NZ791717A

NZ791717A - Use of pridopidine for treating functional decline

Info

Publication number: NZ791717A
Application number: NZ791717A
Authority: NZ
Inventors: Beth Borowsky; Eli Eyal; Igor D Grachev; Michael Hayden; Spyridon Papapetropoulos; Juha Matti Savola
Original assignee: Prilenia Neurotherapeutics Ltd
Priority date: 2016-08-24
Filing date: 2017-08-24
Publication date: 2022-08-26

Abstract

This invention provides use of pridopidine or a pharmaceutically acceptable salt thereof in preparation of a medicament for improving or maintaining motor ability, improving or maintaining mobility or reducing or maintaining involuntary movements in a human subject, wherein the human subject is afflicted with early stage Huntington’s disease. icted with early stage Huntington’s disease.

Description

USE OF PRIDOPIDINE FOR TREATING FUNCTIONAL DECLINE This application is a divisional of New Zealand Patent Application No. 772133, the entire contents of which are hereby incorporated by reference in their entirety.

Throughout this application, various publications are referred to by first author and year of publication. Full citations for these publications are presented in a References section immediately before the claims. Disclosures of the publications cited in the References section are hereby incorporated by reference in their entireties into this ation in order to more fully describe the state of the art as of the date of the ion described herein.

OUND OF ION Huntington’s disease Huntington’s disease (HD) is a fatal neurodegenerative disorder with an autosomal dominant mode of tance. The disease is associated with a triad of motor, behavioral, and cognitive symptoms.

Motor disturbances are the ng e of the disease, with chorea the most evident motor symptom. Although useful for diagnosis, chorea is a poor marker of disease severity. Rather, disability and disease severity best correlate with negative motor features such as impairment in fine motor skills, bradykinesia, and gross motor coordination , including speech difficulties, gait, and postural dysfunction (Mahant 2003). ne is widely regarded as an important neurotransmitter modulating several aspects of brain functions including motor on (Nieoullon 2003). A disrupted dopaminergic signaling has been implicated in a number of neurological and psychiatric conditions, (Zhan 2011, Dunlop 2007) and there is considerable clinical and preclinical evidence suggesting that dopaminergic functions are also compromised in HD (Kung 2007, Huot 2007).

A number of medications are prescribed to ameliorate the motor and emotional problems associated with HD; r, the scientific evidence for the usefulness of various drugs in HD is poor (Mestre 2009, Mestre 2009). Only tetrabenazine and rabenazine, which reduce dopamine availability and transmission, are registered specifically for the treatment of patients with HD for the ment of chorea. No registered drugs are ble for the management of the multifaceted symptoms of HD resulting in inexorable functional capacity decline throughout the course of the disease. As such, there is a significant unmet medical need to develop medications to retard or ameliorate functional deficits in HD.

Pridopidine Pridopidine (methylsulfonyl)phenyl]propyl-piperidine) (formerly known as ACR16) is a drug under development for treatment of Huntington’s e. Pridopidine has been shown to modulate motor activity by either suppressing hyperactivity or enhancing hypoactivity. The neuroprotective properties of idine are suggested to be attributed to its high affinity to the sigma-1 receptor (S1R, binding IC50 ~ 100nM), while the motor activity of pridopidine may be mediated primarily by its low-affinity, antagonistic activity at the dopamine D2 receptor (D2R) (binding IC50 ~ 10μM) (Ponten 2010). idine shows low-affinity binding to onal receptors in the micromolar range.

The S1R is an endoplasmic reticulum (ER) chaperone protein which is implicated in cellular differentiation, lasticity, neuroprotection and ive function in the brain. Recently, transcriptomic analysis of rat striatum showed that pridopidine treatment activates expression of the BDNF, dopamine or 1 (D1R), glucocorticoid receptor (GR), and the serine-threonine kinase protein kinase B (Akt)/phosphoinositide 3-kinase (PI3K) pathways, known to promote neuronal plasticity and survival and to be impaired in HD. Moreover, pridopidine gene expression profile showed a reversed pattern of the HD disease gene expression e in a Q175 knock-in (Q175 KI) HD mouse model (Geva 2016). Pridopidine also enhances secretion of the neuroprotective brainderived neurotrophic factor (BDNF) in a neuroblastoma cell line, in a S1R-dependent manner (Geva 2016).

BRIEF SUMMARY OF THE INVENTION This invention provides use of pharmaceutical ition comprising pridopidine or a pharmaceutically acceptable salt thereof in the preparation of a medicament for reducing or maintaining the concentration of neurofilament light protein in an early-stage Huntington disease (HD1 and HD2, TFC 7-13) patient.

This invention provides a method of maintaining functional capacity, ing functional capacity, or lessening the decline of functional capacity in a human patient comprising periodically orally administering to the patient a pharmaceutical composition comprising pridopidine such that a dose of 90-225 mg of pridopidine is administered to the patient per day, so as to thereby maintain functional capacity, improve functional capacity, or lessen the decline of functional capacity in the human patient. In some embodiments the method includes a dose of 90 mg or 180 mg of pridopidine administered to the patient per day. In some embodiments the method includes a dose of 90 mg of pridopidine administered to the patient per day. In some embodiments the patient is a Huntington’s disease (HD) patient.

This invention provides a method of maintaining functional capacity, improving onal capacity, or reducing the rate of decline of functional capacity in a human patient comprising periodically orally administering to the t a pharmaceutical composition comprising pridopidine such that a dose of 90-225 mg of pridopidine is stered to the patient per day, so as to thereby maintain functional capacity, improve onal capacity, or reduce the rate of decline of functional ty in the human patient. In some embodiments the method includes a dose of 90 mg or 180 mg of pridopidine administered to the patient per day. In some embodiments the method includes a dose of 90 mg of pridopidine administered to the patient per day. In some embodiments the patient is a HD patient.

The invention onally provides a method of slowing the clinical ssion of HD as measured by total onal capacity in a human patient comprising periodically orally administering to the patient afflicted with HD a pharmaceutical ition comprising idine such that a dose of 90- 225 mg of idine is administered to the patient per day, so as to thereby slow the clinical progression of HD in the patient as measured by total functional ty. In some embodiments the method includes a dose of 90 mg or 180 mg of pridopidine stered to the patient per day. In some embodiments the method includes a dose of 90 mg of pridopidine administered to the patient per day. In some embodiments the 90 mg daily dose is administered to the t as 45 mg bid.

Further ed is a method of decreasing functional decline in a human HD patient comprising periodically orally administering to the patient a pharmaceutical composition comprising pridopidine such that a dose of 90-180 mg of pridopidine is administered to the patient per day, so as to thereby decrease the functional decline in the patient. In some embodiments, functional decline from baseline in comparison to placebo (a HD subject not receiving pridopidine) is decreased by at least 10%, by at least 15%, by at least 20%, by at least 25%, by at least 30%, by at least 35% or by at least 40%. In some embodiments the method includes a dose of about 90mg to about 180 mg of pridopidine administered to the patient per day. In some embodiments the method includes a dose of 90 mg of pridopidine administered to the patient per day. In some embodiments of the method, the 90 mg dose is stered to the patient as 45 mg bid. In some embodiments of the method, the idine is administered . In some ments of the method, the administration continues for at least 26 weeks, at least 52 weeks, about 78 weeks or at least 78 weeks. In some embodiments of the method, the HD patient is an adult patient. HD patient is classified as an early stage patient, for example, as a stage 1 or stage 2 HD (HD1 or HD2) patient. In some embodiments, the patient has a baseline TFC score of 7-13 or at least 7, at least 8, at least 9, at least 10, at least 11, at least 12, 13 or 7-10 or 11-13.

In some embodiments, functional capacity of a patient is ed using the Total Functional ty (TFC) scale of the Unified Huntington’s Disease Rating Scale (UHDRS), UHDRS-TFC or TFC. In some embodiments of the method, the patient’s baseline functional capacity and one or more uent functional capacity assessments is performed to determine any change in onal decline.

Further provided is a method of achieving a reduced change from baseline in the UHDRS-TFC score in a human HD patient comprising periodically orally administering to the patient a ceutical composition comprising pridopidine such that a dose of 90-180 mg of pridopidine is stered to the patient per day, so as to thereby effect a change in the UHDRS-TFC score in the patient when compared to a HD subject not receiving pridopidine. In some embodiments the method includes a dose of about 90 mg to about 180 mg of pridopidine administered to the patient per day. In some embodiments the method includes a dose of 90mg of pridopidine administered to the t per day.

In some embodiments of the method, the administration continues for at least 26 weeks, or at least 52 weeks or about 78 weeks or at least 78 weeks. In some embodiments of the method, the HD t is classified as a stage 1 or stage 2 HD patient based on the t’s UHDRS-TFC score. In some embodiments, the patient has a baseline TFC score of 7-13 or at least 7, at least 8, at least 9, at least , at least 11, at least 12, 13 or 7-10 or 11-13. In some embodiments of the method, the difference in change from baseline in the UHDRS-TFC score, when compared to a HD subject not receiving pridopidine is reduced by at least 0.2 points over a period of 26 weeks or by at least 0.3 points over 52 weeks or by 0.5 over 78 weeks. In some embodiments of the method, the difference in change from baseline in the UHDRS-TFC score, when compared to a HD subject not receiving pridopidine, is a decrease in the rate of TFC decline by at least 20%, by at least 30% by at least 40% or by at least 50% at 78 weeks.

In some embodiments of the methods disclosed herein, TFC includes one or more of ining tion, taking care of finances, domestic chores, requiring low level of care and ties of daily living (ADL).

The invention additionally provides a method of achieving a reduced change from baseline in the Timed Up and Go (TUG) test in a human HD t comprising periodically orally administering to the patient a ceutical composition comprising idine such that a dose of 90-180 mg of idine is administered to the patient per day, so as to thereby reduce the change in the TUG test in the patient compared to a HD subject not receiving pridopidine.

The ion additionally provides a method of achieving a reduced change from baseline in the TUG test in a human HD patient comprising periodically orally administering to the patient a pharmaceutical composition comprising pridopidine such that a dose of 90-180 mg of pridopidine is stered to the patient per day, so as to thereby reduce the change in the TUG test in the patient compared to a HD t not receiving pridopidine.

The invention additionally provides a method of achieving a d change from baseline in the Symbol Digit Modalities test (SDMT) test in a human HD patient comprising periodically orally administering to the patient a pharmaceutical composition comprising pridopidine such that a dose of 90-180 mg of pridopidine is administered to the patient per day, so as to thereby reduce the change in the SDMT test in the patient compared to a HD subject not receiving pridopidine.

The invention additionally provides a method of achieving a reduced change from baseline in the Stroop Word test in a human HD patient comprising periodically orally administering to the patient a pharmaceutical composition comprising idine such that a dose of 90-180 mg of pridopidine is administered to the patient per day, so as to thereby reduce the change in the Stroop Word test in the patient compared to a HD subject not receiving pridopidine.

The invention additionally provides a method of achieving a reduced change from baseline in the UHDRS-Independence Scale -IS) in a human HD patient comprising periodically orally administering to the patient a pharmaceutical composition comprising pridopidine such that a dose of 90-180 mg of pridopidine is administered to the patient per day, so as to thereby reduce the change in the UHDRS-IS in the t compared to a HD subject not receiving pridopidine.

The invention additionally provides a method of achieving a reduced change from baseline in the gait and balance score as defined by the sum of the Total Motor Score (UHDRS-TMS) domains gait, tandem walking and retropulsion pull test in a human HD patient comprising periodically orally administering to the patient a pharmaceutical composition comprising pridopidine such that a dose of 90-180 mg of pridopidine is administered to the patient per day, so as to thereby reduce the change in the gait and balance score in the patient compared to a HD subject not receiving pridopidine.

The ion onally provides a method of achieving a reduced change from baseline in the UHDRS-TMS chorea subscore in a human HD patient comprising periodically orally administering to the patient a pharmaceutical composition comprising pridopidine such that a dose of 90-180 mg of pridopidine is administered to the patient per day, so as to thereby reduce the change in the UHDRSTMS chorea subscore in the t compared to a HD subject not receiving pridopidine.

This invention also provides a method of maintaining or improving a human patient’s ability to perform activities of daily living comprising periodically orally administering to the patient a pharmaceutical composition comprising pridopidine such that a dose of 90-225 mg of pridopidine is administered to the patient per day, so as to thereby maintain or improve the human patient’s ability to perform ties of daily .

The invention further provides a method of ng dystonia or ining a level of dystonia in a human patient sing periodically orally administering to the patient a pharmaceutical composition comprising pridopidine such that a dose of 90-225 mg of pridopidine is administered to the patient per day, so as to thereby reduce dystonia or in a level of dystonia in the human patient.

The invention also provides a method of treating limb Dystonia in a human patient comprising periodically orally administering to the patient a pharmaceutical composition comprising idine such that a dose of 90-225 mg of pridopidine is stered to the patient per day, so as to thereby treat the limb dystonia in the human patient.

The invention further provides a method of improving or maintaining, a human patient’s gait and balance comprising periodically orally administering to the patient a pharmaceutical ition comprising idine such that a dose of 90-225 mg of pridopidine is administered to the patient per day, so as to thereby improve or maintain, a human patient’s gait and balance.

Additionally provided is a method of improving, maintaining, or slowing the decline of, a human patient’s gait and balance comprising ically orally administering to the patient a pharmaceutical composition comprising pridopidine such that a dose of 90 mg of pridopidine is administered to the patient per day, so as to thereby improve, maintain, or slow the e of, a human patient’s gait and balance.

The invention also provides a method of improving or maintaining, a human patient’s independence comprising periodically orally administering to the patient a ceutical composition comprising pridopidine such that a dose of 90-225 mg of pridopidine is administered to the patient per day, so as to thereby improve or maintain a human t’s independence.

The invention also provides a method of improving, maintaining, or slowing the decline of, a human patient’s independence sing periodically orally administering to the patient a pharmaceutical composition comprising pridopidine such that a dose of 90 mg of pridopidine is administered to the patient per day, so as to thereby improve, maintain, or slow the decline of, a human patient’s independence.

The invention also provides a method of ing or maintaining a human patient’s cognitive domains comprising periodically orally stering to the patient a pharmaceutical composition sing pridopidine such that a dose of 90-225 mg of pridopidine is administered to the patient per day, so as to thereby improve or maintain the human patient’s ive domains.

Further provided is a method of improving, maintaining, or slowing the decline of, a human patient’s cognitive domains comprising periodically orally administering to the patient a pharmaceutical composition comprising pridopidine such that a dose of 90 mg of idine is administered to the patient per day, so as to y improve, maintain, or slow the decline of, a human patient’s cognitive domains. A patient’s cognitive domains may also be the patient’s cognitive performance across a variety of domains The human patient’s cognitive domains may be measured, for example, by the cognitive assessment battery (CAB) and/or the Hopkins Verbal Learning Test – Revised (HVLT-R). The cognitive domains may also be measured by the trail making test B (TMT-B). The cognitive domains may also be measured by the HD Cognitive Assessment Battery (HD-CAB), which includes 6 tests.

The ion also provides a method of reducing the severity of the ned or intermittent muscle ctions associated with dystonia in a human patient comprising periodically orally administering to the patient a pharmaceutical composition sing idine such that a dose of 90-225 mg of pridopidine is stered to the t per day, so as to thereby reduce the severity of the sustained or intermittent muscle contractions associated with dystonia in the human patient. In one embodiment of this method, the patient is afflicted with HD.

The severity of the sustained or intermittent muscle contractions ated with dystonia in a human patient may be measured by, for example, the UHDRS TMS ia score.

Further provided is a method of improving or maintaining motor ability in a human patient comprising periodically orally administering to the patient a pharmaceutical composition comprising pridopidine such that a dose of 90-225 mg of pridopidine is administered to the patient per day, so as to thereby improve motor ability in the human patient.

The motor ability may be measured, for example, by the UHDRS Total Motor Score (TMS) score, the UHDRS TMS score excluding chorea or UHDRS TMS score excluding dystonia.

The invention also provides a method of ng or maintaining the level of chorea in a human patient comprising periodically orally administering to the patient a pharmaceutical composition comprising pridopidine such that a dose of 90-225 mg of pridopidine is administered to the patient per day, so as to thereby reduce or maintain the level of chorea in a human patient.

The level of chorea may also be slowed. Accordingly, the invention provides a method of reducing, maintaining, or slowing the se of, chorea in a human patient comprising periodically orally administering to the patient a pharmaceutical composition comprising pridopidine such that a dose of 90 mg of pridopidine is administered to the patient per day, so as to thereby reduce, maintain, or slow the increase of, chorea in a human patient.

The human patient’s chorea may be measured by the UHDRS TMS chorea score.

The ion further provides a method of improving or maintaining a human patient’s behavior and/or atric state comprising periodically orally stering to the patient a pharmaceutical ition sing pridopidine such that a dose of 90-225 mg of pridopidine is stered to the patient per day, so as to thereby improve or maintain the human patient’s or and/or psychiatric state.

The human patient’s behavior and/or psychiatric state may be measured, for example, by the Problem Behaviors ment total score. The human patient’s behavior and/or psychiatric state may also be measured by the Problem Behaviors Assessment for depressed mood. The human patient’s behavior and/or atric state may also be measured by the Problem Behaviors Assessment for irritability.

The human patient’s behavior and/or psychiatric state may also be measured by the Problem Behaviors Assessment for lack of initiative or apathy. The human patient’s behavior and/or psychiatric state may be measured, for example, by the Problem Behaviors Assessment for obsessive- compulsiveness. The human t’s or and/or psychiatric state may also be measured by the Problem Behaviors Assessment for disoriented behavior.

Further provided is a method of improving or lessening decline of lack of tive or apathy in a human HD patient comprising periodically orally stering to the patient a pharmaceutical composition comprising pridopidine such that a dose of 90-225 mg of pridopidine is administered to the patient per day, so as to thereby improve or lessen decline of lack of initiative or apathy in the patient.

The invention also provides a method of reducing or maintaining a human patient’s involuntary movements comprising periodically orally administering to the t a pharmaceutical composition comprising pridopidine such that a dose of 90-225 mg of idine is administered to the patient per day, so as to thereby reduce or maintain a human patient’s involuntary movements.

The invention r provides method of improving or maintaining a human t’s ty comprising periodically orally administering to the patient a pharmaceutical composition comprising pridopidine such that a dose of 90-225 mg of pridopidine is administered to the patient per day, so as to thereby e or maintain the human patient’s mobility.

This invention also provides a method of improving or maintaining a human t’s y to perform physical tasks comprising periodically orally administering to the patient a pharmaceutical composition comprising pridopidine such that a dose of 90-225 mg of idine is administered to the t per day, so as to thereby improve or maintain the human patient’s ability to perform physical tasks.

In some embodiments of the methods disclosed above, a dose of 90 mg or 180 mg of pridopidine is administered to the patient per day. In some embodiments of the methods disclosed above, a dose of 90 mg of pridopidine administered to the patient per day. In preferred embodiments of the methods disclosed above, the dose of 90 mg of pridopidine administered to the patient per day is stered to the patient as 45 mg bid.

In some embodiments, the patient is administered 45 mg pridopidine qd for about one to two weeks and 45 mg idine bid thereafter. In some embodiments of the methods disclosed above, the administration continues for at least 12 weeks, at least 26 weeks, more than 26 weeks, at least 52 weeks or at least 78 weeks. In some embodiments of the methods disclosed above, the administration continues for 52 weeks or 78 weeks. In some embodiments of the methods disclosed above, the HD t is an early stage HD patient and has a baseline TFC score of at least 7, at least 8, at least 9, at least 10, at least 11, at least 12, 13, or 7-10 or 11-13. In some embodiments of the methods disclosed above, the HD patient has been diagnosed as having at least 36 CAG repeats in the gtin gene. In some embodiments, the HD patient has been diagnosed as having at least 44 repeats in the huntingtin gene. In some embodiments of the methods disclosed above the HD patient is an adult patient and is at least 18 years old or is at least 21 years old. In some embodiments of the methods sed above, the HD patient is an early stage HD patient. In some embodiments the patient is a stage 1 HD (HD1) patient or stage 2 HD (HD2) patient. In some embodiments, the patient is HD1 patient and is experiencing one or more symptom of HD. In some embodiments, the HD patient is not a pre-manifest HD t.

Provided herein is a pharmaceutical composition comprising pridopidine for use in lessening the e of functional ty in a human patient wherein the pharmaceutical composition is to be ically orally administered to the patient such that a dose of 90-225 mg of pridopidine is to be administered to the patient per day. In some embodiments functional capacity is total functional capacity. In some embodiments the daily dose is 90 mg pridopidine. In some embodiments the daily dose is 45 mg bid. ed herein is a pharmaceutical composition comprising pridopidine for use in maintaining functional capacity in a human patient n the pharmaceutical composition is to be periodically orally administered to the patient such that a dose of 90-225 mg of pridopidine is to be administered to the patient per day. In some embodiments functional capacity includes ties of daily living (ADL).

Provided herein is use of an amount of pridopidine in the manufacture of a medicament maintaining functional capacity in a human patient wherein the medicament is formulated for periodic oral administration to the patient such that a dose of 90-225 mg of pridopidine is to be administered to the patient per day. In some embodiments functional capacity includes ADL.

Provided herein is a pharmaceutical ition comprising pridopidine for use in slowing the clinical progression of HD as measured by total functional capacity in a human patient wherein the ceutical composition is to be periodically orally administered to the t such that a dose of 90-225 mg of pridopidine is to be administered to the patient per day. In many embodiments, (a) the pharmaceutical composition is administered for more than 26 weeks or (b) a titration dose of an amount different from the intended dose is administered for a period of time at the start of the periodic administration, or (c) the human patient is afflicted with early stage Huntington’s disease In some embodiments of the ceutical compositions and uses, TFC includes one or more of maintaining occupation, taking care of finances, domestic chores, requiring low level of care and ties of daily living (ADL).

Provided herein is a use of an amount of pridopidine in the manufacture of a medicament for slowing the clinical progression of HD as ed by total functional capacity in a human patient wherein the medicament is formulated for periodic oral administration to the patient such that a dose of 90-225 mg of pridopidine is to be administered to the patient per day.

Provided herein is a ceutical composition comprising pridopidine for use in maintaining a human patient’s ability to m activities of daily living in a human patient wherein the pharmaceutical composition is to be periodically orally stered to the patient such that a dose of 90-225 mg of pridopidine is to be administered to the patient per day.

Provided herein is use of an amount of pridopidine in the manufacture of a medicament for use in maintaining a human t’s ability to perform activities of daily living in a human patient wherein the medicament is formulated for periodic oral administration to the patient such that a dose of 90-225 mg of pridopidine is to be administered to the t per day.

Provided herein is a pharmaceutical composition comprising pridopidine for use in reducing ia or maintaining a level of dystonia in a human t wherein the pharmaceutical composition is to be periodically orally administered to the patient such that a dose of 90-225 mg of pridopidine is to be administered to the patient per day. In some embodiments dystonia es limb dystonia.

Provided herein is use of an amount of pridopidine in the manufacture of a ment for use in reducing dystonia or maintaining a level of dystonia in a human patient wherein the medicament is ated for periodic oral stration to the patient such that a dose of 90-225 mg of pridopidine is to be administered to the patient per day. In some embodiments dystonia includes limb dystonia.

Provided herein is a pharmaceutical composition comprising pridopidine for use in treating limb dystonia in a human patient wherein the pharmaceutical composition is to be periodically orally administered to the t such that a dose of 90-225 mg of pridopidine is to be stered to the patient per day.

Provided herein is use of an amount of pridopidine in the manufacture of a medicament for use in treating limb dystonia in a human patient wherein the medicament is formulated for periodic oral administration to the patient such that a dose of 90-225 mg of pridopidine is to be administered to the patient per day.

Provided herein is a pharmaceutical ition comprising pridopidine for use in improving or maintaining gait and balance in a human patient wherein the pharmaceutical composition is to be periodically orally stered to the patient such that a dose of 90-225 mg of pridopidine is to be stered to the patient per day. In some embodiments the administration slows the decline of a patients gait and e.

Provided herein is use of an amount of pridopidine in the manufacture of a medicament for use in improving or maintaining, a human patient’s gait and balance in a human patient wherein the medicament is formulated for periodic oral administration to the patient such that a dose of 90-225 mg of pridopidine is to be administered to the patient per day. In some embodiments the administration slows the decline of a patients gait and balance.

Provided herein is a ceutical composition comprising pridopidine for use in improving, maintaining, or slowing the decline of gait and balance in a human patient wherein the pharmaceutical composition is to be ically orally administered to the patient such that a dose of 90mg of pridopidine is to be administered to the patient per day. In some embodiments the administration slows the decline of a patients gait and balance.

Provided herein is use of an amount of pridopidine in the manufacture of a medicament for use in improving, maintaining, or slowing the decline of, a human patient’s gait and balance in a human patient wherein the medicament is formulated for periodic oral administration to the patient such that a dose of 90mg of pridopidine is to be administered to the patient per day. In some embodiments the administration slows the decline of a patients gait and balance.

Provided herein is a pharmaceutical composition comprising idine for use in ing or maintaining independence in a human patient wherein the ceutical composition is to be periodically orally administered to the patient such that a dose of 90-225 mg of pridopidine is to be administered to the patient per day.

Provided herein is use of an amount of pridopidine in the manufacture of a medicament for use in improving or ining, a human patient’s independence wherein the ment is formulated for periodic oral stration to the patient such that a dose of 90-225 mg of idine is to be administered to the patient per day.

Provided herein is a pharmaceutical composition comprising pridopidine for use in ing or maintaining or g the decline of a human patient's independence wherein the pharmaceutical composition is to be periodically orally administered to the patient such that a dose of 90 mg of pridopidine is to be stered to the patient per day.

Provided herein is use of an amount of pridopidine in the manufacture of a medicament for use in improving or maintaining, or slowing the decline of a human patient’s independence wherein the medicament is formulated for ic oral administration to the patient such that a dose of 90 mg of pridopidine is to be administered to the patient per day.

Provided herein is a pharmaceutical composition comprising pridopidine for use in improving or maintaining a human patient’s cognitive domains wherein the pharmaceutical ition is to be periodically orally administered to the patient such that a dose of 90-225 mg of pridopidine is to be administered to the patient per day.

Provided herein is use of an amount of pridopidine in the manufacture of a medicament for use in ing or maintaining a human patient’s cognitive domains wherein the medicament is formulated for periodic oral administration to the patient such that a dose of 90 mg of pridopidine is to be administered to the patient per day.

Provided herein is a pharmaceutical composition comprising pridopidine for use in improving or maintaining or slowing the decline of a human patient’s cognitive domains wherein the pharmaceutical composition is to be periodically orally administered to the t such that a dose of 90 mg of idine is to be administered to the patient per day.

Provided herein is use of an amount of pridopidine in the manufacture of a ment for use in improving or maintaining or slowing the decline of a human patient’s cognitive domains n the medicament is formulated for periodic oral administration to the patient such that a dose of 90-225 mg of pridopidine is to be stered to the patient per day.

Provided herein is a pharmaceutical composition comprising pridopidine for use in reducing the severity of the sustained or intermittent muscle contractions associated with ia in a human patient wherein the pharmaceutical composition is to be periodically orally administered to the patient such that a dose of 90-225 mg of pridopidine is to be stered to the patient per day.

Provided herein is use of an amount of pridopidine in the manufacture of a ment for use in reducing the severity of the sustained or intermittent muscle ctions associated with dystonia in a human patient wherein the medicament is formulated for periodic oral administration to the patient such that a dose of 90-225 mg of idine is to be administered to the patient per day. ed herein is a pharmaceutical composition comprising idine for use in improving or maintaining motor ability in a human patient wherein the pharmaceutical composition is to be ically orally administered to the patient such that a dose of 90-225 mg of pridopidine is to be administered to the patient per day.

Provided herein is use of an amount of pridopidine in the manufacture of a medicament for use in improving or maintaining motor ability in a human patient wherein the medicament is formulated for periodic oral administration to the patient such that a dose of 90-225 mg of pridopidine is to be administered to the patient per day.

Provided herein is a pharmaceutical composition comprising pridopidine for use in reducing or maintaining the level of chorea in a human patient wherein the pharmaceutical composition is to be periodically orally administered to the patient such that a dose of 90-225 mg of idine is to be administered to the patient per day.

Provided herein is use of an amount of pridopidine in the manufacture of a medicament for use in reducing or ining the level of chorea in a human patient wherein the medicament is formulated for periodic oral administration to the patient such that a dose of 90-225 mg of pridopidine is to be administered to the patient per day.

Provided herein is a pharmaceutical composition comprising pridopidine for use in reducing or maintaining or slowing the increase of chorea in a human patient wherein the pharmaceutical composition is to be periodically orally administered to the t such that a dose of 90 mg of pridopidine is to be administered to the patient per day.

Provided herein is use of an amount of pridopidine in the manufacture of a medicament for use in reducing or maintaining or slowing the increase of chorea in a human t wherein the medicament is ated for periodic oral administration to the patient such that a dose of 90 mg of pridopidine is to be administered to the patient per day.

Provided herein is a pharmaceutical composition sing pridopidine for use in improving or maintaining a human patient’s behavior and/or atric state wherein the pharmaceutical composition is to be periodically orally administered to the patient such that a dose of 90-225 mg of pridopidine is to be administered to the patient per day.

Provided herein is use of an amount of pridopidine in the manufacture of a medicament for use in improving or maintaining a human patient’s behavior and/or psychiatric state n the medicament is ated for periodic oral administration to the patient such that a dose of 90-225 mg of pridopidine is to be administered to the patient per day.

Provided herein is a pharmaceutical composition comprising pridopidine for use in reducing or maintaining a human patient’s involuntary movements wherein the pharmaceutical composition is to be periodically orally administered to the t such that a dose of 90-225 mg of pridopidine is to be administered to the patient per day.

Provided herein is use of an amount of pridopidine in the manufacture of a medicament for use in reducing or maintaining a human patient’s involuntary movements wherein the medicament is formulated for periodic oral administration to the patient such that a dose of 90-225 mg of pridopidine is to be administered to the patient per day.

Provided herein is a pharmaceutical composition comprising pridopidine for use in improving or maintaining a human t’s mobility wherein the ceutical composition is to be periodically orally administered to the patient such that a dose of 90-225 mg of pridopidine is to be administered to the patient per day.

Provided herein is use of an amount of pridopidine in the manufacture of a medicament for use in ing or maintaining a human patient’s mobility wherein the ment is formulated for periodic oral administration to the t such that a dose of 90-225 mg of idine is to be administered to the patient per day.

Provided herein is a pharmaceutical composition comprising pridopidine for use in improving or ining a human patient’s ability to perform physical tasks wherein the pharmaceutical composition is to be periodically orally administered to the patient such that a dose of 90-225 mg of pridopidine is to be stered to the patient per day.

Provided herein is a use of an amount of pridopidine in the manufacture of a medicament for use in improving or ining a human patient’s ability to perform physical tasks wherein the medicament is formulated for periodic oral administration to the patient such that a dose of 90-225 mg of pridopidine is to be administered to the patient per day.

The methods, compositions and uses disclosed herein are applicable, for example, to a human patient afflicted with Huntington’s e. In some embodiments of the methods compositions and uses, the human patient is afflicted with HD and has a ne TMS score which is in the least severe quarter of the overall population of patients ted with Huntington’s e; or the human patient is afflicted with HD and has a baseline TMS score which is in the two least severe quarters of the overall population of patients afflicted with Huntington’s disease; or the human t is ted with HD and has a baseline TMS score which is in the three least severe quarters of the overall population of patients afflicted with Huntington’s disease; or the human patient is afflicted with HD and has a baseline TMS score which is in the three least severe quarters of the overall population of patients afflicted with HD or a baseline TFC score which is greater than or equal to 9; or the human patient is afflicted with HD and has a baseline TMS score which is in the three least severe quarters of the overall population of patients afflicted with HD or a baseline TFC score which is greater than or equal to 9 or less than 44 CAG repeats in the Huntingtin gene; or the human patient is afflicted with HD and has a baseline TMS score which is in the two least severe quarters of the l population of patients afflicted with HD; or the human patient is afflicted with HD and has a baseline TFC score which is greater than or equal to 7; or the human patient is afflicted with HD and has a baseline TFC score of 11-13; or the human patient is afflicted with HD and has a baseline TFC score which is greater than or equal to 9 or greater than 44 CAG repeats in the huntingtin gene; or the human patient is afflicted with HD and has a baseline TMS score which is in the three least severe quarters of the overall population of ts afflicted with HD or less than 44 CAG repeats in the huntingtin gene; or the human patient is afflicted with HD and has a baseline TFC score which is greater than or equal to 9 or a ne TMS score which is in the three least severe rs of the l tion of patients afflicted with HD.

In some embodiments of the methods, compositions and uses disclosed herein the idine or a pharmaceutically acceptable salt thereof is idine hydrochloride.

A pharmaceutical composition comprising pridopidine or a pharmaceutically acceptable salt thereof, for e pridopidine hydrochloride, is to be periodically orally administered to the patient such that a dose of 90-225 mg of pridopidine is to be administered to the patient per day.

In some embodiments of the compositions and uses disclosed above, a dose of 90 mg or 180 mg of pridopidine is to be administered to the patient per day. In some ments of the methods disclosed above, a dose of 90mg of pridopidine is to be administered to the patient per day. In preferred embodiments of the methods disclosed above, the dose of 90 mg of pridopidine to be administered to the patient per day is to be administered to the patient as 45 mg bid.

In some embodiments, the patient is to be administered 45 mg pridopidine once daily (qd) for about one to two weeks and 45 mg pridopidine bid thereafter. In some embodiments of the methods disclosed above, the administration continues for at least 12 weeks, at least 26 weeks, at least 52 weeks or at least 78 weeks. In some embodiments of the methods disclosed above, the administration ues for 52 weeks or 78 weeks. In some embodiments of the methods disclosed above, the HD patient is a stage 1 or stage 2 HD patient and has a baseline TFC score of at least 7, at least 8, at least 9, at least 10, at least 11, at least 12, 13, or 7-10 or 11-13. In some embodiments of the methods disclosed above, the HD patient has been diagnosed as having at least 36 CAG repeats in the huntingtin gene. In some embodiments of the methods disclosed above the HD patient is 21 years old or older.

In some embodiments of the methods, compositions and uses disclosed above, the HD patient is a HD1 or HD2 patient and is not a pre-manifest HD patient.

BRIEF DESCRIPTION OF THE SEVERAL VIEWS OF THE DRAWINGS In the following brief descriptions of the figures and the corresponding figures, efficacy was assessed throughout the 52-week period using Mixed Models Repeated Measures (MMRM) analyses of change from baseline (prior to administration of pridopidine at week 0) in the UHDRS-TMS, UHDRS- Behavioral, UHDRS-Cognitive, TFC, Functional Assessment, UHDRS-Independence Scale, the modified Physical Performance Test (mPPT), individual TMS les, HD-Cognitive Assessment y (HD-CAB), Problem Behavior Assessment Short-Form (PBA-s), and other outcomes.

Figure 1: Pridopidine concentration in patient’s blood (ng/mL; Mean (+/-sd) measured values). “Pre” means predose and “post” mean post dose. V2 means visit 2, V3 means visit 3, etc. Wk2 means second week, Wk3 means third week, etc.

Figure 2: Pridopidine concentration in patient’s blood (ng/mL). ose (“Cmax”) ) at Steady State.

For Figures 1 and 2, a % coefficient variation (CV) of around 40% for measured values is considered te for this setting [1-2 hours post dose, patient population, sparse sampling]. Variability is expected to decrease once true sampling times are taken into consideration.

Figure 3: Total Motor Score (TMS) Change from Baseline (BL) with pridopidine administration.

The 90mg bid dose (circles) demonstrated the largest treatment effect. A se in TMS tes an improvement. Table 1 below shows the P-Values corresponding to Figure 3.

