NZ755760B2 - Method for non-invasive monitoring of fluorescent tracer agent with background separation corrections - Google Patents

Abstract

method of monitoring a time-varying fluorescence emitted from a fluorescent agent from within a diffuse reflecting medium with time-varying optical properties is disclosed that includes providing at least two measurements obtained from a patient before and after administration of the fluorescent agent that includes anFlrsignal detected adjacent to the medium by a filtered light detector during illumination of the medium by excitatory-wavelength light, and at least one DR signal selected from: a and signal. The method further includes identifying a post-equilibration portion of the measurement data set and transforming each DRsignal within the post-equilibration portion of the measurement data set to an signal representing a detected fluorescence intensity emitted solely by the fluorescent agent from within the medium. The disclosed method includes removing the effects of leak-through of excitation-level light and removing the effects of autofluorescence from the DRsignal. gent that includes anFlrsignal detected adjacent to the medium by a filtered light detector during illumination of the medium by excitatory-wavelength light, and at least one DR signal selected from: a and signal. The method further includes identifying a post-equilibration portion of the measurement data set and transforming each DRsignal within the post-equilibration portion of the measurement data set to an signal representing a detected fluorescence intensity emitted solely by the fluorescent agent from within the medium. The disclosed method includes removing the effects of leak-through of excitation-level light and removing the effects of autofluorescence from the DRsignal.

Description

WO 40984 '1' METHOD FOR VASIVE MONITORING OF FLUORESCENT TRACER AGENT WITH BACKGROUND SEPARATION CORRECTIONS CROSS-REFERENCE TO RELATED APPLICATION This application claims the benefit of US. Provisional Application No. 62/452,021 filed January 30, 2017, which is incorporated herein in its ty.

BACKGROUND OF THE DISCLOSURE The t disclosure relates generally to methods for non-invasive monitoring of a fluorescent tracer agent within a medium characterized by scattering and/or absorption of light. More particularly, the present disclosure relates to methods for non- invasive assessment of kidney function by monitoring the clearance of an exogenous fluorescent tracer within the tissues of a patient in vivo.

Dynamic monitoring of renal function in patients at the bedside in real time is highly ble in order to minimize the risk of acute renal failure brought on by s clinical, physiological and pathological conditions. It is particularly ant in the case of critically ill or injured patients because a large percentage of these patients face the risk of multiple organ failure (MOF) incited by one or more severe dysfunctions, such as: acute lung injury (ALI), adult respiratory distress syndrome (ARDS), hypermetabolism, nsion, persistent inflammation, and/or . Renal function may also be ed due to kidney damage associated with administration of nephrotoxic drugs as part of a ure such as angiography, diabetes, auto-immune disease, and other dysfunctions and/or insults causally linked to kidney damage. In order to assess a t’s status and to monitor the severity and/or progression of renal function over extended periods, there exists considerable interest in ping a simple, accurate, and continuous method for the determination of renal failure, preferably by non-invasive procedures.

Serum creatinine concentration, an endogenous marker of renal function, is lly measured from a blood sample and used, in combination with patient demographic factors such as weight, age, and/or ethnicity to estimate glomerular filtration rate (GFR), one measure of renal function. However, creatinine-based assessments of renal function may be prone to inaccuracies due to many potential factors, including: age, state of hydration, renal perfusion, muscle mass, dietary intake, and many other anthropometric and clinical variables. To compensate for these variances, a series of creatinine-based equations (most recently extended to cystatin C) have been developed which incorporate factors such as sex, race and other relevant factors for the estimation of glomerular filtration rate (eGFR) based on serum creatinine ements. However, these eGFR equations are not provided with any means of compensating for most of the above sources of ce, and therefore have relatively poor accuracy. Further, the eGFR method typically yields results that lag behind true GFR by up to 72 hrs.

Exogenous marker compounds, such as inulin, iothalamate, 51Cr-EDTA, Gd-DTPA and 99mTc-DTPA have been used in existing methods for measuring GFR. Other endogenous markers, such as 123I and 125I labeled o-iodohippurate or 99mTc-MAG3 have been used to in other existing methods for assessing the tubular secretion process.

However, the use of typical exogenous marker nds may be accompanied by various undesirable effects including the uction of ctive materials and/or ionizing radiation into the patient, and laborious ex vivo handling of blood and urine samples, rendering existing methods using these exogenous markers unsuitable for real-time monitoring of renal on at a patient’s bedside.

The bility of a real-time, accurate, repeatable measure of renal excretion rate using exogenous markers under patient-specific yet potentially changing circumstances would represent a substantial improvement over any tly ced method. Moreover, a method that depends solely on the renal elimination of an exogenous al entity would provide a direct and continuous pharmacokinetic measurement requiring less tive interpretation based upon age, muscle mass, blood pressure, etc.

BRIEF DESCRIPTION OF THE DRAWINGS The disclosure will be better understood, and features, aspects and advantages other than those set forth above will become nt when consideration is given to the following detailed description thereof. Such detailed description makes reference to the ing drawings, wherein: is a schematic illustration of a single-wavelength renal monitoring device in one aspect, is a schematic illustration of a dual-wavelength renal monitoring system in one aspect; is a graph summarizing the absorption, transmission, and emission spectra of various devices, materials, and compounds associated with the vasive monitoring of an exogenous fluorescent agent in vivo defined over light wavelengths ranging from about 430 nm to about 650 nm, is a graph summarizing the absorption spectra of oglobin (HbOz) and deoxyhemoglobin (Hb) defined over light wavelengths ranging from about 200 nm to about 650 nm, is a schematic illustration of the timing of light pulse cycles associated with data acquisition by a dual-wavelength renal monitoring system in one aspect, in which each light pulse cycle includes light pulses produced at the tion wavelength and at the on wavelength in sequence, is a side view of a sensor head of a renal function ring system in one aspect, is a bottom view of the sensor head of is a top interior view of the sensor head of illustrating an ement of various electrical components within a housing of a sensor head of a renal function monitoring system in one aspect, is an enlargement of the interior view of is a schematic ration of the apertures formed within a contact surface of a sensor head of a renal function monitoring system in one aspect, is a schematic illustration of synchronous detection of light by a light detector of a sensor head in one aspect, is a schematic illustration of light signal modulation and demodulation by the sensor head in one aspect, A is a block diagram illustrating the ts of a processing unit in one aspect; B is a block diagram illustrating the subunits of a processing unit in a second aspect is a graph of raw cence signal as a function of time illustrating various phenomenon contributing to the total signal; is a graph of intrinsic fluorescence signals, with and without an autofluorescence correction, as a function of time illustrating the effect of an autofluorescence correction on the renal decay time constants (RDTC) d from analysis of the intrinsic fluorescence signal; is a graph of raw fluorescence signal as a function of time in which the final fluorescence signal falls below the original background fluorescence signal level due to s phenomena contributing to the total signal; A is a graph of raw fluorescence signal and excitation light leak- through as a function of time; B is a graph of raw fluorescence signal of A and a corrected fluorescence signal comprising the raw fluorescence signal with the excitation light leak- h of A removed; is a graph comparing raw fluorescence signal (blue line) and orescence signal (green line) obtained prior to injection of an exogenous fluorescent agent; A is a graph comparing raw fluorescence signal; autofluorescence signal; and diffuse reflectance signals DRexmeas= DRem; and DRemflltgrgd ed prior to ion of an exogenous fluorescent agent; B is a graph comparing raw fluorescence signal; autofluorescence signal; and diffuse reflectance signals DRexmeas= DRem; and DRemflltgrgd obtained after injection of an exogenous fluorescent agent; is a flow chart summarizing the steps of a background correction method for removing the effects of excitation-wavelength light leak-through and autofluorescence from the raw measured fluorescence signal; is a graph of entative raw fluorescence signal measurements (IFagem) detected by a renal monitoring device obtained before and after injection of an exogenous fluorescent agent; A is a block diagram illustrating a plurality of modules of a pre- processing subunit in one aspect; B is a block m illustrating a plurality of modules of a pre- processing subunit in a second aspect; is an isometric view of a sensor head of a renal on ring system in a second ; [003 5] is a bottom view of the sensor head of a renal function monitoring system rated in ; is an isometric view of the sensor head of a renal function monitoring system illustrated in with the upper housing and various electrical components removed to expose an inner housing; is an exploded view of the inner housing of the sensor head illustrated in ; is a graph showing DRexmeasand Flrmeas over a full day in the absence of administration of an ous fluorescent agent; is a graph showing DRexmeasand Flrmeas immediately preceding and following administration of an exogenous fluorescent agent; and is a graph summarizing a relationship between empirically DRem determined Flrleakthmughand derived from a database of 33 ts.

DRemFilt WO 40984 '6' This written description uses examples to disclose the invention, including the best mode, and also to enable any person skilled in the art to practice the invention, including making and using any devices or systems and ming any incorporated s. The patentable scope of the invention is defined by the claims, and may include other examples that occur to those skilled in the art. Such other examples are intended to be within the scope of the claims if they have structural elements that do not differ from the l language of the claims, or if they include lent structural elements with tantial differences from the literal languages of the claims.

DETAILED DESCRIPTION Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ry skill in the art to which the disclosure belongs. Although any methods and materials similar to or equivalent to those described herein may be used in the practice or testing of the present disclosure, the preferred materials and methods are described below.

A sample, as used herein, refers to a single, discrete data value acquired from a signal and/or telemetry -to-digital converter (ADC) for a single acquisition/telemetry channel.

A measured value, as used herein, refers to a single, discrete data value created by demodulating or accumulating a sequence of samples from one acquisition channel.

A measurement, as used herein, refers to a set comprising the Demodulated In-Phase, lated Out-of—Phase, and Averaged measurement values from one acquisition channel.

A measurement subset, as used herein, refers to a set comprising all measurements for all ition channels during a single source LED nation. For example, all measurements of an acquisition channel may include demodulated in-phase, demodulated out-of-phase, and averaged measurements.

A measurement set, as used herein, refers to a set comprising one measurement subset for each source LED. '7' 2018/016053 An acquisition, as used herein, refers to the overall s by which a measurement set is obtained.

A measurement sequence, as used herein, refers to a sequence of one or more measurement sets.

A telemetry value, as used herein, refers to a single, discrete data value acquired from a single channel of a telemetry ADC.

A try set, as used herein, refers to a set comprising one telemetry value from each telemetry channel.

A diffuse reflecting medium, as used herein, refers to any material through which light propagates, which includes a plurality of moieties, particles, or molecules that may scatter, reflect, and/or absorb the light as it propagates. The bution of the plurality of moieties, particles, and/or molecules may be m or non-uniform, and may change over time.

In various s, systems and methods for monitoring time-varying fluorescence d from a fluorescent agent from within a diffuse reflecting medium with time-varying optical properties are disclosed herein below. In one aspect, systems and methods for monitoring a time-varying fluorescence emitted from an exogenous fluorescent agent within the tissues of a patient are disclosed. The systems and methods of this one aspect may be used in a variety of contexts including, but not limited to, the monitoring of renal function in vivo in a patient in real time by monitoring the decreasing fluorescence emitted by an exogenous fluorescent agent within the tissue of a patient as the exogenous fluorescent agent is eliminated by the kidneys of the patient. Although the systems and devices disclosed herein below are described in the context of methods and devices to monitor kidney function, it is to be understood that the sed systems and methods may be applied to any s and methods that r the arying fluorescence emitted by a fluorescent agent from within a diffuse reflecting , in which the optical ties of the diffuse reflecting medium may also vary with time. is a schematic illustration of a system 100, provided as a non-limiting example, in which fluorescence 102 with an emission wavelength (hem) is detected from a region of interest of a t 104 using a light detector 110 configured to detect only those photons with an emission wavelength (hem). In general, the exogenous fluorescent agent 112 produces fluorescence 102 in se to an excitation event ing, but not limited to: nation by light 106 at an excitation wavelength (hex), occurrence of an enzymatic reaction, changes in local ical potential, and any other known excitation event associated with exogenous fluorescent agents. In an aspect, the system 100 may include a light source 108 configured to deliver light 106 at an excitation wavelength (hex) to the patient 104. In this aspect, the fluorescence 102 is produced in response to illumination by the light 106. In addition, the excitation wavelength (hex) of the light 106 and the emission wavelength (hem) of the fluorescence 102 are ally ct (i.e., hex is sufficiently ent from hem) so that the light detector 110 may be configured to selectively detect only the fluorescence 102 by the inclusion of any known optical wavelength separation device including, but not d to, an optical filter.

In some s, changes in the fluorescence 102 may be monitored to obtain information regarding a physiological function or status of the patient. By way of non-limiting example, the time-dependent decrease in the fluorescence 102 ed after introduction of the exogenous fluorescent agent 112 into a circulatory vessel of the patient 104 may be analyzed to obtain information regarding renal function of the t 104. In this non-limiting example, the rate of decrease in cence 102 may be assumed to be proportional to the rate of l of the exogenous fluorescent agent 112 by the kidneys of the patient 104, thereby providing a measurement of renal function including, but not limited to: renal decay time constant (RDTC) and glomerular filtration rate (GFR).

Without being limited to any particular theory, the intensity of fluorescence 102 detected by the light detector 110 may be influenced by any one or more of numerous factors including, but not limited to: the intensity or power of the light 106 at hex delivered to the patient 104, the scattering and absorption of the light 106 passing through intervening tissues 114 of the patient 104 between the light source 108 and the exogenous fluorescent agents 112, the concentration of exogenous fluorescent agents 112 illuminated by the light 106, the scattering and absorption of the fluorescence 102 at hem passing through intervening tissues 114 of the patient 104 n the exogenous fluorescent agents 112 and the light detector 110, leak-through of the excitation light 106 through any optical filters configured to transmit only light at emission wavelength hem, and fluorescence emitted by endogenous skin ents. is a graph showing a representative time y of a raw fluorescence signal obtained at the emission wavelength hem corresponding to the wavelength of cence emitted by an endogenous fluorescent agent within the tissues of a patient in response to illumination by tion-wavelength light. The measured raw fluorescence signal obtained prior to the injection of the endogenous fluorescence agent (i.e. the background signal 1402) may include autofluorescence (Fauw) emitted by endogenous ures as well as leak-through of tion-wavelength light (ExLI) through any optical filters configured to transmit only emission-wavelength light to the light detector producing the raw cence signal. The measured raw fluorescence signal obtained after the ion of the endogenous fluorescent agent (i.e. Flrmeas 1404) may include the ity of the fluorescence emitted by the endogenous fluorescent agent (Fagem) superimposed over the background signal 1402 (i.e. FWD and ExLI).

Existing methods typically assume that the optical properties within the intervening tissue 114 remain essentially unchanged throughout the period during which ements are obtained by the system 100. As a result, existing methods typically obtain l measurements through the intervening tissue 114 of the patient 104 prior to introduction of the exogenous fluorescent agent 112, and these initial measurements are subtracted to correct all subsequent data obtained after introduction of the exogenous fluorescent agent 112. However, during long-term monitoring of the patient 104, changes in the optical properties of the intervening tissue 114 may occur due to changes in at least one characteristic ing, but not limited to: optical coupling efficiency of the light detector 110 to the patient 104, concentration of chromophores such as hemoglobin due to changes in blood volume caused by vascular dilation, iction, or compression, changes in the optical properties of chromophores such as hemoglobin due to changes in oxygenation status, and changes in tissue structure such as changes related to edema. is a graph of the raw fluorescence signal measured before and after the injection of an endogenous fluorescent agent, illustrating that the background signal may change over the extending data ition period associated with the renal clearance of the endogenous fluorescent agent from the patient. As illustrated in , the initial W0 2018/140984 PCT/U82018/016053 background signal level 1602 is about 0.01 intensity units higher than the final background signal level 1604 measured about nine hours after the measurement of the initial background signal level 1602. Without being limited to any particular theory, it is thought the administration of a blood pressure medication during the data ition period may have induced skin flushing and associated vasodilation of skin aries that may have altered the l properties of the patient’s skin, due to the increased concentration of blood, which contains hemoglobin, an known endogenous chromophore capable of absorbing light at both the excitation and emission wavelengths.

These dynamic changes in the optical properties of the ening tissue 114 may introduce uncertainty into long-term measurements of fluorescence 102. By way of miting example, changes in the optical properties of the intervening tissue 114 may modulate the intensity or power of the light 106 illuminating the exogenous fluorescent agents 112, causing a modulation of the fluorescence 102 produced by the exogenous fluorescent agents 112 that may be erroneously interpreted as a tion in the tration of the ous cent agents 112. By way of r miting example, changes in the optical properties of the intervening tissue 114 may modulate the intensity or power of the fluorescence 102 reaching the light detector 110 that may also be erroneously reted as a modulation in the concentration of the exogenous fluorescent agents 112. The potential modulation of changes in the optical properties of the intervening tissue 114 may uce ainty into measurements of fluorescence 102, in particular those measurements associated with long-term monitoring of fluorescence 102 as described herein above.

Similarly, because autofluorescence (Fame) produced by endogenous chromophores occurs in a similar manner to the fluorescence produced by the exogenous fluorescent agent, dynamic changes in the optical properties of the intervening tissue may introduce variability in the autofluorescence (Fame) levels over the course of long-term measurements of fluorescence 102. By way of non-limiting example, changes in the scattering and absorption of the light 106 passing through the intervening tissue 114 may modulate the intensity or power of the light 106 illuminating the endogenous chromophores, causing a modulation of the autofluorescence that may modulate the background fluorescence over the course of data acquisition. By way of another non- limiting example, changes in the scattering and absorption of the autofluorescence passing W0 2018/140984 PCT/U82018/016053 through the intervening tissue 114 may modulate the intensity of the autofluorescence detected by the light detector 110 that may te the background fluorescence over the course of data acquisition. The potential modulation of background cence, if not ly accounted for, may introduce uncertainty into raw fluorescence measurements and by extension may introduce uncertainty into parameters derived from an analysis of these florescence measurements.

By way of non-limiting example, changes in autofluorescence d to dynamic changes in the optical properties of the skin of the patient may introduce uncertainty into the calculation of renal decay time constant (RDTC), a measure of renal function as bed herein below. is a graph of a raw fluorescence signal measured before and after the injection of an endogenous fluorescent agent that includes autofluorescence (IFAgent+Aut0F;r, blue line). The graph of also includes a corrected fluorescence signal m, green line) calculated by removing the effects of autofluorescence from the raw fluorescence signal using the methods described herein below. Superimposed on each signal are flts associated with the calculation of RDTC. As shown in , the RDTC value of 2.76 hr. ated using the raw fluorescence signal is considerably higher that the corresponding RDTC value of 2.31 hr. calculated using the corrected fluorescence signal.

In various aspects, a method of correcting in vivo real-time measurements of fluorescence from an exogenous fluorescent agent to remove the effects of changes in the optical properties within the tissue of the patient is provided. The inclusion of an additional measurement of light passing through the tissue of the patient via a separate optical pathway (i.e. diffuse reflectance) from the optical pathway of the fluorescence measurements enhanced the quantification of changes in the optical properties of the tissue during prolonged monitoring of fluorescence from an exogenous fluorescent agent within a patient. The inclusion of this additional measurement in the correction method in s s was discovered to significantly enhance the y of cence measurements.

Detailed descriptions of devices for monitoring the fluorescence of an ous fluorescent agent in vivo and methods of correcting the fluorescence measurements to remove the effects of changes in the background signal are provided herein below.

