OA16585A - Combination therapies for hematologic malignancies. - Google Patents

Abstract

The invention provides methods that relate to a novel therapeutic strategy for the treatment of hematological malignancies and inflammatory diseases. In particular, the method comprises administration of a compound of formula A wherein R is H, halo, or C1-C6 alkyl; R' is C1-C6 alkyl; or a pharmaceutically acceptable salt thereof; and optionally a pharmaceutically acceptable excipient; and one or more additional therapeutic agents optionally selected from the group consisting of bendamustine, rituximab, and ofatumumab.

Description

COMBINATION THERAPIES FOR HEMATOLOGIC MALIGNANCIES

Cross-Reference to Related Applications [0001] This application daims priority from U.S. Provisionai Patent Application

Nos. 61/452,034 filed on March 11, 2011 and 61/493,317 filed on Jun 3, 2011, both of which are 5 incorporated by reference in their entirety.

Technical Field [0002] The présent application is in the field of therapeutics and médicinal chemistry. In particular, the présent application concerns uses of certain quinazoline dérivatives in combination with other therapeutic treatments to treat hématologie malignancies and certain 10 other conditions.

Back ground Art [0003] Cell signaling via 3’-phosphorylated phosphoinositides has been implicated in a variety of cellular processes, e.g., malignant transformation, growth factor signaling, inflammation, and immunity. The enzyme responsible for generating these phosphorylated 15 signaling products, phosphatidylinositol 3-kinase (PI 3-kinase; PI3K), was originally identified as an activity associated with viral oncoproteins and growth factor receptor tyrosine kinases that phosphorylâtes phosphatidylinositol (PI) and its phosphorylated dérivatives at the 3’-hydroxyl of the inositol ring.

[0004] PI 3-kinase activation, is believed to be involved in a range of cellular responses încluding cell growth, différentiation, and apoptosis.

[0005] The initial purification and molecular cloning of PI 3-kinase revealed that it was a heterodimer consisting of p85 and pllO subunits. Four distinct Class I PI3Ks hâve been identified, designated PI3K α, β, 6, and γ, each consisting of a distinct 110 kDa catalytic subunit and a regulatory subunit. More specifically, three of the catalytic subunits, Le., pl 10a, pl 10β and pl 105, each interact with the same regulatory subunit, p85; whereas pl 10γ interacts with a distinct regulatory subunit, plOl. The patterns of expression of each of these PI3Ks in human cells and tissues are also distinct.

[0006] Identification of the p 110δ isoform of PI 3-kinase is described in Chantry et al., J Biol Chem, 272:19236-41 (1997). It was observed that the human pl 10δ isoform is expressed in a tissue-restricted fashion. It is expressed at high levels in lymphocytes and lymphoid tissues, suggesting that the protein might play a rôle in PI 3-kinase-mediated signaling in the immune system. The pl 10β isoform of PI3K may also play a rôle in PI3K-mcdiated signaling in certain cancers.

[0007] There is a need for a treatment relating to PI3K-mediated disorders relating to cancers, inflammatory diseases, and autoimmune diseases. Specifically, there is a need for treatment of cancers that are hématologie malignancies, such as leukemia and lymphoma.

[0008] Existing chemoimmunotherapy approaches to chronic lymphocytic leukemia (CLL) can reduce prolifération and enhance apoptosis of the malignant lymphocytes; however, these salutary effects may be limited in lymph nodes because existing drugs may not penetrate effectively to these sites and because non-malignant stromal cells provide support to the malignant lymphocytes.

[0009] Existing chemotherapies or immunothérapies for CLL include cyclophosphamide, chlorambucil, fludarabine, rituximab, alemtuzumab, and ofatumumab. These thérapies are particularîy effective at killing circulating malignant lymphocytes, but can be less effective in reducing malignant lymphadenopathy. Moreover, these thérapies do not induce lymphocyte redistribution or cause lymphocytosis.

[0010] What is needed in the art to treat patients with CLL is a treatment that can modulate chemotactic signaling between lymphocytes and stromal cells. Furthermore, what is needed is a treatment that can reduce such signaling results to redistribute malignant lymphocytes from the lymph nodes into the circulation. Such redistribution would permit better killing of these cells by a chemotherapeutic agent or an immunotherapeutic agent.

Summary [0011] The présent application meets the need in the art by providing a method to use these compounds in combination with other chemotherapies or immunothérapies to treat cancer, inflammatory, and autoimmune diseases. In particular, cancers that are hématologie malignancies, such as leukemia and lymphoma, are treated by the methods described herein.

[0012] In one aspect, provided is a method to treat cancer, comprising administering to a subject in need of such treatment, an effective amount of a compound of formula A, xZ

wherein R is H, halo, or C1-C6 alkyl; R’ is C1-C6 alkyl; or a pharmaceutically acceptable sait thereof; and optionally a pharmaceutically acceptable excipient; and one or more additional therapeutic agents optionally selected from the group consisting of bendamustine, rituximab, and ofatumumab.

[0013] In one embodiment, the compound is

[0014] In another embodiment, the compound is

[0015] In some of the foregoing embodiments, the cancer is a hematological malignancy. [0016] In some of the foregoing embodiments, the hematological malignancy is B-cell malignancy.

[0017] In some of the foregoing embodiments, the hematological malignancy is leukemia or 15 lymphoma.

[0018] In some of the foregoing embodiments, the cancer is chronic lymphocytic leukemia (CLL) or non-Hodgkin’s lymphoma (NHL).

[0019] In some embodiments, the cancer is indolent non-Hodgkin’s lymphoma (ÎNHL). [0020] In other embodiments, the compound and the one or more therapeutic agents are each administered at least once during at least one cycle, and wherein the one or more therapeutic agents are administered to the subject in the same or different cycle as the administration of the compound.

[0021] In some of the foregoing embodiments, the cycle is 7 to 42 days.

[0022] In some of the foregoing embodiments, the one or more therapeutic agents are administered to the subject on at least the first and second days of at least one cycle.

[0023] In some of the foregoing embodiments, the one or more therapeutic agents are administered weekly to the subject during at least one cycle.

[0024] In some of the foregoing embodiments, between 50 mg and 200 mg of the compound is administered to the subject twice per day.

* [0025] In some of the foregoing embodiments, between 50 and 1,500 mg/m of the one or more therapeutic agents is administered to the subject.

[0026] In some of the foregoing embodiments, the compound is présent in a pharmaceutical composition comprising the compound, and at least one pharmaceutically acceptable excipient. [0027] In some of the foregoing embodiments, the subject is résistant to standard chemotherapeutic treatments.

[0028] In some of the foregoing embodiments, the subject has at least one enlarged lymph node.

[0029] In some of the foregoing embodiments, the subject is i) refractory to at least one chemotherapy treatment, or ii) is in relapse after treatment with chemotherapy, or a combination thereof.

[0030] In another aspect, provided is a method to treat cancer, comprising administering to a subject în need of such treatment, an effective amount of a compound of formula A,

(Formula A),

wherein R is H, halo, or C1-C6 alkyl; R’ is C1-C6 alkyl; or a pharmaceutically acceptable sait thereof; and optionally a pharmaceutically acceptable excipient; and one or more additional therapeutic agents optionally selected from the group consisting of bendamustine, rituximab, ofatumumab, and lenalidomide.

[0031] In yet another aspect, provided is a method to treat a condition, comprising administering to a subject in need of such treatment, an effective amount of a compound of formula I” or formula II”,

N

NH

NH or a pharmaceutically acceptable sait thereof, and one or more additional therapeutic agents optionally selected from the group consisting of bendamustine, rituximab, and ofatumumab, wherein the condition is chronic lymphocytic leukemia (CLL) or indolent non-Hodgkin’s lymphoma (iNHL).

[0032] In one embodiment, the subject is i) refractory to at least one chemotherapy treatment, or ii) is in relapse after treatment with chemotherapy, or a combination thereof.

[0033] In some of the foregoing embodiments, the compound and the one or more therapeutic agents are each administered at least once during at least one cycle, and wherein the one or more therapeutic agents are administered to the subject in the same or different cycle as the administration of the compound.

[0034] In some of the foregoing embodiments, the cycle is 7 to 42 days.

[0035] In some of the foregoing embodiments, between 50 mg and 200 mg of the compound is administered to the subject twice per day.

[0036] In one embodiment, the one or more therapeutic agents is bendamustine.

[0037] In some of the foregoing embodiments, the bendamustine is administered to the subject on at least the first and second days of at least one cycle.

[0038] In some of the foregoing embodiments, the bendamustine is administered for at least 6 cycles.

[0039] In some of the foregoing embodiments, each dose of the bendamustine is between 50 mg/m² and 150 mg/m².

[0040] In some of the foregoing embodiments, the method further comprises admînistering rituximab to the subject.

[0041] In some of the foregoing embodiments, the rituximab is administered on the first day of each cycle for at least 6 cycles.

[0042] In another embodiment, the one or more therapeutic agents is rituximab.

[0043] In some of the foregoing embodiments, the rituximab is administered weekly to the subject during at least one cycle.

[0044] In some of the foregoing embodiments, each dose of the rituximab is between 300 mg/m² and 400 mg/m².

[0045] In yet another embodiment, the one or more therapeutic agents is ofatumumab.

[0046] In some of the foregoing embodiments, at least 12 doses of ofatumumab are administered over 6 cycles.

[0047] In yet another aspect, provided is a method to treat a condition, comprising admînistering to a subject in need of such treatment, an effective amount of a compound of formula I” or formula II”,

N or a pharmaceutically acceptable sait thereof, and one or more additional therapeutic agents optionally selected from the group consisting of bendamustine, rituximab, ofatumumab, and lenalidomide, [0048] In one embodiment, the one or more therapeutic agents is lenalidomide.

[0049] In yet another aspect, provided is a method of treating a subject with a B-cell disorder, comprising:

a) identifying a subject having a B-celI malignancy, wherein the subject is refractory to or is in relapse after at least one or more treatments selected from the group consisting of bortezomib (Velcade®), carfilzomib (PR-171), PR-047, disulfiram, lactacystin, PS-519, eponemycin, epoxomycin, aclacinomycin, CEP-1612, MG-132, CVT-63417, PS-341, vinyl sulfone tripeptide inhibitors, ritonavir, PI-083, (+/-)-7-methylomuralide, (-)-7-methylomuralide, perifosine, rituximab, sildenafil citrate (Viagra®), CC-5103, thalidomide, epratuzumab (hLL2anti-CD22 humanized antibody), simvastatin, enzastaurin, Campath IH®, dexaméthasone, DT PACE, oblimersen, antineoplaston A10, antineoplaston AS2 1, alemtuzumab, beta alethine, cyclophosphamide, doxorubicin hydrochloride, PEGylated liposomal doxorubicin hydrochloride, prednisone, prednisolone, cladribine, vincristine sulfate, fludarabine, filgrastim, melphalan, recombinant interferon alfa, carmustine, cisplatin, cyclophosphamide, cytarabine, etoposide, melphalan, dolastatin 10, indium In 111 monoclonal antibody MN-14, yttrium Y 90 humanized epratuzumab, anti-thymocyte globulin, busulfan, cyclosporine, methotrexate, mycophenolate mofetil, therapeutic allogeneic lymphocytes, Yttrium Y 90 ibritumomab tiuxetan, sirolimus, tacrolimus, carboplatin, thiotepa, paclitaxel, aldesleukin, recombinant interferon alfa, docetaxel, ifosfamide, mesna, recombinant interleukin-12, recombinant interleukin-11, Bcl-2 family protein inhibitor ABT-263, denileukin diftitox, tanespimycin, everolimus, pegfilgrastim, vorinostat, alvocidib, recombinant flt3 ligand, recombinant human thrombopoietin, lymphokine-activated killer cells, amifostine trihydrate, aminocamptothecin, irinotecan hydrochloride, caspofungîn acetate, clofarabine, epoetin alfa, nelarabine, pentostatin, sargramostim, vinorelbine ditartrate, WT-1 analog peptide vaccine, WT1 126-134 peptide vaccine, fenretinide, ixabepilone, oxaliplatin, monoclonal antibody CD19, monoclonal antibody CD20, omega-3 fatty acids, mitoxantrone hydrochloride, octreotide acetate, tositumomab and iodine 1131 tositumomab, motexafin gadolinium, arsenic trioxide, tipifarnib, autologous human tumor-derived HSPPC-96, veltuzumab, bryostatin 1, anti-CD20 monoclonal antibodies, chlorambucil, pentostatin, lumiliximab, apolizumab, Anti-CD40, ofatumumab, temsirolimus, bendamustine, purine analogs, lenalidomide, an alkylating agent, and anthracycline-containing therapy, or a combination thereof;

b) administering to a subject in need of such treatment, a compound of formula I

or a pharmaceutically acceptable sait thereof; and optionally a pharmaceutically acceptable excipient; and one or more additional therapeutic agents optionally selected from the group consisting of bendamustine, rituximab, lenalidomide and ofatumumab.

[0050] In one embodiment, the subject is refractory or is in relapse after at least one or more treatments selected from the group consisting of rituximab, an alkylating agent, fludarabîne, and an anthracycline-containing therapy, and a combination thereof.

[0051] In some of the foregoing embodiments, between 50 mg and 200 mg of the compound is adminîstered to the subject twice per day during at least one or more cycles.

[0052] In some of the foregoing embodiments, between 50 mg/m² and 1,500 mg/m² of the one or more additional therapeutic agents is adminîstered to the subject at least once during at least one or more cycles, wherein the one or more therapeutic agents are adminîstered to the subject in the same or different cycle as the administration of the compound.

[0053] In yet another aspect, provided is a method of treating a subject with a B-cell disorder, comprising:

a) identifying a subject having a B-cell malignancy, wherein the subject is refractory to or is in relapse after at least one or more treatments selected from the group consisting of bortezomib (Velcade®), carfilzomib (PR-171), PR-047, disulfiram, lactacystin, PS-519, eponemycin, epoxomycin, aclacinomycin, CEP-1612, MG-132, CVT-63417, PS-341, vinyl sulfone tripeptide inhibitors, ritonavir, PI-083, (+/-)-7-methylomuralide, (-)-7-methylomuralide, perifosine, rituximab, sildenafil citrate (Viagra®), CC-5103, thalidomîde, epratuzumab (hLL2anti-CD22 humanized antibody), simvastatin, enzastaurin, Campath IH®, dexamethasone, DT PACE, oblimersen, antineoplaston A10, antineoplaston AS2 1, alemtuzumab, beta alethine, cyclophosphamide, doxorubicin hydrochloride, PEGylated liposomal doxorubicin hydrochloride, prednisone, prednisoione, cladribine, vincristine sulfate, fludarabîne, filgrastim, melphalan, recombinant interferon alfa, carmustine, cisplatin, cyclophosphamide, cytarabine,

etoposide, melphalan, dolastatin 10, indium In 111 monoclonal antibody MN-14, yttrium Y 90 humanized epratuzumab, anti-thymocyte globulin, busulfan, cyclosporine, methotrexate, mycophenolate mofetil, therapeutic allogeneic lymphocytes, Yttrium Y 90 ibritumomab tiuxetan, siroiimus, tacrolimus, carboplatin, thiotepa, paclitaxel, aldesleukin, recombinant interferon alfa, docetaxel, ifosfamide, mesna, recombinant interleukin-12, recombinant interleukin-11, Bcl-2 family protein inhibitor ABT-263, denileukîn diftitox, tanespimycin, everolimus, pegfilgrastim, vorinostat, alvocidib, recombinant flt3 ligand, recombinant human thrombopoietin, lymphokine-activated killer cells, amifostine trihydrate, aminocamptothecin, irinotecan hydrochloride, caspofungin acetate, clofarabine, epoetin alfa, nelarabine, pentostatin, sargramostim, vinorelbine ditartrate, WT-1 analog peptide vaccine, WT1 126-134 peptide vaccine, fenretinide, ixabepîlone, oxaliplatin, monoclonal antibody CD19, monoclonal antibody CD20, omega-3 fatty acids, mitoxantrone hydrochloride, octreotide acetate, tositumomab and iodine 1131 tositumomab, motexafin gadolinium, arsenic trioxide, tipifarnib, autologous human tumor-derived HSPPC-96, velluzumab, bryostatin 1, anti-CD20 monoclonal antibodies, chlorambucil, pentostatin, lumiliximab, apolizumab, Anti-CD40, ofatumumab, temsirolimus, bendamustine, purine analogs, lenalidomide, an alkylating agent, and anthracycline-containing therapy, or a combination thereof;

b) administering to a subject in need of such treatment, a compound of formula I

or a pharmaceutically acceptable sait thereof; and optionally a pharmaceutically acceptable excipient; and one or more additional therapeutic agents optionally selected from the group consisting of bendamustine, rituximab, ofatumumab, and lenalidomide.

[0054] In another aspect, the présent disclosure provides a method to treat chronic lymphocytic leukemia (CLL) or indolent non-Hodgkin’s lymphoma (iNHL), comprising

administering to a subject in need of such treatment, an effective amount of a compound of formula I,

or a pharmaceutically acceptable sait thereof;

optionally a pharmaceutically acceptable excipient; and bendamustine.

[0055] In one embodiment, the compound and bendamustine are each administered at least once during at least one cycle, wherein the bendamustine is administered to the subject in the same or different cycle as the administration of the compound, [0056] In some of the foregoing embodiments, the cycle is 7 to 42 days.

[0057] In some of the foregoing embodiments, the compound is administered to the subject twice per day during at least one cycle, and wherein the bendamustine is administered to the subject on at least the first and second days of at least one cycle.

[0058] In some of the foregoing embodiments, the bendamustine is administered for at least 6 cycles.

[0059] In some of the foregoing embodiments, a dose of the compound is between 50 mg and 200 mg, and wherein a dose of bendamustine is between 50 mg/m² and 150 mg/m².

[0060] In some of the foregoing embodiments, the method further comprises administering one or more additional therapeutic agents selected from the group consisting of rituximab and ofatumumab.

[0061] In some of the foregoing embodiments, the method further comprises administering one or more additional therapeutic agents selected from the group consisting of rituximab, ofatumumab, and lenalidomide.

[0062] In yet another aspect, provided is a method to treat chronic lymphocytic leukemia (CLL) or indolent non-Hodgkin’s lymphoma (iNHL), comprising administering to a subject in need of such treatment, an effective amount of a compound of formula I,

or a pharmaceutically acceptable sait thereof;

optionally a pharmaceutically acceptable excipient; and rituximab.

[0063] In one embodiment, the compound and rituximab are each administered at least once during a cycle, and wherein the rituximab is administered to the subject in the same or different cycle as the administration of the compound.

[0064] In some of the foregoing embodiments, the cycle is 7 to 42 days.

[0065] In some of the foregoing embodiments, the compound is administered to the subject twice per day during at least one cycle, and wherein the rituximab is administered weekly to the subject during at least one cycle.

[0066] In some of the foregoing embodiments, a dose of the compound is between 50 mg and

200 mg, and wherein a dose of the rituximab is between 300 mg/m² and 400 mg/m².

[0067] In some of the foregoing embodiments, the method further comprises administering one or more additional therapeutic agents selected from the group consisting of bendamustine and ofatumumab.

[0068] In some of the foregoing embodiments, the method further comprises administering one or more additional therapeutic agents selected from the group consisting of bendamustine, ofatumumab, and lenalidomide.

[0069] In yet another aspect, provided is a method to treat chronic lymphocytic leukemia (CLL) or indolent non-Hodgkin’s lymphoma (iNHL), comprising administering to a subject in need of such treatment, an effective amount of a compound of formula I,

or a pharmaceutically acceptable sait thereof;

optionally a pharmaceutically acceptable excipient; and lenalidomide.

[0070] In one embodiment, the compound and lenalidomide are each administered at least once during a cycle, and wherein the lenalidomide is administered to the subject in the same or different cycle as the administration of the compound.

[0071] In yet another aspect, provided is a method to treat chronic lymphocytic leukemia (CLL) or indolent non-Hodgkin’s lymphoma (iNHL), comprising administering to a subject in 10 need of such treatment, an effective amount of a compound of formula I,

or a pharmaceutically acceptable sait thereof;

optionally a pharmaceutically acceptable excipient; and ofatumumab.

[0072] In some of the foregoing embodiments, the dose of the compound is between 50 mg and 200 mg, and the dose of ofatumumab is between 200 mg and 1500 mg. In some of the foregoing embodiments, an initial dose of ofatumumab is administered which is different from subséquent doses of ofatumumab.

Brief Description of the Drawings [0073] Figure 1 shows a graphical summary of multiple myeloma (MM) cell growth as a function of varying concentrations of cytokines IGF-1 and IL-6 in combination with compound I, using LB cells.

[0074] Figure 2 shows a graphical summary of cell growth of multiple myeloma (MM) cells as a function of varying concentrations of compound I and the presence or absence of bone marrow stromal cells (BMSC) after 48 hours.

[0075] Figure 3 shows a graphical summary of apoptosis of Chronic Lymphocytic Leukemia (CLL) cells as a function of varying concentrations of compound of formula I.

[0076] Figure 4 shows a summary chart of the effect of compound I on cell viability, réduction in Akt (Ser473) phosphorylation, and caspase 3 activation in several different Acute Lymphoblastîc Leukemia (ALL) cell lines.

[0077] Figure 5 shows a summary of the effect of compound I on the cell cycle of acute lymphoblastîc leukemia (ALL) cell lines.

[0078] Figure 6 shows a graphical summary of the effect of varying concentration of compound I on cellular growth in breast cancer T47D and HS-578T cell lines at 48 hrs and 72 hrs.

[0079] Figure 7 shows a graphical summary of the effect of varying concentrations of compound I on cellular growth of ovarian IGROV-1 and OVCAR-3 cell lines at 48 hrs 20 and 72 hrs.

[0080] Figure 8 shows a summary of the effect of compound I on Akt phosphorylation in many leukemia and lymphoma cell lines.

[0081] Figure 9 shows SDS-PAGE images and displays of Akt and pAkt in various hematopoietic cancer cell lines as a function of the presence or absence of compound I, showing 25 compound I inhibits Akt phosphorylation.

[0082] Figure 10 shows graphical summaries of apoptotic and viable cell populations in breast cancer cell lines as a function of varying concentrations of compound formula I, demonstrating that the compound induces apoptosis.

[0083] Figure 11 shows the concentration of compound I in the blood of a healthy human subject over 12 hours after oral administration of 50, 100 and 200 mg doses of said compound.

vZ

[0084] Figure 12 shows the comparison of lésion areas in a human patient diagnosed with mande cell lymphoma after 28 days (1 cycle) of treatment with compound I and lésion areas prior to treatment.

[0085] Figure 13 shows the ALC (absolute lymphocyte count) in the blood of a patient over a period of 4 weeks after 28 days (1 cycle) of treatment with the compound of formula I.

[0086] Figure 14 shows the concentration of compound I in the blood of patients with and without mande cell lymphoma (MCL) over 6 hours after administration (50 mg BID) at day 28, compared to the concentration in the blood of a normal healthy volunteer at day 7 (D7) using the same dosing schedule or dosing with 100 mg BID of Compound I.

[0087] Figure 15 shows PI3K isoform expression in a panel of lymphoma and leukemia cell lines.

[0088] Figure 16A shows cell viability and apoptosis data in leukemia cell lines exposed to Compound I. In Figure 16B the Annexin staining indicates an increase in apoptosis in the treated cells.

[0089] Figures 17A-D shows PAGE results of different PI3K isoform expression in CLL patient cells.

[0090] Figure 18 shows the induction of (A) caspase 3 and (B) PARP cleavage in the presence of compound I.

[0091] Figure 19 shows increased apoptosis of Chronic Lymphocytic Leukemia (CLL) cells from poor prognosis patients caused by exposure to compound I, demonstrating that compound I is effective in drug résistant patients.

[0092] Figure 20 shows increased apoptosis of Chronic Lymphocytic Leukemia (CLL) cells from refractory/ relapsed patients caused by exposure to the compound of formula I.

[0093] Figure 21 shows the results of Phospho-Akt production in the absence or presence of 0.1,1.0,10 μΜ of compound I.

[0094] Figure 22 shows flow cytometry results relating to PI3K signaling in basophils, demonstrating that (B) anti-FCeRl or (C) fMLP încreases CD63 expression compared to no stimulation (A).

[0095] Figure 23 shows inhibition of PI3K inhibition by compound I in basophils, and demonstrates that Compound I is especially effective at inhibition of CD63 expression induced by a pl 10δ pathway, but also effective at micromolar concentration to inhibït expression induced by a pl 10γ pathway.

[0096] Figure 24 shows pharmacokinetic data of (A) single dose administration of compound I at different dose amounts in healthy volunteers, and (B) a pharmacokinetic profile that maintains an effective dosage over a 12 hour period.

[0097] Figure 25 shows the effects of various doses of compound I on (A) glucose and (B) insulin levels, exhibiting little off-target activity.

[0098] Figure 26A shows the PI3K isoform expression in a panel of DLBCL cell lines.

[0099] Figure 26 B shows an SDS-PAGE image of pAkt in DLBCL cell lines in the presence or absence of compound I.

[0100] Figure 27 shows the effects of a 10 μΜ concentration of compound I on the phosphorylation of Akt and S6 in ALL cell lines in SDS-PAGE.

[0101] Figure 28 shows a dose dépendent réduction of phosphorylation of Akt, S6, and GSK3β after treatment with a sériés of compound I dilutions.

[0102] Figure 29 shows dose dépendent effects of compound I on ALL cell lines in the downregulation of cFLIP, cleavage of Caspase 3, and cleavage of PARP.

[0103] Figure 30 shows expression of pllO delta in A) MM cell lines and B) patient MM cells; and C) in MM.1S and LB cells.

[0104] Figure 31A shows expression of pllO delta from LB and INA-6 cells transfected with pllO delta siRNA (Si) or control siRNA (mock).

[0105] Figure 31B shows a graph of INA-6 cell growth after transfection with pllO delta siRNA (Si) or control siRNA (mock).

[0106] Figure 31C shows the % of viable cells cultured with or without compound I for 48 hours.

[0107] Figure 31D shows the % of viable MM cells after being cultured with compound I at concentrations from 0 to 20 μΜ for 48 hours.

[0108] Figure 31E shows the % of viable peripheral blood mononuclear cells from healthy donors after being cultured with compound I at various concentrations for 72 hours.

[0109] Figure 31F shows immunoblotting results of lysâtes from INA-6 cells cultured with compound I (0-5 μΜ) for 120 hours.

[0110] Figure 32 shows immunoblot AKT and ERK expression profiles after culturing of A) 30 INA-6 cells with compound I or LY294002 for 12 hours; B) INA-6 and MM.1S cells with compound I at various concentrations for 6 hours; C) LB and INA-6 cells with compound I for 0-6 hours.

[OUI] Figure 33A shows fluorescent and transmission électron microscopie images of INA-6 and LB MM cells treated with compound I for 6 hours and LC3 accumulation; arrows indicate autophagosomes.

[0112] Figure 33B shows fluorescence microscopy images of INA-6 cells treated with 5 μΜ of compound I or sérum starvation for 6 hours.

[0113] Figure 33C shows immunoblots of LC3 and beclin-1 protein levels from INA-6 cells treated with or without compound I and 3-MA (3-methyl adenine, a known inhibitor of autophagy).

[0114] Figure 33D shows % growth of pl 10δ positive LB cells after treatment with up to

100 μΜ of 3-MA for 24 hours.

