NZ747471A - Nicotine particles and compositions - Google Patents

Abstract

The composition is formed of a plurality of particles. These particles include nicotine, a sugar, and an amino acid or short peptide.

Description

NICOTINE PARTICLES AND COMPOSITIONS This sure relates to nicotine particles and compositions that are suitable for inhalation. These nicotine particles and compositions include nicotine, a sugar, and an amino acid.

Dry powder inhalers (DPI) are known and are used to treat respiratory diseases by delivering a dry powder comprising a pharmaceutically active compound, in aerosol form through inhalation to the patients’ ain/vays. In pharmaceutical dry powders, the active pharmaceutical ingredient (API) is usually erated on the surface of larger carrier les, such as lactose for example. DPl’s operate complex isms to ensure such agglomerates disperse, break up or disaggregate before the API is d into the lungs. it may be ult to deliver nicotine particles to the lungs at inhalation at air flow rates that are within conventional smoking regime tion or air flow rates. Nicotine particles may have a tendency to agglomerate and stick to inhaler or prOcessing surfaces, especially as a size of the nicotine le deceases. Nicotine particles with an MMAD of less than about 10 micrometres tend to be increasingly thermodynamically unstable due to a high surface area to volume ratio, which provides an increasing surface free energy with this decreasing particle size, and consequently increases the tendency of particles to agglomerate and the th of the agglomerate. Forming nicotine particles may be difficult and costly.

It would be desirable to provide nicotine particles and compositions that may be formed and processed easily. It would be desirable that the nicotine particles and compositions not stick to processing surfaces or agglomerate and exhibit a stable particle size distribution. It would also be desirable that the nicotine particles and compositions be deliverable to the lungs at air flow rates that are within conventional smoking regime inhalation or air flow rates.

This disclosure is directed to a particle that comprises nicotine, a sugar and an amino acid. The particle preferably has a size in a range from about 0.5 to about 10 etres, or from about 0.5 to about 5 micrometres. The particle preferably comprises about 25 wt% or less nicotine or from about 5 to about 15 wt% ne. A free g composition may be formed by these particles.

W0 2018l002756 The particles may be formed by combining nicotine, a sugar, and an amino acid in a liquid carrier to form a liquid e. This liquid mixture is spray dried to form a plurality of particles having a size in a range from about 0.5 to about 10 micrometres or in a range from about 0.5 to about 5 etres. The plurality of particles is preferably homogenous particles.

Advantageously, the nicotine les and powder formulation described herein provide for a homogenous and stable particle size sufficient to deliver ne to the lungs of a consumer at inhalation or air flow rates that are within conventional smoking regime inhalation or air flow rates. The nicotine particles and powder formulation described herein allows these particles to be formed by spray drying to achieve a ic and lled particle size 1O distribution while minimizing agglomeration or adherence to surfaces such as processing equipment surfaces. Spray drying may provide a scalable, precise and low cost particle formation unit operation.

The term “nicotine” refers to nicotine and nicotine derivatives in any form, including but not limited to, a free-base nicotine, nicotine salt, or in a matrix such as a sugar matrix or organometallic complex.

The term “amino acid” refers to a single unmodified or modified amino acid moiety, preferably unmodified.

The term “short peptide” refers to a e comprising two or three amino acids.

The size of a le, as stated herein, preferably refers to the aerodynamic'diameter of the particle. The aerodynamic diameter of a powder system is ably measured with a cascade or. The term “MMAD” refers to the mass median aerodynamic diameter.

This disclosure relates to particles comprising nicotine, a sugar, and an amino acid.

Particles may be formed having a specific particle size distribution. In illustrative examples, about 90%, or about 95%, or about 98% of the particles have a size of about 5 micrometres or less, or about 4.5 micrometres or less, or about 4.2 micrometres or less, and about 50% of the particles have a size of about 2.5 etres or less, or about 2.1 micrometres or less. In many of these examples, about 10% of the particles have a size of about 820 nanometers or less. The particles may have a mass median aerodynamic diameter in a range from about 1 to W0 2018l002756 about 4 micrometres. Substantially all of the particles may have a particle size in a range from about 500 nanometers to about 5 micrometres.

