NZ743660A - Crystalline forms of hydrochloride salts of thienopyrimidine compound - Google Patents

Abstract

The present invention relates to a crystalline form of a hydrochloride salt of N-(3-(2-(4-(4-methylpiperazin-1-yl)phenylamino)thieno[3,2-d]pyrimidin-4-yloxy)phenyl)acrylamide, and a pharmaceutical composition containing the same. The crystalline form of the hydrochloride salt of the compound can be easily used for preparing a pharmaceutical composition containing the same as an active ingredient. easily used for preparing a pharmaceutical composition containing the same as an active ingredient.

Description

Description Title of Invention: CRYSTALLINE FORMS OF HY- DROCHLORIDE SALTS OF PYRIMIDINE COMPOUND Technical Field The present invention relates to hydrochloride salts of a thienopyrimidine compound, and pharmaceutical compositions ning the same. More specifically, the present invention s to hydrochloride salts of N — (3—(2—(4—(4—methylpiperazin—1—yl)phenylamino)thieno[3,2—d]pyrimidin—4—yloxy)phenyl )acrylamide, and pharmaceutical compositions ning the same.

Background Art The compound of Formula 1 below, whose compound name is N — (3—(2—(4—(4—methylpiperazin—1—yl)phenylamino)thieno[3,2—d]pyrimidin—4—yloxy)phenyl )acrylamide is disclosed in PCT application . The compound has a selective tory activity for a mutant epidermal growth factor receptor tyrosine kinase.

[Formula 1] \ QM m0 /osfl N WI / Additionally, the above reference discloses the method for preparing the compound of Formula 1.

However, the compound of Formula 1 ed in the above cited reference was prepared as an amorphous solid, which is a form generally less suitable for large—scale production of ceutical drugs. In on, the rather poor solubility of the compound of Formula 1 obtainable by the method in the above cited reference left room for improvement.

Accordingly, there is a need for suitable solid forms, preferably crystalline forms, of the compound of Formula 1 that can fully comply with the strict requirements and details thereof regarding pharmaceutical solid forms and formulations while having improved water solubility.

One sort of solid forms are salt forms of active ingredients, 6. g. acid addition salts of basic active ingredients able by reaction with acids. It is a challenging endeavor WO 16192 2016/015535 to identify suitable salt forms with appropriate solid state properties as there are many salt formers and potentially several polymorphs for each salt form. The present ors have discovered that hydrochloride salts of the nd of Formula 1, es- pecially the lline forms thereof, have excellent overall physicochemical charac— teristics required pharmaceutically including, for example, enabling long—term stable maintenance without requiring particular storage ions, etc, while having excellent water—solubility, thereby completing the present invention.

Disclosure of Invention Technical Problem An object of the present invention is to provide a hydrochloride salt of the thienopy— rimidine compound of Formula 1, and a pharmaceutical composition containing the S 81116.

Solution to Problem To achieve the above object, in one aspect of the present invention, there is provided a hydrochloride salt, in particular a crystalline form of a hydrochloride salt, of the compound of Formula 1 shown below.

[Formula 1] N 0QL K/NQ N WI / In a further aspect the crystalline form of the hydrochloride salt of the compound of Formula 1 is a monohydrochloride.

In a further aspect the crystalline form of the hydrochloride salt of the compound of Formula 1 is a ochloride.

In a further aspect the crystalline form of the hydrochloride salt of the compound of Formula 1 is a hydrate.

In a further aspect the crystalline form of the hloride salt of the compound of Formula 1 is a monohydrate.

In a further aspect the crystalline form of the hydrochloride salt of the compound of Formula 1 is a trihydrate.

In a further aspect the crystalline form of the hydrochloride salt of the compound of Formula 1 is a dihydrate.

Specific es of the above crystalline forms are as shown below: A crystalline form of a ochloride e, preferably a monohydrate (2HC1- 1H ), of the compound of Formula 1 having an X-ray powder diffraction (XRPD) pattern sing peaks at diffraction angles of 26 = 5.6% 0.20, 21.1°i 0.20 and 27.30: 0.20 when irradiated with a Cu-Kor light source. This crystalline form may further comprise diffraction peaks at 26 = 11.l°i 0.20, 14.0% 0.20 and 20.8% 02" when irradiated with a Cu-Ka light source; A crystalline form of a dihydrochloride hydrate, preferably a monohydrate (2HCl- 1H ), of the compound of Formula 1 having an X-ray powder diffraction (XRPD) pattern comprising peaks at diffraction angles of 26 = 6.4°i 0.20, 12.8% 0.20, 20.8% 0.20 and 22.0°i 02" when irradiated with a Cu-Ka light source. This crystalline form may further comprise diffraction peaks at 26 = 8.1% 0.20, 97% 02°, 16.0% 0.20, 24.l°i 0.20, 26.3% 02°, and 271°: 0.20 when irradiated with a Cu-Ka light source; A crystalline form of a dihydrochloride hydrate, preferably a trihydrate (2HCl-3H20), of the compound of Formula 1 having an X—ray powder ction (XRPD) pattern comprising peaks at diffraction angles of 26 = 4.6% 0.20, 8.6% 0.20 and 15.8% 0.20 when ated with a Cu—Ka light source. This crystalline form may further comprise diffraction peaks at 26 = 17.2% 0.20, 19.7% 0.20, 25.1% 0.20, and 26.3% 0.20 when irradiated with a Cu—Kor light source; A crystalline form of a dihydrochloride hydrate, preferably a trihydrate (2HCl-3HZO), of the compound of Formula 1 having an X—ray powder diffraction (XRPD) pattern comprising peaks at diffraction angles of 26 = 6.4°i 0.20, 7.0°i O.2°, 12.8°w_L 0.20 and 21.0°i 02" when irradiated with a Cu-KOt light source. This crystalline form may further comprise ction peaks at 26 = 15.5% 02°, 18.201L 0.20 and 27.9% 0.20 when irradiated with a Cu—ch light source; A crystalline form of a monohydrochloride e, preferably a monohydrate (lHCl- 1H20), of the compound of Formula 1 having an X—ray powder diffraction (XRPD) pattern comprising peaks at diffraction angles of 26 = 7.801 0.20, 22.5Oi 0.20 and 25.7Oi 0.20 when irradiated with a Cu-KOL light . This lline form may further comprise diffraction peaks at 26 = 10.7°i 0.2°, 13.001L 0.20, 18.6% 0.20, 19.1% 02°, 22.0°i 0.20, 24.6% 0.20 and 25.3°i 0.20 when irradiated with a Cu-Ka light source; A crystalline form of a monohydrochloride hydrate, preferably a ate (lHCl-2H ), of the compound of Formula 1 having an X—ray powder diffraction (XRPD) pattern comprising peaks at diffraction angles of 26 = 7.5Oi 0.20, 15 . lot 02" and .004; 0.20 when irradiated with a Cu-KOL light . This lline form may r comprise diffraction peaks at 26 = 21.2°i 0.2° and 25.1°i 0.20 when irradiated with a Cu—Ka light source; A crystalline form of a monohydrochloride e, preferably a dihydrate (lHCl-2H ), of the compound of Formula 1 having an X-ray powder diffraction (XRPD) pattern comprising peaks at diffraction angles of 26 = 8.70: 0.20, 194°: 0.20 and 23. 1% 0.20 when irradiated with a Cu—Ka light source. This crystalline form may further comprise diffraction peaks at 26 = 11.60: 0.20, 17.5% 02° and 26.10: 0.20 when irradiated with a Cu—Ka light source; In a further aspect each crystalline form of the hydrochloride salt as described herein is in substantially pure form.

The term "substantially pure" as used herein means at least 95% pure, preferably 99% pure, where 95% pure means not more than 5%, and 99% pure means not more than 1%, of any other form of the compound of Formula 1 being present (other crystalline form, amorphous form, etc).

In a further aspect of the present ion, there is provided a pharmaceutical com— position containing a hydrochloride salt of the compound of Formula 1 or one of the crystalline forms of the hloride salt as described herein and at least one pharma— ceutically acceptable carrier and/or t.

The pharmaceutical composition can be used for the treatment of cancer induced by epidermal growth factor receptor tyrosine kinase or a mutant thereof.

Advantageous Effects of Invention The hloride salt of the compound of Formula 1, in particular the crystalline forms according to the present invention has excellent overall physicochemical charac— teristics, i.e., water solubility, hygroscopicity. chemical stability, etc, and thus they can be easily used for the preparation of a pharmaceutical composition ning the same as an active ingredient.

Brief Description of gs FIGS. 1A to 1G show X—ray powder diffraction (XRPD) patterns of crystalline forms of the salts of the compound of Formula 1 according to Examples of the present invention.

FIG. lH shows an X—ray powder diffraction (XRPD) pattern of an amorphous form of the salts of the nd of Formula 1 according to Comparative e of the present invention.

FIGS. 2A to 2F show graphs of differential scanning calorimetry (DSC) of crystalline forms of the salts of the compound of Formula 1 according to Examples of the present ion. shows a graph of differential scanning calorimetry (DSC) of an amorphous form of the salts of the compound of Formula 1 according to Comparative Example of the present invention.

FIGS. 3A to 3F show graphs of c vapor sorption (DVS) of crystalline forms of the salts of the compound of a 1 according to Examples of the present invention. shows a graph of dynamic vapor sorption (DVS) of an amorphous form of the salts of the nd of Formula 1 according to Comparative Example of the present invention.

FIGS. 4A to 4G show graphs of 13C cross polarization/magic angle spinning total suppression of sidebands solid state nuclear magnetic resonace S TOSS ssNMR) of crystalline forms of the salts of the compound of Formula 1 according to Examples of the present invention. shows a graph of 13C CP/MAS TOSS ssNMR of an ous form of the salts of the compound of Formula 1 according to a Comparative Example of the present invention.

Mode for the Invention Unless otherwise d, all terms including technical and scientific terms used herein have the same meaning as commonly understood within the context by one of ordinary skill in the art to which this invention belongs. However, unless otherwise specified, the term described below will have the meaning indicated below over the entire specification: As used herein, the term "about" refers to being within 5% of a particular value or range, and preferably within 1% to 2%. For example, "about 10%" refers to 9.5% to .5%, and preferably, 9.8% to 10.2%. For another example, "about 100°C" refers to 95°C to 105°C, and preferably, 98°C to 102°C.

Unless otherwise specified, it must be apparent to a skilled practitioner that the values of peaks from X—ray powder diffraction s ed in this invention are as— sociated with experimental errors typically able in this field. Specifically, a peak is interpreted as to be located within 105° of the value reported herein. More specifically, a peak is interpreted as to be located within i—O.2° of the value reported herein.

Unless ise specified, it must be apparent to a skilled practitioner that the values of peaks from solid state nuclear magnetic resonance (ssNMR) s reported in this invention are associated with experimental errors typically observable in this field. Specifically, a chemical shift is interpreted as to be located within i0.5 ppm of the value reported herein. More ically, a chemical shift is interpreted as to be located within i0.2 ppm of the value reported herein.

Hydrochloride salts of the compound of Formula 1 The t invention provides a hydrochloride salt of the compound of Formula 1 below, i.e., N - (3—(2—(4—(4—methylpiperazin—1—yl)phenylamino)thieno[3,2—d]pyrimidin—4—yloxy)phenyl )acrylamide.

[Formula 1] The compound of Formula 1 above (free base) may be prepared according to the con— nal procedure described in , which is hereby incorporated by reference in its entirety.

The compound of Formula 1 disclosed in the above reference is in an amorphous form, and is a poorly soluble compound having water solubility below 0.1 ug/mL.

Generally, it is known that the conversion of a free base into a salt form can help solubilize a water—insoluble pharmaceutical material. However, the salt should also possess the overall ochemical properties which are required pharmaceutically, such as reproducibility for the preparation of particular crystalline polymorphs, a high degree of crystallization, stability of crystalline forms, chemical stability, non— hygroscopicity, etc.

For the selection of an appropriate salt type for the compound of Formula 1, salts of the nd of Formula 1 were prepared using various acids and solvents according to various conditions and procedures, and the physicochemical properties of the thus— obtained salts were evaluated. Among the thus—obtained high number of salts and types of lline forms the hydrochloride salts of the nd of Formula 1, in particular the various crystalline forms described herein showed the best overall physicochemical properties which are required pharmaceutically, such as repro— ducibility for the preparation of particular crystalline polymorphs, a high degree of crystallization, stability of crystalline forms, al stability, non-hygroscopicity, etc In one embodiment of the present invention, provided are crystalline hydrochloride salts of the nd of Formula 1. In a particular embodiment of the present invention, these lline hydrochloride salts are hydrates. In another specific em— bodiment, the crystalline hydrochloride salt is dihydrochloride. In a further specific embodiment, this dihydrochloride salt is a hydrate. In yet another ic embodiment, the crystalline hydrochloride salt is monohydrochloride. In a still further specific em— bodiment, this drochloride salt is a hydrate.

