NZ585152A - Arylindenopyridines and arylindenopyrimidines and their use as adenosine A2A receptor antogonist - Google Patents

Abstract

Disclosed is a compound of Formula I or a pharmaceutically acceptable salt thereof, wherein the rest of the substituents are disclosed within the specification. Also disclosed is the use of the compound of formula I in the preparation of a pharmaceutical composition for the preparation of a medicament for treating a disorder ameliorated by antagonizing Adenosine A2a receptors such as Parkinson's Disease, Huntington's Disease and Senile Dementia. (62) Divided Out of 546212

Description

New Zealand Paient Spedficaiion for Paient Number 585152 585152 NEW ZEALAND PATENTS ACT, 1953 Divided out of NZ 546212 Date: 3 October 2003 COMPLETE SPECIFICATION We, ORTHO-MCNEIL PHARMACEUTICAL, INC., of US Route 202, Rantan, New Jersey 08869-0602, United States of America, do hereby declare the invention for which we pray that a patent may be granted to us, and the method by which it is to be performed, to be particularly described in and by the following statement: 1 585152 ARYLINDENOPYRIDINES AND ARYLINDENOPYRIMIDINES AND THEIR USE AS ADENOSINE A2A RECEPTOR ANTAGONIST Field of the Invention This invention relates to novel arylindenopyridines and 10 arylindenopyrimidines and their therapeutic and prophylactic uses. Disorders treated and/or prevented using these compounds include neurodegenerative and movement disorders ameliorated by antagonizing Adenosine A2a receptors.

Background of the Invention Adenosine A2a Receptors Adenosine is a purine nucleotide produced by all metabolically active 20 cells within the body. Adenosine exerts its effects via four subtypes of cell-surface receptors (A1, A2a, A2b and A3), which belong to the G protein coupled receptor superfamily (Stiles, G.L. Journal of Biological Chemistry, 1992, 267, 6451). A1 and A3 couple to inhibitory G protein, while A2a and A2b couple to stimulatory G protein. A2a receptors are mainly found in the 25 brain, both in neurons and glial cells (highest level in the striatum and nucleus accumbens, moderate to high level in olfactory tubercle, hypothalamus, and hippocampus etc. regions) (Rosin, D. L.; Robeva, A.; Woodard, R. L.; Guyenet, P. G.; Linden, J. Journal of Comparative Neurology ,1998, 401, 163).

In peripheral tissues, A2a receptors are found in platelets, neutrophils, vascular smooth muscle and endothelium (Gessi, S.; Varani, K.; Merighi, S.; 2 585152 Ongini, E.; Borea, P. A. British Journal of Pharmacology, 2000, 129, 2). The striatum is the main brain region for the regulation of motor activity, particularly through its innervation from dopaminergic neurons originating in the substantia nigra. The striatum is the major target of the dopaminergic 5 neuron degeneration in patients with Parkinson's Disease (PD). Within the striatum, A2a receptors are co-localized with dopamine D2 receptors, suggesting an important site for the integration of adenosine and dopamine signaling in the brain (Fink, J. S.; Weaver, D. R.; Rivkees, S. A.; Peterfreund, R. A.; Pollack, A. E.; Adler, E. M.; Reppert, S. M. Brain Research Molecular 10 Brain Research, 1992, 14, 186).

Neurochemical studies have shown that activation of A2a receptors reduces the binding affinity of D2 agonist to their receptors. This D2R and A2aR receptor-receptor interaction has been demonstrated in striatal membrane preparations of rats (Ferre, S.; von Euler, G.; Johansson, B.; 15 Fredholm, B. B.; Fuxe, K. Proceedings of the National Academy of Sciences of the United States of America, 1991, 88, 7238) as well as in fibroblast cell lines after transfected with A2aR and D2R cDNAs (Salim, H.; Ferre, S.; Dalai, A.; Peterfreund, R. A.; Fuxe, K.; Vincent, J. D.; Lledo, P. M. Journal of Neurochemistry, 2000, 74, 432). In vivo, pharmacological blockade of A2a 20 receptors using A2a antagonist leads to beneficial effects in dopaminergic neurotoxin MPTP(1-methyl-4-pheny-l,2,3,6-tetrahydropyridine)-induced PD in various species, including mice, rats, and monkeys (Ikeda, K.; Kurokawa, M.; Aoyama, S.; Kuwana, Y. Journal of Neurochemistry, 2002, 80, 262). Furthermore, A2a knockout mice with genetic blockade of A2a function have 25 been found to be less sensitive to motor impairment and neurochemical changes when they were exposed to neurotoxin MPTP (Chen, J. F.; Xu, K.; Petzer, J. P.; Staal, R.; Xu, Y. H.; Beilstein, M.; Sonsalla, P. K.; Castagnoli, K.; Castagnoli, N., Jr.; Schwarzschild, M. A. Journal of Neuroscience, 2001, 21, RC143).

In humans, the adenosine receptor antagonist theophylline has been found to produce beneficial effects in PD patients (Mally, J.; Stone, T. W. Journal of the Neurological Sciences, 1995, 132, 129). Consistently, recent epidemiological study has shown that high caffeine consumption makes people less likely to develop PD (Ascherio, A.; Zhang, S. M.; Hernan, M. A.; 3 585152 Kawachi, I.; Colditz, G. A.; Speizer, F. E.; Willett, W. C. Annals of Neurology, 2001, 50, 56). In summary, adenosine A2a receptor blockers may provide a new class of antiparkinsonian agents (Impagnatiello, F.; Bastia, E.; Ongini, E.; Monopoli, A. Emerging Therapeutic Targets, 2000, 4, 635).

Summary of the Invention This invention provides a compound having the structure of Formula I or II or a pharmaceutical^ acceptable salt thereof, wherein in Formula I: (a) R2 is selected from the group consisting of optionally substituted aryl and optionally substituted heteroaryl; optionally substituted heterocyclyl and optionally substituted C3.7 cycloalkyl, C^s alkoxy, aryloxy, C-|.8 alkylsulfonyl, arylsulfonyl, arylthio, Ci.8 alkylthio, or-NR24R25 wherein R24and R25 are independently selected from H, Ci_8 straight or branched chain alkyl, arylalkyl, C3.7 cycloalkyl, carboxyalkyl, aryl, heteroaryl, and heterocyclyl or R24and R25 taken together with the nitrogen form a heteroaryl or heterocyclyl group, (b) R3 is from one to four groups independently selected from the group consisting of: hydrogen, halo, Ci_8 straight or branched chain alkyl, arylalkyl, C3.7 cycloalkyl, Ci_8 alkoxy, cyano, C1.4 carboalkoxy, trifluoromethyl, Ci_8 alkylsulfonyl, halogen, nitro, hydroxy, Formula I Formula II 585152 trifluoromethoxy, Ci.8 carboxylate, aryl, heteroaryl, and heterocyclyl, -NR11R12, wherein R-n and R12 are independently selected from H, Ci_8 straight or branched chain alkyl, arylalkyl, C3.7 cycloalkyl, carboxyalkyl, aryl, heteroaryl, and heterocyclyl or Ri0and Rn taken together with the nitrogen form a heteroaryl or heterocyclyl group, -NR13COR14, wherein R13 is selected from hydrogen or alkyl and R14 is selected from hydrogen, alkyl, substituted alkyl, C1.3 alkoxyl, carboxyalkyl, aryl, arylalkyl, heteroaryl, heterocyclyl, Ri5RieN (CH2)P-, or Ri5Ri6NCO(CH2)p-, wherein R15 and Rie are independently selected from H, OH, alkyl, and alkoxy, and p is an integer from 1-6, wherein the alkyl group may be substituted with carboxyl, alkyl, aryl, substituted aryl, heterocyclyl, substituted heterocyclyl, heteroaryl, substituted heteroaryl, hydroxamic acid, sulfonamide, sulfonyl, hydroxy, thiol, alkoxy or arylalkyl, or R13 and R14 taken together with the carbonyl form a carbonyl containing heterocyclyl group; R4 is selected from the group consisting of hydrogen, Ci_e straight or branched chain alkyl, benzyl wherein the alkyl and benzyl groups are optionally substituted with one or more groups selected from C3.7 cycloalkyl, C1-8 alkoxy, cyano, c-m carboalkoxy, trifluoromethyl, Ci_s alkylsulfonyl, halogen, nitro, hydroxy, trifluoromethoxy, C1-8 carboxylate, amino, NR17R18, aryl and heteroaryl, -OR-I7, and —NR17R1S, wherein R17 and Ri8 are independently selected from hydrogen, and optionally substituted Ci_6 alkyl or aryl; and 585152 (d) X is selected from C=S, C=0; CH2, CHOH, CHORi9; or CHNR20R21 where Rig, R20, and R21 are selected from optionally substituted Ci_8 straight or branched chain alkyl, wherein the substituents on the alkyl group are selected from Ci_s alkoxy, hydroxy, halogen, amino, cyano, or NR22R23wherein R22and R23 are independently selected from the group consisting of hydrogen, Ci.8 straight or branched chain alkyl, C3.7 cycloalkyl, benzyl, aryl, heteroaryl, or NR22R23 taken together from a heterocycle or heteroaryl; with the proviso that said compound is not nh2 wherein Ar is phenyl, p-methoxyphenyl, m-methoxyphenyl, p- bromophenyl, m-bromophenyl, p-chlorophenyl, o-chlorophenyl, 1-naphthyl, 2-naphthyl, 2-thienyl or2-furanyl; or ir OH wherein Ar is p-chlorophenyl, 1-naphthyl, or 2-thienyl; and in Formula II: (a) R1 is selected from the group consisting of (i) -COR5, wherein R5 is selected from H, optionally substituted C-i_8 straight or branched chain alkyl, optionally substituted aryl and optionally substituted arylalkyl; wherein the substituents on the alkyl, aryl and arylalkyl group are selected from C-i-s alkoxy, phenylacetyloxy, hydroxy, halogen, p-tosyloxy, mesyloxy, amino, cyano, carboalkoxy, or NR7R8 wherein R7 and Rs are Ar 585152 independently selected from the group consisting of hydrogen, Ci_s straight or branched chain alkyl, C3.7 cycloalkyl, benzyl, aryl, or heteroaryl or NR7R8 taken together form a heterocycle or heteroaryl; (ii) COOR5, wherein R5 is as defined above; (ii) cyano; (iii) -CONR9R10 wherein R9and R10 are independently selected from H, Ci_s straight or branched chain alkyl, C3. 7 cycloalkyl, trifluoromethyl, hydroxy, alkoxy, acyl, alkylcarbonyl, carboxyl, arylalkyl, aryl, heteroaryl and heterocyclyl; wherein the alkyl, cycloalkyl, alkoxy, acyl, alkylcarbonyl, carboxyl, arylalkyl, aryl, heteroaryl and heterocyclyl groups may be substituted with carboxyl, alkyl, aryl, substituted aryl, heterocyclyl, substituted heterocyclyl, heteroaryl, substituted heteroaryl, hydroxamic acid, sulfonamide, sulfonyl, hydroxy, thiol, amino, alkoxy or arylalkyl, or R9 and R10 taken together with the nitrogen to which they are attached form a heterocycle or heteroaryl group; (v) optionally substituted Ci_8 straight or branched chain alkyl; wherein the substituents on the alkyl, group are selected from C-i-8 alkoxy, phenylacetyloxy, hydroxy, halogen, p-tosyloxy, mesyloxy, amino, cyano, carboalkoxy, carboxyl, aryl, heterocyclyl, heteroaryl, sulfonyl, thiol, alkylthio, or NR7R8 wherein R7 and Rs are as defined above; R2 is selected from the group consisting of optionally substituted alkyl, optionally substituted aryl, optionally substituted heteroaryl, optionally substituted heterocyclyl and optionally substituted C3.7 cycloalkyl, Ci_8 alkoxy, aryloxy, Ci_8 alkylsulfonyl, arylsulfonyl, arylthio, Ci_s alkylthio, or-NR24R25 wherein R24and R25 are independently selected from H, Ci_s straight or branched chain alkyl, arylalkyl, C3.7 585152 cycloalkyl, carboxyalkyl, aryl, heteroaryl, and heterocyclyl or f^and R25 taken together with the nitrogen form a heteroaryl or heterocyclyl group, R3 is from one to four groups independently selected from the group consisting of: hydrogen, halo, Ci.8 straight or branched chain alkyl, arylalkyl, C3.7 cycloalkyl, C1-8 alkoxy, cyano, C-m carboalkoxy, trifluoromethyl, Ci_s alkylsulfonyl, halogen, nitro, hydroxy, trifluoromethoxy, Ci_s carboxylate, aryl, heteroaryl, and heterocyclyl, -NR11R12, wherein R-n and R-12 are independently selected from H, C*i_8 straight or branched chain alkyl, arylalkyl, C3.7 cycloalkyl, carboxyalkyl, aryl, heteroaryl, and heterocyclyl or Rioand Rn taken together with the nitrogen form a heteroaryl or heterocyclyl group, -NR13COR14, wherein R13 is selected from hydrogen or alkyl and R14 is selected from hydrogen, alkyl, substituted alkyl, C1.3 alkoxyl, carboxyalkyl, aryl, arylalkyl, heteroaryl, heterocyclyl, R15RieN (CH2)P-, or R15Ri6NCO(CH2)p-, wherein R15 and Ri6 are independently selected from H, OH, alkyl, and alkoxy, and p is an integer from 1-6, wherein the alkyl group may be substituted with carboxyl, alkyl, aryl, substituted aryl, heterocyclyl, substituted heterocyclyl, heteroaryl, substituted heteroaryl, hydroxamic acid, sulfonamide, sulfonyl, hydroxy, thiol, alkoxy or arylalkyl, or Ri3 and R14 taken together with the carbonyl form a carbonyl containing heterocyclyl group; and X is selected from C=S, C=0; CH2, CHOH, CHOR19; or CHNR20R21 where R19, R2o, and R2i are selected from optionally substituted Ci_8 straight of branched chain alkyl, wherein the substituents on the alkyl group are selected from Ci_8 alkoxy, 8 585152 hydroxy, halogen, amino, cyano, or NR22R23wherein R22and R23 are independently selected from the group consisting of hydrogen, Ci_8 straight or branched chain alkyl, C3.7 cycloalkyl, benzyl, aryl, heteroaryl, or NR22R23 taken together from a 5 heterocycle or heteroaryl; with the proviso that when R1 is a cyano, then R2 is not phenyl.

