NZ620487B2 - Alkylamidothiazoles, cosmetic or dermatological preparations containing said alkylamidothiazoles, and use thereof to combat or prevent undesired pigmentation of the skin - Google Patents

Abstract

The disclosure relates to alkylamidothiazoles of the formula (I), wherein X, Y, R1 and R2 are as defined in the specification. The disclose also relates to cosmetic or dermatological preparations having an effective content of one or more alkylamidothiazoles, as well as the use thereof for the cosmetic or dermatological treatment and/or prophylaxis of undesired skin pigmentation. Example compounds include: N-(4-(2,4-Dihydroxyphenyl)thiazol-2-yl)isobutyramide N-(4-(2,4-dihydroxyphenyl)thiazol-2-yl)-4-(hydroxyl-methyl)cyclohexanecarboxamide N-(4-(2,4-dihydroxyphenyl)thiazol-2-yl)cyclohexane carboxamide N-(4-(2,4-dihydroxyphenyl)thiazol-2-yl)-2-(4-(hydroxymethyl)phenyl)acetamide 4-(2-isopropylamino)thiazol-4-yl)benzene-1,3-diol tic or dermatological treatment and/or prophylaxis of undesired skin pigmentation. Example compounds include: N-(4-(2,4-Dihydroxyphenyl)thiazol-2-yl)isobutyramide N-(4-(2,4-dihydroxyphenyl)thiazol-2-yl)-4-(hydroxyl-methyl)cyclohexanecarboxamide N-(4-(2,4-dihydroxyphenyl)thiazol-2-yl)cyclohexane carboxamide N-(4-(2,4-dihydroxyphenyl)thiazol-2-yl)-2-(4-(hydroxymethyl)phenyl)acetamide 4-(2-isopropylamino)thiazol-4-yl)benzene-1,3-diol

Description

/068362 Description ALKYLAMIDOTHIAZOLES, COSMETIC OR DERMATOLOGICAL PREPARATIONS CONTAINING SAID ALKYLAMIDOTHIAZOLES, AND USE F TO COMBAT OR PREVENT UNDESIRED PIGMENTATION OF THE SKIN The present invention relates to new alkylamidothiazoles, to cosmetic or dermatological preparations with a content of one or more such alkylamidothiazoles and to the use of such alkylamidothiazoles or preparations comprising such alkylamidothiazoles for combating or ting undesired pigmentation of the skin.

Melanocytes are responsible for the pigmenting of the skin; these are found in the lowest layer of the epidermis, the Stratum basale, alongside the basal cells as pigment—forming cells which, depending on the skin type, occur either individually or in rs of varying size.

Melanocytes contain, as characteristic cell organelles, somes, in which the melanin is formed.

Inter alia, upon stimulation by UV radiation, melanin is formed to a greater extent. This is transported via the living layers of the epidermis (keratinocytes) ultimately into the horny layer (corneocytes) and brings about a more or less pronounced brownish to brown-black skin color.

Melanin is formed as the end stage of an ive process in which tyrosine is converted, under the co~action of the enzyme tyrosinase, via several intermediates, to the brown to brown—black eumelanins (DHICA and DHI n), or, with the participation of sulfur—containing compounds, to the reddish pheomelanin. DHICA and DHI melanin are formed via the _ 2 _ common intermediates dopaquinone and dopachrome. The latter, sometimes with the participation of further enzymes, is converted either to indol—5,6— ecarboxylic acid or into 5,6—quinone, from which the two specified eumelanins are formed.

The formation of pheomelanin proceeds inter alia via the ediates dopaquinone and cysteinyldopa. The expression of the melanin—synthesizing enzymes is controlled by a specific transcription factor (microphthalmia—associated transcription factor, MITF).

Besides the described enzymatic processes of the melanin synthesis, further proteins are also of ance for the melanogenesis in the melanosomes. An important role here appears to be attributed to the so~called p—protein, although the exact function is still unclear.

As well as the above—described s of the melanin synthesis in the cytes, the transfer of the melanosomes, their stay in the mis and also their degradation and the degradation of the melanin are also of decisive importance for the pigmenting of the skin.

It was shown that the PAR-2 receptor is important for the transport of the melanosomes from the melanocytes into the keratinocytes (M. Seiberg et al., 2000, J.

Cell. Sci., ll3:3093~101).

In addition, size and shape of the somes have an influence on their light—scattering properties and thus the color appearance of the skin. For example, in black Africans there are more large spheroidal individual melanosomes, whereas in Caucasians, smaller melanosomes occurring in groups are to be found.

Problems with hyperpigmentation of the skin have a wide variety of causes and/or are accompanied phenomena of many ical processes, e.g. UV radiation (e.g.

PCT/E92012/068362 _ 3 _ freckles, Ephelides), Genetic disposition, incorrect pigmentation of the skin during wound healing or scarring (post—inflammatory hyperpigmentation) or skin aging (e.g. Lentigines seniles).

After matory reactions, the pigmentation system of the skin reacts with sometimes te reactions.

This can lead either to nflammatory igmentations or hypopigmentations. Post— inflammatory hypomelanoses often arise inter alia in conjunction with atopy, Lupus erythematosus and ,w-mm psoriasis. The different reaction forms of the pigmentation system of the human skin as a result of inflammatory phenomena are understood only very incompletely.

Problems with post—inflammatory hyperpigmentation often occur in darker skin types. ularly in d males, the problem of Pseudofollikulitis barbae is known, which is associated with cosmetically undesired incorrect pigmentation and/or leads to this. Forms of melasma, which occur in particular in women of Asiatic origin on the face and on the décolletage area, and also various forms of irregular pigmentation of the skin are also types of post—inflammatory hyperpigmentations. In addition, dark s around the eyes are also considered to be a form of post— inflammatory igmentations, the underlying inflammation in most cases proceeding without clinical manifestations.

In many cases, post—inflammatory incorrect pigmentation of this type is increased further by the action of sunlight (UV light) without resulting in a UV—induced inflammation (sunburn).