Table 1 Week 45mg bid 67.5mg bid 90mg bid 112.5mg bid 4 0.0304 0.0004 <.0001 <.0001 8 <.0001 <.0001 <.0001 <.0001 12 0.0002 0.0003 <.0001 0.0002 16 <.0001 <.0001 <.0001 <.0001 <.0001 <.0001 <.0001 <.0001 26 0.0013 0.0024 <.0001 0.0063 Figure 4: Total Motor Score (TMS) – Change from Baseline PRIDE-HD placebo vs historical placebo in HART and MermaiHD clinical . A lower number indicates improvement. There is about a 6.5 TMS point difference at week 26.

Figures 5a and 5b: Change from ne in TMS. Figure 5a: Using historical placebo in HART and MermaiHD clinical trials, TMS (change from baseline) results are significant for both 45mg pridopidine bid and 90mg pridopidine bid. A lower number indicates ement. Figure 5b: Change from baseline UHDRS-TMS full analysis set plotted over time. PRIDE-HD replicates previous data in TMS changes from baseline as the change from baseline values were similar to those in HART and MermaiHD. In this graph, a decrease in TMS change from baseline indicates improvement. Dark line with diamonds represents o, line with open circles represents 45mg bid, gray line with triangles represents 67.5mg bid, gray line with diamonds represents 90mg bid, line with squares represents 112.5mg bid. The 90mg bid dose demonstrated the largest treatment effect.

Figures 6a, 6b and 6c: Total Dystonia at week 12 (6a); at week 20 (6b); and at week 26 (6c) in patient groups administered ent doses of pridopidine. Y-axis is change in ia from baseline. All data refer to adjusted means +SE of change in dystonia in full analysis set. A lower number indicates improvement.

Figures 6d-6h show data relating to various aspects of dystonia.

Figure parison of ts with baseline (BL) dystonia score of >4 at 52 weeks after dosage with either placebo, 45 mg pridopidine b.i.d, 67.5 mg pridopidine , 90 mg pridopidine b.i.d., or 112.5 mg pridopidine b.i.d. Within the full analysis set, no clinically meaningful changes from baseline were noted for patients at Week 26 or Week 52 in the dystonia score across the placebo and all active treatment groups (not shown). In patients with a baseline total ia score greater than or equal to 4 assessed at Week 52, a directional clinical improvement in dystonia was noted for all treatment groups, with the greatest decreases ed for the 45, 67.5, and 90 mg bid treatment groups. The table below shows change from baseline in UHDRS dystonia score over time. week 4 8 12 16 20 26 52 Placebo n= 115 90 111 38 37 83 33 Pridop n= 109 82 102 25 24 81 21 ∆ to placebo -0.35 -0.24 -0.96 -0.35 -1.09 -1.01 -1.54 p value 0.3414 0.5783 0.0232 0.5515 0.0722 0.0326 0.0571 Figures 6e-6f: Black columns refer to responders: subjects with ement or no change in UHDRS dystonia score. Gray columns refer to sponders: subjects who exhibited a worsening in the UHDRS dystonia score. The number in the base of each column refers to the number of subjects.

Improvement or no change is reflected in a score of greater than or equal to 0, respectively.

Figure 6e: Percentage of subjects with UHDRS TMS dystonia (≥0) receiving either placebo or 45 mg pridopidine b.i.d. that were either responders or non-responders. Of those patients with baseline (BL) dystonia score of ≥4 who completed 52 weeks of ent with either placebo or 45 mg pridopidine b.i.d., the percentage who were categorized based on the change in UHDRS TMS ia from BL to 52 weeks as responders (improved or no change, e.g. change ≥0) or non-responders (worsened, change < 0).

Figure 6f: Of those patients with baseline (BL) dystonia score of ≥4 who completed 52 weeks of treatment with either placebo or 45 mg pridopidine b.i.d., the tage who were categorized based on the change in UHDRS TMS dystonia from BL to 52 weeks as responders (improved, e.g. change ≥1) or non-responders (worsened or no change < 1). Results of the der Analysis for dystonia items further support this trend toward improvement by showing that a greater percentage of patients were categorized as Responders within the dystonia items in the 45 mg bid treatment group compared to the placebo group (14 patients [77.8%] and 18 ts [60.0%], respectively) and the chorea + dystonia items in the 45 mg bid treatment group ed to the placebo group (14 patients [77.8%] and 20 patients ], respectively) (not shown).

Figure 6g: Plot of change in UHDRS Dystonia score over time for subjects pooled from MermaiHD, HART and HD studies with baseline (BL) dystonia (≥4) who received either placebo or 45 mg pridopidine b.i.d. At Week 26, patients taking 45 mg pridopidine b.i.d showed a statistically significant improvement in the dystonia score compared to those taking placebo. A trend toward this improvement was maintained at Week 52.

Figure 6h: Of those PRIDE-HD patients with baseline (BL) dystonia score of ≥4 who completed 52 weeks of treatment with either placebo or 45 mg idine , the tage who were categorized based on the change in UHDRS limb dystonia from BL to 52 weeks as responders (improved, e.g. change ≥1) or non-responders (worsened or no change < 1).

A tically significant greater percentage of patients were categorized as Responders for the UHDRS-Limb Dystonia item in the idine 45 mg bid treatment group compared to the placebo group (77.2% and 36.7%, respectively). Figure 7a: Change in dystonia in limbs (UHDRS -dystonia limbs) at week 12; Figure 7b: Finger Taps and Pronate-Supinate (P/S) hands at week 20; Figure 7c: Finger Taps and P/S hands at week 26. Finger Taps and e-Supinate (P/S) hands is a combination of finger tapping (the ability to tap the fingers of both hands where 15 repetitions in 5 seconds is considered normal) with pronation/supination (the ability to rotate the forearm and hand such that the palm is down (pronation) and to rotate the forearm and hand such that the palm is up (supination) on both sides of the body). Pronate-Supinate Hands is also known as the “Q-Motor: Pro- Sup-Frequency-MN-Hand (Hz)” .All data show to adjusted means +SE of change in dystonia in full analysis set for Figures 7a-7c.In the tables below, data and the P-Values corresponding to the figures are provided. N refers to number of patients. Wk26 refers to nt score at week 26. Wk52 refers to relevant score at week 52. "∆ to placebo" refers to the difference in score compared to placebo, specifically, the average change from baseline in the placebo group compared to the average change from baseline of the relevant group. "ALL" refers to pridopidine treated patients irrespective of disease stage. Y-axes are change from baseline for teristic listed above the table. X-axes are dose whereby P means “placebo”, 45 means “45mg bid,” 67.5 means “67.5 mg bid,” 90 means “90mg bid,” and 112.5 means “112.5 mg bid.” In the figures, improvement is in the direction from bottom of the graph to top of the graph.

For example, figure 8b shows the average difference in the UHDRS TMS score of the indicated group of patients (i.e. patients having a TFC score of 11-13 at ne, i.e. HD1) between the score at ne and the score after 26 weeks of administration of pridopidine (at week 26). In this figure, the 90mg bid dose shows the greatest improvement e its data point is the top most data point in the figure, showing an approximately 8 point improvement ed to baseline (i.e. a -8 UHDRS TMS score at week 26 compared to baseline). The table below the description of figure 8b shows that the 90mg bid group had 11 patients (“N” row) and an average UHDRS TMS score of 39.1 at baseline (“Baseline” row). The table below the description of figure 8b also shows that the 90mg bid group’s change from baseline (about -8, shown in figure, not shown in table) is 6.15 points better (-6.15) than the placebo group’s change from placebo (about -2, shown in figure, not shown in table)(“∆ to placebo” row). Additionally, the table below the description of figure 8b shows a p value of 0.0361 for the 90mg bid group (“p value” row). HD1 refers to an early stage HD patient with a ne TFC score of 11-13. HD2 refers to an early stage HD patient with a baseline TFC score of 7-10.

Figure 8a: Change from baseline in UHDRS TMS Week 26 ALL The table below and figure 8a show no significant improvement in UHDRS TMS in all pridopidine treated patients at 26 weeks compared to placebo. ement is evidenced by a more ve value in the UHDRS TMS score.

Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 81 75 79 81 81 Baseline 46.9 44.5 46.9 47 46.7 ∆ to placebo 1.42 1.71 0.67 2.1 p value 0.3199 0.2235 0.6282 0.1337 Figure 8b: Change from baseline in UHDRS TMS Week 26 Stage 1 BL TFC 11-13. (The UHDRS TMS score at week 26 of pridopidine treated patients with a baseline Total onal Capacity (BL TFC) score of 11 to 13). HD patients with a baseline TFC score of 11-13 are generally considered to be first stage (stage 1) HD patients. The table below and figure 8b show trend towards improvement in UHDRS TMS in HD1 pridopidine treated patients at 26 weeks compared to placebo.

Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 12 17 17 11 18 Baseline 37.3 35.4 36.4 39.1 38.7 25 ∆ to placebo -4.47 -3 -6.15 -4.79 p value 0.0976 0.2505 0.0361 0.0676 Figure 8c: Change from ne in UHDRS TMS Week 52 ALL. The table below and figure 8c show no significant improvement in UHDRS TMS in all pridopidine treated patients at 52 weeks, compared to placebo.

Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 81 75 79 81 81 Baseline 46.9 44.5 46.9 47 46.7 ∆ to placebo 0.59 2.55 1.78 2.71 p value 0.7468 0.1591 0.3144 0.137 Figure 8d: Change from ne in UHDRS TMS Week 52 Stage 1 BL TFC 11-13. The table below and figure 8d show a trend towards improvement in UHDRS TMS in HD1 idine treated ts at 52 weeks.

Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 12 17 17 11 18 Baseline 37.3 35.4 36.4 39.1 38.7 Wk52 ∆ to placebo -5.32 -0.84 -7.1 -0.92 p value 0.1065 0.7918 0.047 0.7765 Figure 8e: Change from baseline in UHDRS TMS Gait and Balances Week 52. The table below and figure 8e show no significant improvement in UHDRS TMS gait and balances in all pridopidine treated patients at 52 weeks.

Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 81 75 79 81 81 Baseline 3.8 4.1 4.1 4 3.8 ∆ to placebo -0.09 -0.05 -0.01 0.04 p value 0.7404 0.8532 0.9747 0.8923 Figure 8f: Change from baseline in UHDRS TMS Gait and Balances Week 52 Stage 1 BL TFC 11-13. The table below and figure 8f show a trend towards improvement in UHDRS TMS gait and balances in HD1 pridopidine treated patients at 52 weeks with significance for patients receiving 45 mg bid pridopidine.

Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 12 17 17 11 18 Baseline 2.3 2.8 2.6 2.6 2.4 ∆ to placebo -0.94 -0.53 -0.49 -0.4 p value 0.0445 0.2294 0.3056 0.3797 Figure 8g: Change from baseline in UHDRS TMS Chorea Week 26 ALL. The table below and figure 8g show no significant improvement in UHDRS TMS chorea in all pridopidine treated patients at 26 weeks.

Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 81 75 79 81 81 Baseline 11.4 10.9 11 11.2 10.9 ∆ to placebo 0.92 0.81 0.36 1.05 p value 0.1083 0.1501 0.5185 0.0609 Figure 8h: Change from baseline in UHDRS TMS Chorea Week 26 Stage 1 BL TFC 11-13. The table below and figure 8h show a trend towards ement in UHDRS TMS chorea in HD1 pridopidine treated patients at 26 weeks with significance for patients receiving 90mg bid pridopidine. 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 17 17 11 18 Wk26 ∆ to placebo -1.4 -2.07 -2.52 -1.08 p value 0.1805 0.0438 0.0271 0.2932 Figure 8i: Change from baseline in UHDRS TMS Dystonia Week 26 ALL. The table below and figure 8i show a trend towards improvement in UHDRS TMS dystonia in all pridopidine treated patients at 26 weeks.

Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 81 75 79 81 81 Baseline 4.1 3.6 4.1 4.9 4.5 ∆ to placebo -0.06 -0.34 -0.33 -0.29 p value 0.8711 0.3778 0.3845 0.4507 Figure 8j Change from ne in UHDRS TMS Dystonia Week 26 Stage 1 BL TFC 11-13. The table below and figure 8j show a trend towards improvement in UHDRS TMS dystonia in HD1 pridopidine treated patients at 26 weeks with significance for patients receiving 90mg bid pridopidine.

Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 12 17 17 11 18 Baseline 2.8 2.1 2.2 3.2 2.4 ∆ to placebo -0.99 -0.89 -1.56 -0.53 p value 0.1569 0.1882 0.0396 0.4303 Figure 8k: Change from baseline in UHDRS TMS Dystonia Week 52 The table below and figure 8k show a trend toward ement in UHDRS TMS dystonia in all pridopidine treated patients at 52 weeks.

Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 81 75 79 81 81 Baseline 4.1 3.6 4.1 4.9 4.5 ∆ to placebo -0.39 -0.35 -0.27 -0.24 p value 0.4358 0.4795 0.5858 0.6382 Figure 8l: Change from ne in UHDRS TMS Dystonia Week 52 Stage 1 BL TFC 11-13 The table below and figure 8l show a trend s improvement in UHDRS TMS dystonia in HD1 pridopidine treated patients at 52 weeks with icance for patients receiving 45mg bid pridopidine.

Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 12 17 17 11 18 Baseline 2.8 2.1 2.2 3.2 2.4 ∆ to placebo -1.65 -0.1 -1.46 -0.46 p value 0.0243 0.8848 0.0575 0.5228 Figure 8m: Change from baseline in UHDRS TMS Involuntary Movements Week 26 ALL. The table below and figure 8m show no significant improvement in UHDRS TMS ntary Movements in all pridopidine treated patients at 26 weeks.

Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 81 75 79 81 81 Baseline 15.6 14.4 15.1 16 15.4 ∆ to placebo 0.89 0.48 0.01 0.76 p value 0.2594 0.5328 0.9873 0.3268 Figure 8n: Change from baseline in UHDRS TMS Involuntary nts Week 26 Stage 1 BL TFC 11-13. The table below and figure 8n show significant improvement in UHDRS TMS Involuntary Movements at 26 weeks in HD1 idine treated patients receiving 45mg bid, 67.5 bid and 90 mg bid pridopidine.

Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 12 17 17 11 18 Baseline 11.5 12 12.2 12.9 13.2 ∆ to placebo -2.49 -3.07 -4 -1.64 p value 0.0469 0.0117 0.0033 0.1731 Figure 8o: Change from baseline in UHDRS TMS Involuntary Movements Week 52. The table below and figure 8o show no significant improvement in UHDRS TMS ntary Movements in all pridopidine treated patients at 52 weeks.

Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 81 75 79 81 81 Baseline 15.6 14.4 15.1 16 15.4 ∆ to placebo 0.02 0.8 -0.26 0.57 p value 0.9867 0.4196 0.7893 0.5648 Figure 8p: Change from baseline in UHDRS TMS Involuntary Movements Week 52 Stage 1 BL TFC 11-13. The table below and figure 8p show a trend s improvement in UHDRS TMS ntary Movements in HD1 pridopidine treated patients at 52 weeks, in particular in 45 mg bid and 90 mg bid treated patients.

Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 12 17 17 11 18 Baseline 11.5 12 12.2 12.9 13.2 ∆ to placebo -2.73 -0.2 -3.8 0.8 p value 0.1487 0.9111 0.0643 0.6751 Figure 8q: Change from baseline in UHDRS TMS Excluding Chorea Week 52. The table below and figure 8q show no significant improvement in UHDRS TMS excluding chorea in all pridopidine treated patients at 52 weeks.

Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 81 75 79 81 81 Baseline 35.5 33.6 35.9 35.8 35.8 ∆ to placebo 0.05 1.31 1.67 1.94 p value 0.9693 0.3495 0.2234 0.1704 Figure 8r: Change from baseline in UHDRS TMS Excluding Chorea Week 52 Stage 1 BL TFC 11-13. The table below and figure 8r show a trend towards ement in UHDRS TMS excluding chorea in HD1 pridopidine treated patients at 52 weeks, in particular in the 45 mg bid and 90 mg bid treated patients.

Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 12 17 17 11 18 Baseline 28.6 25.5 26.4 29.4 27.8 ∆ to placebo -4.09 -0.18 -4.92 -1.59 p value 0.083 0.9358 0.0505 0.4924 Figure 8s: Change from baseline in UHDRS TMS Excluding Dystonia Week 26 ALL. The table below and figure 8s show no significant improvement in UHDRS TMS excluding dystonia in all pridopidine treated ts at 26 weeks.

Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 81 75 79 81 81 Baseline 42.7 40.9 42.8 42.1 42.2 ∆ to placebo 1.39 1.97 1.2 2.4 p value 0.2733 0.1137 0.3314 0.0539 Figure 8t: Change from baseline in UHDRS TMS Excluding Dystonia Week 26 Stage 1 BL TFC 11-13. The table below and figure 8t show a trend towards improvement in UHDRS TMS excluding dystonia in HD1 idine treated patients, at 26 weeks.

Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 12 17 17 11 18 Baseline 34.6 33.4 34.1 35.9 36.3 ∆ to placebo -3.6 -2.2 -4.35 -4.31 p value 0.1594 0.376 0.1167 0.0842 Figure 9a: Change from baseline in UHDRS Total Functional ment Week 26 ALL. The table below and figure 9a show no significant improvement in UHDRS TFC in all pridopidine treated patients at 26 weeks. Improvement is ced by a higher TFC score.

Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 81 75 79 81 81 Baseline 18.6 19 18.6 18.8 19.1 ∆ to placebo 0.02 0.09 -0.41 -0.1 p value 0.9511 0.8211 0.277 0.7979 Figure 9b: Change from baseline in UHDRS Total Functional Assessment Week 26 Stage 1 BL TFC 11-13. The table below and figure 9b show a trend towards improvement in UHDRS TFC in HD1 pridopidine treated patients, at 52 weeks, in particular in the 45 mg bid treated ts.

Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 12 17 17 11 18 Baseline 22.8 23.9 23 23.1 22.9 ∆ to placebo 1.23 1.08 0.87 1.33 p value 0.0516 0.0696 0.1899 0.0273 Figure 9c: Change from baseline in UHDRS Independence Scale Week 26 ALL. The table below and figure 9c show significant improvement in UHDRS IS in all 45 mg pridopidine treated patients at 26 weeks.

Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 81 75 79 81 81 Baseline 76.4 76.1 74.6 76.3 75.6 ∆ to placebo 1.79 0.3 0.78 1.41 p value 0.0328 0.7124 0.341 0.0887 Figure 9d: Change from baseline in UHDRS Independence Scale Week 26 Stage 1 BL TFC 11- 13. The table below and figure 9d show improvement in UHDRS IS in 45 mg bid d HD1 patients, after 26 weeks.

Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 12 17 17 11 18 Baseline 83.8 84.1 81.5 84.1 83.1 ∆ to placebo 4.94 2.27 1.73 2.38 p value 0.001 0.1126 0.2738 0.0958 Figure 9e: Change from ne in 9e UHDRS Independence Scale Week 52 ALL. The table below and figure 9e show no significant improvement in UHDRS IS in all ts treated patients after 52 weeks.

Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 81 75 79 81 81 Baseline 76.4 76.1 74.6 76.3 75.6 ∆ to placebo 0.86 0.25 -0.07 0.18 p value 0.5082 0.8431 0.9558 0.8871 Figure 9f: Change from baseline in UHDRS Independence Scale Week 52 Stage 1 BL TFC 11- 13. The table below and figure 9f show a trend towards improvement in UHDRS IS in 45 mg bid treated HD1 patients, after 52 weeks.

Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 12 17 17 11 18 Baseline 83.8 84.1 81.5 84.1 83.1 ∆ to placebo 3.05 0.91 1.16 -1.61 p value 0.1289 0.6415 0.5899 0.4193 Figure 9g: Domestic Chores at 52 weeks, Early Stage HD (TFC>7). The table below provides data and the P-Values ponding to Figure 9g. icant improvement in TFC ic chores was observed in 45 mg bid pridopidine administered HD1 and HD2 ts, for 52 weeks.

Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 62 59 54 56 58 Baseline 1.4 1.5 1.4 1.4 1.4 ∆ to placebo 0.24 0.09 0.16 0.04 p value 0.0196 0.3829 0.1155 0.7145 Figure 9h: Care Level at 52 weeks, Early Stage HD (TFC>7). The table below provides data and the P-Values corresponding to Figure 9h. Significant improvement in TFC Care level was observed in 45 mg bid to 90 mg bid pridopidine administered HD1 and HD2 patients for 52 weeks.

Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 62 59 54 56 58 ne 2 1.9 2 2 2 ∆ to placebo 0.12 0.09 0.08 0.04 p value 0.0044 0.0319 0.0411 0.403 Figure 10a: Change from baseline in UHDRS Total Functional Capacity Week 26 ALL. The table below and figure 10a show a trend toward improvement in UHDRS TFC in all pridopidine treated patients after 26 weeks.

Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 81 75 79 81 81 Baseline 7.9 8.1 7.8 7.8 8 ∆ to placebo 0.34 0.21 0.33 0.42 p value 0.1474 0.3639 0.1465 0.0676 Figure 10b: Change from baseline in UHDRS Total Functional Capacity Week 26 Stage 1 BL TFC 11-13. The table below and figure 10b show ement in UHDRS IS in 45 mg bid and 90 mg bid HD1 pridopidine treated patients, for 26 weeks.

Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 12 17 17 11 18 Baseline 11.8 11.5 11.5 11.7 11.8 ∆ to placebo 1.65 0.84 1.43 1.75 p value 0.004 0.1245 0.0191 0.0019 Figure 10c: Change from baseline in UHDRS Total Functional Capacity Week 52. The table below and figure 10c show reduction in functional decline as measured by TFC score in all patients receiving 45mg bid pridopidine for 52 weeks.

Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 81 75 79 81 81 Baseline 7.9 8.1 7.8 7.8 8 ∆ to placebo 0.87 0.11 0.19 0.24 p value 0.0032 0.7042 0.5099 0.4061 Figure 10d: Change from baseline in UHDRS Total Functional Capacity Week 52 Stage 1 BL TFC 11-13. The table below and figure 10d show statistically significant reduced functional decline as measured by TFC in HD1 patient, receiving 45mg bid pridopidine for 52 weeks.

Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 12 17 17 11 18 Baseline 11.8 11.5 11.5 11.7 11.8 ∆ to placebo 1.89 -0.03 0.99 1.06 p value 0.0059 0.9588 0.1678 0.1154 Figure 10e: Change from baseline in UHDRS Total Functional Capacity Week 52 Stage 2 BL TFC 7-10. The table below and figure 10e show statistically significant reduced functional decline as measured by TFC in HD2 patients receiving 45mg bid idine for 52 weeks.

Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 50 42 37 45 40 Baseline 8.3 8.2 8.4 8.5 8.2 ∆ to placebo 0.94 0.64 0.51 0.03 p value 0.009 0.0924 0.1448 0.9331 Figure 11a: Change from baseline in UHDRS TFC Finance ADL Week 26 ALL. The table below and figure 11a show a trend towards improvement in ADL finance as measured as part of the UHDRS TFC score in all patients receiving pridopidine for 26 weeks.

Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 81 75 79 81 81 Baseline 4 4.1 3.9 4 4 ∆ to placebo 0.22 0.16 0.31 0.38 p value 0.1782 0.3184 0.0543 0.0168 Figure 11b: Change from baseline in UHDRS TFC Finance ADL Week 26 Stage 1 BL TFC 11- 13. The table below and figure 11b show statistically significant ement in ADL finances as measured as part of the TFC score in HD1 patients ing all doses pridopidine for 26 weeks.

Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 12 17 17 11 18 Baseline 5.8 5.7 5.8 5.9 5.9 ∆ to placebo 0.92 0.65 0.97 1.12 p value 0.0012 0.0168 0.0017 <.0001 Figure 11c: Change from baseline in UHDRS TFC Finance ADL Week 26 Stage 2 BL TFC 7-10.

The table below and figure 11c show tically significant improvement in ADL finances as measured as part of the TFC score in HD2 patients, receiving highest dose pridopidine for 26 weeks.

Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 50 42 37 45 40 ne 4.4 4.3 4.4 4.5 4.2 ∆ to placebo 0.33 0.26 0.3 0.46 p value 0.1492 0.2634 0.1674 0.0459 Figure 11d: Change from baseline in UHDRS TFC Finance ADL Week 52 ALL. The table below and figure 11d show a statistically significant improvement in ADL finance as measured as part of the UHDRS TFC score in all ts receiving 45 mg bid pridopidine for 52 weeks.

Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 81 75 79 81 81 Baseline 4 4.1 3.9 4 4 ∆ to placebo 0.46 0.1 0.17 0.1 p value 0.0164 0.5831 0.3558 0.6018 Figure 11e: Change from baseline in UHDRS TFC Finance ADL Week 52 Stage 1 BL TFC 11- 13. The table below and figure 11e show statistically significant improvement in ADL finances as measured as part of the TFC score in HD1 patients, receiving 45 mg bid pridopidine for 26 weeks.

Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 12 17 17 11 18 ne 5.8 5.7 5.8 5.9 5.9 ∆ to placebo 0.77 -0.18 0.4 0.64 p value 0.0277 0.5997 0.2805 0.0697 Figure 11f: Change from baseline in UHDRS TFC Finance ADL Week 26 Stage 2 BL TFC 7-10.

The table below and figure 11f show statistically significant improvement in ADL es as measured as part of the TFC score in HD2 patients, receiving 45 -90 mg bid pridopidine for 26 weeks.

Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 50 42 37 45 40 Baseline 4.4 4.3 4.4 4.5 4.2 ∆ to placebo 0.7 0.54 0.56 0.18 p value 0.0045 0.0407 0.0199 0.4962 Figure 12a: Change from baseline in UHDRS TFC Finances Week 26 ALL. The table below and figure 12a show no significant improvement in UHDRS TFC finances in all idine treated patients at 26 weeks.

Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 81 75 79 81 81 Baseline 1.6 1.8 1.7 1.7 1.7 ∆ to placebo 0.1 0.05 0.15 0.21 p value 0.3629 0.6131 0.1389 0.0449 Figure 12b: Change from baseline in UHDRS TFC es Week 26 Stage 1 BL TFC 11-13.

The table below and figure 12b show statistically significant improvement in UHDRS TFC finances in HD1 patients, receiving > 67.5 mg bid idine for 26 weeks.

Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 12 17 17 11 18 Baseline 2.8 2.9 2.8 2.9 2.9 ∆ to placebo 0.25 0.31 0.43 0.44 p value 0.1183 0.0494 0.0162 0.0062 Figure 12c: Change from ne in UHDRS TFC Finances Week 52. The table below and figure 12c show statistically significant improvement in TFC finances in HD1 patients, receiving 45 mg bid pridopidine for 52 weeks.

Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 81 75 79 81 81 Baseline 1.6 1.8 1.7 1.7 1.7 ∆ to placebo 0.31 0.05 0.16 0.05 p value 0.0143 0.6644 0.1976 0.7059 Figure 12d: Change from baseline in UHDRS TFC Finances Week 52 Stage 2 BL TFC 7-10. The table below and figure 12b show statistically significant ement in UHDRS TFC finances in HD2 patients, receiving 45 and 90 mg bid pridopidine for 26 weeks. o 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 50 42 37 45 40 ne 1.8 1.9 1.9 1.9 1.8 ∆ to placebo 0.39 0.23 0.4 0.01 p value 0.0336 0.24 0.0248 0.9559 Figure 13a: Change from baseline in UHDRS TFC Domestic Chores Week 26 ALL. The table below and figure 13a show no significant improvement in UHDRS TFC domestic chores in all pridopidine treated patients at 26 weeks.

Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 81 75 79 81 81 Baseline 1.2 1.3 1.3 1.2 1.2 ∆ to placebo -0.01 0.02 0 0.06 p value 0.9015 0.8331 0.977 0.438 Figure 13b: Change from baseline in UHDRS TFC Domestic Chores Week 26 Stage 1 BL TFC 11-13. The table below and figure 13b show a trend towards improvement in TFC domestic chores in HD1 patients receiving pridopidine for 26 weeks.

Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 12 17 17 11 18 Baseline 2 1.8 1.9 1.7 1.9 ∆ to placebo 0.34 0.21 0.34 0.47 p value 0.0589 0.2169 0.0872 0.0079 Figure 13c: Change from baseline in UHDRS TFC ic Chores Week 52 ALL. The table below and figure 13c show no significant improvement in UHDRS TFC domestic chores in all pridopidine treated patients at 52 weeks.

Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 59 56 59 62 67 Baseline 1.3 1.3 1.3 1.2 1.2 ∆ to placebo 0.23 -0.03 0.05 0.04 p value 0.0647 0.7825 0.6869 0.7093 Figure 13d: Change from baseline in UHDRS TFC ic Chores Week 52 Stage 1 BL TFC 11-13. The table below and figure 13d show statistically icant improvement in TFC domestic chores in HD1 patients receiving 45 mg bid idine for 52 weeks. o 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 12 17 17 11 18 Baseline 2 1.8 1.9 1.7 1.9 ∆ to placebo 0.49 0.05 0.1 0.23 p value 0.0161 0.7793 0.6442 0.2463 Figure 14a: Change from baseline in UHDRS TFC ADL Week 26 ALL. The table below and figure 14a show no significant improvement in TFC ADL in all pridopidine treated patients at 26 weeks.

Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 81 75 79 81 81 Baseline 2.4 2.3 2.2 2.3 2.3 ∆ to placebo 0.12 0.09 0.14 0.17 p value 0.205 0.3427 0.1296 0.0773 Figure 14b: Change from baseline in UHDRS TFC ADL Week 26 Stage 1 BL TFC 11-13. The table below and figure 14b show statistically significant improvement in UHDRS TFC ADL in HD1 patients receiving 45 mg bid, 90 mg bid and 112.5 mg bid pridopidine for 26 weeks.

Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 12 17 17 11 18 Baseline 2.9 2.8 2.9 3 3 ∆ to placebo 0.65 0.34 0.58 0.7 p value 0.0011 0.0715 0.0062 0.0003 Figure 14c: Change from baseline in UHDRS TFC ADL Week 52 ALL. The table below and figure 14c show no significant improvement in UHDRS TFC ADL in all pridopidine treated patients at 52 weeks.

Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 81 75 79 81 81 Baseline 2.4 2.3 2.2 2.3 2.3 ∆ to placebo 0.14 0.03 -0.01 0.03 p value 0.2216 0.7943 0.9318 0.7868 Figure 14d: Change from baseline in UHDRS TFC ADL Week 52 Stage 1 BL TFC 11-13. The table below and figure 14d show statistically significant improvement in UHDRS TFC ADL in HD1 patients receiving 45 mg bid or 112.5 mg bid pridopidine for 52 weeks. o 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 12 17 17 11 18 Baseline 2.9 2.8 2.9 3 3 ∆ to placebo 0.62 0.21 0.42 0.46 p value 0.0044 0.3054 0.0646 0.0345 Figure 14e: Change from baseline in UHDRS TFC ADL Week 52 Stage 2 BL TFC 7-10. The table below and figure 14e show statistically significant ement in UHDRS TFC ADL in HD2 patients ing 45 mg bid pridopidine for 52 weeks.

Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 50 42 37 45 40 Baseline 2.6 2.5 2.5 2.5 2.4 ∆ to placebo 0.27 0.31 0.16 0.15 p value 0.0356 0.0244 0.1894 0.2776 Figure 15a: Change from baseline in UHDRS TFC Care Level Week 52 ALL. The table below and figure 15a show no significant improvement in UHDRS TFC care level in all pridopidine treated patients at 52 weeks.

Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 59 56 59 62 67 Baseline 1.9 1.9 1.9 1.9 1.9 ∆ to placebo 0.09 0 -0.08 -0.03 p value 0.1153 0.9365 0.1509 0.5713 Figure 15b: Change from baseline in UHDRS TFC Care Level Week 52 Stage 2 BL TFC 7-10.

The table below and figure 15b show statistically significant improvement in UHDRS TFC care level in HD2 patients ing 45 mg bid idine for 52 weeks.

Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 50 42 37 45 40 Baseline 1.9 1.9 2 2 2 ∆ to placebo 0.13 0.12 0.1 0.03 p value 0.0156 0.0395 0.0585 0.6168 Figure 16a: Change from baseline in HD-QoL Participant Total Score Week 26 ALL. The table below and figure 16a show no significant improvement in HD-QoL in all pridopidine treated patients at 26 weeks.

Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 81 75 79 81 81 Baseline 68.1 67.3 76.5 73.3 69.9 ∆ to placebo 1.91 8.7 6.95 -1.36 p value 0.6775 0.0572 0.1251 0.7663 Figure 16b: Change from baseline in HD-QoL Participant Total Score Week 26 Stage 2 BL TFC 7-10. The table below and figure 16b show significant improvement in HD-QoL in 67.5 mg bid pridopidine treated HD2 patients at 26 weeks.

Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 50 42 37 45 40 Baseline 62.5 64.3 82.8 74.3 78 ∆ to placebo 3.22 16.33 10.64 4.29 p value 0.5601 0.0054 0.0566 0.4577 Figure 17a: Change from ne in PBA Total Score Week 26 ALL, full analysis set. The table below and figure 17a show change in baseline in PBA total score total in pridopidine treated patients at 52 weeks.

` Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 81 75 79 81 81 Baseline 12 10.9 13.8 11.2 11.8 ∆ to placebo -0.46 -1.83 0.51 -1.85 p value 0.7838 0.2748 0.7567 0.2659 Figure 17b: Change from baseline in PBA Total Score Week 26 Stage 1 BL TFC 11-13. The table below and figure 17b show a trend towards ement in PBA total score in HD1 patients receiving pridopidine for 26 weeks. o 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 12 17 17 11 18 Baseline 8.8 8.1 10.2 4 7.7 ∆ to o -4.83 -9.22 -4.74 -7.08 p value 0.319 0.0533 0.3721 0.1351 Figure 17c: Change from baseline in PBA Depressed Mood, Severity x Frequency Week 26 ALL.

The table below and figure 17c show no significant improvement in PBD depressed mood in all pridopidine treated patients at 26 weeks.

Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 81 75 79 81 81 Baseline 1.5 1.8 1.9 1.2 1.3 ∆ to placebo -0.29 -0.65 -0.34 -0.52 p value 0.4015 0.0583 0.3174 0.1237 Figure 17d: Change from baseline in PBA Depressed Mood, Severity x Frequency Week 26 Stage 1 BL TFC 11-13. The table below and figure 17d show no significant improvement in PBA depressed mood in HD1 pridopidine treated patients at 26 weeks.

Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 12 17 17 11 18 Baseline 1.9 1.3 2.2 0.8 1.1 ∆ to placebo -0.63 -2.01 -0.84 -1.43 p value 0.5782 0.0704 0.4957 0.1942 Figure 17e: PBA Change from baseline in Total Score Week 52 Full analysis set. The table below and figure 17e show trend to improvement in PBA total score in 45 mg bid pridopidine treated ts at 52 weeks.

N 81 75 79 81 81 Baseline 12 10.9 13.8 11.2 11.8 ∆ to placebo -3.98 -0.63 -0.38 0.3 p value 0.0603 0.7602 0.851 0.8845 Figure 17f: Change from baseline in PBA Total Score Week 52 Full is set BL TFC >7. The table below and figure 17f show trend to ement in PBA total score in 45 mg bid pridopidine treated HD1 and HD2 patients at 52 weeks.

Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 62 59 54 56 58 Baseline 11.4 10.1 14.4 10.9 10.4 ∆ to placebo -2.74 0.61 0.9 1.4 p value 0.1911 0.7785 0.6653 0.5171 Figure 17g: Change from baseline PBA Irritability, Severity x ncy Week 52 ALL. The table below and figure 17g show significant improvement in PBA irritability in most (excluding 67.5 mg bid) pridopidine treated patients at 52 weeks.

Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 81 75 79 81 81 Baseline 2 1.6 1.7 1.4 1.5 ∆ to placebo -1.03 -0.63 -1.01 -0.84 p value 0.0126 0.1176 0.0108 0.0419 Figure 17h: Change from baseline in PBA Irritability, Severity x Frequency Week 52 Stage 3-5 BL TFC 0-6. The table below and figure 17h show significant improvement in PBA irritability in pridopidine treated patients with baseline TFC 0-6 at 52 weeks. o 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 19 16 25 25 23 ne 1.3 0.9 0.9 1.3 1.6 ∆ to placebo -2.42 -1.78 -1.79 -1.71 p value 0.0165 0.0429 0.0422 0.0542 Figure 17i: Change from baseline in PBA Lack of Initiative (Apathy), Severity x Frequency Week 26 ALL. The table below and figure 17i show no significant improvement in PBA apathy in all pridopidine treated patients at 26 weeks.

Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 81 75 79 81 81 Baseline 2.6 2.5 3.1 2.9 3 ∆ to placebo -0.87 -0.53 -0.2 -0.26 p value 0.1235 0.3437 0.7198 0.6445 Figure 17j: Change from baseline in PBA Lack of Initiative (Apathy), Severity x Frequency Week 26 Stage 1 BL TFC 11-13. The table below and figure 17j show trend towards improvement in PBA apathy in HD1 patients receiving pridopidine for 26 weeks.

Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 12 17 17 11 18 ne 1.2 1 1.3 0.4 1.5 ∆ to placebo -1.85 -1.51 -1.46 -2.62 p value 0.0703 0.1267 0.1822 0.0089 Figure 17k: Change from baseline in PBA Lack of Initiative (Apathy), Severity x Frequency Week 52 Full is set. The table below and figure 17k show trend s improvement in PBA apathy in BL stage 1 ts receiving pridopidine for 52 weeks.

Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid Placebo N 81 75 79 81 81 Baseline 2.6 2.5 3.1 2.9 3 ∆ to placebo -1.27 0.26 -0.12 -0.04 p value 0.0704 0.7052 0.8599 0.9523 Figure 17l: PBA Change from baseline in PBA Lack of Initiative (Apathy), Severity x Frequency Week 52 1 BL TFC >7. The table below and figure 17l show trend towards improvement in PBA apathy in HD1 and HD2 pridopidine treated patients for 52weeks.

Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid Placebo N 62 59 54 56 58 Baseline 2.5 2 3 2.7 2.8 ∆ to o -1.39 -0.29 -0.02 0.26 p value 0.0608 0.703 0.9734 0.7346 Figure 17m: Change from baseline in PBA Obsessive-Compulsive, Severity x Frequency Week 26 ALL. The table below and figure 17m show no significant improvement in PBA O-C in all pridopidine treated patients at 26 weeks.

Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 81 75 79 81 81 Baseline 1.2 1.1 1.3 1.1 1 ∆ to placebo 0.1 -0.45 -0.12 -0.63 p value 0.8081 0.2512 0.7541 0.1061 Figure 17n Change from ne in PBA Obsessive-Compulsive, Severity x Frequency Week 26 Stage 1 BL TFC 11-13. The table below and figure 17n show statistically significant improvement in PBA O-C in HD1 patients receiving pridopidine for 26 weeks.

Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 12 17 17 11 18 Baseline 0 1 0.4 0.1 0.1 ∆ to placebo -2.11 -2.03 -1.71 -1.73 p value 0.0035 0.0035 0.0251 0.0114 Figure 17o: Change from baseline in PBA Obsessive-Compulsive, Severity x ncy Week 52 ALL. The table below and figure 17o show no significant improvement in PBA O-C in all pridopidine treated patients at 52 weeks.

Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 81 75 79 81 81 Baseline 1.2 1.1 1.3 1.1 1 ∆ to placebo -0.24 -0.28 -0.13 -0.12 p value 0.5733 0.5068 0.7508 0.7789 Figure 17p: Change from ne in PBA Obsessive-Compulsive, Severity x Frequency Week 52 Stage 1 BL TFC 11-13. The table below and figure 17p show statistically significant improvement in PBA O-C in HD1 patients receiving pridopidine for 52 weeks Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 12 17 17 11 18 Baseline 0 1 0.4 0.1 0.1 ∆ to placebo -2.73 -3.24 -2.47 -2.73 p value 0.007 0.0011 0.021 0.005 Figure 17q: Change from baseline in PBA Disoriented Behavior, Severity x ncy Week 26 ALL. The table below and figure 17q show no significant improvement in PBA Disoriented Behavior in all pridopidine treated patients at 26 weeks.

Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 81 75 79 81 81 Baseline 0.6 0.4 0.8 0.6 0.6 ∆ to placebo -0.2 -0.28 -0.09 -0.08 p value 0.2864 0.1357 0.607 0.6771 Figure 17r: Change from baseline in PBA Disoriented or, ty x Frequency Week 26 Stage 1 BL TFC 11-13. The table below and figure 17r show significant improvement in PBA Disoriented Behavior in HD1 patients receiving 45 mg bid or 112.5 mg bid pridopidine at 26 weeks.

Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 12 17 17 11 18 Baseline 0.1 0 0.3 0 0.3 ∆ to placebo -0.19 -0.16 -0.16 -0.18 p value 0.0381 0.0615 0.093 0.0357 Figure 18a: Change from baseline in Timed Up and Go Test (sec) Week 26 ALL. The table below and figure 18a show no icant improvement in Timed up and go test in all pridopidine treated patients at 26 weeks.

Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 81 75 79 81 81 Baseline 12.1 12.1 10.4 10.3 11.6 ∆ to placebo -2.16 -0.09 -1.54 -2.33 p value 0.1765 0.9571 0.3255 0.1456 Figure 18b: Change from baseline in Timed Up and Go Test (sec) Week 26 Stage 1 BL TFC 11- 13. The table below and figure 18b show a trend towards improvement in the Timed up and go test in pridopidine treated HD1 patients at 26 weeks.

Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 12 17 17 11 18 Baseline 9.7 8.6 8.7 9.6 9.4 ∆ to placebo -6.98 -5.59 -5.87 -7.24 p value 0.0612 0.1259 0.1498 0.0482 Figure 18c: Change from baseline in Timed Up and Go Test (sec) Week 52. The table below and figure 18c show no statistically significant improvement in the Timed up and go test in all pridopidine treated patients at 52 weeks.

Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 81 75 79 81 81 ne 12.1 12.1 10.4 10.3 11.6 ∆ to placebo -1.49 -0.74 0.22 -0.47 p value 0.0899 0.4022 0.7918 0.595 Figure 18d: Change from baseline in Timed Up and Go Test (sec) Week 52 Stage 1 BL TFC 11- 13. The table below and figure 18d show trend toward ement in the Timed up and go test in pridopidine treated HD1patients at 52 weeks.

Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 12 17 17 11 18 Baseline 9.7 8.6 8.7 9.6 9.4 ∆ to placebo -5.26 -4.65 -4.02 -5.13 p value 0.0627 0.0921 0.1859 0.0652 Figure 19a: Change from baseline in Walk-12 Total Score Week 26 ALL. The table below and figure 19a show no significant improvement in the Walk-12 TS in all pridopidine treated patients at 26 weeks.

Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 81 75 79 81 81 Baseline 31.5 32.1 32.8 29.7 29.7 ∆ to placebo -2.45 0.13 1.7 -4.71 p value 0.3359 0.9604 0.4931 0.0622 Figure 19b: Change from baseline in Walk-12 Total Score Week 26 Stage 1 BL TFC 11-13. The table below and figure 19b show statistically significant improvement in the Walk-12 TS in pridopidine treated HD1 patients having at 26 weeks.

Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 12 17 17 11 18 Baseline 21.2 6.3 12.3 17.7 13 ∆ to placebo -9.63 -7.45 -10.88 -9.38 p value 0.0241 0.054 0.0116 0.0173 Figure 19c: Change from baseline in Walk-12 Total Score Week 26 Stage 3-5 BL TFC 0-6 .The table below and figure 19c show no significant improvement in the Walk-12 TS in late stage idine treated ts (BL TFC 0-6) at 26 weeks.

Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 19 16 25 25 23 ne 56.6 55.4 48.3 39.6 45.9 ∆ to placebo -1.97 -4.7 -3.18 -14.22 p value 0.7524 0.4242 0.5934 0.0151 Figure 19d: Change from baseline in Walk-12 Total Score Week 52 ALL. The table below and figure 19d show no tically significant improvement in the Walk-12 TS in idine treated patients at 52weeks.

Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 81 75 79 81 81 Baseline 31.5 32.1 32.8 29.7 29.7 ∆ to placebo 1.62 3.01 2.53 0.56 p value 0.6486 0.3891 0.4587 0.8738 Figure 19e: Change from ne in Walk-12 Total Score Week 52 Stage 1 BL TFC 11-13. The table below and figure 19e show statistically significant improvement in the Walk-12 TS in 90 mg bid pridopidine treated HD1 patients at 52 weeks.

Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 12 17 17 11 18 Baseline 21.2 6.3 12.3 17.7 13 ∆ to placebo -5.86 -8.57 -13.6 -4.13 p value 0.3018 0.1032 0.0193 0.4534 Figure 20a: Change from baseline in UHDRS Independence Scale Week 26 BL TFC <7. The table below and figure 20a show no significant improvement in the UHDRS IS in pridopidine treated patients having BL TFC less than 7, at 26 weeks. o 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 19 16 25 25 23 Baseline 65.5 63.8 64.8 68.2 66.3 ∆ to placebo 0.3 -0.44 0.2 1.65 p value 0.8796 0.8027 0.9116 0.3578 Figure 20b: Change from baseline in UHDRS Independence Scale Week 26 BL TFC ≥7. The table below and figure 20b show statistically significant improvement in the UHDRS IS in 45 mg bid idine treated HD1 and HD2 patients, at 26 weeks. 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 59 54 56 58 Wk26 ∆ to placebo 2.22 0.99 1.48 1.51 p value 0.0128 0.2755 0.0949 0.0919 Figure 20c: Change from baseline in UHDRS Independence Scale Week 52 BL TFC <7. The table below and figure 20a show no significant improvement in the UHDRS IS in pridopidine treated patients having baseline TFC less than 7, at 52weeks.

Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 19 16 25 25 23 Baseline 65.5 63.8 64.8 68.2 66.3 ∆ to placebo -1.85 -3.46 -5.25 -0.52 p value 0.5799 0.2415 0.0779 0.8613 Figure 20d: Change from baseline in UHDRS Independence Scale Week 52 BL TFC ≥7. The table below and figure 20d show statistically significant improvement in the UHDRS IS in 90 mg bid pridopidine treated HD1 and HD2 patients at 52 weeks.

Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 62 59 54 56 58 Baseline 79.8 79.4 79.2 79.9 79.3 ∆ to placebo 1.99 2.22 2.79 0.44 p value 0.1047 0.0788 0.0228 0.7301 Figures p, 22a-22b, 23a-23b, 24a-24b are graphs ing characteristics in early stage (TFC>7, HD1 and HD2) or late stage (TFC<7) HD patients.

Figure 21a: Change from ne in UHDRS Total Functional Capacity Week 26 BL TFC <7.

The table below and figure 21a show no significant improvement in the UHDRS TFC in pridopidine treated late stage patients, at 26 weeks.

Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 62 59 54 56 58 Baseline 8.9 9.2 9.4 9.1 9.3 ∆ to placebo 0.56 0.33 0.61 0.67 p value 0.0359 0.215 0.0199 0.0125 Figure 21b: Change from baseline in UHDRS Total Functional Capacity Week 26 BL TFC ≥7.

The table below and figure 21b show statistically significant improvement in the UHDRS TFC in 45 mg bid and 90 mg bid and higher pridopidine treated HD1 and HD2 patients, at 26 weeks.

Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 62 59 54 56 58 Baseline 8.9 9.2 9.4 9.1 9.3 Wk26 ∆ to placebo 0.56 0.33 0.61 0.67 p value 0.0359 0.215 0.0199 0.0125 Figure 21c: Change from baseline in UHDRS TFC Finance ADL Week 26 BL TFC <7. The table below and figure 21c show no significant improvement in the UHDRS TFC Finance ADL in pridopidine treated late stage patients, at 26 weeks.

Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 19 16 25 25 23 Baseline 2 2 1.9 2.2 2.3 ∆ to o -0.34 -0.06 0.28 0.03 p value 0.3239 0.8408 0.3747 0.9361 Figure 21d: Change from baseline in UHDRS TFC e ADL Week 26 BL TFC ≥7. The table below and figure 21d show statistically significant improvement in the UHDRS e ADL in 45 mg bid and 90 mg bid and higher pridopidine treated HD1 and HD2 patients, at 26 weeks.

Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 62 59 54 56 58 Baseline 4.6 4.7 4.9 4.8 4.7 ∆ to placebo 0.46 0.32 0.47 0.62 p value 0.0114 0.0817 0.0093 0.0007 Figure 21e: Change from ne in UHDRS TFC Finances Week 26 BL TFC <7. The table below and figure 21e show no icant improvement in the UHDRS ITFC finances in pridopidine treated late stage patients, at 26 weeks.

Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 19 16 25 25 23 Baseline 0.5 0.5 0.5 0.7 0.7 ∆ to placebo -0.19 -0.1 0.05 0.03 p value 0.3508 0.5934 0.774 0.8925 Figure 21f: Change from baseline in UHDRS TFC es Week 26 BL TFC ≥7. The table below and figure 21f show statistically significant improvement in the UHDRS TFC finances in 90 mg bid and higher HD1 and HD2 pridopidine treated patients, at 26 weeks.

Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 62 59 54 56 58 Baseline 2 2.2 2.2 2.1 2.2 ∆ to placebo 0.2 0.16 0.27 0.33 p value 0.0853 0.1865 0.0236 0.0061 Figure 21g: Change from baseline in UHDRS TFC ADL Week 26 BL TFC <7. The table below and figure 21g show no significant improvement in the UHDRS TFC ADL in pridopidine treated late stage patients, at 26 weeks. o 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 19 16 25 25 23 Baseline 1.5 1.5 1.4 1.5 1.7 ∆ to placebo -0.19 -0.04 0.18 -0.04 p value 0.3596 0.8518 0.3507 0.8438 Figure 21h: Change from baseline in UHDRS TFC ADL Week 26 BL TFC ≥7. The table below and figure 21h show statistically significant improvement in the UHDRS TFC ADL in 45 mg bid and 90 mg bid and higher pridopidine treated HD1 and HD2 patients, at 26 weeks.

Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 62 59 54 56 58 Baseline 2.6 2.6 2.6 2.6 2.6 ∆ to placebo 0.24 0.16 0.19 0.27 p value 0.0176 0.1132 0.0526 0.0076 Figure 21i: Change from baseline in UHDRS Total Functional Capacity Week 52 BL TFC <7.

The table below and figure 21i show no significant improvement in the UHDRS IS in pridopidine treated late stage patients, at 52weeks.

Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 19 16 25 25 23 ne 4.5 4.1 4.5 4.9 4.7 ∆ to placebo 0.07 -0.5 -0.64 0.1 p value 0.9108 0.3933 0.2828 0.8605 Figure 21j: Change from baseline in UHDRS Total Functional Capacity Week 52 BL TFC ≥7.

The table below and figure 21j show slowing of functional decline as measured by UHDRS TFC in 45 mg bid and 90 mg bid pridopidine treated HD1 and HD2 patients, at 52 weeks.

Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 62 59 54 56 58 Baseline 8.9 9.2 9.4 9.1 9.3 Wk52 ∆ to placebo 1.16 0.36 0.71 0.27 p value 0.0003 0.2704 0.0239 0.4144 Figure 21k: Change from ne in UHDRS TFC Finance ADL Week 52 BL TFC <7. The table below and figure 21k show no significant improvement in the UHDRS TFC finance ADL in late stage pridopidine treated patients, at s.

Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 19 16 25 25 23 Baseline 2 2 1.9 2.2 2.3 ∆ to placebo 0.01 -0.25 -0.29 -0.22 p value 0.9863 0.497 0.4368 0.5626 Figure 21l: Change from baseline in UHDRS TFC e ADL week 52 BL TFC ≥7. The table below and figure 21l show statistically significant improvement in the UHDRS TFC finance ADL in 45 mg bid and 90 mg bid pridopidine treated HD1 and HD2 patients, at 52 weeks.

Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 62 59 54 56 58 ne 4.6 4.7 4.9 4.8 4.7 ∆ to placebo 0.72 0.27 0.53 0.36 p value 0.0004 0.1926 0.0088 0.0841 Figure 21m: Change from baseline in UHDRS TFC Finances Week 52 BL TFC <7. The table below and figure 21m show no significant improvement in the UHDRS TFC finances in pridopidine d late stage patients, at 52weeks.

Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 19 16 25 25 23 Baseline 0.5 0.5 0.5 0.7 0.7 ∆ to placebo 0.29 0.07 0.02 0.04 p value 0.2468 0.7631 0.9318 0.8543 Figure 21n: Change from baseline in UHDRS TFC Finances Week 52 BL TFC ≥7. The table below and figure 21n show statistically significant improvement in the UHDRS IS in 45 mg bid and 90 mg bid pridopidine treated HD1 and HD2 patients, at 52 weeks.

Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 62 59 54 56 58 Baseline 2 2.2 2.2 2.1 2.2 ∆ to placebo 0.35 0.07 0.31 0.12 p value 0.0171 0.6373 0.0332 0.4466 Figure 21o: Change from baseline in UHDRS TFC ADL Week 52 BL TFC <7. The table below and figure 21o show no icant improvement in the UHDRS TFC ADL in pridopidine treated late stage patients, at 52 weeks.

Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 19 16 25 25 23 Baseline 1.5 1.5 1.4 1.5 1.7 ∆ to placebo -0.33 -0.4 -0.39 -0.32 p value 0.178 0.0671 0.073 0.1393 Figure 21p: Change from baseline in UHDRS TFC ADL Week 52 BL TFC ≥7. The table below and figure 21p show statistically significant improvement in the UHDRS TFC ADL in 45 mg bid idine treated HD1 and HD2 patients, at 52 weeks. o 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 62 59 54 56 58 Baseline 2.6 2.6 2.6 2.6 2.6 Wk52 ∆ to placebo 0.35 0.22 0.21 0.23 p value 0.0019 0.0598 0.0545 0.0493 Figure 22a: Change from ne in PBA Irritability, Severity x Frequency Week 52 BL TFC <7. The table below and figure 22a show statistically significant improvement in PBA Irritability in pridopidine treated late stage patients, at 52 weeks.

Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 19 16 25 25 23 ne 1.3 0.9 0.9 1.3 1.6 ∆ to placebo -2.42 -1.78 -1.79 -1.71 p value 0.0165 0.0429 0.0422 0.0542 Figure 22b: Change from baseline in PBA Irritability, Severity x Frequency Week 52 BL TFC ≥7. The table below and figure 22b show statistically significant improvement in the PBA Irritability in 90 mg bid pridopidine treated HD1 and HD2 patients, at 52 weeks.

Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 62 59 54 56 58 Baseline 2.1 1.8 2.1 1.4 1.4 Wk52 ∆ to placebo -0.59 -0.33 -0.95 -0.6 p value 0.1789 0.466 0.0311 0.1927 Figure 23a: Change from baseline in Timed Up and Go Test (sec) Week 26 BL TFC <7. The table below and figure 23a show no significant ement in Timed up and go test in pridopidine treated late stage patients, at 26 weeks.

Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 19 16 25 25 23 Baseline 18.9 13.8 11.9 11.5 16.2 ∆ to placebo 0.5 6.24 1.23 -0.99 p value 0.9181 0.1715 0.7846 0.8295 Figure 23b: Change from baseline in Timed Up and Go Test (sec) Week 26 BL TFC ≥7. The table below and figure 23b show statistically significant improvement in the PBA Irritability in 112.5 mg bid idine treated HD1 and HD2 patients, at 26 weeks.

Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 62 59 54 56 58 Baseline 10 11.7 9.7 9.8 9.8 ∆ to placebo -2.09 -2.41 -2.37 -2.84 p value 0.1397 0.0933 0.0896 0.0478 Figure 24a: Change from baseline in HD-QoL Participant Total Score Week 26 BL TFC <7. The table below and figure 24a show no significant improvement in HD-QoL TS in pridopidine treated late stage patients, at 26 weeks.

Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 19 16 25 25 23 Baseline 94.7 113.1 86.3 83 79.5 ∆ to placebo 4.87 1 2.61 -4.33 p value 0.6958 0.9304 0.817 0.7016 Figure 24b: Change from baseline in HD-QoL ipant Total Score Week 26 BL TFC ≥7. The table below and figure 24b show statistically significant improvement in the PBA Irritability in 67.5 mg bid pridopidine treated HD1 and HD2 patients, at 26 weeks. o 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 62 59 54 56 58 Baseline 61.2 55.1 73.5 68.8 67.3 ∆ to placebo 0.51 10.63 5.17 -0.99 p value 0.9144 0.0284 0.2834 0.8365 Figures 25a-25e show bar graphs of changes in UHDRS TMS Finger Tap scores in 26 and 52 week patient groups.

Figure 25a: Change from ne in UHDRS TMS Finger Taps ALL Week 26. The table below provides P-Values corresponding to Figure 25a. The table below and figure 25a show no significant improvement in the UHDRS TMS finger taps in all pridopidine treated patients, at 26 weeks.

Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 81 75 79 81 81 Baseline 3.8 3.5 4.1 3.7 3.9 ∆ to placebo -0.3 -0.07 -0.07 -0.12 p value 0.1466 0.7306 0.7114 0.5475 Figure 25b: Change from Baseline in UHDRS TMS Finger Taps: Week 26 patients with baseline total functional capacity (BL TFC) ≥ 9 and CAG Repeats > 44. The table below provides the P-Values ponding to Figure 25b. The table below and figure 25b show statistically significant improvement in the UHDRS TMS finger taps in 45 mg bid and 112.5 mg bid pridopidine treated patients having BL TFC greater than or equal to 9 and greater than 44 CAG repeats in their htt gene, at 26 weeks.

Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 13 15 19 22 11 Baseline 2.6 2.7 3.3 3 3.6 ∆ to placebo -0.86 -0.34 -0.52 -1.07 p value 0.0499 0.4255 0.1972 0.0424 Figure 25c: Change from baseline in UHDRS TMS Finger Taps: Week 26 patients with BL TFC ≥ 9, CAG Repeats < 44 and patients who ent three least severe TMS quarters (BL TMS 1st 3 Qs).

The table below provides the P-Values corresponding to Figure 25c. The table below and figure 25c show statistically significant improvement in the UHDRS TMS finger taps in 45 mg bid and 112.5 mg bid pridopidine treated patients having BL TFC r than or equal to 9 and less than 44 CAG repeats in their htt gene, at 26 weeks.

Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 13 15 19 21 10 Baseline 2.6 2.7 3.3 3 3.5 ∆ to placebo -0.87 -0.36 -0.54 -1.05 p value 0.05 0.41 0.1888 0.0537 Figures 25d: Change from baseline in UHDRS TMS Finger Taps: Patients who have completed 52 weeks of treatment: UHDRS TMS Finger Tap score at week 26. The table below provides the s corresponding to Figure 25d. . The table below and figure 25d show statistically significant improvement in the UHDRS TMS finger taps in 45 mg bid pridopidine treated patients who completed 52 weeks, at 26 weeks.

Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 52 43 44 53 44 Baseline 3.8 3.2 4 3.5 3.8 ∆ to placebo -0.59 -0.13 -0.01 -0.21 p value 0.0182 0.5881 0.9554 0.3833 s 25e: Change from baseline in UHDRS TMS Finger Taps: Patients who have completed 52 weeks of ent: UHDRS TMS Finger Tap score at week 52. The table below provides the P- Values corresponding to Figure 25e. The table below and figure 25e show no significant ement in the UHDRS TMS finger taps in ALL pridopidine treated patients, at 52 weeks.

Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 52 43 44 53 44 Baseline 3.8 3.2 4 3.5 3.8 ∆ to placebo -0.31 0.13 0.08 0.1 p value 0.2091 0.6027 0.7179 0.6835 Figure 26a: Change from baseline in UHDRS TMS Finger Tapping + Pronate-Supinate Hands: ts who have ted 52 weeks of treatment – score at week 26. The table below provides the P-Values corresponding to Figure 26a. The table below and figure 26a show statistically significant improvement in the UHDRS TMS finger taps and Pronate-Supinate Hands in 45 mg bid pridopidine treated patients who completed 52 weeks, at 26 weeks.

Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 52 43 44 53 44 Baseline 7.1 6.1 7 6.5 7 ∆ to placebo -0.79 0.02 0.02 -0.23 p value 0.0294 0.9443 0.9412 0.5268 Figure 26b: Change from baseline in UHDRS TMS Finger g + Pronate-Supinate Hands: ts who have completed 52 weeks of treatment – score at week 52. The table below provides the P-Values corresponding to Figure 26b. The table below and figure 26b show no significant improvement in the UHDRS TMS finger taps and Pronate-Supinate Hands in pridopidine treated patients at 26 weeks.

Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 52 43 44 53 44 Baseline 7.1 6.1 7 6.5 7 ∆ to placebo -0.37 0.68 0.48 0.28 p value 0.3801 0.1066 0.2337 0.4978 Figure 27a: Change from ne in UHDRS TMS Gait and Balance: Gait and balance scores at week 26 for patients with BL TFC ≥ 7. The table below provides the P-Values corresponding to Figure 27a. The table below and figure 27a show statistically significant improvement in the UHDRS TMS gait and balances in 90 mg bid pridopidine treated HD1 and HD2 patients at 26 weeks.

Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 62 59 54 56 58 Baseline 3.2 3.7 3.4 3.5 3.1 ∆ to placebo -0.48 -0.37 -0.62 -0.49 p value 0.0563 0.1442 0.013 0.0518 Figure 27b: Change from ne in UHDRS TMS Gait and Balance: Gait and balance scores at week 52 for patients with BL TFC ≥ 7. The table below provides the P-Values corresponding to Figure 27b. The table below and figure 27b show no significant improvement in the UHDRS TMS gait and balances in pridopidine treated HD1 and HD2 patients at 52 weeks.

Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 62 59 54 56 58 Baseline 3.2 3.7 3.4 3.5 3.1 ∆ to placebo -0.41 -0.43 -0.28 -0.09 p value 0.1811 0.1691 0.365 0.7719 Figures 28a-28d provide bar graphs g change from baseline in UHDRS TMS Dystonia scores in 26 and 52 week t groups.

Figure 28a: Change from ne in UHDRS TMS Dystonia ALL: UHDRS TMS Dystonia scores at week 26 in all ts. The table below provides the P-Values corresponding to Figure 28a. No significant improvement is observed.

Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 81 75 79 81 81 Baseline 4.1 3.6 4.1 4.9 4.5 ∆ to placebo -0.06 -0.34 -0.33 -0.29 p value 0.8711 0.3778 0.3845 0.4507 Figure 28b: Change from baseline in UHDRS TMS Dystonia: UHDRS TMS Dystonia scores for patients with BL TFC ≥ 9 AND CAG Repeats < 44 at week 26. The table below provides the P-Values corresponding to Figure 28b. Patients with baseline TFC greater than or equal to 9, show statistically significant improvement in the UHDRS TMS Dystonia score at 45 mg bid, 67.5 bid and 90 mg bid idine for 26 weeks.

Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 13 15 19 22 11 Baseline 3.8 1.7 2.8 3.4 1.9 ∆ to placebo -1.54 -1.58 -1.72 -1.4 p value 0.0313 0.0191 0.0078 0.0847 Figure 28c: Change from baseline in UHDRS TMS Dystonia: UHDRS TMS ia scores for patients with CAG Repeats < 44 AND BL TMS 1st 3 Qs at week 26. The table below provides the PValues corresponding to Figure 28c. ts with baseline TMS who represent three least severe TMS quarters and less than 44 CAG repeats in their htt gene, show statistically significant improvement in the UHDRS TMS Dystonia score at 45 mg bid, 67.5 bid and 90 mg bid pridopidine for 26 weeks.

Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 29 29 32 37 22 Baseline 3 2.6 2.6 2.9 2.6 ∆ to placebo -1.04 -1.15 -1 -0.62 p value 0.0437 0.0235 0.0399 0.2655 Figure 28d: Change from baseline in UHDRS TMS Dystonia: UHDRS TMS Dystonia scores for ts with BL TFC ≥ 9 and CAG Repeats < 44 and BL TMS 1st 3 Qs at week 26. The table below provides the P-Values corresponding to Figure 28d. Patients with baseline TFC greater than or equal to 9, baseline TMS representing three least severe TMS quarters and less than 44 CAG repeats in their htt gene, show statistically significant improvement in the UHDRS TMS Dystonia score at 45 mg bid 67.5 mg bid and 90 mg bid pridopidine for 26 weeks.

Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 13 15 19 21 10 Baseline 3.8 1.7 2.8 3.1 2.1 ∆ to o -1.53 -1.6 -1.64 -1.29 p value 0.0349 0.02 0.0132 0.1276 Figures 29a, 29b and 29c are bar graphs showing changes from baseline in Gait and Balance scores at week 12 (29a); week 20 (29b); and week 26 (29c). Y-axes are changes in UHDRS Gait and Balance score.

Figure 30 is a graph showing changes from baseline in UHDRS TFC score over 26 weeks for treatment with pridopidine and placebo. The data for 112.5 mg idine bid is shown by the top line in this graph and the data for the placebo is shown by the bottom line in this graph. Difference in p-value of 112.5 mg idine bid from placebo was 0.1498 at week 4, 0.6065 at week 12, 0.3238 at week 20, and 0.0676 at week 26. Increase in Change in UHDRS TFC indicates delay/reduction in functional decline.

Figures 31a-31n provide bar graphs or line graphs showing changes from baseline of UHDRS TFC scores in 26 and 52 week patient groups.

Figures 31a and 31b show change from baseline in UHDRS TFC score over time. Y axes represents change in TFC score, X axes represents pridopidine treatment time, in weeks. Figure 31a shows the trend in full analysis set after 52 weeks. Figure 31b shows trends in patients having BL TFC ≥7 (n=54- Figure 31c: Change from baseline in UHDRS Total Functional ty for ts with BL CAG Repeats < 44 at week 26. The table below provides the P-Values corresponding to Figure 31c.

Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 37 37 38 42 29 Baseline 7.4 7.9 8.5 8.3 7.9 ∆ to placebo 0.6 0.43 0.79 0.38 p value 0.056 0.1707 0.0102 0.2643 Figure 31d: Change from ne in UHDRS Total Functional Capacity for ts with BL TFC ≥ 9 or CAG Repeats < 44 at week 26. The table below provides the P-Values corresponding to Figure Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 56 56 53 52 49 Baseline 8.5 8.8 8.9 8.7 9.1 ∆ to placebo 0.56 0.18 0.67 0.38 p value 0.0321 0.5069 0.0117 0.1665 Figure 31e: Change from baseline in UHDRS Total Functional Capacity for patients with BL CAG Repeats < 44 AND BL TMS 1st 3 Qs at week 26. The table below provides the P-Values ponding to Figure 31e.

Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 29 29 32 37 22 Baseline 8 8.7 9 8.6 8.6 ∆ to placebo 0.73 0.47 0.71 0.48 p value 0.0469 0.1952 0.0405 0.2324 Figure 31f: Change from baseline in UHDRS Total Functional Capacity for patients with BL CAG Repeats < 44 OR BL TMS 1st 3 Qs at week 26 (baseline TMS in the first 3 quartiles). The table below provides the P-Values corresponding to Figure 31f.

Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 69 65 66 66 68 Baseline 8.1 8.4 8.3 8.3 8.5 ∆ to placebo 0.36 0.18 0.52 0.51 p value 0.1493 0.4727 0.0349 0.0379 Figure 31g: Change from baseline in UHDRS Total Functional ty for patients with Week 26 median BL TFC OR CAG Repeats < 44 or BL TMS 1st 3 Qs at week 26. The table below provides the P-Values corresponding to Figure 31g.

Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 71 67 66 66 69 ne 8.2 8.5 8.3 8.3 8.5 ∆ to placebo 0.36 0.21 0.55 0.53 p value 0.1423 0.3863 0.0244 0.0289 Figure 31h: Change from baseline in UHDRS Total Functional Capacity for patients with BL TFC ≥ 9 or BL TMS 1st 3 Qs at week 26. The table below provides the P-Values corresponding to Figure Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 30 32 34 31 29 Baseline 10.3 10.5 10.4 10.3 10.9 ∆ to placebo 0.69 0.01 0.54 0.6 p value 0.0601 0.9741 0.1371 0.1136 Figure 32: A graph showing changes from baseline in es and ADL TFC scores over 26 weeks for ent with pridopidine and placebo. The data for 112.5 mg pridopidine bid is shown by the top line in this graph and the data for the placebo is shown by the bottom line in this graph. Difference in p-value of 112.5 mg pridopidine bid from placebo was 0.4382 at week 4, 0.6636 at week 12, 0.4437 at week 20, and 0.0125 at week 26. An increase in Change in TFC Finance and ADL indicates a lessening in onal decline.

Figures 33a, 33b, and 33c: Change from baseline in TFC score in pridopidine treated HD patients.

Doses at week 12 (Fig. 33a), week 20 (Fig. 33b) and week 26 (Fig. 33c). Score is adjusted means + SE of change in TFC for full analysis set.

Figures 34a, 34b, and 34c: Change from baseline in TFC ADL & Finances score in pridopidine treated HD patients. Doses at week 12 (34a), week 20 (34b) and week 26 (34c). Score is adjusted means + SE of change in TFC e and ADL for full is set.

Figures s are bar graphs showing changes from baseline of UHDRS TFC Finances and UHDRS TFC es and ADL scores in 26 and 52 week patient groups according to quartiles.

Figure 35a: Change from baseline in UHDRS TFC Finances score for patients with TMS 1st Q (first least severe TMS quarter) at week 26. The table below provides the P-Values corresponding to Figure 35a. Significant improvement in TFC finances in 45 mg bid pridopidine administered first least severe TMS quarter patients for 26 weeks.

Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 21 24 20 25 15 ne 2.2 2.1 2.3 2.2 2.6 ∆ to placebo 0.38 0.27 0.26 0.63 p value 0.0347 0.1556 0.1336 0.0038 Figure 35b: Change from baseline in UHDRS TFC Finances score for patients with TMS 1st Q at week 52. The table below provides the P-Values corresponding to Figure 35b. Trend towards improvement in TFC finances was observed in 45 mg bid pridopidine administered first least severe TMS quarter patients for 52 weeks.

Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 21 24 20 25 15 Baseline 2.2 2.1 2.3 2.2 2.6 ∆ to placebo 0.43 0.25 0.21 0.32 p value 0.0673 0.3084 0.3653 0.2369 Figure 35c: Change from baseline in UHDRS TFC Finances score for patients with TMS 1st 2Qs (first two least severe TMS quarters) at week 26. The table below provides the P-Values ponding to Figure 35c. Trend towards improvement in TFC finances was observed in 45 mg bid pridopidine administered first two least severe TMS quarter patients for 26 weeks.

Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 42 44 39 40 43 Baseline 2 2.1 2.2 2.1 2.1 ∆ to placebo 0.33 0.04 0.14 -0.06 p value 0.0566 0.8406 0.4275 0.7529 Figure 35d: Change from ne in UHDRS TFC Finances score for patients with TMS 1st 2Qs at week 52. The table below provides the P-Values corresponding to Figure 35d. Significant improvement in TFC finances was observed in 45 mg bid idine administered first two least severe TMS quarters patients for 52 weeks. o 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 42 44 39 40 43 Baseline 2 2.1 2.2 2.1 2.1 ∆ to placebo 0.29 0.15 0.23 0.2 p value 0.0299 0.2941 0.0994 0.1432 Figure 35e: Change from baseline in UHDRS TFC Finances score for patients with TMS 1st 3Qs at week 26. The table below provides the P-Values corresponding to Figure 35e. Trend towards improvement in TFC finances in 45 mg bid pridopidine administered first three least severe TMS quarter patients for 26 weeks.

Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 58 59 62 58 62 Baseline 1.8 2 1.9 1.9 2 ∆ to placebo 0.12 0.03 0.22 0.26 p value 0.315 0.8115 0.0665 0.0323 Figure 35f: Change from baseline in UHDRS TFC Finances score for patients with TMS 1st 3Qs at week 52. The table below es the P-Values corresponding to Figure 35f. Significant improvement in TFC finances was observed in 45 mg bid pridopidine administered first three least severe TMS quarter patients for 52 weeks.

Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 58 59 62 58 62 ne 1.8 2 1.9 1.9 2 ∆ to placebo 0.39 0.01 0.17 0.05 p value 0.0072 0.97 0.2295 0.7396 Figure 35g: Change from baseline in UHDRS TFC Finance and ADL score for patients with BL TFC ≥ 9 at week 26. The table below provides the P-Values corresponding to Figure 35g. Significant ement in TFC finance and ADL was observed in 45 mg bid and 90 mg bid pridopidine administered patients having with baseline TFC greater than or equal to 9, for 26 weeks.

Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 32 34 34 32 31 Baseline 5.2 5.1 5.4 5.4 5.4 ∆ to placebo 0.53 0.23 0.51 0.57 p value 0.0143 0.2874 0.0197 0.0109 Figure 35h: Change from baseline in UHDRS TFC Finance and ADL score for patients with BL CAG Repeat > 44 at week 26. The table below provides the P -Values corresponding to Figure 35h.

Significant ement in TFC finance and ADL was observed in 45 mg bid and 90 mg bid pridopidine administered patients having more than 44 CAG repeats in their htt gene, for 26 weeks.

Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 37 37 38 42 29 Baseline 3.7 4.1 4.2 4.2 4.1 ∆ to placebo 0.55 0.21 0.67 0.47 p value 0.017 0.3497 0.0026 0.0597 Figure 35i: Change from baseline in UHDRS TFC Finance and ADL score for patients with BL TFC ≥ 9 and CAG Repeat >44 at week 26. The table below provides the P-Values corresponding to Figure 35i. Significant improvement in TFC finance and ADL was observed in 45 mg bid and 90 mg bid pridopidine administered patients having baseline TFC greater than or equal to 9 and more than 44 CAG repeats in their htt gene, for 26 weeks.

Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 13 15 19 22 11 Baseline 5.1 5.2 5.5 5.4 5.5 ∆ to placebo 0.74 0.57 0.83 1.02 p value 0.0296 0.083 0.0089 0.0094 Figure 35j: Change from baseline in UHDRS TFC Finance and ADL score for patients with BL TFC ≥ 9 or CAG Repeat > 44 at week 26. The table below provides the P-Values corresponding to Figure 35j Significant improvement in TFC finance and ADL was observed in 45 mg bid and 90 mg bid pridopidine administered patients having baseline TFC greater than or equal to 9 or more than 44 CAG s in their htt gene, for 26 weeks.

Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 56 56 53 52 49 Baseline 4.2 4.5 4.5 4.5 4.7 ∆ to placebo 0.5 0.08 0.52 0.4 p value 0.0055 0.6381 0.0039 0.0317 Figure 35k: Change from ne in UHDRS TFC Finance and ADL score for patients with CAG Repeats < 44 and BL TMS 1st 3 Qs at week 26. The table below provides the es corresponding to Figure 35k. Significant improvement in TFC finance and ADL was observed in 45 mg bid and 90 mg bid idine administered patients having ne TMS first 3 quarters and less than 44 CAG repeats in their htt gene, for 26 weeks.

Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 29 29 32 37 22 ne 4.1 4.6 4.5 4.4 4.6 ∆ to placebo 0.59 0.18 0.6 0.57 p value 0.0236 0.4782 0.0145 0.0478 Figure 35l: Change from baseline in UHDRS TFC Finance and ADL score for patients with BL TFC ≥ 9 and CAG Repeats < 44 and BL TMS 1st 3 Qs at week 26. The table below provides the P-Values corresponding to Figure 35l. Significant improvement in TFC finance and ADL was observed in 45 mg bid and 90 mg bid pridopidine stered patients having baseline TFC r than or equal to 9 and less than 44 CAG repeats in their htt gene, for 26 weeks.

Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 13 15 19 21 10 Baseline 5.1 5.2 5.5 5.3 5.5 ∆ to placebo 0.74 0.57 0.81 1.08 p value 0.0315 0.0848 0.0118 0.009 Figure 35m: Change from baseline in UHDRS TFC Finance and ADL score for patients with BL TFC ≥ 9 and BL TMS 1st 3 Qs at week 26. The table below provides the es corresponding to Figure 35m. Significant improvement in TFC finance and ADL was observed in 45 mg bid and 90 mg bid idine administered patients having baseline TFC greater than or equal to 9 or less than 44 CAG repeats in their htt gene or baseline TMS first three quarters, for 26 weeks.

Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 30 32 34 31 29 Baseline 5.1 5.1 5.4 5.4 5.5 ∆ to placebo 0.53 0.18 0.45 0.54 p value 0.018 0.4039 0.0455 0.0193 Figure 35n: Change from baseline in UHDRS TFC Finance and ADL score for patients with TMS 1st Q at week 26. The table below provides the P-Values corresponding to Figure 35n. Significant improvement in TFC finance and ADL was observed in 45 mg bid and 90 mg bid pridopidine stered patients with TMS first three quarters, for 26 weeks.

Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 21 24 20 25 15 Baseline 4.9 4.8 5 4.8 5.3 ∆ to placebo 0.63 0.5 0.63 1.1 p value 0.038 0.1136 0.0342 0.0024 Figure 35o: Change from baseline in UHDRS TFC e and ADL score for patients with TMS 1st Q at week 52. The table below provides the P-Values corresponding to Figure 35o. Significant ement in TFC finance and ADL was observed in 45 mg bid pridopidine administered patients with TMS first quarter, for 52 weeks.

Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 21 24 20 25 15 Baseline 4.9 4.8 5 4.8 5.3 ∆ to placebo 0.71 0.61 0.57 0.74 p value 0.0319 0.0744 0.0762 0.0534 Figure 35p: Change from baseline in UHDRS TFC Finance and ADL score for patients with TMS 1st 2Qs at week 26. The table below provides the P-Values corresponding to Figure 35p. Significant improvement in TFC finance and ADL was observed in 45 mg bid pridopidine administered patients with TMS first two quarters, for 26 weeks.

Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 42 44 39 40 43 Baseline 4.5 4.7 4.8 4.6 4.7 ∆ to placebo 0.48 0.1 0.26 0.19 p value 0.045 0.6867 0.3021 0.4543 Figure 35q: Change from baseline in UHDRS TFC Finance and ADL score for patients with TMS 1st 2Qs at week 52. The table below provides the P-Values corresponding to Figure 35q. icant improvement in TFC finance and ADL was observed in 45 mg bid and 90 mg bid pridopidine administered ts with TMS first two quarters, for 52 weeks.

Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 42 44 39 40 43 Baseline 4.5 4.7 4.8 4.6 4.7 ∆ to placebo 0.47 0.25 0.47 0.44 p value 0.0294 0.255 0.0326 0.0433 Figure 35r: Change from baseline in UHDRS TFC Finance and ADL score for patients with TMS 1st 3Qs at week 26. The table below provides the P-Values corresponding to Figure 35r. No significant improvement in TFC e and ADL was observed in pridopidine administered patients with TMS first three quarters, for 26 weeks.

Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 58 59 62 58 62 Baseline 4.3 4.5 4.3 4.4 4.5 ∆ to placebo 0.18 0.04 0.35 0.41 p value 0.3393 0.8205 0.0555 0.0253 Figure 35s: Change from baseline in UHDRS TFC Finance and ADL score for patients with TMS 1st 3Qs at week 52. The table below provides the P-Values corresponding to Figure 35s. Significant improvement in TFC finance and ADL was observed in 45 mg bid idine administered patients with TMS first three quarters, for 52 weeks. o 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 58 59 62 58 62 Baseline 4.3 4.5 4.3 4.4 4.5 ∆ to placebo 0.52 -0.03 0.21 0.09 p value 0.0122 0.8661 0.3033 0.6679 Figures 36a and 36b: General information regarding Finger tapping (Q -motor tap ements).

Figure 36a shows a drawing of subject’s arm with tapper. Figure 36b shows normal and aberrant g measurements.

Figures 37a and 37b: Q -motor tap measurements: A well-validated objective measure. (Bechtel 2010).

Figure 38: Q -Motor Tap-Speed-Frequency. 90mg idine administered bid demonstrated consistent improvement from ne. The data for 90 mg bid is shown by the top line in this graph and the data for the placebo is shown by the bottom line in this graph. Difference in p-value of 90 mg bid from placebo was 0.0259 at week 4, 0.0365 at week 12, and 0.0056 at week 26. Increase in tap speed indicates improvement. The unit of measurement of the Y-axis is Frequency (Hz).

Figures 39a and 39b: Q-Motor Tap Speed Inter Onset Interval (IOI). 90mg pridopidine administered bid demonstrated tent and significant improvement from baseline for 90mg bid. The data for 90 mg pridopidine bid is shown by the bottom line in this graph and the data for the placebo is shown by the top line in this graph. Difference in p-value of 90 mg pridopidine bid from placebo was 0.0342 at week 4, 0.0368 at week 12, and 0.0162 at week 26. se in inter tap interval indicates improvement. The unit of measurement of the Y-axis in Figure 39a is Frequency (Hz). Figure 39b shows change from baseline in Tap-Speed-Inter-Onset-interval-MN-Hand-L (sec) over time ) for full analysis set.

Figure 39c: Improvement in objective pharmacodynamic measures of motor control: change from baseline in Q-Motor: Tap-Speed-Inter-Onset-interval-MN-Hand (sec), Week 52 FAS. The table below provides data and the P-Values corresponding to Figure 39c. A trend towards improvement was noted in 45 mg bid treated patients.

Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 81 75 79 81 81 Baseline 0.4065 0.4154 0.4608 0.4029 0.4366 ∆ to placebo -0.0402 0.0152 -0.0064 -0.017 p value 0.1956 0.6063 0.8258 0.5689 Figure 39d: Improvement in objective pharmacodynamic measures of motor l: change from baseline in Q-Motor: Tap-Speed-Inter-Onset-interval-MN-Hand (sec), Week 52 in pridopidine treated HD1 and HD2 patients. The table below provides the data and P-Values ponding to Figure 39d.

A trend towards improvement was noted in all treatment arms.

Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 62 59 54 56 58 Baseline 0.3725 0.3605 0.3983 0.3789 0.4056 ∆ to placebo -0.0351 4 -0.0291 -0.022 p value 0.1347 0.0449 0.2039 0.3509 Figure 39e: Improvement in ive pharmacodynamic measures of motor control, change from baseline in Q-Motor: Pro-Sup-Frequency-MN-Hand (Hz), Week 52 FAS. The table below provides the data and P-Values corresponding to Figure 39e. A trend towards improvement was noted in 45 mg bid treated patients.

Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 81 75 79 81 81 Baseline 1.6686 1.7789 1.7255 1.7505 1.7251 Wk52 ∆ to placebo 0.0599 -0.0124 -0.0087 0.0127 p value 0.3122 0.8278 0.8763 0.8261 Figure 39f: Improvement in objective codynamic es of motor control, change from baseline in Q-Motor: Pro-Sup-Frequency-MN-Hand (Hz), Week 52 Week 52 in pridopidine treated HD1 and HD2 patients. The table below provides the data and P-Values corresponding to Figure 39f.

A trend towards improvement was noted in 45 mg bid treated patients.

Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 62 59 54 56 58 Baseline 1.77 1.8513 1.8928 1.8658 1.841 Wk52 ∆ to placebo 0.1195 0.0548 0.0575 0.08 p value 0.0692 0.3996 0.3709 0.229 Figure 40a: Change from baseline in Cognitive Assessment Battery Hopkins Verbal Learning Test, revised (CAB HVLT-R) score for patients at week 26. The table below provides the P-Values corresponding to Figure 40a. No significant improvement in CAB HVLT-R score was observed in pridopidine administered patients, for 26 weeks.

Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 81 75 79 81 81 Baseline 19.3 19.5 20.3 19.4 19.1 ∆ to o -0.53 0.15 -0.73 -0.47 p value 0.5837 0.8758 0.4384 0.6217 Figure 40b: Change from ne in ive Assessment Battery Hopkins Verbal Learning Test, revised (CAB HVLT-R) score for ts at week 52. The table below provides the P-Values corresponding to Figure 40b. A trend towards improvement in CAB HVLT-R score was observed in 45 mg bid pridopidine administered patients, for 52 weeks.

Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 81 75 79 81 81 Baseline 19.3 19.5 20.3 19.4 19.1 ∆ to placebo -2.21 -2.74 -1.07 -2.19 p value 0.0517 0.0148 0.3265 0.0562 Figure 41a: Change from baseline in Cognitive ment Battery CAB Trail Making Test score for patients at week 26. The table below provides the P-Values corresponding to Figure 41a. No significant improvement in CAB Trail making test score was ed in pridopidine administered patients, for 26 weeks.

Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 81 75 79 81 81 Baseline -184.7 -181.6 -182.2 -185 -178.9 ∆ to placebo -0.95 -3.03 -6.64 2.97 p value 0.8773 0.6211 0.2713 0.6283 Figure 41b: Change from baseline in Cognitive Assessment Battery CAB Trail Making Test score for patients at week 52. The table below provides the P-Values corresponding to Figure 41b. A trend towards improvement in CAB Trail making test score was observed in pridopidine administered patients, for 52 weeks.

Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 81 75 79 81 81 Baseline -184.7 -181.6 -182.2 -185 -178.9 ∆ to placebo -13.56 -7.54 -12.48 2.01 p value 0.0773 0.3266 0.0913 0.7951 Figure 41c: Change from baseline in Cognitive Assessment Battery CAB Paced Tapping at 3Hz at 26 weeks. The table below provides data and the P-Values corresponding to Figure 41c.

Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 81 75 79 81 81 Baseline 5.935 6.035 5.027 4.943 5.572 ∆ to placebo 0.4736 -0.041 0.1975 0.9515 p value 0.4081 0.9441 0.7276 0.0937 Figure 41d: Change from baseline in Cognitive Assessment Battery CAB Paced Tapping at 3Hz at 52 weeks. The table below provides data and the P-Values corresponding to Figure 41d.

Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 81 75 79 81 81 Baseline 5.935 6.035 5.027 4.943 5.572 ∆ to placebo 1.3234 0.3701 0.2659 0.1523 p value 0.0402 0.5681 0.6681 0.8152 Figure 42: Annual rates of e (y axis) in TFC are higher in earlier stages of e (Marder 2000).

Figure 43a: Mean change from baseline TFC in placebo arms of (1) Open -label Extension Study of Pridopidine ) in the matic Treatment of Huntington Disease (OPEN-HART) (n=50), (2) Co-Enzyme Q10 And mide: Evaluation in HD (CARE-HD) (n=80) (Kieburtz 2001) and (3) Coenzyme Q10 in Huntington's Disease (HD) ) (n=213): TFC Score Change From Baseline (non-matched cohorts). The circle over the 12 months points reflects ~1-point difference that was ed in the rate of functional decline in Open-HART subjects treated with idine.

Figure 43b: Change from baseline in TFC score plotted over time in Week 52 in pridopidine treated HD1 and HD2 treated subjects (n=54-62) in HD trial. The dark line with diamond represents placebo; line with open circle represents 45 mg bid, line with triangle represents 67.5 mg bid, line with grey diamond represents 90 mg bid, line with square represents 112.5 mg bid. Y axis represents change from baseline in TFC score from baseline, x axis represents treatment time in weeks. s 44a-44c are graphs which show le ambulation-related endpoints demonstrating trends favoring pridopidine in early HD (stage 1-2 patients). Data for TMS showed a strong placebo . A trend towards improvement in TMS was observed at 52 weeks.

Figure 44a: UHDRS TMS Gait: Early HD at 52 weeks. The table below provides data and the P- Values corresponding to Figure 44a.

Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 62 59 54 56 58 Baseline 0.9 1.1 1 1.1 1 Wk52 ∆ to placebo -0.21 -0.17 -0.17 -0.06 p value 0.0855 0.168 0.1521 0.628 Figure 44b: Timed Up and Go Test (sec): idine treated HD1 and HD2 patients.at 52 weeks. The table below provides data and the P-Values ponding to Figure 44b.

Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 62 59 54 56 58 Baseline 10 11.7 9.7 9.8 9.8 Wk52 ∆ to placebo -1.61 -1.64 -1.46 -0.96 p value 0.1348 0.1369 0.171 0.3827 Figure 44c: Walk -12 improved in pridopidine treated HD1 patients at 52 weeks. The table below provides data and the P-Values corresponding to Figure 44c.

Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 12 17 17 11 18 Baseline 21.2 6.3 12.3 17.7 13 ∆ to placebo -5.86 -8.57 -13.6 -4.13 p value 0.3018 0.1032 0.0193 0.4534 Figures 44d and 44e: Week 26 and week 52 HD, respectively, Pridopidine treated HD1 patients for Involuntary movements: Total Maximal Chorea (TMC).

The table below provides the data and P-Values ponding to Figure 44d.

Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 12 17 17 11 18 Baseline 12 17 17 11 18 ∆ to placebo -1.4 -2.07 -2.52 -1.08 p value 0.1805 0.0438 0.0271 0.2932 The table below provides the data and P-Values corresponding to Figure 44e.

Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 12 17 17 11 18 Baseline 12 17 17 11 18 ∆ to placebo -0.93 -0.13 -2.43 0.88 p value 0.5315 0.931 0.1313 0.5622 Figures 45a-45b: Figure 45: Change from baseline in TMS plotted over time in TMS full analysis set plotted over time in PRIDE-HD. Data replicates previous data in changes from baseline in TMS as change from baseline values were similar to HART and HD. A se in TMS change from baseline indicates ement. Y axis represents change from baseline in TMS from baseline, x axis represents treatment time in weeks. Figure 45b: Change from baseline in TMS d over time in HD1 patients. Line with dark diamond represents placebo; line with open circle represents 45 mg bid, line with triangle represents 67.5 mg bid, line with grey diamond ents 90 mg bid, line with square represents 112.5 mg bid. 45 mg bid shows improvement in TMS score after 52 weeks. Y axis represents change from baseline in TMS from baseline, x axis represents treatment time in weeks.

Figures 46a-46v and 47a-47y, show ambulation related Modified Physical Performance Test (mPPT) data. The table provided below each graph provides data and P-values ponding to the graph.

Figure 46a: Change from baseline in mPPT Total Score, Full Analysis Set from week 4 of treatment through week 52. The mPPT quantifies the t's performance in physical and functional tasks using a standardized 9-item test. Line with dark diamond represents placebo; line with open circle represents 45 mg bid, line with triangle represents 67.5 mg bid, line with grey diamond represents 90 mg bid, line with square represents 112.5 mg bid. Administration of 45 mg bid shows improvement on mPPT score after 52 weeks. Y axis represents change from baseline in mPPT from baseline, x axis represents treatment time in weeks.

Figure 46b: mPPT Total Score – Change from Baseline, Full Analysis Set, week 26. The table below provides the data and P-Values corresponding to Figure 46b.

Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 81 75 79 81 81 Baseline 25.5 24.9 26.1 25.7 25.7 ∆ to o 0.04 -0.07 -0.01 0.29 p value 0.9462 0.8968 0.9853 0.6063 Figure 46c: mPPT Total Score – Change from Baseline, Full Analysis Set, week 52. The table below provides the data and P-Values ponding to Figure 46c.

Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 81 75 79 81 81 Baseline 25.5 24.9 26.1 25.7 25.7 ∆ to placebo 0.46 0.17 -0.33 -0.47 p value 0.5541 0.8284 0.661 0.5482 Figure 46d: mPPT Tota l Score – Change from Baseline in pridopidine treated patients with baseline TFC <7 week 26. The table below provides the data and P-Values corresponding to Figure 46d.

Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 19 16 25 25 23 Baseline 21.5 21.4 23.3 23.1 21.3 ∆ to placebo 0.9 -0.85 -0.73 -0.33 p value 0.5266 0.5247 0.5786 0.802 Figure 46e: mPPT Tot al Score – Change from Baseline in pridopidine treated HD1 and HD2 ts week 26. The table below provides the data and P-Values corresponding to Figure 46e. o 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 62 59 54 56 58 Baseline 26.6 25.9 27.4 26.9 27.5 ∆ to placebo -0.24 0.59 0.46 0.79 p value 0.6903 0.3296 0.4429 0.1889 Figure 46f: mPPT Tot al Score – Change from Baseline in idine treated patients with baseline TFC <7 week 52. The table below provides the data and P-Values corresponding to Figure 46f.

Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 19 16 25 25 23 Baseline 21.5 21.4 23.3 23.1 21.3 ∆ to placebo 1.57 -1.37 -2.73 -0.49 p value 0.4267 0.4515 0.1288 0.7822 Figure 46g: mPPT Tota l Score – Change from Baseline, in pridopidine d HD1 and HD2 patients week 52. The table below es the data and P-Values ponding to Figure 46g.

Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 62 59 54 56 58 Baseline 26.6 25.9 27.4 26.9 27.5 ∆ to placebo 0.15 1.04 0.88 -0.26 p value 0.8564 0.2087 0.2728 0.7532 Figure 46h: Change from baseline in mPPT total score in pridopidine treated HD1 patients at 26 weeks. The table below provides the data and P-Values corresponding to Figure 46h.

Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 12 17 17 11 18 Baseline 28.1 30.1 28.7 27.8 30.2 ∆ to placebo -1.31 -0.2 0.81 0.03 p value 0.2537 0.8574 0.5048 0.9789 Figure 46i: Change from baseline in mPPT total score in pridopidine treated HD2 patients at 26 weeks. The table below provides the data and P-Values corresponding to Figure 46i.

Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 50 42 37 45 40 Baseline 26.3 24.1 26.8 26.7 26.3 ∆ to placebo -0.07 0.68 0.35 0.85 p value 0.9231 0.3603 0.6191 0.242 Figure 46j: Change from baseline in mPPT total score in pridopidine treated HD patients BL stage 3- (TFC 0-6) at 26 weeks. The table below provides the data and P-Values corresponding to Figure 46j.

Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 19 16 25 25 23 Baseline 21.5 21.4 23.3 23.1 21.3 ∆ to placebo 0.9 -0.85 -0.73 -0.33 p value 0.5266 0.5247 0.5786 0.802 Figure 46k: Change from baseline in mPPT total score in pridopidine treated HD1 patients at 52 weeks. The table below provides the data and P-Values corresponding to Figure 46k.

Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 12 17 17 11 18 Baseline 28.1 30.1 28.7 27.8 30.2 ∆ to placebo 0.76 0.45 1.11 0.08 p value 0.5292 0.7013 0.388 0.9456 Figure 46l: Change from baseline in mPPT total score in pridopidine treated HD2 patients at 52 weeks. The table below provides the data and es corresponding to Figure 46l.

Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 50 42 37 45 40 Baseline 26.3 24.1 26.8 26.7 26.3 ∆ to placebo -0.39 1 0.71 -0.65 p value 0.7028 0.3539 0.4672 0.5427 Figure 46m: Change from baseline in mPPT total score in pridopidine d HD patients BL stage 3-5 at 52 weeks. The table below provides the data and P-Values corresponding to Figure 46m. o 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 19 16 25 25 23 Baseline 21.5 21.4 23.3 23.1 21.3 ∆ to placebo 1.57 -1.37 -2.73 -0.49 p value 0.4267 0.4515 0.1288 0.7822 Figure 46n: Graph showing change from baseline in mPPT standing static balance scores, full is set from week 4 of ent through week 52. Line with dark diamond represents placebo; line with open circle represents 45 mg bid, line with triangle represents 67.5 mg bid, line with grey diamond represents 90 mg bid, line with square represents 112.5 mg bid. s 46o: mPPT standing static balance scores, full analysis set at 26 weeks. The table below provides the data and P-Values corresponding to Figure 46o.

Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 81 75 79 81 81 Baseline 3.1 2.9 2.9 3.1 3 ∆ to placebo 0.07 -0.2 -0.1 0.05 p value 0.6768 0.2154 0.5123 0.7294 Figure 46p: mPPT standing st atic balance scores, full analysis set at 52 weeks. The table below provides the data and P-Values corresponding to Figure 46p.

Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 81 75 79 81 81 Baseline 3.1 2.9 2.9 3.1 3 ∆ to placebo -0.15 -0.19 -0.14 -0.12 p value 0.4019 0.3018 0.435 0.5024 Figure 46q: graph showing change from ne in mPPT Chair Rise scores, full analysis set from week 4 of ent through week 52. Line with dark diamond represents placebo; line with open circle ents 45 mg bid, line with triangle represents 67.5 mg bid, line with grey diamond represents 90 mg bid, line with square represents 112.5 mg bid.

Figure 46r: mPPT Chair Rise scores, full analysis set at 26 weeks. The table below provides the data and P-Values corresponding to Figure 46r.

Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 81 75 79 81 81 Baseline 2.5 2.6 2.8 2.8 2.6 ∆ to placebo 0.05 0.01 0.01 0.12 p value 0.7238 0.9436 0.9222 0.3883 Figure 46s: mPPT Chair Rise scores, full ana lysis set at 52 weeks. The table below provides the data and es corresponding to Figure 46s.

Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 81 75 79 81 81 Baseline 2.5 2.6 2.8 2.8 2.6 ∆ to placebo -0.05 -0.1 -0.31 -0.12 p value 0.7501 0.5267 0.0414 0.4515 Figure 46t: Graph showing change from baseline in mPPT Lift a Book and Put it on the Shelf scores, full analysis set from week 4 of ent through week 52. Line with dark diamond represents placebo; line with open circle represents 45 mg bid, line with triangle represents 67.5 mg bid, line with grey diamond represents 90 mg bid, line with square represents 112.5 mg bid.

Figure 46u: Change from baseline in mPPT Lift a Book and Put it on the Shelf scores, full is set at 26 weeks.

Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 81 75 79 81 81 Baseline 2.6 2.4 2.6 2.6 2.7 ∆ to placebo 0.14 0.1 0.04 0.14 p value 0.2277 0.3649 0.6982 0.2057 Figure 46v: Change from baseline in mPPT Lift a Book and Put it on the She lf scores, full analysis set at 52 weeks.

Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 81 75 79 81 81 Baseline 2.6 2.4 2.6 2.6 2.7 ∆ to placebo 0.25 0.17 0.22 0.12 p value 0.0755 0.224 0.1116 0.3956 Figure 47a: Graph showing change from baseline in mPPT Put on and Remove a Jacket full analysis set from week 4 of treatment through week 52. Line with dark diamond represents placebo; line with open circle represents 45 mg bid, line with triangle represents 67.5 mg bid, line with grey diamond represents 90 mg bid, line with square represents 112.5 mg bid.

Figure 47b: Change from baseline in mPPT Put on and Remove a Jacket scores, full analysis set at 26 weeks.

Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 81 75 79 81 81 Baseline 2.2 2.1 2.3 2.3 2.2 ∆ to placebo -0.22 0.17 -0.01 -0.03 p value 0.1319 0.2355 0.9331 0.8307 Figure 47c: Change from ne in mPPT Put on and Remove a Jacket scores, full analysis set at 52 weeks.

Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 81 75 79 81 81 Baseline 2.2 2.1 2.3 2.3 2.2 ∆ to placebo 0.2 0.21 0.08 -0.18 p value 0.2306 0.1943 0.615 0.2614 Figure 47d: Graph g change from baseline in mPPT Pick up a Penny from the Floor full analysis set from week 4 of treatment through week 52. Line with dark diamond represents placebo; line with open circle represents 45 mg bid, line with triangle represents 67.5 mg bid, line with grey d represents 90 mg bid, line with square represents 112.5 mg bid.

Figure 47e: Change fr om baseline in mPPT Pick up a Penny from the Floor scores, full analysis set at 26 weeks. The table below provides the data and P-Values corresponding to Figure 47e. o 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 81 75 79 81 81 Baseline 2.6 2.7 2.8 2.6 2.7 ∆ to placebo -0.13 0.14 0.1 0.07 p value 0.2702 0.2404 0.3764 0.5559 Figure 47f: Change from baseline in mPPT Pick up a Penny from the Floor scores, full analysis set at 52 weeks. The table below provides the data and P-Values ponding to Figure 47f.

Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 81 75 79 81 81 Baseline 2.6 2.7 2.8 2.6 2.7 ∆ to placebo 0.04 0.09 0.14 0.07 p value 0.7523 0.5148 0.3007 0.5939 Figure 47g: Graph showing change from baseline in mPPT Turn 360 Degrees scores full analysis set from week 4 of treatment h week 52. Line with dark diamond represents placebo; line with open circle represents 45 mg bid, line with triangle represents 67.5 mg bid, line with grey diamond represents 90 mg bid, line with square represents 112.5 mg bid.

Figure 47h: Change from baseline in mPPT Turn 360 Degrees scores, full analysis set at 26 weeks.

The table below provides the data and P-Values corresponding to Figure 47h.

Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 81 75 79 81 81 Baseline 3.2 3.2 3.1 3 3.1 ∆ to placebo 0.12 -0.06 0.12 0.2 p value 0.557 0.7586 0.5746 0.3518 Figure 47i: Change from baseline in mPPT Turn 360 Degrees scores, full analysis set at 52 weeks.

The table below provides the data and P-Values corresponding to Figure 47i.

Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 81 75 79 81 81 Baseline 3.2 3.2 3.1 3 3.1 ∆ to placebo -0.12 -0.1 0.04 -0.08 p value 0.636 0.6733 0.8805 0.7413 Figure 47j: Graph showing ch ange from baseline in mPPT 50 Feet Walk scores full analysis set from week 4 of treatment through week 52. Line with dark d represents placebo; line with open circle represents 45 mg bid, line with triangle represents 67.5 mg bid, line with grey diamond represents 90 mg bid, line with square ents 112.5 mg bid.

Figures 47k: Change from baseline in mPPT 50 Feet Walk scores, full analysis set at 26 weeks. The table below es the data and P-Values corresponding to Figure 47k.

Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 81 75 79 81 81 Baseline 3.3 3.2 3.3 3.4 3.3 ∆ to placebo -0.02 0 -0.1 -0.17 p value 0.8367 0.9738 0.3331 0.0945 Figures 47l: Change from baseline in mPPT 50 Feet Walk scores, full analysis set at 52 weeks. Th e table below provides the data and P-Values corresponding to Figure 47l.

Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 81 75 79 81 81 Baseline 3.3 3.2 3.3 3.4 3.3 ∆ to placebo -0.03 0.15 -0.23 -0.15 p value 0.8302 0.3032 0.1087 0.3004 Figure 47m: graph showing change from baseline in mPPT Climb One Flight of Stairs scores full analysis set from week 4 of ent h week 52. Line with dark diamond represents placebo; line with open circle represents 45 mg bid, line with le represents 67.5 mg bid, line with grey diamond represents 90 mg bid, line with square represents 112.5 mg bid.

Figures 47n: Change from baseline in mPPT Climb One Flight of Stairs scores, full analysis set at 26 weeks. The table below provides the data and P-Values corresponding to Figure 47n.

Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 81 75 79 81 81 Baseline 2.6 2.6 2.7 2.6 2.8 ∆ to placebo 0.08 0.11 0.15 0.16 p value 0.5139 0.3671 0.2061 0.1912 Figures 47o: Change from baseline in mPP T Climb One Flight of Stairs scores, full analysis set at 52 weeks. The table below provides the data and P-Values corresponding to Figure 47o.

Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 81 75 79 81 81 Baseline 2.6 2.6 2.7 2.6 2.8 ∆ to placebo 0.26 0.13 0.08 0.11 p value 0.0896 0.4116 0.6043 0.4606 Figure 47p: Change from baseline in mPPT Climb One Flight of Stairs scores in pridopidine treated late stage HD patients with BL TFC < 7, at 52 weeks. The table below provides the data and P-Values corresponding to Figure 47p.

Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 19 16 25 25 23 Baseline 2.5 2.5 2.4 2.3 2.5 ∆ to placebo 0.19 -0.3 -0.26 -0.15 p value 0.5914 0.3609 0.4198 0.6539 Figure 47q: Change from baseline in mPPT Climb One Flight of Stairs scores in idine treated HD1 and HD2 patients, at 52 weeks. The table below provides the data and es corresponding to Figure 47q.

Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 62 59 54 56 58 Baseline 2.7 2.6 2.8 2.7 2.9 ∆ to placebo 0.27 0.3 0.22 0.23 p value 0.1076 0.0769 0.1955 0.1771 Figure 47r: Change from ne in mPPT Climb On e Flight of Stairs scores in pridopidine d HD1 patients, at 52 weeks. The table below provides the data and P-Values corresponding to Figure 47r.

Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 12 17 17 11 18 Baseline 2.7 3.2 2.7 2.9 3.2 ∆ to placebo -0.02 0.01 -0.06 0.18 p value 0.9539 0.9627 0.86 0.5277 Figure 47s: Change from baseline in mPPT Climb One Flight of Stairs scores in pridopidine d HD2 patients, at 52 weeks. The table below provides the data and P-Values corresponding to Figure Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 50 42 37 45 40 Baseline 2.7 2.3 2.9 2.7 2.8 ∆ to placebo 0.35 0.34 0.28 0.23 p value 0.0958 0.1189 0.1482 0.2731 Figure 47t: Change from baseline in mPPT Climb One Flight of Stairs scores in pridopidine treated late stage HD patients with BL TFC 0-6, at 52 weeks. The table below provides the data and P-Values ponding to Figure 47t.

Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 19 16 25 25 23 Baseline 2.5 2.5 2.4 2.3 2.5 ∆ to placebo 0.19 -0.3 -0.26 -0.15 p value 0.5914 0.3609 0.4198 0.6539 Figure 47u: Graph showing change from baseline in mPPT Climb Stairs (Flights Up and Down) scores full analysis set from week 4 of treatment through week 52. Line with dark diamond represents o; line with open circle represents 45 mg bid, line with triangle represents 67.5 mg bid, line with grey diamond represents 90 mg bid, line with square represents 112.5 mg bid. s 47v: Change from baseline in mPPT Climb Stairs (Flights Up and Down) scores, full analysis set at 26 weeks. The table below provides the data and P-Values corresponding to Figure 47v. o 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 81 75 79 81 81 Baseline 3.4 3.5 3.6 3.6 3.7 ∆ to placebo -0.03 -0.14 -0.08 -0.08 p value 0.8134 0.195 0.464 0.4872 Figures 47w: Change from baseline in mPPT Climb Sta irs (Flights Up and Down) scores, full analysis set at 52 weeks. The table below provides the data and P-Values corresponding to Figure 47w.

Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 81 75 79 81 81 Baseline 3.4 3.5 3.6 3.6 3.7 ∆ to placebo 0.17 0.04 -0.04 0.1 p value 0.3209 0.8251 0.8282 0.5759 Figure 47x: Change from baseline in mPPT Climb Stairs (Flights Up and Down) scores in late stage HD patients with BL TFC < 7, at 52 weeks. The table below provides the data and P-Values corresponding to Figure 47x.

Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 19 16 25 25 23 Baseline 3.2 3.1 3.4 3.5 3.1 ∆ to placebo 0.72 0.16 -0.36 0.31 p value 0.0915 0.6827 0.3305 0.4235 Figure 47y: Change from baseline in mPPT Climb Stairs (Flights Up and Down) scores in pridopidine treated HD1 and HD2 ts, at 52 weeks. The table below provides the data and PValues corresponding to Figure 47y.

Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid N 62 59 54 56 58 Baseline 3.5 3.6 3.7 3.6 3.9 ∆ to placebo -0.01 0.01 0.1 0.07 p value 0.9572 0.9614 0.5816 0.7161 DETAILED DESCRIPTION OF THE INVENTION This invention provides a method of maintaining functional capacity, improving functional capacity, or lessening the decline of functional capacity in a human patient in need thereof comprising periodically orally administering to the patient a pharmaceutical composition comprising pridopidine or a pharmaceutically acceptable salt thereof such that a dose of 90-225 mg of pridopidine is administered to the patient per day, so as to thereby in functional capacity, improve functional capacity, or lessening the decline of functional capacity in the human patient. In an embodiment, the method comprises maintaining onal capacity, improving functional capacity, or lessening the decline of functional capacity.