Crystalline form of salts of the compound of Formula 1 The salts of the compound of Formula 1 may be prepared in a crystalline form, an amorphous form, or a mixture thereof, and preferably in a crystalline form. The crystalline form of a hydrochloride salt of the Formula 1 compound has excellent stability and is thus preferable in that it has a physicochemical property which fa— cilitates its formulation.

According to the present invention, the compound of Formula 1 may be prepared in various crystalline forms of a hydrochloride, e. g., a lline form (Type A) of a di— hydrochloride hydrate, preferably monohydrate (2HCl- leO); a crystalline form (Type B) of a dihydrochloride e, ably drate (2HCl-1H20); a crystalline form (Type A) of a dihydrochloride hydrate, preferably trihydrate (2HCl-3H20); a crystalline form (Type B) of a dihydrochloride hydrate, preferably trihydrate (2HCl-3H ); a crystalline form of a drochloride hydrate, preferably monohydrate (lHCl-1H20); a crystalline form (Type A) of a drochloride hydrate, preferably dihydrate (lHCl~2HZO); and a crystalline form (Type B) of a monohydrochloride e, preferably dihydrate (lHCl-ZHZO).

Among the crystalline forms of hydrochloride salts, as examined in Test Example 1 described later, the crystalline form (Type A) of the ochloride hydrate, preferably monohydrate (2HCl~lH20), showed the highest water solubility, and it may be advantageous from the s of non—hygroscopicity/non-dehumidification and stability, and thus may be preferable as an active ingredient for a pharmaceutical com— Each of the crystalline forms according to the present invention will be explained more ically herein below.

In an exemplary embodiment (ex. 1), the present invention provides a crystalline form (Type A) of a ochloride hydrate, preferably monohydrate (2HCl-lH20), of the compound of Formula 1.

This crystalline form (ex. 1) exhibits an XRPD pattern comprising peaks at diffraction angles of 26 = .2° and 27.3Oi0.2° when irradiated with a Cu—Kor light source (XRPD l— l ).

More specifically, the above crystalline form (ex. 1) exhibits an XRPD pattern comprising peaks at diffraction angles of 26 = 5.6Oi0.2°, 21.1"i0.2O and 27.3Oi0.2° when irradiated with a Cu—Ka light source —Z).

More specifically, the above crystalline form (ex. 1) has an XRPD pattern comprising peaks at diffraction angles of 29 = 5.601020, ll.1°4_-0.2° and 27.3Oi0.2° when irradiated with a Cu—Ka light source (XRPDl—3).

More specifically, the above crystalline form (ex. 1) has an XRPD pattern comprising peaks at diffraction angles of 26 = 5.6Oi0.2°, ll.l°i0.2°, 21.l°i0.2°, and 27.3Oi0.2° when irradiated with a Cu-Ka light source (XRPD1-4).

More specifically, the above crystalline form (ex. 1) has an XRPD pattern comprising peaks at diffraction angles of 20 = .2°, 11.1°i0.2°, 14.0°i0.2° and 27.3°i0.2° when irradiated with a Cu-Ka light source (XRPD1-5).

More ically, the above crystalline form (ex. 1) has an XRPD pattern comprising peaks at diffraction angles of 20 = 5.6°iO.2°, 11.1°iO.2°, 14.0°iO.2°, 0.2°, and 27.3°i0.2° when irradiated with a Cu—Ka light source (XRPD1—6).

More ically, the above crystalline form (ex. 1) has an XRPD pattern comprising peaks at diffraction angles of 20 = 5.6°i0.2°, 11.1°i0.2°, 14.0°i0.2°, 20.8°i0.2°, 21.1°i0.2°, and 27.3°i0.2° when irradiated with a Cu-ch light source (XRPD1—7).

More specifically, the above crystalline form (ex. 1) has an XRPD pattern comprising peaks at diffraction angles of 26 = 5.6°i0.2°, 10.7°i0.2°, 11.l°i0.2°, 14.0°i0.2°, .8°i0.2°, 21.1°i0.2°, 22.5°i0.2°, and 27.3°i0.2° when irradiated with a Cu-Kd light source (XRPDl—S).

These peaks may be those having a relative intensity (1/10) of about 10% or more.

The above crystalline form (ex. 1) may have a water content of about 3.1% (theoretical water content value of 3.11%) and a melting point of about 202°C to 225°C.

The above crystalline form (ex. 1) may have a broad endothermic peak in the range of -150°Cand an endothermic peak(s) at about 221°C by a DSC with a heating rate of °C /min.

The above crystalline form (ex. 1) may have an ermic peak which has a starting point at about 49°C and its lowest point at about 110°C, endothermic peaks at about 221°C and about 253°C, and an exothermic peak at about 265°C in a DSC (10°C/min).

The above crystalline form (ex. 1) may show reversible water sorption and tion about 3% in the complete range of 0—90% RH, with a very low level of change in the region with a relative humidity of 10% to 90% in a DVS.

The above crystalline form (ex. 1) may have a 13C CP/MAS TOSS ssNMR spectrum comprising peaks at the following 13C chemical shifts: 44.6 i 0.2 ppm and 56.6 i 0.2 ppm (ssNMRl— 1).

More specifically, the above crystalline form (ex. 1) may have a 13C CP/MAS TOSS ssNMR spectrum sing peaks at the following l3C al shifts: 44.6 i 0.2 ppm, 45.4 i 0.2 ppm, 50.8 i 0.2 ppm and 56.6 i 0.2 ppm (ssNMR1—2).

The above crystalline form (ex. 1) may have a 13C CP/MAS TOSS ssNMR spectrum comprising peaks at the following 13C chemical : 149.6 i 0.2 ppm, 152.6 i 0.2 ppm and 164.3 4.- 0.2 ppm (ssNMR1—3).

More specifically, the above crystalline form (ex. 1) may have a 13C CP/MAS TOSS ssNMR um comprising peaks at the following l3C chemical shifts: 116.5 J; 0.2 ppm, 130.7 i 0.2 ppm, 146.8 4.- 0.2 ppm, 149.6 1 0.2 ppm, 1526 i- 0.2 ppm and 1643 i 0.2 ppm (ssNMR1—4).

More specifically, the above crystalline form (ex. 1) may have a 13C CP/MAS TOSS ssNMR spectrum comprising peaks at the following l3C chemical shifts: 44.6 i 0.2 ppm, 56.6 i 0.2 ppm,149.6 i 0.2 ppm, 152.6 i 0.2 ppm and 164.3 i 0.2 ppm (ssNMRl—S).

The above crystalline form (ex. 1) may have (a) an X—ray powder diffraction (XRPD) pattern sing peaks at diffraction angle 20 values of 5.6% 02" and 27.3% 0.20 when ated with a Cu—Ka light source; and (b) a 13C CP/MAS TOSS ssNMR spectrum comprising peaks at the following l3C chemical shifts: 44.6 i 0.2 ppm and 56.6 i 0.2 ppm.

The above crystalline form (ex. 1) may have (a) an X—ray powder diffraction (XRPD) pattern comprising peaks at diffraction angle 26 values of 5.6% 0.2° and 27.3% 02" when irradiated with a Cu—Koz light source; and (b) a 13C CP/MAS TOSS ssNMR spectrum comprising peaks at the following l3C chemical shifts: 149.6 i 0.2 ppm, 152.6 i 0.2 ppm and 164.3 i 0.2 ppm.

The above crystalline form (ex. 1) may also be characterized by any other com— bination of lists of XRPD peaks (XRPDl—l to XRPD1—7) and 13C chemical shifts (ssNMRl-l to ssNMRl-S) as listed above.

In another exemplary embodiment (ex.2), the present invention also provides a crystalline form (Type B) of a dihydrochloride hydrate, preferably drate (2HCl-1H20), of the compound of Formula 1.

This crystalline form (ex.2) exhibits an XRPD pattern comprising peaks at diffraction angles of 20 = 6.4%0.2°, 12.8%0.2°, 20.8%0.2O and 22.0%0.2O when irradiated with a Cu-KOt light source (XRPD2—1).

More specifically, the above crystalline form (ex.2) has an XRPD pattern sing peaks at diffraction angles of 26 = 6.4% 0.20, 8.1% 0.20, 9.7% 0.20, 12.8% 0.20, 16.0% 02°, 20.8% 0.20, 22.0% 0.20, 24.1% 0.20, 26.3% 02°, and 27.1% 0.20 when irradiated with a Cu-KOL light source (XRPD2—2).

More specifically, the above crystalline form (ex.2) has an XRPD pattern sing peaks at diffraction angles of 20 = 6.4% 0.20, 8.1% 0.20, 9.7% 0.20, 12.8% 0.20, 16.0% 0.20, 20.8% 0.20, 22.0% 0.20, 24.1% 0.20, 26.3% 0.20, 26.8% 0.20, 27.1% 02°, and 28.1% 0.20 when ated with a Cu-KOt light source (XRPD2—3).

These peaks may be those having a relative intensity of about 20% or more.

The above crystalline form (ex.2) may have a 13C CP/MAS TOSS ssNMR um sing peaks at the following 13C al shifts: 43.4 i 0.2 ppm and 45 .2 i- 0.2 ppm (ssNMR2-1).

More specifically, the above crystalline form (ex.2) may have a 13C CP/MAS TOSS ssNMR spectrum comprising peaks at the following 13C al shifts: 43.4 i 0.2 ppm, 45.2 i 0.2 ppm, 49.8 i 0.2 ppm, 51.3 i 0.2 ppm and 53.3 i 0.2 ppm (ssNMR2-2).

The above crystalline form (ex.2) may have a 13C CP/MAS TOSS ssNMR spectrum comprising peaks at the following 13C al : 117.0 i 0.2 ppm, 149.8 1 0.2 ppm and 165.2 i 0.2 ppm (ssNMR2—3).

More specifically, the above lline form (ex.2) may have a 13C CP/MAS TOSS ssNMR um comprising peaks at the following l3C chemical shifts: 117.0 i 0.2 ppm, 120.4 i 0.2 ppm, 128.7 i 0.2 ppm, 149.8 i 0.2 ppm, 151.7 i 0.2 ppm and 165.2 i 0.2 ppm (ssNMR2—4).

More specifically, the above crystalline form (ex.2) may have a 13C CP/MAS TOSS ssNMR spectrum sing peaks at the following l3C chemical : 43.4 i 0.2 ppm, 45.2 i 0.2 ppm, 117.0 i 0.2 ppm, 149.8 i 0.2 ppm and 165.2 i 0.2 ppm (ssNMR2—5).

The above crystalline form (ex.2) may have (a) an X—ray powder diffraction (XRPD) pattern comprising peaks at diffraction angle 26 values of 6.4Oi0.2°, 12.8°i0.2°, 20.8"i0.2O and 0.2° when irradiated with a Cu—Ka light source; and (b) a 13C CP/MAS TOSS ssNMR spectrum comprising peaks at the following 13C chemical shifts: 43.4 i 0.2 ppm and 45.2 i 0.2 ppm.

The above crystalline form (ex.2) may have (a) an X—ray powder diffraction (XRPD) pattern comprising peaks at diffraction angle 26 values of 6.40102", l2.8°i0.2°, 20.8"i0.2O and 0.2° when irradiated with a Cu—ch light source; and (b) a 13C CP/MAS TOSS ssNMR spectrum comprising peaks at the following 13C chemical shifts: 117.0 i 0.2 ppm, 149.8 i 0.2 ppm and 165.2 i 0.2 ppm.

The above crystalline form (ex.2) may also be characterized by any other com— bination of lists of XRPD peaks (XRPD2—1 to XRPD2—3) and 13C chemical shifts (ssNMR2—1 to ssNMR2—5) as listed above.

In another exemplary embodiment (ex.3), the present invention also provides a crystalline form (Type A) of a dihydrochloride hydrate, preferably trihydrate (2HCl-3H ), of the compound of a 1.

This crystalline form (ex.3) exhibits an XRPD pattern comprising peaks at diffraction angles of 26 = 4.6°iO.2°, 8.60~|_-0.2O and 15.8"w_L0.2O when irradiated with a Cu—Ka light source (XRPD3—1).