[0115] Figure 34 shows the levels of growth inhibition of A) LB or B) INA-6 cells co-cultured with 0,5, and 10 μΜ of compound I in the presence or absence of varying amounts of IL-6 or IGF-1; Legend: control media ( ); compound I at 5.0 μΜ ( ) or 10 μΜ ( O ).

[0116] Figure 34C and 34D show MM cell growth inhibition in the presence of BMSC.

Legend for 34C only: control media (D), Compound 12.5 μΜ (®), 5 μΜ (®), and 10 μΜ ( ). [0117] Figure 34 E shows immunoblots of IL-6 in culture supernatants from BMSCs cultured with compound I or control media for 48 hours.

[0118] Figure 34F shows immunoblots of AKT and ERK expression profiles in INA-6 cells treated with compound I cultured with our without BMSCs.

[0119] Figure 34G shows % BMSC cell growth in two different patients after culturing with compound I for 48 hours.

[0120] Figure 35A shows microscopie images of HuVECs (human umbilical vein endothélial cells) cultured with 0,1 and 10 μΜ of compound I for 8 hours and microtubule formation assessed.

[0121] Figure 35B shows a bar chart summarizing endothélial cell tube formation in HuVEC cells treated with compound I.

[0122] Figure 35 C shows a graph charting % cell growth of HuVECs as a function of the increasing culture concentration of compound I.

[0123] Figure 35 D shows decreasing Akt and ERK expression of HuVEC cell lysâtes after being cultured with compound I for 8 hours.

[0124] Figure 36A charts the tumor volume in SCID mice with human MM xenografts treated with 0,10 mg/kg or 30 mg/kg of compound II as a function of time, showing strong In vivo activity on the human xenograft tumor [0125] Figure 36 B shows a photograph comparing the tumor from human MM xenografts on 5 a mouse treated with compound II for 12 days to a control mouse.

[0126] Figure 36C shows the survival rate of SCID mice with human MM xenografts treated with 0,10, and 30 mg/kg compound II over time.

[0127] Figure 36D shows images from immuno-histochemistric analysis of tumors harvested from a mouse treated with compound II in comparison to the control; wherein CD31 and P-AKT 10 positive cells are dark brown.

[0128] Figure 36E shows immunoblots detecting PDK-1 and AKT levels from tumor tissues harvested from mice treated with compound II in comparison to a control.

[0129] Figure 36F shows a chart of sIL6R levels measured in mice treated with 0,10 mg/kg or 30 mg/kg of compound II over a period of 4 weeks of treatment as determined by ELISA.

[0130] Figure 37A show the % of viable LB or INA-6 MM cells after treatment with compound I with varying amounts of bortezomib (B); Legend: medium (), compound I 1.25 μΜ (), 2.5 μΜ (M), or 5.0 μΜ (□).

[0131] Figure 37B shows immunoblots comparing levels of phosphorylation of AKT in INA-6 cells treated for 6 hours with compound I and/or bortezomib.

[0132] Figure 38 shows (A) PI3K isoform expression in a panel of follicular lymphoma cell lines; (B) réduction in the expression of pAkt, Akt, pS6 and S6 after exposure to compound I; and (C)Increase in PARP and caspase-3 cleavage after exposure to compound I in a dosedependent manner.

[0133] Figure 39 shows (A) amounts of constitutive PI3K signaling in primary MCL cells in various amounts of compound I; (B) réduction in pAkt production in MCL cell lines containing a survival factor and varying amounts of compound I.

[0134] Figure 40 show a computer tomography axillary image of a bulky lymphadenopathy in a patient with CLL (A) before treatment with compound I and (B) after 1 cycle of treatment with compound I.

[0135] Figure 41 shows that constitutive activation of the PI3KÔ pathway drives prolifération, survival, and abnormal trafficking of malignant Β-cells in iNHL and CLL.

[0136] Figure 42 shows a table summarizing patient characteristics and treatment disposition in a study involving 20 patients with indolent non-Hodgkin lymphoma (iNHL) and chronic lymphocytic leukemia (CLL).

[0137] Figure 43 shows a table summarizing the safety profile in a study where a compound of formula I was administered in combination with bendamustine (B) or rituximab (R) to patients with iNHL or CLL.

[0138] Figure 44 shows a graphical summary of the effîcacy of administering a compound of formula I in combination with bendamustine (B) or rituximab (R) to patients with iNHL or CLL. [0139] Figure 45 shows a table summarizing the response rates in patients with iNHL or CLL upon administering a compound of formula I in combination with bendamustine (B) or rituximab (R).

[0140] Figure 46 shows a graphical summary of lymphocyte counts in a study where only a compound of formula I was administered as a single agent to patients with CLL.

[0141] Figure 47 shows a graphical summary of lymphocyte counts in a study where a compound of formula I in combination with bendamustine (B) or rituximab (R) was administered to patients with CLL.

[0142] Figure 48A shows the contour plots depicting CLL cell viability after treatment with a compound of formula I, bendamustine, or the two drugs combined. Figure 48B shows a graph depicting the mean relative viabilities of CLL cells treated with a compound of formula I (5 μΜ), bendamustine (10 μΜ), or the drug combination (mean ± SEM, n=4), [0143] Figure 49A shows a graphical summary of PI3K-Ô enzymatîc activiy in cells as a function of exposure to lenalidomide and varying amounts of a compound of formula I.

[0144] Figures 49B and 49C show images of immunoblot assays showing the effects of lenalidomide and/or a compound of formula I on phosphorylation in CD19+ cells.

[0145] Figures 49D and 49 E show images of immunoblot assays showing the effects of transfected siRNA or nonsense siRNA on protein expression in CD19+ cells.

[0146] Figure 50A show a graphical summary of the effect of lenalidomide and/or a compound of formula I on the surface expression of CD40, CD86 in CD19+ cells of CLL patients.

[0147] Figure 50B shows a graphical summary of the effect of lenalidomide and/or a compound of formula I on mRNA expression of CD40, CD86, and CD154 in CD19+ cells of CLL patients.

[0148] Figure 50C shows a graphical summary of the effect of lenalidomide and/or a compound of formula I on the CD20 in CD19+ cells of CLL patients.

[0149] Figure 50D shows a graphical summary of the effect of lenalidomide and/or a compound of formula I on IgM concentration in CD19+ cells of CLL patients.

[0150] Figures 50E and 50F show a graphical summary of the effect of lenalidomide and/or a compound of formula I on cytokine mRNA expression in CD19+ cells of CLL patients.

[0151] Figure 51 shows a table summarizing patient characteristics and treatment disposition in a study involving 49 patients with indolent non-Hodgkin lymphoma (iNHL) and chronic lymphocytic leukemia (CLL).

[0152] Figure 52 shows a table summarizing the safety profile in a study where a compound of formula I was administered in combination with bendamustine (B) or rituximab (R) to patients with iNHL or CLL.

[0153] Figure 53A shows a graphical summary of the efficacy of administering a compound of formula I in combination with bendamustine (B) or rituximab (R) to patients with iNHL.

[0154] Figure 53B shows a graphical summary of the efficacy of administering a compound of formula I in combination with bendamustine (B) or rituximab (R) to patients with CLL.

[0155] Figure 54 shows a table summarizing the response rates in patients with iNHL or CLL upon administering a compound of formula I in combination with bendamustine (B) or rituximab (R).

[0156] Figure 55 shows a graphical summary of lymphocyte counts in a study where only a compound of formula I was administered as a single agent to patients with CLL [0157] Figure 56 shows a graphical summary of lymphocyte counts in a study where a compound of formula I in combination with bendamustine (B) or rituximab (R) was administered to patients with CLL.

[0158] Figure 57A shows the contour plots depicting CLL cell viabïlily after 48 hours following treatment with a compound of formula I, bendamustine, fludarabine, dexamethasone, or the drugs combined. Figure 57B shows a bar diagram depicting the mean relative viabilities of CLL cells treated with a compound of formula I (5 μΜ), bendamustine (10 μΜ), fludarabine (10 μΜ), dexamethasone (10 μΜ), or the drugs combined (mean ± SEM, n=9).

[0159] Figure 58A shows a graph quantifying mitochondrial depolarization induced by the

BIM BH3 peptide at 0.03 μΜ final concentration in peripheral blood CLL cells that were BH3 profiled by FACS (n = 30). Figure 58B shows BH3 profiles from three individual patients

showing pattern of prédominant dependence on BCL2, Mcl-1, and Bcl-XL. Figure 58C is a graph showing BIM depolarization in treatment-naïve patients achieving a partial response (PR) or complété response (CR) by 2008 IW-CLL criteria compared to patients with progressive disease (PD) during or within six months of completing frontline CLL therapy (p=0.024).

Figure 58D is a graph showing BIM depolarization in patients with unmutated IGHV status (n=7) compared to patients with mutated IGHV status (n=18) (p=0.0026). Figure 58E is a graph showing the corrélation between percentage of VH homology to germline with level of priming (p=0.0043).

[0160] Figures 59A and 59B show graphs depicting CLL cell adhérence quantified by whole well fluorimetry at 24 hours and 1 hour, respectively (one-tailed p=0.045 and 0.032, respectively). Figure 59C shows a graph depicting CLL cell viability as assessed by Annexin V/PI of PB-derived CLL cells co-cultured in the presence or absence of StromaNKTert for 24 hours, with drug treatments as depicted in the graph. Figure 59D shows dose response curves for CLL cells cultured in the presence of ABT-737 for 24 hours with or without StromaNKTert and with or without compound I, Figure 59E shows dose response curves for CLL cells cultured in the presence of ABT-263 with or without StromaNKTert and with or without compound I. [0161] Figure 60A shows aggregate CLL cell percentage apoptosis as measured by AnnexinV/PI for ail four patient samples. Figure 60B shows a graph depicting mitochondrial depolarization in stroma-exposed CLL cells treated with compound I when compared to controls (one-tailed p=0.0749). Figure 60C shows a graph depicting mitochondrial depolarization in stroma-exposed CLL cells treated with BAD BH3 peptide and ABT-737 with compound I when compared to controls (one-tailed p=0.0462 and 0.0468, respectively).

Modes of Carrying Out the Invention [0162] Uniess otherwise defined, ail terms of art, notations and other scientific terms or terminology used herein are intended to hâve the meanings commonly understood by those of skill in the art to which this invention pertains. In some cases, terms with commonly understood meanings are defined herein for clarity and/or for ready reference, and the inclusion of such définitions herein should not necessarily be construed to represent a substantial différence over what is generally understood in the art. Many of the techniques and procedures described or referenced herein are well understood and commonly employed using conventional methodology by those skilled in the art. As appropriate, procedures involving the use of

commercially available kits and reagents are generally carried out in accordance with manufacturer defined protocols and/or parameters unless otherwise noted.

[0163] The discussion of the general methods given herein is intended for illustrative purposes only. Other alternative methods and embodiments will be apparent to those of skill in the art upon review of this disclosure.

[0164] A group of items linked with the conjunction “or” should not be read as requiring mutual exclusivity among that group, but rather should also be read as “and/or” unless expressly stated otherwise. Although items, éléments, or components of the invention may be described or claimed in the singular, the plural is contemplated to be within the scope thereof unless limitation to the singular is explicîtly stated.

[0165] The invention provides methods that relate to a novel therapeutic strategy for the treatment of cancer and inflammatory diseases. In one aspect, the invention provides a method of treating cancer in a subject comprising administering to said subject a compound of formula A

wherein R is H, halo, or C1-C6 alkyl; R’ is C1-C6 alkyl; or a pharmaceutically acceptable sait thereof; and optionally a pharmaceutically acceptable excipient. [0166] In a particular embodiment, halo is F; and R’ is methyl, ethyl or propyl.

[0167] In a particular embodiment, R is attached to position 5 of the quinazolinyl ring, having the structure

[0168] In a particular embodiment, R is attached to position 6 of the quinazolinyl ring, having the structure

[0169] The term ‘compound’ used herein, unless otherwise specified, refers to a compound of formula A, such as compound I, compound II, or an enantiomer, such as I” or II”, or an enantiomeric mixture.

[0170] The “compound of formula I” or “compound I” refers to the chemical compound

5-fluoro-3-phenyl-2-[l-(9H-purin-6-ylamino)-propyI]-3H-quinazolin-4-one, structure of formula I:

[0171]

The S-enantiomer of compound I is shown here, designated I”:

(I”, S-enantiomer) [0172] The “compound of formula Π” or “compound II” refers to the chemical compound 2(l-(9H-purin-6-ylamino)ethyl)-6-fluoro-3-phenylquinazolin-4(3H)-one, structure of formula II:

[0173]

The S-enantiomer of compound II is shown here, designated II”:

(II”, S-enantiomer) [0174] In one embodiment, the compound of formula A is a compound of formula I. In another embodiment, the compound of formula A is a compound of formula IL In certain embodiments, the compound is a racemic mixture of R- and S-enantiomers. In certain embodiments, the compound is used as a mixture of enantiomers, and is often enriched with the S-enantiomer. In some embodiments, the compound is predominantly the S-enantiomer. In some embodiments, the compound of formula A, used in the methods described herein is at least

80% S-enantiomer. In certain embodiments, the compound is primarily composed of the S-enantiomer, wherein the compound comprises at least 66-95%, or 85-99% of the S-enantiomer. In some embodiments the compound has an enantiomeric excess (e.e.) of at least 90% or at least 95% of S-enantiomer. In some embodiments the compound has an Senantiomeric excess (e.e.) of at least 98% or at least 99%. In certain embodiments, the compound comprises at least 95% of the S-enantiomer. In the cellular and patient experiments provided in the Example section, the sample of compound I used was over 95% S-enantiomer. [0175] In spécifie embodiments, the compound of formula I or II, used in the methods described herein is at least 80% S-enantiomer. In certain embodiments, the compound of formula I or II is primarily composed of the S-enantiomer, wherein the compound comprises at least 66-95%, or 85-99% of the S-enantiomer. In some embodiments the compound of formula I or II has an enantiomeric excess (e.e.) of at least 90% or at least 95% of S-enantiomer. In some embodiments the compound of formula I or II has an S-enantiomeric excess (e.e.) of at least

98% or at least 99%. In certain embodiments, the compound of formula I or II comprises at least 95% of the S-enantiomer. In the cellular and patient experiments provided in the Example section, the sample of compound I used was over 95% S-enantiomer.

[0176] In a particular embodiment, the compound selectively inhibits PI3K. pl 1 Οδ compared to other PI3K isoforms.

[0177] In a particular embodiment, the cancer is a hematological malignancy and/or solid tumor. In another particular embodiment, the hematological malignancy is leukemia or lymphoma.

[0178] In some embodiments, lymphoma is a mature (peripheral) B-cell neoplasm. In spécifie embodiments, the mature B-cell neoplasm is selected from the group consisting of B-cell chronic lymphocytic leukemia ! small lymphocytic lymphoma; B-cell prolymphocytic leukemia; Lymphoplasmacytic lymphoma; Marginal zone lymphoma, such as Splenic marginal zone Bcell lymphoma (+/- villous lymphocytes), Nodal marginal zone lymphoma (+/- monocytoid Bcells), and Extranodal marginal zone B-cell lymphoma of mucosa-associated lymphoid tissue (MALT) type; Hairy cell leukemia; Plasma cell myeloma/plasmacytoma; Follicular lymphoma, follicle center; Mantle cell lymphoma; Diffuse large cell B-cell lymphoma (încluding Médiastinal large B-cell lymphoma, Intravascular large B-cell lymphoma, and Primary effusion lymphoma); and Burkitt's lymphoma/Burkitt's cell leukemia.

[0179] In some embodiments, lymphoma is selected from the group consisting of multiple myeloma (MM) and non-Hodgkin’s lymphoma (NHL), mantle cell lymphoma (MCL), follicular lymphoma, Waldenstrom’s macroglobulinemia (WM) or B-cell lymphoma and diffuse large Bcell lymphoma (DLBCL).

[0180] In a further particular embodiment, leukemia is selected from the group consisting of acute lymphocytic leukemia (ALL), acute myeloid leukemia (AML), chronic lymphocytic leukemia (CLL), and small lymphocytic lymphoma (SLL). Acute lymphocytic leukemia is also known as acute lymphoblastic leukemia and may be used interchangeably herein. Both terms describe a type of cancer that starts from the white blood cells, lymphocytes, in the bone marrow.

[0181] In some embodiments, Non-Hodgkin’s Lymphoma (NHL) falls into one of two categories, aggressive NHL or indolent NHL. Aggressive NHL is fast growing and may lead to a patient’s death relatively quickly. Untreated survival may be measured in months or even weeks. Examples of aggressive NHL includes B-cell neoplasms, diffuse large B-cell lymphoma, T/NK cell neoplasms, anaplastic large cell lymphoma, peripheral T-cell lymphomas, precursor B-lymphoblastîc leukemia/lymphoma, precursor T-lymphoblastic leukemia/lymphoma, Burkitt’s lymphoma, Adult T-cell lymphoma/leukemia (HTLV1+), primary CNS lymphoma, mantle cell lymphoma, polymorphie post-transplantation lymphoproliférative disorder (PTLD),

AIDS-related lymphoma, true histiocytic lymphoma, and blastic NK-cell lymphoma. The most common type of aggressive NHL is diffuse large cell lymphoma.

[0182] Indolent NHL is slow growing and does not display obvious symptoms for most patients until the disease has progressed to an advanced stage. Untreated survival of patients with indolent NHL may be measured in years. Non-limiting examples include follicular lymphoma, small lymphocytic lymphoma, marginal zone lymphoma (such as extranodal marginal zone lymphoma (also called mucosa associated lymphoid tissue - MALT lymphoma), nodal marginal zone B-cell lymphoma (monocytoid B-cell lymphoma), splenic marginal zone lymphoma), and lymphoplasmacytic lymphoma (Waldenstrom’s macroglobulinemia).

[0183] In some cases, histologie transformation may occur, e.g., indolent NHL in patients may convert to aggressive NHL.

[0184] In some embodiments, the invention provides methods of treating a patient with aggressive NHL or indolent NHL.

[0185] In some embodiments, the invention provides methods of treating a patient with a condition selected from the group consisting of mantle cell lymphoma (MCL), diffuse large B cell lymphoma (DLBCL), follicular lymphoma (FL), acute lymphocytic leukemia (ALL), acute myeloid leukemia (AML), chronic lymphocytic leukemia (CLL), and small lymphocytic lymphoma (SLL), multiple myeloma (MM), and marginal zone lymphoma.

[0186] In some embodiments, the methods of the invention are administered to patients with relapsed or refractory conditions.

[0187] In another embodiment, the cancer is breast, lung, colon or prostate cancer.

[0188] In a particular embodiment, the cancer is associated with abnormal PI3K activity compared to PI3K activity in a subject without cancer.

[0189] In a particular embodiment, the preferred subject is refractory to chemotherapy treatment, or in relapse after treatment with chemotherapy. In an alternative embodiment, the subject is a de novo patient.

[0190] In a particular embodiment, the method comprises reducing the level of PI3KÔ activity in said patient.

[0191] In a particular embodiment, the subject is a human subject.

[0192] Subjects that undergo treatment with known therapeutic agents may expérience résistance to treatment. For example, although bortezomib was FDA approved for relapsed/refractory, relapsed, and newly diagnosed MM, some patients do not respond and

others acquire résistance to bortezomib. In some embodiments, the quinazolinone compound described herein synergistically augments efficacy of a known therapeutic agent. In some embodiments, the compounds described herein can augment any of the therapeutic agents described herein. In more spécifie embodiments, the compounds described herein synergistically augment protéasome inhibitors. In some of the foregoing embodiments, the subject is résistant to chemotherapeutic treatment. In some of the foregoing embodiments, the subject is résistant to protéasome inhibitors. In some of the foregoing embodiments, the subject is résistant bortezomib or carfilzomib. In one example, the compounds described herein synergistically augment bortezomib-induced MM cytotoxicity. Without being bound by theory, in some embodiments, the compounds discussed herein inhibit bortezomib-induced phosphorylation of AKT. In some embodiments, the methods described herein are used to overcome résistance to protéasome inhibitor treatment. In some embodiments, the invention provides a method to treat a subject that is résistant or has developed a résistance to therapeutic agents.

[0193] While not being bound by theory, the synergistic effects between a compound of formula A and conventional thérapies may be attributed to the ability of the compound of the invention to induce tumor cell mobilization into peripheral circulation. Inducing the peripheral circulation of the tumor cells increases the ability of conventional therapy to act upon and more effectively neutralize the tumor. This synergy has been demonstrated in CLL patients.

[0194] Accordingly, the method comprises administering in addition to a compound of formula A to a patient, a therapeutically effective amount of at least one additional therapeutic agent and/or a therapeutic procedure selected to treat said cancer in said patient. “Therapeutic agent” may refer to one or more compounds, as used herein. The therapeutic agent may be a standard or experimental chemotherapy drug. The therapeutic agent may comprise a combination of more than one chemotherapy drug. Typical chemotherapy drug combinations are listed a-q herein. A particular therapeutic agent may be chosen depending on the type of disease being treated. Non-limiting examples of conventional chemotherapeutic treatments for particular hématologie disease are described in later sections. In a particular embodiment, the invention provides a method to treat a hematopoietic cancer patient, e.g., a CLL patient, with bortezomib and a compound of formula A (e.g., formula I, II, I”, or II”), wherein the combination provides a synergistic effect.

[0195] In a particular embodiment, said therapeutic agent is selected from the following group consisting of bortezomib (Velcade®), carfilzomib (PR-171), PR-047, disulfiram, lactacystin, PS519, eponemycin, epoxomycin, aclacinomycin, CEP-1612, MG-132, CVT-63417, PS-341, vinyl sulfone tripeptide inhibitors, ritonavir, PI-083, (+/-)-7-methylomuralide, (-)-7-methylomuralide, perifosine, rituximab, sildenafil citrate (Viagra®), CC-5103, thalidomide, epratuzumab (hLL2anti-CD22 humanized antibody), simvastatin, enzastaurin, Campath-1H®, dexamethasone, DT PACE, oblimersen, antineoplaston A10, antineoplaston AS2-1, alemtuzumab, beta alethine, cyclophosphamide, doxorubicin hydrochloride, PEGylated liposomal doxorubicin hydrochloride, prednisone, prednisolone, cladribine, vincristine sulfate, fludarabine, filgrastim, melphalan, recombinant interferon alfa, carmustine, cisplatin, cyclophosphamide, cytarabine, etoposide, melphalan, dolastatin 10, indium In 111 monoclonal antibody MN-14, yttrium Y 90 humanized epratuzumab, anti-thymocyte globulin, busulfan, cyclosporine, methotrexate, mycophenolate mofetil, therapeutic allogeneic lymphocytes, Yttrium Y 90 ibritumomab tiuxetan, sirolimus, tacrolimus, carboplatin, thiotepa, paclitaxel, aldesleukin, recombinant interferon alfa, docetaxel, ifosfamide, mesna, recombinant interleukin-12, recombinant interleukin-11, Bcl-2 family protein inhibitor ABT-263, denileukin diftitox, tanespimycin, everolimus, pegfilgrastim, vorinostat, alvocidib, recombinant flt3 ligand, recombinant human thrombopoietin, lymphokine-activated killer cells, amifostine trihydrate, aminocamptothecin, irinotecan hydrochloride, caspofungin acetate, clofarabine, epoetin alfa, nelarabîne, pentostatin, sargramostim, vinorelbine dîtartrate, WT-1 analog peptide vaccine, WT1 126-134 peptide vaccine, fenretinide, ixabepilone, oxaliplatin, monoclonal antibody CD19, monoclonal antibody CD20, omega-3 fatty acids, mitoxantrone hydrochloride, octreotide acetate, tositumomab and iodine 1131 tositumomab, motexafin gadolinium, arsenic trioxide, tipifarnib, autologous human tumor-derived HSPPC-96, veltuzumab, bryostatin 1, anti-CD20 monoclonal antibodies, chlorambucil, pentostatin, lumiliximab, apolizumab, Anti-CD40, and ofatumumab, or a combination thereof. Combination of therapeutic agents are used in current and experimental thérapies such as those combinations a-q listed above.

[0196] In some embodiments, the therapeutic agent is preferably a protéasome inhibitor. In some embodiments, the methods comprise administering a compound with a protéasome inhibitor. Protéasome inhibitors include natural and synthetic compounds. Non-limiting examples of protéasome inhibitors include bortezomib, ([(lR)-3-methyl-l-({(2S)-3-phenyl-2[(pyrazîn-2-ylcarbonyl)amino]propanoyl}amino)butyl]boronic acid), which is marketed as

Velcade®' by Millennium pharmaceuticals; carfilzomib (PR-171) and the oral analog, PR-047, both of which are developed by Proteolix, Inc. Other examples of protéasome inhibitors include disulfiram; lactacystin; synthetic compounds such as PS-519, eponemycin, epoxomycin, and aclacinomycin; calpain inhibitors, such as CEP-1612, MG-132, CVT-63417, PS-341; vinyl sulfone tripeptide inhibitors; ritonavir; PI-083; (+/-)-7-methylomuralide; and (-)-7methylomuralide. In particular embodiments, the compound of formula A is administered in combination with bortezomib or carfilzomib. In more particular embodiments, the compound of formula I is administered in combination with bortezomib or carfilzomib. In other particular embodiments, the compound of formula II is administered in combination with bortezomib or carfilzomib. In particular embodiments, the compound of formula A is administered in combination with rituximab or ofatumumab. In more particular embodiments, the compound of formula I is administered in combination with rituximab or ofatumumab. In other particular embodiments, the compound of formula II is administered in combination with rituximab or ofatumumab.

[0197] In some embodiments, the therapeutic agent is an alkylating agent. Non-limiting examples of alkylating agents include for instance Busulfan, melphalan, chlorambucil, clyclophosphamide, mechlorethamine, uramustine, ifosfamide, carmustine, lomustine, streptozocin, thiotepa, and platinum based chemotherapeutic drugs, such acisplatin, carboplatin, nedaplatin, oxaliplatin, satraplatin, tripla tin tetranitrate.

[0198] In other embodiments, any compound of the invention may be combined with one or more other active therapeutic agents in a unitary dosage form for simultaneous or sequential administration to a patient. The combination therapy may be administered as a simultaneous or sequential regimen. When administered sequentially, the combination may be administered in two or more administrations.

[0199] In one embodiment, co-administration of a compound of the invention with one or more other active therapeutic agents generally refers to simultaneous or sequential administration of a compound of the invention and one or more other active therapeutic agents, such that therapeutically effective amounts of the compound of the invention and one or more other active therapeutic agents are both présent in the body of the patient. In an alternative embodiment, the compound and therapeutic agent(s) are not necessarily both présent in the body of the patient but the particular dosing schedule the compound and therapeutic agents results in synergistic effects.

[0200] Co-administration includes administration of unit dosages of the compounds of the invention before or after administration of unit dosages of one or more other active therapeutic agents; for example, administration of the compounds of the invention within seconds, minutes, hours or days of the administration of one or more other active therapeutic agents. For example, a unit dose of a compound of the invention can be administered first, followed within seconds, minutes, hour or days by administration of a unit dose of one or more other active therapeutic agents. Alternatively, a unit dose of one or more other therapeutic agents can be administered first, followed by administration of a unit dose of a compound of the invention within seconds, minutes, hours or days. In some cases, it may be désirable to administer a unit dose of a compound of the invention first, followed, after a period of hours (e.g., 1-12 hours), by administration of a unit dose of one or more other active therapeutic agents. In other cases, it may be désirable to administer a unit dose of one or more other active therapeutic agents first, followed, after a period of hours (e.g., 1-12 hours), by administration of a unit dose of a compound of the invention. In some cases, it may be désirable to administer a unit dose of a compound of the invention first, followed, after a period of days (e.g., 1-12 days), by administration of a unit dose of one or more other active therapeutic agents. In other cases, it may be désirable to administer a unit dose of one or more other active therapeutic agents first, followed, after a period of days (e.g., 1-12 days), by administration of a unit dose of a compound of the invention. In some embodiments the dosing regimen may involve alternating administration of compound and therapeutic agent over a period of several days, weeks, or months.