This invention provides a method of maintaining functional capacity, improving functional capacity, reducing the rate of decline of functional capacity, or slowing the rate of functional decline in a human patient in need thereof comprising periodically orally administering to the patient a ceutical composition comprising pridopidine or a pharmaceutically acceptable salt thereof such that a dose of 90-225 mg of pridopidine is administered to the patient per day, so as to y maintain functional capacity, improve functional capacity, reduce the rate of decline of onal capacity or slow the rate of onal decline in the human patient. In an embodiment, the method comprises maintaining functional capacity, improving functional capacity, or reducing the rate of decline of functional capacity.

In an embodiment, the method comprises maintaining function ty. In another embodiment, the method comprises improving functional capacity. In some embodiments, the functional capacity is maintained or improved, for at least 12 weeks, at least 20 weeks, at least 26 weeks, at least 52 weeks, at least 78 weeks, at least 3 years, or at least 5 years.

In another embodiment, the functional capacity is total functional capacity (TFC) measured by UHDRS-TFC and the human patient has an improvement of one (1) or more points in the UHDRS TFC. In some embodiments, the human patient has an improvement of one (1) or more points in the UHDRS TFC after 52 weeks of stration of pridopidine. In another ment, the rate of functional decline is less than one (1) point as measured by the UHDRS TFC after 52 weeks of stration of idine.

In one embodiment, the method comprises lessening the decline of functional capacity. In another embodiment, the method comprises lessening the decline of functional ty and (a) the pharmaceutical composition is administered for more than 26 weeks or (b) the human patient is afflicted with early stage HD. In one embodiment, the method comprises reducing the rate of e of onal capacity. In another embodiment, the method comprises reducing the rate of decline of functional ty and (a) the pharmaceutical composition is administered for more than 26 weeks or (b) the human patient is afflicted with early stage HD. In some embodiments, the method comprises ing functional decline. In some embodiments, the decline in functional capacity is lessened by or the rate of functional e is slowed for at least 20%, at least 30%, at least 40%, at least 50%, or at least 80%. In another embodiment, the rate of the decline in functional capacity is reduced for at least 12 weeks, at least 20 weeks, at least 26 weeks, at least 52 weeks, at least 78 weeks, at least 3 years, or at least 5 years. In another embodiment, rate of functional decline is slowed in functional capacity is reduced for at least 12 weeks, at least 20 weeks, at least 26 weeks, at least 52 weeks, at least 78 weeks, at least 3 years, or at least 5 years.

In one embodiment, the functional capacity is total functional ty (TFC). The total fu nctional ty may be measured by TFC. The total functional capacity may also be measured by the UHDRS Functional Assessment Scale (UHDRS-FAS). In an embodiment the functional capacity is maintained for at least 12 weeks, at least 20 weeks, at least 26 weeks, at least 52 weeks, or at least 78 weeks.

In an embodiment, the human patient has no deterioration of functional ty. In other embodiments, the human patient has no deterioration of functional capacity for at least 52 weeks.

The invention onally provides a method of g the clinical progression of HD in a human patient comprising periodically orally administering to the patient afflicted with HD a ceutical composition sing pridopidine or a pharmaceutically acceptable salt thereof such that a dose of 90-225 mg of pridopidine is administered to the patient per day, so as to thereby slow the clinical progression of HD in the patient.

In an embodiment, the clinical progression of HD is measured by total functional capacity. In one embodiment, the clinical progression of HD is slowed by at least 20%, at least 30%, at least 50%, at least 80%, or between 20% and 90%. In another embodiment, the clinical progression of HD is slowed for at least 12 weeks, at least 20 weeks, at least 26 weeks, at least 52 weeks, at least 3 years, or at least 5 years. In a further embodiment, the total functional capacity is measured by the UHDRS-TFC.

This invention also provides a method of reducing functional decline as measured by UHDRS Total Functional Capacity, in a human patient in need thereof comprising periodically orally administering to the human patient a ceutical composition sing pridopidine or a pharmaceutically acceptable salt thereof such that a dose of 90-225 mg of pridopidine is administered to the patient per day, so as to thereby reduce functional decline in the human patient, wherein the human patient is afflicted with HD and has a baseline TFC score of 11-13.

In an embodiment, functional decline is measured by UHDRS-TFC. In another embodiment, the method comprises reducing functional decline for at least 12 weeks, at least 20 weeks, at least 26 weeks, at least 52 weeks, at least 78 weeks, at least 3 years, or at least 5 years.

This invention also provides a method of maintaining, improving, or lessening the decline of, a human patient’s ability to perform activities of daily living, comprising periodically orally stering to the human patient in need thereof a pharmaceutical composition comprising pridopidine or a pharmaceutically acceptable salt thereof such that a dose of 90-225 mg of pridopidine is administered to the patient per day, so as to thereby maintain, improve, or lessen the decline of the human patient’s ability to perform activities of daily living.

This invention also provides a method of ining, ing, or reducing the rate of decline of, a human patient’s ability to perform activities of daily living, comprising periodically orally administering to the human patient in need thereof a pharmaceutical composition comprising pridopidine or a pharmaceutically acceptable salt thereof such that a dose of 90-225 mg of pridopidine is stered to the t per day, so as to thereby maintain, improve, or reduce the rate of decline of the human patient’s y to perform ties of daily living.

In one embodiment, the human patient’s y to perform activities of daily living is maintained, improved, or the decline is lessened over a period of at least 12 weeks, at least 20 weeks, at least 26 weeks, at least 52 weeks, or at least 78 weeks. In one embodiment, the human patient’s ability to perform activities of daily living is maintained, improved, or the rate of decline is reduced for at least 12 weeks, at least 20 weeks, at least 26 weeks, at least 52 weeks, or at least 78 weeks. In another embodiment, the method comprises maintaining the human patient’s ability to m activities of daily living. In an ment, the ability to perform activities of daily living is measured by the Activities of Daily Living (ADL) domain of the TFC.

The invention also provides a method of maintaining, improving, or lessening the decline of, a human patient’s y to manage finances, sing periodically orally stering to the human patient in need thereof a pharmaceutical composition comprising pridopidine or a ceutically acceptable salt thereof such that a dose of 90-225 mg of pridopidine is stered to the patient per day, so as to thereby maintain, improve, or lessen the rate of decline of the human patient’s ability to manage finances.

The invention also provides a method of maintaining, improving, or reducing the rate of decline of, a human patient’s ability to manage finances, comprising periodically orally administering to the human patient in need thereof a pharmaceutical composition comprising pridopidine or a pharmaceutically acceptable salt thereof such that a dose of 90-225 mg of pridopidine is administered to the patient per day, so as to thereby maintain, e, or reduce the rate of decline of the human patient’s ability to manage finances.

In another embodiment, administering further maintains, improves, or lessens the decline of the human patient’s ability to manage finances. In an embodiment, the human patient’s ability to manage finances is maintained, improved, or the e of is lessened for at least 12 weeks, at least 20 weeks, at least 26 weeks, at least 52 weeks, or at least 78 weeks. In another embodiment, administering further maintains, improves, or reduces the rate of decline of the human patient’s ability to manage finances.

In an embodiment, the human patient’s y to manage finances is maintained, improved, or the rate of decline is reduced for at least 12 weeks, at least 20 weeks, at least 26 weeks, at least 52 weeks, or at least 78 weeks. In one embodiment, the method comprises maintaining the human patient’s ability to manage finances. In another embodiment, the method comprises improving the human patient’s ability to manage finances. In some embodiments, the ability to manage es is measured by the Managing Finances domain of the TFC.

In one embodiment, administering further maintains, es, or s the rate of decline of the human patient’s ability to perform domestic chores. In another embodiment, administering further ins, improves, or lessens the e of the human patient’s ability to perform domestic chores.

The invention also provides a method of maintaining, improving, or lessening the decline of, a human patient’s ability to perform domestic chores, comprising periodically orally administering to the human t in need therefore a pharmaceutical composition comprising pridopidine or a pharmaceutically acceptable salt thereof such that a dose of 90-225 mg of pridopidine is administered to the patient per day, so as to thereby maintain, improve, or lessen the decline of the human patient’s y to perform domestic chores.

The invention also es a method of maintaining, improving, or reducing the rate of decline of, a human patient’s ability to perform domestic chores, comprising periodically orally administering to the human patient in need therefore a pharmaceutical composition comprising pridopidine or a pharmaceutically able salt thereof such that a dose of 90-225 mg of pridopidine is administered to the t per day, so as to thereby maintain, improve, or reduce the rate of decline of the human patient’s ability to perform domestic chores.

In an embodiment, the ability to perform domestic chores is measured by the Domestic Chores domain of the UHDRS TFC. In another embodiment, the human patient’s ability to perform domestic chores is maintained or improved for at least 12 weeks, at least 20 weeks, at least 26 weeks, at least 52 weeks, or at least 78 weeks. In another embodiment, the method comprises maintaining the human patient’s ability to perform domestic . In one ment, the method comprises improving the human patient’s ability to m domestic chores. In another ment, the human patient’s ability to perform domestic chores is maintained or improved, or the rate of decline is reduced for at least 12 weeks, at least 20 weeks, at least 26 weeks, at least 52 weeks, or at least 78 weeks. In a further embodiment, the human patient’s ability to perform ic chores is maintained or improved, or the decline is lessened for at least 12 weeks, at least 20 weeks, at least 26 weeks, at least 52 weeks, or at least 78 weeks.

In one embodiment, administering further maintains, improves, or reduces the rate of decline of, the care level of the human patient. In another embodiment, administering further maintains, improves, or lessens the decline of, the care level of the human patient.

The invention also provides, a method of maintaining, improving, or lessening the decline of, a human patient’s care level, comprising periodically orally administering to the human patient in need thereof a pharmaceutical composition comprising pridopidine such that a dose of 90-225 mg of pridopidine is stered to the patient per day, so as to thereby in, improve, or lessen the decline of the human patient’s care level.

The invention also provides, a method of ining, improving, or reducing the rate of decline of, a human patient’s care level, comprising periodically orally administering to the human t in need thereof a pharmaceutical composition comprising pridopidine such that a dose of 90-225 mg of pridopidine is administered to the t per day, so as to thereby maintain, improve, or reduce the rate of decline of the human patient’s care level.

In an embodiment, the care level is measured by the Care level domain of the TFC. In another embodiment, the human patient’s care level is maintained, improved, or the rate of decline is reduced for at least 12 weeks, at least 20 weeks, at least 26 weeks, at least 52 weeks, or at least 78 weeks. In another embodiment, the human patient’s care level is maintained, improved, or the decline of is lessened for at least 12 weeks, at least 20 weeks, at least 26 weeks, at least 52 weeks, or at least 78 weeks. In r embodiment, the method ses maintaining the care level of the human patient.

In one embodiment, a dose of 135-225 mg of pridopidine is administered to the patient per day. In another embodiment, a dose of 180-225 mg of pridopidine is administered to the t per day. In another embodiment, a dose of 90 mg of pridopidine is administered to the patient per day, a dose of 135 mg of pridopidine is administered to the patient per day, a dose of 180 mg of pridopidine is administered to the patient per day, or a dose of 225 mg of pridopidine is stered to the t per day. In another embodiment, a dose of 135 mg of pridopidine is administered to the t per day, a dose of 180 mg of idine is administered to the patient per day, or a dose of 225 mg of pridopidine is stered to the patient per day. In another embodiment, a dose of 180 mg of pridopidine is administered to the patient per day, or a dose of 225 mg of pridopidine is administered to the patient per day. In another embodiment, a dose of 90 mg of pridopidine is administered to the patient per day, a dose of 135 mg of pridopidine is administered to the patient per day, or a dose of 180 mg of pridopidine is stered to the patient per day. In another embodiment, a dose of 90 mg of pridopidine is administered to the patient per day. In another embodiment, a dose of 90 mg of pridopidine is administered to the patient per day in unit doses of 45 mg twice per day. In another embodiment, a dose of 135 mg of pridopidine is administered to the patient per day. In another embodiment, a dose of 135 mg of pridopidine is stered to the patient per day in unit doses of 67.5 mg twice per day. In r embodiment, a dose of 180 mg of pridopidine is administered to the patient per day. In another embodiment, a dose of 180 mg of pridopidine is administered to the patient per day in unit doses of 90 mg twice per day. In another embodiment, a dose of 225 mg of pridopidine is administered to the patient per day. In another ment, a dose of 225 mg of pridopidine is stered to the patient per day in unit doses of 112.5 mg twice per day.

The invention further provides a method of reducing dystonia or maintaining a level of dystonia in a human t in need thereof comprising ically orally administering to the patient a pharmaceutical composition comprising pridopidine such that a dose of 90-225 mg of idine is administered to the patient per day, so as to thereby reduce dystonia or maintain a level of dystonia in the human patient.

In one embodiment, ia is measured by the UHDRS TMS Dystonia score. In another embodiment, the level of dystonia in the human patient is reduced or maintained for at least 12 weeks, at least 20 weeks, at least 26 weeks, at least 52 weeks, or at least 78 weeks.

In some embodiment the dystonia is limb ia.

The invention also provides a method of treating limb dystonia in a human patient in need thereof comprising periodically orally administering to the patient a pharmaceutical composition comprising pridopidine such that a dose of 90-225 mg of pridopidine is administered to the patient per day, so as to thereby treat the limb dystonia in the human patient. In many embodiments, (a) the pharmaceutical composition is administered for more than 26 weeks or (b) a titration dose of an amount different from the intended dose is administered for a period of time at the start of the periodic stration or (c) the human patient is afflicted with early stage HD In an embodiment, a dose of 135-225 mg of pridopidine is administered to the patient per day. In another embodiment, a dose of 135 mg of pridopidine is administered to the t per day, a dose of 135 mg of pridopidine is administered to the patient per day, a dose of 180 mg of idine is administered to the patient per day, or a dose of 225 mg of pridopidine is stered to the patient per day. In another embodiment, a dose of 135 mg of pridopidine is administered to the patient per day, a dose of 180 mg of pridopidine is administered to the patient per day, or a dose of 225 mg of pridopidine is administered to the patient per day. In another embodiment, a dose of 180 mg of pridopidine is administered to the t per day, or a dose of 225 mg of pridopidine is administered to the patient per day. In another embodiment, a dose of 180 mg of idine is administered to the patient per day. In another embodiment, a dose of 90 mg of pridopidine is administered to the patient per day.

In another embodiment, the pharmaceutical composition is administered for at least 12 weeks, at least weeks, at least 26 weeks, more than 26 weeks, at least 52 weeks, at least 54 weeks, at least 78 weeks, at least 104 weeks or more. In another embodiment, the treating limb ia comprises preventing the slowing, the reduction in amplitude, or the impairment of the human patient’s finger g y and/or preventing the slowing or the irregular performance of the Pronate-Supinate Hands test in the human patient.

This invention also provides a method of preventing the slowing, the reduction in ude, or the impairment of the human patient’s finger tapping ability and/or preventing the slowing or the irregular performance of the Pronate-Supinate Hands test in a human HD patient comprising periodically orally administering to the patient a pharmaceutical composition comprising pridopidine such that a dose of 90-225 mg of pridopidine is stered to the t per day so as to thereby prevent the slowing, the reduction in amplitude, or the impairment of the human patient’s finger tapping ability and/or prevent the slowing or the irregular performance of the Pronate-Supinate Hands test in the human In another embodiment, the treating limb dystonia comprises preventing the impairment of the human patient’s finger g ability and/or preventing the slowing or the irregular performance of the Q- Motor: Pro-Sup-Frequency-MN-Hand (Hz) test. In another ment, the treating comprises improving the human patient’s Q-Motor tap speed frequency. In another embodiment, the treating comprises improving the human patient’s Q-Motor tap speed inter onset interval (IOI).

The invention further provides a method of improving or maintaining, a human patient’s gait and balance comprising periodically orally administering to the human patient in need thereof a pharmaceutical composition comprising pridopidine such that a dose of 90-225 mg of pridopidine is administered to the patient per day, so as to thereby improve or maintain, a human patient’s gait and balance.

In one embodiment, a dose of 90 mg, 135 mg, 180 mg, or 225 mg of pridopidine is administered to the patient per day. In r embodiment, a dose of 90 mg of pridopidine is administered to the t per day. In another embodiment, a dose of 180 mg of pridopidine is administered to the patient per day. In r embodiment, a dose of 135 mg or 180 mg of pridopidine is administered to the patient per day.

Additionally provided is a method of improving, maintaining, or lessening the decline of, a human patient’s gait and balance comprising periodically orally administering to the human patient in need thereof a pharmaceutical composition comprising pridopidine such that a dose of 90 mg of pridopidine is administered to the patient per day, so as to thereby improve, maintain, or lessen the decline of, a human patient’s gait and balance.

Also provided is a method of improving, ining, or slowing the decline of, a human patient’s gait and e comprising periodically orally administering to the human patient in need thereof a pharmaceutical composition comprising pridopidine such that a dose of 90 mg of idine is administered to the patient per day, so as to thereby improve, in, or slow the decline of, a human patient’s gait and balance.

In an embodiment, the human patient’s gait and balance is measured by the UHDRS gait and balance score. In some embodiments, the human t’s gait and e is improved or maintained or the decline is lessened for at least 12 weeks, at least 20 weeks, at least 26 weeks, at least 52 weeks, or at least 78 weeks.

In an embodiment, the human patient’s gait and balance is measured by the UHDRS gait and balance score. In some embodiments, the human patient’s gait and balance is ed or maintained or the decline is slowed for at least 12 weeks, at least 20 weeks, at least 26 weeks, at least 52 weeks, or at least 78 weeks.

The invention also provides a method of improving or maintaining, a human patient’s independence comprising periodically orally administering to the human patient in need thereof a pharmaceutical composition comprising pridopidine such that a dose of 90-225 mg of pridopidine is administered to the patient per day, so as to thereby improve or maintain a human patient’s independence.

In one embodiment, a dose of 90 mg, 135 mg, 180 mg, or 225 mg of idine is administered to the patient per day. In another embodiment, a dose of 90 mg of pridopidine is administered to the t per day. In another embodiment, a dose of 225 mg of pridopidine is administered to the t per The invention also provides a method of improving, maintaining, or ing the decline of, a human patient’s independence comprising periodically orally administering to the human t in need thereof a pharmaceutical composition comprising pridopidine such that a dose of 90 mg of pridopidine is administered to the patient per day, so as to thereby improve, maintain, or lessen the decline of, a human t’s independence.

The invention also provides a method of ing, maintaining, or slowing the decline of, a human patient’s independence sing periodically orally administering to the human patient in need thereof a pharmaceutical composition comprising pridopidine such that a dose of 90 mg of pridopidine is administered to the patient per day, so as to thereby improve, maintain, or slow the decline of, a human patient’s independence.

In an embodiment, the human patient’s independence is measured by the UHDRS Independence score.

In some embodiments, the human patient’s independence is improved or maintained, or the decline is slowed for at least 12 weeks, at least 20 weeks, at least 26 weeks, at least 52 weeks, or at least 78 weeks. In one embodiment, the human patient’s independence is improved or maintained, or the decline is lessened for at least 12 weeks, at least 20 weeks, at least 26 weeks, at least 52 weeks, or at least 78 weeks.

The invention also provides a method of improving or maintaining a human patient’s ive domains comprising periodically orally administering to the human patient in need f a pharmaceutical composition sing pridopidine such that a dose of 90-225 mg of pridopidine is administered to the patient per day, so as to thereby improve or maintain the human patient’s cognitive domains. A patient’s cognitive domains may also be the t’s cognitive mance across a variety of s In one embodiment, a dose of 90-180 mg of pridopidine is administered to the patient per day. In another embodiment, a dose of 90 mg, 135 mg, or 180 mg of pridopidine is administered to the patient per day. In another embodiment, a dose of 90 mg, or 180 mg of pridopidine is administered to the patient per day.

Further ed is a method of improving, maintaining, or lessening the decline of, a human t’s cognitive domains comprising periodically orally administering to the human patient in need thereof a pharmaceutical composition comprising pridopidine such that a dose of 90 mg of pridopidine is administered to the patient per day, so as to thereby improve, maintain, or lessen the decline of, a human patient’s cognitive domains. Cognitive domains may be understood as cognitive performance across a variety of domains.

Further provided is a method of improving, maintaining, or slowing the decline of, a human patient’s cognitive domains comprising periodically orally administering to the human t in need thereof a pharmaceutical composition comprising pridopidine such that a dose of 90 mg of idine is stered to the patient per day, so as to thereby e, maintain, or slow the decline of, a human patient’s cognitive s. Cognitive domains may be tood as cognitive performance across a variety of domains.

The human patient’s cognitive domains may be measured, for example, by the cognitive assessment battery (CAB). The human patient’s cognitive s may also be measured by the s Verbal Learning Test – Revised (HVLT-R). The human t’s cognitive domains may additionally be measured by the Paced Tapping test, the Montreal Cognitive Assessment (MoCA) scale or the Symbol Digit Modalities Test (SDMT). The human patient’s cognitive domains may additionally be measured by trail making test B (TMT-B). In one embodiment, the human patient’s cognitive domains is maintained or improved, or the decline is slowed for at least 12 weeks, at least 20 weeks, at least 26 weeks, at least 52 weeks, or at least 78 weeks. In some ments, slowing the decline of a human patient’s cognitive domains comprises g the rate of cognitive decline. In an embodiment, the human patient’s cognitive domains is maintained or improved, or the decline is lessened for at least 12 weeks, at least 20 weeks, at least 26 weeks, at least 52 weeks, or at least 78 weeks.

The invention also provides a method of reducing the severity of the ned or intermittent muscle contractions associated with dystonia in a human patient in need thereof comprising periodically orally administering to the patient a pharmaceutical composition comprising pridopidine such that a dose of 90-225 mg of pridopidine is stered to the patient per day, so as to thereby reduce the ty of the sustained or intermittent muscle contractions associated with dystonia in the human patient. In many embodiments, (a) the pharmaceutical composition is administered for more than 26 weeks or (b) a titration dose of an amount different from the intended dose is administered for a period of time at the start of the periodic administration and/or (c) the human patient is afflicted with early stage HD.

The ty of the sustained or ittent muscle contractions associated with dystonia in a human patient may be measured by, for e, the UHDRS TMS Dystonia score.

Further provided is a method of improving or maintaining motor ability in a human patient in need thereof comprising periodically orally administering to the patient a pharmaceutical composition comprising pridopidine such that a dose of 90-225 mg of pridopidine is stered to the patient per day, so as to thereby maintain or improve motor ability in the human patient.

The motor y may be measured, for example, by the UHDRS TMS score, the UHDRS TMS score excluding chorea or UHDRS TMS score excluding dystonia.

In an embodiment, a dose of 90 mg, 135 mg, 180 mg, or 225 mg of pridopidine is administered to the patient per day. In another embodiment, a dose of 90 mg of pridopidine is administered to the t per day. In another embodiment, a dose of 180 mg of pridopidine is administered to the patient per day. In another embodiment, the motor ability is maintained or improved for at least 12 weeks, at least weeks, at least 26 weeks, at least 52 weeks, at least 78 weeks.

The ion also provides a method of reducing or maintaining the level of chorea in a human patient in need thereof sing periodically orally administering to the patient a pharmaceutical composition comprising pridopidine such that a dose of 90-225 mg of idine is administered to the patient per day, so as to thereby reduce or maintain the level of chorea in a human patient.

In one embodiment, a dose of 90 mg, 135 mg, 180 mg, or 225 mg of pridopidine is administered to the patient per day. In another embodiment, a dose of 90 mg of pridopidine is administered to the patient per day. In another embodiment, a dose of 135 mg or 180 mg of pridopidine is administered to the patient per day. The level of chorea may also be reduced.

The ion also provides a method of reducing, maintaining, or lessening the increase of, chorea in a human patient in need thereof comprising ically orally administering to the patient a pharmaceutical composition sing pridopidine such that a dose of 90 mg of pridopidine is administered to the patient per day, so as to thereby , maintain, or lessen the increase of, chorea in a human patient.

The invention also provides a method of reducing, maintaining, or slowing the increase of, chorea in a human patient in need thereof comprising periodically orally administering to the patient a pharmaceutical composition comprising pridopidine such that a dose of 90 mg of pridopidine is stered to the patient per day, so as to thereby reduce, maintain, or slow the increase of, chorea in a human patient.

In one embodiment, the chorea in the human patient is improved, or maintained, or the increase is slowed for at least 12 weeks, at least 20 weeks, at least 26 weeks, at least 52 weeks, or at least 78 weeks. In an ment, the chorea in the human t is improved, or maintained, or the increase is lessened for at least 12 weeks, at least 20 weeks, at least 26 weeks, at least 52 weeks, or at least 78 weeks. The human patient’s chorea may be measured by the UHDRS TMS chorea score.

The ion further provides a method of improving, maintaining, reducing or lessening the decline of a human patient’s behavior and/or psychiatric state comprising periodically orally administering to the human patient in need thereof a pharmaceutical ition comprising idine such that a dose of 90-225 mg of pridopidine is administered to the patient per day, so as to thereby improve, maintain, reduce, or lessen the decline of the human patient’s behavior and/or psychiatric state.

In one embodiment, the method comprises maintaining a human patient’s behavior and/or psychiatric state. In another embodiment, the method ses improving the human patient’s behavior and/or psychiatric state. In another ment, the human patient’s behavior and/or psychiatric state is improved, maintained or the decline is reduced or lessened for at least 12 weeks, at least 20 weeks, at least 26 weeks, at least 52 weeks, or at least 78 weeks.

The human patient’s behavior and/or psychiatric state may be measured by the Problem Behaviors Assessment (PBA) total score. The human patient’s behavior and/or psychiatric state may also be ed by the Problem Behaviors ment-short form (PBA-s). The human patient’s or and/or psychiatric state may also be measured by the Problem Behaviors Assessment for depressed mood. The human patient’s behavior and/or psychiatric state may also be measured by the Problem Behaviors Assessment for bility. The human patient’s behavior and/or psychiatric state may also be measured by the Problem Behaviors Assessment for lack of initiative or apathy. The human patient’s behavior and/or psychiatric state may also be measured by the Problem Behaviors Assessment short form apathy sub-item. The human patient’s behavior and/or psychiatric state may also be measured by the Apathy Evaluation Scale (AES). The human patient’s behavior and/or psychiatric state may be measured by the Problem Behaviors Assessment for obsessivecompulsiveness.

The human patient’s behavior and/or psychiatric state may also be measured by the Problem ors Assessment for disoriented behavior. In some embodiments, the human t’s behavior and/or psychiatric state is measured by the Problem Behaviors ment short form apathy sub-item or the Problem Behaviors Assessment-short form ).

The invention also provides a method of reducing or maintaining a human patient’s involuntary nts comprising periodically orally administering to the human patient in need thereof a pharmaceutical composition sing pridopidine such that a dose of 90-225 mg of pridopidine is administered to the patient per day, so as to thereby reduce or maintain a human patient’s ntary movements.

In one embodiment, the human patient’s involuntary movements are reduced or maintained for at least 12 weeks, at least 20 weeks, at least 26 weeks, at least 52 weeks, or at least 78 weeks. The patient’s involuntary movements may be measured by UHDRS TMS Involuntary Movements score.

The invention further provides method of improving or maintaining a human patient’s mobility comprising periodically orally administering to the human patient in need thereof a pharmaceutical composition comprising pridopidine such that a dose of 90-225 mg of pridopidine is administered to the patient per day, so as to thereby improve or maintain the human patient’s mobility.

In one embodiment, the human patient’s mobility is improved, or maintained for at least 12 weeks, at least 20 weeks, at least 26 weeks, at least 52 weeks, or at least 78 weeks. The human patient’s ty may be measured by the Timed Up and Go Test. The human t’s mobility may also be measured by the Walk-12 Total Score. The human patient’s mobility may further be measured by the patient’s g ability.

This invention also es a method of ing or maintaining a human patient’s ability to perform physical tasks comprising periodically orally administering to the human patient in need thereof a ceutical composition comprising pridopidine such that a dose of 90-225 mg of pridopidine is administered to the patient per day, so as to thereby improve or in the human patient’s ability to perform physical tasks.

In one embodiment, the human patient’s y to perform physical tasks is improved, or ined for at least 12 weeks, at least 20 weeks, at least 26 weeks, at least 52 weeks or at least 78 weeks. In one embodiment, the human patient’s ability to perform physical tasks is measured by the modified physical performance test (mPPT).

In another embodiment, the human patient’s ability to perform physical tasks is measured by the mPPT stairs climbing test. In another embodiment, the human patient’s ability to perform physical tasks is measured by the mPPT total score. In another embodiment, the human patient’s ability to perform physical tasks is measured by the mPPT standing static balance test. In another embodiment, the human patient’s y to perform physical tasks is measured by the mPPT chair rise test. In another embodiment, the human patient’s ability to perform al tasks is ed by the mPPT lift a book and put it on a shelf test. In r embodiment, the human patient’s ability to perform physical tasks is measured by the mPPT put on and remove a jacket test. In another embodiment, the human patient’s ability to perform physical tasks is measured by the mPPT pick up a penny from floor test. In another embodiment, the human patient’s ability to perform al tasks is measured by the mPPT turn 360 degrees test. In another ment, the human patient’s ability to perform physical tasks is measured by the mPPT 50 feet walk test. In another embodiment, the human patient’s ability to m al tasks is measured by the mPPT climb one flight of stairs test. In another embodiment, the human patient’s y to perform physical tasks is ed by the mPPT climb stairs test (flights up and down).

The invention also provides, a method of ing or maintaining a human patient’s quality of life comprising periodically orally administering to the patient a pharmaceutical composition comprising pridopidine such that a dose of 90-225 mg of idine is administered to the patient per day, so as to thereby improve or maintain the human patient’s quality of life.

In one embodiment, the human patient’s quality of life is improved, or maintained for at least 12 weeks, at least 20 weeks, at least 26 weeks, at least 52 weeks, or at least 78 weeks. In another embodiment, the human patient’s quality of life is maintained. In another embodiment, the human patient’s quality of life is measured by the Huntington’s Disease Quality of Life (HD-QoL) score.

The invention further es a method of reducing the natural decline in the total onal capacity of a HD patient, comprising periodically orally administering to the patient a pharmaceutical composition comprising pridopidine such that a dose of 90-225 mg of pridopidine is administered to the patient per day, so as to thereby reduce the natural decline in the total functional capacity in the human patient. In one ment, the natural decline is reduced by 20-70%, 30%-60%, or 35%-45%.

In another ment, the natural decline is reduced by 20%, 30%, 40%, 50%, 60% or 70%. In an embodiment, the natural decline is lessened by 0.16-0.56, 0.24-0.48, 0.28-0.36 points per year as measured by the UHDRS-TFC. In another embodiment, the l decline is lessened by 0.16, 0.24, 0.32, 0.4, 0.48 or 0.56 points per year as measured by UHDRS-TFC.

Neurofilament light proteins (NfL) may be used as a biomarker of neurodegeneration in HD patients (Byrne 2017). NfL trations in plasma were found to increase with advancing HD disease.

Thus, NfL concentrations in plasma of HD patients may e a means for assessing and predicting neural damage in patients with HD (Byrne 2017). Additionally, results suggest that NfL in the blood could provide a reliable estimate of the concentration of NfL in the CSF (Byrne 2017).

The invention further provides a method of maintaining, reducing, or lessening the increase of, the concentration of neurofilament light protein in a HD patient, comprising periodically orally administering to the patient a pharmaceutical composition comprising pridopidine such that a dose of 90-225 mg of pridopidine is administered to the t per day, so as to thereby maintain, decrease, or lessen the increase of, the concentration of neurofilament light protein in the human patient. In one embodiment, the increase of the concentration of ilament light n is lessened in the human t. In another embodiment, the concentration of neurofilament light n is maintained or decreased in the human patient.

The invention further provides a method of predicting clinical responsiveness to pridopidine therapy in a subject afflicted with HD, the method comprising administering an amount of pridopidine and evaluating the amount of a neurofilament light protein in the subject, so as to thereby t al responsiveness to pridopidine.

In one embodiment, the method further comprising predicting positive clinical responsiveness to pridopidine if the amount of the neurofilament light protein is decreased in the subject after stration of pridopidine compared to baseline. In one embodiment, the method further comprising predicting positive clinical siveness to pridopidine if the amount of the neurofilament light n is maintained in the subject after administration of pridopidine relative to ne. In r embodiment, the method further comprising predicting positive clinical responsiveness to pridopidine if the amount of the neurofilament light protein is increased in the subject after administration of pridopidine. Baseline, in this paragraph, is the amount of the neurofilament light protein prior to administration of pridopidine.

In one embodiment, the subject is identified as a pridopidine responder if amount of the biomarker is higher than a reference value. In another embodiment, the subject is identified as a pridopidine responder if amount of the biomarker is lower than a reference value.

In another embodiment, if the subject is identified as a pridopidine responder, the t is thereafter administered a pharmaceutical composition comprising pridopidine.

In one embodiment, a dose of 90 mg, 135 mg, 180 mg or 225 mg of pridopidine is administered to the patient per day. In another embodiment, a dose of 90 mg, 135 mg, or 225 mg of pridopidine is administered to the patient per day. In another embodiment, a dose of 90 mg of pridopidine is stered to the patient per day. In another embodiment, a dose of 135 mg of idine is administered to the patient per day. In another embodiment, a dose of 180 mg of pridopidine is administered to the patient per day. In another embodiment, a dose of 225 mg of pridopidine is administered to the patient per day.

In an embodiment, the human patient is afflicted with HD.

In some embodiments, a unit dose of the pharmaceutical composition contains 45 mg, 67.5 mg, 90 mg, or 112.5 mg of pridopidine.

In an embodiment, the pharmaceutical composition is administered twice per day. In another embodiment, an equal amount of the pharmaceutical ition is administered at each administration. In an embodiment, the two doses are administered at least 6 hours apart, at least 7 hours, at least 8 hours, at least 9 hours, at least 10 hours, at least 11 hours apart. In some embodiments, the pharmaceutical ition is administered for at least 12 weeks, at least 20 weeks, at least 26 weeks, more than 26 weeks, at least 52 weeks, or at least 78 weeks.

In one embodiment, the t has a UHDRS-TMS score ≥25 before beginning treatment. In another embodiment, the t has a UHDRS-IS (UHDRS-Independence Scale) score below 90% before beginning treatment. In r embodiment, the patient has greater than or equal to 36 CAG repeats in the huntingtin gene. In another embodiment, the human patient has greater than 44 CAG repeats in the huntingtin gene. In another embodiment, the human patient has less than 44 CAG repeats in the huntingtin gene. In another embodiment, the human patient is afflicted with early stage HD. In another embodiment, the human patient has a baseline TFC score which is greater than or equal to 9. In r embodiment, the human patient has a ne TFC score which is greater than or equal to 7.

In another embodiment, the human patient has a baseline TFC score of 11-13. In another embodiment, the human patient has a ne TFC score of 7-10. In another embodiment, the human patient has a baseline TFC score of 0-6. In r embodiment, the human patient is afflicted with HD and has a baseline TMS score which is in the least severe r of the l population of patients afflicted with HD. In another embodiment, the human pati ent is ted with HD and has a baseline TMS score which is in the two least severe quarters of the overall population of patients afflicted with HD.