More specifically, the above crystalline form (ex.3) has an XRPD pattern comprising peaks at diffraction angles of 20 = 4.6°iO.2°, 8.6°4_-0.2°, 15.8°iO.2°, 17.2°i0.2°, 19.7°i0.2°, 25.1°i0.2°, and 26.3°i0.2° when irradiated with a Cu—Ka light source (XRPD3-2).

More specifically, the above crystalline form (ex.3) has an XRPD pattern comprising peaks at diffraction angles of 20 = .2°, 8.6°iO.2°, 15.8°iO.2°, 17.2°i0.2°, 19.7°i0.2°, 20.1°i0.2°, 21.1°i0.2°, 23.5°i0.2°, 25.1°i0.2°, and 26.3°i0.2° when irradiated with a Cu-Ka light source (XRPD3—3).

These peaks may be those having a relative intensity of about 15% or more.

The above crystalline form (ex.3) may have endothermic peaks at about 51°C and about 95°C (10°C /min) and endothermic peaks at about 178°C and about 218°C in a DSC (10°C/min).

The above crystalline form (ex.3) may have a water content of about 10.1% etical water content value of 8.8%) and a melting point of about 205°C to 210°C.

The above crystalline form (ex.3) may have a hygroscopicity ed at a very low level in the region with a relative humidity of 10% to 40% in a DVS, but the hygro— scopicity in the region with a relative humidity of 40% or higher may be ed to be about 9%.

The above crystalline form (ex.3) may have a 13C CP/MAS TOSS ssNMR spectrum comprising peaks at the following 13C chemical shifts: 45.0 i 0.2 ppm and 53.8 i 0.2 ppm (ssNMR3— 1).

More specifically, the above lline form (ex.3) may have a 13C CP/MAS TOSS ssNMR spectrum comprising peaks at the following l3C chemical shifts: 116.4 i 0.2 ppm, 117.6 i 0.2 ppm, 131.4 i 0.2 ppm, 149.3 i 0.2 ppm, and 1502 i 0.2 ppm (ssNMR3—2).

The above crystalline form (ex.3) may have a 13C CP/MAS TOSS ssNMR spectrum comprising peaks at the following 13C chemical : 117.6 1 0.2 ppm and 150.2 1 0.2 ppm (ssNMR3—3).

More specifically, the above lline form (ex.3) may have a 13C CP/MAS TOSS ssNMR spectrum comprising peaks at the ing l3C chemical shifts: 116.4 i 0.2 ppm, 117.6 i 0.2 ppm, 131.4 i 0.2 ppm, 149.3 i 0.2 ppm, and 150.2 i 0.2 ppm (ssNMR3—4).

More specifically, the above crystalline form (ex.3) may have a 13C CP/MAS TOSS ssNMR um comprising peaks at the following l3C chemical shifts: 45.0 i 0.2 ppm, 53.8 i 0.2 ppm, 117.6 4.- 0.2 ppm and 150.2 4.- 0.2 ppm (ssNMR3—5).

The above crystalline form (ex.3) may have (a) an X—ray powder diffraction (XRPD) pattern comprising peaks at diffraction angle 20 values of 4.6°i 0.2°, 8.6°i 0.2°, and 15.8°i 0.2° when irradiated with a Cu— Koz light source; and (b) a 13C solid state NMR spectrum comprising peaks at the following 13C chemical shifts: 45.0 i 0.2 ppm and 53.8 i 0.2 ppm.

The above crystalline form (ex.3) may have (a) an X—ray powder diffraction (XRPD) pattern comprising peaks at diffraction angle 26 values of 4.6°i 02°, 86°: 02°, and 158°: 0.2° when irradiated with a Cu- Ka light source; and (b) a 13C solid state NMR spectrum comprising peaks at the following l3C chemical shifts: 117.6 i 0.2 ppm and 150.2 i 0.2 ppm.

The above crystalline form (ex.3) may also be characterized by any other com— on of lists of XRPD peaks (XRPD3—1 to XRPD3—3) and 13C chemical shifts (ssNMR3-l to ssNMR3-5) as listed above.

In another exemplary embodiment (ex.4), the present invention also es a crystalline form (Type B) of a dihydrochloride e, preferably trihydrate (2HCl-3H ), of the compound of Formula 1.

This crystalline form (ex.4) exhibits an XRPD pattern comprising peaks at diffraction angles of 26 = 6.4°i0.2°, .2°, 12.8°i0.2° and 21.0°i0.2° when ated with a Cu-ch light source (XRPD4—1).

More specifically, the above crystalline form (ex.4) has an XRPD pattern comprising peaks at diffraction angles of 26 = 6.4°i0.2°, 7.0°i0.2°, 12.8°i0.2°, 15.5°i0.2°, 18.2°i0.2°, 21.0°i0.2°, and 27.9°i0.2° when irradiated with a Cu—Kor light source (XRPD4—2).

More specifically, the above crystalline form (ex.4) has an XRPD n comprising peaks at ction angles of 26 = 6.4°i0.2°, 7.0°i0.2°, 0.2°, 13.2°i0.2°, 14.1°i0.2°, 15.5°i0.2°, 18.2°i0.2°, 19.4°i0.2°, 20.5°i0.2°, 21.0°i0.2°, 23.0°i0.2°, 24.5 °i0.2°, 25 .8°i0.2°, and 27.9°i0.2° when irradiated with a Cu—Kor light source (XRPD4—3).

These peaks may be those having a relative intensity of about 20% or more.

The above crystalline form (ex.4) may have an endothermic peak which has a starting point at about 50°C and its lowest point at about 73°C, an ermic peak at about 189°C, and an endothermic peak at about 222°C in a DSC (10°C /min).

The above crystalline form (ex.4) may have a water content of about 8.9% (theoretical water content value of 8.8%) and a melting point of about 210°C to 215°C.

The above crystalline form (ex.4) shows a hygroscopicity increase of about 6% in the region with a relative humidity of 10% to 30%, but the hygroscopicity in the region with a relative humidity of 40% or higher may be measured at a very low and nt level.

The above crystalline form (ex.4) may have a 13C CP/MAS TOSS ssNMR spectrum comprising peaks at the following 13C chemical shifts: 43.8 i 0.2 ppm and 53.8 i- 0.2 ppm (ssNMR4-1).

More specifically, the above crystalline form (ex.4) may have a 13C CP/MAS TOSS ssNMR spectrum sing peaks at the following 13C chemical shifts: 43.8 i 0.2 ppm, 46.7 i 0.2 ppm, 49.9 i 0.2 ppm and 53.8 i 0.2 ppm (ssNMR4—2).

The above crystalline form (ex.4) may have a 13C CP/MAS TOSS ssNMR spectrum comprising peaks at the ing 13C chemical shifts: 117.7 i 0.2 ppm, 153.1 i 0.2 ppm and 165.6 i 0.2 ppm (ssNMR4—3).

More specifically, the above crystalline form (ex.4) may have a 13C CP/MAS TOSS ssNMR spectrum comprising peaks at the following l3C al : 117.7 i 0.2 ppm, 120.6 i 0.2 ppm, 130.0 i 0.2 ppm, 147.6 i 0.2 ppm, 153.1 i 0.2 ppm and 165.6 i 0.2 ppm (ssNMR4—4).

More specifically, the above crystalline form (ex.4) may have a 13C CP/MAS TOSS ssNMR spectrum comprising peaks at the following l3C chemical : 43.8 i 0.2 ppm, 53.8 i 0.2 ppm, 117.7 i 0.2 ppm, 153.1i 0.2 ppm and 165.6 1 0.2 ppm (ssNMR4—5).

The above crystalline form (ex.4) may have (a) an X-ray powder diffraction (XRPD) pattern comprising peaks at diffraction angle 20 values of 6.4°i 0.20, 7.0°i 0.20, 12.8% 0.20 and 21.0°i 0.20 when irradiated with a Cu-Kor light source; and (b) a 13C solid state NMR um comprising peaks at the following 13C chemical shifts: 43.8 i 0.2 ppm and 53.8 i 0.2 ppm.

The above crystalline form (ex.4) may have (a) an X—ray powder diffraction (XRPD) pattern comprising peaks at diffraction angle 20 values of 6.4°i 0.20, 7.0°i 0.20, 12.8°i 0.2° and 21.0°i 0.20 when irradiated with a Cu—Kor light source; and (b) a 13C solid state NMR spectrum comprising peaks at the following 13C chemical shifts: 117.7 i 0.2 ppm, _r 0.2 ppm and 165.6 i 0.2 ppm.

The above crystalline form (ex.4) may also be characterized by any other com— bination of lists of XRPD peaks (XRPD4—1 to XRPD4—3) and 13C chemical shifts (ssNMR4—1 to ssNMR4—5) as listed above.

In another exemplary embodiment (ex.5), the present invention also provides a crystalline form of a monohydrochloride e, preferably monohydrate (lHCl-IHZ O), of the compound of Formula 1.

This crystalline form (ex.5) exhibits an XRPD pattern comprising peaks at diffraction angles of 20 = 7.8°i0.2°, 0.2O and 25.7°i0.2O when irradiated with a Cu-KOL light source.

More specifically, the above crystalline form (ex.5) has an XRPD pattern comprising peaks at diffraction angles of 20 = 78010.2", 10.70102", 13.00i0.2°, 186010.20, 19.1°4_-0.2°, 22.0°iO.2°, 22.5°i-0.2°, 24.6°4_-0.2°, 25.3°iO.2°, and 25.7°i0.2O when irradiated with a Cu—Ka light source (XRPD5—2).

More specifically, the above lline form (ex.5) has an XRPD pattern comprising peaks at diffraction angles of 20 = 7.8°i0.2°, 0.2°, 12.7°i0.2°, 13.0°i0.2°, O.2°, 17.7°iO.2°, 18.6°iO.2°, O.2°, 21.5°i0.2°, 22.0°iO.2°, 22.5°iO.2°, 24.6°i0.2°, 25 .3°i0.2°, and 25.7°i0.2° when irradiated with a Cu—Ka light source (XRPD5—3).

These peaks may be those having a relative intensity of about 20% or more.

The above lline form(ex.5) may have an endothermic peak which has a starting point at about 115°C and its lowest point at about 142°C, an exothermic peak at about 204°C, and an endothermic peak which has a starting point at about 210°C and its lowest point at about 251°C, in a DSC (10°C /min).

The above crystalline form (ex.5) may have a water content of about 3.5% (theoretical water content value of 3.33%) and a melting point of about 190°C to 200°C.

The above crystalline form (ex.5) shows a hygroscopicity measured at a very low level in the region with a relative humidity of 10% to 70% but the hygroscopicity in the region with a relative humidity of 70% or higher may be ed to be about 7%.

The above crystalline form (ex.5) may have a 13C CP/MAS TOSS ssNMR spectrum comprising peaks at the following 13C chemical shifts: 42.5 i 0.2 ppm and 54.4 i 0.2 ppm (ssNMR5— 1).

More specifically, the above crystalline form (ex.5) may have a 13C CP/MAS TOSS ssNMR spectrum comprising peaks at the following l3C chemical shifts: 42.5 i 0.2 ppm, 45.4 i 0.2 ppm, 51.0 i 0.2 ppm and 54.4 i 0.2 ppm (ssNMR5—2).

The above crystalline form (ex.5) may have a 13C CP/MAS TOSS ssNMR spectrum sing peaks at the following 13C chemical shifts: 124.1 1 0.2 ppm, 131.8 i 0.2 ppm and 164.7 1- 0.2 ppm (ssNMR5—3).

More specifically, the above crystalline form (ex.5) may have a 13C CP/MAS TOSS ssNMR spectrum comprising peaks at the following l3C chemical shifts: 114.8 i 0.2 ppm, 124.1 i 0.2 ppm, 129.3 i 0.2 ppm, 131.8 i 0.2 ppm, 153.5 i 0.2 ppm and 164.7 i 0.2 ppm (ssNMR5—4).

More specifically, the above crystalline form (ex.5) may have a 13C CP/MAS TOSS ssNMR spectrum comprising peaks at the following l3C chemical shifts: 42.5 i 0.2 ppm, 45.4 i 0.2 ppm, 51.0 i- 0.2 ppm, 54.4 i- 0.2 ppm, 114.8 1 0.2 ppm, 124.1 4.- 0.2 ppm, 129.3 i 0.2 ppm, 131.8 i 0.2 ppm, 153.5 i 0.2 ppm and 164.7 i 0.2 ppm (ssNMR5—5).

The above crystalline form (ex.5) may have (a) an X-ray powder diffraction (XRPD) n comprising peaks at diffraction angle 26 values of 7.8°«_L 0.2°, 22.5°i- 0.2° and 25.7°w_L 0.2° when ated with a Cu- KOL light source; and (b) a 13C solid state NMR um comprising peaks at the following 13C chemical shifts: 42.5 i 0.2 ppm and 54.4 i 0.2 ppm.