Active ingredients and preparations are known which counteract skin pigmentation. In practical use these _ 4 _ are essentially ations based on hydroquinone, although, on the one hand, these only exhibit their effect after application for several weeks, and, on the other hand, their excessively long application is unacceptable for toxicological reasons. Albert Kligman et al. has developed a led “triformula” which constitutes a combination of 0.1% tretinoin, 5.0% hydroquinone, 0.1% dexamethasone (A. Kligman, 1975, Arch. Dermatol., 111:40—48). r, this formulation too is highly disputed on account of possible irreversible changes in the pigmentation system of the skin.

In addition, skin—peeling s (chemical and mechanical “peels”) are used, although these often lead to inflammatory reactions and, on account of post— inflammatory hyperpigmentations which may subsequently arise, can even lead to greater pigmentation d of reduced pigmentation. All of these customary methods, which are also used for treating post~inflammatory hyperpigmentations, are characterized by distinct side effects.

Furthermore, s other substances are known for which a skin—lightening effectiveness is described.

Mention is to be made here inter alia of hexadecene— 1,16—dicarboxylic acid, kojic acid and derivatives, arbutin, ascorbic acid and derivatives, flavonoids, ellagic acid and derivatives, tranexamic acid and various resorcinol derivatives, such as e.g. 4—n— butylresorcinol, 4—n—hexylresorcinol and 4—(1— ethyl)benzene—l,3—diol.

J.M. Ready describes in a publication (Bioorganic & Medicinal try Letter 17 (2007) 6871-6875 the effect of inter alia substituted thiazole derivatives for the inhibition of mushroom tyrosinase.

The patent application from Shiseido () describes substituted thiazolamines and hydrothiazolamines for lightening skin.

The substances described in the aforementioned prior art show a moderate effectiveness and/or a poor cal ity.

Rings around the eyes can se be formed as a result of a pigmentation disorder, with them in addition also appearing as a reaction to general , such as e.g. too little sleep or simply as a result of overexerting the eyes. In younger people, the symptoms disappear again after an adequate nighttime rest, but, over prolonged periods, the condition can become chronic and very esome for those affected. There is also a lack of sufficiently ing active ingredients and treatment s to combat such skin phenomena.

It is an object of the present invention to overcome or ameliorate at least one of the disadvantages of the prior art, or to provide a useful alternative.

It was therefore an aim of a preferred embodiment of the invention. below to provide a remedy‘ for the disadvantageous prior art.

Any discussion of the prior art throughout the specification should in no way be considered as an admission that such prior art is widely known or forms part of common general knowledge in the field.

According to a first aspect, the present invention. provides alkylamidothiazole having any one of the following structures: HO OH N—(4—(2,4—dihydroxyphenyl)thiazol—2~yl)pivalamide HO OH N—(4w(2,4—dihydroxyphenyl)thiazol—z—yl)isobutyramide HO OH \ \mf/O\/\ N—(4—(2,4—dihydroxyphenyl)thiazol—2—yl)butyramide HO OH ._.—O \ °\/\/\/ N;(4—(2,4—dihydroxyphenyl)thiazol—Z—yl)heptanamide —5b- HO 0H N;(4—(2,4-dihydroxyphenyl)thiazol—Z—yl)hydroxyhexanamide | \>¥~H 0 0H N-(4—(2,4—dihydroxyphenyl)thiazol-Z-yl)hydroxypropanamide g \>_NH o o——— 2,4-dihydroxyphenyl)thiazol—Z—yl)methoxyacetami§e ; \>_~H 0 MHz N—(4-(2,4—dihydroxyphenyl)thiazol—Z—yl)propanimide HO OH \>’~<C’CHa S / N—(4—(2,4—dihydroxyphenyl)thiazol-2~yl)acetamide N- (4 -(2, ydroxyphenyl)thiazol- 2-WylI:\>_:H4-m: (hydroxymethyl)cyclohexanecarboxamide HO OH N~(4—(2,4—dihydroxyphenyl)thiazol—Z— yl)cyclohexanecarboxamide OH OH -Sd- N-(4—(2,4—dihydroxyphenyl)thiazol—Z—yl)—2—(4— hydroxymethyl)phenyl)acetamide.

According to a second. aspect, the present invention. provides cosmetic or dermatological preparations with a content of one or more alkylamidothiazoles as defined in the first .

According to a third aspect, the present ion provides use of one or more alkylamidothiazoles defined in the first aspect, or preparations containing one or more such alkylamidothiazoles in the manufacture of a ment for the cosmetic or dermatological treatment and/or" laxis of undesired. skin pigmentation.

