NZ729218B2 - Neonatal Fc Receptor Binding Dimer and Methods of Use - Google Patents

Neonatal Fc Receptor Binding Dimer and Methods of Use Download PDF

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Publication number
NZ729218B2
NZ729218B2 NZ729218A NZ72921815A NZ729218B2 NZ 729218 B2 NZ729218 B2 NZ 729218B2 NZ 729218 A NZ729218 A NZ 729218A NZ 72921815 A NZ72921815 A NZ 72921815A NZ 729218 B2 NZ729218 B2 NZ 729218B2
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seq
fcrn
group
sequence
fcrn binding
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NZ729218A
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NZ729218A (en
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Lars Abrahmsen
Caroline Ekblad
Fredrik Frejd
Torbjorn Graslund
Lindvi Gudmundsdotter
Elin Gunneriusson
Malin Lindborg
John Lofblom
Johan Seijsing
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Affibody Ab
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Priority claimed from PCT/EP2015/071339 external-priority patent/WO2016042083A1/en
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Publication of NZ729218B2 publication Critical patent/NZ729218B2/en

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Abstract

The present disclosure relates to dimers of engineered polypeptides having a binding affinity for the neonatal Fc receptor FcRn, and provides an FcRn binding dimer, comprising a first monomer unit, a second monomer unit and an amino acid linker, wherein said first and second monomer unitseach comprisesan FcRn binding motif. Said FcRn binding dimerbinds FcRn with higher capacity compared to said first monomer unitor second monomer unit alone.The present disclosure also relates to the use of said FcRn binding dimeras an agent for modifying pharmacokinetic and pharmacodynamic properties and as a therapeutic agent. sesan FcRn binding motif. Said FcRn binding dimerbinds FcRn with higher capacity compared to said first monomer unitor second monomer unit alone.The present disclosure also relates to the use of said FcRn binding dimeras an agent for modifying pharmacokinetic and pharmacodynamic properties and as a therapeutic agent.

Description

NEONATAL FC RECEPTOR BINDING DIMER AND METHODS OF USE Technical field of the invention The present disclosure relates to dimers of engineered polypeptides having a binding affinity for the neonatal Fc receptor (in the following referred to as FcRn). The present disclosure also relates to the use of such dimers as agents for modifying pharmacokinetic and codynamic ties of a biomolecule, e.g. a pharmaceutical, and as therapeutic agents.
Background The neonatal Fc receptor (FcRn) is a heterodimeric protein consisting of a transmembrane MHC class I-like heavy chain (FcRn α-chain) and the β2- microglobulin light chain, the latter also forming a part of MHC class I molecules (Simister and Mostov (1989) Nature 337:184-7; Burmeister et al. (1994) Nature 372:379-83).
FcRn is predominantly located in endosomes and is able to bind to serum n and immunoglobulin G (IgG) at pH ≤ 6.5 and release them at pH ≥ 7.0 wed in Roopenian (2007) Nat Rev Immunol 7:715-25).
FcRn carries out several distinct tasks in mammals (Roopenian, supra). FcRn is involved in recycling of endocytosed IgG and serum albumin, thus avoiding their degradation in the lysosome, giving them longer half-life and higher availability in the blood than other serum proteins. When IgG, serum albumin and other serum proteins are passively pinocytosed by cells in contact with blood, the pH becomes gradually lower in the formed endosomes, which permits the binding of IgG and serum albumin to FcRn.
The receptor is then, together with its bound ligand, orted via ing endosomes back to the plasma ne. After returning to the plasma membrane, the pH increases to above 7, at which point the bound ligand is released.
FcRn is also ized for its ability to transport IgG over barriers such as the placenta, the upper airway lium, the blood-brain barrier and the proximal small intestine.
W0 2016;042083 2015/071339 In mammals, the properties of FcRn are used to transcytose lgG from a mother to a fetus via the placenta, and to transcytose lgG from a mother’s milk to the blood stream of an infant in the proximal small intestine.
The expression pattern of FcRn differs between species. However, FcRn is widely expressed by cells in the blood brain barrier, upper airway epithelium, kidneys and vascular endothelia, and by antigen ting cells as well as by other cells of hematopoietic origin in most species (Roopenian (2007), supra).
Antibodies and peptides with affinity towards FcRn (Liu et al. (2007) J Immunol 179:2999-3011, Mezo et al. (2008) Proc Natl Acad Sci U S A 105:2337—42) and B2-microglobulin (Getman and Balthasar (2005) J Pharm Sci 94:718—29) have been developed with a view to t the binding between endogenous lgG and FcRn. r approach has been to mutate the Fc region of the lgG to get a higher affinity for FcRn (Petkova et al. (2006) Int Immunol 9-69, o etal. (2005) Nat Biotechnol 23:1283-8).
Fusion to the Fc domain or to albumin is a widely used strategy to increase the in vivo half-life of proteins. r, the large size of such fusion proteins adversely affects tissue penetration and reduces the specificity to the fusion partner s et al. (2011) J Interferon Cytokine Res 32:178—184). On the other hand, mutations have been made in the Fc fragment of antibodies administered to non human primates to prolong half-life (Hinton etal. (2004) J Biol Chem 279:6213—6). However, this approach is only limited in use to therapeutic antibodies, and cannot be extrapolated to other therapeutic proteins unless the proteins in on are fused to F0 fragments, which also results in large size molecules. A number of chemical and recombinant methods have been devised to improve protein half-life, such as PEGylation and genetic fusions of the protein to the Fc domain of lgG or albumin (reviewed in Schellenberger et al. (2009) Nat Biotechnol 21:1186-1190).
PEGylation of ns has been reported to decrease their potency and contribute to their immunoreactivity.
Fc—fusion proteins have also been used for oral and pulmonary ry mediated by the FcRn (Low et al., (2005) Human reproduction Jul;20(7):1805- 13), however similar problems relating to tissue ation and reduced specificity remain, due to the size of the fusion molecules.
Hence, there is large need in the field for the ued provision of molecules with high affinity for FcRn. In particular, small binding molecules W0 2016;042083 are needed that, when present as a fusion r, do not adversely affect the properties of the molecules they are fused to and do not contribute to immunoreactivity.
Summary of the invention The present disclosure is based on the unexpected realization that FcRn binding ptides in dimeric form exhibit significantly improved FcRn binding properties as compared to corresponding FcRn binding polypeptides in monomeric form. The present inventors have found that the improvement in the binding properties of said FcRn binding polypeptides in dimeric form is greater than anticipated by the mere fusion of two FcRn binding polypeptides.
Thus, it is an object of the present sure to provide new FcRn binding agents.
It is also an object of the present disclosure to provide such agents for use in modifying pharmacokinetic and/or pharmacodynamic properties of a biomolecule, eg. a pharmaceutical.
It is also an object of the present disclosure to provide such agents for use as therapeutic agents in their own right, alone or as combination treatment.
It is an object of the t disclosure to provide a molecule allowing for efficient targeting of FcRn, while alleviating the above-mentioned and other drawbacks of t therapies.
These and other s which are evident to the skilled person from the present disclosure are met by different aspects of the ion as claimed in the appended claims and as generally disclosed herein.
Thus, in the first aspect of the disclosure, there is ed a neonatal Fc receptor (FcRn) binding ptide in dimeric form, i.e. an “FcRn g dimer”, comprising a first monomer unit, a second monomer unit and an amino acid linker, wherein said first and second monomer unit each comprises an FcRn binding motif (BM), which motif consists of the amino acid sequence EX2 X3 X4 AXe X7 EIR WLPNLX15 X17 X18 QR X21 AF|X25 X25LX28 X29 wherein, independently from each other, W0 42083 X2 is selected from A, D, E, F, H, I, K, L, N, Q, R, S, T, V, W and Y; X3 is selected from A, D, E, F, G, H, I, K, L, M, N, Q, R, S, T, V, W and X4 is selected from A, D, E, F, G, H, l, K, L, N, Q, R, S, T, V, W and Y; X5 is selected from A, D, E, F, G, H, l, K, L, N, Q, R, S, T, V, W and Y; X7 is selected from A, F, H, l, K, L, N, Q, R, S, T, V, W and Y; X16 is selected from N and T; X17 is selected from F, W and Y; X18 is ed from A, D, E and N; X21 is selected from A, S, V and W; X25 is selected from A, D, E, F, G, H, l, K, L, N, Q, R, S, T, V, W and Y; X26 is selected from K and S; X28 is selected from A, D, E, F, H, l, K, L, N, Q, R, S, T, V, W and Y; X29 is selected from D and R, and n said FcRn binding dimer binds FcRn with a higher binding ty compared to said first monomer unit or said second monomer unit alone.
The above definition of a class of sequence related, FcRn binding motifs is based on a tical analysis of a number of random polypeptide monomer variants of a parent scaffold, that were selected for their interaction with FcRn in several different selection experiments. The identified FcRn binding motif, or “Bll/f’, ponds to the target binding region of the parent scaffold, which region constitutes two alpha helices within a three-helical bundle protein domain. In the parent scaffold, the varied amino acid residues of the two BM helices constitute a binding surface for interaction with the constant Fc part of antibodies. In the present disclosure, the random variation of binding surface residues and subsequent selection of variants have replaced the Fc interaction capacity with a capacity for ction with FcRn.
In one embodiment, the FcRn binding motif of at least one of said first and second monomer units consists of the amino acid sequence EX2 X3 X4 AXB X7 EIR WLPNLTX17 X18 QR X21 AF|X25 KLX28 D W0 42083 wherein, independently from each other, X2 is selected from A, D, E, F, H, I, K, L, N, Q, R, S, T, V, W and Y; X3 is selected from A, D, E, F, G, H, l, K, L, M, N, Q, R, S, T, V, W and Y; X4 is selected from A, D, E, F, G, H, l, K, L, N, Q, R, S, T, V, W and Y; X5 is selected from A, D, E, F, G, H, l, K, L, N, Q, R, S, T, V, W and Y; X7 is selected from A, F, H, l, K, L, N, Q, R, S, T, V, W and Y; X17 is selected from F, W and Y; X13 is selected from A, D, E and N; X21 is ed from A, S, V and W; X25 is selected from A, D, E, F, G, H, l, K, L, N, Q, R, S, T, V, W and Y; X23 is selected from A, D, E, F, H, l, K, L, N, Q, R, S, T, V, W and Y.
In one embodiment of the first aspect of the disclosure, said binding motif of at least one of said first and second monomer units ts of the amino acid sequence EX2 X3 X4 AXe X7 EIR WLPNLX16X17 X18 QR X21 AF|X25 X26LX28 X29 wherein, independently from each other, X2 is selected from A, D, E, F, H, l, K, L, N, Q, R, S, T, V, W and Y; X3 is selected from A, D, E, F, G, H, l, K, L, M, N, Q, R, S, T, V, W and X4 is selected from A, D, E, F, G, H, l, K, L, N, Q, R, S, T, V, W and Y; X3 is selected from A, E, F, G, H, l, K, Q, R, S and V; X7 is selected from A, F, H, K, N, Q, R, S and V; X13 is selected from N and T; X17 is selected from F, W and Y; X13 is selected from A, D, E and N; X21 is selected from A, S, V and W; X25 is selected from D, E, G, H, l, K, L, N, Q, R, S, T, V, W and Y; X26 is selected from K and S; X28 is selected from A, D, E, F, H, l, K, L, N, Q, R, S, T, V, W and Y; W0 2016;042083 X29 is selected from D and R.
In another embodiment of the first aspect, said binding motif of at least one of said first and second monomer units consists of such an amino acid sequence wherein, independently from each other, X2 is selected from A, D, E, F, H, l, K, L, N, Q, R, S, T, V, W and Y; X3 is selected from A, D, E, F, H, I, K, L, M, N, Q, R, S, T, V, W and Y; X4 is selected from A, D, E, F, H, l, K, L, N, Q, R, S, T, V, W and Y; X5 is selected from A, E, F, G, H, l, K, Q, R and 8; X7 is selected from A, F, H, K, N, Q, R, S and V; X16 is selected from N and T; X17 is selected from F and Y; X18 iS D; X21 iS Vi X25 is ed from D, E, H, l, K, L, N, Q, R, S, T, V, W and Y; X2; is selected from K and S; X28 is selected from A, D, E, F, H, l, K, L, N, Q, R, S, T, V and W and.
X29 is selected from D and R.
In r embodiment of the first aspect, said BM in at least one of said first and second monomer units consists of an amino acid sequence ed from i) EX2 X3 X4 AXB HEIR WLPNLTX17 X18 QR X21 AFlX25 KLX23 D wherein, independently from each other, X2 is selected from A, D, E, F, H, l, K, L, N, Q, R, S, T, V, W and Y; X3 is selected from A, D, E, G, H, K, L, M, N, Q, R, S, T, V and Y; X4 is selected from A, D, E, F, G, l, K, L, N, Q, R, S, T, V and Y; X6 is selected from A, G, K, R, S and V; X17 is selected from F, W and Y; X18 is selected from A, D, E and N; X21 is selected from A, S, V and W; X25 is selected from D, G, H, K, L, N, R, V and W; X28 is selected from A, D, E, H, K, L, N, Q, R, S, T, W and Y; W0 2016;042083 ii) an amino acid sequence which has at least 96 % identity to said sequence.
In yet another embodiment of said aspect, said BM in sequence i) consists of an amino acid sequence selected from EX2 X3 X4 AX6 HEIR WLPNLTX17 X18 QR X21 AF|X25 KLX28 D wherein, independently from each other, X2 is selected from A, D, E, F, N, Q, R, S and W; X3 is ed from D, E, G, H, K, M, N, Q, S and T; X4 is selected from A, D, E, G, N, Q, R, S, T, V and Y; X5 is selected from A, G, S and V; X17 is selected from F, W and Y; X18 is selected from A, D, E and N; X21 is selected from A, S, V and W; X25 is selected from D, G, H, K, L, N, R and V; and X28 is selected from A, E, H, L, N, Q, R, S, T, W and Y.
As the skilled person will realize, the function of any polypeptide, including the FcRn binding capacity of the dimer of the present disclosure, is dependent on the tertiary structure of the ptide. It is ore possible to make minor changes to the sequence of amino acids in a polypeptide without affecting the function thereof. Thus, the disclosure encompasses variants of the FcRn g dimer, for example variants wherein at least one of said first and second monomeric units is modified but the FcRn binding characteristics retained.
Therefore, as described above, also encompassed by the present disclosure is an FcRn binding dimer, n at least one of said first and second monomer units comprises an FcRn g motif (BM) comprising an amino acid sequence with 96 % or greater ty to a polypeptide as defined in i).
W0 2016;042083 In some embodiments, such changes may be made in all positions of the ces of the BM as disclosed herein. In other embodiments, such changes may be made only in the non-variable positions, also denoted as scaffold amino acid residues. In such cases, changes are not allowed in the variable positions, i.e. positions denoted with an “X” in sequence i). For example, it is possible that an amino acid residue belonging to a certain onal grouping of amino acid residues (e.g. hydrophobic, hydrophilic, polar etc) could be exchanged for r amino acid residue from the same functional group.
The term ”% identity”, as used throughout the specification, may for example be calculated as follows. The query sequence is aligned to the target sequence using the L W algorithm (Thompson et al. (1994) Nucleic Acids ch 22:4673-4680). A comparison is made over the window corresponding to the shortest of the aligned sequences. The shortest of the aligned sequences may in some ces be the target sequence. In other instances, the query ce may constitute the shortest of the aligned sequences. The amino acid residues at each position are compared, and the percentage of positions in the query ce that have identical correspondences in the target sequence is reported as % identity.
As used herein “Xn” and “Xm” are used to indicate amino acids in positions n and m in the sequence of the BM as d above, wherein n and m are integers which indicate the position of an amino acid within said sequence as counted from the N-terminal end of said sequence. For example, X3 and X7 indicate the amino acid in position three and seven, respectively, from the N-terminal end of said BM. ln embodiments according to the first aspect, there is provided an FcRn binding dimer, in which at least one of said first and second monomer units comprises an FcRn binding motif, wherein Xn is independently selected from a group of possible residues ing to Table 1. The skilled person will appreciate that Xn may be selected from any one of the listed groups of possible residues and that this ion is ndent from the selection of amino acids in Xm, wherein n¢m. Thus, any of the listed possible residues in position Xn in Table 1 may be independently combined with any of the herein disclosed possible residues in any other variable position in Table 1.
The skilled person will appreciate that Table 1 is to be read as follows: In one embodiment according to the first aspect, there is provided an FcRn WO 2016042083 binding dimer, wherein said first monomer unit and said second monomer unit each comprise an FcRn binding motif (BM) and wherein amino acid residue “X,” in the BM of at least one of said first monomer unit and said second r unit is ed from “Possible residues”. The skilled person will appreciate that the amino acid residue “X,” in BM of the first monomer unit is selected independently of the amino acid residue “Xn” in BM of the second monomer unit. Thus, Table 1 discloses several specific and individualized variants of the first r unit and the second monomer unit of the present disclosure. For example, in one ment, there is provided an FcRn binding dimer, comprising at least one first or second monomer unit, wherein X2 in BM is selected from A, l, L, N, Q, S, T, V and W, and in another embodiment, there is provided provided an FcRn binding dimer, comprising at least one first or second monomer unit, wherein X2 in BM is selected from A, l, L and Q. For avoidance of doubt, said first and second monomer units may be freely combined in other ments. For example, in one such ment, X3 is selected from A, D, E, G, H, K, L, M, N, Q, R, S and T, while X7 is selected from A and H, and X25 is selected from H, L, R, V and W.
Table 1: Possible residues in variable positions of the FcRn binding motif of the present disclosure.
Xn Possible residues Xn POSSIble reSIdues X2 A, D, E, F, I, L, N, Q, R, S, T, R, S, T, V, Y V, W, Y X3 A, D, E, H, K, L, M, N, Q, R, X2 A, D, F, I, L, N, Q, R, S, T, V, S, T, V, Y W, Y X3 A, D, E, G, H, K, L, M, N, Q, X2 A, D, F, I, L, N, Q, R, S, V, W R, S, T X2 A, I, L, N, Q, R, S, T, V, W, Y X3 A, D, E, G, H, K, M, N, Q, S, X2 A, I, L, N, Q, S, T, V, W T X2 A, I, L, N, Q, V, W X3 A, D, E, G, H, M, N, Q, S, T X2 A, I, L, Q, V, W X3 A, D, E, K, N, Q, S, T X2 A, I, L, Q, W X3 A, D, E, K, Q, T X2 A, I, L, Q X3 A, D, E, Q, T X2 I, L, Q X3 D, E, T X2 I, Q X3 D, Q, T X2 A, W X3 D, E X2 A X3 D, Q X2 W X3 D, T X2 I X3 Q, T X2 Q X3 D X3 A, D, E, G, H, K, L, M, N, Q, X3 E WO 20161042083 2015/071339 hhkh PTQASAAIAAAAAAAAAAAAADEQAAAAAAAAAAGAAHTNm mm mm VQQQQQQQQQDEQ EMEEIve}fiJ K L:N, are%ma mYM Q, R , QD E MM NAQK a R: Qu, v, N, AU; T, L R, S, W Q m, T., - XXXXXXXXX nnnmmmmmm Mhnhhhhhhhhhflhhhkk&k&&&&&&&M&kfifi EELEKEKKK twmxsxbKKQfi MQS K, N S I L Q R S T: X PEFAADMVQMQWEQHHHHHHHKSAIAWAAAAAQARDDR EWE HNQ % H, K L N Q R M A XXXXXXXXXXXXgamgggggg%%m HQLRRLRV LHRMVR N,K, QLW RN IR MM WWV,W QWQNQQQQRRENE H, K L M Q R S QQQQQQQQG KKKKKKKV bfifiswsRfififlfiN V, X% K, L M Q R S I XXXXXXXXXXXmgmwmmmmmmm EKKR L, L, Y? W IR W& N. RNR SQW HH KR In one embodiment, there is provided an FcRn binding dimer, wherein X6X7 is selected from AH and GH in at least one of said first and second monomer units. In one embodiment, X5X7 is AH. In one embodiment, X6X7 is W0 2016;042083 GH. In one embodiment, X17X18 is selected from FD and YD in at least one of said first and second monomer units. In one embodiment, X17X18 is FD.
In a more specific embodiment defining a sub-class of FcRn binding dimers, the sequence of the BM of at least one of said first and second r units fulfills at least three of the six conditions I-Vl: I. X5 is selected from A, G, K and S, such as in particular A; ll. X7 is H; III. X17 is selected from F and Y, such as in particular F; IV. X18 IS D; V. X21 is selected from V and W, such as in particular V; VI. X25 is selected from H and R, such as in particular H.
In some examples of an FcRn binding dimer according to the first , said sequence fulfills at least four of the six conditions l-Vl. More specifically, the sequence may fulfill at least five of the six conditions l-VI, such as all of the six conditions l-Vl.
In one embodiment, the BM sequences of said first and second monomer units are identical. In another embodiment, the BM sequences of said first and second monomer units are different.
As bed in detail in the experimental section to follow, the selection of FcRn binding polypeptide r units has led to the identification of a number of individual FcRn binding motif (BM) ces.
These sequences constitute individual ts useful as first and second monomer units as disclosed herein. The sequences of dual FcRn binding motifs (BMs) are presented in Figure 1 and correspond to the sequence from position 8 to position 36 in a sequence selected from the group consisting of SEQ ID NO:1-353. Hence, in one embodiment of the FcRn binding dimer according to the first aspect, at least one of said first and second monomer units comprises a BM ponding to the sequence from position 8 to position 36 in a sequence selected from the group consisting of SEQ ID NO:1-353, such as selected from the group consisting of SEQ ID NO:17-352. In one embodiment, said BM ce corresponds to the sequence from position 8 to position 36 in a sequence selected from the group consisting of SEQ ID 5, SEQ ID NO:17-140 and SEQ ID , such as the group consisting of SEQ ID NO:17-140. In one embodiment, said sequence corresponds to the sequence from position 8 to position 36 in a sequence ed from the group consisting of SEQ ID NO:1-2 and SEQ ID NO:17-140. In one embodiment, said sequence corresponds to the sequence from position 8 to position 36 in a sequence selected from the group consisting of SEQ ID NO:1-2, SEQ ID NO:17-92, SEQ ID NO:94-103, SEQ ID -125 and SEQ ID N02127-‘I40, such as the group consisting of SEQ ID NO:17-92, SEQ ID NO:94-103, SEQ ID NO:105-125 and SEQ ID NO:127-140. In one embodiment, said sequence corresponds to the sequence from position 8 to position 36 in a sequence selected from the group consisting of SEQ ID NO:1-8, SEQ ID NO:13, SEQ ID NO:19-20, SEQ ID NO:23, SEQ ID NO:28, SEQ ID NO:41, SEQ ID NO:44, SEQ ID NO:65, SEQ ID NO:70, SEQ ID NO:73, SEQ ID NO:75-77 and SEQ ID NO:353, such as the group consisting of SEQ ID NO:19-20, SEQ ID NO:23, SEQ ID NO:28, SEQ ID NO:41, SEQ ID NO:44, SEQ ID NO:65, SEQ ID NO:70, SEQ ID NO:73 and SEQ ID NO:75-77. In another embodiment, said sequence corresponds to the ce from position 8 to on 36 in a sequence selected from the group consisting of SEQ ID NO:1, SEQ ID NO:23, SEQ ID NO:28, SEQ ID NO:41, SEQ ID NO:44, SEQ ID NO:65, SEQ ID NO:73 and SEQ ID NO:75-77, such as the group consisting of SEQ ID NO:23, SEQ ID NO:28, SEQ ID NO:41, SEQ ID NO:44, SEQ ID NO:65, SEQ ID NO:73 and SEQ ID NO:75-77. In another embodiment, said sequence corresponds to the sequence from position 8 to position 36 in a sequence selected from the group consisting of SEQ ID NO:1, SEQ ID NO:20, SEQ ID NO:23, SEQ ID NO:28, SEQ ID NO:41, SEQ ID NO:44, SEQ ID NO:65, SEQ ID NO:73 and SEQ ID NO:75-77, such as the group consisting ofSEQ ID NO:20, SEQ ID NO:23, SEQ ID NO:28, SEQ ID NO:41, SEQ ID NO:44, SEQ ID NO:65, SEQ ID NO:73 and SEQ ID NO:75-77. In yet r embodiment, said ce corresponds to the sequence from position 8 to position 36 in a sequence ed from the group consisting of SEQ ID NO:1, SEQ ID NO:23, SEQ ID NO:44, SEQ ID NO:65, SEQ ID NO:75 and SEQ ID NO:77, such as the group consisting of SEQ ID NO:1, SEQ ID NO:23, SEQ ID NO:44, SEQ ID NO:65 and SEQ ID NO:75. In yet another embodiment, said sequence corresponds to the sequence from position 8 to position 36 in a sequence selected from the group consisting of SEQ ID N021, SEQ ID NO:20, SEQ ID NO:23, SEQ ID NO:41, SEQ ID NO:44, SEQ ID NO:65, SEQ WO 42083 ID NO:75 and SEQ ID NO:77, such as the group consisting of SEQ ID NO:1, SEQ ID NO:20, SEQ ID NO:23, SEQ ID NO:41, SEQ ID NO:44, SEQ ID NO:65 and SEQ ID NO:75. In yet another embodiment, said sequence corresponds to the sequence from on 8 to position 36 in a sequence selected from the group consisting of SEQ ID NO:23, SEQ ID NO:44, SEQ ID NO:65, SEQ ID NO:75 and SEQ ID NO:77, such as the group consisting of SEQ ID NO:23, SEQ ID NO:44, SEQ ID NO:65 and SEQ ID NO:75. In yet another embodiment, said sequence corresponds to the sequence from on 8 to position 36 in a sequence selected from the group consisting of SEQ ID NO:20, SEQ ID NO:23, SEQ ID NO:41, SEQ ID NO:44, SEQ ID NO:65, SEQ ID NO:75 and SEQ ID NO:77, such as the group consisting of SEQ ID NO:20, SEQ ID NO:23, SEQ ID NO:41, SEQ ID NO:44, SEQ ID NO:65 and SEQ ID NO:75, such as the group consisting of SEQ ID NO:20, SEQ ID NO:23, SEQ ID NO:41, SEQ ID NO:44 and SEQ ID NO:75. In one embodiment, said sequence corresponds to the sequence from position 8 to position 36 in a sequence selected from the group consisting of SEQ ID NO:1, SEQ ID N023 and SEQ ID NO:75, such as the group consisting of SEQ ID N023 and SEQ ID NO:75. In one embodiment, said sequence corresponds to the sequence from position 8 to position 36 in a sequence selected from the group consisting of SEQ ID NO:20, SEQ ID NO:41 and SEQ ID NO:44, such as the group consisting of SEQ ID N020 and SEQ ID NO:41; the group consisting of SEQ ID N020 and SEQ ID NO:44; or the group consisting of SEQ ID NO:41 and SEQ ID NO:44. In one embodiment, said sequence corresponds to the sequence from position 8 to position 36 in a sequence selected from the group consisting of SEQ ID NO:1, SEQ ID N023 and SEQ ID NO:44, such as the group consisting of SEQ ID N023 and SEQ ID NO:44. In one embodiment, said sequence corresponds to the sequence from on 8 to on 36 in SEQ ID NO:1 or SEQ ID NO:23 or SEQ ID NO:44. In one ment, said sequence corresponds to the sequence from position 8 to position 36 in SEQ ID NO:20 or SEQ ID NO:41 or SEQ ID NO:44 In one embodiment of the FcRn binding dimer as disclosed herein, both said first and second monomer units comprise a BM ponding to the sequence from position 8 to position 36 in a ce selected from one of the groups defined above. In one embodiment, said group consists of SEQ ID NO:1, SEQ ID NO:20, SEQ ID NO:23, SEQ ID NO:41, SEQ ID NO:44, SEQ ID NO:65 and SEQ ID NO:75. In one embodiment, said group consists WO 42083 of SEQ ID NO:20, SEQ ID NO:23, SEQ ID NO:41, SEQ ID NO:44 and SEQ ID NO:75. In one embodiment, said group consists of SEQ ID N021, SEQ ID NO:23, SEQ ID NO:44, SEQ ID NO:65 and SEQ ID NO:75. In another embodiment, said group consists of SEQ ID NO:1, SEQ ID N023 and SEQ ID NO:44. In yet another embodiment, said group consists of SEQ ID NO:20, SEQ ID NO:41 and SEQ ID NO:44. In one particular embodiment, both said first and second monomer units comprise a BM corresponding to the sequence from position 8 to position 36 in SEQ ID NO:1. In one embodiment, said BM corresponds to the sequence from position 8 to position 36 in SEQ ID NO:20. In one embodiment, said BM corresponds to the ce from position 8 to position 36 in SEQ ID NO:23. In one embodiment, said BM corresponds to the sequence from position 8 to position 36 in SEQ ID NO:41.
In one embodiment, said BM corresponds to the sequence from position 8 to on 36 in SEQ ID NO:44.
In some embodiments of the t disclosure, the BM as defined above “forms part of’ a helix bundle protein domain. This is understood to mean that the ce of the BM is “inserted” into or “grafted” onto the sequence of the original three-helix bundle domain, such that the BM es a similar structural motif in the original domain. For example, without wishing to be bound by theory, the BM is thought to constitute two of the three helices of a three-helix bundle, and can therefore replace such a two-helix motif within any three-helix bundle. As the d person will realize, the replacement of two helices of the three-helix bundle domain by the two BM helices has to be performed so as not to affect the basic structure of the polypeptide. That is, the l folding of the Ca backbone of the polypeptide according to this embodiment of the invention is substantially the same as that of the three-helix bundle protein domain of which it forms a part, e.g. having the same elements of secondary structure in the same order etc.
Thus, a BM according to the disclosure “forms part” of a three-helix bundle domain if the polypeptide according to this embodiment of the aspect has the same fold as the original domain, implying that the basic structural properties are shared, those properties e.g. resulting in similar CD spectra. The skilled person is aware of other parameters that are relevant.
In particular embodiments, the FcRn binding motif (BM) in at least one of said first and second monomers thus forms part of a helix bundle protein domain. For example, the BM may essentially constitute two alpha W0 2016;042083 helices with an interconnecting loop, within said helix bundle protein domain. In particular embodiments, said three-helix bundle protein domain is selected from domains of bacterial receptor proteins. Non-limiting examples of such domains are the five different helical domains of Protein A from lococcus aureus, such as domain B, and tives f. In some embodiments, the helical bundle protein domain is a variant of protein Z, which is derived from domain B of staphylococcal Protein A.
In embodiments where the FcRn binding motif as disclosed herein forms part of a three-helix bundle protein domain, at least one of said first and second monomer units of the FcRn binding dimer may comprise a binding module (BMod), which module consists of an amino acid ce selected from: iii) K-[BM]—DPSQS XaXbLLXC EAKKL XdXeXfQ; wherein [BM] is an FcRn binding motif as defined herein, provided that X29 is D; Xa is selected from A and S; Xb is selected from N and E; X0 is selected from A, S and C; Xd is selected from E, N and S; Xe is selected from D, E and S; Xf is selected from A and S; and iv) an amino acid sequence which has at least 93 % identity to a sequence defined by iii).
In embodiments where the FcRn binding motif as disclosed herein forms part of a three-helix bundle protein domain, at least one of said first and second monomer units of the FcRn binding dimer may comprise a binding module (BMod), which module consists of an amino acid ce selected from: v) K-[BM]—QPEQS XaXbLLXc EAKKL XdXeXfQ; W0 2016;042083 2015/071339 wherein [BM] is an FcRn binding motif as defined herein, provided that X29 is R; Xa is selected from A and S; Xb is selected from N and E; XC is selected from A, S and C; Xd is selected from E, N and S; Xe is selected from D, E and S; Xf is selected from A and S; vi) an amino acid sequence which has at least 93 % identity to a sequence defined by v).
As sed above, polypeptides sing minor s as compared to the above amino acid sequences which do not largely affect the tertiary structure and the function thereof are also within the scope of the present disclosure. Thus, in some embodiments, sequence iv) or sequence vi) has at least 95 %, for example at least 97 % identity to a sequence defined by iii) and v), respectively.
In one embodiment, there is provided an FcRn binding dimer, wherein at least one of said first and second monomer units comprises sequence iii) or v) wherein Xa is A. In an alternative embodiment, Xa in sequence iii) or v) is S. In one embodiment, X2] in sequence iii) or v) is A. In one embodiment, X2] in sequence iii) or v) is S.
In one embodiment, there is provided an FcRn g dimer, wherein at least one of said first and second monomer units ses sequence iii) or v) wherein Xb is N. In one embodiment, Xb in sequence iii) or v) is E.
In one embodiment, there is provided an FcRn binding dimer, wherein at least one of said first and second monomer units ses sequence iii) or v) wherein XC is A. In one embodiment, XC in sequence iii) or v) is S. In one embodiment, XC in sequence iii) or v) is C.
In one embodiment, there is provided an FcRn binding dimer, wherein at least one of said first and second monomer units comprises sequence iii) or v) wherein Xd is E. In one embodiment, Xd in sequence iii) or v) is N. In one embodiment, Xd in sequence iii) or v) is S.
W0 2016;042083 2015/071339 In one embodiment, there is ed an FcRn binding dimer, wherein at least one of said first and second monomer units comprises sequence iii) or v) wherein Xe is D. In one embodiment, Xe in sequence iii) or v) is E. In one embodiment, Xe in sequence iii) or v) is S.
In one embodiment, there is provided an FcRn binding dimer, wherein at least one of said first and second monomer units comprises ce iii) or v) wherein dee is selected from EE, ES, SD, SE and SS. In one embodiment, dee in sequence iii) or v) is ES. In one embodiment, dee in sequence iii) or v) is SE. In one embodiment, dee in sequence iii) or v) is SD.
In one embodiment, there is provided an FcRn binding dimer, wherein at least one of said first and second monomer units comprises sequence iii) or v) wherein Xf is A. In one embodiment, Xf in ce iii) or v) is S.
In one embodiment, there is ed an FcRn binding dimer, wherein at least one of said first and second monomer units comprises sequence iii) or v), wherein Xa is A; Xb is N; XC is A and Xf is A.
In one embodiment, in sequence iii) or v), X3 is S; Xb is E; XC is A and X is A.
In one ment, in sequence iii) or v), X3 is A; Xb is N; XC is C and Xf is A.
In one embodiment, in sequence iii) or v), X3 is S; Xb is E; XC is S and Xf IS S.
In one embodiment, in sequence iii) or v), X3 is S; Xb is E; XC is C and Xf IS S.
In one embodiment, in sequence iii) or v), X3 is A; Xb is N; XC is A; dee is ND and Xf is A.
In one embodiment, in sequence iii) or v), X3 is S; Xb is E; XC is A; dee is ND and Xf is A.
In one embodiment, in sequence iii) or v), X3 is A; Xb is N; XC is C; dee is ND and Xf is A.
In one embodiment, in sequence iii) or v), X3 is S; Xb is E; XC is S; dee is ND and Xf is S.
In one embodiment, in sequence iii) or v), X3 is S; Xb is E; XC is C; dee is ND and Xf is S.
In one embodiment, in sequence iii) or v), X3 is A; Xb is N; XC is A; dee is SE and Xf is A.
In one embodiment, in sequence iii) or v), X3 is S; Xb is E; XC is A; dee is SE and Xf is A.
In one ment, in sequence iii) or v), Xa is A; Xb is N; XC is C; dee is SE and Xf is A.
In one embodiment, in sequence iii) or v), Xa is S; Xb is E; XC is S; dee is SE and Xf is S.
In one embodiment, in sequence iii) or v), Xa is S; Xb is E; XC is C; dee is SE and Xf is S.
In one ment, in sequence iii) or v), Xa is A; Xb is N; XC is A; dee is ES and Xf is A.
In one embodiment, in sequence iii) or v), Xa is S; Xb is E; XC is A; dee is ES and Xf is A.
In one embodiment, in sequence iii) or v), Xa is A; Xb is N; XC is C; dee is ES and Xf is A.
In one ment, in sequence iii) or v), Xa is S; Xb is E; XC is S; dee is ES and Xf is S.
In one embodiment, in ce iii) or v), Xa is S; Xb is E; XC is C; dee is ES and Xf is S.
In one embodiment, in sequence iii) or v), X3 is A; Xb is N; XC is A; dee is SD and Xf is A.
In one embodiment, in sequence iii) or v), X3 is S; Xb is E; XC is A; dee is SD and Xf is A.
In one embodiment, in sequence iii) or v), X3 is A; Xb is N; XC is C; dee is SD and Xf is A.
In one embodiment, in sequence iii) or v), Xa is S; Xb is E; XC is S; dee is SD and Xf is S.
In one embodiment, in sequence iii) or v), X6] is S; Xb is E; XC is C; dee is SD and Xf is S.
