NZ721147B2

NZ721147B2 - Bispecific antigen binding molecules

Info

Publication number: NZ721147B2
Application number: NZ721147A
Authority: NZ
Inventors: Johannes Auer; Peter Bruenker; Tanja Fauti; Christiane Jaeger; Christian Klein; Wolfgang Schaefer; Claudio Sustmann; Pablo Umana
Original assignee: Roche Glycart Ag
Priority date: 2011-08-23
Filing date: 2012-08-21
Publication date: 2020-05-29

Abstract

Discloses bispecific antigen binding molecules comprising two Fab fragments specific to a first antigen and a second Fab fragment specific to a second antigen fused to a first Fc domain subunit. One of the Fab fragments is a crossover fab fragment with a VH-CL/VLCH1 arrangment. The bispecific antigen binding molecule does not comprise a single chain Fab fragment. n binding molecule does not comprise a single chain Fab fragment.

Description

Bispecific antigen binding molecules Field of the Invention The present invention generally relates to bispecific antigen binding molecules. In addition, the present invention relates to polynucleotides encoding such bispecific antigen binding molecules, and s and host cells comprising such cleotides. The invention further relates to methods for producing the bispecific antigen binding molecules of the invention, and to methods of using these bispecific antigen g molecules in the treatment of disease. ound Bi- or multispecific antibodies capable of binding two or more antigens are known in the art.

Such multispecific binding ns can be generated by hybridoma cell fusion, al conjugation or recombinant DNA techniques.

Bispecific dies are of great interest for therapeutic ations, as they allow the simultaneous binding and inactivation of two or more target antigens, thereby obviating the need for combination ies. Another promising application of bispecific antibodies is as engagers of immune effector cells e.g. for cellular cancer immunotherapy. For this purpose, bispecific antibodies are designed which bind to a surface n on target cells and, for example, to an activating component of the T cell receptor (TCR) complex. The simultaneous binding of such an antibody to both of its targets will force a temporary interaction between target cell and T cell, causing activation of any cytotoxic T cyte (CTL) and subsequent lysis of the target cell.

Hence, the immune response is re-directed to the target cells, independently of e antigen presentation by the target cell or the specificity of the T cell as required for normal MHC- restricted activation of CTLs. In this context it is important that CTLs are only activated when a target cell is presenting the bispecific antibody to them, i.e. when the immunological synapse is mimicked, and not simply upon binding of the antibody to the T cell antigen.

A y of recombinant multispecific antibody formats have been developed in the recent past, including, for e, tetravalent IgG-single-chain variable fragment (scFv) s (see e.g.

Coloma & Morrison, Nat Biotechnol 15, 159-163 (1997)), tetravalent IgG-like dual-variable domain antibodies (Wu et al., Nat Biotechnol 25, 1290-1297 (2007)), or bivalent rat/mouse hybrid bispecific IgGs (see e.g. Lindhofer et al., J Immunol 155, 219-225 (1995)).

Also several bispecific formats wherein the antibody core structure (IgA, IgD, IgE, IgG or IgM) is no longer retained have been made. Examples include diabodies (see e.g. Holliger et al., Proc Natl Acad Sci USA 90, 6444-6448 (1995)), tandem scFv molecules (see e.g. Bargou et al., Science 321, 974-977 (2008)), and various derivatives thereof.

The multitude of formats that are being developed shows the great potential attributed to bispecific antibodies. The task of generating bispecific antibodies suitable for a particular purpose is, r, by no means trivial and subject to a number of considerations. For e, the valency and geometry of the antibody needs to be appropriately chosen, depending on the characteristics of the target antigens and the intended effect. As for all eutic antibodies, efficacy and toxicity have to be balanced, which es i.a. minimization of immunogenicity and optimization of pharmacokinetic properties of the antibody. Also, the desirablility of Fcmediated effects has to be considered. Furthermore, the production of bispecific antibody constructs at a clinically sufficient quantity and purity poses a major challenge, as the homodimerization of antibody heavy chains and/or the mispairing of antibody heavy and light chains of ent specificities upon co-expression decreases the yield of the correctly assembled construct and results in a number of non-functional side products from which the desired bispecific antibody may be difficult to separate.

Given the increasing number of possible applications of bispecific antibodies, and the difficulties and disadvantages associated with currently available ific antibodies, there remains a need for novel, improved or alternative formats of such les; and/or ific antibodies which at least provide the public with a useful choice.

In this specification where reference has been made to patent specifications, other external documents, or other sources of ation, this is generally for the purpose of providing a context for discussing the features of the invention. Unless specifically stated ise, reference to such external documents is not to be ued as an admission that such documents, or such sources of information, in any iction, are prior art, or form part of the common l knowledge in the art.

In the description in this specification reference may be made to subject matter that is not within the scope of the claims of the current application. That subject matter should be readily identifiable by a person skilled in the art and may assist in putting into practice the ion as defined in the claims of this application.

Summary of the Invention In a first aspect, the invention provides a bispecific antigen binding molecule, sing a first Fab nt which specifically binds to a first antigen, a second Fab fragment which specifically binds to a second antigen, and an Fc domain composed of a first and a second subunit capable of stable association; wherein a) the bispecific n binding molecule provides monovalent binding to the first and/or the second antigen, b) (i) the first Fab fragment is fused at its C-terminus to the N-terminus of the second Fab fragment, which is in turn fused at its C-terminus to the inus of the first Fc domain subunit, (ii) the second Fab fragment is fused at its C-terminus to the N-terminus of the first Fab fragment, which is in turn fused at its C-terminus to the N-terminus of the first Fc domain subunit, or (iii) the second Fab fragment is fused at its C-terminus to the N- terminus of the first Fc domain subunit, which is in turn fused at its C-terminus to the N- terminus of the first Fab fragment , c) in the first and/or the second Fab fragment one of the following replacements is made: (i) the variable domains VL and VH are replaced by each other, or (ii) the nt domains CL and CH1 are replaced by each other, provided that not the same replacement is made in the first and the second Fab fragment, and d) the bispecific antigen binding molecule does not comprise a single chain Fab fragment In a second aspect, the invention provides an isolated polynucleotide ng the bispecific antigen binding molecule of the first aspect.

In a third aspect, the ion provides an expression vector comprising the isolated polynucleotide of the second aspect.In a fourth aspect, the ion provides a host cell comprising the isolated polynucleotide of the second aspect or the sion vector of the third aspect, wherein the host cell is not a human cell within a human.

In a fifth aspect, the invention provides a method for producing the bispecific antigen binding molecule of the first aspect, comprising the steps of a) culturing the host cell of the fourth aspect under conditions suitable for the expression of the bispecific n binding le and b) recovering the bispecific antigen g molecule.

In a sixth aspect, the invention provides a pharmaceutical composition comprising the bispecific antigen binding le of the first aspect, and a pharmaceutically acceptable carrier.

Brief Description Broadly described is a ific antigen binding molecule, comprising a first Fab fragment which specifically binds to a first antigen, a second Fab fragment which specifically binds to a second antigen, and an Fc domain composed of a first and a second subunit capable of stable association; wherein a) the bispecific antigen binding molecule es monovalent g to the first and/or the second antigen, b) the first Fab fragment, the second Fab fragment and the first Fc domain subunit are fused to each other, and c) in the first and/or the second Fab fragment one of the following replacements is made: (i) the variable domains VL and VH are replaced by each other, (ii) the constant domains CL and CH1 are replaced by each other, or (iii) both the variable and constant domains VL-CL and VH-CH1 are replaced by each other, provided that not the same replacement is made in the first and the second Fab fragment.

In ular embodiments, the first Fab nt is fused at its C-terminus to the N-terminus of the second Fab fragment, which is in turn fused at its inus to the N-terminus of the first Fc domain subunit. In a more specific embodiment, the first Fab fragment is fused at the C-terminus of its heavy chain to the N-terminus of the heavy chain of the second Fab fragment, which is in turn fused at the inus of its heavy chain to the inus of the first Fc domain subunit.

In other embodiments, the second Fab fragment is fused at its C-terminus to the N-terminus of the first Fab fragment, which is in turn fused at its C-terminus to the N-terminus of the first Fc domain subunit. In a more specific ment, the second Fab fragment is fused at the C- terminus of its heavy chain to the N-terminus of the heavy chain of the first Fab fragment, which is in turn fused at the C-terminus of its heavy chain to the N-terminus of the first Fc domain subunit. In still other embodiments, the second Fab fragment is fused at its C-terminus to the N- terminus of the first Fc domain t, which is in turn fused at its C-terminus to the N- us of the first Fab fragment. In a more specific embodiment, the second Fab fragment is fused at the C-terminus of its heavy chain to the N-terminus of the first Fc domain subunit, which is in turn fused at its C-terminus to the N-terminus of the heavy chain of the first Fab fragment.

In embodiments wherein either the first Fab fragment is fused at the C-terminus of its heavy chain to the N-terminus of the heavy chain of the second Fab fragment which is in turn fused at the C-terminus of its heavy chain to the N-terminus of the first Fc domain subunit, or the second Fab fragment is fused at the C-terminus of its heavy chain to the N-terminus of the heavy chain of the first Fab fragment which is in turn fused at the C-terminus of its heavy chain to the N- terminus of the first Fc domain subunit, additionally the Fab light chain of the first Fab fragment and the Fab light chain of the second Fab fragment may be fused to each other, optionally via a peptide linker.

In one embodiment, the ement is made in the first Fab fragment. In some embodiments, the replacement is a replacement of the variable domains VL and VH by each other. In other embodiments the ement is a replacement of the constant domains CL and CH1 by each other.

In one embodiment the bispecific antigen binding molecule essentially consists of the first Fab fragment, the second Fab fragment, the Fc domain, and optionally one or more peptide linkers.

In ular embodiments, the bispecific antigen binding molecule comprises a third Fab fragment which specifically binds to the first or the second antigen. In one embodiment, the third Fab fragment is fused to the second Fc domain subunit. In a more specific embodiment, the third Fab fragment is fused at its C-terminus to the N-terminus of the second Fc domain subunit. In en even more specific embodiment, the third Fab fragment is fused at the C-terminus of its heavy chain to the N-terminus of the second Fc domain subunit. In one ment, the third Fab fragment specifically binds to the second antigen. In some embodiments the the second Fab fragment, the third Fab fragment and the Fc domain are part of an immunoglobulin molecule. In a specific such embodiment, the globulin molecule is an IgG class immunoglobulin molecule, more specifically an IgG1 or IgG4 subclass globulin molecule. In one embodiment, the immunoglobulin molecule is a human immunoglobulin molecule. In one embodiment, the bispecific n binding molecule essentially consists of a first Fab nt which specifically binds to the first antigen, an immunoglobulin molecule which specifically binds to the second antigen, and optionally one or more peptide linkers.

In one embodiment, the same replacement is made in Fab fragments that specifically bind to the same antigen. In a further embodiment, a replacement is made only in the first Fab fragment. In one ment, the bispecific antigen binding molecule provides monovalent binding to the first n. In one embodiment, the bispecific antigen binding molecule does not se a -chain Fab fragment.

In certain embodiments, the Fc domain comprises a modification promoting the association of the first and second Fc domain subunit. In a specific such embodiment, an amino acid residue in the CH3 domain of the first subunit of the Fc domain is replaced with an amino acid residue having a larger side chain volume, thereby generating a erance within the CH3 domain of the first subunit which is positionable in a cavity within the CH3 domain of the second subunit, and an amino acid residue in the CH3 domain of the second t of the Fc domain is replaced with an amino acid residue having a smaller side chain volume, thereby generating a cavity within the CH3 domain of the second subunit within which the protuberance within the CH3 domain of the first t is positionable. In one embodiment, the Fc domain is an IgG Fc domain, ically an IgG1 or IgG4 Fc domain. In one embodiment the Fc domain is human. In certain embodiments, the Fc domain is engineered to have altered binding affinity to an Fc receptor and/or altered effector function, as compared to a non-engineered Fc domain.

Also described is an isolated polynucleotide encoding a bispecific n binding molecule described or a fragment thereof. Also described are polypeptides encoded by the polynucleotides described. Further bed is an expression vector comprising the isolated polynucleotide described, and a host cell comprising the isolated polynucleotide or the expression vector described. In some ments the host cell is a eukaryotic cell, particularly a mammalian cell.

Also described is a method of producing the bispecific n g molecule described, comprising the steps of a) culturing the host cell described under conditions suitable for the expression of the bispecific antigen binding molecule and b) recovering the bispecific antigen binding le. Also described is a bispecific antigen binding molecule produced by the method described.

Also described is a pharmaceutical composition comprising the bispecific antigen binding molecule described and a pharmaceutically acceptable carrier.

Also described are methods of using the bispecific antigen g le and pharmaceutical composition described. Described is a bispecific antigen binding molecule or a pharmaceutical composition described for use as a medicament. Desribed is a bispecific antigen binding molecule or a ceutical composition described for use in the treatment of a disease in an individual in need thereof. In a specific embodiment the disease is .

Also described is the use of a bispecific antigen binding molecule bed for the manufacture of a medicament for the treatment of a disease in an individual in need thereof; as well as a method of treating a disease in an dual, comprising administering to said individual a therapeutically effective amount of a composition comprising the bispecific n binding molecule described in a pharmaceutically acceptable form. In a specific ment the disease is cancer. In any of the above embodiments the individual preferably is a mammal, particularly a human.

Brief Description of the gs FIGURE 1. Illustration of exemplary formats of the bispecific antigen binding molecules described. (A) “2+1” , with Crossfab fragment of different specificity fused to inus of a Fab fragment comprised in an antibody (“2+1 IgG Crossfab (N-terminal)”). (B) “1+1” format, with Crossfab fragment of different specificity fused to N-terminus of a Fab nt comprised in an antibody lacking the second Fab fragment (“1+1 IgG Crossfab (N-terminal)”) .

(C) “2+1” format as in (A), wherein the order of the Crossfab fragment, and the Fab fragment to which the Crossfab fragment is fused, is inverted (“2+1” IgG Crossfab (N-terminal), inverted”).

(D) “2+1” format, with Crossfab fragment of different specificity fused to C-terminus of an Fc domain subunit comprised in an antibody (“2+1 IgG ab (C-terminal)”). Black dot: optional modification in the Fc domain promoting heterodimerization.

FIGURE 2. (A, B) SDS PAGE (4-12% Tris-Acetate (A) or 4-12% Bis/Tris (B), NuPage Invitrogen, Coomassie-stained) of “1+1 IgG Crossfab (N-terminal), Fc(hole) P329G LALA / Fc(knob) wt” (anti-MCSP/anti-huCD3) (see SEQ ID NOs 1, 2, 3 and 4), non reduced (A) and reduced (B). (C) Analytical size exclusion chromatography dex 200 10/300 GL GE Healthcare; 2 mM MOPS pH 7.3, 150 mM NaCl, 0.02% (w/v) NaCl; 50 μg sample ed) of “1+1 IgG Crossfab (N-terminal), Fc(hole) P329G LALA / Fc(knob) wt” (anti-MCSP/antihuCD3 FIGURE 3. (A, B) SDS PAGE (4-12% Bis/Tris, NuPage Invitrogen, Coomassie-stained) of “2+1 IgG Crossfab minal)” MCSP/anti-huCD3) (see SEQ ID NOs 1, 3, 4 and 5), non reduced (A) and reduced (B). (C) Analytical size exclusion chromatography (Superdex 200 /300 GL GE Healthcare; 2 mM MOPS pH 7.3, 150 mM NaCl, 0.02% (w/v) NaCl; 50 μg sample injected) of “2+1 IgG Crossfab (N-terminal)” (anti-MCSP/anti-huCD3).

FIGURE 4. (A, B) SDS PAGE (4-12% Bis/Tris, NuPage Invitrogen, Coomassie-stained) of “2+1 IgG Crossfab (N-terminal), inverted” (anti-CEA/anti-huCD3) (see SEQ ID NOs 3, 8, 9 and 10), non reduced (A) and reduced (B). (C) Analytical size exclusion chromatography (Superdex 200 /300 GL GE Healthcare; 2 mM MOPS pH 7.3, 150 mM NaCl, 0.02% (w/v) NaCl; 50 μg sample injected) of “2+1 IgG Crossfab (N-terminal), inverted” (anti-CEA/anti-huCD3).

FIGURE 5. (A, B) Capillary electrophoresis (CE)-SDS gel analysis of “2+1 IgG Crossfab (C- terminal)” (anti-c-Met/anti-Her3) (see SEQ ID NOs 11, 12, 13, 14), non reduced (A) and reduced (B).

FIGURE 6. Simultaneous binding of bispecific constructs to the D3 domain of human MCSP and human CD3γ(G4S)5CD3ε–AcTev–Fc(knob)–Avi/Fc(hole). (A) Biacore assay setup; (B) measurement of “2+1 IgG Crossfab (N-terminal)”.

FIGURE 7. Levels of different cytokines measured in the atant of whole blood after treatment with 1 nM of different CD3-MCSP bispecific ucts (“2+1 IgG Crossfab (N- terminal)”, “(scFv)2”) or corresponding control IgGs in the presence (A, B) or absence (C, D) of Colo-38 tumor cells for 24 hours.

FIGURE 8. e expression level of the late activation marker CD25 on cynomolgus CD8+ T cells from two ent animals (cyno Nestor, cyno Nobu) after 43 hours incubation with the indicated concentrations of the “2+1 IgG Crossfab (N-terminal)” bispecific construct (targeting cynomolgus CD3 and human MCSP), in the presence or absence of human MCSP-expressing MV-3 tumor target cells (E:T ratio = 3:1). As controls, the reference IgGs (anti-cynomolgus CD3 IgG, anti-human MCSP IgG) or the iologic stimulus PHA-M were used.

FIGURE 9. Killing (as measured by LDH release) of -435 tumor cells upon co-culture with human pan T cells (E:T ratio = 5:1) and activation for 20 hours by different concentrations of the “2+1 IgG Crossfab (N-terminal)” and “(scFv)2” ific molecules and corresponding IgGs.

FIGURE 10. Killing (as measured by LDH release) of MDA-MB-435 tumor cells upon ure with human pan T cells (E:T ratio = 5:1), and activation for 21 hours by different concentrations of the bispecific constructs and corresponding IgGs. The CD3-MCSP bispecific “2+1 IgG Crossfab (N-terminal)” and “1+1 IgG Crossfab (N-terminal)” constructs, the )2” molecule and corresponding IgGs were compared.

FIGURE 11. Killing (as measured by LDH release) of huMCSP-positive MV-3 melanoma cells upon co-culture with human PBMCs (E:T ratio = 10:1), treated with different CD3-MCSP bispecific constructs (“2+1 IgG Crossfab (N-terminal)” and “(scFv)2”) for ~26 hours.

FIGURE 12. Examplary configurations of ific antigen binding molecules described having a linked light chain. (A) Illustration of the “2+1 IgG Crossfab (N terminal), linked light chain” molecule. (B) Illustration of the “1+1 IgG ab (N-terminal), linked light chain” molecule.

(C) Illustration of the “2+1 IgG Crossfab (N-terminal), ed, linked light chain” le. (D) Illustration of the “1+1 IgG Crossfab (N-terminal), inverted, linked light chain” molecule.

FIGURE 13. CE-SDS analyses. Electropherogram shown as SDS PAGE of “2+1 IgG Crossfab (N-terminal), linked light chain” (lane 1: reduced, lane 2: duced).

FIGURE 14. Analytical size exclusion chromatography of “2+1 IgG Crossfab (N-terminal), linked light chain” (final product). 20 µg sample “2+1 IgG Crossfab (N-terminal), linked light chain” were injected.

FIGURE 15. g (as measured by LDH release) of MCSP-positive MV-3 tumor cells upon co-culture by human PBMCs (E:T ratio = 10:1), treated with different CD3-MCSP bispecific constructs for ~ 44 hours. Human PBMCs were isolated from fresh blood of healthy volunteers.

FIGURE 16. Killing (as measured by LDH release) of ositive Colo-38 tumor cells upon co-culture by human PBMCs (E:T ratio = 10:1), treated with different CD3-MCSP bispecific constructs for ~22 hours. Human PBMCs were isolated from fresh blood of healthy volunteers.

FIGURE 17. Killing (as measured by LDH release) of MCSP-positive Colo-38 tumor cells upon ture by human PBMCs (E:T ratio = 10:1), treated with different CD3-MCSP bispecific constructs for ~22 hours. Human PBMCs were isolated from fresh blood of healthy volunteers.

FIGURE 18. g (as measured by LDH release) of MCSP-positive WM266-4 cells upon co- culture by human PBMCs (E:T ratio = 10:1), treated with different CD3-MCSP bispecific constructs for ~22 hours. Human PBMCs were isolated from fresh blood of healthy volunteers.

FIGURE 19. Surface expression level of the early activation marker CD69 (A) and the late tion marker CD25 (B) on human CD8+ T cells after 22 hours incubation with 10 nM, 80 pM or 3 pM of different CD3-MCSP ific constructs in the presence or absence of human MCSP-expressing 8 tumor target cells (E:T ratio = 10:1).

FIGURE 20. CE-SDS analyses. (A) Electropherogram shown as SDS-PAGE of 1+1 IgG ab minal); VL/VH ge (LC007/V9): a) non-reduced, b) reduced. (B) Electropherogram shown as SDS-PAGE of 1+1 CrossMab; CL/CH1 exchange (LC007/V9): a) reduced, b) duced. (C) Electropherogram shown as SDS-PAGE of 2+1 IgG Crossfab (N- terminal), inverted; CL/CH1 exchange (LC007/V9): a) reduced, b) non-reduced. (D) Electropherogram shown as SDS-PAGE of 2+1 IgG ab (N-terminal); VL/VH exchange (M4-3 ML2/V9): a) reduced, b) non-reduced. (E) Electropherogram shown as SDS-PAGE of 2+1 IgG Crossfab (N-terminal); CL/CH1 exchange (M4-3 ML2/V9): a) reduced, b) non-reduced.

(F) Electropherogram shown as SDS-PAGE of 2+1 IgG ab (N-terminal), inverted; CL/CH1 exchange (CH1A1A/V9): a) reduced, b) non-reduced.

FIGURE 21. Surface expression level of the early activation marker CD69 (A) or the late activation marker CD25 (B) on human CD4+ or CD8+ T cells after 24 hours incubation with the indicated concentrations of the CD3/MCSP “1+1 CrossMab”, “1+1 IgG Crossfab (N-terminal)” and “2+1 IgG Crossfab (N-terminal)” constructs. The assay was med in the presence or absence of MV-3 target cells, as indicated.

FIGURE 22. Killing (as measured by LDH release) of MKN-45 (A) or LS-174T (B) tumor cells upon co-culture with human PBMCs (E:T ratio = 10:1) and activation for 28 hours by different concentrations of the “2+1 IgG Crossfab (N-terminal), inverted (VL/VH)” versus the “2+1 IgG Crossfab (N-terminal), inverted 1)” construct.

FIGURE 23. Killing (as measured by LDH release) of WM266-4 tumor cells upon co-culture with human PBMCs (E:T ratio = 10:1) and activation for 26 hours by different concentrations of the “2+1 IgG Crossfab (N-terminal) (VL/VH)” versus the “2+1 IgG Crossfab minal) (CL/CH1)” construct.

FIGURE 24. Killing (as measured by LDH release) of MV-3 tumor cells upon co-culture with human PBMCs (E:T ratio = 10:1) and tion for 27 hours by different concentrations of the “2+1 IgG Crossfab (N-terminal) (VH/VL)” versus the “2+1 IgG Crossfab (N-terminal) (CL/CH1)” constructs.

FIGURE 25. Killing (as measured by LDH release) of human MCSP-positive WM266-4 (A) or MV-3 (B) tumor cells upon co-culture with human PBMCs (E:T ratio = 10:1) and activation for 21 hours by different concentrations of the “2+1 IgG Crossfab (N-terminal)”, the “1+1 CrossMab”, and the “1+1 IgG Crossfab (N-terminal)”, as indicated.

FIGURE 26. Binding of bispecific ucts to human CD3, expressed by Jurkat cells (A), or to human CEA, expressed by LS-174T cells (B) as determined by FACS. As a control, the lent maximum concentration of the nce IgGs and the background staining due to the labeled 2ndary antibody (goat anti-human FITC-conjugated AffiniPure F(ab’)2 Fragment, Fcγ Fragment-specific, Jackson Immuno Research Lab # 109098) were assessed as well.

FIGURE 27. Binding of bispecific ucts to human CD3, expressed by Jurkat cells, or to human MCSP, expressed by WM266-4 tumor cells (B) as determined by FACS.

Detailed Description of the ion Definitions Terms are used herein as generally used in the art, unless otherwise defined in the following.

As used herein, the term "antigen binding molecule" refers in its broadest sense to a molecule that specifically binds an antigenic determinant. Examples of antigen binding molecules are immunoglobulins and derivatives, e.g. fragments, thereof.

The term “bispecific” means that the antigen binding le is able to specifically bind to two distinct antigenic inants. Typically, a bispecific antigen binding molecule comprises two antigen binding sites, each of which is specific for a ent antigenic determinant. In certain embodiments the bispecific antigen binding molecule is capable of simultaneously binding two antigenic determinants, particularly two antigenic determinants expressed on two distinct cells.

As used herein, the term "antigenic determinant" is synonymous with "antigen" and "epitope," and refers to a site (e.g. a contiguous stretch of amino acids or a conformational configuration made up of different regions of non-contiguous amino acids) on a polypeptide macromolecule to which an antigen binding moiety binds, g an antigen binding moiety-antigen complex.