W0 2018/140984 PCT/U82018/016053 Although the devices and methods are described herein below in the t of a non-invasive optical renal function monitor, it is to be understood that the correction method described herein, with appropriate modification, may be applied to any compatible device configured to m ements by delivering EM radiation from an external source through any scattering medium and/or ing EM radiation propagated h any scattering medium to an external detector. Non-limiting examples of EM radiation include visible light, near-IR light, IR light, UV radiation, and microwave radiation. The scattering media may include any living or non-living material capable of propagating EM radiation of at least one EM frequency without tion. At least a portion of the scattering media may further include one or more substructures or compounds capable of ing and/or absorbing the EM radiation. Non-limiting examples of scattering media include: a tissue of a living or dead organism, such as a skin of a mammal, a gas such as air with or without additional particles such as dust, fluid droplets, or a solid particulate material, a fluid such as water with or without onal particles such as gas bubbles or a solid particulate material. r, the devices and methods described herein below are not limited to detection of renal function, but may be modified for use in the detection of the function of other physiological systems including, but not limited to, liver systems, or gastro-intestinal s.

System Description In various aspects, the methods of correcting fluorescence measurements to remove the s of variations in local skin properties as disclosed herein may be orated into any fluorescence monitoring system including, but not limited to, a system for optically monitoring renal function in vivo and in real time by ing changes in fluorescence of an exogenous fluorescent agent injected into a patient as the agent is renally eliminated from the patient. is a block diagram of a system 200 for optically monitoring renal function of a patient 202 via measurements of the fluorescence of an injected exogenous fluorescent agent in the patient 202, in one aspect. The system 200 may include at least one sensor head 204 configured to deliver light at an excitatory wavelength (hex) into a first region 206 of the patient 202. The system 200 is further configured to detect light at an emission wavelength (hem), at a second region 208 of the patient 202, and to detect light at the excitatory wavelength (hex), and/or emission wavelength (hem), at a third region 210 of the patient 202.

W0 2018/140984 PCT/U82018/016053 The system 200 may further include a controller 212 operatively d to the at least one sensor head 204, an ion unit 214, and a display unit 216. In various s, the controller 212 is red to control the operation of the at least one sensor head 204 as described in additional detail herein below. The controller 212 is further configured to receive measurements of light from the at least one sensor head 204. The controller 212 is further configured to correct the light measurements corresponding to fluorescence from exogenous fluorescent agents according to at least one method including, but not limited to, the disclosed methods of correcting fluorescence measurements using measurements indicative of dynamic changes in the background signal related to changes in autofluorescence and/or the leak-through of excitatory-wavelength light to the second light detector 224 configured to detect on-wavelength light only.

The controller 212 is further configured to transform the fluorescence measurements received from the at least one sensor head 204 into a summary parameter representative of the renal function of the t 202. In addition, the controller 212 is red to receive at least one signal representing user inputs from the operation unit 214 and to generate one or more forms for display on the display unit 216 including, but not limited to, a graphical user ace (GUI).

A detailed description of the sensor head 204 and controller 212 are provided herein below.

A. Sensor Head In various aspects, the sensor head 204 includes at least one light source and at least one light detector in a housing. is a side view of a g 600 for the sensor head 204 in one aspect that includes an upper housing 602 and a lower housing 604 attached together to enclose two light sources and two light detectors. The bottom surface 608 of the lower housing 604 further includes a contact surface 606 configured to be ed to the skin of a patient 202 using a patible adhesive material including, but not limited to, a surgical adhesive. In use, the surface of the adhesive material opposite to the contact surface 606 may be affixed to the skin of the patient 202. In various aspects, the adhesive material may be configured to transmit light through the light s into the patient and to further transmit the fluorescence from the patient to the light detectors. In one aspect, the adhesive material may be an optically transparent al. In another W0 2018/140984 aspect, the ve material may be produced from a non-fluorescing material to prevent the production of confounding fluorescence by the adhesive material.

In various other aspects, the upper g 602 may r include one or more openings 806 configured to provide access to the interior for a cable including, but not limited to, a USB cable, and/or to e a window for a y generated by the circuitry contained within the housing 600, such as an indicator LED. is a bottom view of the housing 600 illustrated in The contact surface 606 may include an aperture plate 702 including one or more apertures 704 configured to transmit light between the skin of the t and the light s and light detectors contained inside the housing 600. In one aspect, the aperture plate 702 may be epoxied into the lower housing 604 to prevent liquid ingress into the interior of the housing 600. In various aspects, the dimensions, arrangement, and/or spacing of the one or more apertures 704 may be selected to enhance various aspects of the operation of the system 200, as described in additional detail herein below. In another , the t surface 606 may further include a temperature sensor opening 706 configured to provide a thermal path from the skin surface of the t to an additional temperature sensor 228 configured to monitor the temperature at the skin surface of the patient. is a schematic m illustrating the arrangement of the electrical components within the housing 600. Referring to the upper housing 602 and the lower housing 604 may be affixed together with screws 802, and the screw holes and the interface between the two housing pieces may be filled with a resistant filler material 804 including, but not limited to, a silicone material such as room temperature vulcanization silicone (RTV) to inhibit liquid ingress into the interior of the housing 600.

In an aspect, the housing 600 may further include a cable opening 806 formed through the upper housing 602. The cable opening 806 may be configured to provide access to the interior for an electrical cable including, but not limited to, a USB cable. In one , the cable may enable the supply of power to the light sources, light detectors, tor lights, and associated electrical devices and circuits as described herein below. In another , the cable may further enable the communication of control signals into the housing to enable the operation of the electrical components within the housing 600, and the cable may further enable the communication of data signals encoding W0 2018/140984 measurements obtained by one of more of the sensor devices contained within the housing 600 ing, but not limited to: the first light detector 222, the second light detector 224, any additional light detectors, such as a first monitor iode 904 and a second monitor diode 906, and any additional temperature sensors 228 (see . In an aspect, the cable may be attached to the cable opening 806 and adjacent upper housing 602 with a light absorbent adhesive including, but not limited to, black epoxy and may further be sealed against water incursion using a water resistant filler material including, but not limited to, RTV.

In an additional aspect, the housing 600 may further include at least one display opening 808 formed through the upper housing 602. In one aspect, each display opening 808 may be configured to e a window for a display generated by the try contained within the housing 600, such as an indicator LED 810. In an aspect, each indicator LED 810 may be oned on a circuit board 812. In an aspect, a light pipe 814 may be epoxied into the display opening 808 within the upper housing 602 above each indicator LED 810. Each a light pipe 814 may be filled with a water-resistant filler material such as RTV for liquid ingress protection. In s aspects, the at least one tor LED 810 may illuminate in a predetermined pattern to enable a user of the system 200 to r the operational status of the sensor head 204. is a close-up view of the interior optical region of the sensor head 204 g the arrangement of the light sources 2l8/220 and the light detectors 222/224 within the housing 600 in one aspect. In an aspect, the light sources 2l8/220 are separated from the light detectors 222/224, and the first light detector 222 is separated from the second light detector 224 are separated from one another by a sensor mount 9l2 affixed to the aperture plate 702. In an aspect, the sensor mount 9l2 ensures that light from the light sources 2l8/220 does not reach the light detectors 222/224 without coupling h the skin of the t 202. The separation between the first light detector 222 within the first detection well 908 and the second light detector 224 within the second detection well 910 ensures that the fluorescence signal produced by the exogenous fiuorescent agent within the tissues of the patient 202 is distinguishable from the unfiltered excitation light introduced by the first light source 218.

W0 2018/140984 Referring again to the sensor mount 912 may be aligned to a circuit board (not shown) containing the light sources 0 and light detectors 222/224 using alignment pins 914 and held in place using screws 916. In an aspect, the sensor mount 912 may be affixed to the circuit board containing the light s 218/220 and light detectors 222/224 using a light absorbent adhesive including, but not d to, black epoxy. In this aspect, this light-resistant join between the circuit board and the sensor mount 912 inhibits leakage of light between the light sources 218/220 and the light detectors 222/224, and further inhibits the leakage of light between the first light detector 222 and the second light detector 224. The apertures 704 configured to transmit light to and from the skin underlying the contact surface 606 of the sensor head 204 are formed through a structurally te aperture plate 702 (see to provide for precise alignment of the apertures 704 to the corresponding light s 0 and light detectors 222/224, described in additional detail herein below.

In various aspects, the sensor mount 912 may further provide electrical shielding for any sensitive electrical devices within the sensor head 204 ing, but not limited to, the light detectors 222/224. In one aspect, the sensor mount 912 may be constructed of an electrically conductive material including, but not limited to: aluminum and aluminum alloy. In this aspect, the sensor mount 912 may be electrically coupled to the ground of the circuit board using conductive screws 916. In on, any glass windows positioned within the source well 902 and/or or wells 908/910 adjacent to the aperture plate 702 including, but not limited to, an optical filter 244 and clear glass 246 as described herein below (see may further include an electrically conductive coating.

Non-limiting examples of suitable electrically conductive coatings for the glass windows of the sensor mount include a conductive indium tin oxide (ITO) coating and any other suitable transparent and electrically conductive coating.

Without being d to any particular theory, the conductive material of the sensor mount 912 provides a l Faraday cage to shield the electrically sensitive detectors 222/224 from electrical noise generated by or conducted through the patient’s body. The partial Faraday cage ed by the sensor mount 912 may be completed with the conductive ITO g on the glass windows within the source well 902 and/or detector wells 908/910. In an aspect, the electrically conductive coating on the glass windows, such as an ITO coating, are sufficiently conductive to provide ical shielding W0 2018/140984 while remaining sufficiently transparent for the transmission of light to and from the skin surface of the patient 202. In another , the ITO coating of each glass window may be grounded to an ically conductive sensor mount 912 using any known electrical grounding method including, by not limited to: a wire ting the glass coating to the sensor mount 912 that is attached at both wire ends with conductive epoxy, or attaching the coated glass directly to a glass fitting such as a ledge or frame formed within each of the source well 902 and/or detector wells 908/910 using an electrically conductive epoxy.

In various aspects, the contact surface 606 of the g 600 may be attached the patient’s skin using a patible and an adhesive material 610 ing, but not limited to, a clear double-sided medical grade adhesive, as illustrated in and Any adhesive material selected to be optically transmissive at the excitation and emission wavelengths used by the system 100 as described herein. The adhesive material 610 may be positioned on the contact surface 606 such that the adhesive material covers the apertures 704, but exposes the temperature sensor opening 706 to ensure sufficient thermal contact with the skin of the patient 202. In one aspect, the sensor head 204 may be further secured to the patient 202 as needed using one or more additional patible medical fastener devices including, but not limited to: Tegaderm bandages, medical tape, or any other suitable biocompatible medical er devices.

In an aspect, the contact surface 606 may be d near the front edge of the sensor head 204 to provide for te positioning of the contact surface 606 on a ed region of the patient’s skin. In another aspect, the apertures 704 may be positioned towards the center of the contact surface 606 to reduce ambient light ingress. Without being limited to any particular , ambient light may enter one or more of the apertures 704 due to incomplete on of the contact surface 606 to the patient’s skin and/or due to the propagation of ambient light passing through the patient’s exposed skin situated just outside of the footprint of the contact surface 606 into the apertures 704.

Referring again to the bottom surface 608 of the sensor head 204 curves away from the plane of the contact surface 606 to enable attachment of the sensor head 204 to varied body type and locations. For attachment of the sensor head 204 to relatively flat or concave surfaces, any gap 612 between the bottom surface 608 and the W0 2018/140984 skin surface of the patient 202 may be filled with a biocompatible foam to ensure consistent contact with the patient 202. 1) Light sources In various aspects, each sensor head 204 includes a first light source 218 and a second light source 220 configured to r light to a first region 206 of a patient 202. The first light source 218 is configured to deliver the light at the excitatory wavelength and the second light source 220 is configured to r light at the emission ngth. In one aspect, the excitatory wavelength may be selected to fall within a spectral range at which the exogenous cent agent ts relatively high absorbance. In another aspect, the on wavelength may be selected to fall within a spectral range at which the exogenous fluorescent agent exhibits relatively high emission.

The exogenous fluorescent agent may be selected for enhanced contrast ve to other chromophores within the tissues of the patient 202 including, but not limited to hemoglobin within red blood cells and/or melanin within melanocytes. In various aspects, the exogenous fluorescent agent may be selected to conduct measurements within spectral ranges with lower variation in absorption by other chromophores such as hemoglobin within the tissues of the patient 202 during use.

Without being limited to any particular theory, hemoglobin (Hb) is an absorber of visible light in the tissues of the patient 202, and has the potential to interfere with the measurements of fluorescence of the exogenous fluorescent agent if the Hb absorbance varies over the measurement period of the system 200. Because hemoglobin (Hb) enables gas exchange within virtually all tissues containing circulatory vessels, virtually all tissues are vulnerable to interference with fluorescence measurements of the system 200 due to fluctuations in hemoglobin tration. Within most tissues, externally applied pressure may cause blood g which may be manifested as an apparent decay of the fluorescence measured at the skin surface. Periodic opening and closing of blood vessels motion”) near the surface of the skin may also cause fluctuations in hemoglobin concentration which may introduce additional noise in to ements of fluorescence of the exogenous cent agent by the system 200. r, in some patients 202, such as those with pulmonary disorders, variation in the Hb oxygenation state may also be observed, leading to additional potential variations in the W0 2018/140984 background skin absorbance due to differences in the absorption spectra of deoxyhemoglobin (Hb) and oxyhemoglobin (HbOz), shown rated in In an aspect, the excitation and emission wavelengths for the exogenous fluorescent agent may be ed to coincide with a pair of HbOz/Hb stic points, each isosbestic point defined herein as a wavelength characterized by about equal light absorbance by HbOz and Hb. Without being limited to any ular theory, fluorescence measurements conducted at each isosbestic wavelength are less sensitive to variation due to changes in the oxygenation of obin, so long as the ed concentration of HbOz and Hb remains relatively stable during measurements of fluorescence by the system 200.

Non-limiting examples of Hb/HbOz isosbestic wavelengths include: about 390 nm, about 422 nm, about 452 nm, about 500 nm, about 530 nm, about 538 nm, about 545 nm, about 570 nm, about 584 nm, about 617 nm, about 621 nm, about 653 nm, and about 805 nm.

In various aspects, the excitation and emission wavelengths may be ed based on the tion and emission wavelengths of the selected exogenous fluorescent agent of the system 200. In one aspect, the excitatory wavelength may be an HbOz/Hb isosbestic wavelength and simultaneously may be a wavelength within a spectral range of high absorbance of the exogenous fluorescent agent. In another aspect, the emission wavelength may be an HbOz/Hb isosbestic wavelength and simultaneously may be a ngth within a spectral range of emission by the exogenous fluorescent agent. Table 1 provides a summary of b isosbestic wavelengths within the spectral range of 200 nm to about 1000 nm. is a graph of the absorption spectra used to identify the HbOz/Hb isosbestic wavelengths of Table 1.

Table I. Hb02/Hb Isosbesll'c Wavelengths )t = 200 - 1000 nm Excitation Hb Molar Hb02 dA/d)» Hb dA/dl Wavelength Extinct. Coeff. (M'1 cm'1 nm'l) W0 2018/140984 By way of rative example, is a graph izing the absorption a for HbOz and Hb, as well as the absorption and on spectra of frequency spectra of 1Vfl3402, an exogenous fluorescent agent in one aspect. Emission spectra for a blue LED light source and a green LED light source are also shown superimposed over the other spectra of In this , the system 200 may include a blue LED as the first light source 218, and the excitatory ngth for the system 200 may be the isosbestic wavelength of about 450 nm. As listed in Table 1 and shown in the Hb absorbance spectra is strongly sloped at the isosbestic wavelengths of about 420 nm to about 450 nm (see columns 3 and 4 of Table 1), indicating that the relative absorbance of HbOz and Hb at the isosbestic wavelength of about 450 nm is sensitive to small changes in excitatory wavelength. However, at wavelengths above about 500 nm, the HbOz/Hb spectra are less steeply sloped, and a broader band light source including, but not d to, an LED with a bandpass filter may suffice for use as a first light source 218.

In another aspect, the excitatory wave length may be selected to enhance the contrast in light absorbance between the exogenous fluorescent agent and the chromophores within the tissues of the patient 202. By way of non-limiting example, as shown in at the isosbestic ngth of 452 nm, the light absorption of the MB- 102 is more than three-fold higher than the light absorption of the HbOz and the Hb.

Without being limited to any particular theory, a higher proportion of light illuminating the tissue of the patient 202 at a wavelength of about 450 nm will be absorbed by the 1Vfl3402 relative to the HbOz and Hb, thus enhancing the efficiency of absorption by the 1Vfl3402 and reducing the intensity of light at the excitatory wavelength needed to elicit a detectable fluorescence signal.

In various aspects, a second isosbestic wavelength may also be selected as the emission wavelength for the system 200. By way of miting example, shows an emission spectrum of the MB402 exogenous contrast agent that is characterized W0 2018/140984 PCT/U82018/016053 by an emission peak at a wavelength of about 550 nm. In this non-limiting example, the isosbestic wavelength of 570 nm may be selected as the emission ngth to be detected by first and second detectors 222/224. In various other aspects, the emission wavelength of the system 200 may be ed to fall within a spectral range characterized by vely low absorbance of the chromophores within the tissues of the patient 202.

Without being d to any particular theory, the low absorbance of the chromophores at the selected emission wavelength may reduce the losses of light emitted by the exogenous fluorescent agent and enhancing the efficiency of fluorescence detection.

In various aspects, the first light source 218 and the second light source 220 may be any light source configured to deliver light at the excitatory wavelength and at the emission wavelength. Typically, the first light source 218 delivers light at an ity that is sufficient to penetrate the s of the t 202 to the exogenous fluorescent agent with sufficient intensity remaining to induce the emission of light at the emission wave length by the exogenous fluorescent agent. Typically, the first light source 218 rs light at an intensity that is sufficient to penetrate the tissues of the patient 202 to the exogenous fluorescent agent with sufficient intensity remaining after scattering and/or absorption to induce fluorescence at the emission wave length by the exogenous fluorescent agent. However, the intensity of light delivered by the first light source 218 is limited to an upper value to prevent adverse effects such as tissue burning, cell damage, and/or photo-bleaching of the exogenous fluorescent agent and/or the endogenous chromophores in the skin (“auto-fluorescence”). rly, the second light source 220 rs light at the emission wavelength of the exogenous fluorescent agent at an intensity red to provide sufficient energy to propagate with scattering and tion through the first region 206 of the patient and out the second region 208 and third region 210 with sufficient remaining intensity for detection by the first light detector 222 and the second light detector 224, respectively. As with the first light source 218, the intensity of light ed by the second light source 220 is limited to an upper value to prevent the adverse effects such as tissue injury or photobleaching described previously.

W0 40984 2018/016053 In various aspects, the first light source 218 and the second light source 220 may be any light source suitable for use with fluorescent medical imaging systems and devices. Non-limiting examples of suitable light sources include: LEDs, diode lasers, pulsed lasers, continuous waver lasers, xenon arc lamps or mercury-vapor lamps with an excitation filter, lasers, and supercontinuum sources. In one aspect, the first light source 218 and/or the second light source 220 may produce light at a narrow spectral bandwidth suitable for monitoring the concentration of the exogenous fluorescence agent using the method described herein. In another , the first light source 218 and the second light source 220 may produce light at a relatively wide spectral bandwidth.

In one , the selection of intensity of the light produced by the first light source 218 and the second light source 220 by the system 200 may be influenced any one or more of at least several factors including, but not limited to, the maximum permissible exposure (MPE) for skin exposure to a laser beam according to applicable regulatory standards such as ANSI rd Z1361 In another aspect, light intensity for the system 200 may be selected to reduce the likelihood of photobleaching of the exogenous fluorescent source and/or other chromophores within the tissues of the patient 202 including, but not limited to: collagen, keratin, elastin, hemoglobin within red blood cells and/or melanin within melanocytes. In yet another aspect, the light intensity for the system 200 may be selected in order to elicit a detectable fluorescence signal from the exogenous fluorescent source within the tissues of the t 202 and the first light detector 222 and/or second light detector. In yet r aspect, the light intensity for the system 200 may be selected to provide ly high light energy while ng power consumption, inhibiting heating/overheating of the first light source 218 and the second light source 220, and/or reducing the exposure time of the patient’s skin to light from the first light detector 222 and/or second light detector.