The above crystalline form (ex.5) may have (a) an X—ray powder diffraction (XRPD) pattern comprising peaks at diffraction angle 26 values of 7.8°i 0.2°, 225°: 0.2° and 257°: 0.2° when irradiated with a Cu— K0t light ; and (b) a 13C solid state NMR spectrum sing peaks at the following l3C chemical shifts: 117.7 i 0.2 ppm, 153.1 i 0.2 ppm and 165.6 i 0.2 ppm.

The above crystalline form (ex.5) may also be characterized by any other com— bination of lists of XRPD peaks (XRPDS—l to XRPD5—3) and 13C chemical shifts (ssNMRS-l to ssNMRS-S) as listed above.

In another ary embodiment (ex.6), the t invention also provides a crystalline form (Type A) of a monohydrochloride hydrate, preferably dihydrate (1HCl-2H20), of the compound of Formula 1.

This crystalline form (ex.6) exhibits an XRPD n comprising peaks at diffraction angles of 26 = 7.5°i0.2°, O.2° and 20.0°i0.2° when irradiated with a Cu—Koz light source (XRPD6—1).

More specifically, the above crystalline form (ex.6) has an XRPD pattern comprising peaks at diffraction angles of 26 = .2°, 15.1°i0.2°, 20.0°i0.2°, 21.2°i0.2°, and .1°i0.2° when irradiated with a Cu—Kor light source (XRPD6—2).

More specifically, the above crystalline form (ex.6) has an XRPD pattern comprising peaks at diffraction angles of 26 = 6.8°i0.2°, 7.5°i0.2°, 15.1°i0.2°, 17.0°i0.2°, 18.1°i0.2°, 20.0°i0.2°, 21.2°i0.2°, 22.7°i0.2°, 23.0°i0.2°, 25.1°i0.2°, and 26.5°i0.2° when irradiated with a Cu—Kor light source (XRPD6—3).

These peaks may be those having a relative intensity of about 10% or more.

The above crystalline form (ex.6) may have an endothermic peak which has a starting point at about 62°C and its lowest point at about 90°C, and an endothermic peak which has a starting point at about 171°C and its lowest point at about 182°C, in a DSC (10°C /min).

The above crystalline form (ex.6) may have a water content of about 6.8% (theoretical water content value of 6.45%) and a g point of about 190°C to 200°C.

The hygroscopicity of the above crystalline form (ex.6) in the region with a relative ty of 10% to 90% may be measured to be about 2%, in a DVS.

The above crystalline form (ex.6) may have a 13C CP/MAS TOSS ssNMR spectrum comprising peaks at the following 13C chemical shifts: 43.1 i 0.2 ppm and 53.2 i- 0.2 ppm (ssNMR6-1).

More specifically, the above crystalline form (ex.6) may have a 13C CP/MAS TOSS ssNMR spectrum comprising peaks at the following 13C chemical shifts: 43.1 i 0.2 ppm, 46.5 i 0.2 ppm, 48.1 i 0.2 ppm and 53.2 i 0.2 ppm 6—2).

The above crystalline form (ex.6) may have a 13C CP/MAS TOSS ssNMR spectrum comprising peaks at the following 13C chemical shifts: 117.6 i 0.2 ppm, 133.4 i 0.2 ppm and 164.3 i 0.2 ppm (ssNMR6—3).

More specifically, the above lline form (ex.6) may have a 13C CP/MAS TOSS ssNMR spectrum comprising peaks at the following l3C chemical shifts: 117.6 i 0.2 ppm, 133.4 i 0.2 ppm, 137.8 i 0.2 ppm, 151.7 i 0.2 ppm, 164.3 i 0.2 ppm and 165.0 i 0.2 ppm (ssNMR6—4).

More specifically, the above crystalline form (ex.6) may have a 13C CP/MAS TOSS ssNMR um sing peaks at the following l3C chemical shifts: 43.1 i 0.2 ppm, 46.5 i 0.2 ppm, 48.1 i 0.2 ppm, 53.2 i 0.2 ppm, 117.6 i 0.2 ppm, 133.4 i 0.2 ppm, 137.8 i 0.2 ppm, 151.7 i 0.2 ppm, 164.3 i 0.2 ppm and 165.0 i 0.2 ppm (ssNMR6—5).

The above crystalline form (ex.6) may have (a) an X—ray powder diffraction (XRPD) pattern comprising peaks at diffraction angle 20 values of 7.5% 0.20, 15.10: 02" and 20.0% 0.20 when irradiated with a Cu— Koz light source; and (b) a 13C solid state NMR spectrum comprising peaks at the following 13C chemical shifts: 43.1 i 0.2 ppm and 53.2 i 0.2 ppm.

The above crystalline form (ex.6) may have (a) an X—ray powder diffraction (XRPD) pattern comprising peaks at ction angle 20 values of 7.501 0.20, 15.1°i 0.20 and 20.0% 0.20 when irradiated with a Cu— Koz light source; and (b) a 13C solid state NMR spectrum comprising peaks at the following 13C chemical shifts: 117.6 i 0.2 ppm, 133.4 i 0.2 ppm and 164.3 i 0.2 ppm.

The above crystalline form (ex.6) may also be characterized by any other com— bination of lists of XRPD peaks (XRPD6—1 to XRPD6—3) and 13C chemical shifts (ssNMR6—1 to -5) as listed above.

In r exemplary embodiment (ex.7), the present invention es a crystalline form (Type B) of a monohydrochloride hydrate, preferably dihydrate (1HCl-2H20), of the compound of Formula 1.

This crystalline form (ex.7) exhibits an XRPD pattern sing peaks at diffraction angles of 20 = 8.7°i0.2°, 19.4"w_L0.2O and 23.1°iO.2° when irradiated with a Cu—Koz light source (XRPD7—1).

More specifically, the above crystalline form (ex.7) has an XRPD pattern comprising 2016/015535 peaks at diffraction angles of 20 = 8.7°iO.2°, 11.6°i-0.2°, 17.5°i0.2°, 19.4°iO.2° 0.2°, and 26.1°i0.2° when irradiated with a Cu—Ka light source (XRPD7—2).

More specifically, the above lline form (ex.7) has an XRPD pattern comprising peaks at ction angles of 20 = 8.7°i 0.2°, 11.6°i 0.2°, 14.4°i 0.2°, 17.5°i 0.2°, 194°: 0.2°, 20.8°i 0.2°, 21.9°i 0.2°, 231°: 0.2°, 261°: 0.2° and 280°: 0.2° when irradiated with a Cu—Ka light source.

These peaks may be those having a relative intensity of about 20% or more.

The above crystalline form (ex.7) may have an endothermic peak which has a ng point at about 55°C and its lowest point at about 71°C, and an endothermic peak which has a starting point at about 215°C and its lowest point at about 222°C, in a DSC (10°C /min).

The above crystalline form (ex.7) may have a water content of about 6.0% (theoretical water content value of 6.45%) and a melting point of about 190°C to 200°C.

The hygroscopicity of the above crystalline form (ex.7) in the region with a relative humidity of 10% to 90% may be measured to be about 14%, in a DVS.

The above crystalline form (ex.7) may have a 13C CP/MAS TOSS ssNMR spectrum comprising peaks at the following 13C chemical shifts: 41.3 i 0.2 ppm, 48.8 i 0.2 ppm and 55.9 i 0.2 ppm (ssNMR7—l).

More specifically, the above crystalline form (ex.7) may have a 13C CP/MAS TOSS ssNMR spectrum comprising peaks at the following l3C chemical shifts: 41.3 i 0.2 ppm, 42.6 i 0.2 ppm, 48.8 i 0.2 ppm, 50.0 i 0.2 ppm and 55.9 i 0.2 ppm (ssNMR7-2).

The above crystalline form (ex.7) may have a 13C CP/MAS TOSS ssNMR spectrum comprising peaks at the ing 13C chemical shifts: 119.0 i 0.2 ppm, 152.7 i 0.2 ppm and 165.0 i 0.2 ppm (ssNMR7—3).

More specifically, the above crystalline form (ex.7) may have a 13C CP/MAS TOSS ssNMR spectrum comprising peaks at the ing l3C chemical shifts: 119.0 i 0.2 ppm, 132.9 i 0.2 ppm, 139.0 i 0.2 ppm, 152.7 i 0.2 ppm, 163.5 i 0.2 ppm and 165.0 i 0.2 ppm (ssNMR7—4).

More specifically, the above crystalline form (ex.7) may have a 13C CP/MAS TOSS ssNMR spectrum comprising peaks at the following l3C chemical shifts: 41.3 i 0.2 ppm, 42.6 i 0.2 ppm, 48.8 i 0.2 ppm, 50.0 i 0.2 ppm, 55.9 i 0.2 ppm, 119.0 i 0.2 ppm, 132.9 i 0.2 ppm, 139.0 4.- 0.2 ppm, 152.7 1 0.2 ppm, 1635 i- 0.2 ppm and 165.0 i 0.2 ppm 7—5).

The above crystalline form (ex.7) may have (a) an X—ray powder diffraction (XRPD) pattern comprising peaks at diffraction angle 20 values of 87°;L 0.2°, l9.4°4_- 0.2° and 23.1°w_L 0.2° when irradiated with a Cu- Koz light source; and (b) a 13C solid state NMR spectrum comprising peaks at the following 13C chemical : 41.3 i 0.2 ppm, 488 i 0.2 ppm and 55.9 i 0.2 ppm.

The above crystalline form (ex.7) may have (a) an X-ray powder diffraction (XRPD) pattern comprising peaks at diffraction angle 20 values of 8.7°i 0.20, l9.4°i 0.20 and 23.l°i 0.20 when irradiated with a Cu— Ka light source; and (b) a 13C solid state NMR spectrum comprising peaks at the following 13C chemical shifts: 1190 i 0.2 ppm, 152.7 i 0.2 ppm and 165.0 i 0.2 ppm.

The above crystalline form (ex.7) may also be characterized by any other com— bination of lists of XRPD peaks (XRPD7—1 to 3) and 13C chemical shifts (ssNMR7—l to ssNMR7—5) as listed above.

Medical use and ceutical composition As disclosed in , the compound of Formula 1 has been shown to be useful for the selective and effective inhibitory ty against the growth of cancer cells induced by a mutation in epidermal growth factor receptor (EGFR) tyrosine kinase, and drug resistance thereof.

In one aspect the invention further provides a hydrochloride salt of the compound of a 1 or a crystalline form of hydrochloride salt of the compound of Formula 1 as described herein for use in the treatment of a cancer harboring one or more EGFR In a further aspect the ion provides a method for the treatment of cancer comprising administering to a patient in need thereof a therapeutically effective amount of a hydrochloride salt of the compound of Formula 1 or a crystalline form of a hydrochloride salt of the nd of Formula 1 as described herein, wherein the cancer to be treated is a cancer harboring one or more EGFR mutation.

In a further aspect the cancer to be treated is a cancer harboring one or more EGFR mutations wherein at least one EGFR on is ed from Dell9 (deletion in exon 19), L858R and T790M.

In a further aspect the cancer to be treated is a cancer harboring a Dell9 EGFR mutation.

In a further aspect the cancer to be treated is a cancer harboring the EGFR mutation L858R.

In a r aspect the cancer to be treated is a cancer harboring the EGFR mutation T790M.

In a further aspect the cancer to be treated is a cancer harboring at least two EGFR mutations selected from the group consisting of De119/T79OM and L858RfT79OM.

In this aspect, the hydrochloride salt of the compound of Formula 1 or a crystalline form of the hydrochloride salt of the compound of Formula 1 may be used for the preparation of a ceutical ition for preventing or treating cancers or tumors induced by epidermal growth factor receptor tyrosine kinase or a mutant thereof. The pharmaceutical composition may be used to treat the same cancers harboring EGFR mutation as bed for the hydrochloride or lline forms of the hloride hereinbefore.

Accordingly, the t invention provides a pharmaceutical composition containing a hloride salt of the compound of Formula 1, preferably in crystalline form, and at least one pharmaceutically acceptable carrier or t. The pharma— ceutical ition may be used for the treatment of cancers or tumors induced by epidermal growth factor receptor tyrosine kinase or a mutant thereof.

