NZ614718B2 - Use of phospho-akt as a biomarker of drug response - Google Patents

Abstract

Use of phospho-Akt as a biomarker for predicting the response, such as resistance, to compounds, wherein phospho-Akt is Akt that has been phosphorylated on one or more residues, with the proviso that for Akt1, Akt2, and Akt3 the designation phospho-Akt is used to indicate phosphorylation at a site other than T308, T309 or T305 respectively, wherein the compounds are microtubule destabilizing agents of general formula (I), wherein the substituents are as described in the specification. Methods of treatment of neoplastic and autoimmune diseases with these compounds are also disclosed. ther than T308, T309 or T305 respectively, wherein the compounds are microtubule destabilizing agents of general formula (I), wherein the substituents are as described in the specification. Methods of treatment of neoplastic and autoimmune diseases with these compounds are also disclosed.

Description

Use of phospho-Akt as a biomarker of drug response The present invention generally relates to use of phospho-Akt as a biomarker for predicting the response of a disease, such as a neoplastic or mune disease, preferably cancer, to a compound of general formula I, such as 3-(4-{1-[2-(4-amino-phenyl)oxo-ethyl]-1H-benzoimidazolyl}-furazanylamino)- propionitrile (BAL27862). In other aspects it relates to methods and kits involving the use of the biomarker. Also described are methods of treatment involving the use of the biomarker.

Microtubules are one of the components of the cell cytoskeleton and are composed of heterodimers of alpha- and beta- tubulin. Agents that target microtubules are among the most effective xic chemotherapeutic agents having a broad spectrum of activity. Microtubule destabilising agents (e.g. the vincaalkaloids such as vincristine, vinblastine and vinorelbine) are used for e in the treatment of several types of hematologic malignancies, such as lymphoblastic leukaemia and ma, as well as solid tumours, such as lung cancer. Microtubule stabilising agents (e.g. the taxanes such as paclitaxel, docetaxel) are used for example in the treatment of solid tumours, including breast, lung and te cancer.

However resistance to these known microtubule targeting agents can occur.

The resistance can either be nt or can be acquired after re to these agents. Such resistance therefore impacts patient survival rates, as well as choices of treatment regimes. Several potential mechanisms of resistance have been identified, and include defects in the microtubule targets, such as ed levels of beta-tubulin subtype III and acquired ons in beta-tubulin subtype I that are known to reduce taxane binding. Furthermore, defects in other cell proteins have been suggested to be ated with resistance to certain microtubule targeting agents, such as overexpression of the efflux pump oprotein (P-gp, also known as multi-drug resistance protein 1 or MDR1). Such factors may then be used as biomarkers of resistance to these conventional microtubule targeting agents.

A relatively recently discovered class of microtubule ilising agents are compounds encompassed by the formula given below: R3 R2 N R4 N R5 N N wherein R represents phenyl, thienyl or pyridinyl wherein phenyl is optionally substituted by one or two substituents independently selected from alkyl, halo-lower alkyl, hydroxy-lower alkyl, lower alkoxy-lower alkyl, acyloxy-lower alkyl, phenyl, hydroxy, lower alkoxy, hydroxy-lower alkoxy, lower -lower alkoxy, phenyl-lower alkoxy, lower alkylcarbonyloxy, amino, kylamino, dialkylamino, lower alkoxycarbonylamino, lower alkylcarbonylamino, substituted amino wherein the two substituents on nitrogen form together with the en heterocyclyl, lower alkylcarbonyl, carboxy, lower alkoxycarbonyl, cyano, halogen, and nitro; and wherein two adjacent substituents are methylenedioxy; and wherein pyridinyl is optionally substituted by lower alkoxy, amino or halogen; X represents a group C=Y, wherein Y stands for oxygen or nitrogen substituted by y or lower ; R1 represents en, lower alkylcarbonyl, hydroxy-lower alkyl or cyano-lower alkyl; R2, R3 and R6 represent hydrogen; R4 and R5, independently of each other, represent hydrogen, lower alkyl or lower alkoxy; or R4 and R5 together represent methylenedioxy; and pharmaceutically acceptable salts thereof; or wherein R ents phenyl or pyridinyl wherein phenyl is optionally substituted by one or two tuents independently selected from alkyl, halo-lower alkyl, hydroxy-lower alkyl, lower -lower alkyl, acyloxy-lower alkyl, phenyl, hydroxy, lower alkoxy, hydroxy-lower alkoxy, lower alkoxy-lower alkoxy, phenyl-lower alkoxy, lower alkylcarbonyloxy, amino, monoalkylamino, dialkylamino, lower alkoxycarbonylamino, lower alkylcarbonylamino, substituted amino wherein the two substituents on nitrogen form together with the nitrogen heterocyclyl, lower alkylcarbonyl, carboxy, lower alkoxycarbonyl, formyl, cyano, halogen, and nitro; and wherein two adjacent substituents are methylenedioxy; and wherein pyridinyl is ally substituted by lower alkoxy, amino or halogen; X represents oxygen; R1 represents hydrogen, lower alkylcarbonyl, hydroxy-lower alkyl or cyano-lower alkyl; R2, R3 and R6 represent hydrogen; R4 and R5, independently of each other, represent hydrogen, lower alkyl or lower or R4 and R5 together represent methylenedioxy; and pharmaceutically acceptable salts thereof; and wherein the prefix lower denotes a radical having up to and including a maximum of 7, especially up to and including a m of 4 carbon atoms.

These compounds are sed in /103994 A1, which is incorporated by cross-reference herein. These compounds have been shown to arrest tumour cell proliferation and induce apoptosis.

The synthesis of compounds of formula I is described in /103994 A1, in general on pages 29-35, and specifically on pages 39-55, which are incorporated herein by reference. They may be prepared as disclosed or by an analogous method to the processes described therein.

One compound falling within this class, known as BAL27862, and shown in WO2004/103994 A1 as example 58, and specifically incorporated by reference herein, has the structure and chemical name given below: N N N N O Chemical name: 3-(4-{1-[2-(4-Amino-phenyl)oxo-ethyl]-1H-benzoimidazol- furazanylamino)-propionitrile; or herein as Compound A.

Further compounds exemplified in WO2004/103994 A1 as examples 50 and 79 respectively, and also specifically incorporated by cross-reference , have the structures and chemical names given below: N N N N O Chemical name: 2-[2-(4-Amino-furazanyl)-benzoimidazolyl](4-amino-phenyl)- ethanone; or herein as Compound B N N N N O Chemical name: 1-[2-(6-Amino-pyridinyl)oxo-ethyl]-1H- midazolyl}-furazanylamino)-propionitrile; or herein as Compound C.

BAL27862 has activity across a broad panel of experimental, solid tumour xenograft models. Moreover, activity is retained even against tumour models which are selected for resistance to conventional microtubule ing agents (including the vinca-alkaloid microtubule ilisers and the microtubule stabilisers paclitaxel and epothilone B). BAL27862 activity is not affected by over-expression of the P-gp pump in any models tested in vitro, nor in human mammary tumour xenografts.

Additionally, BAL27862 retained its activity despite elevated levels of beta-tubulin subtype III and mutations in tubulin subtype I.

Hence, BAL27862 activity is not affected by a number of factors that confer ance to conventional microtubule targeting .

Moreover, it is known that compounds of general formula I have a different effect on the phenotype of cells compared to other ubule targeting agents, ing other microtubule destabilisers. Treatment with a compound of general formula I induces a consistent microtubule phenotype in tumour cell lines derived from a variety of organs, for example lung, cervix and breast, as seen in Figure 1.

Staining the microtubules in these cells with an anti-alpha-tubulin antibody shows that rather than the mitotic spindle fibres of untreated cells, only dot-like structures are visible in the treated cells. This same effect is also shown using Compounds C and B in Figures 2A and 2B respectively on the lung cancer cell line A549. It is however very ct from that observed with the conventional microtubule targeting agents vinblastine, colchicine, paclitaxel and zole as seen in Figures 3B, 3C, 3D and 4, respectively. The microtubules were stained with an anti-alpha-tubulin antibody and the cells viewed at a 1000 x magnification (Figures 3, 4). For the cells treated with BAL27862, multiple ke structures are visible, whereas, in stark st, the other conventional drugs produce filamentous ubule structures, or dense microtubule aggregate structures. These differences at the phenotypic level, at compound doses considered optimal in terms of antiproliferative , indicate a difference in the mode of action at the lar level.

Furthermore, it is known that BAL27862 elicits a dominant microtubule phenotype in the presence of the other microtubule ing agents. Treatment with vinblastine, colchicine, paclitaxel or nocodazole alone induced the microtubule phenotypes teristic of these agents (Figure 5A, 5D, 5G, 6C-6F respectively).

However, combination ent with BAL27862 for the last 4 hours resulted in disruption of these phenotypes; despite the continued presence of vinblastine, colchicine, paclitaxel or nocodazole (Figure 5B, 5E, 5H, 6G-6J respectively). In st, treating first with BAL27862 and subsequently for 4 hours in combination with vinblastine, colchicine, paclitaxel or nocodazole had no impact on generation of the phenotype consistent with BAL27862 treatment (Figure 5C, 5F, 5I, 6K-6N respectively).

These data all demonstrate that BAL27862 affects microtubule biology in a ent manner than conventional microtubule targeting agents.

Thus, from ation about conventional microtubule targeting agents, predictions cannot be made concerning if, or how, particular genes are involved in the action of compounds of general formula I.

An object of the present invention is to identify factors which are associated with se to compounds of formula I or pharmaceutically acceptable derivatives thereof, for example to identify s associated with resistance to compounds of general a I, in particular BAL27862 or pharmaceutically acceptable tives thereof, as defined below; and/or to provide the public with a useful choice.

It has surprisingly been found that phospho-Akt may be used as a biomarker of response to treatment with a compound of general formula I or pharmaceutically acceptable derivatives thereof, as defined below.

Accordingly, in a first aspect, the invention s to use of phospho-Akt as a ker for predicting the response to a compound, wherein phospho-Akt is Akt that has been phosphorylated on one or more residues, with the proviso that for Akt1, Akt2, and Akt3 the designation phospho-Akt is used to indicate phosphorylation at a site other than T308, T309 or T305 respectively, wherein the nd is a compound of general formula I, R3 R2 N R4 N R5 N N R represents phenyl, thienyl or pyridinyl wherein phenyl is ally substituted by one or two substituents independently selected from alkyl, halo-lower alkyl, hydroxy-lower alkyl, lower alkoxy-lower alkyl, acyloxy-lower alkyl, phenyl, hydroxy, lower alkoxy, hydroxy-lower alkoxy, lower alkoxy-lower alkoxy, phenyl-lower alkoxy, lower alkylcarbonyloxy, amino, monoalkylamino, dialkylamino, lower alkoxycarbonylamino, lower alkylcarbonylamino, substituted amino wherein the two substituents on nitrogen form together with the nitrogen heterocyclyl, lower alkylcarbonyl, carboxy, lower alkoxycarbonyl, cyano, halogen, and nitro; and n two adjacent substituents are methylenedioxy; and wherein pyridinyl is optionally substituted by lower alkoxy, amino or halogen; X represents a group C=Y, wherein Y stands for oxygen or nitrogen substituted by hydroxy or lower alkoxy; R1 represents en, lower alkylcarbonyl, hydroxy-lower alkyl or cyano-lower alkyl; R2, R3 and R6 represent hydrogen; R4 and R5, independently of each other, represent en, lower alkyl or lower alkoxy; or R4 and R5 together represent methylenedioxy; and pharmaceutically acceptable salts, solvates, esters and amides of naturally occurring amino acids, small peptides or pegylated hydroxy acids, and salts of such esters and amides thereof; or n R represents phenyl or pyridinyl wherein phenyl is ally substituted by one or two substituents independently ed from alkyl, halo-lower alkyl, hydroxy-lower alkyl, lower alkoxy-lower alkyl, acyloxy-lower alkyl, phenyl, hydroxy, lower alkoxy, hydroxy-lower alkoxy, lower alkoxy-lower alkoxy, phenyl-lower alkoxy, lower alkylcarbonyloxy, amino, monoalkylamino, dialkylamino, lower alkoxycarbonylamino, lower arbonylamino, substituted amino n the two substituents on nitrogen form together with the en heterocyclyl, lower alkylcarbonyl, carboxy, lower alkoxycarbonyl, formyl, cyano, halogen, and nitro; and wherein two adjacent substituents are methylenedioxy; and wherein pyridinyl is optionally substituted by lower alkoxy, amino or halogen; X represents oxygen; R1 represents hydrogen, lower alkylcarbonyl, hydroxy-lower alkyl or cyano-lower alkyl; R2, R3 and R6 represent hydrogen; R4 and R5, independently of each other, represent hydrogen, lower alkyl or lower alkoxy; or R4 and R5 together ent methylenedioxy; and ceutically acceptable salts, solvates, esters and amides of naturally occurring amino acids, small peptides or pegylated hydroxy acids, and salts of such esters and amides thereof, and n the prefix lower denotes a radical having up to and including a maximum of 7 carbon atoms, and wherein the response is of a disease in a subject and the biomarker phospho-Akt is measured ex vivo in a sample or samples taken from the human or animal body.

