NZ620042B2 - Composition and method for treating hpv - Google Patents
Composition and method for treating hpv Download PDFInfo
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- NZ620042B2 NZ620042B2 NZ620042A NZ62004212A NZ620042B2 NZ 620042 B2 NZ620042 B2 NZ 620042B2 NZ 620042 A NZ620042 A NZ 620042A NZ 62004212 A NZ62004212 A NZ 62004212A NZ 620042 B2 NZ620042 B2 NZ 620042B2
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- hec
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- cidofovir
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/66—Phosphorus compounds
- A61K31/675—Phosphorus compounds having nitrogen as a ring hetero atom, e.g. pyridoxal phosphate
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/30—Macromolecular organic or inorganic compounds, e.g. inorganic polyphosphates
- A61K47/36—Polysaccharides; Derivatives thereof, e.g. gums, starch, alginate, dextrin, hyaluronic acid, chitosan, inulin, agar or pectin
- A61K47/38—Cellulose; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/0012—Galenical forms characterised by the site of application
- A61K9/0014—Skin, i.e. galenical aspects of topical compositions
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/0012—Galenical forms characterised by the site of application
- A61K9/0034—Urogenital system, e.g. vagina, uterus, cervix, penis, scrotum, urethra, bladder; Personal lubricants
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/0012—Galenical forms characterised by the site of application
- A61K9/0053—Mouth and digestive tract, i.e. intraoral and peroral administration
- A61K9/006—Oral mucosa, e.g. mucoadhesive forms, sublingual droplets; Buccal patches or films; Buccal sprays
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/06—Ointments; Bases therefor; Other semi-solid forms, e.g. creams, sticks, gels
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/14—Particulate form, e.g. powders, Processes for size reducing of pure drugs or the resulting products, Pure drug nanoparticles
- A61K9/19—Particulate form, e.g. powders, Processes for size reducing of pure drugs or the resulting products, Pure drug nanoparticles lyophilised, i.e. freeze-dried, solutions or dispersions
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/70—Web, sheet or filament bases ; Films; Fibres of the matrix type containing drug
- A61K9/7007—Drug-containing films, membranes or sheets
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/12—Antivirals
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/12—Antivirals
- A61P31/20—Antivirals for DNA viruses
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/12—Antivirals
- A61P31/20—Antivirals for DNA viruses
- A61P31/22—Antivirals for DNA viruses for herpes viruses
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P35/00—Antineoplastic agents
Abstract
The present disclosure relates to lyophilized compositions comprising cidofovir, hydroxypropyl methylcellulose (HPMC) or hydroxyethylcellulose (HEC) and optionally a plasticizer. In particular, the present disclosure relates to such compositions which form a sheet-shaped porous solid matrix. The disclosure also relates to methods for producing such compositions. The disclosure further relates to such compositions for use in treating human papillomavirus (HPV) infections and HPV-associated malignancies, in particular HPV lesions and cervical cancer. closure also relates to methods for producing such compositions. The disclosure further relates to such compositions for use in treating human papillomavirus (HPV) infections and HPV-associated malignancies, in particular HPV lesions and cervical cancer.
Description
COMPOSITION AND METHOD FOR TREATING HPV
Field of the invention
The present invention s to the treatment of viral infections. In particular, the
present invention relates to formulations for treating human papillomavirus (HPV)
infections and associated malignancies. The present invention specifically relates to
solid dosage forms for general drug ry, topical application or mucosal delivery.
Background
DNA-viruses such as Herpes, Pox, omavirus, Adenoviruses, Smallpox viruses etc.
can cause many different infectious diseases in . One example, Human
papillomavirus (HPV) is a member of the papillomaviridae family of non-enveloped
DNA s e of infecting humans. Like all papillomaviruses, HPV is strictly
epitheliotropic and establishes productive infections only in the stratified epithelium of
the skin or mucous membranes. While the majority of the nearly 200 known types of
HPV cause no symptoms in most , some types can cause warts, while others
can lead to various s, most notably cervical .
More than 30 to 40 types of HPV are lly transmitted through sexual contact and
infect the anogenital region. Some sexually transmitted HPV types may cause genital
warts. Persistent infection with “high-risk" HPV types, different from the ones that cause
skin warts, may ss to precancerous lesions and invasive cancer. HPV infection is
a cause of nearly all cases of cervical ; however, most infections with these
types do not cause disease.
Most HPV infections in young females are temporary and have little long-term
significance. 70% of infections are gone in 1 year and 90% in 2 years. However, when
infection persists (in 5% to 10% of infected women) there is high risk of developing
cervical precancer (lesions on the cervix), which can progress to invasive cervical
cancer. This process usually takes 15-20 years, providing many opportunities for
detection and treatment of the pre-cancerous condition, often with high cure rates.
While vaccination is an effective way to prevent HPV infection, therapeutic options are
limited, expensive and often not well tolerated. Classical therapeutic approaches
comprise cytodestructive and cytotoxic substances, al methods, laser and
cryotherapy, possibly in combination with immunotherapy. In recent years, the acyclic
nucleoside phosphonate cidofovir has proven to be effective in the treatment of a
variety of clinical manifestations of several DNA-viruses such as Herpes, Pox,
omavirus, Adenoviruses, Smallpox s etc.. in particular, cidofovir has been
used to treat HPV-induced epithelial cell eration. In vitro, treatment of HPV-
positive cells with cidofovir has ed in a concentration- and time-dependent
inhibition of cell proliferation. Different parameters of apoptosis showed that the
mechanism of cell death following treatment with cidofovir is based on apoptosis.
Treatment with enous (systemic) cidofovir has been shown to result in the
stabilization of disseminated papillomatosis. Local intratumor injections of vir in
patients with papillomatous lesions have been shown to result in a complete regression
of the tumor. In addition, cidofovir topical gel has been successfully used for the
treatment of severe, relapsing anogenital HPV lesions and cervical intraepithelial
neoplasia. As cidofovir has been proven to be able to induce apoptosis, the regression
of papillomatous tumors may be due, at least in part, to the induction of sis by
cidofovir.
As illustrated above, s formulations and routes of administration are presently
used for the application of cidofovir. However, each application is presented with
specific drawbacks. Systemic administration of an aqueous cidofovir solution, by
intravenous injection, possibly leads to systemic side effects. Cidofovir concentrations
need to be increased to assure te vir amounts at the target site which
may result in nephrotoxicity. On the other hand, local injections of cidofovir at the target
site may require multiple injections to assure adequate coverage of the target site. As
an alternative to aqueous solutions of cidofovir for injection, creams, gels and films
have been developed for topical applications, which may assure localized application to
the target site. r, the ity of cidofovir in creams is low and its activity
therefore deteriorates fast. In addition, the preparation of films includes a heating step,
which may entail a risk of heat-mediated cidofovir degradation.
It is therefore the ive of the present invention to provide novel cidofovir
formulations which overcome the drawbacks of the currently known formulations.
Summary of the invention
After having med extensive research, the inventors have found that specific
formulations comprising a lyophilized composition sing cidofovir,
hydroxyethylcellulose (HEC) or hydroxypropylmethylcellulose (HPMC) and optionally a
cizer present a le and advantageous alternative for the existing cidofovir
formulations.
Therefore, in an aspect, the invention relates to a sheet-shaped lyophilized composition
comprising:
(a) cidofovir in an amount between 0.1 and 5.0 mg/cm²;
(b) hydroxyethylcellulose (HEC) or hydroxypropylmethylcellulose (HPMC) in an amount
between 1.0 and 17.0 mg/cm²; and ally
(c) a plasticizer in an amount n 0 and 5.0 mg/cm²,
In a preferred embodiment, the lyophilized composition according to the invention
comprises:
(a) cidofovir in an amount between 0.1 and 5 mg/cm², preferably between 2.0 and 5.0
mg/cm²;
(b) HEC in an amount between 1.0 and 17.0 mg/cm², preferably between 7.0 and 10.5
; and
(c) a plasticizer in an amount between 0.5 and 4.0 mg/cm².
Preferably, the HEC is selected from the group consisting of: HEC H4000, HEC 250M,
HEC 250HX and HEC 250HHX, most preferably HEC 250M or HEC 250 HX.
In other preferred embodiments, the lyophilized composition according to the invention
comprises:
(a) cidofovir in an amount n 0.1 and 5 mg/cm²;
(b) HPMC in an amount between 1.0 and 17.0 mg/cm²; and
(c) a plasticizer in an amount between 0.0 and 5.0 mg/cm².
Preferably, the HPMC is selected from the group consisting of HPMC E5, HPMC E15,
HPMC 4000 and HPMC K15, most preferably HPMC E5, or HPMC E15.
The inventors have surprisingly found that such formulations form, after lyophilisation, a
dry, solid, easy to manipulate and malleable porous matrix which presents itself as a
sponge-like structure.
In st to the previously known creams or gels, the composition according to the
invention displays ent cidofovir stability. Hence, and advantageously, the
compositions according to the invention can be stored at ambient (room) temperature,
in contrast to gels and creams, which need to be stored refrigerated.
Moreover, in contrast to the preparation of films a heating step is absent during the
preparation of the compositions according to the present ion, thereby eliminating
the risk of heat-mediated cidofovir degradation.
The addition of a small amount of water to the lyophilized composition according to the
present invention allows for the rapid transformation of the sponge-like structure into a
hesive gel t the need for agitation. It has been found that the speed of
rehydration, as well as the quantity of water needed for rehydration can easily be
modulated by changing the type and the concentration of the r. In particular, it
has been found that the speed of rehydration decreases with increasing concentration
of the polymer. Depending of whether a slow rehydration is needed (e.g. for slow
release applications) a higher concentration of the polymer can be used in the
compositions according to the invention. The inventors have also found that HPMC-
based sponges require a lower amount of water for rehydration than HEC-based
s, which might influence the mode of administration. HPMC-based sponges
may be administered directly, i.e. without being rehydrated before application.
Rehydration which may take place in situ, in the uterus, with the aid of a minimal
amount of vaginal fluids. This is especially true for HPMC E5, HPMC E15, HPMC 4000
and HPMC K15, most particularly for HPMC E5, and HPMC E15.
The HEC-based sponges on the other hand may be rehydrated prior to administration,
resulting in a ke composition, which can subsequently be applied into the vagina
or cervix of the subject. Rehydration of the HEC-based compositions of the invention
can be done with a larger quantity of water than for the ased compositions.
It has further been found that the viscosity of the gel which is ed after rehydration
of the compositions according to the invention is ined by the type and the
concentration of the polymer (not by the type or the concentration of the plasticizer).
This allows for easy modulation of the viscosity depending on the needs by changing
the type and concentration of the polymer. In ular, the inventors have found that
using the same conditions and amounts of components, the HEC-based sponges are
more viscous after rehydration than HPMC-based sponges and present themselves as
gels. This is especially true for HEC H4000, HEC 250M, HEC 250HX and HEC
250HHX, most particularly for HEC 250M and HEC 250 HX.
The differences in viscosity are important in determining the way of applying the
composition of the invention to the subject. The advantage of the HPMC-based
compositions, being less viscous, is that less water is needed for the in situ formation
of a gel-like ure. They can therefore easily be applied internally to the t
t the need for addition of water to invoke ation. Said rehydration will take
place in situ in the body cavity of the subject, using bodily fluids. The HEC-based
itions are more viscous and have the advantage to form a gel, which can be
easier applied topically to e.g. the anogenital region.