In one embodiment of the FcRn binding dimer according to the first aspect, at least one of said first and second monomer units comprises a Bil/10d according to sequence iii) corresponding to the sequence from position 7 to on 55 in a sequence selected from the group consisting of SEQ ID NO:1-353, SEQ ID NO:358 and SEQ ID NO:360-364. Hence, in one embodiment of the FcRn binding dimer according to the first aspect, at least one of said first and second monomer units comprises a BMod corresponding to the sequence from position 7 to position 55 in a sequence selected from the group consisting of SEQ ID NO:1-353, SEQ ID NO:358 and SEQ ID NO:360-364, such as the group consisting of SEQ ID NO:17-352 and SEQ ID WO 42083 NO:360-364. In one embodiment, said BMod corresponds to the sequence from position 7 to position 55 in a sequence selected from the group ting of SEQ ID NO:1-15, SEQ ID NO:17-140,SEQ ID NO:353, SEQ ID NO:358 and SEQ ID NO:360-364, such as the group consisting of SEQ ID NO:17-140 and SEQ ID NO:360-364. In one embodiment, said BMod corresponds to the sequence from position 7 to on 55 in a sequence selected from the group consisting of SEQ ID NO:1-2,SEQ ID NO:17-140, SEQ ID NO:358 and SEQ ID NO:360-364. In one embodiment, said BMod corresponds to the sequence from position 7 to position 55 in a sequence selected from the group consisting of SEQ ID NO:1-2, SEQ ID NO:17-92, SEQ ID NO:94-103, SEQ ID NO:105-125, SEQ ID NO:127-140, SEQ ID NO:358 and SEQ ID NO:360-364, such as the group consisting of SEQ ID NO:17-92, SEQ ID NO:94-103, SEQ ID NO:105-125, SEQ ID -140 and SEQ ID NO:360-364. In one embodiment, said BMod corresponds to the sequence from position 7 to position 55 in a sequence selected from the group consisting of SEQ ID NO:1-8, SEQ ID NO:13, SEQ ID NO:19-20, SEQ ID NO:23, SEQ ID NO:28, SEQ ID NO:41, SEQ ID NO:44, SEQ ID NO:65, SEQ ID NO:70, SEQ ID NO:73, SEQ ID NO:75-77, SEQ ID NO:353, SEQ ID NO:358 and SEQ ID NO:360-364, such as the group consisting of SEQ ID NO:19-20, SEQ ID NO:23, SEQ ID NO:28, SEQ ID NO:41, SEQ ID NO:44, SEQ ID NO:65, SEQ ID NO:70, SEQ ID NO:73, SEQ ID NO:75-77 and SEQ ID NO:360-364. In another embodiment, said BMod corresponds to the sequence from on 7 to position 55 in a sequence ed from the group consisting of SEQ ID N021, SEQ ID NO:20, SEQ ID NO:23, SEQ ID NO:28, SEQ ID NO:41, SEQ ID NO:44, SEQ ID NO:65, SEQ ID NO:73, SEQ ID NO:75-77, SEQ ID NO:358 and SEQ ID NO:360-364, such as the group ting of SEQ ID NO:20, SEQ ID NO:23, SEQ ID NO:28, SEQ ID NO:41, SEQ ID NO:44, SEQ ID NO:65, SEQ ID NO:73, SEQ ID NO:75-77 and SEQ ID NO:360-364. In another embodiment, said BMod corresponds to the sequence from on 7 to position 55 in a sequence selected from the group consisting of SEQ ID NO:1, SEQ ID NO:20, SEQ ID NO:23, SEQ ID NO:28, SEQ ID NO:41, SEQ ID NO:44, SEQ ID NO:65, SEQ ID NO:73 and SEQ ID NO:75-77, such as the group ting of SEQ ID NO:20, SEQ ID NO:23, SEQ ID NO:28, SEQ ID NO:41, SEQ ID NO:44, SEQ ID NO:65, SEQ ID NO:73 and SEQ ID NO:75—77. In r embodiment, said BMod corresponds to the sequence from position 7 to position 55 in a sequence selected from the group consisting of SEQ ID NO:1, SEQ ID NO:23, SEQ ID NO:28, SEQ ID NO:41, SEQ ID NO:44, SEQ ID NO:65, SEQ ID NO:73 and SEQ ID 77, such as the group consisting of SEQ ID NO:23, SEQ ID NO:28, SEQ ID NO:41, SEQ ID NO:44, SEQ ID NO:65, SEQ ID NO:73 and SEQ ID NO:75-77. In yet another embodiment, said BMod corresponds to the ce from position 7 to position 55 in a sequence selected from the group consisting of SEQ ID NO:1, SEQ ID NO:20, SEQ ID NO:23, SEQ ID N 0:41, SEQ ID NO:44, SEQ ID NO:65, SEQ ID NO:75, SEQ ID NO:77, SEQ ID NO:358 and SEQ ID NO:360-364, such as the group consisting of, SEQ ID NO:20, SEQ ID NO:23, SEQ ID NO:41, SEQ ID NO:44, SEQ ID NO:65, SEQ ID NO:75 and SEQ ID -364. In r embodiment, said BMod corresponds to the sequence from position 7 to position 55 in a sequence selected from the group consisting of SEQ ID NO:20, SEQ ID NO:23, SEQ ID NO:41, SEQ ID NO:44, SEQ ID NO:65, SEQ ID NO:75 and SEQ ID - 364. In yet another embodiment, said BMod corresponds to the sequence from position 7 to position 55 in a sequence selected from the group consisting of SEQ ID NO:1, SEQ ID NO:20, SEQ ID NO:23, SEQ ID NO:41, SEQ ID NO:44, SEQ ID NO:65, SEQ ID NO:75, SEQ ID NO:358 and SEQ ID NO:360-364, such as the group consisting of SEQ ID NO:20, SEQ ID NO:23, SEQ ID NO:41, SEQ ID NO:44, SEQ ID NO:75 and SEQ ID NO:360-364. In yet another embodiment, said BMod corresponds to the sequence from position 7 to position 55 in a sequence selected from the group consisting of SEQ ID NO:1, SEQ ID NO:23, SEQ ID NO:44, SEQ ID NO:65, SEQ ID NO:75 and SEQ ID NO:77, such as the group consisting of SEQ ID NO:1, SEQ ID NO:23, SEQ ID NO:44, SEQ ID NO:65 and SEQ ID NO:75. In another ment, said BMod corresponds to the sequence from position 7 to position 55 in a sequence selected from the group consisting of SEQ ID NO:20, SEQ ID NO:23, SEQ ID NO:41, SEQ ID NO:44, SEQ ID NO:65, SEQ ID NO:75 and SEQ ID NO:77, such as the group consisting of SEQ ID NO:20, SEQ ID NO:23, SEQ ID NO:41, SEQ ID NO:44, SEQ ID NO:65 and SEQ ID NO:75, such as the group consisting of SEQ ID NO:20, SEQ ID NO:23, SEQ ID NO:41, SEQ ID NO:44 and SEQ ID NO:75. In yet r embodiment, said BMod corresponds to the sequence from position 7 to position 55 in a sequence selected from the group consisting of SEQ ID NO:23, SEQ ID NO:44, SEQ ID NO:65, SEQ ID NO:75 and SEQ ID NO:77, such as the group consisting of SEQ ID NO:23, SEQ ID NO:44, SEQ ID NO:65 and SEQ ID NO:75. In one embodiment, said BMod corresponds to the sequence from position 7 to position 55 in a sequence selected from the group consisting of SEQ ID NO:1, SEQ ID NO:23 SEQ ID NO:75, SEQ ID NO:358, SEQ ID NO:361 and SEQ ID NO:364, such as the group consisting of SEQ ID NO:23, SEQ ID NO:75, SEQ ID NO:361 and SEQ ID NO:364. In one ment, said BMod corresponds to the ce from position 7 to position 55 in a sequence selected from the group consisting of SEQ ID NO:1, SEQ ID N023 and SEQ ID NO:75, such as the group consisting of SEQ ID N023 and SEQ ID NO:75.
In one ment, said BMod corresponds to the sequence from position 7 to position 55 in a sequence selected from the group consisting of SEQ ID NO:20, SEQ ID NO:41, SEQ ID NO:44, SEQ ID NO:360, SEQ ID NO:362 and SEQ ID NO:363, such as the group consisting of SEQ ID NO:20, SEQ ID NO:41, SEQ ID NO:360 and SEQ ID NO:362; the group consisting of SEQ ID NO:20, SEQ ID NO:44, SEQ ID NO:360 and SEQ ID NO:363; or the group consisting of SEQ ID NO:41, SEQ ID NO:44, SEQ ID NO:362 and SEQ ID NO:363.
In one embodiment, said BMod corresponds to the sequence from position 7 to position 55 in a sequence selected from the group consisting of SEQ ID NO:1, SEQ ID NO:23, SEQ ID NO:44, SEQ ID NO:358, SEQ ID NO:361 and SEQ ID NO:363, such as the group consisting of SEQ ID NO:23, SEQ ID NO:44, SEQ ID NO:361 and SEQ ID NO:363. In one embodiment, said BMod corresponds to the sequence from position 7 to position 55 in a sequence selected from the group ting of SEQ ID NO:1, SEQ ID N023 and SEQ ID NO:44, such as the group consisting of SEQ ID N023 and SEQ ID NO:44. In one embodiment, said BMod corresponds to the sequence from position 7 to position 55 in SEQ ID NO:1 or SEQ ID NO:23 or SEQ ID NO:44.
In one embodiment, said BMod corresponds to the sequence from position 7 to position 55 in SEQ ID NO:20 or SEQ ID NO:41 or SEQ ID NO:44. In one embodiment, said BMod corresponds to the ce from position 7 to position 55 in SEQ ID NO:360 or SEQ ID NO:362 or SEQ ID NO:363.
In one embodiment of the FcRn binding dimer as disclosed herein, both of said first and second monomer units comprise a BMod corresponding to the sequence from position 7 to position 55 in a sequence ed from one of the groups defined above. In one embodiment, said group consists of SEQ ID NO:1, SEQ ID NO:20, SEQ ID NO:23, SEQ ID NO:41, SEQ ID NO:44, SEQ ID NO:65, SEQ ID NO:75, SEQ ID NO:358 and SEQ ID NO:360- 364. In one ment, said group consists of SEQ ID NO:20, SEQ ID NO:23, SEQ ID NO:41, SEQ ID NO:44, SEQ ID NO:75 and SEQ ID N02360- W0 2016;042083 364. In one ment, said group consists of SEQ ID NO:20, SEQ ID NO:23, SEQ ID NO:41, SEQ ID NO:44 and SEQ ID NO:75. In another embodiment, said group consists of SEQ ID NO:360-364. In one embodiment, said group consists of SEQ ID NO:1, SEQ ID NO:23, SEQ ID NO:44, SEQ ID NO:65 and SEQ ID NO:75. In another embodiment, said group consists of SEQ ID N021, SEQ ID N023 and SEQ ID NO:44. In one embodiment, said group consists ofSEQ ID NO:20, SEQ ID NO:41, SEQ ID NO:44, SEQ ID NO:360, SEQ ID NO:362 and SEQ ID NO:363. In another embodiment, said group consists of SEQ ID NO:20, SEQ ID NO:41 and SEQ ID NO:44. In another embodiment, said group consists of SEQ ID NO:360, SEQ ID NO:362 and SEQ ID . In one particular embodiment, both said first and second monomer units comprise a BMW corresponding to the sequence from position 7 to position 55 in SEQ ID NO:1 or SEQ ID NO:358.
In one embodiment, said BMod corresponds to the sequence from position 7 to position 55 in SEQ ID NO:20 or SEQ ID NO:360. In one embodiment, said BMod corresponds to the sequence from position 7 to position 55 in SEQ ID NO:23 or SEQ ID . In one embodiment, said BMod corresponds to the sequence from position 7 to position 55 in SEQ ID NO:41 or SEQ ID NO:362.
In one embodiment, said BMod corresponds to the sequence from position 7 to position 55 in SEQ ID NO:44 or SEQ ID . In one embodiment, said BMod corresponds to the sequence from on 7 to position 55 in SEQ ID NO:75 or SEQ ID NO:364.
Also, in a further embodiment, there is provided an FcRn binding dimer as d above, n at least one of said first and second monomer units comprises a sequence selected from the group consisting of: vii) YAK-[BM]-DPSQS SELLXC EAKKL NDSQA P; wherein [BM] is an FcRn binding motif as d above and Xc is selected from A, S and C; and viii) an amino acid sequence which has at least 94 % ty to a sequence defined by vii).
In another embodiment, there is provided an FcRn binding dimer as defined above, wherein at least one of said first and second r units comprises a sequence selected from the group consisting of: ix) FAK-[Bll/I]-DPSQS SELLXC EAKKL SESQA P; wherein [BM] is an FcRn binding motif as defined above and XC is selected from A, S and C; and x) an amino acid sequence which has at least 94 % ty to a sequence defined by ix).
In one embodiment, XC in the sequence defined by ix) is 8.
Alternatively, there is ed an FcRn binding dimer as defined above, wherein at least one of said first and second monomer units comprises a sequence selected from the group consisting of: xi) FNK-[BM]-DPSQS ANLLXC EAKKL NDAQA P; wherein [BM] is an FcRn binding motif as defined above and XC is selected from A and C; and xii) an amino acid sequence which has at least 94 % identity to a sequence defined by xi).
As discussed above, polypeptides comprising minor s as compared to the above amino acid sequences that do not largely affect the tertiary structure and the function thereof are also within the scope of the t disclosure. Thus, in some ments, the FcRn binding dimer as defined above may comprise a sequence viii), x) or xii) which is at least 96 %, such as at least 98 % identical to a sequence defined by vii), ix) or xi), respectively.
In some embodiments of the FcRn binding dimer, at least one of said first and second monomer units may comprise an amino acid sequence ed from ADNNFNK-[BMj-DPSQSANLLSEAKKLNESQAPK; ADNKFNK-[BM]—DPSQSANLLAEAKKLNDAQAPK; ADNKFNK-[BM]—DPSVSKEILAEAKKLNDAQAPK; ADAQQNNFNK-[BM]-DPSQSTNVLGEAKKLNESQAPK; AQHDE-[BM]—DPSQSANVLGEAQKLNDSQAPK; VDNKFNK-[BMj-DPSQSANLLAEAKKLNDAQAPK; K-[BM]-DPSESSELLSEAKKLNKSQAPK; VDAKYAK-[BM]—DPSQSSELLAEAKKLNDAQAPK; VDAKYAK-[BM]—DPSQSSELLAEAKKLNDSQAPK; AEAKYAK-[BM]—DPSQSSELLSEAKKLNDSQAPK; AEAKYAK-[BM]—DPSQSSELLSEAKKLNDSQAP; AEAKFAK-[BM]—DPSQSSELLSEAKKLNDSQAPK; AEAKFAK-[BM]—DPSQSSELLSEAKKLNDSQAP; AEAKYAK-[BM]—DPSQSSELLAEAKKLNDAQAPK; AEAKYAK-[BMJ-DPSQSSELLSEAKKLSESQAPK; AEAKYAK-[BM]—DPSQSSELLSEAKKLSESQAP; AEAKFAK-[BMj-DPSQSSELLSEAKKLSESQAPK; AEAKFAK-[BM]—DPSQSSELLSEAKKLSESQAP; AEAKYAK-[BMJ-DPSQSSELLAEAKKLSEAQAPK; AEAKYAK-[BM]—QPEQSSELLSEAKKLSESQAPK; AEAKYAK-[BMJ-DPSQSSELLSEAKKLESSQAPK; AEAKYAK-[BM]-DPSQSSELLSEAKKLESSQAP; AEAKYAK-[BMJ-DPSQSSELLAEAKKLESAQAPK; AEAKYAK-[BM]—QPEQSSELLSEAKKLESSQAPK; AEAKYAK-[BMJ-DPSQSSELLSEAKKLSDSQAPK; AEAKYAK-[BM]-DPSQSSELLSEAKKLSDSQAP; AEAKYAK-[BMJ-DPSQSSELLAEAKKLSDAQAPK; AEAKYAK-[BMj-QPEQSSELLSEAKKLSDSQAPK; VDAKYAK-[BMj-DPSQSSELLSEAKKLNDSQAPK' K-[BMj-DPSQSSELLSEAKKLNDSQAPK; VDAKYAK-[BMJ-DPSQSSELLAEAKKLNDAQAPK' VDAKYAK-[BMj-DPSQSSELLSEAKKLSESQAPK; VDAKFAK-[BMj-DPSQSSELLSEAKKLSESQAPK; VDAKYAK-[BMj-DPSQSSELLAEAKKLSEAQAPK; VDAKYAK-[BMJ-QPEQSSELLSEAKKLSESQAPK; VDAKYAK-[BMj-DPSQSSELLSEAKKLESSQAPK; K-[BMj-DPSQSSELLAEAKKLESAQAPK; VDAKYAK-[BMJ—QPEQSSELLSEAKKLESSQAPK; K-[BMj-DPSQSSELLSEAKKLSDSQAPK; VDAKYAK-[BMj-DPSQSSELLAEAKKLSDAQAPK; VDAKYAK-[BMj-QPEQSSELLSEAKKLSDSQAPK; VDAKYAK-[BMJ-DPSQSSELLAEAKKLNKAQAPK; AEAKYAK-[BMJ-DPSQSSELLAEAKKLNKAQAPK; and ADAKYAK-[BMJ-DPSQSSELLSEAKKLNDSQAPK; wherein [BM] is an FcRn g motif as defined above.
In one embodiment, at least one of said first and second monomer units of the FcRn binding dimer may comprise an amino acid sequence selected from: xiii) AEAKYAK-[BM]—DPSQSSELLSEAKKLNDSQAPK; wherein [BM] is an FcRn binding motif as defined above; and W0 2016;042083 xiv) an amino acid sequence which has at least 94 % identity to the sequence defined in xiii).
In one embodiment, sequence xiii) is selected from the group ting of SEQ ID NO:354-357, such as in particular selected from the group ting of SEQ ID NO:354 and 357.
In one embodiment, both said first and second r units comprise a ce xiii) selected from the group consisting of SEQ ID NO:354-357, such as in particular selected from the group consisting of SEQ ID NO:354 and 357. In one embodiment, said sequence xiii) is SEQ ID NO:354 in both said first and second monomer units. In one embodiment, said sequence xiii) is SEQ ID NO:357 in both said first and second monomer units.
In one embodiment, at least one of said first and second monomer units of the FcRn binding dimer may comprise an amino acid sequence selected from: xv) AEAKFAK-[BM]—DPSQSSELLSEAKKLSESQAPK; wherein [BM] is an FcRn binding motif as defined above; and xvi) an amino acid sequence which has at least 94 % identity to the ce defined in xv).
In one embodiment, sequence xv) is selected from the group consisting of SEQ ID NO:365-367. In one embodiment, ce xv) is SEQ ID NO:365, SEQ ID NO:366 or SEQ ID NO:367.
In one embodiment, at least one of said first and second monomer units of the FcRn binding dimer may comprise an amino acid sequence selected from: xvii) VDAKYAK-[BM]—DPSQSSELLSEAKKLSESQAPK; wherein [BM] is an FcRn binding motif as defined above; and xviii) an amino acid sequence which has at least 94 % identity to the sequence defined in xvii).
In one ment, sequence xvii) is selected from the group consisting of SEQ ID NO:360-364. In one embodiment, sequence xvii) is SEQ ID NO:360, SEQ ID NO:361. SEQ ID NO:362, SEQ ID NO:363 or SEQ ID NO:364.
In one ment, at least one of said first and second monomer units of the FcRn binding dimer may comprise an amino acid sequence selected from: xix) AEAKYAK-[BM]—RQPESSELLSEAKKLSESQAPK; W0 2016;042083 wherein [BM] is an FcRn binding motif as d above; and xx) an amino acid sequence which has at least 94 % identity to the sequence defined in xix).
In one embodiment, ce xix) is SEQ ID NO:359.
In one embodiment, at least one of said first and second monomer units of the FcRn binding dimer may comprise an amino acid sequence selected from: xxi) VDAKYAK-[BM]—DPSQSSELLSEAKKLNDSQAPK; wherein [BM] is an FcRn binding motif as defined above; and xxii) an amino acid sequence which has at least 94 % identity to the sequence defined in xxi).
Again, polypeptides comprising minor changes as compared to the above amino acid sequences which do not largely affect the tertiary structure and the on thereof are also within the scope of the present disclosure.
Thus, in some embodiments, the FcRn binding dimer as defined above may comprise a sequence xiv), xvi), xviii), xx) or xxii) which is at least 96 %, such as at least 98 % identical to a sequence defined by xiii), xv), xvii), xix) or xxi), tively.
In one embodiment of the FcRn binding dimer according to the first aspect, at least one of said first and second monomer units comprises a sequence xxi) selected from the group consisting of SEQ ID 53, such as the group consisting of SEQ ID NO:17-352. In one embodiment, said ce xxi) is a sequence ed from the group consisting of SEQ ID NO:1-15, SEQ ID NO:17-140 and SEQ ID NO:353, such as the group consisting of SEQ ID NO:17-140. In one embodiment, said sequence xxi) is a sequence selected from the group consisting of SEQ ID NO:1-2 and SEQ ID NO:17-140. In one embodiment, said sequence xxi) is a ce selected from the group consisting of SEQ ID , SEQ ID NO:17-92, SEQ ID NO:94-103, SEQ ID NO:105-125 and SEQ ID NO:127-140, such as the group consisting of SEQ ID NO:17-92, SEQ ID NO:94-103, SEQ ID NO:105-125 and SEQ ID NO:127-140. In one embodiment, said sequence xxi) is a sequence selected from the group consisting of SEQ ID NO:1-8, SEQ ID NO:13, SEQ ID NO:19—20, SEQ ID NO:23, SEQ ID NO:28, SEQ ID NO:41, SEQ ID NO:44, SEQ ID NO:65, SEQ ID NO:70, SEQ ID NO:73, SEQ ID WO 42083 NO:75-77 and SEQ ID NO:353, such as the group consisting of SEQ ID NO:19-20, SEQ ID NO:23, SEQ ID NO:28, SEQ ID NO:41, SEQ ID NO:44, SEQ ID NO:65, SEQ ID NO:70, SEQ ID NO:73 and SEQ ID NO:75-77. In another embodiment, said sequence xxi) is a sequence selected from the group consisting of SEQ ID NO:1, SEQ ID NO:20, SEQ ID NO:23, SEQ ID NO:28, SEQ ID NO:41, SEQ ID NO:44, SEQ ID NO:65, SEQ ID NO:73 and SEQ ID NO:75-77, such as the group consisting of SEQ ID NO:20, SEQ ID NO:23, SEQ ID NO:28, SEQ ID NO:41, SEQ ID NO:44, SEQ ID NO:65, SEQ ID NO:73 and SEQ ID 77. In another embodiment, said ce xxi) is a sequence selected from the group consisting of SEQ ID NO:1, SEQ ID NO:23, SEQ ID NO:28, SEQ ID NO:41, SEQ ID NO:44, SEQ ID NO:65, SEQ ID NO:73 and SEQ ID NO:75-77, such as the group consisting of SEQ ID NO:23, SEQ ID NO:28, SEQ ID NO:41, SEQ ID NO:44, SEQ ID NO:65, SEQ ID NO:73 and SEQ ID NO:75-77. In yet another embodiment, said sequence xxi) is a sequence selected from the group consisting of SEQ ID NO:1, SEQ ID NO:20, SEQ ID NO:23, SEQ ID NO:41, SEQ ID NO:44, SEQ ID NO:65, SEQ ID NO:75 and SEQ ID NO:77, such as the group consisting of SEQ ID NO:1, SEQ ID NO:20, SEQ ID NO:23, SEQ ID NO:41, SEQ ID NO:44, SEQ ID NO:65, SEQ ID NO:75 and SEQ ID NO:77. In yet another embodiment, said sequence xxi) is a ce selected from the group consisting of SEQ ID NO:1, SEQ ID NO:23, SEQ ID NO:44, SEQ ID NO:65, SEQ ID NO:75 and SEQ ID NO:77, such as the group consisting of SEQ ID NO:1, SEQ ID NO:23, SEQ ID NO:44, SEQ ID NO:65 and SEQ ID NO:75. In yet another embodiment, said sequence xxi) is a sequence selected from the group consisting of SEQ ID NO:20, SEQ ID NO:23, SEQ ID NO:41, SEQ ID NO:44, SEQ ID NO:65, SEQ ID NO:75 and SEQ ID NO:77, such as the group consisting of SEQ ID NO:20, SEQ ID NO:23, SEQ ID NO:41, SEQ ID NO:44, SEQ ID NO:65 and SEQ ID NO:75, such as the group ting of SEQ ID NO:20, SEQ ID NO:23, SEQ ID NO:41, SEQ ID NO:44 and SEQ ID NO:75. In yet another embodiment, said sequence xxi) is a sequence selected from the group consisting of SEQ ID NO:23, SEQ ID NO:44, SEQ ID NO:65, SEQ ID NO:75 and SEQ ID NO:77, such as the group consisting of SEQ ID NO:23, SEQ ID NO:44, SEQ ID NO:65 and SEQ ID NO:75. In one embodiment, said sequence xiii) is a sequence selected from the group consisting of SEQ ID NO:1, SEQ ID N023 and SEQ ID NO:75, such as the group consisting of SEQ ID N023 and SEQ ID NO:75. In one embodiment, said sequence xxi) is a sequence ed from the group consisting of SEQ ID NO:20, SEQ ID NO:41 and SEQ ID NO:44, such as the group ting of SEQ ID N020 and SEQ ID NO:41; the group consisting of SEQ ID N020 and SEQ ID NO:44; or the group consisting of SEQ ID NO:41 and SEQ ID NO:44. In one embodiment, said sequence xxi) is a sequence selected from the group ting of SEQ ID NO:1, SEQ ID N023 and SEQ ID NO:44, such as the group consisting of SEQ ID N023 and SEQ ID NO:44. In one embodiment, said sequence xxi) is SEQ ID NO:1, or is SEQ ID NO:20, or is SEQ ID NO:23, or is SEQ ID NO:41, or is SEQ ID NO:44.
In one embodiment of the FcRn binding dimer as disclosed herein, both said first and second monomer units comprise a sequence xxi) or xiii) selected from one of the groups defined above. In one embodiment, said group consists of SEQ ID NO:1, SEQ ID NO:23, SEQ ID NO:44, SEQ ID NO:65, SEQ ID NO:75, SEQ ID NO:354 and SEQ ID NO:357, such as the group consisting of SEQ ID NO:1, SEQ ID NO:23, SEQ ID NO:44, SEQ ID NO:354 and SEQ ID NO:357, such as the group consisting of SEQ ID NO:1, SEQ ID N023 and SEQ ID NO:44 or the group consisting of SEQ ID NO:23, SEQ ID NO:44, SEQ ID NO:354 and SEQ ID NO:357.
In one embodiment of the FcRn g dimer as disclosed herein, both said first and second monomer units comprise a sequence xiii), xv), xvii), xix) or xxi) selected from one of the groups defined above.
In one ment, said group consists of SEQ ID NO:1, SEQ ID NO:20; SEQ ID NO:23, SEQ |D:41; SEQ ID NO:44, SEQ ID NO:65, SEQ ID NO:75, SEQ ID NO:354, SEQ ID NO:357 and SEQ ID NO:360-367, such as the group consisting of SEQ ID NO:20; SEQ ID NO:23, SEQ ID:41; SEQ ID NO:44, SEQ ID NO:75, SEQ ID NO:357 and SEQ ID NO:360-367, such as the group consisting of SEQ ID NO:20, SEQ ID NO:41, SEQ ID NO:44, SEQ ID NO:357, SEQ ID NO:360, SEQ ID , SEQ ID NO:363, SEQ ID NO:365, SEQ ID NO:366 and SEQ ID NO:367, such as the group consisting of SEQ ID NO:357, SEQ ID NO:360, SEQ ID NO:362, SEQ ID NO:363, SEQ ID NO:365, SEQ ID NO:366 and SEQ ID NO:367. In one particular embodiment, both said first and second monomer units se a sequence xxi) selected from the group ting ofSEQ ID NO:1, SEQ ID NO:23, SEQ ID NO:44, SEQ ID NO:65 and SEQ ID NO:75, such as the group consisting of SEQ ID NO:23, SEQ ID NO:44, SEQ ID NO:65 and SEQ ID NO:75, such as the group consisting ofSEQ ID N023 and SEQ ID NO:44. In one particular embodiment, both said first and second monomer units comprise a sequence xxi) corresponding to SEQ ID N021. In one embodiment, said sequence xxi) is SEQ ID N0220. In one ment, said sequence xxi) is SEQ ID N0223.
In one embodiment, said sequence xxi) is SEQ ID N0241. In one embodiment, said sequence xxi) is SEQ ID N0244. In one embodiment, said sequence xxi) is SEQ ID N0275.
In another embodiment, both said first and second monomer units comprise a sequence xiii) corresponding to SEQ ID N0:354.
In one particular embodiment, both said first and second monomer units comprise a sequence xix) ponding to SEQ ID N02360. In one embodiment, said sequence xix) is SEQ ID N02361. In one embodiment, said sequence xix) is SEQ ID . In one embodiment, said sequence xix) is SEQ ID N02363. In one embodiment, said sequence xix) is SEQ ID NO:364.
In another particular embodiment, both said first and second monomer units comprise a sequence xv) corresponding to SEQ ID N0:365. In one embodiment, said sequence xv) is SEQ ID N02366. In one embodiment, said sequence xv) is SEQ ID .
In a specific ment of the FcRn binding dimer, the first and second monomer units comprise SEQ ID N021 and SEQ ID NO:1; SEQ ID N021 and SEQ ID N0223; SEQ ID N021 and SEQ ID N0244; SEQ ID N021 and SEQ ID N0265; SEQ ID N021 and SEQ ID N0275; SEQ ID N021 and SEQ ID N0:354; SEQ ID N021 and SEQ ID N0:357;SEQ ID N0223 and SEQ ID N0223; SEQ ID N0223 and SEQ ID N0244; SEQ ID N0223 and SEQ ID N0265; SEQ ID N0223 and SEQ ID N0275; SEQ ID N0223 and SEQ ID N02354; SEQ ID N0223 and SEQ ID N02357; SEQ ID N0244 and SEQ ID N0244; SEQ ID N0244 and SEQ ID N0265; SEQ ID N0244 and SEQ ID N0275; SEQ ID N0244 and SEQ ID N02354; SEQ ID N0244 and SEQ ID N02357; SEQ ID N0265 and SEQ ID N0265; SEQ ID N0265 and SEQ ID N0275; SEQ ID N0265 and SEQ ID N02354; SEQ ID N0265 and SEQ ID N02357; SEQ ID N0275 and SEQ ID N02354; SEQ ID N0275 and SEQ ID N02357; SEQ ID N0:354 and SEQ ID N02354; SEQ ID N0:354 and SEQ ID N02357; or SEQ ID N02357 and SEQ ID N02357, respectively. In one embodiment, the first and second r units comprise SEQ ID N021 and SEQ ID NO:1; SEQ ID N021 and SEQ ID N0223; SEQ ID N021 and SEQ ID N0244; SEQ ID N021 and SEQ ID N02354; SEQ ID N021 and SEQ ID N02357; SEQ ID N0223 and SEQ ID N0223; SEQ ID N0223 and SEQ ID N0244; SEQ ID N0223 and SEQ ID N02354; SEQ ID N0223 and SEQ ID 2015/071339 NO:357; SEQ ID NO:44 and SEQ ID NO:44; SEQ ID NO:44 and SEQ ID NO:354; SEQ ID NO:44 and SEQ ID NO:357; SEQ ID NO:354 and SEQ ID NO:354; SEQ ID NO:354 and SEQ ID NO:357; or SEQ ID NO:357 and SEQ ID NO:357, respectively. In another embodiment, the first and second monomer units comprise SEQ ID NO:1 and SEQ ID NO:1; SEQ ID N023 and SEQ ID NO:23; SEQ ID NO:44 and SEQ ID NO:44; SEQ ID NO:354 and SEQ ID NO:354; or SEQ ID NO:357 and SEQ ID , respectively. In yet another embodiment, the first and second monomer units comprise SEQ ID NO:44 and SEQ ID NO:44; or SEQ ID NO:357 and SEQ ID NO:357, respectively.
In a specific embodiment of the FcRn binding dimer, the first and second monomer units comprise SEQ ID NO:1 and SEQ ID NO:1; SEQ ID NO:1 and SEQ ID NO:20; SEQ ID NO:1 and SEQ ID NO:23; SEQ ID NO:1 and SEQ ID NO:41; SEQ ID NO:1 and SEQ ID NO:44; SEQ ID NO:1 and SEQ ID NO:65; SEQ ID NO:1 and SEQ ID NO:75; SEQ ID NO:1 and SEQ ID NO:354; SEQ ID NO:1 and SEQ ID ; SEQ ID NO:1 and SEQ ID NO:365; SEQ ID NO:1 and SEQ ID NO:366; SEQ ID NO:1 and SEQ ID NO:367; SEQ ID NO:20 and SEQ ID NO:20; SEQ ID NO:20 and SEQ ID NO:23; SEQ ID NO:20 and SEQ ID NO:41; SEQ ID NO:20 and SEQ ID NO:44; SEQ ID NO:20 and SEQ ID NO:357; SEQ ID NO:20 and SEQ ID NO:365; SEQ ID NO:20 and SEQ ID NO:366; SEQ ID NO:20 and SEQ ID NO:367; SEQ ID N023 and SEQ ID NO:23; SEQ ID N023 and SEQ ID NO:41; SEQ ID N023 and SEQ ID NO:44; SEQ ID N023 and SEQ ID NO:65; SEQ ID N023 and SEQ ID NO:75; SEQ ID N023 and SEQ ID NO:354; SEQ ID N023 and SEQ ID NO:357; SEQ ID N023 and SEQ ID NO:365; SEQ ID N023 and SEQ ID NO:366; SEQ ID N023 and SEQ ID NO:367; SEQ ID NO:41 and SEQ ID NO:41; SEQ ID NO:41 and SEQ ID NO:44; SEQ ID NO:41 and SEQ ID NO:357; SEQ ID NO:41 and SEQ ID NO:365; SEQ ID NO:41 and SEQ ID NO:366; SEQ ID NO:41 and SEQ ID NO:367; SEQ ID NO:44 and SEQ ID NO:44; SEQ ID NO:44 and SEQ ID NO:65; SEQ ID NO:44 and SEQ ID NO:75; SEQ ID NO:44 and SEQ ID NO:354; SEQ ID NO:44 and SEQ ID NO:357; SEQ ID NO:44 and SEQ ID NO:365; SEQ ID NO:44 and SEQ ID ; SEQ ID NO:44 and SEQ ID NO:367; SEQ ID NO:65 and SEQ ID NO:65; SEQ ID NO:65 and SEQ ID NO:75; SEQ ID NO:65 and SEQ ID NO:354; SEQ ID NO:65 and SEQ ID NO:357; SEQ ID NO:75 and SEQ ID NO:354; SEQ ID NO:75 and SEQ ID NO:357; SEQ ID NO:354 and SEQ ID NO:354; SEQ ID NO:354 and SEQ ID NO:357; SEQ ID NO:357 and SEQ ID NO:357; SEQ ID NO:357 and SEQ ID NO:365; SEQ ID NO:357 and SEQ ID NO:366; SEQ ID NO:357 and SEQ ID NO:367; SEQ ID NO:365 and SEQ ID NO:365; SEQ ID NO:365 and SEQ ID NO:366; SEQ ID NO:365 and SEQ ID ; SEQ ID NO:366 and SEQ ID ; SEQ ID NO:366 and SEQ ID NO:367; or SEQ ID NO:367 and SEQ ID NO:367, respectively. In one embodiment, the first and second monomer units comprise SEQ ID NO:1 and SEQ ID NO:1; SEQ ID NO:1 and SEQ ID NO:20; SEQ ID NO:1 and SEQ ID NO:23; SEQ ID NO:1 and SEQ ID NO:41; SEQ ID NO:1 and SEQ ID NO:44; SEQ ID NO:1 and SEQ ID NO:354; SEQ ID NO:1 and SEQ ID NO:357; SEQ ID NO:1 and SEQ ID NO:365; SEQ ID NO:1 and SEQ ID NO:366; SEQ ID NO:1 and SEQ ID NO:367; SEQ ID N020 and SEQ ID NO:20; SEQ ID N020 and SEQ ID NO:23; SEQ ID N020 and SEQ ID NO:41; SEQ ID N020 and SEQ ID NO:44; SEQ ID N020 and SEQ ID NO:357; SEQ ID N020 and SEQ ID NO:365; SEQ ID N020 and SEQ ID NO:366; SEQ ID N020 and SEQ ID NO:367; SEQ ID N023 and SEQ ID NO:23; SEQ ID N023 and SEQ ID NO:41; SEQ ID N023 and SEQ ID NO:44; SEQ ID N023 and SEQ ID NO:354; SEQ ID N023 and SEQ ID NO:357; SEQ ID N023 and SEQ ID NO:365; SEQ ID N023 and SEQ ID NO:366; SEQ ID N023 and SEQ ID NO:367; SEQ ID NO:41 and SEQ ID NO:41; SEQ ID NO:41 and SEQ ID NO:44; SEQ ID NO:41 and SEQ ID NO:357; SEQ ID NO:41 and SEQ ID ; SEQ ID NO:41 and SEQ ID NO:366; SEQ ID NO:41 and SEQ ID NO:367; SEQ ID NO:44 and SEQ ID NO:44; SEQ ID NO:44 and SEQ ID ; SEQ ID NO:44 and SEQ ID NO:357; SEQ ID NO:44 and SEQ ID NO:365; SEQ ID NO:44 and SEQ ID NO:366; SEQ ID NO:44 and SEQ ID NO:367; SEQ ID NO:354 and SEQ ID ; SEQ ID NO:354 and SEQ ID NO:357; SEQ ID NO:357 and SEQ ID NO:357; SEQ ID NO:357 and SEQ ID NO:365; SEQ ID NO:357 and SEQ ID NO:366; SEQ ID NO:357 and SEQ ID NO:367; SEQ ID NO:365 and SEQ ID NO:365; SEQ ID NO:365 and SEQ ID NO:366; SEQ ID NO:365 and SEQ ID ; SEQ ID NO:366 and SEQ ID NO:366; SEQ ID NO:366 and SEQ ID NO:367; or SEQ ID NO:367 and SEQ ID NO:367, respectively. In another embodiment, the first and second monomer units comprise SEQ ID NO:1 and SEQ ID NO:1; SEQ ID N020 and SEQ ID NO:20; SEQ ID N023 and SEQ ID NO:23; SEQ ID NO:41 and SEQ ID NO:41; SEQ ID NO:44 and SEQ ID NO:44; SEQ ID NO:354 and SEQ ID NO:354; SEQ ID NO:357 and SEQ ID NO:357; SEQ ID NO:365 and SEQ ID W0 2016;042083 NO:365; SEQ ID NO:366 and SEQ ID NO:366; or SEQ ID NO:367 and SEQ ID , respectively. In yet another embodiment, the first and second monomer units comprise SEQ ID N020 and SEQ ID NO:20; SEQ ID NO:41 and SEQ ID NO:41; SEQ ID NO:44 and SEQ ID NO:44; SEQ ID NO:357 and SEQ ID NO:357; SEQ ID NO:365 and SEQ ID ; SEQ ID NO:366 and SEQ ID NO:366; or SEQ ID NO:367 and SEQ ID NO:367, respectively.
In yet another ment, the first and second monomer units comprise SEQ ID NO:365 and SEQ ID NO:365; SEQ ID NO:366 and SEQ ID NO:366; or SEQ ID NO:367 and SEQ ID NO:367, respectively.
For the sake of clarity, the designation of first and second monomer units as used throughout the present disclosure is made for clarity reasons to distinguish between them, and is not intended to refer to the actual order of the r units in the polypeptide chain of the FcRn binding dimer. Thus, for example, said first monomer unit may appear N—terminally or C-terminally in a polypeptide chain, with respect to said second monomer unit.
As the skilled person understands, the construction of a fusion protein often involves using linkers between functional moieties to be fused. The skilled person is aware of different kinds of linkers with different properties, such as flexible amino acid linkers, rigid amino acid linkers and cleavable amino acid linkers. Linkers have been used to for example se ity or improve folding of fusion proteins, to se expression, improve biological activity, enable targeting and alter pharmacokinetics of fusion ns.
Thus, in one embodiment of the first aspect, there is provided an FcRn binding dimer as defined herein, wherein said linker is selected from the group ting of flexible amino acid linkers, rigid amino acid linkers and cleavable amino acid linkers. In one embodiment of an FcRn binding dimer as defined herein, said linker is arranged between the first monomeric unit and the second monomeric unit. The d person will appreciate that the presence of a linker arranged between the first monomeric unit and the second monomeric unit does not exclude the presence of onal linkers.
Flexible linkers are often used in the art when the joined domains require a certain degree of movement or interaction, and may be particularly useful in some embodiments of the FcRn binding dimer. Such linkers are generally ed of small, non-polar (for example G) or polar (for example W0 2016;042083 S or T) amino acids. Some flexible linkers primarily consist of hes of G and 8 residues, for e (GGGGS)p and (SSSSG)p. ing the copy number “p” allows for optimization of the linker in order to achieve riate separation between the functional moieties or to maintain necessary inter- moiety interaction. Apart from G and S linkers, other flexible linkers are known in the art, such as G and S linkers ning additional amino acid residues, such as T, A, K and E, to maintain flexibility, as well as polar amino acid residues to improve solubility.
