NZ619674B2 - Coagulation controlling agents and devices comprising the same - Google Patents
Coagulation controlling agents and devices comprising the same Download PDFInfo
- Publication number
- NZ619674B2 NZ619674B2 NZ619674A NZ61967412A NZ619674B2 NZ 619674 B2 NZ619674 B2 NZ 619674B2 NZ 619674 A NZ619674 A NZ 619674A NZ 61967412 A NZ61967412 A NZ 61967412A NZ 619674 B2 NZ619674 B2 NZ 619674B2
- Authority
- NZ
- New Zealand
- Prior art keywords
- container
- thrombin
- blood
- controlling agent
- coagulation
- Prior art date
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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- A01N1/02—Preservation of living parts
- A01N1/0205—Chemical aspects
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- A01N1/0226—Physiologically active agents, i.e. substances affecting physiological processes of cells and tissue to be preserved, e.g. anti-oxidants or nutrients
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/15—Devices for taking samples of blood
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/15—Devices for taking samples of blood
- A61B5/150007—Details
- A61B5/150015—Source of blood
- A61B5/15003—Source of blood for venous or arterial blood
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- A—HUMAN NECESSITIES
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- A61B5/15—Devices for taking samples of blood
- A61B5/150007—Details
- A61B5/150351—Caps, stoppers or lids for sealing or closing a blood collection vessel or container, e.g. a test-tube or syringe barrel
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- A—HUMAN NECESSITIES
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- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/15—Devices for taking samples of blood
- A61B5/150007—Details
- A61B5/150374—Details of piercing elements or protective means for preventing accidental injuries by such piercing elements
- A61B5/150381—Design of piercing elements
- A61B5/150389—Hollow piercing elements, e.g. canulas, needles, for piercing the skin
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- A—HUMAN NECESSITIES
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- A61B5/15—Devices for taking samples of blood
- A61B5/150007—Details
- A61B5/150374—Details of piercing elements or protective means for preventing accidental injuries by such piercing elements
- A61B5/150381—Design of piercing elements
- A61B5/150473—Double-ended needles, e.g. used with pre-evacuated sampling tubes
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- A—HUMAN NECESSITIES
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- A—HUMAN NECESSITIES
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- A61B5/15—Devices for taking samples of blood
- A61B5/153—Devices specially adapted for taking samples of venous or arterial blood, e.g. with syringes
- A61B5/154—Devices using pre-evacuated means
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- A—HUMAN NECESSITIES
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- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
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- A61P7/02—Antithrombotic agents; Anticoagulants; Platelet aggregation inhibitors
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5021—Test tubes specially adapted for centrifugation purposes
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
- B01L3/5082—Test tubes per se
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
Abstract
Disclosed herein is a container device for receiving blood in a reservoir and controlling coagulation in a blood sample, wherein the a reservoir comprises thrombin and coagulation controlling agent selected from citrate, isocitrate, a protamine salt, benzamidine, or para-aminobenzamidine. Additives such as water soluble polymers and sugars are also contemplated. The method combines thrombin and a coagulation controlling agent to stabilize thrombin or accelerate its activity in a blood sample. such as water soluble polymers and sugars are also contemplated. The method combines thrombin and a coagulation controlling agent to stabilize thrombin or accelerate its activity in a blood sample.
Description
COAGULATION CONTROLLING AGENTS AND DEVICES COMPRISING THE SAME
CROSS-REFERENCE TO RELATED APPLICATIONS
[ 0 0 0 1 ] This application claims the benefit of the filing
date of United States Provisional Patent Application No.
61/504,496 filed July 5, 2011, the disclosure of which is
hereby incorporated herein by reference .
BACKGROUND OF THE INVENTION
[ 0 0 0 2 ] Serum is the liquid portion of whole blood which
remains after blood is allowed to clot. Devoid from the serum
are the whole blood components which are consumed or entrapped
during the clotting process namely red blood cells, white
blood cells, platelets and the blood coagulation factors.
Thus, serum includes all of the proteins not used during
clotting and also includes sugars, fats, enzymes, antibodies,
antigens, hormones, charged particles (i.e., electrolytes) and
exogenous substances {e.g., drugs and microorganisms) . Serum
is, therefore, the preferential test substrate on which to
perform clinical tests used to diagnose and monitor muscle and
organ function, metabolic balances, and basic physiologic
functions . Also serum is preferred to perform other
analytical tests such as enzyme, electrolyte, protein, and
glucose assays since the interference of unwanted substances
has been removed through the clotting process.
[ 0 0 0 3 ] Serum is obtained by centrifugation of clotted
blood. In the past, the production of serum from whole blood
has been a passive process in which freshly collected blood is
added to a glass test tube and allowed to clot.
Alternatively, other serum tubes may contain silica or ellagic
acid to stimulate the coagulation cascade. Blood, once
removed from the body, has a natural tendency to clot and its
exposure to a surface such as glass promotes clotting in a
more efficient manner. Contact with a glass surface causes
the activation of coagulation factors which interact in a
mechanism commonly referred to as the coagulation cascade. In
this process, an inactive coagulation factor is chemically
converted to an active enzyme which subsequently converts yet
another inactive precursor. The end result of the coagulation
cascade is a conversion of the soluble plasma protein
fibrinogen, to an insoluble protein, fibrin, whereby the
fibrin clot entraps the white cells, red cells, and platelets
forming a solid gelatinous mass. Substances not consumed in
the process, such as those described above, remain free of the
gelatinous mass and are found in the liquid matrix, i.e. the
serum .