In a second aspect, the invention provides a method for predicting in a subject suffering from cancer the response of a disease that cancer to a compound of general formula I or a pharmaceutically acceptable salt, solvate, ester or amide of a naturally occurring amino acid, small peptide or pegylated hydroxy acid, or a salt of such ester or amide thereof as defined in a first aspect of the invention, comprising the steps of: a) measuring ex vivo a level of phospho-Akt, as defined in a first aspect of the invention, in a sample pre-obtained from tumour tissue or circulating tumour cells of the subject to obtain a value or values representing this level; and b) ing the value or values from step a) to a standard value or set of standard values from subjects with the same cancer type, n a higher level of o-Akt in the sample relative to the standard value or set of rd value is predictive of resistance of the subject's cancer to the compound of formula (I) or pharmaceutically acceptable salt, solvate, ester or amide of a naturally occurring amino acid, small peptide or pegylated hydroxy acid, or a salt of such ester or amide thereof.

In a third aspect, the invention relates to the use of a compound of general formula I or of a pharmaceutically acceptable salt, e, ester or amide of a naturally occurring amino acid, small peptide or pegylated hydroxy acid, or a salt of such ester or amide thereof as defined in a first aspect of the invention, for the preparation of a ceutical composition for treating a cancer in a subject in need thereof, wherein the subject is selected for treatment with the compound of general formula I or with the ceutically able salt, solvate, ester or amide of a naturally occurring amino acid, small e or pegylated hydroxy acid, or a salt of such ester or amide thereof as defined in a first aspect of the invention, if the level of the phospho-Akt defined in a first aspect of the invention, measured ex vivo in a sample taken from the subject, is not higher than a standard value or set of standard values from subjects with the same tumour histotype or from normal cells, tissue or body fluid.

In a fourth aspect the ion provides a kit when used for predicting the response to a compound of general formula I or of a pharmaceutically acceptable salt, solvate, ester or amide of a naturally occurring amino acid, small peptide or pegylated hydroxy acid, or a salt of such ester or amide thereof, as defined in a first aspect of the invention , comprising reagents necessary for measuring the level of the o-Akt, in a sample taken from a subject with a cancer, and further comprising a ator module which comprises a standard value or set of standard values of a level of the phospho-Akt defined a first aspect of the invention taken from samples of tumour tissue or circulating tumour cells of subjects with a cancer of the same histotype to which the level of the phospho-Akt in the sample is wherein said reagents comprise a capture reagent comprising a detector for o-Akt as defined in a first aspect of the invention and a detector reagent, and wherein the kit comprises a compound of the ing formula N N N N O or a pharmaceutically acceptable salt thereof. n statements that appear below are broader than what appears in the statements of the invention above. These ents are provided in the interests of providing the reader with a better understanding of the invention and its practice. The reader is directed to the accompanying claim set which defines the scope of the invention.

In one preferred embodiment of the invention, relatively high phospho-Akt levels in a tumour sample are associated with inherent resistance to BAL27862.

To date, the Akt family consists of three known genes, also ed to as isoforms, Akt1, Akt2 and Akt3. These genes are homologous at the nucleic acid level, as well as at the polypeptide sequence level. The Akt genes are also known by a y of alternative names, reflecting their discovery by different groups. The name Akt arose from its discovery as the human homologue of the proto-oncogene of the transforming irus AKT8. Akt is also known as n kinase B, or RAC (Related to A and C kinases), since these Akt proteins are closely related to protein kinase A (PKA) and protein kinase C (PKC). Thus, the Akt family is known by the following synonyms c-AKT; proto-oncogene c-Akt; protein kinase B; PKB; RAC; RAC serine/threonine-protein kinase; rac protein kinase and RAC-PK. Akt1, the gene first discovered, is also known as c-AKT1; PKB; PKB-alpha; PRKBA; RAC; RAC-alpha serine/threonine-protein kinase; RAC-ALPHA; RAC-PK-alpha and MGC99656. Akt2 is also known as protein kinase Akt-2; protein kinase B beta; PKBBETA; PRKBB; RAC-BETA; RAC-beta serine/threonine-protein kinase and RAC-PK-beta. Akt3 is also known as PKB-gamma; PKBG; PRKBG; RAC-gamma serine/threonine-protein kinase; RAC-gamma; RAC-PK-gamma; DKFZP434N0250 and STK-2. Alternative splice transcript variants have also been found for some of these genes. Alternative splice transcript variants encoding distinct ms have been described for Akt3, namely RAC-gamma serine/threonine-protein kinase isoform 1 and RAC-gamma serine/threonine-protein kinase isoform 2, which differ in length. The ation Akt shall be used herein to encompass the three d proteins Akt1, Akt2, Akt3 and all the synonyms listed above, including isoforms.

Akt may be post-translationally modified, including by phosphorylation at one or more sites. For example Akt1 is known to be able to be phosphorylated on Ser- 124, Thr-308, Thr-450, and Ser-473. More than one site may be orylated simultaneously. tion of the function of Akt has been ularly identified in connection with the orylation of two sites: a threonine: T308 (Akt 1), T309 (Akt 2), T305 (Akt 3), and a serine: S473 (Akt 1), S474 (Akt 2), S472 (Akt 3).

Phosphoinositide dependent kinase 1 (PDK1) is thought to phosphorylate threonine 308, while mTOR Complex 2 (mTORC2) has recently been identified as PDK2, as it is thought to phosphorylate serine 473 of Akt1.

As used herein, phospho-Akt shall refer to Akt that has been phosphorylated on one or more residues, with the proviso that for Akt1, Akt2, and Akt3 the designation phospho-Akt is used to indicate phosphorylation at a site other than T308, T309 or T305 respectively. To clarify this r, the phospho-Akt may or may not be phosphorylated at T308, T309 or T305 for Akt1, Akt2 and Akt3 respectively, but the ation phospho-Akt indicates phosphorylation at sites other than these. Phospho-Akt may optionally also be post-translationally modified in a way other than by phosphorylation.

More preferably, phospho-Akt shall refer to Akt that has been phosphorylated on the ing serine residue: for Akt1: S473; for Akt2: S474; and for Akt3: S472.

This preferred embodiment therefore does not encompass the Akt3 encoded by RAC-gamma serine/threonine-protein kinase m 2, since it does not have a serine 472.

Protein sequences coding for human Akt1, Akt2 and Akt3 are available via National Center for Biotechnology Information (NCBI) accession numbers Akt1: NP_005154.2, see SEQ. ID. No. 1 (see also NP_001014431 and 014432;), Akt2: 617.1, see SEQ ID No. 2 and Akt3: NP_005456.1: RAC-gamma serine/threonine-protein kinase isoform 1, see SEQ ID No. 3.

One ment relates to use of phospho-Akt as a biomarker for predicting the response to a compound, wherein phospho-Akt is Akt that has been phosphorylated on one or more residues, with the proviso that for Akt1, Akt2, and Akt3 the designation phospho-Akt is used to indicate phosphorylation at a site other than T308, T309 or T305 respectively, wherein the nd is a nd of general formula I, R3 R2 N R4 N R5 N N wherein R represents phenyl, thienyl or pyridinyl wherein phenyl is optionally substituted by one or two substituents independently selected from alkyl, halo-lower alkyl, hydroxy-lower alkyl, lower alkoxy-lower alkyl, acyloxy-lower alkyl, phenyl, y, lower alkoxy, hydroxy-lower alkoxy, lower alkoxy-lower alkoxy, -lower alkoxy, lower alkylcarbonyloxy, amino, monoalkylamino, dialkylamino, lower alkoxycarbonylamino, lower alkylcarbonylamino, tuted amino wherein the two substituents on nitrogen form together with the nitrogen heterocyclyl, lower alkylcarbonyl, carboxy, lower alkoxycarbonyl, cyano, n, and nitro; and wherein two adjacent substituents are methylenedioxy; and wherein pyridinyl is optionally substituted by lower alkoxy, amino or halogen; X represents a group C=Y, wherein Y stands for oxygen or nitrogen substituted by hydroxy or lower alkoxy; R1 represents hydrogen, lower alkylcarbonyl, hydroxy-lower alkyl or cyano-lower alkyl; R2, R3 and R6 represent hydrogen; R4 and R5, independently of each other, represent hydrogen, lower alkyl or lower alkoxy; or R4 and R5 together represent methylenedioxy; and pharmaceutically acceptable derivatives thereof, or wherein R represents phenyl or nyl wherein phenyl is optionally substituted by one or two substituents independently selected from alkyl, ower alkyl, y-lower alkyl, lower alkoxy-lower alkyl, acyloxy-lower alkyl, phenyl, hydroxy, lower alkoxy, y-lower alkoxy, lower -lower alkoxy, phenyl-lower alkoxy, lower alkylcarbonyloxy, amino, monoalkylamino, dialkylamino, lower alkoxycarbonylamino, lower alkylcarbonylamino, substituted amino n the two substituents on nitrogen form together with the nitrogen heterocyclyl, lower alkylcarbonyl, carboxy, lower alkoxycarbonyl, formyl, cyano, halogen, and nitro; and wherein two adjacent substituents are methylenedioxy; and wherein pyridinyl is optionally substituted by lower alkoxy, amino or halogen; X represents oxygen; R1 represents hydrogen, lower alkylcarbonyl, hydroxy-lower alkyl or cyano-lower alkyl; R2, R3 and R6 represent hydrogen; R4 and R5, independently of each other, represent hydrogen, lower alkyl or lower alkoxy; or R4 and R5 together represent methylenedioxy; and pharmaceutically acceptable derivatives f; and wherein the prefix lower s a radical having up to and including a maximum of 7, especially up to and including a maximum of 4 carbon atoms.

Preferably the response may be of a disease in a subject. Also preferably the response may be to treatment, i.e. to treatment with the compound of general formula I or pharmaceutically acceptable derivatives thereof.

The biomarker phospho-Akt is measured ex vivo in a sample or s taken from the human or animal body, preferably taken from the human body. The sample or samples are pre-obtained from the human or animal body, preferably preobtained from the human body.

In a preferred embodiment, described is use of phospho-Akt as a ker for predicting the resistance of a disease in a subject to a compound of general formula I or pharmaceutically acceptable derivatives thereof as defined above. ably the pharmaceutically acceptable derivative is ed from the group consisting of a salt, solvate, pro-drug, salt of a ug, polymorph and isomer of a compound of l formula I. Pro-drugs are ably ester and amides of naturally occurring amino acids, small peptides or pegylated hydroxy acids. More preferably, the pro-drug is an amide formed from an amino group present within the R group of the compound of general formula I and the carboxy group of glycine, alanine or lysine.

Particularly preferably the compound is N N N N O or a pharmaceutically acceptable salt thereof, preferably a hydrochloride salt thereof, most preferably a dihydrochloride salt thereof.

Also described is a method for predicting the response of a disease in a subject to a compound of general formula I or pharmaceutically acceptable derivatives f as defined above, comprising the steps of: a) ing a level of phospho-Akt as defined above in a sample preobtained from the subject to obtain a value or values representing this level; and b) ing the value or values from step a) to a standard value or set of standard values.

Further preferably the response which is predicted is resistance.

The measuring of a level or levels of phospho-Akt is performed ex-vivo in a sample pre-obtained from the subject. Pre-obtained refers to the fact that the sample is obtained before it is subjected to any method involving ing the level of the biomarker, and pre-obtained is not to be understood as in relation to treatment.

In a preferred ment, a higher level of phospho-Akt in the sample from the subject relative to the standard value or set of standard values predicts resistance.

Also preferably, the disease is a stic or autoimmune disease. More preferably the disease is cancer. Especially preferably the cancer is ed from the group consisting of breast cancer, prostate cancer, cervical cancer, gastric cancer, ovarian , colorectal cancer (i.e including colon cancer and rectal cancer), pancreatic cancer, liver cancer, brain cancer, neuroendocrine cancer, lung , kidney cancer, hematological malignancies, melanoma and sarcomas. More especially preferably the cancer is selected from the group consisting of breast , cervical cancer, gastric cancer, lung cancer, ctal cancer and melanoma. Particularly preferably the cancer is selected from the group consisting of gastric , lung cancer, colorectal cancer and melanoma.

Also described is a method of treating a neoplastic or autoimmune disease, preferably , in a subject in need thereof, comprising measuring a level of phospho-Akt as d above in a sample from the subject to obtain a value or values representing this level, and treating the subject with a compound of general formula I or a pharmaceutically acceptable derivative thereof as defined above, if the level of phospho-Akt in said sample is not higher than a standard value or set of standard values.

Also described is phospho-Akt as defined above for use in the treatment of a neoplastic or autoimmune disease, preferably cancer, comprising measuring a level of phospho-Akt in a sample from a subject to obtain a value or values representing this level, and treating the subject with a compound of general formula I or a pharmaceutically acceptable derivative thereof as defined above, if the level of phospho-Akt is not higher than a standard value or set of standard values.

The measuring of a level of phospho-Akt is med ex-vivo in a sample pre-obtained from the subject.