This invention also provides a pharmaceutical composition comprising the instant compound and a pharmaceutical^ acceptable carrier. In this embodiment, the proviso relating to the compounds of Formula I does not 10 apply.

In another embodiment, the invention relates to the use of a compound of Formula I or Formula II, or a pharmaceutical composition of the invention, for the preparation of a medicament for treating a subject having a disorder ameliorated by antagonizing Adenosine A2a receptors in appropriate 15 cells in the subject, wherein the disorder is a neurodegenerative disorder or a movement disorder, wherein the proviso relating to the compounds of Formula I does not apply.

Also described herein is a method of treating a subject having a condition ameliorated by antagonizing Adenosine A2a receptors, which 20 comprises administering to the subject a therapeutically effective dose of the instant pharmaceutical composition.

Also described herein is a method of preventing a disorder ameliorated by antagonizing Adenosine A2a receptors in a subject, comprising of administering to the subject a prophylactically effective dose of the compound 25 of Formula I or Formula II either preceding or subsequent to an event anticipated to cause a disorder ameliorated by antagonizing Adenosine A2a receptors in the subject.

Detailed Description of the Invention Compounds of Formula I are potent small molecule antagonists of the Adenosine A2a receptors that have demonstrated potency for the antagonism of Adenosine A2a, A1, and A3 receptors. 9 585152 Preferred embodiments for Ri are COOR5 wherein R5 is an optionally substituted Ci_s straight or branched chain alkyl. Preferably the alkyl chain is substituted with a dialkylamino group.

Preferred embodiments for R2 in Formula II are optionally substituted 5 heteroaryl and optionally substituted aryl. Preferably, in Formula I and Formula II R2 is an optionally substituted furan.

Preferred substituents for R3 include hydrogen, halo, hydroxy, amino, trifluoromethyl, alkoxy, hydroxyalkyl chains, and aminoalkyl chains.

Preferred substituents for R4 include NH2 and alkylamino.

In a preferred embodiment, the compound is selected from the group of compounds shown in Tables 1 and 2 hereinafter.

More preferably, the compound is selected from the following compounds: The compound of claim 1, formula I, wherein R4 is amino. 2-amino-4-furan-2-yl-indeno[1,2-d]pyrimidin-5-one 2-amino-4-phenyl-indeno[1,2-d]pyrimidin-5-one 585152 2-amino-4-thiophen-2-yl-indeno[1,2-d]pyrimidin-5-one 2-amino-4-(5-methyl-furan-2-yl)-indeno[1,2-d]pyrimidin-5-one 2,6-diamino-4-furan-2-yl-indeno[1,2-d]pyrimidin-5-one 9/-/-indeno[2,1-c]pyridine-4-carbonitrile, 3-amino-1-furan-2-yl-9-oxo- 11 585152 9/-/-indeno[2,1-c]pyridine-4-carboxylic acid, 3-amino-1-furan-2-yl-9-oxo-, 2- dimethylamino-ethyl ester 9/-/-indeno[2,1-c]pyridine-4-carboxylic acid, 3-amino-1-phenyl-9-oxo-, 2- dimethylamino-ethyl ester 9H-indeno[2,1-c]pyridine-4-carboxylic acid, 3-amino1-furan-2-yl-9-oxo-, (2-dimethylamino-1-methyl-ethyl)-amide 12 585152 9/-/-indeno[2,1-c]pyridine-4-carboxylic acid, 3-amino-1-furan-2-yl-9-oxo-, (2-dimethylamino-ethyl)-methyl-amide 9H-indeno[2,1-c]pyridine-4-carboxylic acid, 3-amino-1-furan-2-yl-9-oxo-, 1-5 methyl-pyrrolidin-2-ylmethyl ester The instant compounds can be isolated and used as free bases. They can also be isolated and used as pharmaceutically acceptable salts. Examples of such salts include hydrobromic, hydroiodic, hydrochloric, 10 perchloric, sulfuric, maleic, fumaric, malic, tartaric, citric, benzoic, mandelic, methanesulfonic, hydroethanesulfonic, benzenesulfonic, oxalic, palmoic, 2-naphthalenesulfonic, p-toluenesulfonic, cyclohexanesulfamic and saccharic.

This invention also provides a pharmaceutical composition comprising the instant compound and a pharmaceutically acceptable carrier. In this 15 embodiment, the proviso relating to the compounds of Formula I does not apply.

Pharmaceutically acceptable carriers are well known to those skilled in the art and include, but are not limited to, from about 0.01 to about 0.1 M and preferably 0.05 M phosphate buffer or 0.8% saline. Such pharmaceutically acceptable carriers can be aqueous or non-aqueous solutions, suspensions and emulsions. Examples of non-aqueous solvents are propylene glycol, polyethylene glycol, vegetable oils such as olive oil, and injectable organic esters such as ethyl oleate. Aqueous carriers include water, ethanol, alcoholic/aqueous solutions, glycerol, emulsions or suspensions, including saline and buffered media. Oral carriers can be elixirs, syrups, capsules, tablets and the like. The typical solid carrier is an inert substance such as lactose, starch, glucose, methyl-cellulose, magnesium stearate, dicalcium phosphate, mannitol and the like. Parenteral carriers include sodium chloride 13 585152 solution, Ringer's dextrose, dextrose and sodium chloride, lactated Ringer's and fixed oils. Intravenous carriers include fluid and nutrient replenishers, electrolyte replenishers such as those based on Ringer's dextrose and the like. Preservatives and other additives can also be present, such as, for example, 5 antimicrobials, antioxidants, chelating agents, inert gases and the like. All carriers can be mixed as needed with disintegrants, diluents, granulating agents, lubricants, binders and the like using conventional techniques known in the art.

Also described herein is a method of treating a subject having a 10 condition ameliorated by antagonizing Adenosine A2a receptors, which comprises administering to the subject a therapeutically effective dose of the instant pharmaceutical composition.

In one embodiment, the disorder is a neurodegenerative or movement disorder. Examples of disorders treatable by the instant pharmaceutical 15 composition include, without limitation, Parkinson's Disease, Huntington's Disease, Multiple System Atrophy, Corticobasal Degeneration, Alzheimer's Disease, and Senile Dementia.

In one preferred embodiment, the disorder is Parkinson's disease.

As used herein, the term "subject" includes, without limitation, any 20 animal or artificially modified animal having a disorder ameliorated by antagonizing adenosine A2a receptors. In a preferred embodiment, the subject is a human.

Administering the instant pharmaceutical composition can be effected or performed using any of the various methods known to those skilled in the 25 art. The instant compounds can be administered, for example, intravenously, intramuscularly, orally and subcutaneously. In the preferred embodiment, the instant pharmaceutical composition is administered orally. Additionally, administration can comprise giving the subject a plurality of dosages over a suitable period of time. Such administration regimens can be determined 30 according to routine methods.