Useful antigenic inants can be found, for example, on the es of tumor cells, on the surfaces of virus-infected cells, on the surfaces of other diseased cells, on the surface of immune cells, free in blood serum, and/or in the extracellular matrix (ECM). The proteins referred to as antigens herein (e.g. MCSP, FAP, CEA, EGFR, CD33, CD3, c-Met, Her3) can be any native form the proteins from any vertebrate source, ing mammals such as primates (e.g. humans) and rodents (e.g. mice and rats), unless otherwise indicated. In a particular embodiment the antigen is a human protein. Where reference is made to a specific protein , the term encompasses the “full-length”, unprocessed protein as well as any form of the protein that results from processing in the cell. The term also encompasses naturally ing variants of the protein, e.g. splice variants or allelic variants. ary human proteins useful as ns include, but are not limited to: Melanoma-associated Chondroitin Sulfate glycan (MCSP), also known as oitin Sulfate Proteoglycan 4 (UniProt no. Q6UVK1, NCBI Accession no.

NP_001888); Fibroblast Activation n (FAP), also known as Seprase (Uni Prot nos. Q12884, Q86Z29, Q99998, NCBI Accession no. NP_004451); Carcinoembroynic antigen (CEA), also known as Carcinoembryonic antigen-related cell adhesion molecule 5 (UniProt no. P06731, NCBI Accession no. 354); CD33, also known as gp67 or Siglec-3 (UniProt no. P20138, NCBI Accession nos. NP_001076087, NP_001171079); Epidermal Growth Factor Receptor (EGFR), also known as ErbB-1 or Her1 (UniProt no. P0053, NCBI Accession nos. NP_958439, NP_958440), CD3, particularly the epsilon subunit of CD3 (UniProt no. P07766, NCBI Accession no. 724); c-Met, also known as cyte Growth Factor Receptor (UniProt no. P08581, NCBI Accession nos. NP_000236, NP_001120972) and Her3, also known as ErbB- 3 ot no. P21860, NCBI Accession nos. NP_001973, NP_001005915). In certain embodiments the bispecific antigen binding molecule described binds to an epitope of an first antigen or a second antigen that is conserved among the first antigen or second antigen from different species.

By "specific binding" is meant that the binding is selective for the n and can be discriminated from ed or non-specific interactions. The y of an antibody to bind to a specific antigenic determinant can be measured either through an -linked immunosorbent assay (ELISA) or other techniques familiar to one of skill in the art, e.g. surface plasmon resonance (SPR) technique (analyzed on a BIAcore ment) (Liljeblad et al., Glyco J 17, 323-329 (2000)), and traditional binding assays (Heeley, Endocr Res 28, 217-229 ). In one embodiment, the extent of binding of an antibody to an unrelated protein is less than about 10% of the binding of the antibody to the antigen as measured, e.g., by SPR. In certain embodiments, an antibody or a fragement thereof that binds to the n has a dissociation constant (KD) of ≤ 1 μM, ≤ 100 nM, ≤ 10 nM, ≤ 1 nM, ≤ 0.1 nM, ≤ 0.01 nM, or ≤ 0.001 nM (e.g. 10-8 M or less, e.g. from 10-8 M to 10-13 M, e.g., from 10-9 M to 10-13 M).

“Affinity” refers to the strength of the sum total of non-covalent interactions between a single binding site of a molecule (e.g., a receptor) and its binding partner (e.g., a ligand). Unless indicated otherwise, as used , “binding affinity” refers to intrinsic g affinity which reflects a 1:1 interaction between members of a binding pair (e.g., an antigen binding moiety and an n, or a receptor and its ). The affinity of a molecule X for its partner Y can generally be represented by the dissociation constant (KD), which is the ratio of iation and association rate constants (koff and kon, respectively). Thus, equivalent affinities may comprise different rate constants, as long as the ratio of the rate constants remains the same. Affinity can be measured by well established methods known in the art, including those described herein. A particular method for measuring affinity is Surface Plasmon Resonance (SPR).

The term “valent” as used herein denotes the presence of a specified number of antigen binding sites in an antigen binding molecule. As such, the term “monovalent binding to an antigen” denotes the presence of one (and not more than one) n binding site specific for the antigen in the antigen binding molecule.

An “antigen g site” refers to the site, i.e. one or more amino acid es, of an antigen binding molecule which provides interaction with the antigen. For example, the antigen binding site of an antibody comprises amino acid residues from the complementarity determining regions . A native globulin molecule typically has two antigen binding sites, a Fab nt typically has a single antigen binding site.

As used , the term "antigen g moiety" refers to a polypeptide molecule that specifically binds to an antigenic determinant. Antigen binding moieties e antibodies and fragments thereof as further defined herein. Particular antigen binding moieties include an n binding domain of an antibody, comprising an antibody heavy chain variable region and an antibody light chain variable region. In certain embodiments, the antigen binding moieties may comprise antibody constant regions as further defined herein and known in the art. Useful heavy chain constant regions include any of the five isotypes: α, δ, ε, γ, or μ. Useful light chain constant regions include any of the two isotypes: κ and λ.

As used herein, the terms ” and “second” with respect to Fab fragments etc., are used for ience of distinguishing when there is more than one of each type of moiety. Use of these terms is not intended to confer a specific order or orientation of the bispecific antigen binding molecule unless explicitly so stated.

As used herein, the term "single-chain" refers to a molecule sing amino acid rs linearly linked by peptide bonds. By a single-chain Fab fragment is meant a Fab molecule wherein the Fab light chain and the Fab heavy chain are connected by a peptide linker to form a single peptide chain.

The term “immunoglobulin molecule” refers to a protein having the structure of a naturally occurring antibody. For example, immunoglobulins of the IgG class are heterotetrameric glycoproteins of about 150,000 daltons, composed of two light chains and two heavy chains that are disulfide-bonded. From N- to C-terminus, each heavy chain has a variable region (VH), also called a variable heavy domain or a heavy chain variable domain, followed by a hinge region (HR) and three nt domains (CH1, CH2, and CH3), also called a heavy chain constant region. In case of an IgE class immunoglobulin the heavy chain additionally has a CH4 domain.

Hence, an immunoglobulin heavy chain is a polypeptide consisting in N-terminal to C-terminal direction of the following domains: VH-CH1-HR-CH2-CH3-(CH4). Similarly, from N- to C- terminus, each light chain has a variable region (VL), also called a variable light domain or a light chain variable , followed by a constant light (CL) domain, also called a light chain constant region. Hence, an immunoglobulin light chain is a polypeptide consisting in inal to C-terminal direction of the following domains: VL-CL. The heavy chain of an immunoglobulin may be assigned to one of five types, called α (IgA), δ (IgD), ε (IgE), γ (IgG), or μ (IgM), some of which may be further divided into es, e.g. γ1 (IgG1), γ2 (IgG2), γ3 (IgG3), γ4 (IgG4), α1 (IgA1) and α2 (IgA2). The light chain of an immunoglobulin may be assigned to one of two types, called kappa (κ) and lambda (λ), based on the amino acid sequence of its constant domain. An immunoglobulin essentially consists of two Fab fragments and an Fc domain, linked via the immunoglobulin hinge region.

The term "antibody" herein is used in the broadest sense and encompasses various antibody structures, including but not limited to monoclonal antibodies, polyclonal antibodies, and antibody fragments so long as they exhibit the desired n-binding activity.

An "antibody nt" refers to a molecule other than an intact antibody that comprises a portion of an intact antibody that binds the antigen to which the intact antibody binds. Examples of antibody nts include but are not limited to Fv, Fab, Fab', H, F(ab')2, diabodies, linear antibodies, -chain antibody molecules (e.g. scFv), and single-domain antibodies. For a review of certain antibody fragments, see Hudson et al., Nat Med 9, 129-134 (2003). For a review of scFv fragments, see e.g. Plückthun, in The cology of Monoclonal Antibodies, vol. 113, Rosenburg and Moore eds., Springer-Verlag, New York, pp. 269-315 (1994); see also WO 93/16185; and U.S. Patent Nos. 5,571,894 and 5,587,458. For discussion of Fab and F(ab')2 fragments comprising salvage receptor binding epitope residues and having increased in vivo half-life, see U.S. Patent No. 5,869,046. Diabodies are antibody fragments with two antigenbinding sites that may be bivalent or bispecific. See, for example, EP 404,097; WO 1161; Hudson et al., Nat Med 9, 129-134 ; and ger et al., Proc Natl Acad Sci USA 90, 6444-6448 (1993). dies and tetrabodies are also described in Hudson et al., Nat Med 9, 129-134 (2003). Single-domain antibodies are antibody fragments comprising all or a portion of the heavy chain variable domain or all or a portion of the light chain variable domain of an antibody. In certain embodiments, a single-domain antibody is a human single-domain antibody tis, Inc., Waltham, MA; see e.g. U.S. Patent No. 6,248,516 B1). Antibody fragments can be made by various techniques, including but not limited to proteolytic digestion of an intact antibody as well as production by recombinant host cells (e.g. E. coli or , as described herein.

A “Fab fragment” refers to a protein consisting of the VH and CH1 domain of the heavy chain (the “Fab heavy chain”) and the VL and CL domain of the light chain (the “Fab light chain”) of an immunoglobulin. A Fab fragment being fused to another protein is, in its unmodified form, fused at its heavy chain C- or N-terminus. Consequently, where the variable domains VH and VL are replaced by each other, the Fab fragment is fused at the C-terminus of the CH1 domain or the inus of the VL domain. Similarly, where the constant domains CH1 and CL are replaced by each other, the Fab fragment is fused at the inus of the CL domain or the N- terminus of the VH domain, and where the complete Fab heavy chain (VH-CH1) and Fab light chain (VL-CL) are replaced by each other, the Fab nt is fused at its light chain C- or N- terminus.

By “fused” is meant that the components (e.g. a Fab fragment and an Fc domain t) are linked by peptide bonds, either directly or via one or more peptide linkers.

The term "antigen g domain" refers to the part of an antibody that comprises the area which specifically binds to and is complementary to part or all of an antigen. An antigen binding domain may be ed by, for example, one or more dy variable domains (also called antibody variable regions). Particularly, an antigen g domain comprises an antibody light chain variable region (VL) and an antibody heavy chain variable region (VH).

The term “variable region” or “variable domain” refers to the domain of an antibody heavy or light chain that is involved in binding the antibody to antigen. The variable domains of the heavy chain and light chain (VH and VL, respectively) of a native antibody generally have similar ures, with each domain comprising four conserved framework regions (FRs) and three hypervariable regions (HVRs). See, e.g., Kindt et al., Kuby Immunology, 6th ed., W.H. Freeman and Co., page 91 (2007). A single VH or VL domain may be sufficient to confer antigen-binding icity.

The term variable region” or “HVR”, as used herein, refers to each of the regions of an antibody variable domain which are hypervariable in sequence and/or form structurally defined loops rvariable loops”). Generally, native four-chain antibodies comprise six HVRs; three in the VH (H1, H2, H3), and three in the VL (L1, L2, L3). HVRs generally comprise amino acid residues from the hypervariable loops and/or from the complementarity determining regions (CDRs), the latter being of highest sequence variability and/or involved in antigen recognition.

With the exception of CDR1 in VH, CDRs generally comprise the amino acid residues that form the hypervariable loops. Hypervariable regions (HVRs) are also referred to as “complementarity determining regions” (CDRs), and these terms are used herein interchangeably in reference to portions of the variable region that form the antigen g regions. This particular region has been described by Kabat et al., U.S. Dept. of Health and Human Services, Sequences of ns of Immunological st (1983) and by Chothia et al., J Mol Biol 196:901-917 (1987), where the definitions include overlapping or subsets of amino acid residues when compared t each other. Nevertheless, application of either definition to refer to a CDR of an antibody or variants thereof is intended to be within the scope of the term as defined and used herein. The appropriate amino acid residues which encompass the CDRs as defined by each of the above cited references are set forth below in Table 1 as a comparison. The exact residue numbers which encompass a ular CDR will vary depending on the sequence and size of the CDR. Those skilled in the art can routinely determine which residues se a particular CDR given the variable region amino acid sequence of the antibody.

TABLE 1. CDR Definitions1 CDR Kabat Chothia AbM2 VH CDR1 31-35 26-32 26-35 VH CDR2 50-65 52-58 50-58 VH CDR3 95-102 95-102 95-102 VL CDR1 24-34 26-32 24-34 VL CDR2 50-56 50-52 50-56 VL CDR3 89-97 91-96 89-97 1 ing of all CDR definitions in Table 1 is according to the numbering conventions set forth by Kabat et al. (see below). 2 "AbM" with a ase “b” as used in Table 1 refers to the CDRs as defined by Oxford Molecular's "AbM" antibody modeling software.

Kabat et al. also defined a ing system for variable region sequences that is applicable to any antibody. One of ordinary skill in the art can unambiguously assign this system of "Kabat numbering" to any variable region ce, without reliance on any experimental data beyond the sequence itself. As used herein, "Kabat numbering" refers to the numbering system set forth by Kabat et al., U.S. Dept. of Health and Human Services, "Sequence of Proteins of Immunological Interest" (1983). Unless otherwise specified, references to the ing of specific amino acid residue positions in an antibody variable region are according to the Kabat numbering system.

The polypeptide sequences of the ce listing are not numbered according to the Kabat numbering system. However, it is well within the ordinary skill of one in the art to convert the numbering of the sequences of the Sequence Listing to Kabat numbering.

"Framework" or "FR" refers to variable domain residues other than ariable region (HVR) residues. The FR of a variable domain generally consists of four FR domains: FR1, FR2, FR3, and FR4. Accordingly, the HVR and FR sequences generally appear in the following sequence in VH (or VL): FR1-H1(L1)-FR2-H2(L2)-FR3-H3(L3)-FR4.

The ” of an antibody or immunoglobulin refers to the type of nt domain or constant region possessed by its heavy chain. There are five major classes of antibodies: IgA, IgD, IgE, IgG, and IgM, and several of these may be further divided into subclasses (isotypes), e.g., IgG1, IgG2, IgG3, IgG4, IgA1, and IgA2. The heavy chain constant s that correspond to the different classes of immunoglobulins are called α, δ, ε, γ, and μ, respectively.

The term “Fc domain” or “Fc region” herein is used to define a C-terminal region of an immunoblobulin heavy chain that contains at least a portion of the constant region. The term includes native sequence Fc regions and variant Fc regions. Although the ries of the Fc region of an IgG heavy chain might vary slightly, the human IgG heavy chain Fc region is usually defined to extend from Cys226, or from , to the carboxyl-terminus of the heavy chain. However, the C-terminal lysine (Lys447) of the Fc region may or may not be present.

Unless otherwise specified , numbering of amino acid residues in the Fc region or constant region is ing to the EU numbering system, also called the EU index, as described in Kabat et al., ces of Proteins of Immunological Interest, 5th Ed. Public Health e, National Institutes of Health, Bethesda, MD, 1991. A “subunit” of an Fc domain as used herein refers to one of the two polypeptides forming the dimeric Fc domain, i.e. a polypeptide comprising C- terminal constant regions of an immunoglobulin heavy chain, e of stable self-association.

For example, a subunit of an IgG Fc domain comprises an IgG CH2 and an IgG CH3 constant region.

A “modification promoting the association of the first and the second subunit of the Fc domain” is a manipulation of the peptide ne or the post-translational modifications of an Fc domain subunit that reduces or prevents the association of a polypeptide comprising the Fc domain subunit with an identical polypeptide to form a homodimer. A modification promoting association as used herein particularly includes separate modifications made to each of the two Fc domain subunits desired to associate (i.e. the first and the second subunit of the Fc domain), wherein the modifications are complementary to each other so as to promote ation of the two Fc domain subunits. For example, a modification promoting association may alter the structure or charge of one or both of the Fc domain subunits so as to make their association sterically or electrostatically ble, respectively. Thus, (hetero)dimerization occurs between a polypeptide comprising the first Fc domain subunit and a polypeptide comprising the second Fc domain subunit, which might be non-identical in the sense that further components fused to each of the subunits (e.g. Fab fragments) are not the same. In some embodiments the modification promoting association comprises an amino acid mutation in the Fc domain, specifically an amino acid substitution. In a particular embodiment, the modification ing association comprises a separate amino acid mutation, specifically an amino acid substitution, in each of the two subunits of the Fc domain.

The term tor functions” refers to those biological ties attributable to the Fc region of an antibody, which vary with the antibody isotype. Examples of antibody effector functions include: C1q binding and complement dependent cytotoxicity (CDC), Fc receptor binding, antibody-dependent cell-mediated cytotoxicity (ADCC), antibody-dependent cellular phagocytosis (ADCP), cytokine secretion, immune complex-mediated antigen uptake by antigen presenting cells, down regulation of cell surface receptors (e.g. B cell receptor), and B cell activation.

As used herein, the terms eer, engineered, engineering”, are considered to include any manipulation of the peptide backbone or the post-translational modifications of a naturally ing or recombinant polypeptide or fragment thereof. Engineering es modifications of the amino acid sequence, of the glycosylation pattern, or of the side chain group of individual amino acids, as well as combinations of these approaches.

The term “amino acid mutation” as used herein is meant to encompass amino acid substitutions, deletions, insertions, and cations. Any combination of tution, deletion, insertion, and modification can be made to arrive at the final construct, provided that the final construct possesses the desired characteristics, e.g., reduced binding to an Fc receptor, or increased association with r e. Amino acid sequence deletions and ions include aminoand /or carboxy-terminal deletions and insertions of amino acids. ular amino acid mutations are amino acid substitutions. For the purpose of ng e.g. the binding characteristics of an Fc region, non-conservative amino acid substitutions, i.e. replacing one amino acid with another amino acid having different structural and/or chemical properties, are particularly preferred.

Amino acid substitutions include replacement by non-naturally occurring amino acids or by naturally occurring amino acid tives of the twenty standard amino acids (e.g. 4- hydroxyproline, 3-methylhistidine, ornithine, rine, oxylysine). Amino acid mutations can be generated using genetic or chemical s well known in the art. Genetic methods may include site-directed mutagenesis, PCR, gene synthesis and the like. It is plated that methods of altering the side chain group of an amino acid by methods other than genetic engineering, such as chemical modification, may also be useful. Various designations may be used herein to indicate the same amino acid mutation. For example, a substitution from e at position 329 of the Fc domain to glycine can be indicated as 329G, G329, G329, P329G, or Pro329Gly.

As used herein, term "polypeptide" refers to a molecule composed of monomers (amino acids) linearly linked by amide bonds (also known as e bonds). The term "polypeptide" refers to any chain of two or more amino acids, and does not refer to a specific length of the product.

Thus, es, dipeptides, tripeptides, oligopeptides, "protein," "amino acid chain," or any other term used to refer to a chain of two or more amino acids, are included within the definition of "polypeptide," and the term "polypeptide" may be used instead of, or interchangeably with any of these terms. The term "polypeptide" is also ed to refer to the products of post-expression modifications of the polypeptide, including without tion glycosylation, acetylation, phosphorylation, amidation, derivatization by known protecting/blocking groups, proteolytic cleavage, or modification by non-naturally occurring amino acids. A ptide may be derived from a l ical source or produced by recombinant technology, but is not necessarily translated from a designated nucleic acid sequence. It may be generated in any manner, including by al synthesis. A polypeptide described may be of a size of about 3 or more, 5 or more, or more, 20 or more, 25 or more, 50 or more, 75 or more, 100 or more, 200 or more, 500 or more, 1,000 or more, or 2,000 or more amino acids. Polypeptides may have a defined threedimensional structure, although they do not necessarily have such structure. Polypeptides with a defined three-dimensional structure are referred to as folded, and polypeptides which do not possess a defined three-dimensional structure, but rather can adopt a large number of different conformations, and are referred to as unfolded.

By an "isolated" polypeptide or a variant, or derivative thereof is intended a polypeptide that is not in its natural milieu. No particular level of purification is required. For example, an isolated ptide can be removed from its native or natural environment. Recombinantly produced polypeptides and proteins expressed in host cells are ered isolated for the purpose description, as are native or recombinant polypeptides which have been separated, fractionated, or partially or substantially purified by any le technique.

By "isolated" nucleic acid molecule or polynucleotide is intended a c acid molecule, DNA or RNA, which has been removed from its native environment. For e, a recombinant polynucleotide encoding a polypeptide contained in a vector is considered isolated for the purposes of the present description. r examples of an isolated polynucleotide include recombinant polynucleotides maintained in heterologous host cells or purified (partially or substantially) polynucleotides in solution. An isolated polynucleotide es a polynucleotide molecule contained in cells that ordinarily contain the polynucleotide molecule, but the polynucleotide molecule is present extrachromosomally or at a chromosomal location that is different from its natural chromosomal location. Isolated RNA molecules include in vivo or in vitro RNA transcripts, as well as positive and negative strand forms, and double-stranded forms.

Isolated polynucleotides or nucleic acids according to the t description further include such molecules ed synthetically. In addition, a polynucleotide or a nucleic acid may be or may include a regulatory element such as a promoter, ribosome binding site, or a transcription terminator.

The term “vector” or ssion " is synonymous with "expression construct" and refers to a DNA molecule that is used to introduce and direct the expression of a specific gene to which it is operably associated in a target cell. The term es the vector as a self-replicating nucleic acid ure as well as the vector incorporated into the genome of a host cell into which it has been introduced. The expression vector described comprises an expression cassette. Expression vectors allow transcription of large amounts of stable mRNA. Once the expression vector is inside the target cell, the ribonucleic acid molecule or protein that is encoded by the gene is produced by the ar transcription and/or translation ery. In one embodiment, the expression vector described comprises an expression cassette that comprises polynucleotide sequences that encode bispecific antigen binding molecules described or fragments thereof.

The terms "host cell", "host cell line," and "host cell culture" are used interchangeably and refer to cells into which exogenous nucleic acid has been introduced, including the progeny of such cells. Host cells include "transformants" and "transformed " which e the primary transformed cell and progeny derived therefrom without regard to the number of passages.

Progeny may not be completely identical in nucleic acid content to a parent cell, but may contain mutations. Mutant progeny that have the same function or biological activity as screened or selected for in the originally transformed cell are included . A host cell is any type of cellular system that can be used to generate the bispecific antigen binding molecules described.

Host cells include cultured cells, e.g. mammalian cultured cells, such as CHO cells, BHK cells, NS0 cells, SP2/0 cells, YO a cells, P3X63 mouse myeloma cells, PER cells, PER.C6 cells or hybridoma cells, yeast cells, insect cells, and plant cells, to name only a few, but also cells sed within a transgenic animal, transgenic plant or ed plant or animal tissue.

An “activating Fc receptor” is an Fc receptor that following engagement by an Fc domain of an antibody elicits ing events that stimulate the receptor-bearing cell to perform effector functions. Human activating Fc receptors include FcγRIIIa ), FcγRI (CD64), FcγRIIa (CD32), and FcαRI (CD89).

Antibody-dependent cell-mediated cytotoxicity (ADCC) is an immune mechanism leading to the lysis of antibody-coated target cells by immune effector cells. The target cells are cells to which antibodies or tives thereof comprising an Fc region specifically bind, generally via the protein part that is N-terminal to the Fc region. As used herein, the term “reduced (or sed) ADCC” is defined as either a reduction (increase) in the number of target cells that are lysed in a given time, at a given concentration of antibody in the medium surrounding the target cells, by the mechanism of ADCC defined above, and/or an increase (reduction) in the concentration of antibody in the medium surrounding the target cells, ed to achieve the lysis of a given number of target cells in a given time, by the mechanism of ADCC. The reduction (increase) in ADCC is relative to the ADCC mediated by the same antibody produced by the same type of host cells, using the same standard production, purification, formulation and storage s (which are known to those skilled in the art), but that has not been engineered. For example the reduction in ADCC mediated by an antibody comprising in its Fc domain an amino acid substitution that s ADCC, is relative to the ADCC mediated by the same antibody without this amino acid substitution in the Fc domain. Suitable assays to measure ADCC are well known in the art (see e.g. PCT publication no. or PCT patent application no.

An "effective amount" of an agent refers to the amount that is necessary to result in a physiological change in the cell or tissue to which it is administered.

A "therapeutically effective amount" of an agent, e.g. a pharmaceutical composition, refers to an amount effective, at dosages and for periods of time necessary, to achieve the d therapeutic or prophylactic result. A therapeutically effective amount of an agent for example eliminates, decreases, delays, minimizes or prevents adverse effects of a disease.

An “individual” or “subject” is a mammal. Mammals include, but are not limited to, domesticated animals (e.g. cows, sheep, cats, dogs, and ), primates (e.g. humans and non- human primates such as monkeys), rabbits, and rodents (e.g. mice and rats). Particularly, the individual or subject is a human.

The term "pharmaceutical composition" refers to a preparation which is in such form as to permit the biological ty of an active ient contained therein to be effective, and which contains no additional components which are unacceptably toxic to a subject to which the ation would be stered.

A “pharmaceutically acceptable carrier” refers to an ingredient in a pharmaceutical composition, other than an active ingredient, which is ic to a subject. A pharmaceutically acceptable carrier includes, but is not limited to, a buffer, excipient, stabilizer, or preservative.

As used herein, “treatment” (and tical variations thereof such as “treat” or “treating”) refers to clinical intervention in an attempt to alter the natural course of a e in the individual being treated, and can be performed either for prophylaxis or during the course of clinical pathology. Desirable effects of treatment include, but are not limited to, preventing occurrence or recurrence of disease, ation of ms, diminishment of any direct or indirect pathological consequences of the disease, ting metastasis, decreasing the rate of disease ssion, amelioration or palliation of the disease state, and ion or improved prognosis. In some embodiments, bispecific antigen binding molecules described are used to delay development of a disease or to slow the progression of a disease.

The term “package insert” is used to refer to instructions customarily included in commercial packages of therapeutic products, that contain information about the indications, usage, dosage, stration, combination therapy, contraindications and/or warnings ning the use of such therapeutic products.

The term “comprising” as used in this specification means “consisting at least in part of”. When interpreting each statement in this specification that includes the term “comprising”, features other than that or those prefaced by the term may also be present. Related terms such as “comprise” and “comprises” are to be interpreted in the same manner.