The passive clotting process described above causes
several problems. While blood from normal healthy individuals
may clot in 30 minutes or longer in a glass test tube, blood
from sick individuals who may have deficiencies of coagulation
proteins or from patients who are receiving anticoagulation
therapy {i .e., oral anticoagulants or heparin) may require
extensive time to clot (i .e., 2-8 hours). Consequently, there
has been a delay associated with the obtaining of blood
specimens and the performance of the analytical tests, thereby
affecting the ability of the clinician to quickly provide
optimal patient care. In addition, the blood from individuals
with deficiencies of coagulation proteins or patients
receiving anticoagulation therapy may never form a complete
and adequate fibrin mass. For example, incomplete clotting in
heparinized blood specimens results in a poor quality serum
substrate upon which to perform the chemical test.
Furthermore, serum from heparin anticoagulated blood, which
may not have clotted initially, may begin to clot once placed
in the analytical device, thereby clogging the system and
causing an instrument shutdown.
In order to improve the predictability and
uniformity of the clot forming process, technicians have
routinely added the clot promoting agent thrombin and/or a
closely related enzyme with "thrombin-like " activity
(e.g. batroxobin) to the blood specimen. Thrombin actively
converts fibrinogen to fibrin, thereby forming the clot more
efficiently then the slower glass-activated clotting process.
One drawback to using thrombin and/or another enzyme in a
blood collection tube is that these proteins are not as stable
as the silica-based contact pathway activators. For example,
exposure to moisture and elevated temperatures can inactivate
thrombin. However, silica-based methods have often failed to
give complete blood clotting resulting in a partially clotted
material that could interfere with the testing of blood
samples such as serum obtained from whole blood.
BRIEF SUMMARY OF THE INVENTION
Disclosed herein is a sterile and evacuated
container for collecting serum, comprising a first end and a
second end and at least one interior wall defining a reservoir
portion for receiving the blood, wherein the reservoir
comprises thrombin and at least one coagulation controlling
agent, and a closure piercable by a needle for supplying blood
to the reservoir.
In some embodiments described herein, the
coagulation controlling agent is a polycarboxylic acid having
a molecular weight of less than about 500g/mol. In some
embodiments, the polycarboxylic acid is citrate or isocitrate
("citrate")- further embodiments, the concentration of the
polycarboyxlic acid coagulation controlling agent ranges from
about 0.5 mM to about 100 mM of concentrated formulation. In
yet further embodiments, the concentration of the
polycarboxylic acid coagulation controlling agent ranges from
about 1 mM to about 50 mM of concentrated formulation.
In other embodiments, the coagulation controlling
agent is a protamine salt or a homolog or derivative thereof.
In further embodiments, the concentration of the protamine
coagulation controlling agent ranges from about 0.05 mg/mL to
about 5 mg/mL of blood sample. In yet further embodiments,
the concentration of the protamine coagulation controlling
agent ranges from about 0.25 mg/mL to about 0.5 mg/mL of blood
sample .
In other embodiments, the coagulation controlling
agent is a weak competitive inhibitor of thrombin. Weak
inhibition is reversible by subjecting the samples to
conditions that do not favor the inhibitor binding in a manner
that inhibits thrombin activity. In some embodiments, the
weak competitive inhibitor of thrombin is a small molecule
having a molecular weight of less than about 500g/mol. In
some embodiments, the weak competitive inhibitor of thrombin
has an inhibition constant of greater than about 0.1
micromolar. In some embodiments, the weak competitive
inhibitor of thrombin is a compound of Forumula (I) :
wherein R is selected from the group consisting of -
NR ; -N0 ; a substituted or unsubstituted, linear or branched
alkyl group having between 1 and 6 carbon atoms; a halogen;
-CO O R ; -CO- R ; -OH; or -ORi ;
R and R may be the same or different and may be
selected from the group consisting of hydrogen, halogen, a
substituted or unsubstituted, linear or branched Ci- Ci alkyl
group, and -(CH ) -aryl; and
x is an integer ranging from 1 to 10.
In a further exemplary embodiment, the coagulation
controlling agent is benzamidine. In another exemplary
embodiment, the coagulation controlling agent is para-
aminobenzamidine . In a further embodiment, a concentration of
the coagulation controlling agent of Formula (I) ranges from
about 0.5 mM to about 20 mM of concentrated formulation. In
yet a further embodiment, a concentration of the coagulation
controlling agent of Formula (I) ranges from about 1 mM to
about 10 mM of concentrated formulation.
In another embodiment, the container further
comprises a collection container that further comprises an
additive selected from the group consisting of a water-soluble
polymer and a sugar. In another embodiment, the water-soluble
polymer is selected from the group consisting of
polyvinylpyrrolidone, polysaccharides and polyethyleneglycol .
In a further embodiment, the sugar is selected from the group
consisting of dextran, trehalose, lactose, sucrose, glucose,
mannitol, and sorbitol.
In another embodiment, the container further
comprises a separating element. In a further embodiment, the
separating element comprises a mechanical separating element.
In another embodiment, the separating element comprises a gel
composition. In a further embodiment, the gel composition
comprises a thixotropic gel. In yet a further embodiment, the
gel composition is contained in a capsule.
In another embodiment, the container further
comprises collected blood.
Also disclosed herein is a method of collecting
blood comprising providing a container comprising thrombin and
a coagulation controlling agent in a concentration sufficient
to stabilize thrombin and/or accelerate its activity, and
adding to the container a blood sample.
Also disclosed herein is a method of collecting
blood comprising providing a container comprising thrombin and
a coagulation controlling agent in a concentration sufficient
to reduce the concentration of fibrin in a blood serum sample,
and adding to the container a blood sample.
Also disclosed herein is a method of collecting
blood comprising providing a container comprising thrombin and
a coagulation controlling agent in a concentration sufficient
to stabilize thrombin and adding to the container a blood
sample .
In some embodiments of the above-described methods,
the coagulation controlling agent is a polycarboxylic acid
having a molecular weight of less than about 500g/mol. In
some embodiments, of the above-described methods, the
polycarboxylic acid is a citrate. In some embodiments, a
concentration of the coagulation controlling agent ranges from
about 0.5 mM to about 100 mM of concentrated formulation.