[0201] The combination therapy may provide “synergy” and “synergistic effect”, i.e. the effect achieved when the active ingrédients used together is greater than the sum of the effects that results from using the compounds separately. A synergistic effect may be attained when the active ingrédients are: (1) co-formulated and administered or delivered simultaneously in a combined formulation; (2) delivered by alternation or in parallel as separate formulations; or (3) by some other regimen. When delivered in alternation therapy, a synergistic effect may be attained when the compounds are administered or delivered sequentially, e.g., in separate tablets, pills or capsules, or by different injections in separate syringes. In general, during alternation therapy, an effective dosage of each active ingrédient is administered sequentially, i.e. serially. [0202] In one aspect, the invention provides a pharmaceutical composition comprising a compound of Formula I:

or a pharmaceutically acceptable sait thereof; and at least one pharmaceutically acceptable excipient. In one embodiment, the composition is enriched with the S-enantiomer. [0203] In another aspect, the invention provides a pharmaceutical composition comprising a compound of Formula II:

or a pharmaceutically acceptable sait thereof; and at least one pharmaceutically acceptable excipient. In one embodiment, the composition is enriched with the S-enantiomer. [0204] In one aspect, the invention provides a method of treating multiple myeloma (MM) in a patient comprising administering a combination of a compound of formula A and an additional therapeutic agent. In some embodiments, formula A is compound I or II. In spécifie embodiments, formula A is compound I”. In other embodiments, formula A is compound II”. In some of the foregoing embodiments the additional therapeutic agent is a protéasome inhibitor. In spécifie embodiments the additional therapeutic agent is bortezomib. In a spécifie embodiment, the method of treating multiple myeloma in a patient comprises administering compound I” with bortezomib. In a spécifie embodiment, the method of treating multiple myeloma in a patient comprises administering compound II” with bortezomib. In some of the foregoing embodiments, compound I” or II” has an enantiomeric excess of at least 60%. In some of the foregoing embodiments, compound I” or II” has an enantiomeric excess of at least 70%. In some of the foregoing embodiments, compound I” or II” has an enantiomeric excess of at least 80%. In some of the foregoing embodiments, compound I” or II” has an enantiomeric excess of at least 90%. In some of the foregoing embodiments, compound I” or II” has an

enantiomeric excess of at least 95%. In some of the foregoing embodiments, compound I” or II” has an enantiomeric excess of at least 98%. In some of the foregoing embodiments, compound

I” or II” has an enantiomeric excess of at least 99%.

[0205] In a particular embodiment, a combination of therapeutic agents is administered with a compound of Formula A, wherein said combination is selected from the group consisting of

a) bendamustine;

b) rituximab;

c) bendamustine and rituximab;

d) ofatumumab; and

e) lenalidomide.

[0206] In alternative embodiments, the compound is used in combination with a therapeutic procedure. In a particular embodiment, the therapeutic procedure is selected from the group consisting of peripherai blood stem cell transplantation, autologous hematopoietic stem cell transplantation, autologous bone marrow transplantation, antibody therapy, biological therapy, enzyme inhibitor therapy, total body irradiation, infusion of stem cells, bone marrow ablation with stem cell support, in vziro-treated peripherai blood stem cell transplantation, umbilical cord blood transplantation, immunoenzyme technique, immunohistochemistry staining method, pharmacological study, low-LET cobalt-60 gamma ray therapy, bleomycin, conventional surgery, radiation therapy, high-dose chemotherapy and nonmyeloablative allogeneic hematopoietic stem cell transplantation.

[0207] In a particular embodiment, the method further comprises obtaining a biological sample from said patient; and analyzing said biological sample with an analytical procedure selected from the group consisting of blood chemistry analysis, chromosomal translocation analysis, needle biopsy, fluorescence in situ hybridization, laboratory biomarker analysis, immunohistochemistry staining method, flow cytometry or a combination thereof.

[0208] For nomenclature purposes, the quinazolinyl and purinyl components of the compound are numbered accordingly:

8	1
7	Ά2
6
quinazoline 5	4

[0209] As used herein, the term “alkyl,” includes straight-chain, branched-chain and cyclic monovalent hydrocarbyl radicals, and combinations of these, which contain only C and H when they are unsubstituted. Examples include methyl, ethyl, isobutyl, cyclohexyl, cyclopentylethyl, and the like. The total number of carbon atoms in each such group is sometimes described herein, e.g,, when the group can contain up to ten carbon atoms it can be represented as 1-10C or as C1-C10 or Cl-10.

[0210] “Halo”, as used herein, includes fluoro, chloro, bromo and iodo. Fluoro and chloro are often preferred.

[0211] The term “sélective PI3KÔ inhibitor” or “sélective ΡΙ3Κβ inhibitor”, etc., as used herein, refers to a compound that inhibits the ΡΙ3Κδ or ΡΙ3Κβ isozyme, respectively, more effectively than at least one other isozymes of the PI3K family. The sélective inhibitor may also be active against other isozymes of PI3K, but requires higher concentrations to achieve the same degree of inhibition of the other isozymes. “Sélective” can also be used to describe a compound that inhibits a particular PÏ3-kinase more so than a comparable compound. A “sélective PI3KÔ inhibitor” compound is understood to be more sélective for PI3KÔ than compounds conventionally and generically designated PI3K inhibitors, e.g., wortmannin or LY294002. Concomitantly, wortmannin and LY294002 are deemed “nonselective PI3K inhibitors.” In certain embodiments, compounds of any type that selectively negatively regulate PI3K.Ô expression or activity can be used as sélective ΡΙ3Κδ inhibitors in the methods ofthe invention.

Moreover, compounds of any type that selectively negatively regulate ΡΙ3Κδ expression or activity and that possess acceptable pharmacological properties can be used as sélective PI3KÔ inhibitors in the therapeutic methods of the invention. Without being bound by theory, targeting pllO delta inhibition with a compound of the invention provides a novel approach for the treatment of hematological malignancies because this method inhibits constitutive signaling resulting in direct destruction of the tumor cell. In addition, without being bound by theory, pllO delta inhibition represses microenvironmental signais which are crucial for tumor cell homing, survival and prolifération.

[0212] In an alternative embodiment, compounds of any type that selectively negatively regulate ΡΙ3Κβ expression or activity can be used as sélective ΡΙ3Κβ inhibitors in the methods of the invention. Moreover, compounds of any type that selectively negatively regulate ΡΙ3Κβ expression or activity and that possess acceptable pharmacological properties can be used as sélective ΡΙ3Κβ inhibitors in the therapeutic methods of the invention.

[0213] “Treating” as used herein refers to inhibiting a disorder, i.e., arresting its development; relieving the disorder, i.e., causing its régression; or ameliorating the disorder, i.e., reducing the severity of at least one of the symptoms associated with the disorder. In some embodiments, “treating” refers to preventing a disorder from occurring in an animal that can be predisposed to 5 the disorder, but has not yet been diagnosed as having it. “Disorder” is intended to encompass medical disorders, diseases, conditions, syndromes, and the like, without limitation.

[0214] In another aspect, the invention includes a method for suppressing a function of basophils and/or mast cells, and thereby enabling treatment of diseases or disorders characterized by excessive or undesirable basophil and/or mast cell activity. According to the 10 method, a compound of the invention can be used that selectively inhibits the expression or activity of phosphatidylinositol 3-kinase delta (PI3KÔ) in the basophils and/or mast cells. Preferably, the method employs a PI3KÔ inhibitor in an amount sufficient to inhibit stimulated histamine release by the basophils and/or mast cells. Accordingly, the use of such compounds and other ΡΙ3Κδ sélective inhibitors can be of value in treating diseases characterized by histamine release, i.e., allergie disorders, including disorders such as chronic obstructive pulmonary disease (COPD), asthma, ARDS, emphysema, and related disorders.

[0215] The method can hâve utility in treating subjects who are or can be subject to reperfusion injury, i.e., injury resulting from situations in which a tissue or organ expériences a period of ischemia followed by reperfusion. The term “ischemia” refers to localized tissue anémia due to obstruction of the inflow of arterial blood. Transient ischemia followed by reperfusion characteristically results in neutrophil activation and transmigration through the endothélium of the blood vessels in the affected area. Accumulation of activated neutrophils in turn results in génération of reactive oxygen métabolites, which damage components of the involved tissue or organ. This phenomenon of “reperfusion injury” is commonly associated with conditions such as vascular stroke (including global and focal ischemia), hémorrhagie shock, myocardial ischemia or infarction, organ transplantation, and cérébral vasospasm. To illustrate, reperfusion injury occurs at the termination of cardiac bypass procedures or during cardiac arrest when the heart, once prevented from receiving blood, begins to reperfuse. It is expected that inhibition of PI3KÔ activity will resuit in reduced amounts of reperfusion injury in 30 such situations.

[0216] In certain embodiments, the invention provides methods to treat a solid tumor. In spécifie embodiments, the cancer is breast, lung, colon, or prostate cancer. In certain

embodiments, the invention provides methods to treat a solid tumor that is associated with abnormal or undesirable cellular signaling activity mediated by ΡΙ3Κβ. In certain embodiments, a solid tumor is selected from the group consisting of pancreatic cancer; bladder cancer; colorectal cancer; breast cancer, including metastatic breast cancer; prostate cancer, including androgen-dependent and androgen-independent prostate cancer; rénal cancer, including, e.g., metastatic rénal cell carcinoma; hepatocellular cancer; lung cancer, including, e.g., non-small cell lung cancer (NSCLC), bronchioloalveolar carcinoma (BAC), and adenocarcinoma of the lung; ovarian cancer, including, e.g., progressive épithélial or primary peritoneal cancer; cervical cancer; gastric cancer; esophageal cancer; head and neck cancer, including, e.g., squamous cell carcinoma of the head and neck; melanoma; neuroendocrine cancer, including metastatic neuroendocrine tumors; brain tumors, including, e.g., glioma, anaplastic oligodendroglioma, adult glioblastoma multiforme, and adult anaplastic astrocytoma; bone cancer; and soft tissue sarcoma.

[0217] Genetic ablation of pl 10δ has been found to resuit in mild phenotype restricted to immune systern. General observations include organisms that are fertile with no gross anatomical or behavioral abnormalities. A histological examination revealed major organs to appear normal. The total class IPI3K activity was reduced 30-50% in B and T cells. In addition, no increase in susceptibility to infections was observed. Furthermore, the effect on the hematopoietic System includes normal peripheral blood cell counts, the occurrence of lymphoid hypoplasia and the lack of germinal centers in spleen and lymph nodes, a reduced number of B220 + IgM + B cell progenitors in bone marrow, a reduced level of sérum immunoglobulin, and normal T cell development in the thymus.

[0218] Genetic ablation of pl 10δ affects myeloid and B cell signaling, which is important for oncogenesis. In particular, tyrosine kinase signaling, development, prolifération and survival are affected in myeloid cells. B cell function is most affected and includes prolifération, différentiation, apoptosis, and response to B cell survival factors (BCR, CD40, IL-4, chemokines). Thus, the invention includes methods of treating disease states in which one or more of these myeloid and B cell functions are abnormal or undesirable.

[0219] A pan PI3K inhibitor that targets on a molecular level pl 10a, pl 10β, pl 10δ, pl 10γ, (hvPS34, mTOR, DNA-PK, and others), in turn targets ail tissues. The potential clinical indication includes cancer but clinical adverse events include hyperinsulinemia in cancer patients. The advantage of a pl 108 sélective inhibitor which targets cells mediating

inflammation and cancer cells, wherein potential clinical indication include cancer, rheumatoid arthritis, asthma, allergies and COPD, is that treatment is well tolerated, and side effects like hyperinsulinemia are avoided. Thus in one aspect the invention provides a method to treat patients having insulin résistance, or type 2 diabètes, for cancer, rheumatoid arthritis, asthma, allergies, COPD, or other conditions treatable with the compounds of the invention. For patients needing such treatment who hâve excessive insulin conditions or tendencies, the compounds of the invention are partîcularly advantageous over pan-PI3K inhibitors. In certain embodiments, a compound of formula I or I” is preferred because it provides therapeutic benefits to treating hématologie malignancies without adversely affecting insulin signaling.

[0220] In one embodiment, the invention relates to methods of inhibiting PI3K pl 10δ. In another embodiment, the invention relates to methods of inhibiting PI3K pl ΙΟβ or pl 10γ. [0221] In certain embodiments, the method described herein has little or no off target activity. In particular, compound of formula I used in the method show little activity against over 300 protein kinases including those summarized in Table 3 of Example 16. In certain embodiments, the method described herein has no or minimal hyperinsulinemia effects in cancer patients compared to methods comprising the administration of pan-PI3K inhibitors. In certain embodiments, the method described herein is useful in targeting cells mediating Akt phosphorylation, because the compounds of Formula A inhibît Akt phosphorylation. Suitable patients for treatment with the compounds of the invention can thus be selected, in one embodiment, by selecting a patient exhibiting elevated Akt phosphorylation associated with a hematopoietic cancer such as lymphoma, leukemia or multiple myeloma.

[0222] The methods herein avoid off-target liabilities and are characterized by négative results in receptor gram screens, having no hERG inhibition and no significant P450 inhibition.

[0223] Another advantage of the inventive method is the absence of adverse cardiovascular, respiratory, or central nervous system effects as demonstrated in safety pharmacology studies. In addition, a 28-day toxicity study in rats and dogs demonstrated a high therapeutic index, e.g., a NOAEL (no observable adverse effect level) » 10 μΜ. This is the highest experimental dose of a chemical at which there is no statistically or biologicaily significant increase in frequency or severity of a toxicological effect between an exposed group and its appropriate control. Adverse effects are defined as any effects that resuit in functional impairment and/or pathological lésions that may affect the performance of the whole organism or that reduce an organism’s ability to respond to an additional challenge.

[0224] In certain embodiments, the method described herein markedly reduces lymphadenopathy, forcing lymphocytes into the circulation. This lymphocyte redistribution has the potential benefit of placing the malignant CLL cells into a less protected environment outside of the lymph nodes. In certain embodiments, the coadministration of chemotherapy and/or immunotherapy with an agent like a compound of formula A enhances the killing of CLL cells while simultaneously reducing lymphocytosis.

[0225] In another embodiment, the inventive methods are non-genotoxic in a standard battery of tests.

[0226] Another advantage of the invention is that compound selectivity for one or two PI3K 10 îsoforms results in an improved safety profile over compounds having pan-PI3K inhibition. In yet another advantage, compound I has a favorable pharmacokinetic profile with good target coverage, and no adverse effects on glucose or insulin levels, and is well tolerated at doses above commonly used therapeutic doses by normal healthy volunteers. Another advantage of the invention includes the ability to treat a wide range of hematological malignancies as demonstrated by the examples herein.

[0227] In certain embodiments, the methods of the invention are directed towards treating a cancer. In certain embodiments, the cancer is a hematological malignancy. In spécifie embodiments, the hematological malignancy is selected from the group consisting of acute lymphocytic leukemia (ALL), acute myeloid leukemia (AML), chronic lymphocytic leukemia 20 (CLL), multiple myeloma (MM), and non-Hodgkin lymphoma (NHL). In certain embodiments, the non-Hodgkin lymphoma is selected from the group consisting of large diffuse B-cell lymphoma (LDBCL), mantle cell lymphoma (MCL), Waldenstrom’s macroglobulinemia (WM) and lymphoplasmacytic lymphoma.

[0228] PI3K is implicated in many hematological malignancies and preclinical proof of concept relating to treatment with compound I has been established. The table below summarizes particular hematological malignancies and the method of action on the primary patient cell or disease cell line.

Indication

Chronic Lymphocytic Leukemia (CLL)

Acute Myelogenous Leukemia (AML)

Acute Lymphocytic Leukemia (ALL)

Non-Hodgkin’s Lymphomas (NHL) (MCL, DLBCL, FL)

Multiple Myeloma (MM)

Effects of compounds of formula A

Primary patient cells Induces apoptosis Blocks survival factors

Primary patient cells Blocks PI3K signaling Inhibits prolifération

Cell Lines

Blocks PI3K signaling Induces apoptosis

Cell Lines

Blocks PI3K signaling Induces apoptosis

Primary patient cells P110 δ overexpressed in 24/24 samples Induces apoptosis [0229] Data provided herein demonstrates that the compounds of the invention are useful to treat lymphomas and leukemias. Lymphomas and leukemias generally express the delta isoform of pllO selectively, e.g., Figure 15 demonstrates that pl 103 is prévalent in most lymphoma cell lines, while pl 10a is not generally observed. Moreover, data presented in Figure 16A shows that cell cultures from six different leukemia cell lines were sensitive to Compound I, and were strongly affected by 5-10 micromolar concentrations of this compound. Figures 8 and 9 support compound I as reducing Akt(Ser473) production in several cell lines,, [0230] CLL, for example, produces mainly pl 10δ and to a lesser extent pl 10γ for signaling purposes, thus compounds that inhibit p 110δ and/or p 110γ are expected to exhibit sélective cytotoxicity towards these cells. Example 3 shows dose-dependent cytotoxicity for compound I (Figure 3), in CLL cells, including cells taken from poor prognosis patients (Figure 19), and cells from patients shown to be résistant to other CLL treatments (Figure 20). In addition, Example 13 and Figure 13 demonstrate that compound I administered to a CLL patient at a rate of 50 mg BID for a 28-day cycle provides a significant therapeutic effect. An ALC concentration percent decrease in lymphocytes is observed. Thus in one aspect, the invention provides methods for treating CLL patients with drug-resistant CLL using compounds of Formula A. On the other hand, Example 17 suggests that a fibroblast cell line relying mainly on

pl ΙΟα for signaling was not sensitive to Compound I. Thus in one aspect, patient sélection can include excluding patients having a cancer that relies mainly on pl ΙΟα for signaling.

[0231] The compounds of Formula A are also useful to treat lymphoma, including both B-cell and T-cell lymphomas. Data in Figure 4 demonstrates that six different ALL cell lines were sensitive to Compound I, which caused a significant réduction in cell viability in ail six cell lines. Figure 12 and Example 12 demonstrate that mantle cell lymphoma patients treated with 50 mg BID of Compound I for 28 days experienced on average a 44% decrease in tumor burden. Moreover, Figure 14 demonstrates that an MCL patient at the end of the 28 day cycle experienced similar plasma levels of Compound I following administration of a 50 mg dose to that observed in a normal healthy volunteer (NHV); thus the compound does not build up excessively over the course of a cycle of treatment, nor does the patient become tolérant by increased metabolism over the course of a treatment cycle.

[0232] In addition, the compounds of Formula A, or Formula I, are useful to treat hematopoietic cancers that constitutively express Akt phosphorylation activity. Example 8, and

Figures 8 and 9 list cancer cell lines that demonstrate constitutive Akt phosphorylation, including B-cell lymphomas, T-cell lymphomas, ALL, malignant histiocytosis, DLBCL and AML. Exposure of the cell to compound I results in the réduction of Akt phosphorylation. See also Example 19, which shows that constitutive Akt phosphorylation was inhibited by Compound I in 13 of 13 cell lines.

[0233] In certain embodiments, the cancer is a solid tumor. In spécifie embodiments, the cancer is breast, ovarian, lung, colon, or prostate cancer. Figure 6, for example, shows that Compound I reduces cellular prolifération of two breast cancer cell lines, and Figure 10 illustrâtes cytotoxicîty to three different breast cancer cell lines. Similarly, Figure 7 demonstrates that Compound I is cytotoxic to two ovarian cancer cell lines.

[0234] For the treatment of a solid tumor, it is advantageous to use a compound of Formula A that expresses good activity (e.g., IC50 less than about 1 μΜ, and preferably less than about 250 nM—see Example 15) against pl 10β, since solid tumors often utilize this îsozyme rather than or more than pl 10Ô. Thus a compound of formula A that has an IC50 less than about 250 nM is preferred for treatment of a solid tumor; compound I, I”, II, or II” is suitable for this use, as demonstrated herein.

[0235] In some embodiments, the subject for treatments described herein as one who has been diagnosed with at least one of the conditions described herein as treatable by the use of a

compound of Formula A. In some embodiments, the subject has been diagnosed with a cancer named herein, and has proven refractory to treatment with at least one conventional chemotherapeutic agent. For instance, patients who have failed to respond to treatments such as protéasome inhibitors, autologous stem cell transplant, CHOP regimens, rituximab, fludarabine, 5 alemtuzumab, conventional anticancer nucleoside analogues and alkylating agents frequently respond to the methods of treatment described herein. Thus, in one embodiment, the treatments of the invention are directed to patients who have received one or more than one such treatment. [0236] In certain embodiments, the methods of the invention are directed to B-cell, or B lymphocyte, related diseases. B-cells play a rôle in the pathogenesis of autoimmune diseases.

[0237] The compounds of Formula A (particularly Formulas I, I”, Il and II”) are suitable for treating a variety of subjects having the conditions described herein, especially hematological cancers in humans. In some embodiments, the subject selected for treatment of a hematological malignancy that is a subject experiencing relapse after other treatments or is refractory to other treatments. In some embodiments, the subject is selected for treatment of a hematological malignancy that is résistant to other cancer drugs. In some embodiments, the subject is selected for treatment of a hematological malignancy that exhibits a high level of pl 1 Οδ activity. In some embodiments, the subject îs selected for treatment of a hematological malignancy that exhibits a relatively low level of pl 10a activity. In some embodiments, the subject is selected for treatment of a hematological malignancy that constitutive!y expresses Akt phosphorylation activity.

[0238] In one embodiment, the method described herein comprises administering to a subject a compound of formula A described herein, in combination with a therapy used to treat cancer. “Therapy” or “treatment”, as used herein, is a treatment of cancer by any well-known conventional or experimental form of treatment used to treat cancer that does not include the use 25 of a compound of formula A. In certain embodiments, the combination of a compound of formula A with a conventional or experimental therapy used to treat cancer or an autoimmune disease provides bénéficiai and/or désirable treatment results superior to results obtained by treatment without the combination. In certain embodiments, thérapies used to treat cancer are well-known to a person having ordinary skill in the art and are described in the literature.

Thérapies include, but are not limited to, chemotherapy, combinations of chemotherapy, biological thérapies, immunotherapy, radioimmunotherapy, and the use of monoclonal antibodies, and vaccines.

vZ

[0239] In some of the foregoing embodiments, the combination method provides for a compound of formula A administered simultaneously with or during the period of administration of the therapy. In some of the foregoing embodiments the compound of formula A is administered simultaneously with the other chemotherapeutic treatment. In certain embodiments, the combination method provides for a compound of formula A administered prior to or after the administration of the therapy.

[0240] In some of the foregoing embodiments, the subject is refractory to at least one standard or experimental chemotherapy. In some of the foregoing embodiments, the subject is refractory to at least two standard or experimental chemotherapies. In some of the foregoing embodiments, 10 the subject is refractory to at least three standard or experimental chemotherapies. In some of the foregoing embodiments, the subject is refractory to at least four standard or experimental chemotherapies.

[0241] In some of the foregoing embodiments, the subject is refractory to at least one standard or experimental chemotherapy selected from the group consisting of fludarabine, rituximab, alkylating agents, alemtuzumab and the chemotherapy treatments a-q listed above.

[0242] In some of the foregoing embodiments, the subject is refractory to at least two standard or experimental chemotherapies selected from the group consisting of fludarabine, rituximab, alkylating agents, alemtuzumab and the chemotherapy treatments a-q listed above.

[0243] In some of the foregoing embodiments, the subject is refractory to at least three standard or experimental chemotherapies selected from the group consisting of fludarabine, rituximab, alkylating agents, alemtuzumab and the chemotherapy treatments a-q listed above.

[0244] In some of the foregoing embodiments, the subject is refractory to at least four standard or experimental chemotherapies selected from the group consisting of fludarabine, rituximab, alkylating agents, alemtuzumab and the chemotherapy treatments a-q listed above.

[0245] The exact details regarding the administration of the combination may be determined experimentally. The refinement of sequence and timing of administering a compound of formula

A with a selected therapy will be tailored to the individual subject, the nature of the condition to be treated in the subject, and generally, the judgment of the attending practitioner.

[0246] Non-limiting examples of experimental or standard therapy are described below. In addition, treatment of certain lymphomas is reviewed in Cheson, B.D., Leonard, J,P,, “Monoclonal Antibody Therapy for B-Cell Non-Hodgkin’s Lymphoma” The New England

Journal of Medicine 2008, 359(6), p. 613-626; and Wierda, W.G., “Current and Investigational

Thérapies for Patients with CLL” Hematology 2006, p. 285-294. Lymphoma incidence patterns in the United States is profiled in Morton, L.M., et al. “Lymphoma Incidence Patterns b y WHO Subtype in the United States, 1992-2001” Blood 2006,107(1), p. 265-276.

[0247] Treatment of non-Hodgkin’s lymphomas, especially of B cell origin, include, but are not limited to use of monoclonal antibodies, standard chemotherapy approaches (e.g., CHOP, CVP, FCM, MCP, and the like), radioimmunotherapy, and combinations thereof, especially intégration of an antibody therapy with chemotherapy.

[0248] Non-limiting examples of unconjugated monoclonal antibodies for Non-Hodgkin’s lymphoma/B-cell cancers include rituximab, alemtuzumab, human or humanized anti-CD20 antibodies, lumiliximab, anti-TRAIL, bevacizumab, galiximab, epratuzumab, SGN-40, and antiCD74. Non-limiting examples of experimental antibody agents used in treatment of NonHodgkin’s lymphoma/B-cell cancers include ofatumumab, ha20, PRO131921, alemtuzumab, galiximab, SGN-40, CHIR-12.12, epratuzumab, lumiliximab, apolizumab, milatuzumab, and bevacizumab. Any of the monoclonal antibodies can be combined with rituximab, fludarabine, or a chemotherapy agent/regimen.

[0249] Non-limiting examples of standard regimens of chemotherapy for Non-Hodgkin’s lymphoma/B-cell cancers include CHOP (cyclophosphamide, doxorubicin, vincristine, prednisone), FCM (fludarabine, cyclophosphamide, mitoxantrone), CVP (cyclophosphamide, vincristine and prednisone), MCP (mitoxantrone, chlorambucil, and prednisolone), R-CHOP (rituximab plus CHOP), R-FCM (rituximab plus FCM), R-CVP (rituximab plus CVP), and R-MCP (R-MCP).

[0250] Non-limiting examples of radioimmunotherapy for Non-Hodgkin’s lymphoma/B-cell cancers include yttrium-90-labeled ibritumomab tiuxetan, and iodine-131-labeled tositumomab. These therapeutic agents are approved for use in subjects with relapsed or refractory follicular or low-grade lymphoma.

