NZ747845B2 - Flowcell cartridge with floating seal bracket - Google Patents
Flowcell cartridge with floating seal bracket Download PDFInfo
- Publication number
- NZ747845B2 NZ747845B2 NZ747845A NZ74784517A NZ747845B2 NZ 747845 B2 NZ747845 B2 NZ 747845B2 NZ 747845 A NZ747845 A NZ 747845A NZ 74784517 A NZ74784517 A NZ 74784517A NZ 747845 B2 NZ747845 B2 NZ 747845B2
- Authority
- NZ
- New Zealand
- Prior art keywords
- support bracket
- microfluidic plate
- edge
- indexing feature
- microfluidic
- Prior art date
Links
- 230000000875 corresponding Effects 0.000 claims description 20
- 239000000126 substance Substances 0.000 abstract description 3
- 239000011521 glass Substances 0.000 description 67
- 238000005096 rolling process Methods 0.000 description 7
- 238000010586 diagram Methods 0.000 description 4
- 239000000203 mixture Substances 0.000 description 4
- 239000000463 material Substances 0.000 description 2
- 229920000160 (ribonucleotides)n+m Polymers 0.000 description 1
- NIXOWILDQLNWCW-UHFFFAOYSA-N acrylic acid group Chemical group C(C=C)(=O)O NIXOWILDQLNWCW-UHFFFAOYSA-N 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 229920003013 deoxyribonucleic acid Polymers 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 238000005755 formation reaction Methods 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 230000000116 mitigating Effects 0.000 description 1
- 239000002991 molded plastic Substances 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/02—Adapting objects or devices to another
- B01L2200/025—Align devices or objects to ensure defined positions relative to each other
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/02—Adapting objects or devices to another
- B01L2200/026—Fluid interfacing between devices or objects, e.g. connectors, inlet details
- B01L2200/027—Fluid interfacing between devices or objects, e.g. connectors, inlet details for microfluidic devices
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/04—Exchange or ejection of cartridges, containers or reservoirs
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/06—Fluid handling related problems
- B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/06—Fluid handling related problems
- B01L2200/0689—Sealing
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/04—Closures and closing means
- B01L2300/041—Connecting closures to device or container
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/06—Auxiliary integrated devices, integrated components
- B01L2300/0609—Holders integrated in container to position an object
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0809—Geometry, shape and general structure rectangular shaped
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0809—Geometry, shape and general structure rectangular shaped
- B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0809—Geometry, shape and general structure rectangular shaped
- B01L2300/0819—Microarrays; Biochips
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0809—Geometry, shape and general structure rectangular shaped
- B01L2300/0822—Slides
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0848—Specific forms of parts of containers
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
- B01L2300/0877—Flow chambers
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0887—Laminated structure
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502715—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L9/00—Supporting devices; Holding devices
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L9/00—Supporting devices; Holding devices
- B01L9/52—Supports specially adapted for flat sample carriers, e.g. for plates, slides, chips
- B01L9/527—Supports specially adapted for flat sample carriers, e.g. for plates, slides, chips for microfluidic devices, e.g. used for lab-on-a-chip
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
-
- F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
- F16—ENGINEERING ELEMENTS AND UNITS; GENERAL MEASURES FOR PRODUCING AND MAINTAINING EFFECTIVE FUNCTIONING OF MACHINES OR INSTALLATIONS; THERMAL INSULATION IN GENERAL
- F16K—VALVES; TAPS; COCKS; ACTUATING-FLOATS; DEVICES FOR VENTING OR AERATING
- F16K99/00—Subject matter not provided for in other groups of this subclass
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/00029—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor provided with flat sample substrates, e.g. slides
- G01N2035/00099—Characterised by type of test elements
- G01N2035/00158—Elements containing microarrays, i.e. "biochip"
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/00029—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor provided with flat sample substrates, e.g. slides
Abstract
cartridge for use with chemical or biological analysis systems is provided. The cartridge may include a floating microfluidic plate that is held in the cartridge using one or more floating support brackets that incorporate gaskets that may seal against fluidic ports on the microfluidic plate. The floating support brackets may include indexing features that may align the microfluidic plate with the seals. floating support brackets may include indexing features that may align the microfluidic plate with the seals.
Description
FLOWCELL CARTRIDGE WITH FLOATING SEAL BRACKET
CROSS-REFERENCE TO RELATED APPLICATION
This application claims benefit of priority to U.S. Patent Application No.
15/841,109, filed December 13, 2017, which claims benefit of priority to U.S. Patent
Application No. 62/441,927, filed January 3, 2017, and also claims benefit to British
(GB) Patent Application No. 1704769.7, filed March 24, 2017, which also claims
benefit of priority to U.S. Patent Application No. 62/441,927; all of these prior
applications are hereby incorporated by reference herein in their entireties.
BACKGROUND
Sequencers, e.g., genome sequencers, such as DNA sequencers or RNA
sequencers, and other biological or chemical analysis systems may sometimes utilize
microfluidic flowcells, such as may be provided by way of a glass plate having
microfluidic flow channels etched therein. Such flowcells may be made as a
laminated stack of layers, with the flow channels etched in one or more of the layers.
In most flowcells, access to the flow channels within the flowcell may be provided by
way of openings that pass through one or both of the outermost layers to reach the
flow channels within.
[0003] Since it is difficult to decontaminate a flowcell after a sample has been
flowed through it, it is common to replace the flowcell before analyzing a particular
sample. As such, it is common for flowcells to be implemented using a cartridge-
based approach to facilitate easy replacement of the flowcells.
SUMMARY
Details of one or more implementations of the subject matter described in
this specification are set forth in the accompanying drawings and the description
below. Other features, aspects, and advantages will become apparent from the
description, the drawings, and the claims. Note that the relative dimensions of the
following figures may not be drawn to scale unless specifically indicated as being
scaled drawings.
In some implementations, an apparatus is provided that includes a frame, a
microfluidic plate having one or more first fluidic ports in a first side, and a first
support bracket that is attached to the frame such that the microfluidic plate is
interposed between the first support bracket and the frame, the first support
bracket floats relative to the microfluidic plate and the frame, the microfluidic plate
and the frame float relative to one another, and a first side of the first support
bracket faces towards the microfluidic plate. In such implementations, the first
support bracket may include a first indexing feature that protrudes from the first
side of the first support bracket and is proximate to a first edge of the microfluidic
plate and may also include a second indexing feature that protrudes from the first
side of the first support bracket and is proximate to a second edge of the microfluidic
plate. The first support bracket may include a first gasket with at least one seal that
is proud of the first side of the first support bracket and is positioned against the first
side of the microfluidic plate, and the first indexing feature of the first support
bracket and the second indexing feature of the first support bracket may contact the
first edge and the second edge, respectively, of the microfluidic plate when the at
least one seal of the first gasket is aligned with a corresponding at least one of the
one or more first fluidic ports.