The invention therefore also provides a gel-like composition obtained after rehydration
of the lyophilized itions according to the invention, especially of the HEC-based
lized compositions according to the invention.
In an embodiment, the plasticizer in the lyophilized composition according to the
invention, if present, is selected from the group consisting of polyethylene glycol 400 or
4000 (PEG 400 or 4000) and propylene glycol (PG). In a preferred embodiment, the
plasticizer in the composition according to the invention, if present, is PEG, more
preferably PEG 400.
In another embodiment, the lyophilized composition according to the invention further
comprises water in an amount between 1 and 10 %, preferably between 1 and 8
weight%.
In an embodiment, the lyophilized composition ing to the invention further
comprises NaOH. NaOH is generally added to the composition before lyophilization in
order to promote the solubilization of cidofovir. In a further embodiment, the
compositions according to the invention have a pH between 6 and 8, preferably
between 6.5 and 7.5 before lization and after rehydratation of the matrix.
Preferably, the HPMC in the compositions according to the invention is selected from
the group consisting of HPMC E5, HPMC E15, HPMC 4000 and HPMC K15; the HEC
in the compositions according to the invention is selected from the group consisting of:
HEC H4000, HEC 250M, HEC 250HX and HEC 250HHX.
The inventors have shown that in contrast to lyophilized placebo compositions
comprising various ose derivatives such as HPMC, NaCMC, or HEC, which all
form malleable s, only HPMC- and HEC-based itions are capable of
forming malleable sponges after lyophilization when cidofovir is added.
Unexpectedly, other tested compositions, based on other polymers, such as NaCMC,
Carbomer 974P or HPC in the presence of cidofovir do either not form malleable
sponges, or t other unfavorable characteristics, such as being very rigid and/or
brittle, being too sticky or not nt at all, g structural defects or show slow
or ult ation. The inventors have shown that the addition of cidofovir alters
the structural and functional characteristics of the lyophilized s.Furthermore, the
inventors have shown that the on of cidofovir in the compositions according to the
invention alters the pore structure of the sponge, in comparison with placebo sponges.
In particular, it was found that cidofovir seems to weaken the pore structure, especially
in case of the HPMC based compositions.
In one aspect, the invention relates to the lyophilized composition as described herein
for use in topical drug delivery especially for ng all pathologies linked to infections
with human DNA-viruses such as Herpes, Pox, omavirus, Adenoviruses,
Smallpox viruses, preferably by HPV, CMV, BK-virus, Smallpox, HSV, or VZV, or
accompanying pathologies such as those selected from the group comprising: virus
induced lesions or warts of the vagina, cervix, anogenital region, mucosa, epithelium of
the oral sphere, or skin, precancerous lesions and/or sms or cancers caused by
viral infection, more specifically to the cervix or the mucosal surface of the cervix. In
particular, HPV infections and pathologies are envisaged. The term “pathologies linked
to HPV or DNA-virus infections” encompasses, but is not limited to: HPV or DNA-virus
induced lesions or warts of the cervix, uterus, anogenital region, or skin, precancerous
lesions and/or neoplasms or cancers caused by HPV or DNA-virus infection, more
specifically to the cervix or the mucosal e of the cervix. In another aspect, the
invention relates to the lyophilized composition as described herein for use in treating
human papillomavirus (HPV) infection. In yet another aspect, the invention relates to
the lyophilized composition as described herein for use in ng cervical cancer. The
invention also provides for methods of treating HPV or rus ion, using the
lyophilized compositions according to the invention. Administration of said
compositions can be done directly, by inserting the lyophilized composition into the
cervix, e.g. on a l inserter or fixed to a vaginal or uteral cap. atively, the
composition according to the present invention can be rehydrated prior to its
administration and subsequently in the cervix applied as a gel-like composition. The
treatment can be ed a number of times in order to prevent, reduce or eliminate
the pathologies linked to HPV or DNA-virus infections as defined herein, more in
ular HPV or DNA-virus infections, or HPV or DNA-virus induced lesions,
precancerous lesions, or cancers, especially al .
In a further aspect, the invention relates to methods for producing the lyophilized
composition as described herein, comprising the steps of:
(a) dispersing a polymer in water to obtain a homogenized composition;
(b) optionally dispersing a plasticizer in the ition obtained in step (a) to obtain a
homogenized composition;
(c) dispersing cidofovir and optionally adding NaOH 2M solution in the composition
obtained in step (b) or alternatively the composition ed in step (a) if no plasticizer
is added to obtain a homogenized composition;
(d) lizing the composition obtained in step (c).
In a preferred embodiment of the method of the invention,
(a) cidofovir is added in an amount n 0.1 and 5.0 mg/cm²;
(b) hydroxyethylcellulose (HEC) or hydroxypropylmethylcellulose (HPMC) is added in
an amount between 1.0 and 17.0 mg/cm²; and optionally
(c) a plasticizer is added in an amount between 0 and 5.0 mg/cm²,
wherein between 0.5 and 4.0 mg/cm² of said plasticizer is present when HEC is used.
In a particularly preferred embodiment of the method according to the invention,
(a) cidofovir is added in an amount between 0.1 and 5 mg/cm², preferably between 2.0
and 5.0 mg/cm²;
(b) HEC is added in an amount between 1.0 and 17.0 mg/cm², preferably between 7.0
and 10.5 mg/cm²; and
(c) a plasticizer is added in an amount between 0.5 and 4.0 mg/cm².
Preferably, the HEC in the compositions according to the invention is selected from the
group consisting of: HEC H4000, HEC 250M, HEC 250HX and HEC 250HHX, most
preferably HEC 250M and 250HX.
In a r embodiment, the homogenized composition obtained in step (c) comprises
NaOH in an amount between 0.10 to 0.30 weight%, ably about 0.20 or 0.21 %,
before lyophilisation.
In other preferred ments, the lyophilized composition according to the invention
ses:
(a) cidofovir is added in an amount between 0.1 and 5 mg/cm²;
(b) HPMC is added in an amount between 1.0 and 17.0 mg/cm²; and
(c) a plasticizer is added in an amount between 0.0 and 5.0 mg/cm².
Preferably, said HPMC is selected from the group consisting of HPMC E5, HPMC E15,
HPMC 4000 and HPMC K15, most preferably HPMC E5 and E15.
In a further embodiment, the homogenized composition obtained in step (c) comprises
NaOH in an amount between 0.10 to 0.30 weight%, preferably about 0.20 or 0.21 %,
before lyophilisation.
Preferably, said plasticizer is selected from the group consisting of polyethylene glycol
400 or 4000 (PEG 400 or 4000) and propylene glycol (PG). In a preferred embodiment,
the plasticizer in the composition according to the ion, if t, is PEG, more
preferably PEG 400.
In a further embodiment, the homogenized composition obtained in step (c) comprises
between 0.35 and 3.5 weight% of HEC or HPMC. In particular embodiments, the
homogenized composition obtained in step (c) comprises n 1 and 2.5 weight%
HEC and between 0.2 and 1 % plasticizer. In other particular embodiments the
nized composition obtained in step (c) comprises between 0.35 and 3.5
weight% HPMC and between 0 and 1 weight% plasticizer. In yet another embodiment,
the homogenized composition of step (c) comprises between 0.10 and 1.5 weight%
cidofovir. In an embodiment, the homogenized composition obtained in step (c) has a
pH between 6 and 8, preferably between 6.5 and 7.5.
In a further ment, the homogenized composition obtained in step (c) comprises
NaOH in an amount n 0.10 to 0.30 weight%, preferably about 0.20 or 0.21 %,
before lyophilisation.
In an embodiment, the lyophilization for producing the compositions according to the
invention can be performed in a crystallizer, or in a mould, or in any other known device
or reactor that can be used to make a sheet-like lyophilized composition of the
invention.
Another aspect of the invention relates to a sheet-shaped solid porous malleable matrix
obtained by lyophilization of an aqueous composition, said aqueous composition
comprising between 0.35 and 3.5 weight% HEC or HPMC, between 0 and 1 weight%
plasticizer, and between 0.10 and 1.5 weight% cidofovir.
In particular embodiments this matrix comprises between 1 and 2.50 weight% HEC,
between 0.2 and 1 % plasticizer, and between 0.10 and 1.5 weight% cidofovir.
In other particular embodiments this matrix comprises between 0.35 and 3.50 weight%
HPMC, between 0 and 1 weight% plasticizer, and between 0.10 and 1.5 weight%
cidofovir.
In an embodiment, this matrix ses between 1 and 17 mg/cm² HEC or HPMC,
between 0 and 5 mg/cm² plasticizer, and between 0.1 and 5 mg/cm² cidofovir.
In particular embodiments, this matrix comprises between 7 and 10.5 mg/cm² HEC,
between 1.5 and 5 mg/cm² plasticizer, and between 0.1 and 5 mg/cm² cidofovir,
preferably with HEC 250M or HEC 250HX as the r.
In other particular embodiments, this matrix comprises between 1 and 17 mg/cm²
HPMC, between 0 and 5 mg/cm² plasticizer, and n 0.1 and 5 mg/cm² cidofovir,
preferably with HPMC E5 or E15 as the r.
A further aspect of the invention s to a drug delivery applicator, comprising the
sheet-shaped lyophilized composition or the malleable matrix according to the
invention as described herein disposed on a drug-impermeable barrier.
In an embodiment, the drug-impermeable barrier is a cap that is configured to fit over
an outer periphery of a cervix.
Brief ption of the figures
Figure 1: HPLC chromatogram of cidofovir showing absorbance (AU) in function of
retention time (min). (A) togram of calibrated cidofovir; (B) chromatogram of
cidofovir from a lyophilized composition comprising HPMC E5 according to an
embodiment of the invention at T0; (C, D, E, F and G) chromatogram of cidofovir from
a lized composition according to an embodiment of the invention respectively at
T1, T3, T6, T9 and T12, i.e. after 1, 3, 6, 9 and 12 month(s) of storage at 45 °C.
Figure 2: HPLC togram of vir showing absorbance (AU) in function of
ion time (min). (A) chromatogram of cidofovir from a lyophilized composition
comprising HEC 250HX ing to an embodiment of the invention at T0; (B and C)
chromatogram of cidofovir from a lyophilized composition according to an embodiment
of the invention at T1 and T3, i.e. after 1 and 3 months of storage at 45°C.
Figure 3: Diffusion cs of cidofovir, as measured with a Franz diffusion cell,
showing the percentage (%) of diffused cidofovir in function of time (hours). (A)
diffusion kinetics of cidofovir in various lized compositions according to an
embodiment of the invention (HPMC E5-based) in comparison to carbomer gel (prior
art); (B) diffusion cs of cidofovir in various NaCMC-based compositions in
comparison to carbomer gel (prior art); (C) diffusion kinetics of cidofovir in various HEC
250M and 250HX-based compositions in comparison to carbomer gel (prior art).
Figure 4: Scanning on microscopy (SEM) at 25x magnification of the external
layer of compositions comprising HPMC E5 according to an embodiment of the
invention. (left image) SEM of a composition ing to an embodiment of the
invention comprising PEG 400 as a cizer; (right image) SEM of a composition
according to an embodiment of the invention comprising PG as a plasticizer.
