivered from the patch by iontophoresis as generally described in Pharmaceutical Research, 3(6), 318 (1986).

Pharmaceutical formulations adapted for topical administration may be formulated as ointments, creams, suspensions, lotions, powders, solutions, pastes, gels, sprays, aérosols or oils.

For applications to the eye or other external tissues, for example the mouth and skin, the formulations are preferably applied as a topical ointment or cream, When formulated in an ointment, the active ingrédient may be employed with either a paraffinic or a water-miscible ointment base. Alternatively, the active ingrédient may be formulated in a cream with an oil-in-water cream base or a water-in-oil base.

Pharmaceutical formulations adapted for topical administration to the eye include eye drops wherein the active ingrédient is dissolved or suspended in a suitable carrier, especially an aqueous solvent.

Pharmaceutical formulations adapted for topical administration in the mouth include lozenges, pastilles and mouth washes.

Pharmaceutical formulations adapted for rectal administration may be presented as suppositories orenemas.

Pharmaceutical formulations adapted for nasal administration wherein the carrier is a solid include a coarse powder having a particle size for example in the range 20 to 500 microns which is administered in the manner in which snuff is taken, i.e. by rapid inhalation through the nasal passage from a container of the powder held close up to the nose. Suitable formulations wherein the carrier is a liquid, for administration as a nasal spray or as nasal drops, include aqueous or oil solutions of the active ingrédient.

I6

Pharmaceutical formulations adapted for administration by inhalation include fine particle dusts or mists which may be generated by means of various types of metered dose pressurised aérosols, nebulizers or insufflators,

Pharmaceutical formulations adapted for vaginal administration may be presented as pessaries, tampons, creams, gels, pastes, foams or spray formulations.

Pharmaceutical formulations adapted for parentéral administration include aqueous and nonaqueous stérile injection solutions which may contain anti-oxidants, buffers, bacteriostats and solutés which render the formulation isotonie with the blood of the intended récipient; and aqueous and non-aqueous stérile suspensions which may include suspending agents and thickening agents. The formulations may be presented in unit-dose or multi-dose containers, for example sealed ampoules and vials, and may be stored in a freeze-dried (lyophilized) condition requirîng only the addition of the stérile liquid carrier, for example water for injections, immediately prior to use. Extemporaneous injection solutions and suspensions may be prepared from stérile powders, granules and tablets.

Preferred unit dosage formulations are those containing a daily dose or sub-dose, as herein above recited, or an appropriate fraction thereof, of an active ingrédient.

It should be understood that in addition to the ingrédients particulariy mentioned above, the formulations may also include other agents conventional in the art having regard to the type of formulation in question, for example those suitable for oral administration may include flavouring agents.

MODES FOR CARRYING OUT THE INVENTION

The following protocols describe préparation of:-

1. racemate made using “convergent route (Examples 1 to 6)

2. enantiomers made using “convergent route (Examples 1 to 5 and 7 to 12)

3. enantiomers made using linear route (Examples 1 to 5 and 13 to 16)

Synthesis and détermination of enantiomers

The desired enantiomerically pure compound was obtained by the careful sélection of reagents and the use of appropriate experimental conditions and sequence in particular with regard to steps forming the chiral center and subséquent reaction. It was determined during the course of synthesis that the “linear route was less prone to racémisation as the final amide forming bond could be carried out at lower température as opposed to the convergent route which provided better yields but with détectable racémisation.

For the “linear route, pure enantiomers of the nipecotic acid were obtained by classical resolution of cheap commercially available racemic nipecotic acid using 1-(S)-camphor sulfonic acid as the resolving agent, determining ee analysis after forming a BOC dérivative of a sample.

The enantiomeric purity was determined by Chiral HPLC.

Analytical methods

Proton magnetic résonance (¹H NMR) spectra were recorded on a Varian 400MHz Mercury Plus spectrometer. Ail spectra were determined in dmso-d6 unless otherwise stated and chemical shifts are reported in (sigma) units downfield from the internai standard tetramethylsilane (TMS) and interproton coupling constants are reported in Hertz (Hz), splitting paterns are designated as follows: s, singlet; d, doublet; t, triplet; q, quartet; m, multiplet; br, broad peak; dd, doublet of doublet; dt, doublet of triplet; bs, broad singlet; dq, doublet of quartet.

IR spectra were determined on a Perkin Elmer Spectrum One instrument.

Mass spectra were determined on an Agilent 6310 Ion trap instrument.

