NZ709911B2 - Use of the phytocannabinoid cannabidiol (cbd) in combination with a standard anti-epileptic drug (saed) in the treatment of epilepsy - Google Patents

Use of the phytocannabinoid cannabidiol (cbd) in combination with a standard anti-epileptic drug (saed) in the treatment of epilepsy Download PDF

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NZ709911B2
NZ709911B2 NZ709911A NZ70991112A NZ709911B2 NZ 709911 B2 NZ709911 B2 NZ 709911B2 NZ 709911 A NZ709911 A NZ 709911A NZ 70991112 A NZ70991112 A NZ 70991112A NZ 709911 B2 NZ709911 B2 NZ 709911B2
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cbd
seizure
seizures
combination
epilepsy
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Gary Stephens
Benjamin Whalley
Claire Williams
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Gw Pharma Limited
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    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2300/00Mixtures or combinations of active ingredients, wherein at least one active ingredient is fully defined in groups A61K31/00 - A61K41/00
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00Medicinal preparations containing organic active ingredients
    • A61K31/045Hydroxy compounds, e.g. alcohols; Salts thereof, e.g. alcoholates
    • A61K31/05Phenols
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00Medicinal preparations containing organic active ingredients
    • A61K31/185Acids; Anhydrides, halides or salts thereof, e.g. sulfur acids, imidic, hydrazonic or hydroximic acids
    • A61K31/19Carboxylic acids, e.g. valproic acid
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00Medicinal preparations containing organic active ingredients
    • A61K31/33Heterocyclic compounds
    • A61K31/335Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin
    • A61K31/35Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin having six-membered rings with one oxygen as the only ring hetero atom
    • A61K31/352Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin having six-membered rings with one oxygen as the only ring hetero atom condensed with carbocyclic rings, e.g. methantheline 
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00Medicinal preparations containing organic active ingredients
    • A61K31/33Heterocyclic compounds
    • A61K31/395Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
    • A61K31/40Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having five-membered rings with one nitrogen as the only ring hetero atom, e.g. sulpiride, succinimide, tolmetin, buflomedil
    • A61K31/4015Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having five-membered rings with one nitrogen as the only ring hetero atom, e.g. sulpiride, succinimide, tolmetin, buflomedil having oxo groups directly attached to the heterocyclic ring, e.g. piracetam, ethosuximide
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00Medicinal preparations containing organic active ingredients
    • A61K31/33Heterocyclic compounds
    • A61K31/395Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
    • A61K31/495Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with two or more nitrogen atoms as the only ring heteroatoms, e.g. piperazine or tetrazines
    • A61K31/505Pyrimidines; Hydrogenated pyrimidines, e.g. trimethoprim
    • A61K31/513Pyrimidines; Hydrogenated pyrimidines, e.g. trimethoprim having oxo groups directly attached to the heterocyclic ring, e.g. cytosine
    • A61K31/515Barbituric acids; Derivatives thereof, e.g. sodium pentobarbital
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18Magnoliophyta (angiosperms)
    • A61K36/185Magnoliopsida (dicotyledons)
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K45/00Medicinal preparations containing active ingredients not provided for in groups A61K31/00 - A61K41/00
    • A61K45/06Mixtures of active ingredients without chemical characterisation, e.g. antiphlogistics and cardiaca
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00Drugs for disorders of the nervous system
    • A61P25/08Antiepileptics; Anticonvulsants

Abstract

Provided is a combination of cannabidiol (CBD) at a dose greater than 300mg/day and a standard anti-epileptic drug (SAED) which acts via sodium or calcium channels. Disclosed SAEDs include ethosuximide, valproate, and phenobarbital.

Description

USE OF THE PHYTOCANNABINOID CANNABIDIOL (CBD) IN COMBINATION WITH A STANDARD ANTI-EPILEPTIC DRUG (SAED) IN THE TREATMENT OF EPILEPSY This application is a divisional of New Zealand patent application no. 613307, dated 3 January 2012.
[0001] Generally described is the use of the phytocannabinoid cannabidiol (CBD) in combination with a standard anti-epileptic drug (SAED). Preferably the CBD is used in combination with a SAED with a mechanism of action which acts via sodium or calcium channels, more preferably one which: • modifies low-threshold or transient neuronal calcium currents, as exemplified by ethosuximide; or • reduces high-frequency neuronal firing and sodium-dependent action potentials and may additionally enhance GABA effects, as exemplified by valproate.