Figure 5: Scanning electron microscopy (SEM) at 100x magnification of compositions
comprising HPMC E5. (A) SEM of a placebo composition comprising PEG 400 (left
image) or PG (right image) as a plasticizer; (B) SEM of a composition according to an
embodiment of the invention comprising PEG 400 (left image) or PG (right image) as a
plasticizer.
Figure 6: Scanning on microscopy (SEM) at 4000x magnification of compositions
comprising HPMC E5. (A) SEM of a placebo composition (left image) or a composition
according to an embodiment of the invention (right image) each comprising PEG 400
as a plasticizer; (B) SEM of a placebo composition (left image) or a composition
according to an embodiment of the invention (right image) each sing PG as a
plasticizer.
Figure 7: Scanning electron microscopy (SEM) of placebo compositions comprising
HPMC E5 as polymer and PEG 400 as plasticizer. (A) SEM at 25x magnification of the
external layer of a composition comprising 7,95 mg/cm² (left image) or 15,91 mg/cm²
(right image) of r. (B) SEM at 100x magnification of a composition comprising
7,95 mg/cm² (left image) or 15,91 mg/cm² (right image) of polymer.
Figure 8: Scanning electron microscopy (SEM) of o compositions comprising
HPMC E5. (A) SEM at 25x magnification of the external layer of a ition
sing 0 mg/cm² (left image) or 1,98 mg/cm² (middle image) or 3,97 mg/cm² (right
image) of PEG 400 as plasticizer. (B) SEM at 100x magnification of a composition
comprising 0 mg/cm² (left image) or 1,98 mg/cm² e image) or 3,97 mg/cm² (right
image) of PEG 400 as plasticizer.
Figure 9: Scanning electron microscopy (SEM) at 100x magnification of a placebo
composition (A) or a compositon according to an embodiment of the invention (B)
comprising HPMC E5 (left image) or HPMC 4000 (middle image) or HPMC K15 (right
image) as polymer.
Figure 10: Scanning electron microscopy (SEM) of compositions comprising HEC
250HX. (A) SEM at 25x magnification of the external layer of a placebo composition
comprising 1,98 mg/cm² of PEG 400 as plasticizer (left image) or a compositon
according to an embodiment of the ion comprising 1.98 mg/cm² of PEG 400 as
plasticizer (right image). (B) SEM at 100x magnification of a placebo composition
comprising 1,98 mg/cm² of PEG 400 as plasticizer (left image) or a compositon
according to an embodiment of the invention comprising 1.98 mg/cm² of PEG 400 as
plasticizer (right image).
Figure 11: Scanning electron microscopy (SEM) of compositions comprising HEC
250HX as polymer. SEM of a placebo composition at 4000x magnification (left image)
or a ition according to an embodiment of the invention at 750x magnification
(middle image) or a composition according to an embodiment of the invention at 4000x
magnification (right image) each comprising PEG 400 as a plasticizer.
Figure 12: Viscosity of rehydrated lized compositions comprising NaCMC or
compositions comprising HPMC according to an embodiment of the invention. Viscosity
(Pa.s) is shown in function of rotation speed (s-1). (top graph) general overview; (bottom
graph) detail of the indicated portion in the top graph. The order of the references
s the order of the curve in the figure, starting from the top curve.
Figure 13: Viscosity of ated lyophilized compositions comprising NaCMC or
itions comprising HPMC E5 according to an embodiment of the invention in
function of the plasticizer. Viscosity (Pa.s) is shown in function of rotation speed (s-1).
The order of the references follows the order of the curve in the figure, starting from the
top curve.
Figure 14: Comparison of the viscosity of a gel comprising HEC 250M 4% (without
lyophilization), a sponge comprising HEC 250M 4% after rehydratation with water ad
3g and a carbomer gel with vir. Viscosity (Pa.s) is shown in on of rotation
speed (s-1). (top graph) general overview; m graph) detail of the indicated n
in the top graph. The order of the references follows the order of the curve in the ,
starting from the top curve.
Figure 15: Comparison of the viscosity of a gel comprising HEC 250HX 3% (without
lyophilization), a sponge comprising HEC 250HX 3% after rehydratation with water ad
3g and a carbomer gel with cidofovir. Viscosity (Pa.s) is shown in function of on
speed (s-1). (top graph) general overview; (bottom graph) detail of the indicated portion
in the top graph. The order of the references follows the order of the curves in the
figure, starting from the top curve.
Figure 16: Viscosity of rehydrated lized compositions comprising different types
of HPMC or HEC ing to an embodiment of the invention. Compositions are
classified in function of their viscosity. Viscosity (Pa.s) is shown in function of on
speed (s-1). (top graph) general overview; (bottom graph) detail of the indicated portion
in the top graph. The order of the references follows the order of the curves in the
figure, starting from the top curve.
Figure 17: Differential scanning calorimetry of dehydrated cidofovir in powder form in
comparison with compositions according to an embodiment of the invention. Heat flow
(mW) is shown in function of time (min) and temperature (°C).
Detailed description
Before the present method and products of the invention are described, it is to be
understood that this invention is not limited to particular methods, components,
products or ations described, as such methods, components, products and
combinations may, of course, vary. It is also to be understood that the terminology
used herein is not intended to be limiting.
As used herein, the singular forms "a", "an", and "the" include both singular and plural
referents unless the context clearly dictates ise.
The terms "comprising", "comprises" and "comprised of" as used herein are
synonymous with "including", "includes" or "containing", "contains", and are inclusive or
nded and do not exclude additional, non-recited members, elements or method
steps. It will be appreciated that the terms "comprising", "comprises" and "comprised
of" as used herein se the terms "consisting of", "consists" and "consists of".
The recitation of numerical ranges by endpoints includes all numbers and ons
subsumed within the respective ranges, as well as the recited nts.
The term "about" or “approximately” as used herein when referring to a measurable
value such as a parameter, an amount, a al duration, and the like, is meant to
encompass variations of +/-10% or less, preferably +/-5% or less, more preferably +/-
1% or less, and still more preferably +/-0.1% or less of and from the specified value,
insofar such ions are appropriate to perform in the disclosed invention. It is to be
understood that the value to which the modifier "about" or “approximately” refers is
itself also ically, and preferably, disclosed.
s the terms “one or more” or “at least one”, such as one or more or at least one
member(s) of a group of members, is clear per se, by means of further exemplification,
the term encompasses inter alia a reference to any one of said members, or to any two
or more of said members, such as, e.g., any ≥3, ≥4, ≥5, ≥6 or ≥7 etc. of said
members, and up to all said members.
All references cited in the present specification are hereby incorporated by reference in
their ty. In particular, the teachings of all references herein specifically referred to
are incorporated by reference.
Unless otherwise defined, all terms used in disclosing the invention, including technical
and scientific terms, have the meaning as commonly understood by one of ordinary
skill in the art to which this invention belongs. By means of further ce, term
tions are included to better appreciate the teaching of the present invention.
In the following passages, different aspects of the invention are defined in more detail.
Each aspect so defined may be combined with any other aspect or aspects unless
clearly indicated to the contrary. In particular, any feature ted as being preferred
or advantageous may be combined with any other feature or features indicated as
being preferred or advantageous.
Reference throughout this specification to “one embodiment” or “an embodiment”
means that a particular feature, structure or characteristic described in tion with
the embodiment is included in at least one ment of the present invention. Thus,
ances of the phrases “in one embodiment” or “in an ment” in various
places throughout this specification are not necessarily all referring to the same
ment, but may. Furthermore, the particular features, structures or
characteristics may be combined in any suitable manner, as would be apparent to a
person skilled in the art from this disclosure, in one or more embodiments. Furthermore,
while some embodiments described herein include some but not other features
included in other embodiments, combinations of features of different embodiments are
meant to be within the scope of the invention, and form different embodiments, as
would be understood by those in the art. For e, in the appended , any of
the claimed embodiments can be used in any combination.
The invention relates to a sheet-shaped lyophilized composition comprising
(a) cidofovir in an amount between 0.1 and 5 mg/cm²;
(b) hydroxyethylcellulose (HEC) or hydroxypropylmethylcellulose (HPMC) in an amount
between 1.5 and 17 mg/cm²; and optionally
(c) a biocompatible plasticizer in an amount between 0 and 5 .
Particularly preferred embodiments of compositions ing to the invention are
listed in Tables A to D. Tables A-D list the concentrations of the excipients after
lyophilization in mg/cm² and before lyophilization in weight% (with HPMC as polymer
and without plasticizer in Table A; with HPMC as polymer and with PEG 400 as
plasticizer in Table B; with HPMC as polymer and with PG as plasticizer in Table C;
with HEC as polymer and PEG 400 as plasticizer in Table D). Values in Tables A to D
are ted with a deviation of ±10% of each specific value (e.g. 3.97 mg/cm² HPMC
E5 ±10% ranges between 3.97-0.397 mg/cm² and 3.97+0.397 mg/cm²; 0.42% PEG
400 ±10% ranges between 0.42-0.042% and 0.42+0.042%).
Table A
HPMC vir total weight
Type mg/cm² % mg/cm² % g
E5 3.97±10% 0.83±10% 4.77±10% 1±10% 6
E5 7.95±10% 1.43±10% 4.77±10% 0.86±10% 7
E5 0% 1.67±10% 4.77±10% 1±10% 6
Table B
total
HPMC PEG 400 cidofovir weight
Type mg/cm² % mg/cm² % mg/cm² % g
E5 2.45±10% 1±10% 0.49±10% 0.2±10% 0.36±10% 0.15±10% 10
E5 3.14±10% 1±10% 0.63±10% 0.2±10% 0.94±10% 0.3±10% 5
E5 3.97±10% 0.83±10% 1.98±10% 0.42±10% 0% 1±10% 6
E5 0% 1.33±10% 1.98±10% 0.42±10% 4.77±10% 1±10% 6
E5 7.95±10% 1.43±10% 1.98±10% 0.36±10% 4.77±10% 0.86±10% 7
E5 7.95±10% 1.67±10% 1.98±10% 0% 4.77±10% 1±10% 6
E5 7.95±10% 1.67±10% 1.98±10% 0.42±10% 2.38±10% 0.5±10% 6
E5 11.93±10% 2.5±10% 1.98±10% 0.42±10% 4.77±10% 1±10% 6
E5 12.57±10% 1.67±10% 2.51±10% 0% 1.88±10% 0.25±10% 12
E5 15.91±10% 0% 1.98±10% 0.42±10% 2.387±10% 0.5±10% 6
E5 3.97±10% 0.83±10% 3.97±10% 0.83±10% 4.77±10% 1±10% 6
E5 6.366±10% 1.33±10% 3.97±10% 0.83±10% 0% 1±10% 6
E5 7.95±10% 1.67±10% 3.97±10% 0.83±10% 4.77±10% 1±10% 6
E5 7.95±10% 1.67±10% 3.97±10% 0% 2.38±10% 0.5±10% 6
E5 15.91±10% 3.33±10% 3.97±10% 0.83±10% 4.77±10% 1±10% 6
E15 7.95±10% 1.67±10% 1.98±10% 0.42±10% 4.77±10% 1±10% 6
4000 7.95±10% 1.67±10% 1.98±10% 0% 4.77±10% 1±10% 6
4000 9.54±10% 2±10% 1.98±10% 0% 4.77±10% 1±10% 6
4000 9.54±10% 2±10% 3.97±10% 0.83±10% 4.77±10% 1±10% 6
K15 7.95±10% 1.67±10% 1.98±10% 0.42±10% 4.77±10% 1±10% 6
Table C
total
HPMC PG cidofovir weight
Type mg/cm² % mg/cm² % mg/cm² % g
E5 7.95±10% 1.67±10% 1.98±10% 0.42±10% 2.38±10% 0.5±10% 6
4000 1.98±10% 0% 3.97±10% 0.83±10% 2.38±10% 0.5±10% 6
K15 0% 0.42±10% 3.97±10% 0.83±10% 2.38±10% % 6
Table D
total
HEC PEG 400 cidofovir weight
Type mg/cm² % mg/cm² % mg/cm² % g
250 M 7.95±10% 1.67±10% 1.98±10% 0% 2.38±10% 0.5±10% 6
250 M 9.54±10% 2±10% 1.98±10% 0% 4.77±10% 1±10% 6
250 M 9.54±10% 2±10% 3.58±10% 0.75±10% 4.77±10% 1±10% 6
250 HX 7.95±10% 1.67±10% 1.98±10% 0.42±10% 4.77±10% 1±10% 6
In another embodiment, the invention provides a ceutical composition
comprising a eutically effective amount of the lyophilised composition bed
above and a further pharmaceutically acceptable carrier.