In some such implementations, the microfluidic plate may have a second side
opposite the first side, the frame may have a first overlapping portion that overlaps,
when viewed along a direction perpendicular to a major surface of the microfluidic
plate, a first portion of the microfluidic plate that includes the second edge, the first
overlapping portion may be proximate to the second side of the microfluidic plate,
the first overlapping portion may have a first clamp arm slot having a first slot width
in a direction parallel to the second edge, the second side of the microfluidic plate
may be visible, e.g., to the unaided eye, through the first clamp arm slot, the
apparatus may be to, or configured to be, interfaced with a receiver of an analysis
device, the receiver having a first clamp arm that is movable from an unclamped
position in which the first clamp arm does not press on the second side of the
microfluidic plate and does not engage with the first clamp arm slot to a clamped
position in which the first clamp arm presses on the second side of the microfluidic
plate and engages with the first clamp arm slot, and the first slot width may be larger
than a width of the first clamp arm in a direction parallel to the second edge and
located within the first clamp arm slot when the first clamp arm is in the clamped
position.
In some such implementations of the apparatus, the microfluidic plate may
have a third edge opposite the first edge and a fourth edge opposite the second
edge, the frame may have a second overlapping portion that overlaps, when viewed
along the direction perpendicular to the major surface of the microfluidic plate, a
second portion of the microfluidic plate that includes the fourth edge, the second
overlapping portion may be proximate to the second side of the microfluidic plate,
and the second overlapping portion may have a second clamp arm slot having a
second slot width in a direction parallel to the fourth edge, the second side of the
microfluidic plate may be visible through the second clamp arm slot, the receiver of
the analysis device within which the apparatus is to be, or configured to be,
interfaced may have a second clamp arm that is movable from an unclamped
position in which the second clamp arm does not press on the second side of the
microfluidic plate and does not engage with the second clamp arm slot to a clamped
position in which the second clamp arm presses on the second side of the
microfluidic plate and engages with the second clamp arm slot, and the second slot
width may be larger than a width of the second clamp arm in a direction parallel to
the fourth edge and located within the second clamp arm slot when the second
clamp arm is in the clamped position.
In some implementations of the apparatus, there may be two first fluidic
ports in the microfluidic plate, and the first gasket may include two seals, each seal
having a through-hole passing through the first support bracket and aligned with a
different one of the first fluidic ports when the first indexing feature of the first
support bracket and the second indexing feature of the first support bracket contact
the first edge and the second edge, respectively, of the microfluidic plate.
In some such implementations, the first gasket may include a support foot
that is proud of the first side of the first support bracket and is positioned against the
microfluidic plate, a first axis may be defined between center points of the two seals
of the first gasket, the support foot of the first gasket may be offset by a first amount
from the first axis along a second axis perpendicular to the first axis and parallel to
the microfluidic plate, and the support foot of the first gasket may have an upper
surface that contacts the microfluidic plate and is co-planar with upper surfaces of
the two seals of the first gasket that are also in contact with the microfluidic plate.
In some further such implementations of the apparatus, the support foot of the first
gasket may not serve as a seal.
In some implementations of the apparatus, the first gasket may be co-
molded into the first support bracket.
In some implementations of the apparatus, the first support bracket may
have a second side that faces away from the first side of the first support bracket,
and at least two first fluidic port indexing features may protrude from the second
side of the first support bracket, each first fluidic port indexing feature to, or
configured to, engage with a corresponding fluidic port indexing hole on a first fluidic
port block of an analysis device to, or configured to, receive the apparatus.
In some implementations of the apparatus, the frame may include two
opposing first retaining clips with opposing surfaces that face one another, the first
support bracket may be positioned in between the two opposing first retaining clips,
the opposing surfaces of the first retaining clips may be spaced apart by a first
distance, and a portion of the first support bracket between the opposing surfaces of
the first retaining clips may have a first width in a direction spanning between the
opposing surfaces of the first retaining clips that is less than the first distance.
In some implementations of the apparatus, the first support bracket may
include a third indexing feature that protrudes from the first side of the first support
bracket and is proximate to a third edge of the microfluidic plate opposite the first
edge of the microfluidic plate, and the microfluidic plate may be interposed between
the first indexing feature of the first support bracket and the third indexing feature
of the first support bracket.
In some implementations of the apparatus, the microfluidic plate may be
rectangular and the first edge of the microfluidic plate may be orthogonal to the
second edge of the microfluidic plate and the second edge of the microfluidic plate
may be orthogonal to the third edge of the microfluidic plate.
In some implementations of the apparatus, the frame may have a
substantially rectangular opening, the microfluidic plate may sit within the
substantially rectangular opening, the substantially rectangular opening may have
opposing side walls that face towards one another, and the first indexing feature of
the first support bracket may be interposed between one of the opposing side walls
of the substantially rectangular opening and the first edge of the microfluidic plate
and the third indexing feature of the first support bracket may be interposed
between the other opposing side wall of the opposing side walls of the substantially
rectangular opening and the third edge of the microfluidic plate.
In some implementations of the apparatus, the substantially rectangular
opening may have an opening width in a direction parallel to the second edge, a first
indexing feature width may exist between furthest-apart portions of the surfaces of
the first indexing feature of the first support bracket and the third indexing feature
of the first support bracket that face the opposing side walls of the substantially
rectangular opening, and the opening width minus the first indexing feature width
may be less than the first distance minus the first width.
In some implementations, the microfluidic plate may further include one or
more second fluidic ports on the first side and the apparatus may further include a
second support bracket that is attached to the frame such that the microfluidic plate
is interposed between the second support bracket and the frame, the second
support bracket floats relative to the microfluidic plate and the frame, the
microfluidic plate and the frame float relative to one another, and a first side of the
second support bracket faces towards the microfluidic plate. In such
implementations, the second support bracket may include a first indexing feature
that protrudes from the first side of the second support bracket and is proximate to
the first edge of the microfluidic plate, the second support bracket may include a
second indexing feature that protrudes from the first side of the second support
bracket and is proximate to a fourth edge of the microfluidic plate opposite the
second edge of the microfluidic plate, the microfluidic plate may be interposed
between the second indexing feature of the first support bracket and the second
indexing feature of the second support bracket, the second support bracket may
include a second gasket with at least one seal that is proud of the first side of the
second support bracket and is positioned against the microfluidic plate, and the first
indexing feature of the second support bracket and the second indexing feature of
the second support bracket may contact the first edge and the fourth edge,
respectively, of the microfluidic plate when the at least one seal of the second gasket
is aligned with a corresponding at least one of the one or more second fluidic ports.
[0018] In some such implementations, the frame may include two opposing second
retaining clips with opposing surfaces that face one another, the second support
bracket may be positioned in between the two opposing second retaining clips, the
opposing surfaces of the second retaining clips may be spaced apart by a second
distance, and a portion of the second support bracket between the opposing
surfaces of the second retaining clips may have a second width in a direction
spanning between the opposing surfaces of the second retaining clips that is less
than the second distance.