Additional non-limiting examples of linkers include GGGGSLVPRGSGGGGS, (GS)3, (GS)4, (GS)8, GGSGGHMGSGG, GGSGGSGGSGG, GGSGG, GGSGGGGG, GGGSEGGGSEGGGSEGGG, AAGAATAA, GGGGG, GGSSG, GSGGGTGGGSG, GSGGGTGGGSG, GT, GSGSGSGSGGSG, GSGGSGGSGGSGGS and SGGSGGSG.
The skilled person is aware of other suitable linkers.
In one embodiment, said linker is a flexible linker comprising glycine (G), serine (8) and/or threonine (T) residues. In one embodiment, said linker has a general formula selected from (GnSm)p and (SnGm)p, wherein, independently, n = 1—7, m = 0—7, n + m S 8 and p = 1-7. In one embodiment, n = 1-5. In one embodiment, m = 0-5. In one embodiment, p = 1-5. In a more specific embodiment, n = 4, m = 1 and p = 1-4. In one embodiment, said linker is selected from the group consisting of 84G, (S4G)3 and (S4G)4_ In one ment, said linker is selected from the group ting of G8, G48 and (G4S)3. In one particular embodiment, said linker is G48 and in another embodiment said linker is (G4S)3.
The terms “FcRn binding” and ”binding affinity for FcRn” as used in this ication refer to a property of a polypeptide which may be tested for example by the use of surface plasmon resonance (SPR) technology or ELISA.
For e as described in the examples below, FcRn binding affinity may be tested in an experiment in which FcRn, or a correctly folded fragment thereof, is immobilized on a sensor chip of the instrument, and the sample containing the polypeptide to be tested is passed over the chip. Alternatively, the polypeptide to be tested is immobilized on a sensor chip of the ment, and a sample ning FcRn, or a correctly folded fragment thereof, is passed over the chip. The skilled person may then interpret the results obtained by such experiments to establish at least a qualitative measure of W0 2016;042083 the binding affinity of the polypeptide for FcRn. If a quantitative measure is desired, for example to determine a KD value for the interaction, surface n resonance methods may also be used. Binding values may for example be defined in a Biacore (GE Healthcare) or ProteOn XPR 36 (Bio- Rad) instrument. FcRn is suitably immobilized on a sensor chip of the instrument, and samples of the polypeptide whose affinity is to be determined are prepared by serial dilution and injected in random order. KD values may then be calculated from the results using for example the 1:1 Langmuir binding model of the BlAevaluation 4.1 software, or other suitable software, provided by the instrument manufacturer.
Alternatively, as described in the examples below, FcRn g affinity may be tested in an experiment in which samples of the polypeptide are ed on antibody coated ELISA plates, and biotinylated FcRn is added followed by streptavidin conjugated HRP. TMB substrate is added and the absorbance at 450 nm is measured using a multi-well plate reader, such as Victor3 (Perkin . The skilled person may then interpret the results obtained by such experiments to establish at least a qualitative measure of the binding affinity of the polypeptide for FcRn. If a quantitative measure is desired, for example to determine the KB value (the half maximal effective concentration) for the interaction, ELISA may also be used. The response of the polypeptides against a dilution series of biotinylated FcRn are measured using ELISA as described above. The skilled person may then interpret the results obtained by such experiments and KD values may be calculated from the results using for example GraphPad Prism 5 and non—linear regression.
Alternatively, affinity for FcRn may also be studied indirectly by looking at the ability of an FcRn binding ptide to block binding of IgG to FcRn.
Thus, a d person would iate that the ability of an FcRn binding ptide to block said binding correlates with the binding ty of the FcRn binding polypeptide to FcRn, provided that the FcRn binding dimer interacts with FcRn at the same, or an at least partially overlapping, region of FcRn as IgG. Thus, the higher the capacity of binding of the polypeptide to FcRn, the better the y to block IgG binding to FcRn.
The d person would also iate that interaction of an FcRn binding polypeptide and FcRn can be evaluated by FACS (Fluorescence— activated cell sorting) analysis, wherein the ed mean fluorescence intensity (MFI) value is an indirect readout of the strength of binding of a tested polypeptide ve to other tested polypeptides in the same W0 42083 experiment. Thus, a higher MFI-value correlates to a higher relative affinity and a lower MFI-value correlates to a lower relative affinty.
As used herein, the term “higher binding capacity” in the context of binding affinity for FcRn or binding of FcRn is to be interpreted in the context of any one or more of the above-mentioned assays for direct or indirect evaluation of affinity.
As defined herein, the FcRn binding dimer binds FcRn with a higher binding capacity compared to said first or second monomer unit alone. In one embodiment, the FcRn binding dimer may bind to FcRn with at least 2 times, such as at least 3 times, such as at least 4 times, such as at least 5 times, such as at least 6 times, such as at least 7 times, such as at least 8 times, such as at least 9 times, such as at least 10 times, such as at least 25 times, such as at least 50 times, such as at least 100 times higher capacity than the ponding first monomer unit or second monomer unit alone. This relationship may be true at both pH 6.0 and pH 7.4, or at pH 6.0 only, or at pH 7.4 only.
In someln some ments, explained further below, the FcRn binding dimer inhibits binding of lgG to FcRn. In such embodiments, said FcRn binding dimer may bind FcRn such that the ability of the FcRn binding dimer to block lgG binding to FcRn is at least 2 times , such as at least 3 times higher, such as at least 4 times higher, such as at least 5 times higher, such as at least 10 times, such as at least 15 times, such as at least times, such as at least 25 times higher compared to the blocking ability of the corresponding first or second monomer unit alone.
In some embodiments, said FcRn binding dimer may bind FcRn such that the MFI value of the interaction between FcRn and the FcRn binding dimer is at least 2 times higher, such as at least 3 times higher, such as at least 4 times higher, such as at least 5 times higher, such as at least 10 times higher compared to MFI value of the ction n FcRn and the corresponding first or second monomer unit alone.
In some embodiments, said FcRn binding dimer may bind FcRn such that the KB value of the ction between FcRn and the FcRn binding dimer is at least 2 times lower, such as at least 3 times lower, such as at least 4 times lower, such as at least 5 times lower, such as at least 10 times lower, such as at least 25 times lower, such as at least 50 times lower, such as at least 100 times lower, such as at least 1000 times lower compared to the KB W0 2016;042083 value of the interaction between FcRn and the corresponding first monomer unit or second r unit alone.
In one embodiment, there is provided an FcRn binding dimer, which is e of binding to FcRn at pH 6.0 such that the KB value of the interaction is at most 1 X 10'7 M, such as at most 1 X 10'8 M, such as at most 1 X 10'9 M, such as at most 1 X 10‘10 M, such as at most 1 X 10‘11 M, such as at most 1 X 10'12 M. An FcRn binding dimer according to this embodiment would bind, or remain bound, to FcRn in acidic pH conditions, such as pH 6.0, for example in an endosome. If such a polypeptide were to enter an increasingly acidic ellular environment, it would be recycled to the plasma membrane through its interaction with FcRn, and thus avoid degradation.
In one embodiment, the KB value of the interaction n an FcRn binding dimer and FcRn at pH 7.4 is higher than the KB value of said interaction at pH 6.0. Thus, the FcRn binding polypeptide would bind to FcRn with higher affinity at pH 6.0 than at pH 7.4. In one embodiment, the KB value of said interaction at pH 7.4 is at least 2 times higher, such as at least 5 times higher, such as at least 10 times higher, such at least 25 times, such as at least 50 times higher, such as at least 100 times, such as at least 1000 times higher than the KB value of said interaction at pH 6.0.
As mentioned above, FACS analysis may be used to e the interaction of between an FcRn binding dimer and FcRn. Hence, the interaction of between an FcRn binding dimer and FcRn at pH 6.0 and pH 7.4 can be evaluated and the MFI value at pH 6.0 and pH 7.4 may be compared as disclosed in the experimental section to follow. An obtained higher relative MFI value corresponds to a higher affinity and a lower relative MFI value corresponds to a lower affinity, provided that said MFI values are compared within the same eXperimental set up. Thus, in one ment, the FcRn binding dimer binds to FcRn with a higher affinity at pH 6.0 than at pH 7.4, such as at least 10 % higher, such as at least 20 % higher, such as at least 35 % higher, such as at least 50 % higher, such as least 100% higher.
In one embodiment, the KB value of the interaction between FcRn binding dimer and FcRn at pH 7.4 is at least 1 X 10'10 M, such as at least 1 x10'9 M, such as at least 1 x10'8 M, such as at least 1 X 10'7 M, such as at least 1 X 10'6 M, such as at least 1 X 10'5 M. In some embodiments, the only criterion for the interaction between FcRn binding dimer and FcRn at pH 7.4 is that any FcRn g dimer which has bound to FcRn during more acidic conditions is released more rapidly from FcRn when the pH value increases.
W0 2016;042083 2015/071339 In an alternative embodiment, there is ed an FcRn binding dimer, for which the KB of said ction at pH 7.4 is the same as or lower than the KB of said interaction at pH 6.0. An FcRn binding dimer according to this ment would bind or remain bound to FcRn in acidic pH conditions (i.e. would have an off-rate at pH 6.0 which is sufficiently slow to avoid release), for example in the endosome, as well as in neutral or slightly basic pH conditions, for example on the plasma membrane. In a more specific embodiment, the KB value of said interaction at pH 7.4 is at least 2 times lower, such as at least 5 times lower, such as at least 10 times lower, such as at least 50 times lower, such as at least 100 times lower than the KB value of said interaction at pH 6.0.
In another embodiment, there is provided an FcRn binding dimer, which is capable of binding to FcRn at pH 7.4 such that the KB value of the interaction is at most 1 x 10'7 M, such as at most 1 x 10'8 M, such as at most 1 x10'9 M, such as at most 1 X 10'10 M, such as at most 1 x 10'11 M, such as at most 1 x 10'12 M. An FcRn binding dimer according to this embodiment would bind or remain bound for an extended time to FcRn in neutral or slightly basic pH conditions, such as pH 7.4, for example on the plasma membrane.
The term “remain bound” should be understood to mean an interaction having a slow off-rate at given conditions.
In general, the skilled person knows that the KB value of an interaction is defined as the ratio between the off-rate (koff) and the on-rate (kon). Thus, a high KD value may be due to either a high koff, a low kon or both, and conversely, a low KD value may be due to either a low koff, a high kon or both.
The skilled person will understand that various modifications and/or additions can be made to an FcRn binding dimer according to any aspect disclosed herein in order to tailor the polypeptide to a specific ation without departing from the scope of the present disclosure.
For example, in one embodiment there is provided an FcRn binding dimer as described herein, wherein at least one of said first and second monomer units comprises at least one additional amino acid at the C-terminal and/or N-terminal end. Such a polypeptide should be tood as a polypeptide having one or more onal amino acid residues at the very first and/or the very last on in the polypeptide chain of at least one of said first and second monomer units. Thus, said at least one of said monomer units of the FcRn binding dimer as defined herein may comprise any suitable W0 2016;042083 number of additional amino acid residues, for example at least one additional amino acid residue. Each additional amino acid residue may individually or collectively be added in order to, for example, improve or simplify production, purification, stabilization in vivo or in vitro, ng, or detection of the polypeptide. Such onal amino acid residues may comprise one or more amino acid residues added for the purpose of chemical coupling. One example of this is the addition of a cysteine residue. Such additional amino acid residues may also provide a ”tag” for purification or detection of the polypeptide, such as a Hiss tag or a ”myc” (c—myc) tag or a ”FLAG” tag for interaction with antibodies specific to the tag or immobilized metal affinity chromatography (IMAC) in the case of the hexahistidine tag.
The further amino acids as discussed above may be coupled to the FcRn binding dimer or to any one or both of said first and second monomeric units by means of chemical conjugation (using known c chemistry methods) or by any other means, such as expression of the FcRn binding dimer as a fusion protein orjoined in any other fashion, either directly or via a linker, for example an amino acid linker as described above.
The further amino acids as sed above may for example comprise one or more ptide (s). A further ptide domain may e the FcRn g dimer with another on, such as for example another g function, or an enzymatic function, or a toxic function or a fluorescent signaling function, or combinations thereof.
Thus, in a second aspect of the present disclosure, there is provided a fusion protein or a conjugate, comprising a first moiety consisting of an FcRn binding dimer according to the first aspect, and a second moiety consisting of a polypeptide having a desired biological activity. In another embodiment, said fusion protein or conjugate may additionally comprise further moieties, comprising desired ical activities that can be either the same or different from the biological activity of the second moiety.
In one embodiment of said fusion protein or conjugate, the total size of the molecule is below the threshold for efficient renal clearance upon administration to a mammalian t.
In another embodiment of said fusion protein or conjugate, the total size of the molecule is above the threshold for efficient renal clearance upon administration to a mammalian subject.
W0 2016;042083 In one embodiment of said fusion protein or ate, the in vivo half- life of said fusion protein or conjugate is longer than the in vivo ife of the polypeptide having the desired biological activity per se.
Non-limiting examples of a desired ical activity comprise a therapeutic activity, a binding activity, and an enzymatic activity.
In one embodiment, said desired biological activity is a g activity to a selected target.
One example of such a binding ty is a binding activity, which increases the in vivo half-life of a fusion protein or conjugate. This fusion protein or conjugate may comprise at least one further moiety. In one particular ment, said target is albumin, binding to which increases the in vivo half-life of said fusion protein or conjugate. In one embodiment, said albumin binding activity is provided by an albumin binding domain (ABD) of streptococcal protein G or a derivative thereof. For example, said fusion protein or conjugate, comprising at least one further , may comprise [FcRn binding dimer] — [albumin binding moiety] — [moiety with affinity for selected ]. rmore, it will be appreciated that said fusion protein or conjugate may comprise an albumin g moiety or other target binding moiety interspaced between two FcRn binding monomer units making up the FcRn binding dimer as described herein, and may thus, as non—limiting examples, be arranged according to [FcRn binding monomer moiety] — in g moiety] — [FcRn binding monomer moiety] — [moiety with affinity for selected target] or according to [FcRn binding monomer moiety] — [moiety with affinity for selected ] — [FcRn binding monomer moiety] — [albumin binding moiety]. It is to be tood that the moieties in the fusion protein or conjugate may be freely arranged in any order from the N- to the C- terminal of the polypeptide. In one embodiment, said in vivo half-life is increased at least 10 times, such as at least 25 times, such as at least 50 times, such as at least 75 times, such as at least 100 times compared the in vivo half-life of the fusion protein or conjugate per se.
In one embodiment, when a complex between a target and the fusion protein or conjugate as described herein is formed (or maintained) at acidic pH, such as pH 6.0, the target is rescued from elimination by lysosomal degradation. Thus, target half-life is extended. Half-life extension implies that the elimination rate of a target is lower when interacting with said fusion protein or conjugate than the elimination rate of the target molecule in the absence of said fusion protein or conjugate. Furthermore, it is desirable in this W0 2016;042083 embodiment that the g of target by the fusion protein or conjugate should not interfere ntially with the function of the target.
On the other hand, when a complex between the target and the fusion protein or conjugate as described herein is not maintained or not formed at acidic pH, the target is directed to the subcellular lysosomes where it is degraded.
In one embodiment, there is provided a fusion protein or conjugate, wherein the rate of elimination of a selected, undesirable target from the subject is increased. Increased elimination of an undesirable target implies increased ation rate of the target from the body of the multicellular organism, as compared to a “normal” elimination rate of the target molecule per se, i.e. without previous ction with the fusion protein or conjugate.
In another embodiment, binding of a selected undesirable target could inactivate the function of the target, thereby blocking its biological activity in situations where this is desirable. Such biological activity may for example be tion or blocking of receptors or an enzymatic or othen/vise toxic or undesirable activity. Such undesirable target may be an endogenous hormone, enzyme, cytokine, chemokine or a target having some other ical activity. By using an inactivating target binding, the biological activity is blocked until the target is delivered for degradation and released at a low pH value, and the target binding fusion protein is recycled to circulation.
This ing of the target binding fusion protein (via its FcRn g ) enables it to “catalyze” the removal of more than one molecule of the ed undesirable .
Undesirable targets may for example be foreign proteins and compounds, or lly expressed proteins that display elevated levels in plasma following a medical condition and where a therapeutic effect may be achieved by ation of said protein. The undesired target is not necessarily evenly distributed in the plasma but may be concentrated in certain regions, for example around a tumor or at sites of ation.
Non-limiting examples of targets are targets selected from the group consisting of allergens, ds, antibodies, auto-antigens, blood clotting factors, hormones, tumor cells, drug molecules, cytokines, chemokines, proteases, hypersensitivity mediators, proinflammatory factors, toxins such as bacterial toxins and snake venoms; pollutants, metals and anti-oxidants.
Under certain conditions, such as in certain cancer es, it is desired to remove endogenous molecules, for example VEGF, PDGF, HGF W0 2016;042083 and other growth atory hormones. Such molecules could also be targeted by a binding function in said fusion protein or conjugate.
Under other conditions, such as in certain immunological diseases, it may be desirable to remove endogenous les transiently, such as selected interleukins or TNF. Such molecules could also be targeted by a binding function in said fusion protein or conjugate.
In one embodiment, the second moiety having a d biological activity is a therapeutically active polypeptide. Non-limiting es of eutically active polypeptides are biomolecules, such as molecules selected from the group consisting of s, for example algasidase d and B, glucocerebrosidase, dase, arylsulphatase, aglucosidase-d, ginase, Factor VII, Factor VIII, Factor IX and Factor Xa; hormones and growth factors, for example growth hormone, transforming growth factor-62, erythropoietin, insulin, insulin-like growth factor-1, myostatin, bone-derived growth factor and glucagon—like peptide-1; chemokines, for example CCL17, CCL19, CCL20, CCL21, CCL22, CCL27, XCL1 and CXCBCL1; and cytokines, for example interleukin (lL)-2, lL—4, lL-7, lL-10, lL-12, lL-15, lL-18, lL—22, lL-27, interferon (lFN)—d, lFN-B, lFN-y, tumor necrosis factor (TNF), granulocyte-colony stimulating factor (G-CSF), macrophage-CSF, and granulocyte/macrophage-CSF.
As the skilled person understands, the FcRn binding dimer according to the first aspect may be useful in a fusion protein or as a conjugate partner to any other moiety. Therefore, the above lists of therapeutically active polypeptides should not be ued as limiting in any way.
Other possibilities for the creation of fusion polypeptides or conjugates are also contemplated. Thus, an FcRn binding dimer according to the first aspect of the invention may be covalently d to a second or further moiety or moieties, which, in addition to or instead of target binding, exhibit other functions. One example is a fusion between one or more FcRn binding dimer and an enzymatically active polypeptide serving as a reporter or effector moiety.
With regard to the description above of fusion proteins or conjugates incorporating an FcRn g dimer according to the disclosure, it is to be noted that the designation of first, second and further moieties is made for clarity reasons to distinguish between FcRn binding dimer according to the W0 2016;042083 disclosure on the one hand, and moieties exhibiting other functions on the other hand. These designations are not intended to refer to the actual order of the different domains in the polypeptide chain of the fusion protein or conjugate. Thus, for example, said first moiety may without restriction appear at the N-terminal end, in the middle, or at the C-terminal end of the fusion protein or conjugate. Furthermore, the FcRn binding dimer as disclosed herein may comprise a second moiety interspaced between the two FcRn binding monomer units of the FcRn binding dimer.
The half maximal inhibitory concentration (IC50) is a measure of the effectiveness of a substance for inhibiting a specific quantifiable biological or biochemical function. This quantitative measure indicates how much of a particular substance is needed to inhibit a specific ical function by 50 % and is commonly used in the art. In one particular embodiment, there is provided an FcRn binding dimer, fusion n or conjugate as defined herein capable of blocking lgG binding to FcRn such that the half maximal inhibitory concentration (IC50) of the blocking is at most 1 x 10'8 M, such as at most 6 x '9 M, such as at most 4 x 10'9 M, such as at most 1 x10'9 M, such as at most 1 x10'10 M, such as at most 1 x10'11 M. In one embodiment, there is provided an FcRn g dimer, fusion protein or conjugate as defined herein capable of ng lgG binding to FcRn such that the half maximal inhibitory tration (IC50) of the blocking is at least 10 times lower, such as at least 100 times lower, such as at least 1000 times lower compared to the |C50 of the blocking by the corresponding first or second monomer unit alone.
The tion may be due to binding of the FcRn binding dimer, fusion protein or ate to the same, or an at least partially pping, region of FcRn as lgG. Alternatively, the FcRn binding dimer, fusion protein or conjugate may bind to a different region of FcRn than lgG but sterically hinder the binding of lgG to FcRn. Thus, the rate of ation or clearance of lgG from the circulatory system would increase due to increased lysosomal degradation of lgG, because the FcRn mediated recycling of lgG would be wholly or partially unavailable due to the occupation of FcRn binding sites by the FcRn g dimer according to the present disclosure. In other words, administration of FcRn binding dimer, fusion n or conjugate according to the present disclosure will act to increase the lism of circulating lgG antibodies.
W0 2016;042083 In one embodiment, the KB value of the ction between the FcRn binding dimer, fusion protein or ate and FcRn is lower than the KB of the interaction between lgG and FcRn. This relationship may be true at both pH 6.0 and pH 7.4, or at pH 6.0 only.
The above aspects furthermore encompass polypeptides in which the FcRn binding dimer according to the first aspect, or the FcRn binding dimer as comprised in a fusion protein or conjugate according to the second aspect, further comprises a label, such as a label selected from the group consisting of fluorescent dyes and metals, chromophoric dyes, chemiluminescent compounds and bioluminescent proteins, enzymes, uclides and radioactive particles. Such labels may for example be used for detection of the polypeptide.
In other embodiments, the labeled FcRn binding dimer is present as a moiety in a fusion protein or conjugate also comprising a second moiety having a desired biological activity and/or comprising a binding on as described above. The label may in some instances be coupled only to the FcRn binding dimer (for example to one, two or both of said first and second monomeric units), and in some instances both to the FcRn binding dimer and to the second moiety of the conjugate or fusion protein. rmore, it is also possible that the label may be coupled to a second moiety only and not to the FcRn binding moiety. Hence, in yet another embodiment there is provided an FcRn binding dimer comprising a second moiety, wherein said label is coupled to the second moiety only.
When reference is made to a labeled ptide, this should be understood as a reference to all s of the FcRn g dimer as described herein, including fusion proteins and conjugates comprising an FcRn binding dimer and a second and optionally further es. Thus, a labeled polypeptide may contain only the FcRn binding dimer and e.g. a therapeutic radionuclide, which may be chelated or covalently coupled to the FcRn binding dimer, or contain the FcRn binding dimer, a therapeutic radionuclide and a second moiety such as a small molecule having a desired biological activity, for e resulting in a therapeutic efficacy. ln embodiments where the FcRn binding dimer, fusion protein or conjugate is radiolabeled, such a radiolabeled polypeptide may comprise a radionuclide. A ty of radionuclides have a metallic nature, are used in the ionic form, and are typically incapable of forming stable covalent bonds W0 2016;042083 with elements presented in proteins and peptides. For this reason, labeling of proteins and peptides with radioactive metals is performed with the use of ors, i.e. multidentate ligands, which form non—covalent compounds, called chelates, with the metal ions. In an embodiment of the FcRn binding dimer, fusion protein or conjugate, the incorporation of a radionuclide is enabled through the provision of a chelating environment, through which the radionuclide may be nated, chelated or complexed to the polypeptide.
One example of a chelator is the inopolycarboxylate type of chelator. Two classes of such polyaminopolycarboxylate chelators can be distinguished: macrocyclic and acyclic chelators.
In one ment, the FcRn binding dimer, fusion protein or conjugate comprises a ing environment provided by a polyaminopolycarboxylate chelator coupled to the FcRn binding dimer via a thiol group of a cysteine residue or an epsilon amine group of a lysine residue. Alternatively, the polyaminopolycarboxylate or may be coupled to any part of the fusion protein or ate as disclosed herein, such as to the second or further moiety of said fusion protein or conjugate.
The most ly used macrocyclic chelators for sotopes of indium, gallium, yttrium, bismuth, radioactinides and radiolanthanides are different derivatives of DOTA (1 ,4,7,10-tetraazacyclododecane-1 ,4,7,10- tetraacetic acid). In one embodiment, a chelating environment of the FcRn binding dimer, fusion protein or ate is provided by DOTA or a derivative thereof. More specifically, in one ment, the chelating polypeptides encompassed by the t disclosure are obtained by reacting the DOTA derivative 1,4,7,10-tetraazacyclododecane-1,4,7-tris-acetic acid maleimidoethylacetamide (maleimidomonoamide-DOTA) with said polypeptide.
Additionally, 1,4,7-triazacyclononane-1,4,7-triacetic acid (NOTA) and derivatives thereof may be used as chelators. Hence, in one embodiment, there is provided an FcRn binding dimer, fusion protein or conjugate, wherein the polyaminopolycarboxylate chelator is 1,4,7-triazacyclononane-1,4,7- triacetic acid or a derivative thereof.
The most ly used acyclic polyaminopolycarboxylate chelators are different derivatives of DTPA (diethylenetriamine-pentaacetic acid).
Hence, polypeptides having a chelating environment provided by diethylenetriaminepentaacetic acid or derivatives thereof are also encompassed by the t disclosure.
W0 2016;042083 In a further embodiment, the FcRn binding dimer, produced recombinantly h expression of a polynucleotide or synthetically, is conjugated to one or more synthetic polymers, in order for example to increase its hydrodynamic radius. Polyethylene glycol (PEG) is ly used for this purpose, but other polymers have also been used in the art.
Such “PEGylation” may be used to increase the size of the FcRn binding dimer, fusion protein or conjugate as described herein to a size above the threshold for effective renal ion.
In one embodiment, a synthetic polymer is conjugated to one or more chemically sized FcRn binding dimer(s). Other functionalities may also be conjugated to the same synthetic r. If the FcRn binding dimer and other components are chemically synthesized, none of the components will have to be made in a biological system if this is not desired.
In a preferred embodiment, one or more synthetically or biologically manufactured FcRn binding dimers are conjugated to a tic r, to e a size exceeding the size associated with efficient renal clearance and used for blocking binding of lgG to FcRn. A unique cysteine in the monomer units of the FcRn binding dimer may be used for site specific conjugation, for example a C-terminally located ne introduced for this purpose. With a branched tic polymer, more than two FcRn binding moieties may be conjugated to the same polymer, to enhance the avidity and therefore the blocking potency.
In a third aspect of the present disclosure, there is provided a polynucleotide encoding an FcRn binding dimer or a fusion protein as described herein. Also encompassed by this disclosure is a method of producing an FcRn binding dimer or fusion protein as described above comprising expressing the polynucleotide; an expression vector comprising the cleotide; and a host cell comprising the expression vector.
Also encompassed is a method of ing FcRn binding dimer or a fusion protein, comprising culturing said host cell under conditions permissive of expression of said polypeptide from its expression vector, and isolating the polypeptide.
The FcRn binding dimer or fusion protein of the present disclosure may alternatively be produced by non-biological peptide synthesis using amino W0 2016;042083 acids and/or amino acid derivatives having protected reactive hains, the non-biological peptide synthesis comprising - step-wise coupling of the amino acids and/or the amino acid derivatives to form an FcRn binding dimer or a fusion protein having protected reactive side-chains, - removal of the protecting groups from the reactive side-chains of the FcRn binding dimer or fusion protein, and - folding of the FcRn binding dimer orfusion n in aqueous solution.
In a fourth aspect of the disclosure, there is provided a composition comprising an FcRn binding dimer, fusion protein or conjugate as described herein and at least one pharmaceutically acceptable ent or carrier. In one embodiment thereof, said composition r comprises at least one additional active agent, such as at least two additional active agents, such as at least three additional active agents. Non-limiting es of additional active agents that may prove useful in such a ation are immunosuppressing agents, anti-inflammatory agents, icrobial agents and enzymes.
In one embodiment of this aspect, said composition is adapted for administration by a route selected from the group consisting of oral administration, intranasal administration, pulmonar administration, vaginal stration, rectal administration, intravenous ion, intraperitoneal injection, intramuscular ion, subcutaneous injection and intradermal injection.
As used herein, the term “systemic administration” refers to a route of administration such that the substance of interest enters into the circulatory system so that the entire body is affected. The skilled person is aware that ic administration can take place via enteral administration (absorption of the drug through the gastrointestinal tract) or parenteral administration ally injection, infusion or implantation).
In one embodiment, said composition is adapted for administration systemically or locally. In certain embodiments, systemic administration of said composition may be used. In another ment, said composition is adapted for administration by a local route. For example, local administration may be topical in an ointment, paste, foam or cream. In another embodiment, W0 42083 said composition is adapted for administration across an endothelial or lial layer. Here, the composition may be transcytosed across said layer.
In one embodiment, the rate of uptake of a composition comprising a fusion protein or conjugate as described herein is higher than the rate of uptake of polypeptides corresponding to second or further moieties per se. In one embodiment, the rate of uptake is at least 2 times higher, such as at least times , such as at least 10 times , such as at least 25 times higher than the rate of uptake of the at second or further moieties per se.
It should be understood from the above disclosure that the FcRn binding dimer, fusion protein or conjugate or the composition as described herein may for example be useful as a therapeutic agent, and/or as a means for ing the in vivo half-life of a fusion partner, and/or as a means for increasing the rate of elimination of undesirable targets.
Hence, in a fifth aspect of the present disclosure, there is provided an FcRn binding dimer, fusion protein, conjugate or composition as disclosed herein for use as a medicament.
In a related, sixth, aspect of the present disclosure, there is provided a method of treatment or prophylaxis of a subject in need thereof, comprising the step of administrating a therapeutically or prophylactically active amount of an FcRn binding dimer, fusion protein, conjugate or composition as disclosed herein.
In one embodiment of any one of these two latter aspects, the medicament or method is intended for reduction of an IgG level in a subject in need thereof.
In one embodiment of any one of these two latter aspects, the medicament or method is intended for treatment or prophylaxis in which the capacity of the FcRn g dimer to at least partially block g of IgG to FcRn is exploited, for e treatment or laxis in which increased catabolism of IgG antibodies is desired.
In another embodiment wherein the IgG blocking capacity is used, the administration of FcRn binding dimers as described herein has the effect of improving the efficacy of a drug, by blocking antibodies that exhibit anti-drug properties. In particular, the action of drugs that are cleared by antibodies or for which lizing antibodies are induced may be improved in this way, by administration of FcRn binding dimers prior to administration of the drug in question.
W0 2016;042083 In another embodiment wherein the lgG blocking capacity is used, the administration of FcRn binding dimers as described herein has the effect of reducing harmful effects of antibodies by ng them or reducing their circulation time in the bloodstream of a subject. For example, radiolabelled or toxin-conjugated dies may be removed by the subsequent administration of FcRn binding dimers as disclosed herein. atively, in cases where toxic adverse effects occur as a reaction against a eutic antibody drug, such adverse effect may be ameliorated or neutralized by the subsequent administration of an FcRn g dimer to remove such antibodies or limit their circulation time.
In one embodiment, a condition in which such treatment or prophylaxis may be indicated is an auto-immune condition. As non-limiting examples of indicated conditions, mention is made of acute disseminated encephalomyelitis (ADEM), acute necrotizing hemorrhagic leukoencephalitis, Addison’s e, globulinemia, alopecia , amyloidosis, ANCA-associated vasculitis, ankylosing litis, anti-GBM/anti-TBM nephritis, antiphospholipid syndrome (APS), autoimmune angioedema, autoimmune aplastic anemia, mune dysautonomia, autoimmune hepatitis, autoimmune hyperlipidemia, mune immunodeficiency, autoimmune inner ear disease , autoimmune limbic encephalitis, autoimmune myocarditis, autoimmune pancreatitis, autoimmune retinopathy, autoimmune thrombocytopenic purpura (ATP), autoimmune thyroid e, autoimmune urticarial, axonal & anal neuropathies, Balo disease, Behcet’s e, bullous pemphigoid, cardiomyopathy, man disease, celiac disease, Chagas disease, chronic inflammatory demyelinating polyneuropathy (CIDP), chronic recurrent ocal ostomyelitis (CRMO), Churg-Strauss syndrome, cicatricial pemphigoid/benign mucosal pemphigoid, Crohn’s disease, Cogans syndrome, cold agglutinin disease, congenital heart block, coxsackie myocarditis, CREST disease, essential mixed cryoglobulinemia, demyelinating neuropathies, dermatitis herpetiformis, dermatomyositis, s disease (neuromyelitis optica), dilated cardiomyopathy, discoid lupus, Dressler’s syndrome, endometriosis, eosinophilic angiocentric fibrosis, eosinophilic fasciitis, epidermolysis bullosa acquisita, erythema m, experimental allergic encephalomyelitis, Evans syndrome, fibrosing alveolitis, giant cell arteritis (temporal arteritis), glomerulonephritis, Goodpasture’s syndrome, granulomatosis with polyangiitis (GPA; Wegener’s), Graves’ disease, Guillain-Barré syndrome, W0 2016;042083 Hashimoto’s encephalitis, Hashimoto’s thyroiditis, hemolytic anemia, Henoch- Schonlein purpura, herpes gestationis, hypogammaglobulinemia, idiopathic hypocomplementemic tubulointestitial nephritis, idiopathic membranous nephropathy, idiopathic thrombocytopenic purpura (ITP), lgA pathy, lgG4-related disease, lgG4-related sclerosing disease, regulatory Iipoproteins, matory aortic aneurysm, inflammatory pseudotumor, inclusion body myositis, insulin-dependent diabetes (type 1), interstitial cystitis, juvenile tis, juvenile diabetes, Kawasaki syndrome, Kuttner’s tumor, Lambert-Eaton syndrome, leukocytoclastic vasculitis, lichen planus, lichen sclerosus, Iigneous conjunctivitis, linear lgA disease (LAD), lyme disease, chronic mediastinal fibrosis, Meniere’s disease, microscopic polyangiitis, Mikulicz’s syndrome, mixed connective tissue disease (MCTD), Mooren’s ulcer, morvan syndrome, Habermann disease, mucus membrane pemphigoid, multifocal fibrosclerosis, multiple sis, myasthenia , myositis, narcolepsy, neuromyelitis optica (Devic’s), yotonia ’s syndrome), neutropenia, ocular cicatricial pemphigoid, optic neuritis, Ormond’s disease (retroperitoneal is), palindromic rheumatism, PANDAS (pediatric autoimmune neuropsychiatric disorders associated with streptococcus), paraneoplastic cerebellar degeneration, paraproteinemic polyneuropathies, smal nocturnal hemoglobinuria (PNH), Parry g syndrome, Parsonnage-Turner syndrome, pars planitis (peripheral uveitis), pemphigoid gestationis, pemphigus vulgaris, periaortitis, periarteritis, peripheral neuropathy, perivenous encephalomyelitis, pernicious anemia, POEMS syndrome, polyarteritis nodosa, thritis, Type I, ll, & lll autoimmune polyglandular syndromes, polymyalgia rheumatic, polymyositis, postmyocardial infarction syndrome, ricardiotomy me, progesterone dermatitis, primary biliary cirrhosis, primary sclerosing cholangitis, psoriasis, psoriatic arthritis, thic pulmonary fibrosis, pyoderma gangrenosum, pure red cell aplasia, Raynaud’s phenomenon, reflex sympathetic dystrophy, Reiter’s syndrome, ing polychondritis, restless legs syndrome, retroperitoneal fibrosis (Ormond’s disease), rheumatic fever, rheumatoid arthritis, Riedel’s thyroiditis, sarcoidosis, Schmidt me, scleritis, scleroderma, n’s syndrome, sperm & testicular autoimmunity, stiff person me, subacute bacterial endocarditis (SBE), Susac’s syndrome, sympathetic ophthalmia, Takayasu’s arteritis, systemic lupus erythematosus (SLE), temporal arteritis/giant cell arteritis, thrombotic thrombocytopenic purpura (TTP), -Hunt syndrome, W0 2016;042083 erse myelitis, ulcerative colitis, undifferentiated tive tissue disease (UCTD), s, vasculitis, vesiculobullous osis, vitiligo, Waldenstrom macroglobulinaemia and warm thic hemolytic anemia.
In another embodiment of the fifth and sixth aspects, a condition in which such treatment or prophylaxis may be ted is an allo-immune condition. As non-limiting examples of indicated conditions, mention is made of transplantation donor mismatch due to anti-HLA antibodies; foetal and neonatal alloimmune thrombocytopenia, FNAIT (or neonatal alloimmune thrombocytopenia, NAITP or NAIT or NAT, or foeto—maternal alloimmune thrombocytopenia, FMAITP or FMAIT).
In another embodiment of the fifth and sixth s, a condition in which such treatment or prophylaxis may be indicated is selected from the group consisting of autoimmune polyendocrine syndrome types 1 (APECED or Whitaker’s Syndrome) and 2 (Schmidt’s Syndrome); alopecia salis; myasthenic crisis; thyroid crisis; thyroid associated eye disease; thyroid ophthalmopathy; autoimmune diabetes; autoantibody associated encephalitis and/or encephalopathy; pemphigus foliaceus; epidermolysis bullosa; dermatitis herpetiformis; Sydenham’s chorea; acute motor axonal neuropathy (AMAN); Miller-Fisher me; multifocal motor neuropathy (MMN); onus; inflammatory myopathy; Isaac’s syndrome (autoimmune neuromyotonia), oplastic syndromes and limbic encephalitis.
In another embodiment of the fifth and sixth aspects, a ion in which such treatment or prophylaxis may be indicated is selected from epilepsy and seizures.
In another embodiment, there is provided an FcRn binding dimer, fusion n, conjugate or composition as described herein for use in blocking or removal of an undesirable target from the circulation. In one embodiment, said undesirable target is selected from the group comprising allergens, amyloids, antibodies, auto-antigens, blood clotting factors, hormones, tumor cells, drug les, cytokines, chemokines, hypersensitivity mediators, pro-inflammatory factors, toxins such as bacterial toxins and snake venoms, pollutants, metals and anti-oxidants.
While the invention has been bed with reference to various ary aspects and embodiments, it will be understood by those skilled in the art that various changes may be made and equivalents may be substituted for elements f without departing from the scope of the invention. In addition, many modifications may be made to adapt a particular situation or molecule to the ngs of the invention without departing from the essential scope thereof. ore, it is intended that the invention not be limited to any particular embodiment contemplated, but that the invention will include all embodiments falling within the scope of the appended claims.
Brief description of the figures Figure 1 is a listing of the amino acid sequences of examples of FcRn binding polypeptides in monomeric form (SEQ ID NO:1-367) and FcRn binding ptides in dimeric form (SEQ ID NO:368—376), as well as the amino acid sequences of the albumin binding polypeptide variant PP013 (SEQ ID NO:377), Taq polymerase binding Z variant 203638 (SEQ ID NO:378), human chRn (SEQ ID NO:379), murine chRn (SEQ ID NO:384), human BZ-microglobulin (SEQ ID ), murine BZ-microglobulin (SEQ ID NO:381), human chRn (SEQ ID NO:382) when in human FcRn-eGFP and murine chRn (SEQ ID NO:383) when in murine FcRn-eGFP. The deduced FcRn binding motifs (BMs) of the FcRn g polypeptides disclosed herein extend from e 8 to residue 36 in sequences with SEQ ID NO:1-367.