HPLC analysis (method (a)) was carried out on a Waters 2695 system using ZORBAX SB C-18 (4.6 x 50mm) column;

Mobile phase: A: 0.05%TFA (AQ,) B: 0.05% TFA (MeCN); T%B: 0/20, 5/90, 8/90, 8.1/20; flow rate 1.0mL/min;

Chiral column: Chiralpak IC (4.6X250mm)5u, mobile phase: A: Hexane, B: EtOH (70:30); flow rate 0.8ml/min run over 40 minutes.

Melting points were determined on an EX-Melt instrument (Model: MPA120).

Alternative^, HPLC analysis (method (b)) was carried out with: Waters 616 fluid handling system, Waters 996 photodiode array detector.

Chiral column: Daicel Chiralpak AD-H (Chiral technologies) reporting chiral purity at 244nm; mobile phase 80%Hexanes:20%EtOH; fiow rate 0.8ml/min; temp 40 °C.

Mass spectra were determined on an Agilent 1100 sériés instrument (Model: G1946C).

Using the information outlined herein the following compounds can be synthesised which are given by way of example only. The pharmacological profile of compounds of the présent invention can readily be assessed by those skilled in the art using routine expérimentation, such as procedures ]0 and techniques illustrated herein and described in detail in Ford et al., 2002.

Example 1 (Z)-2-Cyano-3-phenyl-but-2-enoic acid ethyl ester (VII)

A stirred mixture of acetophenone (VIII) (180g, 1.5mol), ethyl cyanoacetate (170g, 1.3mol), ammonium acetate (23.1g), acetic acid (72g) and toluene (300ml) was heated under reflux for 18 20 hours while water was removed from the reaction by azeotropic distillation. The mixture was allowed to cool to ambient température, toluene (100ml) was added, then the mixture was washed with water (3 x 100ml). The combined aqueous washings were shaken with toluene (50ml), then the combined toluene solutions were dried over magnésium sulphate, filtered and the solvent was removed in vacuo. The residual oil was distilled under reduced pressure to give 2-cyano-3-phenyl25 but-2-enoic acid ethyl ester as an oil (309g) which was used without further purification.

Example 2

2-Amino-4-phenyl-thiophene-3-carboxylic acid ethyl ester (VI)

2-Cyano-3-phenyl-but-2-enoic acid ethyl ester (513.25g, 2.3mol) was added at ambient température to a vigorously-stirred suspension of powdered sulfur (76g, 2.3mol) in éthanol (500ml). Diethylamine (200ml) was added in portions over 20 minutes, during which time the température of the reaction rose to 62°C. The mixture was allowed to cool to 36°C, then it was heated to 50°C and stirring at that température was continued for 1 hr. After this time, stirring was discontinued, the hot solution was removed by décantation from unreacted sulfur, then it was allowed to cool to ambient température. The resulting solid was collected by filtration, washed with a little cold éthanol and dried in vacuo to give 2-amino-4-phenylthiophene-3-carboxylic acid ethyl ester as an orange solid (195g) which was used without further purification.

Example 3

5-Phenyl-1 H-thieno[2,3-d]pyrimidine-2,4-dione (V)

2-Amino-4-phenyl-thiophene-3-carboxylic acid ethyl ester (2.0g, 8.1 mmol), and Potassium Cyanate (Aldrich, 2.0g, 24.3mmol) were added to glacial acetic acid (VWR, 20ml) and stirred at ambient température for 18h, The reaction was diluted with water (50ml) and the résultant precipitate filtered, washed with water and dried to a damp cake. The solid was suspended in water (100ml) and made alkaline to pH 12-14 by the addition of concentrated sodium hydroxide. The résultant suspension was heated at 100°C for 2h with stirring, then cooled to ambient température and acidified by the addition of glacial acetic acid. The resulting solid was collected by filtration, washed with water and dried in vacuo at 40°C to give 5-Phenyl-1H-thieno[2,3-d]pyrimidine-2,4-dione as a white solid. Yield = (1.1g, 56%).