As used herein, a "therapeutically effective dose" of a pharmaceutical composition is an amount sufficient to stop, reverse or reduce the progression of a disorder. A "prophylactically effective dose" of a pharmaceutical composition is an amount sufficient to prevent a disorder, i.e., 14 585152 eliminate, ameliorate and/or delay the disorder's onset. Methods are known in the art for determining therapeutically and prophylactically effective doses for the instant pharmaceutical composition. The effective dose for administering the pharmaceutical composition to a human, for example, can 5 be determined mathematically from the results of animal studies.

In one embodiment, the therapeutically and/or prophylactically effective dose is a dose sufficient to deliver from about 0.001 mg/kg of body weight to about 200 mg/kg of body weight of the instant pharmaceutical composition. In another embodiment, the therapeutically and/or 10 prophylactically effective dose is a dose sufficient to deliver from about 0.05 mg/kg of body weight to about 50 mg/kg of body weight. More specifically, in one embodiment, oral doses range from about 0.05 mg/kg to about 100 mg/kg daily. In another embodiment, oral doses range from about 0.05 mg/kg to about 50 mg/kg daily, and in a further embodiment, from about 0.05 15 mg/kg to about 20 mg/kg daily. In yet another embodiment, infusion doses range from about 1.0 |j,g/kg/min to about 10 mg/kg/min of inhibitor, admixed with a pharmaceutical carrier over a period ranging from about several minutes to about several days. In a further embodiment, for topical administration, the instant compound can be combined with a pharmaceutical 20 carrier at a drug/carrier ratio of from about 0.001 to about 0.1.

Definitions and Nomenclature Unless otherwise noted, under standard nomenclature used throughout 25 this disclosure the terminal portion of the designated side chain is described first, followed by the adjacent functionality toward the point of attachment.

As used herein, the following chemical terms shall have the meanings as set forth in the following paragraphs: "independently", when in reference to chemical substituents, shall mean that when more than one substituent 30 exists, the substituents may be the same or different;.

"Alkyl" shall mean straight, cyclic and branched-chain alkyl. Unless otherwise stated, the alkyl group will contain 1-20 carbon atoms. Unless otherwise stated, the alkyl group may be optionally substituted with one or more groups such as halogen, OH, CN, mercapto, nitro, amino, Ci-Cs-alkyl, 585152 CrC8-alkoxyl, CrC8-alkylthio, CrC8-alkyl-amino, di(CrC8-alkyl)amino, (mono-, di-, tri-, and per-) halo-alkyl, formyl, carboxy, alkoxycarbonyl, Ci-C8-alkyl-CO-O-, CrCs-alkyl-CO-NH-, carboxamide, hydroxamic acid, sulfonamide, sulfonyl, thiol, aryl, aryl(ci-c8)alkyl, heterocyclyl, and heteroaryl.

"Alkoxy" shall mean -O-alkyl and unless otherwise stated, it will have 1-8 carbon atoms.

The term "bioisostere" is defined as "groups or molecules which have chemical and physical properties producing broadly similar biological properties." (Burger's Medicinal Chemistry and Drug Discovery, M. E. Wolff, 10 ed. Fifth Edition, Vol. 1, 1995, Pg. 785).

"Halogen" shall mean fluorine, chlorine, bromine or iodine; "PH" or "Ph" shall mean phenyl; "Ac" shall mean acyl; "Bn" shall mean benzyl.

The term "acyl" as used herein, whether used alone or as part of a substituent group, means an organic radical having 2 to 6 carbon atoms 15 (branched or straight chain) derived from an organic acid by removal of the hydroxyl group. The term "Ac" as used herein, whether used alone or as part of a substituent group, means acetyl.

"Aryl" or "Ar," whether used alone or as part of a substituent group, is a carbocyclic aromatic radical including, but not limited to, phenyl, 1- or2-20 naphthyl and the like. The carbocyclic aromatic radical may be substituted by independent replacement of 1 to 5 of the hydrogen atoms thereon with halogen, OH, CN, mercapto, nitro, amino, CrC8-alkyl, CrC8-alkoxyl, CrC8-alkylthio, CrC8-alkyl-amino, di(CrC8-alkyl)amino, (mono-, di-, tri-, and per-) halo-alkyl, formyl, carboxy, alkoxycarbonyl, CrC8-alkyl-C0-0-, CrC8-alkyl-25 CO-NH-, or carboxamide. Illustrative aryl radicals include, for example, phenyl, naphthyl, biphenyl, fluorophenyl, difluorophenyl, benzyl, benzoyloxyphenyl, carboethoxyphenyl, acetylphenyl, ethoxyphenyl, phenoxyphenyl, hydroxyphenyl, carboxyphenyl, trifluoromethylphenyl, methoxyethylphenyl, acetamidophenyl, tolyl, xylyl, dimethylcarbamylphenyl 30 and the like. "Ph" or "PH" denotes phenyl.

Whether used alone or as part of a substituent group, "heteroaryl" refers to a cyclic, fully unsaturated radical having from five to ten ring atoms of which one ring atom is selected from S, O, and N; 0-2 ring atoms are additional heteroatoms independently selected from S, O, and N; and the 16 585152 remaining ring atoms are carbon. The radical may be joined to the rest of the molecule via any of the ring atoms. Exemplary heteroaryl groups include, for example, pyridinyl, pyrazinyl, pyrimidinyl, pyridazinyl, pyrroyl, pyrazolyl, imidazolyl, thiazolyl, oxazolyl, isoxazolyl, thiadiazolyl, triazolyl, triazinyl, 5 oxadiazolyl, thienyl, furanyl, quinolinyl, isoquinolinyl, indolyl, isothiazolyl, 2-oxazepinyl, azepinyl, N-oxo-pyridyl, 1-dioxothienyl, benzothiazolyl, benzoxazolyl, benzothienyl, quinolinyl-N-oxide, benzimidazolyl, benzopyranyl, benzisothiazolyl, benzisoxazolyl, benzodiazinyl, benzofurazanyl, benzothiopyranyl, indazolyl, indolizinyl, benzofuryl, chromonyl, coumarinyl, 10 cinnolinyl, quinoxalinyl, indazolyl, pyrrolopyridinyl, furopyridinyl (such as furo[2,3-c]pyridinyl, furo[3,2-b]pyridinyl, or furo[2,3-b]pyridinyl), imidazopyridinyl (such as imidazo[4,5-b]pyridinyl or imidazo[4,5-c]pyridinyl), naphthyridinyl, phthalazinyl, purinyl, pyridopyridyl, quinazolinyl, thienofuryl, thienopyridyl, thienothienyl, and furyl. The heteroaryl group may be 15 substituted by independent replacement of 1 to 5 of the hydrogen atoms thereon with halogen, OH, CN, mercapto, nitro, amino, CrC8-alkyl, CrC8-alkoxyl, CrC8-alkylthio, CrC8-alkyl-amino, di(CrC8-alkyl)amino, (mono-, di-, tri-, and per-) halo-alkyl, formyl, carboxy, alkoxycarbonyl, CrC8-alkyl-C0-0-, Ci-C8-alkyl-CO-NH-, or carboxamide. Heteroaryl may be substituted with a 20 mono-oxo to give for example a 4-oxo-1 H-quinoline.

The terms "heterocycle," "heterocyclic," and "heterocyclo" refer to an optionally substituted, fully or partially saturated cyclic group which is, for example, a 4- to 7-membered monocyclic, 7- to 11-membered bicyclic, or 10-to 15-membered tricyclic ring system, which has at least one heteroatom in at 25 least one carbon atom containing ring. Each ring of the heterocyclic group containing a heteroatom may have 1, 2, or 3 heteroatoms selected from nitrogen atoms, oxygen atoms, and sulfur atoms, where the nitrogen and sulfur heteroatoms may also optionally be oxidized. The nitrogen atoms may optionally be quaternized. The heterocyclic group may be attached at any 30 heteroatom or carbon atom.

Exemplary monocyclic heterocyclic groups include pyrrolidinyl; oxetanyl; pyrazolinyl; imidazolinyl; imidazolidinyl; oxazolyl; oxazolidinyl; isoxazolinyl; thiazolidinyl; isothiazolidinyl; tetrahydrofuryl; piperidinyl; piperazinyl; 2-oxopiperazinyl; 2-oxopiperidinyl; 2-oxopyrrolidinyl; 4- 17 585152 piperidonyl; tetrahydropyranyl; tetrahydrothiopyranyl; tetrahydrothiopyranyl sulfone; morpholinyl; thiomorpholinyl; thiomorpholinyl sulfoxide; thiomorpholinyl sulfone; 1,3-dioxolane; dioxanyl; thietanyl; thiiranyl; and the like. Exemplary bicyclic heterocyclic groups include quinuclidinyl; tetrahydroisoquinolinyl; dihydroisoindolyl; dihydroquinazolinyl (such as 3,4-dihydro-4-oxo-quinazolinyl); dihydrobenzofuryl; dihydrobenzothienyl; dihydrobenzothiopyranyl; dihydrobenzothiopyranyl sulfone; dihydrobenzopyranyl; indolinyl; isochromanyl; isoindolinyl; piperonyl; tetrahydroquinolinyl; and the like.

Substituted aryl, substituted heteroaryl, and substituted heterocycle may also be substituted with a second substituted-aryl, a second substituted-heteroaryl, or a second substituted-heterocycle to give, for example, a 4-pyrazol-1-yl-phenyl or 4-pyridin-2-yl-phenyl.

Designated numbers of carbon atoms (e.g., Ci_s) shall refer 15 independently to the number of carbon atoms in an alkyl or cycloalkyl moiety or to the alkyl portion of a larger substituent in which alkyl appears as its prefix root.

Unless specified otherwise, it is intended that the definition of any substituent or variable at a particular location in a molecule be independent of 20 its definitions elsewhere in that molecule. It is understood that substituents and substitution patterns on the compounds of this invention can be selected by one of ordinary skill in the art to provide compounds that are chemically stable and that can be readily synthesized by techniques known in the art as well as those methods set forth herein.

Where the compounds according to this invention have at least one stereogenic center, they may accordingly exist as enantiomers. Where the compounds possess two or more stereogenic centers, they may additionally exist as diastereomers. Furthermore, some of the crystalline forms for the compounds may exist as polymorphs and as such are intended to be 30 included in the present invention. In addition, some of the compounds may form solvates with water (i.e., hydrates) or common organic solvents, and such solvates are also intended to be encompassed within the scope of this invention. 18 585152 Some of the compounds of the present invention may have trans and cis isomers. In addition, where the processes for the preparation of the compounds according to the invention give rise to mixture of stereoisomers, these isomers may be separated by conventional techniques such as 5 preparative chromatography. The compounds may be prepared as a single stereoisomer or in racemic form as a mixture of some possible stereoisomers. The non-racemic forms may be obtained by either synthesis or resolution. The compounds may, for example, be resolved into their components enantiomers by standard techniques, such as the formation of 10 diastereomeric pairs by salt formation. The compounds may also be resolved by covalent linkage to a chiral auxiliary, followed by chromatographic separation and/or crystallographic separation, and removal of the chiral auxiliary. Alternatively, the compounds may be resolved using chiral chromatography.