In some further such implementations, the second support bracket may
include a third indexing feature that protrudes from the first side of the second
support bracket and is proximate to the third edge of the microfluidic plate, and the
microfluidic plate may be interposed between the first indexing feature of the
second support bracket and the third indexing feature of the second support
bracket.
In some additional such implementations, the frame may have a substantially
rectangular opening, the microfluidic plate may have a third edge opposite the first
edge, the microfluidic plate may sit within the substantially rectangular opening, the
substantially rectangular opening may have opposing side walls that face towards
one another and that define an opening width in a direction parallel to the second
edge, the first indexing feature of the second support bracket may be interposed
between one of the opposing side walls of the substantially rectangular opening and
the first edge of the microfluidic plate and the third indexing feature of the second
support bracket may be interposed between the other opposing side wall of the
opposing side walls of the substantially rectangular opening and the third edge of
the microfluidic plate, the microfluidic plate may have a plate width in a direction
spanning between the first indexing feature of the second support bracket and the
third indexing feature of the second support bracket, a second indexing feature
width may exist between furthest-apart portions of the surfaces of the first indexing
feature of the second support bracket and the third indexing feature of the second
support bracket that face the opposing side walls of the substantially rectangular
opening, and the opening width minus the second indexing feature width may be
less than the second distance minus the second width.
In some implementations, there may be two second fluidic ports in the
microfluidic plate, and the second gasket may include two seals, each seal having a
through-hole passing through the second support bracket and aligned with a
different one of the second fluidic ports when the first indexing feature of the
second support bracket and the second indexing feature of the second support
bracket contact the first edge and the fourth edge, respectively, of the microfluidic
plate.
In some implementations, the second gasket may include a support foot that
is proud of the first side of the second support bracket and is positioned against the
microfluidic plate, a third axis may be defined between center points of the two
seals of the second gasket, the support foot of the second gasket may be offset by a
second amount from the third axis along a fourth axis perpendicular to the third axis
and parallel to the microfluidic plate, and the support foot of the second gasket may
have an upper surface that contacts the microfluidic plate and may be co-planar with
upper surfaces of the two seals of the second gasket that are also in contact with the
microfluidic plate. In some such implementations, the support foot of the second
gasket may not serve as a seal. In some alternative or additional such
implementations, the second gasket may be co-molded into the second support
bracket.
[0023] In some implementations, the second support bracket may have a second
side that faces away from the first side of the second support bracket, and at least
two second fluidic port indexing features may protrude from the second side of the
first support bracket, each first fluidic port indexing feature to, or configured to,
engage with a corresponding fluidic port indexing hole on a first fluidic port block of
an analysis device to, or configured to, receive the apparatus.
These and other implementations are described in further detail with
reference to the Figures and the detailed description below. Other features, aspects,
and advantages will become apparent from the description, the drawings, and the
claims. Note that the relative dimensions of the following figures may not be drawn
to scale.
BRIEF DESCRIPTION OF THE DRAWINGS
The various implementations disclosed herein are illustrated by way of
example, and not by way of limitation, in the figures of the accompanying drawings,
in which like reference numerals refer to similar elements.
Figure 1 depicts an exploded isometric view of an example flowcell cartridge.
Figure 2 depicts an exploded underside isometric view of the example
flowcell cartridge of Figure 1.
Figure 3 depicts a front isometric view of the example flowcell cartridge of
Figure 1 in an unexploded state.
Figure 4 depicts a rear isometric view of the example flowcell cartridge of
Figure 1 in an unexploded state.
Figures 5 and 6 are diagrams illustrating how a seal can roll when the surfaces
between which the seal is interposed are translated laterally.
Figures 7 and 8 are diagrams illustrating how a gasket with a support foot can
prevent the rolling behavior illustrated in Figures 5 and 6.
[0032] Figure 9 depicts an isometric view of a floating support bracket of the
example flowcell cartridge of Figure 1.
Figure 10 depicts an underside isometric view of the floating support bracket
of the example flowcell cartridge of Figure 1.
Figure 11 depicts an isometric view of an example receiver for the example
flowcell cartridge of Figure 1.
Figure 12 depicts an exploded isometric view of the example receive of Figure
11 and the example flowcell cartridge of Figure 1.
Figure 13 depicts a plan view of the example flowcell cartridge of Figure 1.
Figures 14 through 17 depict various stages of component alignment that
may occur during clamping of an example flowcell cartridge.
Figures 1 through 4 and 9 through 13 are drawn to scale within each Figure,
although the scale of the depicted embodiments may vary from Figure to Figure.
DETAILED DESCRIPTION
[0039] The present inventors have conceived of new designs for a flowcell cartridge,
such as may be used in chemical and biological analysis systems that utilize
microfluidic flow structures contained within a glass plate structure. These concepts
are discussed herein with respect to the following Figures, although it will be
appreciated that these concepts may be implemented in cartridge designs other
than the specific example shown, and that such other implementations would still
potentially fall within the scope of the claims.
Figure 1 depicts an exploded isometric view of an example flowcell cartridge.
In Figure 1, the flowcell cartridge 100 has a frame 102 that may, for example, be
made of molded plastic or other, durable material. The frame may provide a support
structure for supporting a glass plate (or a plate of other material, e.g., acrylic or
other plastic), such as glass plate 114 that contains microfluidic flow structures; this
plate may also be referred to herein as a microfluidic plate. In this example, the
glass plate, which has a first edge 122, a second edge 124, a third edge 126, and a
fourth edge 128, includes four sets of multiple, parallel microfluidic flow channels
that extend along directions parallel to the long axis of the glass plate, e.g., along
axes that are parallel to the first edge 122 and/or the third edge 126. To the extent
applicable, the terms “first,” “second,” “third,” etc. (or other ordinal indicators)
herein are merely employed to show the respective objects described by these
terms as separate entities and are not meant to connote a sense of chronological
order, unless stated explicitly otherwise herein. The first edge 122 and the third
edge 126 may be generally orthogonal to the second edge 124 and the fourth edge
128 in some implementations, but may be other orientations in other
implementations. As can be seen in Figure 2, which depicts an exploded underside
isometric view of the example flowcell cartridge of Figure 1, each set of microfluidic
flow structures may terminate in one or more first fluidic ports 118 and one or more
second fluidic ports 120. The first and second fluidic ports 118 and 120 may be
located in a first side 116 of the glass plate 114, although other implementations
may only include the first fluidic ports 118 or the second fluidic ports 120 on the first
side 116. The frame 102 may have a substantially rectangular opening (or opening of
another shape) 104 that is sized to receive the glass plate 114; the rectangular
opening 104 may include opposing side walls 106 that are in close proximity to the
first edge 122 and the third edge 126 of the glass plate 114 when the cartridge is
fully assembled. As used herein, the term “substantially rectangular” is use to refer
to an opening that has an overall rectangular shape, although there may be various
features or discontinuities in the overall shape, such as the semi-circular notches
along one side wall of the depicted rectangular opening, or the clamp arm slots
along the short edges of the rectangular opening 104. The opposing side walls 106
may be spaced apart by an opening width 195 to allow the first support bracket 132
and the second support bracket 160, and thus the glass plate 114, to float within the
rectangular opening 104 for at least some range of movement, e.g., about 1mm to
about 2mm or less.