Detailed Description of the ments Described is a bispecific antigen binding le, comprising a first Fab fragment which specifically binds to a first antigen, a second Fab fragment which specifically binds to a second antigen, and an Fc domain composed of a first and a second subunit capable of stable association; wherein a) the bispecific antigen binding molecule provides monovalent binding to the first and/or the second antigen, b) the first Fab nt, the second Fab fragment and the first Fc domain subunit are fused to each other, and c) in the first and/or the second Fab fragment one of the following replacements is made: (i) the variable domains VL and VH are replaced by each other, (ii) the constant domains CL and CH1 are replaced by each other, or (iii) both the le and constant s VL-CL and VH-CH1 are replaced by each other, provided that not the same replacement is made in the first and the second Fab nt.

Bispecific antigen binding le formats The components of the bispecific antigen binding molecule can be fused to each other in a variety of configurations. Exemplary configurations are depicted in Figure 1.

In ular embodiments, the first Fab fragment is fused at its C-terminus to the N-terminus of the second Fab fragment, which is in turn fused at its C-terminus to the N-terminus of the first Fc domain subunit (see examples in Figure 1A and 1B). In one such embodiment, the second Fab fragment is fused to the first Fc domain t via an immunoglobulin hinge region. In a further such embodiment, the first Fab nt is fused to the second Fab fragment via a peptide linker.

In one embodiment, the first Fab fragment is fused at the C-terminus of its heavy chain to the N- terminus of the heavy chain of the second Fab fragment, which is in turn fused at the C-terminus of its heavy chain to the N-terminus of the first Fc domain subunit.

In other embodiments, the second Fab fragment is fused at its C-terminus to the N-terminus of the first Fab nt, which is in turn fused at its C-terminus to the N-terminus of the first Fc domain subunit (see example in Figure 1C). In one such embodiment, the first Fab fragment is fused to the first Fc domain subunit via an immunoglobulin hinge region. In a further such embodiment, the second Fab fragment is fused to the first Fab fragment via a peptide linker. In one embodiment, the second Fab fragment is fused at the C-terminus of its heavy chain to the N- terminus of the heavy chain of the first Fab fragment, which is in turn fused at the C-terminus of its heavy chain to the N-terminus of the first Fc domain subunit.

In some embodiments wherein either the first Fab fragment is fused at the C-terminus of its heavy chain to the N-terminus of the heavy chain of the second Fab fragment which is in turn fused at the C-terminus of its heavy chain to the inus of the first Fc domain t, or the second Fab fragment is fused at the inus of its heavy chain to the N-terminus of the heavy chain of the first Fab fragment which is in turn fused at the C-terminus of its heavy chain to the N-terminus of the first Fc domain subunit, additionally the Fab light chain of the first Fab fragment and the Fab light chain of the second Fab fragment are fused to each other, optionally via a peptide linker (see examples in Figure 12).

According to these embodiments, two Fab fragments of ent specificity are fused to each other, one of which is in turn fused to an Fc domain subunit. This configuration allows for a geometry (e.g. ce, angle between the Fab nts) different from the cal bispecific immunoglobulin format with the two Fab fragments of the immunoglobulin molecule having different specificities. For example, the ors found that this configuration is more suitable than the classical bispecific immunoglobulin format for mimicking an immunological synapse between a T cell and a target cell, as required if the bispecific antigen binding molecule is to be used for T cell engagement and re-direction (data not shown).

In other embodiments, the second Fab fragment is fused at its C-terminus to the inus of the first Fc domain subunit, which is in turn fused at its C-terminus to the N-terminus of the first Fab nt (see example in Figure 1D). In one such embodiment, the second Fab fragment is fused to the first Fc domain subunit via an immunoglobulin hinge region. In a further such embodiment, the first Fab fragment is fused to the first Fc domain subunit via a peptide linker. In one embodiment, the second Fab fragment is fused at the inus of its heavy chain to the N- terminus of the first Fc domain subunit, which is in turn fused at its C-terminus to the N- terminus of the heavy chain of the first Fab fragment. According to these embodiments, two Fab fragments of different specificity are fused to the two termini of an Fc domain subunit. Again, this configuration allows for a distinct geometry which might be advantageous for particular applications. In one embodiment the bispecific antigen binding molecule essentially consists of the first Fab fragment, the second Fab fragment, the Fc domain, and optionally one or more peptide linkers.

The bispecific antigen binding molecule described provides monovalent binding to at least one of the two antigens it binds to. Monovalent binding is important, for example in cases where internalization of the target antigen is to be expected ing binding of a high affinity n binding molecule. In such cases, the presence of more than one antigen g moiety specific for the target antigen may enhance internalization of the antigen, y reducing its availablity.

Furthermore, lent binding is essential where crosslinking of target antigen is not desired.

For example in bispecific antigen binding les for T cell engagement and re-direction, bivalent binding to an activating T cell antigen such as CD3 could lead to activation of the T cell even in the absence of target cells.

In other cases, however, bivalent binding might be desirable, for example to increase binding affinity, optimize ing to the target site or allow inking of a target antigen.

Accordingly, in particular embodiments, the bispecific antigen binding molecule comprises a third Fab fragment which specifically binds to the first or the second antigen. In one embodiment, the third Fab fragment is fused to the second Fc domain subunit. In a more specific embodiment, the third Fab fragment is fused at its C-terminus to the N-terminus of the second Fc domain subunit. In an even more specific embodiment, the third Fab fragment is fused at the C-terminus of its heavy chain to the inus of the second Fc domain t. In one embodiment, the third Fab fragment is fused to the second Fc domain subunit via an immunoglobulin hinge region.

In one embodiment, the third Fab fragment ically binds to the second antigen.

In some embodiments the second Fab fragment, the third Fab fragment and the Fc domain are part of an immunoglobulin molecule. In embodiments where the third Fab fragment specifically binds to the second antigen, the immunoglobulin molecule is an immunoglobulin le which specifically binds to the second antigen. In a specific such embodiment, the immunoglobulin molecule is an IgG class immunoglobulin molecule, more specifically an IgG1 or IgG4 ss immunoglobulin molecule. In one specific embodiment the immunoglobulin molecule is an IgG4 molecule comprising an amino acid substitution at position S228 (EU numbering), particularly the amino acid substitution S228P. This amino acid substitution reduces in vivo Fab arm exchange of IgG4 antibodies (see Stubenrauch et al., Drug Metabolism and Disposition 38, 84-91 (2010)). In one embodiment, the immunoglobulin le is a human immunoglobulin molecule. In one embodiment, the bispecific antigen binding molecule essentially consists of a first Fab fragment which specifically binds to the first antigen, an immunoglobulin molecule which specifically binds to the second antigen, and optionally one or more peptide linkers.

According to some of the above embodiments, the light chain of the first Fab fragment and the light chain of the second Fab fragment are fused to each other, optionally via a peptide linker.

Depending on the configuration of the first and the second Fab fragment, the light chain of the first Fab fragment may be fused at its C-terminus to the N-terminus of the light chain of the second Fab fragment, or the light chain of the second Fab fragment may be fused at its C- terminus to the N-terminus of the light chain of the first Fab fragment. Fusion of the light chains of the first and the second Fab fragment further reduces mispairing of unmatched Fab heavy and light chains, and also reduces the number of plasmids needed for expression of some of the bispecific antigen binding molecules described.

According to any of the above embodiments, components of the bispecific antigen binding molecule (e.g. Fab fragments, Fc domain subunit) may be linked ly or through various linkers, particularly peptide linkers comprising one or more amino acids, typically about 2-20 amino acids, that are described herein or are known in the art. Suitable, munogenic peptide linker include, for example, (G4S)n, (SG4)n, (G4S)n or G4(SG4)n peptide linkers, wherein n is generally a number between 1 and 10, typically between 2 and 4. A particularly le peptide linker for fusing the light chains of the first and the second Fab fragment to each other is (G4S)2. Additionally, peptide linkers may comprise (a portion of) an immunoglobulin hinge region. An exemplary such linker is EPKSC(D)-(G4S)2 (SEQ ID NOs 72 and 73). Particularly where a Fab fragment is linked to the N-terminus of an Fc domain subunit, it may be linked via an globulin hinge region or a portion thereof, with or t an onal peptide linker.

In certain embodiments the bispecific antigen g molecule comprises a polypeptide wherein a VL region shares a carboxy-terminal peptide bond with a CH1 region, which in turn shares a carboxy-terminal peptide bond with a peptide , which in turn shares a carboxy-terminal peptide bond with an immunoglobulin heavy chain (VH-CH1-HR-CH2-CH3-(CH4)). In some of these embodiments, the ific antigen binding molecule further ses an antibody light chain (VL-CL) and/or a ptide wherein a VH region shares a carboxy al peptide bond with a CL region. In some of these embodiments, the ific antigen binding le further comprises a polypeptide wherein a VH region shares a carboxy-terminal peptide bond with a CL region, which in turn shares a carboxy-terminal peptide bond with a peptide , which in turn shares a carboxy-terminal peptide bond with a Fab light chain (VL-CL).

In other embodiments the bispecific antigen binding molecule comprises a ptide wherein a Fab heavy chain 1) shares a carboxy-terminal peptide bond with a peptide , which in turn shares a carboxy-terminal peptide bond with a VL region, which in turn shares a carboxyterminal peptide bond with a CH1 region, which in turn shares a carboxy-terminal peptide bond with an Fc domain subunit ing an immunoglobulin hinge region (HR-CH2-CH3-(CH4)).

In some of these embodiments, the bispecific antigen binding molecule further ses an antibody light chain (VL-CL) and/or a polypeptide wherein a VH region shares a carboxy terminal e bond with a CL region. In some of these embodiments, the bispecific antigen binding molecule further comprises a polypeptide wherein a Fab light chain (VL-CL) shares a carboxy-terminal peptide bond with a peptide linker, which in turn shares a carboxy-terminal peptide bond with a VH region, which in turn shares a carboxy-terminal peptide bond with a CL region.

In certain embodiments the bispecific antigen binding molecule comprises a polypeptide wherein a VH region shares a y-terminal peptide bond with a CL region, which in turn shares a carboxy-terminal peptide bond with a peptide linker, which in turn shares a carboxy-terminal peptide bond with an immunoglobulin heavy chain (VH-CH1-HR-CH2-CH3-(CH4)). In some of these embodiments, the bispecific antigen binding molecule r comprises an antibody light chain (VL-CL) and/or a polypeptide n a VL region shares a y terminal peptide bond with a CH1 region. In some of these embodiments, the bispecific n g molecule further comprises a polypeptide wherein a VL region shares a carboxy-terminal peptide bond with a CH1 region, which in turn shares a carboxy-terminal peptide bond with a e linker, which in turn shares a carboxy-terminal peptide bond with a Fab light chain (VL-CL).

In other embodiments the bispecific antigen binding molecule comprises a ptide wherein a Fab heavy chain (VH-CH1) shares a carboxy-terminal peptide bond with a peptide linker, which in turn shares a carboxy-terminal peptide bond with a VH region, which in turn shares a carboxyterminal peptide bond with a CL region, which in turn shares a carboxy-terminal peptide bond with an Fc domain subunit ing an immunoglobulin hinge region (HR-CH2-CH3-(CH4)).

In some of these embodiments, the bispecific antigen binding molecule further comprises an antibody light chain (VL-CL) and/or a polypeptide wherein a VL region shares a carboxy terminal peptide bond with a CH1 region. In some of these embodiments, the bispecific antigen binding molecule further ses a polypeptide wherein a Fab light chain (VL-CL) shares a carboxy-terminal e bond with a peptide linker, which in turn shares a carboxy-terminal peptide bond with a VL region, which in turn shares a y-terminal e bond with a CH1 region.

In still other embodiments, the ific antigen g molecule comprises a ptide wherein an immunoglobulin heavy chain ((VH-CH1-HR-CH2-CH3-(CH4)) shares a carboxyterminal peptide bond with a peptide linker, which in turn shares a carboxy-terminal peptide bond with a VH region, which in turn shares a carboxy-terminal peptide bond with a CL region.

In one embodiment, the bispecific antigen binding molecule further comprises an immunoglobulin heavy chain ((VH-CH1-HR-CH2-CH3-(CH4)). In another embodiment, the bispecific antigen binding molecule further comprises an Fc domain subunit, optionally including an antibody hinge region ((HR)-CH2-CH3-(CH4)). In some of these embodiments, the ific antigen binding molecule further comprises an antibody light chain (VL-CL) and/or a polypeptide n a VL region shares a carboxy al peptide bond with a CH1 region.

Fab fragments The antigen binding molecule described is bispecific, i.e. it comprises at least two antigen binding moieties capable of specific binding to two distinct antigenic determinants. In a particular embodiment, the bispecific antigen binding le is e of simultaneous binding to two ct antigenic determinants. According to thedescription, the antigen binding moieties are Fab fragments (i.e. antigen binding s composed of a heavy and a light chain, each comprising a variable and a constant ). In one embodiment said Fab fragments are human. In r embodiment said Fab fragments are humanized. In yet another embodiment said Fab fragments comprise human heavy and light chain constant regions.

According to thedescription, at least one of the Fab fragments is a “Crossfab” fragment, wherein the variable and/or constant domains of the Fab heavy and light chain are exchanged. Such modifications prevent mispairing of heavy and light chains from different Fab fragments, thereby improving the yield and purity of the bispecific antigen binding molecule described in recombinant production. In other words, the problem of heavy and light chain mispairing in bispecific antibody production is overcome by the exchange of heavy and light chain variable and/or constant domains within one or more Fab fragments of the bispecific antigen binding le, so that Fab fragments of different icity do not have identical domain ement and uently do not “interchange” light chains.

Possible replacements include the following: (i) the variable domains of the Fab heavy and light chain (VH and VL) are replaced by each other; (ii) the constant domains of the Fab heavy and light chain (CH1 and CL) are replaced by each other; or (iii) the Fab heavy and light chain (VHCH1 and VL-CL) are replaced by each other.

To achieve the desired result, i.e. prevention of mispairing of heavy and light chains of different specificity, not the same replacement must be made in Fab fragments of different specificity. For example, in a Fab fragment which specifically binds to a first antigen, the heavy and light chain variable domains may be exchanged, while in a Fab fragment which specifically binds to a second antigen, the heavy and light chain constant region may be exchanged. As another e, in a Fab fragment which specifically binds to a first antigen, no replacement may be made, while in a Fab nt which specifically binds to a second antigen, the heavy and light chain variable domains may be exchanged.

In a particular embodiment, the same replacement is made in Fab fragments of the same specificity (i.e. in Fab fragments which specifically bind to the same antigen). A replacement need not be made in all Fab fragments comprised in the bispecific n binding molecule. For example in embodiments wherein there are three Fab fragments, it is sufficient to make a replacement only in the Fab fragment having a different specificity from the other two Fab fragments. Specifically, in embodiments wherein the bispecific antigen binding molecule comprises a third Fab fragment which binds to the first antigen, a replacement is made only in the second Fab fragment. rly, in embodiments wherein the bispecific antigen binding molecule comprises a third Fab nt which binds to the second antigen, a replacement is made only in the first Fab fragment.

In particular ments, a replacement is made in the first Fab nt. In one such embodiment, no further replacement is made. In some embodiments, the replacement is a replacement of the variable domains VL and VH by each other. In other embodiments the replacement is a ement of the constant domains CL and CH1 by each other. In still other embodiments, the replacement is a replacement of both the variable and constant domains VL- CL and VH-CH1 by each other.

In a particular embodiment, the bispecific antigen binding molecule es monovalent binding to the first antigen. In one embodiment, the bispecific antigen binding le does not comprise a single chain Fab fragment.

Described is a bispecific n binding le comprising a first Fab fragment which specifically binds to a first antigen, a second Fab fragment which specifically binds to a second antigen, and an Fc domain composed of a first and a second t capable of stable association; wherein a) the ific antigen binding molecule provides monovalent g to the first antigen, b) the first Fab fragment is fused at its C-terminus to the N-terminus of the second Fab fragment, which is in turn fused at its C-terminus to the N-terminus of the first Fc domain subunit, c) in the first Fab fragment the constant s CL and CH1 are replaced by each other, and d) the bispecific antigen binding molecule optionally comprises a third Fab fragment which specifically binds to the second antigen and is fused at its C-terminus to the N-terminus of the second Fc domain subunit.

Also described is a bispecific antigen binding molecule comprising a first Fab fragment which specifically binds to a first antigen, a second Fab fragment which specifically binds to a second antigen, and an Fc domain composed of a first and a second subunit capable of stable association; wherein a) the bispecific antigen binding le provides monovalent binding to the first antigen, b) the first Fab fragment is fused at its C-terminus to the inus of the second Fab fragment, which is in turn fused at its C-terminus to the inus of the first Fc domain subunit, c) in the first Fab fragment the variable domains VL and VH are replaced by each other, and d) the bispecific antigen binding molecule optionally comprises a third Fab fragment which specifically binds to the second antigen and is fused at its C-terminus to the N-terminus of the second Fc domain t.

Also described is a bispecific antigen binding le, comprising a first Fab fragment which specifically binds to a first antigen, a second Fab fragment which specifically binds to a second antigen, and an Fc domain composed of a first and a second subunit capable of stable association; wherein a) the bispecific antigen binding molecule provides monovalent binding to the first antigen, b) the second Fab fragment is fused at its C-terminus to the N-terminus of the first Fab fragment, which is in turn fused at its C-terminus to the N-terminus of the first Fc domain subunit, c) in the first Fab fragment the constant domains CL and CH1 are replaced by each other, and d) the bispecific antigen binding molecule optionally comprises a third Fab fragment which specifically binds to the second n and is fused at its C-terminus to the N-terminus of the second Fc domain subunit.

Also described is a bispecific antigen binding molecule, comprising a first Fab fragment which specifically binds to a first antigen, a second Fab fragment which specifically binds to a second antigen, and an Fc domain composed of a first and a second subunit capable of stable association; n a) the bispecific antigen binding le provides lent binding to the first antigen, b) the second Fab fragment is fused at its C-terminus to the N-terminus of the first Fab fragment, which is in turn fused at its C-terminus to the N-terminus of the first Fc domain subunit, c) in the first Fab fragment the variable domains VL and VH are replaced by each other, and d) the bispecific antigen binding molecule optionally ses a third Fab fragment which specifically binds to the second n and is fused at its C-terminus to the N-terminus of the second Fc domain subunit.

Also bed is a bispecific antigen binding molecule, comprising a first Fab fragment which specifically binds to a first antigen, a second Fab fragment which ically binds to a second antigen, and an Fc domain composed of a first and a second subunit capable of stable association; wherein a) the bispecific antigen binding molecule provides monovalent g to the first antigen, b) the second Fab fragment is fused at its C-terminus to the N-terminus of the first Fc domain subunit, which is in turn fused at its C-terminus to the N-terminus of the first Fab nt, c) in the first Fab fragment the constant domains CL and CH1 are replaced by each other, and d) the ific antigen binding molecule optionally comprises a third Fab fragment which specifically binds to the second antigen and is fused at its C-terminus to the N-terminus of the second Fc domain subunit.

Fc domain The Fc domain of the bispecific antigen binding le ts of a pair of polypeptide chains comprising heavy chain domains of an antibody le. For example, the Fc domain of an immunoglobulin G (IgG) le is a dimer, each subunit of which comprises the CH2 and CH3 IgG heavy chain constant domains. The two subunits of the Fc domain are capable of stable association with each other. The bispecific antigen binding molecule described comprises not more than one Fc domain.

In one embodiment described the Fc domain of the bispecific antigen binding molecule is an IgG Fc domain. In a particular embodiment the Fc domain is an IgG1 Fc domain. In another embodiment the Fc domain is an IgG4 Fc domain. In a more specific embodiment, the Fc domain is an IgG4 Fc domain comprising an amino acid tution at position S228 (EU ing), particularly the amino acid substitution S228P. This amino acid substitution reduces in vivo Fab arm exchange of IgG4 antibodies (see Stubenrauch et al., Drug Metabolism and Disposition 38, 84-91 (2010)). In a further particular embodiment the Fc domain is human. An exemplary ce of a human IgG1 Fc region is given in SEQ ID NO: 71.

Fc domain modifications promoting heterodimerization Bispecific antigen g molecules described comprise different Fab fragments, fused to one or the other of the two subunits of the Fc domain, thus the two subunits of the Fc domain are typically sed in two non-identical polypeptide chains. Recombinant co-expression of these polypeptides and subsequent dimerization leads to several possible combinations of the two polypeptides. To improve the yield and purity of bispecific antigen binding molecules in recombinant production, it will thus be ageous to introduce in the Fc domain of the bispecific n binding molecule a modification promoting the association of the desired polypeptides.

Accordingly, in particular embodiments, the Fc domain comprises a modification promoting the association of the first and the second Fc domain subunit. A modification may be present in the first Fc domain subunit and/or the second Fc domain subunit.

The site of most extensive protein-protein interaction between the two subunits of a human IgG Fc domain is in the CH3 domain of the Fc domain. Thus, in one embodiment said modification is in the CH3 domain of the Fc domain.

In a specific embodiment said modification is a so-called “knob-into-hole” modification, comprising a “knob” modification in one of the two ts of the Fc domain and a “hole” modification in the other one of the two subunits of the Fc domain.

The knob-into-hole technology is described e.g. in US 5,731,168; US 7,695,936; Ridgway et al., Prot Eng 9, 617-621 (1996) and Carter, J Immunol Meth 248, 7-15 (2001). Generally, the method involves introducing a protuberance (“knob”) at the ace of a first polypeptide and a corresponding cavity (“hole”) in the interface of a second polypeptide, such that the protuberance can be positioned in the cavity so as to promote heterodimer formation and hinder mer formation. Protuberances are constructed by replacing small amino acid side chains from the interface of the first polypeptide with larger side chains (e.g. ne or tryptophan).

Compensatory cavities of cal or similar size to the protuberances are created in the interface of the second polypeptide by replacing large amino acid side chains with r ones (e.g. alanine or threonine).

Accordingly, in a particular ment, in the CH3 domain of the first Fc domain subunit of the bispecific n binding molecule an amino acid e is replaced with an amino acid residue having a larger side chain volume, thereby ting a protuberance within the CH3 domain of the first t which is positionable in a cavity within the CH3 domain of the second subunit, and in the CH3 domain of the second Fc domain subunit an amino acid residue is replaced with an amino acid e having a smaller side chain volume, thereby generating a cavity within the CH3 domain of the second subunit within which the protuberance within the CH3 domain of the first subunit is positionable.

The protuberance and cavity can be made by altering the nucleic acid encoding the polypeptides, e.g. by site-specific mutagenesis, or by e synthesis.

In a specific embodiment, in the CH3 domain of the first subunit of the Fc domain the threonine residue at position 366 is replaced with a tryptophan residue (T366W), and in the CH3 domain of the second subunit of the Fc domain the tyrosine residue at position 407 is replaced with a valine residue (Y407V). In one embodiment, in the second subunit of the Fc domain additionally the ine residue at position 366 is replaced with a serine residue (T366S) and the leucine residue at position 368 is replaced with an alanine residue (L368A).

In yet a further embodiment, in the first subunit of the Fc domain additionally the serine residue at position 354 is replaced with a cysteine residue (S354C), and in the second subunit of the Fc domain additionally the tyrosine residue at position 349 is replaced by a cysteine residue (Y349C). Introduction of these two cysteine residues results in formation of a disulfide bridge between the two subunits of the Fc domain, r stabilizing the dimer (Carter, J Immunol Methods 248, 7-15 (2001)).

In an alternative embodiment a modification promoting association of the first and the second subunit of the Fc domain comprises a modification mediating electrostatic ng effects, e.g. as described in PCT publication . Generally, this method involves replacement of one or more amino acid residues at the interface of the two Fc domain subunits by charged amino acid residues so that homodimer formation becomes ostatically unfavorable but heterodimerization electrostatically favorable.

Fc domain modifications altering Fc receptor g and/or effector function In certain embodiments, the Fc domain is engineered to have altered binding affinity to an Fc receptor and/or altered effector function, as compared to a non-engineered Fc domain.

Binding to Fc receptors can be easily determined e.g. by ELISA, or by Surface Plasmon Resonance (SPR) using standard instrumentation such as a BIAcore instrument (GE Healthcare), and Fc receptors such as may be obtained by recombinant sion. A suitable such binding assay is described herein. atively, g affinity of Fc domains or bispecific antigen binding molecules comprising an Fc domain for Fc receptors may be evaluated using cell lines known to s particular Fc receptors, such as NK cells expressing FcγIIIa receptor.

Effector function of an Fc domain, or a bispecific n g molecule comprising an Fc , can be measured by methods known in the art. Suitable in vitro assays to assess ADCC ty of a le of interest are described in PCT publication no. or PCT patent application no. , orated herein by reference in their entirety.

Useful effector cells for such assays include peripheral blood mononuclear cells (PBMC) and Natural Killer (NK) cells. Alternatively, or additionally, ADCC activity of the le of interest may be assessed in vivo, e.g. in a animal model such as that disclosed in Clynes et al., Proc Natl Acad Sci USA 95, 652-656 (1998).

In some embodiments binding of the Fc domain to a complement component, specifically to C1q, is altered. Accordingly, in some embodiments wherein the Fc domain is engineered to have altered effector function, said altered effector function includes altered CDC. C1q binding assays may be carried out to determine whether the bispecific antigen binding molecule is able to bind C1q and hence has CDC ty. See e.g., C1q and C3c binding ELISA in and . To assess complement activation, a CDC assay may be performed (see, for example, Gazzano-Santoro et al., J Immunol Methods 202, 163 (1996); Cragg et al., Blood 101, 1045-1052 (2003); and Cragg and Glennie, Blood 103, 743 ). a) Decreased Fc receptor binding and/or effector function The Fc domain confers to the bispecific antigen binding molecule favorable pharmacokinetic properties, including a long serum half-life which butes to good accumulation in the target tissue and a favorable tissue-blood distribution ratio. At the same time it may, however, lead to undesirable targeting of the ific antigen binding molecule to cells expressing Fc receptors rather than to the preferred antigen-bearing cells. Moreover, the activation of Fc receptor signaling pathways may lead to cytokine release and severe side effects upon systemic administration.