In an aspect of the embodiment, the pharmaceutical composition further comprises an
anti-viral agent. The anti-viral agent can be an Interferon, imiquimod, formaldehyde,
glutaral, cimetidine, 5-fluorouracil, trichloroacetic acid, bleomycin, podofilox,
yllum or any other anti-viral composition usefull for the treatment of HPV
infections, HPV infected tissue, or HPV infected cells.
In still another ment, the invention provides a method of treating HPV infections,
HPV infected tissue or HPV ed cells comprising contacting the infectious site,
infected tissue or cells with the lyophilized composition described herein. In an aspect
of the invention, the method further comprises contacting the cells with an anti-viral
agent. The anti-viral agent can be an Interferon, imiquimod, formaldehyde, glutaral,
cimetidine, 5-fluorouracil, trichloroacetic acid, bleomycin, podofilox, podophyllum, or
any other anti-viral composition usefull for the treatment of HPV infections, HPV
infected tissue, or HPV infected cells. Said further anti-viral agent can be comprised in
the lyophilized composition of the invention, or can be administered simultaneously,
prior to, or following the administration of the lyophilized composition according to the
present invention.
More than 150 types of HPV are acknowledged to exist. Of these, the following have
been fied as types involving risk for cervical cancer: HPV-1, 6, 11, 16, 18, 26, 31,
33, 35, 39, 40, 42, 43, 44, 45, 51, 52, 53, 54, 56, 58, 59, 61, 66, 68, 70, 72, 73, 81, 82,
and CP6108. Types 16 and 18 are generally ledged to cause about 70% of
cervical cancer cases. Together with type 31, they are the prime risk factors for cervical
cancer. In any of the embodiments described herein, said HPV can be HPV 11, HPV16,
HPV18, HPV1, HPV6, any further type of HPV listed above, and any combination
thereof.
Administration of the lyophilized composition according to the present invention, or the
pharmaceutical ition as defined herein can be done directly, i.e. t
ation of the lyophilized matrix, by using e.g. a vaginal inserter, a tampon inserter,
a cervical cap, a cervix-covering y, or any other tool that can be used to position
the lyophilized matrix at the HPV infected tissue or cells. In this scenario, the
lyophilized structure will be rehydrated in situ, using bodily fluids naturally present at
the site of application.
atively, the lyophilized composition according to the present ion, or the
pharmaceutical composition as d herein can be rehydrated prior to use, creating
a gel-like composition which can be topically applied by using e.g. a vaginal cream
inserter, a tampon inserter, a cervical cap, a cervix-covering pessary or any other tool
that can be used to position the gel at the HPV infected tissue or cells.
According to the invention an “effective amount” of the composition or pharmaceutical
composition is that amount effective for treating or lessening the severity of HPV
infections.
lly, HPV infections occur at the skin, , anogenital region, vulva, vagina,
and the cervix (the passage between the vagina and the uterus), in which they may
cause lesions, precancerous lesions, genital warts, polyps, cysts, benign sms
and eventually cancers, that can metastasis into the underlying tissues and circulate
into the blood vessels. Cervical cancer may present with vaginal bleeding, but
symptoms may be absent until the cancer is in its advanced stages. In addition,
lial HPV infections of the oral cavity or sphere are ntly occurring. The oral
cavity or sphere encompasses the lips, mouth, throat, larynx, etc. The preferred target
sites for the (pharmaceutical) composition according to the present invention is
therefore the vulvar, vaginal, and cervical region, especially the mucosal tissue in these
areas, which are most often infected with HPV.
The invention will now be illustrated by means of the following examples, which do not
limit the scope of the invention in any way.
Example 1: lization conditions
Lyophilization of aqueous itions comprising a bioadhesive polymer, and
optionally a cizer and/or cidofovir to obtain a porous malleable matrix was
performed according to the following protocol.
The polymer is dispersed in distilled water under slow agitation until complete
homogenization. Optionally, the obtained dispersion is agitated again until complete
homogenization after the addition of the plasticizer. Cidofovir is sed and a 2M
NaOH stock solution is added to reach pH 7. The obtained mixture is transferred to a
crystallizer and is lyophilized. The lyophilization conditions are the following:
Freezing
Stage Temperature (°C) Time (h) Pressure (bar)
1 - 35 (-30 to -35) 3.0 (2 to 4) ambient
2 - 35 (-30 to -35) 0.5 (0.4 to 0.6) ambient
Primary drying
Stage ature (°C) Time (h) Pressure (bar)
1 - 15 (-10 to -20) 3.0 (2 to 4) 0.8 (0.7 to 0.9)
2 - 10 (-10 to -20) 12.0 (7 to 17) 0.1 (0.05 to 0.15)
ary drying
Stage Temperature (°C) Time (h) re (bar)
1 10 (5 to 15) 2.0 (1 to 3) 0.1 (0.05 to 0.15)
2 10 (5 to 15) 3.0 (2 to 4) 0.1 (0.05 to 0.15)
Example 2: components of the lyophilized composition
The following components and mixtures were evaluated for their capacity to result in
the desired “sponge” structure after lyophilization.
Bioadhesive polymers:
- Hydroxypropylmethylcellulose (HPMC)
HPMC E5 : viscosity: 5 mPa.s (= 5 cp) (aqueous solution of 2%)
HPMC E15 : viscosity: 12-18 mPa.s (aqueous solution of 2%)
HPMC 4000 : ity: 4000-5600 mPa.s (aqueous solution of 2%)
HPMC K15 : viscosity: 11250-21000 mPa.s us solution of 2%)
- Sodium carboxymethyl cellulose (NaCMC)
- Hydroxyethylcellulose (HEC)
HEC Natrosol 250HX: viscosity : 1500-2500 mPa.s (aqueous solution of 1%)
HEC Natrosol 250HHX: viscosity : 3500-5500 mPa.s (aqueous solution of 1%)
HEC Natrosol 250M: viscosity : 500 mPa.s (aqueous solution of 2%)
HEC H4000: ity : 4500-6500 mPa.s (aqueous solution of 2%)
- Carbomer 974P
- Hydroxyproprylcellulose (HPC)
HPC LF: viscosity : 75-150 mPa.s (aqueous solution of 5%)
HPC HF: viscosity : 1500-3000 mPa.s (aqueous solution of 1%)
HPC GF: ity : 0 mPa.s (aqueous solution of 2%)
Plasticizers:
- polyethylene glycol 400 (PEG 400)
- polyethylene glycol 4000 (PEG 4000)
- propylene glycol (PG)
Example 3: evaluation of different lyophilized placebo compositions
ent conditions and concentrations of the components were tested to evaluate the
desired characteristics of the lyophilisate. Desired characteristics are a sponge texture
which is easily malleable when dry and which can be easily and/or rapidly rehydrated
into a gel with intermediate ity (i.e. not too liquid and not too viscous).
The following conditions were kept nt for ease of ison: the diameter of
the crystallizer (4 cm), the quantity of water used for the dispersion of the components
(ad 6 g, meaning water was added to the composition up to 6g of the final composition
before lyophilisation), and the lyophilization cycle.
In first instance, placebo lyophilisates were evaluated (i.e. without cidofovir). Table1
lists the tested concentrations of polymer and plasticizer.