In s aspects, the intensity of the first light source 218 and the second light source 220 may be modulated to compensate any one or more of at least several factors including, but not limited to: individual differences in the tration of phores within the patient 202, such as variation in skin pigmentation. In various other aspects, the detection gain of the light detectors may be modulated to similarly sate for variation in individual differences in skin properties. In an aspect, the variation in skin pigmentation may be between two different individual patients 202, or W0 2018/140984 PCT/U82018/016053 between two different ons on the same patient 202. In an aspect, the light modulation may compensate for variation in the optical pathway taken by the light through the tissues of the patient 202. The optical pathway may vary due to any one or more of at least several factors including but not limited to: variation in separation distances between the light sources and light detectors of the system 200; variation in the secure attachment of the sensor head 204 to the skin of the patient 202; variation in the light output of the light sources due to the exposure of the light sources to environmental factors such as heat and moisture; variation in the sensitivity of the light detectors due to the exposure of the light ors to environmental factors such as heat and moisture; modulation of the duration of illumination by the light sources; and any other relevant operational ter.

In various aspects; the first light source 218 and the second light source 220 may be configured to modulate the ity of the light produced as needed according to any one or more of the factors described herein above. In one aspect; if the first light source 218 and the second light source 220 are devices configured to continuously vary output fluence as needed; for example LED light s; the intensity of the light may be modulated onically using methods including; but not d to; modulation of the electrical potential; current; and/or power supplied to the first light source 218 and/or the second light source 220. In another aspect; the intensity of the light may be ted using optical s including; but not limited to: partially or fully occluding the light leaving the first light source 218 and the second light source 220 using an optical device including; but not limited to: an iris; a shutter; and/or one or more ; diverting the path of the light leaving the first light source 218 and the second light source 220 away from the first region 206 of the patient using an optical device including; but not limited to a ; a mirror; and/or a prism.

In various s; the intensity of the light produced by the first light source 218 and the second light source 220 may be modulated via control of the laser fluence; defined herein as the rate of energy within the produced light beam. In one aspect; the laser fluence may be d to ranges defined by safety standards including; but not limited to; ANSI standards for exposure to laser energy such as ANSI Zl36.l. Without being limited to any particular theory; the maximum fluence of light delivered to a patient 202 may be influenced by a variety of factors including; but not limited to the wavelength of the delivered light and the duration of exposure to the light. In various aspects; the maximum W0 2018/140984 fluence of light may range from about 0.003 J/cm2 for light at delivered at wavelengths of less than about 302 nm to about 1 J/cm2 for light delivered at wavelengths ranging from about 1500 nm to about 1800 nm for a duration of up to about 10 sec. For light delivered at wavelengths ranging from about 400 nm to about 1400 nm (visible/NIR light) the maximum fluence may be about 0.6 J/cm2 for a duration of up to about 10 sec, and up to about 0.2 J/cm2 for a duration ranging from about 10 sec to about 30,000 sec. For extended exposures, the delivered light is limited to a maximum power density (W/cm2) ing to ANSI standards: visible/NIR light is limited to 0.2 W/cm2 and far IR light is limited to about 0.1 W/cm2. Without being limited to a particular theory, ed exposure to light delivered at UV wavelengths is not typically recommended according to ANSI standards.

In another aspect, the fluence of light at the excitatory wavelength produced by the first light source 218 may be modulated in order to provide sufficient energy to propagate through the skin in the first region 206 of the patient 202 to the exogenous fluorescent agent without photobleaching, and to nate the exogenous fluorescent agent with energy sufficient to induce able fluorescence at the first light detector 222 and/or the second light detector 224. In an additional aspect, the fluence of light at the emission wavelength produced by the second light source 220 may be modulated in order to provide sufficient energy to propagate through the skin in the first region 206 of the patient 202 and through the skin in the second region 208 and the third region 210 t photobleaching to emerge as detectable light at the first light detector 222 and the second light or 224, respectively. By way of non-limiting example, the fluence of light produced by a light source at 450 nm or 500 nm may be limited to 1.5 and 5 mW/cm2, tively, to prevent photo-bleaching.

In s aspects, the fluence of the light produced by the first light source 218 and the second light source 220 may be modulated by any suitable systems and/or s without limitation as described herein above. This modulation may be enabled a single time during operation of the system 200, and as a result, the fluence of the light produced by each of the first light source 218 and the second light source 220 may be relatively constant throughout the operation of the system 200. In another aspect, the light modulation may be enabled at te times over the duration of operation of the system W0 2018/140984 PCT/U82018/016053 200, or the light tion may be enabled continuously over the duration of operation of the system 200.

In one aspect, the fluence of the light may be modulated via manual adjustment of any of the power source settings and/or optical device settings as described above when the system 200 is configured in an Engineering Mode. In another aspect, the fluence of the light may be modulated tically via one or more control schemes encoded in the light source control unit of the controller 212 as described herein below. In this , the degree of modulation may be ed at least in part on the basis of feedback measurements obtained by various sensors provide in the sensor head 204 of the system 200 including, but not limited to, additional light detectors 226 and temperature sensors 228 as described in additional detail herein below.

In s aspects, light produced by the first light source 218 and the second light source 220 are further characterized by a pulse width, defined herein as the duration of the produced light. Although pulse width is typically used to characterize the performance of a light source that produces light in te pulses, such as a pulsed laser, it is to be understood that the term “light , as used herein, refers to any discrete burst of light produced by a single light source at a single wavelength to enable the acquisition of a single measurement of fluorescence by the system 200. Similarly, the term “pulse width”, as used herein, refers to the duration of a single light pulse produced by a single light source. The pulse width is typically selected based on one or more of at least several factors ing, but not limited to: delivery of sufficient light energy to elicit detectable fluorescence from the exogenous fluorescent agent without photobleaching the exogenous fluorescent agent or other chromophores within the tissues of the t 202, compliance with safety standards for light ry to patients such as ANSI rds, light delivery at sufficiently high rate to enable data acquisition at a rate compatible with real-time monitoring of renal function, performance capabilities of the selected light sources, light detectors, and other devices of the system 200, preservation of the working life of light sources, light detectors, and other devices related to producing and detecting light energy, and any other relevant factors.

W0 2018/140984 PCT/U82018/016053 In various aspects, the pulse width of the light produced by the first light source 218 and the second light source 220 may be independently selected to be a on ranging from about 0.0001 seconds to about 0.5 seconds. In various other aspects, the pulse width of the light produced by the first light source 218 and the second light source 220 may be ndently selected to be a duration ranging from about 0.0001 seconds to about 0.001 s, from about 0.0005 seconds to about 0.005 s, from about 0.001 seconds to about 0.010 seconds, from about 0.005 seconds to about 0.05 seconds, from about 0.01 seconds to about 0.1 seconds, from about 0.05 seconds to about 0.15 seconds, from about 0.1 seconds to about 0.2 seconds, from about 0.15 seconds to about 0.25 seconds, from about 0.2 seconds to about 0.3 seconds, from about 0.25 s to about 0.35 seconds, from about 0.3 seconds to about 0.4 seconds, from about 0.35 seconds to about 0.45 seconds, and from about 0.4 seconds to about 0.5 seconds. In one aspect, the pulse widths of the light produced by the first light source 218 and the second light source 220 are both about 0.1 seconds, as illustrated schematically in In another , the light produced by the first light source 218 and the second light source 220 may be further characterized by a pulse rate, defined herein as the number of pulses produced by a light source per second. Although pulse rate is typically used to characterize the performance of a light source that produces light in discrete pulses, such as a pulsed laser, it is to be understood that the term “pulse rate”, as used herein, refers to the rate of production of a discrete light pulse by a single light source at a single wavelength in association with the ition of measurements of fluorescence by the system 200. In various aspects, the pulse rate may be selected based on one or more of at least several factors including, but not limited to: compliance with safety standards for light delivery to patients such as ANSI standards, the performance capabilities of the selected light sources, light detectors, and other devices of the system 200, light delivery rates compatible with data acquisition rates sufficiently rapid for ime monitoring of renal function, preserving the working life of light sources, light detectors, and other devices related to producing and detecting light energy, and any other relevant factor.

In various s, the light sources are configured to deliver light into the tissues of the patient 202 at a single position such as a first region 206, rated schematically in In one aspect, the ry of light at both the excitatory wavelength and the on wavelength to the same first region 206 enables both light W0 2018/140984 PCT/U82018/016053 pulses to share at least a portion of the l path traveled through the s of the patient 202 n the point of entry at the first region 206 and the point of detection at the second region 208 and the third region 210. As discussed in detail herein below, this arrangement of optical paths enhances the y of data produced by the system 200.

In one aspect, the first light source 218 and the second light source 220 may be operatively coupled to a common means of light delivery. In one aspect (not illustrated) the first light source 218 and the second light source 220 may each be operatively coupled to a first optic fiber and a second optic fiber, respectively, and the first and second optic fibers may be joined to a third optic fiber configured to direct light from the first optic fiber and/or the second optic fiber into the first region 206 of the patient 202. In r aspect, the first light source 218 and the second light source 220 may be operatively coupled to a common optic fiber or other optical assembly configured to direct the light from the first light source 218 and/or the second light source 220 into the first region 206 of the patient 202. In this aspect, the light produced by the first light source 218 and the second light source 220 may be directed in an alternating pattern into the common optic fiber or other l assembly using an adjustable optical device including, but not limited to, dichroic mirror or a rotating mirror.

In an aspect, the system 200 may include the sensor head 204 provided with a sensor mount 912 configured with one or more wells within which the light sources 218/220 and light detectors 222/224 may be attached in a predetermined arrangement. In one aspect, illustrated in and , the first light source 218 and the second light source 220 may be situated within a source well 902 of the sensor mount 912 positioned within the sensor head 204 (see . In an aspect, the source well 902 may contain a first LED light source 218 producing light at the excitation wavelength and a second LED light source 220 ing light at the on ngth operatively coupled to a single light delivery aperture 1002 (see ) formed through the aperture plate 702, which ensures that both wavelengths of light (i.e. tory and emission) enter the skin of the patient 202 at approximately the same location including, but not limited to, a first region 206 as illustrated schematically in In an aspect, the source well 902 further contains a first monitor photodiode 904 and a second monitor photodiode 906, which are used to correct for variations in output power from the LED light sources as described in further detail herein below.

W0 2018/140984 PCT/U82018/016053 In an aspect, only a fraction of the light energy produced by the LED light sources is red to the skin of the patient 202 via the single light delivery aperture 1002. In one aspect, the skin of the t 202 receives about 1% of the light energy produced by the LED light sources. In various other aspects, the skin of the patient 202 receives about 2%, about 3%, about 4%, about 5%, about 7.5%, about 10%, about 20%, and about 50% of the light energy produced by the LED light sources. Without being limited to any particular theory, the fraction of light produced by the LED light sources delivered to the skin of the patient 202 may be increased by the incorporation of additional optical elements red to focus and/or direct the light from each LED light source to the light delivery aperture 1002. In r aspect, a diffuser may be used to mix the output of the light s so that the light energy is rendered homogeneous at the surface of the skin of the patient. it) Light detectors Referring again to the system 200 further includes a first light detector 222 and a second light detector 224 in various s. In an aspect, the first light or 222 is configured to measure unfiltered light emitted from the tissue of the patient 202 at the second region 208, and the second light detector 224 is configured to measure filtered light emitted from the tissue of the patient 202 at the third region 210. In this aspect, the second light detector 224 further comprises a optical filter 244 configured to block light at the excitation wavelength. As a result, the first light detector 222 is configured to measure light received at both the excitation and emission wavelengths and the second light detector 224 is configured to detect light received at the emission ngth only. Combined with the illumination of the tissues of the patient 202 with light at the excitatory wavelength only and at the emission wavelength only in an alternating series (see the measurements from the first light detector 222 and a second light or 224 may be analyzed as described herein below to measure the cence of an exogenous fluorescence agent and to correct the fluorescence measurements by ng the effects of dynamic changes in the background signal to the correction methods described herein below.

W0 2018/140984 In various aspects, the second region 208 and third region 210 within the tissues of the t 202, from which light is detected by the first light detector 222 and a second light detector 224, respectively, are each separated by a nominal distance from the first region 206 to which light produced by the first light source 218 and the second light source 220 is delivered. This nominal separation distance may be selected to balance two or more effects that may impact the quality of data detected by the light detectors. Without being limited to any particular theory, as the nominal separation distance increases, the total detected signal from the light detectors may decrease due to light scattering along the longer optical path between light source and light detector. This effect may be ted by the choice of emission wavelength, which may result in a less pronounced se in the detected fluorescence signal (i.e. light at the emission wavelength) relative to the signals associated with detected light at the excitation wavelengths as the nominal separation distance increases. Longer nominal separation distances result in higher sensitivity to signal changes due to changing tissue optical properties.

In one aspect, the nominal separation distance may range from 0 mm (i.e. colocation of light sources and light detectors) to about 10 mm. In various other aspects, the nominal separation distance may range from about 1 mm to about 8 mm, from about 2 mm to about 6 mm, and from about 3 mm to about 5 mm. In various additional aspects, the nominal separation distance may be 0 mm, about 1 mm, about 2 mm, about 3 mm, about 4 mm, about 5 mm, about 6 mm, about 8 mm, and about 10 mm. In one , the nominal separation distance may be about 4 mm to balance these competing effects of logarithmic ff of signal and reduced size of the ound signal relative to the signal from the exogenous fluorescent agent. ing again to the first light detector 222 may be positioned within a first detection well 908 of the sensor mount 912 and the second light detector 224 may be positioned within a second detection well 910 of the sensor mount 912 within the sensor head 204. The first light detector 222 and the second light detector 224 may receive light from tissue of the patient 202 through a first detector aperture 1004 and second detector re 1006, respectively. In an , the first detector aperture 1004, the second detector re 1006, and the light delivery aperture 1002 are mutually separated from one another by the l separation distance disclosed herein above including, but not limited to, a nominal separation distance of 4 mm. In an , the first detection well W0 2018/140984 908, second detection well 910, and light source well 902 of the sensor mount 912 may be optically isolated from one another to ensure that light from the light s 218/220 does not reach the light detectors 222/224 without coupling through the skin of the t 202.

The separation between the two detection wells 908/910 s that the detected cence signal from the exogenous fluorescent agent is distinguishable from the unfiltered excitation light, as described in detail herein below.

In an aspect, the three apertures 704 of the aperture plate 702 (see are circular with a diameter ranging from about 0.5 mm to about 5 mm. In various other aspects, the diameters of the apertures may range from about 0.5 mm to about 1.5 mm, about 1 mm to about 2 mm, about 1.5 mm to about 2.5 mm, about 2 mm to about 3 mm, about 2.5 mm to about 3.5 mm, about 3 mm to about 4 mm, about 3.5 mm to about 4.5 mm, and about 4 mm to about 5 mm.

In one aspect, the three apertures 704 of the aperture plate 702 are circular apertures with a er of about 1 mm diameter. This finite width of the apertures may result in an effective source-detector separation of less than the l separation distance because of the logarithmic drop-off of signal with increasing separation distance from the light sources at the skin interface of the sensor head 204.

In various aspects, the light detectors 222/224 of the system 200 may be any suitable light detection device without limitation. Non-limiting examples of suitable light detection devices e: photoemission detectors such as photomultiplier tubes, phototubes, and microchannel plate detectors, photoelectric detectors such as LEDs reverse-biased to act as photodiodes, photoresistors, photodiodes, phototransistors, and any other suitable light detection devices. In an aspect, the light ors 222/224 are sufficiently sensitive to detect the fluorescence emitted by the exogenous fluorescent agents within the tissues of ts 202 that include melanin ranging from about 1% to about 40% n in the epidermis and blood volume ranging from about 0.5% to about 2% of the skin volume. In one aspect, the light detectors 222/224 may be silicon photomultiplier (SPM) s.

In an aspect, the first light detector 222 may be configured to detect light at both the excitatory frequency and at the emission frequency, and the second light detector 224 may be configured to detect light at the emission frequency only. In one aspect, the W0 2018/140984 PCT/U82018/016053 second light detector 224 may respond only to light of the emission wavelength as a result of the design and materials of the sensor elements of the second light detector 224. In another aspect, the second light detector 224 may respond to a wider range of light ngths, but may be positioned ream from an optical filter configured to pass only the portion of incoming light with the emission wavelength and further configured to block the e of light wavelengths e of the emission wavelength.

Any suitable optical filter may be selected for use with the second light detector 224 to detect light ively at the emission wavelength. Non-limiting examples of suitable optical filters include absorptive filters and interference/dichroic filters.

Without being limited to any particular theory, the performance of an absorption filter does not vary significantly with the angle of incident light, whereas the performance of an interference/dichroic filter is sensitive to the angle of incident light and may require additional collimation optics to effectively filter the Lambertian light bution representative of light emitted from the skin of the patient 202.

In one aspect, the second light detector 224 may be positioned downstream of an absorptive long-pass filter configured to pass light above a predetermined wavelength to the second light detector 224. By way of non-limiting example, the second light or 224 may be positioned downstream of an long-pass OG530 filter configured to pass light with wavelengths above about 530 nm. Other non-limiting examples of suitable filters include a Hoya 054 filter and a Hoya CM500 filter.

In s aspects, an l filter 244 configured to absorb excitation wavelength light may be positioned within the second detection well 910 between the second light detector 224 and the second detector aperture 1006. In one aspect, the optical filter 244 may be constructed from OG530 Schott glass. The thickness of the l filter 244 may be selected to enable an optical density ent to filter the excitation light by about three orders of magnitude. In one aspect, the thickness of the optical filter 244 may range from about 1 mm to about 10 mm. In various other aspects, the ess of the optical filter 244 may range from about 1 mm to about 8 mm, from about 2 mm to about 6 mm, and from about 3 mm to about 5 mm. In various additional aspects, the thickness of the optical filter 244 may be about 1 mm, about 2 mm, about 3 mm, about 4 mm, about 5 W0 2018/140984 mm, about 6 mm, about 7 mm, about 8 mm, about 9 mm, and about 10 mm. In one aspect, the optical filter 244 is a 3-mm thick filter constructed of OG530 Schott glass.

In an additional aspect, an optical diffuser may be provided within the light source well 902. In this aspect, the optical diffuser enables mixing of the light entering the light source well 902 from the first and second light sources 218/220. By mixing the light from the first and second light sources 218/220 using the optical diffuser prior to illumination of the first region 206 of the patient 202, the similarity of the optical paths taken by emission-wavelength light and tion-wavelength light through the tissues of the patient is enhanced relative to the corresponding l paths taken by unmixed light, thereby reducing a potential source of variation.

In an aspect, a arent material configured to pass light of both the excitatory and emission wavelengths may be positioned within the first detection well 908 between the first light detector 222 and the first detector aperture 1004. In this , the transparent al may be any material with similar l properties to the material of the l filter 244 including, but not limited to, thickness and index of refraction. In one aspect, the arent al within the first detection well 908 may be fused silica glass of the same thickness as the optical filter 244.

By way of non-limiting example, the transmission spectrum of the 0G 530 filter is provided in As illustrated in the transmission spectrum of the 0G 530 filter overlaps with the emission spectrum of the 1Vfl3-102 exogenous fluorescent agent and the emission spectrum of a green LED used as a second light source 220 (emission wavelength). In addition, the transmission spectrum of the 0G 530 filter excludes the emission spectrum of the blue LED used as a first light source 218 and the absorbance spectrum of the MB-102 exogenous fluorescent agent (excitation wavelength).