[0251] Therapeutic treatments for mantle cell lymphoma include combination chemotherapies such as CHOP (cyclophosphamide, doxorubicin, vincristine, prednisone), hyperCVAD (hyperfractionated cyclophosphamide, vincristine, doxorubicin, dexamethasone, methotrexate, cytarabine) and FCM (fludarabine, cyclophosphamide, mitoxantrone). In addition, these regimens can be supplemented with the monoclonal antibody rituximab (Rituxan) to form combination thérapies R-CHOP, hyperCVAD-R, and R-FCM. Other approaches include

combining any of the abovementioned thérapies with stem cell transplantation or treatment with ICE (iphosphamide, carboplatin and etoposide).

[0252] Another approach to treating mande cell lymphoma includes immunotherapy such as using monoclonal antibodies like Rituximab (Rituxan). Rituximab is also effective against other 5 indolent B-cell cancers, including marginal-zone lymphoma, WM, CLL and small lymphocytic lymphoma. A combination of Rituximab and chemotherapy agents is especially effective. A modified approach is radioimmunotherapy, wherein a monoclonal antibody is combined with a radioisotope particle, such as Iodine-131 tositumomab (Bexxar®) and Yttrium-90 ibritumomab tiuxetan (Zevalin®). In another example, Bexxar® is used in sequential treatment with CHOP.

Another immunotherapy example includes using cancer vaccines, which is based upon the genetic makeup of an individual patient’s tumor. A lymphoma vaccine example is GTOP-99 (MyVax®).

[0253] Another approach to treating mande cell lymphoma includes autologous stem cell transplantation coupled with high-dose chemotherapy.

[0254] Another approach to treating mande cell lymphoma includes administering protéasome inhibitors, such as Velcade® (bortezomib or PS-341), or andangiogenesis agents, such as thalidomide, especially in combination with Rituxan. Another treatment approach is administering drugs that lead to the dégradation of Bcl-2 protein and increase cancer cell sensitivity to chemotherapy, such as oblimersen (Genasense) in combination with other chemotherapeudc agents. Another treatment approach includes administering mTOR inhibitors, which can lead to inhibition of cell growth and even cell death; a non-limiting example is Temsirolimus (CCI-779), and Temsirolimus in combination with Rituxan®, Velcade® or other chemotherapeutic agents.

[0255] Other recent thérapies for MCL hâve been disclosed (Nature Reviews; Jares, P. 2007).

Non-limiting examples include Flavopiridol, PD0332991, R-roscovitine (Selicilib, CYC202), Styryl sulphones, Obatoclax (GX15-070), TRAIL, Anti-TRAIL DR4 and DR5 antibodies, Temsirolimus (CCI-779), Everolimus (RAD001), BMS-345541, Curcumin, Vorinostat (SAHA), Thalidomide, lenalidomide (Revlimid®, CC-5013), and Geldanamycin (17-AAG).

[0256] Non-limiting examples of other therapeutic agents used to treat Waldenstrom’s

Macroglobulinemia include perifosine, bortezomib (Velcade®), rituximab, sildenafil citrate (Viagra®), CC-5103, thalidomide, epratuzumab (hLL2- anti-CD22 humanized antibody), simvastatin, enzastaurin, campath-ΙΗ, dexamethasone, DT PACE, oblimersen, antineoplaston s/

A10, antineoplaston AS2-1, alemtuzumab, beta alethine, cyclophosphamide, doxorubicin hydrochloride, prednisone, vincristine sulfate, fludarabine, filgrastim, melphalan, recombinant interferon alfa, carmustine, cisplatin, cyclophosphamide, cytarabine, etoposide, melphalan, dolastatin 10, indium In 111 monoclonal antibody MN-14, yttrium Y 90 humanized epratuzumab, anti-thymocyte globulin, busulfan, cyclosporine, methotrexate, mycophenolate mofetil, therapeutic allogeneic lymphocytes, Yttrium Y 90 ibritumomab tiuxetan, sirolimus, tacrolimus, carboplatin, thiotepa, paclitaxel, aldesleukin, recombinant interferon alfa, docetaxel, ifosfamide, mesna, recombinant interleukin-12, recombinant interleukin-11, Bcl-2 family protein inhibitor ABT-263, denileukin diftitox, tanespimycin, everolimus, pegfilgrastim, vorinostat, alvocidib, recombinant flt3 ligand, recombinant human thrombopoietin, lymphokine-activated killer cells, amifostine trihydrate, aminocamptothecin, irinotecan hydrochloride, caspofungin acetate, clofarabine, epoetin alfa, nelarabine, pentostatin, sargramostim, vinorelbine ditartrate, WT-1 analog peptide vaccine, WT1 126-134 peptide vaccine, fenretinide, ixabepilone, oxaliplatîn, monoclonal antibody CD19, monoclonal antibody CD20, omega-3 fatty acids, mitoxantrone hydrochloride, octreotide acetate, tositumomab and iodine 1-131 tositumomab, motexafin gadolinium, arsenic trioxide, tipifarnib, autologous human tumor-derived HSPPC-96, veltuzumab, bryostatin 1, and PEGylated liposomal doxorubicin hydrochloride, and any combination thereof.

[0257] Non-limiting examples of other therapeutic agents used to treat diffuse large B-cell lymphoma (DLBCL) drug thérapies (Blood 2005 Abramson, J.) include cyclophosphamide, doxorubicin, vincristine, prednisone, anti-CD20 monoclonal antibodies, etoposide, bleomycin, many of the agents listed for Waldenstrom’s, and any combination thereof, such as ICE and R-ICE.

[0258] Non-limiting examples of therapeutic procedures used to treat Waldenstrom’s

Macroglobulinemia include peripheral blood stem cell transplantation, autologous hematopoietic stem cell transplantation, autologous bone marrow transplantation, antibody therapy, biological therapy, enzyme inhibitor therapy, total body irradiation, infusion of stem cells, bone marrow ablation with stem cell support, in wiro-treated peripheral blood stem cell transplantation, umbilical cord blood transplantation, immunoenzyme technique, pharmacological study, low30 LET cobalt-60 gamma ray therapy, bleomycin, conventional surgery, radiation therapy, and nonmyeloablative allogeneic hematopoietic stem cell transplantation.

s/

[0259] Non-limiting examples of other therapeutic agents used to treat Chrome Lymphocytic Leukemia (Spectrum, 2006, Fernandes, D.) include Chlorambucii (Leukeran), Cyclophosphamide (Cyloxan, Endoxan, Endoxana, Cyclostin), Fludarabine (Fludara), Pentstatin (Nipent), Cladribine (Leustarin), Doxorubicin (Adriamycin®, Adriblastine), Vincristine (Oncovin), Prednisone, Prednisolone, AJemtuzumab (Campath, MabCampath), many of the agents listed for Waldenstrom’s, and combination chemotherapy and chemoimmunotherapy, including the common combination regimen: CVP (cyclophosphamide, vincristine, prednisone); R-CVP (rituximab-CVP); ICE (iphosphamide, carboplatin, etoposide); R-ICE (rituximab-ICE); FCR (fludarabine, cyclophosphamide, rituximab); and FR (fludarabine, rituximab).

[0260] In certain embodiments, the method comprises administering in addition to a compound of I or II to said patient, a therapeutically effective amount of at least one therapeutic agent and/or therapeutic procedure selected to treat said cancer in said patient. In certain embodiments, the method comprises administering in addition to a compound of I or II to said patient, a therapeutically effective amount of a combination of therapeutic agents selected from the group consisting of a) bendamustine; b) rituximab; c) bendamustine and rituximab; d) ofatumumab; and e) lenalidomide.

[0261] The compounds of the invention may be formulated for administration to animal subject using commonly understood formulation techniques well known in the art. Formulations which are suitable for particular modes of administration and for the compounds of formula A 20 may be found in Remington’s Pharmaceutical Sciences, latest édition, Mack Publishing Company, Easton, PA.

[0262] The compounds of the invention may be prepared in the form of prodrugs, Le., protected forms which release the compounds of the invention after administration to the subject. Typically, the protecting groups are hydrolyzed in body fluids such as in the bloodstream thus releasing the active compound or are oxidized or reduced In vivo to release the active compound. A discussion of prodrugs is found in Smith and Williams Introduction to the Principles of Drug Design. Smith, H.J.; Wright, 2^nd ed., London (1988).

[0263] A compound of the présent invention can be administered as the neat chemical, but it is typically préférable to administer the compound in the form of a pharmaceutical composition or formulation. Accordingly, the présent invention also provides pharmaceutical compositions that comprise a compound of formula A and a biocompatible pharmaceutical carrier, adjuvant, or vehicle. The composition can include the compound of Formula A as the only active moiety or

in combination with other agents, such as oligo- or polynucleotides, oligo- or polypeptides, drugs, or hormones mixed with excipient(s) or other pharmaceutically acceptable carriers. Carriers and other ingrédients can be deemed pharmaceutically acceptable insofar as they are compatible with other ingrédients of the formulation and not deleterious to the récipient thereof. [0264] The pharmaceutical compositions are formulated to contain suitable pharmaceutically acceptable carriers, and can optionally comprise excipients and auxiliaries that facilitate processing of the active compounds into préparations that can be used pharmaceutically. The administration modality will generally détermine the nature of the carrier. For example, formulations for parentéral administration can comprise aqueous solutions of the active compounds in water-soluble form. Carriers suitable for parentéral administration can be selected from among saline, buffered saline, dextrose, water, and other physiologically compatible solutions. Preferred carriers for parentéral administration are physiologically compatible buffers such as Hank’s solution, Ringer’s solution, or physiologically buffered saline. For tissue or cellular administration, pénétrants appropriate to the particular barrier to be permeated are used in the formulation. Such pénétrants are generally known in the art. For préparations comprising proteins, the formulation can include stabilizing materials, such as polyols (e.g., sucrose) and/or surfactants (e.g., nonionic surfactants), and the like.

[0265] Altematively, formulations for parentéral use can comprise dispersions or suspensions of the active compounds prepared as appropriate oily injection suspensions. Suitable lipophilie solvents or vehicles include fatty oils, such as sesame oil, and synthetic fatty acid esters, such as ethyl oleate or triglycérides, or liposomes. Aqueous injection suspensions can contain substances that increase the viscosity of the suspension, such as sodium carboxymethylcellulose, sorbitol, or dextran. Optionally, the suspension also can contain suitable stabîlizers or agents that increase the solubility of the compounds to allow for the préparation of highly concentrated solutions. Aqueous polymers that provide pH-sensitive solubilization and/or sustained release of the active agent also can be used as coatings or matrix structures, e.g., methacrylic polymers, such as the Eudragit® sériés available from Rohm America Inc. (Piscataway, N.J.). Emulsions, e.g., oil-in-water and water-in-oil dispersions, also can be used, optionally stabilized by an emulsifying agent or dispersant (surface active materials; surfactants). Suspensions can contain suspending agents such as ethoxylated isostearyl alcohols, polyoxyethlyene sorbitol and sorbitan esters, microcrystalline cellulose, aluminum metahydroxide, bentonite, agar-agar, gum tragacanth, and mixtures thereof.

[0266] Liposomes containing the active compound of Formula A also can be employed for parentéral administration. Liposomes generally are derived from phospholipids or other lipid substances. The compositions in liposome form also can contain other ingrédients, such as stabilizers, preservatives, excipients, and the like. Preferred lipids include phospholipids and phosphatidyl cholines (lecithins), both natural and synthetic. Methods of forming liposomes are known in the art. See,. e.g., Prescott (Ed.), Methods in Cell Biology. Vol. XIV, p. 33, Academie Press, New York (1976).

[0267] The pharmaceutical compositions comprising the compound of Formula A in dosages suitable for oral administration can be formulated using pharmaceutically acceptable carriers well known in the art. The préparations formulated for oral administration can be in the form of tablets, pills, capsules, cachets, dragees, lozenges, liquids, gels, syrups, slurries, élixirs, suspensions, or powders. To illustrate, pharmaceutical préparations for oral use can be obtained by combining the active compounds with a solid excipient, optionally grinding the resulting mixture, and processing the mixture of granules, after adding suitable auxiliaries if desired, to obtain tablets or dragée cores. Oral formulations can employ liquid carriers similar in type to those described for parentéral use, e.g., buffered aqueous solutions, suspensions, and the like.

[0268] Preferred oral formulations include tablets, dragees, and gelatin capsules. These préparations can contain one or excipients, which include, without limitation:

a) diluents, such as sugars, including lactose, dextrose, sucrose, mannitol, or sorbitol;

b) binders, such as magnésium aluminum silicate, starch from corn, wheat, rice, potato, etc.;

c) cellulose materials, such as methylcellulose, hydroxypropylmethyl cellulose, and sodium carboxymethylcellulose, polyvinylpyrrolidone, gums, such as gum arabïc and gum tragacanth, and proteins, such as gelatin and collagen;

d) disintegrating or solubilizing agents such as cross-linked polyvinyl pyrrolidone, starches, agar, alginic acid or a sait thereof, such as sodium alginate, or effervescent compositions;

e) iubricants, such as silica, talc, stearic acid or its magnésium or calcium sait, and polyethylene glycol;

f) flavorants and sweeteners;

g) colorants or pigments, e.g., to identîfy the product or to characterize the quantity (dosage) of active compound; and

h) other ingrédients, such as preservatives, stabilizers, swelling agents, emulsifying agents, solution promoters, salts for regulating osmotic pressure, and buffers.

[0269] In some preferred oral formulations, the pharmaceutical composition comprises at least one of the materials from group (a) above, or at least one material from group (b) above, or at least one material from group (c) above, or at least one material from group (d) above, or at least one material from group (e) above. Preferably, the composition comprises at least one material from each of two groups selected from groups (a)-(e) above.

[0270] Gelatin capsules include push-fit capsules made of gelatin, as well as soft, sealed capsules made of gelatin and a coating such as glycerol or sorbitol. Push-fit capsules can contain the active ingredient(s) mixed with fillers, binders, lubricants, and/or stabilizers, etc. In soft capsules, the active compounds can be dissolved or suspended in suitable fluids, such as fatty oils, liquid paraffin, or liquid polyethylene glycol with or without stabilizers.

[0271] Dragée cores can be provided with suitable coatîngs such as concentrated sugar solutions, which also can contain gum arabic, talc, polyvinyl pyrrolidone, carbopol gel, polyethylene glycol, and/or titanium dioxide, lacquer solutions, and suitable organic solvents or solvent mixtures.

[02721 The pharmaceutical composition can be provided as a sait of the active compound.

Salts tend to be more soluble in aqueous or other protonic solvents than the corresponding free acid or base forms. Pharmaceutically acceptable salts are well known in the art. Compounds that contain acidic moieties can form pharmaceutically acceptable salts with suitable cations. Suitable pharmaceutically acceptable cations include, for example, alkali métal (e.g., sodium or potassium) and alkaline earth (e.g., calcium or magnésium) cations.

[0273] Compounds of structural formula (A) that contain basic moieties can form pharmaceutically acceptable acid addition salts with suitable acids. For example, Berge, et al., describe pharmaceutically acceptable salts in detail in J Phartn Set, 66:1 (1977). The salts can be prepared in situ during the final isolation and purification of the compounds of the invention or separately by reacting a free base function with a suitable acid.

[0274] Représentative acid addition salts include, but are not limited to, acetate, adipate, alginate, citrate, aspartate, benzoate, benzenesulfonate, bisulfate, butyrate, camphorate, camphorolsulfonate, digluconate, glycérophosphate, hemisulfate, heptanoate, hexanoate,

fumarate, hydrochloride, hydrobromide, hydroiodide, 2-hydroxyethanesuIfonate (isothionate), Iactate, maleate, methanesulfonate or sulfate, nicotinate, 2-naphthalenesulfonate, oxalate, pamoate, pectinate, persulfate, 3-phenylpropionate, picrate, pivalate, propionate, succinate, tartrate, thiocyanate, phosphate or hydrogen phosphate, glutamate, bicarbonate, p-toluenesulfonate, and undecanoate. Examples of acids that can be employed to form pharmaceutically acceptable acid addition salts include, without limitation, such inorganic acids as hydrochloric acid, hydrobromic acid, sulfuric acid, and phosphoric acid, and such organic acids as oxalic acid, maleic acid, succinic acid, and citric acid.

[0275] Basic nitrogen-containing groups can be quaternized with such agents as lower alkyl halides such as methyl, ethyl, propyl, and butyl chlorides, bromides and iodides; dialkyl sulfates like dimethyl, diethyl, dibutyl, and diamyl sulfates; long chain alkyl halides such as decyl, lauryl, myristyl, and stearyl chlorides, bromides, and iodides; arylalkyl halides such as benzyl and phenethyl bromides; and others. Products having modified solubility or dispersibility are thereby obtained.

[0276] Compositions comprising a compound of the invention formulated in a pharmaceutical acceptable carrier can be prepared, placed in an appropriate container, and labeled for treatment of an indicated condition. Accordingly, there also is contemplated an article of manufacture, such as a container comprising a dosage form of a compound of the invention and a label containing instructions for use of the compound. Kits are also contemplated under the invention. For example, the kit can comprise a dosage form of a pharmaceutical composition and a package insert containing instructions for use of the composition in treatment of a medical condition. In either case, conditions indicated on the label can include treatment of inflammatory disorders, cancer, etc.

Methods of administration [0277] Pharmaceutical compositions comprising a compound of formula A can be administered to the subject by any conventional method, including parentéral and enterai techniques. Parentéral administration modalities include those in which the composition is administered by a route other than through the gastrointestinal tract, for example, intravenous, intraarterial, intraperitoneal, intramedullarly, intramuscular, intraarticular, intrathecal, and intraventricular injections. Enterai administration modalities include, for example, oral (including buccal and sublingual) and rectal administration. Transepithelial administration modalities include, for example, transmucosal administration and transdermal administration.

Transmucosal administration includes, for example, enterai administration as well as nasal, inhalation, and deep lung administration; vaginal administration; and rectal administration. Transdermal administration includes passive or active transdermal or transcutaneous modalities, including, for example, patches and iontophoresis devices, as well as topical application of pastes, salves, or ointments. Parentéral administration also can be accomplîshed using a highpressure technique, e.g., POWDERJECT™.

[0278] Surgical techniques include implantation of depot (réservoir) compositions, osmotic pumps, and the like. A preferred route of administration for treatment of inflammation can be local or topical delivery for localized disorders such as arthritis, or systemic delivery for dîstributed disorders, e.g., intravenous delivery for reperfusion injury or for systemic conditions such as septicemia. For other diseases, including those involving the respiratory tract, e.g., chronic obstructive pulmonary disease, asthma, and emphysema, administration can be accomplîshed by inhalation or deep lung administration of sprays, aérosols, powders, and the like.

[0279] In some foregoing embodiments, the compound of formula A is administered before, during, or after administration of chemotherapy, radiotherapy, and/or surgery. The formulation and route of administration chosen will be tailored to the individual subject, the nature of the condition to be treated in the subject, and generally, the judgment of the attending practitioner. [0280] The therapeutic index of the compound of formula A can be enhanced by modifying or derivatizing the compounds for targeted delivery to cancer cells expressing a marker that identifies the cells as such. For example, the compounds can be linked to an antibody that recognizes a marker that is sélective or spécifie for cancer cells, so that the compounds are brought into the vicinity of the cells to exert their effects locally, as previously described (see for example, Pietersz, et al., ImmunolRev, 129:57 (1992); Trail, étal., Science, 261:212 (1993); and Rowlinson-Busza, et al., Curr Opin Oncol, 4:1142 (1992)). Tumor-directed delivery of these compounds enhances the therapeutic benefit by, inter alia, minimizing potential nonspecific toxicities that can resuit from radiation treatment or chemotherapy. In another aspect, the compound of formula A and radioisotopes or chemotherapeutic agents can be conjugated to the same anti-tumor antibody.

[0281] The characteristics of the agent itself and the formulation of the agent can influence the physical state, stability, rate of In vivo release, and rate of In vivo clearance of the administered agent. Such pharmacokinetic and pharmacodynamie information can be collected through

preclinical in vitro and In vivo studies, later confirmed in humans during the course of clinical trials. Thus, for any compound used in the method of the invention, a therapeutically effective dose can be estimated initially from biochemical and/or cell-based assays. Then, dosage can be formulated in animal models to achieve a désirable circulating concentration range that modulâtes expression or activity of a particular PI3K isoform or combination of isoforms. As human studies are conducted, further information will emerge regarding the appropriate dosage levels and duration of treatment for various diseases and conditions.

[0282] Although compounds of the invention are well tolerated, an example of a limit to the treatment dosage is eievated liver function tests (LFT). LFT involve standard clinical biochemistry tests on the patient’s sérum or plasma to provide information about the state of a patient’s liver. Levels, such as alanine transaminase, aspartate transaminase, alkaline phosphatase, bilirubin, and gamma glutamyl transpeptidase, that are outside the normal range can signal possible liver toxicity. Dosing of the therapeutic compound can be adjusted to avoid or reduce eievated liver function test values and subséquent potential for liver toxicity. For instance, a subject may be administered escalating doses of a compound. At a certain dose amount, the subject begins to develop eievated LFT levels outside a normal range, signaling potential liver toxicity at that dosage. In response, the dosage may be reduced to an amount such that LFT levels are reduced to an acceptable range as judged by the treating physician, e.g. a level that is in the range normal for the subject being treated, or within about 25% to 50% of normal. Therefore, liver function tests can be used to titrate the administration dosage of a compound.

[0283] Toxicity and therapeutic effîcacy of such compounds can be determined by standard pharmaceutical procedures in cell cultures or experimental animais, e.g., for deteimining the LD50 (the dose léthal to 50% of the population) and the ED50 (the dose therapeutically effective in 50% of the population). The dose ratio between toxic and therapeutic effects is the “therapeutic index,” which typically is expressed as the ratio LD50/ED50. Compounds that exhibit large therapeutic indices, i.e., the toxic dose is substantially higher than the effective dose, are preferred. The data obtained from such cell culture assays and additional animal studies can be used in formulating a range of dosage for human use. The dosage of such compounds lies preferably within a range of circulating concentrations that include the ED50 with little or no toxicity.

~ 51 [0284] Dosage may be limited by treatment-related toxicity symptoms. Such symptoms besîdes elevated liver function tests include anémia, vision blurring, diarrhea, vomiting, fatigue, mucositis, peripheral edema, pyrexia, peripheral neuropathy, pleural effusion, night sweats, and orthopnea, or a combination thereof. At a certain dose amount, if the subject develops intolérable levels of such symptoms, the dosage may be reduced such that the adverse event is eliminated and no longer adverse or reduced to an acceptable level as judged by a treating physician.

[0285] Another considération in determining the appropriate dose of compound for a patient is the desired concentration circulating in the blood plasma. In a particular embodiment, the concentration of compound in the blood is between 40-3,000 ng/mL over a 12 hour period from the time of administration. In another particular embodiment, the concentration of compound in the blood is between 75-2,000 ng/mL over a 12 hour period from the time of administration. In another particular embodiment, the concentration of compound in the blood is between 5002,000 ng/mL over a 12 hour period from the time of administration. In a preferred embodiment, the concentration of compound in the blood is between 40-3,000 ng/mL over a 12 hour period from the time of administration, wherein the compound is a formula of I, I”, II, or II” and is orally administered in an amount of about 50 mg, 100 mg, 150 mg, or 200 mg. In a preferred embodiment, the concentration of compound in the blood is between 40-3,000 ng/mL over a 12 hour period from the time of administration, wherein the compound is a formula of I and is orally administered in an amount of about 50 mg, 100 mg, 150mg, or 200 mg. In a preferred embodiment, the concentration of compound in the blood is between 40-3,000 ng/mL over a 12 hour period from the time of administration, wherein the compound is a formula of II and is orally administered in an amount of about 50 mg, 100 mg, 150 mg, or 200 mg. In some of the foregoing embodiments, the maximum concentration in the blood plasma is achieved within two 25 hours of administration.

[0286] In certain embodiments, the dosage of the compound of Formula I or II is selected to produce a plasma concentration of drug of about 10 nM or higher over a period of 8 to 12 hours, on average, and to provide a peak plasma concentration of about 500 nM or higher, preferably about 1000 nM or higher. In certain embodiments, the dosage of the compound of Formula I or 30 II is selected to produce a plasma concentration of drug of about 100 nM or higher over a period of 8 to 12 hours, on average, and to provide a peak plasma concentration of about 500 nM or higher, preferably about 1000 nM or higher. In certain embodiments, the dosage of the

compound of Formula I or II is selected to produce a plasma concentration of drug of about 200 nM or higher over a period of 8 to 12 hours, on average, and to provide a peak plasma concentration of about 500 nM or higher, preferably about 1000 nM or higher.

[0287] In certain embodiments, the dosage of the compound of formula I or II is selected to produce a plasma concentration wherein the trough concentration of the compound is in the range where a therapeutic effect, such as apoptosis of cancer cells, is observed. In certain embodiments, the dosage of the compound of formula I or II is selected to produce a trough plasma concentration at or higher than the EC50 ΡΙ3Κδ isoform activation in blood plasma. In certain embodiments, the dosage of the compound of formula I or II is selected to produce an 10 trough blood concentration above the EC50 level for ΡΙ3Κδ activation and below the level for EC50 ΡΙ3Κγ activation in a cell during a period of at least 12 hours from compound administration. For instance, if the EC50 value for PI3K δ basophil activation is 65 nM and the EC50 value for PI3K γ basophil activation is 1100 nM in whole blood plasma, then the dosage of the compound selected provides a trough plasma concentration of the compound between 60 nM 15 and 1100 nM during a period of 8-12 hours from compound administration. Similarly, a dosage can be selected to produce an trough blood concentration above the EC50 level for PI3K.Ô basophil activation and below the EC50 level for PI3K -α, -β or -γ basophil activation. The EC50 values for the PI3K isoform activation or inhibition In vivo can be determined by a person having ordinary skill in the art. In alternative embodiments, the upper range of the trough concentration of the drug may exceed and is not limited by the EC50 value of the PI3K -γ, -a, or -β isoform in blood plasma. Moreover, the blood concentration range of the drug is at a level which is therapeutically bénéficiai in treating the hématologie malignancy, while minimizing undesirable side effects.

[0288] For instance, while being delta-selective, the compounds can exhibit sufficient activity 25 on pl 10γ to be clinically useful, i.e., to be effective on a cancer that relies upon pl 10γ for signaling, because a plasma level above the effective dosage for inhibition of pl 10γ can be achieved while still being sélective relative to other isoforms, particularly the alpha isoform. Thus, in some embodiments, the dosage of the compound is selected to produce a blood concentration effective for selectively inhibiting pl 10δ and pl 10γ.

[0289] In some embodiments, the dosage of the compound provides a trough blood plasma concentration between 65 nM and 1100 nM during a period of 8 to 12 hours from compound

administration. In some foregoing embodiments, the period is at least 12 hours from compound administration.

[0290] In a particular embodiment, the compound is adminîstered in a therapeutically effective amount.

[0291] In a particular embodiment, the compound is adminîstered at a dose of 20-500 mg/day.

In a particular embodiment, the compound is adminîstered at a dose of 50-250 mg/day.

[0292] In a particular embodiment, the compound is adminîstered at a dose of 25 to 150 mg per dose, and two doses are adminîstered per day (e.g., BID dosing with 25 to 150 mg doses). In a preferred embodiment, a subject is treated with 50 mg to 100 mg of a compound of formula A 10 twice per day. In other preferred embodiment, a subject is treated with 150 mg of a compound of formula A twice per day.

[0293] In a particular embodiment, the method comprises administering to said patient an initial daily dose of 20-500 mg of the compound and increasing said dose by incréments until clinical efficacy is achieved. Incréments of about 25,50,100, or 150 mg can be used to increase 15 the dose. The dosage can be increased daily, every other day, twice per week, or once per week.