Table 1
Polymer mg/cm² Plasticizer mg/cm² remarks
HPMC E5 3.97 - - malleable sponge
HPMC E5 7.95 - - malleable sponge
HPMC 4000 9.54 - - malleable sponge
HPMC 4000 11.93 - - ble sponge
HPMC E5 3.97 PEG 400 1.98 malleable sponge
HPMC E5 6.36 PEG 400 1.98 malleable sponge
HPMC E5 7.95 PEG 400 1.98 malleable sponge
HPMC E5 11.93 PEG 400 1.98 malleable sponge
HPMC E5 15.91 PEG 400 1.98 malleable sponge
HPMC E5 3.97 PEG 400 3.97 malleable sponge
HPMC E5 6.36 PEG 400 3.97 malleable sponge
HPMC E5 7.95 PEG 400 3.97 malleable sponge
HPMC E5 15.91 PEG 400 3.97 malleable sponge
HPMC E5 31,83 PEG 400 7,95 malleable sponge
HPMC E5 71,62 PEG 400 7,95 malleable sponge
HPMC E15 6.36 PEG 400 1.98 malleable sponge
HPMC E15 7.95 PEG 400 1.98 malleable sponge
HPMC 4000 3.97 PEG 400 1.98 malleable sponge
HPMC 4000 6.36 PEG 400 1.98 malleable sponge
HPMC 4000 7.95 PEG 400 1.98 malleable sponge
HPMC 4000 9.54 PEG 400 1.98 malleable sponge
HPMC 4000 11.93 PEG 400 1.98 malleable sponge
HPMC K15 1.98 PEG 400 0.99 malleable sponge
HPMC K15 7.95 PEG 400 1.98 malleable sponge
HPMC E5 7.95 PEG 4000 0.79 malleable sponge
HPMC E5 7.95 PEG 4000 1.19 malleable sponge
HPMC E5 7.95 PEG 4000 1.98 malleable sponge
HPMC E5 7.95 PG 1.98 malleable sponge
HPMC E5 7.95 PG 3.97 malleable sponge
HPMC E15 6.36 PG 3.97 malleable sponge
HPMC E15 7.95 PG 1.98 malleable sponge
HPMC E15 11.93 PG 3.97 malleable sponge
HPMC 4000 1.98 PG 1.98 malleable sponge
HPMC 4000 3.97 PG 1.98 malleable sponge
HPMC K15 1.98 PG 1.98 malleable sponge
HPMC K15 3.97 PG 1.98 ble sponge
NaCMC 3.18 PEG 400 1.98 malleable sponge
NaCMC 4.77 PEG 400 1.98 malleable sponge
NaCMC 5.57 PEG 400 1.98 ble sponge
NaCMC 6.36 PEG 400 1.98 ble sponge
NaCMC 4.77 PEG 400 3.97 malleable sponge
NaCMC 9.55 PEG 400 3.97 malleable sponge
NaCMC 4.77 PG 1.98 malleable sponge
NaCMC 4.77 PG 3.97 malleable sponge
HEC H4000 9.54 - - malleable sponge
HEC H4000 11.93 - - malleable sponge
HEC H4000 9.54 PEG 400 1.98 malleable sponge
HEC H4000 11.93 PEG 400 1.98 malleable sponge
HEC H4000 11.93 PEG 400 3.97 malleable sponge
HEC 250 M 9.54 - - malleable sponge
HEC 250 M 11.93 - - malleable sponge
HEC 250 M 3.97 PEG 400 1.98 malleable sponge
HEC 250 M 4.77 PEG 400 1.98 malleable sponge
HEC 250 M 7.95 PEG 400 1.98 malleable sponge
HEC 250 M 9.54 PEG 400 1.98 malleable sponge
HEC 250 M 11.93 PEG 400 1.98 malleable sponge
HEC 250 M 11.93 PEG 400 3.97 malleable sponge
HEC 250 HX 7.16 - - malleable sponge
HEC 250 HX 7.95 - - ble sponge
HEC 250 HX 11.93 - - malleable sponge
HEC 250 HX 3.97 PEG 400 1.98 malleable sponge
HEC 250 HX 7.95 PEG 400 1.98 malleable sponge
HEC 250 HX 11.93 PEG 400 1.98 malleable sponge
HEC 250 HHX 4.77 - - malleable sponge
HEC 250 HHX 11.93 - - malleable sponge
HEC 250 HHX 4.77 PEG 400 1.98 ble sponge
HEC 250 HHX 11.93 PEG 400 1.98 malleable sponge
HEC 250 HHX 11.93 PEG 400 3.97 malleable sponge
Carbomer 3.77 PEG 400 1.25 powder
Carbomer 1.47 PEG 400 0.98 powder
Carbomer 1.93 PEG 400 0.99 powder
Carbomer 1.79 PEG 400 0.99 powder
HPC GF 19.09 - - malleable sponge
HPC GF 23.87 - - malleable sponge
HPC GF 19.09 PEG 400 4.77 no sponge texture
HPC GF 23.87 PEG 400 5.96 film
HPC GF 23.87 PEG 400 7.95 film
HPC LF 7.95 PEG 400 1.98 sticky sponge
HPC LF 11.93 PEG 400 1.98 sticky sponge
HPC HF 1.98 PEG 400 0.99 sticky sponge
HPMC E5 7,95 PEG 400 3,97 malleable sponge
HPC GF 1,98
HPMC E5 7,95 PEG 400 3,97 malleable sponge
HPC GF 3,97
HPMC 4000 3,97 PEG 400 3,97 malleable sponge
HPC GF 1,98
HPMC 4000 3,97 PEG 400 3,97 malleable sponge
HPC GF 3,97
HPMC 4000 6,36 PEG 400 3,97 malleable sponge
HPC GF 1,98
HPMC 4000 6,36 PEG 400 3,97 malleable sponge
HPC GF 3,97
HPMC 4000 7,95 PEG 400 3,97 malleable sponge
HPC GF 1,98
HPMC 4000 7,95 PEG 400 3,97 malleable sponge
HPC GF 3,97
HPMC 4000 6,36 PEG 4000 1,19 malleable sponge
HPC GF 3,97
HPMC 4000 7,95 PEG 4000 1,19 malleable sponge
HPC GF 3,97
It was found that compositions based on HPMC, HEC and NaCMC allowed for the
formation of sponges with the desired characteristics, irrespective of the tested
concentrations and the viscosity of the polymer. Compositions based on HPMC E5,
HPMC E15, HPMC 4000, as well as HPMC K15 and compositions based on HEC
H4000, HEC 250 M, HEC 250 HHX, as well as HEC 250 HX allowed for the formation
of sponges. Compositions based on HPC GF allowed for the formation of sponges with
the desired characteristics when used in the absence of a plasticizer, while the
presence of a plasticizer resulted in the formation of bioadhesive films or did not show
a -like texture. The compositions based on er and HPC LF and HPC HF
resulted respectively in powder or in s which were very sticky and not very
malleable. s with the desired characteristics were also obtained with
compositions based on a combination of HPMC E5 and HPC GF and a combination of
HPMC 4000 and HPC GF, all in the presence of plasticizer.
The sponges based on PEG seemed to be more resistant to rupture than the sponges
based on PG. Both, PEG 400 and PEG 4000 may be used to obtain sponges with the
d characteristics, gh higher concentrations of PEG 4000 often result in
britlle s.
Residual amounts of water for the different types of polymers are listed in Table 2.
Table 2
HPMC 1.5 – 8 weight%
NaCMC 5.5 – 14 weight%
HEC 1-6 weight%
HPC 3.5-7 weight%
Carbomer not recoverable for residual water determination
NaCMC-based sponges appear to retain more residual water than for instance HPMC-
based sponges. It has been found that varying the amount of water for preparing the
dispersion (4, 5 and 6 ml) did not influence the final residual amount of water after
lyophilization.
For the evaluation of the speed of rehydration, discs of 1.2 cm diameter were
ated with 50 µl distilled water or the entire sponge ad 3 g with distilled water.
Rehydration of the sponge resulted in the formation of a gel with varying viscosity. The
speed of rehydration depended on the nature and the quantity of the polymer, and
hence can easily be modulated. For instance, it has been found that HEC 250HX- and
250M-based sponges allowed for a faster rehydration than HEC 250HHX- and H4000-
based sponges. Irrespective of the polymer, the speed of rehydration shed with
an increasing tration of polymer.
Example 4: evaluation of different lized cidofovir-containing compositions
Different conditions and concentrations of the components were tested to evaluate the
desired characteristics of the lyophilisate. Desired teristics are a sponge texture
which is easily ble when dry and which can be rapidly rehydrated into a gel with
intermediate viscosity (i.e. not too liquid and not too viscous).
The lyophilization cycle was kept constant for ease of ison, and in most
conditions the diameter of the llizer was 4 cm and the quantity of water added for
the dispersion of the components was ad 6 g.
Tables 3 to 45 list the conditions and evaluation for each of the tested compositions.
Indicated is the concentration in mg/cm² lyophilized sponge and the weight% in the
aqueous composition before lyophilization of the type of polymer (HPMC, NaCMC,
HEC, er or HPC) and the type of plasticizer (no plasticizer, PEG 400 or PG)
and cidofovir. Additionally ted is the amount of NaOH (provided as a 2M stock
solution), the diameter of the crystallizer, and hence the sponge after lyophilization
(circular structure), the weight of the aqueous composition before lyophilization, the
weight of the sponge after lyophilization and the percentage of residual water in the
sponge after lyophilization.
It can be seen that the addition of cidofovir to the composition modifies the properties
of the sponge. Whereas placebo sponges with good quality could be obtained based
on HPMC, HEC and NaCMC as a polymer, it appears that NaCMC-based sponges
with cidofovir become very rigid and brittle (irrespective of concentrations and
conditions). HPMC-based sponges are malleable and of good quality, although they
have a slightly different appearance than their placebo counterparts and present very
faint cracks when the sponge is folded in two. In contrast to their placebo counterparts,
HEC 250HX-based sponges that contain cidofovir need a plasticizer to avoid e of
the sponges (too britlle).
It appears that PG based s are only slightly malleable and brittle, in contrast to
PEG 400 based sponges, which are malleable and of good quality.
It has been found that irrespective of the tested polymer, the speed of rehydration
decreases when the concentration of the polymer ses.
Tables 3 to 45 show some specific examples of compositions that present good-quality
malleable sponges after lyophilisation. Their ation capacities have also been
tested in some cases. The conditions in which non-malleable s were obtained
are also shown for comparison.