Compositions of these particles have a specific particle size distribution. In rative examples, about 90%, or about 95%, or about 98% of the particles of the composition have a size of about 5 micrometres or less, or about 4.5 micrometres or less, or about 4.2 etres or less, and about 50% of the particles have a size of about 2.5 micrometres or less, or about 2.1 micrometres or less. In many of these examples, about 10% of the particles have a size of about 820 nanometers or less. The particles of the ition may have a mass median aerodynamic diameter in a range from about 1 to about 4 micrometres. Substantially all of the particles forming the ition may have a particle size in a range from about 500 nanometers to about 5 micrometres. The percentages ng to particle size distribution described herein are based on les by volume (% by volume).

The ne component of the particle may be a free base nicotine, a nicotine salt, or a combination thereof. The nicotine component may be a ne salt formed by combining nicotine or nicotine free base with an acid. The acid may be a stoichiometric amount of acid to the nicotine free base, or a stoichiometric excess of acid may be combined with the nicotine free base, or a stoichiometric excess of ne free base may be combined with the acid. A free base nicotine may be utilized without the addition of an acid.

The acid may be an organic acid, an inorganic acid, or a Lewis acid. Non-limiting examples of inorganic acids are hydrochloric, hydrobromic, hydroiodic, nitric, sulfuric, phosphoric, acetic, hexafluorophosphoric, and the tike. Non-limiting examples of organic acids are levulinic, , gluconic, benzoic, propionic, butyric, sulfosalicylic, maleic, lauric, malic, fumaric, succinic, tartaric, amsonic, pamoic, mesylic, aspartic, formic, acetic, nic, succinic, camphorsulfonic, fumaric, onic, lactic, mucic, para—toluenesulfonic, glycolic, glucuronic, , furoic, glutamic, benzoic, anthranilic, salicylic, phenylacetic, pyruvic, mandelic, embonic (pamoic), methanesulfonic, ethanesulfonic, pantothenic, esulfonic (besylate), stearic, sulfanilic, alginic, galacturonic, and the like. miting examples of Lewis acids are zinc chloride or zinc bromide (ZnClz / ZnBrz). These can react with nicotine to form organometallic complexes.

Useful nicotine salts include, but are not limited to, nicotine pyruvate, nicotine citrate, nicotine aspartate, nicotine lactate, nicotine bitartrate, nicotine salicylate, nicotine fumarate, W0 2018l002756 nicotine mono-pyruvate, nicotine glutamate or nicotine hydrochloride, for e. Preferred nicotine salts include, nicotine lactate, nicotine pyruvate, nicotine citrate, ne aspartate, or a ation thereof.

The pH of the particles (dissolved in water) may be in a range from about 5 to about 9.

Preferably the pH is about 7.0 or higher or in a range from 7.0 to 9.0. A pH of 9 can be reached for a particle without organic acid, while a pH of 5.0 can be obtained with the use of a strong acid or diacid when g the nicotine salt.

The particle may include an amino acid or peptide (preferably formed of three or less amino acids). The amino acid or peptide may reduce adhesion forces of the particles g 1O the composition and mitigate or t agglomeration of the particles forming the composition.

The particles forming the composition described herein thus may be a free flowing material and possess a stable relative particle size distribution during processing, ort and storage, The amino acid may be a single amino acid or molecule containing two or more amino acids such as a peptide.

Useful amino acids may include leucine, alanine, valine, isoleucine, methionine, phenylalanine, tyrosine, tryptophan, or a combination thereof. One preferred amino acid is leucine or a leucine isomer such as, L—leucine. Useful es include trileucine, for example.

The particle may include a sugar. Sugar refers to simple sugars, monosaccharides, disaccharides, and polysaccharides. Without limitation, examples of suitable sugars are lactose, e, raffinose, trehalose, fructose, dextrose, glucose, maltose, mannitol, or combinations f. Preferred sugars e trehalose or ol.