In the above-described methods, the coagulation
controlling agent is a protamine salt or a homolog or
derivative thereof as previously described and present in the
concentrations previously described. In some embodiments, a
concentration of the coagulation controlling agent ranges from
about 0.05 mg/mL to about 5 mg/mL of blood sample.
In some embodiments, the coagulation controlling
agent is a weak competitive inhibitor of thrombin. In some
embodiments, the weak competitive inhibitor of thrombin is a
small molecule having a molecular weight of less than about
500g/mol. In some embodiments, the weak competitive inhibitor
of thrombin has an inhibition constant of greater than about
0.1 micromolar. In some embodiments, the weak competitive
inhibitor of thrombin a compound of previously described
Formula (I).
In some embodiments of the above described methods,
the coagulation controlling agent is para-aminobenzamidine as
described in the concentration previously described. In some
embodiments, a concentration of the coagulation controlling
agent of Formula (I) ranges from about 0.5 mM to about 20 mM
of concentrated formulation.
In some embodiments of the above-described methods,
the collection container further comprises an additive
selected from the previously described group consisting of a
water soluble polymer and a sugar .
Also disclosed herein is a method of collecting
blood comprising providing a container comprising thrombin and
the coagulation controlling agent in a concentration
sufficient to accelerate the activity of thrombin, and adding
to the container a blood sample. In some embodiments of the
above-described method, the coagulation controlling agent is
the above-described protamine salt or a homolog or derivative
thereof in the previously described concentrations.
In some embodiments of the above-described method,
the method further comprises providing at least one of a
polycarboxylic acid having a molecular weight of less than
about 500g/mol or a weak competitive inhibitor of thrombin in
the container. In some embodiments of the above-described
method, the method further comprises providing both a
polycarboxylic acid having a molecular weight of less than
about 500g/mol and a weak competitive inhibitor of thrombin in
the container. In some embodiments of the collecting methods,
the polycarboxylic acid is a citrate as previously defined in
the previously described concentration. In some embodiments,
the weak competitive inhibitor of thrombin is a small molecule
having a molecular weight of less than about 500g/mol. In
some embodiments, the weak competitive inhibitor of thrombin
has an inhibition constant of greater than about 0.1
micromolar. In some embodiments, the weak competitive
inhibitor of thrombin is the compound of previously described
Formula (I) . In some embodiments of the collecting methods,
the coagulation controlling agent is the above described para-
aminobenzamidine in the above described concentration.
In some embodiments, the collection container for
the controlling methods further comprises an additive selected
from the group consisting of a water soluble polymer and a
sugar .
Also disclosed herein is a method of collecting
blood comprising providing a container comprising thrombin and
a coagulation controlling agent in a concentration sufficient
to reduce the concentration of fibrin in a blood sample, and
adding to the container a blood sample.
In some embodiments of the method for collecting ,
the coagulation controlling agent is a polycarboxylic acid
having a molecular weight of less than about 500g/mol as
previously described in the concentration previously
described .
In some embodiments of the method for collecting,
the coagulation controlling agent is a protamine salt or a
homolog or derivative thereof or previously described in the
concentrations previously described.
In some embodiments of the method for collecting,
the coagulation controlling agent is a compound of above
described Formula (I) .
In some embodiments of the method for collecting,
the coagulation controlling agent is para-aminobenzamidine as
previously described in the above described concentrations.
In some embodiments of the method for collecting, the
collection container further comprises an additive selected
from the group consisting of a water soluble polymer and a
sugar .
BRIEF DESCRIPTION OF THE DRAWINGS
depicts a collection container suitable for
use in the current invention.
is a chart showing the effects of protamine
in mitigating fibrin mass formation.
is a chart showing the relationship between
protamine concentration and clotting time.
is a chart illustrating the effect
protamine on thrombin activity.
is a chart illustrating the effect of
protamine on thrombin activity.
is a chart illustrating the effect
p-aminobenzamidine on thrombin activity.
is a chart illustrating the effect of
p-aminobenzamidine on thrombin activity.
DETAILED DESCRIPTION
Described herein are methods, devices, and kits for
enhancing, promoting, stabilizing, accelerating, or
controlling the action of thrombin (hereinafter referred to
as "controlling coagulation"). By enhancing, promoting or
controlling the action of thrombin, it is meant that blood
would coagulate or clot more fully and/or more quickly than a
sample of blood alone or blood containing the addition of
thrombin or a thrombin-like substance (hereinafter
"thrombin"). More particularly, methods, and devices are
described for stabilizing, accelerating, or otherwise
controlling thrombin's ability to convert fibrinogen to fibrin
and/or otherwise reducing the amount of insoluble fibrin
and/or fibrinogen in a blood sample (e.g. serum) .
According to one embodiment described herein, is a
device having a container with (1) an amount of thrombin (or
thrombin-like substance), and (2) an amount of at least one
coagulation controlling agent therein. The thrombin and the
coagulation controlling agent are present for admixing with a
blood sample, preferably immediately on its collection.
However, any of the thrombin or the coagulation controlling
agent may be added to the sample after its collection.
As used herein, the term "blood sample" means any
biological sample containing at least some blood components
capable of clotting. In some embodiments, the blood sample is
whole blood. In other embodiments, the blood sample (e.g.
serum) is derived from a whole blood sample, whereby one or
more components of blood have already been removed by
centrifugation . In yet other embodiments, the blood sample is
a plasma concentrate.