Table 3
mg/cm² 7.95
HPMC E5
% 1.43
mg/cm² 4.77
% 0.86
NaOH 2M (mg/cm²) 12.33
diameter lyophilisate (cm) 4
weight before lyophilization (g) 7
weight after lyophilization (mg) 145.4
residual water (%) 7.74
structure malleable sponge
rehydration nd
Table 4
mg/cm² 7.95
HPMC E5
% 1.67
mg/cm² 4.77
% 1
NaOH 2M (mg/cm²) 12.33
diameter lyophilisate (cm) 4
weight before lyophilization (g) 6
weight after lyophilization (mg) 159.6
residual water (%) 7.68
structure malleable sponge
rehydration nd
Table 5
mg/cm² 3.97
HPMC E5
% 0.83
mg/cm² 4.77
Cidofovir
% 1
NaOH 2M (mg/cm²) 12.33
diameter lyophilisate (cm) 4
weight before lyophilization (g) 6
weight after lyophilization (mg) 115.17
residual water (%) nd
structure ble sponge
rehydration nd
Table 6
mg/cm² 2.45
HPMC E5
% 1
mg/cm² 0.36
Cidofovir
% 0.15
mg/cm² 0.49
PEG 400
% 0.2
NaOH 2M (mg/cm²) 1.473
diameter lyophilisate (cm) 7.2
weight before lization (g) 10
weight after lyophilization (mg) 200
residual water (%) 7.073
structure malleable sponge
rehydration very rapid
Table 7
mg/cm² 3.14
HPMC E5
% 1
mg/cm² 0.94
Cidofovir
% 0.30
mg/cm² 0.63
PEG 400
% 0.2
NaOH 2M (mg/cm²) 3.77
diameter lyophilisate (cm) 4.5
weight before lyophilization (g) 5
weight after lyophilization (mg) 100
residual water (%) 8.63
structure malleable sponge
rehydration very rapid
Table 8
mg/cm² 3.97
HPMC E5
% 0.83
mg/cm² 4.77
Cidofovir
% 1
mg/cm² 1.98
PEG 400
% 0.42
NaOH 2M (mg/cm²) 12.33
diameter lisate (cm) 4
weight before lyophilization (g) 6
weight after lyophilization (mg) 128.67
residual water (%) 6.0835
structure malleable sponge
rehydration nd
Table 9
mg/cm² 6.36
HPMC E5
% 1.33
mg/cm² 4.77
Cidofovir
% 1
mg/cm² 1.98
PEG 400
% 0.42
NaOH 2M (mg/cm²) 12.33
diameter lyophilisate (cm) 4
weight before lization (g) 6
weight after lyophilization (mg) 166.485
residual water (%) nd
structure malleable sponge
rehydration nd
Table 10
mg/cm² 7.95
HPMC E5
% 1.43
mg/cm² 4.77
Cidofovir
% 0.86
mg/cm² 1.98
PEG 400
% 0.36
NaOH 2M (mg/cm²) 12.33
diameter lyophilisate (cm) 4
weight before lyophilization (g) 7
weight after lization (mg) 166.6
residual water (%) 7.084
structure malleable sponge
rehydration nd
Table 11
mg/cm² 7.95
HPMC E5
% 1.67
mg/cm² 4.77
Cidofovir
% 1
mg/cm² 1.98
PEG 400
% 0.42
NaOH 2M ²) 12.33
diameter lisate (cm) 4
weight before lyophilization (g) 6
weight after lyophilization (mg) 183.695
residual water (%) 7.51
structure malleable sponge
rehydration nd
Table 12
mg/cm² 7.95
HPMC E5
% 1.67
mg/cm² 2.38
Cidofovir
% 0.5
mg/cm² 1.98
PEG 400
% 0.42
NaOH 2M (mg/cm²) 12.33
diameter lyophilisate (cm) 4
weight before lyophilization (g) 6
weight after lyophilization (mg) 151.24
residual water (%) 5.867
structure malleable sponge
rehydration nd
Table 13
mg/cm² 11.93
HPMC E5
% 2.5
mg/cm² 4.77
Cidofovir
% 1
mg/cm² 1.98
PEG 400
% 0.42
NaOH 2M (mg/cm²) 12.33
er lyophilisate (cm) 4
weight before lyophilization (g) 6
weight after lyophilization (mg) 236.74
residual water (%) nd
structure malleable sponge
rehydration nd
Table 14
mg/cm² 12.57
HPMC E5
% 1.67
mg/cm² 1.88
Cidofovir
% 0.25
mg/cm² 2.51
PEG 400
% 0.33
NaOH 2M (mg/cm²) 3.77
diameter lyophilisate (cm) 4.5
weight before lyophilization (g) 12
weight after lyophilization (mg) nd
al water (%) nd
structure malleable sponge
rehydration nd
Table 15
mg/cm² 15.91
HPMC E5
% 3.33
mg/cm² 2.387
Cidofovir
% 0.5
mg/cm² 1.98
PEG 400
% 0.42
NaOH 2M (mg/cm²) 6.127
diameter lisate (cm) 4
weight before lyophilization (g) 6
weight after lyophilization (mg) 246.91
residual water (%) 4.6845
structure malleable sponge
rehydration nd
Table 16
mg/cm² 3.97
HPMC E5
% 0.83
mg/cm² 4.77
Cidofovir
% 1
mg/cm² 3.97
PEG 400
% 0.83
NaOH 2M (mg/cm²) 12.33
diameter lyophilisate (cm) 4
weight before lyophilization (g) 6
weight after lyophilization (mg) 163.39
al water (%) 6.92
structure malleable sponge
rehydration nd
Table 17
mg/cm² 6.366
HPMC E5
% 1.33
mg/cm² 4.77
Cidofovir
% 1
mg/cm² 3.97
PEG 400
% 0.83
NaOH 2M (mg/cm²) 12.33
diameter lyophilisate (cm) 4
weight before lyophilization (g) 6
weight after lyophilization (mg) 186.82
residual water (%) 4.935
structure malleable sponge
rehydration nd
Table 18
mg/cm² 7.95
HPMC E5
% 1.67
mg/cm² 4.77
% 1
mg/cm² 3.97
PEG 400
% 0.83
NaOH 2M (mg/cm²) 12.33
diameter lyophilisate (cm) 4
weight before lization (g) 6
weight after lyophilization (mg) 208.64
al water (%) 5.2428
structure malleable sponge
rehydration nd
Table 19
mg/cm² 7.95
HPMC E5
% 1.67
mg/cm² 2.38
Cidofovir
% 0.50
mg/cm² 3.97
PEG 400
% 0.83
NaOH 2M (mg/cm²) 6.167
diameter lyophilisate (cm) 4
weight before lyophilization (g) 6
weight after lyophilization (mg) 176.275
residual water (%) nd
structure malleable sponge
rehydration nd
Table 20
mg/cm² 15.91
HPMC E5
% 3.33
mg/cm² 4.77
Cidofovir
% 1
mg/cm² 3.97
PEG 400
% 0.83
NaOH 2M (mg/cm²) 12.33
diameter lyophilisate (cm) 4
weight before lyophilization (g) 6
weight after lyophilization (mg) 304.663
residual water (%) 5.6819
structure malleable sponge
rehydration nd
Table 21
mg/cm² 7.95
HPMC E5
% 1.67
mg/cm² 4.77
Cidofovir
% 1
mg/cm² 1.98
% 0.42
NaOH 2M (mg/cm²) 12.33
diameter lyophilisate (cm) 4
weight before lyophilization (g) 6
weight after lization (mg) 168.675
residual water (%) nd
structure slightly malleable sponge
rehydration nd
Table 22
mg/cm² 7.95
HPMC E5
% 1.67
mg/cm² 2.38
Cidofovir
% 0.5
mg/cm² 1.98
% 0.42
NaOH 2M (mg/cm²) 6.167
diameter lyophilisate (cm) 4
weight before lization (g) 6
weight after lyophilization (mg) 139.05
residual water (%) nd
ure slightly malleable and brittle sponge
rehydration nd
Table 23
mg/cm² 7.95
HPMC E5
% 1.67
mg/cm² 4.77
Cidofovir
% 1
mg/cm² 3.97
% 0.83
NaOH 2M (mg/cm²) 12.33
diameter lyophilisate (cm) 4
weight before lyophilization (g) 6
weight after lyophilization (mg) 184.79
residual water (%) nd
structure slightly malleable and brittle sponge
rehydration nd
Table 24
mg/cm² 7.95
HPMC E15
% 1.67
mg/cm² 4.77
Cidofovir
% 1
mg/cm² 1.98
PEG 400
% 0.42
NaOH 2M (mg/cm²) 12.33
diameter lyophilisate (cm) 4
weight before lyophilization (g) 6
weight after lyophilization (mg) 197.145
al water (%) nd
structure malleable sponge
rehydration nd
Table 25
mg/cm² 7.95
HPMC 4000
% 1.67
mg/cm² 4.77
Cidofovir
% 1
mg/cm² 1.98
PEG 400
% 0.42
NaOH 2M ²) 12.33
diameter lyophilisate (cm) 4
weight before lyophilization (g) 6
weight after lyophilization (mg) 196.65
al water (%) nd
structure malleable sponge
rehydration nd
Table 26
mg/cm² 9.54
HPMC 4000
% 2
mg/cm² 4.77
Cidofovir
% 1
mg/cm² 1.98
PEG 400
% 0.42
NaOH 2M (mg/cm²) 12.33
diameter lyophilisate (cm) 4
weight before lyophilization (g) 6
weight after lyophilization (mg) 193.39
residual water (%) nd
structure malleable sponge
ation nd
Table 27
mg/cm² 9.54
HPMC 4000
% 2
mg/cm² 4.77
Cidofovir
% 1
mg/cm² 3.97
PEG 400
% 0.83
NaOH 2M (mg/cm²) 12.33
diameter lyophilisate (cm) 4
weight before lyophilization (g) 6
weight after lyophilization (mg) 220.75
residual water (%) 3.35
structure ble sponge
rehydration nd
Table 28
mg/cm² 1.98
HPMC 4000
% 0.42
mg/cm² 2.38
Cidofovir
% 0.5
mg/cm² 3.97
% 0.83
NaOH 2M (mg/cm²) 6.167
diameter lisate (cm) 4
weight before lyophilization (g) 6
weight after lyophilization (mg) 68.39
residual water (%) nd
structure malleable sponge
rehydration nd
Table 29
mg/cm² 7.95
HPMC K15
% 1.67
mg/cm² 4.77
Cidofovir
% 1
mg/cm² 1.98
PEG 400
% 0.42
NaOH 2M ²) 12.33
diameter lyophilisate (cm) 4
weight before lyophilization (g) 6
weight after lyophilization (mg) 194.39
residual water (%) nd
structure malleable sponge
rehydration nd
Table 30
mg/cm² 1.98
HPMC K15
% 0.42
mg/cm² 2.38
Cidofovir
% 0.5
mg/cm² 3.97
% 0.83
NaOH 2M (mg/cm²) 6.167
diameter lyophilisate (cm) 4
weight before lyophilization (g) 6
weight after lyophilization (mg) 68.05
al water (%) nd
structure malleable sponge
rehydration nd
Table 31
mg/cm² 7.95
HEC 250M
% 1.67
mg/cm² 2.38
Cidofovir
% 0.5
mg/cm² 1.98
PEG 400
% 0.42
NaOH 2M (mg/cm²) 6.167
diameter lyophilisate (cm) 4
weight before lyophilization (g) 6
weight after lyophilization (mg) 144.34
al water (%) nd
structure malleable sponge
rehydration very rapid
Table 32
mg/cm² 9.54
HEC 250M
% 2
mg/cm² 4.77
Cidofovir
% 1
mg/cm² 1.98
PEG 400
% 0.42
NaOH 2M (mg/cm²) 12.33
diameter lisate (cm) 4
weight before lyophilization (g) 6
weight after lyophilization (mg) 200.56
residual water (%) 4.208
structure malleable sponge
rehydration very rapid
Table 33
mg/cm² 9.54
HEC 250M
% 2
mg/cm² 4.77
Cidofovir
% 1
mg/cm² 3.58
PEG 400
% 0.75
NaOH 2M (mg/cm²) 12.33
diameter lyophilisate (cm) 4
weight before lyophilization (g) 6
weight after lyophilization (mg) 221.69
residual water (%) nd
structure malleable sponge
rehydration nd
Table 34
mg/cm² 7.95
HEC 250 HX
% 1.67
mg/cm² 4.77
Cidofovir
% 1
mg/cm² 1.98
PEG 400
% 0.42
NaOH 2M (mg/cm²) 12.33
diameter lyophilisate (cm) 4
weight before lyophilization (g) 6
weight after lyophilization (mg) 183.155
residual water (%) 3.44
structure malleable sponge
rehydration réhydratation immédiate
Table 35
mg/cm² 7.95
HEC 250 HX
% 1.67
mg/cm² 4.77
% 1
mg/cm² -
PEG 400
% -
NaOH 2M (mg/cm²) 12.33
diameter lyophilisate (cm) 4
weight before lyophilization (g) 6
weight after lyophilization (mg) 149,95
residual water (%) nd
structure brittle sponge
rehydration nd
Table 36
mg/cm² 4.77
NaCMC
% 0.86
mg/cm² 4.77
Cidofovir
% 0.86
NaOH 2M (mg/cm²) 12.33
diameter lyophilisate (cm) 4
weight before lyophilization (g) 7
weight after lyophilization (mg) 113.4
residual water (%) 8.265
structure rigid and e sponge
rehydration nd
Table 37
mg/cm² 4.77
NaCMC
% 1
mg/cm² 4.77
Cidofovir
% 1
NaOH 2M (mg/cm²) 12.33
diameter lyophilisate (cm) 4
weight before lyophilization (g) 6
weight after lyophilization (mg) 113.4
residual water (%) 13.659
structure very rigid and brittle sponge
rehydration nd
Table 38
mg/cm² 3.18
NaCMC
% 0.67
mg/cm² 4.77
Cidofovir
% 1
mg/cm² 1.98
PEG 400
% 0.42
NaOH 2M (mg/cm²) 12.33
diameter lyophilisate (cm) 4
weight before lyophilization (g) 6
weight after lyophilization (mg) 5
residual water (%) 8.635
ure rigid and brittle sponge
rehydration nd
Table 39
mg/cm² 4.77
NaCMC
% 0.86
mg/cm² 4.77
Cidofovir
% 0.86
mg/cm² 1.98
PEG 400
% 0.36
NaOH 2M (mg/cm²) 12.33
diameter lyophilisate (cm) 4
weight before lyophilization (g) 7
weight after lyophilization (mg) 133.25
al water (%) 10.631
structure rigid and brittle sponge
rehydration nd
Table 40
mg/cm² 4.77
NaCMC
% 1
mg/cm² 4.77
Cidofovir
% 1
mg/cm² 1.98
PEG 400
% 0.42
NaOH 2M (mg/cm²) 12.33
diameter lyophilisate (cm) 4
weight before lyophilization (g) 6
weight after lyophilization (mg) 146.65
residual water (%) 6.51
structure rigid and brittle sponge
rehydration nd
Table 41
mg/cm² 6.36
NaCMC
% 1.33
mg/cm² 4.77
% 1
mg/cm² 1.98
PEG 400
% 0.42
NaOH 2M (mg/cm²) 12.33
diameter lyophilisate (cm) 4
weight before lyophilization (g) 6
weight after lyophilization (mg) 149.65
residual water (%) 11.964
structure very rigid and brittle sponge
rehydration nd
Table 42
mg/cm² 4.77
NaCMC
% 1
mg/cm² 4.77
Cidofovir
% 1
mg/cm² 3.97
PEG 400
% 0.83
NaOH 2M (mg/cm²) 12.33
diameter lyophilisate (cm) 4
weight before lyophilization (g) 6
weight after lyophilization (mg) 169.05
al water (%) 6.02
structure very rigid and brittle sponge
rehydration nd
Table 43
mg/cm² 3.18
NaCMC
% 0.67
mg/cm² 2.38
% 0.5
mg/cm² 3.97
% 0.83
NaOH 2M (mg/cm²) 6.167
diameter lyophilisate (cm) 4
weight before lyophilization (g) 6
weight after lyophilization (mg) 90.42
residual water (%) nd
structure slightly rigid and brittle sponge
rehydration nd
Table 44
mg/cm² 4.77
NaCMC
% 1
mg/cm² 4.77
Cidofovir
% 1
mg/cm² 3.97
% 0.83
NaOH 2M (mg/cm²) 12.33
diameter lyophilisate (cm) 4
weight before lyophilization (g) 6
weight after lyophilization (mg) 145.77
al water (%) nd
structure very rigid and brittle sponge
rehydration nd
Table 45
mg/cm² 4.77
NaCMC
% 1
mg/cm² 2.38
Cidofovir
% 0.5
mg/cm² 3.97
% 0.83
NaOH 2M (mg/cm²) 6.167
diameter lyophilisate (cm) 4
weight before lyophilization (g) 6
weight after lyophilization (mg) 108.08
residual water (%) nd
structure very rigid and brittle sponge
rehydration nd
After rehydration, a gel-like composition can be obtained. A preferred ke
ition typically comprises about 2% cidofovir and 4% HEC250M. The
composition typically comprises HCL and NaOH in a quantity to reach a pH value of
7.2. The remaining component of the gel-like composition is water, to reach 100%. If
required, a antibacterial agent could be added to further improve the shelf-life. One
example is Benzyl alcohol (about 2%).