The glass plate 114 may be held in place in the cartridge 100 through the use
of one or more support brackets, such as a first support bracket 132 and a second
support bracket 160. In this discussion, only the features of the first support bracket
132 are discussed in detail, although it is readily apparent from the Figures that the
second support bracket 160, which may or may not be identical to the first support
bracket 132, is at least structurally similar to the first support bracket 132 and may
operate in a similar manner.
The first support bracket 132 may have a first side 134 (see Figure 1) and a
second side 136 (see Figure 2). The first side 134 may face towards the glass plate
114 and may have a first indexing feature 138, e.g., a molded pin or post, that
extends away from the first side 134 and that is at least long enough that the side of
the first indexing feature 138 that faces towards the glass plate 114 may contact the
glass plate 114 when the cartridge is fully assembled. The first indexing feature 138
may be positioned on the first support bracket 132 such that the first indexing
feature 138 is proximate to, or contacting, the first edge 122 of the glass plate 114
when the cartridge is fully assembled. The first support bracket 132 may also have
one or more second indexing features 140 (an additional second indexing feature
140' is also shown in Figure 1) that may be similar to the first indexing feature 138
except that each second indexing feature 140 may be positioned on the first support
bracket 132 such that the second indexing feature 140 is proximate to, or physically
contacts, the second edge 124 of the glass plate 114. The first support bracket 132
may also include a third indexing feature 142, which may be positioned on an
opposite end of the first support bracket 132 from the first indexing feature 138.
The first indexing feature 138 and the third indexing feature 142, if used, may be
separated from one another by a first float gap 156, which may be sized to be slightly
larger than the plate width 130 so as to allow the glass plate 114 to “float” within the
confines of the first indexing feature 138 and the third indexing feature 142. The
furthest-apart surfaces of the first indexing feature 138 and the third indexing
feature 142 may similarly define a first indexing feature width 157. The opening
width 195 may be wider than the first indexing feature width 157 so that the first
support bracket 132 may float laterally between the opposing side walls 106 of the
rectangular opening 104.
[0043] The first support bracket may also include one or more first gaskets 144,
which may include one or more seals 146 (each first gasket 144, in this example,
includes two seals 146, each positioned so as to interface with a different first fluidic
port 118). The first gaskets 144 may, for example, be insertable into the first support
bracket 132 or may, in some implementations, be co-molded with the first support
bracket 132 (in the latter case, the first gaskets 144 and the first support bracket 132
may, in effect, be treated as a single component). The seals may be proud of the
first side 134 and, optionally, the second side 136 of the first support bracket so that
they may compress against the glass plate 114 and, as discussed later herein, a
fluidic port block, respectively. In some implementations, the seal may not be proud
of the second side 136 of the first support bracket, e.g., if the fluidic port block that
faces the second side 136 when the cartridge is installed in an analysis device has a
raised boss that may engage with the seal.
The first gasket 144 may also include a support foot 148, which may be
provided to prevent or mitigate “rolling” of the first gasket 144 about an axis passing
through the centers of the seals 146 when the first support bracket 132 is translated
in a direction parallel to the major surface of the glass plate 114 while the seals 146
are in contact with the glass plate 114. To this end, the support foot 148 may be
offset from a first axis 150 spanning between the centers of the seals 146 of the first
gasket 144 along a second axis 152 perpendicular to the first axis 150 by some
amount so as to provide a moment arm to resist such rolling behavior. The support
foot 148 and the seals 146 may all be designed to have contact surfaces that contact
the glass plate 114 in concert when the glass plate 114 is brought into contact with
the first gasket 144. These contact surfaces may all be parallel to one another to
ensure that when the contact surface of the support foot 148 is in contact with the
glass plate 114, the contact surface(s) of the seal(s) 146 are also in good, i.e., not
having any misalignment gaps, contact with the glass plate 114. In the example
cartridge shown, each support bracket includes two first gaskets, although they may
be referred to as second gaskets, third gaskets, etc., in the interests of reducing
confusion, if needed. It is also be understood that the support foot 148, while
appearing similar to the seals 146, may actually not provide any “sealing”
characteristics at all—it may be present solely for the purposes of preventing or
mitigating “rolling.”
Figures 5 and 6 are diagrams illustrating how a seal can roll when the surfaces
between which the seal is interposed are translated laterally. In Figure 5, a glass
plate 514 is offset from a fluidic port block 564, and a support bracket 532 with a
gasket 544 is interposed between them. The gasket 544 has a seal 546 that is
aligned with a fluidic port 518’ in the fluidic port block 564, but that is misaligned
somewhat with a fluidic port 518 in the glass plate 514. As can be seen in Figure 6,
when the glass plate 514 is slid sideways so that the fluidic port 518 is aligned with
the seal 546, friction between the seal 546 and the glass plate 514/fluidic port block
564 may cause the seal 546 to not slide a commensurate distance—as a result, the
gasket 544 and the support bracket 532 may tilt or roll slightly, resulting in gaps 594
appearing between the seal 546 and the glass plate 514/fluidic port block 564. This
is, of course, undesirable, as it causes leakage.
Figures 7 and 8 are diagrams illustrating how a gasket with a support foot can
prevent the rolling behavior illustrated in Figures 5 and 6. As can be seen, the gasket
544 has been extended to the right and a support foot 748 has been added to the
gasket 544. When the glass plate 514 is slid to the left, as in Figure 6, the support
foot 748 introduces a counter-moment to any potential rolling moment caused by
friction between the seal 546 and the glass plate 514/fluidic port block 564. This
prevents the formation of the gaps 594 and keeps the seal 546 in good contact with
the surfaces it seals.
The first support bracket 132 may snap into two opposing first retaining clips
108 (only one is visible in Figure 2, as the other is obscured by other features of the
frame 102—however, there are corresponding second retaining clips visible on the
opposite end of the frame 102 that are configured similarly but at a different
location). The first retaining clips 108 may have opposing surfaces 110 that are
separated from one another by a first distance 112. The first distance may be
greater than a first width 158 of the first support bracket 132, thereby allowing the
first support bracket 132 to float laterally by a small amount when snapped into the
first retaining clips 108. In some implementations, the amount of float between the
first support bracket 132 and the opposing side walls 106, i.e., the opening width 195
minus the first indexing feature width 157, may be smaller than the amount of float
between the first support bracket 132 and the retaining clips 108, i.e., the first
distance 112 minus the first width 158. Similar relationships may exist for the
second support bracket 160.