Also described is a method of treating a neoplastic or autoimmune e, preferably cancer, by first decreasing a level of phospho-Akt as d above in a subject that has a sample with a higher level of phospho-Akt compared to a rd level or set of standard levels, then treating the subject with a compound of general formula I or a pharmaceutically acceptable derivative thereof as defined above.

Also described is a kit for predicting the response to a compound of general formula I or a pharmaceutically acceptable derivative thereof, as defined above, comprising reagents necessary for measuring the level of phospho-Akt as defined above in a sample. More ably the kit also comprises a comparator module which comprises a standard value or set of standard values to which the level of phospho-Akt in the sample is compared.

More preferably the kit comprises a compound of general formula I or a pharmaceutically acceptable derivative thereof as defined above. In an especially red ment the kit comprises a compound of the following formula or a pharmaceutically able salt thereof N N N N O Chemical name: S-2,6-Diamino-hexanoic acid [4-(2-{2-[4-(2-cyanoethylamino zanyl]-benzoimidazolyl}-acetyl)-phenyl]-amide In a particularly preferred embodiment the pharmaceutically acceptable salt is a dihydrochloride salt.

Also bed is a device for predicting the response to a compound of general formula I or a pharmaceutically acceptable derivative thereof as defined above, comprising reagents necessary for measuring a level of phospho-Akt as defined above in a sample and a ator module which comprises a standard value or set of standard values to which the level of o-Akt in the sample is compared.

In a preferred embodiment, the reagents in the kit or device comprise a capture t comprising a detector for phospho-Akt, and a detector reagent.

Especially preferably the capture reagent is an antibody. Also preferably, the disease is predicted to be resistant to treatment with said compound when phospho-Akt is higher relative to a standard value or set of standard values. In a preferred embodiment, the comparator module is included in instructions for use of the kit. In another preferred embodiment the comparator module is in the form of a display device.

Embodiments of the present invention will now be described by way of example with reference to the accompanying figures. The invention however is not to be understood as limited to these embodiments.

Brief Description of the Figures Figure 1: Shows the treatment of human tumour cell lines from different histotypes with 50 nM BAL27862. The microtubules of mitotic or G2/M arrested cells were d after 24 hours ent with 50 nM BAL27862 or vehicle l.

Fig. 1A and 1B: A549 NSCLC cells; Fig. 1C and 1D: HeLa cervical cancer cells; Fig. 1E and 1F: SKBR3 breast cancer cells e l treatment: Figures 1A, 1C & 1E, BAL27862 treatment: Figures 1B, 1D & 1F.

Figure 2: Shows the treatment of A549 NSCLC cells with the Compounds B and C. The microtubules of mitotic or G2/M arrested A549 NSCLC cells were stained after 24 hours ent with 80 nM or 20 nM of Compounds B and C, respectively.

The white scale bar ents 10 micrometres.

Fig. 2A: treatment with 20 nM compound C Fig. 2B: treatment with 80 nM compound B Figure 3: Shows a comparison of treatment of cells with BAL27862 compared to conventional ubule targeting agents. Microtubules of mitotic or G2/M ed A549 NSCLC cells were stained after 24 hours of treatment with 50 nM of A: BAL27862; B: vinblastine; C: colchicine; D: paclitaxel. Stacks of images taken every 1 µm were processed by using ImageJ software.

Figure 4: Shows a comparison of treatment of A549 NSCLC cells with BAL27862 compared to nocodazole. Microtubules of mitotic or G2/M arrested cells were stained after 24 h of treatment with s concentrations of nocodazole (B, C & D) and BAL27862 (E, F & G). A: control, B: Nocodazole 50 nM, C: Nocodazole 100 nM, D: zole 200 nM, E: BAL27862 20 nM; F: BAL27862 30 nM and G: BAL27862 50 nM. The white scale bar represents 10 micrometres. Representative images of the microtubule phenotypes observed are shown.

Figure 5: Shows a combination of treatment with BAL27862 and conventional microtubule-targeting agents. Microtubules of c or G2/M arrested A549 NSCLC cells were d after treatment for the times indicated below. 50 nM BAL27862, 50 nM vinblastine, 50 nM colchicine and 25 nM paclitaxel were used. The white scale bar represents 10 micrometres.

Fig. 5A: 24 hours vinblastine treatment; Fig. 5B: 24 hours vinblastine treatment with the final 4 hours including BAL27862; Fig. 5C: 24 hours BAL27862 treatment with the final 4 hours ing vinblastine.

Fig. 5D: 24 hours colchicine treatment; Fig. 5E: 24 hours colchicine treatment with the final 4 hours including BAL27862; Fig. 5F: 24 hours BAL27862 treatment with the final 4 hours including colchicine.

Fig. 5G: 24 hours paclitaxel treatment; Fig. 5H: 24 hours paclitaxel treatment with the final 4 hours ing Fig. 5I: 24 hours BAL27862 ent with the final 4 hours including paclitaxel.

Figure 6: Shows a ation of treatment with BAL27862 and nocodazole.

Microtubules of mitotic or G2/M arrested A549 NSCLC cells were stained after ent for the times indicated below. 25 nM BAL27862 and nocodazole at the concentrations indicated below were used. The white scale bar represents 10 micrometers.

Fig. 6A: 24 hours control treatment; Fig. 6B: 24 hours of 25 nM BAL27862 treatment; Fig. 6C: 24 hours of 50 nM nocodazole treatment Fig. 6D: 24 hours of 100 nM nocodazole treatment Fig. 6E: 24 hours of 150 nM nocodazole treatment Fig. 6F: 24 hours of 200 nM zole treatment Fig. 6G: 24 hours of 50 nM zole treatment with the final 4 hours including 25 nM BAL27862; Fig. 6H: 24 hours of 100 nM nocodazole treatment with the final 4 hours including 25 nM BAL27862; Fig. 6I: 24 hours of 150 nM nocodazole treatment with the final 4 hours including 25 nM BAL27862; Fig. 6J: 24 hours of 200 nM nocodazole treatment with the final 4 hours including 25 nM BAL27862; Fig. 6K: 24 hours of 25 nM BAL27862 treatment with the final 4 hours including 50 nM nocodazole; Fig. 6L: 24 hours of 25 nM BAL27862 treatment with the final 4 hours including 100 nM nocodazole; Fig. 6M: 24 hours of 25 nM BAL27862 treatment with the final 4 hours including 150 nM nocodazole; Fig. 6N: 24 hours of 25 nM BAL27862 treatment with the final 4 hours including 200 nM nocodazole.

Figure 7: Shows protein extracts prepared from patient-derived gastric cancer (Fig. 7A), lung cancer (Fig. 7B), colorectal cancer (Fig. 7C) and melanoma (Fig. 7D) tumours obtained from subcutaneously afted nude mice, and analysed by immunoblotting for phospho-Akt and Akt expression, with actin ed as a loading l. Three independent tumours were analysed in each case (1 – 3).

BAL27862, paclitaxel and vinblastine resistance and sensitivity of the tumour cells using an ex vivo colony outgrowth assay is as defined in Table 1.

Figure 8: Shows that tumour cell phospho-Akt levels are increased in a t-derived afted gastric tumour model defined as BAL27862 resistant by ex vivo colony outgrowth is. Patient-derived tumour xenografts ained in nude mice) were prepared, fixed and stained for phospho-Akt protein expression using immunohistochemistry. BAL27862, paclitaxel and vinblastine resistance and sensitivity of the tumour cells using an ex vivo colony outgrowth assay is as defined in Table 1.

Figure 9: shows the protein sequence of Akt1 (RAC-alpha /threonine- protein kinase) [Homo sapiens] (SEQ. ID. No. 1) Figure 10: shows the protein sequence of Akt2 (RAC-beta serine/threonineprotein kinase) [Homo sapiens] (SEQ. ID. No. 2) Figure 11: shows the protein acid sequence of Akt3 (RAC-gamma serine/threonine-protein kinase isoform 1) [Homo sapiens] (SEQ. ID. No. 3) Detailed Description Compounds of a I The compounds described herein are represented by general formula I: R3 R2 N R4 N R5 N N wherein R represents phenyl, thienyl or pyridinyl n phenyl is optionally substituted by one or two substituents independently selected from alkyl, halo-lower alkyl, hydroxy-lower alkyl, lower -lower alkyl, acyloxy-lower alkyl, phenyl, hydroxy, lower alkoxy, hydroxy-lower alkoxy, lower -lower alkoxy, phenyl-lower alkoxy, lower alkylcarbonyloxy, amino, monoalkylamino, lamino, lower alkoxycarbonylamino, lower alkylcarbonylamino, substituted amino wherein the two substituents on nitrogen form together with the nitrogen heterocyclyl, lower alkylcarbonyl, carboxy, lower alkoxycarbonyl, cyano, n, and nitro; and wherein two adjacent substituents are methylenedioxy; and wherein pyridinyl is optionally substituted by lower alkoxy, amino or halogen; X represents a group C=Y, wherein Y stands for oxygen or nitrogen substituted by hydroxy or lower alkoxy; R1 ents hydrogen, lower alkylcarbonyl, hydroxy-lower alkyl or cyano-lower alkyl; R2, R3 and R6 represent hydrogen; R4 and R5, independently of each other, represent hydrogen, lower alkyl or lower alkoxy; or R4 and R5 together represent methylenedioxy; and pharmaceutically acceptable derivatives thereof, or wherein R represents phenyl or pyridinyl wherein phenyl is ally substituted by one or two substituents independently selected from alkyl, halo-lower alkyl, hydroxy-lower alkyl, lower alkoxy-lower alkyl, acyloxy-lower alkyl, phenyl, hydroxy, lower alkoxy, hydroxy-lower alkoxy, lower -lower alkoxy, phenyl-lower alkoxy, lower alkylcarbonyloxy, amino, monoalkylamino, dialkylamino, lower alkoxycarbonylamino, lower alkylcarbonylamino, substituted amino wherein the two substituents on nitrogen form together with the nitrogen heterocyclyl, lower arbonyl, carboxy, lower alkoxycarbonyl, formyl, cyano, halogen, and nitro; and wherein two adjacent tuents are enedioxy; and n pyridinyl is optionally substituted by lower alkoxy, amino or n; X represents oxygen; R1 represents hydrogen, lower alkylcarbonyl, hydroxy-lower alkyl or cyano-lower alkyl; R2, R3 and R6 represent hydrogen; R4 and R5, independently of each other, represent en, lower alkyl or lower alkoxy; or R4 and R5 together represent methylenedioxy; and pharmaceutically acceptable derivatives thereof; and wherein the prefix lower denotes a l having up to and including a maximum of 7, especially up to and including a maximum of 4 carbon atoms.

Heterocyclyl designates preferably a saturated, partially ted or unsaturated, mono- or bicyclic ring containing 4-10 atoms comprising one, two or three heteroatoms selected from nitrogen, oxygen and sulfur, which may, unless otherwise specified, be carbon or nitrogen linked, wherein a ring nitrogen atom may optionally be substituted by a group selected from lower alkyl, amino-lower alkyl, aryl, aryl-lower alkyl and acyl, and a ring carbon atom may be substituted by lower alkyl, amino-lower alkyl, aryl, aryl-lower alkyl, heteroaryl, lower alkoxy, hydroxy or oxo.

Examples of heterocyclyl are pyrrolidinyl, oxazolidinyl, thiazolidinyl, dinyl, morpholinyl, piperazinyl, dioxolanyl and tetrahydropyranyl.

Acyl ates, for example, alkylcarbonyl, cyclohexylcarbonyl, arylcarbonyl, aryl-lower alkylcarbonyl, or heteroarylcarbonyl. Lower acyl is preferably lower arbonyl, in particular propionyl or acetyl.

Preferably, the compound of general formula I is defined as wherein R1 is selected from the group consisting of hydrogen, acetyl, CH2CH 2CN and CH 2CH 2CH 2OH.

In one preferred embodiment, the compound of general formula I is selected from the group consisting of: 4-(1-Phenacyl-1H-benzimidazolyl)-furazanylamine, 4-Bromophenacyl)-1H-benzimidazolyl]-furazanylamine oxime, N-{4-[1-(4-Chlorophenacyl)-1H-benzimidazolyl]-furazanyl}-acetamide, 4-[1-(4-Chlorophenacyl)-1H-benzimidazolyl]-furazanyl-N-(2-cyanoethyl)-amine, 4-[1-(4-Chlorophenacyl)-1H-benzimidazolyl]-furazanyl-N-(3-hydroxypropyl)- amine, 4-[1-(3-Aminochlorophenacyl)-1H-benzimidazolyl]-furazanylamine, 4-[1-(3-Methoxymethoxymethoxy-phenacyl)-1H-benzimidazolyl]-furazan ylamine, and pharmaceutically able derivatives thereof.