In another embodiment, the human patient is afflicted with HD and has a baseline TMS score which is in the three least severe quarters of the overall population of patients afflicted with HD. In another embodiment, the human patient is afflicted with HD and has a baseline TMS score which is in the three least severe quarters of the overall population of patients afflicted with HD or a baseline TFC score which is greater than or equal to 9. In another ment, the human patient is afflicted with HD and has a baseline TMS score which is in the three least severe quarters of the overall population of ts afflicted with HD or a baseline TFC score which is greater than or equal to 9 or less than 44 CAG repeats in the huntingtin gene. In another embodiment, the human patient is afflicted with HD and has a baseline TMS score which is in the two least severe rs of the overall population of patients afflicted with gton’s disease. In another embodiment, the human patient is afflicted with HD and has a baseline TFC score which is greater than or equal to 9 or greater than 44 CAG repeats in the huntingtin gene. In another embodiment, the human patient is afflicted with HD and has a baseline TMS score which is in the three least severe quarters of the overall population of patients ted with HD or less than 44 CAG repeats in the huntingtin gene. In r embodiment, the human patient is afflicted with HD and has a baseline TFC score which is greater than or equal to 9 or a baseline TMS score which is in the three least severe quarters of the overall population of patients afflicted with HD.

In one embodiment, the pridopidine is pridopidine hydrochloride.

In an ment, a titration dose of an amount different from the intended dose is administered for a period of time at the start of the periodic administration. In some embodiments, the titration dose is half the amount of the intended dose. In another embodiment, the titration dose is administered in one administration per day and the intended dose is administered in two administrations per day. In one embodiment, the titration dose is administered for 7-21 or 7-14 days prior to the administration of the ed dose. In another embodiment, the titration dose is administered for 7 days, 14 days, or 21 days prior to the administration of the intended dose. The titration dose is preferably administered for fourteen days prior to the administration of the intended dose.

In an embodiment, the method further comprises no worsening of the human patient’s other HD ms compared to baseline. In an embodiment, the method further comprises no worsening of another m of HD in comparison to a human patient not administered pridopidine. In another embodiment, the symptoms are not worsened for at least 12 weeks, at least 20 weeks, at least 26 weeks, at least 52 weeks or at least 78 weeks.

Provided herein is a ceutical composition comprising pridopidine for use in maintaining functional capacity in a human patient n the pharmaceutical ition is to be periodically orally administered to the patient such that a dose of 90-225 mg of pridopidine is to be administered to the patient per day. In some embodiments functional capacity includes ADL.

Provided herein is use of an amount of pridopidine in the manufacture of a medicament maintaining functional capacity in a human patient wherein the medicament is formulated for periodic oral administration to the patient such that a dose of 90-225 mg of pridopidine is to be administered to the t per day. In some embodiments functional capacity includes ADL.

Provided herein is a ceutical composition comprising pridopidine for use in slowing the clinical progression of HD as measured by total functional capacity in a human patient n the pharmaceutical composition is to be periodically orally administered to the t such that a dose of 90-225 mg of pridopidine is to be administered to the t per day.

Provided herein is a use of an amount of pridopidine in the manufacture of a medicament for slowing the clinical ssion of HD as measured by total functional capacity in a human patient wherein the medicament is ated for periodic oral administration to the patient such that a dose of 90-225 mg of pridopidine is to be administered to the patient per day.

Provided herein is a pharmaceutical composition comprising pridopidine for use in ining a human patient’s y to perform activities of daily living in a human patient n the pharmaceutical composition is to be periodically orally administered to the t such that a dose of 90-225 mg of pridopidine is to be administered to the patient per day.

Provided herein is use of an amount of pridopidine in the manufacture of a medicament for use in maintaining a human patient’s ability to perform activities of daily living in a human patient wherein the medicament is formulated for periodic oral administration to the patient such that a dose of 90-225 mg of pridopidine is to be administered to the patient per day.

Provided herein is a pharmaceutical composition comprising pridopidine for use in reducing ia or maintaining a level of dystonia in a human patient wherein the pharmaceutical composition is to be periodically orally administered to the patient such that a dose of 90-225 mg of pridopidine is to be administered to the patient per day. In some embodiments dystonia includes limb ia.

Provided herein is use of an amount of pridopidine in the manufacture of a medicament for use in reducing dystonia or maintaining a level of dystonia in a human patient wherein the medicament is formulated for periodic oral administration to the patient such that a dose of 90-225 mg of pridopidine is to be stered to the patient per day. In some embodiments dystonia es limb dystonia.

Provided herein is a ceutical composition comprising pridopidine for use in treating limb dystonia in a human patient wherein the pharmaceutical composition is to be periodically orally administered to the patient such that a dose of 90-225 mg of pridopidine is to be administered to the patient per day.

Provided herein is use of an amount of pridopidine in the manufacture of a medicament for use in treating limb dystonia in a human patient wherein the medicament is formulated for periodic oral stration to the patient such that a dose of 90-225 mg of pridopidine is to be administered to the patient per day.

Provided herein is a ceutical composition sing pridopidine for use in improving or ining gait and balance in a human patient wherein the pharmaceutical composition is to be periodically orally administered to the patient such that a dose of 90-225 mg of pridopidine is to be administered to the patient per day. In some embodiments the administration slows the decline of a patients gait and balance.

Provided herein is use of an amount of pridopidine in the manufacture of a medicament for use in improving or maintaining, a human patient’s gait and balance in a human patient wherein the ment is formulated for periodic oral administration to the patient such that a dose of 90-225 mg of pridopidine is to be administered to the patient per day. In some embodiments the administration slows the decline of a patients gait and balance.

Provided herein is a pharmaceutical composition comprising pridopidine for use in improving, maintaining, or slowing the e of gait and balance in a human t wherein the pharmaceutical composition is to be periodically orally administered to the patient such that a dose of 90 mg of pridopidine is to be administered to the patient per day. In some embodiments the administration slows the e of a patients gait and balance.

Provided herein is use of an amount of pridopidine in the manufacture of a medicament for use in improving, maintaining, or slowing the e of, a human patient’s gait and balance in a human t wherein the medicament is formulated for ic oral administration to the patient such that a dose of 90 mg of pridopidine is to be administered to the patient per day. In some embodiments the administration slows the e of a patients gait and balance.

Provided herein is a pharmaceutical composition comprising pridopidine for use in improving or maintaining ndence in a human patient wherein the pharmaceutical composition is to be periodically orally administered to the patient such that a dose of 90-225 mg of pridopidine is to be administered to the patient per day.

Provided herein is use of an amount of pridopidine in the manufacture of a ment for use in improving or maintaining, a human patient’s independence wherein the medicament is formulated for periodic oral administration to the patient such that a dose of 90-225 mg of pridopidine is to be administered to the patient per day.

Provided herein is a pharmaceutical composition comprising pridopidine for use in improving or maintaining or slowing the decline of a human patient's independence wherein the ceutical ition is to be periodically orally administered to the patient such that a dose of 90 mg of pridopidine is to be administered to the patient per day.

Provided herein is use of an amount of pridopidine in the manufacture of a medicament for use in improving or maintaining, or slowing the decline of a human patient’s independence n the ment is formulated for periodic oral stration to the patient such that a dose of 90 mg of pridopidine is to be stered to the patient per day.

Provided herein is a pharmaceutical composition comprising pridopidine for use in improving or maintaining a human patient’s cognitive domains wherein the pharmaceutical composition is to be periodically orally administered to the patient such that a dose of 90-225 mg of pridopidine is to be administered to the patient per day.

Provided herein is use of an amount of pridopidine in the manufacture of a medicament for use in improving or maintaining a human patient’s cognitive domains wherein the medicament is formulated for periodic oral administration to the patient such that a dose of 90 mg of pridopidine is to be administered to the patient per day.

Provided herein is a pharmaceutical composition comprising pridopidine for use in improving or maintaining or slowing the decline of a human patient’s cognitive domains wherein the pharmaceutical composition is to be periodically orally administered to the t such that a dose of 90 mg of pridopidine is to be administered to the patient per day.

Provided herein is use of an amount of pridopidine in the manufacture of a medicament for use in improving or maintaining or slowing the decline of a human patient’s cognitive s wherein the medicament is formulated for periodic oral administration to the patient such that a dose of 90-225 mg of pridopidine is to be administered to the patient per day.

Provided herein is a pharmaceutical composition comprising idine for use in reducing the ty of the sustained or ittent muscle contractions ated with dystonia in a human patient wherein the pharmaceutical composition is to be ically orally administered to the t such that a dose of 90-225 mg of idine is to be administered to the patient per day.

Provided herein is use of an amount of pridopidine in the manufacture of a medicament for use in reducing the severity of the sustained or intermittent muscle contractions associated with dystonia in a human patient wherein the medicament is formulated for periodic oral administration to the patient such that a dose of 90-225 mg of pridopidine is to be stered to the patient per day. ed herein is a pharmaceutical composition comprising pridopidine for use in improving or maintaining motor ability in a human patient wherein the pharmaceutical composition is to be periodically orally administered to the patient such that a dose of 90-225 mg of pridopidine is to be administered to the patient per day.

Provided herein is use of an amount of pridopidine in the manufacture of a medicament for use in improving or maintaining motor ability in a human patient wherein the medicament is formulated for periodic oral stration to the t such that a dose of 90-225 mg of pridopidine is to be administered to the patient per day.

Provided herein is use of an amount of idine in the manufacture of a medicament for use in reducing or maintaining the level of chorea in a human patient wherein the medicament is formulated for periodic oral administration to the patient such that a dose of 90-225 mg of pridopidine is to be stered to the patient per day.

Provided herein is a pharmaceutical composition comprising pridopidine for use in reducing or maintaining or slowing the increase of chorea in a human patient wherein the pharmaceutical composition is to be periodically orally administered to the patient such that a dose of 90 mg of pridopidine is to be administered to the patient per day.

Provided herein is use of an amount of pridopidine in the manufacture of a medicament for use in reducing or maintaining or slowing the increase of chorea in a human patient wherein the medicament is ated for periodic oral administration to the patient such that a dose of 90 mg of pridopidine is to be administered to the patient per day.

Provided herein is a pharmaceutical ition comprising pridopidine for use in ing or maintaining a human patient’s or and/or psychiatric state wherein the ceutical composition is to be periodically orally administered to the patient such that a dose of 90-225 mg of pridopidine is to be administered to the patient per day.

Provided herein is use of an amount of idine in the manufacture of a medicament for use in improving or maintaining a human patient’s behavior and/or psychiatric state wherein the ment is formulated for periodic oral administration to the patient such that a dose of 90-225 mg of pridopidine is to be administered to the patient per day.

Provided herein is a pharmaceutical composition comprising pridopidine for use in reducing or maintaining a human patient’s involuntary movements wherein the pharmaceutical composition is to be periodically orally administered to the patient such that a dose of 90-225 mg of idine is to be administered to the t per day.

Provided herein is use of an amount of idine in the manufacture of a medicament for use in reducing or maintaining a human patient’s involuntary nts wherein the medicament is formulated for periodic oral administration to the patient such that a dose of 90-225 mg of pridopidine is to be administered to the patient per day.

Provided herein is a pharmaceutical composition comprising pridopidine for use in improving or maintaining a human patient’s mobility n the pharmaceutical composition is to be periodically orally administered to the patient such that a dose of 90-225 mg of pridopidine is to be administered to the patient per day.

Provided herein is use of an amount of pridopidine in the cture of a medicament for use in improving or maintaining a human patient’s mobility wherein the medicament is formulated for periodic oral administration to the patient such that a dose of 90-225 mg of idine is to be administered to the patient per day.

The subject invention also provides a package comprising: a) a pharmaceutical composition comprising pridopidine; and b) instructions for use of the pharmaceutical composition according to the methods of the present ion.

Provided herein is a pharmaceutical ition comprising pridopidine for use in improving or ining a human patient’s ability to perform physical tasks wherein the pharmaceutical composition is to be periodically orally administered to the patient such that a dose of 90-225 mg of pridopidine is to be administered to the patient per day.

Provided herein is a use of an amount of pridopidine in the manufacture of a medicament for use in improving or maintaining a human patient’s ability to perform physical tasks n the medicament is formulated for periodic oral administration to the patient such that a dose of 90-225 mg of pridopidine is to be administered to the patient per day.

The invention also provides, a method of maintaining or improving total onal capacity, in a human t afflicted with HD comprising periodically orally administering to the patient a ceutical composition comprising pridopidine such that a dose of 90 or 180 mg of pridopidine is administered to the patient per day, so as to thereby maintain functional capacity, or improve total onal capacity, in the human patient as measured by the UHDRS-TFC for at least 26 or 52 weeks.

In one embodiment, a dose of 90 mg of pridopidine is administered to the patient per day. In r embodiment, the human t has a baseline TFC score of 11-13. In another embodiment, the human patient has a baseline TFC score of 7-10. In another embodiment, the human patient has a baseline TMS score which is in the two least severe quarters of the overall population of patients afflicted with HD. In another embodiment, the human patient has a baseline TMS score which is in the three least severe quarters of the overall tion of patients afflicted with HD. In another embodiment, the human patient has less than 44 CAG repeats in the Huntingtin gene.

The invention also provides, a method of ining, or improving a human patient’s ability to m activities of daily living, sing periodically orally administering to the patient a pharmaceutical composition sing pridopidine such that a dose of 90 or 180 mg of pridopidine is administered to the patient per day, so as to thereby maintain, or improve the human patient’s ability to perform ties of daily living as measured by Activities of Daily Living domain of the UHDRS- TFC for at least 26 or 52 weeks, n the human patient is afflicted with HD.

In one embodiment, administering further maintains or improves the human patient’s ability to manage finances as measured by measured by the Managing Finances domain of the UHDRS-TFC for at least 26 or 52 weeks.

The invention also provides, a method of maintaining, or improving a human patient’s ability to manage finances, comprising periodically orally administering to the patient a pharmaceutical composition comprising pridopidine such that a dose of 90 or 180 mg of pridopidine is administered to the patient per day, so as to thereby maintain, or improve the human patient’s ability to manage finances as measured by Managing Finances domain of the UHDRS-TFC for at least 26 or 52 weeks, wherein the human patient is afflicted with HD.

In one embodiment, a dose of 90 mg of pridopidine is administered to the patient per day. In r embodiment, the human patient has a baseline UHDRS-TFC score of 11-13. In another embodiment, the human patient has a baseline UHDRS-TFC score of 7-10. In another ment, the human patient has a baseline TMS score which is in the two least severe quarters of the overall tion of patients afflicted with HD. In another embodiment, the human patient has a ne TMS score which is in the three least severe quarters of the overall population of patients afflicted with HD.

The invention also provides, a method of ining, improving, or the rate of decline of, a human patient’s ability to perform ic chores as measured by the Domestic Chores domain of the UHDRS-TFC, comprising periodically orally administering to the patient a pharmaceutical composition comprising pridopidine such that a dose of 90 mg of pridopidine is administered to the patient per day, so as to thereby maintain, improve, or reduce the rate of decline of the human t’s ability to perform domestic chores, wherein the human patient is afflicted with HD and has a baseline TFC score of 11-13.

The invention also provides, a method of maintaining, improving, or lessening the decline of, a human patient’s ability to m domestic chores as measured by the Domestic Chores domain of the UHDRS TFC, sing periodically orally administering to the patient a pharmaceutical composition comprising pridopidine such that a dose of 90 mg of idine is administered to the patient per day, so as to thereby maintain, improve, or lessen the decline of the human patient’s ability to perform domestic chores, wherein the human patient is afflicted with HD and has a baseline TFC score of 11-13.

The invention also provides, a method of maintaining, ing, or ng the rate of e of, a human patient’s care level as measured by the Care Level of the UHDRS TFC, comprising periodically orally administering to the patient a pharmaceutical composition sing pridopidine such that a dose of 90 mg of pridopidine is administered to the patient per day, so as to thereby maintain, improve, or reduce the rate of decline of the human patient’s care level, n the human patient is ted with HD and has a baseline TFC score of 11-13.

The invention also provides, a method of maintaining, improving, or lessening the e of, a human patient’s care level as measured by the Care Level of the UHDRS TFC, comprising periodically orally stering to the patient a pharmaceutical composition comprising pridopidine such that a dose of 90 mg of pridopidine is administered to the patient per day, so as to thereby maintain, improve, or lessen the decline of, the human patient’s care level, wherein the human patient is afflicted with HD and has a baseline TFC score of 11-13.

The invention also provides, a method of improving or maintaining, a human patient’s gait and balance comprising periodically orally administering to the patient a ceutical composition comprising pridopidine such that a dose of 90 mg of pridopidine is administered to the patient per day, so as to thereby improve, or maintain, a human patient’s gait and balance as measured by the UHDRS gait and balance score for at least 52 weeks, wherein the human patient is afflicted with HD and has a baseline TFC score of 11-13.

The invention also provides, a method of reducing dystonia or maintaining a level of dystonia in a human patient afflicted with HD sing periodically orally administering to the patient a pharmaceutical composition comprising pridopidine such that a dose of 90 or 180 mg of pridopidine is stered to the patient per day, so as to thereby reduce or maintain a level of dystonia as measured by the UHDRS TMS Dystonia score and the human t has a baseline TFC score of 11-13.

The invention also provides, a method of improving, maintaining, or slowing the decline of, a human patient’s independence comprising periodically orally administering to the patient a pharmaceutical ition comprising pridopidine such that a dose of 90 mg of pridopidine is administered to the patient per day, so as to thereby improve, maintain, or slow the decline of, a human patient’s independence as measured by the UHDRS Independence Score for at least 26 weeks, wherein the human patient is ted with HD.

The ion also es, a method of improving, maintaining, or lessening the decline of, a human patient’s independence comprising periodically orally administering to the patient a pharmaceutical composition sing pridopidine such that a dose of 90 mg of pridopidine is administered to the patient per day, so as to thereby improve, maintain, or lessen the decline of, a human patient’s independence as measured by the UHDRS Independence Score for at least 26 weeks, wherein the human patient is afflicted with HD.

In one embodiment, the human patient has a baseline TFC score of 11-13. In another embodiment, the human patient has a baseline TFC score of greater than or equal to 7.

The invention also provides, a method of preventing the slowing, the reduction in amplitude, or the impairment of the human patient’s finger tapping ability in a human patient afflicted with HD comprising periodically orally administering to the patient a pharmaceutical composition comprising pridopidine such that a dose of 90 mg of pridopidine is administered to the patient per day, so as to thereby prevent the slowing, the ion in amplitude, or the impairment of the human patient’s finger tapping ability.

In one embodiment, the method further ses preventing the slowing or the irregular performance of the Pronate-Supinate Hands test in the human patient.

The invention also es, a method of improving or maintaining a human patient’s behavior and/or psychiatric state comprising periodically orally administering to the human patient afflicted with HD a pharmaceutical composition comprising pridopidine such that a dose of 90 or 180 mg of pridopidine is stered to the patient per day, so as to thereby e or maintain the human patient’s or and/or psychiatric state for at least 26 weeks or at least 52 weeks as measured by the Problem ors Assessment for irritability or for disoriented or.

In one ment, the human patient has a baseline TFC score of 0-6, the human patient’s behavior and/or psychiatric state is measured by the m Behaviors Assessment for irritability and the human t’s behavior and/or psychiatric state is improved or maintained for at least 52 weeks. In another embodiment, the human patient has a baseline TFC score of 11-13, the human patient’s behavior and/or psychiatric state is measured by the Problem Behaviors Assessment for disoriented behavior and the human patient’s behavior and/or psychiatric state is improved or maintained for at least 26 weeks.

The invention also provides, a method of maintaining: a) functional capacity in a human patient; b) a human patient’s y to perform activities of daily living; c) a human patient’s ability to manage finances; d) a human patient’s ability to perform domestic chores; e) the human patient’s care level; f) Dystonia in a human patient; g) a human patient’s Gait and balance; h) a human patient’s independence; i) a human patient’s cognitive domains; j) chorea in a human patient; k) a human patient’s behavior and/or psychiatric state; l) motor ability in a human patient; m) a human patient’s mobility; or n) a human patient’s ability to perform physical tasks; comprising periodically orally administering to the patient a pharmaceutical composition sing pridopidine such that a dose of 90-225 mg of pridopidine is administered to the patient per day.

The invention also provides, a method of improving: a) functional ty; b) a human t’s ability to perform ties of daily living; c) a human patient’s ability to manage finances; d) a human patient’s ability to perform domestic chores; e) a human patient’s care level; f) a human patient’s gait and balance; g) a human patient’s independence; h) a human patient’s cognitive s; i) motor ability in a human patient; j) chorea in a human patient; k) a human patient’s behavior and/or psychiatric state; l) a human patient’s mobility; or m) a human patient’s ability to perform physical tasks; comprising periodically orally administering to the patient a pharmaceutical composition comprising pridopidine such that a dose of 90-225 mg of pridopidine is stered to the patient per day.

The invention also provides, a method of reducing: a) ia in a human patient; b) a human patient’s involuntary movements; or c) the severity of the sustained or ittent muscle contractions associated with dystonia in a human patient, comprising periodically orally administering to the t a pharmaceutical composition comprising idine such that a dose of 90-225 mg of pridopidine is administered to the patient per day.

The invention also provides a method of reducing: a) the decline of functional capacity in a human patient; b) the rate of decline of a human patient’s ability to perform activities of daily living; c) the rate of decline of a human patient’s y to manage finances; d) the rate of decline of a human patient’s ability to perform domestic chores; e) the rate of decline of a human patient’s care level; or f) the decline of a human patient’s behavior and/or psychiatric state; comprising periodically orally stering to the t a pharmaceutical composition comprising pridopidine such that a dose of 90-225 mg of pridopidine is administered to the patient per day.

The invention also provides a method of ing: a) the decline of functional capacity in a human patient; b) the decline of a human patient’s ability to perform activities of daily living; c) the decline of a human patient’s ability to manage finances; d) the decline of a human patient’s ability to perform domestic chores; e) the decline of a human patient’s care level; or f) the decline of a human t’s behavior and/or psychiatric state; comprising periodically orally administering to the patient a pharmaceutical ition comprising pridopidine such that a dose of 90-225 mg of pridopidine is administered to the patient per day.

The invention also es a method of: a) slowing the e of, a human patient’s gait and balance; b) slowing the decline of, a human patient’s independence; or c) slowing the decline of, a human patient’s cognitive domains; comprising periodically orally administering to the patient a pharmaceutical composition comprising pridopidine such that a dose of 90 mg of pridopidine is stered to the patient per day.

The invention also provides a method of: a) lessening the decline of, a human patient’s gait and balance; b) lessening the e of, a human patient’s independence; or c) lessening the decline of, a human patient’s cognitive domains; comprising periodically orally administering to the patient a pharmaceutical composition comprising pridopidine such that a dose of 90 mg of pridopidine is administered to the t per day.

The invention also provides a method of: a) slowing the clinical progression of HD as measured by total functional ty in a human patient; or b) treating limb dystonia, preferably, wherein treating comprises i. preventing the slowing, the reduction in amplitude, or the impairment of the human patient’s finger tapping ability and preventing the g or irregular mance of the e-Supinate Hands test in the human patient; ii. preventing the slowing or the irregular mance of the Pronate-Supinate Hands test in the human patient; iii. improving the human patient’s Q-motor tap speed frequency; or iv. improving the human patient’s Q-motor tap speed inter onset interval (IOI); comprising periodically orally administering to the patient a pharmaceutical composition comprising idine such that a dose of 90-225 mg of pridopidine is administered to the patient per day.

The invention further provides a pharmaceutical ition comprising pridopidine for use in (1) (a) maintaining functional capacity, ing functional ty, or lessening functional decline in a human patient in need thereof, (b) slowing the clinical progression of HD, (c) reducing dystonia or maintaining a level of dystonia in a human patient in need thereof, (d) treating limb ia in a human patient in need thereof, (e) preventing the slowing, the reduction in amplitude, or the impairment of the human patient’s finger tapping ability and/or preventing the slowing or the lar mance of the Pronate-Supinate Hands test, (f) improving or maintaining, a human patient’s gait and balance in a human patient in need thereof, (g) improving or maintaining, a human patient’s independence in a human patient in need thereof, (h) improving or maintaining a human t’s cognitive performance across a variety of s in a human patient in need thereof, (i) lessening the severity of the sustained or intermittent muscle contractions associated with dystonia in a human patient in need f, (j) improving or maintaining motor ability in a human patient in need thereof, (k) reducing or maintaining the level of chorea in a human patient in need thereof, (l) improving, maintaining, or lessening the decline of a human patient’s behavior and/or psychiatric state in a human patient in need thereof, (m) reducing or maintaining a human patient’s involuntary movements in a human patient in need thereof, (n) improving or maintaining a human patient’s mobility in a human patient in need thereof, (o) improving or maintaining a human patient’s ability to perform physical tasks, (p) ing or maintaining a human patient’s quality of life wherein the pharmaceutical composition is to be periodically orally administered to the t such that a dose of 90-225 mg of pridopidine is to be administered to the patient per day, or (2) (a) improving, maintaining, or lessening the decline of gait and balance in a human patient in need thereof (b) ing, maintaining, or lessening the e of, a human patient’s ndence in a human patient in need thereof, (c) improving, maintaining, or lessening the decline of, a human patient’s ive performance across a variety of domains in a human patient in need thereof, (d) reducing, maintaining, or lessening the increase of, chorea, in a human patient in need thereof, wherein the ceutical composition is to be periodically orally stered to the patient such that a dose of 90mg of pridopidine is to be administered to the patient per day.

The invention also provides the use of an amount of pridopidine in the manufacture of a medicament for (1) (a) maintaining functional capacity, improving functional capacity, or lessening functional decline in a human patient in need thereof, (b) slowing the al progression of HD, (c) reducing dystonia or maintaining a level of dystonia in a human patient in need thereof, (d) treating limb dystonia in a human patient in need thereof, (e) preventing the slowing, the reduction in amplitude, or the impairment of the human t’s finger tapping ability and/or preventing the slowing or the lar performance of the Pronate-Supinate Hands test, (f) improving or maintaining, a human patient’s gait and e in a human patient in need thereof, (g) improving or maintaining, a human patient’s independence in a human patient in need thereof, (h) improving or maintaining a human patient’s cognitive performance across a variety of domains in a human patient in need f, (i) ing the severity of the sustained or intermittent muscle contractions associated with dystonia in a human t in need thereof, (j) improving or maintaining motor ability in a human t in need thereof, (k) reducing or maintaining the level of chorea in a human patient in need thereof, (l) improving, maintaining, or lessening the decline of a human patient’s or and/or psychiatric state in a human t in need thereof, (m) reducing or maintaining a human patient’s involuntary movements in a human patient in need thereof, (n) improving or maintaining a human patient’s mobility in a human patient in need thereof, (o) improving or maintaining a human patient’s ability to perform al tasks, (p) improving or maintaining a human patient’s quality of life wherein the pharmaceutical composition is to be periodically orally administered to the patient such that a dose of 90-225 mg of pridopidine is to be administered to the patient per day, or (2) (a) improving, maintaining, or lessening the decline of gait and balance in a human patient in need thereof (b) improving, maintaining, or lessening the decline of, a human patient’s independence in a human patient in need thereof, (c) improving, maintaining, or lessening the decline of, a human patient’s ive performance across a variety of domains in a human patient in need thereof, (d) reducing, maintaining, or lessening the increase of, chorea, in a human patient in need f wherein the medicament is formulated for ic oral administration to the patient such that a dose of 90mg of pridopidine is to be administered to the patient per day.

The invention additionally provides the use of an amount of pridopidine for (1) (a) maintaining functional capacity, improving functional capacity, or lessening functional decline in a human patient in need thereof, (b) slowing the clinical progression of HD, (c) reducing dystonia or maintaining a level of dystonia in a human patient in need f, (d) treating limb dystonia in a human patient in need f, (e) preventing the slowing, the reduction in amplitude, or the impairment of the human patient’s finger tapping y and/or ting the slowing or the irregular performance of the Pronate-Supinate Hands test, (f) improving or maintaining, a human patient’s gait and balance in a human patient in need thereof, (g) improving or maintaining, a human patient’s independence in a human patient in need thereof, (h) improving or maintaining a human patient’s cognitive performance across a variety of domains in a human patient in need thereof, (i) lessening the severity of the sustained or ittent muscle contractions ated with dystonia in a human patient in need f, (j) improving or maintaining motor ability in a human patient in need thereof, (k) reducing or ining the level of chorea in a human patient in need thereof, (l) improving, ining, or lessening the decline of a human patient’s behavior and/or psychiatric state in a human t in need thereof, (m) reducing or maintaining a human patient’s involuntary movements in a human patient in need thereof, (n) improving or maintaining a human patient’s mobility in a human patient in need thereof, (o) improving or maintaining a human patient’s ability to perform al tasks, (p) improving or maintaining a human patient’s y of life wherein the pharmaceutical composition is to be periodically orally administered to the patient such that a dose of 90-225 mg of pridopidine is to be stered to the patient per day, or (2) (a) ing, maintaining, or lessening the decline of gait and balance in a human patient in need f (b) improving, maintaining, or lessening the decline of, a human patient’s independence in a human patient in need thereof, (c) improving, maintaining, or lessening the decline of, a human patient’s ive performance across a variety of domains in a human patient in need f, (d) reducing, maintaining, or lessening the increase of, chorea, in a human patient in need thereof wherein the medicament is formulated for periodic oral administration to the patient such that a dose of 90mg of pridopidine is to be administered to the patient per day.

Each embodiment disclosed herein is contemplated as being applicable to each of the other disclosed embodiments. For instance, all combinations of the s elements described herein are within the scope of the invention. Additionally, the elements recited in the packaging and pharmaceutical composition embodiments can be used in the method and use embodiments described herein.

Terms As used herein, and unless stated otherwise, each of the following terms shall have the definition set forth below.

The articles “a”, “an” and “the” are non-limiting. For example, “the method” includes the broadest definition of the meaning of the phrase, which can be more than one method.

As used herein, “effective” as in an amount effective to achieve an end means the quantity of a component that is sufficient to yield an indicated therapeutic response without undue adverse side s (such as toxicity, irritation, or allergic se) commensurate with a reasonable benefit/risk ratio when used in the manner of this disclosure. For example, an amount effective to maintain functional capacity or lessen decline in functional capacity. The specific effective amount varies with such factors as the particular condition being treated, the physical condition of the patient, the type of mammal being treated, the duration of the treatment, the nature of concurrent therapy (if any), and the specific formulations employed and the structure of the compounds or its tives.

As used herein, to “treat” or "treating" encompasses, e.g., reducing a symptom, inducing inhibition, regression, or stasis of the disorder and/or disease. As used herein, ition” of disease progression or disease complication in a subject means preventing or reducing the disease progression and/or disease cation in the subject.

“Administering to the subject” or “administering to the (human) patient” means the giving of, dispensing of, or application of medicines, drugs, or remedies to a subject/patient to e, cure, or reduce the symptoms associated with a condition, e.g., a pathological condition. The administration can be periodic administration.

As used herein, “periodic administration” means repeated/recurrent administration separated by a period of time. The period of time between administrations is preferably consistent from time to time.

Periodic administration can e stration, e.g., once daily, twice daily, three times daily, four times daily, weekly, twice weekly, three times weekly, four times a week and so on, etc.

As used herein, “maintaining functional capacity in a human patient” means that the functional capacity score after a period of administration of pridopidine (“the after administration ) is unchanged compared to the human t’s functional capacity score immediately prior to the period of administration (“the baseline ). The after administration score is considered to be unchanged if the ence n the baseline score and the after administration score is not statistically significant. The functional capacity score can be measured as bed herein and includes subsets of the functional capacity score.

As used herein, “improving functional ty in a human patient” means that the functional ty score after a period of administration of pridopidine (“the after stration score”) is improved compared to the human patient’s functional capacity score immediately prior to the period of stration (“the baseline score”).

The functional capacity score of a human patient afflicted with HD can se over time. The rate of such decrease can be referred to as a rate of decline of the onal capacity score or a rate of decline of functional capacity or a rate of functional decline. For example, on average the rate of onal decline or the ion in TFC score is faster for early stage HD ts (TFC score 7-13) than for advanced stage patients (TFC score of <7) . On average the decline is about 0.8-1.2 points per year in early stage HD patients, less than 1/2 (about 0.2-0.3) point per year for patients with TFC 3-6; and less than 0.1 for patients with TFC 0-2 (Marder 2000). Therefore, TFC is most sensitive to change in the earlier stages of disease. The total functional capacity score can be measured as described herein and includes subsets of the functional capacity score. This decline may also be referred to as the l e or the untreated decline in functional capacity.

Accordingly, as used herein, “reducing the rate of decline of functional capacity”, “slowing the rate of functional e”, “reducing the rate of functional decline”, "decreased functional decline", or ‘slowing functional decline” means that the rate of decline of the functional capacity score after a period of administration of pridopidine (“the after administration score”) is slowed, reduced or decreased compared to the onal capacity score of a patient who has not received the same treatment with pridopidine.

As used herein, “lessening the decline of functional capacity” or “reducing the decline of functional capacity” means that the decrease in a functional capacity score in a patient after a period of administration of pridopidine is less than the decrease in the functional capacity score of a patient who has not received the same treatment with pridopidine over the same period.

As used herein, “maintaining a human patient’s ability to perform activities of daily living” means that the activities of daily living (ADL) score after a period of administration of pridopidine (“the after stration score”) is unchanged compared to the human patient’s activities of daily living score immediately prior to the period of administration (“the baseline score”). The after administration score is considered to be unchanged if the ence between the baseline score and the after administration score is not statistically significant. The activities of daily living score is a subset of the total functional capacity score and can be measured as described herein.

There are six basic ADLs: eating, g, dressing, toileting, erring (functional mobility) and continence. ADL is scored as follows: a patient requiring total care=0, a patient able to carry out gross tasks only=1, a patient having minimal impairment=2, a t with no impairment (normal)=3.

As used herein, “maintaining a human t’s ability to manage finances” means that the finances score after a period of administration of pridopidine (“the after administration score”) is unchanged compared to the human patient’s finances score immediately prior to the period of administration (“the baseline ). The after administration score is considered to be unchanged if the difference n the baseline score and the after administration score is not statistically significant. The finances score is a subset of the total functional capacity score and can be ed as described herein.

Finance is scored as follows: a patient unable to manage finances=0, a patient ing major assistance =1, a patient requiring minor assistance=2, a patient a patient requiring no assistance (normal)=3.

As used herein, “no worsening of other HD symptoms ed to baseline” means that the severity of each of the human patient’s HD symptoms after a period of administration of pridopidine is equal to or less than the severity of the symptom immediately prior to the start of the period of administration (baseline).

For each baseline score discussed above, in one embodiment, there is no stration of pridopidine to the patient prior to attainment of the baseline score. In another embodiment, an amount of pridopidine is administered to the patient prior to attainment of the baseline score. In a further embodiment, the amount of pridopidine administered to the patient prior to attainment of the ne score is less than or more than the amount of pridopidine administered to the patient after the attainment of the baseline score.

As used herein, an “amount” or “dose” of idine as measured in milligrams refers to the milligrams of pridopidine present in a preparation, regardless of the form of the ation. A “dose of 90 mg idine” means the amount of pridopidine acid in a preparation is 90 mg, regardless of the form of the preparation. Thus, when in the form of a salt, e.g. a pridopidine hydrochloride, the weight of the salt form necessary to provide a dose of 90 mg pridopidine would be greater than 90 mg due to the presence of the onal salt ion.

By any range disclosed herein, it is meant that all hundredth, tenth and integer unit amounts within the range are specifically disclosed as part of the invention. Thus, for example, 0.01 mg to 50 mg means that 0.02, 0.03 ... 0.09; 0.1; 0.2 ... 0.9; and 1, 2 ... 49 mg unit amounts are included as embodiments of this invention.

As used herein, “pridopidine” means pridopidine base or a pharmaceutically acceptable salt thereof, as well as derivatives, for example deuterium-enriched version of pridopidine and salts. Examples of deuterium-enriched pridopidine and salts and their s of preparation may be found in U.S.

Application Publication Nos. 2013-0197031, 2016-0166559 and 2016-0095847, the entire content of each of which is hereby incorporated by reference. In certain embodiments, pridopidine is a pharmaceutically acceptable salt, such as the HCl salt or tartrate salt. Preferably, in any embodiments of the invention as described herein, the pridopidine is in the form of its hydrochloride salt.