In an aspect, the transparent material such as glass 246 and the optical filter 244 may be secured to ledges formed within the first detection well 908 and the second detection well 910, respectively. The transparent material such as glass 246 and the optical filter 244 may be secured in place using an opaque and/or light absorbing adhesive including, but not limited to, black epoxy to ensure that all light received through the first detector aperture 1004 and the second detector aperture 1006 travels through the optical filter 244 or glass 246 before detection by the first and second light ors 222/224. In W0 2018/140984 another aspect, the sides of the optical filter 244 or glass 246 may be painted black with a light-absorbing coating including, but not limited to, India ink to ensure that light does not reach the first and second light detectors 222/224 without passing through the optical filter 244 or glass 246.

In an aspect, the height of the detection wells 908/910, combined with the er of the detector apertures 1004/1006 may limit the fraction of the light emitted from the second region 208 and third region 210 of the patient’s skin that reaches the active areas of the light detectors 222/224 due to the Lambertian distribution of the angle of the light leaving the patient’s skin. In one aspect, the on of light emitted from the second region 208 and third region 210 of the patient’s skin received by the light detectors 222/224 may range from about 5% to about 90%. In various other aspects, the fraction of light may range from about 5% to about 15%, from about 10% to about 20%, from about 15% to about 25%, from about 20% to about 30%, from about 25% to about 35%, from about 30% to about 40%, from about 35% to about 45%, from about 40% to about 60%, from about 50% to about 70%, and from about 60% to about 90%.

In one aspect, for the sensor head 204 rated in and with 1-mm diameter apertures 1002/1004/1006, about 10% of the light emitted from the surface of the patient’s skin may reach the active area of the light ors 222/224 to be detected.

In various aspects, the sensor head 204 may r include additional l elements including, but not limited to, lenses and/or prisms configured to compensate for the Lambertian distribution of light angles in order to enhance the on of light emitted from the patient’s skin that is directed to the active area of the light detectors 4. iii) Temperature sensors Referring to the sensor head 204 may r include one or more additional temperature sensors 228 configured to monitor temperatures of various regions within the sensor head 204 and in the vicinity of the sensor head 204. Non-limiting examples of suitable s for which the temperature may be monitored by the one or more additional temperature sensors 228 include: temperature at the skin surface of the patient 202, temperature in the vicinity of the first light source 218 and/or second light source 220, ambient temperature outside of the sensor head 204, temperature of housing 600 of sensor head 204, and any other suitable region. In one aspect, additional W0 2018/140984 temperature sensors 228 may be configured to r the temperatures in the vicinity of temperature-sensitive electrical components including, but not limited to: light sources 218/220 such as LEDs, light detectors 222/224 such as silicon photomultipliers (SPMs), and any other temperature-sensitive electrical components of the sensor head 204. In some aspects, one or more temperatures measured by one or more additional temperature sensors 228 may be used as feedbacks in a control method for one or more of the temperature- sensitive s of the system 200 as described herein below.

By way of non-limiting example, a temperature measurement may be used to control the amount of light energy produced by an LED used as a first or second light source 218/220. In this example, LED temperatures measured by an second temperature sensor 1108 (see ) may be used in a control scheme to modulate the amount of power ed to an LED light source to compensate for the effect of LED temperature on the light output of the LED. In another aspect, additional ature sensors 228 may monitor the temperatures of LED light sources 218/220 to monitor and/or compensate for temperature variations of the LEDs as well as to monitor and/or sate for temperature-dependent transmission of the optical filters to maintain relatively constant output wavelengths.

By way of another miting example, an additional ature sensor 228 may be included in the sensor head 204 in the form of a temperature sensor 816 (see configured to monitor the temperature of the housing 600 in the vicinity of the t surface 606 of the sensor head 204. ing to FIG, 8, and the ature sensor 816 may be epoxied into the temperature sensor opening 706 in the aperture plate 702 in one aspect. In this aspect, the space 918 between the circuit board (not shown) and the lower housing 604 may be filled with a thermally conductive putty to ensure good thermal conduction and dissipation.

In this example, the measured housing ature may be used to modulate the light output of the sensor head 204 to prevent overheating of the skin of the patient 202 during use. In r aspect, additional temperature sensors 228 may monitor the temperatures of LED light sources 218/220 to r and/or compensate for temperature variations of the LEDs to enable the maintenance of relatively constant output wavelengths by the LED light sources 218/220.

W0 2018/140984 -3 5- 2018/016053 In an additional aspect, temperatures measured by one or more additional temperature sensors 228 may provide for subject safety by disabling one or more electrical devices ing the light sources 218/220 and/or light ors 4 if an over- temperature condition is detected. In one aspect, an over-temperature condition may be indicated if the housing temperature detected by the temperature sensor 816 is greater than about 40°C. In various other aspects, an over-temperature condition may be detected of the housing ature is greater than about 405°C or greater than about 41 .00 C.

B. Controller Referring again to the system 200 in s aspects may e a controller 212 configured to operate the light sources 218/200 and light detectors 222/224 in a coordinated fashion to obtain a plurality of measurements used to obtain the fluorescence of the exogenous fluorescent agent within the tissues of the patient 202, to correct the fluorescence data to remove the effects of dynamic changes in the background signal as described herein below, and to transform the fluorescence ements into a parameter representative of the renal function of the patient 202. is a schematic diagram of an electronic circuit 1100 that rates the arrangement of various electrical ents that enable the operation of the system 200 in an aspect. In one aspect, the ller 212 may be a computing device further including an operation unit 214 and a y unit 216. 1) Light source control unit Referring again to the controller 212 may include a light source control unit 230 configured to operate the first light source 218 and the second light source 220 to produce light at the excitation wavelength and emission wavelength, respectively in a coordinated manner to produce a repeating pulse sequence as illustrated schematically in In various aspects, the light source control unit 230 may produce a plurality of light control signals encoding one or more light control ters including, but not limited to: activation or deactivation of each light , relative timing of activation and deactivation of each light source to enable light pulse width, pulse repetition rate, electrical power delivered to the light source or other parameter associated with light pulse fluence or light pulse power, other light source-speciflc parameters controlling the light output of the light source, and any other relevant light control parameter. In an aspect, the light source W0 2018/140984 l unit 230 may receive one or more feedback measurements used to modulate the ity of control signals to compensate for variations in performance of the light sources in order to maintain a relatively stable output of light from the light sources. Non-limiting examples of feedback measurements used by the light source control unit 230 include: light output of the light sources 0 measured within the source well 902 by the first monitor photodiode 904 and the second monitor photodiode 906, respectively, temperatures of the light sources 218/220, and any other feedback measurement relevant to monitoring the mance of light sources 218/220.

By way of non-limiting example, the light source control unit 230 may be configured to e LED light sources 218/220. In this e, the light output of the LED light sources 218/220 may be controlled by lling the magnitude of current provided to each LED. In an aspect, the light source control unit 230 may include at least one waveform generator 1122 including, but not limited to, a field programmable gate array FPGA with a 16-bit DAC 1124 operatively coupled to a LED current source 1126, as illustrated in . In an , waveforms generated by the at least one waveform generator 1122 including, but not limited to square waves, may control the output from the LED current source 1126. In an aspect, the magnitude of the current supplied to the LED light sources 218/220 may be adjustable based on the rm signals ed by the waveform generator/FPGA 1122.

Referring to in one , each light pulse sequence 500 includes an emission wavelength light pulse 502 and an excitatory wavelength light pulse 504 that are both made up of a plurality of square waves 506 produced by the first and second LED light sources 218/220. Referring to , square waves generated by the waveform generator 1122 are ed by the LED current source 1126. The current generated by the LED current source includes a square waveform similar to the waveform generated by the waveform generator 1122. Without being limited to any particular theory, because the intensity of light produced by the LED light sources 218/220 is proportional to the magnitude of the current received, the light ed by the LED light s 218/220 also includes the square waveform as illustrated in In another aspect, discussed in additional detail below, the square waves produced by the rm generator 1122 may also be used by the acquisition unit 234 in a synchronous detection method to reduce the effects of various confounding factors including, but not limited to, the detection of W0 2018/140984 ambient light, from the detector signals generated by the light detectors 222/224 during illumination of the tissues of the patient at the emission and tory wavelengths by the first and second light sources 218/220, respectively.

In various other aspects, a variety of alternate LED pulse modulation schemes may be equivalently employed without limitation. In one aspect, the excitation and emission pulses are delivered in an alternating series persed with a dark period after each pulse. In another aspect, the first and second LED light sources 218/220 are each modulated with a 50% duty cycle but at different tion frequencies, allowing the signals associated with the excitation and emission pulses to be separated by frequency filtering.

Without being limited to any particular theory, the overall optical power delivered to the patient’s skin may be d by at least two factors: photobleaching of the exogenous fluorescent agent and/or endogenous phores, as well as overheating of the patient’s tissues nated by the system 200. In one , tissue heating may impose an absolute limit of about 9 mW on the optical power that can be delivered to the skin, based on safety standards including, but not limited to, ANSI/IESNA RP-27.1-05. In another , leaching of the skin autofiuorescence associated with endogenous chromophores including, but not d to, collagen, obin, and melanin may contribute a background signal to the measured fluorescence that remains vely constant so long as no eaching of the chromophores occurs. This constant autofiuorescence background may be subtracted from the raw fluorescence signal, but if autofiuorescence varies over time due to photobleaching, this background correction may interfere with the c calculation of the renal decay time constant (RDTC). In an aspect, the light output power of the first light source 218 and/or second light source 220 may be limited to levels below power thresholds associated with chromophore photobleaching.

Referring again to the light output of the light sources 218/220 may be measured using monitor photodiodes 904/906 in various aspects. Because the light ity reaching these monitor photodiodes 904/906 is typically much stronger than the light intensity that reaches the light detectors 222/224 through the patient’s skin, less sensitive light detecting devices including, but not limited to, PIN photodiodes may be used to monitor the output of the light sources 218/220.

W0 2018/140984 -3 8- PCT/U82018/016053 In various aspects, the system 200 may be configured to operate over a range of skin tones observed in the human population. Without being limited to any particular theory, variations in skin tones between ent patients 202 may result in ions in the detected fluorescence signals ranging over about three orders of ude. In addition, variations in the concentrations of exogenous fluorescent agent within each patient 202 may vary over a range of about two orders of magnitude due to renal elimination of the agent over time. In various aspects, the system 200 may be configured to detect cence from the endogenous fluorescent agent over an intensity range of more than five orders of magnitude. In these various aspects, the system 200 may be configured by modulation of at least one operational parameter including, but not limited to: magnitude of light output by the light sources 218/220 and sensitivity of light detectors 222/224 ponding to detector gains.

In one aspect, the intensity of the light output by the light sources 218/220 may be manually set by a user via the operation unit 214. In another aspect, the light source control unit 230 may be configured to te the intensity of light produced by the light sources 218/220 automatically. In an aspect, the light source control unit 230 may be configured to control the light intensity produced by the LED light sources 218/220 within a range of normalized output intensities from 0 (off) to 1 (maximum . In an , the ity of the light sources 218/220 may be set by the light source control unit 230 in coordination with the detector gains of the light detectors 222/224 set by the light detector control unit 232, as described herein below.

In one aspect, signals ed during the first 10 detection cycles ed by the system 200 after initialization of data ition, but prior to the injection of the exogenous fluorescent agent, may be used by the light source l unit 230 to automatically adjust the light intensity produced by the LED light sources 218/220, as well as the gain of the light detectors 222/224. In this example, the initial detection cycle may be obtained with the LED light sources 218/220 set at about 10% of maximum LED intensity (corresponding to a normalized output intensity of 0.1) and with a low gain setting for the light detectors 222/224. Based on the detected intensity of light received at the light detectors 222/224 at the excitation and emission wavelengths for one ion cycle, the corresponding LED intensities may be modulated to enable the analog signals produced by the light detectors 222/224 to correspond to about 1A of the full range of each detector W0 2018/140984 PCT/U82018/016053 analog-to-digital convertor (ADC) at the low detector gain setting. If the signals produced by the light detectors 222/224 in se to the light produced by the second LED light source 220 at the emission wavelength do not agree, the larger signal may be used to modulate the power setting of the second LED light source 220. If the method described above results in modulation to an LED intensity g higher than the maximum intensity (corresponding to a normalized output intensity of 0.1), the LED intensity setting is set to the maXimum setting. t being limited to any particular theory, the targeted levels of signals produced by the light detectors 222/224 (i.e. 1A of the ADC range) is selected to reserve additional light detection capacity to detect signals resulting from variations in optical properties of the tissues of the patient 202 during the study due to any one or more of a plurality of factors including, but not limited to, the introduction of the exogenous fluorescent agent into the patient 202.

In the above one aspect, once the LED intensities are set by the light source l unit 230 in coordination with the detector gains of the light detectors 222/224 set by the light detector l unit 232 over the first 10 ion cycles, an additional 10 ion cycles are obtained to confirm the suitability of these settings for operation of the system 200 given the tissue properties of the particular patient 202, followed by a recalculation of the LED intensity settings and detector gains as described . If the newly calculated LED intensity is within a factor of two of the previously determined setting, and the detector gains are not d, the previously determined settings are maintained for subsequent data ition cycles used to determine renal function.

Otherwise, the settings are updated using the same method described herein and another 10 data acquisition cycles conducted to confirm the ity of the settings. This process repeats until either the settings are determined to be acceptably stable or 10 data acquisition cycles are conducted to obtain the settings, in which case the most recently determined settings are used for all subsequent data acquisitions, and the user may be notified via the display unit 216 that the gs may not be optimal. it) Light detector control unit Referring again to the controller 212 may include a light detector control unit 232 configured to operate the first light detector 222 and the second light or 224 to enable the detection of light at the emission wavelength and unfiltered light W0 2018/140984 PCT/U82018/016053 at all wavelengths, respectively. In various s, the light detector control unit 232 may produce a plurality of detector control signals encoding one or more detector control parameters including, but not limited to, detector gains. In various other aspects, the light detector control unit 232 may produce a plurality of light measurement signals encoding the ity of light detected by the light detectors 222/224 including, but not limited to raw detector signals that may be received by an analog-to-digital convertor (ADC) 1102 (see ) in various aspects. In another aspect, the detector gains and/or other detector control signals may be manually set by a user or gains when the system 200 is configured in an Engineering Mode.

In various other aspects, the amount of light received by the light detectors 222/224 may vary due to any one or more of at least l s including, but not limited to: variation in skin tones observed between individual patients 202, variations in the concentrations of exogenous fluorescent agent within each patient 202, and any other relevant parameter. In one aspect, gains of the first light detector 222 and the second light detector 224 may be set by a user via the operation unit 214. In r aspect, the light detector l unit 232 may be configured to modulate the gain of the light detectors 222/224 automatically via a bias voltage gain of the bias voltage generator 1112 (see ).

In one aspect, signals obtained during the first 10 detection cycles ed by the system 200 after initialization of data acquisition, but prior to the injection of the exogenous fluorescent agent, may be used by the light detector control unit 232 to automatically adjust the gains of the light detectors 222/224, as well as the output intensities of the light s 218/220. As described herein previously, the l detection cycle may be obtained with the LED light sources 0 set at about 10% of maximum LED ity sponding to a normalized output intensity of 0.1) and with a low gain setting for the light detectors 222/224 and the LED intensities may be modulated to enable the analog signals produced by the light detectors 222/224 to correspond to about 1A: of the full range of each detector analog-to-digital convertor (ADC) at the low detector gain setting.

W0 2018/140984 2018/016053 In this one aspect, if the intensity of the first LED light source 218 cing light at the excitation ngth) is set to the maximum of the LED power range, a high detector gain may be considered for the second light detector 224 corresponding to the filtered measurements of the excitation wavelength only. In various aspects, the high detector gain may be 10-fold higher than the corresponding low detector gain for a given light detector. Without being limited to any particular theory, the expected peak ed fluorescence signal from the exogenous fluorescence agent over the course of injection and renal ation is typically expected to be about 10% of the magnitude of the signal received during illumination at the excitation wavelength by the first light source 218, assuming that the exogenous fluorescence agent is MB-102 introduced into the patient 202 at a dose level of about 4 umol/kg of patient weight. In an aspect, if the expected detector signal received during illumination at maximum LED intensity and with the detector gain set to the high setting remains below 10% of the range of the detector ADC, the detector gain for that measurement be increased by ten-fold. In another aspect, the saturation ion may persist for a pre-defined period of time ing, but not limited to, a 30-second period before adjustments are made to the detector gain or LED power to avoid reacting to spurious signal spikes.

In another , the light detector control unit 232 may adjust the detector gain to a lower gain level if the ed light signals from one of the light detectors 4 exceed a threshold percentage of the maximum ADC range to avoid signal saturation. Although the highest threshold percentage of the maximum ADC range associated with signal tion is 100%, the onset of severe detector non-linearity takes place at threshold percentages of about 40% or more, and mild detector non-linearity occurs at threshold percentages in excess of about 15%. In various aspects, the threshold percentage of the maximum ADC range may be 40%, 35%, 30%, 25%, 20%, 18%, 17%, 16%, 15%, 14%, 13%, 12%, 11%, 10%, 9%, 8%, 7%, 6%, or 5% of the maximum ADC range. In one aspect, if the ed light signals from one of the light detectors 222/224 exceed about 8% of the maximum ADC range, the gain setting will be adjusted. By way of non-limiting example, if the or gain on the nearly saturated signal is high, it will be adjusted to low. If the current detector gain is set to low and the corresponding detected light signal remains above the threshold percentage of the maximum ADC range, the LED output power setting of the corresponding LED light source may be reduced ten-fold.

W0 2018/140984 2018/016053 In an aspect, the light or control unit 232 may receive one or more feedback measurements used to modulate the ity of detector signals to sate for variations in the mance of the light detectors due to variations in temperature and/or light source output. Non-limiting examples of feedback measurements used by the light detector control unit 232 include: light output of the light sources 218/220 measured within the source well 902 by the first monitor photodiode 904 and the second monitor photodiode 906, respectively (see ), temperatures of the light detectors 222/224 measured by a first temperature sensor 1106, LED temperatures measured by a second temperature sensor 1108, temperature of the sensor head housing measured by a third temperature sensor 1128, LED supply current from the LED current source 1126, and any other feedback measurement nt to monitoring the performance of light detectors 222/224.

In various aspects, the light detectors 222/224 may be n photon multiplier (SPM) detectors that may e low-noise internal amplification, and may function at lower light levels relative to other light sensor devices such as PIN photodiodes.

