NZ580904A

NZ580904A - Amino-heterocyclic compounds for inhibiting pde9

Info

Publication number: NZ580904A
Application number: NZ580904A
Authority: NZ
Inventors: Patrick Robert Verhoest; Caroline Proulx-Lafrance
Original assignee: Pfizer
Priority date: 2007-05-11
Filing date: 2008-05-05
Publication date: 2012-02-24
Also published as: CU23834B1; GEP20125405B; WO2008139293A1; MA31373B1; DOP2009000258A; DK2152712T3; ES2377849T3; EP2152712A1; IL201977A0; TWI362262B; HRP20120105T1; CU20090184A7; BRPI0811280B8; GT200900294A; RS52166B; MX2009011830A; TN2009000471A1; CA2687035A1; PT2152712E; AR066502A1

Abstract

Disclosed are amino-heterocyclic compounds represented by the compounds of general formula (I), or pharmaceutically acceptable salts thereof, such as 6-[(3S,4S)1-benzyl-4-methylpyrrolid in-3-yl]-1-cyclopentyl-1,5-di hydro-4H-pyrazolo[3,4-d]pyrimidin-4-one. Also disclosed is the use of the compounds of formula (I) for inhibiting PDE9 and treating a neurodegenerative disease such as Alzheimer's disease.