“Deuterium-enriched” means that the abundance of deuterium at any relevant site of the compound is more than the abundance of deuterium naturally occurring at that site in an amount of the compound.

The naturally occurring distribution of deuterium is about 0.0156%. Thus, in a rium-enriched” compound, the abundance of deuterium at any of its relevant sites is more than 0.0156% and can range from more than 0.0156% to 100%. Deuterium-enriched compounds may be obtained by exchanging hydrogen with ium or synthesizing the compound with ium-enriched ng materials ceutically Acceptable Salts The active nds for use according to the invention may be ed in any form le for the intended administration. Suitable forms include pharmaceutically (i.e. physiologically) acceptable salts, and pre- or prodrug forms of the compound of the invention.

Examples of pharmaceutically acceptable addition salts include, without tion, the non-toxic nic and organic acid addition salts such as the hloride, the hydrobromide, the L-tartrate, the nitrate, the perchlorate, the phosphate, the sulphate, the formate, the acetate, the aconate, the ascorbate, the benzenesulphonate, the benzoate, the cinnamate, the citrate, the embonate, the enantate, the fumarate, the glutamate, the ate, the lactate, the maleate, the malonate, the ate, the methanesulphonate, the naphthalenesulphonate, the phthalate, the salicylate, the sorbate, the stearate, the succinate, the tartrate, the toluene-p-sulphonate, and the like. Such salts may be formed by procedures well known and described in the art. ceutical Compositions While the compounds for use according to the invention may be administered in the form of the raw compound, it is preferred to introduce the active ingredients, optionally in the form of physiologically acceptable salts, in a pharmaceutical composition together with one or more adjuvants, excipients, carriers, buffers, diluents, and/or other customary ceutical auxiliaries.

In an embodiment, the invention provides pharmaceutical compositions comprising the active compounds or pharmaceutically acceptable salts or tives thereof, together with one or more pharmaceutically acceptable carriers therefore, and, optionally, other therapeutic and/or prophylactic ingredients know and used in the art. The carrier(s) must be “acceptable” in the sense of being compatible with the other ingredients of the formulation and not harmful to the recipient thereof.

The pharmaceutical composition of the invention may be administered by any convenient route, which suits the desired therapy. Preferred routes of administration include oral administration, in ular in , in capsule, in dragé, in powder, or in liquid form, and parenteral administration, in particular cutaneous, subcutaneous, intramuscular, or intravenous injection. The pharmaceutical composition of the invention can be manufactured by the skilled person by use of standard methods and conventional techniques appropriate to the desired formulation. When desired, compositions adapted to give sustained release of the active ingredient may be employed.

Further details on techniques for formulation and administration may be found in the latest edition of Remington’s Pharmaceutical Sciences (Mack hing Co., Easton, PA).

Listing of Abbreviations The following abbreviations are used throughout this application: ALT: e ransferase ; ADL: Activities of Daily Living; AR: gressive; AUC: area under the concentration-time curve; bid or b.i.d.: twice daily ; BL = Baseline; CAB: cognitive assessment battery; CAB HVLT-R: Cognitive ment Battery s Verbal ng Test, revised; CGI-C: al Global Impression of Change; CGI-S: Clinical Global Impression of Severity; CI: ence interval; CIBIC-Plus: Clinician’s Interview-based Impression of Change plus Caregiver Input; CIBIS: ian’s Interview-based Impression of Severity; CIOMS: Council for International Organizations of Medical Sciences; Cmax: maximum observed plasma drug concentration; CNS: central nervous system; CRF: case report form; CRO: contract research organization; CS: Compound Symmetry; C-SSRS: Columbia-Suicide Severity Rating Scale; CYP: cytochrome P450; DSM–IV TR: Diagnostic and Statistical Manual - Fourth n Text Revision; EM: extensive metabolizers; EU: European Union; FA: Functional Assessment; FAS: full analysis set; Freq: tapping frequency; GCP: Good Clinical Practice; GFV-C: grip force variability in the static phase; GGT: gamma-glutamyl transpeptidase; HART: Huntington’s disease ACR16 Randomized Trial; HCG: human chorionic gonadotropin; HD: Huntington’s disease; HD -QoL = Huntington’s disease y of Life; : HAD-CAB Hopkins Verbal Learning Test-Revised; ICH: International Conference on Harmonisation; IEC: Independent Ethics Committee; IOI: inter onset interval; IPI: inter peak interval; IRB: Institutional Review Board; IRT: interactive response logy; IS: Independence Score; ITI: inter tap interval; ITT: intent-to-treat; LSO: local safety officer; MAD: multiple ascending dose; MedDRA: Medical Dictionary for Regulatory Activities; MermaiHD: Multinational European Multicentre ACR16 study in HD; ML: m-Likelihood; mMS: Modified Motor Score; MoCA: Montreal cognitive assessment; MS: Multiple sclerosis; MTD: m tolerated dose; NMDA: N-methyl-D-aspartate; NOAEL: no observed adverse effect level; PBA-s: Problem Behaviors ment-Short form; PD: pharmacodynamic(s); PDS: Physical disability scale; PK: pharmacokinetic(s); PM: poor metabolizer; PPT: physical performance test; Qd: once daily; Q-Motor: Quantitative motor; QoL: Quality of life; QTcF: Fridericia-corrected QT interval; RBC: red blood cell; REML: Restricted Maximum-Likelihood; SAE: serious adverse event; SD: standard deviation; SDMT: symbol digit modalities test; SOC: system organ class; SOP: standard ing procedure; SUSAR: suspected cted serious adverse reaction; t½: half life; TC = telephone call; TD: tap duration; TF: g force; TFC: Total onal Capacity; TMS: Total Motor Score; TMS Involuntary Movements = TMS for performance of Domestic Chores and ia scores combined. TUG: timed up and go; UHDRS: Unified Huntington’s Disease Rating Scale; ULN: upper limit of the normal range; US: United States; WBC: white blood cell; WHO: World Health Organization; WHO: Drug World Health Organization (WHO) drug dictionary; ΔHR: change from baseline in heart rate; ΔQTcF: change from baseline in QTcF; ΔΔHR: placebo-corrected change from baseline in heart rate; Placebo-Controlled Huntington’s Disease; ΔΔQTcF: placebo-corrected change from baseline in QTcF, wk: week; EQ5D-5L European Quality of Life-5 Dimensions (5 levels).

Clinical Studies Sixteen (16) clinical studies have been completed with pridopidine, including 8 studies in healthy subjects (of which 1 study also included patients with schizophrenia), 1 study in patients with Parkinson’s disease, 2 studies in patients with schizophrenia (including the study mentioned , and 6 studies in patients with HD (including 1 open-label extension study). In on, a compassionate use program for pridopidine in patients with HD is ongoing in Europe, and an open- label, long term safety study is ongoing in the United States (US) and Canada. An overview of these studies are ted in International Publication No. WO 05229, the content of which is hereby incorporated by reference.

This invention will be better understood by reference to the Experimental Details which , but those skilled in the art will readily appreciate that the specific experiments detailed are only illustrative of the invention as described more fully in the claims which follow thereafter.

EXAMPLES Example 1: A Phase II, Dose -finding, Randomized, Parallel-Group, Double-Blind, Placebo- Controlled Study, Evaluating the Safety and Efficacy of Pridopidine 45 mg, 67.5 mg, 90 mg, and 112.5 mg Twice-Daily versus Placebo for Symptomatic Treatment in ts with HD (“PRIDE-HD”) The present study assessed the efficacy of pridopidine 45 mg to 112.5 mg twice daily (bid) on motor impairment in ts with HD over at least 52 weeks of treatment using the UHDRS TMS. The study also assessed the effect of at least 52 weeks of treatment with idine 45 mg bid to 112.5 mg bid on the Modified Physical Performance Test (mPPT). The study also ed the effect of at least 52 weeks of treatment with pridopidine 45 mg bid to 112.5 mg bid on UHDRS measures for total function capacity (TFC) and cognitive ment battery (CAB). The study also compared data from all patients to those obtained in HD subpopulations. The study also (i) evaluated the safety and tolerability of a range of pridopidine doses in patients with HD during at least 52 weeks of treatment, (ii) explored the pharmacokinetics (PK) of pridopidine in the study population and (iii) igated the relationship between exposure to pridopidine and outcome measures (e.g., clinical efficacy and toxicity parameters).

Study Design l Design and Study Schema This was a randomized, parallel-group, double blind, placebo controlled study that compared the cy and safety of pridopidine 45 mg, 67.5 mg, 90 mg, and 112.5 mg bid versus placebo in the treatment of motor impairment in HD. The administration of pridopidine to patients is summarized in Table 2. The study procedures and assessments are summarized in Table 3. A ed clinical procedure, including screening procedures and other procedures, is listed as Example 3 in U.S. Patent Application Publication No. US 2014/0378508 and International Publication No. the content of which are hereby incorporated by reference.

Primary and ary Variables and Endpoints The primary cy variable and endpoint for this study was change from ne in the UHDRS TMS (defined as the sum of all UHDRS motor domains ratings) at Week 26 or Week 52. The primary measure of motor ment is the UHDRS motor assessment section, which was administered by a trained examiner. The first part of the motor assessment ted of five TMS subscores, provided below. The sum total of all the 31 items is referred to as the Total Motor Score (TMS). The secondary efficacy variable and endpoint was change from baseline in the mPPT at Week 26 or Week 52.

The mPPT quantifies the patient’s performance in physical tasks (Brown 2000). It is a standardized 9- item test (standing static balance, chair rise, lift a book and put it on a shelf, put on and remove a jacket, pick up a penny from floor, turn 360 degrees, 50 feet walk, climb one flight of stairs, climb stairs test (flights up and down)). that measures the t’s performance on functional tasks. Both the speed and accuracy at which the ts complete the items are taken into account during scoring.

The maximum score of the test is 36, with higher scores indicating better performance. The Multiple Sclerosis Walking Scale (MSWS-12) was adapted to become a generic measure of walking and mobility and renamed the Walk-12.

Other Efficacy Variables and Endpoints: Other efficacy variables and endpoints for this study are as follows: Global Functional Scales: CIBIC-Plus global score as compared to baseline; Change from baseline in the PDS score; Change from baseline in UHDRS FA; CGIC as compared to baseline; Change from ne in UHDRS TFC; and Change from baseline in UHDRS IS.

Global/Functional Scales: Change from baseline in HD QoL; and Change from baseline in Walk-12 scale.

TMS Subscores: Change from baseline in hand movement score ed as the sum of UHDRS domains finger taps, pronate-supinate hands and luria [fist-hand-palm test]); Change from ne in Gait and balance score (defined as the sum of UHDRS domains gait, tandem g and retropulsion pull test); Change from baseline in UHDRS mMS (defined as the sum of UHDRS domains dysarthria, tongue protrusion, finger taps, pronate-supinate hands, luria, rigidity, bradykinesia, gait, tandem walking, retropulsion pull test); Change from baseline in UHDRS Chorea; Change from baseline in UHDRS Dystonia; and Responders, defined as patients with UHDRS TMS change from baseline ≤0.

Other Motor Assessments: Change from baseline in Q Motor measurements including digitomotography (speeded index finger tapping), dysdiadochomotography (pronation/supination hand tapping), manumotography and choreomotography (grip force and chorea analysis) and pedomotography (speeded foot tapping); and Change from baseline in the TUG test.

Cognitive/Psychiatric Assessments: Change from baseline in HD-CAB brief: SDMT, Emotion Recognition, Trail Making Test, HVLT-R, Paced Tapping at 3 Hz, OTS; and Change from baseline in PBA-s.

Safety Variables and Endpoints Safety variables and endpoints e the ing: AEs throughout the study; s from baseline in QTcF and other ECG parameters throughout the study; Clinical safety laboratory (clinical chemistry, hematology, and urinalysis) throughout study; Changes from ne C-SSRS throughout the study; Vital signs throughout the study. bility Variables and Endpoints Tolerability variables and endpoints include the following: the number (%) of patients who failed to complete the study; and the number (%) of ts who failed to complete the study due to AEs.

Pharmacokinetic Variables and Endpoints The primary PK measure was determination of plasma concentration of pridopidine. trations were also incorporated into a pridopidine population PK model and individual exposure for the study ts (Cmax and AUC) was calculated.

Study Drugs and Dosage: Pridopidine (as pridopidine hloride) was provided as a white hard n capsule, size 2 containing 45 mg pridopidine and a white hard gelatin capsule, size 4 containing 22.5 mg pridopidine. Placebo was presented as white hard gelatin capsules matching the 22.5 mg or 45 mg pridopidine capsules but containing no active ingredient, only the excipients (silicified microcrystalline cellulose and ium stearate).

Table 2: Dose Administration (Capsules were Administered Twice Daily to Give the Total Daily Dose) Titration Period Full Dose Period ent Week 1 Week 2 Week 3 Week 4a Weeks 4bto 52 idine 1 × 22.5 mg Pridopidine 1 × 22.5 mg 1 × 22.5 mg Pridopidine 1 × 22.5 mg 1 × 45 mg Pridopidine 45 mg bid 1 × 22.5 mg Placebo Pridopidine 1 × 22.5 mg Placebo Pridopidine 1 × 22.5 mg Placebo 1 × 45 mg Placebo 1 × 22.5 mg Placebo 1 × 45 mg Placebo 1 × 22.5 mg Placebo 1 × 45 mg Placebo (TDD = 45 mg) 1 × 45 mg Placebo (TDD = 45 mg) 1 × 45 mg Placebo (TDD = 90 mg) (TDD = 45 mg) (TDD = 45 mg) idine 1 × 22.5 mg Pridopidine 1 × 22.5 mg 1 × 45 mg Pridopidine 1 × 45 mg 1 × 22.5 mg 67.5 mg bid 1 × 22.5 mg Placebo Pridopidine 2 × 22.5 mg Placebo Pridopidine Pridopidine 1 × 45 mg o 1 × 22.5 mg Placebo 2 × 22.5 mg Placebo 1 × 45 mg Pridopidine (TDD = 45 mg) 1 × 45 mg Placebo (TDD = 90 mg) 1 × 45 mg Placebo (TDD = 45 mg) (TDD = 90 mg) (TDD = 135 mg) Pridopidine 1 × 22.5 mg Pridopidine 1 × 45 mg 1 × 45 mg Pridopidine 1 × 45 mg 2 × 45 mg Pridopidine 90 mg bid 1 × 22.5 mg Placebo Pridopidine 1 × 22.5 mg Pridopidine Pridopidine 1 × 22.5 mg Placebo 1 × 45 mg Placebo 2 × 22.5 mg Placebo 1 × 22.5 mg Placebo 1 × 22.5 mg (TDD = 45 mg) Pridopidine (TDD = 135 mg) (TDD = 180 mg) (TDD = 90 mg) 1 × 22.5 mg Placebo (TDD = 135 mg) Pridopidine 1 × 22.5 mg Pridopidine 1 × 45 mg 1 × 45 mg idine 1 × 45 mg 1 × 22.5 mg 112.5 mg bid 1 × 22.5 mg Placebo Pridopidine 1 × 22.5 mg Pridopidine Pridopidine Pridopidine 1 × 45 mg o 2 × 22.5 mg Placebo 1 × 22.5 mg Placebo 2 × 22.5 mg 2 × 45 mg Pridopidine Pridopidine (TDD = 45 mg) (TDD = 135 mg) (TDD = 90 mg) (TDD = 225 mg) (TDD = 180 mg) Placebo 2 × 22.5 mg Placebo 2 × 22.5 mg Placebo 2 × 22.5 mg Placebo 2 × 22.5 mg Placebo 1 × 22.5 mg Placebo 1 × 45 mg Placebo 1 × 45 mg Placebo 1 × 45 mg Placebo 1 × 45 mg o 2 × 45 mg Placebo TDD = total daily dose; a. Excluding Day 28; b. Day 28 only Table 3: Study ures and Assessments 106 106 mum-mm Procedures andAssessmeats first 26-Week Studv Period Second 26-week Studx Period IIIIHI\lSIt 280— 322- leanmumweeks 112:7 140:7 l82t7 273 :7 308 357 364t7 378:7 :10 5:10 Procedures and assessments Scree' week week week week week week week week EEEE 26 4‘ 4651 IAbbmnated PBA-s —CIBIC Plus ICGI-C EI—I I0 o L IEQSD-SL EM}—_IIE! IIEIJII mna--- III-ﬂ- I'H'IIEEIB mm-‘m-ﬁ antidepressants Inquuy‘ Revicwoftoluabilitytosmdy dmgpﬁortodosewcalation if ”liable IIIHHHIHHHIIHIHHII“ IIIHHIIIIIIIIIIIIIW IIIIIIIIIIIP IIIHHIIIIIIIIIIIII~ IIIHEHHHHHIIHIHHIFIIIHHHIIIIIIIIIIIIEg IIIIIIIIIIII IIIHHHHHHHHHHIHHI IIIIIIII MIBIEIEIIEBEEEIEIIIII “‘“Mw’mmm IEIﬂ-ﬂIﬂﬂﬂﬂﬂﬂIﬂIIIII IIIIIIIIIIIIIIIIIIIa Table 3 Legend a The procedures and ments for these visits (V0 and V4-12) may be performed over several days, as long as they are completed within the defined visit window. b. Inclusion/exclusion criteria should be met at screening and reviewed on Day 0 before the patient is randomized. c Electrolytes only. d Serum ncy test at screening (with urine test if required for confirmation); urine pregnancy test at subsequent time points. An indeterminate g for the serum pregnancy test should be checked twice (urine test) and the t ed to a gynecologist if required. e At screening, a single ECG will be performed. If there is evidence of a prolonged QTcF interval at screening (defined as a QTcF interval of >450 msec) then the ECG will be ed twice, and the mean of the 3 screening measurements will be used to determine whether or not the patient is suitable for inclusion in the study. f At the Baseline visit, the predose QTcF was ined by the average of 3 ECGs n 10 to 20 minutes of one another), each in triplicate (in total 9 recordings). A postdose ECG will be performed in triplicate 1 to 2 hours after first dosing. PK samples will be collected prior to and 1 to 2 hours after first dose administration at the site. When concomitant to ECG, PK samples are collected after the ECG recording. g One ECG performed in triplicate prior and 1 to 2 hours post afternoon dose. h ECG is al on Week 8, unless required by local regulations. It is to be performed at the investigator’s discretion where there are clinical circumstances that justify an additional ECG, eg, patients with a previous e of hypokalemia without QT prolongation. i On Week 52, a triplicate ECG and PK sample will be collected before the last study (morning) dose. j ECG is optional at the follow up visit, but should be performed for all patients with a previously observed cardiac concern and/or QTc change from baseline. k Including CAG analysis, cytochrome P450 2D6 status, genetic long QT syndrome (assessed only in patients experiencing QT prolongation ing study drug administration leading to study discontinuation), or any other genetic analyses related to pridopidine response or HD. l Evaluated in priority. m The safety telephone calls will include an abbreviated PBA-s (a subset of PBA questions on depressed mood, suicidal ideation, anxiety, irritability, loss of motivation and obsessive compulsive behaviors). n Including digitomotography (speeded index finger tapping), dysdiadochomotography (pronation/supination hand g), manumotography and choreomotography (grip force and chorea analysis) and pedomotography (speeded foot tapping). o es SDMT, Emotion recognition, Trail Making Test A+B, HVLT-R; Paced Tapping Test and p On Weeks 2, 12 and 20, PK samples will be collected 1 to 2 hours post afternoon dose. When concomitant to ECG, PK samples will be ted after the ECG recording. q On Weeks 4, 6 and 16, PK samples will be ted prior and 1 to 2 hours post afternoon dose.

When itant to ECG, PK samples will be collected after the ECG recording. r On the last study day (week 52), the study drug administration will take place on site, after the predose PK sample is obtained. s At the follow up visit, 1 PK sample will be collected. In case of SAE, an additional PK sampling should be aimed to be collected at the closest time to SAE. When concomitant to ECG, PK samples will be collected after the ECG ing. t This information will be collected as part of concomitant medication inquiry. u tion only. v Study adherence is reviewed during the TCs. w Every patient received 3 capsules twice daily (bid), ie, 3 capsules in the morning and 3 capsules in the afternoon (7 to 10 hours after the morning dose), during the whole study period. Study drug was not administered at Early Termination visit. At on-site visits, the afternoon dose were taken at the site. x Patients, who for safety or tolerability reasons have to stop study drug medication, are asked to ue in the study and follow the visit schedule as outlined without taking study drug.

Primary Efficacy Variable and Endpoint The UHDRS comprises a broad assessment of features associated with HD (Huntington Study Group 1996). It is a research tool which has been developed to provide a uniform assessment of the clinical features and course of HD. The TMS component of UHDRS comprises 31 assessments from the 15 items of the UHDRS, with each assessment rated on a 5-point scale from 0 (normal) to 4 (maximally abnormal).

Secondary Efficacy Variable and nt The secondary efficacy variable and nt, the Modified Physical mance Test (mPPT), quantifies the patient’s performance in physical tasks (Brown 2000). It is a standardized 9-item test that measures the patient’s performance on functional tasks. Assistive devices are permitted for the tasks that require a standing position (items 6 to 9). Both the speed and accuracy at which the patients complete the items were taken into account during scoring. The maximum score of the test is 36, with higher scores indicating better performance.

Other Efficacy les and Endpoints Clinician iew Based Impression of Change plus Caregiver Input The Plus (version ADCS-CGIC) was developed, validated, and is commonly used in studies of anti-dementia drugs in Alzheimer’s e (Joffres 2000). An ndent rater evaluated the patient’s overall disease severity prior to the initiation of idine or placebo. This assessment, known as the CIBIS, rates the t on a 7-point Likert scale from extremely severe HD to no symptoms of HD. At each subsequent visit in which the evaluation is performed, the CIBIC-Plus was administered by the same independent rater, but without knowledge of other endpoint assessments or the AEs experienced by the patient during the study (so as not to confound the rating of CIBIC as an efficacy measure or to unblind the study). The independent rater exclusively considers observations of the patient’s cognitive, functional, and behavioral performance obtained through interviewing the t and the caregiver. The rater then compared those findings to the ne assessment. The overall impression of change from baseline (CIBIC-Plus) is rated on a t scale: 1 = marked improvement; 2 = moderate improvement; 3 = minimal improvement; 4 = no change; 5 = minimal worsening; 6 = moderate worsening; 7 = marked ing; all assessments were relative to baseline.

A higher score indicates a worsening of global on. In HD, the inclusion of caregiver input is particularly critical for a global assessment as previous studies have demonstrated that patients have limited awareness and recognition of their deficits.

Physical Disability Scale The PDS was used during the study as a measure of disability. Patients were scored on a scale from (“Fixed posture requiring total care - gastrotomy, catheterization”) to 100 al; no disease t”) (Myers 1991).

UHDRS onal Assessments or UHDRS Total Functional Assessment The TFA scale of the UHDRS assessed functionality in 25 tasks of daily living (e.g., “Could patient engage in gainful employment in his/her accustomed work?”). Each question was answered with ‘yes’ or ‘no.

Clinical Global Impression of Severity and Change CGI-S was assessed at baseline and CGI-C is used at all uent time points to assess changes from baseline. The CGI-S scale was initially designed to assess ent response in patients with mental disorders (Guy 1976) but is now used widely in a range of illnesses. Illness severity is rated by the investigator on a 7-point scale (1 = normal, not at all ill to 7 = among the most extremely ill patients). The assessment is based on investigator judgment, ted by a comprehensive, semistructured , patient/caregiver interview. The CGI-C scale measures the change in the patient’s clinical status from a specific point in time, using a 7-point scale, ranging from 1 (very much improved) to 7 (very much worse), with a score of 4 indicating no change.

UHDRS Total Functional Capacity (TFC) The TFC scale of the UHDRS is a standardized scale used to assess 5 functional domains associated with disability shown below (occupation, finances, domestic chores (e.g. y, washing dishes), activities of daily living, and care level). The TFC score has a range of 0-13 and is a well-established endpoint for trials aiming disease progression. The TFC score has been developed and deployed by the Huntington Study Group (HSG, 1996) in multiple trials over 2 decades. The TFC score is accepted by regulators and often considered the most widely accepted tool for disease procession in HD patients. Additionally, TFC is considered the gold standard for ing HD rate of functional decline. Currently, no drug has been shown to slow the decline of TFC despite many attempts. The floor and ceiling s make TFC scores more sensitive to change in early stage HD than in late stage HD.

Functional Capacity:- Occupation: 0 = unable, 1 = al work only, 2 = reduced capacity for usual job, 3 = normal. es: 0 = unable, 1 = major assistance, 2 = slight assistance, 3 = normal.

Domestic Chores: 0 = unable, 1 = impaired, 2 = normal.

Activities of Daily Living (ADL): 0 = total care, 1 = gross tasks only, 2 = minimal im pairment, 3 = Care level: 0 = fill time skill nursing, 1 = home or chronic care, 2 = home.

UHDRS Independence Scale The independence scale of the UHDRS is a rating scale where the patient’s degree of independence was given in percentage, from 10% (tube fed, total bed care) to 100% (no special care needed). Scores must end in 0 or 5 (e.g., 10%, 15%, 20% etc.). Patients with a UHDRS -IS score >90% at the screening visit were not eligible for the study.

Global/Functional Scales Huntington’s e y of Life The HD-QoL is a standardized ment for measuring health-related quality of life. (Hocaoglu 2012). It is a validated disease-specific measure designed for HD, and can provide a summary score of overall health-related quality of life, as well as scores on several discrete . HD-QoL is for people who are living with HD; this includes people who are at risk for HD, people who have tested positive for the huntingtin gene but do not have symptoms, and also for people at early through to late stages of disease. HD-QoL can be used across the full spectrum of HD.

The change from baseline in HD-QoL and in EQ5D-5L was analyzed using an Analysis of Covariance (ANCOVA) Model. The model includes the following fixed effects: treatment, center, neuroleptic use or no use, and baseline HD-QoL or EQ5D-5L score. The last observation carried d (LOCF) was applied for these endpoints for early terminated subjects.

Total Motor Score Subscores UHDRS Hand Movement Score or UHDRS TMS Hand Movement Score The hand movement score is defined as the sum of UHDRS domains finger taps, e-supinate hands and luria (fist-hand-palm test).

UHDRS Gait and e Score or UHDRS TMS Gait and Balance Score The gait and balance score is defined as the sum of UHDRS domains gait, tandem walking and ulsion pull test.

UHDRS Modified Motor Scale or UHDRS TMS Modified Motor Scale The UHDRS-mMS is defined as the sum of following s from UHDRS-TMS: dysarthria, tongue protrusion, finger taps, e-supinate hands, luria, rigidity, bradykinesia, gait, tandem walking, and retropulsion pull test.

UHDRS Chorea or UHDRS TMS Chorea In the UHDRS, maximal chorea was scored from 0 (absent) to 4 (marked/prolonged) on each of the following items: face, mouth, trunk, right upper extremity, left upper extremity, right lower extremity, and left lower extremity. Maximal chorea is the sum of all scores.

UHDRS Dystonia or UHDRS TMS Dystonia In the UHDRS, maximal dystonia was scored from 0 (absent) to 4 (marked/prolonged) on each of the following items: trunk, right upper extremity, left upper extremity, right lower ity, and left lower extremity. Maximal dystonia is the sum of all scores.

TMS Proportion of Responders The percentage of responders, defined as patients with UHDRS-TMS change from baseline ≤0 at Week 26.

Other Motor Assessments Multiple Sclerosis Walking Scale The Multiple Sclerosis Walking Scale (MSWS-12) was adapted to become a generic measure of walking and ty and renamed the Walk-12.

European Quality of Life-3 Dimensions (3 levels) The EQ5D 3 level version (EQ5D-3L) was introduced in 1990 (EuroQol Group 1990). It essentially consists of the EQ5D descriptive system and the EQ visual analogue scale (EQ VAS). The L descriptive system comprises the following 5 dimensions: mobility, self-care, usual activities, iscomfort and anxiety/depression. In developing the 5L, the 5-dimensional structure of the original EQ5D-3L was retained but the levels on each dimension were expanded to 5-levels based on ative and quantitative studies conducted by the EuroQol Group. The labels for each of the dimensions are: no problems, slight problems, moderate problems, severe problems, and unable to/extreme problems. The EQ-VAS is still an integral part of the EQ5D-5L but has been d to make it more user-friendly. The respondent is asked to indicate r health state choosing the most appropriate statement in each of the 5 ions. The EQ VAS records the respondent’s self-rated health on a vertical, visual analogue scale where the endpoints are d ‘Best imaginable health state’ and ‘Worst able health state’. This information can be used as a quantitative measure of health outcome as judged by the individual respondents. It should be noted that the ls 1-3 have no arithmetic properties and should not be used as a cardinal score. The EQ5D can be ted by the patients with caregiver/informant ance if needed.

Quantitative Motor (Q-motor) Assessments Motor deficits can be objectively assessed using different Q-Motor assessments. All Q-Motor assessments are based on the application of precalibrated and temperature controlled force transducers and nsional on sensors with very high sensitivity and test-retest reliability across sessions and sites in a multicenter clinical study. Q-Motor measures thus aim to reduce the limited sensitivity of categorical clinical rating scales, the intra- and inter-rater variability, and placebo effects observed in scales such as UHDRS-TMS. In addition, r assessments allow for the objective monitoring of unintended motor side-effects in clinical studies. Thus, Q-Motor is an objective, reliable, and sensitive measure of motor function that is free of rater bias and limits placebo effect influence.

Figure 36 shows the Q-motor tap measurements for a normal patient, a patient with mild defects and a patient with severe defects. In HD, the largest natural history study of pre-manifest and early stage HD Q-motor tapping deficits correlated with clinical scores as well as regional brain atrophy (Figures 36 and 37 and Bechtel 2010).

Digitomotography (Speeded Index Finger Tapping) The patient placed their hand on a hand rest with their index finger positioned above a forcetransducer.

Recordings start after practice runs. The patient is instructed to finger tap as fast as possible between 2 ry cues. The ing of a tap is defined as a rise of the force by 0.05 N above maximal baseline level. The tap ends when it drops to 0.05 N before the maximal baseline level is reached again. The duration and variability of tap durations (TD), inter onset intervals (IOI), inter peak intervals (IPI), and inter tap intervals (ITI) are the atory e measures for speeded tapping. In addition, variability of peak tapping forces (TF) is calculated as coefficient of variation, and the tapping frequency (Freq), i.e., the number of taps between the onsets of the first and the last tap divided by the time in between, is determined. Five trials of 10 seconds duration are performed with each hand.

Dysdiadochomotography (Pronation/Supination Hand g) This task assesses the rity of hand taps performed when alternating between the palm and dorsal surface of the hand performing a repetitive pronation/supination movement. The force and duration of the hand taps are recorded similarly to the speeded tapping task. A tone cues the start and end of an assessment. Five trials of 10 seconds duration are performed with each hand.

UHDRS Pronation/Supination assessment An assessment of the ability to rotate the forearm and hand such that the palm is down tion) and to rotate the forearm and hand such that the palm is up (supination) on both sides of the body.

Manumotography and Choreomotography (Grip Force and Chorea Analysis) This task assessed the coordination of isometric grip forces in the precision grip between the thumb and index finger. Grip forces are ed during grip tion, object transport, and in a static holding phase. Patients are instructed to grasp and lift a device equipped with a force transducer and nsional position sensor in the precision grip between thumb and index finger and hold it stable adjacent to a marker 10-cm high. Grip forces and 3-dimensional position and orientation of the object are recorded. Mean isometric grip forces and grip force variability in the static phase (expressed as coefficient of variation = standard deviation [SD]/mean × 100) (GFV-C) are calculated during a 15- second period starting 8 seconds after the first cueing tone. Five trials of 20 seconds duration are performed with each hand. Chorea is assessed calculating a “position-index” and “orientation-index”.

Start and end of assessment are signaled by a cueing tone.

Pedomotography (Speeded Foot Tapping) The patient placed a foot on the foot device such that the ball of the foot is positioned above a forcetransducer.

Recordings started after practice runs. The patient is instructed to tap with the foot as fast as possible n 2 auditory cues. The beginning of a tap is defined as a rise of the force by 0.05 N above maximal baseline level. The tap ends when it dropped to 0.05 N before the maximal baseline level is reached again. The duration and variability of TD, IOI, IPI, and ITI are the exploratory outcome measures for speeded tapping. In addition, variability of peak TF is calculated as cient of variation, and the tapping Freq, i.e., the number of taps between the onsets of the first and the last tap divided by the time in n, is determined. Five trials of 10 s duration are med with each foot.

Timed Up and Go Test The TUG is a simple test used to assess a person’s ty and requires both static and dynamic balance. It uses the time that a person takes to rise from a chair, walk 3 meters, turn around, walk back to the chair, and sit down. During the test, the person is expected to wear their regular footwear and use any mobility aids that they would normally require. The TUG is used frequently in the elderly population, as it is easy to administer and can generally be completed by the majority of older adults.

The test is quick, requires no special ent or training, and is easily included as part of the e l examination (Podsiadlo 1991). The use of the TUG test in conjunction with UHDRS has been recommended for clinical studies of HD (Rao 1991).

HD-Cognitive ment Battery (CAB) The CAB may be used to detect symptomatic, ognitive” effects (6 months-1 year) and g rate of cognitive decline (> 1 year). It in 6-12 months after beginning treatment, the CAB is especially useful to measure “pro-cognitive” effects and in more than 1 year the CAB is especially useful to detect the slowing rate of cognitive decline. The CAB covers s most ed in HD, using tests with good psychometric properties. The ing six sections describe the tests that are part of the CAB brief. 1. Symbol Digit Modalities Test The SDMT is a and-pencil test of psychomotor speed and working memory. Participants view a ‘key’ at the top of the page containing symbols paired with s. The remainder of the page displays rows of symbols, and the participant has 90 seconds to write the corresponding number that matches each symbol. 2. Emotion Recognition Emotion recognition of facial expressions of emotions is examined using computerized presentations of photographs depicting 6 basic emotions or a neutral expression. Participants are asked to te the n expressed in each photograph by selecting from the words fear, disgust, happy, sad, surprise, angry, and neutral (10 stimuli per emotion). 3. Trail Making Tests A and B Visual attention and task ing are assessed using the Trail Making test, which consists of 25 circles on a standard sheet of paper. For Trail A, participants are required to connect, as quickly as possible, circles containing numbers in ascending numerical order. For Trail B, participants are to connect, as quickly as possible, circles containing numbers and letters, alternating between numbers and letters in ascending order (e.g., 1, A, 2, B, 3, C, etc.). Trail A is used only as part of the training.

(Bowie 2006). Trail A is used only as part of the training. 4. Hopkins Verbal Learning Test, revised The HVLT-R offers a brief ment of verbal learning and memory (recognition and recall). It is easy to administer and score and is well tolerated even by significantly impaired individuals. Its use has been validated with brain-disordered populations (e.g., Alzheimer's disease, HD, amnestic disorders) as a measure of verbal learning and memory. Each form consists of a list of 12 nouns (targets) with 4 words drawn from each of 3 semantic categories. The semantic categories differ across the 6 forms, but the forms are very similar in their psychometric properties. Raw scores are derived for Total Recall, Delayed Recall, Retention (% retained), and a Recognition Discrimination Index. The HVLT-R has high test-retest ility, and its construct, concurrent, and discriminant validity have been well established. Raw scores are derived for Learning Trials 1-3 (i.e., Total Recall) and Trial 4 (e.g., Delayed Recall Trial).

. Paced Tapping test Psychomotor function is assessed in a Paced Tapping test (also known as PTAP). Participants tap on left and right mouse buttons, alternating between thumbs, at 3.0 Hz. They first listen to a tone presented at the desired g rate, and then begin tapping to the tone. After 11 taps with the tone, the repetition of the tone is discontinued, and participants attempt to continue tapping at the same rate until the end of the trial (31 taps later). 6. One Touch Stockings of Cambridge (OTS) OTS is a spatial planning task which gives a measure of frontal lobe function. OTS is a variant of the Stockings of Cambridge task, and places greater demands on working memory as the participant has to visualize the solution. As with Stockings of Cambridge, the participant was shown 2 displays containing 3 colored balls. The displays are ted in such a way that they can easily be perceived as stacks of colored balls held in stockings or socks suspended from a beam. This arrangement makes the 3 dimensional concepts involved nt to the participant, and fits with the verbal ctions.

