NZ761237B2 - Mutated protoporphyrinogen IX oxidase (PPX) genes - Google Patents

Mutated protoporphyrinogen IX oxidase (PPX) genes Download PDF

Info

Publication number
NZ761237B2
NZ761237B2 NZ761237A NZ76123711A NZ761237B2 NZ 761237 B2 NZ761237 B2 NZ 761237B2 NZ 761237 A NZ761237 A NZ 761237A NZ 76123711 A NZ76123711 A NZ 76123711A NZ 761237 B2 NZ761237 B2 NZ 761237B2
Authority
NZ
New Zealand
Prior art keywords
plant cell
ppx
plant
gene
cell
Prior art date
Application number
NZ761237A
Other versions
NZ761237A (en
Inventor
Peter R Beetham
Schopke Aura De
Sarah Dumm
Gregory Fw Gocal
James Pearce
Christian Schopke
Keith A Walker
Original Assignee
Cibus Europe Bv
Cibus Llc
Filing date
Publication date
Application filed by Cibus Europe Bv, Cibus Llc filed Critical Cibus Europe Bv
Priority to NZ811395A priority Critical patent/NZ811395A/en
Priority claimed from NZ739553A external-priority patent/NZ739553A/en
Publication of NZ761237A publication Critical patent/NZ761237A/en
Publication of NZ761237B2 publication Critical patent/NZ761237B2/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/001—Oxidoreductases (1.) acting on the CH-CH group of donors (1.3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y103/00—Oxidoreductases acting on the CH-CH group of donors (1.3)
    • C12Y103/03—Oxidoreductases acting on the CH-CH group of donors (1.3) with oxygen as acceptor (1.3.3)
    • C12Y103/03004—Protoporphyrinogen oxidase (1.3.3.4)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants

Abstract

Provided are compositions and methods relating to gene and/or protein mutations in transgenic or non-transgenic plants. In certain embodiments, the disclosure relates to mutations in the protoporphyrinogen IX (PPX) gene, specifically a tyrosine to phenylalanine substitution at position 426 and a phenylalanine to tyrosine substitution at position 145. In some embodiments the disclosure relates to plants that are herbicide resistant.

Claims (9)

1. A plant cell comprising a mutated protoporphyrinogen IX oxidase (PPX) gene, wherein said gene encodes a protein comprising a tyrosine to phenylalanine substitution at the amino acid position corresponding to position 426 of SEQ ID NO: 1 and a phenylalanine to tyrosine substitution at the amino acid position corresponding to position 145 of SEQ ID NO: 1.
2. The plant cell of claim 1, wherein the plant cell is a non-transgenic plant cell.
3. A method for producing a non-transgenic plant cell with a mutated PPX gene, comprising introducing into a plant cell a gene repair oligonucleobase (GRON) with a targeted mutation in a protoporphyrinogen IX oxidase (PPX) gene to produce a plant cell with a PPX gene that expresses a PPX protein comprising a tyrosine to phenylalanine substitution at the amino acid position corresponding to position 426 of SEQ ID NO: 1 and a phenylalanine to tyrosine substitution at the amino acid position corresponding to position 145 of SEQ ID NO: 1.
4. The method of claim 3, for producing a plant resistant to one or more PPX-inhibiting herbicides, further comprising identifying a plant cell having a growth rate or rate of cell division in the presence of a herbicide that is at least 35% of the growth rate or rate of cell division in a corresponding plant expressing the wild-type PPX protein in the absence of the herbicide; and regenerating a non-transgenic plant resistant to one or more PPX-inhibiting herbicides, the plant having a mutated PPX gene from said plant cell.
5. The method of claim 3 or claim 4, wherein said plant cell is of a plant selected from the group consisting of potato, sunflower, sugar beet, maize, cotton, soybean, wheat, rye, oats, rice, canola, fruits, vegetables, tobacco, barley, sorghum, tomato, mango, peach, apple, pear, strawberry, banana, melon, carrot, lettuce, onion, soya spp, sugar cane, pea, field beans, poplar, grape, citrus, alfalfa, rye, oats, turf and forage grasses, flax, oilseed rape, cucumber, morning glory, balsam, pepper, eggplant, marigold, lotus, cabbage, daisy, carnation, petunia, tulip, iris, lily, and nut-producing plants, in particular wherein said plant cell is a species selected from the group consisting of Solanum tuberosum, Oryza sativa, Sorghum bicolor, Ricinus communis, Brassica napus, Glycine max, and Zea mays, or wherein said plant cell is a Russet Burbank potato cultivar.
6. The method of any one of claims 3-5, wherein said plant cell is a canola cell.
7. The method of any one of claims 3-6, wherein said plant is resistant to one or more PPX-inhibiting herbicides selected from acifluorfen-Na, Bifenox, Chlomethoxyfen, fluoroglycofen-ethyl, Fomesafen, Halosafen, Lactofen, Oxyfluorfen, Fluazolate, pyraflufen-ethyl, cinidon-ethyl, Flumioxazin, flumiclorac-pentyl, fluthiacet-methyl, Thidiazimin, Oxadiazon, Oxadiargyl, Azafenidin, carfentrazone-ethyl, Sulfentrazone, Pentoxazone, Benzfendizone, Butafenacil, Saflufenacil, Pyrazogyl, Profluazol, in particular wherein said plant is resistant to one or more herbicides selected from the group consisting of flumioxazin, sulfentrazone, and saflufenacil.
8. A plant cell produced by the method of any one of claims 3-7.
9. The plant cell of claim 1 or claim 2, or the method of any one of claims 3-7, substantially as described herein.
NZ761237A 2010-08-03 2011-08-02 Mutated protoporphyrinogen IX oxidase (PPX) genes NZ761237B2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
NZ811395A NZ811395A (en) 2010-08-03 2011-08-02 Mutated protoporphyrinogen ix oxidase (ppx) genes