The particle may contain less than about 30 wt% nicotine. The particle may contain about 25 wt% or less nicotine, or from about 15 to about 25 wt% nicotine. The particle may contain from about 1 to about 20 wt% nicotine, or from about 10 to about 20 wt% nicotine, or from about 5 to 15 wt% nicotine. The particle may contain from about 1 to about 10 wt% nicotine or from about 5 to about 10 wt% nicotine. In some embodiments, particles that contained about 30 wt% or more nicotine agglomerated or adhered to processing surfaces when processed through a spray dryer.

W0 2018l002756 The particles forming the composition may contain less than about 30 wt% nicotine. The particles forming the composition may contain about 25 wt% or less nicotine, or from about 15 to about 25 wt% nicotine. The particles forming the composition may contain from about 1 to about 20 wt% nicotine, or from about 10 to about 20 wt% nicotine, or from about 5 to 15 wt% nicotine. The particles forming the composition may n from about 1 to about 10 wt% nicotine or from about 5 to about 10 wt% nicotine. In some embodiments, particles forming the ition that contained about 30 wt% or more nicotine ed an agglomerated or sticky composition when processed through a spray dryer.

The particle may contain about 1 to about 10 wt% amino acid. The particle may contain about 3 to about 7 wt% amino acid. The particle may contain from about 5 wt% amino acid. The addition of the amino acid, ally L—leucine for example, to the particles may reduce agglomeration or adherence to processing surfaces.

The particles forming the composition may contain about 1 to about 10 wt% amino acid.

The particles forming the composition may contain about 3 to about 7 wt% amino acid. The particles forming the composition may n from about 5 wt% amino acid. The on of the amino acid, especially L—leucine for example, to the particles forming the composition may reduce agglomeration or stickiness of the composition when processed through a spray dryer.

The particle may contain about 60 to about 95 wt% sugar. The particle may contain about 70 to about 90 wt% sugar. The particle may contain about 80 to about 85 wt% sugar.

The les forming the composition may contain about 60 to about 95 wt% sugar. The particles g the composition may contain about 70 to about 90 wt% sugar. The les forming the composition may contain about 80 to about 85 wt% sugar.

A useful particle formulation includes an amino acid being leucine, a sugar being trehalose, and a nicotine salt being nicotine lactate. The nicotine content may be from about 5 to about 15 wt% or about 9.5 wt%. The leucine content may be from about 1 to about 10 wt% The leucine t may be from about 3 to about 7 wt% or about 5 wt%. The molar ratio of acid:nicotine may about 1:1.

A useful particle formulation includes an amino acid being leucine, a sugar being trehalose, and a ne salt being nicotine citrate. The nicotine content may be from about 5 to W0 2018l002756 about 15 wt% or about 9.6 wt%. The leucine content may be from about 1 to about 10 wt% The leucine content may be from about 3 to about 7 wt% or about 5 wt%. The molar ratio of acid:nicotine may about 0.25:1.

A useful particle formulation includes an amino acid being leucine, a sugar being trehaiose, and a nicotine salt being nicotine pyruvate. The nicotine content may be from about 5 to about 15 wt%or about 9.8 wt%. The leucine content may be from about 1 to about 10 wt% The leucine content may be from about 3 to about 7 wt% or about 5 wt%. The molar ratio of acid:nicotine may about 0.621.

A useful particle formulation includes an amino acid being leucine, a sugar being 1O trehaiose, and a ne salt being nicotine aspartate. The nicotine content may be from about to about 15 wt% or about 9.3 wt%. The e content may be from about 1 to about 10 wt% The leucine t may be from about 3 to about 7 wt% or about 5 wt%. The molar ratio of acid:nicotine may about 06:1.

The les may be formed by: (1) combining a nicotine, a sugar, and an amino acid or peptide in a liquid carrier to form a liquid mixture; and (2) spray drying the liquid mixture to form les having a size in a range from about 0.5 to about 10 micrometres or in a range from about 0.5 to about 5 micrometres.

