NZ621517B - Method to remove microorganisms on plant material - Google Patents
Method to remove microorganisms on plant materialInfo
- Publication number
- NZ621517B NZ621517B NZ621517A NZ62151714A NZ621517B NZ 621517 B NZ621517 B NZ 621517B NZ 621517 A NZ621517 A NZ 621517A NZ 62151714 A NZ62151714 A NZ 62151714A NZ 621517 B NZ621517 B NZ 621517B
- Authority
- NZ
- New Zealand
- Prior art keywords
- pollen
- subjected
- ozone
- temperature
- actinidia
- Prior art date
Links
- 239000000463 material Substances 0.000 title description 2
- 244000005700 microbiome Species 0.000 title description 2
- 241000219068 Actinidia Species 0.000 claims abstract description 16
- CBENFWSGALASAD-UHFFFAOYSA-N ozone Chemical compound [O-][O+]=O CBENFWSGALASAD-UHFFFAOYSA-N 0.000 claims abstract description 15
- 230000001580 bacterial Effects 0.000 claims abstract description 11
- 241000196324 Embryophyta Species 0.000 claims abstract description 9
- 230000000844 anti-bacterial Effects 0.000 claims abstract description 6
- 230000000813 microbial Effects 0.000 claims abstract description 6
- 238000010438 heat treatment Methods 0.000 claims abstract description 4
- 201000010099 disease Diseases 0.000 claims 1
- 230000001717 pathogenic Effects 0.000 claims 1
- 244000052769 pathogens Species 0.000 claims 1
- 241000589516 Pseudomonas Species 0.000 abstract 1
- 239000000523 sample Substances 0.000 description 11
- 238000000034 method Methods 0.000 description 10
- 241000894006 Bacteria Species 0.000 description 3
- 230000000845 anti-microbial Effects 0.000 description 2
- 231100000676 disease causative agent Toxicity 0.000 description 2
- 150000002500 ions Chemical class 0.000 description 2
- 235000009434 Actinidia chinensis Nutrition 0.000 description 1
- 240000001101 Actinidia deliciosa Species 0.000 description 1
- 235000009436 Actinidia deliciosa Nutrition 0.000 description 1
- 241000193744 Bacillus amyloliquefaciens Species 0.000 description 1
- 241000589615 Pseudomonas syringae Species 0.000 description 1
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 230000003042 antagnostic Effects 0.000 description 1
- 239000005557 antagonist Substances 0.000 description 1
- 230000001364 causal effect Effects 0.000 description 1
- 238000001311 chemical methods and process Methods 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 150000001879 copper Chemical class 0.000 description 1
- 238000003379 elimination reaction Methods 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 210000000056 organs Anatomy 0.000 description 1
- ABLZXFCXXLZCGV-UHFFFAOYSA-N phosphorous acid Chemical compound OP(O)=O ABLZXFCXXLZCGV-UHFFFAOYSA-N 0.000 description 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 235000011149 sulphuric acid Nutrition 0.000 description 1
- 239000001117 sulphuric acid Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2/00—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
- A61L2/16—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor using chemical substances
- A61L2/20—Gaseous substances, e.g. vapours
- A61L2/202—Ozone
Abstract
method for eliminating the microbial load of the bacterial cells of Pseudomonas sryringae pv. actinidiae (PSA), said method involving an antibacterial treatment of Actinidia spp pollen in two successive phases: in the first, a plant sample is subjected to heating; in the second, the same sample is subjected to ozone. subjected to ozone.
Description
METHOD TO REMOVE MICROORGANISMS ON PLANT MATERIAL
DESCRIPTION
Field of technique.
The present ion concerns a method that can effectively eliminate the microbial
load of the bacterial cells of Pseudomonar yrmgae pv. m‘tz'm'dz'ae (Psa), causative agent of
bacterial canker of actinidia, an extremely virulent bacterium which attacks all s
and ies of Actinidia.
The methodology described is applicable to different plants of the Genus Acz‘z'm'dz'a to
prevent the serious consequences of the presence and spread of the bacterium;
specifically the method is applied to actinidia pollen potentially infected by Psa.
State of technique.
The state of current techniques adopted to defend against Pseudomonas syringae pv.
diae (Psa) involves the use of different gies that are applied individually
and/or in combination:
a) provide proper nourishment and stimulate the endogenous defences of Actinidia
plants istration of amino acids, potassium humates, phosphorous acid,
sulphuric acid (Mazzaglia et al.,, 2011); b) the use of copper salts to protect the
ent phenological phases of the actinidia (Fratarcangeli et al.,, 2010; Quattrucci et
al.,, 2010); c) t ion by the causal agent of bacterial canker of actinidia by
means of a natural antagonist (Bacillus amyloliquefaciens, strain D747), the only crop
protection product to date that has been registered in the European Community to
combat Psa, which can colonize and protect the organs of actinidia plants against this
bacterium (ht wwwintrachemit homecmelan =it§ion=3&news=15).
It is therefore the aim of this invention to identify a methodology that can be d
to plants of the genus Actinidia to eliminate the microbial load of the bacterial cells
of Psa, through the use of ozone, combined bactericidal activity and, subsequently,
ature
References:
AMZZAGLIA A, RENZI M, TARATUFOLO M. C, Romain A, BALESTRA G. M.
(207 7) Tecm'v/ye di campo e num'zz'one cam‘ro 2'1 camm de/ zéz'wz). L’Infomaz‘are Agrario 70, 64—
66; PRATARCANGEIJ L, ROSSEYTI A, MAZZAGLLA A, BALESTRA GM.