Example 5: water content in the sponge
The residual water in the sponge after lyophilization was measured by the Karl Fisher
method (Metler DL35). From Tables 46 it can be seen that the percentage residual
water varies in function of the polymer as well as in function of the presence of vir.
Table 46
HPMC E5 PEG 400 PG Cidofovir % water n
7.95 1.98 3,08 ± 1,36 3
6.36 3.97 1.95 1
7.95 3.97 1.83 1
.91 3.97 3,18 ± 1,94 5
31.83 3.97 1,89 ± 0,401 3
7.95 4.77 7.68 ± 1,34 3
3.97 1.98 4.77 6.09 ± 0,35 2
7.95 1.98 2.38 5.87 1
7.95 1.98 4.77 7.39 ± 1,44 7
.91 1.98 2.38 4.68 1
.91 1.98 4.77 5.80 ± 1,08 2
3.97 3.97 4.77 6,91 ± 1,71 3
6.36 3.97 4.77 4.94 ± 0,5 2
7.95 3.97 4.77 5.25 ± 1,12 3
.91 3.97 4.77 5,18 ± 0,67 4
7.95 1.98 2.69 1
.58 2.63 3.33 1
11,93 2.94 3.18 ± 0,40 2
7.95 3.97 2.76 1
.91 3.97 5.12 1
HEC 250 PEG 400 PG Cidofovir % water n
7.95 1.98 4,80 ± 1,4 15
7.95 1.98 4.77 3,44 ± 0,33 3
HEC 250 M PEG 400 PG Cidofovir % water n
9.54 1.98 4,44 ± 0,69 5
9.54 1.98 4.77 4,21 ± 1,26 4
HPMC 4000 PEG 400 PG Cidofovir % water n
9.54 1.98 1,03 ± 0,06 3
9.54 3.97 4.77 3,35 ± 0,01 2
NaCMC PEG400 PG Cidofovir % water n
4,77 1,98 9,34 ± 1,98 3
.57 1,98 8.19 1
4.77 3,97 6.86 1
4,77 4,77 11.86 ± 3,18 1
3.18 1.98 4.77 8.64 ± 0,02 2
4.77 1,98 4,77 6.51 1
6,36 1,98 4,77 11.96 1
4.77 3,97 4,77 6.02 1
4.77 1,98 8.33 1
The residual water content is lower in HPMC- and sed sponges than in
NaCMC-based sponges.
It has been established that the quantity of water which was added to obtain the
aqueous composition before lyophilization (4, 5 or 6 ml were tested) did not influence
the amount of residual water after lyophilization.
Example 6: uniformity of cidofovir
The uniformity of cidofovir presence inside the sponges has been tested by cutting
sponges in 4 parts and testing the cidofovir dose by chromatography (HPLC). The
ions are as follows:
- stationary phase: LiChrospher® 100 RP-18 e pped) (5 µm) in an analytical
cart® column of 250 mm x 4 mm, d.i.
- mobile phase: HPLC buffer pH 6.5 / ACN (90/10, m/m)
- flow rate: 1 ml/min
- temperature: 30°C
- detection: ophotometric adsorption, UV 275 nm
- injection volume: 20 µl
The composition of the HPLC buffer is:
- 5 mM tetrabutyl ammonium hydrogen sulphate
- 5 mM ammonium dihydrogen phosphate
- pH adjusted to 6.5 with ammonium
Table 47 list the results for HPMC E5-based sponges, as a tage of the
theoretically expected dose.
Table 47
lot No location % of theoretical lot No location % of theoretical
10C24-3 side 95.67 10C24-4 side 101.03
10C24-3 side 112.77 10C24-4 side 100.57
3 side 87.86 10C24-4 side 95.24
10C24-3 center 92.39 10C24-4 center 95.83
Example 7: stability of cidofovir
Lyophilized sponges have been bagged (PET/Al/EZ) and shielded from humidity, light
and . The sponges were kept at three different temperatures: 4°C, 25°C and
45°C. Figure 1 shows the chromatograms of sponges that contained HPMC E5 (7.95
mg/cm²), PEG 400 (1.98 mg/cm²) and cidofovir (4.77 mg/cm²). s 1A, 1B, 1C, 1D,
1E, 1F and 1G represent respectively the chromatograms of the calibration (100 µg/ml),
the sponge at T0 and the sponge at T1, T3, T6, T9 and T12 (i.e. respectively after one,
three, six, nine and twelve month(s)) at 45°C. Figures 2A, 2B and 2C ent the
chromatograms of a sponge comprising HEC 250HX (7.95 mg/cm²), PEG 400 (1.98
mg/cm²) and cidofovir (4.77 mg/cm²) respectively at T0, T1 and T3 (i.e. after one and
three month(s)) at 45°C. It can be seen that the peak area is small (irrespective of the
ature at which the sponges are kept) and that the peak is equally present in the
calibration chromatogram. Hence, the sponges are stable under the conditions tested.
Example 8: diffusion of vir
Diffusion of cidofovir was measured with a Franz ion cell. A lyophilisate of 1.2 cm
er was placed in the cell and rehydrated with 350 µl of a phosphate buffer (pH 5,
37°C). The diffusion kinetics are evaluated by HPLC in doses of 1 ml. Polymers were
varied (HPMC E5, NaCMC, HEC 250HX and HEC 250M) as well as the presence or
absence of plasticizer (PEG 400). The concentration of cidofovir was kept constant.
From figures 3A, 3B and 3C, it seems that a plateau is reached for HPMC-based
sponges after 1 hour, for NaCMC- and HEC 250M-based sponges after 2 hours and for
HEC 250HX-based sponges after 3 hours. Nevertheless, cidofovir diffuses fast from
irrespective compositions.
Example 9: copic analysis
The structure of the sponges based on HPMC E5, HPMC 4000, HPMC K15 and HEC
250HX was evaluated by scanning electron microscopy. Pore structure was compared
between sponges with different type of r, s with different concentration of
the polymer, sponges with or without cidofovir, sponges with or without plasticizer as
well as sponges with different plasticizer (PEG 400 or PG) and different concentrations
of plasticizer.
Figures 4 to 8 are pictures of HPMC E5-based sponges at different magnitudes. From
Figure 4, it is apparent that the external layer of PG-based s have more grooves
than PEG 400-based sponges. From Figure 5, it is apparent that the pores are better
organized in PEG 400-based sponges than in PG-based sponges. In confirmation that
PEG 400-based sponges are more resistant, PG appears to lead to a disorganization
of the pore structure, which pores become more fragile. The addition of cidofovir to the
sponges likewise appears to make the pore ure more fragile. From Figure 6, it
appears that the pore surface is smooth in the placebo sponges, whereas the surface
is granular in sponges containing cidofovir, irrespective of whether PEG 400 or PG is
used as plasticizer. It appears that cidofovir is distributed uniformly. From Figure 7, it is
nt that an increase in polymer concentration (from 7,95 to 15.91 mg/cm² HPMC
E5) results in a more dense sponge structure with smaller pores. From Figure 8, it
s that the pore structure is better organized in the presence of PEG 400
plasticizer. In addition, it appears that less s are observed on the external layer
of the sponge and that the pores are smaller by augmenting the concentration of the
PEG 400 plasticizer. Figure 9 shows ng electron microscopy images of sponges
based on HPMC E5, HPMC 4000 or HPMC K15 without or with cidofovir. The
lar weight of the HPMC r appears to affect the pore size: the pores
increase with the molecular weight of the r. Smaller pores are observed in the
ce of cidofovir. The presence of cidofovir also results in less organized pores,
which is even more pronounced in the sponges based on a higher molecular weight
HPMC polymer.
s 10 and 11 represent es of sponges based on HEC 250HX polymer. From
figure 10, it seems that the pores are smaller in the presence of cidofovir. From figure
11, it s that the pore surface is smooth in the placebo sponges, s the
surface is granular in sponges containing vir. It appears that cidofovir is
distributed as crystal form uniformly.