Example 4

2,4-Dichloro-5-phenyl-thieno[2,3-d]pyrimidine (IV)

N Cl

A stirred mixture of 5-Phenyl-1H-thieno[2,3-d]pyrimidine-2,4-dione (1.07g, 4.39mmol) and phenyl phosphonic dichloride (Aldrich, 10ml, excess) was heated at 150°C for 7h then allowed to stand at ambient température for 18hrs. The resulting dark solution was poured into ice-water and extracted with DCM (3 x 150ml). The combined extracts were washed with saturated sodium hydrogen carbonate solution (150ml) and dried (MgSO₄). The solvent was removed tn vacuo and the oily residue triturated with 40-60°C petroleum ether to give 2,4-Dichloro-5-phenyl-thieno[2,3djpyrimidine as a pale yellow solid. Yield = (0.82g, 66%).

Example 5 (2-Chloro-5-phenyl-thieno[2,3-d]pyrimidin-4-yl)-pyridin-2-ylmethyl-amine (III)

A mixture of 2,4-Dichloro-5-phenyl-thieno[2,3-d]pyrimidine (1.77g, 6.3mmol), 2aminomethylpyridine (Aldrich, 782OI, 7.6mmol), and triethylamine (VWR, 1.06ml, 7.63 mmol ) were refluxed in éthanol (30ml) for 3 hrs. On cooling, the reaction was poured into water (300ml) and stirred for 1 hr. The resulting precipitate was filtered, washed with water (2 x 30ml) and dried under vacuum at 40°C to give (2-Chloro-5-phenyl-thieno[2,3-d]pyrimidin-4-yl)-pyridin-2-ylmethyl-amine as a pale-yellow solid. Yield = (1.55g, 70%).

Racemate - convergent route

Example 6 (Racemic) 1-{5-Phenyl-4-[(pyridin-2-ylmethyl)-amino]-thieno[2,3-d]pyrimidin-2-yl}-piperidine3carboxylic acid (2-hydroxy-ethyl)-amide (la)

(2-Chloro-5-phenyl-thieno[2,3-d]pyrimidin-4-yl)-pyndin-2-ylmethyl-amine (44mg, 0.124mmol),

Piperidine-3-carboxylïc acid(2-hydroxyethyl)amide (Fluorochem, 32mg, 0.188mmol, 1.5eq) and Ν,Ν-diisopropylethylamine (Aldrich, 0.188mmol) were dissolved in N-Methyl Pyrrolidinone (1.5ml) in a Biotage microwave tube and heated to 200°C and maintained at this température for 30 min. On cooling, the solvents were removed in vacuo. The residue was triturated with DCM (2x10ml) and the extracts combined, concentrated and purified by prep TLC (eluent 10% MeOH/DCM) to give the product as a yellow oil, which slowly solidified on standing to a waxy solid. The waxy solid may be 5 converted to a free-flowing powder by stirring in diethyl ether for 1 -2h (0.5g in 10ml). Yield=18.3mg (30%)

Enantiomers - convergent route

Example 7 (S)-3-(2-Hydroxy-ethylcarbamoyl)-piperidine-1 -carboxylic acid tert-butyl ester (S)-Piperidine-1,3-dicarboxylic acid 1-tert-butyl ester (500mg, 2.2mmol), HATU (833 mg, 2.2mmol) and Di-isopropylethylamine (761 □!_, 4.4mmol) were stirred in dry DCM (10ml) in an ice bath for 5 min, then at room température for 5 min. Ethanolamine (198 DL, 3.28mmol) was added and the reaction stirred at room température for 3 hrs. The reaction was diluted with DCM (40ml), washed with water (50ml), the DCM layer separated and dried (MgSO₄) and concentrated. The residue was columned on silica (20g isolute). Eluting: MeOH/DCM 0-5% 5CV, MeOH/DCM 5%-5% 10CV,

MeOH/DCM 5-10% 5CV. TLC visualized with KMnO₄. This yielded the product as a clear oil (327mg).

Sîmilarly was prepared:

Example 8 (R)-3-(2-Hydroxy-ethylcarbamoyl)-piperidine-1-carboxylic acid tert-butyl ester

Example 9 (S)-Piperidine-3-carboxylic acid (2-hydroxy-ethyl)-amide

The product of the above reaction was stirred in 1:1 TFA/DCM for 2hrs then concentrated in vacuo to an oil. This was dissolved in MeOH (5ml) and loaded onto a 5g SCX cartridge. The cartridge was washed with MeOH (10ml), then the product eluted with 2M NH₃/MeOH (10ml). The fraction was concentrated to give a white solid. Yield = 260mg.