Also described herein are alkylamidothiazoles of the general formula N 0 YflT/fl\w/ in which _ 6 _ R1, R2, X and Y can be different, partly identical or completely identical and, ndently of one another, can mean: R1 = ~C1~Cu—alkyl (linear and branched), ~C1~Cfl—alkenyl (linear and branched), -cycloalkyl, —C1-C3- cycloalkyl—alkylhydroxy, —C1—CM-alkylhydroxy (linear and branched), —C1—CM alkylamine (linear and ed), —alkylaryl (linear and branched), ~Cl—CN- alkylaryl~alkyl~hydroxy (linear and branched), ~C1uCMm alkylheteroaryl (linear and branched), ~C1—Cg—alkyle0~ alkyl (linear and branched), —C1—CM alky— morpholino, —C1—CM alky—piperidino, -C1-CM alky— piperazino, —C1—CM alky—piperazino—N—alkyl, R2 = H, -C1—CM-alkyl r and branched), —C1—CM— alkenyl r~ and. branched), —C1—C3—cycloalkyl, —C1~ Cu—hydroxyalkyl (linear and branched), —C1~Cu~alkylaryl (linear and branched), —C1-CN-alkylheteroaryl (linear and branched), X = —H, —C1—CM—alkyl (linear and branched), —C1*Cu— alkenyl (linear and, branched), —C1—C8-cycloalkyl, ~C1— Cu—aryl (optionally mono— or bstituted with —OH, -F, «Cl, ~Br, -1, ~OMe, —NH2, —CN), —C1—Cu—heteroaryl (optionally monOe or polysubstituted with —OH, —F, —Cl, -Br, —I, —OMe, —NHZ, —CN), —C1—CM—alkylaryl (linear and branched), —C1—C“—alkylheteroaryl (linear and branched), —aryl (optionally mono— or polysubstituted 3O with -OH, —F, -Cl, -Br, ~I, —0Me, -NH2, -CN), —phenyl, —2,4—dihydroxyphenyl, —2,3—dihydroxyphenyl, —2,4~ dimethoxyphenyl, w~2,3~-dimethoxyphenyl, Y = H, —C1—Cm~alkyl (linear and branched), —C1—Cm— alkenyl (linear and, branched), —C1—C3—cycloalkyl, —C1— Cu—aryl, heteroaryl, —alkylaryl (linear and branched), ~C1—CM—alkylheteroaryl (linear and branched), —aryl, -phenyl, —2,4—dihydroxyphenyl, -2,3- dihydroxyphenyl, —2,4~dimethoxyphenyl, —2,3—dimethoxyphenyl, ~COO—alkyl, —COO—alkenyl, ~COO-cycloalkyl, —COO—aryl, —COO~ heteroaryl; and X, Y can optionally also mean = condensed aromatic, where X and Y can form with one another aromatic or aliphatic homo- or heterocyclic ring systems with up to 11 ring—forming atoms, and where the number n can assume values from 5 to 8, and the respective ring s can in turn be substituted with up to n—l alkyl groups, hydroxyl groups, carboxyl groups, amino groups, nitrile functions, sulfur—containing substituents, ester groups and/or ether groups.

Said thiazoles can either be in the form of the free base or the salt: e.g. fluoride, chloride, bromide, iodide, sulfate, carbonate, ascorbate, acetate or phosphate. In particular in the form of halogen salts, such as e.g. chloride and bromide. rmore, there is an ageous ation of the present invention in a second aspect to provide cosmetic or dermatological preparations with an effective content of one or more entioned alkylamidothiazoles of the invention.

Also in a third aspect in accordance with the invention is the use of the aforementioned midothiazoles of the invention for the treatment and/or prophylaxis of undesired skin pigmentation.

Here, treatment and/or prophylaxis of undesired skin pigmentation can be both. in the cosmetic sphere and in the pharmaceutical sphere.

In this connection, the pharmaceutical (or ological) treatment is primarily understood for diseased skin conditions, whereas the cosmetic treatment and/or laxis of undesired skin pigmentation primarily relates to healthy skin.

Unless the context y requires ise, throughout the description and the claims, the words “comprise”, “comprising”, and the like are to be construed in an inclusive sense as opposed to an exclusive or exhaustive sense; that is to say, in the sense of “including, but not limited to”.

Advantageously, X is selected from the group of substituted phenyls, in which case the substituents (Z) can be selected from the group —H, —OH, —F, —Cl, -Br, —I, -OMe, —NH2, -CN, acetyl and can be identical or different.

"Ni, Particularly‘ advantageously; X is ed. from the group of phenyl groups substituted with one or more hydroxyl groups, in which case the substituent (Z) can be selected from the group - H, —OH, —F, —Cl, —Br, —I, ~0Me, ~NH2, —CN, acetyl, and preference is given to the following generic structure in which Y, R} and R? can have the properties defined above.

HO 2 Particularly ageous compounds are those in which ‘vy. _. 9 _ H0 2 Y = H R1 = 4—alkyl (linear and branched), —C1—C24—alkenyl (linear and ed), —C1—C3—cycloalkyl, —C1—C8— cycloalkyl—alkylhydroxy, —C1—C24 alkylhydroxy (linear and branched), -C1—C24 alkylamine (linear and branched), -C1—C24-alkylaryl (linear and branched), ”Cl-‘Cgr‘ alkylaryl—alkyl—hydroxy (linear and branched), 4— alkylheteroaryl (linear and branched), "C1-C24—alkyl—O— C1—C24—alkyl (linear and branched) , —C1—~C24—alky- morpholino, —Cl—C24 alky—piperidino, —C1——C24 alky— zino, —C1—C24 al ky—piperazino—N—al kyl R2 = H, —C1—C24—alkyl (linear and branched), Z = —H, -O§-I, —F, —Cl, —Br, ~I, —OMe, ~NH2, -CN, acetyl.

Particular preference is given to those compounds in which HO OH X n Y = 8 R1 = —C1—C24—alkyl (linear and ed), —C1—C24—alkenyl (linear and ed) , —C1—C3-cycloalkyl, ~C1—Cg— cycloalkyl—alkylhydroxy, —C1—C24-alkylhydroxy (linear _ 10 w and branched), —C1~CM mine (linear and branched), —alkylaryl (linear and branched), ~C1—Cu—alkyl— aryl—alkyl—hydroxy (linear and branched), —C1-C“— alkylheteroaryl (linear and branched), ~C1—CM-alkyl—O~ C1—Cu~alkyl (linear and branched), ~Cl-CM alkymorpholino , —C1—CN alky-piperidino, wC1—C24 alky~ piperazino, —C1—CM alky-piperazino—N—alkyl, R2=H.