Figure 3 depicts a front isometric view of the example flowcell cartridge of
Figure 1 in an unexploded/assembled state. Figure 4 depicts a rear isometric view of
the example flowcell cartridge of Figure 1 in an unexploded/assembled state. As can
be seen, the glass plate 114 is held in place within the frame 102 by the first support
bracket 132 and the second support bracket 160, which, in turn, are held in place by
the first retaining clips 108 and second retaining clips, respectively. The frame may
have a first overlapping portion 196 and a second overlapping portion 196' (see
Figure 2) that overlap with a corresponding first portion 197 and second portion 197'
(see Figure 1) of the glass plate 114. The first portion 197 may include the second
edge 124, and the second portion 197' may include the fourth edge 128. The
overlapping portions 196/196' may prevent the glass plate 114 from falling out of
the front of the frame 102, e.g., the glass plate 114 may be sandwiched between the
overlapping portions 196/196' and the first/second support brackets 132/160. The
glass plate 114 may still, however, be free to float within the frame to some degree.
[0049] Figure 9 depicts an isometric view of the first support bracket 132 of the
example flowcell cartridge 100 of Figure 1. Figure 10 depicts an underside isometric
view of the first support bracket 132 of the example flowcell cartridge 100 of Figure
1. In addition to the first indexing feature 138, the second indexing feature(s) 140,
and possibly the third indexing feature 142, the first support bracket 132 may also
include first fluidic port indexing features 154 on the second side 136 of the first
support bracket 132 (the second support bracket 160 may have corresponding
second fluidic port indexing features as well). As can be seen, the first support
bracket has portions that extend beyond the first width 158, e.g., the small “teeth”
that are located at the four outermost corners of the first support bracket 132.
These teeth may engage with the first retaining clips 108 and may allow the first
support bracket 132 to also float along an axis parallel to the first edge 122 by some
limited amount.
In this example cartridge, the glass plate 114 may float with respect to the
support brackets 132 and 160, and the support brackets 132 and 160, in turn, may
float with respect to the frame 102. Thus, there are two tiers of floating components
in the example cartridge. The combination of these different tiers of floating
components, as well as the various indexing features provided, allow for the glass
plate 114 and the seals 146 to be properly aligned with each other and with ports on
floating manifold blocks located on equipment that receives the cartridge 100.
Figure 11 depicts an isometric view of an example receiver for the example
flowcell cartridge of Figure 1. As seen in Figure 11, a receiver 162 may be provided;
the receiver may be a subcomponent of a larger analysis device that utilizes the
cartridge 100. The receiver 162 may include a chuck 176, against which the glass
plate 114 may be drawn, e.g., by a vacuum, during analysis operations. The receiver
162, in this example, may include a pair of first fluidic port blocks 164 and an
opposing pair of second fluidic port blocks 166. The first fluidic port blocks 164 and
the second fluidic port blocks 166 may be configured to float slightly in directions at
least parallel to the upper surface of the chuck 176 (and possibly also in directions
perpendicular to the upper surface of the chuck 176). The ends of the receiver 162
may include, for example, a clamping mechanism that may serve to clamp the glass
plate 114 against the chuck 176. Such clamping mechanisms may, for example, have
clamp arms 172 that may rotate downwards and contact the upper surface of the
glass plate 114 of the cartridge 100 when the cartridge 100 is installed. The receiver
162 may also include indexing features that are located so as to engage with the
support brackets and glass plate 114 of the cartridge 100 when the cartridge 100 is
installed. For example, lateral indexing pins 168 may be placed such that the glass
plate 114 contacts the lateral indexing pins 168 when the glass plate 114 is
translated laterally along the short axis of the chuck 176, and longitudinal indexing
pins 170 may be positioned so as to contact the support brackets of the cartridge
100 when, for example, one of the longitudinal indexing pins 170 is moved towards
the other longitudinal indexing pins 170. In this example, the longitudinal indexing
pin 170 on the left is fixed in space relative to the receiver 162, whereas the other
longitudinal indexing pin 170 is configured to slide along an axis parallel to the long
axis of the chuck 176. The sliding longitudinal indexing pin 170 may be sprung so as
to be biased towards the other longitudinal indexing pin 170. The interaction of the
various indexing features is explained in more detail below, with respect to Figure
12.
Figure 12 depicts an exploded isometric view of the example receiver of
Figure 11 and the example flowcell cartridge of Figure 1. In this example, the
cartridge 100 has been shown in an exploded view, although the various
components that form the cartridge would be fully assembled, per Figure 3, prior to
the cartridge 100 being placed in the receiver 162.
When the cartridge 100 is laid on top of the receiver 162, the clamp arms 172
may rotate downward and engage with the top side of the glass plate 114. The
clamp arms 172 may also, as they pivot, translate along their rotational axes towards
the lateral indexing pins 168 such that the sides of the clamp arms 172 engage with
the sides of the rectangular notches or clamp arm slots 198, thereby causing the
entire frame 102 to translate along the same axis as well. For example, the clamp
arm slots 198 may be sized, e.g., with clamp arm widths 173 in a direction parallel to
the second edge 124 that are less than the widths of the clamp arm slots 198 in the
same direction, to allow the clamp arms 172 to swing through the clamp arm slots
198 freely and, during lateral translation of the clamp arms 172, press against the
sides of the clamp arm slots 198 facing away from the lateral indexing pins 168,
thereby pushing the frame 102 towards the lateral indexing pins 168. During this
lateral sliding motion, the frame 102 will (if not already in such a state) come into
contact with the first indexing feature 138 on the first support bracket 132 (and a
corresponding first indexing feature on the second support bracket 160) at indexing
feature contact points 182 located along one of the opposing side walls 106. As the
frame 102 continues to be translated towards the lateral indexing pins 168, the glass
plate 114 will eventually come into contact with both the lateral indexing pins 168
and the first indexing features 138 (see lateral indexing pin contact points 184 and
the indexing feature contact points 182 along the first edge 122 of the glass plate
114). Eventually, the first indexing features 138 will be sandwiched between the
frame 102 and the glass plate 114 (which is pressed against the lateral indexing pins
168), thereby locating the first support bracket 132 and the second support bracket
160 firmly in space in the lateral direction, i.e., perpendicular to the long axis of the
chuck 176. This aligns the seals on the first support bracket 132 and the second
support bracket 160 with the corresponding first fluidic ports 118 and the
corresponding second fluidic ports 120, respectively, on the glass plate 114.