Similarly was prepared:

Example 10 (R)-Piperidine-3-carboxylic acid (2-hydroxy-ethyl)-amide

Example 11 (S)-1-{5-Phenyl-4-ï(pyridjn-2-ylmethyl)-amino]-thieno[2,3-d]pyrimidin-2-yl}-piperidine-3carboxylic acid (2-hydroxy-ethyl)-amide (lb)

(S)-Piperidine-3-carboxylic acid (2-hydroxy-ethyl)-amide was reacted with (2-Chloro-5-phenylthieno[2,3-d]pyrimidin-4-yl)-pyridin-2-ylmethyl“amine as in Example 6 above to give (S)-1-{5Phenyl-4-[(pyridin“2-ylmethyl)-amino]-thieno[2,3-d]pyrimidin-2-yl}'piperidine-3-carboxylic acid (2hydroxy-ethyi)-amide as a yellow foam (188mg).

Similarly was prepared:

Example 12 (R) -1-{5-Phenyl-4-[(pyridin-2-yimethyl)-amino]-thieno[2,3-d]pyrimidin-2-yl}-piperidine-3carboxylic acid (2-hydroxy-ethyl)-amide (le)

Enantiomers - linear route via

Example 13 (S) -Nipecotic acid-(S)-Camphorsulfonate sait

Chiral resolution of (S) Nipecotic acid from commercial racemic mixture

To a solution of (S)-camphorsulfonic acid (18kg, 77mol) in acetone (127kg) at 55-58 °C, a solution of (R,S)-nipecotic acid (10kg, 77mol) in water (20kg) was quickly charged, The mixture was maintained at 55-58 °C until ail solids were dissolved. The solution was slowly cooled to 20-25 °C to precipitate the sait, then stirred overnight, and isolated, To further increase the diastereomeric purity, the resulting sait was re-crystallized from acetone (16kg) and water (4kg) at 55-58 °C. Again, the hot solution was cooled to 20-25 °C, stirred overnight, and isolated to give the purified (S)-nipecotic acid-(S)-camphorsulfonate sait (14kg).

Example 14 (S)-Piperidine-3-carboxylic acid hydrochloride

(S)-Piperidine-1,3“dicarboxylic acid 1-tert-butyl ester (20kg, 87.2mol) was slurried in acetic acid (189kg) and cooled to 15 °C. An excess of hydrogen chloride gas (9.6kg) was charged and stirred for -4 hours to complété deprotection. The slurry was isolated and filter-cake rinsed with acetic acid (2 x 31.5kg). The filter cake was then vacuum dried to obtain product (14.4kg).

Example 15 (S)-1-{5-Phenyl“4-[(pyridin-2-ylmethyl)-amîno]-thieno[2,3-d]pyrimidin-2-yl}-piperidine-3carboxylic acid

OH (2-Chloro-5-phenyl-thieno[2,3-d]pyrimidin-4-yl)-pyridin-2-ylmethyl-amine (5.9kg, 16.7mol) and (S)nipecotic acid hydrochloride (4.15kg, 25.1 mol) were dissolved in butyrolnitrile (13.9kg). An excess of diisopropylethylamine (8.6kg, 66,9mol) was added and the mixture heated to 110 °C for 24 to 48 hours to complété reaction. With coupling complété (<2% nipecotic acid remaining), the reaction was cooled to room température and water (29kg) was charged. The mixture pH was adjusted to ~10 with 25% aqueous sodium hydroxide (4.5L) and the layers separated. The product aqueous layer was extracted twice with ethyl acetate (15.9L) then methylene chloride (23.5kg) was added to the aqueous layer and the pH adjusted to -2.5 with concentrated hydrochloric acid (6.3kg). The layers were separated and the aqueous layer re-extracted with methylene chloride (2 x 15.7kg). The methylene chloride layers were combined and washed with water (18kg) then dried over sodium sulfate (5.9kg) and product solution was held for processing in next step (Example 16).