In r preferred embodiment, the compound of general formula I is: N N R, Y and R1 are defined as follows: R Y R1 O H NOH H NOMe H O H NOH H NOH H NOMe H MeO O H MeO NOH H MeO NOMe H O H NOH H NOMe H O H NOMe H O H Cl Cl O H NOH H NOMe H Cl O H Cl NOH H Cl NOMe H NOMe H O H Et2N O Ac O H O H O O H O CN O CH2CH2CN O H O H Me O CH2CH2CH2OH Me O H Me O CH2CH2CN O H O CN O CH2CH2CN O CH2CH2CN N O H O H A cN H O H O2N O H A cH N O2N O H O2N O H O H O2N O H 2 O CN O H F O H O H MeO O H MeO O H MeO O H MeO O H O H O H NH N O CH2CH2CN NH N OH O H O H O O O O O CH2CH2CN MeO N or pharmaceutically acceptable derivatives f.

In yet another preferred embodiment, the compound of general formula I is selected from the group consisting of: 4-(1-Phenoxymethyl-1H-benzimidazolyl)-furazanylamine, 4-[1-(4-Fluorophenoxymethyl)-1H-benzimidazolyl]-furazanylamine, 3,4-Dimethylphenoxymethyl)-1H-benzimidazolyl]-furazanyl-N-(2- cyanoethyl)-amine, and compounds represented by the formula: N N N N O wherein R and R1 are as defined below R R1 CH2CH2CN CH2CH2CN CH2CH2CN CH2CH2CH2OH Cl N NH N or pharmaceutically acceptable derivatives thereof.

In still yet r preferred embodiment the compound of general formula I is: R4 N N R5 N N wherein R, R4 and R5 are as defined below R R4 R5 Me Me Me Me Me Me Me Me Me Me OMe OMe OMe OMe OMe OMe OMe OMe OMe OMe or ceutically acceptable derivatives thereof.

More preferably, the compound is a compound of general formula I R3 R2 N R4 N R5 N N wherein R represents phenyl or pyridinyl wherein phenyl is optionally substituted by one or two substituents independently selected from lower alkyl, lower alkoxy, amino, acetylamino, n and nitro; and wherein pyridinyl is optionally substituted by amino or halogen; X represents a group C=O; R1 represents hydrogen or cyano-lower alkyl; R2, R3, R4, R5 and R6 represent hydrogen; and pharmaceutically able derivatives thereof, and wherein the prefix lower denotes a l having up to and including a maximum of 7, especially up to and including a maximum of 4 carbon atoms.

Especially preferably, the compound is represented by the following formula N N wherein R, Y and R1 are defined as s: R Y R1 O H O CH2CH2CN O H NH N O CH2CH2CN NH N or pharmaceutically acceptable derivatives thereof.

More especially preferably, the compound is represented by the following formula N N wherein R, Y and R1 are defined as follows: R Y R1 O CH2CH2CN O H O CH2CH2CN NH N or pharmaceutically acceptable tives thereof.

Particularly preferably, the compound of general formula I is N N N N O or pharmaceutically acceptable derivatives thereof.

The term derivative or derivatives in the phrase “pharmaceutically acceptable derivative” or “pharmaceutically acceptable derivatives” of compounds of general a I relates to salts, solvates and xes thereof and to es and complexes of salts thereof, as well as to pro-drugs, polymorphs, and isomers thereof (including optical, geometric and tautomeric isomers) and also salts of prodrugs thereof. In a more preferred embodiment, it relates to salts and pro-drugs, as well as to salts of pro-drugs thereof.

Salts are ably acid addition salts. Salts are formed, preferably with organic or inorganic acids, from compounds of a (I) with a basic nitrogen atom, especially the pharmaceutically acceptable salts. Suitable inorganic acids are, for example, halogen acids, such as hydrochloric acid, sulfuric acid, or phosphoric acid. le organic acids are, for example, carboxylic, phosphonic, sulfonic or sulfamic acids, for example acetic acid, propionic acid, octanoic acid, decanoic acid, dodecanoic acid, glycolic acid, lactic acid, fumaric acid, succinic acid, adipic acid, c acid, suberic acid, azelaic acid, malic acid, tartaric acid, citric acid, amino acids, such as glutamic acid or aspartic acid, maleic acid, hydroxymaleic acid, methylmaleic acid, cyclohexanecarboxylic acid, adamantanecarboxylic acid, benzoic acid, lic acid, 4-aminosalicylic acid, phthalic acid, phenylacetic acid, mandelic acid, cinnamic acid, methane- or ethane-sulfonic acid, 2-hydroxyethanesulfonic acid, ethane-1,2-disulfonic acid, benzenesulfonic acid, 2-naphthalenesulfonic acid, 1,5- alene-disulfonic acid, 2-, 3- or 4-methylbenzenesulfonic acid, methylsulfuric acid, ethylsulfuric acid, dodecylsulfuric acid, ohexylsulfamic acid, N-methyl-, N- ethyl- or N-propyl-sulfamic acid, or other organic protonic acids, such as ascorbic acid.

The compound may be administered in the form of a pro-drug which is broken down in the human or animal body to give a compound of the formula I.

Examples of pro-drugs include in vivo hydrolysable esters and amides of a compound of the formula I. ular pro-drugs considered are ester and amides of naturally occurring amino acids and ester or amides of small peptides, in particular small peptides consisting of up to five, preferably two or three amino acids, as well as esters and amides of pegylated hydroxy acids, preferably hydroxy acetic acid and lactic acid. Pro-drug esters are formed from the acid on of the amino acid or the C terminal of the peptide and suitable hydroxy group(s) in the compound of formula I. ug amides are formed from the amino function of the amino acid or the N terminal of the peptide and suitable carboxy group(s) in the compound of formula I, or from the acid function of the amino acid or the C terminal of the peptide and le amino group(s) in the compound of formula I. Particularly preferably the pro-drug amides are formed from the amino group(s) present within the R group of formula I.

More preferably, the pro-drug is formed by the on of glycine, alanine or lysine to the compound of formula I.

Even more preferably the nd of general formula I is in the form of a pro-drug selected from the compounds of formulae: N N NH N N N N O N N N N N O N N O O O O O H N O N NH H 2 NH2 NH2 H2N , , , N N NH N N N N O N N N N N O N N O O O O N N O H N O N NH H 2 NH2 NH2 H2N , , , H N N N 2 H N N 2 N N O N N N N N O N N O O O O O H N O H N NH NH2 NH , 2 and H N 2 .

In an ally preferred embodiment the compound is in the form of a prodrug which has the following formula N N N N O In a most especially preferred embodiment the nd is a pharmaceutically acceptable salt, preferably a hydrochloride salt thereof, most preferably a dihydrochloride salt thereof, of a compound of the following formula N N N N O The pharmaceutically active metabolite in vivo in this case is BAL27862.

These pro-drugs may be prepared by processes that are known per se, in particular, a process, wherein a compound of formula (II) N N N N O (II) wherein R1 is defined as for formula (I) and Z is CH or N, or a derivative of such a compound comprising functional groups in ted form, or a salt thereof is (1) acylated with an amino acid of formula (III) R11 (III) R10 is selected from hydrogen (Gly); methyl (Ala) and protected aminobutyl (Lys) and R11 is a suitable amino protecting group, and (2) any protecting groups in a protected derivative of the resulting compound are removed to yield a pro-drug as shown above, and, if so desired, (3) said pro-drug is converted into a salt by treatment with an acid, or a salt of a compound of formula (II) is ted into the corresponding free nd of a (II) or into another salt, and/or a mixture of isomeric product compounds is separated into the individual isomers.

Acylation of a compound of formula (II) with an amino acid of formula (III) is performed in a manner known per se, usually in the presence of a suitable polar or dipolar aprotic solvent, with cooling or heating as ed, for example in a ature range from approximately minus 80°C to approximately plus 150°C, more preferably from minus 30°C to plus 120°C, especially in a range from imately around 0°C to the reflux ature of the used solvent. Optionally a suitable base is added, in particularly an aromatic base like pyridine or collidine or a tertiary amine base such as triethylamine or diisopropylethylamine, or an inorganic basic salt, e.g. potassium or sodium carbonate.

Acylation may be accomplished under conditions used for amide formation known per se in peptide chemistry, e.g. with activating agents for the carboxy group, such as iimides like N,N’-diethyl-, N,N’-dipropyl-, N,N’-diisopropyl-, N,N’- dicyclohexylcarbodiimide and N-(3-dimethylaminoisopropyl)-N’-ethylcarbodiimide- hydrochloride (EDC), or with agents such as 1-hydroxybenzotriazole (HOBt), benzotriazolyloxytris(dimethylamino)-phosphonium hexafluorophosphate (BOP), O-(7-aza-benzotriazolyl)-N,N,N’,N’-tetramethyl-uronium hexafluorophosphate (HATU), 2-(2-oxo(2H)-pyridyl)-1,1,3,3-tetramethyluronium tetrafluoroborate (TPTU), optionally in the presence of suitable bases, catalysts or co-reagents. The carboxy group may also be activated as acyl halogenide, preferably as acyl chloride, e.g. by reaction with thionylchloride or oxalylchloride, or as symmetrical or unsymmetrical anhydride, e.g. by reaction with halogeno formates like ethyl chloroformate, optionally in the presence of suitable bases, sts or co-reagents.

If one or more other functional groups, for e carboxy, hydroxy or amino, are or need to be protected in a compound of formula (II) or (III), because they should not take part in the reaction, these are such protecting groups as are usually applied in the synthesis of amides like, in particular peptide compounds, cephalosporins, penicillins, nucleic acid derivatives and sugars, which are known to the skilled persons. Suitable protecting groups for amino groups are for example tbutyl carbamate, benzyl carbamate or renylmethyl carbamate.

The ting groups may already be present in precursors and should t the functional groups concerned against unwanted secondary reactions, such as alkylations, acylations, etherifications, esterifications, ions, solvolysis, and similar reactions. It is a characteristic of protecting groups that they lend themselves readily, i.e. without undesired secondary reactions, to removal, typically by solvolysis, reduction, photolysis or also by enzyme activity, for e under ions analogous to physiological conditions, and that they are not present in the end products. The specialist knows, or can easily establish, which protecting groups are suitable with the reactions mentioned hereinabove and hereinafter.

The protection of such functional groups by such protecting groups, the protecting groups themselves, and their removal reactions are bed for example in standard reference books for peptide sis and in special books on protective groups such as J. F. W. McOmie, "Protective Groups in Organic Chemistry", Plenum Press, London and New York 1973, in den der organischen Chemie" (Methods of c chemistry), Houben-Weyl, 4th n, Volume 15/I, Georg Thieme Verlag, Stuttgart 1974, and in T. W. Greene, G. M. Wuts "Protective Groups in Organic Synthesis", Wiley, New York, 2006.

Disease The compounds of l formula I have been shown to arrest cell proliferation and induce cell death, for example by apoptosis.

Deregulation of cell proliferation, or lack of appropriate cell death, has wide ranging clinical implications. A number of diseases associated with such deregulation involve hyperproliferation, inflammation, tissue remodeling and repair. Familiar indications in this category include cancers, restenosis, neointimal hyperplasia, angiogenesis, triosis, lymphoproliferative disorders, transplantation related pathologies (graft rejection), polyposis, loss of neural on in the case of tissue remodeling and the like.

Cancer is associated with abnormal cell eration and cell death rates. As apoptosis is inhibited or delayed in most types of proliferative, neoplastic diseases, induction of apoptosis is an option for treatment of cancer, especially in cancer types which show resistance to classic chemotherapy, radiation and immunotherapy (Apoptosis and Cancer Chemotherapy, Hickman and Dive, eds., Blackwell Publishing, 1999). Also in mune and transplantation related diseases and pathologies compounds inducing apoptosis may be used to restore normal cell death processes and therefore can eradicate the symptoms and might cure the diseases.

Further applications of compounds inducing apoptosis may be in restenosis, i. e. accumulation of vascular smooth muscle cells in the walls of arteries, and in persistent infections caused by a failure to ate bacteria-and virus-infected cells.

Furthermore, apoptosis can be induced or reestablished in epithelial cells, in endothelial cells, in muscle cells, and in others which have lost contact with extracellular matrix.

A compound ing to general formula I or pharmaceutically acceptable derivatives thereof may be used for the prophylactic or especially therapeutic treatment of the human or animal body, in particular for treating a neoplastic disease, autoimmune disease, lantation related pathology and/or rative disease.

Examples of such neoplastic diseases e, but are not limited to, epithelial neoplasms, squamous cell neoplasms, basal cell neoplasms, transitional cell papillomas and carcinomas, adenomas und adenocarcinomas, adnexal and skin appendage neoplasms, mucoepidermoid neoplasms, cystic neoplasms, mucinous and serous neoplasms, ducal-, lobular and ary neoplasms, acinar cell sms, complex epithelial neoplasms, specialized gonadal neoplasms, ngliomas and glomus tumours, naevi and melanomas, soft tissue tumours and sarcomas, fibromatous neoplasms, myxomatous neoplasms, lipomatous neoplasms, myomatous neoplasms, complex mixed and stromal neoplasms, fibroepithelial neoplasms, synovial like neoplasms, mesothelial neoplasms, germ cell neoplasms, trophoblastic neoplasms, mesonephromas, blood vessel tumours, lymphatic vessel tumours, osseous and omatous neoplasms, giant cell tumours, laneous bone tumours, odontogenic tumours, s, neuroepitheliomatous neoplasms, meningiomas, nerve sheath tumours, granular cell tumours and alveolar soft part sarcomas, Hodgkin's and non-Hodgkin’s lymphomas, other lymphoreticular neoplasms, plasma cell tumours, mast cell tumours, immunoproliferative es, leukemias, miscellaneous myeloproliferative disorders, lymphoproliferative disorders and ysplastic syndromes.