Description

<div class="application article clearfix" id="description"> New Zealand Paient Spedficaiion for Paient Number 580904 Received at IPONZ 10 January 2012 -1- AMINO-HETEROCYCLIC COMPOUNDS RELATED APPLICATION This application claims the benefit under 35 U.S.C. §119(e) of U.S. 5 provisional application Ser. No. 60/917,333, filed June 11, 2007. FIELD OF THE INVENTION This invention relates to a series of novel compounds that are selective inhibitors of phosphodiesterase type 9 ("PDE9"). More particularly, the invention relates to pyrazolo[3,4-d]pyrimidinone compounds for use in the treatment and 10 prevention of neurodegenerative diseases and other diseases and disorders influenced by modulation of PDE9. BACKGROUND OF THE INVENTION Any discussion of the prior art throughout the specification should in no way be considered as an admission that such prior art is widely known or forms part of common 15 general knowledge in the field. Cyclic nucleotides cyclic guanosine monophosphate (cGMP) and cyclic adenosine monophosphate (cAMP) are important second messengers and thus are central to the control and regulation of a multitude of cellular events, both physiological and pathophysiological, in a wide variety of organs. 20 Cyclic GMP is formed from GTP by the catalytic reaction of guanylyl cyclase (GC), which is activated by nitric oxide (NO). Cyclic GMP in turn activates cGMP-dependent protein kinases (cGK), which mediate localized and global signaling. A variety of physiological processes in the cardiovascular, nervous and immune systems are controlled by the NO/cGMP pathway, 25 including ion channel conductance, glycogenolysis, cellular apoptosis, and smooth muscle relaxation. In blood vessels, relaxation of vascular smooth muscles leads to vasodilation and increased blood flow. The phosphodiesterase (PDE) enzyme family hydrolyses cGMP and cAMP, The PDE9 enzyme has been identified as a novel member of the PDE 30 enzyme family that selectively hydrolyses cGMP over cAMP. See Fisher et a/., Received at IPONZ 10 January 2012 -1a- J. Biol. Chem., 273(25), 15559-15564 (1998). PDE9 has been found to be present in a variety of human tissues, namely the testes, brain, small intestine, skeletal muscle, heart, lung, thymus and spleen, as well as in smooth muscle cells within the human vasculature of a variety of tissues. WO 2008/139293 PCT/IB2008/001125 Recent studies have directly implicated dysfunction of NO/cGMP/cGK signaling in Alzheimer's disease. For example, disruption of Long Term Potentiation (LTP), a physiological correlate of learning and memory, by amyloid-fi peptide was shown to result from a malfunction of NO/cGMP 5 signaling. Puzzo et ai, J. Neurosci., 25(29):6887-6897 (2005). Moreover, in rats showing deficits in memory tasks due to depletion in forebrain acetylcholinesterase (which is associated with Alzheimer's disease), administration of a nitric oxide mimetic increased GC activity and reversed the cognitive deficits in memory tasks. Bennett et a/., 10 Neuropsychopharmacology, 32:505-513 (2007). It is therefore believed that therapeutic agents capable of enhancing the GC/NO/cGMP/cGK signaling cascade may be useful as a new approach to the treatment of Alzheimer's disease and other neurodegenerative disorders. By reducing or preventing Ihe hydrolysis of cGMP by PDE9, PDE9 15 inhibitors elevate the intracellular level of cGMP, thus enhancing or prolonging its effects. It has been found that an increase in cGMP concentration in rats leads to improvement in learning and memory in social and object recognition tests. See, e.g., Boess et al., Neuropharmacology, 47:1081-1092 (2004). Inhibition of PDE9 has been shown to increase LTP. Hendrix, BMC 20 Pharmacol., 5(Supp 1 ):55 (2005). Accordingly, there is a need for PDE9 inhibitors that are effective in treating conditions that may be regulated or normalized by inhibition of PDE9. SUMMARY OF THE INVENTION 25 The present invention is directed to compounds of Formula (I), Received at IPONZ 10 January 2012 -3- (I) and pharmaceutically acceptable salts thereof, wherein R is selected from the group consisting of (Ci-C6)alkyl, (C2-C6)alkenyl, (C2-C6)alkynyl, (C3-C8)cycloatkyl( heterocycloalkyl, aryl, and heteroaryl, each of 5 which optionally may be substituted with one to three substituents, the substituents being independently selected from the group consisting of (Cr C4)alkyl, (CrC4)alkoxy, halo, and (C-i-C4)haloalkyl. Ri is selected from the group consisting of hydrogen, (Ci-C4)alkyl, (02-C4)alkenyl, (C2-C4)alkynyl, (Ci-C4)haloalkyl, and cyclopropyl; 10 R2 is selected from the group consisting of (Ci-C6)alkyl, (C2-C6)alkenyl, (C2-C6)alkynyl, (Ci-CeJhaloalkyl, heteroary! selected from the group consisting of pyridinyl, pyridazinyl, pyrimidinyl, and pyrazinyl, and ER5, wherein the heteroaryl optionally may be substituted with one to three substituents independently selected from the group consisting of (Ci-C4)alkyl and (CrC4)haloalkyl; 15 R3 is selected from the group consisting of hydrogen, (Ci-C4)alkyl, (C2- C4)alkenyl, (C2-C4)alkynyl, (C3-C6)cycloalkyl, and (Ci-C4)haloalkyl; E is selected from the group consisting of-CH2~ -CH2CH2-, -CH2CH2CH2- and -C(O)-; Rs is selected from the group consisting of (C3-Cg)cycloalkyl, heterocycloalkyl, 20 aryl, aryloxy, and heteroaryl, any of which optionally may be substituted with one to three substituents, such substituents being independently selected from the group consisting of (Ci-C4)alkyl, (C2-C4)alkenyl, (C2-C4)alkynyl, (CrC4)hydroxyalkyl, (Cr C4)haloalkyl, (Ci-C4)alkoxy, (Ci-C4)haloalkoxy, (C3-C8)cycloalkyl, halo, cyano, phenyl, morpholinyl, (CrC4)alkylamino, pyrazolyl, triazolyl, and imidazolyl, Received at IPONZ 10 January 2012 The present invention is also directed to compositions containing a compound of Formula (I), or a pharmaceutically acceptable salt thereof, and a pharmaceutically acceptable vehicle, carrier or diluent, and optionally further comprising a second pharmaceutical agent. The present invention is further directed to a method of inhibiting PDE9 in a mammal in need of such inhibition, comprising the step of administering to the mammal a PDE9-inhibiting amount of a) a compound of Formula I, or a pharmaceutically acceptable salt thereof; or b) a pharmaceutical composition comprising a compound of Formula I, or a pharmaceutically acceptable salt thereof, and a pharmaceutically acceptable vehicle, carrier or diluent. According to a second aspect, the present invention provides a compound of Formula (I), or a pharmaceutically acceptable salt thereof. According to a third aspect, the present invention provides a compound which is 6-[(3S,4S)-4-methyl-1-(pyridin-2-ylmethyl)pyrro!idin-3-yl]-1-(tetrahydro-2H-pyran-4-yl)-1l5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one. According to a fourth aspect, the present invention provides a pharmaceutical composition comprising a compound of the first to third aspects, or a pharmaceutically acceptable salt thereof, and a pharmaceutically acceptable vehicle, carrier or diluent According to a fifth aspect, the present invention provides use of a compound of any one of the first to third aspects, or a pharmaceutically acceptable salt thereof, in the manufacture of a medicament for inhibiting PDE9 in a mammal in need of such inhibition. Received at IPONZ 10 January 2012 -4a- 10 15 According to a sixth aspect, the present invention provides use of a compound of any one of the first to third aspects, or a pharmaceuticaily acceptable salt thereof, in the manufacture of a medicament for treating a neurodegenerative disease in a mammal in need of such treatment. According to a seventh aspect, the present invention provides use of a compound of any one of the first to third aspects, or a pharmaceutically acceptable salt thereof, in the manufacture of a medicament for promoting neurorestoration in a mammal in need of such neurorestoration. According to an eighth aspect, the present invention provides use of a compound of any one of the first to third aspects, or a pharmaceutically acceptable salt thereof, in the manufacture of a medicament for improving cognitive deficits in a mammal in need of such improvement. With the foregoing and other advantages and features of the invention that will become hereinafter apparent, the nature of the invention may be more clearly understood by reference to the following detailed description of the invention and the appended claims. DETAILED DESCRIPTION OF THE INVENTION The present invention comprises novel selective PDE9 inhibitors of 20 Formula (I), 25 («) and pharmaceutically acceptable salts thereof, wherein: R is selected from the group consisting of (Ci-C6)alkyl, (C2-C6)alkenyl, (C2-C5)alkynyl, (Ca-Csjcycloalkyl, heterocycloalkyl, aryl, and heteroaryl, each of Received at IPONZ 10 January 2012 -4b- which optionally may be substituted with one to three substituents, the substituents being independently selected from the group consisting of (Ci-C4)alkyl, (Ci-C4)alkoxy, halo, and (CrC4)haloalkyl. Ri is selected from the group consisting of hydrogen, (Ci-C4)alkyl, (C2-C4)alkenyl, (C2-C4)alkynyl, (Ci-C4)haloaIkyl, and cyclopropyl; R2 is selected from the group consisting of (Ci-C6)alkyl, (C2-C6)alkenyl, (C2-C6)alkynyl, (Ci-C6)haIoaIkyl, heteroaryl selected from the group consisting of pyridinyl, pyridazinyl, pyrimidinyl, and pyrazinyl, and ER5, wherein the WO 2008/139293 PCT/IB2008/001125 heteroaryl optionally may be substituted with one to three substituents independently selected from the group consisting of (Ci-C4)alkyl and (Cr C4)haloalkyl; R3 is selected from the group consisting of hydrogen, (CrC4)alkyl, (C2-5 C^Jalkenyl, (C2-C<))alkynyll (C3-C6)cycloalkyl, and (Ci-C4)haloalkyl; E is selected from the group consisting of -CH2-, -CH2CH2-, -CH2CH2CH2-, and -C(O)-; R5 is selected from the group consisting of (C3-Ce)cycloalkyl, heterocycloalkyl, aryl, aryloxy, and heteroaryl, any of which optionally may be 10 substituted with one to three substituents, such substituents being independently selected from the group consisting of (CVC^alkyl, (C2-C4)alkenyl, (C2-C4)alkynyl, (CrC4)hydroxyalkyl, (CrC4)haloalkyl, (Cr C4)alkoxy, (CrC4)haloalkoxy, (C3-C8)cycloalkyl, halo, cyano, phenyl, morpholinyl, (Ci-C4)alkylamino, pyrazolyl, triazolyl, and imidazolyl. 15 Preferably, R is selected from the group consisting of ethyl, isopropyl, trifluoroethyl, cyclobutyl, cyclopentyl, difluorocyclohexyl, methoxyphenyl, and tetrahydro-2H-pyran-4-yl; Ri is hydrogen or methyl; R2 is methyl, trifluoroethyl, trifluorobutyl, pyrimidinyl, trifluoromethylpyrimidinyl, orER5; R3 is methyl, ethyl, isopropyl, trifluoromethyl, trifluoroethyl, or cyclopropyl; E is -CH2- or -C(O)- ; 20 and R5 is selected from the group consisting of substituted or unsubstituted cyclopentyl, morpholinyl, phenyl, naphthyl, benzyloxy, pyrimidinyl, pyridinyl, quinolinyl, quinoxalinyl, pyrazinyl, pyrazolyl, benzimidazolyl, cinnolinyl, naphthydrinyl, pyrido[2,3-b]pyrazinyl, imidazo[4,5-c]pyridinyl, benzothiadiazolyl, tetrahydropyrazolo[1,5-a]pyridinyl, dihydrobenzodioxinyl, 25 imidazolyl, dihydrobenzofuranyl, triazolyl, oxazolyl, isoxazolyl, benzodioxinyl, thiazoiyl, imidazo[1,2-a]pyridinyl, tetrahydrobenzothiazoiyi, dihydrobenzoxazinyl, tetrahydropyranyl, tetrahydropyrazolo[1,5-a]azepinyl, and dihydropyrrolo[1,2-b]pyrazolyl. More preferably, R is selected from the group consisting of isopropyl, 30 cyclobutyl, cyclopentyl, and tetrahydro-2H-pyranyl; R, is hydrogen; R2 is ER5; R3 is methyl or ethyl; E is -CH2-; and R5 is selected from the group WO 2008/139293 PCT/IB2008/001125 consisting of phenyl, pyrimidin-2-yl, pyridin-2-yl, pyrazin-2-yl, and 5-methylpyrazin-2-yl. In other preferred embodiments, the compound is: 6-[(3,4-trans)-1-benzyl-4-methylpyrrolidin-3-yl]-1-(2-methoxyphenyl)-5 1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one 6-[(3,4-trans)-1-benzyl-4-methylpyrrolidin-3-yl]-1 -cyclopentyl-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 6-[(3S,4S)1 -benzyl-4-methylpyrrolidin-3-yi]-1 -cyclopentyl-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 10 1-cyclopentyl-6-[(3,4-trans)-4-methyl-1-pyrimidin-2-ylpyrrolidin-3-yl]-1,5- dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 1-cyclopentyl-6-{(3,4-trans)-4-methyl-1-[4-(trifluoromethyl)pyrimidin-2-yl]pyrroiidin-3-yl}-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 6-[(3,4-trans)-1-benzoyl-4-methylpyrrolidin-3-yl]-1-cyclopentyl-1,5-15 dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 1-cyclopentyl-6-[(3,4-trans)-4-methyl-1-(pyridin-3-ylmethyl)pyrrolidin-3-yl]-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 1-cyclopentyl-6-{(3,4-trans)-4-methyl-1-[3-(trifiuoromethyl)benzyl]pyrrolidin-3-yl}-1,5-dihydro-4H-pyrazolo[3,4-20 d]pyrimidin-4-one; 1-cyclopentyl-6-[(3,4-trans)-4-methyl-1-(quinolin-2-yimethyl)pyrrolidin-3-yl]-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 1-cyclopentyl-6-[(3,4-trans)-4-methyi-1-(quinolin-4-ylmethyi)pyrrolidin-3-yl]-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 25 1-cyclopentyl-6-[(3,4-trans)-4-methy!-1-{[6-(trifIuoromethyl)pyridin-3- yl]methyl}pyrrolidin-3-yi]-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 1 -cyclopentyl-6-[(3,4-trans)-4-methyl-1 -(quinoxalin-2-yimethyl)pyrrolidin-3-yi]-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 1 -cyclopentyl-6-[(3,4-trans)-4-methyl-1 -(quinoxalin-6-30 ylmethyl)pyrrolidin-3-yl]-1,5-dihydro-4H-pyrazoio[3,4-d]pyrimidin-4-one; WO 2008/139293 PCT/IB2008/001125 1-cyclopentyl-6-[(3,4-trans)-4-methyl-1-(pyrimidin-5-ylmethyl)pyrrolidin-3-yl]-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 1 -cyclopentyl-6-[(3,4-trans)-1,4-dimethylpyrrolidin-3-yl]-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 5 1 -cyclopentyl-6-[(3,4-trans)-4-methyl-1 -(2,2,2-trifluoroethyl)pyrrolidin-3- yl]-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 1-cyclopentyl-6-{(3,4-trans)-4-methyl-1-[(2-methylpyridin-3-yl)methyl]pyrrolidin-3-yl}-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 1-cyclopentyl-6-[(3,4-trans)-4-methyl-1-(quinolin-8-ylmethyl)pyrrolidin-3-10 yl]-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 1-cyclopentyl-6-[(3,4-trans)-4-methyl-1-(quinolin-3-ylmethyl)pyrrolidin-3-yl]-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 1 -cyclopentyl-6-{(3,4-trans)-4-methyl-1 -[(6-methylpyridin-3-yl)methyl]pyrrolidin-3-yl}-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 15 6-[(3,4-trans)-1-benzyl-4-isopropylpyrrolidin-3-yl]-1 -cyclopentyl-1,5- dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 6-[(3,4-trans)-1 -benzyl-4-methylpyrrolidin-3-yl]-1 -cyclopentyl-3-methyl-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 1-cyclapentyl-6-[(3S,4S)-4-methyl-1-(quinoxalin-6-ylmethyl)pyrrolidin-3-20 ylj-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 1-cyclopentyl-6-[(3,4-trans)-4-methyl-1-(2-phenylethyl)pyrrolidin-3-yl]-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 1-cyclopentyl-6-{(3,4-trans)-1-[(6-methoxypyridin-3-yl)methyl]-4-methylpyrrolidin-3-yl}-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 25 1-cyclopentyl-6-[(3,4-trans)-4-methyl-1-(pyridin-2-ylmethyl)pyrrolidin-3- yl]-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 1 -cyclopentyl-6-{(3,4-trans)-4-methyl-1 -[(3-methylpyridin-2-yl)methyl]pyrrolidin-3-yl}-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 6-[(3,4-trans)-1-benzyl-4-ethylpyrrolidin-3-yl]-1 -cyclopentyl-1,5-dihydro-30 4H-pyrazolo[3,4-d]pyrimidin-4-one; WO 2008/139293 PCT/IB2008/001125 6-[(3,4-trans)-1-benzyl-4-cyclopropylpyrrolidin-3-yl]-1 -cyclopentyl-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 6-[(3,4-trans)-1 -benzyl-4-methylpyrrolidin-3-yl]-1 -isopropyl-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 5 6-[(3S,4S)-1-benzyl-4-methylpyrrolidin-3-yl]-1 -isopropyl-1,5-dihydro- 4H-pyrazolo[3,4-d]pyrimidin-4-one; 1-isopropyl-6-[(3,4-trans)-4-methyl-1-(quinolin-2-ylmethyl)pyrrolidin-3-yl]-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 1-isopropyl-6-[(3,4-trans)-4-methyl-1-(quinoxalin-6-ylmethyl)pyrrolidin-10 3-yl]-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 1-isopropyl-6-[(3,4-trans)-4-methyl-1-(quinolin-3-ylmethyl)pyrrolidin-3-yl]-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 6-[(3,4-trans)-1-benzyl-4-(trifluoromethyl)pyrrolidin-3-yl]-1-cyclopentyl-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 15 1-isopropyl-6-[(3S,4S)-4-methyl-1-(quinoxalin-6-ylmethyl)pyrrolidin-3- yl]-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 1-isopropyl-6-[(3S,4S)-4-methyl-1-(quinolin-3-ylmethyl)pyrrolidin-3-yl]-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 1 -cyclopentyl-6-{(3S,4S)-4-methyl-1 -[(5-methylpyrazin-2-20 yl)methyl]pyrrolidin-3-yl}-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 6-[(3,4-trans)-1-benzyl-4-ethylpyrrolidin-3-yl]-1 -isopropyl-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 6-[(3S,4S)-1-benzyl-4-ethyipyrrolidin-3-yl]-1 -isopropyl-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 25 1-isopropyl-6-{(3S,4S)-4-methyl-1-[(2-methylpyrimidin-5- yl)methyl]pyrrolidin-3-yl}-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 6-(1-benzylpyrrolidin-3-yl)-1 -cyclopentyl-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 1-isopropyl-6-{(3S,4S)-1-[(6-methoxypyridin-3-yl)methyl]-4-30 methylpyrrolidin-3-yl}-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; WO 2008/139293 PCT/IB2008/001125 6-[(3,4-trans)-4-ethyl-1-(quinolin-3-ylmethyl)pyrrolidin-3-yl]-1-isopropyl-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 6-{(3,4-trans)-4-ethyl-1-[(6-methoxypyridin-3-yl)methyl]pyrrolidin-3-yl}-1 -isopropyl-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 5 6-[(3,4-trans)-4-ethyl-1-(quinoxalin-6-ylmethyl)pyrrolidin-3-yl]-1- isopropyl-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 1-cyclopentyl-6-{(3S,4S)-1-[(1,3-dimethyl-1H-pyrazol-5-yl)methyl]-4-methylpyrrolidin-3-yl}-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 1-cyclopentyl-6-[(3S,4S)-4-methyl-1-{4,5,6,7-tetrahydropyrazolo[1,5-10 a]pyridin-3-ylmethyl)pyrrolidin-3-yl]-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 1 -cyclopentyl-6-{(3S,4S)-4-methyl-1 -[(1 -methyl-1 H-benzimidazol-2-yl)methyl]pyrrolidin-3-yl}-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 1-isopropyl-6-{(3S,4S)-4-methyl-1-[(5-methylpyrazin-2-15 yl)methyl]pyrrolidin-3-yl}-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 6-[(3S,4S)-1-(cinnolin-3-ylmethyl)-4-methylpyrrolidin-3-yl]-1-cyclopentyl-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 1-cyclopentyl-6-[(3,4-trans)-1-(quinoxalin-6-ylmethyl)-4-(trifluoromethyl)pyrrolidin-3-yl]-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 20 1-cyclopentyl-6-{(3S,4S)-4-methyl-1-[(2-methylpyrimidin-4- yl)methyl]pyrrolidin-3-yl}-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 1-cyclopentyl-6-[(3S,4S)-1-{[2-(dimethylamino)pyrimidin-4-yl]methyl}-4-methylpyrrolidin-3-yl]-1,5-dihydro^4H-pyrazolo[3,4-d]pyrimidin-4-one; 1-cyclopentyl-6-{(3,4-trans)-4-cyclopropyl-1-[(5-methylpyrazin-2-25 yl)methyl]pyrrolidin-3-yl}-1,5-clihyclro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 1 -cyclopentyl-6-[(3,4-trans)-4-cyclopropyl-1 -(quinoxalin-6-ylmethyl)pyrrolidin-3-yl]-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 1-cyclopentyl-6-[(3,4-trans)-4-cyclopropyl-1-methylpyrrolidin-3-yl]-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 30 6-[(3,4-trans)-1-benzyl-4-methylpyrrolidin-3-yl]-1-ethyl-1,5-dihydro-4H- pyrazolo[3,4-d]pyrimidin-4-one; WO 2008/139293 PCT/IB2008/001125 -10- 6-{(3,4-trans)-4-ethyl-1-[(2-methylpyrimidin-5-yl)methyl]pyrrolidin-3-yl}-1-isopropyl-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 6-[(3,4-trans)-1-benzyl-4-methylpyrrolidin-3-yl]-1-(tetrahydro-2H-pyran-4-yl)-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 5 6-[(3S,4S)-1-benzyl-4-methylpyrrolidin-3-yl]-1-(tetrahydro-2H-pyrari-4- yi)-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 6-[(3,4-trans)-1-benzyl-4-(2,2,2-trifluoroethyl)pyrrolidin-3-yl]-1-cyclopentyl-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 6-[(3,4-trans)-1-benzyl-4-methylpyrrolidin-3-yl]-1-(4,4-10 difluorocyclohexyl)-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 6-[(3,4-trans)-1-benzyl-4-methylpyrrolidin-3-yl]-1-(2,2,2-trifluoroethyl)-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 1-isopropyl-6-[(3S,4S)-4-methyl-1-(1,5-naphthyridin-4-yl methyl )pyrrolidin-3-yl]-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 15 1 -isopropyl-6-[(3S,4S)-4-methyl-1 -(1,8-naphthyridin-4- ylmethylJpyrrolidin-S-ylj-I.S-dihydro^H-pyrazolotS^-dlpyrimidin^-one; 1-isopropyl-6-[3S,4S)-4-methyl-1-(quinolin-4-ylmethyl)pyrrolidin-3-yl]-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 1-isopropyl-6-[(3S,4S)-4-methyl-1-(pyrido[2,3-b]pyrazin-8-20 ylmethyl)pyrrolidin-3-yl]-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 1 -isopropyl-6-{(3,4-trans)-1 -[(6-methoxy-1,5-naphthyridin-4-yl)methyl]-4-methylpyrrolidin-3-yl}-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 6-{(3,4-trans)-1-[(8-fluoroquinolin-2-yl)methyl]-4-methylpyrrolidin-3-yl}-1 -isopropyl-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 25 1 -isopropyl-6-{(v)-1 -[(6-methoxyquinolin-4-yl)methyl]-4-methylpyrrolidin- 3-yl}-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 6-[(3,4-trans)-1 -benzyl-4-methylpyrrolidin-3-yl]-1 -cyclobutyl-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 6-{(3S,4S)-4-methyl-1-[(2-methylpyrimidin-5-yl)methyl]pyrrolidin-3-yl}-1-30 (tetrahydro-2H-pyran-4-yl)-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; WO 2008/139293 PCT/IB2008/001125 -11- 6-{(3,4-trans)-4-ethyl-1-[(2-methylpyrimidin-5-yl)methyl]pyrrolidin-3-yl}-1-(tetrahydro-2H-pyran-4-yl)-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 6-{(3S,4S)-4-methyl-1-[(5-methylpyrazin-2-yl)methyl]pyrrolidin-3-yl}-1-(tetrah.ydro-2H-pyran-4-yl)-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 5 6-{(3S,4S)-1-[(6-methoxypyridin-3-yl)methyl]-4-methylpyrrolidin-3-yl}-1- (tetrahydro-2H-pyran-4-yl)-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 6-[(3S,4S)-4-methyl-1-(quinolin-3-ylmethyl)pyrrolidin-3-yl]-1-(tetrahydro-2H-pyran-4-yl)-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 6-{(3S,4S)-4-methyl-1-[(2-methylpyrimidin-4-yl)methyl]pyrrolidin-3-yl}-1-10 (tetrahydro-2H-pyran-4-yl)-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 6-{(3S,4S)-4-methyl-1-[(6-methylpyridin-3-yl)methyl]pyrrolidin-3-yl}-1-(tetrahydro-2H-pyran-4-yl)-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 6-[(3S,4S)-4-methyl-1-{[6-(trifluoromethyl)pyridin-3-yl]methyl}pyrrolidin-3-yl]-1-(tetrahydro-2H-pyran-4-yl)-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-15 one; 6-{(3S,4S)-4-methyl-1-[(1 -methyl-1 H-imidazo[4,5-c]pyridin-2-yl)methyl]pyrrolidin-3-yl}-1-(tetrahydro-2H-pyran-4-yl)-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 6-{(3S ,4S)-1 -[(1,3-dimethyl-1H-pyrazol-5-yl)methyl]-4-methylpyrrolidin-20 3-yl}-1-(tetrahydro-2H-pyran-4-yl)-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 1-cyclobutyl-6-{(3,4-trans)-4-methyl-1-[(2-methylpyrimidin-5-yl)methyl]pyrrolidin-3-yl}-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 6-[(3S,4S)-1-(2,1,3-benzothiadiazol-5-ylmethyl)-4-methylpyrrolidin-3-25 ylj-1 -(tetrahydro-2H-pyran-4-yl)-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 6-[(3S,4S)-4-methyl-1-(quinoxalin-2-ylmethyl)pyrrolidin-3-yl]-1-(tetrahydro-2H-pyran-4-yl)-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 6-[(3S ,4S )-4-methyl-1 -(qui no in-4-yl methyl)pyrrolidi n-3-yl]-1 -(tetrahydro-30 2H-pyran-4-yl)-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; WO 2008/139293 PCT/IB2008/001125 6-[(3S,4S)-4-methyl-1-(pyridin-2-ylmethyl)pyrrolidin-3-yl]-1-(tetrahydro-2H-pyran-4-yl)-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 6-[(3S,4S)-1-benzyl-4-methylpyrrolidin-3-yl]-3-methyl-1-(tetrahydro-2H-pyran-4-yl)-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 5 6-[(3S,4S)-1-(3-fluorobenzyl)-4-methylpyrrolidin-3-yl]-1-(tetrahydro-2H- pyran-4-yl)-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 6-[(3S,4S)-1-(35-difluorobenzyl)-4-methylpyrrolidin-3-yl]-1-(tetrahydro-2H-pyran-4-yl)-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 6-{(3S,4S)-4-methyl-1-[4-(trifluoromethyl)benzyl]pyrrolidin-3-yl}-1-10 (tetrahydro-2H-pyran-4-yl)-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 6-[(3,4-trans)-1-benzyl-4-ethylpyrrolidin-3-yl]-1-(tetrahydro-2H-pyran-4-yl)-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 6-[(3S,4S)-1-benzyl-4-ethylpyrrolidin-3-yl]-1-(tetrahydro-2H-pyran-4-yl)-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 15 3-methyl-6-[(3S,4S)-4-methyl-1-(pyridin-3-ylmethyl)pyrrolidin-3-yl]-1- (tetrahydro-2H-pyran-4-yl)-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 3-methyl-6-{(3S,4S)-4-methyl-1-[(2-methylpyrimidin-5-yl)methyl]pyrrolidin-3-yl}-1-(tetrahydro-2H-pyran-4-yl)-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 20 6-{(3S,4S)-1-[(6-methoxypyridin-3-yl)methyl]-4-methy]pyrrolidin-3-yl}-3- methyl-1-(tetrahydro-2H-pyran-4-yl)-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 6-{(3S,4S)-4-methyl-1-[(6-methylpyridin-2-yl)methyl]pyrrolidin-3-yl}-1-(tetrahydro-2H-pyran-4-yl)-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 25 6-[(3S,4S)-1 -(4-fluorobenzyl)-4-methylpyrrolidin-3-yl]-1 -(tetrahydro-2H- pyran-4-yl)-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 6-[(3S,4S)-1-(2-fluorobenzyl)-4-methylpyrrolidin-3-yl]-1-(tetrahydro-2H-pyran-4-yl)-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 6-{(3S,4S)-4-methyl-1-[2-(trifluoromethyl)benzyl]pyrrolidin-3-yl}-1-30 (tetrahydro-2H-pyran-4-yl)-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; WO 2008/139293 PCT/IB2008/001125 6-[(3S,4S)-1-(2,4-difluorobenzyl)-4-methylpyrrolidin-3-yl]-1-(tetrahydro-2H-pyran-4-yl)-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 6-[(3S,4S)-1-(4-methoxybenzyl)-4-methylpyrrolidin-3-yl]-1-(tetrahydro-2H-pyran-4-yl)-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 5 6-[(3S,4S)-1-benzyl-4-methylpyrrolidin-3-yl]-1-(tetrahydro-2H-thiopyran- 4-yl)-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 6-[(3S,4S)-1-(2-methoxybenzyl)-4-methylpyrrolidin-3-yl]-1-(tetrahydro-2H-pyran-4-yl)-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 6-[(3S,4S)-1-(3-methoxybenzyl)-4-methylpyrrolidin-3-ylj-1-(tetrahydro-10 2H-pyran-4-yl)-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 6-{(3S,4S)-4-methyl-1-[3-(trifluoromethyl)benzyl]pyrrolidin-3-yl}-1-(tetrahydro-2H-pyran-4-yl)-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 6-[(3S,4S)-1-(26-difluorobenzyl)-4-methylpyrrolidin-3-yl]-1-(tetrahydro-2H-pyran-4-yl)-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 15 6-{(3S,4S)-4-ethyl-1-[(5-methylpyrazin-2-yl)methyl]pyrrolidin-3-yl}-1- (tetrahydro-2H-pyran-4-yl)-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 6-{(3S,4S)-4-ethyl-1-[(6-methoxypyridin-3-yl)methyl]pyrrolidin-3-yl}-1-(tetrahydro-2H-pyran-4-yl)-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 6-[(3S,4S)-4-ethyl-1-(pyridin-2-ylmethyl)pyrrolidin-3-yl]-1-(tetrahydro-20 2H-pyran-4-yl)-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 6-[(3S,4S)-4-ethyl-1-(quinoxalin-2-ylcarbonyl)pyrrolidin-3-yl]-1-(tetrahydro-2H-pyran-4-yl)-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 6-[(3S,4S)-4-methyl-1-(pyrimidin-2-ylmethyl)pyrrolidin-3-yl]-1-(tetrahydro-2H-pyran-4-yl)-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 25 2-({(3S,4S)-3-ethyl-4-[4-oxo-1-(tetrahydro-2H-pyran-4-yl)-45-dihydro- 1H-pyrazolo[3,4-d]pyrimidin-6-yl]pyrrolidin-1-yl}methyl)benzonitrile; 3-({(3S,4S)-3-ethyl-4-[4-oxo-1-(tetrahydro-2H-pyran-4-yl)-45-dihydro-1H-pyrazolo[3,4-d]pyrimidin-6-yl]pyrrolidin-1-yl}methyl)benzonitrile; 4-({(3S,4S)-3-ethyl-4-[4-oxo-1-(tetrahydro-2H-pyran-4-yl)-45-dihydro-30 1H-pyrazolo[3,4-d]pyrimidin-6-yl]pyrrolidin-1-yl}methyl)benzonitrile; WO 2008/139293 PCT/IB2008/001125 -14- 1 -cyclopentyl-6-{(3,4-trans)-4-methyl-1 -[3-(1 H-pyrazol-1 -yl)benzyl]pyrrolidin-3-yl}-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 1 -cyclopentyl-6-{(3,4-trans)-4-methyl-1 -[(2-methylpyridin-4-yl)methyl]pyrrolidin-3-yl}-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 5 6-[(3,4-trans)-1-(2-chloro-6-fliiorobenzyl)-4-methylpyrrolidin-3-yl]-1- cyclopentyl-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 1-cyclopentyl-6-[(3,4-trans)-1-(2,3-dimethylbenzyl)^-methylpyrrolidin-3-yl]-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 1-cyclopentyl-6-{(3,4-trans)-1-[2-(difluoromethoxy)benzyl]-4-10 methylpyrrolidin-3-yl}-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one 1-cyclopentyl-6-{(3,4-trans)-1-[(2-ethoxypyridin-3-yl)methyl]-4-methylpyrrolidin-3-yl}-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 1-cyclopentyl-6-[(3,4-trans)-4-methyl-1-(4,5,6,7-tetrahydropyrazolo[1,5-a]pyridin-3-ylmethyl)pyrrolidin-3-yl]-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-15 one; 1-cyclopentyl-6-[(3,4-trans)-1-(2,3-dihydro-1,4-benzodioxin-6-ylmethyl)-4-methylpyrrolidin-3-yl]-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 1-cyclopentyl-6-{(3,4-trans)-1-[4-(1H-imidazol-1-yl)benzyl]-4-methylpyrrolidin-3-yl}-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 20 1-cyclopentyl-6-[(3,4-trans)-1-(2,5-dichlorobenzyl)-4-methylpyrrolidin-3- yl]-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 1-cyclopentyl-6-[(3,4-trans)-1-(4-methoxy-3-methylbenzyl)-4-methylpyrrolidin-3-yl]-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 1-cyclopentyl-6-[(3,4-trans)-1-(2,3-dihydro-1-benzofuran-7-ylmethyl)-4-25 methylpyrrolidin-3-yl]-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 1-cyclopentyl-6-[(3,4-trans)-1-(2,3-difluorobenzyl)-4-methylpyrrolidin-3-yl]-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 1-cyclopentyl-6-[(3,4-trans)-1-(5-fluoro-2-methoxybenzyl)-4-methylpyrrolidin-3-yl]-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 30 1-cyclopentyl-6-[(3,4-trans)-1-(2-fluoro-4-methoxybenzyl)-4- methylpyrrolidin-3-yl]-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; WO 2008/139293 PCT/IB2008/001125 1-cyclopentyl-6-[(3,4-trans)-1-(3-fluoro-4-methylbenzyl)-4-methylpyrrolidin-3-yl]-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 1-cyclopentyl-6-{(3,4-trans)-4-methyl-1-[(2-methyl-1,3-thiazol-5-yl)methyl]pyrrolidin-3-yl}-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 5 1-cyclopentyl-6-{(3,4-trans)-1-[(4-isopropyl-1,3-th iazol-2-yl )methyl]-4- methylpyrrolidin-3-yl}-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 1-cyclopentyl-6-{(3,4-trans)-1-[(1,3-dimethyl-1H-pyrazol-5-yl)methyl]-4-methylpyrrolidin-3-yl}-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 1-cyclopentyl-6-[(3,4-trans)-1-(2,3-difluoro-4-methylbenzyl)-4-10 methylpyrrolidin-3-yl]-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin^4-one; 1 -cyclopentyl-6-[(3,4-trans)-4-methyl-1 -{[6-( 1 H-pyrazol-1 -yl)pyridin-2-yl]methyl}pyrrolidin-3-yJ]-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 1-cyclopentyl-6-[(3,4-trans)-4-methyl-1-(4-methylbenzyl)pyrrolidin-3-yl]-1,5-dihydro-4H-pyrazolo[3,4-d]pynmidin-4-one; 15 1-cyclopentyl-6-[(3,4-trans)-4-methyl-1-(2-naphthylmethyl)pyrrolidin-3- yl]-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 1-cyclopentyl-6-{(3,4-trans)-1-[(2-methoxypyridin-3-yl)methyl]-4-methylpyrrolidin-3-yl}-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 1-cyclopentyl-6-[(3,4-trans)-1-(2-ethoxybenzyl)-4-methylpyrrolidin-3-yl]-20 1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 1 -cyclopentyl-6-{(3,4-trans)-4-methyl-1 -[4-( 1H-1,2,4-triazol-1 -yl)benzyl]pyrrolidin-3-yl}-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 1-cyclopentyl-6-[(3,4-trans)-1-(3-methoxy-4-methylbenzyl)-4-methylpyrrolidin-3-yl]-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 25 1-cyclopentyl-6-[(3,4-trans)-4-methyl-1-(1-naphthylmethyl)pyrrolidin-3- yl]-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 1-cyciopentyl-6-[(3,4-trans)-1-(3-fluoro-4-methoxybenzyl)-4-methylpyrrolidin-3-yl]-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 1-cyclopentyl-6-[(3,4-trans)-1-(2,5-dimethoxybenzyl)-4-methylpyrrolidin-30 3-yl]-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; WO 2008/139293 PCT/IB2008/001125 1-cyclopentyl-6-{(3,4-trans)-4-methyl-1-[(5-methylisoxazol-3-yl)methyl]pyrrolidin-3-yl}-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 1-cyclopentyl-6-[(3,4-trans)-1-(2-fluoro-6-methoxybenzyl)-4-methylpyrrolidin-3-yl]-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 5 1-cyclopentyl-6-[(3,4-trans)-1-(2,4-difluorobenzyl)-4-methylpyrrolidin-3- yl]-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 1-cyclopentyl-6-[(3,4-trans)-1-(4-fluoro-3-methoxybenzyl)-4-methylpyrrolidin-3-yl]-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 1-cyclopentyl-6-[(3,4-trans)-1-(2,3-dihydro-1,4-benzodioxin-5-ylmethyl)-10 4-methylpyrrolidin-3-yl]-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 6-[(3,4-trans)-1-(2-chloro-4-fluorobenzyl)-4-methylpyrrolidin-3-yl]-1-cyclopentyl-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-cne; 1-cyclopentyl-6-[(3,4-trans)-1-(2,4-dimethylbenzyl)-4-methylpyrrolidin-3-yl]-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 15 1-cyclopentyl-6-[(3,4-trans)-1-(3,5-dimethoxybenzyl)-4-methylpyrrolidin- 3-yl]-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 1-cyclopentyl-6-[(3,4-trans)-1-(3-ethoxybenzyl)-4-methyJpyrrolidin-3-yl]-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 6-[(3,4-trans)-1-(4-chloro-2-fluorobenzyl)-4-methylpyrrolidin-3-yl]-1-20 cyclopentyl-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 3-{[(3,4-trans)-3-(1-cyclopentyl-4-oxo-4,5-dihydro-1H-pyrazolo[3,4-d]pyrimidin-6-yl)-4-methylpyrrolidin-1-yl]methyl}benzonitrile; 1-cyclopentyl-6-[(3,4-trans)-1-(2,5-difluorobenzyl)-4-methylpyrrolidin-3-yl]-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 25 2-{[(3,4-trans)-3-(1-cyclopentyl-4-oxo-4,5-dihydro-1H-pyrazolo[3,4- d]pyrimidin-6-yl)-4-methylpyrrolidin-1-yl]methyl}benzonitrile; 6-[(3,4-trans)-1-(3-chloro-4-fluorobenzyl)-4-methylpyrrolidin-3-yl]-1-cyclopentyl-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 1-cyclopentyl-6-{(3,4-trans)-1-[4-(difluoromethoxy)benzyl]-4-30 methylpyrrolidin-3-yl}-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; WO 2008/139293 PCT/IB2008/001125 -17- 1-cyclopentyl-6-[(3,4-trans)-4-methyl-1-(3-methylbenzyl)pyrrolidin-3-yl]-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 1-cyclopentyl-6-[(3,4-trans)-1-(3,4-difluorobenzyl)-4-methylpyrrolidin-3-yl]-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 5 1-cyclopentyl-6-[(3,4-trans)-1-(2,5-dimethylbenzyl)-4-methylpyrrolidin-3- yl]-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 6-[(3,4-trans)-1-(3-chloro-2-fluorobenzyl)-4-methylpyrrolidin-3-yl]-1-cyclopentyl-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 1-cyclopentyl-6-[(3,4-trans)-1-(2,3-dichlorobenzyl)-4-methylpyrrolidin-3-10 yl]-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 1 -cyclopentyl-6-[(3,4-trans)-4-methyl-1 -(1,3-thiazol-2-ylmethyl)pyrrolidin-3-yl]-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 1-cyclopentyl-6-[(3,4-trans)-1-(3-fluoro-2-methylbenzyl)-4-methylpyrrolidin-3-yl]-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 15 1-cyclopentyl-6-{(3,4-trans)-4-methyl-1-[(2-methylpyrimidin-5- yl)methyl]pyrrolidin-3-yl}-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 1-cyclopentyl-6-{(3,4-trans)-1-[(2-ethylpyrimidin-5-yl)methyl]-4-methylpyrrolidin-3-yl}-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 1-cyclopentyI-6-[(3,4-trans)-1-(4-isopropylbenzyl)-4-methylpyrrolidin-3-20 yl]-1,5-dihydro-4H-pyrazolof3,4-d]pyrimidin-4-one; 1 -cyciopentyl-6-{(3,4-trans)-1-[(1 -ethyl-1 H-pyrazol-4-yl)methyl]-4-methylpyrrolidin-3-yl}-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 1-cyclopentyl-6-{(3,4-trans)-1-[(4-methoxypyridin-3-yl)methyl]-4-methylpyrrolidin-3-yl}-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 25 1-cyclopentyl-6-[(3,4-trans)-1-(isoxazol-5-ylmethyl)-4-methylpyrrolidin- 3-yl]-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 1-cyclopentyl-6-[(3,4-trans)-1-(4-ethoxybenzyl)-4-methylpyrrolidin-3-yl]-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 1 -cyclopentyl-6-[(3,4-trans)-1-{[6-( 1 -hydroxy-1 -methylethyl)pyridin-3-30 yl]methyl}-4-methylpyrrolidin-3-yl]-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; WO 2008/139293 PCT/IB2008/001125 -18- 1-cyclopentyl-6-{(3l4-trans)-1-[(2,2-dimethyl-2,3-dihydro-1-benzofuran-5-yl)methyl]-4-methylpyrrolidin-3-yl}-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 1-cyclopentyl-6-[(3,4-trans)-1-(3,4-dimethoxybenzyl)-4-methylpyrrolidin-5 3-yl]-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 1 -cyclopentyl-6-{(3,4-trans)-4-methyl-1 -[(5-methylpyrazin-2-yl)methyl]pyrrolidin-3-yl}-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 1-cyclopentyl-6-[(3,4-trans)-1-(imidazo[1,2-a]pyridin-2-ylmethyl)-4-methylpyrrolidin-3-yl]-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 10 1-cyclopentyl-6-{(3,4-trans)-4-methyl-1-[(2-phenyl-1,3-oxazol-4- yl)methyl]pyrrolidin-3-yl}-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 1-cyclopentyl-6-[(3,4-trans)-4-methyl-1-(2-methylbenzyl)pyrrolidin-3-yl]-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 1-cyclopentyl-6-[(3,4-trans)-1-(2-isopropoxybenzyl)-4-methylpyrrolidin-15 3-yl]-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 6-[(3,4-trans)-1-(cinnolin-3-ylmethyl)-4-methylpyrrolidin-3-yl]-1-cyclopentyl-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 1-cyclopentyl-6-{(3,4-trans)-1-[3-(difluoromethoxy)benzyl]-4-methylpyrrolidin-3-yl}-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 20 1-cyclopentyl-6-[(3,4-trans)-1-(4-fluoro-3-methylbenzyl)-4- methylpyrrolidin-3-yl]-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 1 -cyclopentyl-6-{(3,4-trans)-4-methyl-1 -[4-( 1 H-pyrazol-1 -yl)benzyl]pyrrolidin-3-yl}-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 1-cyclopentyl-6-{(3,4-trans)-1-[(2,7-dimethylimidazo[1,2-a]pyridin-3-25 yl)methylJ-4-methylpyrrolidin-3-yl}-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 1-cyclopentyl-6-[(3,4-trans)-1-(3,5-dichlorobenzyl)-4-methylpyrrolidin-3-yl]-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 1-cyclopentyl-6-[(3,4-trans)-1-(4-isopropoxybenzyl)-4-methylpyrrolidin-30 3-yl]-115-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; WO 2008/139293 PCT/IB2008/001125 1 -cyclopentyl-6-[(3,4-trans)-1 -{[2-( 1 -hydroxy-1 -methylethyl)pyridin-4-yl]methyl}-4-methylpyrrolidin-3-yl]-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 1-cyclopentyl-6-[(3,4-trans)-4-methyl-1-(4,5,6,7-tetrahydro-1,3-5 benzothiazol-2-ylmethyl)pyrrolidin-3-yl]-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 1-cyclopentyl-6-[(3,4-trans)-1-(mesitylmethyl)-4-methylpyrrolidin-3-yl]-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 1-cyclopentyl-6-[(3,4-trans)-1-(2,6-dichlorobenzyl)-4-methylpyrrolidin-3-10 yl]-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 4-{[(3,4-trans)-3-(1-cyclopentyl-4-oxo-4,5-dihydro-1H-pyrazolo[3,4-d]pyrimidin-6-yl)-4-methylpyrro(idin-1-yl]methyl}benzonitrile; 1-cyclopentyl-6-[(3,4-trans)-1-(2-fluoro-5-methoxybenzyl)-4-methylpyrrolidin-3-yl]-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 15 1-cyclopentyl-6-[(3,4-trans)-1-(2,6-dimethylbenzyl)-4-methylpyrrolidin-3- yl]-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 1-cycloper)tyl-6-{(3,4-trans)-1-[(4-methoxy-3,5-dimethylpyridin-2-yl)methyl]-4-methylpyrrolidin-3-yl}-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 20 1-cyclopentyl-6-[(3,4-trans)-1-(3,5-dimethylbenzyl)-4-methylpyrrolidin-3- yl]-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 1-cyclopentyl-6-[(3,4-trans)-1-(3,4-dimethylbenzyl)-4-methylpyrrolidin-3-yl]-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 1-cyclopentyl-6-{(3,4-trans)-4-methyl-1-[(1 -methyl-1 H-benzimidazol-2-25 yl)methyl]pyrrolidin-3-yl}-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 1-cyclopentyl-6-{(3,4-trans)-4-methyl-1-[(4-methyl-3,4-dihydro-2H-1,4-benzoxazin-7-yl)methyl]pyrrolidin-3-yl}-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 1-cyclopentyl-6-[(3,4-trans)-4-methyl-1-(3-phenylpropyl)pyrrolidin-3-yl]-30 1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; WO 2008/139293 PCT/IB2008/001125 -20- 1-cyclopentyl-6-{(3,4-trans)-4-methyl-1-[2-(trifluoromethyl)benzyl]pyrrolidin-3-yl}-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 1-cyclopentyl-6-[(3,4-trans)-4-methyl-1-(4,4,4-trifluorobutyl)pyrrolidin-3-5 yl]-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 1-cyclopentyl-6-[(3,4-trans)-1-(3-methoxybenzyl)-4-methylpyrrolidin-3-yl]-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 1-cyclopentyl-6-[(3,4-trans)-1-(cyclopentylmethyl)-4-methylpyrrolidin-3-yl]-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 10 1-cyclopentyl-6-[(3,4-trans)-1-(2,4-dimethoxybenzyl)-4-methylpyrrolidin- 3-yl]-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 1-cyclopentyl-6-{(3,4-trans)-4-methyl-1-[4-(morpholin-4-ylmethyl)benzyl]pyrrolidin-3-yl}-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 6-[(3,4-trans)-1-(2,1,3-benzothiadiazol-5-ylmethyl)-4-methylpyrrolidin-3-15 yl]-1 -cyclopentyl-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 6-{(3,4-trans)-1-[2-(benzyloxy)ethyl]-4-methylpyrrolidin-3-yl}-1-cyclopentyl-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 1-cyclopentyl-6-[(3,4-trans)-1-(2,6-difluorobenzyl)-4-methylpyrrolidin-3-yl]-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 20 1-cyclopentyl-6-[(3,4-trans)-1-(2-methoxybenzyl)-4-methylpyrrolidin-3- yl]-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 1-cyclopentyl-6-{(3,4-trans)-4-methyl-1-[(3,5,6-trimethylpyrazin-2-yl)methyl]pyrrolidin-3-yl}-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 1-cyclopentyl-6-[(3,4-trans)-1-(2,4-dichlorobenzyl)-4-methylpyrrolidin-3-25 yl]-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 1 -cyclopentyl-6-[(3,4-trans)-4-methyl-1 -(5,6,7,8-tetrahydro-4H-pyrazolo[1,5-a]azepin-3-ylmethyl)pyrrolidin-3-yl]-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 1-cyclopentyl-6-[(3,4-trans)-1-(3-fluorobenzyl)-4-methylpyrrolidin-3-yl]-30 1 .S-dihydro^H-pyrazolofS^-dJpyrimidin^-one; WO 2008/139293 PCT/IB2008/001125 -21- 1 -cyclopenty[-6-[(3,4-trans)-1 -(2,3-dihydro-1 -benzofuran-5-yimethyl)-4-methy!pyrrolidin-3-ylj-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 1-cyclopentyl-6-[(3,4-trans)-1-(2-methoxy-5-methylbenzyl)-4-methylpyrrolidin-3-yl]-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 5 1-cyclopentyl-6-[(3,4-trans)-1-(2-fluorobenzyl)-4-methylpyrrolidin-3-yl]- 1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 6-[(3,4-trans)-1-(2-chlorobenzyl)-4-methylpyrrolidin-3-yl]-1-cyclopentyl-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 1-cyclopentyl-6-[(3,4-trans)-1-(3,4-dichlorobenzyl)-4-methylpyrrolidin-3-10 yl]-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 6-[(3,4-trans)-1-(2,1,3-benzothiadiazol-4-ylmethyl)-4-methylpyrrolidin-3-yl]-1 -cyclopentyl-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 1-cyclopentyl-6-{(3,4-trans)-4-methyl-1-[(2-pro pylpyrimidin-5- yl)methyl]pyrrolidin-3-yl}-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 15 1 -cyclopentyl-6-{(3,4-trans)-1-[(1 -ethyl-1 H-pyrazol-5-yl)methylj-4- methylpyrrolidin-3-yl}-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 1-cyclopentyl-6-{(3,4-trans)-4-methyl-1-[2-(trifluoromethoxy)benzyl]pyrrolidin-3-yl}-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 20 1-cyclopentyl-6-{(3,4-trans)-4-methyl-1-[4- (trifluoromethyl)benzyl]pyrrolidin-3-yl}-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 1-cyclopentyl-6-{(3,4-trans)-4-methyl-1-[(1-methyl-1 H-imidazo[4,5-c]pyridin-2-yl)methyl]pyrrolidin-3-yl}-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-25 4-one; 1-cyclopentyl-6-[(3,4-trans)-1-(3,5-difluorobenzyl)-4-methylpyrrolidin-3-yl]-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; or 1-cyclopentyl-6-[(3,4-trans)-1-(5,6-dihydro-4H-pyrrolo[1,2-b]pyrazol-3-ylmethyl)-4-methylpyrrolidin-3-yl]-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-30 one; or a pharmaceutically acceptable salt thereof. WO 2008/139293 PCT/IB2008/001125 -22- The compounds of the invention have been surprising found to show pharmacological activity including in selectively inhibiting PDE9 that makes them suitable for the treatment, prevention and/or control of in treating conditions that may be regulated or normalized by inhibition of PDE9. 