The container can encompass any sample collection
device including tubes such as test tubes and centrifuge
tubes; closed system blood collection devices, such as
collection bags; syringes, especially pre-filled syringes;
catheters; micro-well and other multi-well plates; arrays;
tubing; laboratory vessels such as flasks, spinner flasks,
roller bottles, vials, microscope slides, microscope slide
assemblies, coverslips, films and porous substrates and
assemblies; pipettes and pipette tips, etc.; tissue and other
biological sample collection containers, including lancets,
capillary tubes, and Microtainer® brand products available
from Becton Dickinson; Vacutainer® brand venous blood
collection tubes available from Becton Dickinson including
those tubes prepackaged with thrombin; and any other container
suitable for holding a biological sample, as well as
containers and elements involved in transferring samples. The
coagulation controlling agent may be introduced into any of
these containers provided they meet the criteria outlined
herein .
The container may be comprised of (i.e. manufactured
from) plastic or glass, provided that it meets the criteria of
the invention. In some embodiments, the container is
comprised of polypropylene, polyethylene,
polyethyleneterephthalate, polystyrene, polycarbonate,
cellulosics, polytetraf luoroethylene , and other fluorinated
polymers may also be used. In other embodiments, the
container is comprised of polyolefins, polyamides, polyesters,
silicones, polyurethanes , epoxies, acrylics, polyacrylates ,
polysulf ones, polymethacrylates, PEEK, polyimide and
fluoropolymers such as PTFE Teflon®, FEP Teflon®, Tefzel®,
poly (vinylidene fluoride), PVDF and perfluoroalkoxy resins.
Glass products including silica glass are also used to
manufacture the container. One exemplary glass product is
PYREX® available from Corning Glass, Corning, N.Y. Ceramic
containers can be used according to embodiments of the
invention. Cellulosic products such as paper and reinforced
paper containers can also serve as container materials.
In accordance with one embodiment of the present
invention, the container is pre-treated with at least one of
thrombin or a coagulation controlling agent, and is packaged
in a ready-to-use form. In other embodiments, the container
is pre-treated with both thrombin and the coagulation
controlling agent. Typically, the packaged container is
sterile and is also packaged in sterile packaging materials.
The packaged collection (or "kit") may contain instructions
for use, storage, shipping, and/or handling.
The thrombin used as part of the present invention
may be from any source, including those of natural and
synthetic origins. In some embodiments, bovine thrombin is
used. In other embodiments, thrombin from other species is
used. In yet other embodiments, recombinant thrombin is used.
The recombinant thrombin may be modified such that it has
improved stability or is resistant to inactivation by other
blood components when compared with unmodified thrombin. In
preferred embodiments, thrombin is alpha-thrombin .
Alpha-thrombin is well known to those skilled in the art and
is described in detail herein. The concentration of thrombin
used in the container ranges from about 0.1 to about
100 Units (u) per milliliter of blood volume (u/ml) . In some
embodiments, the concentration of thrombin used in the
container ranges from about 1 to about 20 units per milliliter
of blood volume.
The container also comprises at least one
coagulation controlling agent. Without wishing to be bound by
any particular theory, it is believed that the coagulation
controlling agent is able to achieve at least one of the
following: (1) at least partially stabilize thrombin; (2) at
least partially reduce the amount of insoluble fibrin and/or
fibrinogen in a blood sample (e.g. serum); or (3) at least
partially accelerate the action of thrombin.
In one embodiment described herein, the coagulation
controlling agent is a protamine, or a salt or hydrate
thereof. Any protamine or derivative thereof may be used
provided it meets the criteria of the claimed invention
described above. In some embodiments, the protamine is
derived from salmon sperm and has the sequence identified in
SEQ. ID. NO. 1 . (SEQ. ID. NO. 1 : PRRRRSSSR PVRRRRRPRV
SRRRRRRGGR RRR) . Protamine sequences from other related
sources could additionally be considered, as they have very
similar sequences and may also have desirable properties.
Without wishing to be bound by any particular theory, it is
believed that a protamine may either stabilize thrombin or
accelerate its activity.
In one embodiment described herein, the coagulation
controlling agent is a weak active-site competitive (and
therefore reversible) inhibitor of thrombin. It is believed
that the addition of such an inhibitor stabilizes thrombin
during the shelf-life of the packaged combination of blood
collection device and thrombin-containing formulation.
Without wishing to be bound by any particular theory, it is
believed that the weak active-site competitive inhibitor
stabilizes thrombin by slowing its self-destructive autolytic
activity. When diluted with blood, it is believed that the
weak inhibitor dissociates from thrombin thereby allowing it
to become better dispersed in the sample prior to its
involvement in clot formation.
In some embodiments, the weak active-site
competitive inhibitor of thrombin is a small molecule having a
molecular weight of less than about 500g/mol. In some
embodiments, the weak competitive inhibitor of thrombin has an
inhibition constant (Ki) of greater than about 0.1 micromolar.
In other embodiments, the weak competitive inhibitor of
thrombin has an inhibition constant ranging from between about
0.1 micromolar to about 1000 micromolar. In some embodiments,
the weak competitive inhibitor of thrombin comprises the
compounds of Formula (I) .
In some embodiments, the weak active-site
competitive inhibitor is a substituted or unsubstituted
benzamidine, such as represented by the above-described
Formula (I).
In one embodiment, the compound of Formula (I) is
benzamidine. In yet other embodiments, the coagulation
controlling agent is selected from the group consisting of
para-aminobenzamidine , meta-aminobenzamidine, and
ortho-aminobenzamidine .
In another embodiment described herein, the
coagulation controlling agent is a low molecular weight
polycarboxylic acid compound (or a salt thereof), having a
molecular weight less than about 500g/mol. For example,
suitable polycarboxylic acid compounds may have the formula
2 2 3
C (0)OH-F^-R (C (0) OH) -R -C (0) OH, where R , R , and R may be the
same or different and may be a substituted or unsubstituted
aliphatic or aromatic group, and wherein any of R , R , or R
may contain any number of additional carboxylic acid groups .
In some embodiments, the polycarboxylic acid compound is
sodium citrate. In other embodiments, the polycarboxylic acid
compound is isocitrate.