BACKGROUND
[0002] Epilepsy is a chronic neurological disorder presenting a wide spectrum of diseases that affect approximately 50 million people worldwide (Sander, 2003). Advances in the understanding of the body’s internal ‘endocannabinoid’ system has lead to the suggestion that cannabis-based medicines may have the potential to treat this disorder of hyperexcitability in the central nervous system (Mackie, 2006, Wingerchuk, 2004, Alger, 2006).
[0003] Cannabis has been ascribed both pro-convulsant (Brust et al., 1992) and anti- convulsant effects. Therefore, it remains to determine whether cannabinoids represent a yet to be unmasked therapeutic anticonvulsant or, conversely, a potential risk factor to recreational and medicinal users of cannabis (Ferdinand et al., 2005).
In 1975 Consroe et al. described the case of young man whose standard treatment (phenobarbitol and phenytoin), didn't control his seizures. When he began to smoke cannabis socially he had no seizures. However when he took only cannabis the seizures returned. They concluded that 'marihuana may possess an anti-convulsant effect in human epilepsy'.
A study by Ng (1990) involved a larger population of 308 epileptic patients who had been admitted to hospital after their first seizure. They were compared to a control population of 294 patients who had not had seizures, and it was found that using cannabis seemed to reduce the likelihood of having a seizure. However this study was criticized in an Institute of Medicine report (1999) which claimed it was 'weak', as 'the study did not include measures of health status prior to hospital admissions and differences in their health status might have influenced their drug use' rather than the other way round.
Three controlled trials have investigated the anti-epilepsy potential of cannabidiol. In each, cannabidiol was given in oral form to sufferers of generalised grand mal or focal seizures.
Cunha et al (1980) reported a study on 16 grand mal patients who were not doing well on conventional medication. They received their regular medication and either 200-300mg of cannabidiol or a placebo. Of the patients who received CBD, 3 showed complete improvement, 2 partial, 2 minor, while 1 remained unchanged. The only unwanted effect was mild sedation. Of the patients who received the placebo, 1 improved and 7 remained unchanged.
Ames (1986) reported a less successful study in which 12 epileptic patients were given 200-300mg of cannabidiol per day, in addition to standard antiepileptic drugs. There seemed to be no significant improvement in seizure frequency.
Trembly et al (1990) performed an open trial with a single patient who was given 900- 1200mg of cannabidiol a day for 10 months. Seizure frequency was markedly reduced in this single patient.
[0010] In addition to the disclosures suggesting CBD may be beneficial there is a report (Davis & Ramsey) of tetrahydrocannabinol (THC) being administered to 5 institutionalized children who were not responding to their standard treatment (phenobarbital and phenoytin). One became entirely free of seizures, one became almost completely free of seizures, and the other three did no worse than before.
[0011] In it is suggested that the cannabinoid Tetrahydrocannabivarin (THCV) may behave as anti epileptic, something confirmed by Thomas et al 2005.
In addition describes a THCV and CBD pharmaceutical formulation.
Such a formulation is suggested to be of use in many different types of diseases including epilepsy.
[0013] However, there are more than forty recognisable types of epileptic syndrome partly due to seizure susceptibility varying from patient to patient (McCormick and Contreras, 2001, Lutz, 2004) and a challenge is finding drugs effective against these differing types.
Neuronal activity is a prerequisite for proper brain function. However, disturbing the excitatory - inhibitory equilibrium of neuronal activity may induce epileptic seizures. These epileptic seizures can be grouped into two basic categories: a. Partial, and b. Generalised seizures.
Partial seizures originate in specific brain regions and remain localised – most commonly the temporal lobes (containing the hippocampus), whereas generalised seizures appear in the entire forebrain as a secondary generalisation of a partial seizure (McCormick and Contreras, 2001, Lutz, 2004). This concept of partial and generalised seizure classification did not become common practice until the International League Against Epilepsy published a classification scheme of epileptic seizures in 1969 (Merlis, 1970, Gastaut, 1970, Dreifuss et al., 1981).