The detector signal generated by the SPM detectors 222/224 may be amplified using mpedance amplif1ers 1120/1118, respectively (see ) to ate a t generated by each SPM light detector 222/224 into a measurable detector voltage. The transimpedance amplif1er 1118 on the second SPM light detector 224 (i.e. detects filtered lights at the excitation wavelength only) may include a switchable detector gain that may select a low gain configured to detect a larger dynamic range for fluorescence measurements when the first LED light source 218 is activated to e light at the emission wavelength. The switchable detector gain that may further select a high gain setting for the second SPM light detector 224 when the second light source 220 is inactive to enhance the sensitivity of the second SPM light detector 224 during the phase of the detection cycle when light at the emission wavelength produced by the exogenous fluorescent agent within the tissues of the patient 202 is detected, to ensure that the expected dark current from the second SPM light detector 224 es less than 1A: of the total ADC output range. In one aspect, the second transimpedance amplifier of the second SPM light detector 224 may include a low detector gain configured to provide a transimpedance gain of about 4 k9 ponding to about twice the value of the transimpedance resistor due to differential operation, and may further include a high W0 2018/140984 detector gain configured to e a transimpedance gain of about 40 k9. In another aspect, the first mpedance amplifier of the first SPM light detector 222 may include a fixed detector gain configured to provide a transimpedance gain of about 2 k9. iii) Acquisition Unit Referring again to the controller 212 may further include an acquisition unit 234 in various aspects. The acquisition unit 234 may be configured to receive a plurality of signals from the light sources 218/220, light detectors 222/224, and additional light detectors 226 and onal temperature sensors 228 and processing the plurality of s to produce one or more raw signals including, but not limited to, raw fluorescence signals encoding the intensity of fluorescence detected by the second light or 224 during nation at the excitation wavelength, and raw internal reflectance signals corresponding to the intensity of light at the excitation wavelength ed by the first light detector 222 during illumination at the excitation wavelength as well as the intensity of light at the emission wavelength detected by the both light detectors 222/224 during illumination at the emission wavelength.

The plurality of signals received from the various sensors and s bed herein above are typically analog signals including, but not limited to, electrical voltages and currents. In various aspects, the acquisition unit 234 may enable the transmission of the analog signals to one or more analog-to-digital converters (ADCs) to convert the analog signals into digital signals for subsequent processing by the processing unit 236. is a schematic diagram of a circuit 1100 illustrating the arrangement of various electrical devices and components of the sensor head 204. In one aspect, the analog signals encoding the intensity of light detected by the first light detector 222 and the second light detector 224 may be received by a first ADC 1102.

In various aspects, the analog signals produced by the light detectors 222/224 and various monitor s may be digitized using at least one 24-bit Sigma- Delta ADC. Referring again to , analog s encoding the measurements from time-sensitive sensors may be digitized using a high-speed 24-bit Delta ADC 1102 in one aspect. In this aspect, time-sensitive sensors include sensors associated with the production and detection of light pulses characterized by potentially y-changing signals. Non-limiting examples of time-sensitive sensors of the system 200 include: first W0 40984 and second light detectors 1118/1120, and first and second monitor photodiodes 904/906.

In another aspect, analog signals encoding the ements from less time-sensitive sensors may be digitized using a low-speed 24-bit Sigma-Delta ADC 1104. In this other aspect, the less time-sensitive sensors include sensors associated with monitoring system conditions characterized by lly slow-changing signals including, but not d to, temperatures of various system ents and/or regions. Non-limiting examples of less time-sensitive sensors of the system 200 include: a first and second temperature sensor 108 configured to monitor the temperatures of the light detectors 222/224 and light sources 218/220, respectively, and a third temperature sensor 1128 configured to monitor a temperature of the housing 600 of the sensor head 204.

In various aspects, the ition unit 234 may be further configured to enable synchronous detection of light by detectors 222/224. Without being limited to any particular theory, synchronous detection methods are t to reject noise from the or signals associated with the detection of light produced by the light sources 118/120 and fluorescence produced by the exogenous fiuorescent agents within the tissues of the patient 202 by distinguishing the detector signals from noise associated with the detection of ambient light or other sources of interference. is a schematic illustration of a synchronous detection method in one aspect. Referring to and , the waveform generator/FPA 1122 may generate a digital square wave 1202 that is received by the DAC 1124, and the resulting analog- converted square wave is received by the LED current source 1126. The ing current produced by the LED current source 1126, also terized by a waveform proportional to the analog-converted square wave drives LED light s 0. The light produced by LED light sources 218/220, after passing through the tissues of the patient 202 are detected, along with the fluorescence produced by the endogenous fiuorescent agent, by the light detectors 222/224 and are digitized by the high-speed ADC 1102.

Referring again to and , the digital square wave 1202 generated by the waveform generator/FPA 1122 may also be converted by a DAC 1110 (see ) to an in-phase reference sine wave 1210 and an out-of-phase/quadrature reference cosine wave 1212. In an aspect, the digitized detector signals from the ADC 1102 and the in-phase reference sine wave 1210 may be sampled and subjected to signed W0 2018/140984 multiplication at a first multiplier 1214 to generate a plurality of in-phase modulated signals. In addition, the digitized detector signals and the quadrature reference cosine wave 1212 may be sampled and subjected to signed multiplication at a second multiplier 1216 to generate a plurality of quadrature (out-of-phase) modulated signals. In this aspect, the acquisition unit 234 may delay the samples from the reference waves 1210/1214 by an amount equivalent to the relative delay between the DAC 1124 generating the reference waves 1210/1214 and the ADC 1102 digitizing the detector signals to synchronize the reference waves 1210/1214 to the detector data being acquired.

Referring again to , the in-phase modulated signals may be summed in a first accumulator 1218 to generate an se intensity signal 1224. Similarly, the quadrature modulated signals may be summed in a third lator 1222 to generate a quadrature intensity signal 1228. The raw digitized detector signal may also be summed in a second lator 1220 to generate an average intensity signal 1226. In on, the in-phase intensity signal 1224 and the quadrature intensity signal 1228 may be root-sum squared to generate a magnitude signal 1230. t being limited to any particular theory, the integration interval of the accumulators 1218/1220/1222 may correspond to an integer number of modulation cycles sponding to cycles of the l square wave 1202) to avoid a bias on the measured signal. The phase accumulators 220/1222 used to control the synchronous detection operates on integer numbers, but the sample clock frequency and the modulation frequency are not integer-divisible, so the number of cycles is not exactly an integer. However, the error associated with this mismatch may be minimized by adjusting the actual modulation ncy to match as y as possible with the achievable sampling als and allocating an appropriate number of bits to the phase accumulator. In one aspect, the error associated with the mismatch between the modulation frequency and the sampling intervals may be on the order of about one part in 106.

In one aspect, the digital square wave 1202 used to modulate the LED light sources 218/220 and to enable onous detection method as described herein above is ed at a frequency of about 1 kHz. t being limited to any particular theory, a square wave was selected as the modulating waveform to enable an enhancement in signal W0 2018/140984 ZOlS/016053 to noise ratio (SNR), as compared to a pure sinusoidal wave as the modulating waveform for the same peak power level.

In another aspect, the acquisition unit 234 may be further configured to enable demodulation of the in-phase intensity signal 1224, average ity signal 1226, and quadrature intensity signal 1228. In one aspect, the acquisition unit 234 may pick out each component at the fundamental harmonic, which is characterized by an amplitude that is (4/71) times larger than the amplitude of the square wave 1202 used to te the intensity signals 1224/1226/1228. In various aspects, to reject 50/60 Hz electrical noise generated by the alternating current electrical power sources, and corresponding 100/120 Hz optical noise generated by ambient light sources powered from those electrical power sources, the integration period of the accumulators 1218/1220/1222 may be selected to be a multiple of 100 ms. In these various aspects, this selected integration period ensures that integration by the accumulators 1218/1220/1222 occurs over an integer number of cycles for the 50, 60, 100, and 120 Hz signals. iv) sing unit Referring again to the controller 212 may further include a processing unit 236 configured to apply tions to the demodulated detector signals and to transform a ed portion of the ted detector signals into a measure of renal function in various aspects. is a block diagram illustrating the subunits of the processing unit 236 in an aspect. Referring to , the processing unit 236 may include a pre-processing subunit 1302 configured to determine and correct the detector s to remove signal artifacts associated with a variety of confounding effects including, but not d to, physiologically-induced signal variations, variations in power supplied to the light sources 218/220, nearities in detector se, ambient temperature variation, and tissue heterogeneity. The processing unit 236 may further include a background subtraction subunit 1304 configured to remove the portion of the detector signals attributable to background factors such as autofiuorescence of the tissues and/or leakage of light at the excitation wavelength through the optical filter 244 of the second light detector 224. The sing unit 236 may additionally include a background correction t 1306 configured to enable a method of applying a background correction method to remove W0 2018/140984 the effects of dynamic s in the background signal related to changes in autofiuorescence and/or the leak-through of tory-wavelength light to the second light detector 224 configured to detect emission-wavelength light only, and to apply the background correction to the first detector, turning DRemm into DRewhotm. The sing unit 236 may further include a post-agent administration selection subunit 1308 configured to select a portion of the detector data associated with the post- equilibration period for subsequent analysis to ine renal on of the patient.

The processing unit 236 may further include an RDTC calculation subunit 1310 configured to transform the detector signals obtained over the post-equilibration period to e a renal decay time constant indicative of the renal function of the patient.

The processing unit 236 may also include a fault detection subunit 1312 configured to monitor the magnitudes of the detector signals to detect any malfunctions of the system. - pre-processing subunit In one aspect, the raw signals corresponding to the light intensity ed by light detectors 222/224 ponding to illumination by the first light source 218 and the second light source 220 at the tion and emission wavelength, respectively, are pre-processed using various modules of the pre-processing subunit 1302 to remove the effects of a plurality of confounding factors from the raw signals, resulting in signals that more accurately reflect the underlying specific signals of interest.

By way of several non-limiting es, the intensity of light produced by a light source may vary due to one or more of a plurality of factors including, but not limited to: fluctuations in the electrical current supplied to the light source and variations in the ambient temperature of the light source. Light characterized by two or more wavelengths emanating from the same source re of the sensor head may not share the same path to the same detector. The detectors may have thermally-dependent sensitivity and gain. Further, the l filter associated with the second light detector 224 may have ature-dependent transmission properties.

In one aspect, the pre-processing subunit 1302 is configured to process the raw signals corresponding to light intensities detected by the first and second light detectors 4 in order to remove one or more of the measurement errors associated with the devices and elements of the system 200 and patient-specific factors including, but W0 2018/140984 not limited to, the plurality of factors described above. A is a block m illustrating the modules of the pre-processing t 1302 in one aspect. B is a block diagram illustrating the modules of the pre-processing subunit 1302a in a second aspect.

In one aspect, illustrated in A, the pre-processing subunit 1302 1) resamples the signals using the methods of the resampling module 2202 as described below, 2) removes saturated or signals using the methods of the detector output saturation detection and removal module 2204 as described below, 3) corrects for temperature-dependent detector gain using the methods of the detector temperature correction module 2206 described below, 4) corrects the signals for instrument light ionality using the methods of the light directionality correction module 2208 described below, 5) corrects the signals for filter throughput and temperature-dependent variation of fluorescence light using the methods of the filter throughput temperature correction (emission) module 2212 described below, 6) corrects for tissue geneity using the methods of the tissue heterogeneity tion module 2216 described below, 7) corrects the signals for filter throughput and temperature-dependent variation of excitation light and signal decomposition using the methods of the filter throughput temperature correction (excitation) module and signal decomposition module 2214 as described below, 8) corrects for optical power variation using the methods of the fractional photon normalization module 2218 as described below.

In one aspect, rated in B, the ocessing subunit 1302a calculates signal magnitudes using the methods of the detector temperature correction module 2206a as described below, resamples the signals using the methods of the resampling module 2202a as described below, removes saturated samples using the methods of the detector output saturation ion and removal module 2204a as described below, corrects the signals for temperature-dependent or gain using the methods of the or temperature correction module 2206a described below, corrects the signals for optical power variation using the methods of the fractional photon normalization module 2218a as described below, corrects for excitation light leakthrough onto the measured cence signal using the filter hput temperature correction ation) module and signal decomposition module 2214a as described below, and corrects for fluorescence W0 2018/140984 PCT/U82018/016053 light leakthrough onto the measured tion diffuse reflectance signal using the filter throughput temperature correction (emission) module 2212a as described below. - resampling module Referring to A and B the pre-processing subunit 1302/1302a in various aspects includes a resampling module 2202/2202a configured to reduce signal variations associated with physiological processes of the t 202 ing, but not limited to, heartbeat and breathing. Typically, an acquisition sequence is characterized by alternating interval of illumination at the excitation and emission separated by intervals of no illumination (i.e. dark intervals). gh both illumination intervals ation/emission) are time-stamped with the same time-stamp value as described above, the dark interval between the excitation and emission illumination intervals results in a separation interval n the tion and emission illumination intervals. t being limited to any particular theory, if the separation interval ated with an ition sequence is on the order of a separation interval between physiological events, such as heartbeats or respiration, logical noise may be introduced to the s. In various s, this physiological noise may be reduced by resampling the signals associated with the excitation and emission illumination to overlap prior to subsequent processing of the signals.

By way of non-limiting example, a sample sequence may include a 100 ms dark interval, a 100 ms interval of illumination at the excitatory wavelength, a second 100 ms dark interval, and a 100 ms interval of illumination at the emission wavelength. Each sample packet is logged with a single timestamp, and each sample packet is separated by a 400 ms interval. Because physiological signal variations, such as from heartbeats, occur on this same timescale, the 200 ms difference between signal ition associated with the excitatory and emission wavelengths becomes apparent in the signals. This physiological signal noise may be reduced using the pre-processing subunit 1302 by first resampling the signals ated with illumination at the excitatory and emission wavelength illumination to overlap prior to performing any additional signal processing as described below. In this non-limiting example, the signals associated with illumination at the excitatory wavelength may be shifted forward by 100 ms and the signals associated with illumination at the W0 2018/140984 PCT/U82018/016053 emission wavelength may be shifted backwards by 100 ms, resulting in an overlap of the signals.

In various aspects, the resampling module 2202 performs resampling as described above on s detected by both the first and second detectors 222/224. In one aspect, the resampling module 2202 functions as a form of low-pass filter. - detector output saturation detection and removal module Referring again to A and B the pre-processing subunit 1302/1302a in s aspects es a detector output saturation detection and removal module 2204/2204a configured to detect and remove signal values that fall outside the detection range of the light detectors 222/224. In one aspect, the pre-processing t 1302 compares the detected signals to the m ADC signal. If any signal falls within a threshold range of the maXimum ADC signal using the average or peak signal value, the detector output saturation detection and removal module 2204 identifies and removes that value from further processing. - detector temperature correction module Referring again to A and B the pre-processing subunit 302a in various aspects includes a detector temperature correction module 2206/2206a configured to enable a temperature correction to compensate for the thermal sensitivity of the light detectors 222/224. In one aspect, the intrinsic detector gain for a silicon photomultiplier (SPM) device typically used as a light detector is proportional to the difference between the device own voltage and the bias e applied by the bias voltage generator 1112 (see ), ed to herein as an overvoltage. In this aspect, the breakdown voltage varies with ature in a haracterized manner. In one , the temperature tion accounts for both this internal detector gain variation and additionally temperature-related variation in the photon detection efficiency.

In one aspect, the temperature correction may be a scaling correction applied to the detector measurements in which the scaling correction is based on a measured detector temperature. In an aspect, the measured light detector signals may be W0 2018/140984 PCT/U82018/016053 divided by the calculated gain C(Z) to remove the temperature dependency. The scaling correction C(Z) may be ated according to Eqn. (2): C(71) : Cv' Vbias _ Vbreakdown(1 + CT)T—TO Eqn~ (2) In Eqn. (2), the monitor temperature T is obtained from a first temperature sensor 1106 (see ) configured to monitor the temperature of the sensors 4.

The bias e (mes) may be measured by the bias voltage generator 1112. The breakdown voltage (mekdown) and reference temperature (T0) are constants specific to the particular light detector device included in the system 200. By way of non-limiting example, if the light detectors 4 are silicon photomultiplier (SPM) devices, Vbreakdow” may be 24.5 V and T0 may be 21 degrees C. In another aspect, the coefficients CV and CT used in Eqn. (2) may be derived cally based on measurements obtained using a constant phantom over an ambient temperature ranging from about 18 degrees C to about 26 degrees C.

In another aspect, the temperature portion of the gain correction is determined by the Eqns. (3)—(5).

GuseCase — Cv_ T —T ' Vbiasmeaswed _ Vbreakdown(1 + CT) measured 0 Eqn~ (3) Gnominal — Cv_ T - —T ' ominal _ down(1 + CT) nominal 0 Eqn~ (4) GuseCase G _ correctlon — ECln~ (5 ) Gnominal, This gain correction can be applied to each of the signal magnitudes as measured by the first and second light detectors 222/224 as follows: SPMmagnitude 5PMmagnitudecorrected = Eqn. (6) Gcorrection In an aspect, the magnitudes of the ements from each or and monitor photodiode are calculated from the root sum-squares of the in-phase magnitude signals 1230 (I) and quadrature magnitude signals 1232 (Q) according to Eqn. (1): M = I2 + Q2 Eqn. (1) W0 2018/140984 PCT/USZOlS/016053 The signal magnitudes from the light detectors 222/224 calculated using Eqn. (1) are normalized by the monitor photodiode ude for each measurement set corresponding to the measurements obtained during illumination by one of the LED light sources 218/220 at either the tion or emission wavelength. Because both monitor photodiodes 904/906 may positioned in the same source well 902 as both LED light sources 218/220 (see , the average of the two monitor photodiode udes from the corresponding measurement set is used.

In an aspect, the in-phase intensity signal 1224, quadrature intensity signal 1228, and average intensity signal 1226 (see ) are further processed for the number of accumulated samples and ADC scaling such that the ity signals 1224/1226/1228 are ed as fraction of the full range of the peed ADC 1102 (i.e. ranging from a minimum of 0 to a maximum of 1). The measurements of the monitor photodiodes 904/906 (see ) are similarly scaled as a fraction of the full range of the low-speed ADC 1104.

In one aspect, Gcorrection may incorporate a power correction to t for the effects of fluctuations in the LED power supply. In this aspect, the signals from the first monitor photodiode 904 and the second monitor photodiode 906 are calibrated by ing optical output power with a power meter as light intensities from the light sources 218/220 are varied. The calibration coefficients for each light source 218/220, Cmm] and Cmmg, are calculated as detector-measured milliWatts per recorded monitor iode signal value. Cmm] and Cmmg are used to determine the absolute light output into tissue at each wavelength.