Without wishing to be bound by any particular
theory, it is believed that citrate stabilizes alpha-thrombin
by reducing the rate at which it is cleaved and converted to
less active forms (e.g. beta-thrombin or gamma-thrombin) .
Moreover, and again without wishing to be bound by any
particular theory, it is believed that citrate is able to
stabilize alpha-thrombin comparatively better than
alpha-thrombin combined with one of TRIS, phosphate,
histidine, or HEPES buffers.
In one embodiment, the citrate coagulation
controlling agent is combined with an additional component
selected from the group consisting of water soluble polymers
and sugars. Exemplary water soluble polymers include
polyvinylpyrrolidone, polysaccharides, and polyethyleneglycol.
Exemplary sugars include dextran, cyclodextrins , trehalose,
lactose, sucrose, glucose, mannitol, and sorbitol. Indeed,
the additional component (s) could be selected from common
pharmaceutical excipients that are well known to those skilled
in the art of pharmaceutical formulation.
Without wishing to be bound by any particular
theory, it is believed that the water soluble polymer can act
as a binder to facilitate the spray application of the
formulation onto the surface of a blood collection device.
In other embodiments, the citrate coagulation
controlling agent is combined with a surfactant. Examples of
suitable surfactants include those belonging to the class of
compounds known as "siloxane alkoxylates ".
In some embodiments, the pH of the citrate solution
with thrombin ranges between about 5.5 to about 7.5.
In some embodiments, the citrate coagulation
controlling agent contains other salts, buffers, or proteins.
In other embodiments, the citrate solution is substantially
free of other salts, buffers, or proteins. In yet other
embodiments, the citrate solution is free of at least one of
albumin, sodium chloride, or TRIS-HCl.
Those skilled in the art will be able to select an
appropriate concentration of a coagulation controlling agent
suitable for the uses described herein based on the guidance
provided herein. Of course, different concentrations of
coagulation controlling agent may be needed depending on the
amount of thrombin provided in the sample tube, the type of
thrombin used, the method of applying the formulation to the
container, the volume of the blood sample and the type of
blood sample collected. Mixtures of different coagulation
controlling agents in varying concentrations may also be used
to provide the desired result. For example, thrombin may be
combined with both a protamine and a compound of Formula (I)
to provide the desired effect. Similarly, thrombin may be
combined with both a protamine and a polycarboxylic acid. As
such, the devices containing thrombin and the coagulation
controlling agent can be customized depending on sample type
and intended use.
In some embodiments where a protamine is added to a
sample containing thrombin, the protamine is present in a
concentration ranging from about 0.25 mg/mL to about 5 mg/mL
when dispersed in the blood sample. In other embodiments
where a protamine is added to a sample containing thrombin,
the protamine is present in a concentration ranging from about
0.25 mg/mL to about 0.5 mg/mL when dispersed in the blood
sample .
In some embodiments, the weak competitive inhibitor
of thrombin is present in a concentration ranging from about 1
to about 200 times the above-described inhibition constant
value. In some embodiments where a compound of Formula (I) is
added to a formulation containing thrombin, the compound of
Formula (I) is present in a concentration ranging from about
0.5 mM to about 20 mM in the formulation of liquid thrombin to
be applied (e.g. by spray) and dried on the surface of the
blood collection device. In other embodiments where a
compound of Formula (I) is added to a formulation containing
thrombin, the compound of Formula (I) is present in a
concentration ranging from about 1 mM to about 10 mM in the
formulation of liquid thrombin to be applied (e.g. by spray)
and dried on the surface of the blood collection device.
In some embodiments where citrate is added to a
formulation containing thrombin, alone or in combination with
an additional component, the citrate is present in a
concentration ranging from about 0.5 mM to about 100 mM of the
formulation of liquid thrombin to be applied (e.g. by spray)
and dried on the surface of the blood collection device. In
other embodiments where citrate is added to a formulation
containing thrombin, alone or in combination with an
additional component, the citrate is present in a
concentration ranging from about 1 mM to about 50 mM of the
formulation of liquid thrombin to be applied (e.g. by spray)
and dried on the surface of the blood collection device.
Any of the coagulation controlling agents may be
used alone or in combination with a buffering solution.
Buffering solutions are well known to those of ordinary skill
in the art and could easily be adapted for use in the present
invention .
The coagulation controlling agent, thrombin, and any
additives or buffers may be located on any surface of the
container. The thrombin and coagulation controlling agent may
be located on the same surfaces or on different surfaces, may
be intermixed, or may be separated from each other (for
convenience, any reference to the use of thrombin or the
coagulation controlling agent together should also be assumed
to include them apart) . The thrombin and/or coagulation
controlling agent may also be located on stoppers and seals
for closing such devices or on mechanical or other inserts
placed within such devices. The thrombin and/or coagulation
controlling agent is located anywhere along at least one
interior wall of the container or anywhere within the
reservoir portion. The location of the thrombin or
coagulation controlling agent are determined by several
variables, including the mode of application, the specific
coagulation controlling agent used, the internal volume and
internal pressure of the container, and the volume of the
biological sample drawn into the container.
The thrombin, coagulation controlling agent, and
additives or buffers, may be applied to the container by any
methods known in the art . The thrombin and coagulation
controlling agent may be applied by the same means or by
different means. Likewise, they may be applied together or
separately. For example, the thrombin and/or coagulation
controlling agent (s) may be sprayed onto the surface (as a
liquid, powder, or gel), spray dried, loosely dispensed or
lyophilized over the surface of the interior wall of the
container. In one embodiment, the thrombin and/or the
coagulation controlling agent is in a liquid solution and is
sprayed or otherwise placed into the container. Subsequently,
the solution may be lyophilized by methods that are known in
the art such as, for example, freeze drying. For example, by
freezing the solution and then slowly warming after freezing,
while simultaneously applying a vacuum, a freeze-dried powder
remains in the collection tube. An additive such as an
excipient, for example, PVP or trehalose, may also be added to
the thrombin and/or the coagulation controlling agent prior to
freeze drying so that the resulting stabilizing agent is
pelletized in the container. Alternatively, the thrombin
and/or the coagulation controlling agent, such as when in gel
or liquid form, for example, may be positioned in the
reservoir portion of the container. Typically, to dispose the
desired amount of thrombin and/or the coagulation controlling
agent into a container, one reconstitutes a solid form of the
thrombin and/or the coagulation controlling agent and then
dispenses the appropriate amount of liquid into the container.