The compounds HO OH N-(4-(2,4-dihydroxyphenyl)thiazol-2—y])pivalamide HO OH N~(4-(2,4-dihydroxyphenyl)thiazol—2—yl)isobutyramide WO 41526 \ N\>\T/l!\/\ N—(4~(2,4-dihydroxypheny1)thiazoI-2—yl)butyramide HO OH N-(4-(2,4-dihydroxyphenyl)thiazoiyl)heptanamide HO OH /C\/\/\/OH N-(4-(2,4-dihydroxyphenyl)thiazolyl)hydroxyhexauamide WO 41526 _ 12 _ l \H O OH N~(4—(2,4-dihydroxypheny])thiazol-2—y1)«3—hydroxypropanamide I >— oh N—(4—(2,4—di11yd1‘oxyphenyl)thiazol-2—yl)—2-methoxyacetamide 0 NH2 3-amino-N-(4-(2,4-dihydroxyphenyl)thiazol-Z-y1)propanamide ._ 13 _ HO OH \ \>—-’NH\c/CH3 S 0// N-(4~(2,4-dihydroxyphenyI)thiazol~2-yDacetamide ,:\>_NH 0H 2,4-dfi1ydroxypheny1)thiazolyI)(hydroxymethyi)cyc10hexanecarboxamide HO OH N-(4—(2,4—dihydroxyphenyl)thiazolyl)cyclohexanecarboxamide _ 14 - OH OH l \>,NH N-(4-(2,4-dihydroxyphenyl)111ia201—2-y1)(4-(hydroxymefl)yl)phenyl)acetamide are red according to the invention.

Surprisingly, it was able to be shown that the alkylamidothiazoles according to the invention have a higher galenical stability and/or increased effectiveness compared to the corresponding alkylaminothiazoles.

See Table l and Table 2.

Method description of the stability investigations: For the purposes of incorporation into the ations, the amines and amides were dissolved in butylene glycol ~ optionally with heating - and added to the emulsion before the first homogenization at ca. 65°C. All of the active ingredients were incorporated in a concentration of 0.1% in the experimental batches.

The emulsion was poured into 20 ml glass vials for the storage tests and stored under various standard conditions (room temperature, light and 40°C).

For the stability investigations, the stored s were analyzed after 14 days. _ 15 _ Analysis/recovery: The samples to be measured were extracted in a methanol/water mixture [70:30] and determined by means of HPLC—DAD. The determination was carried out by means of external standard calibration ence section].

The evaluation was carried out at 296 nm.

Instrument: HPLC: Agilent 1100 Column: Phenomenex Synergi , 50 x 2 mm i.d. [2.5 um] Solvent: nt acetonitrile/water with 0.1% phosphoric acid Flow rate: 0.3 ml/min Gradient: Time [min] H20 [%] MeCN {%] 0.1% H3904 0.0 90.0 10.0 .0 10.0 90.0 12.0 10.0 90.0 13.0 90.0 10.0 .0 90.0 10.0 Injection volume: 3 ul The raw materials used in each case served as reference.

WO 41526 2012/068362 i 16 i Table 1: Formula 1 — with N—(4—(2,4—dihydroxyphenyl)thiazolw 2~yl)isobutyramide Formula 2 — with 4—(2—isopropylamino)thiazol~4~ yl)benzene-l,3—diol Formula 3 — with 4—(2—(tert—butylamino)thiazol yl)benzene~1,3~diol Formula 4 — with N—(4—(2,4~dihydroxyphenyl)thiazol— 2—yl)pivalamide Formula 5 — with N—(4—(2,4—dihydroxyphenyl)thiazol- 2~yl)butyramide Formula 6 — with 4—(2—propy1amino)thiazol—4- yl)benzene—l,3—diol Formula 7 — with N—(4—(2,4—dihydroxyphenyl)thiazol— 2—yl)cyclohexanecarboxamide Formula 8 — with cyclohexylamino)thiazol—4- yl)benzene—l,3—diol Formula 9 — with N—(4—(2,4—dihydroxyphenyl)thiazol— 2—yl)heptanamide Formula 10 — with 4—(2—(hexylamino)thiazol—4— yl)benzene—l,3~diol _ 17 a Method descriEtion of the effectiveness igations: The iveness of the thiazoles was trated using an enzyme test in which conversion of L—DOPA to L—dopaquinone by a Inuman tyrosinase was measured. In this literature—known method (Winder, A.J. and Harris, H., New assays for the tyrosine hydroxylase and dopa oxidase activities of nase. Eur. J. Biochem. (1991), 198, 317—26), the reaction product L—dopaquinone is reacted with MBTH (3—methyl—2— benzothiazoline hydrazone) to give a pink—colored substance, the increase of which is measured over the time by absorption at 490 nm. Table 1 shows by way of example the effectiveness data for some of the claimed substances. It can be concluded from this that the substances according to the invention are extremely effective pigmentation—inhibiting substances. _ 18 _ Table 2: Inhibition of the tyrosinase activity by thiazoles Substance Inhibition Concentra (% of the —tion control) N—(4— (2 4Dihydroxyphenyl))thiazol— 2--yl)isobutyramide) "- —~—-yl)()benzene-l,3~diol ——-2— -—-Uyl heptanamide) _~"—-yl)(benzene 1, 3—diolyl)()benzene—1,3—diol —_g—-2—Vylpivalamide :—yl)butyramide2— ):)benzene—l,3—~diol 2—4yl) cyclohexanecarboxamide yl)(benzene— l, 3—diol N— (4— 2, ydroxyphenyl))thiazol— 10 ug/ml 2—yl)—4 ~(hydroxymethyl)~ cyclohexanecarboxamide (4—(Hydroxymethyl)phenyl)w 10 ug/ml amino)thiazol—4—yl)benzene~1,3~ diol N—(4—(2,4~Dihydroxyphenyl)thiazol— 10 ug/ml 2~yl)—2—(4—(hydroxymethyl)— phenyl)acetamide 2012/068362 _ 19 i S nthesis rocedures of alk lamidothiazoles selected b way of example: 2~Bromo—2’,4’—bismethox carbon lox aceto henone: Hour quam. Br2 PGO OPG THF CHCI W 70 % PG— COOCH3 Mitchell, David; Doecke, Christopher W.; Hay, Lynne A.; Koenig, Thomas M.; Wirth, David D. Tetrahedron Letters, 1995 A solution of 60 g (369 mmol) of 2,4-dihydroxy— acetophenone and 186 ml of triethylamine in 900 ml of tetrahydrofuran was cooled to 0°C, and 93 ml of methyl chloroformate in 400 ml of tetrahydrofuran was slowly added drOpwise. 11 white precipitate is formed. After stirring for 3 hours at room temperature, the reaction is complete (TLC control). The precipitate was filtered off with suction and washed with s amounts of tetrahydrofuran. The te was evaporated to dryness on a rotary evaporator, taken up in ethyl acetate, washed with 1N HCl and NaCl solution (sat.) and dried over ium sulfate, filtered from the magnesium sulfate, and the ethyl acetate was concentrated on a rotary evaporator. This gave 105 g of 2,4~bismethoxycarbonyloxyacetophenone. 1H NMR DQ: 8.05 (d, 1H), 7.38 (d, 18), 7.36 (s, 1H), 3.86 (d, 6H).