This invention will be better understood by reference to the Experimental Details which follow, but those skilled in the art will readily appreciate that these are only illustrative of the invention as described more fully in the claims which follow thereafter. Additionally, throughout this application, various publications are cited. The disclosure of these publications is hereby incorporated by 20 reference into this application to describe more fully the state of the art to which this invention pertains.

Experimental Details I. General Synthetic Schemes Representative compounds of the present invention can be synthesized in accordance with the general synthetic methods described below and illustrated in the following general schemes. The products of some schemes can be used as intermediates to produce more than one of 30 the instant compounds. The choice of intermediates to be used to produce subsequent compounds of the present invention is a matter of discretion that is well within the capabilities of those skilled in the art. 19 585152 Procedures described in Schemes 1 to 7, wherein R3a, R3b, R30 and R3d are independently any R3 group, and R-i, R2, R3, and R4 are as described above, can be used to prepare compounds of the invention.

The substituted pyrimidines 1 can be prepared as shown in Scheme 1.

The indanone or indandione 2 or the indene ester 3 can be condensed with an aldehyde to yield the substituted benzylidenes 4 (Bullington, J.L; Cameron, J.C.; Davis, J.E.; Dodd, J.H.; Harris, C.A.; Henry, J.R.; Pellegrino-Gensey, J.L.; Rupert, K.C.; Siekierka, J.J. Bioorg. Med. Chem. Lett. 1998, 8, 2489; Petrow, V.; Saper, J.; Sturgeon, B. J. Chem. Soc. 1949, 2134). . This 10 is then condensed with guanidine carbonate to form the indenopyrimidine 1.

Scheme 1 Alternatively, the pyrimidine compounds can be prepared as shown in Scheme 2. Sulfone 6 can be prepared by oxidation of the thiol ether 5 and 15 the desired amines 7 can be obtained by treatment of the sulfone with aromatic amines. 585152 Scheme 2 cs2 o KF CH3I DMF O O O N SCH3 Oxone MeOH h2o O so2ch3 hnr'2 N=( N nh2 6 N=( NH2 o nr'2 Pyrimidines with substituents on the fused aromatic ring could also be synthesized by the following procedure (Scheme 3). The synthesis starts 5 with alkylation of furan with allyl bromide to provide 2-allylfuran. Diels-Alder reaction of 2-allylfuran with dimethylacetylene dicarboxylate followed by deoxygenation (Xing, Y.D.; Huang, N.Z. J. Org. Chem. 1982, 47, 140) provided the phthalate ester 8. The phthalate ester 8 then undergoes a Claisen condensation with ethyl acetate to give the styryl indanedione 9 after 10 acidic workup (Buckle, D.R.; Morgan, N.J.; Ross, J.W.; Smith, H.; Spicer, B.A. J. Med. Chem. 1973, 16, 1334). The indanedione 9 is then converted to the dimethylketene dithioacetal 10 using carbon disulfide in the presence of kf. Addition of Grignard reagents to the dithioacetal 10 and subsequent reaction with guanidine provides the pyrimidines 11 as a mixture of isomers. 21 585152 Scheme 3 R3a> R3d = CHCHCH3, H Dihydroxylation and oxidation give the aromatic aldehydes 13 that can be reductively aminated to provide amines 14. The other isomer can be treated in a similar manner.

Scheme 4 OH NH, NHc 13 14 NaIOz THF 3-Dicyanovinylindan-1-one (15) (Scheme 5) was obtained using the published procedure (Bello, K.A.; Cheng, L.; Griffiths, J. J. Chem. Soc., 10 PerkinTrans.il 1987,815). Reaction of 3-dicyanovinylindan-1-one with an aldehyde in the presence of ammonium hydroxide produced dihydropyridines 16 (El-Taweel, F.M.A.; Sofan, M.A.; E.-Maati, T.M.A.; Elagamey, A.A. Boll. Chim. Farmac. 2001, 140, 306). These compounds were then oxidized to the corresponding pyridines 17 using chromium trioxide in refluxing acetic acid. 22 585152 Scheme 5 The ketone of pyridines 17 can be reduced to provide the benzylic alcohols 18. Alternatively, the nitriles can be hydrolyzed with sodium 5 hydroxide to give the carboxylic acids 19 (Scheme 6).

Scheme 6 The acids can then be converted to carboxylic esters 20 or amides 21 using a variety of methods. In general, the esters 20 are obtained by treatment with silver carbonate followed by an alkyl chloride or by coupling 10 with diethylphosphoryl cyanide (DEPC) and the appropriate alcohol (Okawa, T.; Toda, M.; Eguchi, S.; Kakehi, A. Synthesis 1998, 1467). The amides 21 are obtained by coupling the carboxylic acid with the appropriate amine in the presence of DEPC or 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDCI). Esters 20 can also be obtained by first reacting the 23 585152 carboxylic acids 19 with a dibromoalkane followed by displacement of the terminal bromide with an amine (Scheme 7).

Scheme 7 II. Specific Compound Syntheses Specific compounds which are representative of this invention can be prepared as per the following examples. No attempt has been made to optimize the yields obtained in these reactions. Based on the following, 10 however, one skilled in the art would know how to increase yields through routine variations in reaction times, temperatures, solvents and/or reagents.

The products of certain syntheses can be used as intermediates to produce more than one of the instant compounds. In those cases, the choice of intermediates to be used to produce compounds of the present invention is 15 a matter of discretion that is well within the capabilities of those skilled in the art.

A mixture of 3 (3.0 g, 11.69 mmol) and 2-furaldehyde (1.17 g, 12.17 mmol) in 75 ml_ of ethanol and 3 ml. of concentrated hydrogen chloride was allowed to stir at reflux for 16 hours. The reaction was then cooled to room 25 temperature, and the resulting precipitate was filtered off, washed with ethanol, diethyl ether, and air dried to afford 1.27 g (45%) of product. 19 R-| = C02R 21 R-| = CONR'2 EXAMPLE 1 Synthesis of Benzvlidene 4 (R? = 2-furyl, Rga = F, Rgh, R^.Rgd = H) 24 585152 EXAMPLE 2 Synthesis of Indenopvrimidine 1 (R? = 2-furvl, R3a = F. R^h. R^.R^h = H) A mixture of 4 (0.5 g, 2.06 mmol), guanidine carbonate (0.93 g, 5.16 mmol), and 20.6 mL of 0.5 M sodium methoxide in methanol was stirred at reflux for 16 hours. The reaction mixture was cooled to room temperature, and diluted with water. The resulting precipitate was collected, washed with water, ethanol, diethyl ether, and then dried. Crude material was then 10 purified over silica gel to afford 0.024 g (4%) of product. MS m/z 282.0 (M+H).

EXAMPLE 3 Synthesis of 2-Amino-4-methanesulfonyl-indenori ,2-dlpyrimidin-5-one To a suspension of 5 (Augustin, M.; Groth, C.; Kristen, H.; Peseke, K.; Wiechmann, C. J. Prakt. Chem. 1979, 321, 205) (1.97 g, 8.10 mmol) in MeOH (150 mL) was added a solution of oxone (14.94 g, 24.3 mmol) in H20 (100 mL). The mixture was stirred at room temperature overnight then 20 diluted with cold H20 (500 mL), made basic with K2C03 and filtered. The product was washed with water and ether to give 0.88 g (40%) of sulfone 6. MS m/z 297.9 (M+Na).

EXAMPLE 4 Synthesis of Aminopyrimidine 7 (R? = NHPh, R* = H) A mixture of sulfone 6 (0.20 g, 0.73 mmol) and aniline (0.20 g, 2.19 mmol) in N-methylpyrrolidinone (3.5 mL) was heated to 100 °C for 90 30 minutes. After cooling to room temperature, the mixture was diluted with EtOAc (100 mL), washed with brine (2 x 75 mL) and water (2 x 75 mL), and dried over Na2SC>4. After filtration and concentration in vacuo, the residue was purified by column chromatography eluting with 0-50% EtOAc in hexane to yield 0.0883 g (42%) of product 7. MS m/z 289.0 (M+H). 585152 EXAMPLE 5 Synthesis of Phthalate Ester 8 A 1.37 M hexanes solution of n-BuLi (53.6 mL, 73.4 mmol) was added to a cold, -78°C, THF solution (100 mL) of furan (5.3 mL, 73.4 mmol) and the reaction was then warmed to 0 °C. After 1.25 h at 0°C neat allyl bromide (7.9 mL, 91.8 mmol) was added in one portion. After 1 h at 0°C, saturated aqueous NH4CI was added and the layers were separated. The aqueous 10 phase was extracted with EtOAc and the combined organics were washed with water and brine, dried over Na2S04, and concentrated to give 4.6 g (58%) of 2-allylfuran which was used without further purification.

The crude allyl furan (4.6 g, 42.6 mmol) and dimethylacetylene dicarboxylate (5.2 mL, 42.6 mmol) were heated to 90°C in a sealed tube 15 without solvent. After 6 h at 90°C the material was cooled and purified by column chromatography eluting with 25% EtOAc in hexanes to give 5.8 g (54%) of the oxabicycle as a yellow oil. MS m/z 251 (M+H).

Tetrahydrofuran (60 mL) was added dropwise to neat TiCU (16.5 mL, 150.8 mmol) at 0°C. A 1.0 M THF solution of LiAIH4 (60.3 mL, 60.3 mmol) 20 was added dropwise, changing the color of the suspension from yellow to a dark green or black suspension. Triethylamine (2.9 mL, 20.9 mmol) was added and the mixture was refluxed at 75-80°C. After 45 min, the solution was cooled to rt and a THF solution (23 mL) of the oxabicycle (5.8 g, 23.2 mmol) was added to the dark solution. After 2.5 h at rt, the solution was 25 poured into a 20% aq. K2CO3 solution (200 mL) and the resulting suspension was filtered. The precipitate was washed several times with CH2CI2 and the filtrate layers were separated. The aqueous phase was extracted with CH2CI2 and the combined organics were washed with water and brine, dried over Na2S04, concentrated, and purified by column chromatography eluting with 30 25 % EtOAc in hexanes to give 3.5 g (64%) of the phthalate ester 8 as a yellow oil. MS m/z 235 (M+H). 26 585152 EXAMPLE 6 Synthesis of Indanedione 9 A 60% dispersion of sodium hydride in mineral oil (641 mg, 16.0 mmol) 5 was added to an EtOAc solution (3.5 mL) of the phthalate ester 8 (2.5 g, 10.7 mmol), and the resulting slurry was refluxed. After 1 h the solution became viscous so an additional 7.5 mL of EtOAc was added. After 4 h at reflux the suspension was cooled to rt and filtered to give a yellow solid. This solid was added portionwise to a solution of HCI (25 mL water and 5 mL conc. HCI) at 10 80°C. The suspension was heated for an additional 30 min at 80°C, cooled to rt, and filtered to give 1.2 g (60%) of the indanedione 9 as a yellow solid. MS m/z 187 (M+H).