5 The compounds and intermediates of the present invention may be named according to either the IUPAC (International Union for Pure and Applied Chemistry) or CAS (Chemical Abstracts Service, Columbus, OH) nomenclature systems. Definitions 10 Certain terms used herein are generally defined as follows: The carbon atom content of the various hydrocarbon-containing moieties herein may be indicated by a prefix designating the minimum and maximum number of carbon atoms in the moiety. Thus, for example, (Ci-15 C6)alkyl refers to an alkyl group of one to six carbon atoms inclusive. The term "alkoxy" refers to a straight or branched, monovalent, saturated aliphatic hydrocarbon radical bonded to an oxygen atom that is attached to a core structure. Examples of alkoxy groups include methoxy, ethoxy, propoxy, isopropoxy, butoxy, isobutoxy, fert-butoxy, pentoxy, and the 20 like. The term "alkyl" means a saturated monovalent straight or branched aliphatic hydrocarbon radical. Examples of alkyl groups include methyl, ethyl, propyl, isopropyl, butyl, isobutyl, /erf-butyl, sec-butyl, pentyl, isopentyl, neopentyl, hexyl, isohexyl, and the like. 25 The term "alkenyl" means a partially unsaturated straight or branched aliphatic hydrocarbon radical having one or more double bonds. Examples of alkenyl groups include ethenyl (also known as "vinyl"), allyl, 1-propenyl, isopropenyl, n-butenyl, n-pentenyl, and the like. The term "alkenyl" embraces radicals having "c/'s" and "trans" orientations, or alternatively, "Z' and "F 30 orientations. The term "alkynyl" means a partially unsaturated straight or branched aliphatic hydrocarbon radical having one or more double bonds. Examples of WO 2008/139293 PCT/IB2008/001125 -23- alkynyl groups include 1-propynyl, 2-propynyi (also known as "propargyl"), 1-butynyl, 2-butynyl, 1 -pentynyl, and the like. The term "aryl" denotes a monocyclic or polycyclic aromatic ring system, for example, anthracenyl, benzyl, fluorenyl, indenyl, naphthyl, 5 phenanthrenyl, phenyl and the like. The term "aryl" is also intended to include the partially hydrogenated derivatives of such ring systems, e.g. 1,2,3,4-tetrahydronaphthyl. The term "aryloxy" denotes an aryl radical bonded to an oxygen atom that is attached to a core structure, such as benzyloxy. 10 The term "cycloalkyl" denotes a saturated monocyclic or bicyclic cycloalkyl group. Examples of cycloalkyl groups include cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, cycloheptyl, cyclooctyl, and the like. The term "halogen" or "halo" represents chlorine, bromine, fluorine and iodine atoms and radicals. 15 The term "haloalkyl" refers to an alkyl or cycloalkyl substituent wherein at least one hydrogen radical is replaced with a halogen radical. Where more than one hydrogen is replaced with halogen, the halogens may be the same or different. Examples of haloalkyl radicals include trifluoromethyl, 2,2,2-trifluoroethyl, 4,4,4-trifluorobutyl, 4,4-difluorocyclohexyl, chloromethyl, 20 dichloromethyl, trichloromethyl, 1-bromoethyl, and the like. The term "haloalkoxy" refers to an alkoxy radical in which at least one hydrogen radical is replaced with a halogen radical. Where more than one hydrogen is replaced with halogen, the halogens may be the same or different. Examples of haloalkoxy radicals include difluoromethoxy, 25 trifluoromethoxy, 2,2,2-trifluoroethoxy, chloromethoxy, bromomethoxy, and the like. The term "heteroaryl" as used herein includes heterocyclic unsaturated ring systems containing one or more heteroatoms such as nitrogen, oxygen, and sulfur. If the heteroaryl group contains more than one heteroatom, the 30 heteroatoms may be the same or different. The heteroaryl radicals may be bonded via a carbon atom or a heteroatom. The term "heteroaryl" is also WO 2008/139293 PCT/IB2008/001125 -24- intended to include the partially hydrogenated derivatives of such ring systems. Examples of heteroaryl groups include furanyl (also known as "furyl"), imidazolinyl, imidazolyl (also known as "1,3-diazolyl"), indolyl, oxadiazolyl, oxazinyl, oxazolyl, isoxazoiyl, pyranyl, pyrazinyl (also known as 5 "1,4-diazinyl"), pyrazolyl (also known as "1,2-diazolyl"), pyrazolinyl, pyrazyl, pyridazinyl (also known as "1,2-diazinyl"), pyridyl (also known as pyridinyl), pyrimidinyl (also known as "1,3 diazinyl" and "pyrimidyl"), pyrrolyl, thiadiazinyl, thiadiazolyl, thiatriazolyl, thiazolyl, isothiazolyl, thienyl, thiofuranyl (also known as "thiophenyl"), thiopyranyl, triazinyl, triazolyl, and the like. 10 The term "heteroaryl" also embraces radicals in which 2 or 3 rings are fused together, wherein at least on such ring contains a heteroatom as a ring atom, including radicals wherein (a) a heterocycloalkyl ring is fused with an aryl or heteroaryl ring, or (b) a cycloalkyl ring is fused with a heteroaryl ring. Examples of 2-fused ring heteroaryls include benzodioxinyl, 15 dihydrobenzodioxinyl, benzofuranyl, dihydrobenzofuranyl, isobenzofuranyl, benzimidazolyl, benzothiadiazolyl, tetrahydrobenzothiadiazolyl, benzothiazolyl, benzothienyl (also known as "benzothiophenyl," "thionaphthenyl," and "benzothiofuranyl"), benzoxazinyl, dihydrobenzoxazinyl, benzoxazolyl, chromanyl, isochromanyl, chromenyl, cinnolinyl (also known as 20 "1,2-benzodiazinyl"), imidazopyridiny! (e.g. imidazo[1,2-a]pyridinyl or imidazo[4,5-c]pyridinyl), indazolyl, indolinyl, isoindolinyl, indolizinyl, indolyl, isoindolyl, naphthyridinyl, oxathiolopyrrolyl, pteridinyl, pthalazinyl, purinyl (also known as "imidazo[4,5-d]pyrimidinyl"), pyranopyrrolyi, pyrazoloazepinyl, tetrahydropyrazoloazepinyl (e.g. tetrahydropyrazolo[1,5-a]azepinyl), 25 pyrazolopyridinyl, tetrahydropyrazolopyridinyl (e.g. tetrahydropyrazolo[1,5-a]pyridinyl), pyrazolopyrimidinyl (e.g. pyrazolo[3,4-d]pyrimidinyl), pyridopyrazinyl (e.g. pyrido[2,3-b]pyrazinyl), pyridopyridinyl, pyrrolopyrazolyl, dihydropyrrolopyrazolyl (e.g. dihydropyrrolo[1,2-b]pyrazoiyl), quinazolinyl (also known as "1,3-benzodiazinyl"), quinoiinyl (also known as "1-benzazinyl"), 30 isoquinolinyl (also known as "2-benzazinyl"), quinolizinyl, quinolyl, isoquinolyl, WO 2008/139293 PCT/IB2008/001125 -25- quinoxalinyl, dithianaphthalenyl, thienofuranyl (e.g. thieno[3,2-b]furanyl), and the like. Examples of 3-fused ring heteroaryls include acridinyl, diazaanthryl, triazaphenanthrene, carbazolyl, carbolinyl, furocinnolinyl, perimidinyl, 5 phenanthridinyl, phenanthrolinyl, phenazinyl, phenothiazinyl, phenoxathiinyl, phenoxazinyl, thianthrenyl, xanthenyl, and the like. The term "heterocycloalkyl" denotes a saturated monocyclic or polycyclic cycloalkyl group, in which at least one of the carbon atoms is replaced with a heteroatom such as nitrogen, oxygen or sulfur. If the 10 heterocycle contains more than one heteroatom, the heteroatoms may be the same or different. The heterocycloalkyl radicals may be bonded via a carbon atom or a heteroatom. Examples of heterocycloalkyl groups include azetidinyl, dioxacyclohexyl, 1,3-dioxolanyl, imidazolidinyl, morpholinyl, piperazinyl, piperidinyl, py razol idinyl, pyrrolidinyl, tetrahydrofuranyl, 15 tetrahydropyranyl, tetrahydrothiopyranyl, thiazanyl, and the like. A cyclic group may be bonded to another group in more than one way. If no particular bonding arrangement is specified, then all possible arrangements are intended. For example, the term "pyridyl" includes 2-, 3- or 4-pyridyl (2-, 3-, or 4-pyridinyl). 20 The term "mammal" means animals including, for example, dogs, cats, cows, sheep, goats, horses and humans. Preferred mammals include humans. The term "oxo" means a carbonyl group formed by the combination of a carbon atom and an oxygen atom. 25 The term "patient" includes both human and non-human patients. The phrase "pharmaceutically acceptable" indicates that the designated carrier, vehicle, diluent, and/or salt is generally chemically and/or physically compatible with the other ingredients comprising the formulation, and physiologically compatible with the recipient thereof. 30 The term "salts" refers to both organic and inorganic salts of a compound of Formula (I). Such salts can be prepared in situ during the final WO 2008/139293 PCT/IB2008/001125 -26- isolation and purification of a compound, or by separately reacting a compound, prodrug or stereoisomer of Formula (I) with a suitable organic or inorganic acid or base and isolating the salt thus formed. Representative anionic salts include hydrobromide, hydrochloride, hydroiodide, sulfate, 5 bisulfate, nitrate, acetate, trifluoroacetate, oxalate, besylate, palmitate, pamoate, malonate, stearate, laurate, malate, borate, benzoate, lactate, phosphate, hexafiuorophosphate, benzene sulfonate, tosylate, formate, citrate, maleate, fumarate, succinate, tartrate, naphthylate, mesylate, glucoheptonate, lactobionate and laurylsulphonate salts and the like. 10 Representative cationic salts include sodium, potassium, calcium, and magnesium salts and the like. See generally, e.g., Berge, et al., J. Pharm. Set., 66, 1-19 (1977). A salt of a compound of Formula (I) may be readily prepared by mixing together solutions of a compound of Formula (I) and the desired acid or base, 15 as appropriate. The salt may precipitate from solution and be collected by filtration or may be recovered by evaporation of the solvent. The term "radical" denotes a group of atoms that behaves as a single reactant in a chemical reaction, e. g., an organic radical is a group of atoms that imparts characteristic properties to a compound containing it, or which 20 remains unchanged during a series of reactions or transformations. The symbol" -" represents a covalent bond. The phrase "reaction-inert solvent" or "inert solvent" refers to a solvent, or mixture of solvents, that does not interact with starting materials, reagents, intermediates or products in a manner that adversely affects their desired 25 properties. The terms "treat," "treating," "treated" or "treatment" as used herein includes preventative (e.g., prophylactic), palliative or curative uses or results. The compounds of Formula (I) may contain asymmetric or chiral centers and, therefore, exist in different stereoisomeric forms. Those skilled in 30 the art will appreciate that, unless otherwise specified, all stereoisomers (e.g., enantiomers and diastereoisomers, and racemic mixtures thereof) of the novel WO 2008/139293 PCT/IB2008/001125 -27- compounds and intermediates described, illustrated and/or discussed herein are within the scope of the claimed invention. In addition, unless otherwise specified, the present invention embraces all geometric and positional isomers. The (3S.4S) enantiomer of the core pyrrolidinyl configuration is 5 preferred. Diasteriomeric mixtures can be separated into their individual diastereomers on the basis of their physical chemical differences by methods well-known to those of ordinary skill in the art, such as by chromatography and/or fractional crystallization. Enantiomers can be separated by converting 10 the enantiomeric mixture into a diasteriomeric mixture by reaction with an appropriate optically active compound (e.g., alcohol), separating the diastereomers and converting (e.g., hydrolyzing) the individual diastereomers to the corresponding pure enantiomers. Additional methods include resolution of racemic mixtures using chiral salts, as well as chiral chromatography. 15 Those skilled in the art will further recognize that the compounds of Formula (I) can exist in crystalline form as hydrates wherein molecules of water are incorporated within the crystal structure thereof and as solvates wherein molecules of a solvent are incorporated therein. All such hydrate and solvate forms are considered part of this invention. 20 Practitioners will appreciate that certain compounds of Formula (I) may exist as tautomeric isomers, i.e., that equilibrium exists between two isomers which are in rapid equilibrium with each other. A common example of tautomerism is keto-enol tautomerism, i.e., 25 The degree to which one tautomer is present over the other depends upon various factors, including substitution pattern and solvent type. Other examples in accordance with the present invention will be recognized by those skilled in the art. All tautomeric forms of Formula (I) are included within the scope of the invention unless otherwise specified. WO 2008/139293 PCT/IB2008/001125 -28- The present invention also includes prodrugs of the compounds of the invention. The term "prodrug" refers to a drug precursor which, following administration, releases the drug in vivo via a chemical or physiological process (e.g., upon being brought to physiological pH or through enzymatic 5 activity). The prodrug may itself be biologically active, or may be converted to a biologically active compound (e.g. by metabolism or hydrolysis) during its residence time in the body. A discussion of the preparation and use of prodrugs is provided by Higuchi & Stella, "Prodrugs as Novel Delivery Systems", Vol.14 of the A.C.S. Symposium Series, and in "Bioreversible 10 Carriers in Drug Design," ed. Edward B. Roche, American Pharmaceutical Association and Pergamon Press, 1987. All prodrugs of the various compounds of Formula (I) are within the scope of the present invention. The present invention also embraces isotopically-labeled compounds of Formula (I) that are identical to those recited herein, but for the fact that 15 one or more atoms are replaced by an atom having an atomic mass or mass number different from the atomic mass or mass number usually found in nature. Examples of isotopes that can be incorporated into compounds of Formula (I) include isotopes of hydrogen, carbon, nitrogen, oxygen, phosphorus, fluorine, and chlorine, such as 2H, 3H, 13C, 14C, 15N, 180, 170,31P, 20 32P, 35S, 18F and 36CI, respectively. The compounds of Formula (I), and pharmaceutically acceptable salts thereof, that contain the aforementioned isotopes and/or other isotopes of the other atoms are within the scope of the instant invention. Certain isotopically-labeled compounds of Formula (I), for example 25 those into which radioactive isotopes such as 3H and 14C are incorporated, are useful in drug and/or substrate tissue distribution assays. Tritiated, i.e., 3H and 14C, isotopes are particularly preferred for their ease of preparation and detectability. Furthermore, substitution with heavier isotopes such as deuterium, i.e., 2H, may afford certain therapeutic advantages resulting from 30 greater metabolic stability, for example, increased in vivo half-life, or reduced dosage requirements and, hence, may be preferred in some circumstances. WO 2008/139293 PCT/IB2008/001125 -29- Isotopically-labeled compounds of Formula (I), and pharmaceutically acceptable salts thereof, can be generally prepared by carrying out analogous procedures to those disclosed in the Schemes and/or in the Examples below, by substituting a readily available isotopically-labeled reagent for a non-5 isotopically labeled reagent. The invention also includes pharmaceutical compositions comprising an amount of a compound of Formula (I), or a pharmaceutically acceptable salt of the compound, and optionally a pharmaceutically acceptable vehicle, carrier or diluent. In a preferred embodiment, the pharmaceutical composition 10 is of an amount effective at inhibiting the enzyme PDE9 in a mammal. In another preferred embodiment, the mammal is a human. The present invention includes the use of a combination of a PDE9 inhibitor compound as provided in Formula (I) and one or more additional pharmaceutically active agent(s). If a combination of active agents is 15 administered, then they may be administered sequentially or simultaneously, in separate dosage forms or combined in a single dosage form. Accordingly, the present invention also includes pharmaceutical compositions comprising an amount of: (a) a first agent comprising a compound of Formula (I) or a pharmaceutically acceptable salt of the compound; (b) a second 20 pharmaceutically active agent; and (c) a pharmaceutically acceptable carrier, vehicle or diluent. Various pharmaceutically active agents may be selected for use in conjunction with the compounds of Formula (I), depending on the disease, disorder, or condition to be treated. Pharmaceutically active agents that may 25 be used in combination with the compositions of the present invention include, without limitation: (i) acetylcholinesterase inhibitors, such as donepezil hydrochloride (E2020, ARICEPT, MEMAC), physostigmine salicylate (ANTILIRIUM), physostigmine sulfate (ESERINE), metrifonate, neostigmine, pyridostigmine 30 (MESTINON), ambenonium (MYTELASE), demarcarium, Debio992 (also known as ZT-1), rivastigmine (EXELON), ladostigil (also known as TV3326), WO 2008/139293 PCT/IB2008/001125 -30- NP-0361, galantamine hydrobromide (RAZADYNE, RIMINYL, NIVALIN), tacrine (COGNEX), veinacrine maleate, memoquin, huperzine A (HUP-A), phenserine, and edrophonium (ENLON, TENSILON); (ii) amyloid-IJ (or fragments thereof), such as AGms conjugated to pan 5 HLA DR-binding epitope (PADRE), ACC-001, ACI-01, ACI-24, AN-1792, Affitope AD-01, CAD106, and V-950; (iii) antibodies to amyloid-H (or fragments thereof), such as bapineuzumab (also known as AAB-001), AAB-002, ACI-01-Ab7, BAN-2401, intravenous Ig (GAMMAGARD), LY2062430 (humanized m266), PF- 10 04360365 (also known as RN-1219), RN-6G, R-1450, ACU-5A5, huC091, and those disclosed in International Patent Publication Nos W004/032868, W005/025616, W006/036291, W006/069081, W006/118959, in US Patent Publication Nos US2003/0073655, US2004/0192898, US2005/0048049, US2005/0019328, in European Patent Publication Nos EP0994728 and 15 1257584, and in US Patent No 5,750,349; (iv) amyloid-lowering or -inhibiting agents (including those that reduce amyloid accumulation and fibrillization) such as bisnorcymserine (also known as BNC), pioglitazone, PBT2, flurbiprofen (ANSAID, FROBEN) and its R-enantiomer tarenflurbil (also known as MPC-7869; FLURIZAN), 20 nitroflurbiprofen, fenoprofen (FENOPRON, NALFON), ibuprofen (ADVIL, MOTRIN, NUROFEN), ibuprofen lysinate, meclofenamic acid, meclofenamate sodium (MECLOMEN), indomethacin (INDOCIN), diclofenac sodium (VOLTAREN), diclofenac potassium, sulindac (CLINORIL), sulindac sulfide, diflunisal (DOLOBID), naproxen (NAPROSYN), naproxen sodium 25 (ANAPROX, ALEVE), insulin-degrading enzyme (also known as insulysin), the gingko biloba extract EGb-761 (ROKAN, TEBONIN), tramiprosate (NC-758, CEREBRIL, ALZHEMED), eprodisate (NC-503, FIBRILLEX, KIACTA), compound W (3,5-bis(4-nitrophenoxy)benzoic acid), NGX-96992, neprilysin (also known as neutral endopeptidase (NEP)), scyllo-inositol (also known as 30 scyllitol, ELND005, AZD-103), atorvastatin (LIPITOR), simvastatin (ZOCOR), WO 2008/139293 PCT/IB2008/001125 -31- KLVFF-(EEX)3, and RAGE (receptor for advanced glycation end-products) inhibitors; (v) alpha-adrenergic receptor agonists, such as clonidine (CATAPRES), metaraminol (ARAMINE), methyldopa (ALDOMET, DOPAMET, 5 NOVOMEDOPA), tizanidine (ZANAFLEX), phenylephrine (also known as neosynephrine), methoxamine, cirazoline, guanfacine (INTUNIV), lofexidine, xylazine, modafinil (PROVIGIL), adrafinii, and armodafinil (NUVIGIL); (vi) beta-adrenergic receptor blocking agents (beta blockers), such as carteolol, esmolol (BREVIBLOC), labetalol (NORMODYNE, TFIANDATE), 10 oxprenolol (LARACOR, TRASACOR), pindolol (VISKEN), propanolol (INDERAL), sotalol (BETAPACE, SOTALEX, SOTACOR), timolol (BLOGADREN, TIMOPTIC), acebutolol (SECTRAL, PRENT), nadolol (CORGARD), metoprolol tartrate (LOPRESSOR), metoprolol succinate (TOPROL-XL), atenolol (TENORMIN), butoxamine, and SR 59230A (Sanofi); 15 (vii) anticholinergics, such as amitriptyline (ELAVIL, ENDEP), butriptyline, benztropine mesylate (COGENTIN), trihexyphenidyl (ARTANE), diphenhydramine (BENADRYL), orphenadrine (NORFLEX), hyoscyamine, atropine (ATROPEN), scopolamine (TRANSDERM-SCOP), scopolamine methylbromide (PARMINE), dicycloverine (BENTYL, BYCLOMINE, DIBENT, 20 DILOMINE, tolterodine (DETROL), oxybutynin (DITROPAN, LYRINEL XL, OXYTROL), penthienate bromide, propantheline (PRO-BANTHINE), cyclizine, imipramine hydrochloride (TOFRANIL), imipramine maleate (SURMONTIL), lofepramine, desipramine (NORPRAMIN), doxepin (SINEQUAN, ZONALON), trimipramine (SURMONTIL), and glycopyrrolate (ROBINUL); 25 (viii) anticonvulsants, such as carbamazepine (TEGRETOL, CARBATROL), oxcarbazepine (TRILEPTAL), phenytoin sodium (PHENYTEK), fosphenytoin (CEREBYX, PRODILANTIN), divalproex sodium (DEPAKOTE), gabapentin (NEURONTIN), pregabalin (LYRICA), topirimate (TOPAMAX), valproic acid (DEPAKENE), valproate sodium (DEPACON), 1-30 benzyl-5-bromouracil, progabide, beclamide, and primidone (MYSOLINE); WO 2008/139293 PCT/IB2008/001125 -32- (ix) antipsychotics, such as lurasidone (also known as SM-13496), aripiprazole (ABILIFY), chlorpromazine (THOfRAZINE), haloperidol (HALDOL), flupentixoi decanoate (DEPIXOL, FLUANXOL), reserpine (SERPLAN), pimozide (ORAP), fluphenazine decanoate, fluphenazine 5 hydrochloride, prochlorperazine (COMPRO), asenapine, loxapine (LOXITANE), mesoridazine, molindone (MOBAN), perphenazine, thioridazine, thiothixine, trifluoperazine (STELAZINE), clozapine (CLOZARIL), norclozapine (ACP-104), risperidone (RISPERDAL), paliperidone (INVEGA), melperone, olanzapine (ZYPREXA), quetiapine (SEROQUEL), sertindole, sulpiride 10 (MERESA, DOGMATYL, SULPITIL), amisulpride, ziprasidone (GEODON), blonanserin (LONASEN), and bifeprunox; (x) calcium channel blockers such as nilvadipine (ESCOR, NIVADIL), amlodipine (NORVASC, ISTIN, AMLODIN), felodipine (PLENDIL), nicardipine (CARDENE), nifedipine (ADALAT, PROCARDIA), MEM 1003 and its parent 15 compound nimodipine (NIMOTOP), nisoldipine (SULAR), nitrendipine, lacidipine (LACIPIL, MOTENS), lercanidipine (ZANIDIP), diltiazem (CARDIZEM), verapamil (CALAN, VERELAN), and enecadin (also known as NS-7); (xi) catechol O-methyltransferase (COMT) inhibitors, such as 20 tolcapone (TASMAR), entacapone (COMTAN), and tropolone; (xii) central nervous system stimulants, such as caffeine, phenmetrazine, phendimetrazine, pemoline, fencamfamine (GLUCOENERGAN, REACTIVAN), fenethylline (CAPTAGON), pipradol (MERETRAN), deanol (also known as dimethylaminoethanol), 25 methylphenidate (DAYTRANA), methylphenidate hydrochloride (RITALIN), dexmethylphenidate (FOCALIN), amphetamine (alone or in combination with other CNS stimulants, e.g. ADDERALL (amphetamine aspartate, amphetamine sulfate, dextroamphetamine saccharate, and dextroamphetamine sulfate)), dextroamphetamine sulfate (DEXEDRINE, 30 DEXTROSTAT), methamphetamine (DESOXYN), lisdexamfetamine (VYVANSE), and benzphetamine (DIDREX); WO 2008/139293 PCT/IB2008/001125 (xiii) corticosteroids, such as prednisone (STERAPRED, DELTASONE), prednisolone (PRELONE), predisolone acetate (OMNIPRED, PRED MILD, PRED FORTE), prednisolone sodum phosphate (OFRAPRED ODT), methylprednisolone (MEDROL); methylprednisolone acetate (DEPO- 5 MEDROL), and methylprednisolone sodium succinate (A-METHAPRED, SOLU-MEDROL); (xiv) dopamine receptor agonists, such as apomorphine (APOKYN), bromocriptine (PARLODEL), cabergoline (DOSTINEX), dihydrexidine, dihydroergocryptine, fenoldopam (CORLOPAM), lisuride (DOPERGIN), 10 pergolide (PERMAX), piribedil (TRIVASTAL, TRASTAL), pramipexole (MIRAPEX), quinpirole, ropinirole (REQUIP), and rotigotine (NEUPRO); (xv) dopamine receptor antagonists, such as tetrabenazine (NITOMAN, XENA2INE), 7-hydroxyamoxapine, droperidol (INAPSINE, DRIDOL, DROPLETAN), domperidone (MOTILIUM), L-741742, L-745870, 15 raclopride, SCH-23390, ecopipam, SKF-83566, and metoclopramide (REGLAN); (xvi) dopamine reuptake inhibitors such as nomifensine maleate (MERITAL), vanoxerine (also known as GBR-12909) and its decanoate ester DBL-583, and amineptine; 20 (xvii) gamma-amino-butyric acid (GABA) receptor agonists, such as baclofen (LIORESAL, KEMSTRO), pentobarbital (NEMBUTAL), progabide (GABRENE), and clomethiazole; (xviii) immunomodulators such as glatiramer acetate (also known as copolymer-1; COPAXONE), MBP-8298 (synthetic myelin basic protein 25 peptide), dimethyl fumarate, fingolimod (also known as FTY720), roquinimex (LINOMIDE), laquinimod (also known as ABR-215062 and SAIK-MS), ABT-874 (human anti-IL-12 antibody), rituximab (RITUXAN), alemtuzumab (CAMPATH), daclizumab (ZENAPAX), and natalizumab (TYSABRI); (xix) immunosuppressants such as methotrexate (TREXALL, 30 RHEUMATREX), mitoxantrone (NOVANTRONE), mycophenolate mofetil (CELLCEPT), mycophenolate sodium (MYFORTIC), azathioprine (AZASAN, WO 2008/139293 PCT/IB2008/001125 -34- IMURAN), mercaptopurine (PURI-NETHOL), cyclophosphamide (NEOSAR, CYTOXAN), chlorambucil (LEUKERAN), cladribine (LEUSTATIN, MYLINAX), alpha-fetoprotein, etanercept (ENBREL), and 4-benzyioxy-5-((5-undecyl-2H-pyrrol-2-ylidene)methyl)-2,2'-bi-1 H-pyrrole (also known as PNU-156804); 5 (xx) interferons, including interferon beta-1a (AVONEX, REBIF) and interferon beta-1b (BETASERON, BETAFERON); (xxi) levodopa (or its methyl or ethyl ester), alone or in combination with a DOPA decarboxylase inhibitor (e.g. carbidopa (SINEMET, CARBILEV, PARCOPA, V1512), benserazide (MADOPAR), a-methyldopa, 10 monofluromethyldopa, difluoromethyldopa, brocresine, or m-hydroxybenzylhydrazine); (xxii) /V-methyl-D-aspartate (NMDA) receptor antagonists, such as memantine (NAMENDA, AXURA, EBIXA), amantadine (SYMMETREL), acamprosate (CAMPRAL), besonprodil (also known as PD-196,860 or Cl- 15 1041), ketamine (KETALAR), delucemine (also known as NPS 1506), dexanabinol (also known as HU-211), dextromethorphan, dextrorphan, traxoprodil (also known as CP-101,606), himantane, idantadol (also known as V-3381), lancicemine (also known as AR-R 15896), levorphanol (DROMORAN), methadone, (DOLOPHINE), neramexane (also known as 20 MRZ 2/579), perzinfotel, phencyclidine, tianeptine (STABLON), dizocilpine (also known as MK-801), ibogaine, voacangine, tiletamine, riluzole (RILUTEK), aptiganel (CERESTAT), gavestinel, and remacimide; (xxiii) monoamine oxidase (MAO) inhibitors, such as selegiline (EMSAM), selegiline hydrochloride (l-deprenyl, ELDEPRYL, ZELAPAR), 25 dimethylselegilene, brofaromine, phenelzine (NARDIL), tranylcypromine (PARNATE), moclobemide (AURORIX, MANERIX), befloxatone, safinamide (also known as PNU-151774E), isocarboxazid (MARPLAN), nialamide (NIAMID), rasageline (AZILECT), iproniazide (MARSILID, IPROZID, IPRONID), iproclozide, toloxatone (HUMORYL, PERENUM), bifemelane, 30 desoxypeganine, harmine (also known as telepathine or banasterine), WO 2008/139293 PCT/IB2008/001125 -35- harmaline, linezolid (ZYVOX, ZYVOXID), and pargyline (EUDATIN, SUPIRDYL); (xxiv) muscarinic receptor (particularly M1 subtype) agonists, such as bethanechol chloride (DUVOID, URECHOLINE), pilocarpine (SALAGEN), 5 NGX267, arecoline, L-687306, L-689660, furtrethonium iodide (FURAMON, FURANOL), furtrethonium benzensulfonate, furtrethonium p-toluenesulfonate, McN-A-343, oxotremorine, and carbachol (CARBASTAT, MIOSTAT, CARBOPTIC); (xxv) nicotinic receptor agonists, such as epibatidine, ABT-089, ABT-10 594, AZD-0328, R-4996 (also known as MEM-63908), TC-5619, and EVP- 6124; (xxvi) neuroprotective drugs such as 2,3,4,9-tetrahydro-1 H-carbazol-3-one oxime, AL-108, ACD3480 (also known as TC-1734), bis(4-(3-D-glucopyranosyioxybenzyl)-2-(3-D-glucopyranosyl-2-isobutyltartrate (also 15 known as dactyiorhin B or DHB), xaliproden (XAPRILA), dimeboline hydrochloride (DIMEBON), disufenton (NXY-059, CEROVIVE), arundic acid (ON0-2506, PROGLIA, CEREACT), citicoline (also known as cytidine 5'-diphosphocholine), edaravone (RADICUT), AEOL-10150, AGY-94806 (also known as SA-450 and Msc-1), granulocyte-colony stimulating factor (AX-200), 20 BAY-387271 (also known as KN-387271), DP-b99, HF-0220 (17-fi-hydroxyepiandrosterone), HF-0420 (also known as oligotropin), pyridoxal 5'-phosphate (also known as MC-1), microplasmin, S-18986, piclozotan (also known as SUN-N4057), NP031112, L-seryl-L-methionyl-L-alanyl-L-lysyl-L-glutamyl-glycyl-L-valine, and SUN-N8075; 25 (xxvii) norepinephrine (noradrenaline) reuptake inhibitors, such as atomoxetine (STRATTERA), doxepin (APONAL, ADAPIN, SINEQUAN), nortriptyline (AVENTYL, PAMELOR, NORTRILEN), amoxapine (ASENDIN, DEMOLOX, MOXIDIL), reboxetine (EDRONAX, VESTRA), viloxazine (VIVALAN), maprotiline (DEPRILEPT, LUDIOMIL, PSYMION), bupropion 30 (WELLBUTRIN), and radaxafine; WO 2008/139293 PCT/IB2008/001125 -36- (xxviii) other PDE9 inhibitors, such as BAY 73-6691 and those disclosed in US Patent Publication Nos US2003/0195205, US2004/0220186, US2006/0111372, and US2006/0106035; (xxix) other phosphodiesterase (PDE) inhibitors, including (a) PDE1 5 inhibitors (e.g. vinpocetine (CAVINTON, CERACTIN, INTELECTOL) and those disclosed in US Patent No 6,235,742), (b) PDE2 inhibitors (e.g. erythro-9-(2-hydroxy-3-nonyl)adenine (EHNA), BAY 60-7550, and those described in US Patent No. 6,174,884), (c) PDE4 inhibitors (e.g. rolipram, Ro 20-1724, ibudilast (KETAS), piclamilast (also known as RP73401), CDP840, cilomilast 10 (ARIFLO), roflumilast, tofimilast, oglemilast (also known as GRC 3886), tetomilast (also known as OPC-6535), lirimifast, theophylline (UNIPHYL, THEOLAIR), arofylline (also known as LAS-31025), doxofylline, RPR-122818, or mesembrine), and (d) PDE5 inhibitors (e.g. sildenafil (VIAGRA, REVATIO), tadalafil (CIALIS), vardenafil (LEVITF^A, VIVANZA), udenafil, avanafil, 15 dipyridamole (PERSANTINE), E-4010, E-4021, E-8010, zaprinast, PF-489791, UK-357903, DA-8159, and those disclosed in International Patent Applications W005/049616, W006/120552, and W007/122466); (xxx) quinolines, such as quinine (including its hydrochloride, dihydrochloride, sulfate, bisulfate and gluconate salts), chloroquine, 20 hydroxychloroquine (PLAQUENIL), mefloquine (LARIAM), and amodiaquine (CAMOQUIN, FLAVOQUINE); (xxxi) p-secretase inhibitors, such as WY-25105, (+)-phenserine tartrate (POSIPHEN), LSN-2434074 (also known as LY-2434074), PNU-33312, KMI-574, SCH-745966, Ac-rER (N2-acetyl-D-arginyl-L-arginine), 25 loxistatin (also known as E64d), and CA074Me; (xxxii) y-secretase inhibitors, such as LY-411,575, LY-685,458, ELAN-G, ELAN-Z, 4-chloro-A/-[2-ethyl-1 (S)-(hydroxymethyl)butyl]benzenesulfonamide, (xxxiii) serotonin (5-hydroxytryptamine) 1A (5-HTiA) receptor 30 antagonists, such as spiperone, /ei/o-pindolol, BMY 7378, NAD-299, S(-)-UH- 301, NAN 190, WAY 100635, lecozotan (also known as SRA-333); WO 2008/139293 PCT/IB2008/001125 -37- (xxxiv) serotonin (5-hydroxytryptamine) 6 (5-HTe) receptor antagonists, such as mianserin (TORVOL, BOLVIDON, NORVAL), methiothepin (also known as metitepine), ritanserin, ALX-1161, ALX-1175, MS-245, LY-483518 (also known as SGS518), MS-245, Ro 04-6790, RO 43-5 68544, Ro 63-0563, RO 65-7199, Ro 65-7674, SB-399885, SB-214111, SB-258510, SB-271046, SB-357134, SB-699929, SB-271046, SB-742457 and PRX-07034; (xxxv) serotonin (5-HT) reuptake inhibitors such as alaproclate, citalopram (CELEXA, CIPRAMIL), escitalopram (LEXAPRO, CIPRALEX), 10 clomipramine (ANAFRANIL), duloxetine (CYMBALTA), femoxetine (MALEXIL), fenfluramine (PONDIMIN), norfenfluramine, fluoxetine (PROZAC), fluvoxamine (LUVOX), indalpine, milnacipran (IXEL), paroxetine (PAXIL, SEROXAT), sertraline (ZOLOFT, LUSTRAL), trazodone (DESYREL, MOLIPAXIN), venlafaxine (EFFEXOR), zimelidine (NORMUD, ZELMID), 15 bicifadine, desvenlafaxine (PRISTIQ), brasofensine, and tesofensine; (xxxvi) trophic factors, such as nerve growth factor (NGF), basic fibroblast growth factor (bFGF), neurotrophin-3 (NT-3), brain-derived neurotrophic factor (BDNF), and glial-derived neurotrophic factor (GDNF), and agents that stimulate local production of trophic factors, such as 20 propentofylline, idebenone, and AIT-082 (NEOTROFIN); and the like. The invention also includes methods of inhibiting PDE9 in a mammal comprising administering to the mammal in need of such inhibition a PDE9 inhibiting amount of: (a) a compound of Formula (I), or a pharmaceutically 25 acceptable salt thereof; or (b) a pharmaceutical composition comprising a compound of Formula (I), or a pharmaceutically acceptable salt thereof, in a pharmaceutically acceptable vehicle, carrier or diluent; either alone or in combination with a second agent as described above. The invention also includes methods of treating conditions mediated by 30 PDE9 inhibition in a mammal comprising administering to the mammal in need of such treatment a therapeutically effective amount of: (a) a compound of WO 2008/139293 PCT/IB2008/001125 -38- Formula (I), or a pharmaceutically acceptable salt thereof; or (b) a pharmaceutical composition comprising a compound of Formula (I), or a pharmaceutically acceptable salt thereof, in a pharmaceutically acceptable vehicle, carrier or diluent; either alone or in combination with a second agent 5 described above. Conditions that may be treated, controlled or prevented by the methods of the present invention include diseases and disorders associated with neurodegeneration such as: Alexander disease, Alper's disease, Alzheimer's disease, amyotrophic lateral sclerosis (ALS; also known as Lou Gehrig's 10 disease or motor neuron disease), ataxia-telangiectasia, Batten disease (also known as Spielmeyer-Vogt-Sjogren-Batten disease), Binswanger"s dementia (subcortical arteriosclerotic encephalopathy), bipolar disorders, bovine spongiform encephalopathy (BSE), Canavan disease, chemotherapy-induced dementia, Cockayne syndrome, corticobasal degeneration, Creutzfeldt-Jakob 15 disease, depression, Down syndrome, frontotemporal lobar degeneration (including frontotemporal dementia, semantic dementia, and progressive nonfluent aphasia), Gerstmann-Straussler-Scheinker disease, glaucoma, Huntington's disease (chorea), HIV-associated dementia, hyperkinesias, Kennedy's disease, Korsakoff's syndrome (amnesic-confabulatory syndrome), 20 Krabbe's disease, Lewy body dementia, logopenic progressive aphasia, Machado-Joseph disease (spinocerebellar ataxia type 3), multiple sclerosis, multiple system atrophy (olivopontocerebellar atrophy), myasthenia gravis, Parkinson's disease, Pelizaeus-Merzbacher disease, Pick's disease, presenile dementia (mild cognitive impairment), primary lateral sclerosis, primary 25 progressive aphasia, radiation-induced dementia, Refsum's disease (phytanic acid storage disease), Sandhoff disease, Schilder's disease, schizophrenia, semantic dementia, senile dementia, Shy-Drager syndrome, spinocerebellar ataxias, spinal muscular atrophies, Steele-Richardson-Olszewski disease (progressive supranuclear palsy), tabes dorsalis, tardive dyskinesia, vascular 30 amyloidosis, and vascular dementia (multi-infarct dementia). WO 2008/139293 PCT/IB2008/001125 -39- Preferably the neurodegenerative disease or disorder is Alzheimer's disease. Other conditions and disorders associated with PDE9 that may be treated or controlled by the methods of the present invention include disorders 5 of the urogenital system such as sexual dysfunction, attention deficit disorder (ADD), attention deficit hyperactivity disorder (ADHD), diabetes, cardiovascular disorders or diseases such as systemic hypertension, pulmonary hypertension, congestive heart failure, coronary artery disease, atherosclerosis, stroke, thrombosis, conditions of reduced blood vessel 10 patency (e.g. post-percutaneous transluminal coronary angioplasty), peripheral vascular disease, renal disease, angina (including stable, unstable, and variant (Prinzmetal) angina), and any condition where improved blood flow leads to improved end organ function. The present invention also relates to methods for promoting 15 neurorestoration and functional recovery in patients suffering from traumatic or non-traumatic injury to the brain, spinal cord or peripheral nerves. Traumatic brain injuries include both closed head injuries (in which the skull is not broken) and open, or penetrating, head injuries (in which an object pierces the skull and breaches the dura mater), wherein sudden trauma (e.g., 20 accidents, falls, assaults) causes damage to the brain tissue by tearing, stretching, bruising, or swelling. Causes of non-traumatic brain injuries include aneurism, stroke, meningitis, oxygen deprivation due to anoxia, hypoxia, or ischemia, brain tumor, infection (e.g. encephalitis), poisoning, substance abuse, and the like. The present invention is useful for the 25 treatment of cognitive impairment and cognitive dysfunction resulting from brain injuries as well as from neurodegenerative diseases and disorders. The present invention also relates to methods for preventing the above-described conditions in a mammal, including human, comprising the steps of administering to the mammal an amount of: (a) a compound of Formula (I), or 30 a pharmaceutically acceptable salt thereof; or (b) a pharmaceutical composition comprising a compound of Formula (I), or a pharmaceutically WO 2008/139293 PCT/IB2008/001125 -40- acceptable salt thereof, in a pharmaceutically acceptable vehicle, carrier or diluent; either alone or in combination with a second agent as described above, as part of an appropriate dosage regimen designed to prevent said condition. 