Referring again to B, the detector temperature correction module 2206a corrects signal magnitudes for the varying ity of the LEDs by normalizing the temperature-corrected detected s using the LED output signal PDmagm-tude measured by the first monitor photodiode 904 and/or the second monitor photodiode 906. In this case, the Gammon variable for each light source 218/220 from above is amended as follows: GuseCase Gcorrection —_ * PDmagnitude Eqn~ (7) Gnominal - light directionality correction module W0 2018/140984 Referring again to A, the pre-processing subunit 1302 in this aspect includes a light directionality correction module 2208 configured to enable a tion to variations in the detected signals associated with differences in the scattering and absorption of light of different wavelengths through the tissues of the patient 202 during data ition. In one aspect, a correction term for light directionality may be measured by ing data from one or more neous tissue phantoms and using a sensor configuration in which no emission filters are present. The ratio of the signals detected by the first light detector 222 (Bet!) and the signals detected by the second light detector 224 (Det2) measured are used to ine a coefficient Gex or Gem for signals obtained in association with illumination by light at the excitation and emission wavelengths, respectively. The coefficients are used to modify the signal ed by the first light detector 222. In one aspect, the correction of the signals acquired in a homogeneous medium by the first light detector 222 using the coefficients Gex or Gem render the signals measured by the first and second detectors 222/224, as equivalent to within 20% of one r. In other aspects, the correction of the signals acquired in a homogeneous medium by the first light detector 222 using the ients Gex or Gem render the signals ed by the first and second detectors 4 as equivalent to within about 10%, to within about 5%, to within about 2%, and to within about 1%. - detector non-linear response correction module Referring again to A, the pre-processing subunit 1302 in this aspect includes a detector non-linear response correction module 2210 configured to enable a correction to variations in the detected signals associated with non-linear response of the detectors. In this aspect, a calibration curve based on average data may be used to scale the magnitude data obtained by the detectors 222/224. -filter throughput temperature correction (emission) module ing again to A, the pre-processing subunit 1302 in this aspect includes a filter throughput temperature correction (emission) module 2212 configured to enable a correction to variations in the ed signals ated with temperature- dependent optical properties of the optical filter 244 associated with the second light detector 224 during emission-wavelength illumination. In this aspect, the signals Det2 detected by the second light detector 224 may be corrected according to Eqn. (8): W0 2018/140984 Det2 —Det2 (CemF,slopeT(T—Tnom)) Det2 = Eqn. (8) CemF,nom In various aspects, the signal Det2 ed by the second light detector 224 may be monitored while ambient temperature is cycled over a range including the operating ature range or a large enough subset of the range to adequately determine the ature-dependence of the emission filter. These data are acquired with the optical filter 244 installed on the second light detector 224 from a homogeneous, non-fluorescent phantom. Further, simultaneous measurements are monitored from the first light detector 222, and a ratio of the measurements Det2/Bet] is determined. The nominal filter coefficient CemF,nom is calculated as the l ratio of Det2/Bet] obtained at a nominal operating temperatureTnom. In this , the coefficient CemF,slopeT is obtained from the slope of et] obtained over a range of ambient atures during emission- wavelength illumination of the homogeneous, non-fluorescent phantom. - tissue heterogeneity correction module Referring again to A, the pre-processing subunit 1302 in this aspect includes a tissue heterogeneity correction module 2216 configured to enable a correction to variations in the detected signals associated with heterogeneity of the tissues intervening between the first region 206 illuminated by light sources 218/220 and the second and third regions 208/210 at which the light detectors 222/224 are positioned. In this aspect, the signal Det] corrected for light directionality by the light ionality correction module 2208 and the signal Det2 corrected for filter effects by the filter throughput temperature correction (emission) module 2212 are used to calculate Chetm, a coefficient to correct for tissue heterogeneity, ing to Eqn. (9): Chmm = Det2/Bet] Eqn. (9) r throughput temperature correction (excitation) and signal decomposition module ing again to A, the pre-processing subunit 1302 in this aspect includes a filter throughput temperature correction (excitation) module and signal decomposition module 2214 configured to enable a correction to ions in the detected signals associated with temperature-dependent optical properties of the optical filter 244 associated with the second light detector 224 during excitation-wavelength illumination. In W0 2018/140984 this aspect, e the emission filter is configured to block light at the excitation wavelength, the filter throughput temperature correction (excitation) module and signal osition module 2214 performs a correction to variance to the amount of excitation light leakthrough due to temperature-related changes in the optical properties of the optical filter 244. Further, the filter throughput temperature correction (excitation) module and signal decomposition module 2214 enables corrections of the signals measured by the first light detector 222 during excitation-wavelength illumination due to the presence of fluorescence induced by the excitation-wavelength illumination superimposed over the portion of the signal ated with the excitation-wavelength illumination.

In this aspect, the effects of temperature-dependent variation on rough of excitation -wavelength by the optical filter 244 are calculated as expressed in Eqn. (10): CexLT : CexLT,n0m + CexLT,slopeT(T _ Tnom) Eqn~ (10) In this aspect, CexLT,n0m is calculated from the ratio of signals Bet] and Del2 measured from a homogeneous, non-fluorescent phantom at the nominal operating temperature Tnom during excitation-wavelength illumination. sl0peT is calculated as the slope of the signal Del2 measured from a homogeneous, non-fluorescent phantom at a range of operating temperatures T during emission-wavelength illumination.

In this aspect, the filter throughput temperature tion ation) module and signal osition module 2214 further performs a signal extraction to isolate ns of the detected signals associated with diffuse reflectance of the excitation- wavelength illumination and fluorescence. DRexz which is the amount of excitation light ent on the second light detector 224 in the absence of an l filter 244, is not measurable, due to the presence of the optical filter 244. r, the signal Del] measured by the first light detector 222 is a composite signal from both diffuse reflectance of the excitation-wavelength illumination DRexl and fluorescence Flr] . CHetero is obtained using the tissue heterogeneity correction module 2216 as described above. The underlying signals are extracted by use of the following system of equations: Detz : CexLTDRexZ + FlTZ Eqn. (11) Detl : DRexl + FlT‘l Eqn. (12) W0 40984 Flrz : CHeteT-OFlrl Eqn. (13) DRexZ : CHeteroDRexl Eqn~ (14) In this aspect, Fir; is determined by solving the above system of equations using only measurable s Dell and Det2 as demonstrated below: Detz : CexLTCHeteroDRexl + F172 Eqn~ (15) Detz : CexLTCHeter0(Det1 — Flrl) + Flrz Eqn. (16) Detz : CexLTCHeteroDetl _ CexLTCHeteroFlrl + F172 Eqn~ (17) Detz _ CexLTCHeteroDetl : FlT'2(1 _ CexLT) Eqn~ (18) Detz—CexLTCHeteroDetl F172 : Eqn (19) 1—CexLT In this aspect, once Flr2 is obtained as described above, the other signals Flr1,DRex1, and DRexz may be readily obtained through ion into the system of equations (Eqns. (11) — (14)) presented above. -fractionalphoton normalization module Referring again to A, the pre-processing subunit 1302 in this aspect includes a fractional photon normalization module 2218 configured to convert the detector signals, after preprocessing as described above, into units of fractional photons for use in subsequent background subtraction and intrinsic fluorescence correction algorithms as described herein. In this aspect, the detector signals may be converted to urrent by reversing the scaling associated with the ADC and the mpedance amplif1er used to acquire the detected signals to obtain the s in units of photocurrents. Once photocurrent is obtained, a detector responsivity supplied by the light detector’s manufacturer is used to convert the detector urrents to units of Watts. The or signals in Watts are then ratioed to the source power in Watts as measured by additional light detectors 226 used to monitor the output of the light sources 218/220 to obtain the number of fractional photons detected.

W0 2018/140984 - opticalpower correction module Referring again to A and B, the pre-processing subunit 302a in this aspect es a fractional photon normalization module 2218/2218a configured to convert the detector s, after preprocessing as bed above, into units of fractional photons for use in subsequent background subtraction and sic fluorescence correction algorithms as described herein. In this aspect, the detector signals may be converted to photocurrent by reversing the scaling associated with the ADC and the transimpedance amplifier used to acquire the ed signals to obtain the signals in units of photocurrents. Once photocurrent is ed, a detector responsivity supplied by the light or’s manufacturer is used to convert the detector photocurrents to units of Watts.

The detector signals in Watts are then ratioed to the source power in Watts as measured by additional light detectors 226 used to monitor the output of the light sources 218/220 to obtain the number of fractional photons detected. - excitation light leakthrough subtraction module Referring again to B, the pre-processing t 1302a in this aspect includes a fractional photon normalization module 2222 configured to perform an excitation leakthrough subtraction on the Flrmeas signal. To arrive at a fluorescence signal due only to fluorescent photons (Flrphotons ), an excitation leakthrough subtraction is performed. To remove the contribution of excitation light, the excitation leakthrough is taken to be a fraction of the diffuse reflectance excitation (DRexmeas) signal, where a universal calibration factor, CExLT, determines the fraction of the signal to subtract from Flrmeasas expressed below: EXLT : CEXLT * DR exmeas where CExLTis a calibration factor that is obtained by computing the ratio between the tion light detected by both detectors on a non-fluorescing optical m as described below: W0 2018/140984 PCT/U82018/016053 Flrmeas CExLT — DR—_ exmeas This signal is then subtracted from sto provide a fluorescence signal due only to fluorescent photons as expressed below: tons : Flrmeas _ EXLT -fluorescence light leakthrough subtraction module Referring again to B, the pre-processing subunit 1302a in this aspect includes a fluorescence light leakthrough subtraction module 2224a configured to perform a fluorescence leakthrough subtraction on the Flrmeas signal. To obtain the diffuse reflectance, defined herein as the excitation signal due to only excitation photons (DRexphotonS ), a fluorescence leakthrough subtraction is performed. To remove the fluorescence leakthrough, a calibration factor, CFlrLT: was determined based on the relationship between the amount of fluorescence leakthrough observed on a se of human subject data and tissue heterogeneity as measured by the onship between the diffuse reflectance, emission signals (Dilifilt). The relationship is a linear relation as expressed below: DRem C 1 < : * — “T” p p2 lt>+ where p1 and p2 are approximately 0.61 and 0.01, respectively, in one aspect, as determined by the mentioned relationship. In r aspect, p1 and p2 may assume any other value without limitation as defined by the above relationship.

The DRexphotonS signal is then calculated by cting this fraction of measured fluorescence from the e reflectance excitation signal, as follows: DRexphotons : DRexmeaS _ Flrmeas * CFlrLT b) baseline subtraction subunit W0 2018/140984 2018/016053 Referring again to , the processing unit 236 further includes a ne subtraction subunit 1304. In an aspect, the ne subtraction subunit 1304 cts a baseline signal from the light detector measurements to correct for the effects of orescence and light leakage. The baseline period, as used herein, refers to an initial time period of measurements obtained prior to injection of the exogenous fluorescent agent. During the baseline period, the fluorescence signal measured by the system 200 may be assumed to be associated with tissue autofluorescence and/or excitation light from the LED light sources 218/220 leaking h the optical filter 244 of the second light detector 224. In an aspect, the average signal measured during the baseline period, referred to herein as a ne signal, may be subtracted from subsequent cence measurements to yield a measurement associated solely with the fluorescence produced by the exogenous fluorescent agent within the tissues of the patient.

In another aspect, the corrections for excitation light leakthrough and autofluorescence may be implemented in cooperation with the background correction subunit 1306. In this other aspect, rather than subtracting an average signal ed during the baseline period, the background tion subunit 1306 may dynamically calculate the effects of tion light leak-through and orescence at each data acquisition cycle. As a result, subtraction of the effects of excitation light leak-through may be performed prior to the diffuse reflectance correction described herein below, and a subtraction of the effects of autofluorescence may be updated at each data acquisition cycle by the background correction subunit 1306. c) background correction subunit In an aspect, the background correction t 1306 may correct the measured fluorescence data to remove the effects of changes to the optical ties (absorption and scattering) of the tissues of the patient 202 during monitoring of renal extraction of an exogenous fluorescent agent within the tissues of a patient. As described herein above, the optical properties of the tissues may change due to any one or more factors including, but not limited to: vasodilation, vasoconstriction, oxygen saturation, hydration, edema, and any other suitable factor within the region of interest monitored by the system, associated with changes in the concentrations of endogenous fluorophores such as hemoglobin, en, and melanin.

W0 2018/140984 PCT/U82018/016053 In one aspect, the background correction subunit 1306 may determine the intrinsic autofluorescence (IFauw) signal, representing the emission-wavelength light emitted by endogenous fluorophores within the tissues of the patient during data acquisition. In this aspect, the [Fam signal is obtained from the mean or median of [Fbkmd- (the background intrinsic fluorescence data prior to agent ion). The IFbkmd signal is found as s: Flrbkrnd IFbkrnd = DRex DRembkx bkmDRemFiltbkmFilt where the coefficients bkx, bkm, and kmFilt are found via a global error e method.

In one aspect, the values of the powers used in the equation above are determined empirically using a global error surface method. The method in this aspect includes selecting ranges of values for each of the powers (bkx, bkm, bkmFill) for each of the diffuse reflectance signals (DRex, DRem, ‘ltgrgd) selected by a user. In various aspects, the ranges of values for each of the powers may be influenced by any one or more of a variety of factors ing, but not limited to: the design of the system 200, including the design of the sensor head 204, the properties of the selected exogenous fluorescent agent such as excitatory/emission wavelengths, absorption ncy, emission efficiency, and concentration of initial dose in the patient’s tissues, the species of the patient 202 and corresponding concentrations of endogenous chromophores, the position of the sensor head 204 on the patient 202, and any other relevant factor.

In one aspect, the method may include choosing a wide range for each coefficient (bkx, bkm, l) and t a broad search. The error surfaces from this broad search may be analyzed to locate wells in the error surface and the associated ranges for each of the coefficients. The method in this one aspect includes adapting the ranges of each coefficient to include the regions from the broad search within which wells in the error surface were observed and repeating the analysis. This method may be iterated until a suitably fine resolution is achieved that is capable of tely capturing the minimum error.

Step sizes may be selected at 1404 for the ranges of values selected for each power (bkx, bkm, bkmFill). In an aspect, the step size for each factor may be selected based on any one or more of at least several factors including, but not limited to: the anticipated W0 2018/140984 PCT/U82018/016053 sensitivity of the [F values calculated above to s in each factor; a suitable total number of combinations of powers used to calculate IF considered factors including available computational resources, acceptable data processing times, or any other relevant s; and any other suitable criterion for step size.

In various aspects, the step sizes may be the same value for all powers (bkx, bkm, bkmFill). By way of non-limiting example, the step size for all powers may be 0.5. In various other aspects, the step sizes may be constant for all values of a single power (bkx, bkm, bkmFill), but the step sizes selected for each power may be different between different powers. By way of non-limiting example, the selected step size for bkx may be 0.01 and the selected step size for bkm and bkmFl'lZ may be 0.6. In s additional aspects, the step size within one or more of the powers may vary within the range of values for each power. In these various additional aspects, the step size may be reduced within subranges of values for a power for which the IF calculated above is predicted to be more sensitive to small changes in that power. Non-limiting examples of suitable varying step sizes within a range of values for a single power include: different step sizes selected by a user, random step sizes, a linear increase and/or se in step size, a non-linear distribution of different step sizes such as a logarithmic distribution, an exponential distribution, or any other suitable non-linear distribution of step sizes.

The selected ranges of exponents, together with the ed step sizes, may be used to form vectors of potential values of bkx, bkm, bkmFill. For each combination of exponents amongst all vectors, [F is calculated from the ements Flr, DRex, DRem, and DRng’l‘ltgrgd using the above equation. For each combination of exponents, a plurality of [F values are calculated in which each IF value corresponds to one of the data acquisition cycles By way of non-limiting example, using the vectors of potential exponents listed herein above, a total of 405 (5*9*9) pluralities ofIF s would be calculated.

In an aspect, the plurality of combinations of potential exponents may be evaluated to select one combination of nts from the plurality to assign for use in subsequent diffuse reflectance corrections calculated using the above on. An te of error of the corrected Flr signal data (i.e. IF signal data ated using the above equation may be calculated. Any estimate of error may be calculated including, but not limited to, a quantity d to residuals of the IF signal data relative to a curve fit of the IF W0 2018/140984 PCT/U82018/016053 signal data. Any type of known curve-fitting method may be used to curve-fit the IF signal data ing, but not limited to, a single-exponential curve fit. Without being limited to any particular theory, it is thought that the rate of clearance of an exogenous fluorescent agent, such as lVfl3-102, from the kidneys is expected to be a constant exponential decay characterized by the renal decay time constant RDTC.

Intrinsic autofluorescence (IFauw) is then simply the mean or median of IFbkmd~ The autofluorescence signal, Flrauw, is then ted by performing the inverse background diffuse reflectance correction, as s: Flam = IFauw * (DRsrm/ DREEx/DRé’fll-fi” This autofluorescence signal, Flrauw is then removed from the measured fluorescence signal, Flr, to determine the agent intrinsic fluorescence (IFagem) cally representing the emission-wavelength light d by the exogenous fluorescent agent.

Without being limited to any particular theory, the cence measurements obtained by the system 200 that are used to determine renal function include emission-wavelength photons that are detected by the second (filtered) light detector 224.

These emission-wavelength photons are emitted by the exogenous fluorescence agent introduced into the tissues of the patient in response to illumination by excitation- wavelength photons. The emission-wavelength photons travel from the cence source (i.e. the exogenous fluorescence agent) to the second (filtered) light detector 224 through third region 210 of the patient’s skin. However, the emission-wavelength light that is detected by the second (filtered) light detector 224 may also include orescence emitted by nous chromophores such as keratin and collagen within the tissues of the patient, as well as hrough of excitatory-wavelength light through the optical filter 244 of the second light detector 224. The excitation-wavelength photons that induce fluorescence of the exogenous fluorescent agent are produced by the first light source 218 and are directed into the first region 206 of the patient’s skin. If the optical properties of the patient’s skin (scattering and/or absorption) varies over the time interval at which the or data used to determine renal function is acquired (i.e. from a few hours to about 24 W0 2018/140984 PCT/U82018/016053 hours or more), the accuracy of the fluorescence measurements may be impacted, as discussed previously above.

During each measurement cycle in an aspect, the system 200 may direct light into the first region 206 of the patient’s skin with a pulse of emission-wavelength light and a pulse of tion-wavelength light in an alternating series and may detect all light emerging from the second region of the patients skin using the first (unfiltered) light detector 222 and a portion of the light emerging from the third region 210 of the patient’s skin using the second (filtered) light detector 224. The light intensity detected by each ation of tion and emission ngth illumination of the first region 206 and detection by the unfiltered/filtered light detectors 222/224 contain information not only about the tration of the exogenous fluorescent agent in the t’s tissues, but also information about the optical properties of the patient’s skin.

Table 2.‘ Light Detector Measurements After Temperature and Power Fluctuation Corrections Illumination First (Reference) Second (Primary) wavelength Light or Light Detector Unfiltered Filtered The primary measurement of fluorescence is Flrmeas the , intensity of fluorescent light measured at the filtered detector.

The diffuse reflectance measurement Flrmeas represents the propagation of photons to the non-filtered arm and is composed primarily of tion photons.

DRemand DRemlfl-lteredrepresent the propagation of emission-only photons.

Referring to Table 2, light intensity measured by the second ed) light detector 224 during illumination by the excitation-wavelength light captures the raw W0 2018/140984 PCT/U82018/016053 intensity of light emitted by the exogenous fluorescent agents (Flrmeas) prior to any corrections for tissue optical properties in various aspects. After baseline subtraction corrections as described herein previously, the emission-wavelength light contained in Flrmeasis assumed to originate predominantly from the exogenous fluorescent agent, with only minor butions due to auto-fluorescence by endogenous fluorophores, and is ore termed Flragent. In an aspect, if no change in the optical properties of the patient’s skin is assumed, all autofluorescence butions would be subtracted off during the baseline correction described herein above.

However, if the optical properties of the t’s skin change during the acquisition of data, slightly more or less of the orescence may emerge from the patient’s skin at the emission wavelength, thereby introducing uncertainty into the accuracy of the background ction correction performed previously. In addition, varied skin optical properties may further alter the ity of light at the excitation wavelength reaching the exogenous fluorescent agent, thereby altering the amount of energy absorbed by the exogenous fluorescent agent and the intensity of induced fluorescence from the exogenous fluorescent emitted in response to illumination by the excitation-wavelength light. In various aspects, the remaining three light measurements enable monitoring of the optical ties of the patient’s skin and provide data that may be used to adjust for any changes in the optical properties of the patient’s skin including the effects of autofluorescence and excitatory-wavelength light bleed-through.

Referring again to Table 2, light intensity measured by the first (unfiltered reference) light detector 222 during illumination by excitation-wavelength light captures a measure of the diffuse ance of excitation-wavelength light propagated through the t’s skin (DRex). Although the first light detector 222 is configured to detect both excitation-wavelength and emission-wavelength light, the intensity of the excitation- wavelength light is orders of magnitude higher than the ity of the emission- wavelength light as a result of the lower efficiency of producing light via fluorescence. In various aspects, the proportion of emission-wavelength light within DRexis assumed to be negligible. In other aspects, the proportion of emission-wavelength light within DRex is estimated and subtracted. t being limited to any particular theory, because the ity of the excitation-wavelength light directed into the patient’s skin is d to be relatively constant with ible losses due to absorption by the exogenous fluorescent W0 2018/140984 PCT/U82018/016053 agent, and is subject to power corrections as described herein usly, DRex serves as a benchmark measurement to assess changes in the optical properties of the patient’s skin with respect to the excitation-wavelength light.