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US37043610P 2010-08-03 2010-08-03
NZ739553A NZ739553A (en) 2010-08-03 2011-08-02 Mutated protoporphyrinogen ix oxidase (ppx) genes

Publications (2)

Publication Number Publication Date
NZ761237A NZ761237A (en) 2024-08-30
NZ761237B2 true NZ761237B2 (en) 2024-12-03

Family

ID=

Similar Documents

Publication Publication Date Title
IL294685A (en) Mutated protoporphyrinogen ix oxidase (ppx) genes
JP2013545434A5 (en)
CA2662511C (en) Method for improving plant health by providing dicamba to a dicamba monooxygenase (dmo) transgenic plant or by providing a product of dmo-mediated metabolism to a plant
US8207092B2 (en) Methods and compositions for improving plant health
RU2583658C2 (en) Control dicotyledonous volunteers aad in monocotyledonous crops
RU2017133853A (en) NEW CLASS OF GLYPHOSATE RESISTANCE GENES
WO2012115968A2 (en) Methods of weed control involving aad-1 plants, and re-plant and/or pre-emergence herbicide applications
NZ761237B2 (en) Mutated protoporphyrinogen IX oxidase (PPX) genes
Barupal et al. CRISPR/Cas systems for enhancing photosynthesis: Climate resilience and food production
Laguerre et al. Evaluation of weed control efficacy and crop safety of the PPO-inhibiting herbicide tiafenacil in California orchard cropping systems
NZ739553A (en) Mutated protoporphyrinogen ix oxidase (ppx) genes
AU2022275035B2 (en) Use of protoporphyrinogen oxidase
EP4516911A1 (en) Use of protoporphyrinogen oxidase
Ford et al. The roles of biotechnology in agriculture to sustain food security under climate change
Thada Improvement of heat stress tolerance in maize and sorghum by lipid alterations of the plastidic membrane
WO2025072809A2 (en) Herbicide resistant hppd variants identified via directed evolution
WO2026027888A2 (en) Methods of altering plant stress response
Walsh Selection of Camelina mutants resistant to acetolactate sythase inhibitor herbicides
Andersen et al. Linkage disequlilibrium and associations with forage quality at loci involved in monolignol biosyntheis in breeding lines of European silage maize (Zea mays L.)
Studer et al. Development and high-density mapping of gene-derived SSR markers in perennial ryegrass (Lolium perenne): Poster 3
Ulvskov Pectins as fine chemicals in food and medicine
Asp et al. The DAFGRI genome project-completed
Asp et al. Identification and characterization of T-DNA mutants for the laccase gene family in Arabidopsis: Poster 13
Uzarowska et al. Functional analysis of genes involved in heterosis for plant height in maize by expression profiling: Poster 2
Ingvardsen et al. Molecular analysis of sugarcane mosaic virus (SCMV) resistance in maize: Poster 4