Subsequent to, after, or in concert with the translation of the frame 102
towards the lateral indexing pins 168, the longitudinal indexing pins 170 may be
caused to move towards one another (one or both may move), thereby contacting
the facing edges of the first support bracket 132 and the second support bracket 160
and pushing the first support bracket ##32 and the second support bracket 160
towards one another. As the first support bracket ##32 and the second support
bracket 160 move towards one another, the glass plate 114 may come into contact
with the second indexing features 140 (and 140', if present) on the first support
bracket 132 and the second support bracket 160. The first support bracket 132 and
the second support bracket 160 may thus become aligned with the glass plate 114
and, consequently, the first fluidic ports 118 and the second fluidic ports 120.
After or during such plate alignment, the fluidic port blocks 164, 166 may be
raised so that the first fluidic port indexing features 154 (and corresponding second
fluidic port indexing features on the second support bracket 160) may be inserted
into corresponding alignment holes 188 on the first fluidic port block 164 and the
second fluidic port block 166. As the fluidic port block rises, the first fluidic port
indexing features 154 and the second fluidic port indexing features may engage with
the corresponding alignment holes 188 and force the first fluidic port blocks 164 and
the second fluidic port blocks 166 into alignment with the first support bracket 132
and the second support bracket 160, respectively. This, in turn, ensures that the
corresponding seals 146 on the respective support brackets 132, 160 line up with the
fluidic ports on the first fluidic port blocks 164 and the second fluidic port blocks 166,
respectively.
Thus, the cartridge 100 may have multiple levels of floating components that
engage with different sets of indexing features/pins in the cartridge 100 and located
on the receiver 162 and are moved into precisely aligned positions that cause the
fluidic ports, seals, and port block ports to line up, e.g., such that the centerlines of
the fluidic ports, seals, and port block ports are, in some implementations, within
less than about 0.05mm of one another, thereby ensuring a high-quality liquid-tight
seal. At the same time, some implementations of the cartridge may feature
additional features in the floating brackets, e.g., support feet, that may prevent
rolling behavior of the seal, thereby ensuring the integrity of any sealed connections.
Some of the floating components, e.g., the support brackets, may also act to retain
other floating components, e.g., the glass plate, in a manner that prevents stressing
the glass plate due to thermal expansion mismatches between the glass plate and
the cartridge frame, minor flexure of the cartridge frame, and so forth.
The floating behavior of the various components in the cartridge 100 may be
better understood with reference to Figure 13, which depicts a plan view of the
example flowcell cartridge of Figure 1. For reference purposes, the lateral indexing
pins 168 are shown as dotted circles and the outlines of the clamp arms 172 are
shown as dotted, rounded rectangles, but the remainder of the components shown
are part of the cartridge 100. The clamp arms 172 are shown in both an “engaged”
position (black line font) in which they are engaged with and pressed against the
sides of the clamp arm slots 198 (see Figure 2) and a non-engaged position (grey line
font), which may be their position prior to translating laterally. The glass plate 114
may1be able to move laterally by an amount relative to the frame 102 that is limited
by the first and second indexing features 138 and 142, respectively11. The first and
second support brackets may be able to move laterally (as well as longitudinally) by a
lesser amount, as is shown by the bracket float envelopes 180. For example, the first
and second support brackets may be able to float laterally by a distance of X, which
may be the opening width 195 minus the first indexing feature width 157, relative to
the frame, and the glass plate 114 may be able to float laterally by a distance of Y,
which may be the first float gap 156 minus the plate width 130, relative to the first
and second support brackets 132 and 160. In some such implementations, Y may be
less than X—however, the glass plate 114 may still float by a larger amount relative
to the frame 102 than the first and second support brackets 132 and 160 since the
glass plate 114 has a total overall float relative to the frame 102 of X+Y. This may
allow for considerable adjustment in the positioning of the glass plate.
An example alignment sequence is reviewed in Figures 14 through 17, which
depict various stages of component alignment that may occur during clamping of an
example flowcell cartridge. In Figure 14, the frame 1402 (shown in solid lines) of a
flowcell cartridge is lowered onto a receiver with two floating fluidic port blocks
1464 (shown in dashed lines). As can be seen, the fluidic port blocks 1464 are
slightly askew due to the fact that both are “floating.” Also visible in Figure 14 is the
outline of a support bracket 1432 (dotted lines) and a glass plate 1414 (dash-dot-
dash lines). There are four instances of fluidic ports 1418 across the glass plate 1414.
As can be seen, at each fluidic port 1418, there are corresponding features belonging
to the support bracket (dotted circles) and fluidic port blocks (dashed lines). These
correspond, for example, to the holes in the seals 146 and to the ports in the fluidic
port blocks 1464. As is evident, there is some alignment between these three
separate fluidic flow features at each location, but the alignment is far from ideal,
resulting in differently-configured apertures at each location which may cause
imbalances in fluid flow.
In Figure 15, the support bracket 1432 has been fully engaged with the fluidic
port blocks 1464 so that fluidic port indexing features 1454 (see Figure 14) are fully
inserted into alignment holes 1488 (also see Figure 14). The alignment holes 1488,
for example, may be countersunk and the fluidic port indexing features 1454 may
have conical or rounded tips so that they may engage with one another even if
somewhat misaligned; as the fluidic port indexing features 1454 are more fully
engaged with the alignment holes 1488, the countersink portion may narrow and
force the fluidic port indexing features 1454 to move towards the center of the
alignment holes 1488. As can be seen, one of the alignment holes 1488 for a given
fluidic port block 1464 may be circular, thereby providing both X and Y location
constraints, whereas the other may be obround to provide a single degree of
constraint, e.g., along only the Y axis, as this may be all that is needed in one
implementation to prevent rotation about the other alignment hole 1488. It is to be
recognized that the alignment holes 1488 and the fluidic port indexing features 1454
may also be swapped, i.e., the alignment holes 1488 may be located on the support
bracket 1432, and the fluidic port indexing features 1454 may be located on the
fluidic port block 1464.
Returning to Figure 15, the interfacing of the cartridge with the fluidic
support blocks 1464 causes the fluidic port blocks 1464 to come into alignment with
each other as well as with the support bracket 1432. Consequently, the ports on the
fluidic port blocks 1464 are now precisely aligned with the holes, e.g., the seals, on
the support bracket 1432. However, the holes/seals on the support bracket 1432
are not yet aligned with the fluidic ports 1418 on the glass plate.
[0061] In Figure 16, the glass plate 1414 has been moved upwards to contact second
indexing features 1440 on the support bracket 1432; this contact and the upward
movement of the glass plate 1414 causes the support bracket 1432 to move upwards
until it contacts longitudinal indexing pin 1470, thus firmly locking the support
bracket 1432 in place in the vertical direction (with respect to the Figure orientation;
in reality, this is more accurately called the longitudinal direction)—this aligns the
fluidic ports 1418 in the glass plate 1414 with the corresponding holes/seals in the
support bracket 1432 in the vertical direction.