The amino acid sequences of the 49 amino acid residues long polypeptides (BMod) predicted to constitute the complete three-helix bundle within each of these Z ts extend from residue 7 to residue 55.
Figures 2A-2E show the binding to human FcRn at pH 6.0 and dissociations at pH 6.0 and 7.4 for agged Z variants and for lgG as described in Example 3. Overlays of grams obtained from a Biacore instrument representing injection at pH 6.0 followed by dissociation at pH 6.0 (solid line) and injection at pH 6.0 followed by dissociation at pH 7.4 (dashed line) are yed for (A) 207918 (SEQ ID NO:1), (B) ZO7960 (SEQ ID NO:4), (C) Z10109 (SEQ ID NO:3), (D) Z10193 (SEQ ID N02) and (E) lgG.
Figure 3 shows dot plots from a flow cytometry analysis of binding of FcRn binding Z variant to human (upper panel) and mouse (lower panel) FcRn-eGFP HeLa cells, as described in Example 4. Due to heterogeneous expression of FcRn-eGFP by HeLa cells, cells were gated according to FcRn- eGFP expression level. Cells in gate H are considered to be FcRn—eGFP negative and cells in gate l are considered to be positive. Incubation with Alexa Fluor® 647 labeled Z variants ed in a population positive both for Alexa Fluor® 647 and eGFP, whereas incubation with buffer (buffer control) did not. The figure shows that the three variants 207960 (SEQ ID NO:4), 207930 (SEQ ID NO:6) and 207918 (SEQ ID NO:1) bind to human FcRn and mouse FcRn. The y-axis shows Alexa Fluor® 647 intensity and the x-axis shows eGFP activity.
Figure 4 shows mean scence intensity (MFI) values of Alexa Fluor® 647 labeled 207960 (SEQ ID NO:4), 207930 (SEQ ID N026) and 207918 (SEQ ID NO:1), measured in the cell binding assay described in Example 4. Diagram (A) shows MFI from HeLa cells transduced with human FcRn-eGFP and diagram (B) shows MFI from HeLa cells transduced with mouse FcRn-eGFP.
Figure 5 shows dot plots from flow cytometry is of human or mouse lgG Alexa Fluor® 647 g to human (upper panel) and mouse (lower panel) FcRn-eGFP HeLa cells, as described in Example 5. Due to heterogeneous expression of FcRn—eGFP by HeLa cells, cells were gated ing to the abundance of FcRn-eGFP on the cell surface. Cells in gate M are considered to be FcRn—eGFP negative and cells in gate N are considered to be positive. Binding of 100 nM human or mouse lgG-Alexa Fluor® 647 to FcRn transduced HeLa cells are shown in the left panel (0 nM).
The figure shows that lgG binding was blocked by Hiss-tagged 207918 (SEQ ID NO:1) in a dose dependent manner (1, 10, 100 and 1000 nM). The y-axis shows Alexa Fluor® 647 intensity and the x-axis shows eGFP activity.
Figure 6 shows mean scence intensity (MFI) values resulting from FcRn binding of IgG Alexa Fluor® 647 in the presence of different concentrations of Hise-tagged 207918 (SEQ ID NO:1) on (A) human FcRn- eGFP transduced HeLa cells and (B) mouse FcRn-eGFP transduced HeLa cells, as described in Example 5. The figure shows dose dependent blocking of the lgG-FcRn binding by the 2 variant.
Figures 7A-7C show kinetics of binding of three 2 variants to human FcRn at pH 6.0, as described in e 6, using a Biacore instrument.
Sensorgrams for a tration series of (A) 211948 (SEQ ID ), (B) 211946 (SEQ ID NO:355) and (C) 211947 (SEQ ID NO:356), respectively, in fusion with the albumin binding polypeptide PP013 (SEQ ID NO:377) and the control 2 variant molecule 203638 (SEQ ID NO:378; not specific for FcRn), are displayed. Curves from 640 nM (dashed line), 160 nM (dotted line) and 40 nM (solid grey line) were subjected to kinetic analysis using the Langmuir 1:1 binding model. Kinetic parameters and ties were calculated from fitted curves (solid black lines) and are shown in Table 6.
Figure 8 shows the pharmacokinetic profiles for three FcRn binding Z variants fused to the albumin g polypeptide PP013 obtained as described in Example 6. The Z ts Z11947 (SEQ ID NO:356, open squares), 211946 (SEQ ID NO:355, open les) and 211948 (SEQ ID NO:354, open diamonds) all displayed prolonged ife compared to the negative control ZO3638-PP013 (open circles).
Figure 9 shows the blocking of human IgG to human FcRn by His;- ZO7918 (SEQ ID NO:1; black circles), IVIg (grey squares) and SCIg (grey triangles), respectively, assayed as described in Example 10.
Figure 10 shows that blocking of the IgG-FcRn interactions with FcRn specific Z variants in mice results in reduced levels of IgG. As further described in Example 11, mice were treated with five daily injections of Vehicle (+), the ABD fused Z variant ZO7918-PP013 (open square) and 211948 (SEQ ID NO:354; closed circle). The concentration of endogenous IgG was measured by ELISA. The tration of IgG in individual mice at 24, 72, 120 and 168 h was related to the level at 0 h and the results are therefore presented as percentage of IgG at 0 h.
Figure 11 shows mean fluorescence intensity (MFI) values of Alexa Fluor® 647 labeled dimeric and ric polypeptides binding to human FcRn-eGFP transfected HeLa cells measured as described in Example 13.
(A) Dimers Z11948-(G4S)3-Z11948 (SEQ ID NO:369) and Z11948-(G4S)— Z11948 (SEQ ID NO:368), and a corresponding monomer Z variant, ZO7918 (SEQ ID NO:1), g to FcRn at pH 6 (black) and pH 7.4 (white). (B) Monomer primary Z variant ZO7918 (SEQ ID NO:1) and monomer maturated Z variants Z13583 (SEQ ID NO:23), Z13621 (SEQ ID NO:44), Z13654 (SEQ ID NO:65) and 213674 (SEQ ID NO:75), binding to FcRn at pH 6 (black) and pH 7.4 (white).
Figure 12 shows the blocking of human IgG binding to human FcRn by dimeric and monomeric polypeptides assayed as described in Example 14.
(A) Dimers Z11948-(G4S)3-Z11948 (SEQ ID NO:369) and Z11948-(G4S)- Z11948 (SEQ ID NO:368); a corresponding monomer Z variant, ZO7918 (SEQ ID NO:1), SCIg and Mg. B) Monomer primary Z variant ZO7918 (SEQ ID NO:1) and r maturated Z variants Z13583 (SEQ ID N023) and 213621 (SEQ ID NO:44).
Figure 13 shows pH dependent binding of ptides to hFcRn ed by ELISA as described in Example 17. (A) Binding of the indicated polypeptides at pH 6. (B) g of the indicated polypeptides at pH 7.4. At W0 2016;042083 both pH , more efficient binding was seen for the dimeric polypeptides (ZAZ####) than for the monomeric Z variant (Z13621).
Figure 14 shows reduction of hlgG levels in FcRn transgenic mice treated with dimeric polypeptides as described in Example 20. (A) Reduction of hlgG levels was equally efficient with the albumin binding domain PP013 (SEQ ID ) situated between the two 2 moieties (ZAZ3715; SEQ ID NO:371) as with PP013 situated at the C-terminus of the polypeptide (ZZA3716; SEQ ID NO:372). (B) Equal ion of hlgG levels was obtained with the polypeptides ZAZ3869 (SEQ ID NO:374), ZAZ3870 (SEQ ID NO:375) and ZAZ3871 (SEQ ID NO:376).
Figure 15 shows dose dependent reduction of hlgG levels in NMRI mice d with dimeric polypeptides ZAZ3715 (SEQ ID NO:371) and ZAZ3824 (SEQ ID NO:373) as described in Example 21.
Figure 16 shows the serum concentrations of ZAZ3715 (SEQ ID NO:371) and ZAZ3824 (SEQ ID NO:373), respectively, measured in the same lgG catabolism study as that presented in Figure 15.
Figure 17 is an image of an SDS-PAGE gel showing al and d FcRn binding Z variants before (0) and after a 2 week (2w) stability test. Lane 1: Z11948 (0), lane 2: Z11948 (2w), lane 3: Mw, lane 4: Z17347 (0), lane 5: 217347 (2w), lane 6: Z17348 (0), lane 7: 217348 (2w). The molecular size marker (Mw) was Novex® Sharp Pre-stained Protein Standard (216, 160, 110, 80, 60, 50, 40, 30, 20, 15, 10, 3.5 kDa). The diagonal bands seen in the figure are an artifact resulting from an t from a second gel stained in the same ner.
Figure 18 shows the binding to human and cynomolgus FcRn at pH 6.0 as described in Example 25. Overlays of sensorgrams ed from a Biacore instrument representing responses from injection of 90 nM Hise- tagged Z variant over hFcRn ) and chRn (grey) are displayed for (A) Z13578 (solid line) and 218632 (dashed line), (B) Z13616 (solid line) and Z18633 (dashed line), and (C) Z13621 (solid line) and Z18634 (dashed line).
Examples Summary The following Examples disclose the development of novel Z variant molecules targeting the al Fc receptor (FcRn). The Z variants were obtained using phage display technology. The genes encoding FcRn g polypeptides described herein were sequenced, and the corresponding amino acid sequences are listed in Figure 1, and denoted by the fiers SEQ ID NO:1-353. The deduced FcRn binding motifs (BMs) of the FcRn binding polypeptides disclosed herein extend from residue 8 to residue 36 in sequences with SEQ ID NO:1—353. Furthermore, the FcRn binding properties and ability to block lgG binding to FcRn of said polypeptides in dimeric form were investigated.
Example 1 tion of human chRn and human roglobulin (82M) In this Example, the extracellular domain (ECD) of human chRn (SEQ ID ) in complex with human BZ-microglobulin (SEQ ID NO:380) (complex denoted FcRn) and human BZ-microglobulin in non-complexed form (denoted BZM) were produced as soluble ns. Human FcRn and BZM produced in this Example were used for phage selection, ELISA and Biacore assays in Examples 2 and 3.
Materials and methods Construction of plasmids containing the genes for human chRn and human fi2-microglobulin to be used for co-expression: The genes encoding human chRn (Genbank BC008734.2) and human BZ-microglobulin (82M) (Genbank 89.1) were obtained from OpenBiosystems. Using PCR overlap extension, a gene fragment encoding amino acids 24-290 of human chRn (chRnECD) (SEQ ID ) was amplified to a construct ting of attB1-site/Kozak sequence followed by a gene encoding: an lg kappa chain leader sequence, hFcRnECD, a GS-linker and a flag tag, followed by an attBZ site. A similar construct was made containing a gene fragment encoding amino acids 21-119 of human 82M (SEQ ID NO:380), except that a Hise tag replaced the flag tag. The constructs were inserted into the plasmid pDONOR221 rogen, cat. no. 12536-017) by recombination using the Gateway system (Invitrogen, cat. no. 11789020, y® BP Clonase® II Enzyme mix), according to the manufacturer’s recommendations. After verification of correct sequences, the human chRnECD construct was inserted into 2K7bsd (Suter et al. (2006) Stem Cells 24:615—623) using multi- site gateway cloning together with the promoter-containing plasmid pENTR- CMV (Tai et al. (2012) PLoS One 7(9):e46269), resulting in the vector 2K7bsd- CMV-hFcRnECD. The human 82M gene uct was similarly inserted into 2K7neo (Suter et al., supra), giving the vector 2K7neo—CMV-h 82M.
Cell culture, preparation of recombinant lentiviral vectors and gene insertions into SKOV-3 cell line: The HEK293T and SKOV—3 cell lines were obtained from ATCC. Cells were grown at 37 °C in a humidified incubator in the presence of 5 % C02. Complete medium for the HEK293T cell line was Dulbeccos modified eagle medium (DMEM) supplemented with 10 % fetal bovine serum (FBS), 1 % Antibiotic Antimycotic Solution (AA) and 1 % MEM sential Amino Acid Solution (NEAA). Complete medium for the SKOV- 3 cell line was McCoy’s 5A medium mented with 10 % FBS and 1 % The plasmids 2K7bsd-CMV-hFcRnECD and 2K7neo-CMV-hBZM were separately co-transfected together with VSV-G envelope and gag/pol packaging plasmid into HEK293T cells using calcium chloride transfection (Zufferey etal. (1997) Nat Biotechnol 15(9):871-5; Jakobsson etal. (2006) J Neurosci Res 84:58-67). HEK293 culture atants containing formed lentiviral particles with human chRnECD and human BZM transgenes, respectively, were cleared from cell debris by centrifugation and filtration. The two types of lentiviral particles were used to sequentially transduce SKOV-3 cells. Successful double integrants ning both the human chRnECD and the BZM genes were selected for by the addition of blasticidin (lnvitrogen) and G418 sulfate (lnvitrogen) to culture medium while passaging the cells for two weeks. The resulting, stably transduced SKOV-3 cell line was denoted SKOV— 3 hFcRnECD/hBZM. sion of recombinant human FcRn: SKOV-3 cells, ressing human chRnECD and BZM resulting in human FcRn, were ed and 1.5 x 107 cells were seeded in a HYPERFlask (Coming) in 560 ml complete growth medium. After five days, when the cells had settled and multiplied, the medium was d to complete growth medium without FBS. After five days, the e was terminated and the supernatant was ted, passed through a 45 pm filter and frozen at -80 °C.
Purification of recombinant human FcRn using human lgG chromatography: Protein purification was carried out in an AKTA Explorer system (GE Healthcare). Human lgG (Pharmacia), 1 ml in 0.2 M NaHC03, 0.5 M NaCl pH 8.3 at a concentration of 10 mg/ml, was coupled to a 1 ml HiTrap NHS-activated HP column (GE Healthcare) according to the manufacturer’s W0 2016;042083 instruction. The supernatant containing inant human FcRn from SKOV-3 cells was thawed and the pH was ed to 5.8 with HCI. The supernatant was subsequently loaded in batches of 100 ml onto the column previously equilibrated with 20 mM Bis-Tris pH 5.8. The column was washed with 20 ml of 20 mM Bis-Tris pH 5.8 and eluted in fractions of 1 ml using 50 mM Tris, pH 8.1. Buffer exchange to PBS (phosphate buffered saline, 10 mM phosphate, 137 mM NaCl, 2.68 mM KCI, pH 7.4) was performed using dialysis.
GE and Western blot: The purity of the eluted fractions from the protein purification was analyzed by SDS-PAGE and staining with e Blue Stain Reagent (Pierce) and SilverXpress® Silver Staining Kit (lnvitrogen). Western blotting was carried out using an Amersham HybondTM- C Extra nitrocellulose membrane (GE Healthcare). The membrane was blocked with 5 % non—fat dry milk (Semper) in TBS+T (50 mM Trizma base, 150 mM NaCl, 0.05 % Tween-20, pH 8) for 1 hour, then probed with a mixture of rabbit anti-FCGRT polyclonal antibody (Atlas Antibodies) at a concentration of 0.15 ug/ml and rabbit anti-B2M onal antibody (Atlas Antibodies) at a concentration of 0.23 ug/ml in TBS+T. The ne was subsequently ted with stabilized goat anti-rabbit antibody conjugated with horse radish peroxidase (Pierce) diluted 1:10,000 in TBS+T. After addition of TMB Substrate (Pierce), an image of the membrane was ed on Amersham Hyperfilm ECL (GE Healthcare). The Hyperfilm was processed using GBX developer and GBX fixer (Sigma-Aldrich).
Production of a non-complexed form of human B2M: Human B2M was produced in E. coli. The expression and purification was performed essentially as described in Sandalova et al. (2005) Acta Chryst F61 :1090- 1093 and lsson et al. (2001) J Immunol 27-7334. The purified protein, consisting of amino acids 21-119 of human B2M, in urea was subjected to arginine refolding as follows; 0.5 mg of B2M was rapidly added to 2 ml refolding buffer (20 ml 1 M Tris-HCI pH 8.0, 16.87 g L-Arginine (buffered with HCI), 0.8 ml 0.5 M EDTA, 61 mg GSSG, 307 mg GSH and milli- Q water to a final volume of 200 ml, pH 8.0, and supplemented with protease tor (Roche, cat. no. 11 873 580 001)). The refolding procedure was performed at 4 °C during 4 hours. Refolded B2M protein was buffer exchanged to PBS using a PD-10 column (GE Healthcare).
W0 2016;042083 2015/071339 Results Construction of plasmids containing the genes for human chRn and human fi2—microglobulin to be used for co-expression: Genes encoding the extracellular domain of the q—chain of human FcRn (chRnECD) and human 82M were inserted into the lentiviral transfer plasmids 2K7bsd and 2K7neo, respectively. In both cases, the inserted gene is under the control of a CMV er. The genes were extended so that the resulting proteins would have an lg kappa chain leader sequence in the N-terminus to target the protein for export through the endoplasmic reticulum to the culture medium (the signal sequence was cleaved upon secretion). In addition, chRnECD had a C- terminal spacer sequence followed by a FLAG-tag for potential detection.
Human 82M had a inal spacer ce followed by a Hise tag for potential detection. The spacer sequence was added to enhance accessibility of the tag. The lentiviral transfer plasmids also contained two different antibiotic resistance genes to allow ion of cells where both constructs had been inserted.
Expression and purification of recombinant human FcRn: The genes encoding chRnECD and BZM were ed into the genome of SKOV—3 by lentiviruses, and the resulting FcRn protein was secreted into the culture medium. To capture only FcRn having retained pH-dependent lgG binding, affinity chromatography using immobilized lgG was used where the or was captured at pH 5.8 and eluted at pH 8.1. ed protein was eluted in three fractions.
SDS-PAGE and Western blot: To investigate the presence of two peptide chains (chRnECD and 82M) of the produced FcRn protein, and to analyze the purity of the eluted material, an SDS—PAGE analysis was performed on the eluted ons. For the gel d with GelCode Blue Stain, two bands were detected with molecular weights of 12 and 36 kDa, respectively. This corresponds approximately to the tical molecular weights of the non-glycosylated peptide chains of 12 kDa for 82M and 31 kDa for chRnECD. The chRnECD part of the protein contains one glycosylation site and it was therefore expected that its molecular mass would be higher than 31 kDa. The gel was also silver stained to increase sensitivity and possibly detect impurities. A band of approximately 66 kDa was detected in the first eluted fraction, which could correspond to BSA (bovine serum n) originating from cell attachment. The total amount of protein recovered in fraction 2 and 3 corresponded to 1.4 mg/l e medium. A W0 2016;042083 western blot analysis on the pooled material was carried out, which showed essentially only the two major bands and in addition a very weak band below 12 kDa which might correspond to a degradation product.
Example 2 Selection and ELISA binding of FcRn binding Z variants In this Example, human FcRn was used as target in phage y selections using a phage library of Z variants. Selected clones were DNA sequenced, produced in E. coli periplasmic fractions and d against FcRn in ELISA (enzyme-linked immunosorbent assay).
Materials and s ylation of target protein FcRn and of B2M: Human FcRn and human B2M, produced as described in Example 1, were ylated using No-Weigh EZ-Link Sulfo-NHS—LC-Biotin (Pierce, cat. no. 21327) at a 31 x (FcRn) and 10 x (B2M) molar , respectively, according to the manufacturer’s recommendations. The reactions were performed at room temperature (RT) for 30 min. Subsequent buffer exchange to PBS was performed using Slide-a-lyzer dialysis cassettes (FcRn; Pierce, cat. no. 66380, 10,000 MWCO and B2M; Pierce, cat. no. 66333, 3,500 MWCO), according to the manufacturer’s instructions.
Phage display selection of FcRn binding Z variants: A library of random variants of n Z displayed on bacteriophage, constructed in phagemid pAY02592 essentially as bed in Grénwall et al. (2007) J Biotechnol, 128:162-183, was used to select FcRn binding Z variants. In this library, an albumin binding domain (ABD, GA3 of protein G from ococcus strain G148) is used as fusion partner to the Z variants. The library is denoted Zlib006Naive.l| and has a size of 1.5 x 1010 library members (Z variants).
E. coli RRIAM15 cells (RUther et al., (1982) c Acids Res 10:5765-5772) from a glycerol stock containing the phagemid library Zlib006Naive.l|, were inoculated in 20 l of a defined proline free medium [dipotassium hydrogenphosphate 7 g/l, trisodium citrate dihydrate 1 g/l, uracil 0.02 g/l, YNB (DifcoTM Yeast Nitrogen Base w/o amino acids, Becton Dickinson) 6.7 g/l, glucose monohydrate 5.5 g/I, L—alanine 0.3 g/l, L-arginine monohydrochloride 0.24 g/l, L-asparagine monohydrate 0.11 g/l, L-cysteine 0.1 g/l, L-glutamic W0 2016;042083 acid 0.3 g/l, L-glutamine 0.1 g/l, glycine 0.2 g/l, L-histidine 0.05 g/l, L- isoleucine 0.1 g/l, L-leucine 0.1 g/l, L-lysine monohydrochloride 0.25 g/l, L- methionine 0.1 g/l, L-phenylalanine 0.2 g/l, L—serine 0.3 g/l, L-threonine 0.2 g/l, L-tryptophane 0.1 g/l, L-tyrosine 0.05 g/l, L-valine 0.1 g/l], supplemented with 100 ug/ml llin. The cultivations were grown at 37 °C in a fermenter (Belach Bioteknik, BR20). When the cells d an optical density at 600 nm (ODsoo) of 0.75, approximately 2.6 l of the cultivation was infected using a x molar excess of M13K07 helper phage (New England Biolabs, cat. no.
N0315S). The cells were incubated for 30 minutes, whereupon the fermenter was filled up to 20 | with TSB-YE (Tryptic Soy Broth-Yeast Extract; 30 g/l TSB, 5 g/l yeast extract) supplemented with 100 uM isopropyl-B-D thiogalactopyranoside (IPTG) for ion of expression and with 25 ug/ml kanamycin and 12.5 ug/ml icillin and grown at 30 °C for 22 h. The cells in the cultivation were pelleted by centrifugation at 15,900 g. The phage particles were precipitated from the supernatant twice in PEG/NaCl (polyethylene glycol/sodium chloride), filtered and ved in PBS and glycerol as described in Gronwall et al., supra. Phage stocks were stored at - 80 °C before use. ions against biotinylated human FcRn were performed in four cycles d in two different tracks. Phage stock preparation and selection procedure were performed essentially as described for selection against another biotinylated target in WO2009/077175. The amplification of phage between the ion cycles was med by infecting E. coli RRIAM15 with phage, then performing cultivation in solution as follows. Eluted phage and 10 x excess of M13K07 helper phage compared to bacteria were d to simultaneously infect log phase bacteria at 37 °C for 30 min without rotation, followed by 30 min with slow rotation. Prior to infection, bacteria were grown to log phase in the defined proline free medium described above.
Infected bacteria were pelleted by centrifugation at 4,300 g for 10 min and resuspended in 200 ml TSB+YE medium mented with 0.1 mM lPTG, ug/ml kanamycin and 100 ug/ml llin and cultivated at 30 °C overnight for phage production.
The selection buffer consisted of 100 mM sodium phosphate and 150 mM sodium chloride adjusted to pH 5.5 with hydrogen chloride and supplemented with 0.1 % gelatin and 0.1 % Tween-20. At selection, human serum albumin (HSA, Albucult, Novozymes) was added to the ion buffer to a final concentration of 1.5 uM. In order to reduce the amount of background binders, pre-selection was performed by incubation of phage stock with Dynabeads® M-280 Streptavidin ads, Dynal, cat. no. 112.06) for 1 hour at RT. A second pre-selection was performed during 30 min at RT against human B2M immobilized in immunotubes (Nunc, cat. no. 444474). 5 pg/ml of human B2M in carbonate buffer (Sigma, cat. no. 068K8214) was lized in the tube at 7 °C for >1 h. After washing twice with tap water, the tubes were blocked with PBS + 0.5 % casein (Sigma, cat. no. C8654) for 30 min at RT before use. All tubes and beads used in the selection were pre-blocked with PBS + 0.1 % gelatin. Selection was performed in solution at RT, followed by capture of target—phage complexes on SA-beads where 1 mg beads per 2.9 pg biotinylated FcRn were used. In cycle 1 of the selections, 100 nM biotinylated FcRn was used and two washes of two min each were performed using selection buffer. An increased stringency, using a lowered target concentration and an increased number of washes, was d in the subsequent cycles: 50 nM/5 washes, 25 nM/8 washes and 10 nM/12 washes were applied in cycle 2, 3 and 4, respectively.
After the washes, bound phage was eluted from the two ion tracks using two different ures; 1) 500 pl 0.1 M glycine-HCI, pH 2.2, followed by immediate neutralization with 50 pl 1 M Tris-HCI, pH 8.0, and 450 pl PBS, or; 2) 500 pl of 100 mM sodium phosphate and 150 mM sodium chloride, pH 8.0 and neutralization with 500 pl PBS.
Seguencing: PCR fragments were amplified from single colonies using a standard PCR program and the primers AFFI-21 (5’-tgcttccggctcgtatgttgtgtg (SEQ ID NO:385)) and AFFI—22 (5’—cggaaccagagccaccaccgg (SEQ ID NO:386)). Sequencing of amplified fragments was performed using the biotinylated oligonucleotide AFFI-72 (5’-biotin-cggaaccagagccaccaccgg (SEQ ID NO:387)) and a BigDye® Terminator v3.1 Cycle Sequencing Kit (Applied Biosystems), used in accordance with the manufacturer’s protocol. The sequencing ons were purified by binding to magnetic streptavidin coated beads (Detach Streptavidin Beads, g, cat. no. 2012-01) using a Magnatrix 8000 (Magnetic Biosolution), and analyzed on ABI PRISM® 3130xl Genetic Analyzer (PE Applied tems).
Production of Z variants for ELISA: Sequenced Z variants were produced by inoculating single colonies from the selections into 10 ml TSB- YE medium supplemented with 100 pg/ml llin and 0.1 mM IPTG and incubating for 24 h at 37 °C. Cells were pelleted by fugation, re- suspended in 2 ml PBST (PBS mented with 0.05 % Tween-20), frozen W0 2016;042083 at —80 °C and thawed in a water bath, to release the periplasmic fraction of the cells. The freeze-thawing procedure was repeated seven times and cells were then pelleted by fugation. The supernatant of the periplasmic extract contained the Z variants as fusions to ABD, expressed as AQHDEALE-[Z#####]—VDYV-[ABD]—YVPG (Gronwall et a/., supra). Z##### refers to dual, 58 amino acid residue Z variants.
ELISA KD analysis of Z variants: The binding of Z variants to FcRn was ed in ELISA assays. Half-area 96-well ELISA plates were coated with 2 ug/ml of an anti-ABD goat antibody (produced in-house) diluted in coating buffer (50 mM sodium carbonate, pH 9.6) at 4 °C overnight. The dy solution was poured off and the wells were blocked with 100 pl of PBSC (PBS supplemented with 0.5 % casein) for 1.5 h at RT. The blocking solution was discarded and 50 pl periplasmic solution, diluted 1:4, was added to the wells and incubated for 1.5 h at RT under slow shaking. The ons were poured off and the wells were washed four times with either 0.05% PCT buffer, pH 6.0 (Mcllvaines phosphate-citrate buffer, pH 6.0, supplemented with 0.05 % Tween-20) or 0.05% PCT buffer, pH 7.4 (Mcllvaines phosphate-citrate buffer, pH 7.4, supplemented with 0.05 % Tween—20). The target protein, biotinylated human FcRn, was added to the wells in a 1:3 diluted concentration series from 2 pg/ml (45 nM) to 0.3 ng/ml (6.9 pM) diluted in PCC buffer, pH 6.0 or pH 7.4, (Mcllvaines phosphate-citrate buffer, pH 6.0 or pH 7.4, supplemented with 0.5 % casein), tively. The plates were incubated for 1.5 h at RT followed by washes as described above. Streptavidin conjugated HRP o Scientific, cat. no. N100) was diluted 1:30 000 in PCC buffer, pH 6.0 or pH 7.4, respectively, and added to the wells followed by 45 min incubation.
After washing as described above, 50 pl lmmunoPure TMB substrate (Thermo Scientific, cat. no. 34021 ) was added to the wells and the plates were treated according to the manufacturer’s recommendations. Absorbance was measured at 450 nm using a multi-well plate reader, Victor3 (Perkin Elmer). A Z variant binding an irrelevant protein was used as negative control and a blank was created by omitting the asmic step. A Z variant which bound to FcRn in a pre-experiment (Z07918, SEQ ID NO:1) was used as positive control. Measured values were ed using GraphPad Prism 5 (GraphPad Software, Inc.) and non-linear regression in order to determine the affinities (KB) of the ctions.
ELISA specificity analysis of Z variants: In another ELISA ment, the specificities of the Z variants were tested by ng them against 2 W0 2016;042083 pg/ml biotinylated human proteins BZM, PSMA (produced in-house) and lgG (polyclonal, Pharmacia) and against PCC buffer pH 6.0 or pH 7.4, respectively. The assay was performed at pH 6.0 and at pH 7.4, respectively, as described above. The biotinylated proteins or buffer were added to the wells instead of FcRn in the target protein step.
Results Phage display selection of FcRn binding Z variants: Individual clones were obtained after four cycles of phage display selections against biotinylated human FcRn.
Seguencing: Sequencing was performed on clones picked at random from ion round four. Each Z variant was given a unique fication number ##### and individual variants are referred to as 2#####. The amino acid sequences of the 58 amino acid residues long Z variants are listed in Figure 1 as SEQ ID NO:1-16 and SEQ ID .
The deduced FcRn binding motifs of these Z variants extend from residue 8 to e 36 in sequences with SEQ ID 6 and SEQ ID NO:353 in Figure 1. The amino acid sequences of the 49 amino acid residues long polypeptides (BMod) predicted to constitute the te three-helix bundle within each of these Z variants extend from residue 7 to residue 55.
ELISA assays with Z variants: Sixteen clones were produced as ABD fusion proteins in E. coli. The periplasmic fractions were used in an ELISA against a dilution series of human FcRn. The clones were: 207909 (SEQ ID NO:13), 207918 (SEQ ID NO:1), 207930 (SEQ ID NO:6), 207960 (SEQ ID N024), 210109 (SEQ ID NO:3), 210111 (SEQ ID N028), 210127 (SEQ ID NO:12), 210129 (SEQ ID NO:9), 210140 (SEQ ID NO:5), 210141 (SEQ ID NO:10), 210145 (SEQ ID NO:15), 210152 (SEQ ID NO:14), 210156 (SEQ ID NO:11), 210161 (SEQ ID NO:16), 210183 (SEQ ID NO:7) and 210193 (SEQ ID NO:2). KD values were ined for all variants at pH 6.0 and for three variants at pH 7.4 (Table 2). For thirteen variants, data was not obtained for a KD is at pH 7.4. None of the sixteen variants displayed non—specific binding when assayed against human B2M, lgG or PSMA.
W0 2016;042083 Table 2. ELISA KD is of Z-ABD variants in E. coli periplasmic fractions. z variant SEQ ID NO: KD pH 5.0 (M) KD pH 7.4 (M) 207909 13 24.5 x10'9 n.d. 207918 1 2.0 x10'9 10.9 x10'9 207930 5 10.4 x10'9 n.d. 207950 4 5.0 x 10'9 n.d. 210109 3 3.9 x 10'9 23.9 x 10'9 210111 8 11.4x10'9 n.d. 210127 12 21.3 x10'9 n.d. 210129 9 17.5 x10'g n.d. 210140 5 8.8 x 10'9 n.d. 210141 10 21.2 x10'9 n.d. 210145 15 42.0 x10'9 n.d. 210152 14 24.5 x10'9 n.d. 210155 11 21.3 x10'9 n.d. 210151 15 153.0 x10'9 n.d. 210183 7 10.9 x10'9 n.d. 210193 2 2.3 x10'9 25.9 x10"g n.d.= not determinable Example 3 Production and characterization of FcRn binding Z variants In this e, seventeen Z variants were produced in E. coli, purified and assayed t human FcRn in Biacore. A subset of said ts was also assayed against mouse FcRn. Circular dichroism (CD) spectroscopy was performed for a subset of Z variants for investigation of their ary structure.
Materials and methods Subcloning of Z variants: The DNA of seventeen FcRn binding Z variants (SEQ ID NO:1-16 and SEQ ID NO:353) was amplified from the library vector pAY02592. A subcloning strategy for construction of ric Z variant molecules with N-terminal Hise tag was applied using standard molecular biology techniques (essentially as described in detail in W02009/077175 for Z variants binding another target). The Z gene fragments were subcloned into the expression vector pAY01448 resulting in the encoded sequence MGSSHHHHHHLQ-[Z#####]—VD.
W0 2016;042083 2015/071339 In addition, the FcRn binding variant 207918 (SEQ ID NO:1), but starting with the amino acids AE instead of VD and denoted 211948 (SEQ ID NO:354), was cloned as homodimeric constructs with two different s between the Z variants and followed by a inal Hise tag. This was performed using conventional molecular biology methods including DNA ication, restriction with suitable restriction enzymes and ligation of the DNA. The two s were obtained from Thermo Fisher Scientific. The Z gene fragments were subcloned into the expression vector (pET-26 origin, Novagen) resulting in the encoded sequence [Z#####]—GT—(G4S)—PR- #]-LEHHHHHH and [Z#####]-GT-(G4S)3-[Z#####]—LEHHHHHH, respectively.
Cultivation and cation: E. coli BL21(DE3) cells (Novagen) were transformed with plasmids containing the gene fragment of each respective FcRn binding Z variant and cultivated at 37 °C in 800 or 1000 ml of TSB—YE medium supplemented with 50 ug/ml kanamycin. At ODBOO = 2, IPTG was added to induce expression at a final concentration of 0.17 or 0.2 mM and the culture was incubated at 37 °C for another 5 h. The cells were ted by centrifugation.
Approximately 2-5 g of each cell pellet was resuspended in 10-25 ml binding buffer (20 mM sodium phosphate, 0.5 M NaCl, 20 mM imidazole, pH 7.4) supplemented with ase® (Merck, cat. no. 1016540001) to a concentration of 15 U/ml and me (Sigma, cat. no. L-7651) to a concentration of 0.5 mg/ml. After cell disruption by three freeze-thawing cycles or sonication, cell debris was removed by centrifugation and each atant was applied on a 1 ml His GraviTrap IMAC column (GE Healthcare, cat. no. 1199). Contaminants were removed by washing with wash buffer (20 mM sodium phosphate, 0.5 M NaCl, 20 or 60 mM imidazole, pH 7.4), and the FcRn binding Z variants were subsequently eluted with elution buffer 1 (20 mM sodium phosphate, 0.5 M sodium chloride, 250 mM imidazole, pH 7.4) or elution buffer 2 (0.1 M acetic acid, 0.5 M sodium chloride, pH 4.5). ed Z variants were buffer exchanged to PBS using PD- columns (GE Healthcare), ing to the manufacturer’s protocol.
Protein concentrations were determined by measuring the absorbance at 280 nm, using a NanoDrop® ND-1000 spectrophotometer, and using the extinction coefficient of the respective protein. The purity of the FcRn binding Z variants was analyzed by SDS-PAGE stained with Coomassie Blue. The W0 2016;042083 identity of each purified FcRn binding Z variant was confirmed using LC/MS analysis.
CD analysis: Purified Hise-tagged Z variants were diluted to 0.5 mg/ml in PBS. For each diluted Z variant, a CD spectrum at 250—195 nm or 250-190 nm was ed at 20 °C. In addition, a variable temperature measurement (VTM) was med to determine the melting temperature (Tm). In the VTM, the absorbance was measured at 221 nm while the temperature was raised from 20 to 90 °C, with a temperature slope of 5 °C/min. A new CD spectrum was obtained at 20 °C after the heating procedure in order to study the refolding ability of the Z variants. The CD measurements were performed on a Jasco J-810 spectropolarimeter (Jasco Scandinavia AB) using a cell with an optical path-length of1 mm.
Biacore binding and c analysis: The ction of FcRn binding Hise-tagged Z variants with human FcRn was analyzed in a Biacore 2000 instrument (GE Healthcare). Human FcRn was immobilized in a flow cell on the carboxylated dextran layer of a CM5 chip surface (GE care). The lization was performed using amine coupling chemistry ing to the manufacturer’s protocol and using HBS—EP (GE Healthcare) as running buffer. One flow cell surface on the chip was ted and deactivated for use as blank during analyte injections. In the two binding experiments presented below, Mcllvaines phosphate-citrate buffer pH 6.0 mented with 0.005 % Tween—20 (0.005 % PCT) was used as running buffer. In all experiments, a flow rate of 50 ul/min was used.
In one experiment, the dissociation at pH 6.0 was compared to the dissociation at pH 7.4. Hiss-tagged Z variants and a human monoclonal IgG1 were d in g buffer to a final concentration of 250 nM or 2.5 nM, respectively, and injected over the FcRn chip for 1 minute using the co-inject procedure. The second injection of the co-inject procedure, representing the dissociation phase of the interactions, contained either running buffer (pH 6.0) or 0.005% PCT pH 7.4. The Z variants were allowed to dissociate for 1 minute, except for 207918 and 210193, which were allowed to dissociate for 4 minutes, before a e equilibration during 5 minutes in g buffer.
IgG was allowed to dissociate for 4 minutes before equilibration. Buffer injections were performed in a similar way; ection of buffer pH 6.0 followed by pH 6.0 or co-injection of buffer pH 6.0 followed by pH 7.4. The results were analyzed in BiaEvaluation software 4.1 (GE Healthcare). Curves of the blank surface were subtracted from the curves of the ligand surface. In on, curves of buffer injections were subtracted from the Z variant curves and from the IgG curves to adjust for the buffer s.
In r ment, approximate kinetic constants (kon and koff) and affinities (KD) were determined for a subset of Hise-tagged 2 variants. Three concentrations of the Z ts were injected for 1 minute followed by dissociation in running buffer for 1 minute. The surfaces were equilibrated with running buffer during 7.5 minutes before the start of next cycle. Injected concentrations were either 675 nM, 225 nM and 75 nM (210140, 210156 and 210183) or 225 nM, 75 nM and 25 nM (207918 and 210193). Kinetic constants were calculated from the sensorgrams using the Langmuir 1:1 model of BiaEvaluation software 4.1 (GE Healthcare).