The compounds of general formula I or pharmaceutically acceptable derivatives thereof may be used to treat autoimmune diseases. Examples of such mune diseases e, but are not limited to, systemic, d or subacute cutaneous lupus erythematosus, rheumatoid arthritis, antiphospholipid syndrome, CREST, ssive ic sclerosis, mixed connective tissue disease (Sharp syndrome), Reiter's syndrome, juvenile arthritis, cold inin disease, essential mixed cryoglobulinemia, tic fever, ankylosing spondylitis, chronic polyarthritis, myasthenia gravis, multiple sclerosis, chronic inflammatory demyelinating polyneuropathy, Guillan-Barre syndrome, dermatomyositis/ ositis, autoimmune hemolytic , thrompocytopenic purpura, neutropenia, type I diabetes mellitus, thyroiditis (including Hashimoto's and Grave'disease), Addison's disease, polyglandular syndrome, pemphigus (vulgaris, foliaceus, sebaceous and vegetans), bullous and cicatricial pemphigoid, goid gestationis, epidermolysis bullosa acquisita, linear IgA disease, lichen sus et atrophicus, morbus Duhring, psoriasis vulgaris, e, generalized pustular and localized pustular psoriasis, vitiligo, alopecia areata, primary biliary cirrhosis, autoimmune hepatitis, all forms of glomerulonephritis, pulmonal hemorrhage (goodpasture syndrome), IgA nephropathy, pernicious anemia and mune gastritis, inflammatory bowel diseases (including colitis ulcerosa and morbus Crohn), Behcet's e, Celic- Sprue disease, autoimmune uveitis, autoimmune myocarditis, granulomatous orchitis, aspermatogenesis without orchitis, idiopatic and secondary pulmonary fibrosis, matory diseases with a possibility of autoimmune pathogenesis, such as pyoderma gangrensosum, lichen ruber, sarcoidosis (including Lofgren and cutaneous/subcutaneous type), oma anulare, allergic type I and type IV immunolgical reaction, asthma bronchiale, pollinosis, atopic, contact and airborne dermatitis, large vessel vasculitis (giant cell and Takayasu's tis), medium sized vessel vasculitis (polyarteritis nodosa, Kawasaki disease), small vessel vasculitis (Wegener's granulomatosis, Churg Strauss syndrome, copic polangiitis, HenochSchoenlein a, essential cryoglobulinemic vasculitis, ous lastic angiitis), hypersensitivity syndromes, toxic epidermal necrolysis (Stevens-Johnson syndrome, erythema multiforme), diseases due to drug side effects, all forms of cutaneous, organ- specific and systemic effects due to type l-vu (Coombs classification) immunologic forms of reaction, transplantation related pathologies, such as acute and chronic graft versus host and host versus graft disease, involving all organs (skin, heart, kidney, bone marrow, eye, liver, spleen, lung, muscle, central and peripheral nerve , connective tissue, bone, blood and tic vessel, genito-urinary system, ear, cartillage, primary and secondary lymphatic system including bone marrow, lymph node, thymus, gastrointestinal tract, including oro-pharynx, geus, stomach, small intestine, colon, and rectum, including parts of above ned organs down to single cell level and substructures, e. g. stem cells).

Particularly preferably, the disease is a neoplastic or autoimmune disease.

In an especially preferred embodiment the disease is cancer.

Examples of cancers in terms of the organs and parts of the body affected include, but are not limited to, the breast, cervix, ovaries, colon, rectum, (including colon and rectum i.e. colorectal cancer), lung, (including small cell lung cancer, nonsmall cell lung cancer, large cell lung cancer and elioma), endocrine system, bone, adrenal gland, thymus, liver, stomach, intestine, (including gastric cancer), as, bone marrow, hematological malignancies, (such as ma, leukemia, myeloma or lymphoid ancies), r, urinary tract, kidneys, skin, thyroid, brain, head, neck, prostate and testis. Preferably the cancer is selected from the group ting of breast cancer, prostate cancer, cervical cancer, ovarian cancer, gastric cancer, colorectal cancer, pancreatic , liver , brain cancer, neuroendocrine cancer, lung , kidney cancer, logical malignancies, melanoma and sarcomas. Especially preferably the cancer is selected from the group consisting of breast cancer, cervical cancer, gastric cancer, lung cancer, colorectal cancer and melanoma. More especially preferably the cancer is selected from the group consisting of gastric cancer, lung cancer, colorectal cancer and melanoma.

Samples The ement of the level of phospho-Akt may be performed in vitro, on a sample of biological material derived from the subject. The sample may be any biological material separated from the body such as, for e, normal tissue, tumour tissue, cell lines, plasma, serum, whole blood, cerebrospinal fluid, lymph fluid, circulating tumour cells, cell lysate, tissue lysate, urine and aspirates. Preferably the sample is derived from the group consisting of normal tissue, tumour tissue, cell lines and circulating tumour cells. More preferably the sample is derived from tumour tissue or circulating tumour cells. In one particularly preferred embodiment the sample is derived from tumour tissue. For example, the level of phospho-Akt may be measured in a fresh, frozen or formalin fixed/paraffin embedded tumour tissue sample.

The sample is pre-obtained from the subject before the sample is subjected to the method steps involving measuring the level of the biomarker. The methods for removal of the sample are well known in the art, and it may for example be removed from the subject by biopsy, for example by punch biopsy, core biopsy, aspiration fine needle biopsy, endoscopic biopsy, or surface biopsy. A whole blood, plasma or serum sample may be collected by venipuncture and further processed according to rd techniques. Circulating tumour cells may also be obtained from blood based on, for example, size (e.g. ISET - Isolation by Size of lial Tumour cells) or immunomagnetic cell enrichment (e.g. CellSearch®, Veridex, Raritan, NJ).

Sample comparison The t may be human or animal. Preferably the t is human.

The biomarker phospho-Akt is measured ex vivo in a sample or s taken from the human or animal body, preferably taken from the human body. The sample or samples are pre-obtained from the human or animal body, preferably preobtained from the human body before the sample is subjected to the method steps involving measuring the level of the biomarker.

A ker is in general a substance that is used as an indicator of a biological response, preferably as an tor of the susceptibility to a given treatment, which in the present application is treatment with a compound of general formula I or pharmaceutically acceptable derivatives thereof.

In a particularly preferred embodiment, higher phospho-Akt levels in the sample relative to a standard value or set of standard values ts resistance.

The higher phospho-Akt levels may arise due to higher total Akt levels in the sample and/or a higher percentage of Akt which becomes phosphorylated.

As used herein, an increase or relatively high or high or higher levels relative to a standard level or set of standard levels means the amount or concentration of the biomarker in a sample is detectably greater in the sample relative to the standard level or set of rd . This encompasses at least an increase of, or higher level of, about 1% relative to the standard, preferably at least an increase of about % relative to the standard. More preferably it is an increase of, or higher level of, at least about 10% relative to the rd. More particularly preferably it is an increase of, or higher level of, at least about 20% relative to the standard. For e, such an increase of, or higher level of, may include, but is not limited to, at least about 1%, about 10%, about 20%, about 30%, about 50%, about 70%, about 80%, about 100%, about 150% or about 200% or more relative to the standard.

Preferably, higher phospho-Akt levels in a sample or samples i) relative to a standard value or set of standard values from subjects with the same tumour histotype; or ii) relative to a standard value or set of rd values from normal cells, tissue or body fluid; are predictive of ance.

The measuring of a level of phospho-Akt is performed ex-vivo in a sample pre-obtained from the subject.

Especially preferably, higher phospho-Akt levels in a sample or samples relative to a standard value or set of rd values from subjects with the same tumour histotype are predictive of resistance.

In one preferred embodiment, for the case i) where the measurement is compared in a sample or samples relative to a standard value or set of standard values from samples from subjects with the same tumour histotype as the sample to which it is to be compared, the standard value or set of standard values are established from samples from a tion of subjects with that cancer type. The samples from these standard subjects may for e be d from the tumour tissue or circulating tumour cells, as long as the origin of the sample is consistent between the standard and the sample to be compared.

In another preferred embodiment, for the case ii) where the measurement is compared in a sample or samples relative to a standard value or set of standard values taken from normal cells or tissue, the standard value or set of standard values may be established from a sample of normal (e.g. non-tumorous) cells, tissue or body fluid. Such data may be gathered from a population of subjects in order to develop the rd value or set of standard values.

The standard value or set of standard values are established ex-vivo from pre-obtained samples which may be from cell lines, or preferably biological material taken from at least one subject and more preferably from an average of subjects (e.g., n=2 to 1000 or more). The standard value or set of standard values may then be correlated with the response data of the same cell lines, or same subjects, to treatment with a compound of general formula I or a pharmaceutically able derivative f. From this correlation a comparator module, for example in the form of a relative scale or scoring system, optionally including cut-off or threshold values, can be established which tes the levels of biomarker associated with a spectrum of response levels to the compound of formula I or a pharmaceutically able derivative thereof. The um of response levels may se relative sensitivity to the therapeutic activity of the compound, (e.g. high sensitivity to low sensitivity), as well as resistance to the therapeutic activity. In a preferred embodiment this comparator module comprises a f value or set of values which predicts resistance to treatment.

For example, if an immunohistochemical method is used to measure the level of phospho-Akt in a sample, standard values may be in the form of a scoring system. Such a system might take into account the percentage of cells in which staining for phospho-Akt is present. The system may also take into account the relative intensity of staining or cellular localisation in the individual cells. The standard values or set of standard values of the level of phospho-Akt may then be ated with data indicating the response, ally resistance, of the subject or tissue or cell line to the therapeutic activity of a compound of a I or a ceutically acceptable tive thereof. Such data may then form part of a comparator module. se is the reaction of the cell lines, or preferably of the subject, or more preferably of the disease in a subject, to the therapeutic activity of a compound of general formula I or a pharmaceutically acceptable derivative thereof. The spectrum of response levels may comprise relative sensitivity to the therapeutic activity of the compound, (e.g. high sensitivity to low ivity), as well as resistance to the therapeutic ty. The response data may for example be monitored in terms of: objective response rates, time to disease progression, progression free survival, and overall survival.

The response of a cancerous e may be evaluated by using criteria well known to a person in the field of cancer treatment, for example but not restricted Response Evaluation Criteria in Solid Tumors (RECIST) Guidelines, Source: Eisenhauer EA, Therasse P, Bogaerts J, Schwartz LH, Sargent D, Ford R, Dancey J, Arbuck S Gwyther S, Mooney M, Rubinstein L, Shankar L, Dodd L, Kaplan R, Lacombe D, Verweij J. New response evaluation criteria in solid tumours: revised RECIST guideline (version 1.1). Eur J Cancer.2009 ;45:228-47; RANO Criteria for High-Grade Gliomas, Source: Wen PY, Macdonald DR, Reardon DA, Cloughesy TF, en AG, Galanis E, Degroot J, Wick W, t MR, n AB, Tsien C, Mikkelsen T, Wong ET, Chamberlain MC, Stupp R, Lamborn KR, Vogelbaum MA, van den Bent MJ, Chang SM. Updated response assessment criteria for high-grade gliomas: response assessment in neuro-oncology g group. J Clin Oncol. 2010;28(11):1963-72; CA-125 Rustin ia for Ovarian Cancer Response, Source: Rustin GJ, Quinn M, Thigpen T, du Bois A, Pujade-Lauraine E, Jakobsen A, Eisenhauer E, Sagae S, Greven K, Vergote I, Cervantes A, Vermorken J. Re: New guidelines to evaluate the response to treatment in solid tumors (ovarian cancer). J Natl Cancer Inst. 2004; 96(6):487-8; PSA Working Group 2 Criteria for Prostate Cancer Response, Source: Scher HI, Halabi S, Tannock I, Morris M, Sternberg CN, Carducci MA, erger MA, Higano C, Bubley GJ, Dreicer R, Petrylak D, Kantoff P, Basch E, Kelly WK, Figg WD, Small EJ, Beer TM, Wilding G, Martin A, Hussain M; Prostate Cancer Clinical Trials Working Group. Design and end points of clinical trials for patients with progressive prostate cancer and castrate levels of testosterone: recommendations of the Prostate Cancer al Trials Working Group. J Clin Oncol. 2008;26(7):1148-59.

Resistance is associated with there not being an observable and/or measurable reduction in, or absence of, one or more of the following: reduction in the number of abnormal cells, ably cancerous cells, or absence of the abnormal cells, preferably cancerous cells; for cancerous diseases: reduction in tumour size; inhibition (i.e., slowed to some extent and preferably stopped) of further tumour growth; reduction in the levels of tumour markers such as PSA and CA-125; tion (i.e., slowed to some extent and preferably stopped) of cancer cell infiltration into other organs (including the spread of cancer into soft tissue and bone); inhibition (i.e., slowed to some extent and preferably stopped) of tumour metastasis; alleviation of one or more of the symptoms associated with the specific cancer; and reduced ity and mortality.