There is a row of ed boxes along the bottom of the screen. The test administrator first demonstrates to the participant how to use the balls in the lower display to copy the pattern in the upper display, and completes 1 demonstration m, where the solution es 1 move. The participant must then complete 3 further problems, 1 each of 2 moves, 3 moves, and 4 moves. Next, the participant is shown further problems, and must work out in their head how many moves the solutions to these problems e, then touch the appropriate box at the bottom of the screen to indicate their response.

Problem Behaviors Assessment-Short Form (PBA-s) Because of the prominence of psychiatric symptoms in HD, it was recommended that the PBA-s form be used in all HD studies with any need for behavioral assessment as a comprehensive screen for the most common psychiatric symptoms in HD. (Craufurd 2001, Kingma 2008) The PBA-s also es questions ning suicidal behavior, a particular n in HD. The PBA-s is based on the same set of core behavioral ms as the UHDRS Behavioral questions, which were used previously as the global psychiatric measure in most HD studies. The PBA-s has more detailed questions and more specific guidance on administration and scoring The PBA-s is a brief semi-structured interview covering the most common oral and psychiatric manifestations of HD. The interview is not restricted to a single construct, but rather covers several broad symptom domains relevant to HD, comprising 11 items: low mood, suicidal ideation, anxiety, irritability, anger/aggressive behavior, loss of motivation, erative thinking or behavior, obsessive-compulsive behaviors, paranoid thinking, hallucinations, behavior tive of disorientation. Each symptom is rated for severity on a 5-point scale according to detailed scoring criteria which roughly correspond to the following: 0 = “not at all”; 1 = trivial; 2 = mild; 3 = moderate pting everyday activities) and 4 = severe or intolerable. Each symptom is also scored for frequency on a 5-point scale as follows: 0 = symptom absent; 1 = less than once weekly; 2 = at least once a week; 3 = most days (up to and including some part of everyday); and 4 = all day, every day.

Severity and frequency scores are multiplied to produce an overall ‘PBA score’ for each symptom. ment of Safety In this Example, safety was assessed by qualified study staff by evaluating the following: reported AEs, clinical laboratory test results, vital signs measurements, ECG findings, physical and neurological examination findings (including body ), and itant medication usage.

Clinical Laboratory Tests Clinical tory tests (serum try including electrolytes, hematology and urinalysis) were performed as listed below.

The following serum chemistry tests were med: calcium; phosphorus; sodium; magnesium; potassium; chloride; bicarbonate or carbon dioxide; glucose; blood urea nitrogen; creatinine; cholesterol; uric acid; ALT; AST (aspartate aminotransferase); lactate dehydrogenase; gamma- glutamyl transpeptidase (GGT); alkaline phosphatase; creatine phosphokinase (in case of elevated creatine okinase, the MB fraction should be measured); total n, albumin; total bilirubin; direct bilirubin; indirect bilirubin; and prolactin. The following hematology tests were performed: obin; hematocrit; red blood cell (RBC) count; platelet count; white blood cell (WBC) count and differential count; absolute neutrophil count; absolute lymphocyte count; absolute eosinophil count; absolute monocytes count; te basophil count; and absolute atypical lymphocyte count.

Urinalysis includes testing for the ing: Protein; glucose; ketones; blood (hemoglobin); pH; specific gravity; leukocyte esterase; microscopic; bacteria; RBCs; WBCs; casts; and ls.

Vital Signs Vital signs, including pulse, blood re, and body temperature were measured.

Assessment of Pharmacokinetics and Pharmacogenomics The primary PK measure is a determination of plasma concentration of pridopidine. Concentrations were also incorporated into a pridopidine population PK model and individual exposure for the study patients (Cmax and AUC) was calculated.

Blood Sampling and Handling Blood samples (4 mL each) were collected for the determination of plasma concentrations via venipuncture or indwelling catheter in the g before study drug administration at the following visits: Titration Period: d ay 0 ine) – prior and 1 to 2 hours post first dose and day 14 – 1 to 2 hours post afternoon dose. Full Treatment Dose Period: d ay 28 – pre afternoon dose and 1 to 2 hours post afternoon dose, day 42 – pre afternoon dose and 1 to 2 hours post afternoon dose, day 84 – 1 to 2 hours post afternoon dose, day 112 – pre afternoon dose and 1 to 2 hours post afternoon dose, day 140 – 1 to 2 hours post afternoon dose, day 182 – prior to morning dose, and follow-up visit.

Analysis of Samples Samples were analyzed using an appropriate validated method for idine and its main lite 65 (previously called ACR30). The lower limits of quantification for pridopidine and TV- 45065 in plasma are approximately 1.6 to 1.8 ng/mL and 1.5 to 1.9 ng/mL, respectively.

Pharmacogenomic Variables A blood sample (10 mL) was collected in 2 dipotassium ethylenediaminetetraacetic acid (K2EDTA) plastic tubes at the screening visit for genetic analyses. Analyses include CAG repeats, CYP2D6 status, and genetic long QT syndrome, or any other genetic analyses related to pridopidine response or Primary Efficacy Analysis The change from baseline in UHDRS-TMS was analyzed using a Repeated es model (SAS® MIXED procedure with ED sub-command). The model es the following fixed effects: categorical week in study by treatment interaction, center, neuroleptic use or no use, and baseline UHDRS-TMS score. The unstructured covariance matrix for repeated observations within patients was used. In case that the model does not converge, the Maximum-Likelihood (ML) estimation method is used instead of the default Restricted ML (REML). If the model still does not converge then a simpler covariance structures with less parameters is used, according to the following order: Heterogeneous Autoregressive(1) )], Heterogeneous Compound Symmetry (CSH), gressive(1) [AR(1)], and Compound Symmetry (CS). The estimated means at the Week 26 visit of the change from baseline in UHDRS-TMS was compared between the active treatment arms) and the placebo arm. ivity Analysis A sensitivity analysis to evaluate if the observed effect in UHDRS-TMS is driven by the Chorea UHDRS-TMS sub-score, the Dystonia TMS sub-score, or the Involuntary Movements (Chorea + Dystonia) UHDRS-TMS sub-score was performed as follows: Three variables were calculated: (1) The change from baseline to Week 26 and Week 52 in the sum of the UHDRS-TMS items except the Chorea items, (2) The change from ne to Week 26 and Week 52 in the sum of the UHDRS-TMS items except the Dystonia items, and (3) The change from ne to Week 26 and Week 52 in the sum of the UHDRS-TMS items except the Chorea and ia items. These variables were ed in the same way as the primary efficacy endpoint except that the variable evaluation at baseline were included in the model instead of baseline UHDRS-TMS.

Pharmacokinetic Analysis Plasma concentration data on pridopidine and the main metabolite 65 are presented by descriptive statistics by dose of pridopidine and also by CYP2D6 metabolizer status. Concentrations are also incorporated into a pridopidine population PK model and individual exposure for the study patients (Cmax and AUC) are calculated.

Patient ition by Treatment Group ___________________________Pridopidine___________________________ Analysis group, n (%) Placebo 45mg bid 67.5 mg bid 90 mg bid 112.5 mg bid All Total Screened 492 Screened, not in ITT population 84 Death 0 Adverse event 0 Withdrawal by subject 11 Inclusion criteria not met 20 Exclusion criteria met 46 Lost to follow-up 0 Other 7 ITT population 82 (100) 81 (100) 82 (100) 81 (100) 82 (100) 326 (100) 408 (100) ITT population, not treated 0 0 0 0 0 0 0 Safety population (SP) 82 (100) 81 (100) 82 (100) 81 (100) 82 (100) 326 (100) 408 (100) PK tion (PK) 0 0 0 0 0 0 0 Full analysis set (FAS) 81 (99) 75 (93) 79 (96) 81 (100) 81 (99) 316 (97) 397 (97) Full analysis set on study drug (FASOD) 81 (99) 75 (93) 79 (96) 81 (100) 81 (99) 316 (97) 397 (97) Complete 26 weeks of treatment (CO) 70 (85) 59 (73) 65 (79) 67 (83) 62 (76) 253 (78) 323* (79) ___________________________Pridopidine___________________________ Analysis group, n (%) Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid All Total Discontinued treatment during 1st period 12 (15) 22 (27) 17 (21) 14 (17) 20 (24) 73 (22) 85* (21) Death 0 0 0 0 0 0 0 Adverse event 5 (6) 6 (7) 11 (13) 11 (14) 14 (17) 42 (13) 47 (12) Withdrawal by subject 3 (4) 9 (11) 3 (4) 0 3 (4) 15 (5) 18 (4) Non-compliance 2 (2) 1 (1) 1 (1) 0 0 2 (<1) 4 (<1) Protocol violation 1 (1) 1 (1) 1 (1) 1 (1) 0 3 (<1) 4 (<1) Pregnancy 0 0 0 0 0 0 0 Lost to follow-up 0 0 0 0 0 0 0 Lack of efficacy 0 0 1 (1) 0 0 1 (<1) 1 (<1) Other 1 (1) 5 (6) 0 2 (2) 3 (4) 10 (3) 11 (3) Discontinued treatment during 1st period but 1 (1) 0 2 (2) 2 (2) 2 (2) 6 (2) 7 (2) continue to FU Complete 26 weeks of study 70 (85) 61 (75) 66 (80) 67 (83) 66 (80) 260 (80) 330 (81) Signed ol ent 4 59 (72) 55 (68) 60 (73) 62 (77) 57 (70) 234 (72) 293 (72) Entered 2nd period 57 (70) 49 (60) 54 (66) 56 (69) 46 (56) 205 (63) 262 (64) Started treatment for 2nd period 57 (70) 49 (60) 52 (63) 56 (69) 46 (56) 203 (62) 260 (64) ITT population for the 52 Weeks Analysis (ITT2) 82 (100) 81 (100) 82 (100) 81 (100) 82 (100) 326 (100) 408 (100) Safety population for the 52 Weeks Analysis (SP2) 82 (100) 81 (100) 82 (100) 81 (100) 82 (100) 326 (100) 408 (100) PK population for the 52 Weeks Analysis (PK2) 0 0 0 0 0 0 0 ___________________________Pridopidine___________________________ Analysis group, n (%) Placebo 45 mg bid 67.5 mg bid 90 mg bid 112.5 mg bid All Total Full is set for the 52 Weeks Analysis (FAS2) 81 (99) 75 (93) 79 (96) 81 (100) 81 (99) 316 (97) 397 (97) Complete 52 weeks of treatment 52 (63) 43 (53) 44 (54) 53 (65) 44 (54) 184 (56) 236 (58) Discontinued treatment during 2nd period 5 (6) 6 (7) 8 (10) 3 (4) 2 (2) 19 (6) 24 (6) Death 0 0 0 1 (1) 0 1 (<1) 1 (<1) e event 1 (1) 4 (5) 5 (6) 0 1 (1) 10 (3) 11 (3) Withdrawal by subject 2 (2) 1 (1) 2 (2) 0 0 3 (<1) 5 (1) Non-compliance 1 (1) 0 0 0 0 0 1 (<1) Protocol violation 0 0 0 0 0 0 0 Pregnancy 0 0 0 0 0 0 0 Lost to follow-up 0 0 0 0 0 0 0 Lack of efficacy 1 (1) 0 0 1 (1) 1 (1) 2 (<1) 3 (<1) Other 0 1 (1) 1 (1) 1 (1) 0 3 (<1) 3 (<1) Discontinued treatment during 2nd period but 0 1 (1) 2 (2) 0 1 (1) 4 (1) 4 (<1) continue to FU Complete 52 weeks of study 52 (63) 43 (53) 46 (56) 52 (64) 44 (54) 185 (57) 237 (58) Stages of Huntington's Disease Many clinicians and diagnosticians adopt the Shoulson and Fahn rating scale, based on TFC scores, to follow progression of HD. This rating scale groups total TFC scores into five stages of disease, with lower stages indicating more intact functioning. Table 4, below, provides the TFC scores, average years from diagnosis and broad guidelines for typical care level for each stage of disease. (Johnson 2014.) Table 5 below shows the number of patients at each TFC stage participating the study.

Table 4.

Stage TFC Years since motor l abilities and care level score diagnosis 1 11-13 0-8 Able to work at least part time, may require slight assistance in one of finances, domestic chores or ADL basic functions 2 7-10 3-13 Unable to work, requires some assistance in some basic functions 3 3-6 5-16 Unable to work, requires major assistance in most basic functions 4 1-2 9-21 Requires major ance in all basic functions and although comprehension may be intact requires ance to act. 0 11-26 Requires major assistance in all basic functions and full time g care Patients with stage 1 or 2 have the steepest rate of natural decline and are the most ive to the clinical measure described in this application. TFC and HD-CAB assessments are designed specifically for patients with stage 1 or 2 and earlier. Patients with stage 3, 4 or 5 often have difficult completing assessments, the floor and g limit the ability to track change and have very significant brain tissue loss.

Early stage HD, as used herein, means stage 1 or stage 2 HD (BL TFC > 7) as defined by Table 4 above.

Table 5. HD stages of patients in study N % years since HD diagnosis Stage 1 (TFC= 11-13) 78 19 3 Stage 2 (TFC= 7-10) 218 53 5 Stage 3 (TFC= 3-6) 101 25 5-8 Stage 4 (TFC= 1-2) 10 2 >8 Stage 5 (TFC= 0) 1 <1 Results The results of this example are shown in Figures 1-47 and Table 6 below.

Table 6.

Responder is Questions Observed Data Analysis 45mg bid Placebo N=37 N=41 What tion of early stage subjects had no 30 (81%) 20 (49%) deterioration on TFC (score ≥0) at 52 weeks? p value (Chi-Square) 0.003 What proportion of early stage subjects had an 10 (27%) 5 (12%) improvement of ≥1 points on TFC at 52 weeks? p value (Chi-Square) 0.099 A significant difference in the proportion of subjects that showed no decline in TFC over 52 weeks was observed between patients treated with 45 mg bid and patients receiving placebo.

Overview of preliminary analysis of functional, atory endpoints and safety: Endpoints not dependent on rater bias were less prone to placebo effect, such as the Q-motor assessment. The signals detected suggest biological effects of pridopidine. Total Functional Capacity (TFC) showed trends favoring idine after 26 weeks of treatment. There was no major safety findings despite high doses.

Preliminary results on TFC scores - Considerations Expected deterioration of about 0.5 points were seen in the placebo group at 6 months. Historical data indicates that TFC deteriorates about 1 point per year in patients with HD. TFC starts showing separation from placebo at week 12 to 20 and separation becomes a strong trend at week 26. The TFC data supports a finding that idine causes a delay of progression of functional decline.

Figure 42 shows a graphical representation of TFC deterioration at the different stages of e.

Without wishing to be bound to this theory, the treatment effects shown in the figures were more pronounced when treating early stage patients (including stages 1 and 2), especially early stages with baseline TFC greater than or equal to 7, and even more so in stage 1 (BL TFC =11-13). t wishing to be bound to this theory this is particularly true for TFC es and ADL, TFC domestic chores, dystonia, involuntary movements (dystonia and chorea), gait and balances. A patient affected with HD with a baseline TFC score of 11-13 is considered to be a stage 1 HD patient.

Potential placebo effect contributors in this Example The ing items may account for the placebo effect seen in this e: Rater bias, a lack of hope in HD, together with a high expectation for an ive treatment and a desire to get better from patients, overall positive data with idine ent causes high expectations, patients have an 80% chance to receive active treatment, a high number of pills may cause expectancy, protocol changes during the study, and the number of assessments per visit.

Dystonia The total Dystonia treatment exemplified in this application is representative of treatment of the following types of ia: early onset generalized dystonia (DYT1 and non-DYT1), Focal ia, Musicians' dystonias, Dopa-responsive dystonia, Myoclonus dystonia, Paroxysmal dystonias and dyskinesias, X-linked dystonia-parkinsonism, Rapid-onset ia-parkinsonism, Secondary dystonias, dystonia in HD patients and Psychogenic dystonia. In particular, the present invention relates to treating dystonia in a HD patient, for example an early stage (stage 1 or stage 2) HD patient.

Discussion Pridopidine efficacy Pridopidine has previously demonstrated motor function benefit in 2 large, -blind, placebocontrolled studies in patients with HD (HART and MermaiHD). The primary endpoint for both studies was the mMS. Both studies provided evidence of a beneficial effect on the UHDRS–Total Motor Score (TMS), demonstrating differences favoring idine 45 mg twice daily (bid) compared with placebo. In a pooled analysis of the 2 studies, pridopidine 45 mg bid significantly improved TMS compared with placebo at weeks 12 and 26 (Landwehrmeyer 2011). The PRIDE-HD study used change in TMS from baseline to week 26 as the y endpoint to further evaluate the effects of pridopidine at doses ranging from 45 mg to 112.5 mg bid.

The PRIDE-HD study recruited patients in all disease stages (i.e. early and ed), and 72% of the patient population treated with pridopidine were in the early stages of the disease (Stage 1 and 2 HD; baseline TFC scores of 7 to 13 (HD1 and HD2)). During the early stages of HD many of the HD clinical scales and ment tools are the most sensitive to change over time. PRIDE-HD did not meet the primary endpoint compared with placebo due to a large placebo effect. However, analysis of data from the PRIDE-HD study demonstrated less decline in the UHDRS-TFC score in ts receiving pridopidine 45 mg bid compared with those receiving placebo (no correction for type I error for multiple comparisons was applied). This effect was most evident in patients with early-stage HD.

Figures 1 and 2 are graphs showing pridopidine tration ) measures in patients’ blood h week 20 of treatment.

Figures 3-5 are graphs showing change in UHDRS TMS over time. A lower number represents improvement. Figure 3 shows a comparison between doses in the PRIDE-HD study. Figure 4 shows the placebo effect in the UHDRS TMS, which was greater in the PRIDE-HD study than in the MermaiHD or HART studies. Figure 5a shows an improvement in UHDRS TMS for both 45mg pridopidine bid and 90mg pridopidine bid in the PRIDE-HD study compared to the placebo in MermaiHD and HART studies. Figure 5b shows an overall improvement in UHDRS-TMS for 45mg pridopidine bid over 52 weeks.

UHDRS Total Functional ty (UHDRS TFC) The data in this application demonstrates that pridopidine shows an effect on progression of HD as measured by total functional capacity (TFC). This effect on TFC was statistically significant in the full analysis set and even more pronounced in early stage HD patients. Early stage HD ts are d as those with a baseline (BL) TFC score of greater than or equal to 7 (Stage1 and Stage 2).

There was a significant lessening in UHDRS TFC between patients administered pridopidine at some doses compared to patients administered the placebo at 52 weeks in both the Full Analysis Set (FAS) and the early stage HD sub-population. Patients with early stage HD (baseline 7-13) receiving idine have more positive TFC results than patients with late stage HD (baseline 0-6) receiving pridopidine. The effect on TFC observed at 26 weeks reached significance in the early stage subpopulation (Figures 10b and 20b). The effect on TFC observed at 52 weeks reached significance in the early stage subpopulation (Figures 10d, 11d, 21j, 31b).

The TFC annual decline of the placebo group shown in, for example, Figures 10c, 21j, and 31a, was comparable to the TFC annual decline reported in the literature and observed in historical placebo arms. As shown by, for example, Figures 10b, 10d, 21b, 21j, 21n, and 31b , the TFC deterioration in patients given placebo was higher in ts with early stage HD. This data shows a slowing of clinical progression in HD as measured by TFC and is the first clinical trial to do so among eleven (11) other al trials. Significance was observed in the UHDRS TFC at week 26 (figure 21b) TFC finance at week 26 (figures 12b, 21d, 21f), TFC finance and ADL at week 26 (Figure 11b, 21d), TFC ADL at week 26 (Figure 21h) and UHDRS TFC at week 52 (figure 21j) TFC e at week 52 (figures 21n), TFC finance and ADL at week 52 (Figure 11e, 21l), TFC ADL at week 52 (Figure 21p).

UHDRS Independence Scale (UHDRS IS) The UHDRS-IS comprises part of the UHDRS functional assessments (Huntington Study Group 1996). It is a rating scale where the patient’s degree of independence is given in percentage, from 10% (tube fed, total bed care) to 100% (no special care needed). Scores must end in 0 or 5 (eg, 10%, 15%, % etc). The scale was assessed at screening, baseline, weeks 4, 12, 20, 26/Early Termination, 28 and 52/Early ation.

The change from baseline in the UHDRS-IS week 52 is shown in Figure 9e. The change from baseline in the UHDRS-IS assessed at week 52 decreased across treatment groups, but was not statistically significant in any treatment group. For the placebo group, there was a decrease (indicating a trend toward e) in IS at Week 52. Positive trends in the desired direction were observed in early-stage HD ts ine TFC score ≥7) at week 52 (see Figure 20d). No clinically meaningful changes were noted for patients with a baseline TFC<7. The Independence scale supports the TFC effect, which provides a gence of endpoints.

UHDRS TMS and Motor Endpoints Motor effects were statistically significant in stage 1 subpopulations. For example, statistically significant changes are seen in the HD Stage 1 patient subgroups for Total TMS, Involuntary movements (Dystonia, Chorea), Ambulation (TMS Gait and Balance, Time Up an Go, Walk 12). The improvement in ambulation may be contributing to TFC data.

A large placebo response masked motor effects in the full analysis set. However, in early HD there was a tically significant effect on TMS at weeks 26 (Figure 8b) and 52 (Figure 8d) driven by a lower placebo effect. Involuntary Movements (chorea and ia) as measured by TMS improved in HD stage 1 patients at 26 weeks (Figure 8n). The effect persisted at 52 weeks as well (Figure 8p).

Effects were ed primarily with 45mg bid and 90mg bid, suggesting a non-linear dose response.

In addition, positive effects on ambulation (such as gait, timed up and go, and stair climbing) were observed in early stage patients administered 45 mg pridopidine bid (see for example Figures 18b, 18d, 19b, 19d).

PBA-s The PBA-s is a brief semi-structured interview covering the most common behavioral and psychiatric manifestations of HD. The interview is not restricted to a single construct, but rather covers several broad m domains relevant to HD, comprising 11 items: low mood (depression), suicidal ideation, anxiety, irritability, anger/aggressive behavior, loss of motivation (apathy), perseverative thinking or behavior, obsessive-compulsive behaviors, id ng, hallucinations, and behavior suggestive of disorientation. Each symptom is rated for severity on a 5-point scale according to detailed scoring criteria, which roughly correspond to the following: 0 = “not at all”; 1 = trivial; 2 = mild; 3 = moderate (disrupting ay activities) and 4 = severe or intolerable. Each symptom is also scored for frequency on a 5-point scale as s: 0 = m absent; 1 = less than once weekly; 2 = at least once a week; 3 = most days (up to and including some part of every day); and 4 = all day, every day.

Severity and ncy scores are multiplied (after setting all values outside the range of 0-4 to missing) to produce an overall "PBA-s score" for each symptom. The total PBA score is calculated by the sum of all PBA-s scores across symptoms/domains.

The PBA-s assessments were ted at baseline, weeks 4, 12, 26, and 52.

The change from baseline to week 26 in the PBA-s domains and total scores did not show meaningful s es 17a-d, 17i ). However, the change from baseline to week 52 in the PBA-s total score as well as several of the PBA-s domains showed a trend to improvement or icant improvement (Figures 17d-17h) . In the full analysis set, the pridopidine 45 mg bid group showed a trend t oward improvement in the PBA-s total score at 52 weeks compared with the placebo group (∆3.98 points to placebo, p=0.0603, n=75) (see Figures 17e-17f)). Figures 17j and 17l show a trend to improvement in PBA apathy in early stage patients at 26 weeks and 52 weeks, respectively. Figure 17r shows a significant improvement in PBA disorientation in early stage patients at 26 weeks for 45mg bid., respectively. nitive Assessment Battery The PRIDE-HD study was the first large study to include the nitive Assessment Battery (HDCAB ) assessments (Stout et al 2014). The HD-CAB was designed to detect symptomatic, ”pro- cognitive” effects (6 months-1 year) and slowing rate of cognitive decline (> 1 year) in late ifest , HD1 and HD2 patients. It covers ive domains most impacted in HD, using tests with good psychometric properties. The battery includes the following tests: Symbol Digit Modalities Test, Emotion Recognition, Trail Making Test B, Hopkins Verbal Learning Test (revised), Paced Tapping at 3 Hz, and One Touch Stockings of Cambridge.

For the 6 domains of the HD-CAB, there was no consistent pattern of improvement or e as demonstrated by the mean changes from baseline for the pridopidine or placebo treatment .

Positive findings indicating potential improvement from baseline in the Paced Tapping at 3 Hz ment (a measure of psychomotor on) were observed in the full analysis set at week 52 for the 45 mg bid treatment group (see Figure 41d).

Example 2: Effect of Pridopidine on Functional Capacity of Patients with Huntington Disease Objective To explore functional decline measured by the Total Functional Capacity (TFC) scale in patients treated with open-label pridopidine 90 mg/day for 36 months HART) and compare s to historical cohorts of placebo patients enrolled in HSG-sponsored trials (CARE-HD and 2CARE).

Background Patients with HD experience motor, cognitive and behavioral symptoms that lead to s, long- term disability. TFC (range 0–13, high scores indicate greater capacity) evaluates patients’ capacity to work, handle es and ic chores, perform activities of daily living and live independently, and is most sensitive to early changes in lity. TFC was utilized in OPEN-HART and the Coenzyme Q10 studies, CARE-HD and 2CARE.

Methods This is compared the OPEN-HART cohort (n=50) that received pridopidine 90 mg/day and the placebo arms of CARE-HD (n=80) and 2CARE (n=213) without matching on baseline characteristics.

For this analysis, TFC scores at baseline, 12, 24, and 36 months from OPEN-HART and 2CARE, and TFC scores at baseline, 12, 25, and 30 months from CARE were utilized.

Results At baseline, the OPEN-HART cohort had the lowest absolute mean (SD) TFC score compared with the CARE-HD and 2CARE cohorts [9.14(2.78), 10.3(1.7) and 11.05(1.47), respectively].

The mean change from baseline in TFC at 12 months was OPEN-HART: -0.49 (1.60), CARE: -1.00 (1.48) and 2CARE: -1.11 (1.62); at 24 months (OPEN-HART and 2CARE) and 25 months (CARE) was: -1.00 (1.92), -1.80 (2.06) and -2.24 (1.91), respectively; at 36 months HART and 2CARE) was: -1.68 (2.22) and -2.54 (2.53), respectively; and at 30 months (CARE) was: -2.80(2.27).

The results show that the TFC decline over time was slower in patients who received pridopidine in OPEN-HART compared to those who received placebo in CARE-HD and 2CARE. A slowdown in TFC decline was ed, which suggests that pridopidine has neuroprotective and/or diseasemodifying properties.

Example 3: Phase 3 Study The proposed Phase 3 study is a 78-week, multicenter, ized, double-blind, placebo controlled, parallel group study to te the efficacy and safety of pridopidine administered at a dose of 45 mg bid in adult patients with early stage HD.

The study consists of a screening period (up to 8 weeks); a 2-week titration period; a 76-week, double-blind, ose treatment period; and a follow-up period (consisting of an end of study visit at 3 to 4 weeks after the end of treatment .

During the screening period, ts provide ed consent and subsequently undergo assessments to ine eligibility for participation in the study. The stage of HD is established by the UHDRS TFC scale. The TMS and UHDRS-IS is assessed. The TMS assessment is rated by trained raters at the site and also videotaped for central rating by an independent blinded third party (Independent Adjudication Committee (IAC)). An IAC minimize s rater bias and error during screening by reviewing all information ted at screening, including patient medical history, prior to approving any t for ization. le patients are invited to return for a baseline visit and baseline assessments. Those patients who remain eligible for study participation will be randomly assigned (1:1 ratio) to 1 of the 2 treatment groups: 45 mg bid pridopidine or placebo bid. For patients assigned to receive pridopidine, the dose is titrated during the first 2 weeks from 45 mg qd to the final dose of 45 mg bid pridopidine.

During titration (days 0 to 14), patients receive 1 scheduled telephone call (TC) during the second week. Patients attend on-site clinic visits at weeks 26, 52, and 78 for safety and efficacy measures and blood sampling for pharmacokinetic assessments. At weeks 3, 6, 12, 39 and 65, safety visits will be conducted either by a visiting nurse at home or at the clinic for safety assessments. Patients will receive 1 scheduled TC approximately 6 to 7 weeks following each at home or on-site clinic visit.

During these TCs, patients are asked about the following: adverse events, itant medications, alcohol/drug use, tolerability of study drug, use of benzodiazepines and antidepressants, and compliance. The C-SSRS (since last visit version) and abbreviated PBA-s (a subset of PBA-s questions on depressed mood, suicidal ideation, anxiety, irritability, loss of motivation, and obsessive- compulsive behaviors) are ed.

Patients who complete all scheduled visits have final procedures and assessments performed at the end of treatment visit (week 78). Patients who aw from the study before completing the evaluation period will have the week 78 procedures and assessments performed at their final visit, which is considered their early termination visit.

There is an on-site end of study visit approximately 3 to 4 weeks after the last dose of study drug to evaluate efficacy, safety (including a single ECG), pharmacokinetics, rebound, and dependence.

Primary endpoint: The primary efficacy endpoint to be evaluated is the change from baseline in TFC at week 78 in patients treated with pridopidine 45 mg bid compared to patients receiving placebo. The primary cy analysis is carried out using a linear mixed model for repeated measures with change from ne in the primary nt (TFC) as the dependent variable in the modified intent-to-treat population (randomized patients with at least 1 post-baseline TFC assessment). The model includes visit (4 levels: weeks 12, 26, 52, and 78), treatment group, visit by treatment group interaction, country, HD stage (HD1 or HD2), and neuroleptic use (yes or no) as fixed factors, and includes the corresponding baseline score as a ate. The unstructured covariance matrix for repeated observations within ts is used and the Kenward-Rodger method is used to calculate the denominator degrees of freedom. The primary is for TFC will e the change from baseline to week 78 between the 45 mg bid pridopidine and placebo groups. Lower scores indicate more severe functional impairment than higher scores.

Secondary endpoints: Two secondary endpoints are selected based on the evidence-based trends observed in PRIDE-HD. 1. Change from baseline to week 78 in UHDRS TMS in patients receiving pridopidine 45 mg bid ed with patients receiving placebo.

The TMS is the standard and well-accepted clinical tool for tracking the progression of motor symptoms in patients with HD (Huntington Study Group 1996). The motor section of the UHDRS assesses motor features of HD with standardized ratings of oculomotor function, dysarthria, chorea, dystonia, gait, and postural stability. The TMS is the sum of 31 individual motor ratings, with each ment rated on a 5 point scale from 0 (normal) to 4 ally abnormal). Higher scores indicate more severe motor impairment than lower scores.

Results from the HART and MermaiHD studies suggested a potential benefit for pridopidine in improving motor symptoms in HD (de Yebenes 2011; Huntington Study Group HART Investigators 2013). In the PRIDE-HD study, TMS showed improvement at all doses at week 26, but did not reach statistical icance, likely due to the high and sustained placebo effect, thus ing the ability to assess the potential motor on benefit of pridopidine. The current proposed study incorporates several measures to minimize the placebo effect and to allow an accurate ment of the potential for pridopidine to provide a motor function benefit. 2. Change from baseline to week 78 in the Apathy tion Scale (AES) in patients ing idine 45 mg bid, compared with patients receiving placebo. Apathy is one of the most prevalent neurobehavioral symptoms in HD, ing in approximately 50-70% of the symptomatic HD population, and increases as the disease sses. Symptoms include lack of interest and motivation, inability to start activities, social awal, and emotional flatness. Apathy scores in patients with HD are highly correlated with duration of illness, suggesting that apathy is an inevitable consequence of advanced disease. Although less distressing than symptoms like depression and less disruptive than irritability or aggression, apathy has a considerable adverse impact on those affected with HD because it leads to a decrease of the goal-directed behaviors that contribute much to the day-to-day quality of life (Krishnamoorthy 2011; Martinez-Horta l 2016).

Exploratory analysis in the PRIDE-HD study revealed that Problems Behavioral Assessment apathy sub-score was improved in early HD patients receiving 45 mg bid pridopidine compared with placebo at week 52. An improvement in apathy will provide convergent evidence for clinical utility with the primary endpoint, TFC. The AES was developed to measure abnormalities in goal-directed behavior, goal related thought content, and emotional indifference (Marin et al 1991). This more comprehensive scale was selected as a secondary endpoint, while the PBA will remain as an exploratory endpoint.

The Bonferroni-Holms method to control type 1 error will be used in the following fashion: If the primary nt is achieved, both ary nts will be tested simultaneously at α=0.025. If one of these secondary endpoints is achieved, the other can subsequently be tested at α=0.05.

Throughout this specification and the claims that follow, unless the context requires otherwise, the word ise", and ions such as "comprises" and "comprising", will be understood to imply the ion of a stated integer or step or group of integers or steps but not the exclusion of any other integer or step or group of integers or steps.

The reference in this specification to any prior publication (or information derived from it), or to any matter which is known, is not, and should not be taken as an acknowledgment or admission or any form of suggestion that that prior publication (or ation derived from it) or known matter forms part of the common general dge in the field of endeavour to which this specification relates.

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Claims

1. Use of pridopidine or a pharmaceutically able salt thereof in preparation of a ment for improving or maintaining motor ability, ing or maintaining mobility or reducing or ining involuntary movements in a human subject, wherein the human 5 subject is afflicted with early stage Huntington’s disease.

2. The use of claim 1, wherein motor ability is measured by the UHDRS Total Motor Score (TMS) score.

3. The use of claim 1 or claim 2, a dose of n 90-225 mg of pridopidine is to be administered to the human subject per day. 10

4. The use of any one of claims 1-3, wherein a dose of 90mg, 135mg, or 180mg of pridopidine is to be administered to the human subject per day.

5. The use of any one of claims 1-4, wherein a unit dose of a pharmaceutical composition comprising pridopidine or salt thereof contains 45 mg, 67.5 mg, or 90 mg, of pridopidine.

6. The use of any one of claims 1-5, wherein pridopidine is to be administered twice per day. 15

7. The use of any one of claims 1-6, wherein the pharmaceutical composition is to be administered for more than 26 weeks, at least 52 weeks, or at least 78 weeks.

8. The use of any one of claims 1-7, wherein the human subject has greater than or equal to 36 CAG repeats in the huntingtin gene.

9. The use of any one of claims 1-8, wherein the human subject has a baseline TFC score which 20 is greater than or equal to 9.

10. The use of any one of claims 1-9, wherein the human subject has a baseline TFC score which is greater than or equal to 7.

11. The use of any one of claims 1-10, wherein the human subject has a baseline TFC score of 11-13. 25

12. The use of any one of claims 1-11, wherein the human subject has a ne TFC score of 7-

13. The use of any one of claims 1-11, wherein the pridopidine salt is selected from the hydrochloride, the hydrobromide, the L-tartrate, the nitrate, the perchlorate, the phosphate, the sulphate, the e, the acetate, the ascorbate, the benzenesulphonate, the benzoate, the cinnamate, the citrate, the embonate, the enanthate, the fumarate, the glutamate, the glycolate, the lactate, the maleate, the malonate, the mandelate, the methanesulphonate, the naphthalene- 2-sulphonate, the ate, the salicylate, the sorbate, the stearate, the succinate, the tartrate 5 or the toluene-p-sulphonate salt.

14. The use of claim 13, n the pridopidine is pridopidine hydrochloride. ~$~Hacebo 2000 -------- 45mgbm ww6?5rngbw W90 mg bk} £500 \\\\\\