The product was used without further purification. 63 g (392 mmol) of bromine in 450 ml of chloroform were added dropwise to the solution of 105 g of 2,4—bismethoxycarbonyloxyacetophenone in chloroform (1000 ml) over the course of 3 h. The reaction was then stirred for a further 15 min at room temperature. The solvent was evaporated on a rotary evaporator. The WO 41526 _ 20 _ residue was stirred in ethyl acetate/n-hexane, and the resulting precipitate was filtered off with suction.

Recrystallization from ethyl e/n—hexane produced 100 g of 2—bromo—2’,4’—bismethoxycarbonyloxy- acetophenone. 1H NMR (DMSO—De): 8.11 (d, 1H), 7.42 (m, 2H), 4.87 (s, 2H), 3.87 (s, 3H), 3.85 (s, 3H) ppm; m.p.

N—(4—(2,4—Dih drox hen l)thiazol~2~ l) ide: “(W—K‘ :>1,JL\M O 3 ’fL\N tomene ’lL\ 11112111232321; fi M3 R30 095 0 1 peace sea + i m: 11 Br ,1 41511 iso {\N>__, 126 g (1.66 mmol) of thiourea were introduced into toluene (1000 ml), and 100 g (829 mmol) of pivaloyl chloride were added dropwise. The reaction solution was boiled under reflux for 3 hours, during which two phases formed. The upper phase was decanted off and cooled. The precipitated colorless needles were filtered off with suction and washed with cyclohexane and dried in vacuo. Yield: 64 g. 1H NMR {DMSO~D6): 10.27 (s, 18), 9.74 (s, 18), 9.40 (s, 1H), 1.19 (s, 9H) ppm. 107.7 g (310 mmol) of 2-bromo"2',4’-bismethoxycarbonyl- oxyacetophenone were boiled with 49.7 g (13.6 mmol) of N—pivaloylthiourea and 39.2 g (466 mmol) of NaHC03 in 1.2 1 of ethanol under reflux for 0.5 h. The reaction solution was cooled and admixed with 50.6 g (1.27 mol) of NaOH in 250 m1 of water. After stirring for 30 min at room temperature, the reaction solution was taken up with 300 ml of water and neutralized with 2N HCl. The _ 21 _ resulting precipitate was filtered off and recrystallized from ethanol/water. 80 g of thiazole were obtained. 1H NMR (DMSO—DG): 11.77 (bs, 1H), 11.02 (bs, 1H), 9.47 (bs, 2H), 7.65 (d, 18), 7.39 (s, 1H), 6.30 (s, 1H), 6.28 (d, 1H), 1.27 (s, 9H) ppm; m.p. 257—259°C.

N—(4—(2,4—Dih drox hen l)thiazol—2— l)isobut ramide: 0 S 0 S + k toluene i C! H?! RIf: ‘nyc 5:11 NH: {3°Cr GF‘G s Q 80 GM )L 1. meet} 801'? O + KN H “""7’ N * S < a H amho \ yflfiH e a 114 g (1.5 mol) of thiourea were introduced into e (800 ml), and 80 g (0.75 mol) of isobutyryl chloride were added dropwise. The reaction solution was boiled under reflux for 3 hours, during which two phases formed. The upper phase was decanted off and cooled. The precipitated white crystals were ed off with suction and washed with toluene and dried in vacuo. Yield: 62 g. 1H NMR (DMSO—Dd: 11.03 (bs, 1H), 9.66 (bs, 1H), 9.35 (bs, 1H), 2.72 (m, 1H), 1.03 (2, 6H) ppm. 89 g (260 mmol) of 2—bromo—2’,4’—bismethoxycarbonyl— oxyacetophenone were boiled under reflux with 37.5 g (260 mmol) of N—isobutyrylthiourea and 32 g (380 mmol) of NaHC03 in 1000 ml of ethanol for 0.5 h. The reaction solution was cooled and d with 41 g (0.93 mol) of NaOH in 250 m1 of water. After stirring for 30 min at room temperature, the reaction solution was taken up with 300 ml of water and adjusted to pH=3 with 2N HCl. _ 22 _ The resulting precipitate was filtered off and recrystallized from ethanol/water. 56 g of le were obtained. in NMR (DMSO—Ds): 12.16 (bs, 1H), 10.88 (bs, 1H), 9.47 (bs, 1H), 7.65 (m, 1H), 7.41 (s, 1H), 6.32 (m, 2H), 2.75 (m, 1H), 1.14 (d, 6H) ppm; m.p.

°C.