An illustrative example comprises a preparation that includes a 20% nicotine free base and an acid (e.g., lactic, pyruvic or ) ed in a liquid carrier. The molar ratio may be within the ranges 1002120 for nicotine: ic, pyruvic or lactic acid, and 0332050 for nicotinezcitric acid. The liquid mixture may be incubated at about 30°C, for example, for about 1 to about 15 minutes, to allow the ion of a stable nicotine salt solution. A pharmaceutically acceptable sugar, (for example, trehaiose or mannitol) and leucine may be added to form a liquid mixture. The liquid mixture may be spray dried by using a nozzle to atomize the liquid to form droplets, contacting the droplets with warm air, to dry and form dry particles, and collecting the particles. In this embodiment, after spray drying, 10% of the particles (by volume) may be below about 0.82 micrometre in size, 50% of the particles may be below about 2.1 etres in size and 90% of the particles may be below about 4.1 micrometres in size. The particles are substantially in the range of 0.5 to 4.2 micrometres.

The liquid carrier may be water, for example. The liquid mixture is flowable. The liquid mixture is configured to fiow through an atomization or atomizer nozzle to form the precise or W0 2018l002756 controlled particle size distribution. The particles or composition may be processed by spray drying to form a precise size distribution of particles. The particles and compositions described herein may tend to not agglomerate or stick to the surface of the spray drying equipment.

The particles and compositions described herein may be processed at a reduced ature (as compared to conventional nicotine particle formation) ing in a reduced product loss. For example, the particles and composition described herein may be spray dried at a ature in a range from about 50 to 85 degrees Celsius.

A cough suppressant may be combined with the composition. Cough suppressants include, for example, l, camphor, turpentine oil (e.g., alpha-pinene, beta-pinene) and menthol derivatives (e.g., menthyl lactate, and menthyl salicylate).

The particles and compositions described herein may then be packaged for consumption. The particles and compositions described herein may be packaged in an inhalation delivery consumable element or contained within an inhalation ry consumable element. An inhalation ry consumable element may be a capsule, for e. The capsule may be by ed in an inhalation device, such as a dry powder inhaler. The inhalation device may pierce the capsule and the fine particles may be entrained in the inhalation air for delivery to the lungs of a er.

The particles and compositions described herein and the inhalation delivery consumable element may be free of, or substantially free of carrier particles. The particles and compositions described herein and the inhalation delivery consumable element may be free of, or substantially free of particles that are greater than about 20 etres, or greater than about 50 micrometres, or greater than about 100 micrometres.

The nicotine may be ved in the liquid carrier to form the liquid mixture. The sugar may be dissolved in the liquid carrier to form the liquid mixture. The amino acid may be dissolved in the liquid carrier to form the liquid mixture. The liquid mixture may have about 20% w/v or less total solids, or about 15% w/v or less total , or a range of about 5 to 15% w/v total solids.

The nicotine particles described herein may be processed at a reduced (as compared to conventional nicotine les) temperature that may result in reduced product loss. The spray drying inlet temperature and the outlet temperature may be reduced. The spray drying W0 2018l002756 atomization pressure may be in a range from about 3 to about 7 bar, or 4 to about 6 bar, or about 5 bar.

The spray drying inlet temperature may be about 140 s Celsius or less, or about 135 degrees Celsius or less, or about 130 degrees Celsius or less, or in a range from about 100 to about 1500 degrees Celsius, or in a range from about 110 to about 140 degrees Celsius, or in a range from about 125 to about 135 degrees Celsius. The spray drying outlet temperature may be about 100 degrees s or less, or about 95 degrees Celsius or less, or about 90 degrees Celsius or less, about 85 degrees Celsius or less, or about 80 degrees s or less, or in a range from about 30 to about 90 degrees Celsius, or in a range from about 40 to about 90 degrees Celsius, or in a range from about 50 to about 85 degrees Celsius.

Specific examples are set forth in the tables below.

All scientific and cal terms used herein have meanings commonly used in the art unless otherwise specified. The definitions provided herein are to facilitate understanding of n terms used frequently herein.

As used , the singular forms “a", “an”, and “the” encompass embodiments having plural referents, unless the content clearly es otherwise.

As used herein, “or” is generally employed in its sense including “and/or” unless the content clearly es otherwise. The term “and/or" means one or all of the listed elements or a combination of any two or more of the listed elements.