In a separate experiment, the affinity of the interactions of 2 variants to hFcRn (SEQ ID NO:379) and chRn (SEQ ID NO:384), respectively, was measured at both pH 6.0 and pH 7.4 on a Biacore 3000 ment (GE care). hFcRn and chRn were produced essentially as described in Example 1 but using mouse 3T3 cells instead of human SKOV—3 cells for production of chRn, and immobilized on separate flow cells on a CM5 chip in e buffer at pH 4.65. The immobilization level was approximately 1000 RU for both receptors. A nce flow cell was created by activation and deactivation. 0.005% PCT pH 6.0 or 7.4 was used as running buffer and for dilution of the analytes. All es were performed at 25 °C. The affinity nts for the His6—tagged 2 variants 207918 (SEQ ID NO:1), 207960 (SEQ ID NO:4) and 210193 (SEQ ID NO:2) were determined by injecting a dilution series from 1024 nM to 0.5 nM (pH 6.0) or from 10240 nM to 5 nM (pH 7.4). The affinities were derived using GraphPad Prism 5 software, using a one site binding saturation model.
AlphaLlSA blocking assay: The potential of 2 variants to inhibit binding of IgG to FcRn was analyzed in an AlphaLlSA assay with an EnSpire multiplate reader 2300 (Perkin Elmer). Human IgG (Roactemra) was immobilized on AlphaLlSA acceptor beads (Perkin Elmer, cat. no. 6772002) according to the manufacturer’s recommendations. Stepwise serial dilutions 1:3 of His-tagged 2 variants to final concentrations of 250 nM to 38 pM were made in a 384-well plate n Elmer, cat. no. G6005350) and incubated for 45 min with 10 nM biotinylated human FcRn (Biorbyt, cat. no. orb84388; biotinylated essentially as described in Example 2) in AlphaLlSA buffer (Perkin Elmer, cat. no. ALOOOF) adjusted to pH 6.0 using HCI. IgG—coated Acceptor beads were added to a final concentration of 10 uM and incubated W0 20167042083 for 45 min. Finally, streptavidin coated Donor beads (Perkin Elmer, cat. no. 2) were added to a final concentration of 40 ug/ml and incubated for min. All incubations were performed at RT in the dark. The plate was analyzed in the EnSpire instrument and the |C50 values were calculated using GraphPad Prism 5.
Results Cultivation and purification: The seventeen FcRn binding Z variants (SEQ ID NO:1-16 and SEQ ID NO:353), constructed with an N-terminal Hise tag, were produced in E. coli. The amount of lMAC-purified protein from approximately 2—5 g ial pellets, determined spectrophotometrically by measuring the ance at 280 nm, ranged from approximately 10 mg to mg for the different FcRn binding Z variants. GE analysis of each final protein preparation showed that these predominantly contained the FcRn binding Z variant. The correct identity and molecular weight of each FcRn binding Z variant was confirmed by HPLC—MS analysis.
CD analysis: The CD spectra determined for six Z ts showed that each had an d—helical structure at 20 °C. This result was also verified in the variable temperature measurements, wherein g temperatures (Tm) were determined (Table 3). A reversible folding was seen for the six Z variants when overlaying spectra measured before and after g to 90 °C.
Table 3. Melting temperatures for a selection of Z ts.
Zvariant SEQ ID NO: Tm °C) 207909 13 56 207918 1 49 207930 6 56 207960 4 58 210109 3 61 210193 2 59 Biacore binding and c analyses: The binding of seventeen Hise- tagged 2 variants to human FcRn and the dissociation at different pH were tested in a Biacore instrument by sequentially injecting each of the 2 variants at pH 6.0 and either buffer pH 6.0 or pH 7.4 over a chip surface containing FcRn. The ligand immobilization level of the surface was 1668 RU human FcRn. The seventeen 2 variants showed binding to FcRn at pH 6.0, and for WO 2016042083 all variants, faster tes were seen at pH 7.4 compared to pH 6.0. The result for IgG was similar, ying a faster off-rate at pH 7.4. The variants 207918 and 210193 showed the slowest dissociation curves. Sensorgrams for a subset of variants and IgG are displayed in Figure 2 A-E.
Table 4. Biacore kinetic constants and affinities for hFcRn binding at pH 6.0. 2 variant SEQ ID NO: kon (M'1s'1) km:f (s'1) KD (M) 207918 1 1.4 x106 0.022 1.6 x10'8 210140 5 1.4 x106 0.12 8.6 x10'8 210156 11 7.6 x105 0.28 3.7 x10'7 210183 7 1.0x106 0.13 1.3x10'7 210193 2 1.5 x106 0.033 2.2 x10"8 The kinetic nts of five Z variants interacting with FcRn at pH 6.0 were determined (see Table 4). The immobilization level of the surface was 2015 RU human FcRn. For each Z variant, kinetic constants were calculated using a curve set of three injected concentrations.
Affinity (KD) constants were also determined for Hise-tagged Z variants 207918 (SEQ ID NO:1), 207960 (SEQ ID N024) and 210193 (SEQ ID N022) interacting with human and mouse FcRn at pH 6.0 and pH 7.4 (Table 5). For all three ts, KD values were lower at pH 6.0 compared to pH 7.4.
Table 5. Biacore ties for hFcRn and chRn at pH 6.0 and pH 7.4.
SEQ ID KD (M) hFcRn KD (M) chRn Z variant_ NO: pH 6.0 pH 7.4 pH 6.0 pH 7.4 207918 1 1.2 x10'8 >5 x10'7 9.0 x10'8 >5 x10'7 207960 4 5.0 x10'8 >1 x10'6 3.5 x107 >5 x10'6 210193 2 1.4x10'8 >5x10'7 9.5x10'8 >5x10'7 W0 2016;042083 Table 6: Calculated I050 values from AlphaLlSA blockino assa . z variant SEQ ID NO I050 (M) 207909 _\ 00 4.6 x 10'8 207918 A 2.1 x10'9 |207930 4.2 x 10'8 207960 A 4.2 x 10'8 210109 5.7 x 10'8 210111 4.6x10'8 210140 5.6 x 10'8 210183 3.9 x10'8 210193 1.2x10'8 213993 1.3 x 10'7 211948-(G4S)-211948 3.8 x 10'10 21 1948-(G4S)3-Zi 1948 4.1 x 10'10 AlphaLlSA blocking assay: The y of seventeen Hise-tagged monomeric Z variants (SEQ ID NO:1-16 and SEQ ID NO:353) and two dimeric variant, 211948-G4S-211948 and Z11948-(G4S)3-211948 to inhibit lgG binding to FcRn was tested in an AlphaLlSA blocking assay. Serial dilutions of the Z variants were incubated with biotinylated human FcRn and the blocking ability of each respective variant was measured after addition of IgG coated Acceptor beads and subsequently streptavidin coated Donor beads. Inhibition could be measured as a decrease in AlphaLlSA counts for positive Z variants. The calculated IC50 values for the ten monomeric variants and the two dimeric variants that were shown to block IgG binding to FcRn in this assay are shown in Table 6.
Binding of FcRn binding Z variants to human or mouse FcRn/eGFP transfected HeLa cells In this Example, the binding ability of FcRn g Z variants was investigated. The production of HeLa cells expressing human and murine GFP gene ene and the use of these cells for flow cytometry analysis with Alexa Fluor® 647 d Z variants is described.
Materials and methods Cloning of FcRn-eGFP and 82M viral vectors: The genes encoding murine FcRn (chRn, Genbank BC003786.1, OpenBiosystems) and murine B2M (mB2M, Genbank BC085164.1, OpenBiosystems) were amplified in a similar way as the genes for human FcRn and human B2M as bed in Example 1. Human and murine FcRn and B2M genes were amplified as follows: for hFcRn, the sequence encoding amino acids 1-365 (SEQ ID NO:382) was amplified; for hBZM, the sequence encoding amino acids 21- 119 (SEQ ID NO:380) was ied; for chRn, the sequence encoding amino acids 1—369 (SEQ ID NO:383) was amplified; and for mB2M, the sequence encoding amino acids 21-119 (SEQ ID NO:381) was ied. The vector pHR-cPPT-CMV-EGFP (Jakobsson etal. (2003) J Neurosci Res 73:876-85) and FcRn PCR amplicons (human and murine) were cut using the restriction s BamHl (human) or Bcll (murine) and Mlul (New England Biolabs, cat. nos. R0136M, R0160L and , respectively), and ligated using T4 DNA Ligase (New England Biolabs, cat. no. M0202M). The ligation mix was chemically ormed into E. coli RRIAM15 and spread on ampicillin plates. Colonies were picked and screened with suitable primer pairs. The construct encoding the al signal peptide, human or murine FcRn and eGFP at the cytoplasmic tail were verified by cing and denoted pHR-cPPT-CMV-hFcRn-eGFP and pHR-cPPT-CMV-chRn-eGFP, respectively.
The human and murine B2M PCR amplicons were inserted into the d pDONOR221 (Invitrogen, cat. no. 12536—017) by recombination using the y system (Invitrogen, cat. no. 11789020, Gateway® BP Clonase® II Enzyme mix) according to the manufacturer’s recommendations. After verification of correct sequences, human or murine B2M was inserted into tc (Suter et al., supra) using a multi-site gateway cloning system (Invitrogen, cat. no. 11791020, Gateway® LR Clonase® II Enzyme mix) er with the promoter containing d pENTR-CMV (Tai et al. supra), resulting in the vectors 2k7neo—CMV-hB2M and 2k7neo-CMV-mB2M, respectively.
Lentiviral transduction of HeLa cells: The vector pairs 2k7neo-CMV- hB2M and pHR-cPPT-CMV-hFcRn-eGFP or 2k7neo-CMV-mB2M and pHR- cPPT-CMV-chRn-eGFP were co-transfected together with VSV-G envelope and gag/pol packaging plasmid into HEK293T cells using calcium chloride transfection (Zufferey et al., supra; Jakobsson et al. (2006) supra). HEK293T culture atants containing formed lentiviral particles with FcRn and 82M transgenes respectively were used to sequentially transduce HeLa Cervix adenocarcinoma cells (Cell Line e) at low passage number. The ing two stably transduced HeLa cell lines are in the following d hFcRn-eGFP (transduced with genes for human FcRn-eGFP and hB2M) and chRn—eGFP (transduced with genes for mouse FcRn-eGFP and mB2M).
Alexa Fluor® 647 ng of FcRn binding Z variants: The three Hise- tagged Z variants 207918, 207930 and 207960 were labeled with Alexa Fluor® 647 Carboxylic Acid Succinimidyl Ester (lnvitrogen cat. no. A20106).
Before labeling, buffer was exchanged to 0.2 M carbonate , pH 8.3, using Vivaspin500 centrifugal filter units (10 kDa MWCO, Vivaproducts cat. no. 512—2838) spun at 10,000 g. The labeling was performed in the in500 and 1 pl of Alexa Fluor® 647 Succinimidyl Ester dye (40 pg/pl in DMSO corresponding to 1.3 x molar excess) was added to 200 pg/25 pl Z variant. The mixes were incubated at RT in the dark for 40 minutes in a wiggling rota mixer. The reaction mixes were subsequently put on ice for 3.5 hours and free dye was removed by washing with 15 x 100 pl PBS in the Vivaspin500. lmmunofluorescence staining of human and mouse FcRn-eGFP transfected HeLa-cells with FcRn binding Z variants: hFcRn-eGFP and chRn-eGFP HeLa cells were harvested by trypsination and washed twice in PBS at pH 6.0 before counting. 100,000 cells were ed per well of a v- ed 96 well plate (Nunc, cat no 277143) and the cells in the plate were subsequently pelleted at 1,700 rpm for 4 min at 4 °C. The supernatants were removed and the cells were fixed with 50 pl of 2 % formaldehyde (Sigma Aldrich, cat. no. F8775) in PBS at pH 6.0 for 10 min at RT. Cells were thereafter washed with 2 x 100 pl PBS, pH 6.0, saturated with casein (PBSC), and resuspended in PBSC with 0.1 % saponin (AppliChem, cat no A4518.0100) containing 620 nM of Alexa Fluor® 647 labeled Hiss-tagged Z variants; 207960, 207930 and 207918. Transduced HeLa cells, incubated with buffer alone, were used as l. The cells were incubated for 1 h at 8 °C on a shaker in the dark, washed with 2 x 100 pl PBSC and resuspended in 180 pl of PBS, pH 6.0, containing 1 % BSA (fraction V, Merck, cat. no. 1.12018.0100). 10,000 cells/well were analyzed in a Gallios Flow Cytometer (Beckman Coulter) and the data was analyzed using Kaluza software an Coulter).
W0 2016;042083 Results Flow cytometry analysis was utilized to determine whether the FcRn binding Z variants could bind to human and/or mouse FcRn on human or mouse FcRn/eGFP transduced HeLa cells. The experiment was performed at pH 6.0 with Alexa Fluor® 647 labeled 207960, 207930 and 207918 (SEQ ID N04, 6 and 1, respectively). Dot plot is (y-axis: Alexa Fluor® 647, x- axis: eGFP) showed that the transduced cell tion could be divided into FcRn-eGFP negative and positive population (Figure 3, gate H and l, respectively) indicating heterogeneous expression of the FcRn-eGFP fusion protein by HeLa cells (Figure 3). Accordingly, the mean fluorescence intensity (MFI) values for Alexa Fluor® 647 in gate l were subtracted by background MFI values of Alexa Fluor® 647 in gate H. The calculated MFI values are ted in Figure 4. The results show that 207960, 207930 and 207918 are capable of binding HeLa cells displaying human (Figure 4A) or murine (Figure 4B) FcRn-eGFP.
Blocking of lgG binding to FcRn with the FcRn binding 2 variant 207918 In this Example, the potential competition of FcRn g 2 variants with lgG for binding to FcRn was igated in a cell based assay. Such binding will result in blocking of the lgG-FcRn interaction.
Materials and methods Blocking of lgG-FcRn immunofluorescence ng: Human or murine FcRn-eGFP transduced HeLa cells were prepared as described in Example 4. Fixed cells were resuspended in 50 pl of a mix of either 100 nM Alexa Fluor® 647-conjugated human or mouse lgG (Jackson laboratories, cat. no. 009003 and 0-003, respectively) and 1000, 100, 10, 1 or 0 (buffer control) nM Hise-tagged 207918 d in PBS-casein, pH 6.0, ning 0.1 % saponin (AppliChem). The cells were incubated for 1 h at 37 °C on a shaker in the dark, washed with 2 x 100 pl PBS-casein pH 6.0 and re- suspended in 180 pl of PBS, pH 6.0, containing 1 % BSA. Data from 10,000 cells/well (except somewhat fewer cells for mouse 100 nM mlgG-Alexa Fluor® 647) were obtained using a Gallios Flow Cytometer (Beckman W0 2016;042083 r) and the data was analyzed using Kaluza software (Beckman Coulter).
Results The experiment was performed to ine if the FcRn binding Z variant 207918 (SEQ ID NO:1) blocks the IgG-FcRn interaction. Human or murine FcRn-eGFP transduced HeLa cells were incubated with human or mouse Alexa Fluor® 647-conjugated lgG. The binding was blocked with unlabeled 207918 at different concentrations. Due to the heterogeneous expression of FcRn by the transduced HeLa cells (described in Example 4), the MFI values for Alexa Fluor® 647 in gate N of each sample was cted by the corresponding MFI values in gate M (Figure 5). The t lgG Alexa Fluor® 647 binding was calculated by dividing the different MFI values with the MFI for the blank control. The results showed that 207918 effectively blocked hlgG g to hFcRn (Figure 6A) in a dose dependent manner. rmore, 207918 also blocked mlgG binding to chRn (Figure 6B) although less efficiently ed to hIgG-binding. e 6 Pharmacokinetic study of three FcRn binding 2 variants In this Example, the ability of FcRn binding 2 variants to g serum half-life of a non-specific 2 variant was investigated by a pharmacokinetic study performed in mice.
Materials and methods Subcloning of 2 variants: A subset of 2 variants (207918, 207960 and 210193) was submitted to a second ning. DNA from the subcloned Hise-tagged variants in Example 3 was used as template. First, PCR amplification using suitable primer pairs was performed to create genes encoding 2 variants starting with the amino acids AE instead of VD. The mutated 2 variants are listed in Figure 1 and were denoted 211948 (SEQ ID NO:354), 211946 (SEQ ID NO:355) and 211947 (SEQ ID NO:356), corresponding to mutated 207918, 207960 and 210193, respectively. Genes ng the new 2 variants were restriction cleaved and ligated into a vector harboring the genes encoding albumin binding variant PP013 (SEQ ID W0 2016;042083 ) and 203638 (SEQ ID NO:378) with spacer sequences resulting in a gene fusion encoding [2#####]-GAP(G4S)4TS—[PP013]—GT(G4S)4PR-[ZO3638] (also denoted “Z#####—PP013—203638” or “Z variant in fusion with PP013- 203638”). The negative control molecule [203638]—GAP(G4S)4TS—[PP013] was subcloned in a similar way by ligating 203638 into a vector containing a (G4S)4 linker and the sequence for PP013. The subsequent steps for vector transformation into E. coli were performed as in Example 3. ation and purification: Z variants in fusion with 203638 were produced in E. coli as described in Example 3. Approximately 3 g of each cell pellet was re—suspended in 30 ml TST-buffer (25 mM Tris-HCl, 1 mM EDTA, 200 mM NaCl, 0.05 % Tween20, pH 8.0) supplemented with ase®(Merck). After cell disruption by sonication and clarification by centrifugation, each supernatant was applied on a gravity flow column with 5 ml agarose immobilized with an anti—ABD ligand (see WO2014/064237). After washing with TST-buffer and 5 mM NH4Ac buffer, pH 5.5, the Z variants were eluted with 0.1 M HAc. Acetonitrile (ACN) was added to a final concentration of 10 % to the eluted fractions from the anti-ABD e affinity chromatography cation step and the samples were loaded on a 3 ml ce 15RPC column (GE Healthcare), previously equilibrated with RPC solvent A (0.1 % trifluoroacetic acid (TFA), 10 % ACN, 90 % water). After column wash with RPC solvent A, bound protein was eluted with a linear gradient 0-50 % RPC solvent B (0.1 % TFA, 80 % ACN, 20 % water) during 60 ml. Fractions containing pure Z variant were identified by SDS—PAGE analysis and pooled. After the RPC purification, the buffer of the pools was exchanged to PBS using a HiPrep 26/10 Desalting column (GE Healthcare).
Finally, the Z variants were ed on 1 ml EndoTrap red columns (Hyglos, cat. no. 321063) to ensure low endotoxin content.
Protein concentrations, purities and the identity of each purified Z variant were analyzed as described in Example 3.
Biacore analysis: Expressed and purified Z variants fused to PP013- 203638 were assayed against human FcRn at pH 6.0 essentially as described for the kinetic analysis in Example 3. The 2 variants and the ve control 203638-PP013 were injected at 40 nM, 160 nM and 640 nM during 1 minute ed by dissociation for 2.5 minutes and equilibration for 1 minute. Kinetic nts and affinities were determined for the 2 variants using the luation software.
W0 2016;042083 Pharmacokinetic study: 211947, 211946 and 211948 fused to PP013- 203638 were administered intravenously (i.v.) to male NMRI mice (Charles River, Germany) at a dose of 92 nmol/kg body weight. Sera from groups of three mice were obtained at 0.08, 6, 18, 78, 120, 168 and 240 hours. The concentration of respective Z variant was determined by ELISA.
ELISA: Half-area 96-well ELISA plates were coated at 4 °C overnight with 50 ul/well of an Z specific goat dy (produced in-house) diluted to 4 ug/ml in coating buffer (50 mM sodium carbonate, pH 9.6). The antibody on was poured off and the wells were blocked with 100 pl of PBSC for 1.5 h at RT. The sera were diluted in PBSC containing 1 % mouse serum (matrix) from 1:100 to 1:51 ,200 in a two-fold dilution series in a dilutions plate.
A standard ion for respective Z variant and four quality controls (very low, low, medium and high control) diluted in matrix were included on each plate. 50 ul of the dilutions were transferred per well and the ELISA plates were incubated for 1.5 h at RT. The plates were washed four times with PBST.
Bound Z variants were detected with 50 ul/well of rabbit anti-PPO13 lg (produced in-house) diluted to 4 ug/ml in PBSC. The plates were subsequently incubated for 1.5 h at RT followed by washes as described above. HRP conjugated donkey abbit HRP obtained from Jackson laboratories (cat. no. 711152), d 120,000 in PBSC, was added and the plates were incubated for 1 hour. After washing as described above, 50 ul of ImmunoPure TMB substrate was added to the wells and the plates were developed according to the manufacturer’s recommendations. After 15 minutes of development, the absorbance was ed at 450 nm using a multi-well plate reader (Victor3). The ance values were analyzed using GraphPad Prism 5 to determine the concentrations -spline curve fit) and area under curve (AUC). The concentrations were then plotted as their natural logarithms t time. The resulting curves followed a two compartment model and the terminal half-life was ated as ln2 divided by the slope based on the last three time points.
Results Cultivation and cation: The three FcRn binding Z variants 211947, 211946 and 211948 (SEQ ID NO:356, 355 and 354), constructed as Z#####- PP013-ZO3638, and the negative l ZO3638-PP013, were produced in E. coli. The amount of purified protein from approximately 3 g bacterial pellets, determined spectrophotometrically by measuring the absorbance at 280 nm, W0 2016;042083 ranged from approximately 10 to 25 mg for the ent FcRn binding Z variants. SDS—PAGE analysis of each final protein ation showed that they predominantly ned respective FcRn binding Z variant. The correct molecular weight of each FcRn binding Z variant was med by LC/MS analysis.
Table 7. Kinetic constants and affinities for FcRn at pH 6.0 of Z variants produced as fusions to PPO13-ZO3638. z t SEQ ID NO: k0,,(M'1s'1) k0,.f (5") K5 (M) 211948 354 7.73 x105 0.047 6.2 x108 211946 355 3_35 X105 0.275 82 )(10'7 211947 356 5,54 X105 0.064 9_8 x1o'8 Biacore analysis: The binding to FcRn was analyzed for the three PP013-ZO3638 fused Z variants. The immobilization level of the surface was 548 RU of human FcRn. The resulting rough kinetic constants and affinities for the target binding at pH 6.0 are displayed in Table 7. Fitted curves are displayed in Figure 7A-C. The negative control ZO3638—PP013 was ve against FcRn. cokinetic study: The pharmacokinetic profiles of the above- mentioned constructs of Z variants fused to ZO3638 were compared to the negative control ZO3638—PP013 in a mouse pharmacokinetic study. In previous work, e.g. as described in PCT application WO2009/016043, it is shown that ABD fusion proteins have a long half-life in serum, caused by ABD binding to serum albumin. In accordance with the previous results, terminal half-life of ABD-fused Z variant molecule (ZO3638—PP013) was approximately 43 hours, which is comparable to half—life of mouse albumin (35 hours). The terminal half-lives of the constructs ning FcRn g Z variant molecule in addition to ABD were two— to three-fold longer (Figure 8). The calculated terminal half-lives were 99 hours (211947), 69 hours (211946) and 58 hours (Z11948), suggesting that FcRn binding of the Z variants contributed to the prolonged half-life.
W0 2016;042083 2015/071339 Example 7 Design and construction of a maturation library of FcRn binding Z variants In this Example, a maturated library was constructed. The library was used for selections of FcRn binding Z variants. Selections from maturated libraries are usually expected to result in binders with increased affinity (Orlova et al., (2006) Cancer Res 66(8):4339-48). In this study, randomized single stranded linkers were generated using split-pool synthesis enabling incorporation of defined codons in desired ons in the sis.
Materials and methods Library design: The library was based on the sixteen sequences of the human FcRn binding Z variants in Table 1 and further described in es 2-6. In the new library, 13 variable positions in the Z molecule ld were biased towards certain amino acid residues, according to a strategy mainly based on the binding motifs of the Z variants defined in SEQ ID NO:1-16. A DNA linker was generated using split—pool synthesis containing the 147 bp lly randomized helix 1 and 2 of the amino acid sequence: 5’- AA ATA AAT CTC GAG GTA GAT GCC AAA TAC GCC AAA GAA NNN NNN NNN GCG NNN NNN GAG ATC NNN NNN TTA CCT AAC TTA ACC NNN NNN CAA NNN NNN GCC TTC ATC NNN AAA TTA NNN GAT GAC CCA AGC CAG AGC TCA TTA TTT A -3’ (SEQ ID NO:388; randomized codons are illustrated as NNN) flanked by restriction sites Xhol and Sacl, was d from DNA 2.0 (Menlo Park, CA, USA). The theoretical butions of amino acid residues in the new library, including eight variable amino acid positions (9, 10, 11, 13, 14, 24, 32 and 35) and five constant amino acid positions (17, 18, 25, 27 and 28) in the Z le scaffold are given in Table 8. The resulting theoretical library size is 5.3 x 108 variants. 2015/071339 Table 8: Desion of librar for maturation.
Amino acid Randomization (amino acid Proportion position in the abbreviations) Z variant 9 A,D,E,F,H,|,K,L,N,Q,R,S,T,V,W,Y 1/16 _AF,H 25% ,|,K,,,LNQR,,,,,STVWY 17 ___ —_-— —_-— Library construction: The library was amplified using AmpliTaq Gold polymerase (Applied Biosystems, cat. no. 4311816) during 12 cycles of PCR and pooled products were purified with ck PCR Purification Kit (QIAGEN, cat. no. 28106) according to the supplier’s recommendations. The purified pool of randomized library fragments was ed with restriction enzymes Xhol and Sacl-HF (New England Biolabs, cat. no. R0146L, and cat. no. R3156M) and concentrated using a PCR Purification Kit. uently, the product was subjected to preparative 2.5 % agarose (Nuisieve GTC agarose, Cambrex, lnvitrogen) gel electrophoresis and purified using QIAGEN gel tion Kit N, cat. no. 28706) according to the supplier’s recommendations.
The phagemid vector pAY02592 (essentially as pAffi1 described in ll et a/., supra) was cted with the same enzymes, purified using phenol/chloroform extraction and ethanol precipitation. The restricted fragments and the restricted vector were ligated in a molar ratio of 5:1 with T4 DNA ligase (Fermentas, cat. no. EL0011) for 2 hours at RT, followed by overnight incubation at 4 °C. The ligated DNA was recovered by phenol/chloroform extraction and ethanol precipitation, followed by dissolution W0 2016;042083 in 10 mM Tris-HCI, pH 8.5. Thus, the resulting library in vector pAY02592 encoded Z variants, each fused to an albumin binding domain (ABD) derived from streptococcal protein G.
The ligation ons (approximately 160 ng DNA/transformation) were oporated into electrocompetent E. coli ER2738 cells (50 pl, Lucigen, Middleton, WI, USA). Immediately after oporation, approximately 1 ml of recovery medium (supplied with the ER2738 cells) was added. The transformed cells were incubated at 37 °C for 60 min. Samples were taken for titration and for determination of the number of transformants. The cells were thereafter pooled and cultivated overnight at 37 °C in 1 l of TSB-YE medium, supplemented with 2 % glucose, 10 pg/ml tetracycline and 100 ug/ml ampicillin. The cells were pelleted for 7 min at 4,000 g and resuspended in a PBS/glycerol on (approximately 40 % glycerol). The cells were aliquoted and stored at —80 °C. Clones from the library of Z variants were sequenced in order to verify the content and to evaluate the outcome of the constructed library vis-a-vis the library . Sequencing was performed as described in Example 1 and the amino acid distribution was verified.
Preparation of phage stock: Phage stock containing the phagemid library was prepared in a 20 l fermenter (Belach Bioteknik). Cells from a ol stock containing the phagemid library were inoculated in 10 l of TSB— YE (Tryptic Soy Broth-Yeast Extract; 30 g/l TSB, 5 g/l yeast extract) mented with 1 gil glucose, 100 mg/l ampicillin and 10 mg/l tetracycline.
When the cells reached an optical density at 600 nm (OD600) of 0.6, approximately 1.5 l of the cultivation was infected using a 5 x molar excess of M13K07 helper phage. The cells were ted for 30 min, whereupon the fermenter was filled up to 10 l with x fermentation medium [2.5 g/l (NH4)2SO4; 5.0 g/l yeast extract; 30 g/l tryptone, 2 g/l K2HPO4; 3 g/l KH2PO4, 1.25 g/l; Na3C6H507 - 2 H20; Breox FMT30 antifoaming agent 0.1 mill]. The following components were added: 10 ml carbenicillin 25 mg/ml; 5 ml kanamycin 50 mg/ml; 1 ml 1 M isopropyl-B-Dthiogalactopyranoside (IPTG); 17.5 ml/l of 300 g/l MgSO4, and 5 ml of a trace t solution [35 g/l FeCI3 - 6 H20; 10.56 9/] ZnSO4 - 7 H20; 2.64 g/l CuSO4 - 5 H20; 13.2 g/l MnSO4 - H20; 13.84 g/l CaClz - 2 H20, dissolved in 1.2 M HCI]. A glucose limited fed- batch cultivation was started where a 600 g/l glucose solution was fed to the reactor (3.5 g/h in the start, 37.5 g/h after 20 h and until the end of the ation). pH was controlled at pH 7 through the automatic addition of 25 % NH4OH, air was supplemented (5 l/min), and the stirrer was set at 500 rpm.
W0 2016;042083 After 24 h of fed—batch cultivation the OD600 was 33.2. The cells in the cultivation were pelleted by centrifugation at 15,900 g. The phage particles were precipitated from the supernatant twice in PEG/NaCl, filtered and dissolved in PBS and glycerol as in e 2. Phage stocks were stored at -80 °C until use in selection.
Results Library construction: The new library was designed based on a set of 16 FcRn binding Z variants with verified binding properties (Example 2-6).
The theoretical size of the ed library was 5.3 x 108 Z variants. The actual size of the library, determined by titration after ormation to E. coli ER2738 cells, was 4.5 x 109 transformants.
The library quality was tested by sequencing of 96 transformants and by comparing their actual sequences with the theoretical design. The contents of the actual library compared to the designed library were shown to be satisfying. A maturated library of potential binders to FcRn was thus successfully constructed. e 8 Selection and screening of Z ts from a ted library Materials and s Phage display selection of matured FcRn binding Z variants: The target proteins human FcRn (Biorbyt, cat. no. orb84388) and murine FcRn (Biorbyt, cat. no. orb99076) were biotinylated essentially as described in Example 2 using biotin at 10x molar . Phage display selections, using the new y of Z variant molecules bed in Example 7, were performed in four cycles against human FcRn or murine FcRn essentially as in Example 2 but with the following exceptions. Selection buffers were 0.1% PCTG buffer, pH .5 (Mcllvaines ate-citrate buffer, pH 5.5, supplemented with 0.1 % Tween-20 and 0.1% n) or 0.1% PCTG buffer, pH 7.4, (Mcllvaines phosphate-citrate buffer, pH 7.4, supplemented with 0.1 % Tween-20 and 0.1% gelatin) respectively. Prior to selection, HSA was added to the selection buffers to a final concentration of 1.5 uM. All tubes and beads used in the selection were pre—blocked with either of the two different selections buffers.
A pre-selection step, by incubation of phage stock with SA—beads for 45 min, W0 2016;042083 was performed in cycle 1. For capture of target complexes, 1 mg beads per 1.1 pg biotinylated human FcRn or 1.6 pg biotinylated murine FcRn was used. Washes were performed with 0.1% PCT buffer pH 5.5 or pH 7.4 except for tracks 22-1 and 22-2 where 0.1% PCT supplemented with 25 nM lgG (Herceptin®) or 10 nM lgG, respectively, was used as outlined in Table 9.
The five tracks (1-5) in cycle 1 were divided in the second to fourth cycles, ing in totally seven tracks (1-1 to 5-1) in cycle 2, eleven tracks (1—1-1 to 51) in cycle 3 and en tracks -1 to 1) in cycle 4.
The bound phage particles were eluted as bed in Example 2.
An overview of the selection strategy, describing an increased stringency in subsequent , using a lowered target concentration and an increased number of washes, is shown in Table 9.
Amplification of phage particles: Amplification of phage particles between selection cycle 1 and 2 was performed essentially as bed in Example 2, with the following exceptions. E. coli ER2738 was used for phage amplification and M13K07 helper phage was used in 5 x excess. The amplification of phage particles between the selection cycles 2 and 4 was done by ming infection of bacteria in solution as follows. After infection of log phase E. coli ER2738 with phage particles, TSB supplemented with 2 % glucose, 10 ug/ml tetracycline and 100 ug/ml ampicillin was added, followed by incubation with rotation for 30 min at 37 °C. Thereafter, the bacteria were infected with M13K07 helper phage in 5 x excess. The infected bacteria were pelleted by centrifugation, re—suspended in TSB-YE medium supplemented with 100 uM IPTG, 25 pg/ml kanamycin and 100 ug/ml ampicillin, and grown overnight at 30 °C. The overnight cultures were pelleted in a centrifuge, and phage particles in the supernatant were precipitated twice with PEG/NaCl buffer. Finally, the phage particles were re-suspended in selection buffer before entering the next selection cycle.
In the final selection cycle, log phase bacteria were infected with eluate and diluted before spreading onto TBAB plates (30 g/l tryptose blood agar base, Oxoid cat. no. 0M0233B) supplemented with 0.2 g/l ampicillin in order to form single colonies to be used in ELISA screening.
WO 2016042083 Table 9. Overview of the maturation selection data.
Cycle ion Phage stock Target Target Selection from library or species conc. '0I selection track Zlib006FcRn.l human 100 Zlib006FcRn.l human 1 OO Zlib006FcRn.l human 25 Zlib006FcRn.l murine 100 ZIibOO6FcRn.I murine 100 A human 5O A human 5 [\3 human [\D A human 5 90N human 5 are AA murine 50 murine 10 human 1 O human 5 1}) _\. human 1 human 5 A human 1 O A H"[\J human 5 A human 1 cl»NL human 0.5 A Loo Li's Li's 9"N human 0.25 A -Ib A murine 10 (91 A L '01I |_\ murine 5 AA II _\A—\ III I AA human 1 A I human 0.25 A N—\—\ II I human 0.5 A I N—\—\ III AAAAAA II human 0.1 A I human 1 .0"SJ‘NSJ‘NNWP‘SJ‘S"NWP‘WQNNP‘QP‘NNWNP‘NWV‘N oncn-nonAhmmmm-bmmmm-bhmmm-bhmhmhmmA A iII I iII human O. 01 [>3 A I [)3 A I human 0.25 .“NNWNNNWNWWP‘NNNNQNWNWWWNNQNWNN A4:.ngmehmmmLALLmLmmemLLmngh .0" 01 i G) 'P A I'T’N 2—1-2 human 0.1 .0" O‘l i G) 2—2—1-1 21 and 2—2—2 human 0.5 -4 2—2—2—1 2—2—1 and 2—2—2 human 0.5 “.01.“. Low—h 0"." 01-D- NA 001 W0 42083 Seguencing of potential binders: Individual clones from the different selection tracks were picked for sequencing. All clones run in the ELISA screening were sequenced. ication of gene fragments and sequence analysis of gene fragments were med essentially as described in Example 2.
ELISA screening of Z ts: Single colonies ning Z variants (expressed as Z variant ABD fusion proteins as described in Example 2) were randomly picked from the selected clones of the FcRn maturated library and grown in 1 ml cultivations essentially as described in Example 2. Preparation of the periplasmic supernatants was performed as in Example 2 with eight freeze thawing cycles and the periplasmic fractions were used undiluted in the ELISA ing. ELISA screenings were performed at both pH 6.0 and pH 7.4 essentially as described in Example 2 using biotinylated human FcRn at a concentration of 2 nM in each well. The periplasmic on of the primary FcRn binder 210193 (SEQ ID NO:2; assayed in above experiments) was used as a positive control. Periplasm containing the ABD moiety only was used as a ve control.
ELISA KD analysis of FcRn binding Z variants: A selection of FcRn binders was subjected to an analysis of the response against a dilution series of biotinylated human FcRn using ELISA at both pH 6.0 and pH 7.4 as described above. Biotinylated human FcRn was added at a concentration of nM and diluted stepwise 1:3 down to 14 pM. As a ound control, all Z variants were also assayed with no target protein added. Periplasm samples containing the primary FcRn binder 207918 (SEQ |D.NO:1)was included and analyzed as a positive control. Periplasm containing the ABD moiety only was used as a negative control. Data were analyzed using GraphPad Prism 5 and non-linear regression and KD values (the half maximal effective concentration) were calculated.
Results Phage display ion of maturated FcRn binding Z ts: Selection was performed in totally 14 parallel tracks containing four cycles each. The different selection tracks ed in target concentration, target type (human FcRn or murine FcRn), selection time, and wash conditions.
W0 42083 Sequencing of potential s: Randomly picked clones were sequenced. Each individual Z variant was given an identification number, Z#####, as described in e 2. In total, 445 new unique Z variant molecules were identified.
The amino acid sequences of a subset of the 58 amino acid residues long Z variants are listed in Figure 1 and in the sequence listing as SEQ ID NO:17-352. The deduced FcRn binding motifs of these Z ts extend from residue 8 to residue 36 in sequences with SEQ ID 352. The amino acid sequences of the 49 amino acid es long polypeptides (BMod) predicted to constitute the complete three-helix bundle within each of these Z variants extend from residue 7 to residue 55.
ELISA screening of Z variants: Clones obtained after four selection cycles were produced in 96-well plates and screened for FcRn binding activity using ELISA. All randomly picked clones were analyzed. At pH 6.0, 333 of the 445 unique Z variants were found to give a response of 0.3 AU or higher (corresponding to at least 3x the negative control) against human FcRn at a concentration of 2 nM. At pH 7.4, 278 of the 445 unique Z variants were found to give a response of 0.3 AU or higher (corresponding to at least 3x the negative control) against human FcRn at a concentration of 2 nM. Clones with a positive signal against human FcRn were found in all tracks (including those with murine target) except 11-1. The negative ls had absorbances of 0.070-0.096 AU (pH 6.0) and 112 AU (pH 7.4), respectively. The average response of the blank controls was 0.070 AU (pH 6.0) and 0.062 (pH 7.4).
ELISA KD analysis of FcRn binding Z variants: A subset of Z variants was selected based on the result in the ELISA experiment described above (highest ELISA value at pH 6.0 and/or pH 7.4) and subjected to a target titration in ELISA format. Periplasm samples were incubated with a serial dilution of biotinylated human FcRn. A periplasm sample with the primary binder 207918 (SEQ ID NO:1) was also assayed as a ve control.
Obtained values were analyzed and their tive KD values were calculated (Table 10).
Table 10: Calculated KD values from ELISA titration analysis of Z—ABD variants from the maturation.