5 The present invention also relates to methods for improving cognitive deficits, including deficits in perception, concentration, learning, memory, communication, reasoning, and problem-solving. The appropriate dosage regimen, the amount of each dose administered and the intervals between doses of the compound will depend, 10 among others, upon the compound of Formula (I) of this invention being used, the type of pharmaceutical compositions being used, the characteristics of the subject being treated and the type and severity of the conditions to be treated. In general, an effective dose for compounds of Formula (I) or pharmaceutically acceptable salts thereof, is in the range of from about 0.1 15 mg to about 3,500 mg per day. For a normal adult human having a body mass of about 70 kg, a dosage in the range of from about 0.01 mg to about 50 mg per kg body mass is typically sufficient, and preferably from about 0.2 to 2.5 mg per kg, in single or divided doses daily. Administration may be in single (e.g., once daily) or multiple doses or via constant infusion. 20 Some variability in the general dosage range may be required depending upon the age and mass of the subject being treated, the intended route of administration, the particular compound being administered, and the like. The determination of dosage ranges and optimal dosages for a particular mammalian subject is within the ability of a skilled person having benefit of the 25 instant disclosure. The compounds of Formula (I) may be administered by a variety of conventional routes of administration, including oral, buccal, sublingual, ocular, topical (e.g., transdermal), parenteral (e.g., intravenous, intramuscular, or subcutaneous), rectal, intracisternal, intravaginal, intraperitoneal, 30 intravesical, local (e.g., powder, ointment, or drop), nasal and/or inhalation dosage forms or using a "flash" formulation, i.e., allowing the medication to WO 2008/139293 PCT/IB2008/001125 -41- dissolve in the mouth without the need to use water. As will be recognized by one of skill in the art, the appropriate dosage regimen, the amount of each dose administered and the intervals between doses of the compound will depend upon the compound of Formula (I), or the prodrug thereof, being 5 used, the type of pharmaceutical compositions being used, the characteristics of the subject being treated, and/or the severity of the conditions being treated. Methods of preparing various pharmaceutical compositions with amounts of active ingredients are known, or will be apparent in light of this 10 disclosure, to those skilled in this art. See, for example, Remington's Pharmaceutical Sciences, Mack Publishing Co., Easton, PA, 19th Ed. (1995). Suitable pharmaceutical carriers, vehicles and diluents for such compositions include inert solid diluents or fillers, sterile aqueous solutions and various organic solvents. The pharmaceutical compositions formed by 15 combining a compound of this invention and pharmaceutically acceptable carriers, vehicles or diluents are readily administered in a variety of dosage forms such as tablets, powders, lozenges, syrups, injectable solutions and the like. Solid dosage forms for oral administration include capsules, tablets, 20 powders, and granules. In such solid dosage forms, the active compound is admixed with at least one inert conventional pharmaceutical excipient (or carrier) such as sodium citrate, calcium carbonate, or dicalcium phosphate, or (a) fillers or extenders, such as for example, starches, lactose, sucrose, mannitol and silicic acid; (b) binders, such as for example, 25 carboxymethylcellulose, alginates, gelatin, polyvinylpyrrolidone, sucrose and acacia; (c) humectants, such as for example, glycerol; (d) disintegrating agents, such as for example, agar-agar, calcium carbonate, potato or tapioca starch, alginic acid, certain complex silicates, and sodium carbonate; (e) solution retarders, such as for example, paraffin; (f) absorption accelerators, 30 such as for example, quaternary ammonium compounds; (g) wetting agents, such as for example, cetyl alcohol and glycerol monostearate; (h) adsorbents, WO 2008/139293 PCT/IB2008/001125 -42- such as for example, kaolin and bentonite; and/or (i) lubricants, such as for example, talc, calcium stearate, magnesium stearate, solid polyethylene glycols, sodium lauryl sulfate or mixtures thereof. In the case of capsules and tablets, the dosage forms may further comprise buffering agents. 5 Solid dosage forms may be formulated as modified release and pulsatile release dosage forms containing excipients such as those detailed above for immediate release dosage forms together with additional excipients that act as release rate modifiers, these being coated on and/or included in the body of the device. Release rate modifiers include, but are not limited to, 10 hydroxypropylmethyl cellulose, methyl cellulose, sodium carboxymethylcellulose, ethyl cellulose, cellulose acetate, polyethylene oxide, xanthan gum, ammoniomethacrylate copolymer, hydrogenated castor oil, carnauba wax, paraffin wax, cellulose acetate phthalate, hydroxypropylmethyl cellulose phthalate, methacrylic acid copolymer and mixtures thereof. 15 Modified release and pulsatile release dosage forms may contain one or a combination of release rate modifying excipients. The pharmaceutical compositions of the invention may further comprise fast dispersing or dissolving dosage formulations (FDDFs). The terms dispersing or dissolving as used herein to describe FDDFs are 20 dependent upon the solubility of the drug substance used i.e., where the drug substance is insoluble, a fast dispersing dosage form may be prepared, and where the drug substance is soluble, a fast dissolving dosage form may be prepared. Solid compositions of a similar type may also be employed as fillers in 25 soft or hard filled gelatin capsules using such excipients as lactose or milk sugar, as well as high molecular weight polyethylene glycols and the like. Solid dosage forms such as tablets, dragees, capsules, and granules can be prepared with coatings and shells, such as enteric coatings and others well-known to one of ordinary skill in the art. They may also comprise 30 opacifying agents, and can also be of such composition that they release the active compound(s) in a delayed, sustained or controlled manner. Examples WO 2008/139293 PCT/IB2008/001125 -43- of embedding compositions that can be employed are polymeric substances and waxes. The active compound(s) can also be in micro-encapsulated form, if appropriate, with one or more of the above-mentioned excipients. Liquid dosage forms for oral administration include pharmaceutically 5 acceptable emulsions, solutions, suspensions, syrups, and elixirs. In addition to the active compounds, the liquid dosage form may contain inert diluents commonly used in the art, such as water or other solvents, solubilizing agents and emulsifiers, as for example, ethanol, isopropanol, ethyl carbonate, benzyl benzoate, propylene glycol, 1,3-butylene glycol, oils (in particular, cottonseed 10 oil, groundnut oil, corn germ oil, olive oil, castor oil, and sesame seed oil), glycerol, tetrahydrofurfuryl alcohol, polyethylene glycols and fatty acid esters of sorbitan, or mixtures of these substances, and the like. In addition to the active compound(s), the pharmaceutical composition may further include suspending agents, such as for example, ethoxylated 15 isostearyl alcohols, polyoxyethylene sorbitol and sorbitan esters, microcrystalline cellulose, aluminum metahydroxide, bentonite, agar-agar, and tragacanth, or mixtures of these substances, and the like. Sweeteners, flavoring, and perfuming agents may also be included. The pharmaceutical compositions of the invention may further comprise 20 adjuvants, such as preserving, wetting, emulsifying and dispersing agents. Prevention of microorganism contamination of the instant compositions can be accomplished with various antibacterial and antifungal agents, for example, parabens, chlorobutanol, phenol, sorbic acid and the like. It may also be desirable to include isotonic agents, for example, sugars, sodium chloride and 25 the like. Prolonged absorption of injectable pharmaceutical compositions may be affected by the use of agents capable of delaying absorption, for example, aluminum monostearate and gelatin. For parenteral administration, solutions in sesame or peanut oil, aqueous propylene glycol, or in sterile aqueous solutions may be employed. 30 Such aqueous solutions should be suitably buffered if necessary and the liquid diluent first rendered isotonic with sufficient saline or glucose. These WO 2008/139293 PCT/IB2008/001125 -44- aqueous solutions are especially suitable for intravenous, intramuscular, subcutaneous and intraperitoneal administration. In this connection, the sterile aqueous media employed are all readily available by standard techniques known to those skilled in the art. 5 For intranasal administration or administration by inhalation, the compounds of Formula (I) are conveniently delivered in the form of a solution or suspension from a pump spray container that is squeezed or pumped by the patient or as an aerosol spray presentation from a pressurized container or a nebulizer, with the use of a suitable propellant, e.g., 10 dichlorodifluoromethane, trichlorofluoromethane, dichlorotetrafluoroethane, carbon dioxide or other suitable gas. In the case of a pressurized aerosol, the dosage unit may be determined by providing a valve to deliver a metered amount. The pressurized container or nebulizer may contain a solution or suspension of a compound of this invention. Capsules and cartridges (made, 15 for example, from gelatin) for use in an inhaler or insufflator may be formulated containing a powder mix of a compound or compounds of the invention and a suitable powder base such as lactose or starch. Pharmaceutical compositions of the present invention may also be configured for treatments in veterinary use, where a compound of the present 20 invention, or a veterinarily acceptable salt thereof, or veterinarily acceptable solvate or pro-drug thereof, is administered as a suitably acceptable formulation in accordance with normal veterinary practice and the veterinary practitioner will determine the dosing regimen and route of administration which will be most appropriate for a particular animal. 25 In general, the compounds of Formula (I), and pharmaceutically acceptable salts thereof, may be prepared according to the exemplary routes disclosed in the Schemes and Examples below, as well as by other conventional preparative procedures known, or apparent in light of the instant disclosure, to one of ordinary skill in the art. These processes form further 30 aspects of the invention. WO 2008/139293 PCT/IB2008/001125 -45- Some of the starting compounds for the reactions described in the Schemes and Examples are prepared as illustrated herein. All other starting compounds may be obtained from general commercial sources, such as Sigma-Aldrich Corporation, St. Louis, MO. 5 Unless indicated otherwise, the following experimental abbreviations have the indicated meanings: f.iL - microliter I m - multiplet bd - broad doublet MHz - megahertz bm - broad multiplet Min(s) - minute(s) BOC - f-butoxycarbonyl MeOH - methanol bs - broad singlet Mg - milligram CDCI3 - deuterated chloroform ml - milliliter CD3OD - deuterated methanol mmol - millimoles dd - doublet of doublets MPLC - medium pressure liquid chromatography DMF - dimethylformamide MS - mass spectroscopy DMSO - dimethyl sulfoxide NMR - nuclear magnetic resonance dt - doublet of triplets ppm - parts per million EtOAc - ethyl acetate psi - pounds per square inch EtOH - ethanol s - singlet h (e.g., 1 h, 2h) - hour(s) SPA - scintillation proximity assay H (e.g., 1H, 2H) - hydrogen(s) t - triplet Hz - hertz THF - tetrahydrofuran IPA - isopropyl alcohol Tris -tris(hydroxymethyl)aminomethane J - spin-spin coupling constant LC - liquid chromatography The methods disclosed in the instant Schemes and Examples are intended for purposes of exemplifying the instant invention only and are not to 10 be construed as limitations thereon. WO 2008/139293 PCT/IB2008/001125 -46- Scheme 1 exemplifies multiple ways to form aliphatic hydrazines that can utilized to prepare compounds in this patent. Ketones can be converted to the hydrazide imine and reduced with borane or sodium cyanoborohydride. Other reducing agents can also be utilized. The boc group can then be 5 removed with acid to form the desired hydrazine intermediate. Alternatively aliphatic alcohols can be converted to boc-protected hydrazines by treatment with triphenyl phosphine and di-f-butyl diazacarboxylate. The boc groups can again be removed with acid to liberate the hydrazine. Aromatic hydrazine synthesis is well known in the literature by converting anilines to hydrazines 10 through diazotization chemistry followed by reduction. 15 ((trimethylsilyl)methyl)methanamine which is commercially available by catalysis with acid. This chemistry is exemplified in the experimental section below and also by numerous literature examples such as Hosomi et al., Chem. Lett. 13(7) 1117-1120, 1984. The pyrrolidines have also been synthesized in an enantiomeric pure fashion by either employing chiral 20 auxiliaries on the ester (see Nichols et al., Org. Lett., 8(7), 1495-1498, 2006) or by utilizing a chiral benzyl amine in the cycloaddition chemistry (see Haight et al., Org. Proc. Res. Dev., 8(6), 897-902, 2004). Scheme 1 The pyrrolidine intermediates can be formed by coupling alpha-beta unsaturated esters with N-(methoxymethyl)(phenyl)-N- Scheme 2 WO 2008/139293 PCT/IB2008/001125 -47- C02Me TFA, CH2CI2 N Me3Si r 10 Hydrazines can then be coupled in the presence of a base such as sodium methoxide or triethylamine with 2-(ethoxymethylene)malononitrile or substituted variants to afford the desired amino cyano pyrazoles. The cyano group can be oxidized by a variety of reagent but two conditions have been utilized to prepare compounds for this patent. Concentrated sulfuric acid or hydrogen peroxide with ammonium hydroxide has afforded the amino-amide-pyrazoles. The amino-amide pyrazoles can then be coupled with esters in the presence of a base such as potassium t-butoxide with heated. The solvent of choice for this reaction has been tetrahydrofuran and in some cases dehydrating agents such as molecular sieves can be employed to improve upon the yields of the coupling. Scheme 3 The benzyl group can then be removed via standard hydrogenation conditions to provide the secondary amine that is ready for further functionalization. The amine can be alkylated with alkyl halides in the presence of base or reductive amination chemistry utilizing a variety of hydride reducing agents can provide the desired compounds. Base such as KOtSu in THF 60 °C WO 2008/139293 PCT/IB2008/001125 -48- Scheme 4 Hj, Pd/C or Pd(OH}2 R2CHO, NaBH(OAc)3 or NaCNBH3 R2-X (Br,I,CI, ect.) Base (Cs2C03), DMForTHF H, r-> n HN — A ^2 0 M — N / r2 10 15 EXAMPLES The Examples below are intended to illustrate particular embodiments of the invention and preparations thereto and are not intended to limit the specification, including the claims, in any manner. Unless otherwise noted, all reagents employed were obtained commercially. EXAMPLE 1 (a) 5-amino-1-(2-methoxvphenyl)-1H-pyrazole-4-carbonitrile To a solution of 1-(2-methoxyphenyl)hydrazine hydrogen chloride (3g, 0.017 mol) in ethanol (50 mL) was added 2-(methoxymethylene)malononitrile (1.89 g, 0.9 eq.) and sodium methoxide (1.92g, 2.1 eq). The reaction mixture was heated at reflux for 18h and concentrated. The reaction mixture was partitioned between brine and ethyl acetate. The organic layer was separated, dried with magnesium sulfate, filtered and concentrated. MPLC Biotage chromatography eluting with 20-60% ethyl acetate/hexanes afforded the title compound in 53% yield (1.9g). 400 MHz 1H NMR (CDCI3) 5 7.64 (m, WO 2008/139293 PCT/IB2008/001125 -49- 10 15 1 H), 7.40 (m, 2H), 7.08 (m,2H), 4.51 (bs, 2H), 3.87 (s, 3 H); MS: (M+H m/z = 215.2). (b) 5-amino-1-(2-methoxvphenvl)-1H-p.vrazole-4-carboxamide To a solution of 5-amino-1-(2-methoxyphenyl)-1H-pyrazole-4-carbonitrile (1.53g) in saturated ammonium hydroxide (30 mL) was added 30% hydrogen peroxide solution (6 mL). The reaction stirred for 18h at ambient temperature and was slowly quenched with 60 mL of a saturated sodium sulfate solution. The aqueous layer was extracted with ethyl acetate, dried with magnesium sulfate, filtered and concentrated. MPLC Biotage chromatography eluting with 2-6% methanol/methylene chloride provided the title compound 1,38g (84%). 400 MHz 1H NMR (DMSO) 6 7.79 <s, 1 H), 7.42 (m, 1H), 7.25 (d, J=7.5 Hz, 2H), 7.19 (d, J=8.3 Hz, 2 H), 7.03 (t, J=6.2 Hz, 1H), 5.83 (s,2H), 3.76 (s, 3 H); MS: (M+H m/z = 233.2). (c) (3.4-trans)-methvl 1-benzvl-4-methvlpyrrolidine-3-carboxylate To a solution of (E)-methyl but-2-enoate (1.6 g) was added toluene (30 mL), N-(methoxymethyl) (phenyl)-N-((trimethylsilyl)methyl)methanamine (3.7g) and trifluoroacetic acide (1.5g). The reaction mixture was heated at 50°C for 18h. The reaction mixture was concentrated, quenched with saturated sodium bicarbonate, extracted with methylene chloride, dried with magnesium sulfate, filtered and concentrated. Purification via MPLC chromatography eluting with 20-30% ethyl acetate/hexanes provided the title compound (1.5g). 400 MHz 1H NMR (CDCI3) 5 7.33-7.20 (m, 5H), 4.15-4.08 WO 2008/139293 PCT/IB2008/001125 -50- (m, 1H), 3.66-3.53 (m, 2H), 2.87-2.74 (m, 2H), 2.53-2.44 (m, 2H), 2.23-2.19 (m, 1H), 1.23 (t, J =7.1 Hz, 3H), 1.11 (d, J = 6.6 Hz, 3H). (d) 6-f(3.4-trans)-1-benzyl-4-methylpyrrolidin-3-yn-1-f2-methoxyphenyl)-1.5-dihvdro-4H-pvrazolor3.4-dlpyrimidin-4-one To (3,4-trans)-methyl 1-benzyl-4-methylpyrrolidine-3-carboxylate (241 mg) and 5-amino-1-(2-methoxyphenyl)-1H-pyrazole-4-carboxamide (200mg) was added a solution of potassium t-butoxide in (1M) in THF (4.31 mL, 5 eq.) The reaction mixture was heated at reflux for 16h and poured into saturated 10 sodium bicarbonate. The aqueous layer was extracted with ethyl acetate, dried with magnesium sulfate, filtered and concentrated. MPLC Biotage chromatography eluting with 1-4% methanol/methylene chloride with 0.5% saturated ammonium hydroxide provided 79mg of the title compound. 400 MHz 1H NMR (DMSO) 5 8.16 (d, >7.9 Hz, 1H), 7.92 (s, 1 H), 7.34 (m, 5H), 15 7.09 (m, 1H), 6.92 (m, 2H), 3.89 (s, 3H), 3.84 (m, 1H), 3.71 (m, 1H), 3.37 (t, J=9.1 Hz, 1H), 3.09 (m, 1H), 2.85 (m, 1H), 2.65 (m,1H), 2.47 (m, 1H), 2.03 (m, 2H), 1.19 (d, >7.1 Hz, 3H); MS: (M+H m/z = 416.1). 6-f(3.4-trans)-1-benzvl-4-methvlpyrrolidin-3-vl1-1-cyclopentvl-1.5-dihvdro-4H-20 pvrazolor3.4-dlpyrimidin-4-one Following the procedure for the preparation of 6-[(3,4-trans)-1-benzyl-4-methylpyrrolidin-3-yl]-1 -(2-methoxyphenyl )-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one but substituting 5-amino-1 -cyclopentyl-1 H-pyrazole-4- 5 EXAMPLE 2 WO 2008/139293 PCT/IB2008/001125 -51- carboxamide provided the title compound. 400 MHz 1H NMR (CD3OD) S 8.00 (s, 1H), 7.38-7.23 (m, 5H), 5.14-5.10 (m, 1 H), 3.80-3.57 (m, 2H), 3.34 (t, J = 8.3 Hz, 1H), 2.97 (d, J = 9.9 Hz, 1H), 2.80-2.78 (m, 1H), 2.53-2.49 (m, 1H), 2.41-2.38 (m, 1H), 2.10-1.89 (m, 7H), 1.70-1.66 (m, 2H), 1.17 (d, J = 6.6 Hz, 3H). MS: (M+H m/z = 378.1). EXAMPLE 3 6-ff3S.4S)1-benzvl-4-methvlDvrrolidin-3-vn-1-cvclopentvl-1.5-dih.vdro-4H-pvrazolo[3.4-dlpyrimidin-4-one 10 The racemate was separated on Chiralcel OD chiral HPLC column, Mobile Phase 90/10 Heptane/EtOH, TR = 6.807, to provide the enantiomer. 400 MHz 1H NMR (CDCI3) 5 8.00 (s, 1H), 7.38-7.22 (m, 5H), 5.27-5.10 (m, 1H), 3.78 (d, J= 12.5 Hz, 1H), 3.6 (d, J= 12.5 Hz, 1H), 3.34 (t, J= 8.3 Hz, 15 1H), 2.97 (d, J = 9.9 Hz, 1H), 2.80-2.78 (m, 1H), 2.52-2.48 (m, 1H), 2.41-2.38 (m, 1H), 2.10-1.89 (m, 7H), 1.70-1.66 (m, 2H), 1.18(d, J= 6.6 Hz, 3H). MS: (M+H m/z = 378.1). Chiralcel OD, Mobile Phase 90/10 Heptane/IPA, TR = 9.433. EXAMPLE 4 20 (a) 1-cvclopentvl-6-f(3,4-trans)-4-methvlpvrrolidin-3-vl1-1H-p.vrazolof3.4-dlpvrimidin-4(5H)-one A solution of 6-[(3,4-trans)-1-benzyl-4-methylpyrrolidin-3-yl]-1-cyclopentyl-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one (970mg) in ethanol 25 25 mL was added to a Parr bottle. Acetic acid (2.5 mL) and Pd(OH)2 (500 WO 2008/139293 PCT/IB2008/001125 -52- mg) was added. The reaction mixture was placed on a hydrogenator under 40 PSI for 16h. The reaction mixture was filtered through Celite and concentrated. The reaction mixture was partitioned between saturated bicarbonate solution and methylene chloride. The layers were separated and 5 the aqueous layer was extracted 6x with methylene chloride. The organic layer was dried with magnesium sulfate, filtered and concentrated to provide 429 mg of the title compound. 400 MHz 1H NMR (CD3OD) 5 9.25 (brs, 1H), 8.02 (s, 1H), 5.20-5.17 (m, 1H), 3.91 (m, 1H), 3.77-3.68 (m, 2H), 3.46-3.44 (m, 1H), 3.10 (m, 1H), 2.89 (m,1H), 2.13-1.87 (m, 6H), 1.74-1.65 (m, 2H), 10 1.20 (d, J= 6.2 Hz, 3H). MS: (M+H m/z = 288.1). (b) 1-cvclopentvl-6-((,3.4-trans)-4-methvl-1-r4-(trifluoromethvl)ovrimidin-2-vHDvrrolidin-3-vlH,5-dihydro-4H-pvrazDlof3.4-d1pvrimidin-4-one 15 pyrazolo[3,4-d]pyrimidin-4(5H)-one (40mg) in dimethylformamide (1ml) was added cesium carbonate (2eq.) and 2-chloro-4-(trifluoromethyl)pyrimidine (1.2 eq.) and the reaction mixture was heated at 60 °C for 90 min. The reaction mixture was poured into saturated sodium bicarbonate, extracted with methylene chloride, dried with magnesium sulfate, filtered and concentrated. 20 Purification via MPLC Biotage chromatography eluting with 20-60% ethyl acetate/hexanes provided 40 mg cf the title compound. 400 MHz 1H NMR (CDCI3) 5 8.51 (d, J = 5.0 Hz, 1H), 8.02 (m, 1H), 6.79 (d, J = 4.6 Hz, 1H), 5.16-5.08 (m, 1H), 4.243.97 (m, 3H), 3.37-3.32 (m, 1H), 3.20-3.14 (m, 1H), 2.09-2.05 (m, 3H), 1.96-1.90 (m, 1H), 1.73-1.56 (m, 5H), 1.24 (d, J = 6.6 Hz, 25 3H). MS: (M+H m/z = 434.1). To a solution of 1-cyclopentyl-6-[(3,4-trans)-4-methylpyrrolidin-3-yl]-1H- WO 2008/139293 PCT/IB2008/001125 -53- EXAMPLE 5 1-cyclopentvl-6-f(3.4-trans)-4-methyl-1-pyrimidin-2-vlpyrrolidin-3-yl1-1,5-dihvdro-4H-pyrazolof3,4-dlpyrimidin-4-one [Rac) 5 Following the procedure for the preparation of 1-cyclopentyl-6-{(3,4- trans)-4-methyl-1-[4-(trifluoromethyl)pyrimidin-2-yl]pyrrolidin-3-yl}-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one but substituting 2-chloropyrimidine provided the title compound. 400 MHz 1H NMR (CD3OD) 5 8.29 (d, J = 5.0 Hz, 2H), 7.96 (s,1H), 6.60 (t, J = 5.0 Hz, 1H), 5.08-5.04 (m, 1H), 4.06-3.87 (m, 10 3H), 3.23-3.18 (m, 1H), 3.12 (q, J = 7.9 Hz, 1H), 2.80-2.76 (m, 1H), 2.05-1.98 (m,4H), 1.90-1.82 (m, 2H), 1.66-1.61 (m, 2H), 1.17 (d, J = 7.1 Hz, 3H). MS: (M+H m/z = 366.1). 6-[(3.4-trans)-1 -benzovl-4-methvlpyrrolidin-3-yl1-1 -cyclopentyl-1,5-dihvdro-4H-15 pyrazolof3.4-dlpvrimidin-4-one To a solution of 1-cyclopentyl-6-[(3,4-trans)-4-methylpyrrolidin-3-yl]-1H-25 pyrazolo[3,4-d]pyrimidin-4(5H)-one (40 mg) in methylene chloride (1 ml) was added triethyl amine (2.5 eq.) and benzoyl chloride (1.2 eq.) and the reaction mixture was stirred at ambient temperature for 1h. The reaction mixture was poured into saturated sodium bicarbonate, extracted with methylene chloride, EXAMPLE 6 n WO 2008/139293 PCT/IB2008/001125 -54- dried with magnesium sulfate, filtered and concentrated. Biotage MPLC chromatography eluting with 2-4% methanol/methylene chloride provided the title compound (27mg). 400 MHz 1H NMR (CDCI3) 5 8.06-8.03 (m, 1H), 7.57-7.50 (m, 2H), 7.43-7.37 (m, 3H), 5.20-5.13 (m, 1H), 4.16^.03 (m, 1H), 3.92 5 (d, J = 8.3 Hz, 1H), 3.81-3.76 (m, 1H), 3.47-3.29 (m, 1H), 3.17-3.03 (m, 1H), 2.84-2.67 (m, 1H), 2.11-1.80 (m, 3H), 1.79-1.72 (m, 1H), 1.56-1.30 (m, 4H), 1.21-1.11 (m, 3H). MS: (M+H m/z = 392.1). 1-cvclopentvl-6-ff3.4-trans)-4-methvl-1-(pyridin-3-vlmethvl)pyrrolidin-3-vn-1.5-10 dihvdro-4H-Pvrazolof3.4-dlPvrimidin-4-one To a solution of 1-cyclopentyl-6-[(3,4-trans)-4-methylpyrrolidin-3-yl]-1H- pyrazolo[3,4-d]pyrimidin-4(5H)-one (40 mg) in 1,2-dichloroethane (2 mL) was 20 added acetic acid (2 eq.), nicotinaldehyde (1.5 eq.) and sodium triacetoxy borohydride (58 mg). The reaction mixture was heated at 40 °C for 4h, poured into saturated sodium bicarbonate, extracted with methylene chloride, dried with magnesium sulfate, filtered and concentrated. Purification via Biotage MPLC chromatography eluting with 1-4% methanol/methylene 25 chloride/0.5% ammonium hydroxide provided the title compound (47 mg). 400 MHz 1H NMR (CDCI3) 5 8.55 (m, 2H), 2.02 (s, 1H), 7.84 (m,1H), 7.36 (m, 1H), 5.16-5.09 (m, 1H), 3.82-3.60 (m, 2H), 3.36 (m,1H), 3.05-2.38 (m, 4H), 2.13-1.89 (m, 7H), 1.73-1.68 (m, 2H), 1.21 (m, J =7.1 Hz, 3H). MS: (M+H m/z = 379.1). 30 EXAMPLE 8 1-cvclopentvl-6-(f3,4-trans)-4-methvl-1-f3-ftrifluoromethvl)benzvl]pyrrolidin-3- vll-1.5-dihydro-4H-pyrazolof3.4-dlpyrimidin-4-one EXAMPLE 7 WO 2008/139293 PCT/IB2008/001125 -55- Following the procedure for the preparation of 1-cyclopentyl-6-{(3,4- trans)-4-methyl-1-(pyridin-3-ylmethyl)pyrrolidin-3-yl]-1,5-dihydro-4H- pyrazolo[3,4-d]pyrimidin-4-one but substituting 3- 5 (trifiuoromethyl)benzaldehyde provided the title compound. 400 MHz 1H NMR (CDCI3) 5 8.02 (s, 1H), 7.66 (s,1H), 7.53-7.50 (m, 3H), 5.16-5.09 (m, 1H), 3.80 (m, 1H), 3.69-3.66 (m, 1H), 3.35 (m, 1H), 2.99<m,1H), 2.83 (m,1H), 2.42 (m, 1H), 2.12-1.93 (m, 7H), 1.74-1.68 (m, 2H), 1.56 (m, 1H), 1.21 (d, J = 6.6 Hz, 3H). MS: (M+H m/z = 446.0). 10 EXAMPLE 9 1-cvclopentvl-6-IY3.4-trans)-4-methvl-1-(auinolin-2-vlmethvl)pvrrolidin-3-vll- 1.5-dihvdro-4H-pvrazolor3.4-d1pyrimidin-4-one fRac) 15 trans)-4-methyl-1-(pyridin-3-ylmethyl)pyrrolidin-3-yl]-1,5-dihydro-4H- pyrazolo[3,4-d]pyrimidin-4-one but substituting quinoline-2-carbaldehyde provided the title compound. 400 MHz 1H NMR (CDCI3) 5 8.22-8.15 (m, 2H), 8.07 (s, 1H), 7.80 (d, J = 7.9 Hz, 1H), 7.71 (t, J = 8.3 Hz, 1H), 7.69-7.50 (m, 2H), 5.18-5.11 (m, 1H), 4.25 (m, 1H), 3.91 (m, 1H), 3.71 (m, 1H), 3.49 (m, 20 1H), 3.17 (m, 1H), 2.87 (m, 1H), 2.73-2.45 (m, 2H), 2.13-1.94 (m, 6H), 1.75-1.68 (m, 2H), 1.23 (d, J = 7.1Hz, 3H). MS: (M+H m/z = 429.1). 1-cvclopentvl-6-f('3,4-trans)-4-methvl-1-(quinolin-4-vlmethvl)pvrrolidin-3-vl1-1.5-dihvdro-4H-pvrazolor3.4-dlpyrimidin-4-one Following the procedure for the preparation of 1-cyclopentyl-6-[(3,4- EXAMPLE 10 WO 2008/139293 PCT/IB2008/001125 -56- Following the procedure for the preparation of 1-cyclopentyl-6-[(3,4- trans)-4-methyl-1-(pyridin-3-ylmethyl)pyrrolidin-3-yl]-1,5-dihydro-4H- pyrazolo[3,4-d]pyrimidin-4-one but substituting quinoline-4-carbaldehyde 5 provided the title compound. 400 MHz 1H NMR (CDCI3) 5 8.92-8.89 (m, 1H), 8.27-8.25 (m, 1H), 8.18-8.16 (m, 1H), 8.00 (s,1H), 7.80-7.74 (m, 2H), 7.63- 7.50 (m,1H), 5.14-5.07 (m, 1H), 4.18 (m, 2H), 3.37 (m, 1H), 3.10-2.30 (m, 5H), 2.15-1.93 (m, 6H), 1.74-1.64 (m, 2H), 1.21 (d, J = 6.6 Hz, 3H). MS: (M+H m/z = 429.1). 10 EXAMPLE 11 1-cvclopentvl-6-[(3.4-trans)-4-methvl-1-(f6-(trifluoromethvl)pyridin-3- vl]methvl>pvrrolidin-3-vn-1.5-dihvdro-4H-pvrazolof3,4-dlpyrimidin-4-one 15 trans)-4-methyl-1 -(pyridin-3-ylmethyl)pyrrolidin-3-yl]-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one but substituting 6- (trifiuoromethyl)nicotinaldehyde provided the title compound. 400 MHz 1H NMR (CDCI3) 5 8.63 (m, 1H), 8.01 (s, 1H), 7.7 (d, J= 7.80 Hz, 1H), 5.12 (m, 1H), 4.82 (m, 1H), 3.79 (q, J=13.2, 16.2, Hz, 2H), 3.32 (t, J=8.5 Hz, 1H), 3.02 20 (m, 1H), 2.86 (m, 1H), 2.63 (m, 1H), 2.45 (m, 1H), 2.12-2.88 (m, 6H), ), 1.74-1.64 (m, 3H), 1.32 (d, J= 7.05 Hz, 3H). MS: (M+H m/z = 447.0). Following the procedure for the preparation of 1-cyclopentyl-6-[(3,4- WO 2008/139293 PCT/IB2008/001125 -57- EXAMPLE 12 1-cvclopentvl-6-ff3.4-trans)-4-methvl-1-(Quinoxalin-2-vlmethyl)pvrrolidin-3-vn-1.5-dihydro-4H-pyrazolor3,4-dlpyrimidin-4-one 5 Following the procedure for the preparation of 1-cyclopentyl-6-[(3,4- trans)-4-methyl-1-(pyridin-3-ylmethyl)pyrrolidin-3-yl]-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one but substituting quinoxaline-2-carbaldehyde provided the title compound. 400 MHz 1H NMR (CDCI3) 5 8.86 (s, 1H), 8.31-8.29 (m, 1H), 8.05 - 8.08 (m, 2H), 7.79-7.70 (m, 2H), 5.16-5.12 (m, 1H), 4.32-10 4.28 (m, 1H), 3.94-3.98 (m, 1H), 3.46 (t, J = 8.3 Hz, 1H), 3.26 (d, J = 9.5 Hz, 1H), 2.90-2.88 (m, 1H), 2.64-2.60 (m, 1H), 2.50-2.47 (m, 1H), 2.18 (t, J = 8.3 Hz, 1H), 2.11-1.91 (m, 6H), 1.71-1.66 (m, 2H), 1.23 (d, J= 7.05 Hz, 3H). MS: (M+H m/z = 430.1). EXAMPLE 13 15 1-cvclopentvl-6-f(3.4-trans)-4-methvl-1-(auinoxalin-6-vlmethvl)pvrrolidin-3-vl1-1.5-dihvdro-4H-pvrazolor3.4-dlpyrimidin-4-one Following the procedure for the preparation of 1-cyclopentyl-6-[(3,4-trans)-4-methyl-1-(pyridin-3-ylmethyl)pyrrolidin-3-yl]-1,5-dihydro-4H-20 pyrazolo[3,4-d]pyrimidin-4-one but substituting quinoxaline-6-carbaldehyde provided the title compound. 400 MHz 1H NMR (CDCI3) 5 8.82 (s, 2H), 8.14 (d, J = 8.3 Hz, 1H), 8.07-7.93 (m, 3H), 5.15-5.08 (m, 1H), 4.00-3.87 (m, 2H), 3.37 (t, J= 8.7 Hz, 1H), 3.04 (d, J = 9.5 Hz, 1H), 2.85-2.84 (m, 1H), 2.66- WO 2008/139293 PCT/IB2008/001125 -58- 2.62 (m, 1H), 2.46-2.42 (m, 1H), 2.11-1.92 (m, 7H), 1.71-1.63 (m, 2H), 1.20 (d, J= 7.05 Hz, 3H). MS: (M+H m/z = 430.1). EXAMPLE 14 1-cvcloDentvl-6-ff3.4-trans)-4-methvl-1-(pvrimidin-5-vlmethvl)pvrrolidin-3-vll-5 1.5-dihvdro-4H-pvrazolor3.4-dlpyrimidin-4-one Following the procedure for the preparation of 1-cyclopentyl-6-[(3,4-trans)-4-methyl-1-(pyridin-3-ylmethyl)pyrrolidin-3-yl]-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one but substituting pyrimidine-5-carbaldehyde 10 provided the title compound. 400 MHz ^ NMR, (CDCI3) 5 9.16 (s, 1H), 8.76 (s, 2H), 8.02 (s,1H), 5.17-5.10 (m, 1H), 3.78-3.69 (m, 2H), 3.27 (t, J = 8.7 Hz, 1H), 3.07 (d, J = 9.5 Hz, 1H), 2.89 (m,1H), 2.69 (m, 1H), 2.48-2.46 (m, 1H), 2.12-1.89 (m, 6H), 1.74-1.63 (m, 3H), 1.26-1.19 (m, 3H). MS: (M+H m/z = 380.1). 15 EXAMPLE 15 1-cvclopentvl-6-[(3,4-trans)-1,4-dimethvlpyrrolidin-3-vn-1,5-dihvdro-4H- pvrazolor3.4-dlpyrimidin-4-one @H) Following the procedure for the preparation of 1-cyclopentyl-6-[(3,4- 20 trans)-4-methyl-1-(pyridin-3-ylmethyl)pyrrolidin-3-yl]-1,5-dihydro-4H- pyrazolo[3,4-d]pyrimidin-4-one but substituting formaldehyde provided the title compound. 400 MHz 1H NMR, (CDCI3) 5 8.04 (s, 1H), 5.18-5.11 (m, 1H), 3.39 (m,1H), 3.15 (m,1H), 3.03-2.96 (m, 1H), 2.70 (m,1H), 2.51 (m, 4H), WO 2008/139293 PCT/IB2008/001125 59- 2.15-1.90 (m, 7H), 1.75-1.66 (m, 2H), 1.20 (d, J = 6.6 Hz, 3H). MS: (M+H m/z = 302.2). EXAMPLE 16 1-cvclopentvl-6-f(3,4-trans)~4-methvl-1-(2.2.2-trifluoroethvl)pvrrolidin-3-vn-1.5-5 dihvdro-4H-pvrazolor3.4-dlpvrimidin-4-one [ Rac] To a solution of 1-cyclopentyl-6-[(3,4-trans)-4-methylpyrrolidin-3-yl]-1H- pyrazolo[3,4-d]pyrimidin-4(5H)-one (40 mg) in dimethyl formamide (1.5mL) was added sodium carbonate (30 mg) and 1,1,1 -trifluoro-2-iodoethane (1.1 10 eq.). The reaction mixture was heated at 40 °C for 3 days. An additional 3 eq. of 1,1,1 -trifluoro-2-iodoethane along with cesium carbonate (2eq.) and the reaction mixture was heated at 60 °C for 3 days. The reaction mixture was poured into saturated sodium bicarbonate, extracted with methylene chloride, dried with magnesium sulfate, filtered and concentrated. Purification via 15 MPLC Biotage eluting with 0.5-2% methanol/methylene chloride/0.5% ammonium hydroxide provided the title compound (9mg). 400 MHz 1H NMR, (CDCIj) 5 8.03 (s, 1H), 5.16-5.10 (m, 1H), 3.50-3.48 (m, 1 H), 3.30-3.25 (m, 2H), 2.52 (m,1H), 2.25 (m,1H), 2.13-1.94 (m, 5H), 1.72-1.57 (m, 6H), 1.22 (d, J -7 A Hz, 3H). MS: (M+H m/z = 370.1). 20 EXAMPLE 17 1-cvclopentvl-6-(f3.4-trans)-4-methvl-1-[(2-methvlpvridin-3- vnmethvl]pvrrolidin-3-vl>-1.5-dihvdro-4H-pvrazolof3.4-d]pyrimidin-4-one WO 2008/139293 PCT/IB2008/001125 -60- Following the procedure for the preparation of 1-cyclopentyl-6-[(3,4-trans)-4-methyl-1-(pyridin-3-ylmethyl)pyrrolidin-3-yl]-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one but substituting 2-methylnicotinaldehyde provided the title compound. 400 MHz 1H NMR (CDCI3) 6 8.40-8.39 (m, 1H), 5 7.99 (s, 1H), 7.62-7.60 (m, 1H), 7.12-7.09 (m, 1H), 5.15-5.08 (m, 1H), 3.73-3.65 (m, 2H), 3.30 (t, J = 8.7 Hz, 1H), 3.04 (d, J = 9.95 Hz, 1 H), 2.86-2.84 (m, 1H), 2.66-2.62 (m, 4H), 2.46-2.39 (m, 1H), 2.11-1.89 (m, 7H), 1.71-1.64 (m, 2H), 1.19 (d, J= 7.05 Hz, 3H). MS: (M+H m/z = 393.2). 10 1-cvclopentvl-6-[f3.4-trans)-4-methvl-1-(Quinolin-8-vlmethvl)pvrrolidin-3-vl1-1.5-dihvdro-4H-pvrazolor3.4-dlpyrimidin-4-one Following the procedure for the preparation of 1-cyclopentyl-6-[(3,4-trans)-4-methyl-1-(pyridin-3-ylmethyl)pyrrolidin-3-yl]-1,5-dihydro-4H-15 pyrazolo[3,4-d]pyrimidin-4-one but substituting quinoline-5-carbaldehyde provided the title compound. 400 MHz 1H NMR (CDCI3) 5 9.10-9.09 (m, 1H), 8.13 (dd, J = 8.3, 1.6 Hz, 1H), 7.98 (s, 1H), 7.76-7.74 (d, J= 7.88 Hz, 2H), 7.50 (t, J= 7.88 Hz, 1H), 7.45-7.42 (m, 1H), 5.14-5.07 (m, 1H), 4.52 (d, J= 12.40 Hz, 1H), 4.21 (d, J= 12.40 Hz, 1 H), 3.34 <t, J = 8.3 Hz, 1H), 3.1 (d, J = 20 9.95 Hz, 1H), 2.8-2.79 (m, 1H), 2.71-2.67 (m, 1H), 2.37-2.31 (m, 1H), 2.10-1.88 (m, 7H), 1.72-1.62 (m, 2H), 1.17 (d, J= 6.64 Hz, 3H). MS: (M+H m/z = 429.2). 1-cvclopentvl-6-r(3.4-trans)-4-methvl-1-(Quinolin-3-vlmethvl)pyrrolidin-3-vl1- EXAMPLE 18 [Rac] EXAMPLE 19 25 1.5-dihvdro-4H-pyrazolor3.4-dlPvrimidin-4-one WO 2008/139293 PCT/IB2008/001125 -61- [RacJ trans)-4-methyl-1-(pyridin-3-ylmethyl)pyrrplidin-3-yl]-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one but substituting quinoline-3-carbaldehyde 5 provided the title compound. 400 MHz 1H NMR (CDCI3) 5 8.87 (s, 1H), 8.29 (s, 1H), 8.07 (d, J= 7.8 Hz, 1H), 8.02 (s,1H), 7.89 (d, J= 7.9 Hz, 1H), 7.70-7.66 (m, 1H), 7.54 (t, J= 7.5 Hz, 1H), 5.16-5.08 (m, 1H), 4.02-3.98 (m, 1H), 3.86-3.83 (m, 1H), 3.47 (s, 1H), 3.40 (t, J= 8.3 Hz, 1H), 3.07 (d, J = 8.5 Hz, 1H), 2.85 (m, 1H), 2.65-2.61 (m, 1H), 2.47-2.46 (m, 1H), 2.15-1.87 (m, 6H), 10 1.73-1.63 (m, 2H), 1.20 (d, J= 7.1 Hz, 3H). MS: (M*H m/z = 429.2). EXAMPLE 20 1-cvclopentvl-6-(f3.4-transM-methvl-1-[(6-methvlpyridin-3-vl)methvnpvrrolidin-3-vl)-1.5-dihvdro-4H-pvrazolof3.4-dlpyrimidin-4-one [Rac] 15 Following the procedure for the preparation of 1-cyclopentyl-6-[(3,4- trans)-4-methyl-1 -(pyridin-3-y Imethyl )pyrrol idin-3-yl]-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one but substituting 6-methylnicotinaldehyde provided the title compound. 400 MHz 1H NMR (CDCI3) 5 8.37 (d, J= 1.2 Hz, 1H), 8.00 (s, 1H), 7.73 (dd, J= 7.9, 2.1 Hz, 1H), 7.18 (d, J= 8.3 Hz, 1H), 20 5.16-5.08 (m, 1H), 3.76-3.72 (m, 1H), 3.61-3.57 (m, 1H), 3.32 (t, J= 8.71 Hz, 1H), 2.98 (d, J = 9.9 Hz, 1H), 2.83-2.81 (m, 1H), 2.57-2.51 (m, 4H), 2.42-2.38 (m, 1H), 2.11-1.89 (m, 7H), 1.72-1.64 (m, 2H), 1.19 (d, J= 7.1 Hz, 3H). MS: (M+H m/z = 393.2). WO 2008/139293 PCT/IB2008/001125 -62- EXAMPLE 21 (a) f3.4-trans)-methyl 1-benzvl-4-isopropvlpvrrolidine-3-carboxv]ate Following the procedure for the preparation of (3,4-trans)-methyl 1- 5 benzyl-4-methyl pyrrolidine-3-carboxylate but substituting (E)-methyl 4-methylpent-2-enoate provided the title compound. 400 MHz 1H NMR (CDCI3) 5 7.31-7.20 (m, 5H), 3.66 (s, 3H), 3.62-3.49 (m, 2H), 2.79-2.69 (m, 3H), 2.31-2.27 (m, 2H), 1.61-1.56 (m, 1H), 1.27-4.25 (m, 1H), 0.86 (t, J =2.9 Hz, 6H). MS: (M+H m/z = 262.2). 10 (b) 6-r(3.4-trans)-1-benzvl-4-isopropvlpyrrolidin-3-vl1-1-cvclopentvl-1.5- dihvdro-4H-pvrazolof3.4-dlpyrimidin-4-one Following the procedure for the preparation of 6-[(3,4-trans)-1-benzyl-4-methylpyrrolidin-3-yl]-1-(2-methoxyphenyl)-1,5-dihydro-4H-pyrazolo[3,4-15 d]pyrimidin-4-one but substituting 5-amino-1-cyclopentyl-1H-pyrazole-4-carboxamide and (3,4-trans)-methyl 1-benzyl-4-isopropylpyrrolidine-3-carboxylate provided the title compound. 400 MHz 1H NMR (CDCI3) 5 8.00 (s, 1H), 7.38-7.32 (m, 4H), 7.27-7.24 (m, 1H), 5.13-5.05 (m, 1H), 3.78 (d, J= 12.5 Hz, 1H), 3.6 (d, J= 12.5 Hz, 1H), 3.27-3.21 (m, 1H), 2.98-2.96 (m, 20 2H), 2.37 (m, 1H), 2.10-1.88 (m, 7H), 1,69-1.60 (m, 3H), 0.98 (d, J= 6.6 Hz, 3H), 0.86 (d, J= 6.6 Hz, 3H). MS: (M+H m/z = 406.1). [Rac] EXAMPLE 22 (a) 5-amino-1-cvclopentvl-3-methvl-1H-pyrazole-4-carbonitrile WO 2008/139293 PCT/IB2008/001125 -63- 6 Following the procedure for the preparation of 5-amino-1-(2-methoxyphenyl)-1H-pyrazole-4-carbonitrile but substituting 1- 5 cyclopentylhydrazine and 2-(1-methoxyethylidene)malononitrile provided the title compound. 1H NMR (300 MHz, CDCI3): 5 4.24 (m, 3H), 2.24 (s, 3H), 2.01 (m, 4H), 1.90 (m, 2H), 1.67 (m, 2H). (b) 5-amino-1 -cvclopentvl-3-methvl-l H-pyrazole-4-carboxamide methoxyphenyl)-1H-pyrazoie-4-carboxamide but substituting 5-amino-1-cyclopentyl-3-methyl-1 H-pyrazole-4-carbonitrile provided the title compound. 1H NMR (300 MHz, CDCI3): 5 5.39 (br, 2H), 5.32 (br, 2H), 4.26 (m, 1H), 2.38 (s, 3H), 2.04 (m, 4H), 1.9 (m, 2H), 1.66 (m, 2H). 15 (c) 6-r(3,4-trans)-1-benzvl-4-methylpyrrolidin-3-vn-1-cyclopentyl-3-methvl-1.5-dihvdro-4H-pvrazolor3.4-dlpyrimidin-4-one Following the procedure for the preparation of 6-[(3,4-trans)-1-benzyl-4-methylpyrrolidin-3-yl]-1-(2-methoxyphenyl)-1,5-dihydro-4H-pyrazolo[3,4-20 d]pyrimidin-4-one but substituting 5-amino-1-cyclopentyl-3-methyl-1 H- 10 Following the procedure for the preparation of 5-amino-1-(2- .Rac: WO 2008/139293 PCT/IB2008/001125 -64- pyrazole-4-carboxamide provided the title compound. 400 MHz 1H NMR (CDCIs) 6 7.45-7.20 (m, 5H), 5.13-4.96 (m, 1H), 3.85-3.61 (m, 2H), 3.45-3.30 (m, 1H), 3.08-2.98 (m, 1H), 2.82-2.74 (m, 1H), 2.60-2.45 (m, 4H), 2.45-2.30 (m, 1H), 2.18-1.80 (m, 6H), 1.79-1.50 (m, 3H), 1.2-1.1 (m, 3H). 5 MS: (M+H m/z = 392.5). EXAMPLE 23 (a) 1-cvcloDentvl-6-f(3S.4S)-4-methvlpyrrolidin-3-vl1-1H-pvrazolor3.4- dlpvrimidin-4(5H)-one 10 A solution of 6-[(3S,4S)1-benzyl-4-methylpyrrolidin-3-yl]-1-cyclopentyl- 1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one (9.25g) in ethanol 100 mL was added to a Parr bottle. 2 mL of concentrated HCI followed by 3g of palladium hydroxide was added. The reaction mixture was placed on a hydrogenator under 45 psi of H2 gas for 4h. The reaction mixture was filtered through Celite 15 and concentrated to provide the title compound as an HCI salt. 400 MHz 1H NMR (CDCI3) 5 8.00 (s, 1H), 5.14-5.10 (m, 1H), 4.89-3.84 (m, 1H), 3.72-3.67 (m,1H), 3.38-3.31 (m, 1H), 3.03-2.98 (m, 1H), 2.85-2.81 (m, 1H), 2.08-1.85 (m,7H), 1.69-1.61 (m, 2H), 1.19-1.10 (m, 3H). MS: (M'H m/z = 288.2). (b) 1-cvclopentyl-6-[(3S.4S)-4-methvl-1-(auinoxalin-6-vlmethvl)pyrrolidin-3-yl1-20 1.5-dihvdro-4H-pyrazolo[3,4-dlpyrimidin-4-one Following the procedure for the preparation of 1-cyclopentyl-6-[(3,4-trans)-4-methyl-1-(pyridin-3-ylmethyl)pyrrolidin-3-yl]-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one but substituting 1-cyclopentyl-6-[(3S,4S)-4- WO 2008/139293 PCT/IB2008/001125 -65- methylpyrrolidin-3-yl]-1 H-pyrazolo[3,4-d]pyrimidin-4(5H)-one and quinoxaline-6-carbaldehyde provided the title compound. 