EXAMPLE 7 Synthesis of Dimethylketene Dithioacetal 10 Solid potassium fluoride (7.5 g, 129.1 mmol) was added to a 0°C solution of indanedione 9 (1.2 g, 6.5 mmol) and CS2 (0.47 mL, 7.8 mmol) in DMF (10 mL). The cold bath was removed and after 30 min neat 20 iodomethane (1.00 mL, 16.3 mmol) was added. After 5 h at rt, the suspension was diluted with EtOAc and then washed with water and brine. The organic layer was dried over Na2S04, concentrated, and purified by column chromatography eluting with 20 % EtOAc in hexanes to give 1.4 g (75%) of the dimethylketene dithioacetal 10 as a yellow solid. MS m/z 291 25 (M+H).

EXAMPLE 8 Synthesis of Pyrimidine 11 (R?=Ph, R^=CHCHCH3. R^=H) A 2.0 M solution of PhMgCI in THF (13 mL, 25.7 mmol) was added to a -78°C solution of dimethylketene dithioacetal 10 (5.7 g, 19.8 mmol) in 200 mL of THF. After 3 h at -78°C, saturated aqueous NH4CI was added and the 27 585152 layers were separated. The aqueous layer was extracted with EtOAc and the combined organic extracts were washed with water and brine, dried over Na2S04, concentrated, and purified by column chromatography eluting with 20 % EtOAc in hexanes to give 4.9 g (77%) of the thioenol ether as a yellow 5 solid. MS m/z 321 (M+H).

Solid guanidine hydrochloride (1.5 g, 15.3 mmol) was added to a solution of the thioenol ether (4.9 g, 15.3 mmol) and K2C03 (2.6 g, 19.1 mmol) in 30 mL of DMF and the solution was heated to 80°C. After 6 h at 80°C, the solution was diluted with EtOAc and washed with water and brine. 10 The organic layer was dried over Na2S04, concentrated, and purified by column chromatography eluting with 40 % EtOAc in hexanes to give 4.6 g (96%) of the pyrimidine regioisomers 11 as yellow solids. MS m/z 314 (M+H).

EXAMPLE 9 Synthesis of Aldehyde 13 (R?=Ph) Solid MeS02NH2 (277 mg, 2.9 mmol) was added to a t-Bu0H:H20 (1:1) solution (30 mL) of AD-mix-a (4.0 g). The resulting yellow solution was 20 added to an EtOAc solution (15 mL) of the pyrimidine (910 mg, 2.9 mmol). After 3 days, solid sodium sulfite (4.4 g, 34.9 mmol) was added. After stirring for 1.5 h, the heterogeneous solution was diluted with EtOAc and the layers were separated. The aqueous phase was extracted with EtOAc and the combined extracts were washed with water and brine, dried over Na2S04, 25 concentrated, and purified by column chromatography eluting with 100 % EtOAc to give 710 mg (70%) of the intermediate diol 12. MS m/z 348 (M+H).

Solid HI04-2H20 (933 mg, 4.1 mmol) was added to a 0 °C solution of diol 12 (710 mg, 2.1 mmol) in THF. After 1.5 h at 0°C, the solution was diluted with EtOAc and the organic phase was washed with saturated 30 aqueous NaHCOs, water, and brine. The organic layer was dried over Na2S04 and concentrated to give 603 mg (98%) of aldehyde 13 as a yellow solid that was used without further purification. MS m/z 302 (M+H). 28 585152 EXAMPLE 10 Synthesis of Amine 14 via Reductive Amination (R^= N(-CH?CH?OCH?CH?-) Solid NaBH(OAc)3 (53 mg, 0.25 mmol) was added to a solution of aldehyde 13 (50 mg, 0.17 mmol), morpholine (0.034 mL, 0.34 mmol), and AcOH (0.014 mL, 0.25 mmol) in 1 mL of THF. After 3 d the solution was filtered and concentrated. The resulting material was dissolved in CH2CI2 and washed with saturated aqueous NaHCC>3 and brine, dried over Na2SC>4, concentrated, and purified by column chromatography eluting with 0-10 % MeOH in CH2CI2 to give 38 mg (60%) of the amine 14 as a yellow solid. MS m/z 373 (M+H). The product was dissolved in a minimum amount of CH2CI2 and treated with 1.0 M HCI in ether to obtain the hydrochloride salt.

EXAMPLE 11 Cvclization to Form Dihydropyridine 16 (R? = 2-furvl, R^=H) To a solution of 3-dicyanovinylindan-1-one (4.06 g, 20.9 mmol) in 200 mL of ethanol was added 2-furaldehyde (3.01 g, 31.4 mmol) and 25 mL of conc. NH4OH. The solution was heated to reflux for 2 h and allowed to cool to rt overnight. The mixture was concentrated in vacuo to remove ethanol. The residue was filtered and washed with water. The purple solid obtained was dried to yield 5.92 g (89%). MS m/z 290 (M++1).

EXAMPLE 12 Oxidation of Dihydropyridine 16 to Pyridine 17 (R? = 2-furvl. R^ = H. Ra = NH?, R^ = CN. X = O) To a refluxing solution of dihydropyridine 16 (5.92 g, 20.4 mmol) in acetic acid (100 mL) was added a solution of chromium (VI) oxide (2.05 g, 20.4 mmol) in 12 mL of water. After 10 minutes at reflux, the reaction was diluted with water until a precipitate started to form. The mixture was cooled 29 585152 to room temperature and filtered. The residue was washed with water to give 4.64 g (79%) of a brown solid. MS m/z 288 (M++1).

EXAMPLE 13 Reduction of Ketone 17 to Alcohol 18 (R? = 2-furvl. R-3 =H.Ra= Nhk R^ = CN. X = H. OH) To a 0°C solution of ketone 17 (0.115 g, 0.40 mmol) in 12 mL of THF was added a 1.0 M LiAIH4 solution in THF (0.40 mL, 0.40 mmol). The 10 reaction was stirred at 0°C for 1 h. The reaction was quenched by the addition of ethyl acetate (1.5 mL), water (1.5 mL), 10% aq. NaOH (1.5 mL), and saturated aq. NH4CI (3.0 mL). The mixture was extracted with ethyl acetate (3 x 35 mL), washed with brine, and dried over sodium sulfate. The remaining solution was concentrated to yield 0.083 g (72%) of a yellow solid. 15 MS m/z 290 (M++1).

EXAMPLE 14 Hydrolysis of Nitrile 17 to Carboxylic Acid 19 (R? = 2-furvl. R3 = H. R4 = NH?, R* = COOH. X = O) To a mixture of nitrile 17 (0.695 g, 2.42 mmol) and ethanol (30 mL) was added 5 mL of 35% aqueous sodium hydroxide. The resulting mixture was heated to reflux overnight. After cooling to rt, the solution was poured into water and acidified with 1 N HCI. The resulting precipitate was isolated 25 by filtration and washed with water to yield 0.623 g (84%) of a brown solid. MS m/z 329 (M++23).

EXAMPLE 15 Synthesis of Carboxylic Ester 20 with Silver Carbonate 30 (R? = 2-furvl. R3 = H, R4 = NH?, R* = CO?CH?CH?NMe?, X = O) A suspension of carboxylic acid 19 (5.0 g, 16.3 mmol), silver carbonate (5.8 g, 21.2 mmol), and tetrabutylammonium iodide (1.5 g, 4.1 mmol) in 80 mL of DMF was heated to 90°C. After 1 h, the mixture was cooled to rt and 585152 2-(dimethylamino)ethylchloride hydrochloride (2.4 g, 16.3 mmol) was added and the mixture was heated to 100°C. After 7 h, the reaction was filtered while hot, concentrated and purified by column chromatography eluting with 0-10% MeOH/CH2CI2 to yield 0.160 g (3%) of a yellow solid. MS m/z 378 5 (M++1). The product was dissolved in a minimum of dichloromethane and treated with 1.0 M HCI in ether to obtain the hydrochloride salt.

EXAMPLE 16 Synthesis of Carboxylic Ester 20 with DEPC 10 (R? = 2-furvl. Ra = H. Ra = NH?. = CQ9CH9CH(-CH9CH?CH9(Me)N-).

X = Q) To a mixture of carboxylic acid 19 (0.40 g, 1.3 mmol) and (S)-l-methyl-2-pyrrolidinemethanol (0.50 mL, 3.9 mmol) in DMF (30 mL) was added 0.20 mL (1.3 mmol) of diethylphosphoryl cyanide and triethylamine (0.20 mL, 1.3 15 mmol). The reaction was stirred at 0°C for one hour and then heated up to approximately 70°C overnight. The reaction was then cooled to rt and diluted with ethyl acetate. The organic mixture was washed with saturated aqueous NaHC03, water, and brine. After being dried with sodium sulfate, the solution was concentrated. The residue was purified by column chromatography 20 eluting with 10-100% ethyl acetate in hexane and then preparative TLC eluting with 2% MeOH in dichloromethane to yield 1.9 mg (0.4%) of a yellow solid. MS m/z 404 (M++1).

EXAMPLE 17 Synthesis of Carboxylic Amide 21 with DEPC (R? = 2-furvl. R3 = H. Ra = NH?, R^ = C09CH9CH(-CH9CH?CH9(Me)N-). X = O) To a mixture of carboxylic acid 19 (0.25 g, 0.82 mmol) and N,N,N'-trimethylethylenediamine (0.14 mL, 1.08 mmol) in DMF (20 mL) was added 30 0.12 mL (0.82 mmol) of diethylphosphoryl cyanide and triethylamine (0.11 mL, 0.82 mmol). The reaction was stirred at 0°C for one hour and then heated up to approximately 60°C overnight. The reaction was then cooled to rt and diluted with ethyl acetate. The organic mixture was washed with saturated aqueous NaHC03, water, and brine. After being dried with 31 585152 magnesium sulfate, the solution was concentrated. The residue was purified by column chromatography eluting with 0-10% methanol in dichloromethane and then preparative TLC eluting with 1% MeOH in dichloromethane to yield 3.3 mg (10%) of a yellow solid. MS m/z 391 (M++1). The product was 5 dissolved in a minimum of diethyl ether and treated with 1.0 M HCI in ether to obtain the hydrochloride salt.