SEC! Kn Kn Kn ID pFl&0 pFIZ4 vafiant ch (M) (M) (M) (M) 213574 18 1.2 x 10‘9man—— 213578 20 1-0x10'9 9.8x 10'10 3.6 x 10'9 213581 22 1.1x10'9 3.3 x 10'9 213695 84 1.8x 10'9 5.3 x 10'9 213583 23 8.0x10‘10 1.5x10'9 213697 85 1.2x 10‘9 2.4 x 10'9 213585 24 1.2x10'9 1.7x10'9 213706 2.0x 10'9 6.4 x 10'9 213586 25 1.2x10‘9 2.3x10‘9 213708 87 1.9x 10‘9 4.4 x 10'9 213587 26 '9 6.9x10'9 213710 88 1.6x 10'9 2.6 x 10'9 213588 27 1.0 X 10'9 2.3 X 10'9 213711 2.1 x 10'9 4.9 x 10'9 213592 28 9.5x10‘10 1.8x10'9 213714 2.1x10'9 6.0 x 10'9 213594 29 1.3x10'9 6.3x10'9 213716 91 1.8x10'9 5.8 x 10'9 213596 30 1.5x10‘9 3.6x10'9 213719 92 2.6x 10‘9 7.3 x 10'9 213597 31 1.4x10'9 6.0x10'9 213720 93 2.5x 10'9 4.5x 10'7 213598 32 ‘9 1.7x 10'9 213721 94 1.9x10‘9 2.9 x 10'9 213600 33 1.4x10'9 4.0x10'9 213725 95 1.8x10'9 4.9 x 10'9 213604 43 1.3 x 10‘9 4.1 x 10‘9 213727 ‘9 5.9 x 10-9 213605 35 1.3x10'9 3.8x10'9 213728 97 2.6x10'9 6.7 x 10'9 213609 36 1.3x10'9 2.7x10'9 213732 2.1x10'9 9.4 x 10'9 213611 37 ‘9 2.5x10'9 213735 1.6x10'9 9.1 x 10'9 213612 38 '9 8.6X10'9 213736 100 1.7x10'9 3.0 x 10'9 213613 39 1.2x10‘9 4.3x10'9 213740 101 2.0x10‘9 5.0 x 10'9 213615 40 1.2x10'9 3.1x10'9 213742 102 2.4x 10'9 7.6 x 10'9 213616 41 9.6x10‘10 1.7x10-9 213747 103 1.3x 10‘9 2.3 x 10'9 213617 42 1.2x10'9 1.9x10'9 213749 104 2.8x 10'9 1.2 x 10'8 213620 43 1.4x10'9 3.3x10'9 213750 105 2.7x 10'9 8.4 x 10'9 213621 44 8.6x10‘10 1.4x10'9 213751 106 2.0x10'9 3.8 x 10'9 213622 45 1.1x10'9 2.1x10'9 213752 107 '9 5.8 x 10'9 213624 46 1.3x10‘9 3.4x10'9 213758 108 1.9x10‘9 6.5x 10'9 213625 47 1.3x10'9 2.8x10'9 213759 109 2.1x10'9 5.6 x 10'9 213626 48 1.2x10‘9 2.7x10'9 213760 110 2.1x10‘9 5.8 x 10'9 213627 49 1.2x10'9 2.9x10'9 213761 111 '9 3.7 x 10'9 213628 50 1.3x10‘9 5.5x10‘9 213771 112 1.5x 10‘g 2.0 x 10‘9 213629 51 '9 8.5x10'9 213773 113 2.5x10'9 4.9 x 10'9 213633 52 1.5x10'9 6.2x10'9 213776 114 2.2x 10'9 5.5x 10'9 W0 2016;042083 SEQ KD variant NO: (M) 213637 55 1.3 x109 213639 57 1.3 x 10-9 2.3 x 10'9 4.7 x 10'9 120 2.0 W" 2.9 x10"’ 213641 59 1.1x10‘9 121 2.3x 10-9 4.2x 10'9 213644 60 1.3 x 10-9 122 1.9 x 10'9 5.6 x 10'9 123 ‘9 3.1x10'9 213648 62 1.6 x 10'9 124 2.4 x 10'9 5.5 x 10'9 213651 63 1.2x 10'9 125 2.0x 10'9 3.1 x 10'9 213652 64 1.4x 10'9 126 2.3x10'9 1.1x10'8 127 2.9 x109 3.8 x10"’ 213655 66 1.1x10‘9 128 1.9x 10-9 3.8x 10'9 213656 67 1.1 x 10'9 129 2.6x 10'9 5.4x 10'9 130 2.2 x109 4.1x10'9 213659 69 2.2 x 10'9 131 2.2 x 10'9 5.5 x 10'9 132 2.6 X109 42 x199 213664 71 2.4 x 10'9 133 2.3 x 10'9 4.3 x 10'9 134 2.1x10'9 3.1x10'9 213669 73 9.2 x 10-10 135 2.1 x 10'9 3.0 x 10'9 213672 74 2.5 x 10'9 136 2.3 x 10'9 8.7 x 10'9 137 2.5 X109 56 x10'9 213675 76 9.6 x 10'10 138 2.0 x 10'9 2.8 x 10'9 139 2.0 x10‘9 3.4 x10'9 213678 78 2.0x10'9 140 2.1 x 10'9 3.0x 10'9 W0 2016;042083 Example 9 tion and characterization of Z variants from a maturated library In this Example, twelve 2 variants were produced in E. coli, purified and assayed for stability, for binding to FcRn as well as for inhibition of lgG g to FcRn. als and methods Subcloning of Z ts into expression vectors: The DNA of twelve FcRn binding Z variants (213577 (SEQ ID NO:19), 213578 (SEQ ID NO:20), 213583 (SEQ ID NO:23), 213592 (SEQ ID NO:28), 213616 (SEQ ID NO:41), 213621 (SEQ ID NO:44), 213654 (SEQ ID NO:65), 213663 (SEQ ID NO:70), 213669 (SEQ ID NO:73), 213674 (SEQ ID , 213675 (SEQ ID NO:76) and 213676 (SEQ ID ) was amplified from the library vector pAY02592. The subcloning was performed as described in Example 3. The 2 gene fragments were subcloned into the expression vector pAY01448 resulting in the encoded ce MGSSHHHHHHLQ-[2#####]-VD.
Production of 2 variants: Cultivation and purification of the Hiss-tagged 2 variants were performed essentially as described in Example 3. In order to obtain higher purity, a ed phase chromatography (RPC) step was added after the IMAC purification of a second batch of the twelve matured variants and the primary 2 variant 207918. Samples from this batch were used where indicated.
CD analysis: In order to determine the melting temperatures (Tm) and assess the secondary structure of the 2 variants (RPC purified batch), CD analysis was carried out as described in Example 3.
Biacore binding and c analyses: The interaction of FcRn binding Hise-tagged 2 variants with human FcRn was analyzed in a Biacore 2000 instrument essentially as described in Example 3. Human FcRn (hFcRn) or lgus FcRn (chRn) purchased from t (cat. no. orb84388 and orb99075, respectively) were used as target protein. In a first set of experiments, 100 nM of the 2 variants was ed at pH 6.0 during 2 min at ul/min over immobilized hFcRn followed by dissociation in buffers of pH 6.0 or pH 7.4 using the co-inject procedure. The dissociation phase was 4 min and the equilibration time between the analyte injections was 30 min.
In a second set of experiments, approximate kinetic constants (kon and koff) and affinities (KD) were determined for a subset of 2 ts injected at W0 2016;042083 concentrations of 540 nM, 180 nM, 60 nM, 20 nM and 6.7 nM over immobilized hFcRn. As above, the analytes were injected during 2 min at 30 pl/min, the dissociation phase was 4 min and the equilibration time between the analyte injections was 30 min.
In a third set of experiments, a kinetic analysis of the twelve matured Z ts and the primary Z variant 207918 (SEQ ID NO:1) (RPC ed batches) binding to hFcRn and chRn was performed at pH 6. A concentration series of Hiss-tagged Z variants (270, 90, 30 and 10 nM) were injected during 4 min at 30 pl/min over hFcRn and chRn, immobilized in different flow cells of a CM5 chip surface. 0.005 % PCT pH 6.0 was used as g buffer and for dilutions of the agged Z variants. Dissociation in running buffer was allowed for 20 min, followed by e regeneration by injection of 3 x 30 second pulses of 0.005 % PCT pH 7.4 and equilibration ten minutes before the start of next cycle.
AlphaLlSA blocking assay: The potential of Z variants to inhibit g of lgG to FcRn was analyzed in the AlphaLlSA assay described in Example 3.
Results Production of Z variants: The twelve FcRn binding Z variants constructed with an N—terminal His6 tag were produced in E. coli. SDS—PAGE analysis of each final n preparation showed that these predominantly contained the FcRn binding Z variant. The correct identity and lar weight of each FcRn binding Z variant was confirmed by HPLC-MS analysis.
CD analysis: Determined melting temperatures are shown in Table 11. ible folding was seen for all FcRn binding Z variants when overlaying spectra measured before and after heating to 90 °C.
Biacore binding and kinetic analyses: In a first set of experiments, the g of the twelve Z variants to human FcRn and the dissociation at different pH were tested in a Biacore instrument by sequentially injecting each of the Z variants at pH 6.0 and either buffer pH 6.0 or buffer pH 7.4 over a chip surface containing FcRn. The ligand immobilization level of the surface was 890 RU human FcRn. The twelve Z variants showed binding to FcRn at pH 6.0, and for all variants, faster off-rates were seen at pH 7.4 compared to pH 6.0.
W0 20167042083 The kinetic constants of the Z variants 213577 (SEQ ID NO:19) and 213621 (SEQ ID NO:44) interacting with FcRn at pH 6.0 were determined in a second set of experiments (see Table 12). c constants were calculated using curve sets of two or four injected concentrations of 213577 and 213621, respectively.
Table 11: Meltino temperatures for a set of matured FcRn bindino 2 variants.
Z variant SEQ ID NO: 213577 _\ O 03 _\ 213578 [\J O 01V 213583 N03 m A 213592 NO) 01 00 213616 4:. _\ O)O 213621 44 49 213654 03 U1 01 00 213663 \lO 030 213669 ‘1 00 4s 01 213674 \l 01 010 213675 \I O? A00 213676 ‘1 ‘1 4s 01 207918 _\ 4s (0 Table 12. Biacore c constants and ties for FcRn binding at pH 6.0.
Zvariant SEQ ID NO: kon(M"s") km:f (s'1) KD (M) 213577 19 3.0 x105 4.0x10'3 13x10'9 213621 44 6.4 x105 3.7x10'3 6X10'9 In a third set of experiments, the kinetic constants of thirteen Hise— tagged 2 ts interacting with human or cynomolgus FcRn at pH 6.0 were determined (Table 13). The FcRn immobilization levels of the chip surfaces were 1196 RU (human) and 788 RU (cynomolgus), respectively. For each 2 variant, c constants were calculated using a curve set of four injected concentrations.
W0 2016;042083 Table 13. Biacore kinetic constants and affinities for human and cynomolgus FcRn binding at pH 6.0. hFcRn chRn z variant ID kon (M'1s'1) koff (5") KD (M) kon (M'1s'1) koff (5'1) KD (M) 213577 19 7.2 x105 2.9 x10'3 4.1 x10'9 8.2 x105 4.4 x10'3 5.4 x10'9 213578 20 4.1x105 ‘3 1.8x10'8 5.6x105 1.1x10'2 2.1 x10'8 213583 23 4.5 x105 2.6 x10'3 5.8 x10'9 6.7 x105 4.4 x10'3 6.6 x10'9 213592 28 5.9x105 7.2x 10‘3 1.2x 10'8 7.5x105 '2 1.5x10'8 213616 41 2.9x105 3.1 x10'3 1.0x10'8 4.3x105 4.8x10'3 1.1x10'8 213521 44 4.1x105 2.8 x10‘3 6.8 x10'9 6.1 x105 4.6 x10'3 7.6 x10'9 213654 65 6.0x105 9.5x10'3 1.6x10'8 8.4x105 1.3x10'2 '8 213563 70 3.9 x105 3.4 x10‘3 8.7 x10'9 5.2 x105 5.3 x10'3 1.0 x10'8 213669 73 5.6 x105 2.8 x10'3 4.9 x10'9 8.2 x105 4.6 x10'3 5.6 x10'9 213574 75 5.3 x105 3.7 x10‘3 7.0 x10'9 8.3 x105 5.9 x10'3 7.1 x10'9 213675 76 4.9x105 5.1 x10'3 1.0x10'8 7.5x105 8.0x10‘3 1.1x10'8 213576 77 6.5 x105 3.6 x10‘3 5.5 x10'9 9.6 x105 5.9 x10'3 6.2 x10'9 207918 1 2.6x105 4.2x10'3 1.6x10'8 3.8x105 7.0x10‘3 1.9x10'8 Table 14: Calculated IC50 values from AlphaLlSA no assa .
Z variant SEQ ID NO: 213577 213578 213583 213592 [\JNNA OOOOOCQ 213616 213621 213654 213663 \IO-D- 001-5 213669 ‘1 00 213674 \l 01 213675 \l 03 213676 \l ‘1 AlghaLlSA blocking analysis: The ability of twelve maturated Hisstagged monomeric Z ts to inhibit lgG binding to FcRn was tested in an WO 42083 AlphaLlSA blocking assay. Serial dilutions of the Z variants were incubated with biotinylated human FcRn and the blocking ability of each respective variant was measured after on of lgG coated Acceptor beads and subsequently avidin coated Donor beads. Inhibition could be measured as a decrease in AlphaLlSA counts for positive Z variants. All twelve tested Z variants were shown to block lgG binding to FcRn and the calculated IC50 values are shown in Table 14.
Example 10 Comparison of blocking capacity of lgG binding to FcRn In this Example, the lgG blocking capacity of the FcRn g Z variant O7918 (SEQ ID NO:1) was compared to Intravenous immunoglobulin (Mg) and Subcutaneous globulin (SClg) currently used in the treatment of some autoimmune disorders.
Materials and methods Blocking of lgG-FcRn immunofluorescence staining: Human or murine FcRn-eGFP transduced HeLa cells were prepared as described in Example 4. Fixed cells were resuspended in 50 pl of a mix of 50 nM Alexa Fluor® 647- ated human lgG (Jackson laboratories, cat. no. 009-600—003) and Hise— tagged 207918, lVlg (Octagam®, Octapharma) or SClg (Gammanorm®, arma), respectively, diluted at concentrations of 1000, 100, 10, 1, 0.1 or 0 (buffer control) nM in Mcllvanes buffer pH 6.0, containing 2.5 % FBS Ultra low lgG (Life Technologies) and 0.1 % n (AppliChem). The cells were incubated for 1 h at 37 °C in the dark, washed with 2 x 100 pl Mcllvanes, pH 6.0, containing 2.5 % FBS Ultra low lgG and re-suspended in 180 pl of Mcllvanes, pH 6.0, containing 1 % BSA. Data from 10,000 GFP/FcRn positive cells were obtained using a FACS Calibur (Beckman Coulter) and the data was analyzed using Flowing software 2.5.0 (Turku University).
Results The experiment was performed to determine if the FcRn binding Z variant Hiss-ZO7918 (SEQ ID NO:1) blocks the lgG-FcRn interaction and compare the blocking effect to Mg and SClg. Human or murine FcRn-eGFP transduced HeLa cells were incubated with human Alexa Fluor® 647- W0 42083 conjugated lgG. The binding was d with unlabeled Hise-Z07918, lVlg or SClg at different trations. The results showed that Hise-ZO7918 effectively blocked hlgG binding to hFcRn to a similar extent as |V|g or SClg (Figure 9).
Example 11 Increased lgG catabolism by FcRn binding Z variants in mice The ability of the FcRn binding Z variant ZO7918 to block lgG binding to FcRn in vitro was shown in Example 10. In this example, the blocking ability of the same Z variant was evaluated in vivo. Blocking of lgG-FcRn interactions in vivo will lead to increased lgG catabolism and concomitant d levels of lgG (Mezo 2008, supra).
Materials and methods Animal study: The FcRn-binding Z variants Z11948 (SEQ ID NO:354) and ZO7918-PP013 8 (SEQ ID NO:1) identical to Z11948 but with the N-terminus ng with the amino acids VD instead of AE, in fusion with the ABD variant PPO13 (SEQ ID NO:377)) or vehicle (PBS buffer), were administered to male NMRI (Charles , at a dose of 16.3 umol/kg. The mice were treated with five intravenous injections given at 0, 24, 48, 72 and 96 h. Serum samples were taken at 0, 72, 120 and 168 h (termination of study) and stored at -20 °C. The concentration of mouse lgG in serum was quantified by ELISA.
Mouse lgG ELISA: The concentration of mouse lgG in mouse serum s was analyzed by a mouse lgG ELISA kit (Mabtech 3825-1AD-6) and performed as described by the manufacturer. The concentration of mlgG was calculated from a standard curve provided and GraphPad Prism 5 using a non-linear regression formula. The concentration of lgG in individual mice at 24, 72, 120 and 168 h were related to the level at 0 h and the results are therefore presented as percentage of lgG (O h).
Results The results showed a reduction of mouse lgG concentration in mice treated with pecific Z variants. Both Z11948 and the ABD-fused variant Z07918-PP013 lowered the concentration of endogenous lgG in mice in vivo.
W0 2016;042083 Most pronounced effects were obtained with the ABD-fused t and after 120 hours. Thus, the results indicates that the FcRn-specific Z variants blocked recycling of lgG resulting in increased lgG catabolism and uent lower levels of lgG in mice. e 12 In vitro transcytosis of FcRn binding 2 ts In this Example, the FcRn binding Z ts are tested for their y to be orted through epithelial or endothelial cells or recycled by FcRn in vitro. A drug containing a Z variant with the power of transcytosis will facilitate drug uptake after for example oral or pulmonary administration.
Materials and methods Cells, for example T84, MDCK, HeLa, CaCoZ, CaLu-1 and/or CaLu-3 cells, with or without endogenous or recombinant expression of FcRn, are grown in respective growth medium on a membrane in a transwell to form a monolayer. The integrity of monolayers can be evaluated by measuring the electrical resistance or adding a probe that is not able to penetrate or being actively transported over the cell monolayer. A defined monolayer of cells is pulsed from the apical or basolateral side with ligand such as FcRn binding Z variants, HSA or lgG in a buffer such as HBSS ’ Balanced Salt Solution, SigmaAldrich, cat. no. H9269) or growth medium at a suitable pH and temperature, and chased with buffers such as HBSS or growth medium at a suitable pH and temperature on the opposite side.
In a variant of this assay, ligands can be chased with buffers such as HBSS or growth medium at suitable pH and temperature on the same side as administration to measure recycled ligand as well. This can be done in a transwell or in a cell culture dish. Cells are seeded into transwell or cell culture dishes and pulsed with ligands such as FcRn binding Z ts, HSA or lgG. Endocytosed ligands will bind to FcRn and return to the cell surface at the same or opposite side as they were loaded. After pulsing, free ligands are removed by g the cells with cold buffer. To chase ligands, warm buffer or medium is added to the cells and, after a period in the range from 10 minutes to several hours, the buffer or medium is removed and assayed for the presence of ligands.
W0 2016;042083 In a variant of this assay, ligands such as FcRn binding Z variants, HSA or lgG can be used to block the binding to FcRn by ligands such as other FcRn binding Z variants, HSA or IgG by administering them at the same time or sequentially to the cells.
The amount of ligand can be quantified by methods such as ELISA, HPLC-MS, fluorescent dye or radio labeling.
The results of the experiment described above are ed to show that the FcRn-specific 2 variants can be transcytosed and/or recycled in vitro.
Example 13 Binding of homodimeric FcRn binding polypeptides to human FcRn/eGFP transfected HeLa cells In this Example, the g y of homodimeric FcRn g polypeptides was investigated and compared to the binding ability of monomeric primary and maturated Z variants. The production of HeLa cells expressing human FcRn-eGFP gene transgene was performed as described in e 4 and the use of these cells for flow try analysis with Alexa Fluor® 647 d Z variants is described.
Materials and methods Alexa Fluor® 647 labeling of FcRn binding polypeptides: The two homodimeric Hise-tagged ptides 211948-(G4S)3—211948 (SEQ ID NO:369) and Z11948—(G4S)-211948 (SEQ ID NO:368), the primary monomeric Hise-tagged Z t 207918 (SEQ ID NO:1) and the maturated monomeric Hise-tagged Z-variants 213583 (SEQ ID NO:23), 213621 (SEQ ID NO:44), 213654 (SEQ ID NO:65) and 213674 (SEQ ID NO:75) were labeled with Alexa Fluor® 647 Carboxylic Acid Succinimidyl Ester (Invitrogen cat. no.
A20106). Before labeling, the pH in the sample suspensions (in PBS pH 7.4) was adjusted to 8.3 by addition of 10 ul of 0.1 M sodium bicarbonate buffer, pH 8.3, to 90 ul sample suspension. 10 pl of Alexa Fluor® 647 Succinimidyl Ester dye (10 mg/ml in DMSO corresponding to 4 x molar excess) was added to 100 pl of each sample suspension. The mixes were incubated at RT in the dark for 1 h in a wiggling rota mixer. The reaction mixes were ately transferred to dialysis cassettes (3500 MWCO) (Thermo Scientific cat. no. 66333) and free dye was removed by dialysis in PBS pH 7.4.
W0 2016;042083 Immunofluorescence staining of human FcRn-eGFP transfected HeLa- cells with FcRn g polypeptides: hFcRn-eGFP HeLa cells were harvested by trypsination and washed twice in Mcllvanes buffer, pH 6.0 before counting. 100,000 cells were pipetted per well of a v-bottomed 96 well plate (Nunc, cat no 277143) and the cells in the plate were subsequently pelleted at 1,700 rpm for 4 min at 4 °C. The atants were d and the cells were fixed with 50 ul of 2 % formaldehyde (Sigma h, cat. no.
F8775) in Mcllvanes buffer for 10 min at RT. Cells were thereafter washed with 2 X 100 pl Mcllvanes buffer, pH 6.0, containing 2.5 % FBS Ultra low lgG (Life Technologies), and resuspended in nes buffer, pH 6.0, containing 2.5 % FBS Ultra low lgG and 0.1 % saponin (AppliChem, cat no A4518.0100) ning 640 nM of Alexa Fluor® 647 labeled Hise-tagged polypeptides; Z11948-(G4S)3-211948 and 211948-(G4S)—211948 and 207918. uced HeLa cells, incubated with buffer alone, were used as control. The cells were incubated for 1 h at 8 °C on a shaker in the dark. The cells were then ted to two different washing conditions; 2 x 150 pl Mcllvanes buffer, pH 6.0, containing 2.5 % FBS ultra low lgG or 2 X 150 pl PBS, pH 7.4, containing 2.5 % FBS Ultra low lgG and a 20 min incubation step in PBS, pH 7.4, ning 2.5 % FBS Ultra low lgG. After washing, all samples were re- ded in 180 pl of Mcllvanes, pH 6.0, containing 2.5 % FBS Ultra low lgG. Data from 10,000 GFP/FcRn positive cells were obtained using a FACS Calibur (Beckman Coulter) and the data was analyzed using Flowing software 2.5.0 (Turku sity).
Results Flow cytometry analysis was utilized to determine whether FcRn binding dimers could bind to human FcRn on human FcRn/eGFP transduced HeLa cells and to compare their binding ability to the monomeric FcRn binding Z variants. The analysis was also performed to determine if the pH dependent detachment from the FcRn protein was affected by the dimeric format. The experiment was performed at pH 6.0 with washings at pH 6.0 or pH 7.4 with Alexa Fluor® 647 labeled dimers 211948-(G4S)3-211948 and 211948-(G4S)—211948, and monomers 207918, 213583, 213621, 213654 and 213674. 211948 and 207918 are identical in sequence apart from the first two amino acid residues (AE vs VD). The calculated MFI values are presented in Figure 11. The results show that the dimeric format increases the binding capacity of the FcRn g polypeptides compared to the corresponding monomer (Figure 11A) and that the maturated Z variants (213583, 213621, 213654 and 213674) have a higher binding capacity than the primary 2 variant 207918 (Figure 11B). The data shows that the pH dependent detachment from FcRn decreases with the use of the dimeric format, suggesting that FcRn binding dimers may have an improved pH dependent binding profile compared to corresponding monomeric variants.
Example 14 Comparison of blocking capacity of IgG binding to FcRn In this Example, the IgG blocking ty of the FcRn binding dimers Z11948-(G4S)3-211948 and Z11948-(G4S)—211948 was compared to that of monomeric FcRn binding 2 variants, as well as to intravenous immunoglobulin (Mg) and subcutaneous immunoglobulin (SCIg) currently used in the ent of some mune disorders.
Materials and methods Blocking of IgG-FcRn immunofluorescence staining: Human FcRn- eGFP transduced HeLa cells were prepared as bed in Example 4.
Fixed cells were ended in 50 ul of a mix of 50 nM Alexa Fluor® 647- conjugated human lgG (Jackson tories, cat. no. 009—600-003) and His;- tagged 211948-(G4S)3-211948 (SEQ ID NO:369), 211948-(G4S)—211948 (SEQ ID NO:368), 207918 (SEQ ID NO:1), 213583 (SEQ ID NO:23), 213621 (SEQ ID NO:44); IVIg (Octagam®, Octapharma) or SCIg (Gammanorm®, Octapharma), respectively, diluted at trations of 1000, 100, 10, 1, 0.1 or 0 (buffer control) nM in Mcllvanes buffer, pH 6.0, containing 2.5 % FBS Ultra low IgG (Life Technologies) and 0.1 % saponin (AppliChem). The cells were incubated for 1 h at 37 °C in the dark, washed with 2 x 100 pl Mcllvanes , pH 6.0, containing 2.5 % FBS Ultra low lgG and re-suspended in 180 pl of nes buffer, pH 6.0, containing 1 % BSA. Data from 10,000 GFP/FcRn positive cells was obtained using a FACS Calibur (Beckman Coulter) and analyzed using Flowing software 2.5.0 (Turku University).
Results The experiment was performed to determine if the FcRn binding dimers 211948-(G4S)3-211948 and 211948-(G4S)-211948 block the IgG— FcRn interaction, and e the blocking effect to that of the monomeric FcRn binding Z variants 207918, 213583 and 213621, as well as Mg and SCIg. Human FcRn-eGFP transduced HeLa cells were incubated with human Alexa Fluor® 647 ated lgG. The binding was blocked with unlabeled Z variants, IVIg or SCIg at different trations. The results showed that the FcRn binding dimers have an ed blocking effect in terms of hIgG g to hFcRn compared to the monomeric Z variant 207918, Mg and SCIg (Figure 12A). Furthermore, the blocking capacity of maturated monomeric 2 ts 213583 and 213621 was improved compared to the ng capacity of the primary monomeric 2 variant 207918 (Figure 12B).
The calculated IC50 values of the blocking assay are summarized in Table 15.
Table 15: Calculated IC50 values from HeLa cell IoG blockino assa .
Designation SEQ ID NO: — G4S 3—211948 369 21 1948-(G4S)—Z11948 368 213583 23 Z13621 44 207918 IVIo n.a.
SCI.
Example 15 Production ofdimeric FcRn binding polypeptides Materials and methods The 2 variants 217303 (SEQ ID NO:357), 218632 (SEQ ID NO:365), 218633 (SEQ ID NO:366) and 218634 (SEQ ID NO:367) were constructed as dimers in fusion with the albumin binding variant PP013 (SEQ ID NO:377) in the general format [2#####]—ASGS-PP013-GT-(G4S)—[2#####]. The resulting polypeptides were denoted 2AZ3824 (SEQ ID NO:373), 2AZ3869 (SEQ ID W0 2016;042083 NO:374), ZAZ3870 (SEQ ID NO:375) and ZAZ3871 (SEQ ID NO:376), respectively. Dimeric polypeptides of the Z variant 217303 in fusion with PP013 was also constructed with different linkers or C-terminal fusion of PP013, resulting in the polypeptides Z17303-GAP(G4S)3TS-PP013- GT(G4S)3PR-Z17303 and Z17303-GAP(G4S)3TS-Z17303-GT(G4S)3PR- PP013, denoted ZAZ3715 (SEQ ID NO:371) and ZZA3716 (SEQ ID ), respectively. Furthermore, 217303 was constructed as a dimer without PP013, but with an N—terminal Hise-tag resulting in the polypeptide GSS-Hise- LQ-Z17303-GT(G4S)3-Z17303, denoted 223556 (SEQ ID NO:370).
Cultivation was performed as described in e 3. Purification of PP013 containing polypeptides was carried out by anti-ABD affinity chromatography and RPC as bed in Example 6, s cation of Hiss-tagged 223556 was performed by IMAC as described in Example 3.
Results The seven FcRn binding dimeric polypeptides, constructed either with a Hiss-tag or an ABD moiety, were produced in E. coli. The amount of affinity purified protein, determined spectrophotometrically by measuring the absorbance at 280 nm, ranged from 2-18 mg per g bacterial pellet. SDS- PAGE analysis of each final protein preparation showed that these predominantly contained the FcRn binding polypeptide. The correct ty and molecular weight of each FcRn binding polypeptide was confirmed by HPLC-MS analysis.
Example 16 Binding of homo- and/or heterodimeric FcRn binding ptides to human FcRn/eGFP transfected HeLa cells In this Example, the binding ability of homo- and/or heterodimeric FcRn g polypeptides comprising ted Z ts is investigated. HeLa cells sing human FcRn—eGFP gene transgene, produced as described in Example 4, are used for flow cytometry analysis with Alexa Fluor® 647 labeled Z variants.
W0 2016;042083 Materials and methods Alexa Fluor® 647 labeling of FcRn binding Z variants: Homo- and/or heterodimeric FcRn binding polypeptides are labeled with Alexa Fluor® 647 Carboxylic Acid imidyl Ester (Invitrogen cat. no. A20106) as described in Example 13.
Immunofluorescence staining of human FcRn-eGFP transfected HeLa- cells with FcRn binding polypeptides: hFcRn-eGFP HeLa cells are harvested by trypsination and washed twice in Mcllvanes buffer, pH 6.0, before counting. 100,000 cells are pipetted per well of a v-bottomed 96 well plate (Nunc, cat no 277143) and the cells in the plate are subsequently pelleted at 1,700 rpm for 4 min at 4 °C. The supernatants are removed and the cells are fixed with 50 pl of 2 % formaldehyde (Sigma Aldrich, cat. no. F8775) in Mcllvanes buffer for 10 min at RT. Cells are thereafter washed with 2 x 100 pl Mcllvanes buffer, pH 6.0, containing 2.5 % FBS Ultra low lgG (Life Technologies), and resuspended in Mcllvanes buffer, pH 6.0, containing 2.5 % FBS Ultra low IgG and 0.1 % saponin (AppliChem, cat no A4518.0100) containing 640 nM of Alexa Fluor® 647 labeled Hise-tagged homo- and/or heterodimeric FcRn binding polypeptides and a corresponding monomeric Z variant.
Examples of formats for homo— and/or dimers include Z#####- (G4S)3-Z##### and Z#####-(G4S)-Z#####, where Z#### for example is selected from Z13583 (SEQ ID NO:23), Z13621 (SEQ ID , 213654 (SEQ ID NO:65) or Z13674 (SEQ ID NO:75), or the same Z variants ng with amino acid residues AE d of VD, as for example in Z17303 (SEQ ID NO:357), which is identical to 213621 (SEQ ID NO:44) apart from the N- al AE. Cloning may optionally be performed with a C-terminal Hise tag as described in Example 3 or with an N-terminal Hise tag as in SEQ ID NO:362. uced HeLa cells, incubated with buffer alone, are used as control. The cells are incubated for 1 h at 8 °C on a shaker in the dark. The cells are then subjected to two ent washing conditions; 2 x 150 pl Mcllvanes buffer, pH 6.0, ning 2.5 % FBS Ultra low IgG or 2 x 150 pl PBS, pH 7.4, containing 2.5 % FBS Ultra low IgG and a 20 min incubation step in PBS, pH 7.4, containing 2.5 % FBS Ultra low lgG. After washing, all samples are pended in 180 pl of Mcllvanes, pH 6.0, containing 2.5 % FBS Ultra low lgG. Data from 10,000 Rn positive cells are obtained W0 2016;042083 using a FACS Calibur (Beckman Coulter) and the data is analyzed using g software 2.5.0 (Turku University).
Results Flow cytometry analysis is used to determine whether the homo- and/or dimeric FcRn binding polypeptides comprising maturated Z variants can bind to human FcRn on human FcRn/eGFP transduced HeLa cells and compare the binding ability to the ric FcRn binding Z variants or dimeric variants comprising primary Z variants. The analysis is also performed to determine if the pH dependent detachment from the FcRn protein is affected by the dimeric format. The experiments are performed at pH 6.0 with washings at pH 6.0 or pH 7.4 with Alexa Fluor® 647 labeled FcRn binding Z variants. The results from the experiment are expected to show that homo- and/or dimeric formats, as well as the inclusion of maturated Z variants with an improved affinity for FcRn, increase the binding capacity of the FcRn binding polypeptides and that the pH dependent detachment from FcRn is sed for said polypeptides.
Example 17 pH dependent binding ofdimeric polypeptides to human FcRn In this Example, the capacity ofdimeric polypetides to bind FcRn at different pH values was investigated by ELISA and compared to the g capacity of a monomeric Z variant.
Materials and methods The capacity of the dimeric polypeptides ZA23824 (SEQ ID NO:373), ZA23869 (SEQ ID ), ZAZ3870 (SEQ ID NO:375) and ZAZ3871 (SEQ ID NO:376), as well as the ric Z variant 213621 (SEQ ID NO:44), to bind human FcRn at different pH values was tested in an ELISA where all binding and washing steps were med at either pH 6.0 or pH 7.4. Half- area 96-well ELISA plates were coated at 4 °C overnight with 4 ug/ml of hFcRn (Biorbyt, cat. no. orb84388) d in PBS. The plates were washed twice in tap water and the wells were blocked with 100 pl of PBSC (PBS, pH 7.4, mented with 1 % casein) for 1.5 h at RT. The blocking solution was W0 2016;042083 poured off and the wells subjected to pH 6.0 treatment were washed once with Mcllvaines phosphate-citrate buffer, pH 6.0. The different FcRn binding polypeptides were added at a concentration of 100 nM and diluted stepwise 1:10 down to 0.1 pM in either PCC (Mcllvaines phosphate—citrate buffer, pH 6.0, supplemented with 1% casein) or PBSC. 50 pl of the dilutions were transferred per well and the ELISA plates were incubated for 1.5 h at RT. The plates were washed four times in either PCT (Mcllvaines phosphate-citrate buffer, pH 6.0, supplemented with 0.05 % Tween-20) or PBST (PBS, pH 7.4, mented with 0.05 % Tween-20). Bound polypeptides were detected with 50 ul/well of a Z specific mouse antibody ced in-house) diluted to 2 ug/ml in either PCC or PBSC. The plates were subsequently incubated for 1.5 h at RT ed by washing as described above. HRP-conjugated goat anti- mouse lg obtained from DAKO (P0447), diluted 1:5000 in either PCC or PBSC, was added and the plates were incubated at RT for 1 h. After washing (as above), 50 pl of ImmunoPure TMB substrate was added to each well and the plates were developed according to the manufacturer’s recommendations.
After 30 min of development, the ance was measured at 450 nm using a multi-well plate reader (Victor3) and the EC50 values were calculated using GraphPad Prism 5.
Results The analysis was performed to compare the binding potential of ric versus dimeric format at different pH, but also to determine r introduced scaffold mutations (Y5F, N528 and D53E; see further in Examples 24 and 25) would affect the pH dependent g to FcRn. The experiment was performed in an ELISA format at pH 6.0 or pH 7.4. The results showed that the dimeric format was or to the monomeric format in binding FcRn regardless of pH (up to 10x and 85x improvement at pH 6.0 and pH 7.4, respectively). The most potent binding, both at pH 6.0 and pH 7.4, was seen for the dimeric polypeptide ZAZ3824, which also showed a similar binding capacity (EC50 value) at pH 6.0 and pH 7.4. The ELISA titration curves are shown in Figure 13, and the calculated EC50 values are summarized in Table 16.
W0 2016;042083 Table 16. Calculated E050 values from binding analysis at pH 6.0 and pH 7.4 EC50 (M) DeSIgnation. . SEQ ID NO: pH 6.0 pH 7.4 Z13621 44 9.9 x10'10 7.7 x10'9 ZAZ3824 373 9.9 x 10'11 9.1 x 10'11 ZAZ3869 375 3.0 x 10'10 7.3 x 10-10 ZAZ3870 375 2.6 x 10'10 5.7 x 10‘10 ZAZ3871 376 1.4 x 10'10 1.8 x 10‘10 Example 18 Comparison of ng capacity of lgG binding to FcRn In this e, the potential of ptides to inhibit binding of IgG to FcRn was analyzed using two different in vitro methods: AlphaLlSA and a cell based assay.
Materials and methods lSA blocking assay: The capacity of the dimeric FcRn binding polypeptides ZZ3556 (SEQ ID NO:370), 5 (SEQ ID NO:371) and ZZA3716 (SEQ ID NO:372) as well as the ric Z13621 (SEQ ID NO:44) to block lgG-FcRn interaction was ed using an AlphaLlSA assay. Human lgG (Roactemra) was immobilized on AlphaLlSA acceptor beads (Perkin Elmer, cat. no. 6772002) according to the manufacurer’s recommendations. Human FcRn (Biorbyt, cat. no. orb84388) was biotinylated essentially as described in Example 2. Polypeptides were serially diluted 1:3 in AlphaLlSA buffer (Perkin Elmer, cat. no. AL000F) pH 6.0 (adjusted using HCI) to final concentrations of 250 nM to 13 pM in a 384—well plate (Perkin Elmer, cat. no. G6005350) and incubated for 45 min with 10 nM biotinylated hFcRn. lgG-coated acceptor beads were added to a final concentration of 10 pg/ml and incubated for 45 min. Finally, streptavidin coated donor beads (Perkin Elmer, cat. no. 6760002) were added to a final tration of 40 ug/ml and incubated for 30 min. All incubations were performed at RT in the dark. The plate was analyzed in the EnSpire multiplate reader 2300 (Perkin Elmer) and the |C50 values were calculated using GraphPad Prism 5.
HeLa cell IgG-FcRn blocking assay: Human FcRn-eGFP transduced HeLa cells were prepared as described in Example 4. The polypeptides 223556 (SEQ ID ), 2A23715 (SEQ ID NO:371), 22A3716 (SEQ ID ), ZAZ3824 (SEQ ID NO:373), 2A23869 (SEQ ID NO:374), 2A23870 (SEQ ID NO:375), 2A23871 (SEQ ID NO:376), 213621 (SEQ ID NO:44), 218632 (SEQ ID NO:365), 218633 (SEQ ID NO:366) and 218634 (SEQ ID NO:367), as well as IVlg (Octagam®, Octapharma) or SClg (Gammanorm®, Octapharma), were each diluted to concentrations of 1000, 100, 10, 1, 0.1 or 0 (buffer control) nM in Mcllvanes buffer, pH 6.0, containing 2.5 % FBS Ultra low IgG (Life Technologies) and 0.1 % saponin (AppliChem) and 50 nM Alexa Fluor® 647-conjugated human IgG (Jackson laboratories, cat. no. 009 003). Fixed cells were resuspended in 100 pl of the mixture and were incubated for 1 h at 37 °C in the dark. Cells were washed and resuspended in Mcllvanes buffer, pH 6.0, containing 2.5 % FBS Ultra low IgG. Data from 10,000 GFP/FcRn positive cells was obtained using a FACS Calibur (Beckman Coulter), analyzed using Flowing re 2.5.0 (Turku University) and IC50 values were calculated using GraphPad Prism 5.