[0294] In a particular embodiment, the method comprises continuing to treat said patient by administering the same dose of the compound at which clinical efficacy is achieved or reducing said dose by incréments to a level at which efficacy can be maintained.

[0295] In a particular embodiment, the method comprises administering to said patient an initial daily dose of 20-500 mg of the compound and increasing said dose to a total dosage of 50400 mg per day over at least 6 days. Optionally, the dosage can be further increased to about 750 mg/day.

[0296] In a particular embodiment, the compound is adminîstered at least twice daily.

[0297] In a particular embodiment, the compound is adminîstered orally, intravenously or by inhalation. Preferably, the compound is adminîstered orally. In some embodiments, it is adminîstered orally at a dosage of about 50 mg BID, at a dosage of about 100 mg BID, or at a dosage of about 150 mg BID.

[0298] For the methods of the invention, any effective administration regimen regulating the timing and sequence of doses can be used. Doses of the agent preferably include pharmaceutical 30 dosage units comprising an effective amount of the agent. As used herein, “effective amount” refers to an amount sufficient to modulate PI3K5 expression or activity and/or dérivé a measurable change in a physiological parameter of the subject through administration of one or

more of the pharmaceutical dosage units. “Effective amount” can also refer to the amount required to ameliorate a disease or disorder in a subject.

[0299] Suitable dosage ranges for the compounds of formula A vary according to these considérations, but in general, the compounds are administered in the range of

10.0 pg/kg-15 mg/kg of body weight; 1.0 pg/kg-10 mg/kg of body weight, or 0.5 mg/kg-5 mg/kg of body weight. For a typical 70-kg human subject, thus, the dosage range is from

700 pg-1050 mg; 70 pg-700 mg; or 35 mg-350 mg per dose, and two or more doses may be administered per day. Dosages may be higher when the compounds are administered orally or transdermally as compared to, for example, i.v. administration. The reduced toxicity of a compound of formula A, permits the therapeutic administration of relatively high doses. In some of the foregoing embodiments, oral administration of up to 750 mg/day of a compound of the invention is suitable. In some of the foregoing embodiments, a compound of formula A is administered at a dose of 50 mg BID. In some of the foregoing embodiments, a compound of formula A is administered at a dose of 100 mg BID. In some of the foregoing embodiments, a compound of formula A is administered at a dose of 150 mg BID. In some of the foregoing embodiments, a compound of formula A is administered at a dose of 200 mg BID. In some of the foregoing embodiments, a compound of formula A is administered at a dose of 350 mg BID. In spécifie embodiments, for treatment of leukemias, lymphomas and multiple myeloma, a dosage of about 50-350 mg per dose, administered OTally once or preferably twice per day, is often suitable.

[0300] In some of the foregoing embodiments, oral administration of up to 750 mg/day of compound I” or II” is suitable. In some of the foregoing embodiments, a compound of formula I” or II” is administered at a dose of 50 mg BID. In some of the foregoing embodiments, a compound of formula I” or II” is administered at a dose of 100 mg BID. In some of the foregoing embodiments, a compound of formula I” or II” is administered at a dose of 150 mg BID. In some of the foregoing embodiments, a compound of formula I” or II” is administered at a dose of 200 mg BID. I In some of the foregoing embodiments, a compound of formula I” or II” is administered at a dose of 350 mg BID. In some of the foregoing embodiments, for treatment of leukemias, lymphomas and multiple myeloma, a dosage of about 50-350 mg per dose of a compound of formula I” or II”, administered orally once or preferably twice per day, is often suitable.

[0301] The compounds may be administered as a single bolus dose, a dose over time, as in i.v. or transdermal administration, or in multiple dosages.

[0302] Dosing is continued for at least one cycle. In some embodiments, the cycle is at least seven days. In some embodiments, the cycle is about 28 days. In some embodiments, dosing is continued for about 28 days and is then discontinued for at least 7 days. In some embodiments, a complété cycle is continuous daily dosing for 28 days. Evaluation of a clinical response in the patient can be measured after each cycle. The clinical results can be used to make a decision to increase, decrease, discontinue or maintain the dosage.

[0303] Depending on the route of administration, a suitable dose can be calculated according to body weight, body surface area, or organ size. The final dosage regimen will be determined by the attending physician in view of good medical practice, considering various factors that modify the action of drugs, e.g., the agent’s spécifie activity, the identity and severity of the disease state, the responsiveness of the patient, the age, condition, body weight, sex, and diet of the patient, and the severity of any infection. Additional factors that can be taken into account include time and frequency of administration, drug combinations, reaction sensitivities, and tolerance/response to therapy. Further refinement of the dosage appropriate for treatment involving any of the formulations mentioned herein is done routinely by the skilled practitioner without undue expérimentation, especially in light of the dosage information and assays disclosed, as well as the pharmacokinetic data observed in human clinical trials. Appropriate dosages can be ascertained through use of established assays for determining concentration of the agent in a body fluid or other sample together with dose response data.

[0304] The frequency of dosing will dépend on the pharmacokinetic parameters of the compound of Formula A and the route of administration. Dosage and administration are adjusted to provide sufficient levels of the active moiety or to maintain the desired effect.

Accordingly, the pharmaceutical compositions can be administered in a single dose, multiple discrète doses, continuous infusion, sustained release depots, or combinations thereof, as required to maintain desired minimum level of the compound. Short-acting pharmaceutical compositions (Le., short half-life) can be administered once a day or more than once a day (e.g., two, three, or four times a day). Long acting pharmaceutical compositions might be administered every 3 to 4 days, every week, or once every two weeks. Pumps, such as subcutaneous, intraperitoneal, or subdural pumps, can be preferred for continuous infusion.

[0305] Subjects that will respond favorably to the method of the invention include medical and veterinary subjects generally, including human patients. Among other subjects for whom the methods of the invention is useful are cats, dogs, large animais, avians such as chickens, and the like. In general, any subject who would benefit from a compound of formula A is appropriate for administration of the invention method. In some foregoing embodiments, the patient has a cytogenetic characteristic of del(17p) or del(llq). In some foregoing embodiments, the patient has a lymphadenopathy. In some foregoing embodiments, the use of compound I, i”, II, or II” reduces the size of a lymphadenopathy in a patient. In some foregoing embodiments, the use of compound I, I”, II, or II” reduces the size of a lymphadenopathy after one cycle of treatment. In some foregoing embodiments, the use of compound I, I”, II, or II” reduces the size of a lymphadenopathy by at least 10 % after one cycle of treatment. In some foregoing embodiments, the use of compound I, I”, II, or II” reduces the size of a lymphadenopathy by at least 25 % after one cycle of treatment. In some foregoing embodiments, the use of compound I, I”, II, or II” reduces the size of a lymphadenopathy by at least 30 % after one cycle of treatment. In some foregoing embodiments, the use of compound I, I”, Π, or II” reduces the size of a lymphadenopathy by at least 40 % after one cycle of treatment. In some foregoing embodiments, the use of compound I, I”, II, or II” reduces the size of a lymphadenopathy by at least 50 % after one cycle of treatment. In some foregoing embodiments, the use of compound I, I”, II, or II” reduces the size of a lymphadenopathy by at least 75 % after one cycle of treatment.

[0306] In one aspect, the invention provides a method of treating a condition, comprising administering a compound of formula I, II or a pharmaceutically acceptable sait thereof and one or more therapeutic agents to a subject in need of such treatment, wherein the condition is a cancer. In one embodiment, the one or more therapeutic agents is a protéasome inhibitor. The one or more therapeutic agents may include bendamustine, rituximab, ofatumumab, and/or lenalidomide. In another embodiment, the one or more therapeutic agent includes bendamustine, rituximab, or a combination of these two therapeutic agents. In yet another embodiment, the one or more therapeutic agent includes ofatumumab. In yet another embodiment, the one or more therapeutic agent includes lenalidomide.

[0307] Suitable dosage ranges for the one or more therapeutic agents may vary, but in general, the one or more therapeutic agents are administered in the range of 50 mg/m² and 1,500 mg/m². In one embodiment, bendamustine is administered in the range of 50 mg/m² and 150 mg/m². In

another embodiment, rituximab is administered in the range of 300 mg/m² and 400 mg/m². In another embodiment, ofatumumab is administered in the range of 300 mg/m² and 1,500 mg/m². [0308] Dosing of the one or more therapeutic agents in combination with a compound of formula A may be continued for at least one cycle. In some embodiments, dosing of the one or more therapeutic agents in combination with a compound of formula A may be continued for at least seven days. In other embodiments, dosing of the one or more therapeutic agents in combination with a compound of formula A may be continued for about 28 days. In some embodiments, a compound of formula A and one or more therapeutic agents are each administered at least once during at least one cycle. The one or more therapeutic agents may be administered to the subject in the same or different cycles as the administration of the compound. In some embodiments, the one or more therapeutic agents are administered to the subject on at least the first and second days of at least one cycle. In other embodiments, the one or more therapeutic agents are administered to the subject weekly. Evaluation of a clinical response in the patient can be measured after each cycle. The clinical results can be used to make a decision to increase, decrease, discontinue or maintain the dosage.

[0309] In some of the foregoing embodiments, the condition is a hématologie maiignancy. In preferred embodiments, the condition is selected from the group consisting of multiple myeloma, acute lymphocytic leukemia, acute myeloid leukemia, chronic lymphocytic leukemia, B-cell lymphoma, diffuse large B-cell lymphoma, B-cell ALL, T-cell ALL and Hodgkin’s lymphoma.

[0310] In preferred embodiments, the compound is substantially comprised of the Senantiomer. In spécifie embodiments, the compound comprises at least 95% of the Senantiomer. In some of the foregoing embodiments, the administration of said compound and therapeutic agent provides a synergistic benefit superior to results obtained without the combination of the compound and therapeutic agent.

[0311] The following examples are offered to illustrate but not to limit the invention. In the examples below, references to the ‘compound of formula Γ or ‘compound Γ refer to the Senantiomer shown here, and samples used for these Examples exhibited a 98.2%ee as measured by chiral HPLC methods:

(S-enantiomer) [0312] In addition, an analysis of this compound reveals the following characteristics of the material:

f'!.? '	® t Resylt
Appearance	Slightly off-white powder
1H-NMR	Spectrum conforms to the reference
HPLC Assay	98.1 % (Anhydrous, solvent-free basis)
Chiral Purity (HPLC)	98.2 % ee

Residual on Ignition	0.11 %
Infrared Spectroscopy (FTIR)	Spectrum in agreement with the reference
13C-NMR	Spectrum conforms to the reference
Particle Size Analysis	Médian diameter: 11.3 pm
Water (Couiometric Karl Fischer)	0.56 %

PrààertÊfij'Twt · ·?:’£
Elemental Analysis % C, H, F, N		Expected	Found
%c	63.3	63.5
%H	4.4	4.4
%N	23.5	23.1
%F	4.5	4.5

Example 1

Inhibition of Cell Growth in MM Cells [0313] This example demonstrates the compound of formula I inhibîts the cellular growth stimulatory effects of cytokines (IGF-1 and IL-6) in multiple myeloma (MM) cells. LB cells (Myelomonocytic myeloma cell line) were cultured for 48h with control media; with the compound of formula I, in the presence or absence of either IL-6 or IGF-1. The inhibitory effect of the compound of formula I on MM cell growth was assessed by measuring 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrasodium bromide (MTT; Chemicon International) dye absorbance. Cells were pulsed with 10 pL of 5 mg/mL MTT to each well for the last 4 hours of 48-hour cultures, followed by 100 pL isopropanol containing 0.04 N HCl.

Absorbance was measured at 570/630 nm using a spectrophotometer (Molecular Devices). A summary of the results is shown in Figure 1. Exposure of 0.625 pM-2.5 pM of Compound I inhibîts MM cell growth even in the presence of cell growth stimulatory cytokines.

Example 2

Effect of BMSC on Cytotoxicity [0314] This example demonstrates Bone Marrow Stromal Cells (BMSCs) do not protect against compound I-induced LB cell cytotoxicity. LB cells were cultured with control media, and with the compound of formula I for 48 hours, in the presence or absence of BMSCs. Cell prolifération was assessed using [³H]-thymidine uptake assay. Ail data represent mean (± SD) of triplicate experiment. A summary of the results is shown in Figure 2. LB cell growth is reduced after exposure to 0.625 pM-10 pM of compound I even in the presence of BMSC.

Example 3

Effect of Compound on Apoptosis of CLL Cells [0315] This example demonstrates the compound of formula I induces apoptosis in patient chronic lymphocytic leukemia (CLL) cells. Peripheral blood was obtained from patients with BCLL through the CLL Research Consortium from Ohio State University. Primary CD19positive cells were isolated using Rosette-Sep (StemCell Technologies). Cells were maintained in RPMI1640 (Invitrogen) suppiemented with 10% heat-inactivated fêtai bovine sérum, mmol/L L-glutamine, and penicillin (100 units/mL)/streptomycin (100 pg/mL; Invitrogen) at

37°C, 5% CO2, and high humidity. After incubation with the compound of formula I or medium for 96 hours, 5 x 10⁵ cells were washed with PBS and then resuspended in binding buffer (10 mmol/L HEPES/NaOH, pH 7.4,150 mmol/L NaCl 5 mmol/L KC1,1 mmol/L MgCh,

1.8 mmol/L CaCh) containing 2 pL of Annexin V-FITC stock (BioWhittaker, Inc) and 10 pL of

20 pg/mL PI (Sigma). After incubation for 10 minutes at room température in a light-protected area, the specimens were quantified by flow cytometry on a FACScan™ (Becton Dickinson). [0316] Treatment of CLL patient cells with compound I results in apoptosis and the resuit appears to be dose-dependent, as seen in Figure 3.

[0317] Compound I induced apoptosis was seen in CLL cells from poor prognosis patients, as 10 the data indicates in Figure 19.

[0318] Compound I induced apoptosis was also seen to be effective in CLL cells from refractory/relapsed patients as shown in Figure 20.

Example 4

Effect of compound in ALL cell lines [0319] This example demonstrates the compound of formula I results in a réduction of Akt phosphorylation and a decrease in cellular prolifération accompanied by cell death in both ΤΑΓΕ and B-ALL (Acute Lymphoblastic Leukemia) leukemic cell lines. Viability assays of cell lines were performed using the AlamarBlue assay (Invitrogen). Cells (1 x 10⁶ per well) in a volume of 100 pL were placed in a 96-well flat-bottom plate and the compound of formula I (100 pL per well at 2x final concentration) or medium alone was added to the plates. Ail were performed in quadruplicate. Cells were incubated for fixed times (48 hours). After the incubation, 10 pL AlamarBlue® was added to each well. Cells were incubated for 4 hours and the optical density at 530-560 nm was obtained using a SpectraMax® M5 plate reader 2001. Cell viability was expressed as a percentage of absorption between treated cells/control sample.

These results are summarized in the table shown in Figure 4. Exposure to compound I resuit in substantial réduction in cellular viability in a variety ALL cell lines as well as réduction in Akt phosphorylation.

Example 5

Effect of Compound on ALL Cell Cycle [0320] This example demonstrates treatment of the acute lymphoblastîc leukemia (ALL) cell line CCRF-SB with the compound of formula I results in G0/G1 cell cycle arrest.

Représentative fluorescence-activated cell sorting (FACS) analysis of propidium iodide-stained CCRF-SB cells under normal growth conditions, and growth in the presence of the compound of formula I. The average percentage of cells in G₀-Gi, S, and G₂-M phases is calculated in the table below the histographs. Results are shown in Figure 5.

Example 6

Inhibition of Prolifération of Breast Cancer Cells [0321] This example demonstrates the compound of formula I inhibits prolifération of breast cancer cell lines. T47D and HS-578T cell lines were grown in the presence of sérum plus the indicated concentrations of the compound of formula I. Prolifération was measured in triplicate wells by AlamarBlue® assay (Invitrogen) 96-weIl plates. Results of prolifération assays are expressed as the mean cellular percentage values and shown in Figure 6.

Example 7

Inhibition of Prolifération of Ovarian Cancer Cell Lines [0322] This example demonstrates the compound of formula I inhibits prolifération of ovarian cancer cell lines. IGROV-1 and OVCAR-3 cell lines were grown in the presence of sérum plus 20 the indicated concentrations of the compound of formula 1. Prolifération was measured in triplicate wells by AlamarBlue assay (Invitrogen) 96-well plates. Results of prolifération assays are expressed as the mean cellular percentage values and are shown in Figure 7.

Example 8

Réduction of Akt Phosphorylation [0323] This example demonstrates the compound of formula I reduces constitutive Akt phosphorylation in hematopoietic tumor cell lines that exhibited constitutive Akt phosphorylation. A large panel of leukemia and lymphoma cell lines was assessed for constitutive Akt phosphorylation. These cell lines represent B-lymphoma, T-lymphoma, ALL,

Malignant histiocytosis, DLBCL and AML. Cell lines that demonstrated sérum independent Akt phosphorylation were treated with the compound of formula I for 2 hours. Thereafter, cell were lysed, size-fractioned and immunoblotted with antibodies directed against phospho-Akt(Ser473).

Results are shown in Figure 8. Réduction in Akt(Ser473) was achieved for ail cell lines after exposure to compound I.

Example 9

Compound I Effective in DLBCL [0324] This example provides evidence that compound I blocks PI3K signaling and induces apoptosis in diffuse large B-cell lymphoma cells. Pl ÎOÔ is expressed in DLBCL cell lines as shown in Figure 26A. Figure 26B shows that exposure to compound I reduces pAKT levels in several DLBCL cell lines.

Example 10 ïnducement of Apoptosis in Breast Cancer Cells [0325] This example demonstrates the compound of formula I induces apoptosis in breast cancer cell lines. HS-578T, T47D, and MCF7 cells were treated with the compound of formula I or corresponding DMSO concentrations for 24 h. The percentage of apoptotic cells was determined by Annexin V-FITC/7AAD staining. Bottom left, viable cells (Annexin VF1TC/PI négative); bottom right, early apoptotic cells (Annexin V-FITC positive only); top right, mid-late apoptotic cells (Annexin V-FITC/7AAD double-positive); and top left, late apoptotic/necrotic (7AAD positive only). Percentages of cells in each quadrant are indicated except for the bottom left quadrant (viable cells). One experiment représentative of three different experiments that gave similar results is shown in Figure 10.

Example 11

Steady State Blood Levels on Day 7 in Healthy Volunteers [0326] This example provides data relating to the concentration of the compound of formula I in the blood of a healthy human subject on day 7. The concentration was monïtored over a period of 12 hours, after oral administration of 50, 100, or 200 mg BID of the compound of formula I on day 7 of the study. Figure 11 follows the plasma concentration of the drug over a xZ

period of 12 hours from administration. The maximum concentration of drug is achieved within two hours for ail doses. Administration of 50,100 or 200 mg BID of said compound results in a concentration level that exceeds the ΡΙ3Κδ EC50 concentration in basophil for at least 12 hours. [0327] In addition, single dose studies wherein 17-400 mg of the compound of formula I was administered in healthy volunteers was carried out. Concentration of the compound in the blood was measured over 24 hours from administration and results are shown in Figure 24A. At about 6 hours, the concentration of compound I in the blood for ail administered doses is at least about 100 nM. At about 12 hours, the concentration of compound I in the blood for doses 50 mg and higher is over 50 nM. The maximum concentration of compound I in the blood is achieved within 2 hours of administration.

[0328] In another experiment, the mean compound I concentration was measured on the 7th day of 50 mg BID dosing in healthy volunteers (N=6). The mean trough concentration was higher than the EC50 for ΡΙ3Κδ and the mean peak concentration was lower than the EC50 for ΡΙ3Κγ as determined in the whole blood basophil activation assay, Figure 24B. This example demonstrates the concentration range of compound I administered at 50 mg BID is at a level that is above the ED₅₀ PI3KÔ basophil activation level but lower than the minimum ED50 ΡΙ3Κγ basophil level activation level in whole blood for at least 12 hours.

[0329] Table 1, below, provides an overview of the subjects in the study, wherein either a singe dose (SD) or multiple dose (MD) of the compound of formula I is administered to a subject at varying amounts. The “n” values refer to the number of subjects in each group.

Table 1

Cohort	Regimen	Compound I	Placebo
1 (n=8)	SD	17 mg (n=6)	Placebo (n=2)
2 (n=8)	SD	50 mg (n=6)	Placebo (n=2)
3 (n=8)	SD	125 mg (n=6)	Placebo (n=2)
4 (n=8)	SD	250 mg (n=6)	Placebo (n=2)
5 (n=8)	SD	400 mg (n=6)	Placebo (n=2)
6 (n=8)	MD	50 mg BID x 7 d (n=6)	Placebo BID x 7 d (n=2)
7 (n=8)	MD	100 mg BID x 7 d (n=6)	Placebo BID x 7 d (n=2)
8 (n=8)	MD	200 mg BID x 7 d (n=6)	Placebo BID x 7 d (n=2)

Example 12

Effect on Lésions in a Patient with Mantle Cell Lymphoma [0330] This example provides data relating to the area of lésions of a patient with mantle cell lymphoma after 1 cycle of treatment (28 days) with the compound of formula I. The area of 6 lésions was measured prior to treatment and after a cycle of treatment. The response to 28 days of oral administration of 50 mg BID of the compound of formula I, results in a decrease of lésion area compared to area prior to treatment and represents a 44% decrease in tumor burden. The results are summarized in a bar graph found in Figure 12.

Example 13

Response of a Patient with CLL to Treatment [0331] This example provides data relating to the concentration of absolute lymphocyte count (ALC) in the blood of a patient with CLL after 1 cycle (28 days) of treatment with oral administration of the compound of formula I. The blood ALC concentration was measured over a period of 4 weeks after completion of one cycle of treatment. A 55% decrease in lymphocytosis and a 38% decrease in lymphadenopathy as a resuit of treatment were observed. A marked decrease in ALC concentration is observed between week 1 and week 2, Figure 13.

Example 14

Comparison of Lymphoma Patient to Healthy Volunteer [0332] This example provides data comparing the concentration of the compound of formula I in a lymphoma patient to normal healthy volunteers. On the 28^th day of oral administration of

mg BID of compound in a patient with mantle cell lymphoma, the concentration of the compound in the blood was measured over a period of 6 hours after administration. The concentration of 50 and 100 mg oral administration in normal healthy volunteers on day 7 of administration was also observed. The results are summarized in Figure 14. Thus, the compound does not build up excessively over the course of a cycle of treatment, nor does the patient become tolérant by increased metabolism over the course of the treatment cycle.

Example 15

Activity of compound 1 in various kinases [0333] This example shows the IC50 profile of compound I across classes of kinases as summarized in Table 2. While especially active on pl 1OÔ, Compound I was also active on pl 10γ and even active enough to be therapeutically useful at non-toxic doses against pl 10β, due to the demonstrated high NOAEL level of the compound; while exhibiting little activity on Class Π-V phosphoinositide kinases. Thus while being delta-selective, the compounds can exhibit sufficient activity on pl 10γ to be clinically useful, i.e._t to be effective on a cancer that relies upon pl 10γ for signaling, because a plasma level above the effective dosage for inhibition of pl 10γ can be achieved while still being sélective relative to other isoforms, particularly the alpha isoform.

Table 2

	Class I PI3Ks, IC50 (nM)	Class 11 PI3K, ICsofnM)	Class III PI3K, ICsoinM)	Class IV PI3K, IC50 (nM)	Other Phosphoinositide kinases
Compound	pl 10a	pl 100	pl I0Ô	pl 10γ	Cllbeta	hVPS34	DNA-PK	mTOR	Ρ1Ρ5Κα	ΡΙΡ5Κβ
1	435	128	1	14	>1(P	978	6,729	>1<P	>i(P	>103
NVP-BEZ- 235 Novartis	19	293	63	267	3	6	1	2	ND*	ND

InvitroGen Adapta assay *ND=not determined

Example 16

No off-target activity of Compound I in kinome-wide protein kinase screen [0334] This example demonstrates that compound ï has little or no off target activity in a kinome-wide protein kinase screen. Using Ambit KINOMEscan a genome wide screen of

over 350 protein kinases failed to detect any activity at 10 μΜ. Examples of some kinases in the screen are shown below in Table 3.

Table 3

	Examples of Relevant Kinases in Screen
ABL	FGFR1	JAK1	P38MAPK	S6K
AKT	VEGFR1	JAK2	PDGFR	SLK
ALK	FLT3	JNK1	PIM	SRC
BLK	FRK	KIT	PKA	SYK
B RAF	FYN	LCK	PKC	TAK
BTK	HCK	LYN	PLK	TIE
CDK	HER2	MAPK	RAF	TRK
CSF1R	ICK	MEK	RET	TYK
EGFR	IGF1-R	MET	ROCK	YES
EPH	ITK	MLK	ROS	ZAP70

Example 17

Selectivity of Compound 1 for pllO delta [0335] This example demonstrates that compound I is sélective for pllO delta as measured in isoform spécifie cell-based assays.

[0336] Swiss-3T3 fibroblasts and RAW-264 were seeded on a 96-well tissue culture plate and 10 allowed to reach at least 90% confluency. Cells were starved and treated with either vehicle or serial dilutions of compound 1 for 2 hrs and stimulated with PDGF or C5a respectively. Akt phosphorylation and total AKT was detected by ELISA. Purified Β-cells were treated with either vehicle or serial dilutions of compound I for 30 minutes at room température before the addition of purified goat anti-human IgM. Results are expressed as relative [³H] thymidine 15 incorporation induced by IgM crosslinking.

Table 4

PI3Ka	PI3KÔ	ΡΙ3Κγ
ECso(nM)	EC50 (nM)	ECso(nM)
Fibroblast Cell Line	Primary B Cell	Monocyte Cell Line
	BCR mediated
PDGF induced pAKT	proliferaton	C5a induced pAKT

>20,000 (n=12) (ⁿ⁼⁶)

3,894 (n=ll)

Example 18

Expression of pl 10 delta in leukemia and lymphoma cell lines [0337] This example demonstrates that PI3K pllO delta is highly expressed in a broad range of leukemia and lymphoma cell lines.

[0338] PI3K p 110δ promotes prolifération and survival in a wide range of leukemia and lymphoma cell lines. Among the cell types investigated are MCL, DLBCL, AML, ALL, and CML.

[0339] Expression of PI3K pl 10 α, β, γ and δ in a panel of lymphoma and leukemia cell lines is demonstrated in Figure 15. Proteins from 10⁶ cells were separated by SDS-PAGE and analyzed by Western blot using antibodies spécifie for the α, β, γ and δ isoforms. Purified recombinant pllO proteins were used as controls. Anti-actin antibodies were used to assess equal loading of the samples. pl 10δ was consistently expressed at a high level while other pl 10 isoforms were highly variable. PI3K pl 10δ is known to be uniformly expressed in patient AML cells as discussed by Sujobert, et al., Blood 2005 106(3), 1063-1066.

Example 19

Inhibitorv effect of Compound I on pllO delta [0340] Example 19 shows compound I inhibition of pllO delta blocks PI3K signaling in leukemia and lymphoma cell fines with constitutive pathway activation.

[0341] The PI3K pathway is frequently deregulated in leukemia and lymphoma cell lines. 48% of cell fines, or 13 out of 27, were found to hâve constitutive p-AKT. In addition, PI3K pathway activation is dépendent on pl 10δ. Compound I was found to inhibit constitutive AKT phosphorylation in 13 out of 13 cell lines.