The International League Against Epilepsy further classified partial seizures, separating them into simple and complex, depending on the presence or the impairment of a consciousness state (Dreifuss et al., 1981).
The league also categorized generalised seizures into numerous clinical seizure types, some examples of which are outlined below: Absence seizures occur frequently, having a sudden onset and interruption of ongoing activities. Additionally, speech is slowed or impeded with seizures lasting only a few seconds (Dreifuss et al., 1981).
Tonic-clonic seizures, often known as “grand mal”, are the most frequently encountered of the generalised seizures (Dreifuss et al., 1981). This generalised seizure type has two stages: tonic muscle contractions which then give way to a clonic stage of convulsive movements. The patient remains unconscious throughout the seizure and for a variable period of time afterwards.
Atonic seizures, known as “drop attacks”, are the result of sudden loss of muscle tone to either a specific muscle, muscle group or all muscles in the body (Dreifuss et al., 1981).
The onset of epileptic seizures can be life threatening with sufferers also experiencing long-term health implications (Lutz, 2004). These implications may take many forms: • mental health problems (e.g. prevention of normal glutamatergic synapse development in childhood); • cognitive deficits (e.g. diminishing ability of neuronal circuits in the hippocampus to learn and store memories); and • morphological changes (e.g. selective loss of neurons in the CA1 and CA3 regions of the hippocampus in patients presenting mesial temporal lobe epilepsy as a result of excitotoxicity) (Swann, 2004, Avoli et al., 2005)
[0021] It is noteworthy that epilepsy also greatly affects the lifestyle of the sufferer – potentially living in fear of consequential injury (e.g. head injury) resulting from a grand mal seizure or the inability to perform daily tasks or the inability to drive a car unless having had a lengthy seizure- free period (Fisher et al., 2000).
There are many different standard anti-epileptic drugs available at the present time including: acetozolamide, carbamazepine, clobazam, clonazepam, ethosuximide, eslicarbazepine acetate, gabapentin, lacosamide, lamotriquine, levetiracetam, oxcarbazepine, Phenobarbital, phenytoin, pregabalin, primidone, rufinamide, sodium valproate, tiagabine, topiramate, valproate, vigabatrin, and zonisamide.
[0023] The mode of action of some of these is understood and for others is unknown. Some modes of action are set out in Table 1 below: (Adapted from: Schachter SC. Treatment of seizures. In: Schachter SC, Schomer DL, eds. The comprehensive evaluation and treatment of epilepsy. San Diego, CA: Academic Press; 1997. p. 61-74) Table 1.
Sodium or calcium Antiepileptic drug Mechanism of action channel involvement Barbiturates: primidone Enhances GABAergic inhibition (Mysoline), phenobarbital Carbamazepine (Tegretol, Inhibits voltage-dependent sodium Sodium Tegretol-XR, Carbatrol) channels Modifies low-threshold or transient Calcium Ethosuximide (Zarontin) neuronal calcium currents Felbamate (Felbatol) Unknown Gabapentin (Neurontin) Unknown Inhibits voltage-dependent sodium Sodium channels, resulting in decreased Lamotrigine (Lamictal) release of the excitatory neurotransmitters glutamate and aspartate Blocks sodium-dependent action Sodium/Calcium Phenytoin (Dilantin, Phenytek) potentials; reduces neuronal calcium uptake Reduces high-frequency neuronal Sodium Valproate (Depakote, Depakote firing and sodium-dependent action ER, Depakene, valproic acid) potentials; enhances GABA effects Three well-established and extensively used in vivo models of epilepsy are: • pentylenetetrazole-induced (PTZ) model of generalised seizures (Obay et al., 2007, Rauca et al., 2004); • pilocarpine-induced model of temporal lobe (i.e. hippocampus) seizures (Pereira et al., 2007); and • penicillin-induced model of partial seizures (Bostanci and Bagirici, 2006).
These provide a range of seizure and epilepsy models, essential for therapeutic research in humans.
The application WO 02/064109 describes a pharmaceutical formulation where the cannabinoids THC and CBD are used. The application goes on to state that the propyl analogs of these cannabinoids may also be used in the formulation. Since this application was written it has been shown that THCV behaves in a very different manner to THC and therefore the assumption that the propyl analogs of cannabinoids behave in a similar manner to their pentyl counterparts is now not valid.