(2070). I! 7210/0 de/ mme 001/0 [0170 0/ 00mm batieric‘o de/ ,éz'wz'. INFORMATORE
O, 00/. 8; p. 52—55, ISSN‘ 9; QUATIRUCCI A, REZVZI M,
ROSSETTI A, RICCI L, TARATUFOLO C, MAZZAGLIA A, BALESTRA GM.
(2070) Carma barremo de/ kiwi yerde: Imam .vz‘mz‘egz'e dz‘ control/0. L’z’nfomaz‘ore agrario 76: 53-
58. INFORZVIATORE AGRARIO, 00/. 76;]3. 55-58, ISSN: 689.
Summary of the invention
In one aspect of the present invention there is provided a method for eliminating the
microbial load of the bacterial cells of 13500070772000; iyn'ngae pv. actz'm'dz'ae (PSA), said method
involving an antibacterial ent z'izz'dz'a spp pollen in two successive phases: in
the first, a plant sample is subjected to heating; in the second, the same sample is
subjected to ozone.
Detailed description of the invention — procedure used for a sample of
dia pollen ially infected by PSA.
A detailed description now follows to illustrate the characteristics and advantages of
this invention: the methodology involving the elimination of the microbial load of
the bacterial cells of Prettdomami yn'ngae pv. acz‘mz'dz'ae (Psa), the causative agent of
bacterial canker of actinidia, is applied to a plant of the genus Actinidia, specifically to
a sample of pollen potentially infected by Psa, which is given just by way of example
for all the many ent fields of application of the invention.
The process involves two .
Phase 1: is a non~chemical process in which Actinidia pollen is subjected to a specific
° C) for
temperature (42 a given period of time (30 minutes), during which time air is
blown onto the said sample. The fact that it is subjected to a specific temperature and
for a defined period of time means that an antimicrobial action is d on the
d sample of Actinidia pollen.
Phase 2: having already been subjected to phase 1, and with air continuing to be
blown on it, the sample is treated with an odourless and colourless substance
(ozone). A specific concentration of ozone is used (0.05 ppm) for a given period of
time (4 hours), and in specific environmental conditions: temperature of 5°C and a
relative air humidity of no more than 35%.
If used separately on the same plant sample, the antimicrobial effect of both phases
(1 and 2) is reduced.
The above methodology, in which the two phases (Phase 1 and Phase 2) are applied
in succession, increases the bactericidal action of temperature and ozone, taking due
account of other parameters (duration of treatment, temperature and 0/0 RH in the
presence of ozone), which are essential ingredients for the success of the ure.
As regards the ent of pollen samples, an ad hoc system could be developed, for
example, to treat lots of refrigerated young dia vines before they are planted in
the open field.
Brief description of Figure 1
Figure 1: The figure shows the application of the method that is the object of this
invention.
In the prototype, a sample of Actinidia pollen, potentially infected by PSA, is placed
inside a container equipped with an ozone generator (Reference 1), cooler
(Reference 2), fan (Reference 3), radiator (Reference 4), cold temperature probe
(Reference 5), hot temperature probe (Reference 6), protection flaps (Reference 7)
to stop the pollen sticking to the protruding parts inside the container, opening for
taking measurements with test tubes (Reference 8 ), tray to insert the pollen and
collect it after it has been d (Reference 9)
In the first phase of the procedure, air is blown inside the container onto the
Actinidia pollen, which is subjected to a ature of 42°C for 30 s. In
phase 2, the pollen is treated with ozone (03), at a specific concentration (0.05
ppm), for 4 hours, at a temperature of 5°C and maximum relative humidity of 35%.
Claims (7)
1. A method for ating the microbial load of the bacterial cells of Pseudomonar yrz’ngae pv. dc‘l‘ifiidide (PSA), said method involving an antibacterial treatment of Ac‘z‘z‘m'dz‘a spp pollen in two successive phases: in the first, a plant sample is subjected to heating; in the second, the same sample is subjected to ozone.
2. The method according to claim 1, n the antibacterial treatment is against Psez/domomy grin/gaze pv. 'm'dz'ae that is a bacterial plant pathogen capable of causing the plant disease called actinidia bacterial canker.
3. The method according to claim 1 or 2 in which pollen of the genus Ac‘tz'm'dm is subjected to heating at a temperature of 42° C for 30 minutes in the first phase.
4. The method according to any one of the preceding claims in which pollen of the genus 'm'dz'a is subjected to ozone at a concentration of about 0.05 ppm for about 4 hours, at a temperature of about 5°C and a maximum relative humidity of about 35% in the second phase.
5. The method according to any one of the preceding claims in which the temperature and ozone treatments are carried out in a confined environment where a number of ent parameters selected from the group consisting of % relative ty (RH) of the air, temperature, ozone concentration and air velocity can be controlled.
6. Acz‘z‘m'a’z‘a spp pollen when treated by the method according to any one of claims 1 to 5.
7. The method according to claim 1, substantially as herein bed with reference to the
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| ITRM24 | 2013-03-01 | ||
| IT000124A ITRM20130124A1 (en) | 2013-03-01 | 2013-03-01 | METHODOLOGY FOR MICROBIAL FILLING ON VEGETABLE MATERIAL (ACRONIMO MAMMAV) |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| NZ621517A NZ621517A (en) | 2015-05-29 |
| NZ621517B true NZ621517B (en) | 2015-09-01 |
Family
ID=
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