Accordingly, in particular embodiments, the Fc domain of the bispecific antigen g molecule is engineered to have reduced binding affinity to an Fc or and/or reduced effector function, as compared to a non-engineered Fc domain. In one such embodiment the Fc domain (or the bispecific antigen g molecule sing said Fc domain) exhibits less than 50%, preferably less than 20%, more preferably less than 10% and most ably less than 5% of the binding affinity to an Fc receptor, as compared to a non-engineered Fc domain (or a bispecific antigen binding molecule comprising a non-engineered Fc ), and/or less than 50%, preferably less than 20%, more preferably less than 10% and most preferably less than 5% of the effector on, as compared to a gineered Fc domain domain (or a bispecific antigen binding molecule comprising a non-engineered Fc domain). In one embodiment, the Fc domain domain (or the bispecific antigen binding molecule comprising said Fc domain) does not substantially bind to an Fc receptor and/or induce effector function. In a particular embodiment the Fc receptor is an Fcγ receptor. In one embodiment the Fc receptor is a human Fc or. In one embodiment the Fc receptor is an activating Fc receptor. In a specific embodiment the Fc receptor is an activating human Fcγ receptor, more ically human FcγRIIIa, FcγRI or FcγRIIa, most specifically human FcγRIIIa. In one embodiment the effector function is one or more selected from the group of CDC, ADCC, ADCP, and cytokine secretion. In a particular embodiment the effector function is ADCC. In one embodiment the Fc domain domain exhibits substantially similar binding affinity to neonatal Fc receptor (FcRn), as compared to a nonengineered Fc domain. Substantially similar binding to FcRn is achieved when the Fc domain (or the bispecific antigen binding molecule comprising said Fc domain) exhibits greater than about 70%, particularly greater than about 80%, more particularly greater than about 90% of the binding affinity of a non-engineered Fc domain (or the bispecific antigen binding molecule comprising a non-engineered Fc domain) to FcRn.

In certain embodiments, the Fc domain of the bispecific n binding molecule comprises one or more amino acid mutation that reduces the binding affinity of the Fc domain to an Fc receptor and/or effector function. Typically, the same one or more amino acid mutation is present in each of the two subunits of the Fc domain. In one embodiment the amino acid mutation reduces the binding affinity of the Fc domain to an Fc receptor. In one embodiment the amino acid mutation reduces the binding affinity of the Fc domain to an Fc receptor by at least 2-fold, at least 5-fold, or at least 10-fold. In embodiments where there is more than one amino acid mutation that reduces the binding affinity of the Fc domain to the Fc or, the combination of these amino acid mutations may reduce the binding affinity of the Fc domain to an Fc receptor by at least 10- fold, at least 20-fold, or even at least 50-fold. In a ular embodiment the Fc receptor is an Fcγ receptor. In some embodiments the Fc receptor is a human Fc receptor. In some embodiments the Fc receptor is an activating Fc receptor. In a specific embodiment the Fc receptor is an activating human Fcγ receptor, more specifically human FcγRIIIa, FcγRI or FcγRIIa, most specifically human FcγRIIIa. ably, binding to each of these receptors is reduced. In some embodiments binding affinity to a complement component, specifically binding affinity to C1q, is also reduced. In one embodiment binding affinity to neonatal Fc receptor (FcRn) is not reduced.

In certain embodiments the Fc domain of the bispecific antigen g molecule is engineered to have d or function, as compared to a non-engineered Fc domain. The reduced effector function can include, but is not limited to, one or more of the ing: reduced complement dependent cytotoxicity (CDC), reduced antibody-dependent ediated cytotoxicity (ADCC), d antibody-dependent cellular phagocytosis (ADCP), reduced cytokine secretion, reduced immune complex-mediated antigen uptake by antigen-presenting cells, reduced g to NK cells, reduced g to macrophages, reduced binding to monocytes, reduced binding to rphonuclear cells, d direct signaling inducing apoptosis, reduced crosslinking of target-bound antibodies, reduced dendritic cell maturation, or reduced T cell priming. In one embodiment the d effector function is one or more selected from the group of reduced CDC, reduced ADCC, reduced ADCP, and d cytokine secretion. In a particular embodiment the reduced effector function is reduced ADCC. In one embodiment the reduced ADCC is less than 20% of the ADCC induced by a non-engineered Fc domain (or a bispecific antigen binding molecule comprising a non-engineered Fc domain).

In one ment the amino acid mutation that reduces the binding affinity of the Fc domain to an Fc receptor and/or effector function is an amino acid substitution. In one embodiment the Fc domain comprises an amino acid substitution at a on selected from the group of E233, L234, L235, N297, P331 and P329. In a more specific embodiment the Fc domain comprises an amino acid substitution at a position selected from the group of L234, L235 and P329. In some embodiments the Fc domain comprises the amino acid tutions L234A and L235A. In one such embodiment, the Fc domain is an IgG1 Fc , particularly a human IgG1 Fc domain. In one embodiment the Fc domain comprises an amino acid substitution at position P329. In a more specific embodiment the amino acid substitution is P329A or P329G, particularly P329G. In one embodiment the Fc domain comprises an amino acid substitution at position P329 and a further amino acid tution at a position selected from E233, L234, L235, N297 and P331. In a more specific embodiment the further amino acid tution is E233P, L234A, L235A, L235E, N297A, N297D or P331S. In ular embodiments the Fc domain comprises amino acid substitutions at positions P329, L234 and L235. In more particular embodiments the Fc domain comprises the amino acid mutations L234A, L235A and P329G (“P329G LALA”). In one such embodiment, the Fc domain is an IgG1 Fc , particularly a human IgG1 Fc domain. The “P329G LALA” combination of amino acid substitutions almost completely abolishes Fcγ receptor binding of a human IgG1 Fc domain, as described in PCT patent application no. , incorporated herein by reference in its entirety. also bes methods of preparing such mutant Fc domains and methods for ining its properties such as Fc receptor binding or effector functions.

IgG4 antibodies exhibit reduced binding affinity to Fc receptors and reduced effector functions as compared to IgG1 antibodies. Hence, in some embodiments the Fc domain of the bispecific antigen binding molecules described is an IgG4 Fc domain, particularly a human IgG4 Fc domain.

In one embodiment the IgG4 Fc domain ses amino acid tutions at position S228, specifically the amino acid substitution S228P. To further reduce its binding affinity to an Fc receptor and/or its effector function, in one embodiment the IgG4 Fc domain comprises an amino acid substitution at position L235, specifically the amino acid substitution L235E. In another embodiment, the IgG4 Fc domain comprises an amino acid substitution at position P329, specifically the amino acid substitution P329G. In a particular embodiment, the IgG4 Fc domain ses amino acid substitutions at positions S228, L235 and P329, specifically amino acid substitutions S228P, L235E and P329G. Such IgG4 Fc domain s and their Fcγ receptor binding properties are described in PCT patent application no. , incorporated herein by reference in its entirety.

In a particular ment the Fc domain exhibiting reduced binding affinity to an Fc receptor and/or reduced effector function, as compared to a native IgG1 Fc domain, is a human IgG1 Fc domain comprising the amino acid substitutions L234A, L235A and optionally P329G, or a human IgG4 Fc domain comprising the amino acid substitutions S228P, L235E and optionally P329G.

In certain ments N-glycosylation of the Fc domain has been eliminated. In one such ment the Fc domain comprises an amino acid mutation at position N297, particularly an amino acid substitution replacing asparagine by e (N297A) or aspartic acid (N297D).

In addition to the Fc domains described hereinabove and in PCT patent application no.

, Fc s with reduced Fc receptor binding and/or effector function also include those with substitution of one or more of Fc domain residues 238, 265, 269, 270, 297, 327 and 329 (U.S. Patent No. 056). Such Fc mutants include Fc mutants with substitutions at two or more of amino acid positions 265, 269, 270, 297 and 327, including the so-called “DANA” Fc mutant with substitution of residues 265 and 297 to alanine (US Patent No. 7,332,581).

Mutant Fc domains can be prepared by amino acid on, substitution, insertion or cation using genetic or chemical methods well known in the art. Genetic methods may include site-specific mutagenesis of the ng DNA sequence, PCR, gene synthesis, and the like. The correct nucleotide changes can be verified for e by sequencing. b) Increased Fc receptor g and/or effector function Conversely, there may be situations where it is desirable to in or even enhance Fc receptor binding and/or effector functions of the bispecific antigen binding molecules, for example when the bispecific antigen binding molecule is targeted to a highly specific tumor antigen. Hence, in certain embodiments the Fc domain of the bispecific antigen binding molecules described is engineered to have increased binding affinity to an Fc receptor. Increased binding affinity may be an increase in the binding affinity of the Fc domain to the Fc receptor by at least 2-fold, at least 5-fold, or at least 10-fold. In one embodiment the Fc receptor is an activating Fc receptor.

In a specific embodiment the Fc receptor is an Fcγ receptor, particularly a human Fcγ receptor.

In one ment the Fc receptor is selected from the group of FcγRIIIa, FcγRI and FcγRIIa.

In a particular embodiment the Fc receptor is FcγRIIIa.

In one such embodiment the Fc domain is engineered to have an altered oligosaccharide structure compared to a non-engineered Fc domain. In a particular such embodiment the Fc domain comprises an increased proportion of non-fucosylated oligosaccharides, compared to a non-engineered Fc domain. In a more specific ment, at least about 50%, more particularly at least about 70%, of the N-linked oligosaccharides in the Fc domain of the bispecific antigen binding molecule are non-fucosylated. The non-fucosylated oligosaccharides may be of the hybrid or complex type. In another specific embodiment the Fc domain ses an sed proportion of bisected oligosaccharides, compared to a non-engineered Fc domain. In a more ic embodiment, at least about 35%, particularly at least about 50%, more particularly at least about 70%, of the N-linked oligosaccharides in the Fc domain of the bispecific antigen binding molecule are bisected. The ed oligosaccharides may be of the hybrid or complex type. In yet another specific embodiment the Fc domain ses an increased proportion of bisected, non-fucosylated oligosaccharides, compared to a non-engineered Fc domain. In a more specific ment, at least about 15%, more particularly at least about 25%, at least about % or at least about 50%, of the N-linked oligosaccharides in the Fc domain of the bispecific n binding molecule are bisected, non-fucosylated. The bisected, non-fucosylated oligosaccharides may be of the hybrid or complex type.

The oligosaccharide structures in the bispecific antigen binding molecule Fc domain can be ed by methods well known in the art, e.g. by MALDI TOF mass spectrometry as bed in Umana et al., Nat Biotechnol 17, 176-180 (1999) or Ferrara et al., Biotechn Bioeng 93, 851- 861 (2006). The percentage of non-fucosylated oligosaccharides is the amount of oligosaccharides lacking fucose residues, relative to all oligosaccharides attached to Asn 297 (e.g. x, hybrid and high mannose structures) and identified in an N-glycosidase F d sample by MALDI TOF MS. Asn 297 refers to the asparagine residue located at about position 297 in the Fc domain (EU ing of Fc region residues); however, Asn297 may also be located about ± 3 amino acids upstream or downstream of position 297, i.e., between positions 294 and 300, due to minor sequence variations in globulins. The percentage of bisected, or bisected non-fucosylated, oligosaccharides is determined analogously.

Modification of the glycosylation in the Fc domain of the bispecific antigen binding molecule may result from production of the bispecific antigen binding molecule in a host cell that has been manipulated to express altered levels of one or more polypeptides having glycosyltransferase activity.

In one embodiment the Fc domain of the bispecific antigen binding le is engineered to have an altered oligosaccharide structure, as compared to a non-engineered Fc domain, by producing the bispecific antigen binding molecule in a host cell having altered activity of one or more yltransferase. Glycosyltransferases include for example β(1,4)-N- glucosaminyltransferase III (GnTIII), -galactosyltransferase (GalT), β(1,2)-N- acetylglucosaminyltransferase I (GnTI), β(1,2)-N-acetylglucosaminyltransferase II ) and α(1,6)-fucosyltransferase. In a specific embodiment the Fc domain of the bispecific antigen binding molecule is engineered to se an increased proportion of non-fucosylated oligosaccharides, as compared to a non-engineered Fc domain, by producing the bispecific n g molecule in a host cell having sed β(1,4)-N-acetylglucosaminyltransferase III I) activity. In an even more specific embodiment the host cell additionally has increased α-mannosidase II ) activity. The glycoengineering methodology that can be used for glycoengineering bispecific antigen binding les described has been described in greater detail in Umana et al., Nat Biotechnol 17, 176-180 (1999); Ferrara et al., Biotechn Bioeng 93, 851-861 (2006); WO 99/54342 (U.S. Pat. No. 6,602,684; EP 1071700); WO 2004/065540 (U.S. Pat. Appl. Publ. No. 2004/0241817; EP 1587921), WO 03/011878 (U.S. Pat.

Appl. Publ. No. 2003/0175884), the content of each of which is expressly incorporated herein by reference in its entirety.

Generally, any type of cultured cell line, including the cell lines discussed herein, can be used to generate cell lines for the production of bispecific antigen binding molecules with altered glycosylation pattern. Particular cell lines include CHO cells, BHK cells, NS0 cells, SP2/0 cells, YO myeloma cells, P3X63 mouse myeloma cells, PER cells, PER.C6 cells or hybridoma cells, and other mammalian cells. In certain embodiments, the host cells have been manipulated to express increased levels of one or more polypeptides having β(1,4)-N- acetylglucosaminyltransferase III (GnTIII) activity. In certain embodiments the host cells have been further manipulated to express sed levels of one or more polypeptides having αmannosidase II (ManII) activity. In a ic embodiment, the polypeptide having GnTIII ty is a fusion polypeptide comprising the catalytic domain of GnTIII and the Golgi localization domain of a heterologous Golgi resident polypeptide. Particularly, said Golgi localization domain is the Golgi localization domain of mannosidase II. Methods for generating such fusion polypeptides and using them to produce antibodies with increased effector functions are disclosed in Ferrara et al., Biotechn Bioeng 93, 851-861 (2006) and , the entire contents of which are expressly incorporated herein by nce.

The host cells which contain a coding sequence of a bispecific antigen binding molecule described and/or a coding ce of a polypeptide having glycosyltransferase activity, and which express the biologically active gene products, may be identified e.g. by DNA-DNA or DNA-RNA ization, the presence or absence of r" gene functions, assessing the level of transcription as measured by the expression of the respective mRNA transcripts in the host cell, or detection of the gene product as measured by immunoassay or by its biological activity - methods which are well known in the art. GnTIII or Man II activity can be detected e.g. by ing a lectin which binds to biosynthesis products of GnTIII or ManII, respectively. An example for such a lectin is the E4-PHA lectin which binds preferentially to oligosaccharides containing ing GlcNAc. Biosynthesis products (i.e. specific oligosaccharide structures) of polypeptides having GnTIII or ManII activity can also be detected by mass spectrometric analysis of oligosaccharides released from glycoproteins produced by cells expressing said polypeptides. Alternatively, a functional assay which measures the increased effector function and/or increased Fc receptor binding, mediated by bispecific antigen binding molecules produced by the cells engineered with the polypeptide having GnTIII or ManII activity may be used.

In r ment the Fc domain is ered to comprise an increased proportion of nonfucosylated oligosaccharides, as ed to a gineered Fc domain, by producing the bispecific antigen binding molecule in a host cell having sed α(1,6)-fucosyltransferase activity. A host cell having sed -fucosyltransferase activity may be a cell in which the α(1,6)-fucosyltransferase gene has been disrupted or ise deactivated, e.g. knocked out (see Yamane-Ohnuki et al., Biotech Bioeng 87, 614 (2004); Kanda et al., Biotechnol Bioeng 94(4), 680-688 (2006); Niwa et al., J Immunol Methods 306, 151-160 (2006)).

Other examples of cell lines e of producing defucosylated bispecific antigen binding molecules include Lec13 CHO cells deficient in protein fucosylation (Ripka et al., Arch Biochem Biophys 249, 533-545 (1986); US Pat. Appl. No. US 2003/0157108; and WO 2004/056312, especially at Example 11). The bispecific antigen binding molecules described can alternatively be glycoengineered to have reduced fucose residues in the Fc domain according to the techniques disclosed in EP 1 176 195 A1, WO 03/084570, WO 03/085119 and U.S. Pat.

Appl. Pub. Nos. 2003/0115614, 2004/093621, 2004/110282, 2004/110704, 2004/132140, US Pat. No. 6,946,292 (Kyowa), e.g. by reducing or abolishing the activity of a GDP-fucose transporter protein in the host cells used for bispecific n binding molecule tion.

Glycoengineered bispecific antigen binding molecules described may also be produced in expression systems that produce modified glycoproteins, such as those taught in WO 2003/056914 (GlycoFi, Inc.) or in and (Greenovation).

In one embodiment the Fc domain of the bispecific antigen binding molecule is ered to have increased or function, compared to a non-engineered Fc . The increased effector on can e, but is not limited to, one or more of the following: increased complement dependent cytotoxicity (CDC), increased antibody-dependent cell-mediated cytotoxicity (ADCC), increased antibody-dependent cellular phagocytosis (ADCP), increased cytokine secretion, increased immune x-mediated antigen uptake by antigen-presenting cells, increased binding to NK cells, increased binding to macrophages, increased binding to monocytes, increased binding to rphonuclear cells, increased direct ing ng apoptosis, increased crosslinking of target-bound dies, sed dendritic cell maturation, or increased T cell g.

In one embodiment the increased effector function is one or more selected from the group of increased CDC, increased ADCC, sed ADCP, and increased cytokine secretion. In a particular embodiment the increased effector function is increased ADCC. In one embodiment ADCC induced by an engineered Fc domain (or a bispecific antigen binding molecule comprising an engineered Fc domain) is a least 2-fold increased as compared to ADCC induced by a non-engineered Fc domain (or a bispecific antigen binding molecule comprising a non- ered Fc domain).

Antigens The bispecific antigen binding molecules described may bind to a variety of antigens. In certain embodiments the first and/or second antigen is an antigen associated with a pathological condition, such as an antigen presented on a tumor cell, on a virus-infected cell, or at a site of inflammation. Suitable antigens include cell surface antigens (for example, but not limited to, cell surface receptors), antigens free in blood serum, and/or antigens in the extracellular matrix.

In particular embodiments the antigen is a human antigen.

Non-limiting examples of antigens include tumor antigens such as MAGE, MART-1/Melan-A, gp100, Dipeptidyl peptidase IV (DPPIV), adenosine deaminase-binding protein (ADAbp), cyclophilin b, Colorectal associated n (CRC)-C017-1A/GA733, Carcinoembryonic Antigen (CEA) and its immunogenic epitopes CAP-1 and CAP-2, etv6, aml1, Prostate Specific Antigen (PSA) and its immunogenic epitopes PSA-1, PSA-2, and PSA-3, te-specific membrane antigen (PSMA), T-cell receptor/CD3-zeta chain, MAGE-family of tumor antigens (e.g., MAGE-A1, MAGE-A2, MAGE-A3, MAGE-A4, MAGE-A5, MAGE-A6, MAGE-A7, MAGE-A8, MAGE-A9, MAGE-A10, MAGE-A11, MAGE-A12, MAGE-Xp2 (MAGE-B2), MAGE-Xp3 (MAGE-B3), MAGE-Xp4 (MAGE-B4), MAGE-C1, MAGE-C2, MAGE-C3, MAGE-C4, MAGE-C5), GAGE-family of tumor antigens (e.g., GAGE-1, , GAGE-3, , GAGE-5, GAGE-6, , GAGE-8, GAGE-9), BAGE, RAGE, LAGE-1, NAG, GnT-V, MUM-1, CDK4, tyrosinase, p53, MUC family, HER2/neu, Her3, p21ras, RCAS1, α- fetoprotein, E-cadherin, α-catenin, β-catenin and γ-catenin, p120ctn, gp100 Pmel117, PRAME, NY-ESO-1, cdc27, adenomatous polyposis coli protein (APC), fodrin, Connexin 37, Ig-idiotype, p15, gp75, GM2 and GD2 osides, Smad family of tumor antigens, lmp-1, P1A, EBV- encoded r n (EBNA)-1, brain glycogen phosphorylase, SSX-1, SSX-2 (HOM-MEL- 40), SSX-1, SSX-4, SSX-5, SCP-1 and CT-7, and c-erbB-2; ECM ns such as syndecan, heparanase, integrins, osteopontin, link, cadherins, laminin, laminin type EGF, lectin, fibronectin and its alternatively spliced domains (e.g. the Extra Domain B), notch, various forms of tenascin (e.g. tenascin C) and its atively spliced domains (e.g. the A1 or A2 domain of tenascin-C), and matrixin; Fibroblast Activation Protein (FAP), Epidermal Growth Factor Receptor (EGFR), CD2 (T-cell surface antigen), CD3 (heteromultimer associated with the TCR), CD19, CD22 (B- cell receptor), CD23 (low affinity IgE receptor), CD25 (IL-2 receptor α chain), CD30 (cytokine receptor), CD33 (myeloid cell e antigen), CD40 (tumor necrosis factor or), IL-6R (IL6 receptor), CD20, Melanoma-associated Chondroitin Sulfate Proteoglycan (MCSP), nlike growth factor-1 receptor (IGF-1R), and PDGFβR (β platelet-derived growth factor receptor).

In a ic ment the first and second antigen are selected from the group of Fibroblast tion Protein (FAP), ma-associated Chondroitin Sulfate Proteoglycan (MCSP), Epidermal Growth Factor Receptor (EGFR), Her3, c-Met, Carcinoembryonic Antigen (CEA), CD33 and CD3.

In a particular embodiment the first n is CD3, particularly human or cynomolgus CD3, most particularly human CD3. In some embodiments, the first n is the epsilon subunit of CD3. In one embodiment, the first Fab fragment can compete with monoclonal antibody H2C (described in PCT publication no. WO2008/119567) for binding an epitope of CD3. In a particular embodiment, the first Fab fragment can compete with monoclonal antibody SP34 (described in Pessano et al., EMBO J 4, 337-340 (1985)) for binding an epitope of CD3. In another embodiment, the first Fab fragment can compete with monoclonal antibody V9 (described in Rodrigues et al., Int J Cancer Suppl 7, 45-50 (1992) and US patent no. 6,054,297) for binding an epitope of CD3. In yet another ment, the first Fab fragment can compete with onal antibody FN18 (described in Nooij et al., Eur J Immunol 19, 981-984 (1986)) for binding an epitope of CD3. In one embodiment, the first Fab fragment is specific for CD3 and comprises the heavy chain CDR1 of SEQ ID NO: 77, the heavy chain CDR2 of SEQ ID NO: 78, the heavy chain CDR3 of SEQ ID NO: 79, the light chain CDR1 of SEQ ID NO: 81, the light chain CDR2 of SEQ ID NO: 82, and the light chain CDR3 of SEQ ID NO: 83. In a further embodiment, the Fab fragment that is specific for CD3 comprises a heavy chain variable region sequence that is at least about 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% or 100% identical to SEQ ID NO: 80 and a light chain variable region sequence that is at least about 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% or 100% identical to SEQ ID NO: 84, or variants thereof that retain functionality. In r embodiment, the first Fab fragment is specific for CD3 and comprises the heavy chain CDR1 of SEQ ID NO: 104, the heavy chain CDR2 of SEQ ID NO: 105, the heavy chain CDR3 of SEQ ID NO: 106, the light chain CDR1 of SEQ ID NO: 108, the light chain CDR2 of SEQ ID NO: 109, and the light chain CDR3 of SEQ ID NO: 110. In a r ment, the Fab fragment that is specific for CD3 comprises a heavy chain variable region ce that is at least about 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% or 100% identical to SEQ ID NO: 107 and a light chain variable region ce that is at least about 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% or 100% identical to SEQ ID NO: 111, or variants thereof that retain functionality.

In a particular ment described, the bispecific antigen binding le is capable of simultaneous binding to a target cell antigen, particularly a tumor cell antigen, and CD3. In one embodiment, the bispecific antigen binding molecule is capable of crosslinking a T cell and a target cell by simultaneous binding to a target cell antigen and CD3. In an even more particular embodiment, such simultaneous binding results in lysis of the target cell, particularly a tumor cell. In one embodiment, such simultaneous binding results in activation of the T cell. In other embodiments, such aneous binding s in a cellular response of a T lymphocyte, particularly a cytotoxic T cyte, selected from the group of: eration, differentiation, cytokine secretion, cytotoxic effector molecule release, xic ty, and sion of activation markers. In one embodiment, binding of the bispecific antigen binding molecule to CD3 without simultaneous binding to the target cell antigen does not result in T cell activation.

In one embodiment, the bispecific antigen binding molecule is capable of re-directing xic activity of a T cell to a target cell. In a particular embodiment, said re-direction is independent of MHC-mediated peptide antigen presentation by the target cell and and/or specificity of the T cell.

Particularly, a T cell according to any of the embodiments described is a cytotoxic T cell. In some embodiments the T cell is a CD4+ or a CD8+ T cell, particularly a CD8+ T cell.

In one embodiment, the first antigen is c-Met, particularly human c-Met. In one embodiment, the first Fab fragment can compete with monoclonal antibody 5D5 (described e.g. in US patent no. 7,476,724, which is incorporated herein by reference in its entirety) for binding an epitope of c- Met. In one embodiment, the first Fab fragment is specific for c-Met and comprises the heavy chain CDR1 of SEQ ID NO: 63, the heavy chain CDR2 of SEQ ID NO: 64, the heavy chain CDR3 of SEQ ID NO: 65, the light chain CDR1 of SEQ ID NO: 67, the light chain CDR2 of SEQ ID NO: 68, and the light chain CDR3 of SEQ ID NO: 69. In a further embodiment, the Fab fragment that is specific for c-Met comprises a heavy chain variable region sequence that is at least about 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% or 100% identical to SEQ ID NO: 66 and a light chain variable region sequence that is at least about 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% or 100% cal to SEQ ID NO: 70, or variants thereof that retain functionality.