The application GB0911580.9 describes the use of THCV for the treatment of generalised seizures, also described is the use of the cannabinoid CBD in combination with the THCV.
It is an object of the present invention to identify novel drug combinations which will enhance or otherwise offer benefits in the use of SAED’s, and/or which at least provide the public with a useful choice. The use of a combination may allow for lower doses of SAED’s to be used then is conventional. [0028A] In this specification where reference has been made to patent specifications, other external documents, or other sources of information, this is generally for the purpose of providing a context for discussing the features of the invention. Unless specifically stated otherwise, reference to such external documents is not to be construed as an admission that such documents, or such sources of information, in any jurisdiction, are prior art, or form part of the common general knowledge in the art. [0028B] In the description in this specification reference may be made to subject matter that is not within the scope of the claims of the current application. That subject matter should be readily identifiable by a person skilled in the art and may assist in putting into practice the invention as defined in the claims of this application.
BRIEF SUMMARY OF THE DISCLOSURE [0028C] The present invention relates to the use of cannabidiol (CBD) at a dose of greater than 300 mg/day, in combination with valproate, in the manufacture of a medicament for use in reducing seizure frequency in generalized seizures.
Also described is cannabidiol, (CBD), at a dose of greater than 300mg/day, in combination with a standard anti-epileptic drug (SAED) which acts via sodium or calcium channels, for use in the treatment of epilepsy.
[0030] The SAED which acts via sodium or calcium channels may be exemplified by a drug which: • modifies low-threshold or transient neuronal calcium currents, such as, ethosuximide; or • reduces high-frequency neuronal firing and sodium-dependent action potentials (and may additionally enhance GABA effects), such as, valproate; In contrast, a SAED which (solely) enhances GABAergic inhibition (as opposed to acting via sodium or calcium channels), such as, phenobarbital, does not appear to provide benefits in combination with CBD, when tested in a pilocarpine model. Thus, the selective benefits of CBD with e.g. ethosuximide and valporate (SAED’s with defined and distinct mechanisms of actions involving calcium and sodium channels) could not be anticipated.
[0031] Also described is the use of cannabidiol (CBD), at a dose of greater than 300mg/day, in combination with a standard anti-epileptic drug (SAED) which acts via sodium or calcium channels, in the manufacture of a medicament for use in the treatment of epilepsy.
Also described is a method for the treatment of epilepsy, which comprises administering to a subject in need thereof cannabidiol (CBD), at a dose of greater than 300mg/day, in combination with a standard anti-epileptic drug (SAED) which acts via sodium or calcium channels.
Also described is a combination product comprising cannabidiol (CBD), at a dose of greater than 300mg/day, and a standard anti-epileptic drug (SAED) which acts via sodium or calcium channels.
[0034] The respective drugs may be packaged separately with instructions to be taken in combination or may be formulated as a single use product.
Preferably the standard anti-epileptic drug acting via sodium or calcium channels is taken from the group consisting of: ethosuximide and valproate.
Preferably the type of epilepsy to be treated is a generalised seizure or a temporal lobe seizure.
The combination may prove beneficial in one or more of the following: a. reducing the incidence of tonic-clonic seizures; b. increasing the amount of time a patient is seizure free; c. increasing the latency to onset of seizure; d. decreasing the overall duration of the seizure; and e. reducing the severity and mortality of the seizures.
Thus, the combinations are particularly well suited in the treatment of conditions generally considered refractory to existing medication. The combinations would also appear to allow for the use of lower doses of the SAED’s than would be used were the SAED to be used alone.
In one embodiment the CBD is used with one or more therapeutically effective other phytocannabinoid(s).
Preferably the one or more therapeutically effective other phytocannabinoid is THCV and / or CBDV.
In one embodiment the CBD is in an isolated form.
In a further embodiment the CBD is in the form of a botanical drug substance.