In a preferred embodiment resistance means there is no observable and/or measurable reduction in, or absence of, one or more of the following criteria: ion in tumour size; inhibition of further tumour growth; inhibition of cancer cell infiltration into other organs; and inhibition of tumour metastasis.

In a more preferred ment resistance refers to one or more of the following ia: no reduction in tumour size; no tion of further tumour growth, no tion of cancer cell infiltration into other organs; and no inhibition of tumour metastasis.

Measurement of the aforementioned resistance criteria is according to clinical guidelines well known to a person in the field of cancer treatment, such as those listed above for measuring the response of a cancerous disease.

Response may also be established in vitro by assessing cell proliferation and/or cell death. For example, effects on cell death or proliferation may be assessed in vitro by one or more of the following well established : A) Nuclear staining with Hoechst 33342 dye providing information about nuclear morphology and DNA fragmentation which are hallmarks of apoptosis. B) n V binding assay which reflects the phosphatidylserine content of the outer lipid bilayer of the plasma membrane. This event is considered an early hallmark of apoptosis. C) TUNEL assay (Terminal deoxynucleotidyl transferase mediated dUTP Nick End Labeling assay), a fluorescence method for evaluating cells undergoing apoptosis or necrosis by measuring DNA fragmentation by labeling the terminal end of nucleic acids. D) MTS proliferation assay measuring the lic activity of cells. Viable cells are metabolically active s cells with a compromised respiratory chain show a reduced activity in this test. E) Crystal violet staining assay, where effects on cell number are red through direct staining of cellular components. F) Proliferation assay monitoring DNA synthesis through incorporation of bromodeoxyuridine (BrdU). tory effects on growth/proliferation can be ly determined. G) YO-PRO assay which es a membrane impermeable, fluorescent, monomeric cyanine, nucleic acid stain, which permits analysis of dying (e.g. tic) cells without interfering with cell viability. Overall s on cell number can also be analysed after cell permeabilisation. H) Propidium iodide staining for cell cycle distribution which shows alterations in distribution among the ent phases of the cell cycle. Cell cycle ing points can be determined. I) Anchorage-independent growth assays, such as colony outgrowth assays which assess the ability of single cell suspensions to grow into colonies in soft agar.

In a preferred embodiment relating to ination of resistance in vitro, resistance means there is no decrease in the proliferation rate of al cells and/or reduction in the number of abnormal cells. More preferably resistance means there is no decrease in the proliferation rate of cancerous cells and/or no reduction in the number of cancerous cells. The reduction in the number of abnormal, preferably cancerous, cells may occur h a variety of programmed and non-programmed cell death mechanisms. Apoptosis, caspase-independent programmed cell death and autophagic cell death are examples of programmed cell death. However the cell death ia involved in embodiments of the invention are not to be taken as limited to any one cell death mechanism. phospho-Akt As defined above, the term Akt is used herein to encompass all the previously mentioned synonyms and isoforms and phospho-Akt is Akt that has been phosphorylated on one or more residues, with the proviso that for Akt1, Akt2, and Akt3 the designation phospho-Akt is used to indicate phosphorylation at a site other than T308, T309 or T305 respectively. To clarify this r, the phospho-Akt may or may not be phosphorylated at T308, T309 or T305 for Akt 1, Akt 2 and Akt3 respectively, but the ation phospho-Akt indicates phosphorylation at sites other than these.

Preferred es of the protein sequence of Akt (human Akt) are listed in SEQ. ID No. 1 to 3, Figures 9 - 11. However the term Akt also asses homologues, mutant forms, allelic variants, isoforms, splice variants and equivalents of these sequences. The human homologues, mutant forms, allelic variants, isoforms, splice variants and equivalents of these sequences are more preferred embodiments. More preferably it encompasses sequences having at least about 75% identity, especially preferably at least about 85% identity, particularly preferably at least about 95% identity, to any of the sequences ented by SEQ. ID. No. 1 to 3. In an especially red embodiment, Akt corresponds to any of the sequences represented by SEQ ID NO. 1 to 3 and sequences having at least 99% identity with any of these sequences. In a particularly preferred embodiment, Akt corresponds to the sequence represented by SEQ ID NO. 1 and ces having at least 95% identity with this sequence, preferably at least 99% identity. In a more particularly preferred embodiment, Akt corresponds to a sequence ented by any of SEQ ID NO. 1 to 3. In a still more particularly preferred embodiment Akt corresponds to a sequence represented by SEQ ID NO. 1.

In a particularly preferred embodiment, phospho-Akt shall refer to Akt in the preferred embodiments thereof are as given in the preceding paragraph) that has been phosphorylated on the following serine residue: for Akt1 (SEQ. ID. No.1): S473; for Akt2 (SEQ. ID. No. 2): S474; and for Akt3 (SEQ. ID. No. 3): S472.

In yet another ally preferred embodiment, phospho-Akt corresponds to any of the ces represented by SEQ ID NO. 1 to 3 and sequences having at least 99% identity with any of these ces, and wherein for the sequence represented by SEQ. ID. No.1, S473 is phosphorylated, or for the sequence represented by SEQ. ID. No.2, S474 is phosphorylated, or for the sequence represented by SEQ. ID. No.3, S472 is phosphorylated.

In a very particularly preferred embodiment phospho-Akt corresponds to a ce represented by SEQ ID NO. 1 that has been phosphorylated on S473.

Level of phospho-Akt The level of phospho-Akt may be assayed in the sample by technical means well known to a skilled person. Examples of methods of protein sion analysis known in the art which are suitable to measure the level of phospho-Akt at the protein level e, but are not limited to, i) immunohistochemistry (IHC) analysis, ii) western ng iii) immunoprecipitation iv) enzyme linked sorbant assay (ELISA), v) radioimmunoassay, vi) Fluorescence activated cell sorting (FACS), vii) mass spectrometry, including matrix assisted laser desorption/ionization (MALDI, e.g.

MALDI-MS) or electrospray (e.g. ESI-MS).

The antibodies involved in some of the above methods may be monoclonal or polyclonal antibodies, dy fragments, and/or various types of synthetic antibodies, including chimeric antibodies. The antibody may be labeled to enable it to be detected or capable of detection following reaction with one or more further species, for e using a secondary antibody that is labeled or capable of producing a detectable result. Antibodies specific to phospho-Akt are available cially from Cell Signaling or can be prepared via conventional dy generation methods well known to a skilled person.

Preferred methods of n analysis are ELISA, mass ometry ques, immunohistochemistry and western blotting, more preferably ELISA, western blotting and immunohistochemistry, particularly preferably western blotting and immunohistochemistry. In western blotting, also known as immunoblotting, labelled antibodies may be used to assess levels of protein, where the intensity of the signal from the detectable label corresponds to the amount of protein, and can be quantified for example by densitometry.

Immunohistochemistry again uses labelled antibodies to detect the presence and relative amount of the biomarker. It can be used to assess the percentage of cells for which the biomarker is present. It can also be used to assess the localisation or ve amount of the biomarker in individual cells; the latter is seen as a function of the intensity of staining.

ELISA stands for enzyme linked immunosorbant assay, since it uses an enzyme linked to an antibody or antigen for the detection of a specific protein. ELISA is typically performed as follows (although other variations in ology exist): a solid substrate such as a 96 well plate is coated with a primary dy, which recognises the biomarker. The bound biomarker is then recognised by a secondary antibody specific for the biomarker. This may be directly joined to an enzyme or a third anti-immunoglobulin antibody may be used which is joined to an enzyme. A substrate is added and the enzyme catalyses a reaction, yielding a ic colour.

By measuring the optical density of this colour, the presence and amount of the biomarker can be determined.

Uses of biomarker The biomarker may be used to predict inherent resistance of the disease in a subject to the compound of general formula I or a ceutically acceptable tive thereof as defined above.

The biomarker may be used to select subjects suffering or predisposed to suffering from a disease, preferably cancer, for treatment with a compound of general formula I or a pharmaceutically acceptable tive thereof as defined above. The levels of such a biomarker may be used to identify subjects likely to respond or to not respond to treatment with such agents. fication of subjects may be made in order to avoid ssary treatment regimes. In ular the biomarker may be used to identify subjects from whom a sample or samples do not display a higher level of phospho-Akt, relative to a standard level or set of standard levels, whereupon such subjects may then be selected for treatment with the compound of formula I or a pharmaceutically acceptable derivative thereof as defined above.

The biomarker may also be used to assist in the determination of treatment regimes, regarding amounts and schedules of dosing. Additionally, the biomarker may be used to assist in the selection of a combination of drugs to be given to a subject, including a compound or compounds of general formula I or a pharmaceutically acceptable derivative thereof, and another chemotherapeutic (cytotoxic) agent or agents. Furthermore, the biomarker may be used to assist in the determination of therapy strategies in a subject including whether a compound of general formula I or a pharmaceutically acceptable derivative thereof is to be stered in combination with targeted therapy, endocrine therapy, radiotherapy, immunotherapy or surgical intervention, or a combination of these.

Phospho-Akt may also be used in combination with other biomarkers to predict the se to a compound of general formula I or a pharmaceutically acceptable derivative thereof and to determine treatment s. It may rmore be used in combination with chemo-sensitivity g to t resistance and to determine treatment s. Chemo-sensitivity testing involves directly applying a compound of general a I to cells taken from the subject, for example from a subject with haematological malignancies or accessible solid tumours, for example , head and neck cancers or melanomas, to ine the response of the cells to the nd.

Method of treatment Also described is a method of treatment and phospho-Akt for use in a method of treatment, wherein the level of phospho-Akt is first established relative to a standard level or set of standard levels and then a compound of general formula I or a pharmaceutically acceptable derivative thereof as defined above, is administered if the level of phospho-Akt in said sample is not higher than a standard value or set of standard values. The compound of formula I or a pharmaceutically acceptable derivative thereof may be administered in a pharmaceutical composition, as is well known to a person skilled in the art. Suitable compositions and dosages are for example disclosed in WO 03994 A1 pages 35-39, which are specifically incorporated by reference herein. Compositions for enteral stration, such as nasal, buccal, rectal or, especially, oral stration, and for parenteral administration, such as intravenous, intramuscular or subcutaneous administration, to warm-blooded animals, especially humans, are especially preferred. More ularly, compositions for intravenous administration are preferred.

The compositions comprise the active ingredient and a pharmaceutically acceptable carrier. An e of a composition includes, but is not d to, the following: 5000 soft n capsules, each comprising as active ingredient 0.05 g of one of the compounds of general formula (I), are prepared as follows: 250 g pulverized active ingredient is suspended in 2 liter Lauroglykol lene glycol laurate, Gattefossé S.A., Saint Priest, ) and ground in a wet pulverizer to produce a le size of about 1 to 3 µm. 0.419 g portions of the mixture are then uced into soft gelatin es using a capsule-filling machine.

Also described is a method of treating a neoplastic or autoimmune disease, preferably cancer, by first decreasing the level of phospho-Akt in a t that has a sample with a higher level of phospho-Akt compared to a standard level or set of standard levels, then treating the subject with a compound of general formula I or a pharmaceutically acceptable derivative as defined above. The level of phospho-Akt may be sed by direct or indirect chemical or genetic means. Examples of such methods are treatment with a drug that results in reduced phospho-Akt levels, targeted delivery of viral, plasmid or peptide constructs or antibody or siRNA or antisense to downregulate the level of phospho-Akt. For example siRNA may be used to reduce the level of rictor expressed, thus reducing mTORC2 complex formation and activity, and thereby indirectly lower the level of phosphorylated Akt.

The subject may then be treated with a compound of general formula I or a pharmaceutically able derivative thereof.

A compound of l formula I or a pharmaceutically acceptable derivative thereof can be administered alone or in combination with one or more other therapeutic agents. Possible combination therapy may take the form of fixed combinations, or the administration of a compound as described and one or more other therapeutic agents which are staggered or given independently of one r, or the combined administration of fixed combinations and one or more other therapeutic agents. A nd of general formula I or a pharmaceutically acceptable derivative thereof can, besides or in addition, be administered especially for tumour therapy in combination with chemotherapy (cytotoxic), targeted therapy, endocrine therapy, herapy, immunotherapy, surgical ention, or a combination of these. Long-term therapy is equally possible as is adjuvant therapy in the context of other treatment strategies, as bed above. Other possible treatments are y to maintain the patient's status after tumour regression, or even chemo-preventive therapy, for e in patients at risk.

Kit and device Also described is a kit, and in another aspect to a device, for predicting the response, preferably of a disease in a subject, to a nd of general formula I or a pharmaceutically acceptable derivative thereof as defined above, comprising reagents necessary for measuring the level of phospho-Akt in a sample. Preferably, the reagents comprise a capture reagent comprising a detector for phospho-Akt and a detector reagent.