The liquid may be spray dried, disposed into the bottom of the
container or subsequently lyophilized. In another aspect, the
thrombin and/or the coagulation controlling agent is formed
into a liquid or solid aerosol and sprayed onto one or more
surfaces of the interior of the container.
In some embodiments, the container is for drawing a
whole blood sample directly from a patient for coagulating a
blood sample immediately at the point of collection, and thus
used as a serum collection tube. The device may be an
evacuated system for collecting blood. Alternatively, the
device may be a partially-evacuated or a non-evacuated system
for collecting blood. A suitable example of an evacuated
system is a closed tube. A manual syringe draw is a suitable
example of both a partially-evacuated and a non-evacuated
system. Non-evacuated systems may also include automatic draw
systems .
shows a typical blood container 10, which
includes a container 12 defining an internal chamber 14. In
the embodiment illustrated, container 12 is a hollow tube
having a side wall 16, a closed bottom end 18 and an open top
end 20. Optionally, a separating member 13 is provided within
the container chamber 14. Separating member 13 serves to
assist in separating components of the sample, for example, by
centrifugation . Container 12 is dimensioned for collecting a
suitable volume of biological fluid, preferably blood. A
closure 22 for covering open end 20 to close container 12 is
preferred where a sterile product is demanded. For
conventional tubes, a screw cap is normally sufficient. For
evacuated collection tubes, a tight-fitting, elastomeric plug
is generally employed to contain the vacuum during the
required storage periods. Closure 22 forms a seal capable of
effectively closing container 12 and retaining a biological
sample in chamber 14. Closure 22 may be one of a variety of
forms including, but not limited to, rubber closures,
HEMOGARD® closures, metallic seals, metal-banded rubber seals
and seals of different polymers and designs. A protective
shield 24 may overlie closure 22. Container 12 also contains
thrombin and/or the coagulation controlling agent in
accordance with the present invention. The container may
contain one or both of the thrombin and/or coagulation
controlling agent. Of course, the other of the thrombin
and/or coagulation controlling agent may be added prior to
sample collection or after sample collection.
Container 12 can be made of glass, plastic or other
suitable materials described herein. Plastic materials can be
oxygen impermeable materials or may contain an oxygen
impermeable or semi-permeable layer. Alternatively,
container 12 can be made of a water and air permeable plastic
material .
The pressure in chamber 14 is selected to draw a
predetermined volume of biological sample into chamber 14.
Closure 22 is such that it can be pierced by a needle 26 or
other cannula to introduce a biological sample into container
12 as known in the art. Preferably, closure 22 is resealable.
Suitable materials for closure 22 include, for example,
silicone rubber, natural rubber, halobutyl rubber, styrene
butadiene rubber, ethylene-propylene copolymers and
polychloroprene .
Suitable examples of container 12 include
single-wall and multi-layer tubes . A more specific example of
a suitable container 12 is disclosed in United States Patent
No. 5,860,937 to Cohen, which is hereby incorporated by
reference in its entirety.
By way of example, one useful process for making the
devices according to the present invention involves obtaining
a container; adding thrombin and/or the coagulation
controlling agent to the container; drying or lyophilizing the
thrombin and/or the coagulation controlling agent; evacuating
the container; and sterilizing the container. The thrombin
and/or the coagulation controlling agent may be dispensed into
the container in solution form. Before or after adding the
thrombin and/or the coagulation controlling agent to the
collection container, a separating member may be added to the
container, if desired. An example of a suitable
lyophilization/evacuation process is as follows: the container
having thrombin and/or the controlling agent disposed therein
is frozen at a temperature of about -40°C at a pressure of
about 760mm for about 6 to 8 hours; the container is dried as
the temperature is ramped from -40°C to about 25°C, at a
pressure of about 0.05mm, for about 8 to 10 hours; and the
container is then evacuated at a temperature of about 25°C at
a pressure of about 120mm for about 0.1 hours. In some
embodiments, the sterilization technique deploys radiation
with cobalt 60 radiation.
[ 0 0 7 3 ] In some embodiments, a sample collection tube having
a separating member 13 (e.g., a mechanical separating element,
gel, or other separating member including filter paper and the
like) for separating blood components is used. In such
aspect, the interior of the tube and/or the exterior of the
separating member may be treated with the thrombin and/or the
coagulation controlling agent. In such cases, the thrombin
and/or the coagulation controlling agent may be spray dried
and/or lyophilized on an exterior surface of the separation
media .
[ 0 0 7 4 ] Container 12 may also be a container for blood serum
collection or preparation. Such a container comprises, in
addition to the thrombin and/or coagulation controlling agent,
an element for separating serum from human or animal whole
blood. The element for separating serum from whole blood may
be a separating member such as a gel formulation or a
mechanical media. In some embodiments, the gel is a
thixotropic polymeric gel formulation. The gel may be a
homopolymer or a copolymer and may include polyester,
polyacrylic, or silicone-based gels.