BRIEF DESCRIPTION OF THE DRAWINGS By way of example only, a number of embodiments, including embodiments of the invention are described hereinafter with reference to the accompanying drawings, in which
[0043] Figure 1 A-C shows the effect of CBD at 100 mg/kg in combination with valproate on PTZ-induced seizures; Figure 2 A-C shows the effect of CBD and valproate on latency, duration and severity of PTZ-induced seizures; Figure 3 A-C shows the effect of CBD at 100 mg/kg and ethosuximide on PTZ-induced seizures; Figure 4 A-C shows the anti-convulsant effects of 100 mg/kg CBD in combination with valproate on the development of pilocarpine-induced seizures; and Figure 5 A-C shows the effect of 100 mg/kg CBD in combination with valproate on the development of pilocarpine-induced seizure and mortality incidence.
[0048] Legend to Figure 1: A: % mortality with (black bars) and without 100 mg/kg CBD (white bars). B: % seizure free with (black bars) and without 100 mg/kg CBD (white bars). C: % of animals that developed the most severe (tonic-clonic) seizures with (black bars) and without 100 mg/kg CBD (white bars).
Legend to Figure 2: A: latency to seizure onset; B: duration of seizure activity of those animals that survived; C: median seizure severity.
Legend to Figure 3: A: latency to onset of seizures at different doses of ethosuximide without (black) or with (grey unfilled) 100 mg/kg CBD. B: Seizure severity. C: Percentage mortalities, key as in A.
Legend to Figure 4: Mean latency to onset (A), development of bilateral seizures (B) and tonic-clonic seizures (C).
Legend to Figure 5: A: Proportion (%) of animals in each dose group that exhibited fully developed tonic-clonic seizures. B: Proportion (%) of animals in each dose group that died. C: Proportion (%) of animals in each dose group that were seizure free.
DETAILED DESCRIPTION [0052A] The term “comprising” as used in this specification means “consisting at least in part of”. When interpreting each statement in this specification that includes the term “comprising”, features other than that or those prefaced by the term may also be present. Related terms such as “comprise” and “comprises” are to be interpreted in the same manner.
The examples below describe the use of isolated CBD in combination with standard anti-epileptic drugs (SAEDs) in two different models of epilepsy, namely the PTZ-induced seizure model and the pilocarpine-induced seizure model. The SAEDs used in these examples are ethosuximide, valproate and Phenobarbital (Pilocarpine model only). It is important to note that there are many different SAEDs available and the drugs chosen for these experiments provide a general overview of how the phytocannabinoid CBD is able to work in combination with different classes of drugs used in the treatment of epilepsy.
Example 1 The use of the phytocannabinoid CBD in combination with a standard anti-epileptic drug (SAED) in the PTZ-model of epilepsy Methodology: Animals: Male Wistar rats (P24-29; 75-110g) were used to assess the effects of the phytocannabinoid CBD in combination with SAEDs in the PTZ model of generalised seizures.
Animals were habituated to the test environment, cages, injection protocol and handling prior to experimentation. Animals were housed in a room at 21ºC on a 12 hour light: dark cycle (lights on 0900) in 50% humidity, with free access to food and water.
The human dose equivalent (HED) can be estimated using the following formula: HED = Animal dose (mg/kg) multiplied by Animal K Human K The K for a rat is 6 and the K for a human is 37.
Thus, for a human of approx 60Kg a 100mg/Kg dose in rat would equate to a human dose of about 1000mg. Human doses of greater than 300mg/day, through 400mg/day in 100mg intervals (namely through 500, 600, 700, 800, 900, 1000, 1100, 1200, 1300 and 1400mg) to as much as 2000mg/ day are envisaged based on dose escalating studies with CBD (Example 2).
Experimental setup: Five 6L Perspex tanks with lids were placed on a single bench with dividers between them. Closed-circuit television (CCTV) cameras were mounted onto the dividers to observe rat behaviour. Sony Topica CCD cameras (Bluecherry, USA) were linked via BNC cables to a low- noise PC via Brooktree digital capture cards (Bluecherry, USA). Zoneminder (http://www.zoneminder.com) software was used to monitor rats, start and end recordings and manage video files. In-house Linux scripts were used to encode video files into a suitable format for further offline analysis using The Observer (Noldus Technologies).