Finally, in Figure 17, the frame 1402 may be pushed towards the lateral
indexing pin 1468. This causes the inside edge of the frame 1402 to contact first
indexing feature 1438, which causes the support bracket 1432, in turn, to move
towards the lateral indexing pin 1468 until the first indexing feature 1438 also
contacts the glass plate 1414 and pushes the opposite side of the glass plate 1414
into contact with the lateral indexing pin 1468. As can be seen, the first fluidic ports
1418 and the respective seal holes and fluidic port block holes are completely
aligned, thereby ensuring a consistently-sized flow aperture and proper seal
alignment.
The term “about” used throughout this disclosure, including the claims, is
used to describe and account for small fluctuations, such as due to variations in
processing. For example, unless otherwise specified herein in a particular context,
they can refer to less than or equal to ±5%, of the specified value or value equivalent
to the specified relationship, such as less than or equal to ±2%, such as less than or
equal to ±1%, such as less than or equal to ±0.5%, such as less than or equal to
±0.2%, such as less than or equal to ±0.1%, such as less than or equal to ±0.05%.
[0064] As noted earlier, any use of ordinal indicators, e.g., (a), (b), (c)… or the like, in
this disclosure and claims is to be understood as not conveying any particular order
or sequence, except to the extent that such an order or sequence is explicitly
indicated. For example, if there are three steps labeled (i), (ii), and (iii), it is to be
understood that these steps may be performed in any order (or even concurrently, if
not otherwise contraindicated) unless indicated otherwise. For example, if step (ii)
involves the handling of an element that is created in step (i), then step (ii) may be
viewed as happening at some point after step (i). Similarly, if step (i) involves the
handling of an element that is created in step (ii), the reverse is to be understood.
It is also to be understood that the use of "to," e.g., "the apparatus is to be
interfaced with a receiver of an analysis device," may be replaceable with language
such as "configured to," e.g., "the apparatus is configured to be interfaced with a
receiver of an analysis device", or the like.
It should be appreciated that all combinations of the foregoing concepts
(provided such concepts are not mutually inconsistent) are contemplated as being
part of the inventive subject matter disclosed herein. In particular, all combinations
of claimed subject matter appearing at the end of this disclosure are contemplated
as being part of the inventive subject matter disclosed herein. For the sake of
brevity, many of those permutations and combinations will not be discussed and/or
illustrated separately herein.
Claims (22)
1. An apparatus comprising: 5 a frame; a microfluidic plate having one or more first fluidic ports in a first side; and a first support bracket that is attached to the frame such that: the microfluidic plate is interposed between the first support bracket and the frame, 10 the first support bracket floats relative to the microfluidic plate and the frame, the microfluidic plate and the frame float relative to one another, and a first side of the first support bracket faces towards the microfluidic plate, wherein: 15 the first support bracket includes a first indexing feature that protrudes from the first side of the first support bracket and is proximate to a first edge of the microfluidic plate, the first support bracket includes a second indexing feature that protrudes from the first side of the first support bracket and is 20 proximate to a second edge of the microfluidic plate, the first support bracket includes a first gasket with at least one seal that is proud of the first side of the first support bracket and is positioned against the first side of the microfluidic plate, and the first indexing feature of the first support bracket and the 25 second indexing feature of the first support bracket contact the first edge and the second edge, respectively, of the microfluidic plate when the at least one seal of the first gasket is aligned with a corresponding at least one of the one or more first fluidic ports. 30
2. The apparatus of claim 1, wherein: the microfluidic plate has a second side opposite the first side, the frame has a first overlapping portion that overlaps, when viewed along a direction perpendicular to a major surface of the microfluidic plate, a first portion of the microfluidic plate that includes the second edge, the first overlapping portion is proximate to the second side of the 5 microfluidic plate, the first overlapping portion has a first clamp arm slot having a first slot width in a direction parallel to the second edge, the second side of the microfluidic plate is visible through the first clamp arm slot, 10 the apparatus is to be interfaced with a receiver of an analysis device, the receiver having a first clamp arm that is movable from an unclamped position in which the first clamp arm does not press on the second side of the microfluidic plate and does not engage with the first clamp arm slot to a clamped position in which the first clamp arm presses on the second side of the microfluidic plate and engages with 15 the first clamp arm slot, and the first slot width is larger than a width of the first clamp arm in a direction parallel to the second edge and located within the first clamp arm slot when the first clamp arm is in the clamped position. 20
3. The apparatus of claim 2 wherein: the microfluidic plate has a third edge opposite the first edge and a fourth edge opposite the second edge, the frame has a second overlapping portion that overlaps, when viewed along the direction perpendicular to the major surface of the microfluidic plate, a 25 second portion of the microfluidic plate that includes the fourth edge, the second overlapping portion is proximate to the second side of the microfluidic plate, and the second overlapping portion has a second clamp arm slot having a second slot width in a direction parallel to the fourth edge, 30 the second side of the microfluidic plate is visible through the second clamp arm slot, the receiver of the analysis device within which the apparatus is to be interfaced has a second clamp arm that is movable from an unclamped position in which the second clamp arm does not press on the second side of the microfluidic plate and does not engage with the second clamp arm slot to a clamped position in which the second clamp arm presses on the second side of the microfluidic plate and engages with the second clamp arm slot, and 5 the second slot width is larger than a width of the second clamp arm in a direction parallel to the fourth edge and located within the second clamp arm slot when the second clamp arm is in the clamped position.
4. The apparatus of claim 1, wherein: 10 there are two first fluidic ports in the microfluidic plate, and the first gasket includes two seals, each seal having a through-hole passing through the first support bracket and aligned with a different one of the first fluidic ports when the first indexing feature of the first support bracket and the second indexing feature of the first support bracket contact the first edge and the second edge, 15 respectively, of the microfluidic plate.
5. The apparatus of claim 4, wherein: the first gasket includes a support foot that is proud of the first side of the first support bracket and is positioned against the microfluidic plate, a first axis is defined between center points of the two seals of the first 20 gasket, the support foot of the first gasket is offset by a first amount from the first axis along a second axis perpendicular to the first axis and parallel to the microfluidic plate, and the support foot of the first gasket has an upper surface that contacts the 25 microfluidic plate and that is co-planar with upper surfaces of the two seals of the first gasket that are also in contact with the microfluidic plate.
6. The apparatus of claim 5, wherein the support foot of the first gasket does not serve as a seal.
7. The apparatus of claim 5, wherein the first gasket is co-molded into the first support bracket.
8. The apparatus of claim 1, wherein: the first support bracket has a second side that faces away from the first side of the first support bracket, and 5 at least two first fluidic port indexing features protrude from the second side of the first support bracket, each first fluidic port indexing feature to engage with a corresponding fluidic port indexing hole on a first fluidic port block of an analysis device to receive the apparatus. 10
9. The apparatus of claim 1, wherein: the frame includes two opposing first retaining clips with opposing surfaces that face one another, the first support bracket is positioned in between the two opposing first retaining clips, 15 the opposing surfaces of the first retaining clips are spaced apart by a first distance, and a portion of the first support bracket between the opposing surfaces of the first retaining clips has a first width in a direction spanning between the opposing surfaces of the first retaining clips that is less than the first distance.