In particular ments the second antigen is a tumor-associated antigen, specficially an antigen presented on a tumor cell or a cell of the tumor stroma. In one such embodiment the second antigen is selected from the group of Fibroblast tion Protein (FAP), Melanomaassociated Chondroitin Sulfate Proteoglycan , Epidermal Growth Factor Receptor (EGFR), Her3, CD33, and Carcinoembryonic Antigen (CEA).

In one embodiment the second antigen is Melanoma-associated Chondroitin e Proteoglycan (MCSP). In another embodiment second and optionally the third Fab fragment can compete with monoclonal dy LC007 (see SEQ ID NOs 18 and 22) for binding to an epitope of MCSP. In one embodiment, the Fab fragment that is specific for MCSP comprises the heavy chain CDR1 of SEQ ID NO: 15, the heavy chain CDR2 of SEQ ID NO: 16, the heavy chain CDR3 of SEQ ID NO: 17, the light chain CDR1 of SEQ ID NO: 19, the light chain CDR2 of SEQ ID NO: 20, and the light chain CDR3 of SEQ ID NO: 21. In a further embodiment, the Fab fragment that is specific for MCSP comprises a heavy chain variable region ce that is at least about 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% or 100% cal to SEQ ID NO: 18 and a light chain variable region ce that is at least about 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% or 100% identical to SEQ ID NO: 22, or ts f that retain functionality. In another ment second and optionally the third Fab fragment can compete with onal antibody M4-3 ML2 (see SEQ ID NOs 99 and 103) for binding to an epitope of MCSP. In one embodiment, the Fab fragment that is specific for MCSP comprises the heavy chain CDR1 of SEQ ID NO: 96, the heavy chain CDR2 of SEQ ID NO: 97, the heavy chain CDR3 of SEQ ID NO: 98, the light chain CDR1 of SEQ ID NO: 100, the light chain CDR2 of SEQ ID NO: 101, and the light chain CDR3 of SEQ ID NO: 102. In a further embodiment, the Fab fragment that is specific for MCSP comprises a heavy chain variable region sequence that is at least about 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% or 100% identical to SEQ ID NO: 99 and a light chain variable region sequence that is at least about 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% or 100% identical to SEQ ID NO: 103, or variants thereof that retain functionality.

In yet another embodiment the bispecific antigen binding molecule comprises the polypeptide sequence of SEQ ID NO: 1, the polypeptide sequence of SEQ ID NO: 2, the polypeptide sequence of SEQ ID NO: 3 and the polypeptide sequence of SEQ ID NO: 4, or variants thereof that retain functionality. In a further embodiment the bispecific n binding molecule comprises the polypeptide sequence of SEQ ID NO: 1, the polypeptide sequence of SEQ ID NO: 3, the polypeptide sequence of SEQ ID NO: 4 and the polypeptide sequence of SEQ ID NO: 5, or variants thereof that retain functionality. In yet another embodiment the bispecific antigen binding le comprises the polypeptide sequence of SEQ ID NO: 4, the polypeptide sequence of SEQ ID NO: 5, the polypeptide sequence of SEQ ID NO: 6 and the polypeptide sequence of SEQ ID NO: 7, or ts thereof that retain functionality. In still another embodiment the bispecific antigen binding molecule comprises the polypeptide ce of SEQ ID NO: 4, the polypeptide sequence of SEQ ID NO: 5, the ptide sequence of SEQ ID NO: 1 and the polypeptide sequence of SEQ ID NO: 85, or variants thereof that retain functionality.

In a further embodiment the bispecific antigen binding molecule comprises the polypeptide sequence of SEQ ID NO: 1, the ptide sequence of SEQ ID NO: 3, the polypeptide sequence of SEQ ID NO: 4 and the polypeptide sequence of SEQ ID NO: 86, or variants thereof that retain functionality. In still a further embodiment the bispecific antigen binding molecule comprises the ptide sequence of SEQ ID NO: 4, the polypeptide sequence of SEQ ID NO: 87, the polypeptide sequence of SEQ ID NO: 89 and the ptide ce of SEQ ID NO: 90, or variants thereof that retain functionality. In a r embodiment the ific antigen binding molecule comprises the polypeptide sequence of SEQ ID NO: 3, the polypeptide sequence of SEQ ID NO: 91, the polypeptide sequence of SEQ ID NO: 92 and the polypeptide sequence of SEQ ID NO: 93, or variants thereof that retain functionality. In still another ment the bispecific antigen g molecule ses the polypeptide sequence of SEQ ID NO: 87, the polypeptide sequence of SEQ ID NO: 91, the polypeptide sequence of SEQ ID NO: 93 and the polypeptide sequence of SEQ ID NO: 94, or variants thereof that retain functionality.

In one embodiment the second antigen is Carcinoembryonic Antigen (CEA). In another embodiment the second and optionally the third Fab fragment can compete with monoclonal antibody CH1A1A for binding to an epitope of CEA. See PCT publication , incorporated herein by reference in its entirety. In one embodiment, the Fab fragment that is specific for CEA comprises the heavy chain CDR1 of SEQ ID NO: 39, the heavy chain CDR2 of SEQ ID NO: 40, the heavy chain CDR3 of SEQ ID NO: 41, the light chain CDR1 of SEQ ID NO: 43, the light chain CDR2 of SEQ ID NO: 44, and the light chain CDR3 of SEQ ID NO: 45.

In a further embodiment, the Fab fragment that is specific for CEA comprises a heavy chain variable region sequence that is at least about 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% or 100% identical to SEQ ID NO: 42 and a light chain variable region sequence that is at least about 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% or 100% identical to SEQ ID NO: 46, or variants thereof that retain onality.

In yet another embodiment the bispecific antigen binding molecule comprises the polypeptide sequence of SEQ ID NO: 3, the polypeptide sequence of SEQ ID NO: 8, the polypeptide sequence of SEQ ID NO: 9 and the polypeptide sequence of SEQ ID NO: 10, or variants thereof that retain functionality. In still another embodiment the bispecific antigen binding molecule ses the polypeptide ce of SEQ ID NO: 9, the polypeptide sequence of SEQ ID NO: , the polypeptide sequence of SEQ ID NO: 87 and the polypeptide sequence of SEQ ID NO: 95, or variants thereof that retain functionality.

In one embodiment the second antigen is Her3. In another embodiment the second and optionally the third Fab fragment can compete with monoclonal antibody Mab 205.10 for g to an epitope of Her3. See PCT publication no. , incorporated herein by reference in its ty. In one embodiment, the Fab nt that is specific for Her3 comprises the heavy chain CDR1 of SEQ ID NO: 55, the heavy chain CDR2 of SEQ ID NO: 56, the heavy chain CDR3 of SEQ ID NO: 57, the light chain CDR1 of SEQ ID NO: 59, the light chain CDR2 of SEQ ID NO: 60, and the light chain CDR3 of SEQ ID NO: 61. In a further embodiment, the Fab fragment that is specific for Her3 comprises a heavy chain variable region sequence that is at least about 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% or 100% identical to SEQ ID NO: 58 and a light chain variable region sequence that is at least about 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% or 100% identical to SEQ ID NO: 62, or variants thereof that retain functionality.

In yet another embodiment the bispecific n binding molecule comprises the polypeptide sequence of SEQ ID NO: 11, the polypeptide sequence of SEQ ID NO: 12, the polypeptide ce of SEQ ID NO: 13 and the ptide sequence of SEQ ID NO: 14, or variants thereof that retain functionality.

In one embodiment the second n is epidermal growth factor receptor (EGFR). In another embodiment the second and optionally the third Fab fragment can compete with monoclonal antibody GA201 for binding to an epitope of EGFR. See PCT publication , incorporated herein by reference in its entirety. In one embodiment, the Fab fragment that is specific for EGFR comprises the heavy chain CDR1 of SEQ ID NO: 23, the heavy chain CDR2 of SEQ ID NO: 24, the heavy chain CDR3 of SEQ ID NO: 25, the light chain CDR1 of SEQ ID NO: 27, the light chain CDR2 of SEQ ID NO: 28, and the light chain CDR3 of SEQ ID NO: 29.

In a further embodiment, the Fab nt that is specific for EGFR comprises a heavy chain variable region sequence that is at least about 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% or 100% identical to SEQ ID NO: 26 and a light chain variable region sequence that is at least about 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% or 100% identical to SEQ ID NO: 30, or ts thereof that retain functionality.

In one embodiment the second antigen is fibroblast activation protein (FAP). In another embodiment the second and optionally the third Fab nt can compete with monoclonal antibody 3F2 for binding to an epitope of FAP. See PCT publication , incorporated herein by reference in its entirety. In one ment, the Fab fragment that is specific for FAP comprises the heavy chain CDR1 of SEQ ID NO: 31, the heavy chain CDR2 of SEQ ID NO: 32, the heavy chain CDR3 of SEQ ID NO: 33, the light chain CDR1 of SEQ ID NO: 35, the light chain CDR2 of SEQ ID NO: 36, and the light chain CDR3 of SEQ ID NO: 37.

In a further embodiment, the Fab fragment that is specific for FAP comprises a heavy chain variable region sequence that is at least about 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% or 100% cal to SEQ ID NO: 34 and a light chain variable region sequence that is at least about 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% or 100% identical to SEQ ID NO: 38, or variants thereof that retain functionality.

In one embodiment the second antigen is CD33. In one embodiment, the Fab fragment that is specific for CD33 comprises the heavy chain CDR1 of SEQ ID NO: 47, the heavy chain CDR2 of SEQ ID NO: 48, the heavy chain CDR3 of SEQ ID NO: 49, the light chain CDR1 of SEQ ID NO: 51, the light chain CDR2 of SEQ ID NO: 52, and the light chain CDR3 of SEQ ID NO: 53.

In a further embodiment, the Fab fragment that is specific for CD33 comprises a heavy chain variable region sequence that is at least about 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% or 100% identical to SEQ ID NO: 50 and a light chain variable region sequence that is at least about 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% or 100% cal to SEQ ID NO: 54, or variants thereof that retain functionality.

Polynucleotides Also described are isolated polynucleotides encoding a bispecific antigen g molecule as described herein or a fragment thereof. The polynucleotides ng bispecific antigen g molecules described may be expressed as a single polynucleotide that encodes the entire bispecific antigen binding molecule or as le (e.g., two or more) cleotides that are co-expressed. Polypeptides encoded by polynucleotides that are co-expressed may associate through, e.g., ide bonds or other means to form a functional bispecific antigen binding molecule. For e, the light chain portion of a Fab fragment may be encoded by a separate polynucleotide from the portion of the ific antigen binding molecule comprising the heavy chain portion of the Fab fragment, an Fc domain subunit and optionally (part of) another Fab fragment. When co-expressed, the heavy chain polypeptides will associate with the light chain polypeptides to form the Fab fragment. In another example, the portion of the bispecific antigen binding molecule comprising one of the two Fc domain subunits and optionally (part of) one or more Fab fragments could be encoded by a separate polynucleotide from the portion of the bispecific antigen binding molecule comprising the the other of the two Fc domain subunits and optionally (part of) a Fab fragment. When ressed, the Fc domain subunits will associate to form the Fc domain.

In one embodiment, an isolated polynucleotide described encodes the first Fc domain subunit, the heavy chain of the second Fab fragment and the heavy chain of the first Fab fragment. In a more specific embodiment, the isolated polynucleotide encodes a polypeptide wherein a VL region shares a carboxy-terminal peptide bond with a CH1 region, which in turn shares a carboxy-terminal peptide bond with a e linker, which in turn shares a carboxy-terminal peptide bond with an immunoglobulin heavy chain (VH-CH1-HR-CH2-CH3-(CH4)). In another specific embodiment the ed polynucleotide encodes a polypeptide wherein a Fab heavy chain (VH-CH1) shares a carboxy-terminal peptide bond with a peptide linker, which in turn shares a carboxy-terminal peptide bond with a VL region, which in turn shares a carboxyterminal peptide bond with a CH1 region, which in turn shares a carboxy-terminal peptide bond with an Fc domain subunit ing an immunoglobulin hinge region (HR-CH2-CH3-(CH4)).

In yet another specific embodiment, the isolated polynucleotide encodes a polypeptide wherein a VH region shares a carboxy-terminal peptide bond with a CL region, which in turn shares a y-terminal peptide bond with a peptide linker, which in turn shares a carboxy-terminal peptide bond with an immunoglobulin heavy chain (VH-CH1-HR-CH2-CH3-(CH4)). In still r specific embodiment the isolated polynucleotide encodes a polypeptide wherein a Fab heavy chain (VH-CH1) shares a y-terminal peptide bond with a peptide linker, which in turn shares a y-terminal peptide bond with a VH region, which in turn shares a carboxyterminal peptide bond with a CL region, which in turn shares a carboxy-terminal peptide bond with an Fc domain subunit ing an globulin hinge region (HR-CH2-CH3-(CH4)).

In yet another specific embodiment, the ed polynucleotide encodes a ptide wherein an immunoglobulin heavy chain ((VH-CH1-HR-CH2-CH3-(CH4)) shares a carboxy-terminal peptide bond with a peptide linker, which in turn shares a y-terminal peptide bond with a VH , which in turn shares a carboxy-terminal peptide bond with a CL region.

In further embodiments, an isolated polynucleotide described encodes the second Fc domain subunit and optionally the heavy chain of a third Fab fragment. In a specific embodiment, the isolated cleotide encodes an immunoglobulin heavy chain ((VH-CH1-HR-CH2-CH3- (CH4)). In another specific embodiment, the isolated polynucleotide encodes an Fc domain subunit, optionally including an antibody hinge region ((HR)-CH2-CH3-(CH4)).

In still further ments, an isolated polynucleotide described encodes one or more light chain comprised in the bispecific antigen binding molecule. In a ic embodiment, the isolated polynucleotide encodes an immunoglobulin light chain (VL-CL). In another specific embodiment, the ed polynucleotide encodes a polypeptide wherein a VL region shares a carboxy-terminal peptide bond with a CH1 region. In yet another specific embodiment, the isolated polynucleotide encodes a polypeptide wherein a VH region shares a carboxy-terminal peptide bond with a CL region. In still another specific embodiment, the isolated polynucleotide encodes a polypeptide wherein a VH region shares a carboxy-terminal peptide bond with a CL region, which in turn shares a carboxy-terminal peptide bond with a Fab light chain (VL-CL). In yet another specific embodiment, the ed polynucleotide encodes a polypeptide wherein a Fab light chain (VL-CL) shares a carboxy-terminal peptide bond with a VH , which in turn shares a y-terminal peptide bond with a CL region.

In another embodiment, described is an isolated polynucleotide encoding a bispecific antigen binding molecule described or a nt thereof, wherein the polynucleotide comprises a sequence that encodes a le region sequence as shown in SEQ ID NOs 18, 22, 26, 30, 34, 38, 42, 46, 50, 54, 58, 62, 66, 70, 80, 84, 99, 103, 107 and 111. In another embodiment, described is an isolated polynucleotide encoding a bispecific n g molecule or nt thereof, wherein the polynucleotide comprises a sequence that encodes a polypeptide sequence as shown in SEQ ID NOs 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94 and 95. In another embodiment, described is an isolated polynucleotide encoding a bispecific antigen binding molecule described or a fragment thereof, wherein the polynucleotide comprises a sequence that encodes a variable region sequence that is at least about 80%, 85%, 90%, 95%, 96%, 97%, 98%, or 99% identical to an amino acid sequence in SEQ ID NOs 18, 22, 26, 30, 34, 38, 42, 46, 50, 54, 58, 62, 66, 70, 80, 84, 99, 103, 107 or 111. In r embodiment, described is an isolated polynucleotide encoding a bispecific antigen binding le or nt thereof, wherein the polynucleotide comprises a sequence that encodes a polypeptide sequence that is at least 80%, 85%, 90%, 95%, 96%, 97%, 98%, or 99% identical to an amino acid sequence in SEQ ID NOs 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94 or 95. Also described is an isolated polynucleotide ng a bispecific antigen binding molecule described or a fragment thereof, wherein the polynucleotide comprises a sequence that encodes the variable region ce of SEQ ID NOs 18, 22, 26, 30, 34, 38, 42, 46, 50, 54, 58, 62, 66, 70, 80, 84, 99, 103, 107 or 111 with conservative amino acid substitutions. Also described is an isolated polynucleotide encoding a bispecific antigen binding molecule or fragment thereof, n the polynucleotide comprises a sequence that encodes the polypeptide sequence of SEQ ID NOs 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94 or 95 with conservative amino acid substitutions.

In n embodiments the polynucleotide or c acid is DNA. In other embodiments, a polynucleotide described is RNA, for e, in the form of messenger RNA . RNA described may be single stranded or double stranded.

Recombinant Methods Bispecific antigen binding les described may be obtained, for example, by solid-state peptide synthesis (e.g. Merrifield solid phase sis) or recombinant production. For recombinant production one or more polynucleotide encoding the bispecific antigen binding molecule (fragment), e.g., as described above, is isolated and inserted into one or more vectors for r cloning and/or expression in a host cell. Such polynucleotide may be readily isolated and sequenced using conventional procedures. In one embodiment a vector, preferably an expression vector, comprises one or more of the polynucleotides described.. s which are well known to those skilled in the art can be used to construct expression s containing the coding sequence of a bispecific antigen binding molecule (fragment) along with appropriate transcriptional/translational control signals. These methods include in vitro recombinant DNA ques, synthetic techniques and in vivo recombination/genetic recombination. See, for example, the techniques described in is et al., MOLECULAR CLONING: A LABORATORY , Cold Spring Harbor Laboratory, N.Y. (1989); and l et al., CURRENT PROTOCOLS IN MOLECULAR BIOLOGY, Greene Publishing Associates and Wiley Interscience, N.Y (1989). The expression vector can be part of a plasmid, virus, or may be a nucleic acid fragment. The expression vector includes an expression cassette into which the polynucleotide encoding the bispecific antigen binding molecule (fragment) (i.e. the coding region) is cloned in operable association with a promoter and/or other transcription or translation control elements.

As used , a "coding region" is a portion of nucleic acid which consists of codons translated into amino acids. Although a "stop codon" (TAG, TGA, or TAA) is not translated into an amino acid, it may be considered to be part of a coding region, if present, but any flanking sequences, for example promoters, ribosome binding sites, transcriptional terminators, s, 5' and 3' untranslated regions, and the like, are not part of a coding region. Two or more coding regions can be present in a single polynucleotide construct, e.g. on a single vector, or in separate polynucleotide constructs, e.g. on separate (different) vectors. Furthermore, any vector may contain a single coding region, or may comprise two or more coding regions, e.g. a vector described may encode one or more polypeptides, which are post- or nslationally separated into the final proteins via proteolytic cleavage. In addition, a vector, polynucleotide, or c acid described may encode heterologous coding s, either fused or unfused to a polynucleotide encoding the bispecific antigen binding molecule (fragment) described, or t or derivative thereof. Heterologous coding regions include without limitation specialized elements or , such as a secretory signal e or a heterologous functional domain. An operable association is when a coding region for a gene product, e.g. a polypeptide, is associated with one or more regulatory sequences in such a way as to place expression of the gene product under the influence or control of the regulatory sequence(s). Two DNA fragments (such as a polypeptide coding region and a promoter associated therewith) are "operably associated" if induction of promoter function results in the transcription of mRNA encoding the desired gene t and if the nature of the linkage between the two DNA fragments does not interfere with the ability of the expression regulatory sequences to direct the expression of the gene product or interfere with the ability of the DNA template to be ribed. Thus, a promoter region would be operably associated with a nucleic acid encoding a polypeptide if the promoter was capable of effecting transcription of that nucleic acid. The promoter may be a cell-specific promoter that directs ntial transcription of the DNA only in predetermined cells. Other transcription control elements, besides a promoter, for example enhancers, operators, repressors, and ription termination signals, can be operably associated with the cleotide to direct cell-specific transcription. le promoters and other transcription control regions are disclosed herein. A variety of transcription control regions are known to those skilled in the art.

These include, without tion, transcription control regions, which function in vertebrate cells, such as, but not limited to, promoter and enhancer segments from cytomegaloviruses (e.g. the immediate early promoter, in ction with intron-A), simian virus 40 (e.g. the early promoter), and retroviruses (such as, e.g. Rous a virus). Other transcription control regions e those derived from rate genes such as actin, heat shock protein, bovine growth hormone and rabbit â-globin, as well as other sequences e of lling gene expression in eukaryotic cells. Additional suitable transcription control regions include tissuespecific promoters and enhancers as well as inducible promoters (e.g. promoters inducible tetracyclins). Similarly, a variety of translation control elements are known to those of ry skill in the art. These include, but are not limited to ribosome binding sites, ation initiation and termination codons, and elements d from viral systems (particularly an internal ribosome entry site, or IRES, also referred to as a CITE sequence). The sion cassette may also include other features such as an origin of replication, and/or chromosome ation elements such as retroviral long terminal repeats (LTRs), or adeno-associated viral (AAV) inverted terminal repeats (ITRs).

Polynucleotide and nucleic acid coding regions described may be associated with additional coding regions which encode secretory or signal peptides, which direct the secretion of a polypeptide encoded by a polynucleotide described. For example, if ion of the bispecific antigen binding molecule is desired, DNA encoding a signal sequence may be placed am of the nucleic acid encoding a bispecific antigen binding molecule described or a fragment thereof. According to the signal hypothesis, proteins secreted by mammalian cells have a signal e or secretory leader sequence which is cleaved from the mature protein once export of the growing protein chain across the rough endoplasmic reticulum has been initiated. Those of ordinary skill in the art are aware that polypeptides ed by vertebrate cells generally have a signal peptide fused to the N-terminus of the polypeptide, which is cleaved from the translated polypeptide to produce a ed or "mature" form of the polypeptide. In certain embodiments, the native signal peptide, e.g. an immunoglobulin heavy chain or light chain signal peptide is used, or a functional derivative of that sequence that retains the ability to direct the secretion of the polypeptide that is operably associated with it. Alternatively, a heterologous mammalian signal e, or a functional derivative thereof, may be used. For example, the ype leader sequence may be substituted with the leader sequence of human tissue plasminogen activator (TPA) or mouse β-glucuronidase. Exemplary amino acid sequences of secretory signal peptides are given in SEQ ID NOs 74-76.

DNA ng a short protein sequence that could be used to facilitate later purification (e.g. a histidine tag) or assist in labeling the bispecific antigen binding molecule may be included within or at the ends of the bispecific antigen binding molecule (fragment) encoding polynucleotide.

Also described is a host cell comprising one or more polynucleotides bed .. In certain embodiments described is a host cell comprising one or more vectors described.. The cleotides and vectors may incorporate any of the features, singly or in combination, described herein in relation to polynucleotides and vectors, respectively. In one such embodiment a host cell comprises (e.g. has been transformed or transfected with) a vector comprising a polynucleotide that s (part of) a bispecific antigen binding molecule described. As used herein, the term "host cell" refers to any kind of cellular system which can be engineered to generate the bispecific antigen binding molecules bed or fragments thereof.

Host cells suitable for replicating and for supporting expression of bispecific antigen binding les are well known in the art. Such cells may be transfected or transduced as appropriate with the particular expression vector and large quantities of vector ning cells can be grown for seeding large scale fermenters to obtain sufficient quantities of the bispecific antigen g molecule for clinical applications. Suitable host cells include prokaryotic microorganisms, such as E. coli, or various eukaryotic cells, such as Chinese hamster ovary cells (CHO), insect cells, or the like. For example, polypeptides may be produced in bacteria in particular when glycosylation is not . After expression, the polypeptide may be isolated from the bacterial cell paste in a soluble fraction and can be further purified. In addition to yotes, otic microbes such as filamentous fungi or yeast are suitable cloning or expression hosts for polypeptide-encoding vectors, including fungi and yeast strains whose glycosylation pathways have been “humanized”, resulting in the production of a polypeptide with a partially or fully human glycosylation pattern.

See Gerngross, Nat Biotech 22, 414 (2004), and Li et al., Nat Biotech 24, 210-215 (2006).

Suitable host cells for the expression of (glycosylated) polypeptides are also derived from multicellular organisms (invertebrates and vertebrates). Examples of invertebrate cells include plant and insect cells. Numerous baculoviral strains have been identified which may be used in conjunction with insect cells, particularly for transfection of Spodoptera frugiperda cells. Plant cell cultures can also be utilized as hosts. See e.g. US Patent Nos. 5,959,177, 6,040,498, 6,420,548, 7,125,978, and 6,417,429 (describing PLANTIBODIESTM technology for producing antibodies in transgenic plants). Vertebrate cells may also be used as hosts. For example, ian cell lines that are adapted to grow in sion may be useful. Other examples of useful mammalian host cell lines are monkey kidney CV1 line transformed by SV40 (COS-7); human embryonic kidney line (293 or 293T cells as described, e.g., in Graham et al., J Gen Virol 36, 59 (1977)), baby r kidney cells (BHK), mouse sertoli cells (TM4 cells as described, e.g., in Mather, Biol Reprod 23, 1 (1980)), monkey kidney cells (CV1), African green monkey kidney cells (VERO-76), human al carcinoma cells (HELA), canine kidney cells (MDCK), buffalo rat liver cells (BRL 3A), human lung cells (W138), human liver cells (Hep G2), mouse mammary tumor cells (MMT 060562), TRI cells (as described, e.g., in Mather et al., Annals N.Y. Acad Sci 383, 44-68 (1982)), MRC 5 cells, and FS4 cells. Other useful mammalian host cell lines include Chinese hamster ovary (CHO) cells, including dhfr- CHO cells (Urlaub et al., Proc Natl Acad Sci USA 77, 4216 (1980)); and a cell lines such as YO, NS0, P3X63 and Sp2/0. For a review of certain mammalian host cell lines suitable for protein tion, see, e.g., Yazaki and Wu, Methods in Molecular Biology, Vol. 248 . Lo, ed., Humana Press, Totowa, NJ), pp. 255-268 (2003). Host cells e cultured cells, e.g., mammalian cultured cells, yeast cells, insect cells, bacterial cells and plant cells, to name only a few, but also cells comprised within a transgenic , transgenic plant or cultured plant or animal tissue. In one embodiment, the host cell is a eukaryotic cell, preferably a mammalian cell, such as a e Hamster Ovary (CHO) cell, a human embryonic kidney (HEK) cell or a lymphoid cell (e.g., Y0, NS0, Sp20 cell).