Light intensity measured by the first ered reference) light detector 222 during illumination by emission-wavelength light captures a measure of the diffuse ance of emission-wavelength light propagated through the patient’s skin (DRem).

Without being limited to any particular theory, because the exogenous fluorescent agent is not d to emit emission-wavelength light due to the absence of excitation-wavelength illumination during this phase of the data acquisition cycle, and because the intensity of the emission-wavelength light directed into the patient’s skin is relatively constant and subject to power corrections as described herein previously, DRex serves as a benchmark measurement to assess changes in the optical properties of the patient’s skin with respect to the emission-wavelength light.

Light intensity measured by the second ed) light or 224 during illumination by emission-wavelength light captures a second measure of the diffuse reflectance of emission-wavelength light propagated through the patient’s skin (DRgmflltgrgd). In one aspect, DRng’l‘ltgrgd is subject to the same assumptions as DRem as described herein above. In addition, tgrgd provides a means of assessing heterogeneity of the tissue’s optical properties. Because DRemflmgd is measured by the second light detector 224 configured to detect light emerging from the patient’s skin at the third region 210 (see , the intensity of light measured in DRgmflltgrgd has propagated along an l path through the skin of the patient that is different from the optical path led by the light measured in DRem. Without being limited to any particular theory, e the distances of the first detector aperture 1004 and second light aperture 2006 h which light is delivered to the first and second light detectors 222/224, respectively are designed to be equidistant from the light delivery aperture 1002 (see ), any differences between DRemflltgrgd and DRem are assumed to be a result of heterogeneity on the optical properties of the skin traversed by the two ent optical paths. excitation-wavelength light leak-through correction In one aspect, DRexmeas serves as a basis for the estimation of leak-through of excitatory-wavelength light into the second (filtered reference) light detector 224 used as W0 40984 PCT/U82018/016053 part of the method of ng the effects of variation in background signal described herein. Without being limited to any particular theory, it is assumed that the amount of leak-through of excitatory-wavelength light into the second (filtered reference) light detector 224 is tional to the DRM signal, and that this proportion is influenced exclusively by device-related factors, rather than factors related to the optical properties of the patient’s skin. As a result, the proportion of the DRM signal representing leak-through light is assumed to be constant, as described herein below.

In one aspect, the excitation-wavelength light leak-through (ExLI) included within the raw fluorescence signal (FIt") is assumed to be a constant fraction CExLT of the DRexmeas signal according to Eqn. (21): EXLT : CEXLT * DR exmeas Eqn. (21) where CEx” is a sensor-head specific calibration factor.

In one aspect, CEx” is obtained by computing the ratio between the excitation light detected by first and second light detectors 4 (Dell/Del2) on a non- fiuorescing optical phantom ing to Eqn. (22): Det2 Flrmeas CExLT = _ _ Eqn. (22) Detl DRexmeas In another aspect, the excitation light reaching the filtered detector is d to be different than the light reaching the non-filtered detector due to tissue heterogeneity. In this aspect, the ratio of the emission-wavelength light at each detector is used to t for this heterogeneity.

In various aspects, the calibration factor CExLT may be specific to an individual sensor head 204 or CEx” may be applicable to all sensor heads 204 of a system 200 depending on various factors including, but not limited to, cturing nces.

In an aspect, if the system 200 is used to obtainCExLT, s and DRexmm are from a non-fluorescent, homogeneous phantom in the context of the system 200 as described herein above. It is to be noted that Eqn. (22) assumes that the tissue monitored by the system 200 is homogeneous.

W0 2018/140984 PCT/U82018/016053 In an aspect, the excitation-wavelength light leak-through (ExLT) determined by Eqn. (21) may be subtracted from the raw fluorescence signal (Flrmeas) to obtain a corrected fluorescence signal ns as described in Eqn. (23): Flrphowns = Flrmeas — ExLT Eqn. (23) A is a graph of a raw fluorescence signal (Flrmeas, blue line) and the corresponding excitation-wavelength light hrough (ExLT, red line) determined using Eqn. (23) obtained by a system 200 in one aspect before and after the injection of an exogenous fluorescence agent. As illustrated in A, the ExLT signal varies over the course of data ition. B is a graph comparing the raw fluorescence signal (Flrmeas, blue line) and the fluorescence signal with the excitation-wavelength light leakthrough removed (FlrphotonST, green line) as described herein above in Eqn. (23).

In one aspect, the raw fluorescence signals s are first corrected to remove the effects of tion-wavelength light leak-through using Eqn. (23). In this aspect, subsequent corrections to remove the effects of autofluorescence are implemented using the corrected fluorescence signal Flrphotonsas a basis as described herein below. fluorescence leak-through correction Without being limited to any particular theory, the light detected by the unflltered light detector during illumination by light at the excitatory wavelength is a mixture of diffuse reflectance of the excitation wavelength light and light from agent fluorescence. In one aspect, the diffuse reflectance is d to be sufficiently more intense than the fluorescence such that the contribution of fluorescence to the unflltered detector ement was negligible.

In another aspect, the contribution of fluorescence to the red detector measurement may be non-negligible. By way of non-limiting e, is a graph showing DRexmeas and Flrmeas over a full day in the absence of administration of an ous fluorescent agent. However, as illustrated in , the DRexmm signal occasionally showed leak h of fluorescence, as evidenced by a correlated signal rise after agent administration into the patient’ s bloodstream.

W0 2018/140984 PCT/U82018/016053 In one aspect, the portion of the e ance excitation signals due only to excitation photons are removed from the raw DRexmeassignal according to Eqn. (24): DRexphotons : DRexmeaS _ Flrmeas * CFlrLT Eqn~ (24) In various aspects, the coefficient CFM” is empirically determined using the relationship between a measured amount of fluorescence leak-through (Flrleakthmugh) on DRemFilt the DRexmeaS signal, in relation to tissue heterogeneity as expressed by the ratio DRem (see discussion below). In one aspect, the measurements may be obtained from a plurality of subjects. By way of miting example, is a graph summarizing a DRem relationship between empirically determined Flrleakthmugh and d from a DRemFth database of 33 ts. In this aspect, this empirically-derived relationship was checked on multiple patient datasets and found to be consistent. The correction coefficient CFlrLT was set to incorporate the relationship between tissue heterogeneity and amount of fluorescence leak through, as defined below: CFlrLT = P1 * (—DRW::.”) + p2 Eqn. (25) In one , Eqn. (25) includes p1 =0.6l38 and p2 = 0.01095, as determined by a bisquares weighted linear fit to the relationship illustrated in .

In another aspect, CFITLT is determined by obtaining measurements on optical phantoms provided with increasing fluorescence concentrations, where the only change signals is due to tration of exogenous fluorescence agent concentration. ion of fluorescence and excitation wavelength diffuse reflectance In various aspects, the number of photons due to either DRex or Flr on either the filtered or unfiltered detector depends on light directionality and the gain of each detector at the detected wavelength, as shown below: DRexmeaS = A1 * DRexphotonS + B1 * Flrphotons Eqn. (26) s : A2 * otons + 32 * Flrphotons Eqn~ (27) W0 2018/140984 PCT/U82018/016053 where the coefficients A1, A 2, B1, and B2 include a directionality and gain factor. By way of non-limiting example, A1 may be provided in the form of Eqn. (28): A1 = d4505PM1 * GSPM1@450 Eqn. (28) where_d4505PM1 and GSPM1@450are directionality and gain factors of a detector SPMI at an illumination wavelength of 450 nm.

In one aspect, the photon s may be isolated as expressed in Eqns. (29) and (30): B A A Bl (B—i _ (Ti) tons : Flrmeas _ 1T: Rexmeas Eqn. (29) A B B A2 (I: — 57:) own, = DRexmm — B—:Flrmeas Eqn. (30) In various aspects, the constant terms in front of the photon signals, such as B A B1 (B—Z—A—Z) . . . are not , because the renal functlon. rs as d1sclosed here1n 1 1 measure rates of change of intrinsic fluorescence (IF) as expressed by Eqn. (31): IF = C0 + Cle't/T —> log(IF) = log(C1) —% Eqn. (31) In one aspect, the terms % (or CM”) and g (or CFlrLT) are determined 1 2 experimentally to isolate Flrphotons and DRexphotonS, respectively, as described above. autofluorescence correction In various aspects, the method of correcting the measured fluorescence to remove the time-varying effects of background may r include removing the effects of autofluorescence in addition to removing the effects of excitation-wavelength leak-though.

Autofluorescence, as used herein, refers to the emission-wavelength light produced by endogenous chromophores, such as keratin and collagen, in response to illumination by excitation-wavelength light. In s aspects, autofluorescence may vary over the course of acquiring fluorescence measurements using the systems and s described herein.

Without being limited to any particular theory, changes in the optical properties of the W0 2018/140984 patient’s skin, such as s in the concentration of chromophores such as hemoglobin and/or melanin may cause variation in autofluorescence levels. is a graph summarizing the measurements of raw fluorescence (Flrmeas, blue line) obtained during the background interval, defined herein as the interval prior to injection of the exogenous cent agent into the patient 202, when the patient’s tissues are assumed to contain no exogenous cent agents. Also shown in is the signal resulting from the removal of the effects of hrough of excitatory- wavelength light (ExLI) from Flrmeas using Eqn. (5) as described herein above. The remaining fluorescence signal detected during the background interval, shown as a green line in , may be assumed to be attributable to autofluorescence in various aspects.

In one aspect, the intrinsic autofluorescence (IFauw), defined here as the measured fluorescence at the emission wavelength attributable only to emission by endogenous chromophores, such as n and collagen, may be calculated as the median value of the corrected fluorescence signal Flrphotons (see Eqn. 23) obtained during the background interval according to Eqn. (32).

IFAutO = median(Flrph0t0nS(1: endBackgroundD Eqn. (32) where endBackground is the index of the data acquisition in the dataset ponding to the end of the background interval just prior to injection of the exogenous fluorescent agent.

In an aspect, the orescence may be assumed to be relatively stable throughout the entire data acquisition process, including the interval ing injection of an exogenous fluorescent agent. In this aspect, the effect of autofluorescence may be removed by subtracting the IFAutO value obtained in Eqn. (32) from the corrected fluorescence signal Flrphotonsas expressed in Eqn. (33): [Fagent : Flrphotons _ IFAuto Eqn~ (33) where [Fagem denotes the intrinsic cence specifically representing the emission- wavelength light emitted by the exogenous fluorescent agent.

W0 2018/140984 PCT/U82018/016053 A is a graph summarizing various measurements obtained during the background al: raw fluorescence (Flrmeas), DRexmeas (red line), DRem (orange line), and DRemfltmd (purple line). In on, the intrinsic autofluorescence (IFauw, green line) calculated using Eqn. (32) is also shown in A. During the background interval shown in A, all quantities were vely stable in value.

B is a graph summarizing the diffuse ion ements shown in A: DRexmeas (red line), DRem (orange line), and DRgmflltgrgd (purple line).

Over the course of obtaining fluorescence measurements after injection of the exogenous fluorescent agent (i.e. after a time of about 9:07 as shown in ), the e reflection measurements decrease signif1cantly, indicating that the optical properties of the patient’s skin, which impact the measured signal from autofluorescence, may also change during this time period.

In an additional aspect, diffuse reflectance measurements may be used to project the underlying autofluorescence signal for the full ement period, thereby accounting for changes in the l properties of the patient’s skin over the full course of data measurements. In one , diffuse reflection measurements may be used to scale the corrected fluorescence signal Flrphotons to account for changes in the optical properties of the patient’s skin, resulting in an intrinsic fluorescence. In this aspect, to t the fluorescence measurements obtained after the injection of the ous fluorescence agent, the intrinsic autofluorescence (IFauw) calculated from Eqn. (32) may be subtracted from the combined sic fluorescence [FAggnmndAutg obtained from Eqn. (33), as expressed in Eqn. (34): [Fagent : IFAgentAndAuto _ IFAuto Eqn~ (34) In one aspect, the background correction subunit 1306 may enable a background correction method 2000 as summarized in the block diagram of . The method 2000 may include performing a correction at 2002 to remove the s of the leak-through of emission-wavelength light into the second (filtered reference) light detector 224 as described in Eqns. (29), (30), and (31) above. The method 2000 may further include estimating the level of autofluorescence (IFauw) at 2004 from an analysis of the measurements obtained during the background interval as described in Eqn. (32) above.

W0 2018/140984 PCT/U82018/016053 The method 2000 may additionally include performing a correction at 2006 to remove the effects of autofluorescence from the fluorescence measurements as described in Eqn. (33) above. In effect, the autofluorescence signal [Fame is projected forward into subsequent fluorescence measurements and is removed at 2006. The intrinsic fluorescence [Fagem resulting from the removal of background effects from the raw fluorescence measurements may transformed by the RDTC calculation subunit 1310 as described herein below into a parameter including, but not d to, glomerular ion rate (GFR) and/or renal decay time constant (RDTC) representing renal function. e) fault detection subunit Referring again to , the sing unit 236 of the controller 212 may further include a fault detection subunit 1312 configured to monitor the function of the light sources 218/220 and light detectors 222/224 and to inform the user of any irregularities of any detected faults within the system 200 via the y unit 216. In various s, the fault detection subunit 1312 may enable the basic identification of fault and notice states by examining the signal levels ed from the light sources 218/220 and light detectors 4 and associated additional temperature sensors 228 and additional light detectors 226 of the sensor head 204 (see . In various aspects, the signal magnitudes (see Eqn. (1)) and average signals may be used to determine the peak and nadir levels of the modulation of the LED light sources 218/220. The nadir of the signal, defined herein as the average signal minus half the peak-to-peak signal, may be used to monitor ambient light levels in one . t being limited to any particular theory, additional contributions to the nadir levels of the modulated signals, such as er DC offset, may be neglected as small and constant relative to the contributions of ambient light leakage. In an aspect, if the detected ambient light levels register in excess of about one quarter of the high-speed ADC 1102 range at low or amplifler gain, an ambient light notice is issued to the user via the display unit 216.

In various other aspects, saturation of the light detectors 4 detectors may also be monitored by the fault detection subunit 1312. In these other aspects, the saturation may be monitored by calculating the peak value of the , defined herein as the average signal value plus half the peak-to-peak signal. If the signal’s peak value falls within is within 5% of saturation of the ADC range, the fault detection subunit 1312 may W0 40984 PCT/U82018/016053 issue a saturation notice to the user via the display unit 216. If saturation event is detected by the fault detection subunit 1312, the ambient light level may then be checked to determine if the saturation event is associated with ambient light saturation, defined herein as a saturation event occurring concurrently with an ambient light notice as described herein above. If an ambient light saturation event is detected, the fault detection subunit 1312 issues an ambient light saturation notice to the user via the display unit 216, and data ition by the acquisition unit 234 is continued in this notice state to allow the user to resolve the ion. If a saturation event is detected that is not associated with an excess of ambient light, the fault detection subunit 1312 may signal the light detector control unit 232 to perform an adjustment of detector gain and/or may signal the light source control unit 230 to perform an adjustment to the LED t source 1126 to adjust LED intensity.

In various aspects, the fault detection subunit 1312 issues a notification to the user via the display unit to report either the ambient light saturation event, or the saturation event not associated with an excess of ambient light. In some aspects, if a tion event is detected, but the automatic gain adjustment has been disabled by a user when the system 200 is configured in the ering Mode as described herein above, the user is also notified via the display unit. e) post-agent administration selection subunit Referring again to , the processing unit 236 may further include a post-agent administration subunit 1308 configured to automatically identify the portion of the measurement data set that corresponds to an gent administration region, as described herein below. is a graph of fluorescence ements obtained from a t over a period of about 10 hours after injection of an exogenous cence agent such as 1Vfl3-102 after a pre-injection period 2102 of about 3 hours. Referring to , the pre- injection/baseline period 2102 is characterized by a relatively low and stable fiuorescence level, likely due the absence of exogenous fiuorescent agent in the blood of the patient.

After the injection 2103 of the exogenous fiuorescence agent, the fluorescence measurements exhibit a sharp increase 2106 to a peak concentration 2108, followed by a relatively smooth exponential decrease back to ound fiuorescence levels at the s eliminate the exogenous fiuorescence agent from the blood of the patient.

W0 2018/140984 PCT/U82018/016053 Without being limited to any particular theory, it is thought the ed exogenous fluorescence agent is likely well-mixed after an amount of time in the exponential concentration decrease has elapsed.

Referring again to , after an exogenous fluorescent agent, such as 1Vfl3-102, is injected into the bloodstream of a patient, the exogenous fluorescent agent undergoes an equilibration period of diffusion from the bloodstream into the rest of the extracellular tissues of the patient. After agent ion 2103, the temporal profile of the fluorescence signal IF may be characterized as a two-exponential signal profile described by Eqn. (35): IFpre—equilibration : C0 + 616—”?1 + Cze—t/TZ Eqn~ (35) in which C0 is the baseline signal that is typically removed by baseline subtraction as described herein above.

Referring again to , once the diffusion of the exogenous fluorescent agent into the extracellular tissues of the patient reaches a quasi-steady state condition, quilibration 2110 is achieved and the fluorescence signal may be characterized as a linear decay. Without being limited to any ular theory, the quilibration region 2110 of the measurement data set is assumed to be terized as a temporal region of the IF dataset that when log-transformed, is well-described by a linear equation. In one aspect, the post-equilibration region is escribed bed by Eqn. (3 6): IFpost—equilibration : C0 + Cle—t/T Eqn~ (36) In an aspect, the post-agent administration selection subunit 1308 may identify the quilibration period 2110 automatically by performing a single-exponent curve fit at different portions of the IF data set and analyzing the associated curve fitting errors for each of the different portions. In various aspects, the gent administration selection subunit 1308 may select the earliest-occurring portion of the IF data set in which the curve-flt error associated with a single-exponent curve fit falls below a threshold value as the initial post-equilibration portion of the IF data set suitable for data correction and analysis as described herein above. Any is method le for comparing curve-fit errors association with single-exponential curve fits of different portions of the IF data set W0 2018/140984 may be used in the post-agent administration selection subunit 1308 including, but not limited to, linear curve-fitting portions of the IF data set falling within overlapping or non- pping data windows and comparing the curve-fit errors of the ponding data windows. In an aspect, the post-agent administration selection subunit 1308 may produce at least one signal configured to signal the time range within the IF data set corresponding to the post-equilibration period 2110 to the RDTC calculation subunit 1310 to enable the selection of a suitable portion of the IF data set to correct and analyze as disclosed herein.

In r aspect, a linear fit and a 2-exponential fit to the IF data may be compared. In this other aspect, equilibration may be identified as complete once the fitting error is equivalent (corrected for the extra degrees of freedom in the nential fit). fl RDTC calculation subunit In various aspects, the system 200 is configured to transform the various measurements from the light detectors 222/224 and associated light sources 0 and other l and light sensors into a corrected intrinsic cent (IF) signal corresponding to the detected fluorescence attributable solely to emission of fluorescence by the exogenous fluorescent agent at the emission wavelength in response to illumination by light at the tory wavelength. In various aspects, the exponential decrease of the IF signals during the post- agent administration portion of the IF data set may be analyzed to monitor and quantify renal function.

In one aspect, the exponential decrease of the IF signals during the post- agent stration portion of the IF data set may be transformed into a glomerular filtration rate (GFR) configured to quantify renal function. In another aspect, the exponential decrease of the IF signals during the post-equilibration portion of the IF data set may be ormed into a renal decay time nt , also configured to quantify renal function. In another aspect, the exponential decrease of the IF signals during the post-equilibration portion of the IF data set may be transformed into a renal decay rate, also configured to quantify renal function.