N~(4-(2,4—Dih drox hen l)thiazol—2— l)but ramide: O S 0 .3 ”/\\\’/JL\ 4' (Ji\ towene " """"'" 111‘ c: we N34. N iZfi'C NH: see ope H0 0% azmsco sea 0 .. mlAA tr & 2NKT£maHo ‘ >PNH G c 143 g (1.88 mol) of thiourea were introduced into e (1000 ml), and 100 g (0.93 mol) of n—butyryl chloride were added dropwise. The reaction solution was boiled under reflux for 3 hours, during which two phases formed. The upper' phase was decanted off and cooled. The precipitated slightly yellowish crystals were filtered off with suction and washed with toluene and dried in vacuo. Yield: 88 g. 1H NMR (DMSO—De): 11.03 (bs, 1H), 9.65 (bs, 1H), 9.33 (bs, 1H), 2.33 (t, 2H), 1.53 (m, 2H), 0.86 (t, 3H) ppm; m.p. 115-188°C 92 g (265 mmol) of 2—bromo—2',4'—bismethoxycarbonyl— oxyacetophenone were boiled under reflux with 38.75 g (265 mmol) of N-butyrylthiourea and 34 g (397 mmol) of NaHCO3 in 900 ml of ethanol for 0.5 h. The reaction solution was cooled and admixed with 37 g (0.93 mol) of NaOH in 300 ml of water. After stirring for 30 min at room temperature, the reaction solution was taken up with 300 ml of water and neutralized with 2N HCl. The resulting precipitate was filtered off and tallized from l/water. 67 g of thiazole _ 23 _ were obtained. 1H NMR (Dmsowe): 12.18 (bs, 10), 10.89 (be, 1H), 9.48 (bs, 1H), 7.65 (1 aronn H), 7.40 (s, 1H), 6.31 (2 arom. H), 2.43 (t, 2H), 1.64 (m, 2H), 0.91 (t, 38) ppm; m.p. 227—229°C.

N~(4—(2,4—Dih drox hen l)thiazol~2~ l)acetamide: PGO OPG s 0 JL HO OH 1. NaHCOa, EtOH 0 Br fl 2 N OHIEtOH/H o. a N j 2 \ N o 1 Sh 4.71 g (13.6 mmol) of 2-bromo—2’,4’—bismethoxycarbonyl— oxyacetophenone were boiled under reflux with 1.61 g (13.6 mmol) of N—acetylthiourea and 1.72 g (20.4 mmol) of NaHC03 in 45 ml of ethanol for 0.5 h. The reaction solution was cooled and admixed with 2.0 g (50 mmol) of NaOH in 20 ml of water. After ng for 20 min at 0°C, the reaction solution was taken up with 30 ml of water and neutralized with oncentrated HCl. The resulting precipitate was filtered off and recrystallized from ethanol/water. 2.73 g of product were obtained. in NMR (01480—06): 12.20 (b, 1H), 10.85 (s, 1H), 9.46 (s, 1H), 7.64 (m, 1H), 7.38 (s, 1H), 6.28 (m, 2H), 2.15 (s, 3H) ppm; m.p. 264—264°C.

N—(4—(2,4—Dih drox hen l)thiazol—2— l)—4—(h drox — methzl)czclohexanecarboxamide: O I. A2330. ne O cect.&rc 05. OR ______;____4_ “‘3 2. e130, 3003 Y9 Procedure ous to the literature. _ 24 _ BANYU Pharmaceutical Co. Ltd., EP2072519 A1, 2009 Yield: 96%. 1H NMR (DMSO-Ds): 12.03 (133, 1H), 3.85, 3.82 (2 X d, 2H), 2.50, 2.47 (2 x m, 1H), 2.00 (s, 3H), 0.95-1.90 (m, 9H) ppm o s S 1,somm7ec ,H\ OH + A N NH? HzN NH2 H \[ro 2. toluene, 120°C fir0 P60\I::]:n/A\WOPG Mu’JLW::1\J01Ks.Ncho aoH W‘I:::[;Elw2. NaoH/EIOH/H-0 \>FHN 95 g (0.47 mol) of 4~acetoxymethylcyclohexanecarboxylic acid were heated under reflux in 350 ml of thionyl chloride for 2 h. After removing the excess thionyl chloride in vacuo, the residue was taken up in 1 l of toluene, and 71 g (0.94 mol) of thiourea were added.

The reaction on was boiled under reflux for 3 hours and then filtered off while hot. After cooling the mother liquor, the resulting white crystals were filtered off with suction, washed with e and dried in vacuo. Yield: 59 g. 1H NMR (DMSO—DG): 11.03, .97 (2 x s, 1H), 9.64 (bs, 1H), 9.35 (bs, 18), 3.93, 3.82 (2 x d, 2H), 2.61, 2.42 (2 x m, 1H), 2.00 (s, 3H), 1.60 (m, 8H), 1.35, 0.94 (2 x m, 1H) ppm; 79 g (228 mmol) of o—2’,4’—bismethoxycarbonyl— oxyacetophenone were boiled under reflux for 0.5 h with 59 g (228 mmol) of ~-(4—acetoxymethylcyclo— hexylcarbonyl)thiourea and 29 g (340 mmol) of NaHC03 in 1000 ml of ethanol. The reaction solution was cooled and d with 73 g (1.8 mol) of NaOH in 300 m1 of water. After stirring for 30 min at room temperature, the reaction solution was taken up with 300 ml of water and adjusted to pH=3 with 2N HCl. The resulting _ 25 i itate was filtered off and recrystallized. from ethanol/water. 47 g of thiazol were obtained. 1H NMR (DMSO—Ds): 12.15, 12.10 (2 x s, 1H), 10.96 (2 x s, 1H), 9.47 (br, 2H), 7.64 (d, 1H), 7.39 (s, 1H), 6.29 (m, 2H), 4.40 (br, 1H), 3.32, 3.23 (2 x d, 2H), 2.65, 2.44 (2 X m, 1H), 1.90 (m, 1H), 1.78 (m, 2H), 1.50 (m, 58), 0.94 (m, 1H) ppm; m.p. 152—160°C.