Other commercially available blood collection tubes
suitable for use herein include the following, all of which
are sold by Becton, Dickinson and Company, Franklin Lakes,
N.J., with all registrations and trademarks belonging to
Becton, Dickinson and Company: VACUTAINER® brand SST™ tubes,
catalog nos . including but not limited to 367983, 367977, and
367986; VACUTAINER® brand Serum tubes, catalog nos. including
but not limited to 367812, and 367815, VACUTAINER® brand
Thrombin tubes, catalog nos. including but not limited to
367755 and 366525, VACUTAINER® brand RST tubes, catalog nos.
including but not limited to 368771 or 368774 and any of the
MICROTAINER® brand serum and serum separator tubes. As noted
above, any suitable collection device, either evacuated or
non-evacuated, is contemplated for us with the coagulation
agents described herein. Of course, those skilled in the art
may modify any container in accordance with the present
invention to render it suitable for use.
According to another embodiment, a kit having at
least two containers comprising thrombin and the coagulation
controlling agent described herein. For example, the kit has
a primary collection tube, e.g., a plasma separating tube
having a separating element therein, and a secondary tube for
testing, e.g., for pouring or otherwise dispensing the
collected plasma into the primary collection tube.
Optionally, the kit could include a tube-to-tube transfer
device to prevent the need for pouring or other unsafe
transfer practices, in which case the secondary tube would be
at a reduced pressure to draw in the plasma. One using such a
kit would collect a sample in the primary tube, coagulate the
blood components with or without centrifugation, transfer the
sample of interest to the secondary testing tube, and perform
the testing. The secondary testing tube could be of a variety
of sizes, depending on the desired testing. The kit could
also include packaging and/or instructions for use.
In one embodiment, the kit for collecting and
storing the biological sample has a primary sterile and
evacuated container, wherein the primary container has
thrombin and at least one coagulation controlling agent
therein and a closure piercable by a needle; and a secondary
container, wherein the secondary container also has a
coagulation controlling agent .
According to another embodiment described herein is
a method for promoting or enhancing the coagulation or
clotting of blood components comprising providing a container
comprising a thrombin and a coagulation controlling agent in a
concentration sufficient (i) to stabilize and/or accelerate
the activity of thrombin, or (ii) reduce the amount of soluble
fibrin in the sample; and adding to the container a whole
blood sample.
Exemplary methods include obtaining a blood sample
and introducing the sample into the container comprising
thrombin and coagulation controlling agent. In some
embodiments, the blood sample is withdrawn from the patient
directly into the container without any intervening process
steps . Although not being held to a particular theory of
operation, it is believed that collecting the biological
sample directly from the patient, such as when collecting a
whole blood sample, and introducing the sample directly into a
container already comprising (i.e. pretreated with the agent)
the thrombin and the coagulation controlling agent,
substantially assists in stabilizing or accelerating or
otherwise controlling the action of thrombin or reducing the
amount of soluble fibrin present in the sample. The described
methods are useful with any of the containers, additives and
additional anti-coagulants disclosed herein.
Examples
Example 1 : Mitigating Effect of Protamine on Fibrin
Masses in Serum Samples
Protamine sulfate was first resuspended in about
0.5 M hydrochloric acid at a concentration of about 250 mg/mL.
This solution was then titrated to a pH of about 6 with
N sodium hydroxide. This titration to about pH 6 was
performed immediately since prolonged exposure of protamine to
low pH, it was believed, would result in hydrolysis and loss
of activity. It is also believed that more dilute solutions
of hydrochloric acid could be used for preparing the protamine
solution .
The protamine solution was then mixed in a ratio of
about 1:1 with a solution of about 1250 U/mL of thrombin in
about 40 mM sodium citrate at about pH 6.0, containing about
mg/mL of a siloxane alkoxylate surfactant, and 2 mg/mL
polyvinylpyrrolidone (PVP) .
The resulting reagent was filtered and sprayed in
shots into 13x100 mm polyethyleneterephthalate (PET)
tubes. The tubes were then dried by forced hot air, and
evacuated and stoppered.
Tubes were filled with freshly drawn human blood and
allowed to clot for between about 1 to about 3.5 minutes
(randomized) and then centrifuged. The resulting serum was
scored by visual assessment for the presence of a "fibrin
mass" in the serum compartment on a scale of 0 to 3, with 3
being the most severe. The results for "fibrin masses" were
demonstrated, as in . The absence of a fibrin mass
(rating of "zero") indicated that the clotting reaction was
rapid and complete, with all fibrin (ogen) precipitating before
the centr ifugation step.
As shown in the protamine at a concentration
of about 0.5 mg/mL improved the performance of the tube
dramatically. With the addition of protamine, only 1 tube
in 8 had a visible fibrin mass with either about 5 U/mL or
about 10 U/mL thrombin. This was in contrast to the tubes
without protamine where 7 of 8 had visible masses with about
U/mL thrombin, and 6 of 8 had visible masses with about
U/mL thrombin. Accordingly, it is believed that the
addition of a protamine coagulation controlling agent to a
blood sample container that also contains thrombin mitigated
the tendency of such masses (attributable to incomplete action
of thrombin on fibrinogen before centrifugation ) to form.
[ 0 0 8 7 ] Example 2 : Protamine Accelerates Clotting Action of
Thrombin
[ 0 0 8 8 ] Solutions of thrombin and protamine in about 20 mM
of citrate at a pH of about 6 were mixed in a ratio of about
1:1 with reconstituted commercial lyophilized human plasma and
tested on a Stago Compact coagulation analyzer. The resulting
clotting times were measured as a function of thrombin and
protamine concentration for a given thrombin concentration.
The concentrations listed on the graph of represent the
thrombin/protamine solutions before being mixed with the
plasma .
[ 0 0 8 9 ] The results showed a decreasing clotting time with
increasing protamine concentration. As such, it is believed
that the addition of a protamine enhances the clotting
activity of thrombin. Without wishing to be bound by any
particular theory, it is posited that the protamine
accelerated the clotting time by (1) directly accelerating the
enzymatic action of thrombin; (2) preventing thrombin
inactivation by endogenous inhibitors in the plasma;
(3) accelerating the precipitation of fibrin or some
combination of these effects.