The administration dose of the hydrochloride salt of the compound of Formula 1, preferably in crystalline form or a pharmaceutical composition containing the same may vary depending on the subject to be treated, severity of illness or health state of the subject, administration rate, physician's decision, etc, but it may be conventionally stered to a human subject having a body weight of e.g. 70 kg via an oral or parenteral administration route in an amount of from 10 mg to 2,000 mg as a free base based on the compound of Formula 1, preferably in an amount of 50 mg to 1,000 mg, 1 to 4 times daily or on an on/off schedule. In some cases, it may be more riate to ster a lower dosage than that mentioned above, a higher dosage than the above may be administered if it does not cause harmful side effects, and in the case when a significantly larger dosage is to be administered, the administration may be performed daily by several divided doses with a lesser dosage per stration.

The ceutical composition according to the present invention may be prepared in various formulations for oral administration ing to the conventional methods, e.g., tablets, pills, powders, capsules, syrups, emulsions, microemulsions, etc, or for parenteral administration, e.g., intramuscular, intravenous, or subcutaneous adminis— trations.

The pharmaceutical composition may contain any conventional non—toxic, pharma— ceutically acceptable carrier, diluent, nt, excipient, or vehicle. When the phar— maceutical composition according to the present invention is ed as a formulation for oral administration, the r to be used may include, e.g., cellulose, calcium silicate, corn starch, lactose, sucrose, dextrose, calcium phosphate, stearic acid, magnesium stearate, calcium stearate, gelatin, talc, surfactant, suspending agents, emulsifying agents, diluents, etc. Additionally, when the pharmaceutical composition is prepared as a formulation for oral administration, the diluents to be used may include lactose, mannitol, saccharide, microcrystalline cellulose, cellulose derivative, corn , etc. When the pharmaceutical composition according to the present ion is prepared as a ation for injections, the carrier to be used may include, e.g., water, saline, an aqueous glucose solution, an aqueous sugar-like solution, alcohols, glycols (e. g., polyethylene glycol 400), ethers, oils, fatty acids, fatty acid esters, ides, surfactants, suspending agents, emulsifying agents, etc.

Hereinafter, the present invention will be described in more detail with nce to the following Examples. r, these Examples are for illustrative purposes only, and the invention is not intended to be limited by these Examples.

Analysis Apparatus and Method of Measurement 1. X-ray Powder ction (XRPD) X-ray powder diffraction (XRPD) analyses of samples were performed in the range from 3°20 to 40°20 using a D8 e (Bruker ASX, Germany) analyzer. When the amount of a given sample was less than 100 mg, about 5 mg to 10 mg of the sample was gently compressed on a glass slide which was fit into a sample holder. When the amount of a given sample was greater than 100 mg, about 100 mg of the sample was gently compressed in a plastic sample holder so that the sample surface becomes flat and positioned immediately on top of the sample holder level.

The measurement was performed as follows: Anode material (KOL): Cu ch (1.54056 A) Scan range: 3° to 40° Generator settings: 100 mA, 40.0 kV Scan speed: 1 sec/step Diver slit: 0.3° Anti—scatter slit: 0.3° Temperature: 20°C Step size: 002° 20 Rotation: use Goniometer radius: 435 mm 2. Differential Scanning Calorimeter (DSC) Differential scanning calorimeter (DSC) is was performed in as STA—1000 (Scinco, Korea) at 30°C to 350°C. A sample in an amount of 5 mg to 10 mg was weighed and added into an aluminum DSC fan, and the fan was sealed with a perforated aluminum lid in a non— sealing manner. Then, the sample was heated at a scan speed of 10°C /min from 30°C to 350°C, and the heat flow reaction generated was red in a DSC. 3. Dynamic Vapor Sorption (DVS) Dynamic vapor on (DVS) analysis was performed in a DVS advantage (Sulface measurement system, United Kingdom) analyzer at 25°C with a relative humidity of 0% to 90%. A sample in an amount of 10 mg was placed into a wire-mesh vapor sorption balance pan and then attached to a DVS advantage dynamic vapor sorption e via surface measurement systems. Until a stable weight was achieved (99.5% completion of steps), the sample was applied to a ramping e with a relative humidity of 10% to 90% with a 10% increase of the sample while maintaining the sample in each step. Upon completion of the sorption cycle, the sample was dried using the same s while maintaining a relative ty of below 0%. The changes in the sample weight during the adsorption/desorption cycle (repeated 3 times) were ed and the hygroscopicity of the sample was measured. 4. Solid State Nuclear Magnetic Resonance Spectroscopy (ssNMR) Solid State Nuclear Magnetic Resonance Spectroscopy (ssNMR) was performed for the purpose of comparing of the polymorphs by NMR oscopy in the solid state.

A sample in an amount of 100 mg was weighed and added into a 4 mm sample tube. 13 C NMR spectra (13C CP/MAS TOSS ssNMR) were recorded at room temperature using a Bruker Avance II 500 MHz Solid NMR system (Bruker, Germany) analyzer with 4 mm probe type CP/MAS BB— 1H under the following conditions: Frequency: 125.76 MHz, Spectral width: 20 kHz, Rotational speed of the sample at the magic angle: 5 kHz, Pulse Sequence: CP (Cross Polarization) SPINAL64 with decoupling (decoupling power of 80 kHz), Delay repeats; 5 s Contact time: 2 ms Number of scans: 4096.

External standard: tane . High Performance Liquid Chromatography (HPLC) High performance liquid chromatography (HPLC) analysis was performed for the e of analyzing purity and contents such as stability test, etc, using an Agilent 1100/1200 series HPLC s (Agilent, USA) analyzer, and the conditions used for HPLC are as follows.

Purity and content analysis conditions: thienopyrimidine compound of Formula 1 Column: Hydrosphere C18 (YMC), 5 um (150 mm x 4.6 mm) Column temperature: 30°C Detector: UV spectrophotometer Detection wavelength: 254 nm Flow rate: 1.0 mL/min Time of analysis: 35 min Eluent: NaClO4-NaH2PO4 - phosphate buffer solution (pH 2.5 4.- 0.1)/CH3CN = 40/60 (v/v%) 6. Ion Chromatography (IC) Ion chromatography (IC) analysis was performed for the purpose of analyzing the hydrochloric acid content in a hydrochloride salt using a Thermo Fisher ific ICS— 2500 series IC Systems (Thermo Fisher Scientific, USA) analyzer, and the conditions used for IC analysis are as follows.

Conditions for content analysis: thienopyrimidine compound of Formula 1 Column: IonPac AS19 (Dionex), (250 mm x 4 mm), guard (50 mm x 4 mm) Column temperature: 30°C Detector: Conductivity detector (CD) Suppressor: ASRS 4 mm, current 40 mA Flow rate: 1.0 mL/min Time of is: 30 min Eluent: 10 mM KOH solution 7. Measurement of water content Water content was measured using a 795KFT Titrino (Metrohm, Switzerland) Karl Fischer titrator. 8. Measurement of melting point Melting point was measured using an IA9200 (Electrothermal, UK) melting point measuring device.

Examples: Preparation of a crystalline form of a hydrochloride salt of a compound of a 1 Example 1. Preparation of a crystalline form (Type A) of a dihydrochloride e, preferably monohydrate 1H20), of a compound of Formula 1 A compound of Formula 1 prepared according to the method disclosed in WO 62515 nced herein or a similar method f, as referenced herein, in an amount of 10.0 g was added into 100 mL of a 90% aqueous ethanol solution (ethanol/water = 9/ 1). A concentrated HCl solution in an amount of 4 mL (45.2 mmol) was added thereto, stirred at room temperature for 6 hours, and the ing pre— cipitated solids were filtered. The resultant was washed with 20 mL of a 90% aqueous ethanol solution ol/water = 9/ 1) and dried to obtain 9.0 g of the title compound (yield: 800%).

Water content: 3.1% (theoretical value for a monohydrate: 3.11%) Ion chromatography: 13.1% (theoretical value for a dihydrochloride: 13.0%) In a further aspect the invention provides a crystalline form of a hydrochloride salt of the compound of Formula 1 prepared by a process comprising the steps of WO 16192 (a) adding an aqueous solution of an alcohol to the free base of the compound of Formula 1; (b) adding 1.5 to 3 eq. of HCl to the mixture obtained in step (a) (in relation to the free base); and (C) collecting the resulting precipitate.

Preferably, the free base used in this process is in amorphous form. Preferably the aqueous solution of an alcohol used in this s is an aqueous solution of ethanol or iso—propanol, more preferably the aqueous solution is a 85—95% s solution of ethanol or opanol, 90 % is especially red. Most preferred is a 90% aqueous solution of ethanol. The preferred amount of HCl added is in the range of 2 to 2.5 eq. of HCl (in relation to the free base), most preferred is 2.2 to 2.3 eq. of HCl. Preferably, the mixture obtained after adding HCl is stirred a room temperature for 5 to 8 hours (6 hours are preferred). Collection of the precipitate can be achieved by filtration. Optionally , after collection the precipitate can be washed with the same aqueous solution of an alcohol as used in step (a) of the process.

Analysis of characteristics The results of XRPD, DSC, DVS and ssNMR analyses of the crystalline form ed in Example 1 are shown in FIGS. 1A, 2A, 3A and 4A, respectively.

The peaks having a relative ity (I/Io) of 3% or higher in the XRPD spectrum of the above crystalline form are shown in Table 1 below. When the peaks had I/Io ratios equal to or higher than 10%, they appeared at diffraction angles of 56°, 10.7°, ll.l°, 14.00, 20.80, 2l.l°, 22.50, and 27.30 (20 i 02°).

[Table l] WO 16192 20 ($0.2) (1 1/10 (Va) 20 (3:01) d lilo (%) .6 15.9 100 22.5 4.0 10.2 8.9 9.9 6.7 23.2 3.8 9.0 .7 8.3 14.2 24.1 3.7 6.5 11.1 7.9 19.1 24.7 3.6 3.6 11.4 7.7 6.1 25.0 3.6 8.8 12.2 7.3 5.1 26.4 3.4 6.5 14.0 6.3 15.6 26.8 3.3 4.5 14.6 6.1 9.3 27.3 3.3 24.7 .5 5.7 4.8 27.7 3.2 3.6 .7 5.6 5.0 28.8 3.1 6.5 16.8 5.3 3.0 29.4 3.0 6.5 18.5 4.8 3.3 29.8 3.0 5.0 18.9 4.7 6.2 30.1 3.0 4.4 19.9 4.5 6.9 30.6 2.9 3.0 ' ' ' ' ' ' ' .4 4.3 7.8 31.3 2.9 '4.0 .8 4.3 20.0 32.4 2.8 4.4 21.1 4.2 41.6 34.7 2.6 3.5 21.4 4.1 3.0 37.9 2.4 3.3 21.7 4.] 4.5 3.9.7 2.3 3.0 26: diffraction angle, d: distance between l faces, 1/10 (%)2 relative intensity (1 indicates the intensity of each peak; 10 indicates the intensity of the highest peak.) In applying the conditions of measurement as disclosed herein the above crystalline form showed a broad endothermic peak between 25-150°C associated with dehydration and an endotherm with a peak temperature about 238°C which is associated with melting and decomposition.

The above crystalline form showed a water content of about 3.1% (theoretical water content value: 3.11%) in a Karl Fischer titrator and a melting point from about 202°C to about 225°C.

In the DVS for the above crystalline form, the level of water tion measured in the region with a relative humidity of 10% to 90% was very low (2—3%) and ible.

The above crystalline form was shown to be fully stable under a long—term storage condition (e. g., a temperature of 25°C and a relative humidity of 60%), an accelerated condition (e. g., a temperature of 40°C and a relative humidity of 75%), and a stress testing condition (e.g. , a temperature of 60°C).

In the ssNMR spectroscopy for the above lline form, the observed peaks were collected in the Table 2 below (expressed in ppm i 0.2 ppm): [Table 2] Chemical Sllifl Chemical Shift al Shift (ppm) (ppm) (ppm) 1 44.6 8 116.5 1 15 144.2 45.4 119.] 146.8 Main: 7 114.4 [4 140.4 l‘i'] Example 2. Preparation of a crystalline form (Type B) of a dihydrochloride hydrate, preferably monohydrate (2HCl-1H20), of a compound of Formula 1 The dihydrochloride hydrate, preferably trihydrate, (Type B) of a nd of Formula 1, prepared in Example 4 to be described later, in an amount of 1.0 g was dried in a chamber for stability testing at 60°C for one week to obtain 1.0 g of the title compound. [295 Water content: 3.3% (theoretical value for a monohydrate: 3.1%) [296 Ion chromatography: 12.8% (theoretical value for a dihydrochloride: 13.0%) [297 [298l—Jl—ll—Jl—l Analysis of characteristics The result of XRPD is of the crystalline form prepared in Example 2 is shown in .