400 MHz 1H NMR (CDCI3) 5 8.82 (s, 2H), 8.14 (d, J= 8.3 Hz, 1H), 8.07-7.93 (m, 3H), 5.15-5.08 <m, 1H), 4.00-3.87 (m, 2H), 3.37 (t, J = 8.7 Hz, 1H), 3.04 (d, J = 9.5 Hz, 1H), 2.85-5 2.84 (m, 1H), 2.66-2.62 (m, 1H), 2.46-2.42 (m, 1H), 2.11-1.92 (m, 7H), 1.71-1.63 (m, 2H), 1.20 (d, J= 7.05 Hz, 3H). MS: (M+H m/z = 430.1). 1-cvclopentvl-6-f(3.4-trans)-4-methvl-1-(2-phenvlethvl)pyrrolidin-3-vll-1.5-dihvdro-4H-pyrazolor3.4-dlpyrimidin-4-one Following the procedure for the preparation of 1-cyclopentyl-6-[(3,4-trans)-4-methyl-1-(pyridin-3-ylmethyl)pyrrolidin-3-yl]-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one but substituting 2-phenylacetaldehyde provided the title compound. 400 MHz 1H NMR (CDCI3) 5 8.03 (s, 1H), 7.32-15 7.19 (m, 5H), 5.18-5.11 (m, 1H), 3.44 (t, J= 8.7 Hz, 1H), 3.14 (d, J = 9.9 Hz, 1H), 2.90-2.78 (m, 5H), 2.56-2.52 (m, 1H), 2.40-2.39 (m, 1H), 2.14-1.89 (m, 7H), 1.73-1.67 (m, 2H), 1.19 (d, J= 7.05 Hz, 3H). MS: (M+H m/z = 392.1). 1-cvclopentvl-6-((3.4-trans)-1-r(6-methoxvpvridin-3-vl)methvll-4-20 methvlPvrrolidin-3-vl>-1.5-dihvdro-4H-pvrazolof3.4-dlpvnmidin-4-one EXAMPLE 24 10 EXAMPLE 25 WO 2008/139293 PCT/IB2008/001125 -66- Following the procedure for the preparation of 1-cyclopentyl-6-[(3,4- trans)-4-methyl-1-(pyridin-3-ylmethyl)pyrrolidin-3-yl]-1,5-dihydro-4H- pyrazolo[3,4-d]pyrimidin-4-one but substituting 6-methoxynicotinaldehyde provided the title compound. 400 MHz 1H NMR (CDCI3) 5 8.01 (dd, J = 31.5, 5 2.5 Hz, 1H), 8.00 (s, 1H), 7.69 (dd, J = 8.3, 2.5 Hz, 1H), 6.76 (d, J = 8.3 Hz, 1H), 5.16-5.09 (m, 1H), 3.90 (s, 3H), 3.68 (d, J = 12.9 Hz, 1H), 3.55 (d, J = 12.9 Hz, 1H), 3.31 (t, J= 8.7 Hz, 1H), 2.98 (d, J = 9.9 Hz, 1H), 2.81-2.79 (m, 1H), 2.54-2.50 (m, 1H), 2.41-2.36 (m, 1 H), 2.12-1.87 (m, 7H), 1.73-1.64 (m, 2H), 1.18 (d, J= 7.05 Hz, 3H). MS: (M+H m/z = 409.1). 10 EXAMPLE 26 1-cvclopentvl-6-f(3.4-trans)-4-methvl-1-(Pvridin-2-vlmethvQpvrrolidin-3-vn-1.5- dihvdro-4H-pvrazolor3.4-dlpyrimidin-4-one [Rac; 0 Following the procedure for the preparation of 1-cyclopentyl-6-[(3,4-15 trans)-4-methyl-1-(pyridin-3-ylmethyl)pyrrolidin-3-yl]-1,5-dihydro-4H- pyrazolo[3,4-d]pyrimidin-4-one but substituting picolinaldehyde provided the title compound. 400 MHz 1H NMR (CDCI3) 5 8.63-8.62 (m, 1H), 8.02 (s, 1H), 7.72-7.67 (m, 1H), 7.41 (d, J = 7.9 Hz, 1H), 7.20-7.17 (m, 1H), 5.18-5.10 (m, 1H), 4.03 (d, J =13.7 Hz, 1H), 3.75 (d, J =13.7 Hz, 1H), 3.41 (t, J= 8.3 Hz, 20 1H), 3.07 (d, J = 9.9 Hz, 1H), 2.83-2.82 (m, 1H), 2.58 (m, 1H), 2.46-2.40 25 1-cvclopentvl-6-((3.4-trans)-4-methvl-1-f(3-methvlpvridin-2-vnmethvllpyrrolidin-3-vlV1.5-dihvdro-4H-pvrazolof3.4-dlpyrimidin-4-one WO 2008/139293 PCT/IB2008/001125 -67- Following the procedure for the preparation of 1-cyclopentyl-6-[(3,4-trans)-4-methyl-1-(pyridin-3-ylmethyl)pyrrolidin-3-yl]-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one but substituting 3-methylpicolinaldehyde 5 provided the title compound. 400 MHz 1H NMR (CDCI3) 5 8.02 (s, 1H), 7.57 (t, J= 7.5, 1H), 7.22 (d, J= 7.8 Hz, 1H), 7.02 (d, J = 7.5 Hz, 1H), 5.17-5.09 (m, 1H), 3.97 (d, J = 13.3 Hz, 1H), 3.68 (d, J = 13.3 Hz, 1H), 3.42 (t, J= 8.3 Hz, 1H), 3.05 (d, J = 9.9 Hz, 1H), 2.82-2.80 (m, 1H), 2.57-2.53 (m, 1H), 5.54 (s, 3H), 2.44-2.38 (m, 1H), 2.12-1.89 (m, 7H), 1.72-1.64 <m, 2H), 1.19 10 (d, J= 7.05 Hz, 3H). MS: (M+H m/z = 393.1). Following the procedure for the preparation of (3,4-trans)-methyl 1- 15 benzyl-4-methylpyrrolidine-3-carboxylate but substituting (E)-methyl pent-2-enoate provided the title compound. 400 MHz 1H NMR (CDCI3) 5 7.33-7.19 (m, 5H), 4.15-4.08 (m, 2H), 3.65-3.52 (m, 2H), 2.82-2.71 (m, 3H), 2.60-2.55 (m, 1H), 2.38-2.24 (m, 2H), 1.59-1.50 (m, 1 H), 1.47-1.38 (m, 1H), 1.23 (t, J = 7.5 Hz, 3H), 0.87 (t, J = 7.1 Hz, 3H). MS: (M+H m/z = 262.2). 20 (b) 6-r(3.4-trans)-1-benzvl-4-ethvlpyrrolidin-3-yn-1-cvclopentyl-1,5-dihvdro-4H-Pvrazolor3.4-dlPvrimidin-4-one EXAMPLE 28 (a) f3.4-trans)-ethvl 1 -benzvl-4-ethvlpyrrolidine-3-carboxvlate WO 2008/139293 PCT/IB2008/001125 -68- ("Rac; Following the procedure for the preparation of 6-[(3,4-trans)-1 -benzyl-4-methylpyrrolidin-3-yl]-1-(2-methoxyphenyl)-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one but substituting 5-amino-1-cyclopentyl-1 H-pyrazole-4-5 carboxamide and (3,4-trans)-methyl 1-benzyl-4-ethylpyrrolidine-3-carboxylate provided the title compound. 400 MHz 1H NMR (CDCI3) 6 8.00 (s, 1H), 7.39-7.24 (m, 5H), 5.13-5.07 (m, 1H), 3.78 (d, J = 13.3 Hz, 1H), 3.58 (d, J = 12.9 Hz, 1H), 3.42 (t, J= 12.9 Hz, 1H), 3.33 (t, J = 8.7 Hz, 1H), 2.98 (d, J = 9.9 Hz, 1H), 2.86 (m, 1H), 2.46-2.42 (m, 1H), 2.19-1.44 (m,11H), 0.92 (t, J= 10 7.05 Hz, 3H). MS: (M+H m/z = 392.1). EXAMPLE 29 (a) f3.4-trans)-methvl-1-benzvl-4-cvclopropvlpvrrolidine-3-carboxvlate Following the procedure for the preparation of (3,4-trans)-methyl 1-15 benzyl-4-methylpyrrolidine-3-carboxylate but substituting (E)-methyl 3- (b) 6-f(3.4-trans)-1-benzvl-4-cvclopro dihvdro-4H-pvrazolof3.4-dlpyrimidin-4-one WO 2008/139293 PCT/IB2008/001125 ' - w w » w W f / £ -69- Following the procedure for the preparation of 6-[(3,4-trans)-1-benzyl-4-methylpyrrolidin-3-yi]-1 -(2-methoxyphenyl)-1,5-dihydro-4H-pyrazoio[3,4-d]pyrimidin-4-one but substituting 5-amino-1 -cyclopentyl-1 H-pyrazole-4-5 carboxamide and (3,4-trans)-methyl-1-benzyl-4-cyclopropylpyrrolidine-3-carboxylate provided the title compound. 400 MHz 1H NMR (CDCI3) 6 7.99 (s, 1H), 7.38-7.31 (m, 4H), 7.27-7.23 (m, 1H), 5.14-5.10 (m, 1H), 3.78 (d, J = 12.4 Hz, 1H), 3.63 (d, J = 12.4 Hz, 1H), 3.32 (t, J = 8.7 Hz, 1H), 3.12-3.10 (m, 1H), 3.01 (d, J = 9.9 Hz, 1H), 2.59-2.57 (m, 1H), 2.20-1.87 (m, 8H), 10 1.71-1.65 (m, 2H), 0.86-0.84 (m, 1H), 0.52-0.48 (m, 2H), 0.19-0.09 (m, 2H). MS: (M+H m/z = 393.1). isopropylhydrazine provided the title compound. 400 MHz 1H NMR (CDCI3) 5 7.50 (s, 1H), 4.22-4.16 (m, 3H), 1.45 (d, J = 6.6 Hz, 6H). MS: (M+H m/z = 151.1). 20 (b) 5-amino-1 -isopropyl-1 H-pyrazole-4-carboxamide Following the procedure for the preparation of 5-amino-1-(2-methoxyphenyl)-1 H-pyrazole-4-carboxamide but substituting 5-amino-1- EXAMPLE 30 (a) 5-amino-1-isopropyl-1 H-pvrazole-4-carbonitrile 15 Following the procedure for the preparation of 5-amino-1-(2-methoxyphenyl)-1 H-pyrazole-4-carbonitrile but substituting 1- WO 2008/139293 PCT/IB2008/001125 -70- isopropyl-1 H-pyrazole-4-carbonitrile provided the title compound. 400 MHz 1H NMR (CDCI3) 6 7.67 (s, 1H), 4.40-4.33 (m, 1H), 1.37 (d, J = 6.6 Hz, 6H). MS: (M+H m/z = 169.1). (c) 6-f(3.4-trans)-1-benzvl-4-methvlpyrrolidin-3-vn-1-isopropvl-1.5-dihvdro-4H-5 pvrazolof3.4-dlpyrimidin-4-one Following the procedure for the preparation of 6-[(3,4-trans)-1-benzyl-4-methylpyrrolidin-3-yl]-1-(2-methoxyphenyl)-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one but substituting 5-amino-1 -isopropyl-1 H-pyrazole-4-10 carboxamide provided the title compound. 400 MHz 1H NMR (CDCI3) 5 7.98 (s, 1H), 7.38-7.22 (m, 5H), 5.07-5.04 (m, 1H), 3.77-3.64 (m, 2H), 3.12-3.08 (m, 1H), 2.99-2.91 (m, 3H), 2.67-2.63 (m, 1H), 2.27-2.25 (m, 1H), 1.48 <d, J = 7.1 Hz, 6H), 1.14 (d, J = 6.6 Hz, 3H). MS: (M+H m/z = 352.1). 15 6-f(3S.4S)-1-benzvl-4-methvlpvrrolidin-3-vl]-1-isopropvl-1,5-dihvdro-4H-pvrazolof3.4-dlpyrimidin-4-one The racemate was separated on Chiralcel OD chiral HPLC column, Mobile Phase 90/10 Heptane/EtOH, TR = 8.917, to provide the enantiomer. 20 400 MHz 1H NMR (CD3OD) 5 7.98 (s, 1H), 7.38-7.33 (m, 2H), 7.33-7.29 <m, 2H), 7.26-7.22 (m, 1H), 5.07-5.02 (m, 1H), 3.77-3.64 (m, 2H), 3.12-3.07 (m, 1H), 2.99-2.91 (m, 3H), 2.67-2.63 (m, 1H), 2.29-2.25 (m, 1H), 1,48(d, J = 6.6 Hz, 6H), 1.14 (d, J = 6.6 Hz, 3H). MS: (M+H m/z 352.1). EXAMPLE 31 WO 2008/139293 PCT/IB2008/001125 -71- EXAMPLE 32 (a) 1-isopropvl-6-f(3.4-trans)-4-methylpyrrolidin-3-yll-1 H-pyrazolor3.4- dlpyrimidin-4(5H)-one 0 5 Following the procedure for the preparation of 1-cyclopentyl-6-[(3S,4S)- 4-methylpyrrolidin-3-yl]-1 H-pyrazolc[3,4-d]pyrimidin-4(5H)-one but substituting the 6-[(3,4-trans)-1-benzyl-4-methylpyrrolidin-3-yl]-1 -isopropyl-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one provided the title compound. 400 MHz 1H NMR (CD3OD) 5 8.02 (s, 1H), 5.11-5.08 (m, 1H), 3.80-3.74 (m, 2H), 3.64-10 3.57 (m,1H), 3.30-3.22 (m, 1H), 3.07-3.02 (m, 1H), 2.75-2.71 (m, 1H), 1.49 (dd, J = 6.6, 1.7 Hz, 6H), 1.15 (d, J = 7.1 Hz, 3H). MS: (M+H m/z = 262.2). (b) 1-isopropvl-6-f(3.4-trans)-4-methvl-1-(auinolin-2-vlmethvDpvrrolidin-3-vn-1.5-dihvdro-4H-pyrazolof3.4-dlpvrimidin-4-one trans)-4-methyl-1-(pyridin-3-ylmethyl)pyrrolidin-3-yl]-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one but substituting 1-isopropyl-6-[(3,4-trans)-4-methylpyrrolidin-3-yl]-1 H-pyrazolo[3,4-d]pyrimidin-4(5H)-one and quinoline-2-carbaldehyde provided the title compound. 400 MHz 1H NMR (CD3OD) 5 20 8.31 (d, J = 8.7 Hz, 1H), 8.02 (d, J = 8.7 Hz, 1H), 7.98 (s, 1H), 7.95 (d, J = 7.9 Hz, 1H), 7.75-7.68 (m, 2H), 7.58-7.54 (m, 1H), 5.07-5.04 (m, 1H), 4.11-3.96 (m, 2H), 3.22-3.18 (m, 1H), 3.14-3.11 (m, 1H), 3.05-2.96 (m, 2H), (Racl 15 Following the procedure for the preparation of 1-cyclopentyl-6-[(3,4- WO 2008/139293 PCT/IB2008/001125 -72- 2.71-2.68 (m, 1H), 2.42-2.37 (m, 1H), 1.49 (q, J = 6.6 Hz, 6H), 1.17 (d, J = 7.1 Hz, 3H). MS: (M+H m/z 403.1). EXAMPLE 33 1-isopropvl-6-r(3.4-trans)-4-methvl-1-fQuinoxalin-6-vlmethvl)py[Tolidin-3-vn-5 1.5-dihvdro-4H-pvrazolor3.4-dlpvrimidin~4-one [Rac] trans)-4-methyl-1-(pyridin-3-ylmethyl)pyrrolidin-3-yl]-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one but substituting 1-isopropyl-6-[(3,4-trans)-4-10 methylpyrrolidin-3-yl]-1 H-pyrazolo[3,4-d]pyrimidin-4(5H)-one and quinoxaline-6-carbaldehydeprovided the title compound. 400 MHz 1H NMR (CD3OD) 5 8.84-8.81 (m, 2H), 8.04-8.00 (m, 2H), 7.96 (s, 1H), 7.91-7.88 (m, 1H), 5.09-5.02 (m, 1H), 4.01-3.87 (m, 2H), 3.14-3.93 (m, 4H), 2.72-2.66 (m, 1H), 2.39-2.34 (m, 1H), 1.47 (dd, J = 6.6, 1.2 Hz, 6H), 1.15 (d, J = 6.6 Hz, 3H). MS: 15 (M+H m/z 404.1). EXAMPLE 34 1-isoDroDvl-6-[(3.4-trans)-4-methvl-1-(auinolin-3-vlmethvl)Dvrrolidin-3-vll-1.5-dihvdro-4H-pvrazolor3.4-dlpyrimidin-4-one [Rac] o WO 2008/139293 PCT/IB2008/001125 -73- Following the procedure for the preparation of 1-cyciopentyl-6-[(3,4-trans)-4-methyl-1-(pyridin-3-ylmethyl)pyrrolidin-3-yl]-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one but substituting 1-isopropyl-6-[(3,4-trans)-4-methylpyrrolidin-3-yl]-1 H-pyrazolo[3,4-d]pyrimidin-4(5H)-one and quinoline-3-5 carbaidehyde provided the title compound. 400 MHz 1H NMR (CD3OD) 6 8.87 (d, J = 2.1 Hz, 1H), 8.30 (d, J = 1.2 Hz, 1H), 7.99 (d, J = 8.7 Hz, 1H), 7.97 (s, 1H), 7.90 (d, J = 7.5 Hz, 1H), 7.74-7.69 (m, 1H), 7.60-7.56 (m, 1H), 5.06-4.99 (m, 1H), 3.97-3.87 (m, 2H), 3.13-3.09 (m, 1H), 3.05-3.02 (m, 2H), 2.97-2.93 (m, 1H), 2.73-2.66 (m, 1H), 2.39-2.35 (m, 1H), 1.46 (dd, J = 6.6, 10 2.3 Hz, 6H), 1.15 (d, J = 7.1 Hz, 3H). MS: (M+H m/z 403.1). (a) f3.4-trans)-ethvl-1-benzvl-4-(trifluoromethvhPvrrolidine-3-carboxvlate 15 benzyl-4-methylpyrrolidine-3-carboxylate but substituting (E)-methyl 4,4,4-trifluorobut-2-enoate provided the title compound. 400 MHz 1H NMR (CDCI3) 5 7.33-7.22 (m, 5H), 4.16 (q, J = 7.1 Hz, 2H), 3.65-3.56 (m, 2H), 3.40-3.32 (m, 1H), 3.12-3.07 (m, 1H), 2.90-2.76 (m, 3H), 2.70-2.66 (m, 1H), 1.24 (t, J = 7.1 Hz, 3H). MS: (M+H m/z = 302.1). 20 (b) 6-r(3.4-trans)-1-benzvl-4-(trifluoromethvl)Dvrrolidin-3-vn-1-cvclopentvl-1.5-dihvdro-4H-pvrazolof3.4-dlpyrimidin-4-one EXAMPLE 35 Following the procedure for the preparation of (3,4-trans)-methyl 1- WO 2008/139293 PCT/IB2008/001125 -74- Following the procedure for the preparation of 6-[(3,4-trans)-1-benzyl-4-methylpyrrolidin-3-yl]-1-(2-methoxyphenyl)-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one but substituting 5-amino-1 -cyclopentyl-1 H-pyrazole-4-carboxamide and (3,4-trans)-methyl 1-benzyl-4-(trifluoromethyl)pyrrolidine-3-5 carboxylate provided the title compound. 400 MHz 1H NMR (CDCI3) 8 8.02 (s, 1H), 7.38-7.26 (m, 5H), 5.15-5.11 (m, 1H), 3.83 (d, J = 12.5 Hz, 1H), 3.64 (d, J = 12.5 Hz, 1H), 3.44-3.41 (m, 1H), 3.34 (t, J= 9.1 Hz, 1H), 3.12-3.10 (m, 1H), 3.03 (d, J = 9.9 Hz, 1H), 2.70-2.65 (m, 1H), 2.55-5.50 (m, 1H), 2.11-1.93 (m, 4H), 1.72-1.66 (m, 2H), 0.86-0.83 (m, 2H). MS: (M+H m/z = 10 432.0). EXAMPLE 36 (a) 1-isopropvl-6-r(3S.4S)-4-methvlpvrrolidin-3-vl1-1H-pvrazolor3.4- dlDvrimidin-4(5H) one Following the procedure for the preparation of 1-cyclopentyl-6-[(3S,4S)-20 4-methylpyrrolidin-3-yl]-1 H-pyrazolo[3,4-d]pyrimidin-4(5H)-one but substituting the 6-[(3S,4S)-1 -benzyl-4-methylpyrrolidin-3-yl]-1-isopropyl-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one provided the title compound. 400 MHz 1H NMR (CD3OD) 5 8.02 (s, 1H), 5.11-5.08 (m, 1H), 3.80-3.74 (m, 2H), 3.64-3.57 (m, 1H), 3.30-3.22 (m, 1H), 3.07-3.02 (m, 1H), 2.75-2.71 (m, 1H), 1.49 (dd, J = 25 6.6, 1.7 Hz, 6H), 1.15 (d, J = 7.1 Hz, 3H). MS: (M+H m/z = 262.2). (b) 1-isopropyl-6-f/3S.4S)-4-methyl-1-(quinoxalin-6-vlmethvl)pvrrolidin-3-yll-1.5-dihvdro-4H-pvrazolor3.4-dlpyrimidin-4-one (Abs] WO 2008/139293 PCT/IB2008/001125 -75- Following the procedure for the preparation of 1-cyclopentyl-6-[(3,4-trans)-4-methyl-1-(pyridin-3-yimethyl)pyrrolidin-3-yl]-1,5-dihydro-4H-pyrazoio[3,4-d]pyrimidin-4-one but substituting 1-isopropyl-6-[(3S,4S)-4-methylpyrrolidin-3-yl]-1 H-pyrazolo[3,4-d]pyrimidin-4(5H)-one and quinoxaline-5 6-carbaldehyde provided the title compound. 400 MHz 1H NMR (CD3OD) 5 11.0 (brs, 1H), 8.81 (s, 2H), 8.13 (d, J = 8.7, 1H), 8.01-7.92 (m, 3H), 5.01-4.94 (m,1H), 4.01-3.88 (m, 2H), 3.37 (t, J = 8.3, 1H), 3.05 (d , J = 9.9, 1H), 2.86-2.85 (m, 1H), 2.70-2.68 (m, 1H), 2.49-2.44 (m, 1H), 2.07-2.01 (m, 1H), 1.48 (dd, J = 15.3, 6.6 Hz, 6H), 1.20 (d, J = 7.05 Hz, 3H). MS: (M+H m/z 10 404.1). EXAMPLE 37 1-isoDropyl-6-rf3S.4S)-4-methvl-1-(Quinolin-3-vlmethvl)pyrrolidin-3-vn-1.5-dihvdro-4H-pvrazolor3.4-dlovrimidin-4-one l-cvclopentvl-e-fPS^SM-methvl-l-rfS-methvlpyr vlV1.5-dihvdro-4H-pyrazolo[3.4-dlpvrimidin-4-one WO 2008/139293 PCT/IB2008/001125 -76- -O Following the procedure for the preparation of 1-cyclopentyl-6-[(3,4-trans)-4-methyl-1-(pyridin-3-ylmethyl)pyrrolidin-3-yl]-1,5-dihydro-4H-5 pyrazolo[3,4-d]pyrimidin-4-one but substituting 1-cyclopentyl-6-[(3S,4S)-4- methylpyrazine-2-carbaldehyde provided the title compound. 400 MHz 1H NMR (CDCI3) 5 8.53 (s, 1H), 8.53 (s, 1H), 8.01 (s, 1H), 5.16-5.05 (m, 1H), 4.02 (d, J = 14.1 Hz, 1H), 3.74 (d, J = 14.1 Hz, 1H), 3.45-3.41 (m, 1H), 3.38 10 (t, J = 8.3 Hz, 1H), 3.08 (d, J = 9.9 Hz, 1H); 2.84-2.83 (m, 1H), 2.62-2.54 (m, 1H), 2.52 (s, 3H), 2.47-2.40 (m, 1H), 2.14-1.89 (m, 6H), 1.71-1.64 (m, 2H), 1.24-1.16 (m, 3H). MS: (M+H m/z = 394.1). 6-f(3.4-trans)-1-benzvl-4-ethvlpvrrolidin-3-vll-1-isopropy|-1.5-dihvdro-4H-15 Dvrazolof3.4-dlpyrimidin-4-one Following the procedure for the preparation of 6-[(3,4-trans)-1-benzyl-4-methylpyrrolidin-3-yl]-1-(2-methoxyphenyl)-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one but substituting 5-amino-1-isopropyl-1 H-pyrazole-4-20 carboxamideand (3,4-trans)-methyl 1-benzyl-4-ethylpyrrolidine-3-carboxylate provided the title compound. 400 MHz 1H NMR (CD3OD) 5 8.01 (s, 1H), 7.38-7.22 (m, 5H), 5.06-5.01 (m, 1H), 3.76-3.64 (m, 2H), 3.12 (t, J = 8.71 Hz, 1H), 3.00-2.91 (m,2H), 2.87-2.82 (m, 1H), 2.51-2.47 (m, 1H), 2.30-2.26 (m, methylpyrrolidin-3-yl]-1 H-pyrazolo[3,4-d]pyrimidin-4(5H)-one and 5- EXAMPLE 39 [Rac] WO 2008/139293 PCT/IB2008/001125 -77- 1H), 1.60-1.50 (m, 2H), 1.49 (d, J = 7.05 Hz, 6H), 3.08 (d, J = 7.5 Hz, 3H). MS: (M+H m/z = 366.1). EXAMPLE 40 6-rf3S.4S)-1-benzvl-4-ethvlpvrrolidin-3-vll-1-isopropyl-1.5-dihvdro-4H-5 Dvrazolor3.4-d1pvrimidin-4-one [Abs] The racemate was separated on Chiralcel OJ chiral HPLC column, Mobile Phase 80/20 Heptane/EtOH, TR = 9.177, to provide the enantiomer. 400 MHz 1H NMR (CD3OD) 5 8.01 (s, 1H), 7.38-7.22 (m, 5H), 5.06-5.01 (m, 10 1H), 3.76-3.64 (m, 2H), 3.12 (t, J = 8.71 Hz, 1H), 3.00-2.91 (m, 2H), 2.87-2.82 (m,1H), 2.51-2.47 (m, 1H), 2.30-2.26 (m, 1H), 1.60-1.50 (m, 2H), 1.49 (d, J = 7.05 Hz, 6H), 3.08 (d, J = 7.5 Hz, 3H). MS: (M+H m/z = 366.2). EXAMPLE 41 1-isopropvl-6-((,3S.4SM-methvl-1-IY2-methvlpvrimidin-5-vl)methvnpyrrolidin-3-15 vIM .5-dihvdro-4H-pvrazolo[3.4-dlpyrimidin-4-one ■AbsJ Following the procedure for the preparation of 1-cyclopentyl-6-[(3,4-trans)-4-methyl-1-(pyridin-3-ylmethyl)pyrrolidin-3-yi]-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one but substituting 1-isopropyl-6-[(3S,4S)-4-20 methylpyrrolidin-3-yl]-1H-pyrazolo[3,4-d]pyrimidin-4(5H)-one and 2-methyipyrimidine-5-carbaldehyde provided the title compound. 400 MHz 1H NMR (CD3OD) 5 8.68 (s, 2H), 7.97 (s,1H), 5.08-5.01 (m,1H), 3.78-3.68 {m, WO 2008/139293 PCT/IB2008/001125 -78- 2H), 3.08-2.92 (m, 4H), 2.74-2.67 (m, 1H), 2.65 (s, 3H), 2.36-2.32 (m, 1H), 1.48 (d, J =6.6 Hz, 6H), 1.15 (d, J = 7.1 Hz, 3H). MS: (M+H m/z = 368.1). EXAMPLE 42 6-M-benzvlpvrrolidin-3-vl)-1-cvclopentvl-1.5-dihvdro-4H-pyrazolof3.4-5 dlpvrimidin-4-one 0 Following the procedure for the preparation of 6-[(3,4-trans)-1-benzyl-4-methylpyrrolidin-3-yl]-1-(2-methoxyphenyl)-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one but substituting 5-amino-1 -cyclopentyl-1 H-pyrazole-4-10 carboxamide and methyl 1-benzylpyrroiidine-3-carboxylate provided the title compound. 400 MHz 1H NMR (CDCI3) 5 8.01 (s, 1H), 7.39-7.32 (m, 4H), 7.27-7.23 (m, 1H), 5.12-5.09 (m, 1H), 3.84 (d, J =12.4 Hz, 1H), 3.61 (d, J = 12.4 Hz, 1H), 3.30-3.26 (m, 1H), 3.18-3.14 (m, 1H), 3.01 (d, J = 9.9 Hz, 1H), 2.45-2.32 (m, 3H), 2.11-1.90 (m, 7H), 1.71-1.65 (m, 2H). MS: (M+H 15 m/z = 364.1). EXAMPLE 43 1-isopropvl-6-((3S,4S)-1-[(6-methoxvPvridin-3-vl)methvn-4-methvlpyrrolidin-3-vl)-1.5-dihvdro-4H-pyrazolor3.4-dlpyrimidin-4-one (abs) / 20 Following the procedure for the preparation of 1-cyclopentyl-6-[(3,4- trans)-4-methyl-1-(pyridin-3-ylmethyl)pyrrolidin-3-yl]-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one but substituting 1-isopropyl-6-[(3S,4S)-4-methylpyrrolidin-3-yl]-1 H-pyrazolo[3,4-d]pyrimidin-4(5H)-one and 6- WO 2008/139293 PCT/IB2008/001125 -79- methoxynicotinaldehyde provided the title compound. 400 MHz 1H NMR (CDCIs) 6 8.02 (s, 2H), 7.72 (m, 1H), 6.77 (d, J = 8.3 Hz, 1H), 3.90 <s, 3H), 3.73-3.53 (m, 2H), 3.39-3.33 (m, 1H), 3.30 (m, 1H), 2.8 (m, 1H), 2.55-2.40 (m, 1H), 1.9 (m, 1H), 1.57-1.53 (m, 2H), 1.52-1.47 (m, 6H), 1.19 (d, J = 6.6 Hz, 5 3H). MS: (M*H m/z = 383.2). EXAMPLE 44 (a) 6-((3,4-trans)-4-ethylpvrrolidin-3-vl)-1-isopropyl-1 H-Pvrazolof3.4- dlpyrimidin-4(5H)-one 10 Following the procedure for the preparation of 1-cyclopenty!-6-[(3S,4S)- 4-methylpyrrolidin-3-yl]-1 H-pyrazolo[3,4-d]pyrimidin-4(5H)-one but substituting the 6-[(3,4-trans)-1 -benzyl-4-ethylpyrrolid in-3-yi]-1 -isopropyl-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one provided the title compound. 400 MHz 1H NMR (CD3OD) 5 8.02 (s, 1H), 5.10 (m, 1H), 3.78-3.60 (m, 3H), 3.32-3.28 15 (m, 1H), 3.10 (m,1H), 2.62 (m,1H), 1.65 (m,1H), 1.58 (m,1H), 1.49 (dd, J = 6.6, 1.7 Hz, 6H), 0.97 (t, J = 7.5 Hz, 3H). MS: (M+H m/z = 276.1). (b) 6-f(3.4-trans)-4-ethvl-1-(auinolin-3-vlmethvl)pvrrolidin-3-yll-1-isopropyl-1.5-dihvdro-4H-pvrazolor3.4-dlpyrimidin-4-one (Rac) trans)-4-methyl-1-(pyridin-3-ylmethyl)pyrrolidin-3-yl]-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one but substituting 6-((3,4-trans)-4-ethyipyrrolidin-3-yl)-1 -isopropyl-1 H-pyrazolo[3,4-d]pyrimidin-4(5H)-one and quinoline-3- WO 2008/139293 PCT/IB2008/001125 -80- carbaldehyde provided the title compound. 400 MHz 1H NMR (CD3OD) 6 8.86 (d, J = 2.1 Hz, 1H), 8.29 (d, J = 1.7 Hz, 1H), 7.98 (d, J = 8.3 Hz, 1H), 7.97 (s, 1H), 7.89 (d, J= 8.3 Hz, 1H), 7.73-7.68 (m, 1 H), 7.59-7.55 (m, 1H), 5.05-4.98 (m, 1H), 3.94-3.85 (m, 2H), 3.13-3.09 (m, 1H), 3.02-2.94 (m, 3H), 5 2.57-2.52 (m, 1H), 2.39-2.34 (m, 1H), 1.58-1.48 <m, 2H), 1.48 (d, J = 6.6 Hz, 6H) 10.87 (t, J = 7.05 Hz, 3H). MS: (M+H m/z = 417.0). EXAMPLE 45 6-((3.4-trans)-4-ethvl-1-r(6-methoxvpyridin-3-yl)methyllpyrrolidin-3-vl}-1-isopropyl-1.5-dihvdro-4H-pvrazolof3.4-dlpyrimidin-4-one (Racj trans)-4-methyl-1-(pyridin-3-ylmethyl)pyrrolidin-3-yl]-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one but substituting 6-((3,4-trans)-4-ethylpyrrolidin-3-yl)-1 -isopropyl-1 H-pyrazolo[3,4-d]pyrimidin-4(5H)-one and 6- 15 methoxynicotinaldehyde provided the title compound. 400 MHz 1H NMR (CDCI3) 5 10.83 (brs, 1H), 8.02 (d, J = 2.1 Hz, 1H), 8.00 (s, 1H), 7.69 (dd, J = 8.7, 2.1 Hz, 1H), 6.77 (d, J = 8.3 Hz, 1H), 4.98-4.94 (m, 1H), 3.89 (s, 3H), 3.69-3.54 (m, 2H), 3.29 (t, J = 8.5 Hz, 1H), 2.98 (d, J = 9.9 Hz, 1H), 2.88-2.87 (m,1H), 2.55 (m,1H), 2.18 (m, 1H), 1.91 (m, 1H), 1.60-1.55 (m, 1H), 20 1.51-1.47 (m, 7H), 0.91 (t, J = 7.5 Hz, 3H). MS: (M+H m/z = 397.0). EXAMPLE 46 6-rf3.4-trans)-4-ethvl-1-(quinoxalin-6-vlmethvl)pvrrolidin-3-vl]-1-isopropyl-1.5-dihvdro-4H-pvrazolof3.4-dlpyrimidin-4-one WO 2008/139293 PCT/IB2008/001125 -81- V-/ Following the procedure for the preparation of 1-cyclopentyl-6-[(3,4-trans)-4-methyl-1 -(pyridin-3-ylmethyl )pyrrolidin-3-yl]-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one but substituting 6-((3,4-trans)-4-ethylpyrrolidin-5 3-y!)-l-isopropyl-1 H-pyrazolo[3,4-d]pyrimidin-4(5H)-one and quinoxaline-6-carbaldehyde provided the title compound. 400 MHz 1H NMR (CDCb) 6 10.83 (brs, 1H), 8.81 (s, 2H), 7.79 (d, J = 2.5 Hz, 1H), 8.15-7.92 (m, 3H), 4.99-4.92 (m, 1H), 3.98-3.86 (m, 2H), 3.35 (t, J = 8.7 Hz, 1H), 3.04 (d, J = 9.9 Hz, 1H), 2.92-2.91 (m, 1H), 2.61-2.57 (m, 1H), 2.25-2.2 (m, 1H), 2.02 (t, J = 8.7 Hz, 10 1H), 1.64-1.52 (m, 1H), 1.50-1.45 (m, 7H), 0.91 (t, J = 7.5 Hz, 3H). MS: (M+H m/z = 418.1). Following the procedure for the preparation of 1-cyclopentyl-6-[(3,4-trans)-4-methyl-1-(pyridin-3-ylmethyl)pyrrolidin-3-yl]-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one but substituting 1-cyclopentyl-6-((3S,4S)-4-methylpyrrolidin-3-yl]-1 H-pyrazolo[3,4-d]pyrimidin-4(5H)-one and 1,3-20 dimethyl-1H-pyrazole-5-carbaldehyde provided the title compound. 400 MHz 1H NMR (CDCI3) 5 7.99 (s, 1H), 5.95-5.94 (m, 1H), 5.14-5.09 (m, 1H), 3.87 (s, 3H), 3.81 (s, 1 H), 3.66 (q, J= 14.5 Hz, 2H), 3.33 (t, J = 8.3 Hz, 1H), 3.03 EXAMPLE 47 1-cvclopentvl-6-((3S.4S)-1-IY1.3-dimethy[-1H-pyrazol-5-v[)methvl1-4-methvlPvrrolidin-3-vl)-1.5-dihvdro-4H-pyrazolor3.4-dlpvrimidin-4-one Cajos) 15 WO 2008/139293 PCT/IB2008/001125 -82- (d, J = 9.9 Hz, 1H), 2.84-2.82 (m, 1H), 2.59-2.55 (m, 1H), 2.44-2.41 (m, 1H), 2.20-2.19 (m, 3H), 2.12-1.90 (m, 5H), 1.70-1.65 (m, 2H), 1.25-1.18 (m, 3H). MS: (M+H m/z = 396.1). EXAMPLE 48 5 1-cvclopentvl-6-r(3S.4S)-4-methvl-1-(4.5.6.7-tetrahvdropvrazolon.5-alpyridin-3-vlmethvD pvrrolidin-3-vll-1.5-dihvdro-4H-pvrazolor3.4-dlpyrimidin-4-one [Absl trans)-4-methyl-1-(pyridin-3-ylmethyl)pyrrolidin-3-yl]-1,5-dihydro-4H-10 pyrazolo[3,4-d]pyrimidin-4-one but substituting 1-cyclopentyi-6-[(3S,4S)-4-methylpyrrolidin-3-yl]-1 H-pyrazolo[3,4-d]pyrimidin-4(5H)-one and 4,5,6,7-tetrahydropyrazolo[1,5-a]pyridine-3-carbaldehyde provided the title compound. 400 MHz 1H NMR (CDCI3) 5 7.99 (s, 1H), 7.35 (s, 1H), 5.16-5.09 (m, 1H), 4.11-4.06 (m, 2H), 3.68 (d, J = 13.3 Hz, 1H), 3.57 (d, J = 13.3 Hz, 15 1H), 3.32 (t, J = 8.7 Hz, 1H), 3.00 (d, J = 9.9 Hz, 1H), 2.94-2.80 (m, 2H), 2.78-2.77 (m, 1H), 2.49-2.45 (m, 1H), 2.38-2.33 (m, 1H), 2.11-1.83 (m, 11H), 1.73-1.61 (m,2H), 1.17 (d, J = 7.1 Hz, 3H). MS: (M+H m/z = 422.1). EXAMPLE 49 1-cvclopentvl-6-(f3S.4S)-4-methvl-1-f(1-methvl-1H-benzimidazol-2-20 vl)methvllPvrrolidin-3-vl>-1.5-dihvdro-4H-pyrazolof3.4-dlpvrimidin-4-one WO 2008/139293 PCT/IB2008/001125 -83- Following the procedure for the preparation of 1-cyclopentyl-6-[(3,4-trans)-4-methyl-1 -(pyridin-3-ylmethyl)pyrrolidin-3-yl]-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one but substituting 1-cyclopentyl-6-[(3S,4S)-4-methylpyrrolidin-3-yl]-1 H-pyrazolo[3,4-d]pyrimidin-4(5H)-one and 1 -methyl-5 1H-benzo[d]imidazole-2-carbaldehyde provided the title compound. 400 MHz 1H NMR (CDCI3) 5 7.98 (s, 1H), 7.73 (m, 1H), 7.35 (dd, J = 7.1, 1.2 Hz, 1H), 7.29-7.21 (m, 2H), 5.13-5.09 (m, 1H), 4.11-4.06 (m, 2H), 3.97 (s, 3H), 3.37 (t, J = 8.3 Hz, 1H), 3.06 (d, J = 10.4 Hz, 1H), 2.88-2.85 (m, 1H), 2.77-2.73 (m, 1H), 2.16-1.92 (m, 8H), 1.70-1.66 (m, 2H), 1.21 (d, J =7.1 Hz, 3H). MS: 10 (M+H m/z = 432.1). EXAMPLE 50 1-isopropvl-6-(f3S.4S)-4-methyl-1-f(5-methvlpvrazin-2-vl)methvnpvrrolidin-3-vD-1.5-dihvdro-4H-pvrazolof3.4-dlPvrimidin-4-one [Absl 0 15 Following the procedure for the preparation of 1-cyclopentyl-6-{(3,4- trans)-4-methyl-1-(pyridin-3-ylmethyl)pyrrolidin-3-yl]-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one but substituting 1-isopropyl-6-[(3S,4S)-4-methylpyrrolidin-3-yl]-1 H-pyrazolo[3,4-d]pyrimidin-4(5H)-one and 5-methylpyrazine-2-carbaldehyde provided the title compound. 400 MHz 1H 20 NMR (CDCI3) 5 11.4 (brs, 1H), 8.49 (s,1H), 8.44 (s,1H), 7.99 (s,1H), 5.00-4.93 (m,1H), 4.00-3.97 (m, 1H), 3.78-3.67 (m, 1H), 3.33 (t, J = 8.3 Hz, 1H), 3.04 (d, J = 9.9 Hz, 1H), 2.85-2.82 (m, 1H), 2.65-2.61 (m, 1H), 2.49 (s, 3H), 2.48-2.41 (m, 1H), 2.13 (t, J = 8.5 Hz, 1H), 1.46 (dd, J = 11.6,6.6 Hz, 6H), 1.17 (d, J= 7.05 Hz, 3H). MS: (M+H m/z = 368.1). 25 EXAMPLE 51 6-ff3S.4S)-1-(cinnolin-3-vlmethvl)-4-methvlpyrrolidin-3-vll-1-cvclopentvl-1.5- dihvdro-4H-pvrazolor3.4-dlpyrimidin-4-one WO 2008/139293 PCT/IB2008/001125 -84- Following the procedure for the preparation of 1-cyclopentyl-6-[(3,4-trans)-4-methyl-1-(pyridin-3-ylmethyl)pyrrolidin-3-yl]-1,5-dihydro-4H-pyrazoio[3,4-d]pyrimidin-4-one but substituting 1-cyclopentyl-6-{(3S,4S)-4-5 methylpyrrolidin-3-yl]-1H-pyrazolo[3,4-d]pyrimidin-4(5H)-one and cinnoline-3-carbaldehyde provided the title compound. 400 MHz 1H NMR (CDCI3) 5 8.49 (d, J = 8.7 Hz, 1H), 8.16 (s, 1H), 8.01 (s, 1H), 7.93 (d, J = 7.9 Hz, 1H), 7.83-7.81 (m, 1H), 7.79-7.71 (m, 1H), 5.16-5.09 (m, 1H), 4.47-4.44 (m, 1H), 4.35-4.32 (m, 1H), 3.49-3.45 (m, 2H), 3.10-3.07 (m, 1H), 2.87-2.71 (m, 2H), 10 2.49-2.48 (m, 1H), 2.23 (m,1H), 2.11-1.87 (m, 5H), 1.73-1.63 (m, 2H), 1.22 (d, J= 7.1 Hz, 3H). MS: (M+H m/z = 430.1). Following the procedure for the preparation of 1-cyclopentyl-6-[(3S,4S)-4-methylpyrrolidin-3-yl]-1 H-pyrazolo[3,4-d]pyrimidin-4<5H)-one but substituting the 6-[(3,4-trans)-1 -benzyl-4-(trifluoromethyl)pyrrolidin-3-yl]-1 -cyclopentyl-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one provided the title compound. 400 20 MHz 1H NMR (CD3OD) 5 7.98 (s, 1H), 5.23 (m, 1H), 3.73 (m,1H), 3.42 (m, 2H), 3.10 (m,2H), 2.19-1.92 (m, 7H), 1.75 (m,2H), 1.23-1.19 (m, 3H). EXAMPLE 52 (a) 1-cvclopentvl-6-f(3.4-trans)-4-(trifluoromethvl)pvrrolidin-3-vn-1H- Dvrazolof3.4-dlpvrimidin-4f5H)-one 15 WO 2008/139293 PCT/IB2008/001125 -85- (b) 1-cyclopentvl-6-f(3.4-trans)-1-(Quinoxalin-6-ylmethyl)-4- ('trifluoromethyl)pyrrolidin-3-yl]-1.5-dihydro-4H-pyrazolof3.4-dlpyrimidin-4-one Following the procedure for the preparation of 1-cyclopentyl-6-[(3,4-5 trans)-4-methyl-1-(pyridin-3-ylmethyl)pyrrolidin-3-yl]-1,5-dihydro-4H- pyrazolo[3,4-d]pyrimidin-4-one but substituting 1-cyclopentyl-6-[(3,4-trans)-4-(trifiuoromethyl)pyrrolidin-3-yl]-1H-pyrazolo[3,4-d]pyrimidin-4(5H)-one and quinoxaline-6-carbaldehyde provided the title compound. 400 MHz 1H NMR (CDCIs) 5 8.80 (s, 1H), 8.08 (d, J = 8.3 Hz, 1H), 7.99 (m,1H), 7.86-7.83 (m, 10 1H), 5.13-5.10 (m, 1H), 4.88 (s, 1H), 3.96-3.88 (m, 2H), 3.50-3.35 (m, 2H), 3.17 (t, J = 9.9 Hz, 1H), 3.03-2.99 (m, 1H), 2.92-2.88 (m, 1H), 2.79-2.75 (m, 1H), 2.11-1.90 (m, 6H), 1.71-1.65 (m, 1H). EXAMPLE 53 1-cvclopentvl-6-((3S.4S)-4-methvl-1-f(2-methvlpvrimidin-4-yl)methyllpyrrolidin-15 3-vl\-1.5-dihvdro-4H-pvrazolof3.4-dlpyrimidin-4-one Following the procedure for the preparation of 1-cyclopentyl-6-[(3,4-trans)-4-methyl-1 -(pyridin-3-ylmethyl )pyrrolidi n-3-yl]-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one but substituting 1-cyclopentyl-6-[(3S,4S)-4-20 methylpyrrolidin-3-yl]-1H-pyrazolo[3,4-d]pyrimidin-4(5H)-one and 2-methylpyrimidine-4-carbaldehyde provided the title compound. 400 MHz 1H NMR (CDCI3) 6 8.60 (d, J = 5.4 Hz, 1 H), 8.03 (s,1H), 7.24 (s,1H), 5.18-5.10 (m, 1H), 3.97-3.94 (m, 1H), 3.72-3.68 (m, 1H), 3.40 (t, J = 8.3 Hz, 1H), 3.15 WO 2008/139293 PCT/IB2008/001125 -86- (d, J = 9.5 Hz, 1H), 2.89 (m, 1H), 2.73 (s, 3H), 2.68-2.63 (m, 1H), 2.53-2.47 (m, 1H), 2.15-1.89 (m, 7H), 1.74-1.65 (m, 2H), 1.21 (d, J = 7.1 Hz, 3H). MS: (M+H m/z = 394.1). EXAMPLE 54 5 1-cvclopentvl-6-f(3S.4S)-1-(r2-(dimethvlamino)pvrimidin-4-vnrnethylM-methvlpyrrolidin-3-vn-1.5-dihvdro-4H-pvrazolor3.4-dlpyrimidin-4-one (Absj Following the procedure for the preparation of 1-cyclopentyl-6-[(3,4-trans)-4-methyl-1-(pyridin-3-ylmethyl)pyrrolidin-3-yl]-1,5-dihydro-4H-10 pyrazolo[3,4-dJpyrimidin-4-one but substituting 1-cyclopentyl-6-[(3S,4S)-4-methyipyrrolidin-3-yl]-1 H-pyrazolo[3,4-d]pyrimidin-4(5H)-one and 2-(dimethylamino)pyrimidine-4-carbaldehydeprovided the title compound. 400 MHz 1H NMR (CDCb) 6 8.24-8.21 (m, 1H), 8.00-7.99 (m, 1H), 6.52-6.50 (m, 1H), 5.13-5.10 (m, 1H), 3.72-3.68 (m, 1H), 3.56-3.52 (m, 1H), 3.46-3.40 (m, 15 3H), 3.17-3.11 (m, 7H), 2.84-2.82 (m, 1 H), 2.64-2.60 (m, 1H), 2.44-2.38 (m, 1H), 2.06-1.92 (m, 5H), 1.66-1.65 (m, 2H), 1.20-1.16 (m, 3H). MS: (M+H m/z = 423.1). EXAMPLE 55 (a) 1-cvclopentvl-6-f(3,4-trans)-4-cvclopropvlpyrrolidin-3-vn-1H-pyrazolof3.4-20 dlpyrimidin-4(5H)-one 1&> b Following the procedure for the preparation of 1-cyclopentyl-6-[(3S,4S)-4-methylpyrrolidin-3-yl]-1 H-pyrazolo[3,4-d]pyrimidin-4(5H)-one but substituting 6-[(3,4-trans)-1 -benzyl-4-cyclopropylpyrrolidin-3-yl]-1 -cyclopentyl-1,5-dihydro- WO 2008/139293 PCT/IB2008/001125 -87- 4H-pyrazolo[3,4-d]pyrimidin-4-one provided the title compound. 400 MHz 1H NMR (CDCI3) 5 8.08-7.94 (m, 1H), 5.17-5.10 (m, 1H), 3.39-3.34 (m, 1H), 3.29-3.28 (m, 2H), 3.17-3.14 (m, 1H), 2.13-1.91 (m, 7H), 1.78-1.67 (m, 3H), 0.82 (m,1H), 0.47-0.44 (m, 2H), 0.13-0.08 (m, 2H). MS: (M+H m/z = 314.2). (b) 1-cvcloDentvl-6-((3.4-trans)-4-cvcloproDvl-1-f(5-methvlDvrazin-2- vhmethvllPvrrolidin-3-vl}-1.5-dihvdro-4H-pvrazolof3.4-dlPvrimidin-4-one Following the procedure for the preparation of 1-cyclopentyl-6-[(3S,4S)-4-methylpyrrolidin-3-yl]-1 H-pyrazolo[3,4-d]pyrimidin-4(5H)-one but substituting 10 1-cyclopentyl-6-[(3,4-trans)-4-cyclopropylpyrrolidin-3-yl]-1 H-pyrazolo[3,4- d]pyrimidin-4(5H)-one and 5-methylpyrazine-2-carbaldehyde provided the title compound. 400 MHz 1H NMR (CDCI3) 5 8.52 (d, J = 0.8 Hz, 1H), 8.46 (s, 1H), 8.00 (s,1H), 5.14-5.10 (m, 1H), 4.04-4.01 (m, 1H), 3.78-3.75 (m, 1H), 3.35 (t, J = 8.7 Hz, 1H), 3.15-3.09 (m, 2H), 2.66-2.64 (m, 1H), 2.52 (s, 3H), 15 2.38 (t, J = 8.7 Hz, 1H), 2.11-2.01 (m, 3H), 1.96-1.91 (m, 2H), 1.73-1.65 (m, 2H), 1.24-1.20 (m, 1H), 0.59-0.83 (m, 2H), 0.53-0.49 (m, 2H), 0.23-0.11 (m, 2H). MS: (M+H m/z = 420.1). EXAMPLE 56 1-cvcloDentvl-6-f(3.4-trans)-4-cvclopropvl-1-(guinoxalin-6-vlmethvl)pvrrolidin-20 3-vl1-1.5-dihvdro-4H-pyrazolof3.4-dlpyrimidin-4-one Following the procedure for the preparation of 1-cyclopentyl-6-[(3S,4S)-4-methylpyrrolidin-3-yl]-1 H-pyrazolo[3,4-d]pyrimidin-4(5H)-one but substituting 1-cyclopentyl-6-[(3,4-trans)-4-cyclopropylpyrrolidin-3-yl]-1 H-pyrazolo[3,4- WO 2008/139293 PCT/IB2008/001125 -88- d]pyrimidin-4(5H)-one and quinoxaline-6-carbaldehyde provided the title compound. 400 MHz 1H NMR (CDCI3) 5 8.82 (s, 2H), 8.14 (d, J = 8.7 Hz, 1H), 8.00 (s, 2H), 7.96-7.94 (m, 1H), 5.16-5.09 (m, 1H), 3.98-3.95 (m, 2H), 3.35 (t, J = 8.3 Hz, 1H), 3.15-3.08 (m, 2H), 2.70 (m, 1H), 2.27 (m, 1H), 5 2.15-1.88 (m, 6H), 1.74-1.62 (m, 3H), 0.91-0.87 (m, 1H), 0.55-0.47 (m, 2H), 0.21-0.09 (m, 2H). MS: (M+H m/z = 456.1). 1-cvclopentvl-6-r(3.4-trans)-4-cvclopropyl-1-methvlpyrrolidin-3-vl]-1,5-dihvdro-4H-pvrazolof3.4-dlpyrimidin-4-one Following the procedure for the preparation of 1-cyclopentyl-6-[(3S,4S)-4-methylpyrrolidin-3-yl]-1 H-pyrazolo[3,4-d]pyrimidin-4(5H)-one but substituting 1-cyclopentyl-6-[(3,4-trans)-4-cyclopropylpyrrolidin-3-yl]-1 H-pyrazolo[3,4-d]pyrimidin-4(5H)-one and formaldehyde provided the title compound. 400 15 MHz 1H NMR (CDCI3) 5 8.01 (s, 1H), 5.19-5.11 (m, 1H), 3.31 (d, J = 8.7 Hz, 1H), 3.14-3.13 (m, 1H), 3.06 (d, J = 9.9 Hz, 1H), 2.57 (m,1H), 2.43 (s, 3H), 2.14-1.91 (m, 7H), 1.75-1.64 (m, 3H), 0.91-0.82 (m, 1H), 0.53-0.50 (m, 2H), 0.20-0.14 (m, 2H). MS: (M+H m/z = 328.2). 20 6-[f3.4-trans)-1 -benzvl-4-methvlpvrrolidin-3-vn-1 -ethvl-1,5-dihvdro-4H-pyrazolor3,4-d1pvrimidin-4-one Following the procedure for the preparation of 6-[(3,4-trans)-1-benzyl-4-methylpyrrolidin-3-yl]-1 -(2-methoxyphenyl )-1,5-dihydro-4H-pyrazolo[3,4- EXAMPLE 57 10 EXAMPLE 58 WO 2008/139293 PCT/IB2008/001125 -89- d]pyrimidin-4-one but substituting 5-amino-1-ethyl-1H-pyrazole-4-carboxamide provided the title compound. 400 MHz 1H NMR (CDCI3) 5 8.02 (s, 1H), 7.42-7.27 (m, 5H), 4.38-4.30 (m, 2H), 3.83-3.80 (m, 1 H), 3.63-3.59 (m, 1H), 3.49-3.35 (m, 1H), 2.99 (d, J = 10.3 Hz, 1H), 2.83-2.80 (m, 1H), 5 2.55-2.50 (m, 1H), 2.44-2.378 (m, 1H), 1.95-1.90 (m, 1H), 1.47 (t, J = 7.1 Hz, 3H), 1.22 (t, J= 10.3 Hz, 3H). MS: (M+H m/z = 338.1). (a) 6-((3S.4S)-4-ethvlpyrrolidin-3-vl)-1-isopropyl-1 H-ovrazolof3.4-dlpyrimidin-4(5m-one Following the procedure for the preparation of 1-cyclopentyl-6-[(3S,4S)-4-methylpyrrolidin-3-yl]-1 H-pyrazolo[3,4-d]pyrimidin-4(5H)-one but substituting 6-[(3S,4S)-1-benzyl-4-ethylpyrrolidin-3-yl]-1-isopropyl-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one provided the title compound. 400 MHz 1H 15 NMR (CD3OD) 5 8.02 (s, 1H), 5.10 (m,1H), 3.78-3.60 (m, 3H), 3.32-3.28 (m, 1H), 3.10 (m,1H), 2.62 (m,1H), 1.65 (m,1H), 1.58 (m,1H), 1.49 (dd, J = 6.6, 1.7 Hz, 6H), 0.97 (t, J = 7.5 Hz, 3H). MS: (M+H m/z = 276.1). (b) 6-((3.4-trans)-4-ethvl-1-f(2-methvlpyrimidin-5-vl)methvllpvrrolidin-3-vl}-1-isopropvl-1.5-dihvdro-4H-pvrazolor3,4-dlpyrimidin-4-one Following the procedure for the preparation of 1-cyclopentyl-6-[(3S,4S)-4-methylpyrrolidin-3-yl]-1 H-pyrazolo[3,4-d]pyrimidin-4(5H)-one but substituting 6-((3S,4S)-4-ethylpyrrolidin-3-yl)-1-isopropyl-1 H-pyrazolo[3,4-d]pyrimidin- EXAMPLE 59 10 20 WO 2008/139293 PCT/IB2008/001125 -90- 15 20 4(5H)-one and 2-methylpyrimidine-5-carbaldehyde provided the title compound. 400 MHz 1H NMR (CDCI3) 5 10.8 (brs, 1H), 8.62 (s, 2H), 8.01 (s, 1H), 5.00-4.93 (m, 1H), 3.66 (s, 2H), 3.25-3.20 (m, 1H), 3.02 (d, J = 9.9 Hz, 1H), 2.95-2.92 (m, 1H), 2.70 (s, 3H), 2.62-2.58 (m, 1H), 2.28-2.23 (m, 1H), 1.98 (t, J = 8.7 Hz, 1H), 1.62-1.52 (m, 1H), 1.48 (t, J = 6.6 Hz, 6H), 1.46-1.42 (m,1H), 3.08 (t, J = 7.5 Hz, 3H). MS: (M+H m/z = 382.2). EXAMPLE 60 (a) 1-(tetrahvdro-2H-pyran-4-vl)hvdrazine To a solution of tetrahydropyran-4-one (71.6 g, 715 mmol) in methanol (2 L) was added tert-butylcarbazate (100 g, 758 mmol) at ambient temp. The mixture was stirred at ambient temp for 20 h. The reaction mixture was concentrated under reduced pressure to dryness to afford a white solid (154 g). To a suspension of the white solid (154 g, 715 mmol) in water (1 L) was added acetic acid (500 mL, 8.73 mol) and the mixture was stirred for 30 min to get a clear solution. To this solution, solid NaCNBH3 (44.5 g, 708 mmol) was added portion-wise. The mixture was stirred at ambient temp for 2 h. The mixture was then transferred to a 12 L flask, cooled to 0 °C, and quenched with 1N NaOH (8.73 L, 8.73 mol). The mixture was extracted with CH2CI2 (3 x 3 L) and dried over Na2S04. The organic layer was filtered and concentrated to afford a white solid (164 g, contains -15% of N-acetyl-N'-Boc-hydrazine derivative). Chromatography [silica, ethyl acetate/MeOH (95:5] gave 94 g of 90% pure boc-hydrazine. A solution of boc-hydrazine (50 g, 231 mmol) in methanol (500 mL) was added a solution of HCI in dioxane (462 mL, 1.85 mol, 4.0 M). The mixture was stirred at ambient temp overnight. Concentration of the reaction mixture under reduced pressure afforded the title compound as a white solid (43 g, 98%). 400 MHz 1H NMR (DMSO) 5 3.85-3.82 (m, 2H), 3.27-3.21 (m, 2H), 3.13-3.05 (m, 1H), 1.88-1.84 (m, 2H), 1.48-1.38 (m, 2H). MS: (M+H m/z = 117.2). WO 2008/139293 PCT/IB2008/001125 -91- (b) 5-amino-1-(tetrahvdro-2H-pyran-4-vl)-1H-pyrazole-4-carbonitrile 10 15 20 To a mixture of 1-(tetrahydro-2H-pyran-4-yl)hydrazine di-hydrogen chloride (18g, 96 mmol) in 200 mL of EtOH was added Et3N (30g, 40 mL, 288 mmol) at 0°C (ice bath). The resulting mixture was stirred for 1 h, then a solution of 2-(ethoxymethylene)malononitrile (12 g, 96 mmol) in 100 mL of EtOH was added slowly to keep the reaction temp below 5 °C. This mixture was stirred at ambient temp overnight and then heated to reflux for 2 hr. After removal of the solvent under vacuum, the residue was washed with 300 mL of water. The solid was collected, washed with additional 200 mL of water, 200 mL of 1:1 of hexane and ether, dried to give 17 g of yellow solid. 400 MHz 1H NMR (CD3OD) 5 7.71 (s, 1H), 4.29-4.21 (m, 1H), 4.02 (dd, J = 11.6, 4.6 Hz, 2H), 3.28 (t, J = 1.7 Hz, 2H), 2.12-2.02 (m, 2H), 1.80-1.76 (m, 2H). MS: (M+H m/z = 193.1). (c) 5-amino-1 -(tetrahvdro-2H-pyran-4-vl)-1 H-pvrazole-4-carboxamide A stirred solution of 5-amino-1-(tetrahydro-2H-pyran-4-yl)-1H-pyrazole-4-carbonitrile (-228 mmol) in ethanol (300 mL) was treated with 35% aqueous H202 (100 mL) followed by aqueous ammonia (300 mL). The reaction mixture was stirred for 48 h at ambient temp and then quenched with aq saturated sodium thiosulfate (800 mL) and concentrated under reduced pressure to remove most of the ethanol. The resulting solid was removed by filtration and washed with water (2 x 200 mL) and ether (2 x 150 mL). The solid was dried in vacuo to constant weight (31 g, 65% yield for 2 steps). 400 MHz 1H NMR WO 2008/139293 PCT/IB2008/001125 -92- 15 20 (CD3OD) 5 7.67 (s, 1H), 4.27-4.21 (m, 1H), 4.03 (dd, J = 11.6, 4.6 Hz, 2H), 3.28 (t, J = 1.7 Hz, 2H), 2.14-2.04 (m, 2H), 1.81-1.78 (m, 2H). MS: (M+H m/z = 382.2). (d) 6-r(3.4-trans)-1-benzvl-4-methvlpyrrolidin-3-vl1-1-(tetrahvdro-2H-Pvran-4-vh-1.5-dihvdro-4H-pvrazolcf3.4-dlpyrimidin-4-one To a mixture of 5-amino-1-(tetrahydro-2H-pyran-4-yl-1H-pyrazole-4-carboxamide (6.0 g, 28.54 mmoi) and (3,4-trans)-methyl 1-benzyl-4-methylpyrrolidine-3-carboxylate (13.3 g, 57.08 mmol) was added molecular sieves (pellets). To the stirred mixture was added a 1.0 M solution of f-BuOK in THF (57.1 ml, 57.08 mmol) and the resulting mixture was heated at reflux under an atmosphere of nitrogen with vigorous stirring overnight. Analysis of the reaction mixture by LC/MS indicated consumption of the starting material. The reaction mixture was cooled to ambient temp and solids were removed by filtration. The solids were washed with EtOAc (2 x) and the combined filtrates were concentrated under reduced pressure. The remainder was partitioned between CH2CI2 and H20 and the aqueous and organic layers were separated. The aqueous phase was extracted with CH2CI2 (1 x) and the combined organic extracts were dried over Na2SC>4, filtered and concentrated under reduced pressure. The remaining residue was purified by chromatography (silica gel, 1 % Et3N in EtOAc) to afford the title compound (7.8 g, 70% yield) as an off-white solid. 400 MHz 1H NMR (CDCI3) § 8.02 (s, 1H), 7.39-7.25 (m, 6H), 4.83-4.75 (m, 1H), 4.14-4.09 (m, 2H), 3.82 (m, 1H), 3.62-3.54 (m, 3H), 3.39-3.37 (m, 1H), 3.00 (m,1H), 2.83 (m,1H), 2.66-2.27 (m, 4H), 2.10-1.83 (m, 3H), 1.20 (d, J = 6.6 Hz, 3H). MS: (M+H m/z = 394.2). WO 2008/139293 PCT/IB2008/001125 -93- EXAMPLE 61 6-f(,3S.4S)-1-benzyl-4-methvlpyrrolidin-3-vn-1-(tetrahydro-2H-pvran-4-vl)-1.5-dihvdro-4H-pyrazolor3.4-dlpyrimidin-4-one 5 The racemate of Example 60 was separated on Chiralcel OD-H chiral HPLC column, Mobile Phase 70/30 Heptane/EtOH, TR = 11.465, to provide the enantiomer. 400 MHz 1H NMR (CDCI3) 5 8.02 (s, 1H), 7.39-7.25 (m, 6H), 4.82-4.76 (m, 1H), 4.14-4.09 (m, 2H), 3.82-3.79 (m, 1H), 3.62-3.54 (m, 3H), 3.37 (d, J = 8.7 Hz, 1H), 3.00 (d, J = 9.9 Hz , 1H), 2.79 (dd, J = 6.3, 2.5 10 Hz , 1H), 2.52-2.48 (m, 1H), 2.42-2.30 (m, 3H), 1.94-1.82 (m, 3H), 1.20 (d, J = 6.6 Hz, 3H). MS: (M+H m/z = 394.2). EXAMPLE 62 (a) (3.4-trans)-ethvl-1-benzvl-4-(2.2.2-trifluoroethyl)pyrrolidine-3-carboxylate VJ 15 Following the procedure for the preparation of (3,4-trans)-methyl-1- benzyl-4-methylpyrrolidine-3-carboxylate but substituting <E)-methyl-5,5,5-trifluoropent-2-enoate provided the title compound. 400 MHz 1H NMR (CDCI3) 5 7.32-7.21 (m, 5H), 4.18-4.10 (m, 2H), 3.67-3.55 (m, 2H), 2.90-2.63 (m, 4H), 2.43-2.34 (m, 2H), 2.24-2.15 (m, 1H), 1.27-1.22 (m, 3H), 0.88-0.85 20 (m, 1H). (b) 6-r(3,4-trans)-1-benzvl-4-(2.2.2-trifluoroethyl)pvrrolidin-3-yl]-1-cyclopentvl-1.5-dihvdro-4H-pvrazolor3,4-dlpyrimidin-4-one WO 2008/139293 PCT/IB2008/001125 -94- [Rac] Following the procedure for the preparation of 6-[(3,4-trans)-1-benzyl-4-methylpyrrolidin-3-yl]-1-(2-methoxyphenyl)-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one but substituting 5-amino-1 -cyclopentyl-1 H-pyrazole-4-5 carboxamide and (3,4-trans)-ethyl-1-benzyl-4-(2,2,2-trifluoroethyl) pyrrolidine-3-carboxylate provided the title compound. 