The kit and device may also preferably comprise a comparator module which ses a standard value or set of standard values to which the level of phospho-Akt in the sample is compared. In a preferred ment, the comparator module is included in instructions for use of the kit. In another preferred embodiment the comparator module is in the form of a display device, for example a strip of colour or numerically coded material which is designed to be placed next to the readout of the sample measurement to indicate ance levels. The standard value or set of standard values may be determined as bed above.

The reagents are preferably antibodies or antibody fragments which selectively bind to phospho-Akt. These may for example be in the form of one specific primary antibody which binds to phospho-Akt and a secondary antibody which binds to the primary antibody, and which is itself labelled for detection. The primary antibody may also be labelled for direct detection. The kits or devices may optionally also n a wash solution(s) that selectively allows retention of the bound biomarker to the capture reagent as compared with other biomarkers after washing. Such kits can then be used in ELISA, n blotting, flow cytometry, immunohistochemical or other immunochemical methods to detect the level of the biomarker.

Furthermore the device may comprise imaging devices or measurement devices (for example, but not restricted to, measurement of fluorescence) which further process the measured signals and transfer them into a scale in a comparator module.

More preferably the kit comprises a compound of general formula I, or a pharmaceutically acceptable derivative thereof as d above. This compound may then be administered to the subject, in accordance with the level of the ker in the sample from the subject, as measured by the reagents sed in the kit. Therefore the kit according to the invention may be used in the method of treatment, as defined above. In an especially preferred embodiment the kit comprises a compound of the following a or a ceutically acceptable salt thereof N N N N O In a particularly preferred embodiment of the kit the pharmaceutically acceptable salt is a ochloride salt. Also described is the use of such a kit as described above.

In the present specification the words “comprise” or ises” or “comprising” are to be understood as to imply the inclusion of a stated item or group of items, but not the exclusion of any other item or group of items.

Experimental methodology fluorescent ng of cultured cells A549 human non-small cell lung cancer (NSCLC, ATCC reference number CCL-185) cells, HeLa cervical cancer cells (ATCC reference number CCL-2) and SKBR3 breast carcinoma cells (ATCC reference number HTB-30) were seeded at densities of 50% on round microscope coverslips and cultured for 24 hours in RPMI- 1640 containing 10 % FCS (also referred to as FBS) at 37°C, 5% CO2. Compounds to be tested were dissolved in DMSO. The cell culture medium was replaced with medium containing the diluted compound(s) taxel, vinblastine, colchicine and nocodazole were purchased from Sigma-Aldrich) or vehicle. After treatment for the times indicated in the Brief Description of the Figures, coverslips were washed and cells were fixed in methanol/acetone (1:1) for 5 minutes at room temperature and subsequently ted in blocking buffer (0.5% BSA and 0.1% TX-100 in PBS) for minutes at room temperature. Specimens were then ted with anti-alphatubulin dy (Sigma, 1:2000) for 1 hour at room temperature in blocking .

After several washing steps cells were incubated with AlexaFluor-488 goat-anti- mouse IgG (Molecular Probes, 1:3000) for 1 hour at room temperature followed by several washing steps with blocking buffer. Specimens were then mounted with ProLong Gold antifade (Molecular Probes), sealed with nail polish and examined with a Leica immunofluorescence microscope. Images were captured with a cooled CCD- camera and processed by ImageJ software.

Colony Outgrowth Assay: Single cell suspensions of patient-derived tumour xenografts (maintained in nude mice) were prepared. For colony outgrowth assays, cells were plated in soft agar in 24-well plates according to the assay introduced by Hamburger & Salmon ry bioassay of human tumour stem cells, Science , 1977,197:461-463). 2x104- 6x10 4 cells in 0.2 mL medium containing 0.4 % agar were plated out on a bottom layer of 0.75 % agar. Test compounds were d in 0.2 mL culture medium. Every 24-well plate contained untreated controls and samples in triplicates. es were incubated at 37°C and 7.5 % CO2 for 5 - 28 days. 24 hours prior to analysis, vital colonies were stained with a solution of metabolizable tetrazolium salt (Alley MC et al, Life Sci. 1982, 31:3071-3078) and were counted with an automatic image analysis system (Omnicon 3600, Biosys GmbH).

Relative drug effects were expressed by the ratio of the mean number of colonies in the treated wells and the control wells. IC70 -values were determined by plotting compound concentrations versus ve colony counts.

Protein Extraction Tumours were extracted in ice-cold buffer ning 50 mM HEPES (pH 7.5), 150 mM NaCl, 25 mM β-glycerophosphate, 25 mM NaF, 5 mM EGTA, 1 mM EDTA, 0.1% NP40, 15 mM pyrophosphate, 2 mM sodium orthovanadate, 10 mM sodium molybdate, leupeptin (10 µg/mL), aprotinin (10 µg/mL) and 1 mM PMSF (1 mL extraction volume per 45 mg tumour). After homogenisation by Polytron, lysates were adjusted to 1% NP40 and incubated on ice for 20 min. Lysates were clarified by centrifugation and frozen at -80°C.

Immunoblotting/Western blotting Immunoblotting was performed using 20 µg of total protein per lane. Protein concentration was determined with the BCA Protein Assay (Pierce). Protein was separated on a 10% SDS-gel and transferred to a PVDF membrane using Wet Blotting (45 min, 250 mA/gel). The primary dies used for immunoblotting were as follows: Phospho-Akt (serine 473) (available from Cell Signalling, reference number 9271) origin: rabbit polyclonal antibody, dilution 1:1000, buffer conditions: PBS containing 0.5 % milk / 0.1 % tween; Akt protein (available from Epitomics, reference number ) origin: rabbit monoclonal antibody, dilution 1:2000, buffer ions: PBS containing 0.5 % milk / 0.1 % tween; Actin: (available from Chemicon, nce number MAB1501) origin: mouse, monoclonal, dilution , buffer conditions: PBS containing 0.5 % milk / 0.1 % tween.

The secondary antibodies used for immunoblotting were peroxidaseconjugated goat anti-rabbit or goat anti-mouse (available from Jackson ImmunoResearch Laboratories INC: reference number 111144 JIR and 115- 035-146 JIR), dilution 1:5000, buffer conditions: 0.5% milk in PBS/0.1% Tween.

Labelled bands were revealed using a t Stella 3200 High Performance Imaging System.

Immunohistochemistry Fixation of patient-derived tumour xenografts (maintained in nude mice) was performed in 10 % neutral-buffered formalin containing 4 % formaldehyde for 20 – 28 hours at room temperature. Fixed specimens were kept in a solution of 70 % ethanol for a maximum of one week prior to dehydration and paraffin embedding ing to a standard procedure, using the conditions listed below: Sequential Treatment time (hours) 70% EtOH 1 80% EtOH 2 99% EtOH 1 100% panol 0.5 100% Isopropanol 1 Xylol 0.5 Xylol 1 Xylol 1 in 1 Paraffin 2 Paraffin 2 Paraffin sections of approximately 2 µm were cut and processed by using the automated immunostainer Benchmark XT® ) running the standard processing steps. The visualisation of the specific antibody staining was done with DAB (3,3-diaminobenzidine) as genic substrate at a concentration of 5 mg/mL. The following primary dy and processing conditions were used for staining: Antibody Specification Processing Anti-Akt-pS473 from Dako, # M3628 MTec100/30: EDTA-citrate buffer retrieval rabbit monoclonal antibody pH 8, 100°C for 30 minutes. Antibody incubation at 37°C for 32 minutes at a dilution of 1:20 Detailed examples Example 1: A distinct mitotic phenotype induced by nds of general formula I Treatment with compound A (BAL27862) or with compound B, or compound C induced a highly reproducible and distinct microtubule phenotype in all tumour cell lines tested (shown for BAL27862 in A549, HeLa and SKBR3 cells in Figure 1, and for compound C and compound B in A549 cells in Figure 2). In dividing cells an apparent fragmentation of the mitotic spindle occurred, resulting in the formation of dot-like structures (Figure 1). This phenotype was shown to be distinct from that observed with conventional microtubule targeting agents, such as the microtubule stabiliser paclitaxel and the microtubule destabilisers vinblastine and colchicine (Figure 3) and nocodazole (Figure 4).

Example 2: BAL27862 mes microtubule phenotype induced by conventional microtubule-targeting drugs in a dominant fashion In order to show the uniqueness of its activity on ubules, BAL27862 was tested in combination with vinblastine, colchicine and axel (Figure 5) and zole (Figure 6) using A549 cells. Treatment with vinblastine, colchicine, paclitaxel or nocodazole alone induced the c microtubule ypes characteristic of these agents. However, combination treatment with BAL27862 for the last 4 hours resulted in disruption of the microtubule ures; creating a phenotype consistent with treatment of BAL27862 alone, despite the continued presence of stine, colchicine, paclitaxel or nocodazole. In contrast, treating first with BAL27862 and subsequently for 4 hours in combination with vinblastine, cine, paclitaxel or nocodazole had no impact on the observed microtubule ype that was consistent with treatment with BAL27862.

These data trate that nds of formula I affect microtubule biology consistently, but in a different manner than conventional microtubule targeting agents.

Detailed Examples according to the invention Example 3: Association of high phospho-Akt expression levels with patientderived tumour cells resistant to BAL27862 treatment.

Based on colony outgrowth assays, using tumour cells derived from 8 patient-derived tumours maintained as xenografts in mice, BAL27862-sensitive or resistant tumour cells were identified from gastric cancer, colorectal cancer, melanoma, and lung cancer (see Table 1). Concentrations at which 70% growth inhibition was observed versus controls (IC70) are shown in Table 1. In this table, BAL27862-sensitive tumour cells were those that had IC70 values in the low nanomolar range, while BAL27862-resistant tumour cells had IC70 values >600 nanomolar. Paclitaxel and vinblastine data, using the same ex vivo assay, was ble for 7 of the 8 tumour models. Of these 7 , all were resistant to treatment with paclitaxel, while 6 were sensitive to treatment with vinblastine.

Table 1 Cancer name se to IC70 BAL27862 Response to Response to type BAL27862 [micromolar] paclitaxel stine Gastric GFX 251 sensitive 0.485 resistant sensitive c GFX 97 resistant >3.5 resistant sensitive Lung LXFE 211 sensitive 0.021 resistant sensitive Lung LXFE 397 resistant >3.5 Not known Not known Melanoma MEXF 1341 sensitive 0.025 resistant ive Melanoma MEXF 276 resistant >3.5 resistant sensitive Colorectal CXF 1103 sensitive 0.022 resistant resistant cancer Colorectal CXF 243 resistant 0.696 resistant sensitive cancer Immunoblotting analysis was performed in order to measure the levels of o- Akt (using an antibody recognising phosphorylated serine-473) and Akt protein in the same tumours maintained as xenografts. The actin levels were included on the immunoblot as a loading control.

Analysis of phospho-Akt levels indicated that phospho-Akt levels varied across the tumours (Figure 7).

Based on the the colony outgrowth assay and the same IC70 criteria, there was no ation between paclitaxel or vinblastine resistance and high phospho- Akt expression levels (compare Figure 7 with Table 1). This is evident, for example, in the gastric cancer models. Although GXF 251 and GXF 97 were both ant to paclitaxel, for GXF 251 the phospho-Akt levels were virtually undetectable, while for GXF 97 the levels were clearly higher. The same lack of association was true for the vinca alkaloid, vinblastine, in the gastric models, since both these tumours were sensitive to vinblastine. This lack of ation was repeated in the melanoma and colorectal cancer . Thus phospho-Akt levels were shown to be unsuitable as a reliable biomarker of resistance to the tional microtubule agents paclitaxel and vinblastine in patient-derived tumour models.

Surprisingly, in contrast, when the BAL27862 ance data, as defined by the colony outgrowth assay, was compared with the phospho-Akt level, phospho-Akt sion was shown to be higher only in the resistant tumours and not in the ive tumours derived from the same tumour histotype (compare Figure 7 with Table 1). Increased expression levels were therefore consistently indicative of resistance to BAL27862. Thus phospho-Akt levels were shown to be a biomarker of resistance for the compound described herein, BAL27862.

Example 4: Immunohistochemical analysis of gastric tumour xenografts Immunohistocehmical analysis was performed on the gastric tumour xenografts (Figure 8), revealing a higher level of o-Akt in the tumour model GXF 97. Again a clear correlation was seen between higher levels of phospho-Akt and resistance to BAL27862 (tumour model GXF 97 was 62-resistant, while tumour model GXF 251 was BAL27862-sensitive; as defined by the colony outgrowth assay – Table 1). Thus phospho-Akt levels were again shown to be a biomarker of ance for the compound described , BAL27862.