The peaks having a ve intensity (I/Io) of 3% or higher in the XRPD spectrum of the above crystalline form are shown in Table 3 below. When the peaks had I/Io ratios equal to or higher than 10%, they appeared at diffraction angles of 64°, 81°, 97°, 128°, 137°, 143°, 160°, 190°, 208°, 212°, 220°, 24.1°, 24.6°, 249°, 260°, 263°, 268°, 27.1°, 281°, 292°, 309°, and 344° (20 i 0.2°).

[Table 3] 20 (20.2) 171.1%) 20 120.2) 14.1"») 3.2 27.3 1 7.9 22.0 4.0 78.3 3.4 26.1 ‘ (3.6 23.4 3.8 5.7 1 6.4 13.9 1 59.6 24.1 3.7 42.6 7.3 12.0 1 3 24.6 3.6 17.2 8.1 10.8 46.9 24.9 3.6 14.2 9.7 9.1 31.3 25.4 3.5 6.2 “W102 81'6"" ......... . .. y _ 25.6 3‘5 9.3. 6.6 8.4 3.3 22 [3.7 (3.4 1 10.4 2-7.1 3.3 35.3 14.3 6.2 13.6 28.1 3.2 24.5 14.7 6.0 8.7 28.6 3.1 5.5 .8 8.1 29.2 3.1 16.3 . 1 2.1, 19.6 4.5 1 8.8 15 4 2.5 7.3 .8 4.3 1 100 2.4 5.8 21.2 4.2 1 15.2 39.5 2.3 7.6 21.6 4.1 1 8.5 '20: diffraction angle, d: distance between crystal faces, [1’I;1(%): relative intensity (1 indicates the ity of each peak; 10 tes the intensity of the highest Epeak.) In the ssNMR spectroscopy for the above crystalline form, the ed peaks were collected in the Table 4 below ssed in ppm i 0.2 ppm): [Table 4] Chemical Shifl 1 Chemical Shift Chemical Shift Peak# 1 Peak# Peak# (ppm) 1 (Wm) (ppm) 1 1 43.4 8 1 115.0 15 142.4 2 1 45.2 9 1 117.0 113 147.1 3 1 49.8 10 1 120.4 17 149.8 4 51.3 11 1 128.7 18 151.7 33.3 12 1 131.1 19 165.2 6 1 109.6 13 1 135.1 . 7 1 113.0 14 1 138.6 — Example 3. Preparation of a lline form (Type A) of a dihydrochloride hydrate, preferably trihydrate (2HC1~3H20), of a compound of Formula 1 The dihydrochloride hydrate, preferably monohydrate, (Type A) of a compound of Formula 1 (Example 1) in an amount of 10.0 g was added into 100 mL of water. The mixture was heated under reflux, stirred for 30 minutes, cooled to room temperature, and stirred for 12 hours, and the resulting precipitated solids were filtered. The ed precipitate was washed with 20 mL of water and dried to obtain 8.0 g of the title compound (yield: 80.0%). [307 Water content: 10.1% (theoretical value for a monohydrate: 8.8%) [308 Ion chromatography: 11.1% (theoretical value for a monohydrochloride: 13.0%) [309 Analysis of characteristics [310Hi—JHI—J The results of XRPD, DSC, DVS and ssNMR analyses of the crystalline form prepared in Example 3 are shown in FIGS. 1C, 2B, 3B and 4B, respectively.

The peaks having a relative intensity (I/Io) of 3% or higher in the XRPD spectrum of the above crystalline form are shown in Table 5 below. When the peaks had I/Io ratios equal to or higher than 10%, they appeared at ction angles of 46°, 86°, 15 .1°, 158°, 172°, 179°, 185°, 197°, 201°, 211°, 213°, 230°, 235°, 244°, 24.7°, 251°, 258°, 263°, 26.8°, 27.8°, and 284° (20 i 0.2°).

[Table 5] 29 ($0.2) d m, (0,4,) 29 (10.2) d 1mm.) 4.6 19.0 100 21.3 4.2 14.3 6.9 12.8 3.5 22.3 4.0 5.8 7.6 11.7 9.8 23.0 3.9 14.9 7.9 11.2 8.7 23.5 3.8 16.9 8.6 10.2 62.1 24.4 3.6 12.2 9.6 9.2. 9.8 24.7 3.6 11.3 12.9 6.9 5.8 25.1 3.5 26.1 ....... 133 (1'7”- .......38 2'51; 3-4 12-5 13.9 6.4 3.8 2.6.3 3.4 33.2 14.3 6.2 4.8 26.8 3.3 13.2 .1 5.8 14.4 27.8 3.2 13.2 .8 5.6 50.8 28.4 3.1 11.8 17.0 5.2 9.3 28.9 3.1 8.0 17.2 5.1 24.6 30.4 2.9 9.4 17.9 4.9 10.1 31.1 2.9 4.3 18.5 4.8 12.7 31.7 2.8 7.0 19.2 4.6 7.2 32.7 2.7 7.6 19.7 4.5 20.9 33.8 2.7 4.7 .1 4.4 15.8 34.5 2.6 6.5 21.1.5 4.3 8.3 37.1 2.4 6.9 21.1 4.2 15.1 38.1 2.4 6.1 : diffraction angle, d: distance n cryslal faces, 1/10 ('96): vc intensity (1 indicates the intensity of each peak; 1¢indicatcs the intensity of the highest peak.) In applying the conditions of measurement as disclosed herein the above crystalline form showed endothermic peaks at about 51°C, about 95°C, and about 178°C, and an endothermic peak at about 218°C in a DSC (10°C /min). In the DSC, the endothermic peaks at about 51°C, about 95°C, and about 178°C indicate the dehydration point of the crystalline form of the dihydrochloride trihydrate, and the endothermic peak at about 218°C indicates a melting point.

The above crystalline form showed a water t of about 10.1% (theoretical water content value: 8.8%) in 21 Karl Fischer titrator and a melting point from about 205°C to about 210°C.

In the DVS for the above crystalline form, the level of water absorption in the region with a ve humidity of 10% to 40% was measured at a very low level, r, the level of water absorption in the region with a relative humidity of 40% or higher was measured at a higher level of about 9%. The above crystalline form was expected to maintain the crystalline form of the trihydrate due to absorption of water under a long—term storage condition (e.g., a temperature of 25°C and a relative humidity of 60%) and an accelerated condition (e.g., a temperature of 40°C and a relative humidity of 75%).

In the ssNMR spectroscopy for the above crystalline form, the observed peaks were collected in the Table 6 below (expressed in ppm i 0.2 ppm): [Table 6] Chemical Slnfl Chemical Sluft Chemical Slnfi Peak # : Peak ff Peak {1 (ppm) (Wm) (Wm) 1 42.7 8 1 115.7 l5 150.2 : ' 45.0 131,4 152.0 Example 4. Preparation of a crystalline form (Type B) of a dihydrochloride hydrate, ably trihydrate (2HCl~3H20), of a compound of a 1 A dihydrochloride hydrate, preferably monohydrate, (Type A) of a compound of Formula 1 (Example 1) in an amount of 10.0 g was added into 100 mL of a 70% aqueous ethanol solution (ethanol/water = 9/1). The e was heated under reflux, stirred for 30 minutes, cooled to room temperature, and d for 12 hours, and the resulting itated solids were filtered. The filtered precipitate was washed with 20 mL of the same t and dried to obtain 7.0 g of the title compound (yield: 70.0%).

Water content: 8.9% etical value for a trihydrate: 8.8%) Ion chromatography: 13.0% (theoretical value for a dihydrochloride: 13.0%) Analysis of characteristics The results of XRPD, DSC, DVS and ssNMR analyses of the crystalline form prepared in Example 4 are shown in FIGS. 1D, 2C, 3C and 4C, respectively.

The peaks having a relative intensity (I/Io) of 3% or higher in the XRPD spectrum of the above crystalline form are shown in Table 7 below. When the peaks had I/Io ratios equal to or higher than 10%, they appeared at diffraction angles of 64°, 70°, 88°, 128°, 132°, 141°, 155°, 164°, 180°, 182°, 194°, 205°, 210°, 219°, 230°, 232°, 245°, 253°, 258°, 261°, 265°, 279°, 285°, 301°, 305°, and 310° (20 i 0.2°).

[Table 7] wo 2017/116192 20 (10.2) I 20 ($0.2) . I/lo (“/o) l/lo(%) 7 0 12.6 89.0 23.2 3.8 18.2 00 co ..5: o 13.8 24.5 3.6 22.2 6.7 g 22.4 26.1 3.4 13.7 j 2. . .5 4.3 22.0 35.5 2.5 6.4 .......... .......... 2'0 4.2. ,,,,, 74.6 35],,,,,,,,,,,, 2‘5 ”0,1 21.9 4.0 ] 13.5 37.4 2.4 8.8 22.1 4.0 i 9.4 39.8 2.3 5.2 : diffraction angle, (1: ce between (3051.211 faces, U10 (“/a): relative ily (‘1 indicates die intensity of each peak: 1., indicates the intensity of the highest peak.) In applying the ions of measurement as sed herein the above lline form showed an endothermic peak which has a starting point at about 50°C and its lowest point at about 73°C, an endothermic peak at about 189°C, and an endothermic peak at about 222°C, in a DSC (10°C /min). In the DSC, the endothermic peaks at about 73°C and 189°C indicate the dehydration point of the crystalline form of the di— hydrochloride trihydrate, and the endothermic peak at about 222°C indicates a melting point.

The above crystalline form showed a water content of about 8.9% (theoretical water content value: 8.8%) in a Karl Fischer titrator and a melting point from about 210°C to about 215°C.

In the DVS for the above crystalline form, the level of water absorption in the region with a relative humidity of 10% to 30% was very high, however, that in the region with a relative humidity of 40% or higher was measured to be very weak. The above lline form was expected to maintain the crystalline form of the trihydrate due to absorption of water under a long—term storage condition (e. g., a temperature of 25°C and a relative humidity of 60%) and an rated condition (e.g. a temperature of 40°C and a relative humidity of 75%).

In the ssNMR spectroscopy for the above crystalline form, the observed peaks were collected in the Table 8 below (expressed in ppm i 0.2 ppm): [Table 8] 2016/015535 Chemical Shift al Shift Chemical Shift Peak # Peak # Peak # (ppm) (ppm) (911m) 1 43.8 8 119.2 15 147.6 2 46.7 9 120.6 16 149.5 3 49.9 10 130.1 17 150.4 4 53.8 11 131.5 18 153.1 110.0 12 132.7 19 157.2 6 111.9 13 140.4 20 165.6 7 117.7 14 144.2 21 166.7 Example 5: Preparation of a crystalline form of a monohydrochloride hydrate, preferably monohydrate (1HC1~1H20), of a compound of Formula 1 A compound of Formula 1 ed according to the method disclosed in WO 2011/162515 or a similar method thereof, as referenced herein, in an amount of 5.0 g (0.010 mol) was added into a mixed solvent ning 15 mL of water and 35 mL of ethanol. The reaction mixture was treated with 0.97 mL (0.011 mol) of HCl dropwise and stirred at room temperature for 12 hours, and the resulting precipitated solids were filtered. The filtered precipitate was washed with a mixed solvent containing 1.5 mL of water and 3.5 mL of ethanol and dried at 50°C to obtain 2.6 g of the title compound (yield: 48.0%).

Water content: 3.5% (theoretical value for a monohydrate: 3.33%) Ion chromatography: 6.7% (theoretical value for a drochloride: 7.0%) Analysis of characteristics The results of XRPD, DSC, DVS and ssNMR analyses of the crystalline form prepared in Example 5 are shown in FIGS. 1E, 2D, 3D and 4D, respectively.

The peaks having a relative intensity (I/Io) of 3% or higher in the XRPD um of the above crystalline form are shown in Table 9 below. When the peaks had I/Io ratios equal to or higher than 10%, they appeared at diffraction angles of 78°, 107°, 127°, 130°, 139°, 142°, 156°, 170°, 17.7°, 186°, 191°, 195°, 21.5°, 220°, 225°, 246°, 253°, 257°, 260°, 264°, 277°, 282°, 295°, and 34.80 (20 1 02°).