Standard technologies are known in the art to express foreign genes in these systems. Cells expressing a polypeptide comprising either the heavy or the light chain of an antigen binding domain such as an antibody, may be engineered so as to also express the other of the antibody chains such that the expressed product is an antibody that has both a heavy and a light chain.

In one embodiment, described is a method of producing a ific antigen binding molecule bed, wherein the method comprises culturing a host cell comprising a polynucleotide encoding the bispecific antigen binding molecule, as bed herein, under conditions le for expression of the bispecific antigen binding le, and recovering the bispecific antigen binding molecule from the host cell (or host cell culture medium).

The components of the bispecific antigen binding molecule are genetically fused to each other.

Bispecific antigen binding molecule can be designed such that its components are fused directly to each other or indirectly through a linker sequence. The composition and length of the linker may be determined in accordance with s well known in the art and may be tested for efficacy. Examples of linker ces between different components of bispecific antigen binding molecules are found in the sequences described herein. Additional sequences may also be included to incorporate a ge site to te the individual components of the fusion if desired, for example an endopeptidase recognition sequence.

In certain embodiments the Fab fragments forming part of the bispecific antigen binding molecules comprise at least an antibody variable region capable of g an antigenic determinant. Variable regions can form part of and be derived from naturally or non-naturally occurring antibodies and fragments thereof. Methods to produce polyclonal antibodies and monoclonal antibodies are well known in the art (see e.g. Harlow and Lane, "Antibodies, a laboratory manual", Cold Spring Harbor tory, 1988). Non-naturally occurring antibodies can be ucted using solid phase-peptide synthesis, can be produced recombinantly (e.g. as described in U.S. patent No. 4,186,567) or can be obtained, for example, by screening combinatorial ies comprising variable heavy chains and variable light chains (see e.g. U.S.

Patent. No. 5,969,108 to McCafferty).

Any animal species of dy, antibody nt, antigen binding domain or variable region can be used in the bispecific antigen binding molecules described. Non-limiting antibodies, antibody fragments, antigen binding domains or variable regions useful herein can be of murine, primate, or human origin. If the bispecific antigen binding molecule is intended for human use, a chimeric form of antibody may be used wherein the constant regions of the dy are from a human. A humanized or fully human form of the antibody can also be prepared in ance with methods well known in the art (see e. g. U.S. Patent No. 5,565,332 to Winter).

Humanization may be ed by various methods including, but not limited to (a) grafting the non-human (e.g., donor antibody) CDRs onto human (e.g. recipient antibody) framework and constant regions with or without retention of critical framework residues (e.g. those that are important for retaining good antigen binding affinity or antibody functions), (b) grafting only the non-human icity-determining regions (SDRs or a-CDRs; the residues critical for the antibody-antigen interaction) onto human ork and constant regions, or (c) transplanting the entire non-human variable domains, but "cloaking" them with a human-like section by replacement of surface residues. Humanized antibodies and methods of making them are reviewed, e.g., in Almagro and Fransson, Front Biosci 13, 1619-1633 (2008), and are further described, e.g., in Riechmann et al., Nature 332, 323-329 (1988); Queen et al., Proc Natl Acad Sci USA 86, 10029-10033 (1989); US Patent Nos. 5,821,337, 7,527,791, 6,982,321, and 7,087,409; Jones et al., Nature 321, 522-525 (1986); Morrison et al., Proc Natl Acad Sci 81, 6851-6855 ; Morrison and Oi, Adv Immunol 44, 65-92 (1988); Verhoeyen et al., Science 239, 1534-1536 (1988); Padlan, Molec Immun 31(3), 169-217 (1994); ri et al., Methods 36, 25-34 (2005) (describing SDR (a-CDR) grafting); Padlan, Mol Immunol 28, 8 (1991) (describing “resurfacing”); Dall’Acqua et al., Methods 36, 43-60 (2005) (describing “FR shuffling”); and Osbourn et al., Methods 36, 61-68 (2005) and Klimka et al., Br J Cancer 83, 252-260 (2000) (describing the “guided selection” approach to FR shuffling). Human antibodies and human le regions can be ed using various techniques known in the art. Human antibodies are bed generally in van Dijk and van de Winkel, Curr Opin Pharmacol 5, 368- 74 (2001) and Lonberg, Curr Opin Immunol 20, 450-459 (2008). Human variable s can form part of and be derived from human onal antibodies made by the hybridoma method (see e.g. Monoclonal Antibody Production Techniques and Applications, pp. 51-63 (Marcel Dekker, Inc., New York, 1987)). Human antibodies and human variable regions may also be prepared by administering an immunogen to a transgenic animal that has been modified to produce intact human antibodies or intact antibodies with human variable regions in response to antigenic challenge (see e.g. Lonberg, Nat Biotech 23, 1117-1125 (2005). Human antibodies and human variable regions may also be generated by isolating Fv clone variable region sequences selected from human-derived phage display libraries (see e.g., Hoogenboom et al. in Methods in Molecular Biology 178, 1-37 (O’Brien et al., ed., Human Press, Totowa, NJ, 2001); and McCafferty et al., Nature 348, 552-554; Clackson et al., Nature 352, 624-628 (1991)). Phage typically display antibody fragments, either as single-chain Fv (scFv) fragments or as Fab nts.

In certain embodiments, the Fab fragments useful herein are ered to have enhanced binding affinity according to, for e, the s disclosed in U.S. Pat. Appl. Publ. No. 2004/0132066, the entire contents of which are hereby incorporated by reference. The ability of the bispecific antigen binding molecule described to bind to a specific antigenic determinant can be measured either through an enzyme-linked immunosorbent assay (ELISA) or other techniques familiar to one of skill in the art, e.g. surface plasmon nce technique zed on a BIACORE T100 system) (Liljeblad, et al., Glyco J 17, 323-329 (2000)), and ional binding assays (Heeley, Endocr Res 28, 9 ). Competition assays may be used to identify an antibody, antibody fragment, antigen binding domain or variable domain that competes with a reference antibody for binding to a particular antigen. In certain embodiments, such a competing antibody binds to the same e (e.g. a linear or a conformational epitope) that is bound by the reference antibody. ed exemplary methods for mapping an epitope to which an antibody binds are provided in Morris (1996) “Epitope Mapping Protocols,” in Methods in Molecular Biology vol. 66 (Humana Press, Totowa, NJ). In an exemplary ition assay, immobilized antigen is incubated in a solution comprising a first labeled antibody that binds to the n and a second unlabeled antibody that is being tested for its ability to e with the first antibody for binding to the antigen. The second antibody may be present in a hybridoma supernatant. As a control, immobilized antigen is incubated in a on comprising the first labeled antibody but not the second unlabeled antibody. After tion under conditions permissive for binding of the first antibody to the antigen, excess unbound antibody is removed, and the amount of label associated with immobilized antigen is measured. If the amount of label associated with immobilized antigen is substantially reduced in the test sample ve to the control sample, then that indicates that the second antibody is competing with the first dy for binding to the antigen. See Harlow and Lane (1988) Antibodies: A Laboratory Manual ch.14 (Cold Spring Harbor Laboratory, Cold Spring Harbor, NY). ific antigen binding molecules prepared as described herein may be purified by art-known ques such as high performance liquid chromatography, ion exchange chromatography, gel electrophoresis, affinity chromatography, size ion chromatography, and the like. The actual conditions used to purify a particular protein will depend, in part, on factors such as net charge, hydrophobicity, hydrophilicity etc., and will be apparent to those having skill in the art.

For affinity chromatography purification an antibody, ligand, receptor or antigen can be used to which the bispecific antigen binding molecule binds. For example, for affinity chromatography purification of bispecific antigen binding molecules described, a matrix with protein A or protein G may be used. Sequential Protein A or G affinity chromatography and size exclusion chromatography can be used to e a bispecific n binding molecule essentially as described in the Examples. The purity of the bispecific antigen binding molecule can be determined by any of a y of well known analytical methods including gel electrophoresis, high pressure liquid chromatography, and the like. For example, the heavy chain fusion proteins expressed as described in the Examples were shown to be intact and ly assembled as demonstrated by reducing SDS-PAGE (see e.g. Figure 3). Three bands were resolved at approximately Mr 25,000, Mr 50,000 and Mr 75,000, corresponding to the predicted molecular weights of the bispecific antigen binding molecule light chains, heavy chain and heavy chain/Fab heavy chain fusion protein, respectively.

Assays Bispecific antigen binding molecules bed herein may be fied, screened for, or terized for their al/chemical properties and/or biological activities by various assays known in the art.

Affinity assays The affinity of the bispecific antigen binding molecule for an Fc receptor or a target antigen can be ined in accordance with the methods set forth in the Examples by surface plasmon resonance (SPR), using standard instrumentation such as a BIAcore instrument (GE Healthcare), and receptors or target proteins such as may be obtained by recombinant expression.

Alternatively, g of bispecific antigen binding molecules for different receptors or target antigens may be evaluated using cell lines expressing the particular receptor or target antigen, for example by flow cytometry (FACS). A specific illustrative and exemplary embodiment for measuring binding affinity is described in the following and in the Examples below.

According to one embodiment, KD is measured by surface n resonance using a BIACORE® T100 machine (GE Healthcare) at 25 °C.

To analyze the interaction between the Fc-portion and Fc ors, gged recombinant Fcreceptor is captured by an anti-Penta His antibody n) immobilized on CM5 chips and the bispecific constructs are used as analytes. Briefly, carboxymethylated dextran biosensor chips (CM5, GE Healthcare) are activated with N-ethyl-N’-(3-dimethylaminopropyl)-carbodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS) according to the supplier’s instructions.

Anti Penta-His antibody is diluted with 10 mM sodium acetate, pH 5.0, to 40 μg/ml before injection at a flow rate of 5 μl/min to e approximately 6500 response units (RU) of coupled protein. Following the injection of the ligand, 1 M ethanolamine is injected to block unreacted groups. Subsequently the Fc-receptor is captured for 60 s at 4 or 10 nM. For kinetic measurements, old serial dilutions of the bispecific construct (range n 500 nM and 4000 nM) are ed in HBS-EP (GE Healthcare, 10 mM HEPES, 150 mM NaCl, 3 mM EDTA, 0.05 % Surfactant P20, pH 7.4) at 25 °C at a flow rate of 30 μl/min for 120 s.

To ine the affinity to the target antigen, bispecific ucts are captured by an anti human Fab specific antibody (GE Healthcare) that is immobilized on an activated CM5-sensor chip surface as described for the anti Penta-His antibody. The final amount of coupled protein is is approximately 12000 RU. The bispecific constructs are captured for 90 s at 300 nM. The target antigens are passed through the flow cells for 180 s at a concentration range from 250 to 1000 nM with a flowrate of 30 μl/min. The dissociation is monitored for 180 s.

Bulk refractive index differences are corrected for by subtracting the se obtained on reference flow cell. The steady state response was used to derive the dissociation constant KD by non-linear curve fitting of the Langmuir binding isotherm. Association rates (kon) and dissociation rates (koff) are calculated using a simple one-to-one Langmuir binding model RE® T100 Evaluation Software version 1.1.1) by aneously fitting the association and dissociation sensorgrams. The equilibrium dissociation constant (KD) is calculated as the ratio koff/kon. See, e.g., Chen et al., J Mol Biol 293, 865-881 (1999). ty assays Biological activity of the bispecific antigen binding molecules described can be measured by various assays known in the art, ing those described in the es. Biological activities may for example include the induction of proliferation of T cells, the induction of ing in T cells, the induction of sion of activation markers in T cells, the ion of cytokine secretion by T cells, the inhibition of signaling in target cells such as tumor cells or cells of the tumor stroma, the inhibition of proliferation of target cells, the induction of lysis of target cells, and the induction of tumor regression and/or the improvement of survival.

Compositions, Formulations, and Routes of Administration Also described are pharmaceutical compositions comprising any of the bispecific antigen binding molecules described herein, e.g., for use in any of the below therapeutic methods. In one embodiment, a pharmaceutical composition comprises any of the bispecific antigen binding molecules described herein and a pharmaceutically acceptable carrier. In another embodiment, a pharmaceutical composition comprises any of the bispecific antigen binding molecules described herein and at least one additional therapeutic agent, e.g., as described below.

Further described is a method of producing a bispecific antigen binding molecule described in a form suitable for administration in vivo, the method comprising (a) obtaining a bispecific antigen binding molecule bed, and (b) formulating the bispecific antigen binding molecule with at least one pharmaceutically acceptable carrier, whereby a preparation of bispecific antigen binding molecule is formulated for administration in vivo.

Pharmaceutical compositions bed comprise a therapeutically effective amount of one or more bispecific antigen binding le dissolved or sed in a pharmaceutically acceptable carrier. The phrases "pharmaceutical or cologically acceptable" refers to molecular es and compositions that are generally non-toxic to recipients at the dosages and concentrations employed, i.e. do not produce an adverse, ic or other untoward reaction when stered to an animal, such as, for example, a human, as appropriate. The preparation of a pharmaceutical composition that contains at least one bispecific antigen binding molecule and optionally an additional active ingredient will be known to those of skill in the art in light of the present disclosure, as exemplified by Remington's Pharmaceutical Sciences, 18th Ed. Mack Printing Company, 1990, incorporated herein by reference. Moreover, for animal (e.g., human) administration, it will be understood that preparations should meet sterility, pyrogenicity, general safety and purity standards as required by FDA Office of Biological Standards or corresponding authorities in other countries. Preferred compositions are lyophilized ations or s solutions. As used herein, "pharmaceutically acceptable carrier" includes any and all solvents, buffers, dispersion media, coatings, tants, antioxidants, vatives (e.g. antibacterial agents, antifungal agents), isotonic agents, absorption delaying agents, salts, preservatives, antioxidants, proteins, drugs, drug stabilizers, polymers, gels, binders, excipients, disintegration agents, lubricants, ning agents, flavoring agents, dyes, such like materials and combinations thereof, as would be known to one of ordinary skill in the art (see, for example, Remington's Pharmaceutical Sciences, 18th Ed. Mack Printing Company, 1990, pp. 1289-1329, incorporated herein by reference). Except r as any conventional carrier is incompatible with the active ingredient, its use in the therapeutic or pharmaceutical compositions is contemplated.

The composition may se different types of carriers depending on whether it is to be administered in solid, liquid or aerosol form, and whether it need to be sterile for such routes of administration as injection. Bispecific antigen binding molecules described (and any additional therapeutic agent) can be administered intravenously, intradermally, intraarterially, eritoneally, intralesionally, intracranially, intraarticularly, intraprostatically, intrasplenically, intrarenally, intrapleurally, intratracheally, intranasally, intravitreally, intravaginally, intrarectally, intratumorally, intramuscularly, intraperitoneally, subcutaneously, subconjunctivally, intravesicularlly, mucosally, intrapericardially, intraumbilically, cularally, orally, topically, locally, by inhalation (e.g. aerosol inhalation), ion, infusion, continuous on, localized perfusion bathing target cells directly, via a catheter, via a , in cremes, in lipid compositions (e.g. liposomes), or by other method or any ation of the forgoing as would be known to one of ry skill in the art (see, for example, Remington's Pharmaceutical Sciences, 18th Ed. Mack Printing Company, 1990, incorporated herein by reference). Parenteral administration, in particular intravenous injection, is most commonly used for administering polypeptide molecules such as the bispecific antigen binding molecules described.

Parenteral compositions include those designed for administration by injection, e.g. aneous, ermal, esional, intravenous, intraarterial intramuscular, hecal or eritoneal ion. For injection, the bispecific antigen binding molecules described may be formulated in aqueous ons, preferably in physiologically compatible s such as Hanks' solution, Ringer's solution, or physiological saline buffer. The solution may contain formulatory agents such as suspending, stabilizing and/or dispersing agents. Alternatively, the bispecific antigen binding molecules may be in powder form for constitution with a suitable vehicle, e.g., sterile pyrogen-free water, before use. Sterile able solutions are prepared by orating the bispecific antigen g molecules described in the required amount in the appropriate solvent with various of the other ingredients enumerated below, as required. Sterility may be readily accomplished, e.g., by filtration through sterile filtration membranes. Generally, dispersions are prepared by incorporating the various ized active ingredients into a sterile vehicle which contains the basic dispersion medium and/or the other ingredients. In the case of sterile powders for the preparation of sterile injectable ons, suspensions or emulsion, the preferred methods of ation are vacuum-drying or freeze-drying techniques which yield a powder of the active ingredient plus any additional desired ingredient from a previously sterilefiltered liquid medium thereof. The liquid medium should be suitably buffered if necessary and the liquid t first rendered isotonic prior to injection with sufficient saline or glucose. The composition must be stable under the conditions of manufacture and storage, and ved against the contaminating action of microorganisms, such as bacteria and fungi. It will be appreciated that endotoxin contamination should be kept minimally at a safe level, for example, less that 0.5 ng/mg protein. Suitable pharmaceutically acceptable carriers include, but are not limited to: buffers such as phosphate, citrate, and other organic acids; antioxidants including ascorbic acid and methionine; preservatives (such as octadecyldimethylbenzyl ammonium chloride; hexamethonium chloride; benzalkonium chloride; benzethonium chloride; phenol, butyl or benzyl alcohol; alkyl parabens such as methyl or propyl paraben; catechol; resorcinol; cyclohexanol; 3-pentanol; and m-cresol); low molecular weight (less than about 10 residues) polypeptides; proteins, such as serum albumin, gelatin, or immunoglobulins; hydrophilic polymers such as nylpyrrolidone; amino acids such as glycine, glutamine, asparagine, histidine, arginine, or lysine; monosaccharides, disaccharides, and other carbohydrates including glucose, mannose, or dextrins; chelating agents such as EDTA; sugars such as sucrose, mannitol, trehalose or sorbitol; salt-forming counter-ions such as sodium; metal complexes (e.g. Zn-protein complexes); and/or non-ionic surfactants such as polyethylene glycol (PEG). Aqueous injection suspensions may contain compounds which increase the viscosity of the suspension, such as sodium carboxymethyl ose, sorbitol, dextran, or the like. Optionally, the suspension may also contain suitable stabilizers or agents which increase the solubility of the compounds to allow for the preparation of highly concentrated solutions. Additionally, suspensions of the active compounds may be prepared as appropriate oily injection suspensions. Suitable lipophilic solvents or vehicles include fatty oils such as sesame oil, or synthetic fatty acid esters, such as ethyl cleats or triglycerides, or liposomes.

Active ingredients may be entrapped in microcapsules prepared, for example, by coacervation techniques or by interfacial polymerization, for e, hydroxymethylcellulose or gelatinmicrocapsules and poly-(methylmethacylate) microcapsules, respectively, in colloidal drug delivery systems (for example, liposomes, albumin microspheres, microemulsions, nanoparticles and nanocapsules) or in macroemulsions. Such techniques are disclosed in Remington's Pharmaceutical Sciences (18th Ed. Mack Printing Company, 1990). Sustained-release preparations may be ed. Suitable examples of sustained-release preparations include semipermeable matrices of solid hydrophobic polymers containing the polypeptide, which matrices are in the form of shaped articles, e.g. films, or microcapsules. In particular ments, ged absorption of an able composition can be brought about by the use in the compositions of agents delaying tion, such as, for example, aluminum monostearate, gelatin or combinations thereof.

In addition to the compositions described previously, the bispecific antigen binding molecules may also be formulated as a depot preparation. Such long acting formulations may be administered by implantation (for example subcutaneously or intramuscularly) or by intramuscular injection. Thus, for example, the bispecific antigen g les may be formulated with suitable polymeric or hydrophobic materials (for example as an on in an acceptable oil) or ion exchange resins, or as sparingly e derivatives, for example, as a sparingly soluble salt.

Pharmaceutical compositions comprising the bispecific antigen g molecules described may be ctured by means of conventional mixing, dissolving, emulsifying, encapsulating, entrapping or lyophilizing processes. Pharmaceutical compositions may be formulated in conventional manner using one or more physiologically acceptable carriers, diluents, excipients or auxiliaries which facilitate processing of the proteins into preparations that can be used ceutically. Proper formulation is dependent upon the route of administration chosen.

The bispecific antigen binding molecules may be formulated into a composition in a free acid or base, neutral or salt form. Pharmaceutically acceptable salts are salts that substantially retain the biological activity of the free acid or base. These include the acid addition salts, e.g., those formed with the free amino groups of a proteinaceous composition, or which are formed with inorganic acids such as for example, hydrochloric or oric acids, or such organic acids as acetic, oxalic, ic or mandelic acid. Salts formed with the free carboxyl groups can also be derived from inorganic bases such as for example, sodium, potassium, ammonium, calcium or ferric hydroxides; or such organic bases as isopropylamine, trimethylamine, histidine or procaine. Pharmaceutical salts tend to be more soluble in aqueous and other protic solvents than are the corresponding free base forms.

Therapeutic Methods and Compositions Any of the bispecific antigen binding molecules bed herein may be used in therapeutic methods. Bispecific antigen binding molecules described can be used for example in the treatment of cancers.

For use in therapeutic methods, bispecific antigen binding molecules described would be formulated, dosed, and administered in a fashion tent with good medical practice. s for consideration in this context include the particular disorder being treated, the particular mammal being treated, the clinical condition of the individual patient, the cause of the disorder, the site of delivery of the agent, the method of administration, the scheduling of stration, and other factors known to l practitioners.

Described are bispecific antigen binding molecules described for use as a medicament.Also described arebispecific antigen g molecules described for use in treating a disease. In certain embodiments, bispecific antigen binding molecules described for use in a method of treatment are described. In one embodiment, described is a bispecific antigen binding molecule as described herein for use in the treatment of a e in an individual in need thereof. In n embodiments, described is a bispecific antigen g molecule for use in a method of treating an dual having a disease comprising administering to the individual a therapeutically effective amount of the bispecific antigen binding molecule. In certain embodiments the disease to be d is a proliferative disorder. In a particular embodiment the disease is cancer. In certain embodiments the method further comprises administering to the individual a therapeutically effective amount of at least one additional therapeutic agent, e.g., an anti-cancer agent if the disease to be d is . An “individual” according to any of the above embodiments is a mammal, preferably a human.

Also described is the use of a bispecific antigen binding le described in the manufacture or preparation of a medicament. In one embodiment the medicament is for the treatment of a disease in an individual in need thereof. In a further embodiment, the medicament is for use in a method of ng a e sing administering to an individual having the disease a therapeutically effective amount of the medicament. In certain embodiments the disease to be treated is a proliferative disorder. In a particular embodiment the e is . In one ment, the method further comprises administering to the individual a therapeutically effective amount of at least one additional therapeutic agent, e.g., an anti-cancer agent if the disease to be treated is cancer. An “individual” according to any of the above embodiments may be a mammal, ably a human.

Also described is a method for treating a disease. In one embodiment, the method comprises administering to an individual having such disease a therapeutically effective amount of a bispecific antigen binding molecule described. In one embodiment a composition is administered to said invididual, sing the ific antigen binding molecule described in a pharmaceutically acceptable form. In n embodiments the disease to be treated is a proliferative disorder. In a particular embodiment the disease is cancer. In certain embodiments the method further comprises administering to the individual a therapeutically ive amount of at least one additional therapeutic agent, e.g., an anti-cancer agent if the e to be treated is cancer. An “individual” according to any of the above embodiments may be a mammal, preferably a human.

In certain embodiments the disease to be treated is a proliferative disorder, particularly cancer.

Non-limiting examples of cancers include bladder cancer, brain cancer, head and neck cancer, pancreatic cancer, lung cancer, breast cancer, ovarian cancer, uterine cancer, cervical cancer, endometrial cancer, esophageal cancer, colon , colorectal , rectal cancer, gastric cancer, prostate cancer, blood cancer, skin cancer, squamous cell carcinoma, bone cancer, and kidney cancer. Other cell eration disorders that can be treated using a bispecific antigen binding molecule described include, but are not limited to neoplasms located in the: abdomen, bone, breast, digestive system, liver, pancreas, peritoneum, endocrine glands (adrenal, parathyroid, pituitary, testicles, ovary, thymus, thyroid), eye, head and neck, nervous system (central and peripheral), lymphatic system, pelvic, skin, soft tissue, spleen, thoracic region, and urogenital system. Also included are ncerous conditions or lesions and cancer metastases.

In certain embodiments the cancer is chosen from the group ting of renal cell cancer, skin cancer, lung cancer, ctal cancer, breast , brain cancer, head and neck cancer. A skilled artisan y recognizes that in many cases the bispecific antigen binding molecule may not provide a cure but may only provide partial benefit. In some embodiments, a physiological change having some benefit is also considered therapeutically beneficial. Thus, in some embodiments, an amount of bispecific antigen binding molecule that provides a physiological change is ered an "effective amount" or a "therapeutically effective amount". The subject, patient, or individual in need of treatment is lly a mammal, more specifically a human.

In some embodiments, an effective amount of a bispecific antigen binding le described is administered to a cell. In other embodiments, a eutically effective amount of a bispecific antigen binding molecule described is administered to an individual for the treatment of disease.