400 MHz 1H NMR (CDCI3) 5 8.02 (s, 1H), 7.36-7.26 (m, 5H), 5.12-5.08 (m, 1H), 3.85-3.81 (m, 1H), 3.60-3.57 (m, 1H), 3.42 (t, J = 8.3 Hz, 1H), 3,02-2.96 (m, 2H), 2.64 (m, 1H), 2.53-2.44 (m, 2H), 2.34-1.89 (m, 6H), 1.81-1.62 (m, 4H). 10 EXAMPLE 63 (a) 1-(4.4-difluorocyclohexv0hvdrazine To a solution of 4,4-difiuorocyclohexanol (0.9g) in toluene was added triphenyl phosphine (2.6g) and di-f-butyldiazacarboxalate (1.82g) and the 15 reaction mixture stirred for 18 h. The reaction mixture was concentrated and methanol was added (13mL). To the methanol solution HCI in dioxane (4M, 13 mL) was added. The reaction mixture was stirred for 3 h and concentrated. The reaction mixture was partitioned between water and ethyl acetate. The layers were separated and the aqueous layer was extracted 3x with ethyl 20 acetate. The organic layer was dried with magnesium sulfate, filtered and concentrated. The title compound was used without purification in the preparation of 5-amino-1-(4,4-difluorocyclohexyl)-1 H-pyrazole-4-carbonitrile. 400 MHz 1H NMR (CD3OD) 5 2.15-2.07 (m, 4H), 2.00-1.81 (m, 2H), 1.68-1.58 (m,2H). (M+H m/z = 279.0). WO 2008/139293 PCT/IB2008/001125 -95- (b) 5-amino-1-(4,4-difluorocyclohexvl)-1H-pvrazole-4-carbonitrile NC. Following the procedure for the preparation of 5-amino-1-(2-methoxyphenyl)-1 H-pyrazoie-4-carbonitrile but substituting 1 -(4,4-5 difluorocyclohexyl)hydrazine provided the title compound. 400 MHz 1H NMR (CDCb) 5 7.5 (m, 1H), 3.90 (m. 1H), 2.40-1.00 (m, 8H). (c) 5-amino-1 -(4.4-difluorocvclohexvn-1 H-pvrazole-4-carboxamide 10 methoxyphenyl)-1H-pyrazoie-4-carboxamide but substituting 5-amino-1-(4,4-difluorocyclohexyl)-1H-pyrazole-4-carbonitrile provided the title compound. 400 MHz 1H NMR (CD3OD) 5 8.01 (s, 1H), .4.19 (m, 1H), 2.22-1.90 (m, 8H). (d) 6-f(3.4-trans)-1-benzvl-4-methvlpyrrolidin-3-vn-1-(4.4-difluorocvclohexvl)-1.5-dihvdro-4H-Pvrazolor3.4-dlPvrimidin-4-one Following the procedure for the preparation of 6-[(3,4-trans)-1 -benzyl-4-methylpyrrolidin-3-yl]-1-(2-methoxyphenyl)-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one but substituting 5-amino-1-(4,4-difluorocyclohexyl)-1 H-pyrazole-4-carboxamide provided the title compound. 400 MHz 1H NMR Following the procedure for the preparation of 5-amino-1-(2- 15 WO 2008/139293 PCT/IB2008/001125 -96- 10 15 (CDCIs) 6 8.00 (s, 1H). 7.40-7.25 (m, 5H), 4.73-4.67 (m, 1 H), 3.85-3.82 (m, 1H), 3.64-3.61 (m, 1H), 3.38 (t, J = 8.7 Hz, 1H), 3.00 (m, 1H), 2.83 (m,1H), 2.56-2.52 (m, 1H), 2.43-2.25 (m, 5H), 2.04-1.90 (m, 5H), 1.20 (d, J = 7.1 Hz, 3H). MS: (M+H m/z = 428.1). EXAMPLE 64 (a) 5-amino-1-f2.2.2-trifluoroethvl)-1H-pvrazole-4-carbonitrile Following the procedure for the preparation of 5-amino-1-(2-methoxyphenyl)-1 H-pyrazole-4-carbonitrile but substituting 1-(2,2,2-trifluoroethyl)hydrazine provided the title compound. 400 MHz 1H NMR (CDCI3) 5 7.60 (s, 1H), 4.59 (m, 2H), 4.40 (brs, 2H). (b) 5-amino-1-(2.2.2-trifluoroethvl)-1 H-pvrazole-4-carboxamide Following the procedure for the preparation of 5-amino-1-(2-methoxyphenyl)-1H-pyrazole-4-carboxamide but substituting 5-amino-1-(2,2,2-trifluoroethyl)-1 H-pyrazole-4-carbonitrile provided the title compound. 400 MHz 1H NMR (DMSO) 5 7.75 (s, 1H), 7.30 (brs, 1H), 6.78 (brs, 1H) 6.58 (m, 2H), 4.88 (m, 2H). (c) 6-f(3.4-trans)-1-benzvl-4-methvlPvrrolidin-3-vn-1-f2.2.2-trifluoroethvl.)-1.5-dihvdro-4H-pvrazolor3.4-dlpyrimidin-4-one ] Following the procedure for the preparation of 6-[(3,4-trans)-1-benzyl-4-methylpyrrolidin-3-yl]-1-(2-methoxyphenyl)-1,5-dihydro-4H-pyrazolo[3,4- WO 2008/139293 PCT/IB2008/001125 -97- d]pyrimidin-4-one but substituting 5-amino-1-(2,2,2-trifluoroethyl)-1H-pyrazoie-4-carboxamide provided the title compound. 400 MHz 1H NMR (CDCI3) 5 8.15 (s,1H), 7.59-7.57 (m, 2H), 7.44-7.41 (m, 3H), 4.96^4.88 (m, 2H), 4.22-4.11 (m,2H), 3.57-3.37 (m, 4H), 2.85 (m, 2H), 1.26-1.23 (m, 3H). MS: (M+H m/z = 392.1). EXAMPLE 65 1-isopropvl-6-fC3S.4S)-4-methvl-1-f1.5-naphthvridin-4-vlmethvnpvrrolidin-3-vl]-1.5-dihvdro-4H-pvrazolor3.4-dlpyrimidin-4-one 10 Following the procedure for the preparation of 1-cyclopentyl-6-[(3,4- trans)-4-methyl-1-(pyridin-3-ylmethyl)pyrrolidin-3-yi]-1,5-dihydro-4H- pyrazolo[3,4-d]pyrimidin-4-one but substituting 1-isopropyl-6-[(3S,4S)-4- methylpyrrolidin-3-yl]-1 H-pyrazolo[3,4-d]pyrimidin-4(5H)-one and 1,5- naphthyridine-4-carbaldehyde provided the title compound. 400 MHz 1H NMR 15 (CDCI3) 5 9.09-9.08 (m, 1H), 8.95-8.87 (m, 1H), 8.44-8.39 (m, 1H), 8.00 (s, 1H), 7.73-7.70 (m, 1H), 7.67-7.64 (m, 1 H), 5.00-4.96 (m, 1H), 4.56-4.53 (m, 1H), 4.33-4.29 (m, 1H), 3.34 (t, J = 8.3 Hz , 1H), 3.19 (d, J = 9.5 Hz , 1H), 2.91-2.82 (m, 2H), 2.47-2.43 (m, 1H), 2.12 (t, J = 8.3 Hz, 1H), 1.48 (dd, J = 9.9, 6.6 Hz, 6H), 1.18 (d, J = 7.1 Hz, 3H). MS: (M+H m/z = 404.2). 20 EXAMPLE 66 1-isopropvl-6-r(3S.4SM-methvl-1-M.8-naDhthyridin-4-ylmethvl)Dvrrolidin-3-vll- 1.5-dihvdro-4H-pyrazolo[3.4-dlpvrimidin-4-one1 WO 2008/139293 PCT/IB2008/001125 -98- Following the procedure for the preparation of 1-cyclopentyl-6-[(3,4-trans)-4-methyl-1-(pyridin-3-ylmethyl)pyrrolidin-3-yl]-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one but substituting 1-isopropyl-6-[(3S,4S)-4-methylpyrrolidin-3-yl]-1 H-pyrazolo[3,4-d]pyrimidin-4(5H)-one and 1,8-5 naphthyridine-4-carbaldehyde provided the title compound. 400 MHz 1H NMR (CDCI3) 5 9.15 (dd, J = 4.1, 1.7 Hz, 1H), 9.07 (d, J = 4.6 Hz, 1H), 8.69 (dd, J = 8.3, 1.66 Hz, 1H), 7.99 (s, 1H), 7.69-7.65 (m, 1H), 7.46 (d, J = 4.6 Hz, 1H), 5.00-4.93 (m, 1H), 4.14 (s, 3H), 3.26 (t, J = 8.3 Hz , 1H), 3.03 (d, J = 9.5 Hz , 1H), 2.92-2.90 (m, 1H), 2.80-2.76 (m, 1H), 2.52-2.43 (m, 1H), 1.50-10 1.46 (m,6H), 1.20 (d, J = 6.6 Hz, 3H). MS: (M+H m/z = 404.1). EXAMPLE 67 1-isopropvl-6-f3S.4S)-4-methvl-1-fauinolin-4-vlmethvl)Pvrrolidin-3-vn-1,5-dihvdro-4H-Pvrazolor3.4-dlPvrimidin-4-one 15 Following the procedure for the preparation of 1-cyclopentyl-6-[<3,4- trans)-4-methyl-1-(pyridin-3-ylmethyl)pyrrolidin-3-yl]-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one but substituting 1-isopropyl-6-[(3S,4S)-4-methylpyrrolidin-3-yl]-1 H-pyrazolo[3,4-d]pyrimidin-4(5H)-one and quinoline-4-carbaldehyde provided the title compound. 400 MHz 1H NMR (CDCh) 5 20 10.63 (brs, 1H), 8.85 (d, J = 4.6 Hz, 1H), 8.26-8.24 (m, 1H), 8.13-8.11 (m, 1H), 7.99 (s, 1H), 7.77-7.69 (m, 2H), 7.39 (d, J = 4.6 Hz, 1H), 5.00-4.94 (m, 1H), 4.13 (d, J = 3.3 Hz, 2H), 3.32 (t, J = 8.3 Hz, 1H), 3.06 (d, J = 9.5 Hz, 1H), 2.89-2.87 (m, 1H), 2.74-2.70 (m, 1H), 2.46-2.42 (m, 1H), 2.04 (t, J =8.7 Hz, 1H), 1.48 (dd, J= 12.9, 7.1 Hz, 6H), 1.20 (d, J = 7.1 Hz, 3H). MS: (M+H 25 m/z 403.2). EXAMPLE 68 1-isopropvl-6-r(3S,4S)-4-methvl-1-(pvridof2.3-blpyrazin-8-vlmethvl)pyrrolidin- 3-vn-1.5-dihvdro-4H-pvrazolof3.4-dlpvrimidin-4-one WO 2008/139293 PCT/IB2008/001125 -99- Following the procedure for the preparation of 1-cyclopentyl-6-[(3,4-trans)~4-methyl-1-(pyridin-3-ylmethyl)pyrrolidin-3-yl]-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one but substituting 1-isopropyl-6-[(3S,4S)-4-5 methyipyrrolidin-3-yi]-1H-pyrazolo[3,4-d]pyrimidin-4(5H)-one and pyrido[2,3-b]pyrazine-8-carbaldehyde provided the title compound. 400 MHz 1H NMR (CDCIs) § 10.00 (brs, 1H), 9.16-9.10 (m, 3H), 8.00 (s, 1H), 7.78 (m, 1H), 5.00-4.97 (m, 1H), 4.63-4.58 (m, 1H), 4.34-4.18 (m, 1H), 3.30 (m,1H), 3.27 (m, 1H), 2.92-2.77 (m, 2H), 2.49-2.38 (m, 1H), 2.09-1.97 (m, 1H), 1.48 (dd, 10 J = 10.8, 6.6 Hz, 6H), 1.20 (d, J = 6.6 Hz, 3H). MS: (M+H m/z 405.2). EXAMPLE 69 1-isopropyl-6-{(3,4-trans)-1-[(6-methoxy-1,5-naphthyridin-4-yl)methyl]-4-methylpyrrolidin-3-yl}-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one [Rac] 15 Following the procedure for the preparation of 1-cyclopentyl-6-[(3,4- trans)-4-methyl-1-(pyridin-3-yimethyi)pyrrolidin-3-yl]-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one but substituting 1-isopropyl-6-[(3,4-trans)-4-methylpyrrolidin-3-yl]-1 H-pyrazolo[3,4-d]pyrimidin-4(5H)-one and 6-methoxy-1,5-naphthyridine-4-carbaldehyde provided the title compound. 400 MHz 1H 20 NMR (CDCIs) 5 8.78 (d, J = 4.6 Hz, 1H), 8.21 (d, J = 9.1 Hz, 1H), 8.01 (s, 1H), 7.63 (m,1H), 7.17 (d, J = 9.1 Hz, 1H), 5.01-4.95 (m, 1H), 4.4 (m, 1H), 4.07 (s, 3H), 3.48 (m,1H), 3.18 (m, 1H), 2.89-2.75 (m, 2H), 2.43 (m, 1H), 2.10-2.08 (m, 2H), 1.49 (dd, J = 11.6, 6.6 Hz, 6H), 1.21 (d, J = 6.6 Hz, 3H). MS: (M+H m/z 434.2). WO 2008/139293 PCT/IB2008/001125 -100-EXAMPLE 70 6-((3.4-trans)-H(8-f1uoroauinolin-2-vl)methvll-4-methvlpyrrolidin-3-yl)-1-isopropyl-1.5-dihydro-4H-pvrazolof3.4-dlpyrimidin-4-one trans)-4-methyl-1-(pyridin-3-ylmethyl)pyrrolidin-3-yl]-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one but substituting 1-isopropyl-6-[(3,4-trans)-4-methylpyrrolidin-3-yl]-1 H-pyrazolo[3,4-d]pyrimidin-4(5H)-one and 8-fluoroquinoline-2-carbaldehyde provided the title compound. 400 MHz 1H 10 NMR (CDCI3) 5 8.27-8.25 (m, 1H), 8.04 (s,1H), 7.86<m,1H), 7.66-7.60 (m, 1H), 7.50-7.38 (m, 2H), 5.04-4.97 (m, 1H), 4.30 (m,1H), 4.18 (m,1H), 3.47 (m, 2H), 3.28 (m,1H), 3.00 (m,1H), 2.59 (m,1H), 2.40 (m,1H), 1.50 (dd, J = 11.2, 6.6 Hz, 6H), 1.22 (d, J =7.1 Hz, 3H). MS: (M+H m/z 421.2). EXAMPLE 71 15 1-isopropvl-6-Uv)-1-r(6-methoxvauinolin-4-vnmethvn-4-methvlpvrrolidin-3-vl>-1.5-dihvdro-4H-pvrazolof3.4-dlpyrimidin-4-one trans)-4-methyl-1-(pyridin-3-ylmethyl)pyrrolidin-3-yl]-1,5-dihydro-4H-20 pyrazolo[3,4-d]pyrimidin-4-one but substituting 1-isopropyl-6-[(3,4-trans)-4-methylpyrrolidin-3-yl]-1 H-pyrazolo[3,4-d]pyrimidin-4(5H)-one and 6-methoxyquinoline-4-carbaldehyde provided the title compound. 400 MHz 1H NMR (CD3OD) 5 8.87 (d, J = 5.0 Hz, 1H), 8.08 (d, J = 9.5 Hz, 1H), 8.00-7.95 (m, 2H), 7.70 (d, J = 2.5 Hz, 1H), 7.61 (d, J= 2.9 Hz, 1H), 5.03-4.97 (m, 1H), 25 4.08 (s, 3H), 4.08-4.02 (m, 3H), 3.91-3.86 (m, 2H), 3.46-3.41 (m, 1H), 3.33 (s, WO 2008/139293 PCT/IB2008/001125 -101- 1H), 2.94 (m, 1H), 1.48 (d, J = 7.1 Hz, 3H), 1.42 (d, J= 6.6 Hz, 3H), 1.21 (d, J = 6.6 Hz, 3H). MS: (M+H m/z 433.2). EXAMPLE 72 (a) 5-amino-1 -cvclobutvl-1 H-pvrazole-4-carbonitrile cyclobutylhydrazine provided the title compound. 400 MHz 1H NMR (CDCI3) 5 7.50 (s, 1H), 4.48-4.40 (m, 1H), 4.23 (m, 2H), 2.70-2.58 (m, 2H), 2.48-2.35 10 (m, 2H), 1.97-1.79 (m, 2H). MS: (M+H m/z 163.1). (b) 5-amino-1 -cvclobutvl-1 H-pvrazole-4-carboxamide Following the procedure for the preparation of 5-amino-1-(2-methoxyphenyl)-1 H-pyrazole-4-carboxamide but substituting 5-amino-1-15 cyclobutyl-1H-pyrazole-4-carbonitrile provided the title compound. 400 MHz 1H NMR (CD3OD) 8 7.71 (s, 1H), 4.71-4.55 (m, 1H), 2.61-2.50 (m,2H), 2.46-2.31 (m, 2H), 1.89-1.83 (m, 2H). (c) 6-[(3,4-trans)-1-benzvl-4-methvlpyrrolidin-3-vl1-1-cvclobutyl-1,5-dihvdro-4H-pvrazolo[3.4-dlpyrimidin-4-one Following the procedure for the preparation of 6-[(3,4-trans)-1-benzyl-4-methylpyrrolidin-3-yl]-1-(2-methoxyphenyl)-1,5-dihydro-4H-pyrazolo[3,4- 5 Following the procedure for the preparation of 5-amino-1-(2-methoxyphenyl)-1 H-pyrazole-4-carbonitrile but substituting 1- 20 WO 2008/139293 PCT/IB2008/001125 -102- d]pyrimidin-4-one but substituting 5-amino-1-cyclobutyl-1H-pyrazole-4-carboxamide provided the title compound. 400 MHz 1H NMR (CDCI3) 6 8.03 (d, J = 3.32 Hz, 1H), 7.35-7.24 (m, 5H), 5.25-5.20 (m, 1H), 3.79-3.57 (m, 3H), 3.36-3.32 (m, 1H), 2.98 (d, J = 9.9 Hz, 1H), 2.80-2.60 <m, 3H), 2.53-5 2.38 (m, 3H), 1.92-1.79 (m, 3H), 1.25-1.12 (m, 3H). MS: (M+H m/z 464.2). yl)-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one (5.6g) was dissolved in 100 mL of methanol and added to a Parr bottle. Palladium hydroxide (3.76g) was added along with 3.56 mL of concentrated hydrochloric acid. The reaction mixture was placed on a hydrogenator under 40 psi of H2 for 18h. The 15 reaction mixture was filtered through Celite and concentrated to provide 4.47g of the title compound as the hydrogen chloride salt. 400 MHz 1H NMR (CD3OD) 5 8.03 (s, 1H), 4.49 (m,1H), 4.09-4.06 (m, 2H), 3.74-3.57 (m, 4H), 3.24 (m, 1H), 3.05 (m,1H), 2.89 (m, 1H), 2.77 (m, 1H), 2.30 (m, 2H), 1.90 (m, 2H), 1.22 (d, J = 6.6 Hz, 3H). MS: (M+H m/z = 304.2). 20 (b) 6-((3S.4S)-4-methvl-1-f(2-methvlpvrimidin-5-vl)methvllpyrrolidin-3-vl>-1-(tetrahvdro-2H-pyran-4-vl)-1.5-dihvdro-4H-pvrazolof3.4-dlpyrimidin-4-one EXAMPLE 73 (a) 6-f(3S.4S)-4-methvlpvrrolidin-3-vl1-1-(tetrahvdro-2H-pyran-4-vl)-1H- pvrazolo[3.4-dlpyrimidin-4(5H)-one 10 6-[(3S,4S)-1-benzyl-4-methylpyrrolidin-3-yl]-1-(tetrahydro-2H-pyran-4- WO 2008/139293 PCT/IB2008/001125 -103- To a solution of 6-[(3S,4S)-4-methylpyrrolidin-3-yl]-1-(tetrahydro-2H-pyran-4-yl)-1H-pyra2olo[3,4-d]pyrimidin-4(5H)-one hydrogen chloride (493 mg) in 1,2-dichloroethane (10mL) was added acetic acid (174 mg), 2-methylpyrimidine-5-carbaldehyde (236 mg) and sodium triacetoxy borohydride 5 (635 mg). The reaction mixture was heated at 50°C overnight. The reaction mixture was concentrated onto silica gel and purified by CombiFlash chromatography to provide the title compound (146 mg). 400 MHz 1H NMR (CDCIs) 5 8.63 (s, 2H), 8.01 (s, 1H), 4.82-4.76 (m, 1H), 4.12-4.08 (m, 2H), 3.68 (d, J = 5.0 Hz, 3H), 3.64-3.54 (m, 2H), 3.28 (t, J = 8.3 Hz, 1H), 3.04 (d, J 10 = 9.9 Hz, 1H), 2.89-2.86 (m, 1H), 2.71 (s, 3H), 2.66-2.62 (m, 1H), 2.49-2.27 (m, 3H), 1.97 (t, J = 7.9 Hz, 1H), 1.91-1.83 (m, 2H), 1.19 (d, J = 7.05 Hz, 3H). MS: (M+H m/z 410.2). EXAMPLE 74 6-IY3.4-trans)-4-ethvl-1-f(2-methylpvrimidin-5-vl)methvnDvrrolidin-3-vlM-15 (tetrahvdro-2H-pvran-4-vl)-1.5-dihvdro-4H-pyrazolof3.4-dlpyrimidin-4-one Following the procedure for the preparation of 6-{(3S,4S)-4-methyl-1-[(2-methylpyrimidin-5-yl)methyl]pyrrolidin-3-yl}-1-(tetrahydro-2H-pyran-4-yl)-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one but substituting 6-[(3,4-trans)-20 4-ethylpyrrolidin-3-yl]-1-(tetrahydro-2H-pyran-4-yl)-1 H-pyrazolo[3,4- d]pyrimidin-4(5H)-one hydrogen chloride provided the title compound. 400 MHz 1H NMR (CDCI3) 5 10.55 (brs, 1H), 8.64 (s, 2H), 8.01 (s, 1H), 4.81-4.73 (m, 1H), 4.11-4.09 (m, 2H), 3.68 (s, 2H), 3.61-3.53 (m, 2H), 3.28 (t, J = 8.7 Hz, 1H), 3.03 (d, J = 9.9 Hz, 1H), 2.94-2.92 (m, 1H), 2.71 (s, 3H), 2.57-2.53 25 (m, 1 H), 2.40-2.20 (m, 3H), 1.96 (t, J = 8.7 Hz, 1H), 1.89-1.84 (m, 2H), 1.64-1.57 (m, 1H). 1.52-1.44 (m, 1H), 0.91 (t, J = 7.1 Hz, 3H). MS: (M+H m/z 424.3). WO 2008/139293 PCT/IB2008/001125 -104- EXAMPLE 75 6-^3S.4S)-4-methvl-1-f(5-methvlPvrazin-2-vl)methvnpyrrolidin-3-vl>-1-ftetrahvdro-2H-pvran-4-vl)-1.5-dihvdro-4H-pvrazolor3.4-dlpyrimidin-4-one (Absl 5 Following the procedure for the preparation of 6-{(3S,4S)-4-methyl-1- [(2-methylpyrimidin-5-yl)methyl]pyrrolidin-3-yl}-1-(tetrahydro-2H-pyran-4-yl)-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one but substituting 5-methylpyrazine-2-carbaldehyde provided the title compound. 400 MHz 1H NMR (CDCb) 5 8.53 (s, 1H), 8.47 (s, 1H), 8.03 (s, 1H), 4.83-4.79 (m, 1H), 10 4.12-4.03 (m, 2H), 3.78-3.75 (m, 1H), 3.61-3.55 (m, 2H), 3.46-3.40 (m, 1H), 3.12-3.09 (m, 1H), 2.87 (m, 1H), 2.64 (m, 1H), 2.53 (m, 2H), 2.47-2.28 (m, 4H), 2.16 (m, 2H), 1.91-1.84 (m, 2H), 1.23-1.20 (m, 3H). MS: (M+H m/z 410.3). EXAMPLE 76 15 6-ff3S.4S)-1-[f6-methoxvpvridin-3-vnmethvn-4-methvlpyrrolidin-3-vl)-1-i,tetrahvdro-2H-pvran-4-vl)-1.5-dihvdro-4H-pvrazolof3.4-dlpvrimidin-4-one [Absl Following the procedure for the preparation of 6-{(3S,4S)-4-methyl-1-[(2-methylpyrimidin-5-yl)methyl]pyrrolidin-3-yl}-1-(tetrahydro-2H-pyran-4-yl)-20 1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one but substituting 6-methoxynicotinaldehyde provided the title compound. 400 MHz 1H NMR (CDCb) 5 8.03 (d, J = 2.1 Hz, 1H), 8.01 (s, 1H), 7.69 (dd, J = 8.7, 2.5 Hz, WO 2008/139293 PCT/IB2008/001125 -105- 15 1H), 7.77 (d, J= 8.7 Hz, 1H), 4.83-4.75 (m, 1H), 4.14-4.08 (m, 2H), 3.89 (s, 3H), 3.72-3.68 (m, 1H), 3.62-3.3.54 (m, 3H), 3.33 (t, J = 8.3 Hz, 1H), 2.99 (6, J = 9.9 Hz, 1H), 2.84-2.82 (m, 1H), 2.57-2.53 (m, 1H), 2.43-2.27 (m, 3H), 1.95-1.82 (m, 3H), 1.19 (d, J = 6.6 Hz, 3H). MS: (M+H m/z 425.3). 6-rf3S.4S)-4-methvl-1-fQuinolin-3-vlmethvl)Dvrrolidin-3-vll-1-(tetrahvdro-2H-pvran-4-vO-1.5-dihvdro-4H-Pvrazolor3.4-dlpyrimidin-4-one Following the procedure for the preparation of 6-{(3S,4S)-4-methyl-1-[(2-methylpyrimidin-5-yl)methyl]pyrrolidin-3-yl}-1-(tetrahydro-2H-pyran-4-yl)-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one but substituting quinoline-3-carbaldehyde provided the title compound. 400 MHz ^ NMR (CDCb) 5 8.86 (d, J = 2.1 Hz, 1H), 8.27 (d, J= 1.7 Hz, 1H), 8.08-8.06 (m, 1H), 8.03 (s,1H), 7.90-7.57 (m, 1H), 7.70-7.66 (m, 1H), 7.56-7.52 (m, 1H), 4.83-4.75 (m, 1 H), 4.11-4.07 (m, 2H), 4.02-3.99 (m, 1H), 3.85-3.82 (m, 1H), 3.60-3.53 (m, 2H), 3.33 (t, J = 8.3 Hz, 1H), 3.05 (d, J = 9.9 Hz, 1H), 2.87-2.85 (m, 1H), 2.68-2.60 (m,1H), 2.53-2.40 (m, 1H), 2.38-2.29 (m, 2H), 2.05 (m,1H), 1.90-1.84 (m,2H), 1.19 (d, J= 6.6 Hz, 3H). MS: (M+H m/z 445.1). EXAMPLE 78 6-((3S.4S)-4-methvl-1-r(2-methvlpvrimidin-4-vl)methvnpyrrolidin-3-vlH-(tetrahvdro-2H-pyran-4-vl)-1.5-dihvdro-4H-pvrazolof3.4-dlpyrimidin-4-one 5 EXAMPLE 77 WO 2008/139293 PCT/IB2008/001125 -106- Following the procedure for the preparation of 6-{(3S,4S)-4-methyl-1- [(2-methylpyrimidin-5-yl)methyl]pyrrolidin-3-yl}-1-(tetrahydro-2H-pyran-4-yl)- 1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one but substituting 2- methylpyrimidine-4-carbaldehyde provided the title compound. 400 MHz 1H 5 NMR (CDCb) 5 8.59 (d, J = 5.0 Hz, 1H), 8.03 (s, 1H), 7.20 (d, J = 5.0 Hz, 1H), 4.84-4.76 (m, 1H), 4.13-4.08 (m, 2H), 3.97-3.93 (m, 1H), 3.70-3.67 (m, 1H), 3.61-3.53 (m, 2H), 3.40 (t, J = 8.3 Hz, 1H), 3.14 (d, J = 9.5 Hz, 1H), 2.88 (m,1H), 2.72 (s, 3H), 2.69-2.63 (m, 1H), 2.47-2.27 (m, 3H), 2.10-2.08 (m, 1H), 1.91-1.83 (m, 2H), 1.21 (d, J = 7.1 Hz, 3H). MS: (M+H m/z 410.2). 10 EXAMPLE 79 6-^3S.4S)-4-methvl-1-f(6-methvlDvridin-3-vl)methvllDvrrolidin-3-vl)-1- (tetrahvdro-2H-Pvran-4-vn-1.5-dihvdro-4H-pyrazolof3.4-dlPvrimidin-4-one [Absl Following the procedure for the preparation of 6-{(3S,4S)-4-methyl-1-15 [(2-methylpyrimidin-5-yl)methyl]pyrrolidin-3-yl}-1 -(tetrahydro-2H-pyran-4-yl)-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one but substituting 6-methylnicotinaldehyde provided the title compound. 400 MHz 1H NMR (CD3OD) 5 8.38 (d, J = 2.1 Hz, 1H), 7.99 (s, 1H), 7.77 (d, J = 2.07 Hz, 1H), 7.75 (d, J = 2.07 Hz, 1H), 4.94-4.83 (m, 1H), 4.09-4.-05 (m, 2H), 3.78-3.57 20 (m, 4H), 3.31-3.28 (m, 1H), 3.11-3.06 (m, 1H), 3.01-2.91 (m, 2H), 2.72-2.65 6-r(3S.4S)-4-methyl-1-(f6-(trifluoromethyl)pyridin-3-vnmethvl>pvrrolidin-3-v 25 ftetrahvdro-2H-pvran-4-vl)-1.5-dihvdro-4H-PvrazoloI3.4-dlpyrimidin-4-one WO 2008/139293 PCT/IB2008/001125 -107- Following the procedure for the preparation of 6-{(3S,4S)-4-methyl-1-f(2-methylpyrimidin-5-yl)methyl]pyrrolidin-3-yl}-1-(tetrahydro-2H-pyran-4-yl)-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one but substituting 6-5 (trifiuoromethyl)nicotinaldehyde provided the title compound. 400 MHz 1H NMR (CDCIs) 5 8.64 (d, J = 1.7 Hz, 1H), 8.01 (s, 1H), 7.99 (d, J = 1.7 Hz, 1H), 7.70 (d, J = 8.3 Hz, 1H), 4.83-4.77 (m, 1H), 4.13-4.08 (m, 2H), 3.86-3.74 (m,2H), 3.61-3.53 (m, 2H), 3.30 (t, J = 8.7 Hz, 1H), 3.03 (d, J = 9.9 Hz, 1H), 2.92-2.89 (m, 1H), 2.72-2.67 (m, 1H), 2.50-2.47 (m, 1H), 2.38-2.30 (m, 10 2H), 2.07-2.03 (m, 1H), 1.91-1.82 (m, 2H), 1.20 (d, J = 7.1 Hz, 3H). MS: (M+H m/z 412.2). 6-((3S.4S)-4-methvl-1-f(1-methvl-1H-imidazof4.5-clpvridin-2-vnmethvnpvrrolidin-3-vl)-1-(tetrahvdro-2H-pyran-4-vl)-1,5-dihydro-4H-15 pvrazolof3,4-dlpyrimidin-4-one Following the procedure for the preparation of 6-{(3S,4S)-4-methyl-1-[(2-methylpyrimidin-5-yl)methyl]pyrrolidin-3-yl}-1-(tetrahydro-2H-pyran-4-yl)-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one but substituting 1-methyl-1 H-20 imidazo[4,5-c]pyridine-2-carbaldehyde provided the title compound. 400 MHz 1H NMR (CDCb) 5 10.83 (brs 1H), 9.01 (s, 1H), 8.41 (d, J = 15.8 Hz, 1H), 7.98 EXAMPLE 81 WO 2008/139293 PCT/IB2008/001125 -108- (s, 1H), 7.30 (d, J = 4.98 Hz, 1H), 4.79-4.75 (m, 1H), 4.13-4.06 (m, 3H), 4.00-3.96 (s, 4H), 3.60-3.52 (m, 2H), 3.30 (t, J = 8.7 Hz, 1H), 3.07 (d, J = 9.9 Hz, 1H), 2.92-2.89 (m, 1H), 2.82-2.77 (m, 1H), 2.48-2.45 (m, 1H), 2.36-2.27 (m, 2H), 2.19-2.17 (m, 1H), 1.89-1.79 (m, 2H), 1.23 (d, J = 6.6 Hz, 3H). MS: 5 (M+H m/z 449.2). EXAMPLE 82 6-lf3S.4S)-1-ff1.3-dimethvl-1H-pvrazol-5-vl)methvn-4-methvlDvrrolidin-3-vl>-1-(tetrahvdro-2H-pvran-4-vl)-1.5-dihvdro-4H-pyrazolof3,4-dlpyrimidin-4-one 10 [aSS) Following the procedure for the preparation of 6-{(3S,4S)-4-methyl-1-[(2-methylpyrimidin-5-yl)methyl]pyrrolidin-3-yl}-1-(tetrahydro-2H-pyran-4-yl)-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one but substituting 1,3-dimethyl-1H-pyrazole-5-carbaldehyde provided the title compound. 400 MHz 1H NMR (CDCI3) 5 7.98 (s, 1H), 5.94 (s, 1H), 4.81-4.75 (m, 1H), 4.57 (s,1H), 4.11-4.06 (m, 2H), 3.84 (s, 2H), 3.77 (s, 1H), 3.71-3.52 (m, 2H), 3.31 (t, J = 8.3 Hz, 1H), 3.01 (d, J = 9.9 Hz, 1H), 2.85-2.83 (m, 1H), 2.62-2.57 (m, 1H), 2.46-2.25 (m, 2H), 2.17-2.16 (m, 6H), 1.96-1.81 (m, 2H), 1.18 (d, J = 6.6 Hz, 3H). MS: (M+H m/z 412.2). EXAMPLE 83 (a) 1-cvclobutvl-6-r(3.4-trans)-4-methvlpyrrolidin-3-yn-1H-pyrazolof3.4- dlpyrimidin-4(5H)-one 0 20 25 WO 2008/139293 PCT/IB2008/001125 -109- Following the procedure for the preparation of 6-[(3S,4S)-4-methylpyrrolidin-3-yl]-1-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-d]pyrimidin-4(5H)-one but substituting 6-[(3,4-trans)-1-benzyl-4-methylpyrrolidin-3-yl]-1-cyclobutyl-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-5 one provided the title compound. 400 MHz 1H NMR (CD3OD) 8 8.03 (s,1H), 5.39 (m,1H), 3.79-3.38 (m, 4H), 3.08-2.71 (m, 4H), 2.43 (m, 2H), 1.92 (m, 2H), 1.22 (m,3H). MS: (M+H m/z 464.2). (b) 1-cvclobutvl-6-((3.4-trans)-4-methvl-1-f/2-methvlPvrimidin-5- vnmethvllPvrrolidin-3-vl}-1.5-dihvdro-4H-pyrazolor3.4-dlpyrimidin-4-one Following the procedure for the preparation of 6-{(3S,4S)-4-methyl-1-[(2-methylpyrimidin-5-yl)methyl]pyrrolidin-3-yl}-1-(tetrahydro-2H-pyran-4-yl)-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one but substituting 1-cyclobutyl-6-[(3,4-trans)-4-methylpyrrolidin-3-yl]-1H-pyrazolo[3,4-d]pyrimidin-4(5H)-one 15 provided the title compound. 400 MHz 1H NMR (CDCI3) 8 10.79 (brs, 1H), 8.63 (s, 2H), 8.04 (s,1H), 5.28-5.22 (m, 1H), 3.68 (s, 2H), 3.24 (t, J = 8.3 Hz, 1H), 3.04 (d, J = 9.9 Hz, 1H), 2.89-2.87 (m, 1H), 2.80-2.67 (m, 5H), 2.51-2.38 (m, 3H), 2.03-1.81 (m, 4H), 1.18 (d, J = 7.1 Hz, 3H). MS: (M+H m/z 480.2). 20 EXAMPLE 84 e-ffSS^Si-l-^.I.S-benzothiadiazol-S-vlmethvlM-methvlDvrrolidin-S-vn-l- ftetrahvdro-2H-pyran-4-vn-1.5-dihvdro-4H-Pvrazolof3.4-dlPvrimidin-4-one 10 WO 2008/139293 PCT/IB2008/001125 -110- [Abs) [(2-methylpyrimidin-5-yl)methyl]pyrrolidin-3-yl}-1-(tetrahydro-2H-pyran-4-yl)-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one but substituting 5 benzo[c][1,2,5]thiadiazole-5-carbaldehyde provided the title compound. 400 MHz 'H NMR (CDCI3) 5 10.97 (brs, 1H), 8.02-8.00 (m, 2H), 7.86 (s, 1H), 7.77 (d, J= 1.2 Hz, 1H), 4.81-4.75 (m, 1H), 4.12-4.06 (m, 2H), 3.96-3.92 (m, 1H), 3.81-3.78 (m, 1H), 3.60-3.52 (m, 2H), 3.38 (t, J = 8.3 Hz, 1H), 3.03 (d, J = 9.9 Hz, 1H), 2.88-2.85 (m, 1H), 2.68-2.63 (m, 1 H), 2.47-2.45 (m, 1H), 10 2.37-2.28 (m, 2H), 2.04 (t, J = 8.7 Hz, 1H), 1.90-1.80 (m, 2H), 1.21 (d, J = 7.1 Hz, 3H). MS: (M+H m/z 452.1), EXAMPLE 85 6-r(3S.4S)-4-methvl-1-fauinoxalin-2-vlmethvl)pvrrolidin-3-vll-1-(tetrahvdro-2H-Dvran-4-v0-1.5-dihvdro-4H-pvrazolof3.4-dlpvrimidin-4-one (mj Q3 ^ p xp 15 0 Following the procedure for the preparation of 6-{(3S,4S)-4-methyl-1- [(2-methylpyrimidin-5-yl)methyl]pyrrolidin-3-yl}-1-(tetrahydro-2H-pyran-4-yl)- 1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one but substituting quinoxaline-2- carbaldehyde provided the title compound. 400 MHz 1H NMR (CDCI3) 5 20 11.57 (brs, 1H), 8.89 (s,1H), 8.26 (dd, J = 8.3, 1.2 Hz, 1H), 8.08-8.05 (m, 2H), 7.78-7.70 (m, 2H), 4.82 (m, 1H), 4.32-4.28 (m, 1H), 4.13-4.08 (m, 2H), 4.00-3.96 (m, 1H), 3.63-3.55 (m, 2H), 3.44 (t, J = 8.3 Hz, 1H), 3.26 (d, J = WO 2008/139293 PCT/IB2008/001125 -111- 15 20 9.9 Hz, 1H), 2.93-2.91 (m, 1H), 2.70-2.69 (m, 1H), 2.39-2.31 (m, 2H), 2.25-2.23 (m,1H), 2.04 (s,1H), 1.92-1.84 (m, 2H), 1.23 (d, J = 7.1 Hz, 3H). MS: (M+H m/z 446.2). 6-ff3S.4SM-methvl-1-(auinolin-4-vlmethvnpvrrolidin-3-vl1-1-(tetrahydro-2H-Dvran-4-vn-1.5-dihvdro-4H-Dvrazolof3.4-dlpvrimidin-4-one Following the procedure for the preparation of 6-{(3S,4S)-4-methyl-1-[(2-methyJpyrimidin-5-yJ)methyJ]pyrroJidin-3-yJ}-1-(tetrahydro-2H-pyran-4-yl)-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one but substituting quinoline-4-carbaldehyde provided the title compound. 400 MHz 1H NMR (CDCI3) 5 10.68 (brs, 1H), 8.83 (d, J = 4.1 Hz, 1H), 8.25-8.22 (m, 1H), 8.12-8.08 (m, 1H), 7.98 (s,1H), 7.75-7.72 (m, 1H), 7.54 (d, J = 3.3 Hz, 1H), 7.36 (d, J = 3.3 Hz, 1H), 5.21 (m, 1H), 4.77 (m, 1H), 4.13-4.06 (m, 3H), 3.59-3.52 (m, 2H), 3.32 (d, J = 8.7 Hz, 1H), 3.03 (d, J = 9.9 Hz, 1H), 2.88-2.86 (m, 1H), 2.72-2.69 (m, 1H), 2.36-2.28 (m, 3H), 2.04-2.00 (m, 1H), 1.89-1.84 (m, 2H), 1.20 (d, J= 7.1 Hz, 3H). MS: (M+H m/z 445.1). 6-f(3S.4S)-4-methvl-1-(pvridin-2-vlmethyl)pyrrolidin-3-vn-1-(tetrahvdro-2H-pvran-4-vl)-1.5-dihvdro-4H-pvrazolo[3.4-d1pyrimidin-4-one EXAMPLE 86 EXAMPLE 87 WO 2008/139293 PCT/IB2008/001125 -112- To a solution of 6-[(3S,4S)-4-methylpyrrolidin-3-yl]-1-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-d]pyrimidin-4(5H)-one hydrogen chloride (3.98g) in dimethylformamide (60 mL) was added 2-pyridyl carbaidehyde (1.63g) followed by acetic acid (1.34 mL) and sodium triacetoxy borohydride (4.99g). 5 The reaction mixture was stirred for 20 minutes at ambient temperature and quenched with 24 mL of 1 N NaOH solution. The reaction mixture was adjusted to pH 9 with saturated sodium bicarbonate solution, extracted 3x with ethyl acetate and dried with magnesium sulfate, filtered and concentrated to give 3.6g of the title compound. The free base was dissolve in ethyl acetate 10 and 25 mL of HCI/ethylacetate was added and stirred. The white solid was filtered and dried to provide 5.10g of the title compound as a dihydrogen chloride salt. 400 MHz 1H NMR (CDCI3) 5 8.64-8.63 (m, 1H), 8.03 (s, 1H), 7.72-7.67 (m, 1H), 7.43 (d, J= 7.9 Hz, 1H), 7.21-7.18 <m, 1H), 4.85^.75 (m, 1H), 4.14-4.05 (m, 3H), 3.80-3.76 (m, 1H), 3.63-3.54 (m, 2H), 3.47-3.42 (m, 15 1H), 3.10 (m, 1H), 2.87 (m,1H), 2.66 (m, 1H), 2.46-2.28 (m, 3H), 2.14 (m, 1H), 1.93-1.84 (m, 2H), 1.20 (d, J = 7.1 Hz, 3H). MS: (M+H m/z 495.2). EXAMPLE 88 (a) 5-amino-3-methvl-1 -(tetrahvdro-2H-pvran-4-vh-1 H-pyrazole-4-carbonitrile Following the procedure for the preparation of 5-amino-1-(2-methoxyphenyl)-1H-pyrazole-4-carbonitrile but substituting 1-(tetrahydro-2H-pyran-4-yl)hydrazine and 2-(1-methoxyethylidene) malononitrile provided the title compound. 400 MHz 1H NMR (CD3OD) 5 4.20 (m, 1H), 4.05 (m, 2H), 25 3.50 (m, 2H), 2.18 (s, 3H), 2.09 (m, 2H), 1.77 (m, 2H). MS: (M+H m/z (b) 5-amir carboxamide WO 2008/139293 PCT/IB2008/001125 -113- Following the procedure for the preparation of 5-amino-1-(2-methoxyphenyl)-1H-pyrazole-4-carboxamide but substituting 5-amino-3-methyi-1-(tetrahydro-2H-pyran-4-yl)-1H-pyrazole-4-carbonitrile provided the 5 title compound. 400 MHz 1H NMR (CD3OD) 5 4.20 (m,1H), 4.05 (m, 2H), 3.50 (m, 2H), 2.18 (s, 3H), 2.09 (m, 2H), 1.77 (m, 2H). MS: (M+H m/z 225.0). (c) 6-r(3S.4S)-1-benzvl-4-methvlpyrrolidin-3-yl1-3-methvl-1-ftetrahvdro-2H-Dvran-4-vl)-1.5-dihvdro-4H-pvrazolof3.4-dlpyrimidin-4-one Following the procedure for the preparation of 6-[(3,4-trans)-1-benzyl- 4-methylpyrrolidin-3-yl]-1-(2-methoxyphenyl)-1,5-dihydro-4H-pyrazolo[3,4- d]pyrimidin-4-one but substituting 5-amino-3-methyl-1-(tetrahydro-2H-pyran-4- yl)-1 H-pyrazole-4-carboxamide and (3S,4S)-methyl-1-benzyl-4- 15 methylpyrrolidine-3-carboxylate provided the title compound. 400 MHz 1H NMR (CDCI3) 5 8.51-7.30 (m, 5H), 4.75-4.68 (m, 1H), 4.13-4.08 (m, 2H), 3.84- 3.73 (m,1H), 3.63-3.48 (m, 2H), 3.40 (m,1H), 3.10-2.78 (m, 2H), 2.55 (s, 3H), 2.50-2.24 (m, 3H), 1.87-1.80 (m, 2H), .61-1.41 (m, 3H), 1.19 (d, J = 6.6 Hz, 3H). MS: (M+H m/z = 408.1). 20 EXAMPLE 89 6-rf3S.4S)-1-(3-fluorobenzvl)-4-methvlpvrrolidin-3-vl1-1-(tetrahvdro-2H-pyran- 10 4-vl)-1,5-dihvdro-4H-pvrazolof3,4-dlpyrimidin-4-one WO 2008/139293 PCT/IB2008/001125 -114- Following the procedure for the preparation of 6-{(3S,4S)-4-methyl-1-[(2-methylpyrimidin-5-yl)methyl]pyrrolidin-3-yl}-1-(tetrahydro-2H-pyran-4-yl)-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one but substituting sodium 5 cyanoborohydride and 3-fluorobenzaldehyde provided the title compound. 400 MHz ]H NMR (CDCI3) 5 8.02 (s,1H), 7.35-7.30 <m, 1H), 7.21-7.20 (m, 1H), 7.05 (d, J = 9.5 Hz, 1H), 6.98-6.93 (m, 1H), 4.83-4.77 (m, 1H), 4.14-4.08 (m, 2H), 3.80-3.77 (m, 1H), 3.64-3.54 (m, 3H), 3.36 (t, J = 8.8 Hz, 1H), 3.01 (d, J = 9.9 Hz, 1H), 2.84-2.83 (m, 1H), 2.57 (m, 1H), 2.44-2.27 (m, 3H), 10 1.95-1.83 (m, 3H), 1.20 (d, J = 7.1 Hz, 3H). MS: (M+H m/z 412.4). 6-ff3S.4S)-1-(3.5-difluorobenzvP-4-methvlpvrrolidin-3-vn-1-(tetrahvdro-2H-Dvran-4-vl)-1.5-dihvdro-4H-pvrazolor3.4-dlpvrimidin-4-one [(2-methylpyrimidin-5-yl)methyl]pyrrolidin-3-yl}-1-(tetrahydro-2H-pyran-4-yl)-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one but substituting sodium cyanoborohydride and 3,5-difluorobenzaldehyde provided the title compound. 400 MHz 1H NMR (CDCIs) 5 8.03 (s, 1H), 6.93-6.90 (m, 2H), 6.73-6.69 (m, 20 1H), 4.81-4.80 (m, 1H), 4.14-4.10 (m, 2H), 3.79-3.67 (m, 2H), 3.62-3.55 (m, 2H), 3.30-3.26 (m, 1H), 3.10-3.07 (m, 1H), 2.95-2.92 (m, 1H), 2.83-2.79 (m, 1H), 2.26-2.53 (m, 1H), 2.38-2.32 (m, 2H), 2.18-2.14 (m, 1H), 1.91-1.85 (m, 2H), 1.18 (d, J = 7.1 Hz, 3H). MS: (M+H m/z 430.4). EXAMPLE 90 15 Following the procedure for the preparation of 6-{(3S,4S)-4-methyl-1- WO 2008/139293 PCT/IB2008/001125 -115- 10 15 EXAMPLE 91 6-^3S,4SM-methyl-1-r4-(trifluoromethyl)benzyllpyrrolidin-3-yl)-1-(tetrahydro-2H-pvran-4-vl)-1.5-dihydro-4H-pvrazolof3.4-dlpyrimidin-4-one (Absl [(2-methylpyrimidin-5-yl)methyl]pyrrolidin-3-yl}-1-(tetrahydro-2H-pyran-4-yl)-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one but substituting 4-(trifluoromethyl)benzaldehyde provided the title compound. 400 MHz 1H NMR (CDCI3) 5 8.03 (s, 1H), 7.62-7.59 (m, 2H), 7.51-7.49 (m, 2H), 4.83-4.76 (m, 1H), 4.14-4.08 (m, 2H), 3.87-3.84 (m, 1H), 3.70-3.67 (m, 1H), 3.61-3.54 (m, 2H), 3.34 (t, J = 8.3 Hz, 1H), 3.01 (d, J = 9.9 Hz, 1H), 2.88-2.86 (m, 1H), 2.67-2.62 (m, 1H), 2.48-2.46 (m, 1H), 2.38-2.30 (m, 2H), 2.05-2.00 (m, 1H), 1.81-1.82 (m, 2H), 1.19 (d, J = 7.1 Hz, 3H). MS: (M+H m/z 462.4). EXAMPLE 92 3-methyl-6-f(3S.4S)-4-methvl-1-(pvridin-3-vlmethvl)pvrrolidin-3-vl1-1-ftetrahvdro-2H-pvran-4-yl)-1.5-dihvdro-4H-pyrazolof3.4-dlpyrimidin-4-one [Abs] Following the procedure for the preparation of 6-{(3S,4S)-4-methyl-1-[(2-methylpyrimidin-5-yl)methyl]pyrrolidin-3-yl}-1-(tetrahydro-2H-pyran-4-yl)-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one but substituting 3-methyl-6-[(3S,4S)-4-methylpyrrolidin-3-yl]-1-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4- Following the procedure for the preparation of 6-{(3S,4S)-4-methyl-1- WO 2008/139293 PCT/IB2008/001125 -116- d]pyrimidin-4(5H)-one and nicotinaldehyde provided the title compound. 400 MHz 1H NMR (CDCb) 5 8.53 (m, 2H), 7.83 (d, J = 7.9 Hz, 1H), 7.36-7.33 (m, 1H), 4.74-4.68 (m, 1H), 4.12-4.07 (m, 2H), 3.79-3.76 (m, 1H), 3.68-3.65 (m, 1H), 3.59-3.52 (m, 2H), 3.47 (s, 1H), 3.34 (t, J = 8.7 Hz, 1H), 2.99 (d, J = 9.9 5 Hz, 1H), 2.82-2.80 (m, 1H), 2.61-2.57 (m, 1H), 2.54 (s, 3H), 2.42-2.28 (m, 2H), 1.95 (t, J= 8.7 Hz, 1H), 1.87-1.78 (m, 2H), 1.19 (d, J = 7.1 Hz, 3H). MS: (M+H m/z 409.2). (a) 3-methvl-6-f(3S.4S)-4-methvlpvrrolidin-3-vn-1-(tetrahvdro-2H-pyran-4-vl)-10 1H-pvrazolor3.4-d1pvrimidin-4(5H)-one Following the procedure for the preparation of 6-[(3S,4S)-4-methylpyrrolidin-3-yl]-1-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-d]pyrimidin-4(5H)-one but substituting 6-[(3S,4S)-1-benzyl-4-methylpyrrolidin-15 3-yl]-3-methyl-1-(tetrahydro-2H-pyran-4-yl)-1,5-dihydro-4H-pyrazolo[3,4- d]pyrimidin-4-one provided the title compound. 400 MHz 1H NMR (CDCb) 6 4.85-4.81 (m, 1H), 4.08-4.04 (m, 2H), 3.61-3.55 (m, 2H), 3.31-3.28 (m, 3H), 2.84-2.82 (m, 1H), 2.60-2.53 (m, 2H), 2.49 (s, 3H), 2.29-2.25 (m, 2H), 1.85-1.81 (m,2H), 1.12 (d, J = 6.2 Hz, 3H). MS: (M+H m/z = 249.1). 20 (b) 3-methvl-6-f(3S.4SM-methvl-1-f(2-methvlPvrimidin-5-vl)methvnpvrrolidin-3-vlM-ftetrahvdro-2H-pvran-4-vP-1,5-dihydro-4H-pyrazolor3.4-dlpvrimidin-4-one EXAMPLE 93 WO 2008/139293 PCT/IB2008/001125 -117- Following the procedure for the preparation of 6-{(3S,4S)-4-methyl-1-[(2-methyipyrimidin-5-yl)methyl]pyrrolidin-3-yl}-1-(tetrahydro-2H-pyran-4-yl)-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one but substituting 3-methyl-6-5 [(3S,4S)-4-methylpyrrolidin-3-yl]-1-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-d]pyrimidin-4(5H)-one provided the title compound. 400 MHz 1H NMR (CDCI3) S 8.63 (s, 2H), 4.74-4.68 (m, 1H), 4.11-4.08 (m, 2H), 3.74-3.63 (m, 2H), 3.60-3.52 (m, 2H), 3.28 (t, J= 8.3 Hz, 1H), 3.03 (d, J =9.5 Hz, 1H), 2.85-2.83 (m, 1H), 2.71 (s, 3H), 2.65-2.61 (m, 1H), 2.53 (s, 3H), 2.48-2.29 (m, 2H), 10 1.98 (t, J = 8.7 Hz, 1 H), 1.87-1.80 (m, 3H), 1.19 (d, J = 7.1 Hz, 3H). MS: (M+H m/z 424.2). EXAMPLE 94 6-(f3S.4S)-1-r(6-methoxvDvridin-3-vl)methvll-4-methvlPvrrolidin-3-yl)-3-methvl-1-(tetrahvdro-2H-pvran-4-vl)-1,5-dihvdro-4H-pvrazolof3.4-dlpyrimidin-4-one [toil Following the procedure for the preparation of 6-{(3S,4S)-4-methyl-1-[(2-methylpyrimidin-5-yl)methyl]pyrrolidin-3-yl}-1-(tetrahydro-2H-pyran-4-yl)-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one but substituting 3-methyl-6-[(3S,4S)-4-methylpyrrolidin-3-yl]-1-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4- WO 2008/139293 PCT/IB2008/001125 -118- d]pyrimidin-4(5H)-one and 6-methoxynicotinaldehyde provided the title compound. 400 MHz 1H NMR (CDCb) 5 8.03 (d, J = 2.1 Hz, 1H), 7.69 (dd, J = 8.7, 2.1 Hz, 1H), 6.78 (d, J= 9.1 Hz, 1H), 4.74-4.68 (m, 1H), 4.13-4.07 (m, 2H), 3.89 (s, 3H), 3.70-3.67 (m, 1H), 3.59-3.51 (m, 2H), 3.32 (t, J = 8.7 Hz, 5 1H), 2.97 (d, J = 9.9 Hz, 1H), 2.79-2.77 (m, 1H), 2.54 (s, 3H), 2.53-2.50 (m, 1H), 2.41-2.29 (m, 2H), 1.92-1.78 (m, 3H), 1.30-1.18 (m, 2H), 10.86 (t, J = 76.6 Hz, 3H). MS: (M+H m/z 439.2). 6-{(3S,4S)-4-methyl-1-[(6-methylpyridin-2-yl)methyl]pyrrolidin-3-yl}-1-10 (tetrahydro-2H-pyran-4-yl)-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one Following the procedure for the preparation of 6-{(3S,4S)-4-methyl-1-[(2-methylpyrimidin-5-yl)methyl]pyrrolidin-3-yl}-1-(tetrahydro-2H-pyran-4-yl)-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one but substituting sodium 15 cyanoborohydride and 6-methylpicolinaldehyde provided the title compound. 400 MHz 1H NMR (CDCI3) 5 8.02 (s, 1H), 7.57 (t, J = 7.7 Hz, 1H), 7.20 (d, J = 7.9 Hz, 1H), 7.03 (d, J = 7.5 Hz, 1H), 4.83-4.77 (m, 1H), 4.13-4.08 (m, 2H), 3.99-3.95 (m, 1H), 3.72-3.69 (m, 1H), 3.62-3.54 (m, 2H), 3.42 (t, J = 8.3 Hz, 1H), 3.06 (d, J = 9.9 Hz, 1H), 2.85-2.83 (m, 1H), 2.64-2.57 <m, 1H), 2.55 (s, 20 3H), 2.44-2.29 (m, 3H), 2.08-2.03 (m, 1H), 1.91-1.82 (m, 2H), 1.20 <t, J = 7.1 Hz, 3H). MS: (M+H m/z 409.1). 6-f(3S.4S)-1-(4-fluorobenzvl)-4-methvlPvrrolidin-3-vn-1-(tetrahvdro-2H-pvran-4-vl)-1.5-dihvdro-4H-pvrazolof3.4-dlpyrimidin-4-one EXAMPLE 95 EXAMPLE 96 WO 2008/139293 PCT/IB2008/001125 -119- Following the procedure for the preparation of 6-{(3S,4S)-4-methyl-1-[(2-methylpyrimidin-5-yl)methyl]pyrrolidin-3-yl}-1-(tetrahydro-2H-pyran-4-yl)-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one but substituting sodium 5 cyanoborohydride and 4-fluorobenzaldehyde provided the title compound. 400 MHz ^ NMR (CDCb) 5 8.02 (s, 1H), 7.36-7.33 (m, 2H), 7.05-7.01 (m, 2H), 4.82-4.77 (m, 1H), 4.14^.09 (m, 2H), 3.79-3.76 (m, 1H), 3.62-3.54 (m, 3H), 3.34 (t, J = 8.3 Hz, 1H), 2.99 (d, J = 9.9 Hz, 1H), 2.85-2.83 (m, 1H), 2.60-2.55 (m, 1H), 2.45-2.30 (m, 3H), 1.99-1.82 (m, 3H), 1.20 (d, J = 7.1 Hz, 3H). 10 MS: (M+H m/z 412.1). EXAMPLE 97 (a) 6-[(3.4-trans)-1-benzvl-4-ethvlPvrrolidin-3-vn-1-(tetrahvdro-2H-pvran-4-vl)-1.5-dihvdro-4H-pvrazolo[3.4-dlpyrimidin-4-one (b) 64(3S.4S)-1-benzvl-4-ethvlPvrrolidin-3-1.5-dihvdro-4H-Pvrazolor3.4-dlPvrimidin-4-one WO 2008/139293 PCT/IB2008/001125 -120- tV ; n The 6-[(3,4-trans)-1 -benzyl-4-ethylpyrrolidin-3-yl]-1 -{tetrahydro-2H-pyran-4-yl)-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one racemate was separated on Chiralcel OJ-H chiral HPLC column, Mobile Phase 80/20 5 C02/Me0H, TR = 3.27, to provide the enantiomer. Analytical: AD column, Mobile Phase 85/15 Heptane/EtOH, Tr = 12.896. 400 MHz 1H NMR (CDCI3) S 8.02 (s, 1H), 7.40-7.26 (m, 5H), 4.78 (m, 1H), 4.12-4.09 (m, 2H), 3.82-3.804 (m, 1H), 3.65-3.54 (m, 3H), 3.35 (t, J = 8.3 Hz, 1H), 2.98 (d, J = 9.9 Hz, 1H), 2.89-2.86 (m, 1H), 2.48-2.32 (m, 3H), 2.20 (m, 1H), 1.95-1.87 (m, 10 2H), 1.63-1.58 (m, 2H), 1.50-1.49 (m, 1H), 0.93 (t, J = 7.1 Hz, 3H). MS: (M+H m/z 408.1). EXAMPLE 98 6-rf3S.4S)-1-(2-fluorobenzvl)-4-methvlpyrrolidin-3-vn-1-(tetrahvdro-2H-Pvran-4-vH-1.5-dihvdro-4H-pvrazolof3.4-dlpvrimidin-4-one [Abs] 1 5 methylpyrimidin-5-yl)methyl]pyrrolidin-3-yl}-1-(tetrahydro-2H-pyran-4-yl)-1,5-25 dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one but substituting sodium cyanoborohydride and 2-fluorobenzaldehyde provided the title compound. 400 MHz 1H NMR (CDjOD) 5 7.98 (s,1H), 7.47-7.42 (m, 1H), 7.33-7.26 (m, 2H), 7.18-7.07 (m, 1H), 4.08-4.04 (m, 2H), 3.85 (s, 2H), 3.69-3.56 (m, 3H), WO 2008/139293 PCT/IB2008/001125 -121- 3.20-2.93 (m, 4H). 2.69-2.62 (m, 1H), 2.40-2.19 (m, 3H), 1.88-1.85 (m, 2H), 1.14 (d, J = 6.6 Hz, 3H). MS: (M+H m/z 412.1). EXAMPLE 99 6-((3S.4SM-methvl-1-r2-(trifluoromethvl)benzvHpvrrolidin-3-vl}-1-/tetrahvdro-5 2H-pvran-4-vlV1.5-dihvdro-4H-Dvrazolo[3.4-dlpyrimidin-4-one [Absl [(2-methylpyrimidin-5-yl)methyl]pyrrolidin-3-yl}-1-(tetrahydro-2H-pyran-4-yl)-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one but substituting sodium 10 cyanoborohydride and 2-(trifluoromethyl)benzaldehyde provided the title compound. 400 MHz 1H NMR (CDCI3) 5 10.58 (brs, 1H), 8.01 (s, 1H), 7.79-7.76 (m, 1H), 7.64-7.58 (m, 2H), 7.37-7.34 (m, 1H), 4.83-4.76 (m, 1H), 4.14-4.08 (m, 2H), 3.88 (m, 2H), 3.62-3.54 (m, 2H), 3.39 (t, J = 8.3 Hz, 1H), 3.02-2.97 (m, 1H), 2.85 (m,1H), 2.66 (m,1H), 2.44-2.27 (m, 3H), 2.06-2.03 15 (m, 1H), 1.91-1.83 (m, 2H), 1.21 (d, J = 7.1 Hz, 3H). MS: (M+H m/z 462.1). EXAMPLE 100 6-ff3S.4S)-1-(2.4-dif1uorobenzvlV4-methvlpvrrolidin-3-vn-1-(tetrahvdro-2H-Dvran-4-vl)-1.5-dihvdro-4H-Pvrazolof3.4-d1ovrimidin-4-one fAbs] 20 Following the procedure for the preparation of 6-{(3S,4S)-4-methyl-1- [(2-methylpyrimidin-5-yl)methyl]pyrrolidin-3-yl}-1 -<tetrahydro-2H-pyran-4-yl)-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one but substituting sodium cyanoborohydride and 2,4-difluorobenzaldehyde provided the title compound. WO 2008/139293 PCT/IB2008/001125 -122- 400 MHz 1H NMR (CDCb) 8 8.01 (s, 1H), 7.40-7.38 (m, 2H), 6.90-6.79 (m, 2H), 4.81-4.77 (m, 2H), 4.14-4.08 (m, 2H). 3.88 (m, 2H), 3.62-3.54 (m, 2H), 3.32 (t, J = 8.7 Hz, 1H), 3.02 (d, J = 9.5 Hz, 1H), 2.86-2.83 (m, 1H), 2.65 (m, 1H), 2.43-2.20 (m, 3H), 2.00-1.83 (m, 2H), 1.21 (d, J = 6.6 Hz, 3H). MS: (M+H m/z 430.2). EXAMPLE 101 6-IY3S.4S)-1-(4-methoxvbenzvlM-methylpvrrolidin-3-vn-1-(tetrahvdro-2H-Dvran-4-v0-1.5-dihvdro-4H-Pvrazolor3.4-dlpyrimidin-4-one 10 Following the procedure for the preparation of 6-{(3S,4S)-4-methyl-1- [(2-methylpyrimidin-5-yl)methyl]pyrrolidin-3-yl}-1-(tetrahydro-2H-pyran-4-yl)-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one but substituting sodium cyanoborohydride and 4-methoxybenzaldehyde provided the title compound. 400 MHz 1H NMR (CDCb) 8 8.01 (s, 1H), 7.29 (d, J = 8.3 Hz, 2H), 6.87 (d, J 15 = 8.7 Hz, 2H), 4.82-4.08 (m, 1H), 4.13-4.08 (m, 2H), 3.84-3.75 (m, 4H), 3.61-3.51 (m, 3H), 3.35 (t, J = 8.7 Hz, 1H), 2.97 (d, J = 9.9 Hz, 1H), 2.82-2.80 (m,1H), 2.53-2.49 (m, 1H), 2.42-2.30 (m, 3H), 1.95-1.82 (m, 3H), 1.18 (d, J = 7.1 Hz, 3H). EXAMPLE 102 20 (a) 5-amino-1-(tetrahvdro-2H-thiopvran-4-vn-1H-pyrazole-4-carbonitrile Following the procedure for the preparation of 5-amino-1-(2-methoxyphenyl)-1 H-pyrazole-4-carbonitrile but substituting 1-(tetrahydro-2H-thiopyran-4-yl)hydrazine provided the title compound. 