Referring again to , the processing unit 236 may r include an RDTC calculation subunit 1310 configured to automatically orm the IF signals into a renal decay time constant (RDTC). As used herein, renal decay time constant (RDTC) is W0 2018/140984 PCT/U82018/016053 defined as the time constant associated with the post-equilibration single-exponential decay described in Eqn. (36) herein above. In one aspect, after accurate baseline subtraction by the baseline subtraction subunit 1304, the renal decay time constant I may be calculated by performing a linear regression on the log-transformed IF signal data (log (IF)), as described in Eqn. (37): log(IF) = ) — it Eqn. (37) In various aspects the RDTC ation subunit 1310 may produce signals configured to produce a display of the calculated RDTC using the display unit 216. The display of the calculated RDTC may be provided to the display unit 216 in any suitable format ing, but not limited to: a graph of RDTC as a function of time, a single discrete RDTC value, a table of RDTC values as a function of time, a coded y or other graphical representation configured to specific whether the calculated RDTC may be classified as normal/healthy, abnormal, high, low, and any other suitable classification.

In various other aspects, any of the graphical formats described above may be continuously or non-continuously updates as additional data is obtained and analyzed. In one aspect, the RDTC calculation subunit 1310 may calculate RDTC as described herein above within non-overlapping and/or overlapping windows within the IF data set.

In another aspect, the RDTC calculation t 1310 may t RDTC into ular ion rate (GFR) using known methods. In this aspect, RDTC may be inverted and multiplied by a slope, resulting in cGFR, a prediction of GFR that may be corrected for body size (e.g. body surface area, or volume of distribution). v) Memory Referring again to the controller 212 of the system 200 may further include a memory 242 red to facilitate data storage in the system 200. In some ments, the memory 242 es a plurality of storage components such as, but not limited to, a hard disk drive, flash memory, random access memory, and a magnetic or optical disk. Alternatively or additionally, the memory 242 may include remote storage such a server in communication with the controller 212. The memory 242 stores at least one computer program that, when received by the at least one processor, cause the at least one processor to perform any of the functions of the controller 212 described above. In one W0 2018/140984 PCT/U82018/016053 implementation, the memory 242 may be or n a computer-readable medium, such as a floppy disk device, a hard disk device, an optical disk device, or a tape device, a flash memory or other similar solid state memory device, or an array of devices, including devices in a e area network or other configurations. A computer program product can be tangibly embodied in an information carrier. The computer program product may also contain instructions that, when executed, perform one or more functions, such as those described herein. The information carrier may be a ansitory computer- or e- readable , such as the memory 242 or memory on the processor 238.

In various s, the system 200 may record raw measurements and processed data to a series of files. Each file may contain a header, which contains information about the operator, instrument, and session. Each experimental session records a set of files into a separate folder for each sensor head used in that session. The raw data file may contains in-phase, quadrature, and average measurements from the detectors and monitors during the active periods of both the excitation wavelength and the on wavelength LEDs, along with the gain settings of the LEDs and detectors at the time of data acquisition.

In various other aspects, the processed data file may contain the fluorescence and diffuse reflectance measurements after magnitude calculation and correction for the monitor readings, along with the gain settings of the LEDs and detectors.

The intrinsic fluorescence data file may contain the intrinsic fluorescence measurements resulting from the diffuse reflectance correction of the raw fluorescence signals. The GFR file may n the calculated GFR as a function of time, classified to te whether post-equilibration has occurred, along with confidence bounds. The telemetry file may n the temperature and e measurements. The event record file may contain both user and automatically generated event records. v1) GUI Unit ing again to the controller 212 may include a GUI unit 240 configured to e a plurality of signals ng various measured and transformed data from other units of the system in various aspects. In addition, the GUI unit may be configured to produce signals configured to operate the display unit 216 in order to display W0 2018/140984 data, frames, forms, and/or any other communications of information between the user and the system 200. viz) Processor Referring again to the controller 212 may further include a processor 238. The processor 238 may include any type of conventional processor, microprocessor, or processing logic that interprets and executes instructions. The processor 238 may be configured to process instructions for execution within the controller 212, including instructions stored in the memory 242 to display graphical information for a GUI on an al input/output device, such as display unit 216 coupled to a high speed interface. In other implementations, multiple processors and/or multiple buses may be used, as appropriate, along with multiple memories and types of memory. Also, multiple controllers 212 may be ted, with each device providing portions of the necessary operations to enable the functions of the system 200. In some ments, the processor 238 may e the acquisition unit 234, the light detector control unit 232, the light source control unit 230, and/or the processing unit 236.

As used herein, a processor such as the processor 238 may include any programmable system including s using micro-controllers, d instruction set ts (RISC), application specific integrated circuits (ASICs), logic circuits, and any other circuit or processor capable of executing the functions described herein. The above examples are example only, and are thus not intended to limit in any way the definition and/or meaning of the term “processor.” As described herein, computing devices and computer systems include a processor and a memory. However, any processor in a er device ed to herein may also refer to one or more processors wherein the processor may be in one computing device or a plurality of computing devices acting in parallel. Additionally, any memory in a computer device referred to herein may also refer to one or more memories n the memories may be in one computing device or a plurality of computing devices acting in parallel.

W0 2018/140984 PCT/U82018/016053 C. Operation Unit The operation unit 214 may be configured to enable a user to interface (e.g., visual, audio, touch, button presses, stylus taps, etc.) with the controller 212 to l the operation of the system 200. In some embodiments, the operation unit 214 may be further coupled to each sensor head 204 to control the ion of each sensor head 204.

D. Display Unit Referring again to the system 200 may further include a display unit 216 red to enable a user to view data and l information of the system 200.

The display unit 216 may further be coupled to other components of the system 200 such as the sensor head 204. The display unit 216 may include a visual display such as a cathode ray tube (CRT) display, liquid crystal y (LCD), light emitting diode (LED) display, or “electronic ink” display. In some embodiments, the display unit 216 may be configured to present a graphical user interface (e. g., a web browser and/or a client application) to the user. A graphical user interface may include, for example, an display for GFR values as described herein above as produced by the system 200, and operational data of the system Exogenous Markers Without being limited to any particular theory, molecules which are highly hydrophilic and small (creatinine, molecular weight = 113) to moderately sized (inulin, molecular weight ~5500) are known to be rapidly cleared from systemic circulation by glomerular filtration. In addition to these properties, an ideal GFR agent would not be reabsorbed nor secreted by the renal tubule, would exhibit ible binding to plasma ns, and would have very low toxicity. In order to design optical probes that satisfy all of these ements a balance was struck between photophysical properties, and the molecular size and hydrophilicity of the fluorophore. For e, while hydrophobic cyanine and indocyanine dyes absorb and emit optimally within the near infrared (NIR) biological window (700-900 nm), hilicity is not sufficiently high to function as pure GFR agents. Smaller dye molecules may be more easily ted to the extremely hydrophilic species required for renal clearance, but the limited at-systems resulting from W0 40984 PCT/U82018/016053 these lower molecular weight compounds generally enable one photon tion and emission in the ultraviolet (UV).

To e the pharmacokinetic issues in concert with enhancing the photophysical properties, simple derivatives of 2,5-diaminopyrazine-3,6- dicarboxylic acid act as very low molecular weight fluorescent scaffold s with bright emission in the yellow-to-red region of the electromagnetic spectrum. SAR studies have been carried out using amide-linked variants of these derivatives for the simultaneous optimization of GFR pharmacokinetics and hysical properties. A variety of hilic functionalities for enabling rapid renal clearance of this class of pyrazine fluorophores including carbohydrate, alcohol, amino acid and s PEG-based linker strategies may be employed. PEG substitution maybe used to increase hydrophilicity and solubility, reduce toxicity, and modulate ation of the resulting pyrazine derivatives. Variations of molecular weight and architecture (and hence hydrodynamic volume) in a series of moderately sized PEG-pyrazine derivatives may also be suitable for use as endogenous fluorescent agents.

In one aspect, the ous fluorescent agent is lVfl3-102.

EXAMPLES The following example illustrates various aspects of the disclosed systems and methods.

Example I .' Sensor Head with Flared Housing is a perspective view of a sensor head 204a in another . In this other aspect, the sensor head 204a includes a g 600a formed from an upper housing 602a and a flared lower housing 604a. The surface area of the lower housing 604a expands to form an enlarged bottom surface 608a. The housing 600a further includes a cable opening 806a formed through the upper housing 602a. is a bottom view of the sensor head 204a showing the bottom surface 608a of the housing 600a. The bottom surface 608a may include an aperture plate 702a including one or more apertures 704a configured to transmit light between the skin of the patient and the light sources and light detectors contained inside the housing 600. As W0 2018/140984 illustrated in , the apertures 704a include a light delivery aperture 1002a configured to deliver illumination produced by the first and second light sources 218/220 to tissues of the t 202, as well as first and second detector res 1004/1006 red to receive light from the tissues of the t 202. In one aspect, the bottom surface 608a enables the positioning of the apertures 704a beneath a relatively large area obscured from t light conditions by the bottom e 608a. This reduction of scattered ambient light entering the first and second detector apertures 1004/1006 reduces noise introduced into the light intensity measurements obtained by the first and second light detectors 222/224.

In various aspects, the bottom surface 608a of the housing 600a may be attached the patient’s skin using a biocompatible and transparent adhesive material 610a including, but not limited to, a clear double-sided medical grade adhesive, as illustrated in . The arent adhesive material 610a may be positioned on the bottom surface 608a such that the adhesive material 610a covers the apertures 704a. is an isometric view of the sensor head 204a with the upper housing 602a and various electrical components removed to expose an inner housing 2502. is an exploded view of the inner housing 2502 and associated electrical components illustrated in . ing to and , the inner g 2502 is contained within the housing 600a and is mounted to the lower g 608a. The inner housing 2502 contains a sensor mount 912 with a first detection well 908, a second detection well 910, and a light source well 902 formed therethrough. The first light detector 222 is mounted within the first detection well 908 and the second light or 224 is d within the second detection well 910. The first and second light sources 218/220 are mounted within the light source well 902. In an aspect, the first detection well 908, second detection well 910, and light source well 902 of the sensor mount 912 are optically isolated from one r to ensure that light from the light sources 218/220 does not reach the light detectors 222/224 without coupling through the skin of the patient 202. The separation between the two detection wells 908/910 ensures that the detected fluorescence signal from the exogenous fluorescent agent is distinguishable from the unfiltered excitation light, as described in detail above.

W0 40984 2018/016053 Referring to , the inner housing 2502 includes a first ion aperture 2602, second detection re 2604, and light source aperture 2606. The sensor mount 912 is coupled to the inner housing 2502 so that the first detection aperture 2602, second detection aperture 2604, and light source aperture 2606 are aligned with the first detection well 908, second detection well 910, and light source well 902 of the sensor mount 912, respectively.

In one , optically transparent windows 2610, 2612, and 2614 are coupled within first detection aperture 2602, second detection re 2604, and light source aperture 2606, respectively, to seal the apertures while also providing optically transparent conduits between the tissues and the interior of the sensor head 204a. In addition, diffusers 2616, 2618, and 2620 are coupled over optically transparent windows 2610, 2612, and 2614, respectively. The diffusers 2616, 2618, and 2620 are provided to spatially homogenize light delivered to the tissues by light sources 218/220 and to spatially homogenize light detected by light detectors 222/224. In an aspect, the absorption filter 244 is coupled to the diffuser 2616. In one aspect, an optically transparent adhesive is used to couple the absorption filter 244 is d to the er 2616.

In view of the above, it will be seen that the several advantages of the disclosure are achieved and other advantageous results attained. As s changes could be made in the above s and systems without departing from the scope of the sure, it is intended that all matter contained in the above description and shown in the accompanying drawings shall be interpreted as illustrative and not in a limiting sense.

When introducing elements of the present disclosure or the various versions, embodiment(s) or aspects thereof, the articles (4 77 (L a an”, “the” and “said” are intended to 77 (L mean that there are one or more of the elements. The terms “comprising 7 including” and “having” are intended to be inclusive and mean that there may be additional elements other than the listed elements.

Claims (12)

What is claimed is:

1. A method of monitoring a time-varying fluorescence emitted from a fluorescent agent from within a diffuse reflecting medium with time-varying l ties, the method comprising: providing a measurement data set comprising a plurality of measurement entries, each measurement data entry comprising at least two measurements obtained at one data acquisition time from a patient before and after administration of the fluorescent agent, the at least two measurements comprising an = ∗−(1: signal detected at a third region adjacent to the diffuse ting medium by a filtered light or during illumination of the diffuse reflecting medium by excitatory-wavelength light from the first region, and at least one DR signal selected from: a = signal detected at a second region adjacent to the diffuse ting ∗−(1 medium by an ered light detector during illumination of the diffuse reflecting medium by excitatory-wavelength light from a first region adjacent to the diffuse reflecting medium; a DR em signal detected at the second region by the unfiltered light or during illumination of the diffuse reflecting medium by emission-wavelength light from the first position; and a DR em,filtered signal detected at the third region by the filtered light detector during illumination of the diffuse reflecting medium by emission-wavelength light from the first on; and; identifying a gent-administration portion of the measurement data set; transforming each = ∗−(1: signal of each measurement data entry within the post-agentadministration portion of the measurement data set to an IF agent signal representing a detected fluorescence intensity emitted solely by the fluorescent agent from within the diffuse reflecting medium, wherein the transforming comprises at least one of removing the effects of leak-through of excitation-level light into the = ∗−(1: signal and removing the autofluorescence contribution to the = ∗−(1: signal; and monitoring the IF agent signal for each measurement data entry within the post-agent- administration n of the measurement data set.

2. The method of claim 1, wherein removing the effects of hrough of excitationlevel light into the = ∗−(1: signal comprises transforming each = signal into an ExLT ∗−(1 signal representing a level of excitation-wavelength light leak-through using Eqn. (21): : = = =−( ∗= = Eqn. (21) ∗−(1 where = =−( is a calibration factor.

3. The method of claim 2, wherein removing the effects of leak-through of excitationlevel light into the = ∗−(1: signal further comprises transforming each = ∗−(1: signal into a corrected fluorescence signal = ∗= ∗ =, representing detected emission-wavelength fluorescence only, using Eqn. (23): = ∗= ∗ = = = ∗−(1: −= ∗− Eqn. (23).

4. The method of claim 3, wherein ng the effects of autofluorescence comprises determining IF auto , enting intrinsic autofluorescence emitted by chromophores within the diffuse ting medium in addition to the fluorescent agent, by analyzing the = ∗= ∗ = signals obtained according to Eqn. (32): === −− = (1:= ∗(= ∗= ∗ =(1:(=(=(= ∗== ((== Eqn. (32), wherein (1:endBackground ) represents a portion of the measurement dataset ed prior to administration of the fluorescent agent.

5. The method of claim 4, wherein removing the effects of autofluorescence further comprises subtracting IF auto from = ∗= ∗ =to obtain IF agent .

6. The method of claim 2, wherein (1=−( is obtained by: ing ements from a solid phantom, the measurements comprising: a fluorescence signal = ∗−(1: representing emission-wavelength fluorescence measured using the filtered light detector; an excitation-wavelength light signal = = measured using the non-filtered ∗−(1 light detector; and computing = =−( according to Eqn. (22): ∗=−( = = ∗= ∗− Eqn. (22). = === ∗−

7. A method of determining renal on in a patient, the method comprising: providing a measurement data set comprising a plurality of measurement s, each measurement data entry comprising at least two measurements obtained at a corresponding data acquisition time from a tissue of a patient before and after administration of an exogenous fluorescent agent, the at least two measurements sing an = ∗−(1: signal detected at a third region adjacent to the diffuse reflecting medium by a filtered light detector during illumination of the diffuse reflecting medium by excitatory-wavelength light from the first region, and at least one DR signal selected from: a = signal detected at a second region adjacent to the diffuse reflecting ∗−(1 medium by an unfiltered light detector during illumination of the diffuse reflecting medium by excitatory-wavelength light from a first region adjacent to the diffuse reflecting medium; a = ∗−(1: signal detected at a third region adjacent to the diffuse reflecting medium by a filtered light detector during illumination of the diffuse ting medium by excitatory-wavelength light from the first region; a DR em signal ed at the second region by the unfiltered light detector during nation of the diffuse reflecting medium by emission-wavelength light from the first position; and a DR tered signal detected at the third region by the filtered light detector during illumination of the diffuse reflecting medium by emission-wavelength light from the first position; and; transforming each = ∗−(1: signal of each measurement data entry within the post-agentadministration portion of the measurement data set to an IF agent signal representing a detected fluorescence ity d solely by the fluorescent agent from within the diffuse reflecting medium, wherein transforming each = ∗−(1: signal ses at least one of removing the effects of leak-through of excitation-level light into the = ∗−(1: signal and removing the autofluorescence contribution to the = ∗−(1: signal; identifying a post-equilibration portion of the measurement data set; transforming the IF agent signals corresponding to the post-equilibration portion of the measurement data set to a rate of change of the IF agent signals; and determining the renal on in the patient based on the rate of change of the IF agent signals.

8. The method of claim 7, wherein removing the effects of leak-through of excitationlevel light into the = ∗−(1: signal ses transforming each = signal into an ExLT ∗−(1 signal representing a level of excitation-wavelength light leak-through using Eqn. (21): : = = =−( ∗= = Eqn. (21) ∗−(1 where = =−( is a calibration .

9. The method of claim 8, n removing the effects of leak-through of tionlevel light into the = ∗−(1: signal further comprises transforming each = ∗−(1: signal into a corrected fluorescence signal = ∗= ∗ =, representing detected emission-wavelength fluorescence only, using Eqn. (23): = ∗= ∗ = = = ∗−( −= ∗− Eqn. (23).

10. The method of claim 9, wherein removing the effects of autofluorescence comprises determining IF auto , representing intrinsic uorescence emitted by chromophores within the diffuse reflecting medium in addition to the fluorescent agent, by analyzing the = ∗= ∗ = signals obtained using the equation according to Eqn. (32): === −− = (1:= ∗(= ∗= ∗ =(1:(=(=(= ∗== ((== Eqn. (32), wherein (1:endBackground ) represents a portion of the measurement dataset obtained prior to administration of the fluorescent agent.

11. The method of claim 10, wherein removing the effects of autofluorescence further ses subtracting IF auto from = ∗= ∗ = to obtain IF agent .

12. The method of claim 8, wherein (1=−( is obtained by: ing measurements from a solid phantom, the measurements comprising: a fluorescence signal = ∗−(1: representing emission-wavelength fluorescence measured using the filtered light detector; an excitation-wavelength light signal = measured using the non-filtered ∗−(1 light detector; and computing = =−( according to Eqn. (22): ∗=−( = = ∗= ∗− Eqn. (22). = === ∗− WO 40984 113? SUBSTITUTE SHEET (RULE 26) 2131 \ QPERATEQN DESPLAY UNET UNET 212 ..... CONTRGLLER 232 ~ UNET CQNTRQL UNiT SGNTRDL UNET 2 ~ . . 222202 2222(2) 222 . ADDETEQNAL LEGHT TEMPERATURE DETECTOR(S) SENSQR(S) _ . FERST LEGHT FERST LEGHT SECQND LEGHT SECQNQ LEGHT EETECTQR SQURCE SGURCE BETECTOR SUBSTITUTE SHEET (RULE 26) WO 40984 3131 00 mmlflfl zommwfim ZMmOJwOmexwa ZOEflQOm/w Ea“ W GE % szmfixgg N/E M M “2 EMA ND “\ng Rummw .. SLENfl AHVELESHV SUBSTITUTE SHEET (RULE 26)