Results ISA: The y of one monomeric and three dimeric polypeptides to inhibit IgG binding to FcRn was tested in an AlphaLISA blocking assay. The s show that the dimeric polypeptides had better IgG blocking capacity compared to the monomeric format. The two ABD fused dimeric ptides, 2A23715 and 22A3716, had IC50 values very similar to that of , which does not contain an ABD . The calculated IC50 values of the AlphaLISA blocking assay are summarized in Table 17.
HeLa cell IgG blocking assay: Human FcRn-eGFP transduced HeLa cells were incubated with human Alexa Fluor 647-conjugated IgG and selected polypeptides to assess the ability of the polypeptides to block IgG- FcRn interactions. Intravenous immunoglobulin (Mg) and aneous immunoglobulin (Sclg) currently used in the treatment of some autoimmune disorders, were also included in the test. The experiment was performed at pH 6.0. The results showed that the dimeric format had an ed IgG blocking capacity compared to the monomeric format. 2A23715 (longer linkers) was compared to ZAZ3824 (shorter linkers), and the two constructs showed similar IC50 values. There was also no difference between IC50 values ed for 2A23715 and 22A3716, i.e. dimer constructs with ABD at WO 2016042083 different positions. All tested ptides had superior lgG blocking effect compared to Mg and SCIg. The ated |C50 values of the cell blocking assay are summarized in Table 18.
Table 17: Calculated |C50 values showing lgG blocking capacity of polypeptides in lSA.
Designation ‘ SEQ ID NO: |C50 (M) 213621 ‘ 44 1.0 x10'9 223556 370 1.9 x 10'10 2A23715 371 1.7 x10'10 22A3716 ‘ 372 1.8 x 10'10 Table 18. Calculated |C50 values showing lgG blocking capacity of polypeptides in a HeLa cell based assay at pH 6.0.
Designation SEQ ID NO: |C50 (M) 213621 44 2.2 x10'8 218632 365 8.4 x 10'8 218633 366 5.7 x 10'8 218634 367 3.6 x 10'8 223556 370 1.9 x 10'9 2A23715 371 3.6 x 10'9 22A3716 372 3.5 x 10'9 ZAZ3824 373 3.8 x 10'9 2A23869 374 4.7 x 10'9 2A23870 375 5.3 x 10'9 2A23871 376 4.1 x 10'9 SClg - 1.2 x 10'7 Mg - 1.5 x 10'7 W0 2016;042083 Example 19 Comparison of blocking ty of IgG recycling In this Example, the effect of homo- and/or heterodimeric FcRn g polypeptides on lgG ing are investigated in FcRn transduced MDCK.2 cells.
Materials and methods Lentiviral transduction of MDCK.2 cells: The vector pairs 2k7neo-CMV- hB2M and pHR—cPPT-CMV-hFcRn-eGFP are nsfected together with VSV-G pe and gag/pol packaging plasmid into HEK293T cells using calcium chloride transfection (Zufferey et al., supra; son etal. (2006) supra). HEK293T culture supernatants containing formed lentiviral particles with FcRn and 82M transgenes respectively are used to sequentially transduce MDCK.2 cells (ATCC cat. no. CRL—2936) at low passage number.
The resulting stably transduced MDCK.2 cell lines are denoted hFcRn-eGFP (transduced with genes for human FcRn-eGFP and hB2M). ng capacity of lgG recycling: Human GFP transduced MDCK.2 cells are plated at 25 000 cells/well in a 96—well plate and incubated overnight at 37 °C, 5 % C02. The cells are incubated with homo— and/or heterodimeric FcRn binding polypeptides at concentrations ranging from 200 to 0.01 nM in Mcllvanes buffer, pH 6.0, containing 2.5 % FBS Ultra low lgG before addition of 500 ng/ml Alexa Fluor® 647-conjugated human lgG (Jackson laboratories, cat. no. 009003) and incubation for one additional hour. The cells are washed Mcllvanes buffer, pH 6.0, ning 2.5 % FBS Ultra low lgG and then incubated in PBS, pH 7.4, containing 2.5 % FBS Ultra low lgG for 2 h in 37 °C, 5 % C02. The supernatants are then analyzed for the presence of the Alexa Fluor® 647-conjugated human lgG in an EnSpire multiplate reader (Perkin Elmer). The inhibition curves are analyzed by non- linear regression using the GraphPad Prism 5 software to determine the |C50 values.
Results The results from the experiment are expected to show a dose dependent reduction in lgG recycling through the action of dimeric FcRn binding polypeptides.
W0 2016;042083 Example 20 Increased lgG catabolism by dimeric FcRn binding polypeptides in FcRn transgenic mice In this Example, the effect of homodimeric FcRn binding polypeptides on lgG catabolism was investigated in human FcRn transgenic mice on two different occasions.
Materials and methods Animal studies: In the first study, homodimeric FcRn-binding polypeptide ZAZ3715 (SEQ ID NO:371) or ZZA3716 (SEQ ID NO:372), or vehicle (PBS buffer), was administered at 0 hours by iv ion to male BS.Cg-chrttm1Dcr Tg(FCGRT)32Dcr/Dch mice (Jackson Laboratory, stock no. 14565) at a dose of 6 mg/kg. 24 hours prior to polypeptide administration, 500 mg/kg hlgG (Kiovig, Baxter) was administered by iv injection. Serum samples were collected at -168, 0, 24, 72, 120 and 144 h (termination of study) and stored at -20 °C.
In the second study, homodimeric inding polypeptide ZAZ3869 (SEQ ID NO:374), ZAZ3870 (SEQ ID NO:375) or ZAZ3871 (SEQ ID NO:376), or vehicle (PBS buffer), was stered at 0 hours by i.v. injection to male B6.Cg-chrttm1Dcr RT)32Dcr/Dch mice (Jackson Laboratory, stock no. 14565) at a dose of 6 mg/kg. 24 hours prior to ptide administration, 500 mg/kg hlgG (Kiovig, Baxter) was administered by iv injection. Serum samples were collected at -168, 0, 24, 72, 120 and 144 h nation of study) and stored at -20 °C.
Human lgG ELISA: The concentration of human lgG in mouse serum samples collected from the two studies was analyzed with a human lgG AlphaLlSA kit (Perkin Elmer, cat. no. AL2050) as described by the manufacturer. The concentration of hlgG was calculated from a standard curve and GraphPad Prism 5 using a non-linear regression formula.
Results The s from the experiments show a ion in the concentrations of human lgG over time through the action of the FcRn binding polypeptides. There were little or no difference in the reduction of lgG levels W0 2016;042083 between the two groups receiving the polypeptides ZAZ3715 and 6 (Figure 14A). These results indicate that FcRn binding polypeptides with a central or C-terminal positioning of ABD are equally ent in sing lgG catabolism. The polypeptides ZAZ3869, ZAZ3870 and ZAZ3871, respectively, reduced hlgG levels to a similar extent (Figure 148). The results from the second ment were in the same range as the results obtained in the first experiment.
Thus, the results from the two experiments te that the FcRn- specific ptides blocked recycling of IgG, resulting in increased lgG catabolism and subsequent lower levels of IgG in human FcRn transgenic mice.
Example 21 Increased lgG catabolism by dimeric FcRn binding polypeptides in NMRI mice In this Example, the effect of homodimeric FcRn binding polypeptides on lgG catabolism was investigated in NMRI mice. als and methods Animal study: Homodimeric FcRn-binding polypeptide ZAZ3715 (SEQ ID NO:371) or 4 (SEQ ID N02373), or vehicle (PBS buffer), was administered at 0 hours by iv. injection to female NMRI mice, at a dose of 0.6 or 1.7 umol/kg. Serum samples were collected at 0, 24, 48 and 72 h (termination of study) and stored at -20 °C.
Mouse IgG ELISA: The concentration of mouse lgG in mouse serum samples was ed by a mouse IgG ELISA kit (Mabtech, . 3825- 1AD-6) and performed as described by the manufacturer. The concentration of mlgG was calculated from a standard curve and GraphPad Prism 5 using a non-linear regression formula. The concentration of lgG in individual mice at 24, 48 and 72 h were related to the level at 0 h and the results are therefore presented as percentage of lgG (0 h).
PK ELISA: Concentrations of FcRn-binding polypeptides in mouse serum samples were determined by ELISA. In this assay, 96-well half area plates were coated with a mouse anti Z polyclonal antibody (produced in- house) at a concentration of 4 ug/ml in PBS (50 ul/well) and incubated overnight at 4 °C. Next, the plates were rinsed twice in tap water and blocked W0 2016;042083 with ng buffer for 1 hour. An in-house Z variant standard was titrated in a 3-fold dilution series —300 ng/ml) and d serum samples were added to the coated ELISA plates (50 ul/well) and incubated for 1.5 h at RT.
The plates were washed 4 times in an automated ELISA washer and 4 ug/ml (50 l) of a goat anti-ABD polyclonal antibody (produced in-house) was added. After incubation for 1 h, the plates were washed and 50 ul of anti-goat lgG-HRP on, cat. no. 711152) at a concentration of 20 ng/ml was added to each well. After one additional hour of incubation and subsequent washing, the plates were developed with 50 ul TMB per well and the reactions were stopped with 50 pl 2M H2804. The absorbance at 450 nm was measured in a 96-well plate reader (Victor3).
Results lgG catabolism: The results showed a reduction of mouse lgG concentration in mice treated with the FcRn-specific polypeptides ZAZ3715 and ZAZ3824, respectively (Figure 15). The result obtained with polypeptide ZAZ3824 shows that the reduction in endogenous lgG levels is dose dependent, and the most pronounced effect was observed at 72 h. Notably, the reduction of lgG levels obtained with the use of dimers was greater than the ion ed with monomeric 207918 in e 11, even though the monomer was administrated repeatedly and at much higher dose than the dimers re Figures 15 and 10 at 72 h). Thus, the results indicate that the FcRn-specific polypeptides disclosed herein could block recycling of lgG, resulting in an increased lgG catabolism and subsequent lower levels of lgG in mice.
Pharmacokinetic analysis: The pharmacokinetic profiles of ZAZ371 5 and ZAZ3824 are shown in Figure 16. The half-life of ZAZ3824 was approximately 62 hours, which is in line with the half-life obtained in the pharmacokinetic study presented in Example 6, demonstrating that the cokinetic properties of the polypeptides are further improved by binding to FcRn in addition to the prolonged half-life resulting from albumin binding.
W0 2016;042083 e 22 Increased IgG catabolism by FcRn binding dimers in cynomolgus monkeys In this Example, the effect of homo- or heterodimeric FcRn binding polypeptides on IgG catabolism is investigated in cynomolgus monkeys.
Materials and s Animal study: Homo- and/or heterodimeric FcRn binding polypeptides recombinantly fused to PP013 (SEQ ID NO:377) are ed. Examples of such FcRn binding dimers include SEQ ID NO:371-376 disclosed herein.
The FcRn binding dimers including an albumin binding domain are then administered to cynomolgus monkeys at a dose of 2, 0.4 and 0.1 pmol/kg. The dimeric polypeptides are injected intravenously at 0 hours. The monkeys are bled 1 h prior to the first administration and daily up to day 21, and then once a week up to day 50. Sera are prepared and stored at -20 °C.
The concentration of cynomolgus IgG is determined by ELISA.
Homo- and/or heterodimeric FcRn binding polypeptides without an albumin binding domain are administered to lgus monkeys at a dose of 2, 0.4 and 0.1 pmol/kg. The dimeric polypeptides are administered three times per week for two weeks. The monkeys are bled 1 h prior to the first administration and daily up to day 21, and then once a week up to day 50.
Sera are prepared and stored at -20 °C. The concentration of cynomolgus IgG is determined by ELISA.
Cynomolgus IgG ELISA: The tration of cynomolgus IgG in serum samples is determined with a human/cynomolgus IgG ELISA kit (for e Mabtech 3850-1AD-6, which is reactive with both human and cynomolgus IgG) as described by the cturer.
Results The results from the experiment are expected to show a dose dependent reduction in the concentrations of cynomolgus IgG through the action of FcRn binding dimers, with a more pronounced effect for such dimers that also se an albumin binding domain.
W0 2016;042083 Example 23 Pharmacokinetic study ofdimeric FcRn binding ptides In this Example, the serum half-life of homo- and/or heterodimeric FcRn binding polypeptides is investigated in a pharmacokinetic study performed in mice.
Materials and methods Pharmacokinetic study: FcRn binding polypeptides, as homo- and/or heterodimers alone or recombinantly fused to PPO13 (SEQ ID NO:377) are administered intravenously (i.v.) to male NMRI mice (Charles River, Germany) at a dose of 92 nmol/. Sera from groups of three mice are obtained at 0.08, 6, 18, 78, 120, 168 and 240 hours. The concentration of respective polypeptide is determined by ELISA.
ELISA: rea 96-well ELISA plates are coated at 4 °C overnight with 50 ul/well of a goat antibody ic for Z variants in general (produced in-house) diluted to 4 ug/ml in coating buffer (50 mM sodium carbonate, pH 9.6). The antibody solution is poured off and the wells are blocked with 100 pl of PBSC for 1.5 h at RT. The sera are diluted in PBSC containing 1 % mouse serum (matrix) from 1:100 to 1:51 ,200 in a two-fold on series in a dilutions plate. A standard titration for respective Z variant polypeptide and four quality ls (very low, low, medium and high control) diluted in matrix are included on each plate. 50 ul of the dilutions are transferred per well and the ELISA plates are incubated for 1.5 h at RT. The plates are washed four times with PBST. Bound Z variant polypeptides are detected with 50 l of rabbit PO13 lg (produced in-house) or 50 ul/well of mouse anti-Z mAb (produced in-house) diluted to 4 ug/ml in PBSC. The plates are subsequently incubated for 1.5 h at RT followed by washes as described above. HRP conjugated donkey anti-rabbit HRP (Jackson laboratories; cat. no. 711 152), diluted 120,000 in PBSC, is added and the plates are ted for 1 h.
After washing as described above, 50 ul of ImmunoPure TMB ate is added to the wells and the plates are developed according to the manufacturer’s recommendations. After 15 min of development, the absorbance is measured at 450 nm using a multi-well plate reader (Victor3).
The absorbance values are analyzed using GraphPad Prism 5 to determine the trations —spline curve fit) and area under curve (AUC). The concentrations are then plotted as their natural logarithms against time. The W0 2016;042083 resulting curves are ed to follow a two compartment model and the al half-life is calculated as ln2 divided by the slope based on the last three time points.
Results The results from the experiment described herein are expected to show a two compartment elimination phase with a terminal half-life of approximately 60 min for ptides without ABD and approximately 90 hours for polypeptides comprising ABD.
Example 24 Generation, stability study and binding assessment of scaffold-modified FcRn binding polypeptides The following Example discloses ld modified FcRn binding Z variants exhibiting improved stability at elevated temperatures. The Z variants 217347 (SEQ ID NO:358), with the amino acid tutions N528 and D53E, and 217348 (SEQ ID NO:359), with the amino acid substitutions D36R, D37Q, S39E, N528 and D53E, are compared to their parent le 211948 (SEQ ID NO:354) in terms of stability and binding capacity to FcRn.
Materials and methods Generation of ld—modified polypeptides: 217347 (SEQ ID NO:358), 217348 (SEQ ID NO:359) and 211948 (SEQ ID NO:354) were cloned with an N-terminal 6 x ine-tag (Hisa) and obtained constructs encoded polypeptides in the format MGSSHHHHHHLQ-[2#####]. Mutations were introduced in the plasmids of the modified 2 ts using overlapping oligonucleotide primer pairs encoding the desired amino acid substitutions and by applying established molecular biology techniques. The correct plasmid sequences were verified by DNA sequencing.
E coli (strain T7E2) cells (GeneBridge) were transformed with plasmids containing the gene fragments ng the original and scaffold modified 2 variants. The cells were cultivated at 37 °C in TSB-YE medium supplemented with 50 pg/ml kanamycin and protein expression was subsequently induced by addition of IPTG. Pelleted cells were disrupted using a FastPrep®-24 homogenizer (Nordic Biolabs) and cell debris was removed by centrifugation.
W0 2016;042083 Each supernatant containing the Z variant as a Hise—tagged protein was purified by immobilized metal ion affinity chromatography (IMAC) using His GraviTrapTM columns (GE Healthcare) according to the manufacturers instructions. Purified Z variants were buffer exchanged to phosphate-buffered saline (PBS; 1.47 mM KH2PO4, 8.1 mM NagHPO4, 137 mM NaCl, 2.68 mM KCI, pH 7.4) using PD—10 desalting columns (GE Healthcare). The correct identity of each polypeptide was verified by GE and HPLC-MS. ar dichroism oscopy analysis: Circular dichroism (CD) analysis was carried out as described in Example 3 to determine the melting temperatures (Tm) and assess potential changes in the secondary structure of the inventive polypeptides as a result of the amino acid substitutions.
Comparative ity study: The Hise-tagged Z variants, formulated in PBS pH 7.4, were diluted to 1 mg/ml and 200 pl aliquotes were incubated at 37 °C for 2 weeks. Samples collected prior to and after the stability test were ed by SDS-PAGE using 10% Bis-Tris NuPAGE gels (lnvitrogen) and by g 5 pg protein into each well. The stabilty was assessed by the appearance of new variants after incubation at the elevated temperature and mutated variants were compared to the original polypeptide.
Binding assessment of scaffold-modified ptides: The Hiss- tagged Z variants were further assessed in terms of ved binding capacitiy to FcRn after introduction of alterations in the scaffold, as well as after having been ted to the stability test, i.e. incubated at 37 °C for 2 weeks. Comparative kinetic constants (kon and koff) and affinities (KD) were determined using a Biacore 2000 instrument. The target protein human FcRn (Biorbyt, cat. no. orb 84388) was immobilized on the carboxylated dextran layer surface of a CM5 chip (GE Healthcare). The immobilization was performed using amine coupling chemistry according to the cturer’s protocol and using HBS-EP as running buffer. One flow cell surface on the chip was activated and deactivated for use as blank during analyte ions.
The immobilization level of hFcRn on the surface was approximately 750 RU.
The Z variants were diluted in running buffer to final concentrations of 3.33, and 30 nM and injected for 3 min, followed by 15 min of dissociation in running . Regeneration by three pulses of HBS—EP ed by 10 min equilibration in running buffer was applied after each analyte injection. c constants were calculated from the sensorgrams using the Langmuir 1:1 model of the BiaEvaluation software 4.1 (GE Healthcare). Curves of the blank surface were subtracted from the curves of the ligand surfaces and the data W0 2016;042083 from the buffer cycles were subtracted from the data of the ample cycles to correct for any drift in signal.
Results Circular dichroism spectroscopy analysis: The Tm of each respective Z variant as determined from the midpoint of the transition in the CD signal vs. temperature plot is shown in Table 19. The mutated Z variants showed preserved alphahelical structure and refolded reversibly after heating to 90 °C.
Table 19: Melting temperatures for original and mutated Z variants.
Designation SEQ ID NO Tm (°C) Original vs modified Z11948 354 48 Original 217347 358 50 Modified Z17348 359 44 Modified Comparative stability study: The d modified Z variants 217347 and 217348 showed an improved stability compared to the al polypeptide 211948. The second band visible on the gel just above the main band for 211948 was not visible in s of 217347 and 217348 (Figure 17), Le. the scaffold mutations prevent the formation of the alternative s observed for the sample in the original scaffold.
Binding assessment of scaffold-modified polypeptides: The ative kinetic constants for the FcRn-binding Z variants are shown in Table 20. The affinity was marginally effected by the amino acid substitutions ND to SE in position 52-53, such as in 217347 (SEQ ID NO:358), as well as by the substitutions ND to SE in position 52-53 in combination with D36R, D37Q and S39E, such as in 217348 (SEQ ID NO:359), and onal binders were obtained with KD in the range of 10'9 M. The assessed variants also had preserved binding capabilities after 2 weeks incubation at 37 °C.
WO 2016042083 Table 20. Comparative kinetic analysis of original and modified Z variants.
Orisgaffoldinal vs Test sample SEEDFD ka (Ms'1) k,| (5'1) KD (M)* ' modified 211948 (0) 354 Original 1.80x106 4.56x10'3 2.9x10'9 211948 (2w) 354 Original 3.15x106 5.75 x10'3 1.8x10'9 217347 0 358 Modified 1.18x106 7.99 x10'3 6.7x10'9 217347 (2w) 358 Modified 2.27x10 8.79 x10‘ 3.9x10' 217348 (0) 359 Modified 1.82x106 1.00 x10'2 5.5x10'9 217348 (2w) 359 Modified 1.28x106 8.09 x10'3 6.3x10'9 * The KD values should not be regarded as absolute, as these were determined for comparative purposes and only included a limited number of sample concentrations.
Example 25 Generation and assessment of onal scaffold-modified FcRn binding ptides In this e, additional variants with the scaffold amino acid substitutions N528 and D53E, and some also with the amino acid substitution Y5F, were analyzed in terms of their stability and binding to FcRn as compared to their respective parent FcRn binding Z variant. The results show that the structure, stability and FcRn binding capacity are retained in the mutated variants.
Materials and methods Generation of scaffold-modified polypeptides: The amino acid substitutions N528 and D53E were introduced in the plasmids of Hise-tagged Z13578 (SEQ ID , 213583 (SEQ ID NO:23), 213616 (SEQ ID NO:41), 213621 (SEQ ID NO:44) and 213674 (SEQ ID NO:75) using established molecular biology ques resulting in the respective Z variants 218614 (SEQ ID NO:360), 218615 (SEQ ID NO:361), 218616 (SEQ ID NO:362), 218617 (SEQ ID NO:363) and 218618 (SEQ ID NO:364). Additional modifications at position 5, were the tyrosine residue was substituted to alanine, as well as N-terminal cation to start with the amino acid residues AE instead of VD, resulted in the 2 variants 218632 (SEQ ID ), 218633 (SEQ ID NO:366) and 218634 (SEQ ID NO:367) having g motifs (BM) cal to 213578, 213616 and 213621, respectively.
Cultivation and purification was performed essentially as described in W0 2016;042083 Example 3 and e 9. The five Z ts 218614-218618 were purified by IMAC only, whereas the three Z variants 218632-218634 were further purified by RPC.
CD is: CD analysis was d out as bed in Example 3 to determine the melting temperatures (Tm) and assess potential changes in the secondary structure of the d Z ts compared to their respective parent Z variant.
Biacore binding analysis: A binding analysis at pH 6.0, using a Biacore instrument, was performed essentially as described in Example 3. A concentration series (270, 90, 30, 10 and 3.3 nM) of the Hiss-tagged Z variants 218632, 218633 and 218634 and their respective corresponding parent Z variants 213578, 213616 and 213621, were injected during 4 min at ul/min over hFcRn yt, cat. no. orb84388) and chRn (Biorbyt, cat.no. orb99075), immobilized in different flow cells of a CM5 chip surface. 0.005 % PCT pH 6.0 was used as running buffer and for dilutions of the Hise-tagged 2 variants. Dissociation in running buffer was allowed for 20 min, followed by surface regeneration by injection of 3 x 30 second pulses of 0.00 5% PCT pH 7.4 and equilibration for 15 min before the start of next cycle.
Results Cultivation and cation: The FcRn g 2 variants were constructed with an N—terminal Hiss—tag and produced in E. coli. SDS—PAGE analysis of each final protein preparation showed that it predominantly contained the 2 variant. The correct identity and molecular weight of each FcRn g 2 variant was confirmed by HPLC-MS analysis.
CD analysis: The Tm of the mutated FcRn binding 2 variants were identical, or nearly identical, to the Tm of the respective parent 2 variant (Table 21). Furthermore, reversible g was observed for all seven 2 variants by overlaying a obtained before and after heating to 90 °C.
Biacore g analysis: The binding profiles for interactions with FcRn at pH 6.0 were compared pairwise for three mutated 2 variants and their respective parent 2 variants; 218632/213578 (SEQ ID NO:365/20), 218633/213616 (SEQ ID NO:366/41) and 218634/213621 (SEQ ID NO:367/44). Overlays of sensorgrams from the 90 nM injections of the Z variants over hFcRn and chRn surfaces show that the mutated 2 variants retained their ability to bind to human and cynomolgus FcRn (Figure 18A—C).
The FcRn immobilization levels of the chip surfaces were 1577 RU for human FcRn and 1098 RU for cynomolgus FcRn.
Table 21: Meltino temperatures for scaffold mutated and parent Z ts.
—--WZvariant NO: Z variant 218616 362 62 ——m- 218618 364 so 218632 365 56 218633 366 61 m- 218634 367 48 213621 44 49 W0 2016;042083 ITEMIZED LISTING OF EMBODIMENTS 1. FcRn binding dimer, comprising a first monomer unit, a second monomer unit and an amino acid linker, wherein said first and second monomer unit each comprises an FcRn binding motif (BM), which motif consists of the amino acid sequence EX2 X3 X4 AXe X7 EIR 16X17 X18 QR X21 AFIX25 X26LX28 X29 wherein, independently from each other, X2 is selected from A, D, E, F, H, I, K, L, N, Q, R, S, T, V, W and Y; X3 is selected from A, D, E, F, G, H, l, K, L, M, N, Q, R, S, T, V, W and X4 is selected from A, D, E, F, G, H, l, K, L, N, Q, R, S, T, V, W and Y; X5 is selected from A, E, F, G, H, l, K, Q, R, S and V; X7 is selected from A, F, H, K, N, Q, R, S and V; X16 is selected from N and T; X17 is ed from F, W and Y; X18 is selected from A, D, E and N; X21 is selected from A, S, V and W; X25 is selected from D, E, G, H, l, K, L, N, Q, R, S, T, V, W and Y; X26 is selected from K and S; X28 is selected from A, D, E, F, H, l, K, L, N, Q, R, S, T, V, W and Y; and X29 is selected from D and R, and wherein said FcRn binding dimer binds FcRn with a higher g capacity compared to said first monomer unit or said second monomer unit alone. 2. FcRn binding dimer according to item 1, wherein, ndently from each other, X2 is selected from A, D, E, F, H, l, K, L, N, Q, R, S, T, V, W and Y; X3 is selected from A, D, E, F, H, l, K, L, M, N, Q, R, S, T, V, W and Y; X4 is selected from A, D, E, F, H, l, K, L, N, Q, R, S, T, V, W and Y; W0 2016;042083 X5 is selected from A, E, F, G, H, I, K, Q, R and S; X7 is selected from A, F, H, K, N, Q, R, S and V; X16 is selected from N and T; X17 is selected from F and Y; X18 iS D; X21 is V; X25 is selected from D, E, H, l, K, L, N, Q, R, S, T, V, W and Y; X26 is selected from K and S; X28 is selected from A, D, E, F, H, l, K, L, N, Q, R, S, T, V and W; and X29 is selected from D and R. 3. FcRn binding dimer according to item 1, wherein the BM of at least one of said first and second monomer units consists of an amino acid sequence selected from i) EX2 X3 X4 AXe HEIR WLPNLTX17 X18 QR X21 AFIX25 KLX28 D wherein, ndently from each other, X2 is selected from A, D, E, F, H, l, K, L, N, Q, R, S, T, V, W and Y; X3 is selected from A, D, E, G, H, K, L, M, N, Q, R, S, T, V and Y; X4 is selected from A, D, E, F, G, l, K, L, N, Q, R, S, T, V and Y; X6 is selected from A, G, K, R, S and V; X17 is selected from F, W and Y; X18 is selected from A, D, E and N; X21 is selected from A, S, V and W; X25 is selected from D, G, H, K, L, N, R, V and W; X28 is selected from A, D, E, H, K, L, N, Q, R, S, T, W and Y; and ii) an amino acid ce which has at least 96 % ty to a sequence defined by i). 4. FcRn binding dimer according to any one of items 1-3, wherein X6X7 is selected from AH and GH in at least one of said first and second monomer units.
W0 2016;042083 . FcRn binding dimer ing to item 4, wherein X6X7 is AH in at least one of said first and second monomer units. 6. FcRn binding dimer according to item 4, wherein X6X7 is GH in at least one of said first and second r units. 7. FcRn binding dimer according to any preceding item, wherein X17X18 is selected from FD and YD in at least one of said first and second monomer units. 8. FcRn binding dimer ing to item 7, wherein X17X18 is FD in at least one of said first and second monomer units. 9. FcRn binding dimer according to any preceding item, wherein the sequence of the BM of at least one of said first and second monomer units fulfills at least three of the six conditions l-Vl: l. X6 is selected from A, G, K and S, such as in ular A; ll. X7 is H; lll. X17 is selected from F and Y, such as in particular F; IV. X18 iS D; V. X21 is selected from V and W, such as in particular V; VI. X25 is selected from H and R, such as in particular H.
. FcRn binding dimer according to item 9, wherein the sequence fulfills at least four of the six conditions l-Vl. 11. FcRn binding dimer according to item 10, wherein the ce fulfills at least five of the six conditions l-Vl. 12. FcRn binding dimer according to item 11, wherein the sequence fulfills all of the six conditions l-Vl. 13. FcRn binding dimer ing to any preceding item, n said first and second monomer units comprise identical BM sequences. 14. FcRn binding dimer according to any one of items 1-12, wherein said first and second monomer units comprise different BM sequences.
. FcRn binding dimer according to any preceding item, wherein at least one of said first and second monomer units comprises an FcRn binding motif BM corresponding to the sequence from position 8 to position 36 in a sequence selected from the group consisting of SEQ ID NO:1-353, such as the group ting of SEQ ID NO:17-352. 16. FcRn binding dimer according to item 15, wherein at least one of said first and second monomer units comprises a BM ponding to the sequence from position 8 to position 36 in a sequence selected from the group consisting of SEQ ID NO:1-15, SEQ ID NO:17-140 and SEQ ID NO:353. 17. FcRn binding dimer according to item 16, wherein at least one of said first and second monomer units comprises a BM corresponding to the sequence from position 8 to position 36 in a sequence selected from the group consisting of SEQ ID NO:1-2 and SEQ ID NO:17-140, such as the group consisting of SEQ ID NO:17-140. 18. FcRn binding dimer according to item 17, wherein at least one of said first and second monomer units comprises a BM corresponding to the sequence from position 8 to position 36 in a sequence selected from the group ting of SEQ ID NO:1-2, SEQ ID NO:17-92, SEQ ID NO:94-103, SEQ ID NO:105-125 and SEQ ID NO:127-140, such as the group consisting of SEQ ID NO:17-92, SEQ ID NO:94-103, SEQ ID NO:105-125 and SEQ ID NO:127-140. 19. FcRn binding dimer ing to item 16, wherein at least one of said first and second r units comprises a BM corresponding to the sequence from position 8 to position 36 in a ce ed from the group consisting of SEQ ID NO:1-8, SEQ ID NO:13, SEQ ID NO:19-20, SEQ ID NO:23, SEQ ID NO:28, SEQ ID NO:41, SEQ ID NO:44, SEQ ID NO:65, SEQ ID NO:70, SEQ ID NO:73, SEQ ID NO:75-77 and SEQ ID NO:353, such as the group consisting of SEQ ID NO:19-20, SEQ ID NO:23, SEQ ID NO:28, SEQ ID NO:41, SEQ ID NO:44, SEQ ID NO:65, SEQ ID NO:70, SEQ ID NO:73 and SEQ ID NO:75-77.
. FcRn binding dimer according to item 18 or 19, wherein at least one of said first and second monomer units comprises a BM corresponding to the sequence from position 8 to position 36 in a sequence ed from the group consisting of SEQ ID NO:1, SEQ ID NO:20, SEQ ID NO:23, SEQ ID NO:28, SEQ ID NO:41, SEQ ID NO:44, SEQ ID NO:65, SEQ ID NO:73 and SEQ ID NO:75-77, such as the group consisting of SEQ ID NO:20, SEQ ID NO:23, SEQ ID NO:28, SEQ ID NO:41, SEQ ID NO:44, SEQ ID NO:65, SEQ ID NO:73 and SEQ ID 77. 21. FcRn binding dimer according to item 20, wherein at least one of said first and second monomer units comprises a BM corresponding to the sequence from on 8 to position 36 in a sequence selected from the group consisting of SEQ ID NO:1, SEQ ID NO:20, SEQ ID NO:23, SEQ ID NO:41, SEQ ID NO:44, SEQ ID NO:65, SEQ ID NO:75 and SEQ ID NO:77, such as the group consisting of SEQ ID NO:20, SEQ ID NO:23, SEQ ID NO:41, SEQ ID NO:44, SEQ ID NO:65, SEQ ID NO:75 and SEQ ID NO:77. 22. FcRn binding dimer according to item 21, n at least one of said first and second monomer units ses a BM corresponding to the sequence from position 8 to position 36 in a ce selected from the group consisting of SEQ ID NO:1, SEQ ID NO:20, SEQ ID NO:23, SEQ ID NO:41, SEQ ID NO:44, SEQ ID NO:65 and SEQ ID NO:75, such as the group consisting of SEQ ID NO:20, SEQ ID NO:23, SEQ ID NO:41, SEQ ID NO:44, SEQ ID NO:65 and SEQ ID NO:75. 23. FcRn binding dimer according to item 22, wherein at least one of said first and second monomer units comprises a BM corresponding to the sequence from position 8 to position 36 in a sequence selected from the group consisting of SEQ ID NO:1, SEQ ID N023 and SEQ ID NO:75, such as the group consisting of SEQ ID N023 and SEQ ID NO:75. 24. FcRn binding dimer according to item 22, wherein at least one of said first and second monomer units comprises a BM corresponding to the sequence from position 8 to position 36 in a sequence selected from the group consisting of SEQ ID NO:20, SEQ ID NO:41 and SEQ ID NO:44, such as the group consisting of SEQ ID N020 and SEQ ID NO:41; the group consisting of SEQ ID N020 and SEQ ID NO:44; or the group ting of SEQ ID NO:41 and SEQ ID NO:44.
. FcRn binding dimer according to item 22, n at least one of said first and second monomer units comprises a BM corresponding to the sequence from position 8 to position 36 in a ce selected from the group consisting of SEQ ID NO:1, SEQ ID N023 and SEQ ID NO:44, such as the group consisting of SEQ ID N023 and SEQ ID NO:44. 26. FcRn g dimer ing to item 24 or 25, wherein at least one of said first and second monomer units comprises a BM corresponding to the sequence from position 8 to position 36 in sequence SEQ ID NO:44. 27. FcRn binding dimer according to any preceding item, n both said first and second monomer units independently comprise a BM corresponding to the sequence from position 8 to position 36 in a sequence selected as defined in any one of items 15-26. 28. FcRn binding dimer according to item 27, wherein both said first and second monomer units independently comprise a BM corresponding to the sequence from position 8 to position 36 in a sequence selected from the group consisting of SEQ ID NO:20, SEQ ID NO:23, SEQ ID NO:41, SEQ ID W0 2016;042083 NO:44 and SEQ ID NO:75, such as the group consisting of SEQ ID NO:20, SEQ ID NO:41 and SEQ ID NO:44. 29. FcRn binding dimer according to item 28, wherein both said first and second monomer units comprise a BM corresponding to the sequence from position 8 to position 36 in SEQ ID NO:44.
. FcRn binding dimer according to any preceding item, wherein said FcRn binding motif BM in at least one of said first and second monomers forms part of a three-helix bundle protein domain. 31. FcRn binding dimer according to item 30, wherein said BM essentially forms part of two helices with an interconnecting loop, within said three-helix bundle protein domain. 32. FcRn binding dimer according to item 31, wherein said three—helix bundle protein domain is selected from bacterial receptor s. 33. FcRn binding dimer ing to item 32, wherein said three—helix bundle protein domain is selected from domains of protein A from Staphylococcus aureus or derivatives thereof. 34. FcRn binding dimer according to any preceding item, wherein at least one of said first and second monomer units comprises a g module (BMod), which module ts of an amino acid sequence selected from: iii) K-[BM]—DPSQS XaXbLLXC EAKKL XdXeXfQ; wherein [BM] is an FcRn binding motif as defined in any one of items 1—29, provided that X29 is D; X3 is selected from A and S; Xb is ed from N and E; X0 is selected from A, S and C; Xd is ed from E, N and S; Xe is selected from D, E and S; Xf is selected from A and S; iv) an amino acid ce which has at least 93 % identity to a sequence defined by iii).