N—(4—(2,4~Dih drox hen l)thiazol—2- l)c clohexane— carboxamide: o s 0 S + k toluene JL Cl HzN NH2 120°C ” NH2 PGO OPG S 0 HO OH + JL 1. Ncho,. ElOH HzN El —> N & ZNxmemwmo 1 §~fi 52 g (0.68 mol) of thiourea were introduced into toluene (500 ml), and 50 g (0.34 mol) of cyclohexanoyl chloride were added dropwise. The reaction solution was boiled under reflux for 3 hours, during which two phases formed. The upper phase was decanted off and cooled. The precipitated crystals were filtered off with suction, washed with toluene and recrystallized from methanol. Yield: 35 g. 1H NMR (DMSO—DG): 10.98 (bs, 1H), 9.65 (bs, 1H), 9.32 (bs, 1H), 2.49 (t, 1H), 1.75 (m, 4H), 1.61 (m, 1H), 1.18 (m, 5H) ppm. 92 g (265 mmol) of o—Z’,4’—bismethoxycarbonyl— oxyacetophenone were boiled under reflux for 0.5 h with 49.4 g (265 mmol) of N—cyclohexanoylthiourea and 34 g (397 mmol) of NaHCO3 in 900 ml of ethanol. The reaction on was cooled and d with 37 g (930 mmol) of NaOH in 300 ml of water. After stirring for 30 min at roonl temperature, the reaction solution was taken up with 300 ml of water and neutralized with 2N HCl. The ethanol was largely removed on a rotary ator. The precipitate formed was filtered off and recrystallized from ethanol/water. 70 g of thiazol were obtained. 1H NMR (DMSO—De): 12.14 (bs, 1H), 11.00 (bs, 1H), 9.48 (bs, 1H), 7.64 (1 arom. H), 7.39 (s, 1H), 6.30 (2 arom.

H), 2.49 (m, 1H), 1.84 (m, 2H), 1.76 (m, 2H), 1.65 (m, 1H), 1.42 (m, 28), 1.25 (m, 3H), ppm; m.p.: 262—266°C.

N-(4—(2,4—Dih drox hen l)thiazol—2~ l) ~2~(4~ (hydroxymethyl)Qhenyl)acetamide: 1 A020 pyridine ”OmOH CHCIs, 60°C OY 2 H0 100°C omOH Procedure analogous to the literature.

BANYU Pharmaceutical Co. Ltd., EP2072519 A1, 2009 Yield: 76%. 1H NMR (01150—06): 12.31 (bs, 1H), 7.26 (m, 4H), 5.05 (s, 2H), 3.57 (s, 2H), 2.05 (s, 3H) ppm 3 1. 300,, we 0§r’ OH 0 + JL N NW HZN NH2 ‘11! O 0 2. loluene. 120”C O 0 01-! P60 OPG H 1. Ncho3 ElOHH o + HEN a: \H/ 2 NaOHIE!OH/Ho s o CY “3,,“H O 3.7 g (18 mmol) of 4-acetoxymethylphenylacetic acid were heated under reflux in 40 ml of thionyl de for 2 h. After removing the excess l chloride in vacuo, the residue was taken up in 70 ml of toluene, WO 41526 _ 27 _ and 2.7 g (36 mmol) of thiourea were added. The reaction solution was boiled under reflux for 3 hours and then the solvent was removed in vacuo. Purification was by means of column chromatography with cyclohexane/ethyl acetate 1/1 on silica gel. Yield: 2.7 g. 1H NMR DG): 11.29 (bs, 1H), 9.55 (bs, 1H), 9.40 (bs, 1H), 7.30 (m, 4H), 5.04 (s, 2H), 3.71 (s, 2H), 2.05 (s, 3H) ppm; 3.5 g (10 mmol} of 2—bromo—2',4’—bismethoxycarbonyl— oxyacetophenone were boiled under reflux for 0.5 h with 2.7 g (10 mmol) of N—[2—(4—acetoxymethylphenyl)~ acetlethiourea and 1.3 g (15 mmol) of NaHC03 in 50 ml of ethanol. The reaction solution was cooled and admixed with 4.0 g (0.1 mol) of NaOH in 20 ml of water.

After stirring for 2 h at 60°C, the on solution was taken up in 100 ml of water and adjusted to pH=3 with 2N HCl. The resulting precipitate was filtered off and recrystallized from l/water. 1.3 g of thiazol were obtained. 1H NMR (DMSO-De): 12.44 (s, 1H), 10.80 (s, 1H), 9.48 (s, 1H), 7.66 (d, 1H), 7.41 (s, 1H), 7.29 (m, 4H), 6.32 (m, 2H), 5.13 (t, 1H), 4.47 (d, 2H), 3.77 (s, 2H) ppm; m.p. 254—256°C.

Cosmetic or dermatological preparations with a content of alkylamidothiazoles and their use for the treatment and/or prophylaxis of undesired skin pigmentation are likewise advantageous embodiments of the present ion.

It is particularly advantageous if such preparations comprise 0.000001 to 10% by weight, in particular 0.0001 to 3% by weight, very particularly 0.001 to 1% by weight, of one or more of the alkylamidothiazoles according to the invention, based on the total weight of the preparation. _ 28 _ Cosmetic and dermatological preparations according to the invention can be in various forms. Thus, they can be e.g. a solution, an anhydrous preparation, an emulsion or microemulsion of the water—in—oil (W/O) type or of the oil—in—water (O/W) type, a multiple emulsions, for example of the water—in—oil—in—water (W/O/W) type, a gel, a solid stick, a balm or else an aerosol. It is also advantageous according to the invention to administer the substances used according to the ion and/or their derivatives in encapsulated form, e.g. in collagen matrices and other customary encapsulation materials, e.g. as ose ulations, in gelatin or liposomally encapsulated.

It is also possible and advantageous within the context of the present invention to add the substances used according to the invention and/or their tives in aqueous s or surfactant preparations for cleaning the skin and the hair.