As used herein, "have”, “having 71 uinclude)1 nincluding 1: fl comprise7! n , , , , comprising” or the like are used in their open ended sense, and generally mean “including, but not limited to”. It will be understood that “consisting essentially of”, “consisting of’, and the like are subsumed in “comprising,” and the like.

The words rred” and “preferably” refer to embodiments of the invention that may afford certain benefits, under certain circumstances. However, other embodiments may also be preferred, under the same or other circumstances. Furthermore, the recitation of one or more preferred embodiments does not imply that other ments are not useful, and is not intended to exclude other ments from the scope of the disclosure, including the claims.

W0 02756 is a schematic flow diagram of an illustrative method 100 of forming the particles 125. The method 100 includes combining nicotine 102, a sugar 104, and an amino acid or peptide 106 in a liquid r to form a liquid mixture 115 at block 110. Then, at block 120, the liquid mixture 115 is spray dried to form a ity of particles 125.

All the es (except Table 3 examples) are formulated by combining a nicotine free base and an acid in water (at the specified ratio) to form a stable nicotine salt solution. Then the sugar and amino acid (leucine) is combined with the nicotine salt solution to form a liquid mixture. Then the liquid mixture is atomized and dried to form dry particles that are collected to 1O from the composition.

The Table 3 examples are formulated by combining a nicotine free base with sugar and an amino acid (leucine) to form a liquid mixture. Then the liquid mixture is atomized and dried to form dry particles that are collected to from the composition.

The spray dryer was a Buchi B-290 spray dryer (available from Buchi Corp, DE, USA).

The liquid mixture was provided to the spray dryer at a flow rate of 2 ml/min at 5 bar atomization pressure. The outlet temperature was about 80 degrees Celsius for examples utilizing trehalose. Table 1 below describes lactic acid formulations. Table 2 below describes pyruvic acid formulations. Table 3 below describes no acid ations. Table 4 and Table 5 report the particle size distribution of s examples.

Table 1 - Lactic Acid Nicotine Powder Formulations Example ation pH of Comments powder solution L1 10% Nicotine, Lactic acid (1:1), 7.3 Small amount of powder adhering 85% Trehalose to spray dryer surface L2 15% Nicotine, Lactic acid (1:1), 7.0 Small amount of powder adhering 77% Trehalose to spray dryer surface _ 10 _ L3 10% Nicotine, Lactic acid (1 :1), 7.5 Free flowing powder — no 80% Trehaiose, 5% e adherence L4 15% Nicotine, Lactic acid (1 :1), Free flowing powder — no 72% Trehaiose, 5% Leucine adherence % Nicotine, Lactic acid (1:1), Free flowing powder — no 64% Trehaiose, 5% Leucine adherence Table 2 - Pyruvic Acid Nicotine Powder Formulations Example Formulation pH of Comments powder solution P1 10% Nicotine, Pyruvic acid (0.6:1), 7.5 Powder adhering to spray dryer 87% Trehaiose surface, cohesive powder P2 15% Nicotine, Pyruvic acid , 7.8 Cohesive powder, some static 80% Trehaiose charge F3 10% Nicotine, Pyruvic acid ), 7.7 Free flowing powder —— no 82% Trehaiose, 5% Leucine adherence, some static charge P4 15% Nicotine, Pyruvic acid , 7.8 Free flowing powder — no 75% Trehaiose, 5% Leucine adherence P5 20% Nicotine, Pyruvic acid , 7.7 Free flowing powder — no 68% Trehaiose, 5% Leucine adherence Table 3 - No Acid (Free Base) Nicotine Powder Formulations Example Formulation pH of Comments powder solution N1 10% Nicotine, 90% Trehalose 9.3 Some powder adhering to spray dryer surface N2 15% Nicotine, 85% Trehalose 9.5 Some powder adhering to spray dryer e N3 10% Nicotine, 85% Trehalose, 5% 8.6 Free flowing powder — no Leucine adherence, some static charge N4 15% Nicotine, 80% Trehalose, 5% 8.7 Free g powder — no Leucine adherence N5 20% Nicotine, 75% Trehalose, 5% 8.8 Free g powder — no Leucine adherence Table 4 — Particle Size Distribution — reported in micrometres Example X90 VMD L1 1.43 3.54 1.81 1 1.62 J1 3.75 1.97 1 1.89 3.86 2.14 1 2.14 3.99 2.35 1 1.95 3.90 2.19 1 1.54 3.47 1.85 1 1.53 3.54 1.86 P3 1 0.66 1 1.48 3.54 1.84 P4 1 0.72 1 1.78 i 3.79 2.06 _ 12 _ 1.43 3.54 1.81 l 1.62 3.75 1.97 l 1.89 3.86 2.14 l 2.14 3.99 2.35 1.95 3.90 2.19 1.54 l 3.47 1.85 X10 refers to size of particle where 10% of particles, by , are less than this size.