Example 16 (S)-1-{5-Phenyl-4-[(pyridin-2-ylmethyl)-amino]-thieno[2,3-d]pyrimidin-2-yl}-piperidine-3carboxylic acid (2-hydroxy-ethyl)-amide (Ib)

OH (SJ-l-ÎS-Phenyl-A-Kpyndin^-ylmethylJ-aminoJ-thieno^.S-dlpyrimidin-Z-ylJ-piperidine-S-carboxylic acid solution (7.4kg, 16.63mol) (from Example 15) was cooled to 0 °C and diisopropylamine (4.51kg, 35mol) and ethanolamine (2.03kg, 33.3mol) were added. Maintaining the reaction température below 10 ’C, bezotriazolyl tetramethyluronium-BF4 (TBTU) (5.9kg, 18.3mol) was charged in portions then stirred at -5 C until the coupling was complété. The reaction solution was then filtered to remove TBTU salts and washed with water (22.2 L), followed by two washes with citric acid/sodium hydroxide buffer aqueous solution (pH ~5) (2.88kg, 15mol), and finally with a brine solution (4L). Subsequently, the mixture was charged with butyronitrîle (17.4L) and partially stripped to precipitate the diastereomeric product. The slurry was filtered to remove diastereoisomer and the filtrâtes stripped further to -1/2 volume. To the mix heptanes (30.4L) was charged to precipitate product and the slurry cooled to room température. The slurry was filtered, rinsed with heptanes (10.1L), and vacuum dried to obtain product (4.1kg).

(R)-1-{5-Phenyl-4-[(pyridin-2-ylmethyl)-amino]-thieno[2,3-d]pyrimidin“2-yl}-piperidine-3-carboxylic acid (2-hydroxy-ethyl)-amide (le) may be prepared according to a route analogous to Examples 13 to 16.

Example 17

Analytical data for the compounds represented by the above examples are shown in the table below.

Ex	NMR spectrum 1H (400MHz; dmso-d6)	HPLC (RT) mins	Mass Spec (M+1)	Chiral HPLC (method (a))	MP (DC)	FT-IR □ max (cm*1)
2	0.91 (3H, t), 3.96 (2H,q), 6.15 (1H, s)7.3 (5H, m)	4.8	248 (99.5%)
3	6.67 (1H, s), 7.3 (3H, m), 7.47 (2H, m)	(uplc)	245 (98.9%)
4	7.51 (5H, m), 7.99 (1H,s)	(uplc)	282 (92%
5	4.64 (2H, s), 7.07 (1H, m), 7.23 (1H, m), 7.4 (1H, d), 7.55 (6H, m), 7.75 (1 H, dt), 8.21 (1H, m)	(uplc 1.75)	353 (97.6%)
6	1.3 (1H. m), 1.6 (2H, m), 1.8 (1H, m), 2.3 (1H, m), 2.8 (2H, m), 3.1 (2H, m), 3.4 (2H, m), 4.6 (5H, m), 6.3 (1H,m), 6.95 (1H,s), 7.2-7.3 (2H, m), 7.5 (5H, m), 7.7 (1 H. m). 7.9 (1 H, m), 8.3 (1H, m)	2.67 (uplc 0.91)	489 (99.8%)		190- 194	3338, 3298, 3098, 3009, 2931, 2847, 1642, 1556, 1517, 1504, 1484, 1438, 1386, 1321, 1300, 1255, 1220, 1203, 1139, 1062
11 (S)	1.3 (1H, m), 1.6 (2H, m), 1.8 (1H, m), 2.3 (1H, m), 2.8 (2H, m), 3.2 (2H, m), 3.4 (2H, m), 4.6 (5H, m), 6.3 (1H, t), 6.95 (1H,s),	-	489.3	98.3% RT=18.4 min	61- 65	3426, 3357, 1649

	7.2 (1H, dd), 7.3 (1H, d), 7.5 (5H, m). 7.7 (1H, dd), 7.9 (1H, m), 8.3 (1H, m)
12 (R)	1.3 (1H. m), 1.6 (2H, m), 1.8 (1H, m), 2.3 (1H, m), 2.8 (2H, m), 3.2 (2H, m), 3.4 (2H, m), 4.6 (5H, m), 6.3 (1H,t), 6.95 (1H,s), 7.2 (1H, dd), 7.3 (1H, d), 7.5 (5H, m), 7.7 (1H, dd), 7.9 (1H, m), 8.3 (1H, m)		489.3	97.6% RT= 14.87 MIN	71- 76	3425, 3352, 1649

Example 18

Kv1.5 Electrophysiology Method

The ability of the compounds of the invention to inhibit the Kv1.5 potassium channel was measured 5 in an electrophysiology experiment, using recombinant cells expressing the channel of interest in a whole cell patch clamp experiment.

The external bathing solution contained (in mM): 150 NaCI, 10 KCI, 3 MgCI₂, 1 CaCI₂,10 HEPES, pH 7.4. Patch pipettes were filled with an electrode solution of composition (in mM): 160 KCI, 0.5

MgCI₂,10 HEPES, 1 EGTA, pH 7.2 with KOH.