The lipid phase can advantageously be selected from the ing substance group: ~ l oils, mineral waxes — oils, such as triglycerides of capric acid or of caprylic acid, also natural oils such as e.g. castor oil; — fats, waxes and other natural and synthetic fatty bodies, preferably esters of fatty acids with alcohols of low carbon number, e.g. with 3O isopropanol, propylene glycol or glycerol, or esters of fatty alcohols with alkanoic acids of low carbon number or with fatty acids; ~ alkyl benzoates; * silicone oils such as dimethylpolysiloxanes, diethylpolysiloxanes, ylpolysiloxanes and mixtures thereof. . 29 i The oil phase of the emulsions, oleogels or hydrodispersions or lipodispersions within the context of the present invention is advantageously selected from the group of esters of saturated and/or unsaturated, branched and/or unbranched alkanecarboxylic acids of chain length frown 3 to 30 carbon atoms and ted and/or unsaturated, ed and/or unbranched alcohols of chain length from 3 to 30 carbon atoms, from the group of esters of aromatic carboxylic acids and saturated and/or unsaturated, branched and/or unbranched alcohols of chain length frmn 3 to 30 carbon atoms. Such ester oils can then advantageously be selected from the group pyl myristate, pyl palmitate, isopropyl stearate, isopropyl oleate, n~butyl stearate, l e, n—decyl oleate, yl stearate, isononyl stearate, yl isononanoate, 2-ethylhexyl palmitate, 2—ethylhexyl laurate, 2—hexyldecyl stearate, 2—octyldodecyl palmitate, oleyl oleate, oleyl erucate, erucyl oleate, erucyl erucate, and synthetic, semisynthetic and natural mixtures of such esters, e.g. jojoba oil.

In particular, mixtures of the aforementioned ts are used. In the case of alcohol ts, water may be a further constituent.

Emulsions according to the invention are advantageous and comprise e.g. said fats, oils, waxes and other 3O fatty bodies, and also water and an emulsifier, as is usually used for such a type of formulation.

Suitable propellants for preparations according to the invention which can be sprayed from aerosol containers are the customary known readily volatile, liquefied propellants, for example hydrocarbons (propane, butane, isobutene), which can be used on their own or in a _ 30 _ mixture with one another. Compressed air can also advantageously be used.

The examples below are intended to illustrate the present ion without ng it. Unless stated otherwise, all of the quantities, fractions and percentages given are percentages by weight, based on the weight and the total amount or on the total weight of the preparations.

Formulation examgles: INCI/Substance N—(4—(2,4—Dihydroxyphenyl)— thiazol—Z-yl)isobutyramide 4—(2—Isopropylamino)thiazol— 4—yl)benzene—l,3-diol 4-(2-tert- Butylamino)thiazol yl)benzene—1,3~diol N-(4-(2,4-Dihydroxyphenyl)- l-Z—yl)pivalamide N—(4~(2,4-Dihydroxyphenyl)- thiazol—Z—yl)butyramide 4 - (2— Propyl amino) thiazol—4 - II...- yl)benzene-l,3-diol 8.70 3.00 0.03 0.20 0.10 mmmmm 0.10 0.20 INCI/Substance Formula Formula Formula Formula 7 8 9 10 N—(4-(2,4—Dihydroxyphenyl)— thiazolyl)cyclohexane— carboxamide 4—(2—(Cyclohexylamino)— thiazolyl)benzene—l,3— diol N—(4—(2,4—Dihydroxyphenyl)- thiazol-Z-yl)heptanamide III- 4— (2— (Hexylamino) l --- yl)benzene—l,3“diol 2 . 00 0.70 0.00 0.00 0.20 0.00 -m-m_ 0.00 0.20

Claims (8)

1. midothiazole having any' one of the following structures : HO OH AF(4—(2,4—dihydroxyphenyl)thiazol—Z—yl)pivalamide HO OH N—(4—(2,4—dihydroxyphenyl)thiazol—Z—yl)isobutyramide HO OH \ ”\N/ \/\ N—(4~(2,4—dihydroxyphenyl)thiazol—Z-yl)butyramide HO OH \ NEW/QM N~(4~(2,4~dihydroxyphenyl)thiazol—Z—yl)heptanamide H0 OH N\ f \ N/"\/\/\/°H S .L 2,4—dihydroxyphenyl)thiazol—Z—yl)hydroxyhexanamide 01-! I \>_NH 0 0H N-(4—(2,4—dihydroxyphenyl)thiazol—Z—yl)hydroxypropanamide I "\>—~n ‘°‘ (he... N>(4-(2,4—dihydroxyphenyl)thiazol—Z—yl)methoxyacetamide \>—NH o NH2 N—(4w(2,4—dihydroxyphenyl)thiazol-Z-yl)propanimide HO OH \N\>,~H>/O// AF(4—(2,4—dihydr0xyphenyl)thiazol—Z—yl)acetamide N— (4— (2, ydroxyphenyl))thiazol2Wyl (hydroxymethyl)cyclohexanecarboxamide HO OH N~(4—(2,4—dihydroxyphenyl)thiazol—Z— lohexanecarboxamide OH OH I gen“\ N>(4—(2,4-dihydroxyphenyl)thiazol-z-yl)—2-(4- hydroxymethyl)phenyl)acetamide.

2. Alkylamidothiazole according to claim 1, wherein. in the form of halide, carbonate, ascorbate, sulfate, acetate or phosphate salt.

3. Cosmetic or dermatological preparations with a content of one or more alkylamidothiazoles as defined in claim 1 or claim ’5va 2.

4. Preparation according 133 claim 3, containing 0.000001 to 10% by weight of one or more of the midothiazoles defined in claim 1 or claim 2, based on the total weight of the preparation.

5. Preparation according to claim 3, ning in particular 0.0001 to 3% by weight of one or more of the alkylamidothiazoles defined in claim 1 or claim 2, based on the total weight of the preparation.

6. Preparation according to claim 3, containing 0.001 to 1% by weight of one or‘ more of the alkylamidothiazoles defined in claim 1 or claim 2, based on the total weight of the preparation.

7. Use of one or more alkylamidothiazoles defined in claim 1 or claim 2, or preparations containing one or more such alkylamidothiazoles in the manufacture of a medicament for the cosmetic or dermatological treatment and/or prophylaxis of undesired skin pigmentation.

8. Alkylamidothiazoles as defined in claim 1; cosmetic or dermatological ations as d in claim 3; or use of one or more alkylamidothiazoles as defined in claim 7, substantially as herein described with reference to any one of the embodiments of the invention illustrated in the anying examples.