Example 10: porosity and sponge thickness
Experiments have been performed to evaluate the parameters which influence the
thickness of the sponge. It has been found that increasing the quantity of the polymer
in the aqueous composition before lyophilization only slightly increased the thickness of
the sponge after lyophilization. On the other hand, the thickness of the sponge after
lyophilization could be increased substantially by increasing the amount of water which
is added to the mixture before lyophilization. Thicker sponges were obtained with a
mixture of at least 8 g (8, 9, 12, or 15 g). Moreover, it ed that PG-based
compositions allowed for obtaining thicker sponges, whereas PEG 400-based
compositions under the same conditions liquefied. A longer lyophilization cycle may be
needed.
Example 11: viscosity
The ity of the s after rehydration was performed with a rheometer (ARES
G2, TA instrument). The following parameters were kept constant for ease of
ison: sponge surface and amount of water for rehydration (900µl except for
Figures 14 and 15). Measurements were performed at 37°C for 240 s at a rotation
speed from 1 to 100 s-1.
From Figure 12, it is clear that the NaCMC-based s are more viscous than the
HPMC E5-based sponges. No difference in viscosity was observed between different
HPMC E5 concentrations. HPMC E15-based sponges appeared more viscous than
HPMC E5-based sponges (approaching the viscosity of NaCMC-based sponges).
From Figure 13 it is clear that the viscosity is not influenced by the nature of plasticizer
(PEG 400 or PG).
From Figures 14 and 15, it appears that the viscosity of the lyophilized compositions is
not influenced by the lyophilization process. The viscosity of ated HEC 250 M
and HEC 250 HX based sponges is able to the viscosity of HEC 250 M and
HEC 250 HX gels (that were not lyophilized) and approaches the viscosity of a
carbomer gel comprising cidofovir.
From Figure 16, it is clear that the viscosity is influenced by the type of polymer: HMPC
K15, HPMC 4000 (with or without HPC GF), HEC 250 HX, HEC 250 M, HPMC E15
and HPMC E5 are classified following decreasing viscosity. Furthermore, the viscosity
increases with increasing concentration of the polymer.
Example 12: sponge measurements by differential scanning calorimetry
The thermal transitions of lyophilisates and the eventual interaction between the
polymers and cidofovir were evaluated by differential scanning calorimetry (DSC 25
Mettler Toledo, controlled by the TC15 TA Controller). The temperature was increased
by 10°C per minute n 35 and 300°C. From Figure 17, it is clear that cidofovir
dehydrated in powder form shows an endothermal peak at 280°C. This peak is absent
in the metrical analysis of the different sponges because vir is transformed
to salt by the method for ing the lyophilized composition.
Claims (42)
1. A sheet-shaped lyophilized composition comprising (a) cidofovir in an amount between 0.1 and 5 mg/cm²; 5 (b) hydroxyethylcellulose (HEC) or hydroxypropylmethylcellulose (HPMC) in an amount between 1 and 17 mg/cm².
2. The lyophilized composition according to claim 1 further comprising (c) a plasticizer in an amount between 0 and 5 mg/cm².
3. The lyophilized composition according to claim 2 comprising: (a) vir in an amount n 0.1 and 5 mg/cm²; (b) hydroxyethylcellulose (HEC) in an amount between 1 and 17 mg/cm²; and (c) a plasticizer in an amount between 0.5 and 4 mg/cm².
4. The lyophilized composition according to any one of claims 1 to 3, wherein HEC is present in an amount between 7 and 10.5 mg/cm², between 5 and 10 mg/ cm², between 8 and 10 mg/ cm², or between 10 and 16 mg/ cm². 20 5. The lized ition ing to any one of claims 1 to 4, wherein said
HEC is selected from the group consisting of HEC H4000 HEC 250HHX, HEC 250M and HEC 250HX.
6. The lyophilized composition according to claim 5, wherein the HEC is either 25 HEC 250 M or HX.
7. The lyophilized composition according to any one of claims 1 to 6, which presents itself as a high viscosity composition. 30
8. The lyophilized composition according to claim 1 comprising: (a) cidofovir in an amount between 0.1 and 5 mg/cm²; (b) hydroxypropylmethylcellulose (HPMC) in an amount n 1 and 17 mg/cm²; and (c) a plasticizer in an amount n 0 and 5 mg/cm².
9. The lyophilized ition according to claim 8, wherein HPMC is present in an amount between 2.5 and 8 mg/ cm², between 4 and 8 mg/ cm² or between 8 and 15 mg/ cm². 5 10. The lyophilized composition according to claim 8 or 9 wherein said HPMC is selected from the group consisting of HPMC E5, HPMC E15, HPMC 4000 and
HPMC K15.
11. The lized composition ing to claim 10, wherein said HPMC is either 10 HPMC E5 or HPMC E15
12. The lized composition according to any one of claims 8 to 11, which presents itself as a low viscosity composition. 15
13. The lyophilized composition according to any one of claims 2 to 12, when dependent on either claim 2 or 8, wherein said plasticizer is selected from the group consisting of polyethylene glycol 400 (PEG 400), polyethylene glycol 4000 (PEG 4000) and propylene glycol (PG). 20
14. The lyophilized composition according to claim 13, wherein said plasticizer is PEG 400.
15. The lyophilized composition according to any one of claims 1 to 4, wherein water is t in an amount between 1 and 10 weight%, and/or NaOH is 25 present in an amount of between 0.15 and 0.25 weight%.
16. The lyophilized composition according to any of claims 1 to 15, wherein said composition is a porous malleable matrix. 30
17. The lyophilized composition according to any of claims 1 to 16 for use in
18. The lyophilized composition according to claim 17 for use in treating infection with human DNA-virus.
19. The lyophilized composition according to claim 18 for use in treating an infection selected from the list consisting of Herpes virus, Pox virus, Papilloma virus, Adenovirus, Smallpox virus, Human papillomavirus (HPV), and accompanying pathologies.
20. The lyophilized composition according to claim 19 for use in treating an accompanying ogy selected from the group comprising: virus induced lesions or warts of the vagina, cervix, anogenital region, , epithelium of the oral sphere, or skin, precancerous lesions and/or neoplasms or cancers 10 caused by HPV infection, more specifically to the cervix or the l e of the cervix.
21. A gel-like composition obtained after rehydration of the lyophilized composition according to any of claims 1 to 16 for use in medicine.
22. The gel-like composition according to claim 21, for use in treating infections with human DNA-viruses.
23. The gel-like composition according to claim 22, for use in treating a human 20 DNA-virus infection selected from the group comprising , Pox, omavirus, Adenoviruses, Smallpox viruses, Human papillomavirus (HPV) infection and accompanying pathologies.
24. The gel-like ition ing to claim 23, for use in treating an 25 accompanying pathology selected from the group comprising: virus induced lesions or warts of the vagina, cervix, anogenital region, mucosa, epithelium of the oral sphere, or skin, precancerous lesions and/or sms or cancers caused by viral infection, more specifically to the cervix or the mucosal surface of the cervix.
25. The lyophilized composition according to any of claims 3 to 6 for use in treating human DNA virus infection or accompanying pathologies, wherein the composition is rehydrated before administration.
26. The lyophilized ition according to any one of claims 8 to 12, for use in treating human DNA virus infection or anying pathologies , wherein the composition is not rehydrated before administration. 5
27. The lyophilized composition according to any one of of claims 1 to 26, or the gel-like composition of claim 21, for use in topical drug delivery to a site selected from the group comprising the vagina, cervix, anogenital region, mucosa, epithelium of the oral sphere and skin. 10
28. A method for producing the lyophilized composition ing to any of claims 1 to 16, comprising the steps of (a) dispersing HEC or HPMC in water to obtain a homogenized composition; (b) dispersing 0% or more plasticizer in the composition obtained in step (a) to obtain a homogenized composition; 15 (c) dispersing cidofovir and adding 0% or more NaOH 2M solution in the composition obtained in step (b); (d) lyophilizing the composition obtained in step (c), wherein cizer needs to be added in step (b) when HEC is used in step (a). 20
29. The method according to claim 28, wherein the homogenized composition of step (c) comprises between 1 and 17 mg/cm2 of HEC or HPMC.
30. The method according to claim 28 or 29, wherein the homogenized ition of step (c) comprises between 7 and 10.5 mg/cm², between 5 and 10 mg/cm², 25 between 8 and 10 mg/cm², or between 10 and 16 mg/cm² HEC and between 1.5 and 33 mg/cm² plasticizer.
31. The method according to claim 28 or 29, wherein the nized ition of step (c) comprises between 2.5 and 8 mg/cm², between 4 and 8 mg/cm², or 30 between 8 and 15 mg/cm² HPMC and between 0 and 5 mg/cm² plasticizer.
32. The method according to any one of claims 28 to 31, wherein the homogenized composition of step (c) comprises between 0.1 and 5 mg/cm² cidofovir.
33. The method ing to any one of claims 28 to 32, wherein the homogenized composition of step (c) has a pH between 6 and 8.
34. The method according to claim 33, wherein the homogenized composition of 5 step (c) has a pH between 6.5 and 7.5.
35. The method according to claim 33 or 34, wherein the homogenized composition of step (c) comprises NaOH. 10
36. A method of preparing a gel-like composition according to claim 21, comprising the step of rehydrating the lyophilized composition according to any one of claims 1 to 16.
37. A sheet-shaped solid porous malleable matrix obtained by lyophilization of an 15 aqueous composition, said aqueous composition comprising between 1 and 17 mg/cm² HEC or HPMC, between 0 and 5 mg/cm² plasticizer, and between 0.1 and 5 mg/cm² cidofovir, wherein between 1.5 and 3 mg/cm² of said plasticizer is present when HEC is used. 20
38. The matrix according to claim 37, wherein said aqueous composition comprises: - n 7 and 10.5 mg/cm², between 5 and 10 mg/ cm², between 8 and 10 mg/ cm², or between 10 and 16 mg/ cm² HEC, - between 1.5 and 3mg/cm² cizer, and - between 0.1 and 5 mg/cm² cidofovir.
39. The matrix according to claim 37, wherein said aqueous composition comprises: - between 2.5 and 8 mg/ cm², between 4 and 8 mg/ cm² or between 8 and 15 mg/ cm² HPMC, - between 0 and 5 mg/cm² plasticizer, and 30 - n 0.1 and 5 mg/cm² cidofovir.
40. A drug delivery applicator, comprising the sheet-shaped lized ition ing to any one of claims 1 to 16 or the matrix according to any one of claims 37 to 39.
41. The drug delivery applicator according to claim 40, in the form of a cervical cap or a cervix-covering pessary, or in the form of a vaginal inserter, a l cream inserter, or a tampon inserter. 5
42. The drug delivery applicator according to claim 41, wherein the cervix-covering pessary comprises a drug-impermeable barrier preventing the active ingredient from diffusing.
Applications Claiming Priority (5)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US201161508473P | 2011-07-15 | 2011-07-15 | |
EP11174193.0 | 2011-07-15 | ||
US61/508,473 | 2011-07-15 | ||
EP11174193 | 2011-07-15 | ||
PCT/EP2012/063796 WO2013010942A1 (en) | 2011-07-15 | 2012-07-13 | Composition and method for treating hpv |
Publications (2)
Publication Number | Publication Date |
---|---|
NZ620042A NZ620042A (en) | 2015-09-25 |
NZ620042B2 true NZ620042B2 (en) | 2016-01-06 |
Family
ID=
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