Compounds were dissolved in DMSO (100 %) and freshly made up in the external bather at the desired concentration (final DMSO concentration = 0.1%). Ail experiments were conducted at room température.

For whole-cell patch-clamp studies cells (CHO stably transfected with hKv1.5) were seeded onto glass coverslips before recordings were made. Cells were seeded in stérile 30 mm Pétri dishes at a density to enable isolated cells to be selected for patch clamp experiments. The dishes were stored in a humidified, gassed (5 % CO₂) incubator at 37 °C until use.

Whole-cell patch-clamp recordings of membrane currents were made following gigaohm seal formation between the patch electrode and the cell using HEKA EPC-9/10 amplifiers controlled by Puise Software (Ver8.5x/8.6x, HEKA, Germany). Coverslips seeded with cells were placed in a recordîng chamber mounted on the stage of an inverted microscope. During the experiment the cell of interest was continuously superfused with bather solution delivered via a cannula placed in close proximity to the cell to enable control of the extracellular solution environment. Only those cells with a current >500pA were used for experiments. During experiments total sériés résistance did not exceed 10 ΜΩ and was compensated by a minimum of 70 %. Leak subtraction was performed online using a P/n protocol in Puise.

Electrophysiology voltage-step protocols and analysis of data was performed as follows. Data was sampled at 5kHz, and filtered with a -3 dB bandwidth of 2.5kHz. Cells were held at a voltage of 80mV. Currents were evoked by a depolarisîng voltage step to OmV (900ms) before repolarisation first to -40mV (100ms) before returning to -80mV. The command waveform as repeatedly applied every 5s throughout the experiment. Mean currents during 75-95% of the depolarisîng step to OmV were analysed using Pulsefit software (v8.x, HEKA, Germany). The voltage protocol was applied a achieve a stable current baseline in bather before the test substance was superfused via the cannula; fluid exchange took approximately 15 s. The test substance was allowed to equilibrate during which time voltage protocol was repeatedly applied and recorded. Percentage inhibition of the current in the presence of test substance was calculated relative to the control pre-drug value.

	Compound	Kv1.5 IC50 (nM)
Racemate	1-{5-Phenyl-4-[(pyridin-2-ylmethyl)-amino]-thieno[2,3d]pyrimidin-2-yl)-piperidine-3carboxylic acid (2-hydroxyethyl)-amide	9
(S) enantiomer (Ib)	(S)-1-{5-Phenyl-4-[(pyridin-2-ylmethyl)-amino]thieno[2,3-d]pyrimidin-2-yl}-piperidine-3-carboxylic acid (2-hyd roxy-ethyl )-a m ide	27
(R)	(R)-1-{5-Phenyl-4-[(pyridin-2-ylmethyl)-amino)-	5

enantiomer (le) thienop.S-dJpyrïmidin^-ylJ-piperidine-S-carboxylïc acid (2-hyd roxy-eth y I )-a m i d e

Example 19

Selectivity screening

A compound of the invention and a comparative compound were screened in the following assays:

1. Nav1.5; screened on the Sophion QPatch using CHO cells expressing hNav1.5 currents, stably transfected with heterologous hNav1.5 cDNA.

2. Kv4.3; screened by manual whole cell patch clamp using CHO cells expressing hKv4.3 currents, stably transfected with heterologous Kv4.3 cDNA.

3. hERG; screened by manual whole cell patch clamp using HEK293 cells expressing hERG 10 currents, stably transfected with heterologous hERG cDNA.

4. Kir3.1/3.4; screened by manual whole cell patch clamp using HEK293 cells expressing rKir3.1/3.4 currents, stably transfected with heterologous rKir3.1 and rKir3.4 cDNA.

5. KCNQ1; screened by manual whole cell patch clamp using CHO cells expressing hKCNQI/hmink currents, stably transfected with heterologous hKCNQ1/hmink cDNA.

6. Kir2.1; screened by manual whole cell patch clamp using HEK293 cells expressing hKir21.

currents, stably transfected with heterologous hKir2.1 cDNA.

7. Cav1.2; screened using GH3 cells or HEK293 cells expressing hCav1.2 currents, stably transfected with heterologous hCav1.2 cDNA.