[ 0 0 9 0 ] Example 3 : Accelerated Stability Results with
Thrombin and Protamine
[ 0 0 9 1 ] A mixture of thrombin (about 1250 U/mL), protamine
(about 125 mg/mL or about 62.5 mg/mL), and a siloxane
alkoxylate surfactant was prepared in about 20 mM citrate at a
pH of about 6.0. About 20 of this mixture was sprayed into
13x100 mm PET tubes. The tubes were dried by forced hot air,
evacuated, and stored in the refrigerator. At the initiation
of the study, the tubes were uncapped and placed in a
controlled environmental chamber previously equilibrated to
about 50°C and about 45% relative humidity. At various time
intervals, tubes were removed and evaluated for thrombin
activity on the Stago Compact coagulation analyzer. The
results are presented in . The error bars indicate the
95% confidence interval for the thrombin activity with 5
replicates. clearly illustrates that thrombin activity
over time was better with the protamine than without it.
When the thrombin activity of subsequent time points
were normalized on the initial value, the resulting curves
could be used to compare the relative stability of each
formulation (see ). From the FIGs. it is observed that
the blood samples with formulations containing protamine
sulfate were more stable than blood samples combined with
thrombin formulations without protamine.
Example 4 : Accelerated Stability Results with
Thrombin and para-Aminobenzamidine
The coagulation controlling agent
para-aminobenzamidine was evaluated to determine if it
improved the stability of sprayed thrombin under accelerated
stability conditions. Without wishing to be bound by any
particular theory, it is believed that para-aminobenzamidine
stabilizes the spray-dried thrombin under conditions of
extremely high humidity, where thrombin autolytic degradation
can be rapid, even at room temperature .
Thrombin was prepared in about 5 mM citrate or
isocitrate at a pH of about 6.1. Para-aminobenzamidine was
added at a concentration of about 3 mM. About 16 of this
solution was sprayed into 13x100 mm PET tubes. The tubes were
dried by forced hot air, and evacuated. Sealed tubes were
then placed in a controlled environmental chamber previously
equilibrated to about 43°C and about 75% relative humidity.
At various time intervals, the tubes were removed and
evaluated for thrombin activity on the Stago Compact
coagulation analyzer. The results are presented in and
. The error bars indicate the 95% confidence interval
for the thrombin activity with 5 replicates. The data in the
figures indicates that the coagulation controlling agent
para-aminobenzamidine preserves thrombin stability over time
(compared to citrates alone) in the packaged product, before
use .
Although the invention herein has been described
with reference to particular embodiments, it is to be
understood that these embodiments are merely illustrative of
the principles and applications of the present invention. It
is therefore to be understood that numerous modifications may
be made to the illustrative embodiments and that other
arrangements may be devised without departing from the spirit
and scope of the present invention as defined by the appended
claims .
Claims (18)
1. A container for collecting blood, comprising a first end and a second end and at least one interior wall defining a reservoir portion for receiving the blood, wherein at least a surface of said reservoir is pre-disposed with a mixture comprising thrombin and a polycarboxylic acid compound having a molecular weight of less than about 500g/mol.
2. The container of claim 1, wherein said polycarboxylic acid compound is selected from the group consisting of citrate or isocitrate.
3. The container of claim 1, wherein a concentration of said polycarboxylic acid compound ranges from about 0.5 mM to about 100 mM of concentrated formulation.
4. The container of claim 3, wherein a concentration of said polycarboxylic acid compound ranges from about 1 mM to about 50 mM of concentrated formulation.
5. The container of claim 1, wherein said collection container further comprises an additive selected from the group consisting of a water-soluble polymer and a sugar.
6. The container of claim 5, wherein said water-soluble polymer is selected from the group consisting of polyvinylpyrrolidone, polysaccharides, and polyethyleneglycol.
7. The container of claim 5 or claim 6, wherein said sugar is selected from the group consisting of dextran, cyclodextrins, trehalose, lactose, sucrose, glucose, mannitol, and sorbitol.
8. The container of any one of the preceding claims, further comprising a separating element.
9. The container of claim 8, wherein said separating element comprises a gel composition.
10. The container of claim 9, wherein said gel composition comprises a thixotropic gel.
11. The container of claim 9 or claim 10, wherein said gel composition is contained in a capsule.
12. The container of any one of claims 8 to 11, wherein said separating element comprises a mechanical separating element.
13. The container of any one of the preceding claims, wherein the closure is pierceable by a needle and wherein the container further comprises blood introduced into the container through a needle.
14. A method of collecting blood comprising providing a container, wherein at least a surface of the container is pre- disposed with a mixture comprising thrombin and polycarboxylic acid compound having a molecular weight of less than about 500g/mol in a concentration sufficient to stabilize thrombin, and adding to the container a blood sample.
15. The container of claim 14, wherein said polycarboxylic acid compound is selected from the group consisting of citrate or isocitrate.
16. The method of claim 14 or claim 15, wherein a concentration of said polycarboxylic acid compound ranges from about 0.5 mM to about 100 mM of concentrated formulation.
17. The method of any one of claims 14 to 16, wherein said container further comprises an additive selected from the group consisting of a water-soluble polymer and a sugar.
18. The method of any one of claims 14 to 17, wherein said method further comprises combining an additive selected from the group consisting of a water-soluble polymer and a sugar in said container.
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US201161504496P | 2011-07-05 | 2011-07-05 | |
US61/504,496 | 2011-07-05 | ||
PCT/US2012/045243 WO2013006550A1 (en) | 2011-07-05 | 2012-07-02 | Coagulation controlling agents and devices comprising the same |
Publications (2)
Publication Number | Publication Date |
---|---|
NZ619674A NZ619674A (en) | 2015-06-26 |
NZ619674B2 true NZ619674B2 (en) | 2015-09-29 |
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