[0342] PAGE results of Figure 9 demonstrates that constitutive AKT phosphorylation was inhibited by the presence of compound I in each of 11 cell fines, including B-cell and T-cell lymphomas. Cells were incubated for 2 hrs with 10 μΜ compound I. Cell lysâtes were run on

SDS-PAGE and transferred onto PD VF membrane and probed with appropriate antibodies.

Compound I was found to inhibit constitutive AKT phosphorylation in 11 out of 11 cell lines.

Additional cell line data for T-ALL and B-ALL cell fines is shown in Figure 27. A decrease in

Akt and S6 phosphorylation after exposure to a range concentrations of compound I (0.1 μΜ to μΜ), was quantitated by densitometry, expressed as the percent change, Figure 28.

Example 20

Compound I Inhibits Prolifération and Apoptosis in Leukemia Cell Lines [0343] Example 20 demonstrates that compound I inhibits prolifération and induces apoptosis in leukemia cell lines. Figures 16A-B show that treatment with compound I for 24 hours reduces cellular viability in a dose dépendent manner.

[0344] Prolifération assays (AlamarBlue®) on ALL cell lines grown in the presence of 10 % FBS sérum and measurements were taken at 24 hrs. Prolifération was measured in triplicate 10 wells in 96-well plates. The inhibition of PI3K signaling by compound I resulted in a block of cell cycle progression, and/or cell death. In each of six leukemia cell lines, viability was reduced by 40-50% with 10 micromolar concentrations of Compound I, Figure 16A.

[0345] Induction of apoptosis by compound I. Cells were treated with DMSO (vehicle), 1 μΜ or 10 μΜ compound I for 24 hrs. The percentage of apoptotic cells was determined by Annexin 15 V-FITC/7AAD staining. One experiment représentative of different experiments that gave similar results is shown in Figure 16B.

Example 21

Expression of pllO delta in CLL Cells [0346] This example demonstrates PI3K. pl 1 Οδ and pl 10 δ isoform expression in patient CLL 20 cells.

[0347] P13K mediated signaling pathways have been implicated in CLL. These pathways have a rôle in cell prolifération, prévention of apoptosis and cell migration. Efforts were made to détermine PI3K isoform expression in patient CLL cells.

[0348] CLL patient demographics are summarized below in Table 5.

Table 5 CLL Patient Demographics (Total (N = 24)

I) Cytogenetic abnormalities
13ql4.3	58%
llq22.3	33%
17pl3.1	20%
Trisomy 12	12%
II) Treatment History
Fludarabine refractory Unknown	29%
54%
II) IgVH Status
Mutated	33%
Unmutated	33%
Unknown	33%

[0349] The PAGE images of Figure 17A-D compare the expression of pl 10a, pl ΙΟδ, pl ΙΟβ, and plΙΟγ in CLL cells of patients A-E. pl ΙΟδ and pl ΙΟγ is expressed in each patient compared to the other PI3K isoforms.

Example 22

Compound I induces cleavage of caspase 3 and PARP [0350] This example demonstrates that compound I induced the cleavage of caspase 3 and

PAJRP. Figures 18A-B show results of caspase 3 and PARP (Poly(ADP) Ribose Polymerase) cleavage in the presence of 1,10 μΜ of compound I or 25 μΜ of LY294002.

[0351] Further experiments provide evidence of compound I inducing caspase 2 and PARP cleavage. Cells were cultured with compound I or vehicle alone for 24 hrs. Thereafter, cells were lysed and sized-fractionated and immunoblotted with antibody directed against FLIP, Figure 29. Additionally, whole cell lysâtes were added to MDS (Meso Scale Diagnostics) multispot 96-well 4 spot plates coated with Total caspase-3, cleaved caspase-3, cleaved PARP, and BSA. Proteins were detected with antibodies labeled with SULFO-TAG reagent and quantified. A dose dépendent response in the cleavage of caspase 3 and PARP was achieved upon exposure 20 to 5 or 10 μΜ of compound I.

Example 23

Compound I Blocks PI3K Signaling [0352] This example demonstrates that compound I blocks PI3K signaling in patient AML cells. PI3K8 is implicated in signaling in AML patient cells. Figure 21 shows the results of

Phospho-Akt production in the absence or presence of 0.1,1.0,10 μΜ of Compound I. This provides evidence that compound I reduces phopsho-Akt production in patient AML cells.

Example 24

Measurement of PI3K signaling in basophils founding whole-blood [0353] This example demonstrates a whole-blood assay for measurement of PI3K signaling in basophils using flow cytometry by the induction of CD63 surface expression.

[0354] Inhibition of PI3K signaling in basophils permits compound I to be a useful pharmacodynamie marker. PI3K signaling is monitored by CD63 surface expression. In particular, pl 108 médiates FCeRl signaling and pl 10γ médiates fMLP receptor signaling. The flow cytometry analysis of PI3K mediated CD63 expression on basophils comprises the following sequential steps:

1. Collect peripheral blood

2. Basophil stimulation (fMLP or Anti-FCeRl Mab)

3. Label basophils (Anti-CCR3-FITC and Anti-CD63-PE)

4. Lyse and fix cells

5. Analysis by flow cytometry [0355] Figure 22A-C compares the results of A) no stimulation, B) stimulation with AntiFCeRl, or C) stimulation with fMLP.

[03561 Figure 23 shows that Compound I is especially active where pl 108 mediated signaling is most important, but is also relatively active where pl 10γ is utilized: it achieved 50% réduction in SD63 expression at « 1 μΜ for the pl 108 test, and ca. 10 μΜ for the pl 10γ test. Basophil activation was measured in human whole blood using the Flow2 CAST® kit. Whole blood samples were treated with either vehicle or serial dilutions of compound I prior to activation of basophils either with anti-FceRI mAb or fMLP. Cells were stained with the combination of anti-human CD63-FITC and anti-human CCR3-PE mAbs. The percent CD63

positive cells within the gated basophil population were determined in different treatment groups and normalized to the vehicle control.

Ex ample 25

Compound I Reduces Lymphadenopathy in CLL Patient [0357] This example provides evidence of the réduction in size of a bulky lymphadenopathy in a CLL patient with a del[17p]. A patient with del(17p) had an axillary lymphadenopathy, which was imaged by computed tomography (CT) to provide a baseline measurement of 5.9 cm x 4.1 cm, Figure 40A. After one cycle of treatment with compound I, the lymphadenopathy was reduced to a dimension of 3.8 x 1.8 cm, Figure 40B. A cycle treatment was 28 days of continuous oral dosing at either 200 mg BID or 350 mg BID of compound I.

Example 26

Limited Effect of Compound I on Glucose and Insulin Levels of a Subject [0358] This example demonstrates that treatment with compound I has little or no effect on glucose and insulin levels. Compound I was administered at 50-200 mg amounts BID to a subject over a period of up to 10 days. Blood glucose and insulin concentrations were measured over time and compared to placebo results as shown in Figures 25A-B.

[0359] Blood glucose concentration remained steady after 10 days of treatment with even the highest dosage amount of compound L Insulin levels remained within the normal range after 7 days of treatment with compound I. This provides evidence that compound I has little or no effect on glucose and insulin levels.

Example 27

Materials and Methods [0360] This example provides information on materials and methods of carrying out the experiments described in Examples 28-35 which relate to the use of compound I in the treatment of multiple myeloma.

Materials [0361] pl 10δ inhibitor compound I and compound II were provided by Calistoga

Pharmaceuticals, (Seattle, WA). The sample of compound I and II used was over 95% the

S enantiomer. Compound I was dissolved in Dimethyl sulphoxide at 10 mM and stored at -20°C for in vitro study. Compound II was dissolved in 1% carboxyl methylcellulose (CMC)/0.5% Tween 80 and stored at 4°C for In vivo study. Recombinant human PI 10α, β, γ, and Ô were reconstituted with stérile phosphate-buffered saline (PBS) containing 0.1% BSA. bortezomib was provided by Millennium Pharmaceuticals (Cambridge, MA). 3-Methyladenine was purchased from Sigma-Aldrich (St. Louis, MO).

Cell culture [0362] Dex-sensitive (MM.1S) and résistant (MM.1R) human MM cell lines were kindly provided by Dr. Steven Rosen (Northwestern University, Chicago, IL). H929, RPMI8226, and U266 human MM cell lines were obtained from American Type Culture Collection (Manassas, VA). Melphalan- résistant RPMI-LR5 and Doxorubicin (Dox)-resistant RPMI-Dox40 cell lines were kindly provided by Dr. William Dalton (Lee Moffitt Cancer Center, Tampa, FL). OPM1 plasma cell leukemia cells were provided by Dr. Edward Thompson (University of Texas Medical Branch, Galveston). IL-6-dependent human MM cell line INA-6 was provided by Dr. Renate Burger (University of Kiel, Kiel, Germany). LB human MM cell line was established in the laboratory. Phenotypic analysis revealed no cytogenetic abnormalities. Phenotypic analysis is shown in table 6. CD expression profile of LB cell line, defined by flowcytometric analysis.

Table 6

LB expression

CD marker	% expression
CD3	5.5 %
CD19	61.7%
CD20	97.2 %
CD38	54.1 %
CD40	96.8 %
CD49e	5.9 %
CD70	98.0 %
CD138	96.3 %

[0363] Ail MM cell fines were cultured in RPMI1640 medium. Bone marrow stromal cells (BMSCs) were cultured in Dulbecco’s modification of Eagle’s medium (DMEM) (Sigma) containing 15% fêtai bovine sérum, 2 mM L-glutamine (Life Technologies),100 U/mL

penicillin, and 100 pg/mL streptomycin (Life Technologies). Blood samples collected from healthy volunteers were processed by Ficoll-Paque™ gradient to obtain peripheral blood mononuclear cells (PBMNCs). Patient MM and BM cells were obtained from BM samples after informed consent was obtained per the Déclaration of Helsinki and approval by the Institutional Review Board of the Dana-Farber Cancer Institute (Boston, MA). BM mononuclear cells were separated using Ficoll-Paque™ density sédimentation, and plasma cells were purified (>95% CD138+) by positive sélection with anti-CD138 magnetic activated cell séparation micro beads (Miltenyi Biotec, Auburn, CA). Tumor cells were also purified from the BM of MM patients using the RosetteSep négative sélection system (StemCell Technologies, Vancouver, BC, Canada).

Growth inhibition assay [0364] The growth inhibitory effect of compound I on growth of MM cell fines, PBMCs, and BMSCs was assessed by measuring 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetra-sodium bromide (MTT; Chemicon International, Temecula, CA) dye absorbance.

Effect of compound I on paracrine MM cell growth in the BM [0365] MM cells (2 x 104 cells/well) were cultured for 48 h in BMSC coated 96-well plates (Costar, Cambridge, MA), in the presence or absence of drug. DNA synthesis was measured by [3H]-thymidine (Perkin-Elmer, Boston, MA) uptake, with [3H]-thymidine (0.5 μ Ci/well) added during the last 8 h of 48 h cultures. Ail experiments were performed in quadruplicate.

Transient knockdown of Pl 105 expression [0366] INA-6 cells and LB cells were transiently transfected with siRNA ON-TARGET plus SMART pool Pl 10δ or nonspecific control duplex (Dharmacon Lafayette,Co) using Cell Line Nucleofector Kit V (Amaxa Biosystems Gaitherburg,MD).

Immunofluorescence [0367] Viable MM cells (2.5 X 104) were pelleted on glass slides by centrifugation at 500 rpm for 5 minutes using a cytospin system (Thermo Shandon, Pittsburgh, PA). Cells were fixed in cold absolute acetone and methanoi for 10 min. Foilowing fixation, cells were washed in phosphate-buffered saline (PBS) and then blocked for 60 min with 5% FBS in PBS. Slides were then incubated with anti-CD138 antibody (Santa Cruz Biotechnology, Santa Cruz, CA) at 4’C for 24 h, washed in PBS, incubated with goat anti-mouse IgG for 1 h at 4°C, and analyzed using Nikon E800 fluorescence microscopy.

Détection and quantification of Acidic Vesicular Organelles f AVO) with acridine orange staining.

[0368] Autophagy was characterized by séquestration of cytoplasmic proteins and development of AVOs. To detect and quantify AVOs in compound I or 3MA -treated cells, vital 5 staining was performed for 15 min with acridine orange at a final concentration of 1 pg/ml.

Samples were examined under a fluorescence microscope.

Angiogenesis assay [0369] The anti-angiogenic activity of compound I was determined using an in vitro Angiogenesis Assay Kit (Chemicon, Temecula, CA). HUVEC and endothélial growth media 10 were obtained from Lonza (Walkersville, MD, USA). HUVEC were cultured with compound I on polymerized matrix gel at 37°C. After 8 h, tube formation was evaluated using Leika DM IL microscopy (Leica Microsystems, Wetzlar, Germany) and analyzed with IM50 software (Leica Microsystems Imaging Solutions, Cambridge, UK). HUVEC cell migration and rearrangement was visualized, and the number of branching points counted.

Western blotting [0370] MM cells were cultured with or without compound I; harvested; washed; and lysed using radïoimmuno précipitation assay (RIPA) buffer, 2 mM Na3VO₄, 5m M NaF, 1 mM phenylmethylsulfonyl fluoride (5 mg/ml). Whole-cell lysâtes were subjected to sodium dodecyl suIfate-polyacrylamide gel electrophoresis (SDS-PAGE) séparation, transferred to Pure

Nitrocellulose membranes (Bio-Rad Laboratories, Hercules, CA), and immunoblotted with antiAKT, phospho(p)-AKT (Ser473, Thr 308), ERK1/2 , P-ERK1/2, P-PDK1, STAT, P-STAT, P-FKRHL, P-70S6K, LC3, and PI3K/pl 10 a Abs (Cell Signaling Danvers, MA); anti-pl 1 Οβ, PI3K/pl 10Ô, Glyceraldehyde 3-phosphate dehydrogenase (GAPDH), α-tubulin, and actin Abs (Santa Cruz Biotechnology, CA); and anti-pl 10 γ Ab (Alexis, San Diego, CA): and antî-LC3 Ab (Abgent, San Diego, CA).

ELISA [0371] Cytokine sécrétion by human BMSCs cocultured with MM cells was assessed by

ELISA. BMSCs were cultured in 96-well plates with varying concentrations of compound I, with or without INA-6 cells. After 48 h, supernatants were harvested and stored at -80°C.

Cytokines were measured using Duo set ELISA Development Kits (R&D Systems, Minneapolis,

MN). Ail measurements were carried out in triplicate.

Human cytokine array [0372] The cytokine levels in culture supernatants were assessed using Proteome Profiler Antibody Arrays Panel A (R&D Systems, Minneapolis, MN), Supernatants from co-cultures with BMSCs were incubated for 4 hours with membranes arrayed with Abs against 37 cytokines, according to manufacturer’s instructions.

Murine xenograft models of human MM [0373] CB17 SCID mice (48-54 days old) were purchased from Charles River Laboratories (Wilmington, MA). Ail animal studîes were conducted according to protocols approved by the Animal Ethics Committee of the Dana-Farber Cancer Institute. Mice were înoculated subcutaneously in the right fiank with 3X106 LB cells in 100 pL RPMI-1640. When tumors were palpable, mice were assigned into the treatment groups receiving 10 mg/kg or 30 mg/kg gavages twice daily; and 7 mice in the control group receiving vehicle alone. Caliper measurements of the longest perpendicular tumor diameters were performed every altemate day to estimate the tumor volume using the following formula representing the 3D volume of an ellipse: 4/3 X (width/ 2)2 X (length/2). Animais were sacrificed when tumors reached 2 cm or the mice appeared moribund. Survival was evaluated from the first day of treatment until death. Tumor growth was evaluated using caliper measurements from the first day of treatment until day of first sacrifice, which was day 12 for the control group and days 17 and 19 for the treatment groups. The images were captured with a canon IXY digital 700 caméra. Ex vivo analysis of tumor images was captured with a LEICA DM IL microscope and LEICA DFC300 FX caméra at 40u/0.60 (Leica, Heidelberg, Germany).

[0374] Human fêtai bone grafts were implanted into CB17 SCID-mice (SCID-hu). Four weeks following bone implantation, 2.5 X 106 INA-6 cells were injected directly into the human BM cavity in the graft in a final volume of 100 μΐ of RPMI-1640 medium. An increase in the levels of soluble human IL-6 receptor (shuIL-6R) from INA-6 cells was used as an indicator of MM cell growth and burden of disease in SCID-hu mice. Mice developed measurable sérum shuIL-6R approximately 4 weeks following INA-6 cell injection, and then received either 10 or 30mg/kg drug or vehicle alone daily for 7 weeks. Blood samples were collected and assessed for shuIL-6R levels using an enzyme-linked immunosorbent assay (ELISA, R&D Systems. Minneapolis MN),

Statistical analysis [0375] Statistical sîgnificance was determined by Dunn’s multiple comparison tests. The minimal level of significance was p<0.05. Survival was assessed using Kaplan-Meier curves and log-rank analysis. The combined effect of compound I and bortezomib was analyzed by isobologram analysis using the CalcuSyn software program (Biosoft, Ferguson, MO); a combination index (CI) <0.7 indicates a synergistic effect.

Example 28

Expression of pllO delta in MM cells [0376] This example demonstrates that pllO delta is highly expressed in patient MM cells. To assess PI3K/p 110 expression, Abs was used against recombinant human PI3K/pl ΙΟα, β, γ, and δ proteins with spécifie immunoreactivity against these isoforms. The expression of pl lΟδ in 11 MM cell lines (MM.1S, OPM1, OPM2, RPMI8226, DOX40, LR5, MM.1R, U266, INA-6, H929, and LB), as well as 24 patient MM samples were evaluated and immunoblots shown in Figure 30A and 30B. Figure 30A shows expression of ρ110-α,-β, -γ, and -δ in MM cell lines detected by immunoblotting using spécifie antibodies. Anti-a-Tubulin MAb served as a loading control. pl 106 in patient MM cells was detected by immunoblotting using anti-Pl 10δ Ab (Figure 30B).

[0377] Anti-GAPDH MAb served as a loading control. INA-6 and LB cells strongly expressed pl 10δ, whereas MM.1S, OPM1, MM.1R, Dox40, U266 or H929 lacked pl 10δ expression (Figure 30A).

[0378] pl 10δ expression in MM. 1S and LB cells was confîrmed by immunofluorescence analysis (Figure 30C). Human recombinant PllO-α,-β,-γ,-δ proteins in SDS sample buffer were heated for 3 min prior to loading on gel. (10-20 pg per lane.) Recombinant human PllO-α,-β,-γ,-δ proteins were detected by Immunoblot analysis. Levels of Pl 10 6 were measured in MM1S and LB cells using Pl 10 Ô spécifie FITC conjugated secondary antibodies. Pl 1 Οδ stained green, and nucleic acids (DAPI) stained blue, [0379] Western blotting revealed no corrélation of between pl 10δ expression and expression of the other isoforms (α, β and γ). Important y, ail patient MM cells also expressed pl 10δ, (Figure 30B).

Example 29

Cytotoxicity of compound I on MM cells [0380] This example demonstrates that compound I has sélective cytotoxicity against cells with pl 1OÔ. Specifically, compound I potently induced cytotoxicity in pl 10 delta positive MM cells as well as in primary patient MM cells without cytotoxicity in peripheral blood mononuclear cells from healthy donors, suggesting a favorable therapeutic index.

[0381] The growth inhibitory effect of pl 1 Οδ knockdown in MM cells was evaluated. LB and INA-6 cells were transfected with Pl 1 Οδ sîRNA (Si) or control siRNA (Mock). After 24 h, expression ofPl 10 δ wasdetermined by western blot analysis, see Figure 31 A. INA-6 cells were transfected with pl IOô siRNA or control siRNA, and then cultured for 72 hours. Cell growth was assessed by MTT assay, see Figure 31 B. Data indicates mean ± SD of triplicate cultures, expressed as fold of control. Transfection with pl 105 siRNA, but not mock siRNA, down-regulated pl 1 Oô and inhibited MM cell growth at 72 h (Figure 31A and 31B). The growth inhibitory effect of pl 105 spécifie small molécule inhibitor compound I in MM cell lines,

PBMCs, and patient MM cells was evaluated.

[0382] Compound I induced cytotoxicity against LB and INA-6 MM cells (pl ΙΟδ-positive) in a dose- and time- dépendent fashion; in contrast, minimal cytotoxicity was noted in pl 1 Οδnegative cell lines (Figure 31C). The legend for Figure 31C: LB (□), INA-6 (Δ), RPMI 8226(o), OPM2 (Ο), H929 ( · ), U266 (♦),RPMI- LR5 (À) and OPM1 () MM cells were cultured with or without compound I for 48h.

[0383] Importantly, compound I also induced cytotoxicity against patient MM cells (Figure 31D), without cytotoxicity in PBMCs from 4 healthy volunteers at concentrations up to 20 μΜ (Figure 31E). Patients MM cells isolated from BM by négative sélection were cultured with compound I for 48h. Peripheral blood mononuclear cells isolated from healthy donors were cultured with compound I for 72 h. Data represent mean ± SD viability, assessed by MTT assay of triplicate cultures, expressed as percentage of untreated controls. These results strongly suggest that sensitivity to compound I is associated with Pl 10Ô expression, and suggest a favorable therapeutic window.

[0384] To détermine whether the cytotoxicity induced by compound I is via apoptosis, the cleavage of caspases and PARP by western blot analysis was examined. INA-6 cells were cultured with compound I (0-5μΜ) for 120 h. Total cell lysâtes were subjected to

immunoblotting using anti-caspase-3, -8, -9, PARP, and α-tubulin Abs. FL indicates full-length protein, and CL indicates cleaved protein. Significantly increased cleavage of caspase-8, caspase-9, caspase-3, and PARP was observed in INA-6 MM cells treated with compound I for

120 h (Figure 31F). These results indicate that cytotoxicity triggered by compound I is mediated, at least in part, via caspase-dependent (both intrinsic and extrinsic) apoptosis.

Example 30

Inhibition of AKT and ERK phosphorylation by compound I [0385] This example demonstrates the Inhibition of AKT and ERK phosphorylation by compound I.

[0386] An important downstream effector of PI3K is the serine/ threonine protein kinase AKT, which is activated by phosphorylation of Thr308 in the activation loop of the kinase domain and Ser473 in the C-terminal tail. Phosphorylation of both sites requires an interaction between the N-terminal pleckstrin homology domain of AKT and membrane phosphoinositide generated by PI3K. It was shown that compound I inhibits both domains, suggesting that P110Ô is the prédominant isoform responsible for PI3K signaling in MM cell lines.

[0387] Inhibition of AKT and ERK pathways in INA-6 cells by compound I was examined. INA-6 cells were cultured with Compound I or LY294002 for 12 h, Figure 32A. Actin Ab was used as a loading control. INA-6 and MM.1S cells were cultured with Compound I (0,0.25,1.0,5.0 μΜ) for 6 hours, Figure 32B. LB and INA-6 cells were cultured with compound I for 0-6 hours, Figure 32C. Whole cell lysâtes were subjected to immunoblotting using AKT, P-AKT (Ser473 and Thr308), ERK1/2, P-ERK1/2, P-PDK1, and P-FKRHL antibodies. atubulin ïs used as a loading control.

[0388] Compound I significantly blocked phosphorylation of AKT and ERK1/2 in pl 10Ô positive INA-6 cells (Figure 32A), but did not affect phosphorylation of AKT or ERK in MM.1S cells with low expression of Pl 10δ (Figure 32B). Compound I also significantly inhibited phosphorylation of upstream PDK-1 and downstream FKHRL in INA-6 and LB MM cells in a time- and dose- dépendent fashion (Figure 32C), further confirming inhibition of a both PI3K/AKT and ERK pathways in these cells.

Example 31

Compound I induces AVO development and autophagy [0389] This example demonstrates the ability of compound I to trigger both apoptosis and autophagy.

[0390] AKT régulâtes autophagy, thus investigation of compound I in inducing autophagy in

LB and INA-6 MM cells was carried out.

[0391] INA-6 and LB MM cells were treated with 5 pM Compound I for 6h. Compound I treatment induced LC3 accumulation in LB and INA-6 cells, evidenced by fluorescence microscopy or transmission électron microscopy. Autophagosome formation was defined by the 10 accumulation of LC3; arrows indicate autophagosomes, Figure 33A.

[0392] INA-6 cells were treated with 5 pM Compound I or sérum starvation for 6h, stained with 1 pg/mL acridine orange for 15 min, and analyzed by fluorescence microscopy, Figure 33B.

[0393] LC3 and beclin-1 protein levels were determined by western blotting using LC3 and beclin-1 antibodies of lysâtes from INA-6 cells treated with Compound I, with or without 3-MA, Figure 33C. GAPDH served as a loading control.

[0394] Immunofluorescence analysis showed markedly increased LC 3 staining in INA-6 and LB cells triggered by compound I (5pM, 6 h) treatment (Figure 33A). Electron microscopie analysis also showed increased autophagie vacuoles (arrows) in MM cells treated with compound I. Since autophagy is characterized as acidic vesicular organelle (AVO) development, acridine orange staining was carried out. As shown in Figure 33B, vital staining with acridine orange revealed development of AVOs in compound I- treated LB and INA-6 cells. Moreover, markedly increased LC3-II and Beclinl protein were detected in INA-6 MM cells after 6 h treatment with compound I, which was blocked by 3-MA autophagie inhibitor (Figure 33C).

[0395] No cytotoxicity in INA-6 and LB cells was induced by 3-MA at concentrations up to 100 pM, Figure 33D. PI 10 Ô positive LB cells ( ♦ ) were treated with 3-MA (0-100 pM) for 24h. Data represent means (±SD) of triplicate cultures.

[0396] These results indicate that compound I induces development of AVOs and autophagy at earlier time points than induction of caspase/PARP cleavage.

[0397] Autophagy dégradés cellular components, recycles cellular constituents, and responds to various cellular stress. In this example, LC3-II, a hallmark of autophagy, is induced by compound I treatment in pl 10 Ô positive MM cell lines. Importantly, compound I treatment resulted in a marked increase in autophagy, evidenced by the presence of autophagie vacuoles in the cytoplasm, formation of AVOs, membrane association of microtubule-associated protein I of LC3 with autophagosomes, and a marked induction of LC3-II protein. Electron microscopie analysis confirmed that compound I induced autophagosomes. LC3-II was expressed through LC3-I conversion. Conversely, autophagy induced by compound I was suppressed by 3-MA, a spécifie inhibitor of autophagy. These studies suggest that early cytotoxic effects of compound I are associated with autophagy.

Example 32

Compound I inhibits cell growth in the presence of BMSC [0398] This example demonstrates the ability of compound I to inhibit paracrine MM cell growth with BMSCs.

[0399] Since IL-6 and IGF-1 induces growth and anti-apoptosis in MM cells, compound I was examined in overcoming the effects of these cytokines in INA-6 and LB MM cells. LB and INA-6 cells were cultured for 48h with control media ( ); or with compound 1 at 5.0 μΜ ( ) or 10 μΜ ( □), in the presence or absence of IL-6 (1 and 10 ng/ml), Figure 34A, or IGF-1 (10 and 100 ng/mL), Figure 34B. DNA synthesis was determined by measuring [3H]-thymidine incorporation during the last 8h of 72h cultures. Data represent means (±SD) of triplicate cultures. Neither IL-6 nor IGF-1 protected against the growth inhibition induced by compound I (Figure 34A and 34B).

[0400] The BM microenvironment confers prolifération and drug-resistance in MM, thus MM cell growth inhibitory effect of compound I in the presence of BMSCs was examined.