EXAMPLE 18 Synthesis of Carboxylic Amide 21 with EDCI 10 (R? = 2-furvl. = H. Ra = NH?. Rr = CONf-CH^^NMeC^CH?-). X = O) A mixture of carboxylic acid 19 (0.300 g, 0.979 mmol), N-methylpiperazine (0.295 g, 2.94 mmol), 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (0.563 g, 2.94 mmol) 1-hydroxybenzotriazole 15 hydrate (0.397 g, 2.94 mmol), triethylamine (0.298 g, 2.94 mmol) in DMF (8 mL) was stirred at rt overnight. The mixture was then diluted with water and extracted several times with ethyl acetate. The combined organics were washed twice with brine and then dried over sodium sulfate. The solution was concentrated and then purified by column chromatography to afford 20 0.092 g (2%) of solid. MS m/z 389 (M++1). The product was treated with 1.0 M HCI in ether to obtain the hydrochloride salt.

EXAMPLE 19 Synthesis of Carboxylic Ester 20 via a dibromoalkane 25 (R, = Ph. R^ = H. Ra = NH9. R^ = CCbCH? C^C^NMe?. X = O) To a solution of carboxylic acid 19 (0.100 g, 0.32 mmol) in DMF (1.5 mL) was added 60% NaH dispersion in mineral oil (0.013 g, 0.32 mmol).

After 10 min at rt, 1,3-dibromopropane (0.035 mL, 0.35 mmol) was added 30 and the solution was stirred at rt for 17 h. After concentration, the residue was purified via column chromatography eluting with 40% ethyl acetate in hexanes to yield 0.014 g (9%) of a yellow solid. MS m/z 437 (M++1).

To a solution of the yellow solid (0.014 mg, 0.03 mmol) in a sealed tube was added a 40% aqueous solution of dimethylamine (0.5 mL, 3.0 32 585152 mmol). The tube was heated to 75°C for 2 h before concentrating. The residue was purified by column chromatography eluting with 0-10% methanol in dichloromethane to yield 0.009 g (70%) of a yellow solid. MS m/z 402 (M++1). The productwas dissolved in a minimal amount of CH2CI2 and 5 treated with 1 N HCI in ether to obtain the hydrochloride salt.

Following the general synthetic procedures outlined above and in Examples 1-19, the compounds of Table 1 below were prepared. 0 Table 1 No.

R2 R3a R3b R3C R3d R4 X MS (M+1) 1 4-MeOPh H h H H nh2 ch2 290 2 4-MeOPh H h H H nh2 CO 304 3 2-furvl H H H H nh2 CO 264 4 2-furvl H H H H nh2 ch2 250 3-pvridvl H H H H nh2 CO 297 (+Na) 6 4-pyridyl H H H H nh2 CO 275 7 X~N C3H2NS H H H H nh2 CO 281 8 4-CIC6H4 H H H H nh2 CO 308 9 3-N02C6H4 H H H H nh2 CO 319 Ph H H H H nh2 CO 274 33 585152 11 3-MeOCeH4 H h H H nh2 CO 304 12 2-MeOC6H4 H H H H nh2 CO 304 13 3-HOC6H4 H H H H nh2 CO 290 14 2-thiophenyl H h H H nh2 CO 302 3-thiophenyl H h H H nh2 CO 302 16 2-furyl H Br H H nh2 CO 342 17 2-furyl OH h H H nh2 CO 280 18 SCH3 nh2 h H H nh2 CO 259 19 3-FCsH4 H h H H NCHNMe2 CO 347 2-furyl nh2 h H H nh2 CO 279 21 2-furyl H h H nh2 nh2 CO 279 22 2-furyl H cf3 H H nh2 CO 332 23 2-furyl H h CO LL o H nh2 CO 332 24 Ph H h H H NHMe CO 288 2-furyl H CI CI H nh2 CO 332 26 2-furyl CI h h CI nh2 CO 332 27 Ph H h H H N(CH2)2NEt2 CO 373 28 3,4-F2C6H3 H h H H nh2 CO 310 29 3,5-F2C6H3 H h H H nh2 CO 310 O" 0 i CeHeNO h h H H nh2 CO 305 31 3,4,5-F3C2H2 H h H H nh2 CO 340 (M+Na) HO OH 32 Ph c3h7o2 h H H nh2 CO 348 HQ OH 33 Ph H h H c3h7o2 nh2 CO 348 6n 34 c8h10no H h H H nh2 CO 333 34 585152 2-furvl H H Br H nh2 CO 342/344 36 2-furvl H H H F nh2 CO 282 37 2-furvl MeO H H H nh2 CO 294 38 4-FCsH4 H h H H nh2 CO 292 39 3-FCsH4 H H H H nh2 CO 292 40 S02Me H H H H nh2 CO 298 41 Sme H H H H nh2 CO 266 42 Ome H H H H nh2 CO 477 (2M+Na) 43 NHPh H H H H nh2 CO 289 44 3-furyl H h H H nh2 CO 264 45 -methvl-2-furvl H H H H nh2 CO 278 46 2-furyl 0CH2CH2NHC02fBu H H H nh2 CO 437 47 Ph H h H H Me CO 297 48 Ph H H H H OMe CO 291 49 Ph CH2NMeCH2CH2NMe2 H H H nh2 CO 388 50 Ph ^me cehunj H H H nh2 CO 386 51 Ph c5h10no h H H nh2 CO 373 52 Ph CH2NEt2 h H H nh2 CO 359 53 Ph ax) C6Hi2n H H H nh2 CO 371 54 Ph co2m6 ■^6 cshmnoj H H H nh2 CO 429 55 Ph n -^\/c°2et u c9h16n02 H H H nh2 CO 443 56 Ph CH2NMeCH2C02Me H H H nh2 CO 389 585152 57 Ph C7H14N0416 H H H nh2 co 401 58 Ph H H H nh2 co 416 59 Ph Ac C7H13N2O H H H nh2 co 414 60 Ph N H c„h21n2o2 H H H nh2 co 486 61 Ph Et li^N C9H13N2 H H H nh2 co 422 62 Ph " i u CyHioNO H H H nh2 co 397 Table 2 36 585152 No X r2 r3a r3b r3C r3d Ri MS (M+1) 63 CO 2-furyl H H H H CN 288 64 CO Ph H H H H CN 298 65 CO Ph H H H H COOH 315 (M-1) 66 CO 3-furyl H H H H CN 288 67 CO 3-FC6H4 H H H H CN 316 68 CO 3-pyridyl H H H H CN 299 69 CO 2-furyl H H H H COOH 305 (M-1) 70 CO 2-furyl H H H H C02CH2CH2NMe2 378 71 CO 4-FC6H4 H H H H CN 316 72 CO 2-thiophenyl H H H H CN 304 73 CO 3-thiophenyl H H H H CN 304 74 CO 3-M eOC6H4 H H H H CN 328 75 CO 2-imidazolyl H H H H CN 288 76 CO 2-furyl H H H H CONHCH2CH2NMe2 377 77 CO 2-furyl H H H H CONMeCH2CH2NMe2 391 78 CO 2-furyl H H H H CONHCHMeCH2NMe2 391 79 CO 2-furyl F F F F CN 358 (M-1) 80 CO 2-furyl H H H H I O^N^ L/N, Me C6H4N02C6HiiN20 389 37 585152 81 co Ph H H H H C02CH2CH2NMe2 388 82 co 2-furyl H H H H I o^o^yX N-—/ Me C7H1 2NO2 404 83 co Ph H H H H k Me c9h17n2o2 457 84 co Ph H H H H 1^,0 c8h14no3 444 85 co Et H H H H CN 250 86 co /'-Bu H H H H CN 278 87 CO Ph H H H H C02CH2CH2CH2NMe2 402 88 CO Ph H H H H 0V--C C7H1 2NO2 414 89 CHOH 2-furyl H H H H CN 290 90 CO Ph H H H H 1 0^"0'^SY^\ N-—/ Me c7h12no2 414 91 CO Ph H H H H cr 0 ^ ^ C7H12N02 430 92 CO Ph H H H H C02CH2CHMeCH2NMe2 416 93 CO 3-thiophenyl H H H H C02CH2CH2NMe2 394 94 CO CH2CH2CHCH2 H H H H CN 276 95 CO c-Hex h H H H CN 302 (M-1) 38 585152 96 CO 2-furyl H H H H (S)-C02CHMeCH2NMe2 392 III. Biological Assays and Activity Ligand Binding Assay for Adenosine A2a Receptor Ligand binding assay of adenosine A2a receptor was performed using 5 plasma membrane of HEK293 cells containing human A2a adenosine receptor (PerkinElmer, RB-HA2a) and radioligand [3H]CGS21680 (PerkinElmer, NET1021). Assay was set up in 96-well polypropylene plate in total volume of 200 |J_ by sequentially adding 20 nl_1:20 diluted membrane, 130 (iLassay buffer (50 mM Tris-HCI, pH7.4 10 mM MgCI2, 1 mM EDTA) 10 containing [3H] CGS21680, 50 |lxL diluted compound (4X) or vehicle control in assay buffer. Nonspecific binding was determined by 80 mM NECA. Reaction was carried out at room temperature for 2 hours before filtering through 96-well GF/C filter plate pre-soaked in 50 mM Tris-HCI, pH7.4 containing 0.3% polyethylenimine. Plates were then washed 5 times with cold 50 mM Tris-HCI, 15 pH7.4, dried and sealed at the bottom. Microscintillation fluid 30 jal was added to each well and the top sealed. Plates were counted on Packard Topcount for [3H], Data was analyzed in Microsoft Excel and GraphPad Prism programs. (Varani, K.; Gessi, S.; Dalpiaz, A.; Borea, P.A. British Journal of Pharmacology, 1996, 117, 1693) Adenosine A2a Receptor Functional Assay CHO-K1 cells overexpressing human adenosine A2a receptors and containing cAMP-inducible beta-galactosidase reporter gene were seeded at 40-50K/well into 96-well tissue culture plates and cultured for two days. On 25 assay day, cells were washed once with 200 |^L assay medium (F-12 nutrient mixture/0.1% BSA). For agonist assay, adenosine A2a receptor agonist NECA was subsequently added and cell incubated at 37°C, 5% CO2 for 5 hrs before stopping reaction. In the case of antagonist assay, cells were incubated with antagonists for 5 minutes at R.T. followed by addition of 50 30 nM NECA. Cells were then incubated at 37°C, 5% CO2 for 5 hrs before stopping experiments by washing cells with PBS twice. 50 |o.L 1X lysis buffer 39 585152 (Promega, 5X stock solution, needs to be diluted to 1X before use) was added to each well and plates frozen at -20°C. For p-galactosidase enzyme colorimetric assay, plates were thawed out at room temperature and 50 |iL 2X assay buffer (Promega) added to each well. Color was allowed to develop 5 at 37°C for 1 h or until reasonable signal appeared. Reaction was then stopped with 150 jlxL 1M sodium carbonate. Plates were counted at 405 nm on Vmax Machine (Molecular Devices). Data was analyzed in Microsoft Excel and GraphPad Prism programs. (Chen, W.B.; Shields, T.S.; Cone, R. D. Analytical Biochemistry, 1995, 226, 349; Stiles, G. Journal of Biological 10 Chemistry, 1992, 267, 6451) Haloperidol-induced catalepsy study in C57bl/6 mice Mature male C57bl/6 mice (9-12 week old from ACE) were housed two per cage in a rodent room. Room temperature was maintained at 64-79 degrees and humidity at 30-70% and room lighting at 12 hrs light/12 hrs dark 15 cycle. On the study day, mice were transferred to the study room. The mice were injected subcutaneously with haloperidol (Sigma H1512, 1.0 mg/ml made in 0.3% tartaric acid, then diluted to 0.2 mg/ml with saline) or vehicle at 1.5 mg/kg, 7.5ml/kg. The mice were then placed in their home cages with access to water and food. 30 minutes later, the mice were orally dosed with 20 vehicle (0.3% Tween 80 in saline) or compounds at 10 mg/kg, 10 ml/kg (compounds, 1 mg/ml, made in 0.3% Tween 80 in saline, sonicated to obtain a uniform suspension). The mice were then placed in their home cages with access to water and food. 1 hour after oral dose, the catalepsy test was performed. A vertical metal-wire grid (1.0 cm squares) was used for the test. 25 The mice were placed on the grid and given a few seconds to settle down and their immobility time was recorded until the mice moved their back paw(s). The mice were removed gently from the grid and put back on the grid and their immobility time was counted again. The measurement was repeated three times. The average of three measurements was used for data analysis. 30 Compound 70 showed 87% inhibition and compound 3 showed 90% inhibition of haloperidol-induced catalepsy when orally dosed at 10 mg/kg.