. FcRn binding dimer ing to any one of items 1-33, wherein at least one of said first and second monomer units comprises a g module (BMod), which module ts of an amino acid sequence selected from: v) K-[BM]—QPEQS XaXbLLXC EAKKL XdXeXfQ; wherein [BM] is an FcRn binding motif as defined in any one of items 1-29, provided that X29 is R; X8 is selected from A and S; Xb is selected from N and E; XC is selected from A, S and C; Xd is selected from E, N and S; Xe is selected from D, E and S; Xf is selected from A and S; vi) an amino acid sequence which has at least 93 % identity to a sequence defined by v). 36. FcRn g dimer according to item 34, wherein at least one of said first and second monomer units comprises a BMod corresponding to the sequence from position 7 to position 55 in a sequence selected from the group consisting of SEQ ID NO:1-353, SEQ ID NO:358 and SEQ ID N01360- 364, such as the group consisting of SEQ ID NO:17-352 and SEQ ID NO:360-364. 37. FcRn binding dimer according to item 36, wherein at least one of said first and second monomer units comprises a BMod corresponding to the sequence from position 7 to position 55 in a sequence selected from the group consisting of SEQ ID NO:1-15, SEQ ID NO:17-140, SEQ ID NO:353, SEQ ID NO:358 and SEQ ID -364. 38. FcRn binding dimer according to item 37, wherein at least one of said first and second monomer units ses a BMod ponding to the sequence from position 7 to position 55 in a sequence selected from the group consisting of SEQ ID NO:1-2, SEQ ID NO:17-140, SEQ ID NO:358 and SEQ ID NO:360—364, such as the group consisting of SEQ ID 140 and SEQ ID NO:360-364. 39. FcRn binding dimer according to item 38, wherein at least one of said first and second monomer units comprises a BMod corresponding to the sequence from position 7 to position 55 in a sequence selected from the group consisting of SEQ ID NO:1-2, SEQ ID NO:17-92, SEQ ID NO:94-103, SEQ ID NO:105-125, SEQ ID NO:127-140, SEQ ID NO:358 and SEQ ID NO:360-364, such as the group consisting of SEQ ID NO:17-92, SEQ ID NO:94-103, SEQ ID NO:105-125, SEQ ID NO:127-140 and SEQ ID - 364. 40. FcRn binding dimer according to item 36, wherein at least one of said first and second monomer units comprises a BMod corresponding to the sequence from position 7 to on 55 in a sequence selected from the group consisting of SEQ ID NO:1-8, SEQ ID NO:13, SEQ ID NO:19-20, SEQ ID NO:23, SEQ ID NO:28, SEQ ID NO:41, SEQ ID NO:44, SEQ ID NO:65, SEQ ID NO:70, SEQ ID NO:73, SEQ ID NO:75-77, SEQ ID NO:353, SEQ ID NO:358 and SEQ ID NO:360-364, such as the group consisting of SEQ ID NO:19—20, SEQ ID NO:23, SEQ ID NO:28, SEQ ID NO:41, SEQ ID NO:44, SEQ ID NO:65, SEQ ID NO:70, SEQ ID NO:73, SEQ ID NO:75-77 and SEQ ID NO:360-364. 41. FcRn binding dimer according to item 39 or 40, wherein at least one of said first and second monomer units comprises a BMod corresponding to the sequence from position 7 to on 55 in a sequence selected from the group consisting of SEQ ID NO:1, SEQ ID NO:20, SEQ ID NO:23, SEQ ID NO:28, SEQ ID NO:41, SEQ ID NO:44, SEQ ID NO:65, SEQ ID NO:73, SEQ ID NO:75-77, SEQ ID NO:358 and SEQ ID —364, such as the group consisting of SEQ ID NO:20, SEQ ID NO:23, SEQ ID NO:28, SEQ ID NO:41, SEQ ID NO:44, SEQ ID NO:65, SEQ ID NO:73, SEQ ID NO:75-77 and SEQ ID NO:360-364. 42. FcRn binding dimer according to item 41, wherein at least one of said first and second monomer units comprises a BMod corresponding to the sequence from position 7 to position 55 in a sequence ed from the group consisting of SEQ ID NO:1, SEQ ID NO:20, SEQ ID NO:23, SEQ ID NO:41, SEQ ID NO:44, SEQ ID NO:65, SEQ ID NO:75,SEQ ID NO:77, SEQ ID NO:358 and SEQ ID NO:360-364, such as the group ting of SEQ ID NO:20, SEQ ID NO:23, SEQ ID NO:41, SEQ ID NO:44, SEQ ID NO:65, SEQ ID NO:75, SEQ ID NO:77 and SEQ ID -364. 43. FcRn binding dimer according to item 42, wherein at least one of said first and second monomer units comprises a BMod corresponding to the sequence from position 7 to position 55 in a sequence selected from the group ting of SEQ ID NO:1, SEQ ID NO:20, SEQ ID NO:23, SEQ ID NO:41, SEQ ID NO:44, SEQ ID NO:65, SEQ ID NO:75, SEQ ID NO:358 and SEQ ID NO:360-364, such as the group consisting of SEQ ID NO:20, SEQ ID NO:23, SEQ ID NO:41, SEQ ID NO:44, SEQ ID NO:75 and SEQ ID NO:360- 364. 44. FcRn binding dimer according to item 43, wherein at least one of said first and second monomer units comprises a BMod corresponding to the sequence from position 7 to position 55 in a sequence selected from the group consisting of SEQ ID NO:1, SEQ ID NO:23, SEQ ID NO:75, SEQ ID NO:358, SEQ ID NO:361 and SEQ ID NO:364, such as the group consisting of SEQ ID NO:23, SEQ ID NO:75, SEQ ID NO:361 and SEQ ID NO:364. 45. FcRn binding dimer according to item 43, wherein at least one of said first and second monomer units comprises a BMod corresponding to the sequence from position 7 to position 55 in a sequence selected from the group consisting of SEQ ID NO:20, SEQ ID NO:41, SEQ ID NO:44, SEQ ID , SEQ ID NO:362 and SEQ ID NO:363, such as the group consisting of SEQ ID NO:20, SEQ ID NO:41, SEQ ID NO:360 and SEQ ID ; the group consisting of SEQ ID NO:20, SEQ ID NO:44, SEQ ID NO:360 and SEQ ID NO:363; or the group consisting of SEQ ID NO:41, SEQ ID NO:44, SEQ ID NO:362 and SEQ ID . 46. FcRn binding dimer according to item 43, wherein at least one of said first and second monomer units comprises a BMod ponding to the sequence from position 7 to position 55 in a sequence selected from the group consisting of SEQ ID NO:1, SEQ ID NO:23 ,SEQ ID NO:44, SEQ ID NO:358, SEQ ID NO:361 and SEQ ID NO:363, such as the group consisting of SEQ ID NO:23,SEQ ID NO:44, SEQ ID NO:361 and SEQ ID NO:363. 47. FcRn binding dimer according to item 46, wherein at least one of said first and second monomer units ses a BMod corresponding to the sequence from on 7 to position 55 in SEQ ID NO:44. 48. FcRn binding dimer according to any one of items 1-13 and 15-47, wherein both said first and second r units comprise a BMod corresponding to the sequence from position 7 to position 55 in a sequence selected from the group as defined in any one of items 36-47. 49. FcRn binding dimer according to item 48, wherein both said first and second monomer units comprise a BMod corresponding to the sequence from position 7 to position 55 in a sequence selected from the group W0 2016;042083 consisting of SEQ ID NO:20, SEQ ID NO:23, SEQ ID NO:41, SEQ ID NO:44 , SEQ ID NO:75 and SEQ ID NO:360—364, such as the group consisting of SEQ ID NO:20, SEQ ID NO:41, SEQ ID NO:44, SEQ ID NO:360, SEQ ID NO:362 and SEQ ID NO:363. 50. FcRn binding dimer according to item 49, wherein both said first and second monomer units se a BMod corresponding to the sequence from on 7 to position 55 in SEQ ID NO:44. 51. FcRn binding dimer according to any preceding item, n at least one of said first and second r units comprises a sequence selected from the group consisting of: vii) YAK-[BM-DPSQS SELLXC EAKKL NDSQA P; wherein [BM] is an FcRn binding motif as defined in any one of items 1-29 and XC is selected from A, S and C; and viii) an amino acid sequence which has at least 94 % identity to a sequence d by vii). 52. FcRn binding dimer according to any one of items 1—50, wherein at least one of said first and second monomer units comprises a sequence selected from the group consisting of: ix) FAK-[BM]-DPSQS SELLXC EAKKL SESQA P; n [BM] is an FcRn binding motif as defined in any one of items 1-29 and XC is selected from A, S and C; and x) an amino acid sequence which has at least 94 % identity to a sequence defined by ix). 53. FcRn binding dimer according to any one of items 1-50, wherein at least one of said first and second monomer units comprises a sequence selected from the group consisting of: Xi) FNK—[BM—DPSQS ANLLXC EAKKL NDAQA P; WO 42083 wherein [BM] is an FcRn binding motif as d in any one of items 1-29 and XC is ed from A and C; and xii) an amino acid sequence which has at least 94 % identity to a sequence defined by xi). 54. FcRn binding dimer according to item 33, wherein at least one of said first and second monomer units comprises a sequence selected from: ADNNFNK-[BM]—DPSQSANLLSEAKKLNESQAPK; ADNKFNK-[BM]—DPSQSANLLAEAKKLNDAQAPK; ADNKFNK-[BMJ-DPSVSKEILAEAKKLNDAQAPK; ADAQQNNFNK-[BM]-DPSQSTNVLGEAKKLNESQAPK; AQHDE-[BM]-DPSQSANVLGEAQKLNDSQAPK; VDNKFNK-[BMj-DPSQSANLLAEAKKLNDAQAPK; AEAKYAK-[BMj-DPSESSELLSEAKKLNKSQAPK; VDAKYAK-[BMj-DPSQSSELLAEAKKLNDAQAPK' VDAKYAK-[BMJ-DPSQSSELLAEAKKLNDSQAPK' AEAKYAK-[BMj-DPSQSSELLSEAKKLNDSQAPK; AEAKYAK-[BMj-DPSQSSELLSEAKKLNDSQAP; AEAKFAK-[BMj-DPSQSSELLSEAKKLNDSQAPK; AEAKFAK-[BMj-DPSQSSELLSEAKKLNDSQAP; AEAKYAK-[BMj-DPSQSSELLAEAKKLNDAQAPK; AEAKYAK-[BMj-DPSQSSELLSEAKKLSESQAPK; K-[BMj-DPSQSSELLSEAKKLSESQAP; AEAKFAK-[BM]—DPSQSSELLSEAKKLSESQAPK; AEAKFAK-[BM]—DPSQSSELLSEAKKLSESQAP; AEAKYAK-[BM]—DPSQSSELLAEAKKLSEAQAPK; AEAKYAK-[BMJ-QPEQSSELLSEAKKLSESQAPK; AEAKYAK-[BM]—DPSQSSELLSEAKKLESSQAPK; AEAKYAK-[BMJ-DPSQSSELLSEAKKLESSQAP; AEAKYAK-[BM]—DPSQSSELLAEAKKLESAQAPK; AEAKYAK-[BMJ—QPEQSSELLSEAKKLESSQAPK; AEAKYAK-[BM]—DPSQSSELLSEAKKLSDSQAPK; AEAKYAK-[BMj-DPSQSSELLSEAKKLSDSQAP; AEAKYAK-[BM]—DPSQSSELLAEAKKLSDAQAPK; AEAKYAK-[BMJ—QPEQSSELLSEAKKLSDSQAPK; VDAKYAK-[BM]—DPSQSSELLSEAKKLNDSQAPK; WO 42083 VDAKFAK-[BMj-DPSQSSELLSEAKKLNDSQAPK; VDAKYAK-[BM]—DPSQSSELLAEAKKLNDAQAPK; VDAKYAK-[BMj-DPSQSSELLSEAKKLSESQAPK; VDAKFAK-[BM]—DPSQSSELLSEAKKLSESQAPK; VDAKYAK-[BMj-DPSQSSELLAEAKKLSEAQAPK; VDAKYAK-[BM]—QPEQSSELLSEAKKLSESQAPK; VDAKYAK-[BMj-DPSQSSELLSEAKKLESSQAPK; K-[BM]—DPSQSSELLAEAKKLESAQAPK; VDAKYAK-[BMj—QPEQSSELLSEAKKLESSQAPK; VDAKYAK-[BM]—DPSQSSELLSEAKKLSDSQAPK; VDAKYAK-[BMj-DPSQSSELLAEAKKLSDAQAPK; VDAKYAK-[BMJ—QPEQSSELLSEAKKLSDSQAPK; VDAKYAK-[BMj-DPSQSSELLAEAKKLNKAQAPK; AEAKYAK-[BM]-DPSQSSELLAEAKKLNKAQAPK; and ADAKYAK-[BM]—DPSQSSELLSEAKKLNDSQAPK; wherein [BM] is an FcRn binding motif as defined in any one of items 1—29. 55. FcRn g dimer according to any preceding item, wherein at least one of said first and second monomer units comprises a sequence selected from the group consisting of: xiii) AEAKYAK-[BM-DPSQSSELLSEAKKLNDSQAPK; n [BM] is an FcRn binding motif as d in any one of items 1-29; xiv) an amino acid sequence which has at least 94 % identity to the sequence defined in xiii). 56. FcRn binding dimer according to item 55, wherein at least one of said first and second monomer units comprises a sequence xiii) selected from the group consisting of SEQ ID NO:354-357, such as selected from SEQ ID NO:354 and 357. 57. FcRn binding dimer according to item 56, wherein at least one of said first and second monomer units comprises a sequence xiii) which is SEQ ID NO:357.
W0 2016;042083 2015/071339 58. FcRn binding dimer according to any one of items 1-54, wherein at least one of said first and second monomer units comprises a sequence selected from the group consisting of: xv) AEAKFAK-[BM]—DPSQSSELLSEAKKLSESQAPK; wherein [BM] is an FcRn binding motif as defined in any one of items 1-29; xvi) an amino acid sequence which has at least 94 % identity to the sequence defined in xv). 59. FcRn binding dimer according to item 58, wherein at least one of said first and second monomer units comprises a sequence xv) selected from the group consisting of SEQ ID NO:365-367. 60. FcRn binding dimer according to any one of items 1-54, wherein at least one of said first and second monomer units comprises a sequence ed from the group consisting of: xvii) VDAKYAK-[BM]—DPSQSSELLSEAKKLSESQAPK; wherein [BM] is an FcRn binding motif as defined in any one of items 1—29; xviii) an amino acid ce which has at least 94 % identity to the sequence defined in xvii). 61. FcRn binding dimer according to item 60, wherein at least one of said first and second monomer units comprises a sequence xvii) selected from the group ting of SEQ ID NO:360-364. 62. FcRn g dimer according to any preceding item, n at least one of said first and second monomer units comprises a sequence selected from the group consisting of: xix) AEAKYAK-[BM-RQPESSELLSEAKKLSESQAPK; n [BM] is an FcRn binding motif as defined in any one of items 1-29; xx) an amino acid sequence which has at least 94 % identity to the sequence defined in xix). 63. FcRn binding dimer according to item 62, wherein at least one of said first and second monomer units comprises a sequence xix) which is SEQ ID NO:359. 64. FcRn binding dimer according to any one of items 1-54, wherein at least one of said first and second monomer units comprises a sequence selected from the group consisting of: XXi) VDAKYAK-[BM-DPSQSSELLSEAKKLNDSQAPK; wherein [BM] is an FcRn binding motif as d in any one of items 1-29; xxii) an amino acid sequence which has at least 94 % identity to the ce d in xxi). 65. FcRn binding dimer according to item 64, wherein at least one of said first and second monomer units comprises a sequence xxi) selected from the group consisting of SEQ ID NO:1-353, such as the group consisting of SEQ ID NO:17-352. 66. FcRn binding dimer according to item 65, wherein at least one of said first and second monomer units comprises a sequence xxi) selected from the group ting of SEQ ID NO:1-15, SEQ ID NO:17-140 and SEQ ID NO:353, or comprises a sequence xxi) selected from the group consisting of SEQ ID NO:1-2 and SEQ ID NO:17-140, such as the group consisting of SEQ ID NO:17-140. 67. FcRn binding dimer according to item 66, wherein at least one of said first and second monomer units comprises a sequence xxi) selected from the group consisting of SEQ ID NO:1-2, SEQ ID NO:17-92, SEQ ID NO:94- 103, SEQ ID NO:105-125 and SEQ ID -140, such as the group consisting of SEQ ID NO:17-92, SEQ ID NO:94-103, SEQ ID NO:105-125 and SEQ ID NO:127-140. 2015/071339 68. FcRn binding dimer according to item 67, wherein at least one of said first and second monomer units comprises a ce xxi) selected from the group consisting of SEQ ID NO:1—8, SEQ ID NO:13, SEQ ID NO:19-20, SEQ ID NO:23, SEQ ID NO:28, SEQ ID NO:41, SEQ ID NO:44, SEQ ID NO:65, SEQ ID NO:70, SEQ ID NO:73, SEQ ID 77 and SEQ ID NO:353, such as the group consisting of SEQ ID NO:19-20, SEQ ID NO:23, SEQ ID NO:28, SEQ ID NO:41, SEQ ID NO:44, SEQ ID NO:65, SEQ ID NO:70, SEQ ID NO:73 and SEQ ID NO:75-77. 69. FcRn binding dimer according to item 66 or 68, wherein at least one of said first and second monomer units comprises a sequence xxi) selected from the group consisting of SEQ ID NO:1, SEQ ID NO:20, SEQ ID NO:23, SEQ ID NO:28, SEQ ID NO:41, SEQ ID NO:44, SEQ ID NO:65, SEQ ID NO:73 and SEQ ID NO:75-77, such as the group consisting of SEQ ID NO:20, SEQ ID NO:23, SEQ ID NO:28, SEQ ID NO:41, SEQ ID NO:44, SEQ ID NO:65, SEQ ID NO:73 and SEQ ID NO:75-77. 70. FcRn binding dimer according to item 69, wherein at least one of said first and second monomer units comprises a ce xxi) selected from the group consisting of SEQ ID NO:1, SEQ ID NO:20, SEQ ID NO:23, SEQ ID NO:41, SEQ ID NO:44, SEQ ID NO:65, SEQ ID NO:75 and SEQ ID NO:77, such as the group consisting of SEQ ID NO:20, SEQ ID NO:23, SEQ ID NO:41, SEQ ID NO:44, SEQ ID NO:65, SEQ ID NO:75 and SEQ ID NO:77. 71. FcRn g dimer according to item 70 wherein at least one of said first and second r units comprises a sequence xxi) selected from the group consisting of SEQ ID NO:1, SEQ ID NO:20, SEQ ID NO:23, SEQ ID NO:41, SEQ ID NO:44, SEQ ID NO:65 and SEQ ID NO:75, such as the group consisting of SEQ ID NO:20, SEQ ID NO:23, SEQ ID NO:41, SEQ ID NO:44, SEQ ID NO:65 and SEQ ID NO:75, such as the group consisting of SEQ ID NO:20, SEQ ID NO:23, SEQ ID NO:41, SEQ ID NO:44 and SEQ ID NO:75. 72. FcRn binding dimer according to item 71, wherein at least one of said first and second monomer units comprises a sequence xxi) selected from the group consisting of SEQ ID NO:1, SEQ ID N023 and SEQ ID NO:75, such as the group consisting ofSEQ ID N023 and SEQ ID NO:75. 73. FcRn binding dimer according to item 71, wherein at least one of said first and second monomer units comprises a sequence xxi) selected from the group consisting of SEQ ID NO:20, SEQ ID NO:41 and SEQ ID NO:44. 74. FcRn binding dimer ing to item 71, wherein at least one of said first and second monomer units comprises a sequence xxi) selected from the group consisting of SEQ ID NO:1, SEQ ID N023 and SEQ ID NO:44, such as the group consisting of SEQ ID N023 and SEQ ID NO:44. 75. FcRn binding dimer according to item 73 or 74, wherein at least one of said first and second monomer units comprises a sequence xxi) which is SEQ ID NO:44. 76. FcRn binding dimer according to any one of items 1-13 and 15-75, wherein both said first and second r units correspond to a ce ed from the group as defined in any one of items 55, 56 and 62-75. 77. FcRn binding dimer according to item 76, wherein both said first and second monomer units pond to a sequence selected from the group consisting of SEQ ID NO:1, SEQ ID NO:20, SEQ ID NO:23, SEQ ID NO:41, SEQ ID NO:44, SEQ ID NO:65, SEQ ID NO:75, SEQ ID NO:354, SEQ ID NO:357 and SEQ ID NO:360—367, such as the group consisting of SEQ ID NO:20, SEQ ID NO:41, SEQ ID NO:44, SEQ ID NO:360, SEQ ID , SEQ ID NO:363, SEQ ID NO:365, SEQ ID NO:366 and SEQ ID NO:367. 78. FcRn binding dimer according to item 77, wherein both said first and second monomer units pond to SEQ ID N021 or SEQ ID NO:357. 79. FcRn binding dimer according to item 77, wherein both said first and second monomer units correspond to SEQ ID NO:20, SEQ ID NO:360 or SEQ ID NO:365. 80. FcRn binding dimer according to item 77, wherein both said first and second monomer units correspond to SEQ ID NO:41, SEQ ID NO:362 or SEQ ID NO:366. 81. FcRn binding dimer ing to item 77, wherein both said first and second monomer units correspond to SEQ ID NO:44, SEQ ID NO:363 or SEQ ID NO:367. 82. FcRn binding dimer according to any preceding item, wherein said linker is selected from the group consisting of flexible amino acid linkers, rigid amino acid linkers and cleavable amino acid linkers. 83. FcRn binding dimer according to item 82, wherein said linker is arranged between said first monomer unit and said second r unit. 84. FcRn binding dimer according to item 82 or 83, wherein said linker is a flexible linker comprising amino acid residues selected from the group consisting of glycine, serine and threonine.
W0 2016;042083 85. FcRn binding dimer according to claim 84, wherein said linker has a general formula selected from (GnSm)p and (SnGm)p, wherein, ndently, n=1-7, m=0-7, n+ms8and p=1-7. 86. FcRn binding dimer according to claim 85, wherein n = 1-5. 87. FcRn binding dimer according to any one of claims 85-86, wherein m = 0-5. 88. FcRn binding dimer ing to any one of claims 85-87, wherein p = 1-5. 89. FcRn binding dimer ing to any one of claims 86-88, wherein n=4,m=1andp=1-4. 90. FcRn binding dimer according to claim 89, wherein said linker is 91. FcRn binding dimer according item 89, wherein said linker is G48 92. FcRn binding dimer according to any preceding item, which is capable of binding to FcRn with at least 2 times, such as at least 3 times, such as at least 4 times, such as at least 5 times, such as at least 6 times, such as at least 7 times, such as at least 8 times, such as at least 9 times, such as at least 10 times, such as at least 25 times, such as at least 50 times, such as at least 100 times higher capacity than the corresponding first monomer unit or second monomer unit alone. 93. FcRn binding dimer according to item 92, which is capable of binding to FcRn at pH 6.0 with at least 2 times, such as at least 3 times higher capacity than the corresponding first monomer unit or second monomer unit alone. 94. FcRn binding dimer according to item 92, which is capable of binding to FcRn at pH 7.4 with at least 2 times, such as at least 3 times, such as at least 4 times, such as at least 5 times, such as at least 6 times, such as at least 7 times, such as at least 8 times, such as at least 9 times, such as at least 10 times higher capacity than the corresponding first r unit or second monomer unit alone.
W0 2016;042083 95. FcRn binding dimer according to any preceding item, which is capable of binding to FcRn at pH 6.0 such that the KB value of the interaction is at most 1 X 10'7 M, such as at most 1 X 10'8 M, such as at most 1 X 10'9 M, such as at most 1 X 10‘10 M, such as at most 1 X 10‘11 M, such as at most 1 x 10'12 M. 96. FcRn g dimer according to any preceding item, wherein the KB value of the interaction between FcRn binding polypeptide and FcRn at pH 7.4 is higher than the K0 value of said interaction at pH 6.0, such as at least 2 times higher, such as at least 5 times higher, such as at least 10 times higher, such as at least 50 times higher, such as at least 100 times higher than the KB value of said interaction at pH 6.0. 97. FcRn g dimer according to any preceding item, wherein the KB value of the interaction between FcRn binding polypeptide and FcRn at pH 7.4 is at least 1 X 10'10 M, such as at least 1 X 10'9 M, such as at least 1 X 10'8 M, such as at least 1 X 10'7 M, such as at least 1 X 10'6 M, such as at least 1 x 10'5 M. 98. FcRn binding dimer ing to any one of items 1-94, wherein the KD value of said interaction at pH 7.4 is the same as or lower than the KD value of said interaction at pH 6.0. 99. FcRn g dimer according to any one of items 1—94, n the KB value of said interaction at pH 7.4 is at most 1 X 10'7 M, such as at most 1 X 10'8 M, such as at most 1 X 10'9 M, such as at most 1 X 10'10 M, such as at most 1 X 10'11 M, such as at most 1 X 10'12 M. 100. FcRn binding dimer according to any preceding item, wherein at least one of said first and second monomer units comprises at least one additional amino acid at the C-terminal and/or N-terminal end. 101. FcRn binding dimer according to item 100, wherein said at least one additional amino acid extension improves or simplifies production, purification, ization in vivo or in vitro, coupling or detection of the polypeptide. 102. Fusion protein or conjugate sing - a first moiety consisting of an FcRn binding dimer according to any preceding item; and - a second moiety consisting of a polypeptide having a desired biological activity.
W0 42083 103. Fusion protein or conjugate according to item 102, wherein the in vivo half—life of said fusion protein or conjugate is longer than the in vivo half- life of the polypeptide having a desired biological activity per se. 104. Fusion protein or conjugate according to any one of items 102- 103, wherein said d biological ty is a therapeutic activity. 105. Fusion protein or conjugate according to any one of items 100- 102, n said desired biological activity is a binding activity to a selected target. 106. Fusion protein or conjugate according to item 105, wherein said selected target is albumin. 107. Fusion protein or ate according to item 106, wherein said albumin binding activity is provided by the albumin binding domain of streptococcal protein G, or a derivative thereof. 108. Fusion protein or conjugate according to any one of items 106- 107, wherein said albumin binding activity increases in vivo half-life of the fusion protein or conjugate. 109. Fusion protein or conjugate according to any one of items 103- 104, wherein said d biological activity is an tic activity. 110. Fusion protein or conjugate according to any one of items 103- 105, wherein the second moiety having a desired biological activity is a therapeutically active polypeptide. 111. Fusion protein or conjugate according to any one of items 103- 105 and 109-110, wherein the second moiety having a desired biological activity is selected from the group consisting of enzymes, hormones, growth factors, chemokines and cytokines. 112. FcRn g dimer, fusion protein or conjugate according to any preceding item, which inhibits g of lgG to FcRn. 113. FcRn binding dimer, fusion protein or conjugate ing to item 112, which binds FcRn such that the ability of the FcRn binding dimer to block lgG binding to FcRn is at least 2 times higher, such as at least 3 times higher, such as at least 4 times higher, such as at least 5 times higher, such as at least 10 times, such as at least 15 times, such as at least 20 times, such as at least 25 times higher ed to the blocking y of the corresponding first or second monomer unit alone. 114. FcRn binding dimer, fusion protein or conjugate according to item 112 or 113, wherein the KB value of the interaction between said FcRn W0 2016;042083 2015/071339 binding ptide, fusion protein or conjugate and FcRn is lower than the KB value of the ction between lgG and FcRn. 115. FcRn g dimer, fusion protein or conjugate according to any preceding item, further comprising a label. 116. FcRn binding dimer, fusion protein or conjugate according to item 115, n said label is selected from the group consisting of scent dyes and metals, chromophoric dyes, chemiluminescent compounds and bioluminescent proteins, enzymes, radionuclides and radioactive particles. 117. FcRn binding dimer, fusion protein or conjugate according to any preceding item, comprising a chelating environment provided by a polyaminopolycarboxylate chelator conjugated to the FcRn binding polypeptide via a thiol group of a cysteine residue or an amine group of a lysine residue. 118. FcRn binding dimer, fusion protein or conjugate according to item 117, wherein the polyaminopolycarboxylate chelator is 1,4,7,10- tetraazacyclododecane-1,4,7,10-tetraacetic acid or a derivative thereof. 119. FcRn binding dimer, fusion protein or conjugate according to item 118, wherein the 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid derivative is 10-tetraazacyclododecane-1,4,7-tris-acetic 0- maleimidoethylacetamide. 120. FcRn binding dimer, fusion protein or conjugate according to item 117, wherein the polyaminopolycarboxylate chelator is 1,4,7- triazacyclononane-1,4,7-triacetic acid or a derivative f. 121. FcRn binding dimer, fusion protein or conjugate according to item 117, n the polyaminopolycarboxylate chelator is diethylenetriaminepentaacetic acid or derivatives thereof. 122. A cleotide encoding a polypeptide according to any one of items 1-114. 123. Expression vector comprising a polynucleotide according to item 122. 124. Host cell comprising an expression vector according to item 123. 125. Method of producing a polypeptide according to any one of items 1-114, comprising - culturing a host cell according to item 124 under conditions permissive of expression of said polypeptide from said expression vector, and - isolating said polypeptide.
W0 2016;042083 126. Composition comprising an FcRn binding dimer, fusion protein or conjugate according to any one of items 1-121 and at least one pharmaceutically acceptable excipient or r. 127. Composition according to item 126, further comprising at least one additional active agent. 128. Composition according to any one of items 126-127, which is adapted for administration by a route selected from the group consisting of oral administration, asal administration, pulmonar administration, vaginal stration, rectal administration, intravenous injection, intraperitoneal injection, uscular injection, subcutaneous ion and intradermal ion. 129. FcRn binding dimer, fusion protein or conjugate according to any one of items 1-121 or composition according to any one of items 126-128 for use as a medicament. 130. FcRn binding dimer, fusion protein, conjugate or composition for use ing to item 129, wherein said medicament is intended for treatment or laxis of an auto-immune condition. 131. FcRn binding dimer, fusion protein, conjugate or composition for use according to item 129, wherein said medicament is intended for treatment or prophylaxis of an allo-immune condition. 132. FcRn binding dimer, fusion protein, conjugate or composition for use according to item 129, wherein said medicament is intended for treatment or prophylaxis of a condition selected from the group consisting of epilepsy and seizures. 133. Method of treatment or prophylaxis of a subject in need thereof, comprising administering to the t a therapeutically or prophylactically active amount of an FcRn binding dimer, fusion protein or conjugate according to any one of items 1-121 or composition ing to any one of items 8. 134. Method according to item 133, for ent or prophylaxis of an auto-immune condition. 135. Method according to item 133, for treatment or prophylaxis of an allo-immune condition. 136. Method according to item 133, for treatment or prophylaxis of a condition selected from the group consisting of epilepsy and seizures.

Claims (19)

1. FcRn binding dimer, sing a first monomer unit, a second monomer unit and an amino acid linker, wherein said first and second 5 monomer unit each comprises an FcRn binding motif BM, which motif consists of the amino acid sequence EX2 X3 X4 AX6 X7 EIR WLPNLX16X17 X18 QR X21 AFIX25 X26LX28 X29 10 wherein, independently from each other, X2 is selected from A, D, E, F, H, I, K, L, N, Q, R, S, T, V, W and Y; X3 is selected from A, D, E, F, G, H, I, K, L, M, N, Q, R, S, T, V, W and 15 X4 is selected from A, D, E, F, G, H, I, K, L, N, Q, R, S, T, V, W and Y; X6 is selected from A, E, F, G, H, I, K, Q, R, S and V; X7 is selected from A, F, H, K, N, Q, R, S and V; X16 is selected from N and T; X17 is selected from F, W and Y; 20 X18 is ed from A, D, E and N; X21 is selected from A, S, V and W; X25 is selected from D, E, G, H, I, K, L, N, Q, R, S, T, V, W and Y; X26 is ed from K and S; X28 is selected from D, E, F, H, I, K, L, N, Q, R, S, T, V, W and Y; and 25 X29 is selected from D and R, and wherein said FcRn binding dimer binds FcRn with a higher binding capacity compared to said first r or said second monomer alone. 30
2. FcRn binding dimer according to claim 1, wherein the BM of at least one of said first and second monomer units consists of an amino acid sequence selected from i) EX2 X3 X4 AX6 HEIR WLPNLTX17 X18 QR X21 AFIX25 KLX28 D wherein, independently from each other, X2 is selected from A, D, E, F, H, I, K, L, N, Q, R, S, T, V, W and Y; X3 is selected from A, D, E, G, H, K, L, M, N, Q, R, S, T, V and Y; X4 is ed from A, D, E, F, G, I, K, L, N, Q, R, S, T, V and Y; X6 is selected from A, G, K, R, S and V; 5 X17 is selected from F, W and Y; X18 is selected from A, D, E and N; X21 is selected from A, S, V and W; X25 is selected from D, G, H, K, L, N, R, V and W; X28 is selected from D, E, H, K, L, N, Q, R, S, T, W and Y; ii) an amino acid sequence which has at least 96 % identity to a sequence defined by i), ed that X28 is not A.
3. FcRn binding dimer according to any one of the preceding claims, wherein said first and second monomer units comprise identical BM ces. 20
4. FcRn g dimer according to any one of claims 1-2, wherein said first and second monomer units comprise different BM sequences.
5. FcRn g dimer according to any one of the preceding claims, wherein at least one of said first and second monomer units comprises an 25 FcRn binding motif BM ponding to the sequence from position 8 to position 36 in a sequence selected from the group consisting of SEQ ID NO:2-205, 207-306 and 308-353, such as the group consisting of SEQ ID NO:17-205, 207-306 and 308-352, such as the group consisting of SEQ ID NO:17-140, such as the group consisting of SEQ ID NO:17-92, SEQ ID 30 NO:94-103, SEQ ID NO:105-125 and SEQ ID NO:127-140, such as the group consisting of SEQ ID NO:19-20, SEQ ID NO:23, SEQ ID NO:28, SEQ ID NO:41, SEQ ID NO:44, SEQ ID NO:65, SEQ ID NO:70, SEQ ID NO:73, SEQ ID NO:75-77, such as the group consisting of SEQ ID NO:20, SEQ ID NO:23, SEQ ID NO:28, SEQ ID NO:41, SEQ ID NO:44, SEQ ID NO:65, SEQ ID 35 NO:73 and SEQ ID NO:75-77, such as the group consisting of SEQ ID NO:20, SEQ ID NO:23, SEQ ID NO:41, SEQ ID NO:44, SEQ ID NO:65, SEQ ID NO:75 and SEQ ID NO:77, such as the group consisting of SEQ ID NO:20, SEQ ID NO:23, SEQ ID NO:41, SEQ ID NO:44and SEQ ID NO:75, such as the group consisting of SEQ ID NO:20, SEQ ID NO:41 and SEQ ID NO:44.
6. FcRn binding dimer according to claim 5, wherein at least one of 5 said first and second monomer units comprises an FcRn g motif BM ponding to the sequence from position 8 to position 36 in a sequence selected from the group ting of SEQ ID NO:20, SEQ ID NO:23, SEQ ID NO:41, SEQ ID NO:44, SEQ ID NO:65, SEQ ID NO:75 and SEQ ID NO:77, such as the group consisting of SEQ ID NO:20, SEQ ID NO:23, SEQ ID 10 NO:41, SEQ ID NO:44, SEQ ID NO:65 and SEQ ID NO:75, such as the group consisting of SEQ ID NO:20, SEQ ID NO:41 and SEQ ID NO:44, such as SEQ ID NO:44.
7. FcRn binding dimer according to any one of the preceding claims, 15 wherein at least one of said first and second monomer units comprises a sequence selected from the group consisting of: xi) AEAKYAK-[BM]-DPSQSSELLSEAKKLNDSQAPK; xv) AEAKFAK-[BM]-DPSQSSELLSEAKKLSESQAPK; 20 xvii) VDAKYAK-[BM]-DPSQSSELLSEAKKLSESQAPK; wherein [BM] is an FcRn binding motif as defined in any one of claims 1-2; 25 an amino acid sequence which has at least 94% identity to the n of the sequence outside the [BM] defined in xi), xv) or xvii), provided that X28 in [BM] is not A.
8. FcRn binding dimer according to claim 7, wherein at least one of 30 said first and second monomer units comprises a sequence selected from the group consisting of SEQ ID NO:355-357 and SEQ ID -367, such as the group consisting of SEQ ID NO:357 and SEQ ID NO:360-367.
9. FcRn binding dimer according to claim 1 or 2, wherein at least one 35 of said first and second monomer units comprises a sequence selected from the group consisting of: xxi) VDAKYAK-[BM]-DPSQSSELLSEAKKLNDSQAPK; wherein [BM] is an FcRn binding motif as defined in any one of claims 1-2; xxii) an amino acid sequence which has at least 94% identity to the portion of the sequence outside the [BM] d in xxi), provided that X28 in [BM] is not A.
10 10. FcRn binding dimer according to claim 9, wherein at least one of said first and second monomer units comprises a sequence xxi) selected from the group ting of SEQ ID NO:2-205, 207-306 and 308-353, such as the group consisting of SEQ ID NO:17-205, 207-306 and 308-352, such as the group consisting of SEQ ID NO:17-140, such as the group consisting of SEQ 15 ID 92, SEQ ID NO:94-103, SEQ ID -125 and SEQ ID NO:127- 140, such as the group consisting of SEQ ID NO:19-20, SEQ ID NO:23, SEQ ID NO:28, SEQ ID NO:41, SEQ ID NO:44, SEQ ID NO:65, SEQ ID NO:70, SEQ ID NO:73, SEQ ID NO:75-77, such as the group consisting of SEQ ID NO:20, SEQ ID NO:23, SEQ ID NO:28, SEQ ID NO:41, SEQ ID NO:44, SEQ 20 ID NO:65, SEQ ID NO:73 and SEQ ID NO:75-77, such as the group consisting of SEQ ID NO:20, SEQ ID NO:23, SEQ ID NO:41, SEQ ID NO:44, SEQ ID NO:65, SEQ ID NO:75 and SEQ ID NO:77, such as the group consisting of SEQ ID NO:20, SEQ ID NO:41, SEQ ID NO:44 and SEQ ID NO:75, such as the group consisting of SEQ ID NO:20, SEQ ID NO:41 and 25 SEQ ID NO:44.
11. FcRn binding dimer according to claim 10, wherein at least one of said first and second monomer units comprises a sequence xxi) selected from the group consisting of SEQ ID NO:20, SEQ ID NO:23, SEQ ID NO:41, SEQ 30 ID NO:44, SEQ ID NO:65, SEQ ID NO:75 and SEQ ID NO:77, such as the group consisting of SEQ ID NO:20, SEQ ID NO:23, SEQ ID NO:41, SEQ ID NO:44, SEQ ID NO:65 and SEQ ID NO:75, such as the group consisting of SEQ ID NO:20, SEQ ID NO:41 and SEQ ID NO:44, such as SEQ ID NO:44. 35
12. FcRn g dimer according to claim 8, wherein said first and second monomer units comprise SEQ ID NO:365 and SEQ ID NO:365; SEQ ID NO:366 and SEQ ID ; or SEQ ID NO:367 and SEQ ID NO:367, respectively.
13. FcRn binding dimer according to any one of the preceding claims, 5 wherein said linker has a general formula selected from (GnSm)p and p, wherein, independently, n = 1-7, m = 0-7, 10 n + m ≤ 8 and p = 1-7.
14. FcRn binding dimer according to any one of the preceding claims, which is capable of binding to FcRn with at least 2 times, such as at least 3 15 times, such as at least 4 times, such as at least 5 times, such as at least 6 times, such as at least 7 times, such as at least 8 times, such as at least 9 times, such as at least 10 times, such as at least 25 times, such as at least 50 times, such as at least 100 times higher capacity than the corresponding first monomer unit or second monomer unit alone.
15. Fusion protein or conjugate comprising - a first moiety consisting of an FcRn g dimer according to any one of the preceding claims; and - a second moiety consisting of a polypeptide having a desired 25 biological activity.
16. FcRn binding dimer, fusion n or conjugate according to any one of the preceding claims, which inhibits binding of IgG to FcRn. 30
17. FcRn binding dimer, fusion protein or conjugate according to claim 16, which binds FcRn such that the ability of the FcRn binding dimer to block IgG binding to FcRn is at least 2 times higher, such as at least 3 times higher, such as at least 4 times higher, such as at least 5 times higher, such as at least 10 times, such as at least 15 times, such as at least 20 times, such as at 35 least 25 times higher compared to the ng ability of the corresponding first or second monomer unit alone.
18. Composition comprising an FcRn binding dimer, fusion protein or conjugate according to any one of the preceding claims and at least one pharmaceutically acceptable ent or carrier. 5
19. Use of a FcRn binding dimer, fusion protein or conjugate according to any one of claims 1-17 in the manufacture of a medicament for the treatment or laxis of a condition selected from the group consisting of auto-immune conditions, allo-immune conditions, epilepsy and seizures. 1 /38 2015/071339 9%: dEmmOmmflfl<_-MT| m<>mOQMU .L/m. EE<¢ <DOEM<WV flflmzznmv JZmJBMHmflw<DMQHM<WM<fl soapmcmamwn mamwom (V) OWOOOMI—IOW I_I KO [\OWC\ L0 1— OW OKOV‘MOOI—IQ <I" L0 NOLO W KO I \l®\lm\l\l\ \ I \ mow \ I \ Ol\Ol\ OO O O [\ O O O IO\IO\. |\ \ I x \IO \ \ I x NNN L\_ N N N L\_ N WO 42083
NZ729218A 2014-09-17 2015-09-17 Neonatal Fc Receptor Binding Dimer and Methods of Use NZ729218B2 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP14185140 2014-09-17
EP14185140.2 2014-09-17
PCT/EP2015/071339 WO2016042083A1 (en) 2014-09-17 2015-09-17 New polypeptides

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NZ729218A NZ729218A (en) 2021-02-26
NZ729218B2 true NZ729218B2 (en) 2021-05-27

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