400 MHz 1H NMR WO 2008/139293 PCT/IB2008/001125 -123- (CDCI3) 8 7.44 (s, 1H), 4.71 (s, 2H), 3.84-3.76 (m, 1H), 2.80-2.63 (m, 4H), 2.24-2.10 (m, 4H). MS: (M+H m/z 209.1). (b) 5-amino-1-(tetrahvdro-2H-thiopvran-4-vl)-1H-p.vrazole-4-carboxamide 5 Following the procedure for the preparation of 5-amino-1-(2- methoxyphenyl)-1H-pyrazole-4-carboxamide but substituting 5-amino-1-(tetrahydro-2H-thiopyran-4-yl)-1H-pyrazole-4-carbonitrile provided the title compound. 400 MHz 1H NMR (CDCI3) 8 7.67 (s,1H), 4.09-3.97 (m, 1H), 2.89-2.82 (m,2H), 2.72-2.68 (m, 2H), 2.15-2.10 (m, 4H). MS: (M+H m/z 10 227.1). (c) 6-f(3S.4S)-1-benzvl-4-methvlpyrrolidin-3-vn-1-(tetrahvdro-2H-thiopvran-4-vH-1.5-dihvdro-4H-pyrazolor3.4-d1ovrimidin-4-one (tosj1 H—— o Following the procedure for the preparation of 6-[(3,4-trans)-1-benzyl-15 4-methylpyrrolidin-3-yl]-1-(2-methoxyphenyl)-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one but substituting 5-amino-1-(tetrahydro-2H-thiopyran-4-yl)-1 H-pyrazole-4-carboxamide and (3S,4S)-methyl-1 -benzyl-4-methylpyrrolidine-3-carboxylate provided the title compound. 400 MHz 1H NMR (CDCI3) 8 8.02 (s, 1H), 7.41-7.26 (m, 5H), 4.60-4.53 (m, 1H), 3.86-3.83 (m, 1H), 3.65 (m, 20 1H), 3.41-3.37 (m, 1H), 3.02 (m, 1H), 2.94-2.75 (m, 4H), 2.60-2.31 (m, 3H), 2.25-2.16 (m, 2H), 1.99-1.94 (m, 1 H), 1.62 (m,2H), 1.20 (t, J = 7.1 Hz, 3H). MS: (M+H m/z 410.2). WO 2008/139293 PCT/IB2008/001125 -124- EXAMPLE 103 6-r(3S,4S)-1-f2-methoxvbenzyl)-4-methylpyrrolidin-3-yl]-1-(tetrahvdro-2H-pvran-4-vl)-1.5-dihvdro-4H-pyrazolor3.4-dlpyrimidin-4-one 5 Following the procedure for the preparation of 6-{(3S,4S)-4-methyl-1- [(2-methylpyrimidin-5-yl)methyl]pyrrolidin-3-yl}-1-(tetrahydro-2H-pyran-4-y()-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one but substituting sodium cyano borohydride and 2-methoxybenzaldehyde provided the title compound. 400 MHz 1H NMR (CDCIs) 8 8.00 (s, 1H), 7.29-7.22 (m, 2H), 6.92-6.88 (m, 2H), 10 4.83-4.75 (m, 1H), 4.14-4.08 (m, 2H), 3.93 (s, 3H), 3.76-3.69 (m, 2H), 3.62-3.54 (m,2H), 3.34 (t, J = 8.7 Hz, 1H), 3.01 (d, J = 9.5 Hz, 1H), 2.78 (m,1H), 2.56 (m,1H), 2.41-2.27 (m, 3H), 1.92-1.82 (m, 3H), 1.18 (d, J = 7.1 Hz, 3H). MS: (M+H m/z 424.1). EXAMPLE 104 15 6-f(3S.4S)-1-(3-methoxvbenzvl)-4-methvlpvrrolidin-3-vl1-1-(tetrahvdro-2H-pvran-4-vl)-1.5-dihvdro-4H-pvrazolor3.4-dlpvrimidin-4-one Following the procedure for the preparation of 6-{<3S,4S)-4-methyl-1-[(2-methylpyrimidin-5-yl)methyl]pyrrolidin-3-yl}-1-(tetrahydro-2H-pyran-4-yl)-20 1,5-dihydro~4H-pyrazolo[3,4-d]pyrimidin-4-one but substituting sodium cyanoborohydride and 3-methoxybenzaldehyde provided the title compound. 400 MHz 1H NMR (CDCI3) 8 8.02 (s, 1H), 7.27-7.21 (m, 1H), 6.96-6.92 (m, 2H), 6.81 (d, J = 7.1 Hz, 1H), 4.82-4.76 (m, 1H), 4.14-4.08 (m, 2H), 3.85 (s, WO 2008/139293 PCT/IB2008/001125 -125- 3H), 3.79 (s,1H), 3.62-3.54 (m, 3H), 3.39 (t, J = 8.7 Hz, 1H), 3.00 (d, J = 10.3 Hz, 1H), 2.81 (m, 1H), 2.53 (m, 1H), 2.42-2.28 (m, 3H), 1.94-1.82 (m, 3H), 1.20 (d, J= 7.1 Hz, 3H). MS: (M+H m/z 424.1). EXAMPLE 105 5 6-<Y3S.4SM-methvl-1-r3-(trifluoromethvl)benzynpyrrolidin-3-vl>-1-(tetrahvdro-2H-pyran-4-vlV1.5-dihvdro-4H-pvrazolof3.4-dlpyrimidin-4-one [Abs] [(2-methylpyrimidin-5-yl)methyl]pyrrolidin-3-yl}-1-(tetrahydro-2H-pyran-4-yl)-10 1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one but substituting sodium cyanoborohydride and 3-(trifIuoromethyl)benzaldehyde provided the title compound. 400 MHz 1H NMR (CDCI3) 5 8.02 (s,1H), 7.68-7.63 (m, 1H), 7.55-7.51 (m, 3H), 4.84-4.76 (m, 1H), 4.14-4.08 (m, 2H), 3.87-3.84 (m, 1H), 3.71-3.69 (m, 1H), 3.62-3.54 (m, 2H), 3.36 (t, J = 8.3 Hz, 1H), 3.01 (m, 1H), 15 2.86 (m,1H), 2.63-2.60 (m, 1H), 2.50-2.27 (m, 3H), 1.98-1.83 (m, 3H), 1.21 (d, J = 6.6 Hz, 3H). MS: (M+H m/z 462.1). EXAMPLE 106 6-ff3S.4S)-1-(26-difluorobenzvl)-4-methvlpvrrolidin-3-vn-1-(tetrahydro-2H-pvran-4-vl)-1.5-dihvdro-4H-pvrazolof3.4-dlpyrimidin-4-one (tos) Following the procedure for the preparation of 6-{(3S,4S)-4-methyl-1-[(2-methylpyrimidin-5-yl)methyl]pyrrolidin-3-yl}-1-(tetrahydro-2H-pyran-4-yl)-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one but substituting sodium cyanoborohydride and 2,6-difluorobenzaldehyde provided the title compound. WO 2008/139293 PCT/IB2008/001125 -126- 400 MHz 1H NMR (CDCI3) 5 8.00 (s,1H), 7.30-7.22 (m, 1H), 6.96-6.89 (m, 2H), 4.83-4.75 (m, 1H), 4.13-4.08 (m, 2H), 3.91 (s, 2H), 3.62-3.54 (m, 2H), 3.34 (t, J = 8.3 Hz, 1H), 3.06 (d, J = 9.5 Hz, 1H), 2.80 (m, 1H), 2.66 (m, 1H), 2.40-2.27 (m, 3H), 1.99 (m, 1H), 1.91-1.83 (m, 2H), 1.16 (d, J = 7.1 Hz, 5 3H). MS: (M+H m/z 430.1). (a) 6-f(3S.4S)-4-ethvlpvrrolidin-3-vl]-1-(tetrahvdro-2H-pvran-4-vl)-1H-pvrazolo[3.4-dlpyrimidin-4(5H)-one methylpyrrolidin-3-yl]-1-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-d]pyrimidin-4(5H)-one but substituting 6-[(3S,4S)-1-benzyl-4-ethylpyrrolidin-3-y|]-1-(tetrahydro-2H-pyran-4-yl)-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one provided the title compound. 400 MHz 1H NMR (CD3OD) 6 8.02 (s, 1H), 15 4.97 (m, 1H), 4.09-4.06 (m, 2H), 3.78-3.58 (m, 4H), 3.34-3.34 (m, 1H), 3.16-3.11 (m, 1H), 2.68 (d, J = 8.7 Hz, 1H), 2.63 (m, 1H), 2.32-2.27 (m, 2H), 1.90-1.87 (m, 2H), 1.69 (m,1H), 1.57 (m,1H), 0.97 (t, J = 7.5 Hz, 3H). MS: (M+H m/z 318.2). (b) 6-((3S.4S)-4-ethvl-1-f(5-methylpvrazin-2-vl)methvnpvrrolidin-3-vlV-1- 20 (tetrahvdro-2H-pvran-4-vl)-1.5-dihvdro-4H-pvrazolof3.4-d]pvrimidin-4-one To a solution of 6-[(3S,4S)-4-ethylpyrrolidin-3-yl]-1-(tetrahydro-2H- EXAMPLE 107 10 Following the procedure for the preparation of 6-[(3S,4S)-4- pyran-4-yl)-1H-pyrazolo[3,4-d]pyrimidin-4(5H)-one (4.4g) in WO 2008/139293 PCT/IB2008/001125 -127- dimethylformamide (62 mL) was added acetic acid (2.4 mL), 5-methylpyrazine-2-carbaldehyde (2g) and sodium triacetoxyborohydride (5.27g). The reaction mixture stirred for 2h at ambient temperature and was carefully quenched with saturated sodium bicarbonate solution, extracted 3x 5 with methylene chloride, dried with magnesium sulfate, filtered and concentrated. Purification via Biotage MPLC chromatography eluting with 1-4% methanol/methylene chloride/0.5 % saturated ammonium hydroxide provided the title compound (3.9g). 400 MHz 1H NMR (CDCI3) 5 8.52-8.48 (m, 1H), 8.38 (s, 1H), 8.02 (s, 1H), 4.81-4.75 (m, 1H), 4.11-4.01 (m, 3H), 10 3.79-3.75 (m, 1H) , 3.60-3.53 (m, 2H), 3.40-3.32 (m, 1H), 3.10-3.08 (m, 1H), 2.94 (m, 1H), 2.63-2.57 (m, 1H), 2.53 (d, J= 7.5 Hz, 1H), 2.37-2.18 (m, 4H), 1.90-1.83 (m, 2H), 1.67-1.60 (m, 1H), 1.54-1.47 (m, 1H), 0.95-0.92 (m, 3H). MS: (M+H m/z 424.2). 15 6-^3S,4S)-4-ethyl-1-f(6-methoxypyridin-3-yl)methyllpvrrolidin-3-vlM- ftetrahvdro-2H-pyran-4-vl)-1.5-dihvdro-4H-pyrazolor3.4-dlpyrimidin-4-one Following the procedure for the preparation of 6-{(3S,4S)-4-methyl-1-[(2-methylpyrimidin-5-yl)methyl]pyrrolidin-3-yl}-1-(tetrahydro-2H-pyran-4-yl)-20 1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one but substituting 6-[(3S,4S)-4-ethylpyrrolidin-3-yl]-1-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-d]pyrimidin-4(5H)-one hydrogen chloride, sodium cyanoborohydride and 6-methoxynicotinaldehyde provided the title compound. 400 MHz 1H NMR (CDCI3) 5 8.04 (d, J = 2.1 Hz, 1H), 8.02 (s, 1H), 7.73-7.71 (m, 1H), 6.78 (d, 25 J = 8.7 Hz, 1H), 4.80-4.75 (m, 1H), 4.12-4.10 (m, 2H), 3.92-3.90 (m, 3H), 3.72-3.70 (m, 1H), 3.61-3.54 (m, 3H), 3.32 (t, J = 8.3 Hz, 1H), 2.99 (m,1H), 2.91 (m, 1H), 2.41 (m, 1H), 2.40-2.28 (m, 2H), 2.21(m, 1H), 1.95 (m,1H), EXAMPLE 108 WO 2008/139293 PCT/IB2008/001125 -128- 1.91-1.83 (m, 2H), 1.65-1.45 (m, 2H), 0.92 (t, J = 7.5 Hz, 3H). MS: (M+H m/z 439.2). 6-[f3S.4S)-4-ethvl-1-(pvridin-2-vlmethvl)pyrrolidin-3-y|]-1-(tetrahvdro-2H-5 pvran-4-vn-1.5-dihvdro-4H-pvrazolof3.4-dlpvrimidin-4-one To a solution of 6-[(3S,4S)-4-ethylpyrrolidin-3-yl]-1-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-d]pyrimidin-4(5H)-one hydrogen chloride (51 mg) in acetonitrile (1 mL) was added potassium carbonate (88 mg) and 2-10 (bromomethyl)pyridine hydrogen bromide (40mg) and the reaction mixture was heated at reflux for 72 h in a sealed vial. The reaction mixture was concentrated onto silica and purified via CombiFlash flash chromatography to provide the title compound. 400 MHz 1H NMR (CDCI3) 5 11.50 (brs, 1H), 8.63 (dd, J= 1.7,0.83, Hz, 1H), 8.02 (s, 1H), 7.70-7.67 (m, 1H), 7.40 (d, J 15 = 7.9 Hz, 1H), 7.20-7.16 (m, 1H), 4.79-4.77 (m, 1H), 4.12-4.02 (m, 3H), 3.75-3.72 (m, 1H), 3.61-3.53 (m, 2H), 3.39 (t, J = 7.9 Hz, 1H), 3.07 (d, J = 9.9 Hz, 1H), 2.92-2.90 (m, 1H), 2.37-2.12 (m, 4H), 1.91-1.83 (m, 2H), 1.65-1.60 (m, 1H), 1.54-1.49 (m, 1H), 0.94 (t, J = 7.1 Hz, 3H). MS: (M+H m/z 409.1). 20 EXAMPLE 110 6-f(3S.4S)-4-ethyl-1-(Quinoxalin-2-vlcarbonvl)pvrrolidin-3-vl]-1-(tetrahvdro-2H- pyran-4-yl)-1.5-dihvdro-4H-pvrazolof3,4-dlpyrimidin-4-one EXAMPLE 109 WO 2008/139293 PCT/IB2008/001125 -129- To a solution of 6-[(3S,4S)-4-ethylpyrrolidin-3-yl]-1-{tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-d]pyrimidin-4(5H)-one hydrogen chloride (73 mg) in dicloromethane (2 mL) was added triethylamine (62 mg) and 2-quinoxaloylchloride (40 mg) at 0 °C. The reaction mixture was warmed to 5 ambient temperature and stirred for 18h. The reaction mixture was quenched with saturated sodium bicarbonate, extracted with methylene chloride, dried with magnesium sulfate, filtered and concentrated. Purification via CombiFlash flash chromatography eluting with 2-4% MeOH/methylene chloride provided the title compound. 400 MHz 1H NMR (CDCI3) 5 12.78-10 12.62 (m,1H), 9.41 (d, J = 7.5, Hz, 1H), 8.18-8.00 (m, 3H), 7.87-7.73 (m, 2H), 4.84-4.81 (m, 1H), 4.55-3.86 (m, 6H), 3.61-3.53 (m, 2H), 3.38-3.31 (m, 1H), 2.75 (m, 1H), 2.39-2.34 (m, 2H), 1.93-1.89 (m, 2H), 1.58-1.56 (m, 1H), 1.63-1.50 (m, 1H), 1.01-0.88 (m, 3H). MS: (M+H m/z 474.2). EXAMPLE 111 15 6-rf3S.4S)-4-methyi-1-('pvrimidin-2-ylmethv0pyrTOlid(n-3-vn-Wtetrahydro-2H-pvran-4-v0-1.5-dihvdro-4H-pyrazolo[3.4-dlpyrimidin-4-one [aEs] To a solution of 6-[(3S,4S)-4-methylpyrrolidin-3-yl]-1-(tetrahydro-2H-pyran-4-yi)-1H-pyrazolo[3,4-d]pyrimidin-4(5H)-one hydrogen chloride (7.75g) 20 (see preparation in step (a) of Example 73) in dimethylformamide (115 mL) was added iron triflate (900 mg), 2-(chloromethyl)pyrimidine hydrogen chloride (4.5g), and cesium carbonate (22.2g) and the reaction mixture was heated at 60 °C for 24h. The reaction mixture was concentrated onto silica gel and purified by flash chromatography eluting with 0-15% methanol/ethyl 25 acetate/1% saturated ammonium hydroxide to provide the title compound (6g). 400 MHz 1H NMR (CDCI3) § 12.30 (brs, 1H), 8.63 (d, J = 5.0, Hz, 2H), 8.03 (s, 1H), 7.20 (d, J = 5.0, Hz, 1H), 4.84-4.79 <m, 1H), 4.30-4.27 (m, 1H), WO 2008/139293 PCT/IB2008/001125 -130- 10 15 4.14-4.08 (m, 2H), 3.87-3.82 (m, 1H), 3.63-3.55 (m, 2H), 3.47 (t, J = 7.9 Hz, 1H), 3.29 (d, J = 9.9 Hz, 1H), 2.88-2.86 (m, 1H), 2.60-2.56 (m, 1H), 2.46-2.24 (m, 4H), 1.93-1.84 (m, 2H), 1.24 (t, J = 7.1 Hz, 3H). MS: (M+H m/z 396.2). 2-ffl3S.4S)-3-ethvl-4-r4-oxo-1-(tetrahvdro-2H-pvran-4-vlV45-dihvdro-1H-pvrazolor3.4-dlpvrimidin-6-vllpvrrolidin-1-vl)methvl)benzonitrile Following the procedure for the preparation of 6-{(3S,4S)-4-methyl-1-[(2-methylpyrimidin-5-yl)methyl]pyrrolidin-3-yl}-1-(tetrahydro-2H-pyran-4-yl)- I,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one but substituting 6-[(3S,4S)-4-ethylpyrrolidin-3-yl]-1-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-d]pyrimidin-4(5H)-one hydrogen chloride, sodium cyanoborohydride and 2-formylbenzonitrile provided the title compound. 400 MHz 1H NMR (CDCI3) 5 II.68 (brs, 1H), 8.01 (s, 1H), 7.68-7.58 (m, 3H), 7.40-7.36 (m, 1H), 4.91 (s, 1H), 4.82-4.76 (m, 1H), 4.12-4.10 (m, 2H), 3.94 (m, 1H), 3.61-6.55 (m, 2H), 3.37 (m,1H), 3.03-2.94 (m, 2H), 2.66 (m,1H), 2.41-2.23 (m, 3H), 2.09 (m, 1H), 1.91-1.84 (m, 2H), 1.66-1.49 (m, 2H), 0.93 (t, J = 7.5 Hz, 3H). MS: (M+H m/z 433.2). 3-f(f3S.4S)-3-ethvl-4-f4-oxo-1-(tetrahvdro-2H-pyran-4-vn-4.5-dihvdro-1H-pvrazolo[3,4-dlpyrimidin-6-ynpyrrolidin-1-yl)methvl)benzonitrile EXAMPLE 112 EXAMPLE 113 WO 2008/139293 PCT/IB2008/001125 -131- Following the procedure for the preparation of 6-{(3S,4S)-4-methyl-1-[(2-methylpyrimidin-5-yl)methyl]pyrrolidin-3-yl}-1-(tetrahydro-2H-pyran-4-yl)-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one but substituting 6-[(3S,4S)-4-ethyipyrrolidin-3-yl]-1-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-d]pyrimidin-5 4(5H)-one hydrogen chloride, sodium cyanoborohydride and 3-formylbenzonitrile provided the title compound. 400 MHz 1H NMR (CDCI3) 5 8.01 (s, 1H), 7.73-7.41 (m, 4H), 4.79 (m, 1H), 4.72 (s, 1H), 4.12-4.09 (m, 2H), 3.82-3.70 (m, 2H), 3.61-3.54 (m, 2H), 3.27 (t, J = 8.3 Hz, 1H), 3.02-2.97 (m, 2H), 2.71-2.70 (m, 1H), 2.37-2.30 (m, 2H), 2.20-2.14 (m, 1H), 10 1.90-1.87 (m, 2H), 1.61-1.60 (m, 1H), 1.51-1.49 (m, 1H), 0.91 (t, J = 7.5 Hz, 3H). MS: (M+H m/z 433.2). EXAMPLE 114 4-f^3S.4S)-3-ethvl-4-f4-oxo-1-(tetrahvdro-2H-pyran-4-vn-45-dihvdro-1H-Dvrazolor3.4-dlPvrimidin-6-vnpvrrolidin-1-vl)methvnbenzonitrile [(2-methylpyrimidin-5-yl)methyl]pyrrolidin-3-yl}-1-(tetrahydro-2H-pyran-4-yl)- I,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one but substituting 6-[(3S,4S)-4-ethylpyrrolidin-3-yl]-1-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-d]pyrimidin- 20 4(5H)-one hydrogen chloride, sodium cyanoborohydride and 4-formyibenzonitrile provided the title compound. 400 MHz 1H NMR (CDCI3) § II.82 (brs, 1 H), 8.01 (s, 1H), 7.75 (t, J = 7.9 Hz, 1H), 7.59-7.44 (m, 3H), 4.78-4.75 (m, 1H), 4.74 (s, 1H), 4.13-4.08 (m, 2H), 3.77 (m, 1H), 3.70 (m, 1H), 3.61-3.53 (m, 2H), 3.31-3.29 (m, 1H), 3.01-2.90 (m, 2H), 2.58 (m,1H), 25 2.37-2.31 (m, 2H), 2.25 (m,1H), 2.00 (m,1H), 1.91-1.83 (m, 2H), 1.64-1.63 (m, 1H), 1.61-1.59 (m, 1H), 0.93 (t, J = 7.5 Hz, 3H). MS: (M+H m/z 433.2). WO 2008/139293 PCT/IB2008/001125 -132- BIQLOGICAL PROTOCOLS The utility of the compounds of Formula (I), and the pharmaceutically acceptable salts thereof, in the treatment or prevention of diseases (such as are detailed herein) in mammals (e.g., humans) may be demonstrated by the 5 activity thereof in conventional assays known to one of ordinary skill in the art, including the assay described below. Such assays also provide a means whereby the activities of the compounds of Formula (I) can be compared with the activities of other known compounds. Phosphodiesterase 9 (PDE9) inhibitory activity 10 The PDE9 assay was performed using the Phosphodiesterase Scintillation Proximity (SPA) assay (GE Healthcare Life Sciences). The assay was carried out in 96 well clear bottom microtiter plates (Costar 3632, Corning Inc). The human recombinant PDE9 enzyme was generated in SF-9 cells, the cell pellets were sonicated in buffer (20 mM TRIS, 2mM benzamidine, 1mM 15 EDTA, 250mM sucrose, 100pM PMSF, pH 7.5 with HCI), centrifuged at 40,000 x g for 20 min at 4°C. The supernatants were stored at -80"C. [8- 3H]guanosine 3',5'-cyclic phosphate (TRK 392, GE Healthcare Life Sciences) was diluted in assay buffer (50 mM Tris-HCI, pH7.5, containing 1.3 mM MgCI2) such that the final well concentration was 50 nM. Test compounds 20 were dissolved in DMSO, diluted in Dl H20 and serially diluted in 20%DMSO/80%H20, for a final concentration of 2% DMSO. For the assay the PDE9 was diluted with assay buffer such that 20% or less of the substrate was hydrolyzed to 5'GMP. Each assay well contained 10 jjI of test compound or solvent, 40 pi of [3H]cGMP and 50 pi of enzyme, background was 25 determined by a high concentration of a PDE inhibitor. The assay was initiated with the addition of the enzyme and carried out at room temperature for 30 min. The assay was terminated with the addition of 10 pi of a PDE9 inhibitor that was sufficient to totally inhibit the enzyme activity, immediately followed by the addition of 50 pi per well of SPA beads. The plates were 30 sealed, vortexed, allowed to set for >300 min, then counted in a Wallac TriLux MicroBeta LSC. WO 2008/139293 PCT/IB2008/001125 -133- Example G5678A Example G5678A Example j G5678A No. (U):IC50 No. (U):IC50 No. (U):IC50 3 9.46 nM 43 36.4 nM 80 3.72 nM 4 624 nM 44 10.9 nM 81 2.92 nM 5 558 nM 45 38.7 nM 82 5.18 nM 6 57.1 nM 46 8.53 nM 83 24.5 nM 7 11.6 nM 47 5.53 nM 84 1.87 nM 8 11.2 nM 48 40.7 nM 85 0.903 9 2.84 nM 49 4.55 nM 86 1.44 nM 10 4.98 nM 50 125 nM 87 5.72 nM 11 18.9 nM 51 8.19 nM 88 17.9 nM 12 7.23 nM 52 26.0 nM 89 1.70 nM 13 6.61 nM 53 7.30 nM 90 1.41 nM 14 26.0 nM 54 14.7 nM 91 6.69 nM 17 16.2 nM 55 6.55 nM, 92 23.3 nM 18 8.26 nM 56 4.81 nM 93 24.3 nM 19 2.68 nM 57 122 nM 94 14.2 nM 20 7.06 nM 58 334 nM 95 3.92 nM 21 34.5 nM 59 7.37 nM 96 7.13 nM 22 43.6 nM 60 44.8 nM 97(a) 7.20 nM 23 1.32 nM 61 7.35 nM 97(b) 5.56 nM 24 119 nM 62 520 nM 98 7.93 nM 25 9.07 nM 63 123 nM 99 12.8 nM 26 11.4 nM 64 873 nM 100 22.7 nM 27 7.45 nM 65 17.1 nM 101 7.94 nM 28 6.86 nM 66 18.1 nM 102 19.7 nM 29 2.17 nM 67 9.74 nM 103 15.2 nM 31 23.2 nM 68 36.7 nM 104 3.98 nM 32 5.19 nM 69 30.7 nM 105 3.29 nM 33 6.29 nM 70 10.2 nM 106 8.06 nM 34 4.33 nM 71 16.1 nM 107 4.33 nM 35 53.8 nM 72 40.1 nM 108 3.11 nM 36 5.08 nM 73 6.01 nM 109 4.21 nM 37 3.23 nM 74 6.14 nM 110 2.59 nM 38 5.75 nM 75 5.46 nM 111 12.5 nM 39 58.1 nM 76 3.50 nM 112 1.37 nM 40 44.5 nM 77 1.24 nM 113 <1.00 41 63.8 nM 78 4.35 nM 114 2.31 nM 42 268 nM 79 3.72 nM | The following additional compounds were made in accordance with the methods set forth above: WO 2008/139293 PCT/IB2008/001125 -134- Ex. No Structure IUPACNAME G5678A (U):IC50 Exact Mass Molec. Weight Obs. m/z (M+1) Retention Time 115 ^V V b 1 -cyclopentyl-6-{(3.4-trans)-4-methyi-1 -[3-(1 H- pyrazol-1-yl)benzyl]pyrrolidin-3-yl}- 1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one 40.4 nM 443.243 557.579 444.28 2.71 116 [RacJ >-o b" 15 ry1 1-cyclopentyl-6-{(3,4-trans)-4-methyl-1-[(2-methylpyridin-4-yl)methyl]pyrrolidin-3-yl}- 1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one 34.0 nM 392.233 620.556 393.32 2.04 117 [R«c! Vvx s ^ 6C 6-[(3,4-trans)-1 -(2-chloro- 6-fiuorobenzyl)-4-methylpyrrolidin-3-yl]-1 -cyclopentyl-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one 126 nM 429.173 543.951 430.23 2.7 118 7ifK ' 1-cyclopentyl-6-[(3,4-trans)-1-(2,3- dimethy1benzyl}-4-methylpyrrolidin-3-yl]-1.5-dihydro-4H-pyrazolo(3,4-d]pyrimidin-4-one 74. nM 405.253 519.57 406.32 2.9 119 !a*ci ■hci Kw o V\, i 1-cyclopentyl-6-((3.4-trans)-1-[2-(difluoromethoxy)benzyl]-4-methylpyrrolidin-3-yl}- 1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one 32.8 nM 443.213 557.522 444.28 2.82 WO 2008/139293 PCT/IB2008/001125 -135- 120 LRacl bO K"' b k 1 -cyclopentyl-6-{(3,4-trans)-1-[(2-ethoxypyridin-3-yl)methyl]-4-methylpyrrolidin-3-yl}-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one 62.7 nM 422.243 650.582 423.3 2.66 121 !_RflC \_/v <fy" 1-cyclopentyl-6-[(3.4-trans}-4-methyl-1-(4,5,6,7-tetrahydropyrazolo[1,5- ajpyridin-3-ylmethyl)pyrrolidin-3-yl]- 1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidln-4-one 83.9 nM 421.259 535.573 422.33 2.43 122 /V vf>_b 1-cyclopenlyl-6-[(3.4-trans)-1 -(2,3-dihydro-1,4-benzodloxin-6-ytmethyl)-4-methylpyrrolid ln-3-yl]- 1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one 56.4 nM 435.227 549.552 436.28 2.68 123 V._/~ "SQ \~"' 1-cyclopentyl-6-{(3,4-trans)-1-(4-(1 H-imidazol-1-yl)benzyl]-4-methylpyrrolidin-3-yl}-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one 39.3 nM 443.243 557.579 444.29 2.11 124 y~cii . \-Y V- k o , \/ Y 1-cyclopentyl-6-[(3.4-tra ns)-1 -(2,5-dichlorobenzyl)-4-methylpyrrolidin-3-yl]-1.5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one 60.9 nM 445.144 560.406 446.19 2.92 WO 2008/139293 PCT/IB2008/001125 -136- 125 I dir. \ /V \/ 1-cyclopentyl-6-[(3,4-trans)-1-(4-methoxy-3-methylbenzyl)-4-methy1pyrrolidin-3-yl]-1,5-dihydro-4H-pyrazolo[3.4-d]pyrimidin-4-one 55.2 nM 421.248 535.569 422.31 2.89 126 \—./x < M \ s,"\ rv-1 vs V 1-cyclopentyl-6-[(3,4-trans)-1-(2,3-dihydrc-1-benzofuran-7-ylmethyl)-4-methy1pyrrolidin-3-yl]- 1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one 88.7 nM 419.232 533.553 420.31 2.75 127 y- 1-cyclopentyl-6-[(3,4-trans)-1-(2,3-dif!uorobenzyl)-4-methylpyrrolidin-3-yl]-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one 61.7 nM 413.203 527.496 414.28 2.73 128 )V\ >-Pb 1-cyclopentyl-6-[(3,4-trans)-1-(5-fluoro-2-methoxybenzyl)-4-m ethylpyrrolidin-3-yl]-1.5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one 70.9 nM 425.223 539.532 426.29 2.8 129 :3XJ '^tAs __ /~~ \ \r - 1-cyclopentyl-6-[(3,4-trans)-1-(2-fluoro-4-methoxybenzyl)-4-methy1pyrrolidin-3-yl]-1.5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one 71.8 nM 425.223 539.532 426.29 2.75 130 L52.c; 1 -cyclopentyl-6-[(3,4-trans)-1-(3-fluoro-4-methylbenzyl)-4-methylpyrrolidin-3-yl]-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one 60.0 nM 409.228 523.533 410.28 2.87 WO 2008/139293 PCT/IB2008/001125 -137- 131 w>, \ V\ Q ^ 1 -cyclopenty l-6-{(3.4-trans)-4-methyl-1-[(2-methyl-1,3-thiazol-5-yl)methyl]pyrrolidin-3-yi}- 1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one 41.9 nM 398.189 512.557 399.23 2.32 132 V /"i/ / \ 1-cyclopentyl-6-{(3,4-trans)-1-[(4-isopropyl-1,3-thiazol-2-yl)methyl]-4-methylpyrrolidin-3-yl}- 1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one 37.5 nM 426.22 540.611 427.28 2.83 133 > 1-cyclopentyl-6-{(3,4-trans)-1 -[(1,3-dimethyl-1 H-pyrazol-5-yl)methyl]-4-methylpyrrolidin-3-yl}- 1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one 33.7 nM 395.243 509.535 396.31 2.35 134 V-/s y-^X) 1 -cyclopentyl-6-[(3.4- trans)-1-(2,3-difluoro-4- methylbenzyl)-4-methylpyrrolidin-3-yl]-1,5-dihydro-4H-pyrazolc![3,4-d]pyrimidin-4-one 93.5 nM 427.218 541.523 428.29 2.89 135 V>r"b 1 -cyclopentyl-6-[(3.4-trans)-4-methyl-1 -{[6-(1 H-pyrazol-1-yl)pyridin-2-yl]methyl}pyrrolidin-3- yl]-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one 91.6 nM 444.239 672.592 445.3 2.59 136 ;RlC' >P\> \ / 1 -cyclopentyl-6-[(3.4-trans)-4-methyl-1-(4- methylbenzyl)pyrrolidin- 3-yl]-1,5-dihydro-4H-pyrazolo[3.4-d]pyrimidin-4-one 132 nM 391.237 505.543 392.31 2.81 WO 2008/139293 PCT/IB2008/001125 -138- 137 Hit 1-eyclopentyl-6-[(3,4-trans)-4-methyl-1-(2-naphthylmethyt)pyrrolidin -3-yI]-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one 54.5 nM 427.237 541.576 428.3 2.97 138 :rk. LA, vr b 1-cyclopentyl-6-{(3.4-trans)-1-[(2-methoxypyridin-3-yl)methyl]-4-methylpyrrolidin-3-yl}-1,5-dihyd ro-4H-pyrazolo[3,4-d]pyrimidin-4-one 47.7 nM 408.227 636.555 409.29 2.5 139 b"b \ ;■■ \ 1-cyclopentyl-6-[(3,4-trans)-1 -(2-ethoxybenzyl)-4-methylpyrrolidin-3-yl]-1.5-dihydro-4H-pyrazolo[3.4-d]pyrimidin~4-one 97.5 nM 421.248 535.569 422.33 2.88 140 [Hac_ \y 1-cyclopentyl-6-{(3,4-trans)-4-methyl-1-[4-(1 H- 1,2.4-triazol-1 -yl)benzyl]pyrrolidin-3-yl}- 1,5-dihydro-4H-pyrazolo[3,4-dJpyrimidin-4-one 26.8 nM 444.239 558.567 445.3 2.42 141 ■H»;. >Y> \ r'y A 1-cyclopentyl-6-[(3.4-trans)-1-(3-methoxy-4-methylbenzyl)-4-methyipyrrolidin-3-yl]-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one 53.1 nM 421.248 535.569 422.3 2.94 WO 2008/139293 PCT/IB2008/001125 -139- 142 1 -cyc!opentyl-6-[(3.4-trans)-4-methyl-1-(1-naphthylmethyljpyrroiidin -3-yl]-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one 47.5 nM 427.237 541.576 428.3 2.93 143 N: X \/ \ XX 1 -cyclopentyl-6-[(3,4-trans)-1-(3-fluoro-4- methoxybenzyl)-4-methy1pyrrolidin-3-yl]-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one 35.1 nM 425.223 539.532 426.29 2.73 144 jq»c \ XV " XX I 1 -cyclopentyl-6-[(3,4- trans>-1 -(2,5-dimethoxybenzyl)-4-methylpyrrolidin-3-yl]-1,5-dihydro-4H-pyrazolo[3,4-dJpyrimidin-4-one 99.6 nM 437.243 551.568 438.29 2.79 145 ifite hQ 1-cyclopentyl-6-{(3.4-trans)-4-methyl-1-[(5-melbylisoxazol-3-yl)methytJpyrrolidin-3-yl}- 1,5-dihydro-4H-pyrazo[o[3,4-d]pyrrmidin-4-one 77.4 nM 382.212 496.492 383.27 2.5 146 3±i. XV v vy r"\ - V ^ ex 1 -cyclopentyl-6-[(3.4-trans)-1-(2-fluoro-6-methoxybenzyl)-4-methylpyrrolidin-3-yl]-1,5-dihydro-4H-pyrazolo[3,4-dJpyrimidin-4-one 165 nM 425.223 539.532 426.29 2.76 147 [±m£, >-r? \__}—\ V ^ u 1 -cyclopentyl-6-[(3.4-trans)-1 -(2,4-difluorobenzyl)-4-methy1pyrro!idin-3-yl]-1,5-dihydro-4H-pyrazo!o[3,4-d]pyrimidin-4-one 79.8 nM 413.203 527.496 414.28 2.72 WO 2008/139293 PCT/IB2008/001125 -140- 148 Vv"V b b ] 1-cyclopentyl-6-[(3.4-trans)-1-(4-fluoro-3-methoxybenzyl)-4-methy1pyrrolidin-3-yl]-1,5-dihydro-4H-pyrazolof3,4-d]pyrimidin-4-one 36.0 nM 425.223 539.532 426.29 2.79 149 :r*j V/X ^'b b5 1-cyclopentyl-6-[(3,4-trans)-1-(2,3-dihydro-1,4-benzodioxin-5-ylmethyl}-4-methylpyrrolidin-3-yl]- 1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one 47.1 nM 435.227 549.552 436.28 2.73 150 t**g: 6-[(3,4-trans)-1 -(2-chbro- 4-fluorobenzyl)-4-methylpyrrolidi n-3-yl]-1 -cyclopentyl-1,5-dihydro-4H-pyrazolo[3,4-]pyrimidin-4-one 93.5 nM 429.173 543.951 430.23 2.82 151 [R*cJ )-bb b"° 1 -cyclopentyl-6-[(3,4- trans)-1-(2,4-dimethyfbenzyl)-4-methylpyrrolidin-3-yl]-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one 117 nM 405.253 519.57 406.32 2.92 152 IK": v' \ 1-cyclopentyl-6-[(3,4- trans)-1-(3,5-dimethoxybenzyl)-4-methytpyrrolidin-3-yl)-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one 42.2 nM 437.243 551.568 438,29 2.8 WO 2008/139293 PCT/IB2008/001125 -141- 153 _KJ., s /' \ V- o 1-cyclopentyl-6-[(3,4-trans)-1-(3-ethoxybenzyl)-4-methylpyrrolidin-3-yl}-1.5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one 54.4 nM 421.248 535.569 422.28 2.88 154 LR*eJ y~/v < \J \h" V"\ 9 ^ 6-[(3.4-trans)-1 -(4-chloro- 2-fluorobenzyl)-4-methylpyrrolidin-3-yl]-1 -cyclopentyl-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one 175 nM 429.173 543.951 430.23 2,88 155 i-/v V>v \ / * ' \ b ^ <v> i ,i| 3-{I(3,4-trans)-3-(1-cyclopenty)-4-0X0-4,5-dihydro-1 H-pyrazolo[3,4-d]pyrimidin-6-yl)-4-methylpyrrolidin-1-yl]methyl}benzonitrile 27.9 nM 402.217 516.526 403.25 2.61 156 '.Mac.1 \ J~'\ Q 1-cyclopentyl-6-[(3,4-trans)-1-(2l5-difluorobenzy])-4-m ethylpyrrolidin-3-yl]-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one 40.8 nM 413.203 527.496 414.27 2.69 157 ;RJCJ \^P* 2-{[(3,4-trans)-3-(1-cyclopentyl-4-0X0-4,5-dihydro-1 H-pyrazolo[3,4-d]pyrimidin-6-yl)-4-methylpyrrolidin-1-yl]methyl}benzonitnle 56.4 nM 402.217 516.526 403.26 2.6 WO 2008/139293 PCT/IB2008/001125 -142- 158 .Rac. M-vb b j a 6-[(3,4-trans)-1-(3-chloro- 4-fluorobenzyl)-4-methylpyrrolidin-3-yl]-1 -oyclopentyl-1,5-dlhydro-4H-pyrazolo[3,4-d]pyrimidin-4-one 50.9 nM 429.173 543.951 430.21 2.91 159 .iWj YCj I ; S 1-cyclopentyl-6-{(3,4-trans)-1-[4-(difluoromethoxy)benzyl]-4-methylpynolidin-3-yi}- 1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one 59,9 nM 443.213 557.522 444.26 2.88 160 IRic) b' ^ ! 1 -cyclopentyl-6-[(3.4-trans)-4-methyl-1-(3-methylbenzyl)pyrrolidin- 3-yl]-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one 43.4 nM 391.237 505.543 392.29 2,8 161 .Hid / VJ vAy b 1-cyclopentyl-6-[(3.4-trans)-1 -(3,4-difluorobenzyl)-4-methy1pyrrolidin-3-yl]-1.5-dihydro-4H-pyrazolo[3.4-d]pyrimidin-4-one 40.4 nM 413.203 527.496 414.26 2.8 162 i-ftac; v bv w 0 b/ 1 -cyclopentyl-6-[(3.4- trans)-1-(2,5-dimethy)benzyl)-4-methylpyrrolidin-3-yl]-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one 60.1 nM 405.253 519.57 406.32 2.92 WO 2008/139293 PCT/IB2008/001125 -143- 163 L5»cJ S\-l 6-[(3,4-trans)-1-(3-chloro- 2-fluorobenzyl)-4-methy!pyrrolidin-3-yl]-1 -cyclopentyl-1,5-dihydro-4H-pyrazolo[3.4-d]pyrimidin-4-one 53.4 nM 429.173 543.951 430.22 2.85 164 •CY-i XT b 1-cyclopentyl-6-[(3,4-trans)-1-(2,3-dichlorobenzyl)-4-methy1pyrrolidin-3-yl)-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one 78.9 nM 445.144 560.406 446.15 2.94 165 :R»;i ?~0 V>" 1 -cyclopentyl-6-[(3.4-trans)-4-methyl-1-(1,3- thiazol-2-ylmethyl)pyrrolidin-3-yl]- 1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one 54.2 nM 384.173 498.53 385.22 2.35 166 :R*cj b 1 -cyclopentyl-6-[(3,4-trans)-1-(3-fluoro-2-methylbenzyl)-4-methylpyrrolidin-3-yl]-1.5-dihydro-4H-pyrazolc[3,4-d]pynmidin-4-one 68.3 nM 409.228 523.533 410.26 2.83 167 ,?y. f .i 1-cyclopentyl-6-{(3,4-trans)-4-methyl-1-[(2-methylpyrimidin-5-yl)methyl]pyrrolidin-3-yl}- 1 .S-dihydro^H-pyrazolo[3,4-d]pyrimidin-4-one 40.7 nM 393.228 507.519 394.3 2.21 WO 2008/139293 PCT/IB2008/001125 -144- 168 IL'.J,' v_,; Y_/~/ ^ w 1 -cyclopentyl-6-{(3.4-lrans)-1-[(2-ethylpyrimidin-5-yl)methyl]-4-methylpyrrolidin-3-yl}-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidln-4-one 30.7 nM 407.243 521.546 408.31 2.36 169 His. * /V v-Kb 1-cyctopentyl-6-[(3,4- trans)-1-(4-isopropylbenzyl)-4-methylpyrrolidin-3-ylJ-1,5-dlhydro-4H-pyrazolo[3,4-d]pyrimidin-4-one 117 nM 419.269 533.597 420.34 3.1 170 /" v 9 .fY1 1-cyclopentyl-6-{(3,4-trans)-1 -[(1 -ethyl-1H -pyrazol-4-yl)methyl)-4-methylpyrrolidin-3-yl}-1,5-dihydro-4H-pyrazolo[3,4-d)pyrimidin-4-one 74.6 nM 395.243 509.535 396.31 2.37 171 LR*c,' s. b ^ i ; i 1 -cyclopentyl-6-{(3,4-trans)-1-[(4-methoxypyridin-3-yl)methyl]-4-methylpyrrolid in-3-yl}-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one 51.3 nM 408.227 636.555 409.29 2.05 172 >-0 1-cyclopentyl-6-[(3.4-trans)-1-(isoxazol-5-ylmethyl)-4-methylpyrrolidin-3-ylJ-1.5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one 67.0 nM 368.196 482.465 369.25 2.29 WO 2008/139293 PCT/IB2008/001125 -145- 173 •i v 1-cyclopentyl-6-[(3.4-trans)-1-(4-ethoxybenzyl)-4-methylpyrrolidin-3-yl]-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one 64.0 nM 421.248 535.569 422.29 2.85 174 [Kw.' /M V \sx 1-cyclopentyl-6-[(3,4-trans)-1 -{[6-(1 -hydroxy-1 -methylethyl)pyridin-3-yl]methyl}-4-methy1pyrrolidin-3-yl]-1,5-dihydro-4H-pyrazolo[3.4-d]pyrimidin-4-one 42.0 nM 436.259 664.609 437.32 2.14 175 v>>S V"- ^ 1-cyclopentyl-6-{(3,4-trans)-1 -[(2,2-dimethyl- 2,3-dihydro-1-benzofuran-5-yl)methyl]-4-methylpyrrolidin-3-yl}- 1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one 74.8 nM 447.264 561.607 448.3 2.96 176 ■Hii \ y~ \ v_^- / \ 1-cyclopentyl-6-[(3,4- trans>-1 -(3,4-dimethoxybenzyl)-4-methy1pyrrolidin-3-yl]-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one 27.1 nM 437.243 551.568 438.29 2.59 177 [Rac, >0 » W/ \— y~( o \,/ 1-eyclopentyl-6-{(3,4-trans )-4-methyl-1 -[(5-methylpyrazin-2-yl)methyl]pyrrolidin-3-yl>- 1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one 42.2 nM 393.228 507.519 394.3 2.32 178 lit. ^0 V-^"7 Nr~\ 9 ^ fl~ \ -i! W~" 1-cyclopentyl-6-[(3,4-trans)-1-(imidazo[1,2-a]pyridin-2-ylmethyl)-4-methylpyrrolidin-3-yl]-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one J 44.7 nM 417.228 531.541 418.27 2.14 WO 2008/139293 PCT/IB2008/001125 -146- 179 V~\ x/ cnr 1-cyc!opentyl-6-{(3,4-trans)-4-methyl-1-[(2-phenyl-1,3-oxazol-4-yl)methyl]pyrrolidin-3-yl}- 1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one 47.2 nM 444.227 558.563 445.27 2.88 180 o 1 -cyclopentyl-6-[(3,4-trans)-4-methyl-1-(2-methylbenzyl)pyrrolidin-. 3-yl]-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one 83.8 nM 391.237 505.543 392.3 2.77 181 v vpb 1-cyclopentyl-6-[(3.4-trans)-1-(2- isopropoxybenzyl)-4- methylpyrrolidin-3-yl]-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one 134 nM 435.264 549.596 436.33 3 182 LSss.1 >-p \ .f* y~\ V 6-[(3,4-trans}-1 -(cinnolin- 3-ylmethyl)-4-methylpyrrolidin-3-yl]-1 -cyclopentyl-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one 22.5 nM 429.228 543.552 430.26 2.53 183 y>> A V rv 1-cyclopentyl-6-{(3,4-trans)-1-[3-(difluoromethoxy)benzyl]-4-methylpyrrolidin-3-yl}- 1,5-dihydro-4H-pyrazolo[3,4-d)pyrimidin-4-one 29.8 nM 443.213 557.522 444.26 2.87 WO 2008/139293 PCT/IB2008/001125 -147- 184 .R«C. >V\ \_V"Va i ^ ! |j i 1-cyclopentyl-6-[(3,4-trans)-1-(4-fluoro-3-methylbenzyl)-4-methylpyrrolidin-3-yl]-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one 90.0 nM 409.228 523.533 410.26 2.87 185 vy o 1-cyclopentyl-6-{(3,4-trans)-4-methyf-1-[4-(1H- pyrazol-1-yl)benzyl]pyrro[idin-3-yl}- 1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one 33.5 nM 443.243 557.579 444.27 2.69 186 ; R.y. V-P b v 1-cyclopentyl-6-{(3,4- trans}-1-[(2,7-dimethylimidazo[1.2-a]pyridin-3-yl)methyl]-4-methylpyrrolidin-3-yl)-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one 155 nM 445.259 559.595 446.27 2.19 187 lR»C; }-/"S . Wpv, b ° "Yy1 j 1 -cyclopentyl-6-[(3.4-trans}-1-(3,5-dichlorobenzy!)-4-m ethylpyrrolidin-3-y I]-1,5-dihydro-4H-pyrazolo[3.4-d]pyrimidin-4-one 70.9 nM 445.144 560.406 446.16 3.04 188 iRre' bCL b"* ° 1 -cyclopentyl-6-[(3,4- trans)-1-(4-isopropoxybenzyl)-4-methylpyrrolidin-3-yl]-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one 72.7 nM 435.264 549.596 436.3 2.98 WO 2008/139293 PCT/IB2008/001125 -148- 189 \ n o ^ 1-cyclopentyl-6-[(3,4-trans)-1 -{[2-(1 -hydroxy-1 -methylethyl)pyridin-4-yl]methyl}-4-methy1pyrrolidin-3-yl]-1,5-dihydro-4H-pyrazolo[3,4-dJpyrimidin-4-one 23.7 nM 436.259 664.609 437.21 2.13 190 y i vf-^b 1 -cyclopentyk6-[(3.4-trans)-4-m6thyl-1-(4,5,6,7-tetrahydro-1,3- benzothiazol-2-ylmethyl)pyrrolidin-3-yl]- 1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one 55.4 nM 438.22 552.622 439.27 2.81 191 Y-/X, (yj \-.y* Y\ O ^ i I1 1 -cyclopentyl-6-[(3,4-trans>-1-(mesitylmethyl)-4-methy1pyrrolidin-3-yl]- 1,5-dihydro-4H-pyrazolo[3.4-d]pyrimidin-4-one 19S nM 419.269 533.597 420.34 3 192 [Rac] V_/v <C\-i y* r i ex. 1 -cyclopentyl-6-[(3,4-trans)-1-(2,6-dichlorobenzy1)-4-methylpyrrolidin-3-yl]-1.5-dihydro-4H-pyrazolo[3,4-djpyrimidi n-4-one 236 nM 445.144 560.406 446.14 2.75 193 IWwc V~( o v,y 4-{[(3,4-trans)-3-(1-cydopentyl-4-OXO-4,5-dihydro-1 H-pyrazolo[3,4-d]pyrimidin-6-yl)-4-methylpyrrolidin-1-yl]methyl}benzonitrile 57.7 nM 402.217 516.526 403.25 2.61 WO 2008/139293 PCT/IB2008/001125 -149- 194 yp 0 ^ 1 -cyclopentyl-6-[(3,4-trans)-1-(2-fluoro-5-methoxybenzyl)-4-methy1pyrrolidin-3-yl}-1.5-dihydro-4H-pyrazo!o[3,4-d]pyrimidin-4-one 54.7 nM 425.223 539.532 426.29 2.75 195 A is 1 -cyclopentyl-6-[(3.4- trans)-1-(2p6- dimethyfbenzyl)-4-methylpyrrolidin-3-ylJ-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one 198 nM 405.253 519.57 406.31 2.83 196 LR»e. y~i^r v y 1-cyclopentyl-6-((3,4-trans)-1-{(4-methoxy-3,5-dimethylpyridin-2-yl)methyl]-4-methy lpyrrolidin-3-yl}-1,5-dihydro-4H-pyrazolo[3.4-d)pyrimidin-4-one 187 nM 436.259 664.609 437.32 2.76 197 vSacj 0 ° 1 II ^ 1-cyclopentyl-6-[(3.4- trans)-1 -(3,5-dimethy1benzyl)-4-me!hylpyrrolidin-3-yl]-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one 53.5 nM 405.253 519.57 406.33 2.96 198 VR«:, {\ J y i 1-cyclopentyl-6-[(3,4- tra ns)-1 -(3,4-dimethy1benzyl}-4-methylpyrrolidin-3-y l]-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one 93.6 nM 405.253 519.57 406,31 2.94 WO 2008/139293 PCT/IB2008/001125 -150- 199 '.K»c! "\_ y~\ V ° Cpc 1-cyclopentyl-6-{(3,4-trans)-4-methyl-1-[(1-methyMH-benzimidazol-2-yl)methyl]pyrrolidin-3- yl)-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one 35.3 nM 431.243 545.568 432.29 2.64 200 1-cyclopentyl-6-{(3,4-transM-methyl-1-[(4-methyl-3,4-dihydro-2H- 1,4-benzoxazin-7-yl)methyl]pyrrolidin-3-yl)- 1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one 176 nM 448.259 676.62 {449.3} {2.78} 201 1-cyclopentyl-6-[(3,4-trans)-4-methyl-1-(3-pheny1propyt)pyrrolidin-3- ylJ-1,5-dihydro-4 H-pyrazolo[3,4-d]pyrimidin-4-one 146 nM 405.253 519.57 {406.3} {2.93} 202 irjc; kJbo CK 6 A 1-cyclopentyl-6-{(3,4-trans)-4-methyl-1-[2-(trifluoromethyl)benzyl]py rrolidin-3-yl}-1,5-dihydro-4H-pyrazolo[3.4-d]pyrimidin-4-one 104 nM 445.209 559.513 {446.2} {2.9} 203 iBSi' 1-cyclopentyl-6-[(3,4-t rans)—4-methyl-1 -(4,4,4-trifluorobutyf)pyrrolidin-3- yl]-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one 83,7 nM 397.209 511.469 {398.2} {2.69} 204 .Rac: Ax> _ VJ A G^' ° / 1-cyclopentyl-6-[(3.4- trans)-1-(3-methoxybenzyl)-4-methylpyrrolidin-3-yl]-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one 9.56 nM 407.232 521.542 {408.3} {2.74} WO 2008/139293 PCT/IB2008/001125 -151- 205 iRatJ o> c 1 -cyclopentyl-6-[(3,4- trans)-1-(cyclopentylmethyl)-4-methy1pyrrolidin-3-yl]-1.5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one 286 nM 369.253 483.537 {370.2} {2.72} 206 \ 1 -cyclopentyl-6-[(3,4- trans)-1 -(2,4-dimethoxybenzyl)-4-methy1pyrrolidin-3-yl]-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one 68.4 nM 437.243 551.568 {438.2} {2.82} 207 1 yX.X-> O -x '-J h. 1-cyclopentyl-6-{(3.4-trans)-4-methyl-1-[4-(morpholin-4-ylmethyl )benzyl]pyrrolid in -3-yl}-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one 46,2 nM 476.29 590.649 {477.3; 477.3} 41.94;2.1 7} 208 6-[(3.4-trans)-1 -(2,1.3-benzothiadiazol-5-ylmethyl)-4-methylpy nolidin-3-yl]-1 -cyclopentyl-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one 7.13 nM 435.184 549.578 {436,2} {2.66} 209 L54S1 A a 6-{(3.4-trans)-1-[2-(benzyloxy)ethylJ-4-methylpyrrolidin-3-yl)-1 -cyclopentyl-1.5-dihydro-4H-pyrazolo[3.4-d]pyrimidin-4-one 52.9 nM 421.248 535.569 {422.2} {2.85} 210 [Rac. •P-v ^ J\ 1-cyclopentyl-6-[(3.4-trans>-1-(2.6-difluorobenzyl)-4-methylpyrrolidin-3-yl]-1.5-dthydro-4H-pyrazolo[3,4-d]pyrimidin-4-one 16.2 nM 413.203 527.496 {414.2} {2.62} WO 2008/139293 PCT/IB2008/001125 -152- 211 .3* k^i Q"' ^ /" 1 -cyclopentyl-6-[(3.4- trans)-1-(2-methoxybenzyl)-4-methytpyrrolidin-3-yl]-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one 39.8 nM 407.232 521.542 {408.2} {2.76} 212 ^jip- rU" O 'V- 1-cyclopentyl-6-{(3,4-trans)-4-methyl-1 -{(3,5,6- trimethylpyrazin-2-yl)m ethyl] pyrrol idin-3-yl}- 1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one 9.98 nM 421.259 535.573 {422.3} {2.51} 213 LHae yio -O- 0 \. 1-cyclopentyl-6-[(3,4-trans)-1-(2,4-dichlorobenzyl)-4-methy1pyrrolidin-3-yl]-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one 124 nM 445.144 560.406 {446.1} {2.98} 214 LB*tl 9 XX/ \.-J /K ,aj' (.1 d 1-cyclopentyl-6-[(3.4-trans)-4-methyl-1-(5.6.7,8-tetrahydro-4H-pyrazolo[1,5-a]azepin-3-ylmethyl)pyrrolidin-3-yI]- 1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one 15.7 nM 435.275 549.6 {436.3} {2.59} 215 L?.4£ pX> CP 0 1 -cyclopentyl-6-[(3.4-trans)-1-(3-fluorobenzyl)-4-methy1pyrrolidin-3-yl]- 1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one | 11.5 nM 395.212 509.506 {396.2} {2.73} WO 2008/139293 PCT/IB2008/001125 -153- 216 lUiKJ Qy" 0 1 -cyclopentyl-6-[(3,4-trans)-1 -(2,3-dihydro-1 -benzofuran-5-ytmethyl)-4-methylpyrrolidin-3-yl]- 1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one 17.4 nM 419.232 533.553 {420.2} {2.71} 217 .k*. \_-J cv ° /' 1-cyclopentyl-6-[(3,4-trans)-1-(2-methoxy-5-methylbenzyl)-4-methylpyrroiidin-3-y l]-1,5-dihydro-4H-pyrazolo[3,4-dJpyrimidin-4-one 25.0 nM 421.248 535.569 {422.3} {2.91} 218 .R»C A Q-" ^ 1 -cyclopentyl-6-[(3,4-trans)-1-(2-fiuorobenzyl)-4-methytpyrrolidin-3-yl]- 1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one 15.6 nM 395.212 509.506 {396.2} {2.67} 219 -A, B-[(3,4-trans)-1-(2-chlorobenzyl}-4-methylpyrrolidin-3-yl]-1-cyclopentyl-1,5-dihydro-4H-pyrazolo[3.4-d]pyrimidin-4-one 48.4 nM 411.183 525.961 {412.2} {2.78} 220 [Raci kj6o ~r>> ^ 1-cyclopentyl-6-[(3,4-trans)-1 -(3,4-dichlorobenzyl)-4-methy)pyrrolidin-3-yl}-1.5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one 28.0 nM 445.144 560.406 {446.1} {3.04} 221 .R«c, ,... C \j 6-[(3.4-trans)-1 -(2,1,3-benzothiadiazol-4-ylmethyl)-4-methylpyrrolidin-3-yl]-1-cyclopentyl-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one 12.7 nM 435.184 549.578 {436.2} {2.67} WO 2008/139293 PCT/IB2008/001125 -154- 222 -A M-..C /—'V-/'" 1 -cyclopentyl-6-{(3.4-trans )-4-methyl-1 -[(2-propylpyrimidin-5-yl )methyl]pyrrolidin-3-yl}- 1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one 6.03 nM 421.259 535.573 {422.3} {2.52} 223 i,Rae! b&t rO" a L / \ / 1 -cyclopentyl-6-{(3,4-trans)-1 -[(1 -ethyl-1H-pyrazol-5-yl)methyl]-4-methylpyrrolid in-3-yl}-1,5-dihydro-4H-pyrazolo^^-djpyrimidin-4-one 14.4 nM 395.243 509.535 {396.3} {2.39} 224 ;Rie, MA QX" ^ 1-cyc!opentyl-6-{(3,4-trans)-4-methyl-1-[2-(trifluoromethoxy)benzyi] pyrrolidin-3-yl}-1,5-dihydro-4H-pyrazolo[3,4-d]pyrlmidin-4-one 116 nM 461.204 575.512 {462.2} {2.96} 225 ;.x!x> /'A '"A-A v"" 1-cyclopentyl-6-{(3,4-trans)-4-methyl-1-[4-(trifluoromethyl)benzyl]py rrolidin-3-yl}-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one 34.9 nM 445.209 559.513 {446.2} {3.01} 226 1-cyclopentyl-6-{{3.4-trans)-4-methyl-1-[(1-methyl-1 H-imidazo[*5- c]pyridin-2-yl)methy1]pyrrolidin-3-yl}- 1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidln-4-one 3.78 nM 432.239 660.581 {433.2} {2.03} 227 Rmz, i rV. W """ 1-cyclopentyl-6-[(3.4-trans)-1-(3,5-difluorobenzyl)-4-methylpyrrolidin-3-yl]-1.5-dihydro-4H-pyrazolo[3.4-d]pyrlmidin-4-one 7.42 nM 413.203 527.496 {414.2} {2.79} </div>