[Table 9] 2016/015535 29(482) a [n.(%g 20(i02) d UL(96) 7.8 11.3 90.3 25.7 3.5 100 .7 8.2 35.6 26.0 3.4 17.9 12.7 7.0 23.1 26.4 3.4 17.1 13.0 6.8 39.6 27.4 3.3 8.2 13.6 6.5 3.9 27.7 3.2 15.6 13.9 6.4 25.5 28.2 3.2 18.5 14.2 6.2 19.1 29.0 3.1 6.6 .1 5.9 4.2 29.5 3.0 11.3 .6 5.7 11.8 30.0 3.0 5.5 17.0 5.2 11.3 30.7 2.9 8.6 17.7 5.0 21.5 31.5 2.8 7.5 18.6 4.8 29.8 32.9 2.7 5.1 19.1 4.6 47.1 33.4 2.7 5.4 19.5 4.6 10.6 34.0 2.6 7.5 .0 4.4 9.5 34.8 2.6 10.1 21.5 4.1 20.6 35.5 2.5 9.0 22.0 4.0 35.6 36.9 2.4 3.5 22.5 4.0 68 37.6 2.4 6.1 23.4 3.8 8.1 37.9 2.4 7.3 24.6 3.6 32.7 38.7 2.3 5.5 .3 3.5 33 39.3 2.3 4.6 29: diffraction angle, d: distance between l faces, 1/[.;,(%): relative intensity (1 indicates the intensity of each peak; [cindicates the intensity of the highest peak.) In applying the conditions of measurement as disclosed herein the above crystalline form showed an endothermic peak which has a starting point at about 115°C and its lowest point at about 142°C, an exothermic peak at about 204°C, and an endothermic peak which has a starting point at about 210°C and its lowest point at about 251°C, in a DSC (10°C /min). In the DSC, the endothermic peak at about 142°C indicates the de— hydration point of the crystalline form of the monohydrochloride monohydrate, and the exothermic peak at about 204°C indicates the occurrence of a partial phase tion, and an endothermic peak at about 251°C indicates a melting point.

The above crystalline form showed a water content of about 3.5% etical water t value of a monohydrate: 3.33%) in a Karl Fischer titrator and a melting point from about 190°C to about 200°C.

In the DVS for the above crystalline form, the level of water absorption in the region with a relative humidity of 10% to 70% was very low, however, that in the region with a relative humidity of 70% or higher was measured to be about 7%. From these results, the above lline form was ed to be stable under a long—term storage condition (e. g., a temperature of 25°C and a relative humidity of 60%), and stable under an accelerated condition (e. g., a temperature of 40°C and a relative humidity of 75%) due to absorption of water.

In the ssNMR spectroscopy for the above crystalline form, the observed peaks were collected in Table 10 (expressed in ppm 1 0.2 ppm): [Table 10] Chemical Shift Chemical Shift al Shift Peak # Peak # Peak # (rpm) (ppm) (ppm) i 42.5 s 116.9 15 142.1 2 45.4 9 120.1 16 146.3 3 51.0 10 122.9 17 153.5 Example 6. ation of a crystalline form (Type A) of a monohydrochloride hydrate, preferably dihydrate (1HC1-2H20), of a compound of Formula 1 A dihydrochloride hydrate, preferably monohydrate, (Type A) of the compound of Formula 1 prepared in Example 1 in an amount of 30.0 g was added to 900 mL of water. The mixture was stirred at room temperature for 72 hours, and the resulting pre— cipitated solids were filtered. The filtered precipitate was washed with 60 mL of the same solvent and dried to obtain 20 g of the title compound (yield: 67.0%). [352 Water content: 6.8% (theoretical value for a dihydrate: 6.45%) [353 Ion chromatography: 6.9% (theoretical value for a monohydrochloride: 7.0%) [354 [355|_11_i|_11_1 Analysis of characteristics The results of XRPD, DSC, DVS and ssNMR analyses of the crystalline form prepared in Example 6 are shown in FIGS. 1F, 2E, 3E and 4E, respectively.

The peaks having a relative ity (I/Io) of 3% or higher in the XRPD spectrum of the above crystalline form are shown in Table 11 below. When the peaks had I/Io ratios equal to or higher than 10%, they appeared at ction angles of 68°, 75°, 151°, 170°, 18.1°, 200°, 212°, 227°, 230°, 251°, and 265° (20 i 0.2°).

[Table 11] 20 ($0.2) d I/IG {%) 20 (1:02) (1 I/l0 ("/u) 6.8 12.9 12.5 23.0 3.9 10 7.5 11.8 100 23.9 3.7 3.6 12.4 7.1 9.7 24.2 3.7 3.2 13.2 6.7 2.9 25.1 3.5 20.1 .1 5.9 54.1 25.4 3.5 6.1 17.0 5.2 10.4 26.5 3.4 14.3 18.1 4.9 12.5 27.3 3.3 2.9 .0 4.4 39.4 28.6 3.1 3.8 .7 4.3 6.6 29.6 3.0 5.0 21.2 4.2 16.1 30.5 2.9 5.6 21.9 4.1 5.6 31.3 2.9 6.1 22.7 3.9 12.4 33.7 2.7 2.5 : diffraction angle, d: distance between crystal faces. {/10 (%): relative intensity (1 indicates the intensity of each peak; 10 tes the ity of the highest peak.) In applying the conditions of measurement as disclosed herein the above crystalline form showed an ermic peak which has a starting point at about 62°C and its lowest point at about 90°C, and an endothermic peak which has a starting point at about 171°C and its lowest point at about 182°C, in a DSC (10°C /min). In the DSC, the endothermic peak at about 90°C indicates the dehydration point of the crystalline form of the monohydrochloride dihydrate, and an endothermic peak at about 182°C indicates a melting point.

The above crystalline form showed a water content of about 6.8% (theoretical water content value: 6.45%) in a Karl Fischer titrator and a melting point from about 190°C to about 200°C.

In the DVS for the above crystalline form, the level of water absorption in the region with a relative humidity of 10% to 90% was measured to be as low as about 2%. The above crystalline form was expected to be stable under a long-term storage condition ( e.g., a ature of 25°C and a relative humidity of 60%) and an accelerated condition (e.g., a temperature of 40°C and a relative humidity of 75%).

In the ssNMR spectroscopy for the above crystalline form, the observed peaks were collected in Table 12 below (expressed in ppm i 0.2 ppm): [Table 12] al Shift Chemical Shift Chemical Shift Peak# Peak # Pcak# (ppm) (ppm) (991") | 43.1 7 117.6 13 137.8 2 46.5 8 122.4 14 146.0 3 48.1 9 123.1 15 151.7 4 53.2 10 127.6 16 157.6 107.5 11 130.0 17 164.3 6 115.9 12 133.4 18 165.0 Example 7. ation of a crystalline form (Type B) of a monohydrochloride hydrate, preferably dihydrate (lHCl-2H20), of a compound of Formula 1 The ochloride hydrate, preferably monohydrate, of the compound of a 1 prepared in Example 1 in the amount of 15.0 g (0.026 mol) was added into a mixed solvent consisting of water (45 mL) and ethanol (105 mL). To the reaction mixture was dropwise added with an aqueous solution, in which 2.18 g (0.055 mol) of sodium hydroxide was dissolved in 2.18 g (0.055 mol) of water, stirred at room temperature for 30 minutes, and dropwise added with 2.75 mL (0.031 mol) hloric acid. The reaction mixture was stirred at room ature for 12 hours, and the resulting pre— cipitated solids were filtered. The filtered precipitate was washed with a mixed solvent consisting of water (4.5 mL) and ethanol (10.5 mL), and dried at 50°C to obtain 8.5 g of the title compound (yield: 60.0%). [367 Water t: 6.0% (theoretical value for a dihydrate: 6.45%) [368 Ion chromatography: 7.2% (theoretical value for a monohydrochloride: 7.0%) [369 Analysis of characteristics [370|_11_1|_11_1 The results of XRPD, DSC, DVS and ssNMR analyses of the crystalline form 2016/015535 prepared in Example 7 are shown in FIGS. 1G, 2F, 3F and 4F, respectively.

The peaks having a relative ity (I/Io) of 3% or higher in the XRPD spectrum of the above crystalline form are shown in Table 13 below. When the peaks had I/Io ratios equal to or higher than 10%, they appeared at diffraction angles of 8.7°, 11.6°, 131°, 133°, 144°, 153°, 175°, 18.1°, 186°, 194°, 201°, 208°, 219°, 231°, 242°, 26.1°, 266°, 272°, 280°, 305°, and 31.7° (20 i 0.2°).

[Table 13] 20 (40.2) d 1/1,(%) 20 (20.2) d 171002..) 7.9 11.3 3.3 24.6 3.6 9.7 8.7 10.2 100 25.3 3.5 6.0 .4 8.5 3.0 26.1 3.4 30.8 11.6 7.7 29.6 26.6 3.3 15.5 13.1 6.8 16.4 27.2 3.3 13.1 13.3 6.7 18 28.0 3.2 20.4 14.4 6.1 21.5 28.8 3.1 6.4 .0 5.9 4.8 29.9 3.0 4.8 .3 5.8 11.8 30.5 2.9 11.5 16.6 5.3 3.4 30.9 2.9 7.2 17.5 5.1 33.5 31.7 2.8 14.3 18.1 4.9 12.0 32.4 2.8 7.2 18.6 4.8 13.8 33.2 2.7 3.1 19.4 4.6 98.8 35.2 2.5 4.2 .1 4.4 13.6 35.6 2.5 5.3 .8 4.3 23.8 36.7 2.4 6.9 21.9 4.1 24.1 37.7 2.4 3.9 23.1 3.8 48.5 39.4 2.3 3.0 24.2 3.7 12.5 : diffraction angle, 6!: distance between crystal faces, {/10 (‘34:): relative intensity (1 indicates the ity of each peak; Io indicates the intensity of the highest peak.) In applying the conditions of measurement as sed herein the above crystalline form showed an endothermic peak which has a starting point at about 55°C and its lowest point at about 71°C, and an endothermic peak which has a starting point at about 215°C and its lowest point at about 222°C, in a DSC (10°C /min). In the DSC, the endothermic peak at about 71°C indicates the dehydration point of the crystalline form of the monohydrochloride dihydrate, and an endothermic peak at about 222°C indicates a melting point.

The above crystalline form showed a water content of about 6.0% (theoretical water content value: 6.45%) in a Karl Fischer titrator and a melting point from about 190°C to about 200°C.

In the DVS for the above crystalline form, the level of water absorption in the region with a relative humidity of 10% to 70% was very low, but the level of water absorption in the region with a relative humidity of 70% or higher was measured to be about 14%.

From these, the above lline form was ed to be stable under a long—term WO 16192 storage condition (e. g., a temperature of 25°C and a relative humidity of 60%) and an accelerated ion (e.g., a temperature of 40°C and a relative humidity of 75%).

In the ssNMR spectroscopy for the above lline form, the observed peaks were collected in Table 14 below (expressed in ppm i 0.2 ppm): [Table 14] Chemical Shift Chemical Shift. Chemical Shift Peak # Peak # Peak # (pm) (ppm) 1me} 1 41.3 8 117.2 15 132.9 2 42.6 9 119.0 16 139.0 3 48.8 10 [214 I7 1457 4 50.1 11 122.0 18 152.7 3 55.9 12 122.6 19 157.4 6 109.4 13 128.0 20 163.5 7 111.5 14 130.7 21 165.0 Comparative Example 1: Preparation of an amorphous form of a dihy- drochloride (2HCl) of a compound of Formula 1 A dihydrochloride hydrate, preferably monohydrate, (Type A) of the compound of Formula 1 (Example 1) in an amount of 10 g was added to 200 mL of methanol. The mixture was d at 40°C for 30 minutes, and the resulting insoluble solids were filtered. The filtered itate was distilled under reduced pressure to obtain 9.0 g of the title compound (yield: 90.0%).

Water content: 6.9% Ion chromatography: 12.1% (theoretical value of a dihydrochloride: 13.0%) Analysis of characteristics The results of XRPD, DSC, DVS and ssNMR analyses of the amorphous form prepared in Comparative Example 1 are shown in FIGS. 1H, 2G, 3G and 4G, re- spectively.

The amorphous form failed to show any particular diffraction pattern in an XRPD spectrum.

Additionally, the amorphous form failed to show any particular ermic/ exothermic curve in a DSC (10°C/min).

Additionally, the amorphous form showed a very high level of water tion in the region with a relative humidity of 10% to 90% in a DVS. From these results, the above ous form was expected to be unstable under a long—term storage condition (e. g., a temperature of 25°C and a relative humidity of 60%) and under an ac— celerated condition (e.g., a temperature of 40°C and a relative humidity of 75%) due to absorption of water, and in fact, was shown to have a hygroscopicity of 13% to 15% under conditions of a ature of 25°C and a relative humidity of 60%; and a tem— perature of 40°C and a relative humidity of 75%.