For the prevention or treatment of disease, the appropriate dosage of a bispecific n binding molecule bed (when used alone or in combination with one or more other additional therapeutic agents) will depend on the type of e to be treated, the route of administration, the body weight of the patient, the type of bispecific antigen binding molecule, the severity and course of the disease, whether the bispecific n binding le is administered for preventive or therapeutic purposes, previous or concurrent therapeutic interventions, the patient's clinical history and response to the ific antigen binding molecule, and the discretion of the attending physician. The practitioner responsible for administration will, in any event, determine the concentration of active ingredient(s) in a composition and appropriate dose(s) for the individual subject. Various dosing schedules including but not limited to single or multiple administrations over various oints, bolus administration, and pulse infusion are contemplated herein.

The bispecific antigen binding molecule is suitably administered to the patient at one time or over a series of treatments. Depending on the type and ty of the disease, about 1 µg/kg to mg/kg (e.g. 0.1 mg/kg – 10 mg/kg) of ific antigen g molecule can be an initial candidate dosage for administration to the patient, whether, for example, by one or more separate administrations, or by continuous infusion. One typical daily dosage might range from about 1 µg/kg to 100 mg/kg or more, depending on the factors mentioned above. For repeated administrations over several days or longer, depending on the condition, the treatment would generally be sustained until a desired suppression of disease symptoms occurs. One exemplary dosage of the bispecific antigen binding molecule would be in the range from about 0.005 mg/kg to about 10 mg/kg. In other non-limiting examples, a dose may also comprise from about 1 microgram/kg body , about 5 microgram/kg body weight, about 10 microgram/kg body weight, about 50 microgram/kg body weight, about 100 microgram/kg body , about 200 microgram/kg body weight, about 350 microgram/kg body weight, about 500 microgram/kg body weight, about 1 milligram/kg body weight, about 5 milligram/kg body weight, about 10 milligram/kg body weight, about 50 milligram/kg body weight, about 100 milligram/kg body weight, about 200 milligram/kg body weight, about 350 milligram/kg body weight, about 500 milligram/kg body weight, to about 1000 mg/kg body weight or more per administration, and any range derivable therein. In non-limiting es of a derivable range from the numbers listed , a range of about 5 mg/kg body weight to about 100 mg/kg body weight, about 5 microgram/kg body weight to about 500 milligram/kg body weight, etc., can be administered, based on the numbers described above. Thus, one or more doses of about 0.5 mg/kg, 2.0 mg/kg, .0 mg/kg or 10 mg/kg (or any combination thereof) may be administered to the patient. Such doses may be stered intermittently, e.g. every week or every three weeks (e.g. such that the patient es from about two to about twenty, or e.g. about six doses of the bispecific antigen binding molecule). An initial higher loading dose, followed by one or more lower doses may be stered. However, other dosage regimens may be useful. The progress of this therapy is easily monitored by conventional techniques and .

The bispecific antigen binding molecules described will generally be used in an amount effective to e the ed purpose. For use to treat or prevent a disease condition, the bispecific antigen binding les described, or pharmaceutical compositions thereof, are administered or d in a therapeutically effective amount. Determination of a therapeutically effective amount is well within the capabilities of those skilled in the art, especially in light of the detailed disclosure provided .

For systemic administration, a therapeutically effective dose can be estimated initially from in vitro assays, such as cell culture assays. A dose can then be ated in animal models to achieve a circulating concentration range that includes the IC50 as determined in cell culture.

Such information can be used to more accurately determine useful doses in humans.

Initial dosages can also be estimated from in vivo data, e.g., animal models, using techniques that are well known in the art. One having ordinary skill in the art could readily optimize administration to humans based on animal data.

Dosage amount and interval may be adjusted individually to provide plasma levels of the bispecific antigen binding molecules which are sufficient to maintain therapeutic effect. Usual patient dosages for administration by injection range from about 0.1 to 50 mg/kg/day, lly from about 0.5 to 1 mg/kg/day. Therapeutically effective plasma levels may be achieved by administering multiple doses each day. Levels in plasma may be ed, for e, by HPLC.

In cases of local administration or selective uptake, the effective local concentration of the bispecific antigen binding molecules may not be related to plasma concentration. One having skill in the art will be able to optimize therapeutically effective local dosages without undue experimentation.

A therapeutically effective dose of the bispecific antigen binding molecules bed herein will generally provide therapeutic benefit without g substantial ty. ty and therapeutic efficacy of a bispecific antigen binding molecule can be determined by standard pharmaceutical procedures in cell culture or experimental animals. Cell culture assays and animal studies can be used to determine the LD50 (the dose lethal to 50% of a population) and the ED50 (the dose therapeutically effective in 50% of a population). The dose ratio between toxic and therapeutic s is the therapeutic index, which can be expressed as the ratio LD50/ED50.

Bispecific n binding molecules that exhibit large therapeutic indices are preferred. In one embodiment, the ific antigen binding molecule described exhibits a high therapeutic index.

The data obtained from cell culture assays and animal studies can be used in formulating a range of dosages suitable for use in humans. The dosage lies preferably within a range of circulating concentrations that include the ED50 with little or no toxicity. The dosage may vary within this range depending upon a variety of s, e.g., the dosage form ed, the route of administration utilized, the condition of the subject, and the like. The exact formulation, route of administration and dosage can be chosen by the individual physician in view of the patient's condition (see, e.g., Fingl et al., 1975, in: The Pharmacological Basis of Therapeutics, Ch. 1, p. 1, incorporated herein by reference in its entirety).

The attending physician for ts treated with bispecific antigen binding molecules described would know how and when to terminate, interrupt, or adjust administration due to toxicity, organ dysfunction, and the like. Conversely, the attending physician would also know to adjust treatment to higher levels if the al response were not adequate (precluding toxicity). The magnitude of an administered dose in the ment of the disorder of interest will vary with the severity of the condition to be treated, with the route of administration, and the like. The severity of the condition may, for example, be evaluated, in part, by standard stic evaluation methods. r, the dose and perhaps dose frequency will also vary according to the age, body weight, and response of the individual patient.

Other Agents and Treatments The bispecific antigen binding molecules described may be administered in ation with one or more other agents in therapy. For instance, a bispecific antigen binding molecule described may be co-administered with at least one additional therapeutic agent. The term "therapeutic agent” encompasses any agent administered to treat a symptom or disease in an individual in need of such treatment. Such additional therapeutic agent may comprise any active ingredients suitable for the particular indication being treated, preferably those with complementary activities that do not adversely affect each other. In certain embodiments, an additional therapeutic agent is an immunomodulatory agent, a cytostatic agent, an inhibitor of cell adhesion, a cytotoxic agent, an activator of cell apoptosis, or an agent that increases the sensitivity of cells to apoptotic inducers. In a particular embodiment, the additional therapeutic agent is an anti-cancer agent, for e a microtubule disruptor, an antimetabolite, a topoisomerase tor, a DNA intercalator, an alkylating agent, a al therapy, a kinase tor, a receptor antagonist, an tor of tumor cell apoptosis, or an giogenic agent.

Such other agents are suitably present in combination in s that are effective for the purpose intended. The effective amount of such other agents depends on the amount of ific n binding molecule used, the type of disorder or treatment, and other factors discussed above. The bispecific antigen binding molecules are generally used in the same dosages and with administration routes as described herein, or about from 1 to 99% of the dosages described herein, or in any dosage and by any route that is empirically/clinically determined to be appropriate.

Such combination ies noted above encompass combined administration (where two or more therapeutic agents are included in the same or separate compositions), and separate administration, in which case, administration of the bispecific antigen binding molecule described can occur prior to, simultaneously, and/or following, administration of the additional therapeutic agent and/or adjuvant. Bispecific antigen binding molecules described can also be used in combination with radiation y.

Articles of Manufacture Also described is, an article of manufacture containing materials useful for the treatment, prevention and/or sis of the disorders described above. The article of manufacture comprises a container and a label or package insert on or associated with the container. le containers include, for e, bottles, vials, syringes, IV solution bags, etc. The containers may be formed from a variety of materials such as glass or plastic. The container holds a composition which is by itself or combined with another composition effective for treating, preventing and/or diagnosing the condition and may have a e access port (for example the container may be an intravenous solution bag or a vial having a stopper pierceable by a hypodermic injection needle). At least one active agent in the composition is a bispecific antigen binding molecule described. The label or package insert indicates that the composition is used for treating the condition of choice. Moreover, the article of manufacture may comprise (a) a first container with a composition contained therein, wherein the ition comprises a bispecific antigen binding molecule described; and (b) a second container with a composition contained therein, wherein the composition comprises a further cytotoxic or otherwise therapeutic agent.

The e of manufacture in this embodiment may further comprise a package insert indicating that the compositions can be used to treat a particular condition. Alternatively, or additionally, the article of manufacture may further comprise a second (or third) container comprising a ceutically-acceptable buffer, such as bacteriostatic water for injection , phosphate-buffered saline, Ringer's on and dextrose solution. It may further include other materials desirable from a commercial and user standpoint, including other buffers, diluents, s, needles, and syringes.

Examples The following are examples of methods and compositions described. It is understood that various other ments may be practiced, given the general ption provided above. l methods inant DNA Techniques Standard methods were used to manipulate DNA as described in ok et al., Molecular cloning: A laboratory manual; Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York, 1989. The molecular biological reagents were used according to the manufacturers’ instructions. General information regarding the nucleotide sequences of human immunoglobulins light and heavy chains is given in: Kabat, E.A. et al., (1991) ces of Proteins of Immunological Interest, 5th ed., NIH Publication No. 91-3242.

DNA Sequencing DNA sequences were determined by double strand sequencing.

Gene Synthesis Desired gene segments where required were either generated by PCR using appropriate templates or were synthesized by Geneart AG (Regensburg, Germany) from synthetic oligonucleotides and PCR products by automated gene synthesis. In cases where no exact gene sequence was available, oligonucleotide primers were designed based on sequences from closest homologues and the genes were ed by RT-PCR from RNA originating from the riate tissue. The gene segments flanked by singular restriction endonuclease cleavage sites were cloned into standard cloning / sequencing s. The plasmid DNA was purified from transformed bacteria and concentration determined by UV spectroscopy. The DNA sequence of the ned gene fragments was confirmed by DNA sequencing. Gene segments were designed with suitable restriction sites to allow sub-cloning into the respective expression vectors. All constructs were designed with a 5’-end DNA sequence coding for a leader peptide which s proteins for ion in eukaryotic cells. SEQ ID NOs 74-76 give exemplary leader peptides.

Isolation of primary human pan T cells from PBMCs Peripheral blood mononuclear cells (PBMCs) were prepared by Histopaque density centrifugation from enriched lymphocyte preparations (buffy coats) ed from local blood banks or from fresh blood from healthy human donors. Briefly, blood was d with sterile PBS and carefully layered over a aque gradient (Sigma, H8889). After centrifugation for minutes at 450 x g at room temperature (brake switched off), part of the plasma above the PBMC containing interphase was discarded. The PBMCs were transferred into new 50 ml Falcon tubes and tubes were filled up with PBS to a total volume of 50 ml. The mixture was centrifuged at room temperature for 10 minutes at 400 x g (brake switched on). The supernatant was discarded and the PBMC pellet washed twice with sterile PBS (centrifugation steps at 4°C for 10 minutes at 350 x g). The ing PBMC tion was d automatically (ViCell) and stored in RPMI1640 medium, containing 10% FCS and 1% L-alanyl-L-glutamine (Biochrom, K0302) at 37°C, 5% CO2 in the incubator until assay start.

T cell enrichment from PBMCs was performed using the Pan T Cell Isolation Kit II (Miltenyi Biotec #130156), ing to the manufacturer’s instructions. Briefly, the cell pellets were d in 40 µl cold buffer per 10 million cells (PBS with 0.5% BSA, 2 mM EDTA, sterile filtered) and incubated with 10 µl Biotin-Antibody Cocktail per 10 million cells for 10 min at 4°C. 30 µl cold buffer and 20 µl Anti-Biotin magnetic beads per 10 million cells were added, and the mixture incubated for another 15 min at 4°C. Cells were washed by adding 10-20x the current volume and a subsequent fugation step at 300 x g for 10 min. Up to 100 n cells were ended in 500 µl buffer. Magnetic separation of unlabeled human pan T cells was performed using LS columns (Miltenyi Biotec #130401) according to the manufacturer’s instructions. The resulting T cell population was d automatically (ViCell) and stored in AIM-V medium at 37°C, 5% CO2 in the incubator until assay start (not longer than 24 h). ion of primary human naive T cells from PBMCs Peripheral blood mononuclar cells (PBMCs) were prepared by Histopaque density centrifugation from enriched lymphocyte preparations (buffy coats) obtained from local blood banks or from fresh blood from healthy human donors. T-cell enrichment from PBMCs was performed using the Naive CD8+ T cell isolation Kit from Miltenyi Biotec (#130244), according to the manufacturer’s instructions, but skipping the last isolation step of CD8+ T cells (also see description for the isolation of primary human pan T cells).

Isolation of primary cynomolgus PBMCs from heparinized blood eral blood mononuclar cells (PBMCs) were prepared by density centrifugation from fresh blood from healthy cynomolgus donors, as follows: Heparinized blood was diluted 1:3 with sterile PBS, and Lymphoprep medium (Axon Lab #1114545) was diluted to 90% with sterile PBS. Two volumes of the diluted blood were layered over one volume of the diluted density gradient and the PBMC fraction was ted by centrifugation for 30 min at 520 x g, without brake, at room temperature. The PBMC band was transferred into a fresh 50 ml Falcon tube and washed with e PBS by centrifugation for 10 min at 400 x g at 4°C. One low-speed centrifugation was performed to remove the platelets (15 min at 150 x g, 4°C), and the resulting PBMC population was automatically counted (ViCell) and immediately used for further assays.

Target cells For the assessment of MCSP-targeting bispecific antigen binding molecules, the following tumor cell lines were used: the human melanoma cell line WM266-4 (ATCC 676), derived from a metastatic site of a malignant melanoma and expressing high levels of human MCSP; and the human melanoma cell line MV-3 (a kind gift from The Radboud University Nijmegen Medical Centre), expressing medium levels of human MCSP.

For the ment of rgeting bispecific antigen binding molecules, the following tumor cell lines were used: the human gastric cancer cell line MKN45 (DSMZ #ACC 409), expressing very high levels of human CEA; the human female Caucasian colon adenocarcinoma cell line LS-174T (ECACC #87060401), expressing medium to low levels of human CEA; the human lioid pancreatic carcinoma cell line Panc-1 (ATCC 469), expressing (very) low levels of human CEA; and a murine colon carcinoma cell line MC38-huCEA, that was engineered in-house to stably express human CEA.

In addition, a human T cell leukaemia cell line, Jurkat (ATCC #TIB-152), was used to assess binding of ent bispecific constructs to human CD3 on cells.

Example 1 ation, purification and characterization of bispecific antigen binding molecules The heavy and light chain le region DNA sequences were subcloned in frame with either the constant heavy chain or the constant light chain pre-inserted into the respective recipient mammalian expression vector. The antibody expression was driven by an MPSV promoter and a synthetic polyA signal sequence is located at the 3’ end of the CDS. In addition each vector contained an EBV OriP sequence.

The molecules were produced by co-transfecting HEK293 EBNA cells with the mammalian expression vectors. Exponentially growing HEK293 EBNA cells were transfected using the calcium phosphate method. Alternatively, HEK293 EBNA cells growing in suspension were transfected using polyethylenimine (PEI). For preparation of “1+1 IgG Crossfab” constructs, cells were transfected with the corresponding expression vectors in a 1:1:1:1 ratio (“vector second heavy chain” : r first light chain” : “vector light chain Crossfab” : “vector first heavy chain-heavy chain ab”). For preparation of “2+1 IgG ab” constructs cells were transfected with the corresponding expression s in a 1:2:1:1 ratio (“vector second heavy chain” : “vector light chain” : “vector first heavy chain-heavy chain Crossfab” : “vector light chain Crossfab”. For preparation of the “2+1 IgG Crossfab (N-terminal), linked light chain” construct, cells were transfected with the corresponding expression vectors in a 1:1:1:1 ratio (“vector heavy chain” : “vector light chain” : “vector heavy chain (CrossFab-Fab-Fc)” : “vector linked light chain”). For preparation of the “1+1 CrossMab” construct, cells were transfected with the corresponding expression vectors in a 1:1:1:1 ratio (“vector first heavy chain” : “vector second heavy chain” : “vector first light chain” : “vector second light chain”).

For transfection using m phosphate cells were grown as adherent monolayer cultures in T- flasks using DMEM culture medium supplemented with 10 % (v/v) FCS, and transfected when they were between 50 and 80 % confluent. For the transfection of a T150 flask, 15 million cells were seeded 24 hours before transfection in 25 ml DMEM culture medium supplemented with FCS (at 10% v/v final), and cells were placed at 37°C in an incubator with a 5% CO2 atmosphere overnight. For each T150 flask to be transfected, a solution of DNA, CaCl2 and water was prepared by mixing 94 µg total plasmid vector DNA divided in the corresponding ratio, water to a final volume of 469 µl and 469 µl of a 1 M CaCl2 solution. To this solution, 938 µl of a 50 mM HEPES, 280 mM NaCl, 1.5 mM Na2HPO4 solution at pH 7.05 were added, mixed ately for 10 s and left to stand at room temperature for 20 s. The suspension was d with 10 ml of DMEM supplemented with 2 % (v/v) FCS, and added to the T150 in place of the ng medium. Subsequently, onal 13 ml of transfection medium were added. The cells were incubated at 37°C, 5% CO2 for about 17 to 20 hours, then medium was replaced with 25 ml DMEM, 10 % FCS. The conditioned culture medium was harvested approximately 7 days dia exchange by centrifugation for 15 min at 210 x g, sterile filtered (0.22  m filter), supplemented with sodium azide to a final concentration of 0.01 % (w/v), and kept at 4°C.

For transfection using polyethylenimine (PEI) HEK293 EBNA cells were cultivated in suspension in serum free CD CHO e medium. For the production in 500 ml shake , 400 million HEK293 EBNA cells were seeded 24 hours before transfection. For transfection cells were centrifuged for 5 min at 210 x g, and supernatant was replaced by 20 ml pre-warmed CD CHO medium. sion vectors were mixed in 20 ml CD CHO medium to a final amount of 200 μg DNA. After addition of 540 μl PEI, the mixture was vortexed for 15 s and subsequently incubated for 10 min at room ature. Afterwards cells were mixed with the DNA/PEI on, transferred to a 500 ml shake flask and incubated for 3 hours at 37°C in an tor with a 5% CO2 atmosphere. After the tion time 160 ml F17 medium was added and cells were cultivated for 24 hours. One day after ection 1 mM valproic acid and 7% Feed 1 (Lonza) were added. After a cultivation of 7 days, supernatant was collected for cation by centrifugation for 15 min at 210 x g, the solution was sterile filtered (0.22 μm filter), supplemented with sodium azide to a final concentration of 0.01 % w/v, and kept at 4°C.

The secreted proteins were purified from cell culture supernatants by Protein A affinity chromatography, followed by a size exclusion tography step.

For affinity chromatography supernatant was loaded on a HiTrap nA HP column (CV = 5 ml, GE Healthcare) equilibrated with 25 ml 20 mM sodium phosphate, 20 mM sodium citrate, pH 7.5 or 40 ml 20 mM sodium phosphate, 20 mM sodium citrate, 0.5 M sodium chloride, pH 7.5. Unbound protein was removed by g with at least ten column volumes 20 mM sodium phosphate, 20 mM sodium citrate, 0.5 M sodium chloride pH 7.5, followed by an additional wash step using six column volumes 10 mM sodium phosphate, 20 mM sodium citrate, 0.5 M sodium de pH 5.45. Subsequently, the column was washed with 20 ml 10 mM MES, 100 mM sodium chloride, pH 5.0, and target protein was eluted in six column volumes 20 mM sodium citrate, 100 mM sodium chloride, 100 mM glycine, pH 3.0. Alternatively, target protein was eluted using a gradient over 20 column volumes from 20 mM sodium citrate, 0.5 M sodium chloride, pH 7.5 to 20 mM sodium citrate, 0.5 M sodium chloride, pH 2.5. The n solution was neutralized by adding 1/10 of 0.5 M sodium phosphate, pH 8. The target protein was concentrated and filtrated prior to loading on a HiLoad Superdex 200 column (GE Healthcare) equilibrated with 25 mM potassium phosphate, 125 mM sodium chloride, 100 mM glycine solution of pH 6.7. For the purification of 1+1 IgG Crossfab the column was equilibrated with 20 mM histidine, 140 mM sodium chloride solution of pH 6.0.

The protein concentration of purified protein samples was determined by measuring the optical density (OD) at 280 nm, using the molar extinction coefficient calculated on the basis of the amino acid sequence. Purity and molecular weight of the bispecific ucts were analyzed by SDS-PAGE in the presence and absence of a reducing agent (5 mM 1,4-dithiotreitol) and staining with Coomassie (SimpleBlue™ SafeStain from Invitrogen), using the NuPAGE® Pre- Cast gel system (Invitrogen, USA) according to the manufacturer’s ctions (4-12% Tris- Acetate gels or 4-12% Bis-Tris). Alternatively, purity and molecular weight of molecules were analyzed by CE-SDS analyses in the presence and absence of a reducing agent, using the Caliper LabChip GXII system (Caliper Lifescience) according to the manufacturer’s instructions.

The ate content of the protein samples was ed using a Superdex 200 10/300GL analytical size-exclusion chromatography column (GE Healthcare) in 2 mM MOPS, 150 mM NaCl, 0.02% (w/v) NaN3, pH 7.3 running buffer at 25°C. Alternatively, the aggregate t of dy samples was analyzed using a TSKgel G3000 SW XL analytical size-exclusion column (Tosoh) in 25 mM K2HPO4, 125 mM NaCl, 200 mM L-arginine monohydrocloride, 0.02% (w/v) NaN3, pH 6.7 running buffer at 25°C.

The “2+1 IgG Crossfab (C-terminal)” construct (see SEQ ID NOs 11, 12, 13 and 14) was prepared using plasmid vectors having a genomic gene zation, including intron sequences, a CMV promoter and a polyadenylation signal from the gene of bovine growth hormone. The bispecific construct was transiently expressed in F cells by simultaneous transfection of ed plasmids via ction using different plasmid ratios. Cell were grown in F17 medium.

Supernatant was collected 5-7 days after transfection. Harvested cell-culture supernatant was sterile filtrated through a 0.2 μm pore-size membrane (Millipore) prior to purification. For purification, the bispecific molecule was ed on a MabSelectSure resin (GE Healthcare), washed with 1x PBS and eluted with 20 mM sodium-citrate at pH 3.0. The molecule was further purified by size exclusion chromatography using a SuperdexTM 200 GL ham Bioscience) column equilibrated with 20 mM histidine, 140 mM NaCl, pH 6.0. Characterization (antibody integrity assessment) of the bispecific molecule was done using Capillary electrophoresis (CESDS ) analysis, using microfluidic Labchip technology (Caliper). 5 µl of protein solution was prepared for CE-SDS analysis using the HT Protein Express Reagent Kit according to the manufacturer’s instructions and analysed on a p GXII system using a HT Protein Express Chip.

Figures 2-5 show the results of the SDS PAGE and analytical size exclusion chromatography and Table 2 shows the , aggregate content after Protein A and final monomer content of the preparations of the different ific constructs. antly, the bispecific IgG Crossfab constructs described showed a 10 to 20 fold reduced aggregate t after Protein A affinity chromatography as compared to corresponding bispecific constructs comprising a single chain Fab fragment instead of a Crossfab fragment (data not shown).

Figure 13 shows the result of the CE-SDS analyses of the anti-CD3/anti-MCSP bispecific “2+1 IgG Crossfab (N-terminal), linked light chain” construct (see SEQ ID NOs 1, 4, 5 and 85). 2 µg sample was used for analyses. Figure 14 shows the result of the analytical size exclusion chromatography of the final product (20 µg sample ed).

Figure 20 shows the results of the CE-SDS analyses of the 1+1 IgG Crossfab minal); VL/VH exchange (LC007/V9), the 1+1 CrossMab; CL/CH1 exchange /V9), the 2+1 IgG Crossfab (N-terminal), ed; CL/CH1 ge (LC007/V9), the 2+1 IgG Crossfab (N- terminal); VL/VH exchange (M4-3 ML2/V9), the 2+1 IgG Crossfab (N-terminal); CL/CH1 exchange (M4-3 ML2/V9), and the 2+1 IgG Crossfab (N-terminal), inverted; CL/CH1 exchange (CH1A1A/V9), and Table 2 shows the yields, aggregate content after Protein A and final monomer content of the preparations of the different bispecific constructs.

TABLE 2. Yields, aggregate content after Protein A and final and monomer content.