Table 5 40 585152 No.

Ki (nM) A2a binding A2a antagonist function A1 antagonist function 1 44.64 233.7 52.98 2 2.032 6.868 .32 3 0.26 0.0066 0.288 4 0.885 2.63 .57 .355 9.64 27.1 6 3.9 4.56 16.44 7 0.26 0.49 6.89 8 58.41 .5 11.59 9 .82 4.85 7.69 6.1 0.109 1.2 11 8.85 1.63 2.47 12 33.49 32.52 172.3 13 .16 .59 .35 14 2.19 0.59 3.19 3.23 0.258 3.46 16 1.75 0.169 .22 17 6.3 67.14 111.29 18 317.95 >3000 188.99 19 110.73 .88 21.64 0.05 0.126 0.91 21 0.376 0.053 3.51 22 14.16 0.055 2.75 23 13.58 0.55 1.47 24 .32 >3000 .99 172.85 .69 17.44 26 34.57 0.88 3.13 41 585152 27 146.84 68.28 >1000 28 48.9 3.53 .86 29 .95 1.42 4.27 31.55 .15 4.05 31 140.68 .22 17.5 32 3.55 0.634 9.89 33 0.175 0.34 0.021 34 560.13 3.49 0.265 7.09 36 4.37 0.052 2.52 37 2.86 0.143 3.07 38 2.34 0.956 9.44 39 4.92 0.926 2.31 40 2720.46 41 88.01 575.43 >3000 42 118.2 782.18 >10000 43 39.9 3.68 2.34 44 3.93 0.208 7.4 45 4.013 0.005 0.016 46 60.56 490.14 32.54 47 1076.76 48 470.84 >1000 >1000 49 51.12 40.13 119.03 50 80.15 11.31 94.24 51 36.81 3.26 32.92 52 94.41 18.33 107.17 53 64.15 14.25 40.82 54 40.79 3.19 19.56 55 32.82 .84 19.86 42 585152 56 .72 6.81 .76 57 34.02 .93 39.29 58 .65 11.65 60.99 59 40.79 7.94 34.11 60 34.29 61 29.83 62 58.39 63 0.59 0.0002 0.18 64 13.09 0.138 4.61 65 574.71 244.96 163.36 66 4.21 0.069 .59 67 13.4 0.618 4.37 68 7.59 0.73 34.84 69 2261 90.16 >1000 70 9.89 0.44 .13 71 17.24 3.39 2.42 72 12.64 2.54 6.24 73 4.925 0.06 9.7 74 14.67 .7 7.28 75 23.72 1.51 78.33 76 33.03 22.13 >500 77 6.254 0.68 >500 78 17.65 1.58 >500 79 8.03 12.48 >1000 80 69.08 .86 55.99 81 228.7 29.03 33.63 82 .24 1.36 29.58 83 200.06 74.87 117.05 84 173.98 24.71 27.42 43

Claims (1)