The selectivity ratios for Kv1.5 as compared to the above ion channels are shown below:

Ion Channel	Compound of the invention	Comparative compound
Nav1.5	>350x	~120x
Kv4.3	~500x	17x
hERG	-275x	54x
Kir3.1/3.4	~265x	~42x
KCNQ1	~1200x	~300x
Kir2.1	>400x	>1245x
Cav1.2	>1200x	>1245x

3l

Example 20

Inhibition of the l_Kur current in dissociated human atrial myocytes

Isolation of human atrial myocytes

Specimens of human atrial appendage (either right or left) were obtained from patients undergoing a range of cardiac surgical procedures. Tissue was obtained from consenting patients from Papworth Hospital NHS Trust, Cambs. UK. following approval from the Local Research Ethical Approval Committee, The mechano-enzymatic isolation of myocytes was performed using a modified protocol as described by Wang et al. (1993) and Dobrev ef al. (2005). Isolated myocytes were suspended in a modified 'Krafte-brühe' (KB) solution until use.

Recording system

Myocytes were placed into a small-volume recording chamber with a glass-coverslip base, mounted on the stage of an inverted microscope. During the experiment, the cell of interest was constantly superfused with bather solution delivered via a cannula placed in close proximity to the cell to enable control of the extracellular solution environment. Whole-cell patch-clamp recordings of membrane currents were made using a HEKA EPC-9/10 amplifier following Gigaohm seal formation between the patch electrode and the myocyte. Glass patch-pipettes were pulled from borosilicate glass. Only rod-shaped, striated myocytes were selected for use. Capacitance and sériés résistance were compensated using Puise software. Voltage-clamp commands were generated using Puise software and data were recorded onto the hard disk of a PC. Leak subtraction was not performed and cells with significant leak were rejected. Experiments were performed at room température. To minimise contamination from other ionic currents, experimental solutions contained 10 mM tetraethylammonium chloride (l_K), 100 nM atropine (Ικ.αοη), 200 μΜ CdCI₂ (l_Ca<L; and l_Ci.ca). 0-5 mM BaCI₂ (l_Ki and Ικαοπ)- Blockers were used at a concentration that would not be expected to affect l_Kur· The sodium current (l_Na) was suppressed by using a choline chloride based bather. Depolarising voltage-step were applied every 10s to elicit an outward potassium current composed of a transient and sustained component. The sustained current sensitive to 300 μΜ 4-AP was defined as the ultra-rapid delayed rectifier current, l_Kur·

Ionic current	Compound of the invention	Comparative compound
hlfcur	11 nM	154 nM

Abbreviations

HGNC	HUGO Gene Nomenclature Committee
KV(Ur)	Cardiac Ultrarapid Delayed Rectifier
CHO	Chinese Hamster Ovary Cells
IP3	Inositol Triphosphate
CRAC	Ca2+-Release-Activated-Ca2+ Current

DMEM Dulbecco’s Modified Eagle media

DMSO	Dimethyl sulphoxide
FCS	Fêtai Calf Sérum
EBSS	Earls Balanced Sait Solution
WCPC	Whole-Cell Patch-Clamp
HEK293	Human Embryonic Kidney 293 Cells

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Claims (13)

1. A compound of formula (la) or a pharmaceutically acceptable ester or sait thereof.

10

2. The compound of claim 1, wherein the compound is of formula (lb)

3. The compound of claim 1, wherein the compound is of formula (le) (le).

4. The compound of claim 1, wherein the compound of formula (la) comprises a mixture of the compounds of formulae (lb) and (le).

5. The compound of claim 4, wherein the compound of formula (la) comprises a racemic mixture ofthe compounds of formulae (lb) and (le).

6. The compound of claim 4, wherein the compound of formula (la) comprises an enantiomeric excess of the compound of formula (lb).

7. The compound of claim 4, wherein the compound of formula (la) comprises an enantiomeric excess ofthe compound offormula (le).

8. A pharmaceutical composition comprising at least one compound as claimed in any one of claims 1 to 7 and, optionally, one or more pharmaceutically acceptable excipients.

9. A compound or composition as claimed in any one of claims 1 to 8 for use in therapy.

10. A compound or composition as claimed in any one of claims 1 to 8 for use in potassium channel inhibition.

11. The compound or composition as claimed in claim 10, wherein the compound or composition is for use in the treatment or prévention of arrhythmia.

12. The use of a compound as claimed in any one of claims 1 to 7 for the manufacture of a médicament for use in potassium channel inhibition.

13. The use of claim 12 wherein the médicament is for use in the treatment or prévention of arrhythmia.