Construct Yield Aggregates after HMW LMW r [mg/l] n A [%] [%] [%] [%] 2+1 IgG Crossfab (N-terminal); 12.8 2.2 0 0 100 VL/VH exchange (MCSP (LC007)/huCD3) 2+1 IgG Crossfab (N-terminal); 3.2 5.7 0.4 0 99.6 VL/VH exchange (MCSP (LC007)/cyCD3) 1+1 IgG Crossfab (N-terminal); 9.8 0 0 0 100 VL/VH exchange (MCSP (LC007)/huCD3) 2+1 IgG Crossfab (N-terminal), 0.34 13.04 4.4 0 95.6 ed; VL/VH exchange uCD3) 2+1 IgG Crossfab (C-terminal); 15 14 CL/CH1 exchange (c-Met/Her3) 2+1 IgG Crossfab (N-terminal), 0.54 40 1.4 0 98.6 linked light chain; VL/VH exchange (MCSP (LC007)/huCD3) 1+1 IgG ab (N-terminal); 6.61 8.5 0 0 100 VL/VH exchange (MCSP (LC007)/huCD3) 1+1 CrossMab; CL/CH1 6.91 10.5 1.3 1.7 97 exchange (MCSP (LC007)/huCD3) 2+1 IgG Crossfab (N-terminal), 9.45 6.1 0.8 0 99.2 inverted; CL/CH1 exchange (MCSP (LC007)/huCD3 2+1 IgG Crossfab minal); 36.6 0 9.5 35.3 55.2 VL/VH exchange (MCSP (M4-3 ML2)/huCD3) 2+1 IgG Crossfab (N-terminal); 2.62 12 2.8 0 97.2 CL/CH1 exchange (MCSP(M4-3 uCD3) 2+1 IgG ab (N-terminal), 12.7 43 0 0 100 inverted; CL/CH1 exchange (CEA/huCD3) Example 2 Simultaneous g of bispecific constructs to both target antigens Simultaneous binding to of the “2+1 IgG Crossfab minal)” construct (SEQ ID NOs 1, 3, 4, ) to human MCSP and human CD3ε was analyzed by surface plasmon resonance (Figure 6).

All surface n resonance (SPR) experiments are performed on a Biacore T100 at 25°C with HBS-EP as running buffer (0.01 M HEPES pH 7.4, 0.15 M NaCl, 3 mM EDTA, 0.005% Surfactant P20, Biacore, Freiburg/Germany).

Analysis of simultaneous g of the bispecific construct to the tumor antigen and the human CD3ε was performed by direct coupling of 1650 resonance units (RU) of biotinylated D3 domain of MCSP on a sensor chip SA using the standard coupling procedure. The assay setup is shown in Figure 6A.

The “2+1 IgG Crossfab (N-terminal)” construct was captured for 60 s at 200 nM. Human CD3γ(G4S)5CD3ε–AcTev–Fc(knob)–Avi/Fc(hole) was subsequently passed at a concentration of 2000 nM and a flow rate of 40 μl/min for 60 s. Bulk refractive index differences were corrected for by subtracting the response obtained on a nce flow cell where the recombinant CD3ε was flown over a surface with immobilized D3 domain of MCSP without captured bispecific uct.

As shown in Figure 6B, the construct was able to bind the tumor antigen and the CD3 simultaneously. The binding level (RU) after injection of human CD3ε was higher than the binding level achieved after injection of the construct alone reflecting that both tumor antigen and the human CD3ε are bound to the bispecific construct.

Example 3 T cell activation by bispecific constructs in the presence and absence of target cells Cytokine release The purified “2+1 IgG Crossfab minal)” construct (SEQ ID NOs 1, 3, 4, 5) and the “(scFv)2”molecule, both ing human MCSP and human CD3, were analyzed for their y to induce T cell-mediated de novo secretion of cytokines in the presence or absence of tumor target cells. y, 280 µl whole blood from a healthy donor were plated per well of a deep-well 96-well plate. 30 000 Colo-38 tumor target cells, expressing human MCSP, as well as the two bispecific constructs and IgG controls were added at 1 nM final concentration. The cells were incubated for 24 h at 37°C, 5% CO2 and then centrifuged for 5 min at 350 x g. The supernatant was transferred into a new deep-well 96-well-plate for the subsequent is. The CBA analysis was med according to manufacturer’s instructions for FACS CantoII, using the combination of the ing CBA Flex Sets: human granzyme B (BD 4), human IFN-γ Flex Set (BD #558269), human TNF Flex Set (BD #558273), human IL-10 Flex Set (BD #558274), human IL- 6 Flex Set (BD #558276), human IL-4 Flex Set (BD #558272), human IL-2 Flex Set (BD #558270).

Figure 7 shows the levels of the different cytokine measured in the atant. The main cytokine secreted in the presence of Colo-38 tumor cells was IL-6, followed by IFN-γ. In addition, also the levels of granzyme B strongly increased upon activation of T cells in the presence of target cells. In general, both bispecific constructs induced high levels of cytokine secretion in the presence of target cells (Figure 7, A and B), but not in the absence of target cells (Figure 7, C and D). There was no icant ion of Th2 cytokines (IL-10 and IL-4) upon activation of T cells in the presence (or absence) of target cells.

Expression of surface activation markers In another experiment, purified “2+1 IgG Crossfab (N-terminal)” (SEQ ID NOs 4, 5, 6, 7), targeting cynomolgus CD3 and human MCSP, was analyzed for its potential to up-regulate the surface activation marker CD25 on CD8+ T cells in the presence of tumor target cells. Briefly, human MCSP-expressing MV-3 tumor target cells were harvested with Cell Dissociation Buffer, washed and resuspendend in DMEM containing 2% FCS and 1% GlutaMax. 30 000 cells per well were plated in a round-bottom 96-well plate and the respective antibody dilution was added at the indicated concentrations (Figure 8). The bispecific construct and the ent IgG controls were ed to the same molarity. Cynomolgus PBMC or cells, isolated from blood of two y animals, were added to obtain a final E:T ratio of 3:1. After incubation for 43 h at 37°C, 5% CO2, the cells were centrifuged at 350 x g for 5 min and washed twice with PBS, containing 0.1% BSA. Surface staining for CD8 (Miltenyi Biotech 80-601) and CD25 (BD #557138) was performed according to the er’s suggestions. Cells were washed twice with 150 µl/well PBS containing 0.1% BSA and fixed for 15 min at 4°C, using 100 µl/well fixation buffer (BD #554655). After centrifugation, the samples were resuspended in 200 l PBS with 0.1% BSA and analyzed using a FACS I machine (Software FACS Diva).

As depicted in Figure 8, the bispecific construct induces concentration-dependent up-regulation of CD25 on CD8+ T cells only in the presence of target cells. The anti cyno CD3 IgG (clone FN- 18) is also able to induce up-regulation of CD25 on CD8+ T cells, without being crosslinked to tumor target cells (see data obtained with cyno Nestor). There is no hyperactivation of cyno T cells with the maximal concentration of the bispecific construct (in the absence of target cells).

In another experiment, the CD3-MCSP “2+1 IgG Crossfab (N-terminal), linked light chain” (see SEQ ID NOs 1, 4, 5 and 85) was ed to the CD3-MCSP “2+1 IgG Crossfab (N-terminal)” (see SEQ ID NOs 1, 3, 4 and 5) for its potential to up-regulate the early activation marker CD69 or the late activation marker CD25 on CD8+ T cells in the ce of tumor target cells. Primary human PBMCs (isolated as described above) were incubated with the indicated concentrations of bispecific constructs for at least 22 h in the ce or absence of MCSP-positive Colo38 target cells. Briefly, 0.3 million primary human PBMCs were plated per well of a flat-bottom 96-well plate, containing the MCSP-positive target cells (or medium). The final effector to target cell (E:T) ratio was 10:1. The cells were incubated with the indicated concentration of the bispecific constructs and controls for the indicated incubation times at 37°C, 5% CO2. The effector cells were stained for CD8, and CD69 or CD25 and analyzed by FACS CantoII.

Figure 19 shows the result of this experiment. There were no significant differences detected for CD69 (A) or CD25 up-regulation (B) between the two 2+1 IgG Crossfab molecules (with or without the linked light chain).

In yet another ment, the CD3-MCSP “2+1 IgG Crossfab (N-terminal)” (see SEQ ID NOs 1, 3, 4, 5) and “1+1 IgG Crossfab minal)” (see SEQ ID NOs 1, 3, 4, 86) constructs were compared to a bispecific CD3-MCSP IgG-like construct having one antigen binding arm replaced by a Crossfab fragment (“1+1 CrossMab”; see SEQ ID NOs 4, 87, 88, 89) for their potential to up-regulate CD69 or CD25 on CD4+ or CD8+ T cells in the ce of tumor target cells. The assay was performed as described above, in the presence of absence of human MCSP expressing MV-3 tumor cells, with an incubation time of 24 h.

As shown in Figure 21, the “1+1 IgG Crossfab (N-terminal)” and “2+1 IgG Crossfab (N- terminal)” ucts induced more pronounced upregulation of activation markers than the “1+1 CrossMab” molecule.

Example 4 Re-directed T cell cytotoxicity ed by cross-linked bispecific constructs targeting CD3 on T cells and MCSP on tumor cells (LDH release assay) Bispecific constructs were analyzed for their potential to induce T cell-mediated apoptosis in tumor target cells upon crosslinkage of the construct via binding of the antigen binding moieties to their respective target antigens on cells.

In one experiment purified “2+1 IgG Crossfab (N-terminal)” construct (SEQ ID NOs 1, 3, 4, 5), targeting human CD3 and human MCSP, and the ponding “(scFv)2” molecule were compared. Briefly, huMCSP-expressing MDA-MB-435 human melanoma target cells were harvested with Cell Dissociation Buffer, washed and resuspendend in AIM-V medium (Invitrogen # 091). 30 000 cells per well were plated in a round-bottom 96-well plate and the respective dilution of the construct was added at the indicated concentration. All constructs and controls were ed to the same molarity. Human pan T effector cells were added to obtain a final E:T ratio of 5:1. As a ve control for the activation of human pan T cells, 1 µg/ml PHA-M (Sigma #L8902) was used. For normalization, maximal lysis of the target cells (= 100%) was determined by incubation of the target cells with a final concentration of 1% Triton X-100. Minimal lysis (= 0%) refers to target cells co-incubated with effector cells, but t any construct or antibody. After an ght incubation of 20 h at 37°C, 5% CO2, LDH release of apoptotic/necrotic target cells into the supernatant was measured with the LDH detection kit (Roche Applied Science, #11 644 793 001), according to the manufacturer’s ctions.

As depicted in Figure 9, the “2+1 IgG Crossfab (N-terminal)” construct induces apoptosis in target cells comparable to the )2” molecule.

In a further experiment the purified “2+1 IgG Crossfab (N-terminal)” (SEQ ID NOs 1, 3, 4, 5), the “1+1 IgG Crossfab (N-terminal)” (SEQ ID NOs 1, 2, 3, 4) and the “(scFv)2” molecule were analyzed for their potential to induce T cell-mediated apoptosis in tumor target cells upon inkage of the construct via binding of both target antigens, CD3 and MCSP, on cells. -expressing MDA-MB-435 human melanoma cells were used as target cells, the E:T ratio was 5:1, and the tion time 20 h. The s are shown in Figure 10. The “2+1 IgG Crossfab (N-terminal)” construct induces apoptosis in target cells comparably to the “(scFv)2” molecule. The comparison of the mono- and nt “IgG Crossfab (N-terminal)” formats shows that the bivalent one is more potent in this assay.

In yet another experiment, purified “2+1 IgG Crossfab (N-terminal)” (SEQ ID NOs 4, 5, 6, 7) targeting cynomolgus CD3 and human MCSP, and the corresponding “(scFv)2“ construct were compared, using MCSP-expressing human melanoma cell line (MV-3) as target cells. y, MV-3 cells were harvested with Cell iation Buffer, washed and resuspendend in DMEM containing 2% FCS and 1% GlutaMax. 30 000 cells per well were plated in a round-bottom 96- well plate and the tive dilution of construct or reference IgG was added at the concentrations ted. The bispecific construct and the different IgG controls were adjusted to the same molarity. Cynomolgus PBMC or cells, isolated from blood of healthy cynomolgus, were added to obtain a final E:T ratio of 10:1. After incubation for 26 h at 37°C, % CO2, LDH release of apoptotic/necrotic target cells into the atant was measured with the LDH detection kit (Roche Applied Science, #11 644 793 001), according to the manufacturer’s instructions.

As depicted in Figure 11, the “2+1 IgG Crossfab (N-terminal)” construct is more potent in terms of EC50 than the “(scFv)2“ molecule.

In another set of experiments, the CD3-MCSP “2+1 IgG Crossfab (N-terminal), linked light chain” (see SEQ ID NOs 1, 4, 5 and 85) was compared to the CD3-MCSP “2+1 IgG Crossfab (N-terminal)” (see SEQ ID NOs 1, 3, 4 and 5). Briefly, target cells (human Colo-38, human MV- 3 or WM266-4 melanoma cells) were harvested with Cell Dissociation Buffer on the day of the assay (or with trypsin one day before the assay was started), washed and resuspended in the appropriate cell culture medium 640, including 2% FCS and 1% Glutamax). 20 000 - 30 000 cells per well were plated in a flat-bottom 96-well plate and the respective antibody dilution was added as indicated (triplicates). PBMCs as effector cells were added to obtain a final effector-to-target cell (E:T) ratio of 10:1. All constructs and controls were adjusted to the same molarity, incubation time was 22 h. Detection of LDH release and normalization was done as described above.

Figure 15 to 18 show the result of four assays performed with MV-3 melanoma cells (Figure 15), Colo-38 cells (Figure 16 and 17) or WM266-4 cells (Figure 18). As shown in Figure 15, the construct with the linked light chain was less potent compared to the one without the linked light chain in the assay with MV-3 cells as target cells. As shown in Figure 16 and 17, the construct with the linked light chain was more potent compared to the one without the linked light chain in the assays with high MCSP expressing 8 cells as target cells. Finally, as shown in Figure 18, there was no icant difference between the two constructs when high MCSP-expressing WM266-4 cells were used as target cells.

In another experiment, two CEA-targeting “2+1 IgG Crossfab (N-terminal), inverted” constructs were compared, wherein in the Crossfab fragment either the V regions , see SEQ ID NOs 3, 8, 9, 10) or the C regions 1, see SEQ ID NOs 9, 10, 87, 95) were exchanged. The assay was performed as bed above, using human PBMCs as or cells and human CEA-expressing target cells. Target cells (MKN-45 or LS-174T tumor cells) were harvested with trypsin-EDTA (LuBiosciences -096), washed and resuspendend in RPMI1640 (Invitrogen #42404042), including 1% Glutamax (LuBiosciences #35050087) and 2% FCS. 30 000 cells per well were plated in a round-bottom 96-well plate and the bispecific constructs were added at the indicated trations. All constructs and controls were adjusted to the same molarity. Human PBMC effector cells were added to obtain a final E:T ratio of 10:1, incubation time was 28 h. EC50 values were calculated using the GraphPad Prism 5 software.

As shown in Figure 22, the construct with the CL/CH1 exchange shows slightly better activity on both target cell lines than the construct with the VL/VH exchange. Calculated EC50 values were 115 and 243 pM on MKN-45 cells, and 673 and 955 pM on LS-174T cells, for the CL/CH1-exchange construct and the VL/VH-exchange construct, respectively. rly, two MCSP-targeting “2+1 IgG Crossfab (N-terminal)” constructs were compared, wherein in the Crossfab fragment either the V regions (VL/VH, see SEQ ID NOs 3, 91, 92, 93) or the C regions (CL/CH1, see SEQ ID NOs 87, 91, 93, 94) were exchanged. The assay was performed as described above, using human PBMCs as effector cells and human MCSP- expressing target cells. Target cells -4) were harvested with Cell iation Buffer (LuBiosciences #13151014), washed and resuspendend in RPMI1640 (Invitrogen #42404042), including 1% Glutamax (LuBiosciences #35050087) and 2% FCS. 30 000 cells per well were plated in a round-bottom 96-well plate and the constructs were added at the indicated concentrations. All constructs and controls were adjusted to the same molarity. Human PBMC effector cells were added to obtain a final E:T ratio of 10:1, tion time was 26 h. EC50 values were calculated using the GraphPad Prism 5 software.

As depicted in Figure 23, the two constructs show comparable activity, the construct with the CL/CH1 exchange having a slightly lower EC50 value (12.9 pM for the -exchange construct, compared to 16.8 pM for the VL/VH-exchange construct).

Figure 24 shows the result of a similar assay, performed with human MCSP-expressing MV-3 target cells. Again, both constructs show comparable ty, the construct with the CL/CH1 exchange having a slightly lower EC50 value (approximately 11.7 pM for the CL/CH1-exchange construct, compared to approximately 82.2 pM for the VL/VH-exchange construct). Exact EC50 values could not be calculated, since the killing curves did not reach a plateau at high concentrations of the compounds.

In a further experiment, the SP “2+1 IgG Crossfab (N-terminal)” (see SEQ ID NOs 1, 3, 4, 5) and “1+1 IgG Crossfab (N-terminal)” (see SEQ ID NOs 1, 3, 4, 86) constructs were compared to the SP “1+1 CrossMab” (see SEQ ID NOs 4, 87, 88, 89). The assay was performed as bed above, using human PBMCs as effector cells and WM266-4 or MV-3 target cells (E:T ratio = 10:1) and an incubation time of 21 h.

As shown in Figure 25, the “2+1 IgG Crossfab (N-terminal)” construct is the most potent molecule in this assay, followed by the “1+1 IgG Crossfab minal)” and the “1+1 ab”. This ranking is even more pronounced with MV-3 cells, expressing medium levels of MCSP, compared to high MCSP sing WM266-4 cells. The calculated EC50 values on MV-3 cells were 9.2, 40.9 and 88.4 pM, on WM266-4 cells 33.1, 28.4 and 53.9 pM, for the “2+1 IgG Crossfab (N-terminal)”, the “1+1 IgG Crossfab (N-terminal)” and the “1+1 CrossMab”, respectively.

Example 5 g of bispecific constructs to the respective target antigen on cells Binding of the different bispecific constructs to CD3 on Jurkat (ATCC 52) cells, and the respective tumor antigen MCSP on WM266-4 cells or CEA on LS174-T cells, was determined by FACS. Briefly, cells were harvested, counted and checked for viability. 0.15 – 0.2 million cells per well were plated in a round-bottom 96-well plate and incubated with the indicated concentration of the bispecific constructs and controls for 30 min at 4°C. For a better comparison, the constructs were normalized to same molarity. Cells were washed with PBS containing 0.1% BSA once. After incubation with a FITC-or PE-conjugated secondary antibody for 30 min at 4°C, bound constructs were detected using a FACSCantoII (Software FACS Diva). A FITC- or PE- conjugated AffiniPure F(ab’)2 Fragment goat anti-human IgG Fcγ nt Specific (Jackson Immuno Research Lab # 109098 / working solution 1:20, or #109170 / working solution 1:80, respectively) was used. Unless otherwise indicated, cells were fixed with 100 µl/well fixation buffer (BD 5) for 15 min at 4°C in the dark, centrifuged for 6 min at 400 x g and kept in 200 µl/well PBS containing 0.1% BSA until analysis. EC50 values were calculated using the GraphPad Prism 5 software.

Figure 26 shows the binding of CD3/CEA “2+1 IgG Crossfab (N-terminal), inverted” bispecific constructs with either a VL/VH (see SEQ ID NOs 3, 8, 9, 10) or a CL/CH1 exchange (see SEQ ID NOs 9, 10, 87, 95) in the ab fragment to human CD3, expressed by Jurkat cells, or to human CEA, expressed by LS-174T cells. As a control, the equivalent maximum concentration of the corresponding IgGs and the background staining due to the labeled 2ndary antibody (goat anti-human FITC-conjugated AffiniPure F(ab’)2 Fragment, Fcγ Fragment-specific, Jackson Immuno Research Lab # 6-098) were ed as well. Both constructs show good binding to human CEA, as well as to human CD3 on cells. The calculated EC50 values were 4.6 and 3.9 nM (CD3), and 9.3 and 6.7 nM (CEA) for the “2+1 IgG Crossfab (N-terminal), inverted )” and the “2+1 IgG Crossfab (N-terminal), inverted (CL/CH1)” constructs, respectively.

Figure 27 shows the binding of CD3/MCSP “2+1 IgG Crossfab (N-terminal)” (see SEQ ID NOs 1, 3, 4, 5) and “2+1 IgG Crossfab (N-terminal), inverted” (see SEQ ID NOs 4, 87, 89, 90) ucts to human CD3, expressed by Jurkat cells, or to human MCSP, expressed by WM266- 4 cells. While binding of both construct to MCSP on cells was ably good, the binding of the “inverted” construct to CD3 was reduced compared to the other construct. The calculated EC50 values were 6.1 and 1.66 nM (CD3), and 0.57 and 0.95 nM (MCSP) for the “2+1 IgG Crossfab (N-terminal), inverted” and the “2+1 IgG Crossfab (N-terminal)” constructs, respectively. * * * gh the foregoing invention has been described in some detail by way of illustration and e for purposes of clarity of understanding, the descriptions and examples should not be construed as ng the scope of the invention. The sures of all patent and ific literature cited herein are expressly incorporated in their entirety by reference.

Claims

1. A bispecific n binding molecule, comprising a first Fab fragment which specifically binds to a first n, a second Fab fragment which specifically binds to a second antigen, and 5 an Fc domain composed of a first and a second subunit capable of stable ation; wherein a) the bispecific antigen binding molecule provides monovalent binding to the first and/or the second antigen, b) (i) the first Fab fragment is fused at its C-terminus to the inus of the second Fab fragment, which is in turn fused at its C-terminus to the N-terminus of the first Fc domain 10 subunit, (ii) the second Fab fragment is fused at its C-terminus to the inus of the first Fab fragment, which is in turn fused at its C-terminus to the N-terminus of the first Fc domain subunit, or (iii) the second Fab fragment is fused at its C-terminus to the N- us of the first Fc domain subunit, which is in turn fused at its C-terminus to the N- terminus of the first Fab fragment , 15 c) in the first and/or the second Fab fragment one of the following replacements is made: (i) the variable domains VL and VH are replaced by each other, or (ii) the constant domains CL and CH1 are replaced by each other, provided that not the same replacement is made in the first and the second Fab fragment, 20 d) the bispecific antigen binding molecule does not comprise a single chain Fab fragment.

2. The bispecific antigen g molecule of claim 1, wherein the ement is made in the first Fab fragment.

3. The bispecific antigen binding molecule of claim 1 or 2, wherein the replacement is a replacement of the variable domains VL and VH by each other. 25

4. The bispecific antigen binding molecule of claim 1 or 2, n the replacement is a replacement of the constant domains CL and CH1 by each other.

5. The bispecific antigen binding molecule of any one of the preceding , essentially consisting of the first Fab fragment, the second Fab fragment, the Fc domain, and optionally one or more peptide linkers.

6. The bispecific antigen binding molecule of any one of claims 1 to 4, comprising a third Fab fragment which specifically binds to the first or the second antigen.

7. The bispecific antigen g molecule of claim 6, wherein the third Fab fragment is fused to the second Fc domain subunit. 5

8. The bispecific n binding molecule of claim 6 or 7, wherein the third Fab fragment is fused at its C-terminus to the N-terminus of the second Fc domain subunit.

9. The bispecific antigen binding le of any one of claims 6 to 8, wherein the third Fab fragment specifically binds to the second antigen.

10. The bispecific antigen binding molecule of any one of the preceding claims, wherein the 10 same replacement is made in Fab fragments that specifically bind to the same antigen.

11. The bispecific n binding le of any one of the preceding claims, providing monovalent binding to the first antigen.

12. The bispecific antigen binding molecule of any one of the preceding claims, wherein a replacement is made only in the first Fab fragment. 15

13. The bispecific antigen binding molecule of any one of the ing claims, wherein the Fc domain comprises a modification promoting the association of the first and second Fc domain subunit, wherein (a) in the CH3 domain of the first subunit of the Fc domain an amino acid residue is ed with an amino acid e having a larger side chain volume, thereby generating a protuberance 20 within the CH3 domain of the first subunit which is positionable in a cavity within the CH3 domain of the second subunit, and in the CH3 domain of the second subunit of the Fc domain an amino acid residue is replaced with an amino acid residue having a smaller side chain , thereby generating a cavity within the CH3 domain of the second subunit within which the protuberance within the CH3 domain of the first subunit is positionable; or 25 (b) at the interface of the two Fc domain subunits one or more amino acid residues is/are replaced by charged amino acid residues so that homodimer formation s electrostatically unfavorable but heterodimerization electrostatically favorable.

14. The bispecific antigen binding molecule of any one of the preceding claims, wherein the Fc domain is an IgG Fc domain.

15. The bispecific antigen binding molecule of any one of the preceding claims, wherein the Fc domain is an IgG1 or IgG4 Fc domain.

16. The bispecific antigen binding le of any one of the preceding claims, wherein the Fc domain is human. 5

17. The bispecific antigen binding le of any one of the preceding claims, wherein (i) the Fc domain comprises one or more amino acid substitution that reduces the g affinity of the Fc domain to an Fc receptor and/or or function, wherein said amino acid substitution is at a on selected from the group of E233, L234, L235, N297, P331 and P329 (EU numbering); or (ii) the Fc domain is engineered to comprise an increased proportion of non-fucosylated 10 oligosaccharides, as compared to a non-engineered Fc domain..

18. An isolated polynucleotide encoding the bispecific antigen binding molecule of any one of claims 1 to 17.

19. An expression vector sing the ed polynucleotide of claim 18.

20. A host cell comprising the ed polynucleotide of claim 18 or the expression vector of 15 claim 19, wherein the host cell is not a human cell within a human.

21. A method for producing the bispecific antigen binding molecule of any one of claims 1 to 17, comprising the steps of a) culturing the host cell of claim 20 under conditions suitable for the expression of the bispecific antigen binding le and b) recovering the bispecific antigen binding molecule. 20

22. A pharmaceutical composition comprising the bispecific antigen binding molecule of any one of claims 1 to 17 and a pharmaceutically able carrier.

23. A bispecific antigen binding molecule as claimed in any one of claims 1 to 17, substantially as herein described with reference to any example thereof.

24. An isolated polypeptide as claimed in claim 18, substantially as herein described with 25 reference to any example thereof.

25. An expression vector as claimed in claim 19, substantially as herein described with reference to any example thereof.

26. A host cell as claimed in claim 20, substantially as herein described with nce to any e thereof.

27. A method as claimed in claim 21, ntially as herein described with reference to any example thereof.

28. A pharmaceutical composition as claims in claim 22, substantially as herein described with reference to any example thereof.