PTZ model:
[0057] A range of doses of PTZ (50-100mg/kg body weight) were used to determine the best dose for induction of seizures (data not shown). As a result, a dose of 80mg/kg injected intra- peritoneally (IP; stock solution 50mg/ml in 0.9% saline) were used to screen the CBD / SAEDs combinations.
Experimental Protocols:
[0058] On the day of testing, isolated CBD was administered via intra-peritoneal (i.p.) injection at a dose of 100 mg/kg alongside animals that were injected with a matched volume of the cannabinoid vehicle (2:1:17 ethanol:Cremophor: 0.9%w/v NaCl solution), which served as the negative control group. Animals were then observed for 1 hour, after which time they received an IP injection of 80mg/kg PTZ. Negative vehicle controls were performed in parallel with cannabinoid-dosed subjects. After receiving a dose of PTZ, animals were observed and videoed to determine the severity of seizure and latency to several seizure behaviour types (see in vivo analysis, below). Animals were filmed for half an hour after last sign of seizure, and then returned to their cage.
In vivo analysis:
[0059] Animals were observed during experimental procedures, but all analysis was performed offline on recorded video files using The Observer behavioural analysis software (Noldus, Netherlands). A seizure severity scoring system was used to determine the levels of seizure experienced by subjects (Pohl & Mares, 1987). All signs of seizure were detailed for all animals.
Table 1.1 Seizure severity scoring scale, adapted from Pohl & Mares, 1987.
Seizure score Behavioural expression Righting reflex 0 No changes to behaviour Preserved 0.5 Abnormal behaviour (sniffing, excessive washing, Preserved orientation) 1 Isolated myoclonic jerks Preserved 2 Atypical clonic seizure Preserved 3 Fully developed bilateral forelimb clonus Preserved 3.5 Forelimb clonus with tonic component and body twist Preserved 4 Tonic-clonic seizure with suppressed tonic phase Lost Fully developed tonic-clonic seizure Lost 6 Death Latency from injection of PTZ to specific indicators of seizure development: The latency (in seconds) from injection of PTZ to first myoclonic jerk (FMJ; score of 1), and to the animal attaining “forelimb clonus with tonic component and body twist” (score of 3.5) were recorded. FMJ is an indicator of the onset of seizure activity, whilst >90% of animals developed scores of 3.5, and so is a good marker of the development of more severe seizures.
Data are presented as the mean ± S.E.M. within an experimental group.
Maximum seizure severity:
[0061] This is given as the median value for each experimental group based on the scoring scale below.
Percentage mortality: The percentage of animals within an experimental group that died as a result of PTZ- induced seizures. Note that the majority of animals that developed tonic-clonic seizures (scores of 4 and 5) died as a result, and that a score of 6 (death) automatically denotes that the animal also experienced tonic-clonic seizures.
Seizure duration: The time (in seconds) from the first sign of seizure (typically FMJ) to either the last sign of seizure or, in the case of subjects that died, the time of death – separated into animals that survived and those that did not. This is given as the mean ± S.E.M. for each experimental group.
Statistics: For measures of latency and severity, one way analysis of variance (ANOVA) was performed on the test groups to detect overall combinational effects of CBD and SAEDs (p ≤0.05 considered significant).
Significant ANOVA results were followed by post hoc tests to test differences between vehicle and drug groups (Tukey’s test, p ≤0.05 considered significant).
Results: From Figure 1 it can be seen that the addition of CBD to the SAED valproate has an effect on reducing the percentage mortality and the incidence of tonic-clonic seizures. It is also shown that the combination of CBD and the higher dose of valproate is more effective at increasing the amount of time that the animal was seizure free.
Figure 2 demonstrates that the combination of CBD and valproate was able to increase the latency to onset of seizure at all dose ranges, in addition it decreased the overall duration of the seizure.
The data shown in Figure 3 demonstrates that the combination of CBD with the SAED ethosuximide was also effective at reducing the severity and mortality of the seizures. It also at the higher dose of ethosuximide was able to increase the latency to onset of the seizures.
Conclusion: The data demonstrated in this Example clearly shows that the combination of CBD with a SAED which has a mechanism of action involving sodium or calcium chanels is of value when treating generalised seizures.