1. 585152 85 507.72 86 244.07 >1000 39.26 87 98.93 39.45 >300 88 129.6 48.87 >300 89 5.85 1.12 11.16 90 202.17 57.7 >300 91 208.32 22.07 14.67 92 38.82 13.9 32.88 93 64.05 23.57 104.31 94 49.55 >1000 35.99 95 338.13 >1000 110.22 96 48.55 10.08 52.45 44 585152 CLAIMS What is claimed is: 1. A compound having the structure of Formula I Formula I or a pharmaceutically acceptable salt thereof, wherein (a) R2 is selected from the group consisting of optionally substituted aryl and optionally substituted heteroaryl; (b) R3 is from one to four groups independently selected from the group consisting of: hydrogen, halo, Ci_s straight or branched chain alkyl, arylalkyl, C3.7 cycloalkyl, C1-8 alkoxy, cyano, C1.4 carboalkoxy, trifluoromethyl, Ci_8 alkylsulfonyl, halogen, nitro, hydroxy, trifluoromethoxy, Ci_s carboxylate, aryl, heteroaryl, and heterocyclyl, -NR11R12, wherein R-n and R-12 are independently selected from H, C*i_8 straight or branched chain alkyl, arylalkyl, C3.7 cycloalkyl, carboxyalkyl, aryl, heteroaryl, and heterocyclyl or Rioand Rn taken together with the nitrogen form a heteroaryl or heterocyclyl group, -NR*|3COR*|4, wherein R13 is selected from hydrogen or alkyl and R14 is selected from hydrogen, alkyl, substituted alkyl, C1.3 alkoxyl, carboxyalkyl, aryl, arylalkyl, heteroaryl, heterocyclyl, Ri5RieN (CH2)P-, or Ri5Ri6NCO(CH2)p-, wherein R15 and Ri6 are independently selected from H, OH, alkyl, and alkoxy, and p is an integer from 1-6, 45 585152 wherein the alkyl group may be substituted with carboxyl, alkyl, aryl, substituted aryl, heterocyclyl, substituted heterocyclyl, heteroaryl, substituted heteroaryl, hydroxamic acid, sulfonamide, sulfonyl, hydroxy, thiol, alkoxy or arylalkyl, or Ri3and Retaken together with the carbonyl form a carbonyl containing heterocyclyl group; (c) R4 is selected from the group consisting of hydrogen, Ci.6 straight or branched chain alkyl and benzyl, wherein the alkyl and benzyl groups are optionally substituted with one or more groups selected from C3.7 cycloalkyl, C-i_8 alkoxy, cyano, C-i_4 carboalkoxy, trifluoromethyl, Ci_8 alkylsulfonyl, halogen, nitro, hydroxy, trifluoromethoxy, C-i_8 carboxylate, amino, NR^Ris, aryl and heteroaryl, -OR-I7, and — NR17R1S, wherein R17 and Ri8 are independently selected from hydrogen, and optionally substituted Ci_6 alkyl or aryl; and (d) X is selected from C=S, C=0; CH2, CHOH, CHOR19; or CHNR20R21 where R19, R2o, and R2i are selected from optionally substituted Ci_8 straight or branched chain alkyl, wherein the substituents on the alkyl group are selected from Ci_8 alkoxy, hydroxy, halogen, amino, cyano, or NR22R23wherein R22and R23 are independently selected from the group consisting of hydrogen, Ci_8 straight or branched chain alkyl, C3.7 cycloalkyl, benzyl, aryl, heteroaryl, or NR22R23 taken together from a heterocycle or heteroaryl; wherein, unless otherwise stated, "alkyl" means straight, cyclic or branched-chain alkyl containing 1-20 carbon atoms and optionally substituted with one or more groups such as halogen, OH, CN, mercapto, nitro, amino, C1-Cg-alkyl, CrCg-alkoxyl, CrCg-alkylthio, CrCs-alkyl-amino, di(Cr C8-alkyl)amino, (mono-, di-, tri-, and per-) halo-alkyl, formyl, carboxy, alkoxycarbonyl, CrC8-alkyl-C0-0-, CrC8-alkyl-CO- 46 585152 NH-, carboxamide, hydroxamic acid, sulfonamide, sulfonyl, thiol, aryl, aryl(Ci-C8)alkyl, heterocyclyl and heteroaryl; "aryl" or "Ar", whether used alone or as part of a substituent group, is a carbocyclic aromatic radical that may be substituted by 5 independent replacement of 1 to 5 of the hydrogen atoms thereon with halogen, OH, CN, mercapto, nitro, amino, CrC8-alkyl, CrC8-alkoxyl, CrC8-alkylthio, CrC8-alkyl-amino, di(CrC8-alkyl)amino, (mono-, di,tri-, and per-) halo-alkyl, formyl, carboxy, alkoxycarbonyl, CrCg-alkyl-CO-O-, CrCs-alkyl-CO-NH-, or 10 carboxamide; and "heterocycle", "heterocyclic" and "heterocyclo" refer to an optionally substituted, fully or partially saturated cyclic group which is, for example, a 4- to 7-membered monocyclic, 7-to 11-membered bicyclic, or 10- to 15-membered tricyclic ring system, which has 15 at least one heteroatom in at least one carbon atom containing ring and wherein each ring of the heterocyclic group containing a heteroatom may have 1, 2, or 3 heteroatoms selected from nitrogen atoms, oxygen atoms, and sulfur atoms, where the nitrogen and sulfur heteroatoms may also optionally be oxidized 20 and the nitrogen atoms may optionally be quaternized; with the proviso that said compound is not: nh2 25 wherein Ar is phenyl, p-methoxyphenyl, m-methoxyphenyl, p-bromophenyl, m-bromophenyl, p-chlorophenyl, o-chlorophenyl, 1-naphthyl, 2-naphthyl, 2-thienyl or2-furanyl; or 47 585152 OH wherein Ar is p-chlorophenyl, 1-naphthyl, or 2-thienyl. A compound having the structure of Formula II or a pharmaceutically acceptable salt thereof, wherein (a) Ri is selected from the group consisting of (i) -COR5, wherein R5 is selected from H, optionally substituted Ci_s straight or branched chain alkyl, optionally substituted aryl and optionally substituted arylalkyl; wherein the substituents on the alkyl, aryl and arylalkyl group are selected from C-i_8 alkoxy, phenylacetyloxy, hydroxy, halogen, p-tosyloxy, mesyloxy, amino, cyano, carboalkoxy, or NR7R8 wherein R7 and Rs are independently selected from the group consisting of hydrogen, C^s straight or branched chain alkyl, C3.7 cycloalkyl, benzyl, aryl, or heteroaryl or NR7R8 taken together form a heterocycle or heteroaryl; (ii) COOR5, wherein R5 is as defined above; (iv) cyano; (v) -CONR9R10 wherein Rgand R10 are independently selected from H, straight or branched chain alkyl, C3. 7 cycloalkyl, trifluoromethyl, hydroxy, alkoxy, acyl, 48 585152 alkylcarbonyl, carboxyl, arylalkyl, aryl, heteroaryl and heterocyclyl; wherein the alkyl, cycloalkyl, alkoxy, acyl, alkylcarbonyl, carboxyl, arylalkyl, aryl, heteroaryl and heterocyclyl groups may be substituted with carboxyl, alkyl, aryl, substituted aryl, heterocyclyl, substituted heterocyclyl, heteroaryl, substituted heteroaryl, hydroxamic acid, sulfonamide, sulfonyl, hydroxy, thiol, amino, alkoxy or arylalkyl, or Rg and R10 taken together with the nitrogen to which they are attached form a heterocycle or heteroaryl group; (v) optionally substituted Ci_8 straight or branched chain alkyl; wherein the substituents on the alkyl, group are selected from C1-8 alkoxy, phenylacetyloxy, hydroxy, halogen, p-tosyloxy, mesyloxy, amino, cyano, carboalkoxy, carboxyl, aryl, heterocyclyl, heteroaryl, sulfonyl, thiol, alkylthio, or NR7R8 wherein R7 and R8 are as defined above; R2 is selected from the group consisting of optionally substituted alkyl, optionally substituted aryl, optionally substituted heteroaryl, optionally substituted heterocyclyl and optionally substituted C3_7 cycloalkyl, C-i_8 alkoxy, aryloxy, Ci_8 alkylsulfonyl, arylsulfonyl, arylthio, Ci_8alkylthio, or-NR24R25 wherein R24and R25 are independently selected from H, Ci_8 straight or branched chain alkyl, arylalkyl, C3_7 cycloalkyl, carboxyalkyl, aryl, heteroaryl, and heterocyclyl or R24 and R25 taken together with the nitrogen form a heteroaryl or heterocyclyl group, R3 is from one to four groups independently selected from the group consisting of: hydrogen, halo, Ci_8 straight or branched chain alkyl, arylalkyl, C3.7 cycloalkyl, C1-8 alkoxy, cyano, C<|_4 carboalkoxy, trifluoromethyl, Ci_8 alkylsulfonyl, halogen, nitro, hydroxy, 49 585152 trifluoromethoxy, Ci.8 carboxylate, aryl, heteroaryl, and heterocyclyl, -NR11R12, wherein R-n and R12 are independently selected from H, Ci_8 straight or branched chain alkyl, arylalkyl, c3.7 5 cycloalkyl, carboxyalkyl, aryl, heteroaryl, and heterocyclyl or Ri0and Rn taken together with the nitrogen form a heteroaryl or heterocyclyl group, -nr13cor14, wherein R13 is selected from hydrogen or alkyl and R14 is 10 selected from hydrogen, alkyl, substituted alkyl, C-i_3 alkoxyl, carboxyalkyl, aryl, arylalkyl, heteroaryl, heterocyclyl, R15R16N (CH2)P-, or Ri5Ri6NCO(CH2)p-, wherein R15 and Rie are independently selected from H, OH, alkyl, and alkoxy, and p is an integer from 1-6, 15 wherein the alkyl group may be substituted with carboxyl, alkyl, aryl, substituted aryl, heterocyclyl, substituted heterocyclyl, heteroaryl, substituted heteroaryl, hydroxamic acid, sulfonamide, sulfonyl, hydroxy, thiol, alkoxy or arylalkyl, or R^and Retaken together with the 20 carbonyl form a carbonyl containing heterocyclyl group; and (d) X is selected from C=S, C=0; CH2, CHOH, CHOR19; or chnr20r21 where R19, R2o, and R21 are selected from optionally substituted Ci.8 straight of branched chain alkyl, wherein the 25 substituents on the alkyl group are selected from Ci_8 alkoxy, hydroxy, halogen, amino, cyano, or NR22R23wherein R22and r23 are independently selected from the group consisting of hydrogen, Ci_8 straight or branched chain alkyl, C3.7 cycloalkyl, benzyl, aryl, heteroaryl, or nr22r23 taken together from a 30 heterocycle or heteroaryl; wherein, unless otherwise stated, "alkyl" means straight, cyclic or branched-chain alkyl containing 1-20 carbon atoms and optionally substituted with one or more groups such as halogen, OH, CN, mercapto, nitro, amino, Cr 50 585152 Cg-alkyl, CrC8-alkoxyl, CrC8-alkylthio, CrC8-alkyl-amino, di(Cr C8-alkyl)amino, (mono-, di-, tri-, and per-) halo-alkyl, formyl, carboxy, alkoxycarbonyl, CrCs-alkyl-CO-O-, CrCs-alkyl-CO-NH-, carboxamide, hydroxamic acid, sulfonamide, sulfonyl, thiol, aryl, aryl(CrC8)alkyl, heterocyclyl and heteroaryl; "aryl" or "Ar", whether used alone or as part of a substituent group, is a carbocyclic aromatic radical that may be substituted by independent replacement of 1 to 5 of the hydrogen atoms thereon with halogen, OH, CN, mercapto, nitro, amino, Ci-C8-alkyl, CrC8-alkoxyl, CrC8-alkylthio, CrCs-alkyl-amino, di(CrC8-alkyl)amino, (mono-, di,tri-, and per-) halo-alkyl, formyl, carboxy, alkoxycarbonyl, CrC8-alkyl-C0-0-, CrC8-alkyl-CO-NH-, or carboxamide; and "heterocycle", "heterocyclic" and "heterocyclo" refer to an optionally substituted, fully or partially saturated cyclic group which is, for example, a 4- to 7-membered monocyclic, 7-to 11-membered bicyclic, or 10- to 15-membered tricyclic ring system, which has at least one heteroatom in at least one carbon atom containing ring and wherein each ring of the heterocyclic group containing a heteroatom may have 1, 2, or 3 heteroatoms selected from nitrogen atoms, oxygen atoms, and sulfur atoms, where the nitrogen and sulfur heteroatoms may also optionally be oxidized and the nitrogen atoms may optionally be quaternized; with the proviso that when Ri is a cyano, then R2 is not phenyl. The compound of claim 2, wherein R2 is optionally substituted heteroaryl or optionally substituted aryl. The compound of claim 1 or claim 3, wherein R2 is an optionally substituted furan. The compound of any one of the preceding claims, wherein Ri is COOR5 and R5 is an optionally substituted C-i_8 straight or branched chain alkyl. 51 6. 7. 8. 9. 10 11 12 13 14 15 16 585152 The compound of any one of the preceding claims, wherein X is C=0. The compound of any one of claims 1 and 4 to 6, wherein the compound is of formula I and R4 is amino. The compound of Claim 1, which is 2-amino-4-furan-2-yl-indeno[1,2-d]pyrimidin-5-one. The compound of Claim 1, which is 2-amino-4-phenyl-indeno[1,2-d]pyrimidin-5-one. The compound of Claim 1, which is 2-amino-4-thiophen-2-yl-indeno[1,2-d]pyrimidin-5-one. The compound of Claim 1, which is 2-amino-4-(5-methyl-furan-2-yl)-indeno[1,2-d]pyrimidin-5-one. The compound of Claim 1, which is 2,6-diamino-4-furan-2-yl-indeno[1,2-d]pyrimidin-5-one. The compound of Claim 2, which is 9/-/-indeno[2,1-c]pyridine-4-carbonitrile, 3-amino-1 -furan-2-yl-9-oxo-. The compound of Claim 2, which is 9/-/-indeno[2,1-c]pyridine-4-carboxylic acid, 3-amino-1-furan-2-yl-9-oxo-, 2-dimethylamino-ethyl ester. The compound of Claim 2, which is 9/-/-indeno[2,1-c]pyridine-4-carboxylic acid, 3-amino-1-phenyl-9-oxo-, 2-dimethylamino-ethyl ester. The compound of Claim 2, which is 9/-/-indeno[2,1-c]pyridine-4-carboxylic acid, 3-amino1-furan-2-yl-9-oxo-, (2-dimethylamino-1-methyl-ethyl)-amide. 52 585152 17. The compound of Claim 2, which is 9/-/-indeno[2,1-c]pyridine-4-carboxylic acid, 3-amino-1-furan-2-yl-9-oxo-, (2-dimethylamino-ethyl)-methyl-amide. 5 18. The compound of Claim 2, which is 9/-/-indeno[2,1-c]pyridine-4-carboxylic acid, 3-amino-1-furan-2-yl-9-oxo-, 1-methyl-pyrrolidin-2-ylmethyl ester. 10 19. A pharmaceutical composition comprising the compound of any one of claims 1 to 18 and a pharmaceutically acceptable carrier, wherein the proviso of claim 1 does not apply. 20. The use of a compound of any one of claims 1 to 18 or a 15 pharmaceutical composition of claim 19 for the preparation of a medicament for treating a subject having a disorder ameliorated by antagonizing Adenosine A2a receptors in appropriate cells in the subject, wherein the disorder is a neurodegenerative disorder or a movement disorder, wherein the proviso of claim 1 20 does not apply. 21. The use of claim 20, wherein the disorder is selected from the group consisting of Parkinson's Disease, Huntington's Disease, Multiple System Atrophy, Corticobasal Degeneration, Alzheimer's Disease, and 25 Senile Dementia. 53