[0401] LB and INA-6 MM cells were cultured for 48h with control media ( D ), and with

2.5 μΜ (®), 5 μΜ (®), and 10 μΜ ( ) of Compound I, in the presence or absence of BMSCs,

Figure 34C. DNA synthesis was determined by [3H]-thymidine incorporation. Data represent means (±SD) of triplicate cultures.

[0402] IL-6 in culture supernatants from BMSCs treated with compound I (0-2.5 μΜ) was measured by ELISA, Figure 34D. Error bars indicate SD (±).

[0403] BMSCs were cultured with 1.0 μΜ compound I or control media for 48h; cytokines in culture supernatants were detected using cytokine arrays, Figure 34E.

[0404] INA-6 cells cultured with or without BMSCs were treated with compound for 48h. Total cell lysâtes were subjected to immunoblotting using indicated antibodies, Figure 34F.

Actin was used as a loading control.

[0405] BMSCs from 2 different patients (□, O) were cultured with compound I (0-20pM) for 48h. Cell viability was assessed by MTT assay, Figure 34G. Values represent mean ± SD of triplicate cultures.

[0406] Importantly, compound I inhibited growth and cytokine sécrétion (Figure 34C-E), as well as phosphorylation of AKT and ERK (Figure 34F), induced by BMSCs. In contrast, no significant growth inhibition in BMSCs was noted (Figure 34G). These results indicate that compound I blocks paracrine MM cell growth in the context of the BM microenvironment.

Example 33

Compound I inhibits angiogenic HuVEC tubule formation [0407] This example demonstrates the ability of Compound I to inhibit HuVEC tubule formation. The rôle of PI3K, specifically pllO isoform, in angîogenesis was investigated. Endothélial cells are an essential regulator of angîogenesis for tumor growth. Both Akt and ERK pathways are associated with endothélial cell growth and régulation of angîogenesis; and importantly, endothélial cells express pl 10δ. This example also demonstrates that compound I blocks in vitro capillary-like tube formation, associated with down régulation of Akt phosphorylation.

[0408] The effect of Pl 10 δ inhibition on angîogenesis was investigated. HuVECs were treated with 0, 1.0, or 10 μΜ of compound I for 8 h, and tube formation by endothélial cells was evaluated (Figure 35A). HuVEC cells were plated on Matrigel-coated surfaces and allowed to form tabules for 8 h, in the presence or absence of Compound I. Endothélial cell tube formation was measured by microscopie analysis, Figure 35B. *P<0.005.

[0409] HuVECs were cultured with Compound I (0-20μΜ) 48h, and viability was assessed by

MTT assay, Figure 35C. Data shown are mean ± SE of triplicate wells from a représentative experiment. Thus, compound I inhibited capillary-like tube formation in a dose-dependent fashion (p<0.05) (Figure 35B), without associated cytotoxicity (Figure 35C).

[0410] Phosphorylation and expression of AKT and ERK1/2 was markedly down regulated in HuVEC cells by compound I treatment, HuVECs were cultured with compound I (0-200μΜ) for 8h, and cell lysâtes were analyzed by immunoblotting using the indicated antibodies, Figure 35D. Actin was used as a loading control.

[0411] These findings suggest that compound I can inhibit angiogenesis, associated with down régulation of AKT and ERK activity.

Example 34

Compound II inhibits MM cell growth In vivo [0412] This example demonstrates the ability of compound II to inhibit human MM cell 10 growth In vivo.

[0413] The In vivo efficacy of Pl 10δ inhibitor was evaluated in a xenograft model in which SCID mice are injected subcutaneously with human MM cells.

[0414] Mice injected with 5x 10⁶ LB cells were treated orally twice a day with control vehicle ( · ), and compound II lOmg/kg (□) or 30mg/kg ( o ). Mean tumor volume was calculated as in 15 Materials and Methods, Figure 36A. Error bars represent SD (±), [0415] Représentative whole-body images from a mouse treated for 12 d with control vehicle (top panel) or Compound II (30mg/kg) (bottom panel), Figure 36B.

[0416] Tumors harvested from Compound II (30mg/kg) treated mouse ( right panel ) and control mouse (left panel) were subjected to immuno-histochemistric analysis using CD31 and 20 P-AKT Abs. CD31 and P-AKT positive cells are dark brown, Figure 36D.

[0417] Mice were treated with Compound II lOmg/kg ( “ - ), 30mg/kg („.)or Control vehicle ( - ). Survival was evaluated from the first day of treatment until sacrifice using Kaplan-Meier curves, Figure 36C.

[0418] Tumor tissues were harvested from mice treated with control vehicle or Compound II 25 (30mg/kg). Protein levels of phosphorylated of PDK-1 and AKT (Ser473) were determined by western blotting of cell lysâtes, Figure 36E. Actin was used as a loading control.

[0419] Growth of INA-6 cells engrafted in human bone chips in SCID mice was monitored by serial sérum measurements of shuIL-6R. Mice were treated with Compound II lOmg/kg ( □ ), 30mg/kg ( Δ ) or control vehicle( · ), and shuIL-6R levels were determined weekly by

ELISA, Figure 36F. Error bars indicate SD (±).

[0420] Compound II (pllO Ô inhibitor) significantly reduced MM tumor growth in the treatment group (n=7) compared with control mice (n=7). Comparison of tumor volumes showed statistically significant différences between control versus treatment groups (vs 10 mg/kg, P<0.05; vs 30 mg/kg, P<0.01) (Figure 36A). Marked decrease in tumor growth in treated versus in control mice was observed at day 12. (Figure 36B) Kaplan-Meier curves and log-rank analysis showed a mean Overall Survival (OS) of 15 days (95% confidence interval, 12-17 days) in control mice versus 23 days (95% CI, 15-34 days) and 32 days (95% CI, 27-49 days) in the 10 mg/kg and 30mg/kg compound II treated groups, respectively. Statistically significant prolongation in mean OS compared with control mice was also observed in treatment groups (vs 10 mg/kg, P=0.086; vs 30 mg/kg, P=0.056) (Figure 36C). Importantly, treatment with either the vehicle alone or compound II did not affect body weight. In addition, immunohistochemical (Figure 36D) and immunoblot (Figure 36E) analysis confirmed that compound II treatment (30mg/kg) significantly inhibited p-Akt and p-PDK-1, as well as significantly decreased CD31 positive cells and microvessel density (p<0.01) (Figure 36D). This suggests that compound II can inhibit angiogenesis In vivo via suppression of the Akt pathway.

[0421] In order to examine the activity of compound II on MM cell growth in the context of the human BM microenvironment In vivo, a SCID-hu model was used in which IL-6 dépendent INA-6 cells are directly injected into a human bone chip implanted subcutaneously in SCIDmice. This model récapitulâtes the human BM microenvironment with human IL- 6/ BMSCdependent growth of INA-6 human MM cells. These SCID-hu mice were treated with compound II or vehicle alone daily for 4 weeks, and sérum shuIL-6R monitored as a marker tumor burden. As shown in Figure 36F, compound II treatment significantly inhibited tumor growth compared with vehicle control. Significant tumor growth inhibition in this model was observed, evidenced by decreased sérum shuIL-6R levels released by INA-6 cells, confirming that pl 10δ inhibition blocks the MM growth promoting activity of the BM microenvironment In vivo. Taken together, these data demonstrate that inhibition of pl 10δ by compound II significantly inhibits MM growth In vivo and prolongs survival.

Example 35

Compound I in combination with bortezomib exhibits synergistic cytotoxicity [0422] This example demonstrates the effect of Compound I in combination with bortezomib to médiate synergistic MM cytotoxicity.

[0423] The effects of combining compound I with bortezomib in inducing synergistic MM cytotoxicity was investîgated. LB and INA-6 MM cells were cultured with medium () and with compound I, 1.25 μΜ (). 2.5μΜ (H), or 5.0μΜ (□), in the presence or absence of bortezomib (0-5nM). Cytotoxicity was assessed by MTT assay; data represent the mean ±SD of quadruplicate-cultures, Figure 37A.

[0424] INA-6 cells were treated with Compound I (5μΜ) and/or bortezomib (5nM) for 6h. Phosphorylation of AKT was determined by western blotting of cell lysâtes using phospho-AKT (ser473) antibody, Figure 37B. Actin served as a loading control.

[0425] Compound I enhances cytotoxicity of bortezomib. Increasing concentrations of compound I (1.5-5.0μΜ) added to bortezomib (2.5, 5.0 nM) triggered synergistic cytotoxicity in LB and INA-6 MM cells (Figure37A and Table 7). Importantly, induction of phospho-Akt by bortezomib treatment was inhibited in the presence of compound I (Figure 37B).

Table 7

Combination index (Cl)

Bortezomib	Compound 1	Fa	Cl
(nM|	(μΜ)
2.5	1.25	0.39	0.57
B 25	2.5	0.52	0.56
2Ég	5	0.57	0.67
5	1.25	0.42	0.88
5	2.5	0.60	0.25
5	5	0.67	0.22
2.5	1.25	0.49	0.31
2.5	2.5	0.56	0.48
IA-6 2,5	5	0.69	0.54
5	1.25	0.56	0.73
5	2.5	0.66	0.42
5	5	0.75	0.31

Example 36

Compound I effective in Follicular Lymphoma cell lines [0426] This example provides evidence that compound I blocks PI3K signaling and induces apoptosis in follicular lymphoma cells. PI 10δ is expressed in FL cell lines as shown in Figure

38A. Certain cell lines show réduction in the production of pAkt, Akt, pS6 and S6 when the cell is exposed to compound I, Figure 38B. Cleavage of PARP and Caspase-3 is observed after exposure to compound I in a dose dépendent fashion after 24 hours at 0.1 μΜ and 0.5 μΜ,

Figure 38C.

Example 37

Compound I effective in primary MCL cells [0427] This example demonstrates that compound I is effective against MCL. Compound I was found to block constitutive PI3K signaling in primary MCL cells of two patients in a dose dépendent manner when exposed to 0.1 μΜ or 1 μΜ of compound I, Figure 39A. Compound I is also observed to inhibit survival factor and chemokine signaling in MCL cell lines. Figure 39B shows a significant réduction of pAkt in MCL lines exposed to different survival factors in the presence of compound I.

Example 38

Effect of Compound in Combination with Rituximab and/or Bendamustine in Patients with Relapsed or Refractory B-cell Malignancies [0428] This example demonstrates the safety and activity of the compound of formula I in combination with rituximab and/or bendamustine in patients with relapsed or refractory B-cell malignancies.

[0429] At data cutoff, 12 patients were enrolled in the study, including 6 with NHL and 6 with CLL. Patients included: males/females n=8 (67%)/4 (33%) with médian âge of 65 (Range: 55-80) years, and relapsed/refractory disease n=8 (67%)/4 (33%). The médian number of prior thérapies was 3 (Range: 1-11). Ail patients received the compound of formula 1100 mg BID; 6 patients received rituximab and 6 received bendamustine. One patient with NHL had a dose réduction of bendamustine due to hiccups and 1 patient with NHL had a dose réduction of the compound of formula I due to increased ALT/AST; ail other patients received the full-dose regimen with acceptable tolerability. Clinical response assessments were available for 6 patients who had completed 2 cycles of combination treatment and the results are shown in Table 8 below.

Table 8.

Regimen

Disease

The compound of formula I + bendamustine

CLL

No. of Prior Thérapies 3

Response

Partial response

The compound of formula I + bendamustine	NHL	2	Complété response
The compound of formula I + bendamustine	NHL	3	Partial response
The compound of formula I + rituximab	NHL	4	Partial response
The compound of formula I + rituximab	CLL	11	Partial response
The compound of formula I + rituximab	CLL	3	Progressive disease

[0430] Thus, administration of the compound of formula I in combination with rituximab or bendamustine shows acceptable safety and promising clinical activity in patients with relapsed or refractory B-cell malignancies.

Example 39

Effect of Compound in Combination with Rituximab and/or Bendamustine in Patients with

Relapsed or Refractory B-cell Malignancies [0431] This example demonstrates the safety and activity of the compound of formula I in combination with rituximab and/or bendamustine in patients with relapsed or refractory B-cell indolent NHL and CLL.

[0432] 20 patients were enrolled, including 12 with iNHL and 8 with CLL. Patient characteristics are summarized in Figure 42. Ail patients received the compound of formula I, either 100 mg orally twice per day (BID) or 150 mg orally twice per day (BID) for as long as the patient was benefitting. Patients also received either rituximab 375 mg/m² adminîstered weekly for 8 weeks, starting on Day 1 of Cycle 1, or bendamustine 90 mg/m² adminîstered on Days 1 and 2 of each cycle for 6 cycles. Tumor response was evaluated according to standard criteria.

[0433] As shown in Figure 43, Grade a3 adverse events included largely comprised background events resulting from pre-existing disease- or treatment-related conditions or from intercurrent illness. For patients receiving bendamustine and Compound I, Grade 2:3 adverse events included B-induced myelosuppression. For patients receiving rituximab and Compound I, Grade a3 adverse events were infrequent and not clearly related to the compound of formula I.

iNHL-specific transient ALT/AST élévations were observed; these events resolved upon drug interruption and were successfully managed with reinitiation of therapy at a lower dose level of sZ

the compound of formula I. No dose-limiting toxicities related to the compound of formula 1 were observed within the tested patient cohorts.

[0434] As shown in Figure 44, almost ail patients with iNHL or CLL who received the combination therapy of bendamustine and Compound I, or rituximab and Compound I experienced réductions in nodal size.

[0435] Furthermore, high levels of antitumor activity were observed in patients with iNHL and in patients with CLL, whether receiving bendamustine and Compound I, or rituximab and Compound I. Summarized in the table of Figure 45, one patient with iNHL receiving bendamustine and Compound I had a complété response.

[0436] Thus, administration of Compound I in combination with rituximab or bendamustine shows acceptable safety and promising clinical activity in patients with relapsed or refractory indolent B-cell NHL and CLL.

[0437] In a previous study, a single-agent therapy that involved administering the compound of formula I caused a lymphocyte redistribution that resulted in lymphocytosis in ~2/3 of patients with CLL, as seen in Figure 46. In contrast, as shown in Figure 47, the combination therapy with bendamustine and Compound I, or rituximab and Compound I resulted in a decrease in malignant lymphocyte counts in ail patients with CLL.

[0438] Thus, the combination therapy dîscussed above provides surprising effects and superior results with little side effects in the réduction of nodal size and anti-tumor activity in patients having iNHL and CLL, as well as a decrease in malignant lymphocyte counts in patients having CLL.

Example 40

Effect of Compound in combination with Bendamustine on B-cell receptor (BCR) signaling and the prosurvival actions of nurse-like cells (NLC), in chronic lymphocytic leukemia [0439] This example demonstrates the effects of the compound of formula I in combination with bendamustine on BCR-derived CLL cell activation. BCR cross-linking with anti-IgM was found to hâve significantly increased CLL cell viability to 121 ± 5 % of controls (mean ± SEM, n=15, *P< 0.05). Thîs pro-survival effect was abrogated by the compound of formula I, which reduced CLL cell viability to 85 ± 3 % of controls at 48 hours (mean ± SEM, n=15, *P< 0.05).

CLL cell viability in co-culture with NLC was also significantly reduced by the compound of

formula I to 64 ± 6% of untreated controls at 48 hours (mean ± SEM, n=10, *P< 0.05). BCR cross-linking induces sécrétion of the chemokines CCL3 and CCL4 by CLL cells, which was quantifîed in CLL supematants by ELISA.

[0440] The compound of formula I significantly reduced supernatant CCL3 concentrations from 4060 ± 1392 pg/mL to 2901 ± 1220 pg/mL, and CCL4 levels from 5721 ± 1789 pg/mL to 3223 ± 1311 pg/mL (mean ± SEM, n=6, *P<0.05). In CLL-NLC co-cultures, the compound of formula I also inhibited CCL3/4 sécrétion by CLL cells. The CCL3 concentrations were reduced by the compound of formula I from 943 ± 535 pg/mL to 156 ± 8 pg/mL, and the CCL4 concentrations from 7433 ± 4463 pg/mL to 316 ± 53 pg/mL (mean ± SEM, n=5, *P<0.05).

[0441] Surprisingly, the compound of formula I also decreased CXCL13 levels in CLL-NLC co-cultures from 151 ± 35 to 70 ± 27 pg/mL (mean ± SEM, n=4, *P=0.05), indicating that the compound of formula I has pharmacological actions on both, CLL cells and the CLL *

microenvironment as represented b y the N LC.

[0442] This example also demonstrates that a combination of the compound of formula I with 15 bendamustine can overcome stroma-mediated drug résistance in CLL cells co-cultures with marrow stromal cells (MSC). As shown in Figures 48a and 48b, the combination of both drugs indicates an increased effect on CLL cell death.

[0443] Furthermore, phospho-flow was used to demonstrate that the compound of formula I inhibits constitutive and BCR-induced PI3K pathway activation in samples obtained from CLL 20 patients undergoing treatment with the compound of formula I. The compound of formula I treatment down-regulated pAkt(T308) in peripheral CLL cells by >90% (n=12). Plasma samples from 14 CLL patients obtained before and after 28 days of daily treatment with the compound of formula I were analyzed for concentrations of various cytokines. Interestingly, these analyses revealed substantial decreases from baseline to Day28+ of the compound of formula 1 treatment 25 in mean plasma levels of CCL3 (from 186 pg/mL to 29 pg/mL), CCL4 (from 303 to 70 pg/mL),

CCL22 (1067 to 533 pg/mL), CXCL13 (316 pg/mL to 40 pg/mL), and TNFa (104 to 29 pg/mL), confirming our in vitro data related to CCL3/4 and CXCL13.

[0444] Collectively, the results from this example show that the compound of formula I effectively inhibits BCR- and NLC-mediated CLL cell survival and activation in vitro. Also, the compound of formula I enhances the activity of cytotoxic agents such as bendamustine against

CLL cells. In vivo data indicate that the compound of formula I decreases elevated pre-treatment v/·

CCL3 and CCL4 levels. While not being bound by theory these observations are suggest the concept that inhibition of BCR-derived signais may be a key mechanism of action of the compound of formula I in CLL.

Example 41

Effect of Compound in Combination with Lenalidomide on Activation of the Phosphatidylinositol 3-kinase-<S Pathway in CLL [0445] This example shows the effect of the compound of formula I in combination with lenalidomide on activation of the PI3k-delta pathway in patients with CLL.

[0446] Sample collection and culture conditions: Isolated mononuclear cells were negatively B-cell selected and placed in culture. The compound of formula I was supplied by Calistoga Pharmaceuticals (Seattle, WA). Lenalidomide (Revlimid; Celgene) was obtained and extracted. [0447] Flow cytometry assays: Antibodies to CD20, CD40, CD80, CD86 or IgGl (BD Biosciences, San José CA) were surface stained.

[0448] Immunoblot analysis: Immunoblots were performed. Antibodies included, Anti-AKT, anti-phospho-AKT (Ser473), anti-GSK3p □ anti-phospho-GSK3p (Ser9) (Cell Sîgnaling, Danvers, MA), anti-ρΐΐθδ (Santa Cruz Biotechnology, Santa Cruz, CA), and anti-GapdH (Millipore, Billerica, MA).

[0449] Quantitative RT-PCR: RNA was extracted using TRIzol reagent (Invitrogen) and cDNA was prepared using a SuperScript First-Strand Synthesis System (Invitrogen). Real-Time PCR was performed using pre-designed TaqMan® Gene Expression Assays and ABI Prism 7700 sequence détection systern (Applied Biosystems, Foster City, CA).

[0450] PI3K Assay: PI3K assay was preformed on whole cell lysâtes from CLL cells. The ELISA assay was performed according to the manufacture’s instructions (Echelon Biosciences, Sait Lake City, UT).

[0451] Transfection: CLL cells were transfected. pllOÔ siRNA (Ambion, Austin, Tx) was used at a final concentration of 50nM.

[0452] Immunoglobulin détection: Quantization of IgM was determined. Briefly, lenalidomide-treated or vehicle control-treated CLL cells, were irradiated and placed in culture with target, purified B-cells, in the absence or presence of PWM (5 ug/mL).

[0453] Statistical analysis: Ail reported statistical évaluations were performed by the Center for Biostatistics at OSU with methods previously described 7. P-values at a=0.05 for single comparisons or after adjustment for multiple comparisons were considered significant.

[0454] The results of this example shows that lenalidomide up-regulates CD154 on CLL cells 5 by activation of AKT, IKK and NF-κΒ nuclear translocation with increased mRNA transcription and stabilization, Treatment of CLL cells with the pan-inhibitor LY294002 antagonized this AKT activation.

[0455] To first confirm the specificity of PI3-kinase activation, the direct increase in the enzymatic activity of PI3K following treatment with lenalidomide was determined. CD19+ cells 10 from CLL patients (N=9) treated with or without 0.5 μΜ lenalidomide were examined for PBkinase activity with and without the addition of 1 or 10 μΜ the compound of formula I to the lysate. Results were calculated relative to pg of protein. Signaling with lenalidomide through the PI3K pathway can occur through four catalytic isoforms: ρΙΙΟα, ρΙΙΟβ, ρΙΙΟγ, and pllOÔ. Furthermore, the inhibition of PI3K-Ô via a small molécule inhibitor, the compound of formula

I, was found to prevent the increase in PI3K enzymatic activity (Figure 49A).

[0456] Next, in determining whether the inhibition of PI3K-Ô with the compound of formula I could prevent the increase in downstream phosphorylation of AKT induced by lenalidomide, inhibition of PI3K-Ô by the compound of formula I resulted in prévention of PI3K activity. CD19+ cells from CLL patients (N=6) were incubated with or without 0.5 μΜ lenalidomide and/or 1 or 10 μΜ the compound of formula I for 48 hours. AKT phosphorylation at ser473 was assessed by immunoblot. See Figure 49B.

[0457] To confirm these results, the phosphorylation of GSK3p was evaluated. CD19+ cells from CLL patients (N=4) were incubated with or without 0.5 μΜ lenalidomide and/or 1 or 10 μΜ the compound of formula I for 48 hours. GSK3p phosphorylation at ser9 was assessed by immunoblot. Results are shown from one of four experiments. The lenalidomide treatment was found to lead to an increase in phosphorylation of GSK3p preventable by co-treatment with the compound of formula I, again suggesting a link between PI3K-Ô and lenalidomide dépendent

PI3K activity. See Figure 49C.

[0458] To confirm that these results were in fact due to PI3K-Ô inhibition, PI3K-Ô in CLL cells were knocked down. CD19+ cells from CLL patients (N=3) were transfected with siRNA

V

targeted to PI3K-Ô, ΡΙ3Κ-γ or a nonsense target. pllOô protein expression was assessed by immunoblot. Results are shown from one of three experiments. See Figure 49D, [0459] Lenalidomide was shown to be unable to induce phosphorylation of AKT when PI3K-S was knocked down. CD19+ cells from CLL patients (N=3) were transfected with siRNA targeted to PI3K-Ô, ΡΙ3Κ-γ or a nonsense and then incubated with or without 0.5 μΜ lenalidomide for 48 hours. AKT phosphorylation at ser473 was assessed by immunoblot. Results are shown from one of three experiments. See Figure 49E.

[0460] These findings support that PI3K activation in CLL and the subséquent effects on NFkB and CD154 on CLL cells by lenalidomide utilizes a PI3K-ÔD dépendent pathway.

[0461] Lenalidomide treatment has previously been shown to resuit in activation of CLL cells. To extend these findings, inhibition of ΡΙ3Κ-δ prevented lenalidomide was învestigated to see whether it induced up-regulation of other co-stimulatory molécules important to B-cell antigen présentation. CD19+ cells from CLL patients (N=25) were treated with or without 0.5 μΜ lenalidomide and/or 10 μΜ the compound of formula I for 48 hours. Surface expression of CD40 or CD86 was evaluated by flow cytometry using a CD40- or CD86-PE antibodies and IgGl-PE isotype control. Treatment with the compound of formula I was able to prevent the upregulation of CD40 and CD86 induced by lenalidomide (p-value=0.0102 and <0.0001, respectively) (Figure 50A).

[0462] Similarly, inhibition of PI3K-Ô was also found to prevent the increase in mRNA of CD40, CD86, CD154 and CD80 promoted by lenalidomide (p-value<0.009 for ail genes) (Figure 50B). CD19+ cells from CLL patients (N=15) were treated with or without 0.5 μΜ lenalidomide and/or 10 μΜ the compound of formula I for 48 hours. RNA was extracted and converted to cDNA and RT-PCR analysis was done to détermine quantities of CD40, CD86 and CD154 mRNA.

[0463] Lenalidomide has also been shown to induce internalization of CD20, concurrent treatment with the compound of formula I prevented this effect (p-value=0.0057) (Figure 50C).CD19+ cells from CLL patients (N=5) were treated with or without 0.5 μΜ lenalidomide and/or 10 μΜ the compound of formula I for 48 hours. Internalization of CD20 was evaluated by quantifying surface expression of CD20 by flow cytometry using a CD20-PE antibodies and IgG 1-PE isotype control.

[0464] Given the importance of the CD40-CD154 axis for lenalîdomide-treated CLL cells to promote normal B-cells to produce immunoglobulin, the compound of formula I was assessed to see whether it could prevent this occurrence. CD19+ cells from CLL patients (N=6) were treated with or without 0.5 μΜ lenalidomide and/or 10 μΜ the compound of formula I for 48 hours. CLL cells were irradiated (20 Gy) and placed in culture with purified B-cells, in the absence or presence of 5 μg/mL PWM. Quantification of IgM was determined by ELISA analysis. Whereas lenalidomide treated CLL cells previously 5 and hereinhave been shown to have increased IgM levels, pre-treatment of CLL cells with the compound of formula I was found to completely prevent production of IgM (p-value<0.0001) (Figure 50D).

[0465] Lastly, lenalidomide treatment of CLL cells in culture was tested to détermine whether it could promote tumor cell production of vascular endothélial growth factor (VEGF) and basic fibroblast growth factor (b-FGF). CD19+ cells from CLL patients (N=15) were treated with or without 0.5 μΜ lenalidomide and/or 10 μΜ the compound of formula I for 48 hours. RNA was extracted and converted to cDNA and RT-PCR analysis was done to détermine quantities of bFGF. Lenalidomide treatment increased transcriptional up-regulation of b-FGF in ail patients (p-value=0.0004) and that co-incubation of the compound of formula I prevented this upregulation (p-value<0.001) (Figure 50E).

[0466] Similarly, lenalidomide treatment increased transcriptional levels of VEGF in a subset (8 of 13) of patients, which was again réversible by treatment with the compound of formula I (p-value=0.002) (Figure 50F). (F) CD19+ cells from CLL patients (N=13) were treated with or without 0.5 μΜ lenalidomide and/or 10 μΜ the compound of formula I for 48 hours. RNA was extracted and converted to cDNA and RT-PCR analysis was done to détermine quantities of VEGF [0467] The data collectively provided support for the PI3K-6 pathway in lenalidomide25 mediated activation of CLL cells. The potential relevance of these findings are several as related to the use of lenalidomide in CLL. Combination of the compound of formula I, pan-PI3-kinase inhibitors and potentially other agents that antagonize signaling downstream of PI3-kinase with lenalidomide are likely to be antagoniste to the immune modulating properties of this agent. Application of this combination therapy if immune modulation is desired should therefore be approached with caution. In contrast, if non-immune modulatory actions of lenalidomide are desired, application of agents such as the compound of formula I or alternative PI3-kinase inhibitors, in view of their surprising effects, might be attractive for preventing immune

activation, tumor flare, and cytokine release syndrome associated with this treatment. This may be particularly true with cytokines such as VEGF 16-19 and b-FGF 16,20,21 which hâve a protective effect against CLL cells. Indeed, one study of lenalidomîde in relapsed CLL that examined serial cytokine levels during treatment noted that non-responding as compared to responding patients had persistently elevated cytokine levels, including b-FGF 2.