X50 refers to size of particle where 50% of particles, by volume, are less than this size.

X90 refers to size of particle where 90% of particles, by volume, are less than this size.

VMD refers to volume mean diameter.

Table 5 — Further Formulations Example Formulation X10 X50 X90 VMD MMAD 1 10% Nicotine, Lactic Acid (1:1), 092 2.17 4.15 2.4 3.8 80% Trehalose, 5% Leucine 2 10% Nicotine, c Acid (120.6), 1.04 2.58 5.08 2.9 4.0 82% Trehalose, 5% Leucine 3 10% Nicotine, Citric Acid (1:0.25), 0.81 2.34 5.48 2.8 3.5 82% Trehalose, 5% Leucine 4 10% Nicotine, Aspartic Acid (1:06), 0.82 2.24 4.96 2.6 4.2 80% Trehalose, 5% Leucine 5% ne, Lactic Acid (1 :1), 0.7 1.5 3,0 1.5 2.5 82% Trehalose, 10% Leucine W0 2018l002756 _ 13 _

Claims (13)

CLAIMS 1.:

1. A particle, comprising: nicotine; a sugar; and an amino acid or short peptide.

2. The particle of claim 1 wherein, the particle has a size in a range from about 0.5 to about 10 micrometres or in a range from about 0.5 to about 5 micrometres

3. The particle of claim 1 or 2 wherein, the amino acid comprises leucine, e, valine, isoleucine, methionine, phenylalanine, tyrosine, or tryptophan.

4. The particle of any one of the preceding claims, wherein the sugar comprises trehalose, 15 or mannitol.

5. The particle of any one of the preceding claims, wherein the nicotine comprises a ne salt selected from the group ting of nicotine lactate, nicotine pyruvate, nicotine citrate, and nicotine aspartate.

6. ' The particle of any one of the preceding , wherein the amino acid comprises leucine and the sugar ses trehalose and the nicotine ses nicotine lactate.

7. The particle of any one of claims 1 to 5, wherein the nicotine comprises nicotine citrate 25 or nicotine aspartate. W0 2018l002756

8. The particle of any one of the preceding claims, n the particle comprises about 25 wt% or less nicotine or from about 5 to about 15 wt% nicotine.

9. The particle of any one of the preceding claims, n the particle comprises about 60 wt% to about 95 wt% sugar or from about 70 to about 90 wt% sugar.

10. The particle of any one of the preceding claims, wherein the particle comprises about 1 wt% to about 10 wt% amino acid or from about 3 to about 7 wt% amino acid.

11. A composition comprising a plurality of particles according to any one of the preceding claims, n about 90% of the plurality of particles have a particle size of about 4.5 micrometres or less, and about 50% of the plurality of particles have a particle size of less than about 2.5 micrometres.

12. A method, comprising: combining nicotine, a sugar, and an amino acid or short e in a liquid carrier to form a liquid mixture; and spray drying the liquid mixture to form a plurality of particles having a size in a range 20 from about 0.5 to about 10 micrometres or in a range from about 0.5 to about 5 micrometres.

13. The method according to claim 12, wherein the ing step ses combining a cough suppressant with the nicotine, a sugar, and an amino acid or peptide in the liquid carrier 25 to form the liquid mixture.