List of abbreviations A549 human non-small cell lung cancer cell line BCA bicinchoninic acid BrdU bromodeoxyuridine BSA bovine serum albumin CA-125 cancer antigen 125 CCD -coupled device CREST limited scleroderma syndrome DAB 3,3-diaminobenzidine DMSO dimethylsulphoxide DNA deoxyribonucleic acid dUTP 2´-Deoxyuridine 5´-Triphosphate EDTA ethylenediaminetetraacetic acid EGTA ethyleneglycol-bis (β-aminoethyl)-N,N,N ′,N ′-tetraacetic acid ELISA enzyme-linked immunosorbent assay ESI-MS electrospray tion mass spectrometry EtOH ethanol FACS fluorescence activated cell scan/sorting FCS/FBS foetal calf / foetal bovine serum G2/M transition from G2 to the mitotic phase in the cell cycle HeLa human squamous cell cancer cell line HEPES 4-(2-Hydroxyethyl)piperazineethanesulphonic acid Hoe33342 2'-(4'-Ethoxyphenyl)(4-methylpiperazinyl)-2,5'-bis-1H- benzimidazole trihydrochloride trihydrate IC 70 concentration at which 70% signal is inhibited IgG immunoglobulin G IHC immunohistochemistry ISET isolation by size of epithelial tumor cells MALDI matrix-assisted-laser-desorption/ionisation mass-spectrometry mTORC2 mammalian target of rapamycin complex2 MTS 3-(4,5-dimethylthiazolyl)(3-carboxymethoxyphenyl)(4- sulphophenyl)-2H-tetrazolium NaF sodium fluoride NCBI al Center for Biotechnology Information NSCLC all cell lung cancer NP40 t P40 PBS phosphate buffered saline P-gp P-glycoprotein PKB protein kinase B PMSF phenylmethylsulphonyl fluoride PSA prostate-specific antigen PVDF polyvinylidene fluoride RAC related to A and C kinases RANO response assessment for high-grade gliomas RECIST response evaluation criteria in solid tumors RICTOR cin-insensitive companion of mTOR RPMI-1640 cell culture medium used for culturing transformed and nontransformed otic cells and cell lines SDS sodium dodecyl sulphate SEQ. ID No. sequence fication number siRNA small inhibitory ribonucleic acid SKBR3 human mammary carcinoma cell line TUNEL terminal deoxynucleotidyl transferase dUTP nick end labeling Tween non-ionic detergent YO-PRO fluorescent, monomeric cyanine, c acid stain The term ‘comprising’ as used in this specification and claims means ‘consisting at least in part of’. When interpreting ents in this specification and claims which es the ‘comprising’, other features besides the features prefaced by this term in each statement can also be present. Related terms such as ‘comprise’ and ‘comprised’ are to be interpreted in similar manner.

In this specification where reference has been made to patent specifications, other external documents, or other sources of information, this is lly for the purpose of providing a context for discussing the features of the invention. Unless specifically stated otherwise, reference to such external documents is not to be construed as an admission that such documents, or such sources of information, in any jurisdiction, are prior art, or form part of the common general knowledge in the art.

Claims (30)

We claim:

1. Use of phospho-Akt as a biomarker for predicting the response to a compound, wherein phospho-Akt is Akt that has been phosphorylated on one or more es, with the proviso that for Akt1, Akt2, and Akt3 the ation 5 o-Akt is used to indicate phosphorylation at a site other than T308, T309 or T305 respectively, wherein the compound is a compound of general formula I, R3 R2 N R4 N R5 N N wherein 10 R represents phenyl, thienyl or pyridinyl wherein phenyl is optionally substituted by one or two substituents independently selected from alkyl, ower alkyl, hydroxy-lower alkyl, lower alkoxy-lower alkyl, acyloxy-lower alkyl, phenyl, hydroxy, lower alkoxy, hydroxy-lower alkoxy, lower alkoxy-lower alkoxy, phenyl-lower alkoxy, lower alkylcarbonyloxy, amino, 15 monoalkylamino, dialkylamino, lower alkoxycarbonylamino, lower alkylcarbonylamino, substituted amino wherein the two substituents on nitrogen form er with the en heterocyclyl, lower alkylcarbonyl, carboxy, lower alkoxycarbonyl, cyano, halogen, and nitro; and wherein two adjacent substituents are methylenedioxy; 20 and wherein nyl is optionally substituted by lower alkoxy, amino or halogen; X represents a group C=Y, wherein Y stands for oxygen or nitrogen substituted by hydroxy or lower alkoxy; R1 represents hydrogen, lower alkylcarbonyl, hydroxy-lower alkyl or cyano-lower alkyl; R2, R3 and R6 ent hydrogen; R4 and R5, independently of each other, ent hydrogen, lower alkyl or lower 5 alkoxy; or R4 and R5 together represent methylenedioxy; and pharmaceutically acceptable salts, solvates, esters and amides of lly occurring amino acids, small peptides or pegylated y acids, and salts of such 10 esters and amides thereof; or wherein R represents phenyl or pyridinyl wherein phenyl is optionally substituted by one or two tuents independently 15 selected from alkyl, halo-lower alkyl, hydroxy-lower alkyl, lower alkoxy-lower alkyl, y-lower alkyl, phenyl, hydroxy, lower alkoxy, hydroxy-lower alkoxy, lower alkoxy-lower alkoxy, phenyl-lower alkoxy, lower alkylcarbonyloxy, amino, monoalkylamino, dialkylamino, lower alkoxycarbonylamino, lower alkylcarbonylamino, substituted amino wherein the two substituents on en form 20 together with the nitrogen heterocyclyl, lower alkylcarbonyl, carboxy, lower alkoxycarbonyl, formyl, cyano, halogen, and nitro; and wherein two adjacent substituents are methylenedioxy; and wherein pyridinyl is optionally substituted by lower , amino or halogen; 25 X represents oxygen; R1 represents hydrogen, lower alkylcarbonyl, hydroxy-lower alkyl or cyano-lower alkyl; R2, R3 and R6 represent hydrogen; 30 R4 and R5, independently of each other, represent hydrogen, lower alkyl or lower alkoxy; or R4 and R5 together represent methylenedioxy; and pharmaceutically acceptable salts, solvates, esters and amides of naturally occurring amino acids, small peptides or pegylated hydroxy acids, and salts of such esters and amides thereof, and wherein the prefix lower s a l having up to and including a m of 7 5 carbon atoms, and wherein the response is of a disease in a subject and the biomarker phospho-Akt is measured ex vivo in a sample or samples taken from the human or animal body.

2. Use according to claim 1, wherein the sample or samples are taken from 10 the human body.

3. Use according to claim 1 or claim 2, wherein in the compound of general formula I R represents phenyl or pyridinyl 15 wherein phenyl is optionally substituted by one or two substituents independently selected from lower alkyl, lower , amino, acetylamino, halogen and nitro; and wherein pyridinyl is optionally substituted by amino or halogen; X represents a group C=O; R1 represents hydrogen or cyano-lower alkyl; R2, R3, R4, R5 and R6 represent en; and pharmaceutically acceptable salts, solvates, esters and amides of lly 25 occurring amino acids, small peptides or pegylated hydroxy acids, and salts of such esters and amides thereof, and wherein the prefix lower denotes a radical having up to and including a maximum of 7 carbon atoms.

4. Use according to any one of claims 1 to 3, wherein the compound is represented by the following formula N N wherein R, Y and R1 are defined as follows: R Y R1 O CH2CH2CN O H O CH2CH2CN NH N or pharmaceutically acceptable salts, solvates, esters and amides of naturally ing amino acids, small peptides or pegylated hydroxy acids, and salts of such esters and amides thereof.

5. Use according to any one of claims 1 to 4, n the compound is N N N N O or pharmaceutically able salts, solvates, esters and amides of naturally occurring amino acids, small peptides or pegylated hydroxy acids, and salts of such esters and amides thereof. 5

6. Use according to any one of claims 1 to 5, wherein the prefix lower s a l having up to and including a maximum of 4 carbon atoms.

7. Use according to any one of claims 1 to 6, wherein an amide of the compound of formula I with glycine, alanine or lysine is used as a prodrug, the amide being formed from an amino group present within the R group of the compound of 10 general formula I as defined in any one of claims 1 to 6 and the carboxy group of glycine, alanine or lysine.

8. Use according to any one of claims 1 to 7, wherein the compound is N N N N O or a pharmaceutically acceptable salt thereof. 5

9. Use ing to claim 8, wherein the pharmaceutically acceptable salt is a hydrochloride salt or dihydrochloride salt.

10. Use according to any one of claims 1 to 9, for predicting the resistance of a disease in a subject to said compound.

11. Use according to any one of claims 1 to 10, wherein phospho-Akt is Akt 10 that has been phosphorylated on the following serine residue: for Akt1: S473; for Akt2: S474; and for Akt3: S472. 15

12. Use according to any one of claims 1 to 11, wherein the disease is a stic disease or mune disease.

13. Use according to claim 12, wherein the disease is cancer.

14. Use according to any one of claims 1 to 13, wherein the e is 20 selected from the group consisting of breast cancer, prostate cancer, al cancer, ovarian cancer, gastric cancer, colorectal cancer, pancreatic cancer, liver cancer, brain cancer, neuroendocrine cancer, lung cancer, kidney cancer, logical malignancies, melanoma and sarcomas.

15. Use according to claim 14, wherein the cancer is selected from the group consisting of breast cancer, cervical cancer, gastric cancer, lung cancer, colorectal 5 cancer and melanoma.

16. Use according to claim 15, wherein the cancer is selected from the group consisting of gastric cancer, colorectal cancer, lung cancer and ma.

17. Use according to any one of claims 1 to 16, wherein a higher level of phospho-Akt in the sample from the subject ve to a standard value or set of 10 standard values ts resistance.

18. Use according to claim 17, wherein higher phospho-Akt levels in a sample or samples i) relative to a standard value or set of standard values from ts with the same tumour histotype; or 15 ii) relative to a standard value or set of standard values from normal tissue; are predictive of resistance.

19. Use according to any one of claims 1 to 18, wherein the biomarker is used to select subjects suffering or posed to suffering from a disease, for treatment with a compound of l formula I or a pharmaceutically able 20 salt, solvate, ester or amide of a naturally occurring amino acid, small peptide or pegylated hydroxy acid, or a salt of such ester or amide thereof as defined in any one of claims 1 to 9.

20. Use according to claim 19, wherein the disease is cancer.

21. Use according to any one of claims 1 to 20, wherein the sample is derived from tumour tissue, normal tissue, cell lines or ating tumour cells.

22. Use ing to claim 21, wherein the sample is d from tumour tissue.

23. A method for predicting in a subject suffering from cancer the response 5 of a disease that cancer to a compound of general formula I or a pharmaceutically acceptable salt, solvate, ester or amide of a naturally occurring amino acid, small peptide or pegylated hydroxy acid, or a salt of such ester or amide thereof as defined in any one of claims 1 to 9, comprising the steps of: a) measuring ex vivo a level of phospho-Akt, as defined in claim 1 or claim 10 11, in a sample pre-obtained from tumour tissue or circulating tumour cells of the subject to obtain a value or values representing this level; and b) comparing the value or values from step a) to a standard value or set of rd values from subjects with the same cancer type, wherein a higher level of phospho-Akt in the sample relative to the standard 15 value or set of standard value is predictive of resistance of the subject's cancer to the compound of formula (I) or pharmaceutically acceptable salt, solvate, ester or amide of a naturally occurring amino acid, small e or pegylated hydroxy acid, or a salt of such ester or amide thereof.

24. Use of a compound of general formula I or of a pharmaceutically 20 acceptable salt, solvate, ester or amide of a naturally ing amino acid, small e or pegylated hydroxy acid, or a salt of such ester or amide thereof as defined in any one of claims 1 to 9, for the preparation of a pharmaceutical composition for treating a cancer in a subject in need f, wherein the subject is selected for treatment with the compound of general formula I or with the pharmaceutically 25 acceptable salt, solvate, ester or amide of a naturally occurring amino acid, small e or pegylated hydroxy acid, or a salt of such ester or amide thereof as defined in any one of claims 1 to 9, if the level of the phospho-Akt defined in claim 1 or claim 11, measured ex vivo in a sample taken from the subject, is not higher than a standard value or set of standard values from subjects with the same tumour ype or from normal cells, tissue or body fluid.

25. A kit when used for predicting the response to a compound of general formula I or of a pharmaceutically acceptable salt, solvate, ester or amide of a 5 naturally occurring amino acid, small peptide or pegylated hydroxy acid, or a salt of such ester or amide thereof, as defined in any one of claims 1 to 9, comprising reagents necessary for measuring the level of the phospho-Akt, in a sample taken from a subject with a , and further comprising a comparator module which comprises a standard value or set of rd values of a level of the o-Akt 10 defined in claim 1 or claim 11 taken from samples of tumour tissue or circulating tumour cells of subjects with a cancer of the same histotype to which the level of the phospho-Akt in the sample is ed, wherein said reagents comprise a capture reagent comprising a detector for phospho-Akt as defined in claim 1 or claim 11 and a detector reagent, 15 and wherein the kit comprises a compound of the ing formula N N N N O or a pharmaceutically acceptable salt thereof.

26. The kit according to claim 25, wherein the capture reagent is an antibody.

27. The kit according to claim 25 or claim 26, wherein the pharmaceutically acceptable salt is the dihydrochloride salt.

28. A use as claimed in any one of claims 1 to 22 or 24, substantially as 5 herein described with reference to any e f.

29. A method as claimed in claim 23, substantially as herein described with reference to any example thereof.

30. A kit as claimed in any one of claims 25 to 27, substantially as herein described with reference to any example thereof. WO 30887