Example 42

Effect of Compound in Combination with Rituximab and/or Bendamustine in Patients with Previously Treated B-cell Malignancies [0468] This example demonstrates the safety and activity of the compound of formula I in combination with rituximab and/or bendamustine in patients with previously treated B-cell malignancies.

[0469] 49 patients were enrolled in the study, including 27 with iNHL and 22 with CLL.

Patient characteristics are summarized in Figure 51. At the baseline, patients had adverse prognostic characteristics, including older age, bulky adenopathy, and refractory disease. Ail patients had received >1 prior treatment regïmen and some had received as many 9 prior regimens. Prior treatment included rituximab, alkylating agents and/or fludarabine; the majority of patients with iNHL had been treated with prior anthracycline-containing therapy. Follow-up was early in many patients, with durations of therapy ranging from to 12 cycles.

[0470] Ail patients received the compound of formula I, either 100 mg orally twice per day (BID) or 150 mg orally twice per day (BID) for as long as the patient was benefitting. Patients also received either rituximab 375 mg/m administered weekly for 8 weeks, starting on Day 1 of Cycle 1, or bendamustine 90 mg/m administered on Days 1 and 2 of each cycle for 6 cycles. Tumor response was evaluated according to standard criteria.

[0471] As shown in Figure 52, Grade >3 adverse events largely comprised background events resulting from pre-existing disease, toxicity from prior therapy, or intercurrent illness. For patients receiving bendamustine and the compound of formula I, Grade >3 adverse events included B-induced myelosuppression. For patients receiving rituximab and the compound of formula I, Grade >3 adverse events were infrequent and not clearly related to the compound of formula I. iNHL-specific transient ALT/AST élévations were observed; these events resolved

upon drug interruption and hâve been successfully managed with reinitiation of therapy at a lower dose level of the compound of formula I. No dose-limiting toxicities related to the compound of formula I were observed within the tested patient cohorts.

[0472] As shown in Figures 53A and 53B, almost ail patients with iNHL or CLL who received the combination therapy of bendamustine and the compound of formula I, or rituximab and the compound of formula I experienced réductions in nodal size.

[0473] Furthermore, high levels of antitumor activity were observed în patients with iNHL and in patients with CLL, whether receiving bendamustine and the compound of formula I, or rituximab and the compound of formula I. Summarîzed in Figure 54, two patients with iNHL receiving bendamustine and the compound of formula I had a complété response.

[0474] In a previous study, a single-agent therapy that involved administering the compound of formula I caused a lymphocyte redistribution that resulted in transient lymphocytosis in patients with CLL, as seen in Figure 55. In contrast, as shown in Figure 56, the combination therapy with bendamustine and the compound of formula I, or rituximab and the compound of formula I resulted în a more rapid decrease in malignant lymphocyte counts in patients with CLL.

[0475] Thus, the combination therapy discussed above provides surprising effects and superior results with little side effects in the réduction of nodal size and anti-tumor activity in patients having iNHL and CLL, as well as a decrease in malignant lymphocyte counts in patients having CLL.

Example 43

Effect of Compound in Combination with Dexamethasone. Bendamustine. and/or Fludarabine in CLL cells Co-Cultured with Marrow Stromal Cells (MSCs) [0476] This example demonstrates the effect of compound of formula I on CLL cells when these cells were co-cultured with MSC in the presence of drugs cytotoxic to CLL cells (e.g., dexamethasone, bendamustine, and fludarabine).

[0477] CLL cells were co-cultured with MSCs in medium alone (control) or in medium containing the indicated concentrations of a compound of formula I, dexamethasone, bendamustine, or fludarabine, or the drugs combined.

[0478] The viable cell population was characterized by bright DiOCô staining and PI exclusion, and was gated in the lower right corner of each contour plot. The percentage of viable cells is displayed above each of these gates. As shown in Figure 57a, the combinations of a compound of Formula I with dexamethasone, bendamustine, or fludarabine indicate an increased 5 effect on CLL cell death.

[0479] Viabilities of drug-treated samples were normalized to the viabilities of control samples at the respective timepoints (100%). Figure 57b displays the means (±SEM) from 9 different patient samples, assessed after 24,48 and 72 hours. CLL cell survival in the presence of MSCs was significantly reduced by combination therapy, with P<.05, as indicated by the asterisks describing the comparison of results from each drug-treated culture to the results from the control culture. For example, after 72 hours, CLL cell viability relative to untreated controls was 93% (±0.6%) with a compound of formula I and 60% (±8.7%) with bendamustine, but was reduced to 42.7% (±11.4%) for the combination of the 2 drugs. Comparable results were seen for the combinations of a compound of formula I and fludarabine, or a compound of formula I 15 and dexamethasone.

[0480] Therefore, these results indicate that a compound of formula I sensitized CLL cells to the cytotoxic agents, presumably by disrupting MSC-derived drug résistance signais.

Example 44

Effect of Compound in Combination with Ofatumumab in CLL [0481] This example summarizes the Phase 1-2 study of repeated cycles (28 days/cycle) of compound I in combination with ofatumumab for the treatment of patients who had previously been treated for CLL.

[0482] Compound I (150 mg 2 times per day [BID]) was co-administered continuously with infusions of ofatumumab given over 24 weeks. Ofatumumab was administered with an initial dose of 300 mg on either Day 1 or Day 2 (relative to the first dose of compound I). One week later, ofatumumab was administered at 1,000 mg every week for 7 doses, then at 1,000 mg every 4 weeks for 4 doses. After completion of the ofatumumab treatment, each subject continued to receive compound I as a single agent at a dose of 150 mg BID as long as the subject was benefitting.

J

[0483] From the entire cohort of 21 patients, démographie and preliminary efficacy data from 11 patients were available. The médian [range] âge was 63 [54-76] years. The majority (9/11; 82%) of patients had bulky adenopathy (ül lymph node measuring a5 cm in longest dimension). The médian [range] number of prior thérapies was 3 [1-6], including prior exposure to alkylating agents (10/11; 90%), rituximab (9/11; 82%), purine analogs (8/11; 72%), alemtuzumab (3/11; 28%) and/or ofatumumab (2/11; 18%). At the data cutoff, the médian [range] treatment duration was 5 [0-7] cycles. Almost ail subjects (9/11; 82%) experienced marked and rapid réductions in lymphadenopathy within the first 2 cycles. Among the 11 patients, 10 were évaluable for response assessment at the end of Cycle 2 or later. Eight patients (80%) met criteria for a response as judged by the investigator based on the criteria published in Hallek M, et al. (Guidelines for the diagnosis and treatment of chronic lymphocytic leukemia: a report from the International Workshop on Chronic Lymphocytic Leukemia updating the National Cancer Institute-Working Group 1996 guidelines. Blood. 2008 Jun 15;111(12):5446-56). One patient had reduced lymphadenopathy meeting criteria for stable disease, and one patient had disease progression. The transient increase in the peripheral lymphocyte counts that was expected with single-agent PI3KÔ inhibition was reduced in magnitude and duration. The réduction of the transient lymphocytosis was induced by the combination of compound I and oftatumumab .

[0484] Preliminary safety data show that the combination treatment had a favorable safety profile and lacked myelosuppression. In addition, pharmacodynamie data revealed that elevated baseline levels of CLL-associated chemokines and cytokines (CCL3, CCL4, CXCL13, and TNFa) were reduced after 28 days of treatment. The results suggest that the combination of compound I with ofatumumab provides a well-tolerated, non-cytotoxic treatment regimen in patients with previously treated CLL.

Example 45

Effect of Compound in Combination with BCL-2 antagonists in CLL [0485] This example shows the effect of compound I in combination with BCL-2 antagonists

ABT-737 and ABT-263 on the stroma-exposed CLL cells.

[0486] CLL cellpurification: Peripheral blood, bone marrow, and lymph node were obtained from consent patients fulfdiing diagnostic and immunophenotypic criteria for CLL. Peripheral blood mononuclear cells (PBMCs) were isolated from blood and tissue samples using the Ficoll-

Paque (GE Healthcare, Waukesha, WI) density gradient centrifugation. Samples were either analyzed fresh or viably frozen in 10% dimethyl sulfoxide (DMSO; Sigma-Aldrich, St. Louis, MO) in fêtai bovine sérum (BD Biosciences, San Diego, CA) and stored in liquid nitrogen and later thawed for analysis. Single cell suspensions were prepared for analysis on a fluorescence activated cell sorting (FACS) machine, and CD19+ CLL cells generally accounted for >85% of analyzed cells.

[0487] Cell Unes: Murine CD154+ L cell line was maintained in RPMI1640 medium supplemented with 10% FBS, 2.05mM L-glutamine (HyClone, Logan, UT), and penicillinstreptomycin (Cellgro, Manassas, VA). The human stromal cell line StromaNKTert was purchased from the Riken cell bank (Tsukuba, Japan) and maintained in alpha-MEM supplemented with 1 μg/mL hydrocortisone, 10% FBS, 10% human sérum (Invitrogen, Grand Island, NY), 2.05-mM L-glutamine, and penicillin-streptomycin, Nurse-like cells (NLCs) were established by suspending PBMC from patients with CLL in complété RPMI 1640 medium with 10% FBS and penicillin-streptomycin-glutamine to a concentration of 10⁷ cells/mL (2 mL total). Cells were grown for 14 days in 24-well plates (Corning Life Sciences).

[0488] CLL cell and stromal cell co-cultures: CLL cells were cultured under standardized conditions on stromal cell lines or primary NLC. Briefly, stromal cells were seeded one day prior to each experiment onto 24-well plates (Corning Life Sciences) at a concentration of 3 x 10⁵ cells/mL/well and incubated at 37°C in 5% CO2. Stromal cell confluence was confîrmed by phase contrast microscopy, and CLL cells were then added onto the stromal cell layer at a concentration of 3 x 10⁶ cells/mL. Cultures were then treated with compounds for the specified time periods. CLL cells were removed for analysis by gentle pipetting with media, and were then washed in PBS prior to analysis. A 24-hour co-culture time point was used unless otherwise indicated.

[0489] Cell viability testing and reagents: CLL cell vîability was determined by analysis of Annexin V-FITC (BD Biosciences, ^San Diego, CA) and Propîdium Iodine (Pl) (Sigma) by FACS. ABT-737, ABT-263, and compound I were stored in DMSO at -20°C until use.

[0490] BH3 profiling: CLL patient peripheral blood, bone marrow, and lymph node samples were analyzed by either the plate-^bosed fluorimetry or FACS method. Briefly, PBMCs from CLL patients were made into single cell suspensions and gently permeabilized using digitonin (0.002%). For the fluorimtery-based method, 100 μΜ JC-1 (Invitrogen) was added at this time and cells were then loaded onto a 384-well plate, with individual wells containing individual

BH3-only peptides. The JC1-BH3 assays were then conducted in triplicate on a Tecan Safire 2 with Ex 545 +/- 20 nM and Em 590 +/- 20 nm with a three-hour time course. For the FACSbased method, single cell suspensions from CLL patient PBMCs were stained using human Fc Block (BD Pharmingen) followed by anti-CD19-V450 (BD Pharmingen) and anti-CXCR4-APC 5 (BD Pharmingen). Cells were washed in PBS and then added into individual FACS tubes, each of which contained an individual BH3-only peptide. Samples were incubated at room température for 30 minutes, 100 μΜ JC-1 was added to each tube, and the samples were incubated for an additional 30 minutes. FACS measurements were conducted on a BD FACS Canto II with lasers at 407, 488, and 633 nm. JC-1 was measured from the 488-nm laser using a

530/30-nm filter (FITC) and a 585/42-nm filter (PE), and the degree of mitochondrial depolarization was calculated using the surrogate of the change in the médian of PE signal. The mitochondrial depolarization reported in response to each BH3 peptide is normalized relative to the médian percentage change in PE fluorescence of the JC-1 dye with a négative control, dimethyl sulfoxide (DMSO) (0%) and a positive control, the mitochondrial uncoupling agent carbonyl cyanide 4-(trifluoromethoxy)- phenylhydrazone (FCCP) (100%).

[0491] Calcein-based adhesion assay; a confluent monolayer of CD154+ L or StromaNKTert cells was generated by plating lxlO⁴ cells/well in 96-well plates for 24 hours. Single cell suspensions of CLL cells at 0.5xl0⁶ cells/ml were then labeled with 1 pg/mL Calceîn-AM (Invitrogen) for 1 hour. Cells ^wcre then spun down and treated with compound or vehicle for 1 to

24 hours. Non-adherent cells were washed off by aspiration. CLL cell adhesion was quantified by fluorimetry (Ex/Em = 485/520 nm), and visualized directly using the Nikon TE2000 inverted live-cell imaging system.

[0492] Data analysis and statistics; Results are shown with standard error of mean and number of replicates as described in each figure. Student’s paired or unpaired t-tests, Mann25 Whitney U test, or linear régression analyses were used for statistical comparisons. Analyses were performed with GraphPad Prism 5 software for PC (GraphPad Software, San Diego, CA).

Flow cytometry data were analyzed using FACS Diva version 6.1.1 (BD Pharmingen). Clinical response was assessed using 2008 IW-CLL criteria with responders defined as patients achieving a complété or partial response as best response, and non-responders as patients with stable disease, refractory disease, or progressive disease within 6 months of finishing first therapy. A two-tailed p-value < 0.05 was considered statistically significant unless otherwise indicated.

[0493] Currently, many patients receiving the first-line traditional CLL therapy often relapse and develop résistant to their treatment. Because CLL cells exposed to various stroma are résistant to treatment with both cytotoxic chemotherapy (Kurtova AV et al. Diverse marrow stromal cells protect CLL cells from spontaneous and drug-induced apoptosis: development of a 5 reliable and reproducible system to assess stromal cell adhesion-mediated drug résistance.

Blood. 2009; 114(20):4441-4450) and BH3-mimetics such as ABT-737 or ABT-263 (Vogler M et al. Concurrent up-regulation of BCL-XL and BCL2A1 induces approximately 1000-fold résistance to ABT-737 in chronic lymphocytic leukemia. Blood. 2009; 113(18):4403-4413), CLL cells in the stromal microenvironment may receive proliférative or anti-apoptoic signais from stroma and become protected from cell apoptosis. Thus, agents that antagonize the interactions may reduce the stroma-mediated résistance in CLL.

[0494] The compound of formula I may modulate the stromal microenvironment. In clinical studies, most patients treated with compound I and other agents targeting the BCR pathway exhibited a rapid and transient lymphocytosis. Without being bound to any theory, compound I 15 may modulate the stromal microenvironment by inhibiting CLL cell chemotaxis towards

CXCL12/13, reducing CLL cell migration beneath stromal cells, down-regulating chemokine sécrétion, or inhibiting phosphorylation of other downstream targets such as AKT and ERK. To characterize whether compound I modulate the CLL-stroma interaction by increasing mitochondrial apoptosis or priming, the BH3 profiling was used to measure the permeabilization 20 of mitochondria induced by peptides derived from the pro-death BH3 domains of pro-death

BCL-2 family proteins.

[0495] First, CLL cells from the peripheral blood of 30 patients were examined. Most patients had not been treated previously, and none had recently received therapy. At a final concentration of 0.03 μΜ in the BH3 profiling, BIM BH3 peptide induced a signifîcant amount of depolarization in most patient samples, with 22/30 (73.3%) of samples having >50% depolarization by 1 hour. As shown in Figure 58A, CLL cells were highly primed for apoptosis. Also, the results of BH3 profiling showed that most CLL patient samples showed relatively increased depolarization from BAD BH3 peptide, suggesting primary dependence on BCL-2 (n=23). As shown in Figure 58B, some samples were observed to be more dépendent on MCL-1 (n=5), or BCL-XL (n=2). As shown in Figure 58C, pre-treatment samples from treatment-naïve patients achieving a partial response (PR) or complété response (CR) by 2008 IW-CLL criteria were observed to be more primed than samples from patients with progressive disease (PD)

100 during or within 6 months of completing frontline CLL therapy (p=0.024). As shown in Figure 58D, BH3 profiling shows that patients with unmutated IGHV status (n=7) were significantly more primed than patients with mutated IGHV status (n=18) (p=0.0026). As shown in Figure 58E, percentage of VH homology to germline was observed to be positively correlated with level of priming (p=0.0043). Thus, it was observed that CLL cells were highly primed for apoptosis, that CLL cells were usually BCL-2 dépendent, and that increased priming was associated with improved clinical response and unmutated IGHV.

[0496] Next, the effects of compound I on the adhesion, viability, and priming of the stromaexposed CLL cells in vitro were evaluated. Figures 59A-E generally show that compound I was observed to release CLL cells sequestered in stroma to overcome stroma-mediated résistance. Peripheral blood-derived CLL cells were labeled with calcein-AM and co-cultured on stromaNKTert for 24 hours with or without compound I (10 μΜ), rinsed by gentle pipetting, and visualized by wide-field microscopy. As shown in Figure 59A, CLL cells co-cultured with stromaNKTert and treated with compound I exhibited less adhèrent at 24 hours. Also, Figure 59B showed that the reduced adhérence of CLL cells was détectable even after only one hour treatment of compound I, which was before CLL cell death would occur. Moreover, Figure 59C showed that the de-adherence of CLL cells from stroma in response to compound I resulted in enhanced killing of CLL cells. In particular, mean percent viability for two patients was depicted along with SEM in Figure 59C, and both of these patients demonstrated profound stroma-mediated résistance to either ABT-737 at 100 nM or compound I at 10 μΜ alone. This résistance was observed to be overcome by the combination of the two compounds.

[0497] To avoid examine the direct killing of CLL cells by compound I, two CLL patient samples that were résistant to both ABT-737 and compound I were treated in the presence of stroma. In both samples, compound I restored sensitivity of CLL cells to ABT-737 in the presence of stroma. In addition, stroma-exposed CLL cells treated with compound I (10 μΜ) in combination of various doses of ABT-737 and its oral analogue ABT-263 showed that stromaexposed CLL cells exhibited a dose-dependent increase in killing with either BH3 mimetic. In particular, with reference to Figure 59D, résistance to ABT-737 was observed in the presence of StromaNKTert, but may be overcome with concentrations of ABT-737 as low as 10 nM. With reference to Figure 59E, ABT-263 had a similar dose-response curve.

[0498] To détermine whether PI3K inhibition increased the sensitivity of stroma-exposed CLL cells by increasing priming, PB-derived CLL cells cultured with or without StromaNKTert cells

101 for 24 hours and were examined using Annexin-PI and BH3 profiling. Untreated CLL cells generally exhibited apoptosis in ex vivo culture over 24 hours (Collins RJ et al. Spontaneous programmed death (apoptosis) of B-chronic lymphocytic leukaemia cells following their culture in vitro. British journal ofhaematology. 1989; 71(3):343-350). Stromal co-culture of four CLL patient samples Ied to protection from apoptosis in untreated cells. In particular, with reference to Figure 60A, a two-way ANOVA analysis showed that stroma provided protection from apoptosis in the absence of compound I. In the absence of stroma, compound I was observed to induce more apoptosis than the control. In the presence of stroma, compound I was observed to induce significantly more apoptosis than the control. Thus, no significant différence was observed between killing by compound I in the presence or absence of stroma.

[0499] However, the résistance or protection from apoptosis was reversed by compound I. More than 40% of apoptosis were detected in stroma-exposed CLL cells treated with compound

I (10 μΜ) compared to less than 10% of apoptosis in untreated stroma-exposed CLL cells. Also, as shown in Figure 60B, the BH3 profiling showed that stroma-exposed CLL cells treated with compound I exhibited an increased mitochondrial priming at 24 hours compared to untreated cells (p=0.075). As shown in Figure 60C, both BAD BH3 peptide and ABT-737 used as a peptide induced significantly more mitochondrial depolarization in CLL cells treated with compound I (p=0.046 and p=0.047, respectively). This suggests that the treatment with compound I results in de-adherence of CLL from stroma, accompanied by increased mitochondrial priming and increased sensitivity to BCL-2 antagonism.

[0500] Overall, this example suggested that PI3K inhibition antagonized the protection of CLL cells by stroma, and that compound I was effective at reversing the effects of stroma on CLL cells: adhesion, decreased mitochondrial priming, and decreased sensitivity to thérapies that inhibit BCL-2. Also, the efficicay of compound I may be associated with lymphocyte redistribution in patients. By releasing CLL cells from stroma, compound I likely allowed CLL cells to emerge from the anti-apoptotic stromal milieu, thereby increasing their mitochondrial priming and being susceptible to apoptosis. This example also suggested the combinations of PI3K inhibition with BCL-2 inhibition increase the responses to BCL-2 inhibition.

Claims (18)

1. Use of at least one of a compound of formula A, wherein R is H, halo, or C1-C6 alkyl; R’ is C1-C6 alkyl, or a pharmaceutically acceptable sait thereof; and one or more therapeutic agents selected from the group consisting of bendamustine, rituximab, lenalidomide and ofatumumab; and optionally a pharmaceutically acceptable excipient,

10 in the manufacture of a pharmaceutical composition for the treatment of cancer in a subject..

or a pharmaceutically acceptable sait thereof.

103

3. The use according to claim 1, wherein the cancer is a hematological malignancy.

4. The use according to claim 3, wherein the hematological malignancy is selected from B-cell malignancy and Ieukemia or lymphoma.

5. The use according to claim 1, wherein the cancer is selected from chronic lymphocytîc Ieukemia (CLL) or non-Hodgkin’s lymphoma (NHL) and indolent nonHodgkin’s lymphoma (iNHL).

6. The use according to claim 1, wherein the compound of formula A, or a pharmaceutically acceptable sait thereof, and the one or more therapeutic agents are each for administration at least once during at least one cycle, and wherein the one or more therapeutic agents are for administration to the subject in the same or different cycle as the administration of the compound.

7. The use according to claim 6, wherein the cycle is 7 to 42 days.

8. The use according to claim 6, wherein the one or more therapeutic agents are for administration to the subject on at least the first and second days of at least one cycle.

9. The use according to claim 6, wherein the one or more therapeutic agents are for administration weekly to the subject during at least one cycle.

10. The use according to any one of claims 1-5, wherein said administration comprises administration of between 50 mg and 200 mg of the compound of formula A or a pharmaceutically acceptable sait thereof to the subject twice per day.

11. The use according to any one of claims 1-5, wherein said administration comprises administration of between 50 mg/m² and 1,500 mg/m² of the one or more therapeutic agents.

12. The use according to any one of claims 1-5, wherein the pharmaceutical composition comprises the compound of formula A, or a pharmaceutically acceptable sait thereof, and at least one pharmaceutically acceptable excipient.

104

13. The use according to any one of claims 1-5, wherein the subject is résistant to standard chemotherapeutic treatments.

14. The use according to any one of claims 1-5, wherein the subject has at least one enlarged lymph node.

15. The use according to any one of claims 1-5, wherein the subject is i) refractory to at least one chemotherapy treatment, or ii) is in relapse after treatment with chemotherapy, or a combination thereof.

16 Use of at least one of a compound of formula A, wherein R is H, halo, or C1-C6 alkyl; R’ is C1-C6 alkyl; or a pharmaceutically acceptable sait thereof; and one or more therapeutic agents selected from the group consisting of bendamustine, rituximab, lenalidomide and ofatumumab; and optionally a pharmaceutically acceptable excipient, in the manufacture of a pharmaceutical composition for the treatment of a subject with a B-cell disorder by administration of said composition to said subject wherein said treatment comprises the prior step of identifying the subject having the Bcell malignancy, wherein the subject is refractory to or is in relapse after at least one or more treatments selected from the group consisting of bortezomib (Velcade®), carfilzomib (PR-171), PR-047, disulfiram, lactacystin, PS-519, eponemycin, epoxomycin, aclacinomycin, CEP-1612, MG-132, CVT-63417, PS-341, vinyl sulfone tripeptide inhibîtors, ritonavir, PI-083, (+/-)-7-methylomuralide, (-)-7-methylomuralide, perifosine, rituximab, sildenafil citrate (Viagra®), CC-5103, thalidomide, epratuzumab (hLL2- antiCD22 humanized antibody), simvastatin, enzastaurin, Campath 1H®, dexamethasone, DT P ACE, oblimersen, antineoplaston A10, antineoplaston AS2 1, alemtuzumab, beta alethine, cyclophosphamide, doxorubicin hydrochloride, PEGylated liposomal

105 doxorubicm hydrochloride, prednisone, prednisolone, cladribine, vincristine sulfate, fludarabine, filgrastim, melphalan, recombinant interferon alfa, carmustine, cisplatin, cyclophosphamide, cytarabine, etoposide, melphalan, dolastatin 10, indium In 111 monoclonal antibody MN-14, yttrium Y 90 humanized epratuzumab, anti-thymocyte globulin, busulfan, cyclosporine, methotrexate, mycophenolate mofetîl, therapeutic allogeneic lymphocytes, Yttrium Y 90 ibritumomab tiuxetan, sirolimus, tacrolimus, carboplatin, thiotepa, paclitaxel, aldesleukin, recombinant interferon alfa, docetaxel, ifosfamide, mesna, recombinant interleukin-12, recombinant interleukin-11, Bcl-2 family protein inhibitor ABT-263, denileukin diftitox, tanespimycin, everolimus, pegfîlgrastim, vorinostat, alvocidib, recombinant flt3 ligand, recombinant human thrombopoietin, lymphokine-activated killer cells, amifostine trihydrate, aminocamptothecin, irinotecan hydrochloride, caspofungin acetate, clofarabine, epoetin alfa, nelarabine, pentostatin, sargramostim, vinorelbine ditartrate, WT-1 analog peptide vaccine, WT1 126-134 peptide vaccine, fenretinide, ixabepilone, oxaliplatin, monoclonal antibody CD19, monoclonal antibody CD20, omega-3 fatty acids, mitoxantrone hydrochloride, octreotide acetate, tositumomab and iodine 1131 tositumomab, motexafin gadolinium, arsenic trioxide, tipifarnib, autologous human tumor-derived HSPPC-96, veltuzumab, bryostatin 1, antiCD20 monoclonal antibodies, chlorambucil, pentostatin, lumiliximab, apolizumab, AntiCD40, ofatumumab, temsirolimus, bendamustine, purine analogs, lenalidomide, an alkylating agent, and anthracycline-containing therapy, or a combination thereof.

17. The method according to claim 16, wherein the subject is refractory or is in relapse after at least one or more treatments selected from the group consisting of rituximab, an alkylating agent, fludarabine, and an anthracycline-containing therapy, and a combination thereof.

18. The use according to claim 16, wherein said administration comprises administration of between 50 mg and 200 mg of the compound of formula (A) or a pharmaceutically acceptable sait thereof to the subject twice per day during at least one or more cycles.

19. The use according to claim 16 or 17, wherein said administration comprises administration of between 50 mg/m² and 1,500 mg/m² of the one or more additional therapeutic agents to the subject at least once during at least one or more cycles, wherein

106 the one or more therapeutic agents are for administration to the subject in the same or different cycle as the administration of the compound of formula A or a pharmaceutically acceptable sait thereof.