Additionally, the amorphous form showed a significant tion in its water content, as measured by a Karl Fischer titrator, showing a water content of 4% to 8% (theoretical water t value: 8.81%). The melting point was not particularly specified and the decomposition at about 250°C was observed.

In the ssNMR spectroscopy for the above amorphous form, the observed peaks were collected in the Table 15 below ssed in ppm i 0.2 ppm): [Table 15] Chemical Shift Chemical Shift Chemical Shift Peak # Peak # Peak# (am) (pm) (pm) 44.5 4 [W] 7 |5|.I 2 49.8 5 130.2 8 164.8 Test Example 1: Test of measurement of water solubility In order to measure water solubility, each sample of the polymorphs of the hy- drochloride salts of the compound of Formula 1, prepared in Examples 1 to 7, was prepared in nic water under the conditions described below. Each of the solutions was analyzed by high performance liquid chromatography (HPLC) according to the conditions for measurement of contents of the compound of Formula 1, and the amount dissolved based on the amount of the compound of Formula 1 was measured (LOD: >0.00l mg/mL), and the values were ated. The results are shown in Table 16 below.

Specifically, each sample of the polymorphs in an amount of 500 mg was added to 5 mL of water, blended at 20°C to 25°C using a ixer, and filtered with a GH Polypro membrane Acrodisc, PALL (pore size: 0.2 mm). The filtrate was diluted with a diluent used for HPLC in a 1:100 ratio to obtain the samples.

[Table 16] Formula 1 Polymorph (amorphous Example 1 Example 3 Example 5 Example 6 Example 4 free base) C‘m' ”1 ”mm“ 100 100 100 100 100 100 (mg/mL) Solubility (mgi'mL) 0.00m 50.39 7.76 10.66 0.42 0.57 pll ofsolution 7.3 1.7 2.| 1.7 4.7 4.9 As shown in Table 16 above, the solubility of the hydrochloride salt of the compound of Formula 1 was significantly higher than that of the compound (free base) of a 1. In ular, the solubility of the crystalline form of the dihydrochloride salt of the compound of Formula 1 was significantly higher than that of the — drochloride, and the crystalline form of Example 1 showed the highest water solubility among the lline polymorphs of the dihydrochloride salt.

Accordingly, considering the conditions such as elution, etc, the crystalline form of e 1 (the dihydrochloride hydrate, preferably monohydrate, (Type A) of the compound of a 1) is expected to be most advantageous from the aspect of a pharmaceutical composition.

Claims (31)

Claims
1. [Claim 1] A hydrochloride salt of the compound of Formula 1: [Formula 1] \ WQL
2. [Claim 2] A crystalline form of a hydrochloride salt of the nd of a 1 according to claim 1.
3. [Claim 3] The crystalline form of the hydrochloride salt according to claim 2, wherein the salt is a monohydrochloride.
4. [Claim 4] The crystalline form of the hydrochloride salt ing to claim 2, wherein the salt is a ochloride.
5. [Claim 5] The crystalline form of the hydrochloride salt according to any one of claims 2 to 4, wherein the salt is a hydrate.
6. [Claim 6] The crystalline form of the hydrochloride salt according to claim 5, wherein the salt is a monohydrate.
7. [Claim 7] The crystalline form of the hydrochloride salt ing to claim 5, wherein the salt is a rate.
8. [Claim 8] The crystalline form of the hydrochloride salt according to claim 5, wherein the salt is a dihydrate.
9. [Claim 9] The crystalline form of the hydrochloride salt of claim 2, wherein the crystalline form is a ochloride hydrate, preferably monohydrate (2HC1~1H20), of the compound of Formula 1 having an X-ray powder diffraction (XRPD) pattern comprising peaks at diffraction angle 26 values of 5.60: 0.20 and 27.3% 02" when irradiated with a Cu-Ka light source.
10. [Claim 10] The crystalline form of the hydrochloride salt of claim 9, wherein the crystalline form further comprises peaks at diffraction angle 26 value of 21. l°iO.2°when irradiated with a Cu—Kor light source.
11. [Claim 11] The crystalline form of the hydrochloride salt of claim 9, wherein the crystalline form further comprises peaks at diffraction angle 26 value of 11. 1% 0.20 when irradiated with a Cu—Ka light source.
12. [Claim 12] The crystalline form of the hydrochloride salt of claim 11, n the crystalline form further comprises peaks at diffraction angle 26 value of 14.004; 0.2°and 20.804; 0.2°when irradiated with a Cu-Ka light source.
13. [Claim 13] The crystalline form of the hydrochloride salt of claim 2, wherein the crystalline form is a dihydrochloride hydrate, preferably monohydrate (2HCl-1H20), of the compound of Formula 1 having a 13C solid state NMR spectrum sing peaks at the following 13C chemical shifts: 44.6 i 0.2 ppm and 56.6 i 0.2 ppm.
14. [Claim 14] The crystalline form of the hydrochloride salt of claim 2, wherein the crystalline form is a dihydrochloride hydrate, preferably monohydrate (2HCl-1H20), of the compound of Formula 1 having a 13C solid state NMR spectrum comprising peaks at the following 13C al shifts: 149.6 i 0.2 ppm, 152.6 i 0.2 ppm and 164.3 i 0.2 ppm.
15. [Claim 15] The crystalline form of the hydrochloride salt of claim 2, wherein the crystalline form is a dihydrochloride hydrate, preferably monohydrate 1H20), of the compound of Formula 1 having (a) an X—ray powder diffraction (XRPD) pattern comprising peaks at diffraction angle 20 values of 5.6°i 0.20 and 27.3% 0.20 when ir- radiated with a Cu—Ka light source; and (b) a 13C solid state NMR spectrum comprising peaks at the following 13 C chemical shifts: 44.6 i 0.2 ppm and 56.6 i 0.2 ppm.
16. [Claim 16] The crystalline form of the hloride salt of claim 2, wherein the crystalline form is a dihydrochloride hydrate, preferably monohydrate 1H20), of the compound of Formula 1 having (a) an X-ray powder diffraction (XRPD) pattern sing peaks at ction angle 20 values of 5.6°i 0.20 and 27.3% 0.20 when ir- radiated with a Cu—Kor light source; and (b) a 13C solid state NMR spectrum comprising peaks at the following 13 C chemical shifts: 149.6 i 0.2 ppm, 152.6 i 0.2 ppm and 164.3 i 0.2 ppm.
17. [Claim 17] The lline form of the hydrochloride salt of claim 2, wherein the crystalline form is a dihydrochloride hydrate, preferably monohydrate 1H20), of the compound of Formula 1 having an X—ray powder diffraction pattern comprising peaks at diffraction angle 20 values of 6.4°i 0.2°, 12.8°i 0.20, 20.801L 0.2° and 22.0°i 0.20 when irradiated with a Cu—Ka light source.
18. [Claim 18] The crystalline form of the hydrochloride salt of claim 17, wherein the crystalline form further comprises peaks at diffraction angle 20 value of 8.1°i 0.2°, 9.7°J_r 0.20, 16.0°i 0.20, 24.1°i 0.20, 26.3°i 0.20, and 27. 1°1— 0.2°when irradiated with a Cu-Kor light source.
19. [Claim 19] The crystalline form of the hydrochloride salt of claim 2, wherein the crystalline form is a dihydrochloride hydrate, preferably monohydrate (2HC1~1H20), of the compound of Formula 1 having a 13C solid state NMR spectrum comprising peaks at the following 13C chemical shifts: 43.4 i 0.2 ppm and 45.2 i 0.2 ppm.
20. [Claim 20] The crystalline form of the hloride salt of claim 2, n the lline form is a dihydrochloride hydrate, preferably monohydrate 1H20), of the compound of Formula 1 having a 13C solid state NMR spectrum comprising peaks at the following 13C chemical shifts: 117.0 i 0.2 ppm, 149.8 i 0.2 ppm and 165.2 i 0.2 ppm.
21. [Claim 21] The crystalline form of the hydrochloride salt of claim 2, wherein the crystalline form is a dihydrochloride e, preferably drate (2HCl-1HZO), of the compound of Formula 1 having (a) an X—ray powder diffraction (XRPD) pattern comprising peaks at diffraction angle 26 values of 6.4°i0.2°, 12.8Oi0.2°, 20.8°i—O.2° and 22.0"i0.2O when irradiated with a Cu—Ka light source; and (b) a 13C solid state NMR spectrum comprising peaks at the following 13 C chemical shifts: 43.4 i 0.2 ppm and 45.2 i 0.2 ppm.
22. [Claim 22] The crystalline form of the hydrochloride salt of claim 2, wherein the crystalline form is a dihydrochloride hydrate, preferably monohydrate 1HZO), of the compound of Formula 1 having (a) an X—ray powder diffraction (XRPD) pattern comprising peaks at diffraction angle 26 values of 6.4Oi0.2°, 12.8°i0.2°, 20.80102O and 22.0"i0.2O when irradiated with a Cu—Koz light ; and (b) a 13C solid state NMR spectrum comprising peaks at the following 13 C chemical shifts: 117.0 i 0.2 ppm, 149.8 i 0.2 ppm and 165.2 i 0.2 ppm.
23. [Claim 23] The lline form of the hydrochloride salt of claim 2, wherein the crystalline form is a dihydrochloride hydrate, preferably trihydrate 3H20), of the compound of a 1 having an X—ray powder diffraction pattern comprising peaks at diffraction angle 26 values of 4.6% 0.20, 8.6% 0.20, and 15.8°i 0.20 when irradiated with a Cu-Kor light source.
24. [Claim 24] The crystalline form of the hydrochloride salt of claim 23, wherein the crystalline form further comprises peaks at diffraction angle 26 values of 17.20;L 0.20, 19.704; 0.20, 25.104; 0.20, and 26.304; 0.20 when ir- radiated with a Cu—Kor light source.
25. [Claim 25] The crystalline form of the hydrochloride salt of claim 2, wherein the lline form is a dihydrochloride hydrate, preferably trihydrate (2HCl-3H20), of the nd of Formula 1 having a 13C solid state NMR spectrum comprising peaks at the following 13C chemical shifts: 45.0 i 0.2 ppm and 53.8 i 0.2 ppm.
26. [Claim 26] The crystalline form of the hydrochloride salt of claim 2, wherein the crystalline form is a dihydrochloride hydrate, preferably trihydrate 3H20), of the compound of Formula 1 having a 13C solid state NMR spectrum sing peaks at the following 13C chemical shifts: 117.6 i 0.2 ppm and 150.2 i 0.2 ppm.
27. [Claim 27] The crystalline form of the hydrochloride salt of claim 2, wherein the crystalline form is a dihydrochloride e, preferably trihydrate (2HCl-3H20), of the compound of Formula 1 having (a) an X—ray powder diffraction (XRPD) pattern comprising peaks at diffraction angle 26 values of 4.6% 0.20, 8.6% 02", and 15.8% 0.20 when ated with a Cu—Ka light source; and (b) a 13C solid state NMR spectrum comprising peaks at the following 13 C chemical shifts: 45.0 i 0.2 ppm and 53.8 i 0.2 ppm.
28. [Claim 28] The crystalline form of the hydrochloride salt of claim 2, wherein the crystalline form is a dihydrochloride hydrate, preferably trihydrate (2HCl-3HZO), of the compound of Formula 1 having (a) an X-ray powder diffraction (XRPD) pattern comprising peaks at diffraction angle 26 values of 4.6% 0.20, 8.6% 02", and 15.8% 0.20 when irradiated with a Cu—Ka light source; and (b) a 13C solid state NMR um comprising peaks at the following 13 C chemical shifts: 117.6 i 0.2 ppm and 150.2 i 0.2 ppm.
29. [Claim 29] The crystalline form of the hydrochloride salt of claim 2, wherein the crystalline form is a dihydrochloride hydrate, preferably trihydrate (2HCl-3HZO), of the compound of Formula 1 having an X—ray powder diffraction pattern comprising peaks at diffraction angle 26 values of 6.4% 02°, 7.0% 0.20, 12.8% 0.20 and 21.0% 0.20 when irradiated with a Cu—Ka light source.
30. [Claim 30] The crystalline form of the hydrochloride salt of claim 29, wherein the crystalline form further comprises peaks at diffraction angle 26 values of 15.5% 0.20, 18.2% 0.20 and 27.9% 0.20 when irradiated with a Cu- K or light .
31. [Claim 31] The crystalline form of the hydrochloride salt of claim 2, wherein the crystalline form is a dihydrochloride e, ably trihydrate (2HCl-3H20), of the compound of Formula 1 having a 13C solid state WO 16192