Example 2 The use of the phytocannabinoid CBD in combination with a standard anti-epileptic drug (SAED) in the pilocarpine model of (temporal lobe) epilepsy Methodology: Isolated CBD was injected intra-peritoneally (IP) in the standard vehicle (1:1:18 ethanol:Cremophor:0.9% / NaCl) at doses of 50, 100 and 200mg/kg alongside animals that received vehicle alone at a matched volume. 15 minutes later methylscopolamine (1 mg/kg; to reduce peripheral muscarinic effects of pilocarpine) was administered followed, 45 minutes later by pilocarpine (380 mg/kg, IP) administration.
Results: Figure 4 demonstrates the anti-convulsant effects of a combination of CBD and valproate in the pilocarpine model of epilepsy. These data show that the combination of the CBD and valproate was able to increase the latency of onset of the seizure.
It can be seen from the data illustrated in Figure 5 that in addition to increasing the latency of onset of the seizure the combination of CBD and valproate was able to decrease mortality and the percentage of tonic-clonic seizures.
Table 2.1 below describes the data in more detail.
Table 2.1 Anti-convulsant effects of CBD and valproate in the pilocarpine model of epilepsy CBD Valproate CBD in Combination with Seizure Measure Effects Effects Valproate Effects ** # Mean number of episodes Mean time spent in episodes * Mean duration of episodes * Mean severity of episodes * ** Percentage ≥3 episodes # Percentage episode free ** Latency ** Duration Severity * Latency # Duration # Severity * ALL EPISODES EPISODE 2 EPISODE 1 Key: # = p<0.01; * = p<0.05; ** = p<0.01 The table above clearly shows some of the advantages of using a combination of the two compounds.
Table 2.2 below describes the effect of using the phytocannabinoid CBD in combination with yet a further SAED, phenobarbital, in the pilocarpine model of epilepsy.
Table 2.2 Effects of CBD and phenobarbital on the pilocarpine model of epilepsy CBD Phenobarbitol Seizure free (%) Onset latency (s) (mg/kg) (mg/kg) 0 0 0 750 100 0 0 500 0 10 25 800 100 10 25 750 0 20 55 900 100 20 55 930 0 40 75 1800 100 40 85 900 In contrast to the valproate combination data, this result demonstrate the selective nature of the combinations which is likely attributed to the different mechanisms of actions of these SAED’s.
Overall Conclusion: The data demonstrated in the above Examples shows that the combination of CBD with standard anti-epileptic drugs acting via sodium or calcium channels may be beneficial in the treatment of different types of epilepsy. This finding is of great significance to the many epilepsy sufferers whose condition is refractory to existing medication.

Claims (7)

1. The use of cannabidiol (CBD) at a dose of greater than 300 mg/day, in combination with valproate, in the manufacture of a medicament for use in reducing seizure frequency in generalized seizures.
2. The use of claim 1, wherein the type of generalized seizures are tonic-clonic seizures.
3. The use of claim 1 or claim 2, wherein the valproate is present in the medicament in a therapeutically effective amount.
4. The use of claim 1, wherein the type of generalized seizure to be treated is refractory to existing medication.
5. The use of claim 1, wherein the CBD is an isolated phytocannabinoid.
6. The use of claim 1, wherein the CBD is in the form of a botanical drug substance.
7. The use as claimed in any one of claims 1 to 6, substantially as herein described with reference to any example thereof.
NZ709911A 2011-01-04 2012-01-03 Use of the phytocannabinoid cannabidiol (cbd) in combination with a standard anti-epileptic drug (saed) in the treatment of epilepsy NZ709911B2 (en)

Applications Claiming Priority (3)

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GB1100043.7A GB2487712B (en) 2011-01-04 2011-01-04 Use of the phytocannabinoid cannabidiol (CBD) in combination with a standard anti-epileptic drug (SAED) in the treatment of epilepsy
GB1100043.7 2011-01-04
NZ613307A NZ613307B2 (en) 2011-01-04 2012-01-03 Use of the phytocannabinoid cannabidiol (cbd) in combination with a standard anti-epileptic drug (saed) in the treatment of epilepsy

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NZ709911A NZ709911A (en) 2016-12-23
NZ709911B2 true NZ709911B2 (en) 2017-03-24

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