10. The apparatus of claim 9, wherein: the first support bracket includes a third indexing feature that protrudes from the first side of the first support bracket and is proximate to a third edge of the microfluidic plate opposite the first edge of the microfluidic plate, and 25 the microfluidic plate is interposed between the first indexing feature of the first support bracket and the third indexing feature of the first support bracket.
11. The apparatus of claim 10, wherein: the microfluidic plate is rectangular and the first edge of the microfluidic 30 plate is orthogonal to the second edge of the microfluidic plate and the second edge of the microfluidic plate is orthogonal to the third edge of the microfluidic plate.
12. The apparatus of claim 10, wherein: the frame has a substantially rectangular opening, the microfluidic plate sits within the substantially rectangular opening, 5 the substantially rectangular opening has opposing side walls that face towards one another, and the first indexing feature of the first support bracket is interposed between one of the opposing side walls of the substantially rectangular opening and the first edge of the microfluidic plate and the third indexing feature of the first support 10 bracket is interposed between the other opposing side wall of the opposing side walls of the substantially rectangular opening and the third edge of the microfluidic plate.
13. The apparatus of claim 10, wherein: 15 the substantially rectangular opening has an opening width in a direction parallel to the second edge, a first indexing feature width exists between furthest-apart portions of the surfaces of the first indexing feature of the first support bracket and the third indexing feature of the first support bracket that face the opposing side walls of the 20 substantially rectangular opening, and the opening width minus the first indexing feature width is less than the first distance minus the first width.
14. The apparatus of claim 9, wherein the microfluidic plate further includes one 25 or more second fluidic ports on the first side and the apparatus further comprises: a second support bracket that is attached to the frame such that: the microfluidic plate is interposed between the second support bracket and the frame, the second support bracket floats relative to the microfluidic plate 30 and the frame, the microfluidic plate and the frame float relative to one another, and a first side of the second support bracket faces towards the microfluidic plate, wherein: the second support bracket includes a first indexing feature that protrudes from the first side of the second support bracket and is 5 proximate to the first edge of the microfluidic plate, the second support bracket includes a second indexing feature that protrudes from the first side of the second support bracket and is proximate to a fourth edge of the microfluidic plate opposite the second edge of the microfluidic plate, 10 the microfluidic plate is interposed between the second indexing feature of the first support bracket and the second indexing feature of the second support bracket, the second support bracket includes a second gasket with at least one seal that is proud of the first side of the second support 15 bracket and is positioned against the microfluidic plate, and the first indexing feature of the second support bracket and the second indexing feature of the second support bracket contact the first edge and the fourth edge, respectively, of the microfluidic plate when the at least one seal of the second gasket is aligned with a 20 corresponding at least one of the one or more second fluidic ports.
15. The apparatus of claim 14, wherein: the frame includes two opposing second retaining clips with opposing surfaces that face one another, 25 the second support bracket is positioned in between the two opposing second retaining clips, the opposing surfaces of the second retaining clips are spaced apart by a second distance, and a portion of the second support bracket between the opposing surfaces of 30 the second retaining clips has a second width in a direction spanning between the opposing surfaces of the second retaining clips that is less than the second distance.
16. The apparatus of claim 15, wherein: the second support bracket includes a third indexing feature that protrudes from the first side of the second support bracket and is proximate to the third edge of the microfluidic plate, and the microfluidic plate is interposed between the first indexing feature of the 5 second support bracket and the third indexing feature of the second support bracket.
17. The apparatus of claim 16, wherein: the frame has a substantially rectangular opening, 10 the microfluidic plate has a third edge opposite the first edge, the microfluidic plate sits within the substantially rectangular opening, the substantially rectangular opening has opposing side walls that face towards one another and that define an opening width in a direction parallel to the second edge, 15 the first indexing feature of the second support bracket is interposed between one of the opposing side walls of the substantially rectangular opening and the first edge of the microfluidic plate and the third indexing feature of the second support bracket is interposed between the other opposing side wall of the opposing side walls of the substantially rectangular opening and the third edge of the 20 microfluidic plate, the microfluidic plate has a plate width in a direction spanning between the first indexing feature of the second support bracket and the third indexing feature of the second support bracket, a second indexing feature width exists between furthest-apart portions of the 25 surfaces of the first indexing feature of the second support bracket and the third indexing feature of the second support bracket that face the opposing side walls of the substantially rectangular opening, and the opening width minus the second indexing feature width is less than the second distance minus the second width.
18. The apparatus of claim 14, wherein: there are two second fluidic ports in the microfluidic plate, and the second gasket includes two seals, each seal having a through-hole passing through the second support bracket and aligned with a different one of the second fluidic ports when the first indexing feature of the second support bracket and the second indexing feature of the second support bracket contact the first edge and the 5 fourth edge, respectively, of the microfluidic plate.
19. The apparatus of claim 18, wherein: the second gasket includes a support foot that is proud of the first side of the second support bracket and is positioned against the microfluidic plate, 10 a third axis is defined between center points of the two seals of the second gasket, the support foot of the second gasket is offset by a second amount from the third axis along a fourth axis perpendicular to the third axis and parallel to the microfluidic plate, and 15 the support foot of the second gasket has an upper surface that contacts the microfluidic plate and is co-planar with upper surfaces of the two seals of the second gasket that are also in contact with the microfluidic plate.
20. The apparatus of claim 19, wherein the support foot of the second gasket 20 does not serve as a seal.
21. The apparatus of claim 19, wherein the second gasket is co-molded into the second support bracket. 25
22. The apparatus of claim 14, wherein: the second support bracket has a second side that faces away from the first side of the second support bracket, and at least two second fluidic port indexing features protrude from the second side of the first support bracket, each first fluidic port indexing feature to engage 30 with a corresponding fluidic port indexing hole on a first fluidic port block of an analysis device to receive the apparatus.
Applications Claiming Priority (7)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US201762441927P | 2017-01-03 | 2017-01-03 | |
US62/441,927 | 2017-01-03 | ||
GB1704769.7 | 2017-03-24 | ||
GBGB1704769.7A GB201704769D0 (en) | 2017-01-03 | 2017-03-24 | Flowcell cartridge with floating seal bracket |
US15/841,109 | 2017-12-13 | ||
US15/841,109 US10357775B2 (en) | 2017-01-03 | 2017-12-13 | Flowcell cartridge with floating seal bracket |
PCT/US2017/067832 WO2018128839A1 (en) | 2017-01-03 | 2017-12-21 | Flowcell cartridge with floating seal bracket |
Publications (2)
Publication Number | Publication Date |
---|---|
NZ747845A NZ747845A (en) | 2021-02-26 |
NZ747845B2 true NZ747845B2 (en) | 2021-05-27 |
Family
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