Claims

<div class="application article clearfix printTableText" id="claims"> WO 2008/139293 PCT/IB2008/001125 -155- 228 .Rac; c> 1-cyclopentyl-6-[(3,4- xV) trans)-1-(5,6-dihydro-4H- pyrrolo[1,2-b]pyrazoi-3- ylmethyl)-4- 49.1 nM 407.243 521.546 {408.3} {2.37} CP \ ! methytpyrrolidin-3-yl]-1,5- <J dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one Although certain presently preferred embodiments of the invention have been described herein, it will be apparent to those skilled in the art to which the invention pertains that variations and modifications of the described 5 embodiments may be made without departing from the spirit and scope of the invention. Accordingly, it is intended that the invention be limited only to the extent required by the appended claims and the applicable rules of law. WO 2008/139293 PCT/IB2008/001125 -156-CLAIMS We claim:

1. A compound of Formula (I), (I) or a pharmaceutically acceptable salt thereof, wherein: R is selected from the group consisting of (CrCeJalkyl, (C2-C6)alkenyl, (C2-C6)alkynyl, (C3-C8)cycloalkyl, heterocycloalkyl, aryl, and heteroaryl, each 10 of which optionally may be substituted with one to three substituents, the substituents being independently selected from the group consisting of (Cr C4)alkyl, (CrC4)alkoxy, halo, and (CrC4)haloalkyl. R-i is selected from the group consisting of hydrogen, (CrC4)alkyl, (C2-C4)alkenyl, (C2-C4)alkynyl, (Ci-C4)haloalkyl, and cyclopropyl; 15 R2 is selected from the group consisting of (Ci-Ce)alkyl, (C2-C6)alkenyl, (C2-C6)alkynyl, (CrC6)haloalkyl, heteroaryl selected from the group consisting of pyridinyl, pyridazinyl, pyrimidinyl, and pyrazinyl, and ER5, wherein the heteroaryl optionally may be substituted with one to three substituents independently selected from the group consisting of (Ci-C4)alkyl and (Cr 20 C4)haloalkyl; R3 is selected from the group consisting of hydrogen, (CrC4)alkyl, (C2-C4)alkenyl, (C2-C4)alkynyl, (C3-C6)cycloalkyl, and (CrC4)haloalkyl; E is selected from the group consisting of -CH2- -CH2CH2- -CH2CH2CH2-, and -C(O)- ; WO 2008/139293 PCT/IB2008/001125 -157- R5 is selected from the group consisting of (C3-C8)cycloalkyl, heterocycloalkyl, aryl, aryloxy, and heteroaryl, any of which optionally may be substituted with one to three substituents, such substituents being independently selected from the group consisting of (Ci-C4)alkyl, (C2-5 C4)alkenyl, (C2-C4)alkynyl, (CrC4)hydroxyalkyl, (Ci-C4)haloalkyl, (Cr C4)alkoxy, (Ci-C4)haloalkoxy, (C3-C8)cycloalkyl, halo, cyano, phenyl, morpholinyl, (Ci-C4)alkylamino, pyrazolyl, triazolyl, and imidazolyl.

2. The compound of claim 1, or a pharmaceutically acceptable salt thereof, 10 wherein: R is selected from the group consisting of ethyl, isopropyl, trifluoroethyl, cyclobutyl, cyclopentyl, difluorocyclohexyl, methoxyphenyl, tetrahydro-2H-thiopyran-4-yl, and tetrahydro-2H-pyran-4-yl; Ri is hydrogen or methyl; 15 R2 is methyl, trifluoroethyl, trifluorobutyl, pyrimidinyl, trifluoromethylpyrimidinyl, or ER5; R3 is methyl, ethyl, isopropyl, trifluoromethyl, trifluoroethyl, or cyclopropyl; E is -CH2- or -C(O)- ; 20 R5 is selected from the group consisting of substituted or unsubstituted cyclopentyl, morpholinyl, phenyl, naphthyl, benzyloxy, pyrimidinyl, pyridinyl, quinolinyl, quinoxalinyl, pyrazinyl, pyrazolyl, benzimidazolyl, cinnolinyl, naphthydrinyl, pyrido[2,3-b]pyrazinyl, imidazo[4,5-c]pyridinyl, benzothiadiazolyl, tetrahydropyrazolo[1,5-a]pyridinyl, dihydrobenzodioxinyl, 25 imidazolyl, dihydrobenzofuranyl, triazolyl, oxazolyl, isoxazolyl, benzodioxinyl, thiazolyl, imidazo[1,2-a]pyridinyl, tetrahydrobenzothiazolyl, dihydrobenzoxazinyl, tetrahydropyranyl, tetrahydropyrazolo[1,5-a]azepinyl, and dihydropyrrolo[1,2-b]pyrazolyl. 30

3. The compound of claim 2, or a pharmaceutically acceptable salt thereof, wherein: WO 2008/139293 PCT/IB2008/001125 -158- R is selected from the group consisting of isopropyl, cyclobutyl, cyclopentyl, and tetrahydro-2H-pyranyl; Ri is hydrogen; R2 is ER5; R3 is methyl or ethyl; E is -CH2-; and R5 is selected from the group consisting of phenyl, pyrimidin-2-yl, pyridin-2-yl, pyrazin-2-yl, and 5-methylpyrazin-2-yl.

4. The compound of claim 1, selected from the group consisting of: 6-[(3S,4S)1 -benzyl-4-methylpyrrolidin-3-yl]-1 -cyclopentyl-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 1-cyclopentyl-6-[(3S,4S)-4-methyl-1-(quinoxalin-6-ylmethyl)pyrrolidin-3-yl]-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 1 -cyclopentyl-6-{(3S,4S)-4-methyl-1 -[(5-methylpyrazin-2-yl)methyl]pyrrolidin-3-yl}-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 1-cyclopentyl-6-{(3S,4S)-1-[(1,3-dimethyl-1H-pyrazol-5-yl)methyl]-4-methylpyrrolidin-3-yl}-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 1-cyclopentyl-6-[(3S,4S)-4-methyl-1-(4,5,6,7-tetrahydropyrazolo[1,5-a]pyridin-3-yimethyl)pyrrolidin-3-yi]-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 1-cyclopentyl-6-{(3S,4S)-4-methyl-1-[(1 -methyl-1 H-benzimidazol-2-yl)methyl]pyrrolidin-3-yl}-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 6-[(3S,4S)-1-(cinnolin-3-ylmethyl)-4-methylpyrrolidin-3-yl]-1-cyclopentyl-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 1-cyclopentyl-6-{(3S,4S)-4-methyl-1-[(2-rnethylpyrimidin-4-yl)methyl]pyrrolidin-3-yl}-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; and 1-cyclopentyl-6-[(3S,4S)-1-{[2-(dimethylamino)pyrimidin-4-yl]methyl}-4-methylpyrrolidin-3-yl]-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; or a pharmaceutically acceptable salt thereof. WO 2008/139293 PCT/IB2008/001125 -159-

5. The compound of claim 1, selected from the group consisting of: 6-[(3S,4S)-1 -benzyl-4-ethylpyrrolidin-3-yl]-1 -isopropyl-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 6-[(3S,4S)-1 -benzyl-4-methylpyrrolidin-3-yl]-1 -isopropyl-1,5-dihydro-5 4H-pyrazolo[3,4-d]pyrimidin-4-one; 1-isopropyl-6-[(3S,4S)-4-methyl-1-(quinoxalin-6-ylmethyl)pyrrolidin-3-yl]-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 1-isopropyl-6-[(3S,4S)-4-methyl-1-(quinolin-3-ylmethyl)pyrrolidin-3-yl]-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 10 1 -isopropyl-6-[(3S,4S)-4-methyl-1 -(quinoxalin-6-ylmethyl)pyrrolidin-3- yl]-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 1-isopropyl-6-[(3S,4S)-4-methyl-1-(quinolin-3-ylmethyl)pyrrolidin-3-yl]-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 1 -isopropyl-6-{(3S,4S)-4-methyl-1 -[(2-methylpyrimidin-5-15 yl)methyl]pyrrolidin-3-yl}-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 1 -isopropyl-6-{(3S,4S)-1 -[(6-methoxypyridin-3-yl)methyl]-4-methylpyrrolidin-3-yl}-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 1 -isopropyl-6-{(3S,4S)-4-methyl-1 -[(5-methylpyrazin-2-yl)methyl]pyrrolidin-3-yl}-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 20 1-isopropyl-6-[(3S,4S)-4-methyl-1-(1,5-naphthyridin-4- ylmethyl)pyrrolidin-3-yl]-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 1-isopropyl-6-[(3S,4S)-4-methyl-1-(1,8-naphthyridin-4-ylmethyl)pyrrolidin-3-yl]-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 1-isopropyl-6-[3S,4S)-4-methyl-1-(quinolin-4-ylmethyl)pyrrolidin-3-yl]-25 1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; and 1 -isopropyl-6-[(3S,4S)-4-methyl-1 -(pyrido[2,3-b]pyrazin-8-ylmethyl)pyrrolidin-3-yl]-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; or a pharmaceutically acceptable salt thereof. 30

6. The compound of claim 1, selected from the group consisting of: WO 2008/139293 PCT/IB2008/001125 -160- 6-[(3S,4S)-1 -benzyl-4-methylpyrrolidin-3-yl]-1 -(tetrahydro-2H-pyran-4-yl)-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 6-{(3S,4S)-4-methyl-1-[(2-methylpyrimidin-5-yl)methyl]pyrrolidin-3-yl}-1-(tetrahydro-2H-pyran-4-yl)-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 5 6-{(3S,4S)-4-methyl-1 -[(5-methylpyrazin-2-yl)methyl]pyrrolidin-3-yl}-1 - (tetrahydro-2H-pyran-4-yl)-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 6-{(3S,4S)-1-[(6-methoxypyridin-3-yl)methyl]-4-methylpyrrolidin-3-yl}-1-(tetrahydro-2H-pyran-4-yl)-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 6-[(3S,4S)-4-methyl-1-(quinolin-3-ylmethyl)pyrrolidin-3-yl]-1-(tetrahydro-10 2H-pyran-4-yl)-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 6-{(3S,4S)-4-methyl-1-[(2-methylpyrimidin-4-yl)methyl]pyrrolidin-3-yl}-1-(tetrahydro-2H-pyran-4-yl)-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 6-{(3S,4S)-4-methyl-1-[(6-rnethylpyridin-3-yl)methyl]pyrrolidin-3-yl}-1-(tetrahydro-2H-pyran-4-yl)-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 15 6-[(3S,4S)-4-methyl-1-{[6-(trifluoromethyl)pyridin-3-yl]methyl}pyrrolidin- 3-yl]-1-(tetrahydro-2H-pyran-4-yl)-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 6-{(3S,4S)-4-methyl-1 -[(1 -methyl-1 H-imidazo[4,5-c]pyridin-2-yl)methyl]pyrrolidin-3-yl}-1-(tetrahydro-2H-pyran-4-yl)-1,5-dihydro-4H-20 pyrazolo[3,4-d]pyrimidin-4-one; 6-{(3S,4S)-1-[(1,3-dimethyl-1H-pyrazol-5-yl)methyl]-4-methylpyrrolidin-3-yl}-1 -(tetrahydro-2H-pyran-4-yl)-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 6-[(3S,4S)-1-(2,1,3-benzothiadiazol-5-ylmethyl)-4-methylpyrrolidin-3-25 yl]-1-(tetrahydro-2H-pyran-4-yl)-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 6-[(3S,4S)-4-methyl-1-(quinoxalin-2-ylmethyl)pyrrolidin-3-yl]-1-(tetrahydro-2H-pyran-4-yl)-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 6-[(3S,4S)-4-methyl-1-(quinolin-4-ylmethyl)pyrrolidin-3-yl]-1-(tetrahydro-30 2H-pyran-4-yl)-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; WO 2008/139293 PCT/IB2008/001125 -161- 6-[(3Sl4S)-4-methyl-1-(pyridin-2-ylmethyl)pyrrolidin-3-yl]-1-(tetrahydro-2H-pyran-4-yl)-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 6-[(3S,4S)-1-benzyl-4-methylpyrrolidin-3-yl]-3-methyl-1-(tetrahydro-2H-pyran-4-yl)-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 5 6-[(3S,4S)-1 -(3-fluorobenzyl)-4-methylpyrrolidin-3-yl]-1 -(tetrahydro-2H- pyran-4-yl)-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 6-[(3S,4S)-1-(35-difluorobenzyl)-4-methylpyrrolidin-3-yl]-1-(tetrahydro-2H-pyran-4-yl)-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 6-{(3S,4S)-4-methyl-1-[4-(trifluoromethyl)benzyl]pyrrolidin-3-yl}-1-10 (tetrahydro-2H-pyran-4-yl)-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 3-methyl-6-[(3S,4S)-4-methyl-1-(pyridin-3-ylmethyl)pyrrolidin-3-yl]-1-(tetrahydro-2H-pyran-4-yl)-1J5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 3-methyl-6-{(3S,4S)-4-methyl-1 -[(2-methylpyrimidin-5-yl)methyl]pyrrolidin-3-yl}-1-(tetrahydro-2H-pyran-4-yl)-1,5-dihydro-4H-15 pyrazolo[3,4-d]pyrimidin-4-one; 6-{(3S,4S)-1-[(6-methoxypyridin-3-yl)methyl]-4-methylpyrrolidin-3-yl}-3-methyl-1-(tetrahydro-2H-pyran-4-yl)-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 6-{(3S,4S)-4-methyl-1-[(6-methylpyridin-2-yl)methyl]pyrrolidin-3-yl}-1-20 (tetrahydro-2H-pyran-4-yl)-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 6-[(3S,4S)-1-(4-fluorobenzyl)-4-methylpyrrolidin-3-yl]-1-(tetrahydro-2H-pyran-4-yl)-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 6-[(3S,4S)-1-benzyl-4-ethylpyrrolidin-3-yl]-1-(tetrahydro-2H-pyran-4-yl)-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 25 6-[(3S,4S)-1 -(2-fluorobenzyl)-4-methylpyrrolidin-3-yl]-1 -(tetrahydro-2H- pyran-4-yl)-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 6-{(3S,4S)-4-methyl-1-[2-(trifluoromethyl)benzyl]pyrrolidin-3-yl}-1-(tetrahydro-2H-pyran-4-yl)-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 6-[(3S,4S)-1-(2,4-difluorobenzyl)-4-methylpyrrolidin-3-yl]-1-(tetrahydro-30 2H-pyran-4-yl)-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; WO 2008/139293 PCT/IB2008/001125 -162- 6-[(3S,4S)-1-(4-methoxybenzyl)-4-methylpyrrolidin-3-yl]-1-(tetrahydro-2H-pyran-4-yl)-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 6-[(3S,4S)-1-benzyl-4-methylpyrrolidin-3-yl]-1-(tetrahydro-2H-thiopyran-4-yl)-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 5 6-[(3S,4S)-1-(2-methoxybenzyl)-4-methylpyrrolidin-3-yl]-1-(tetrahydro- 2H-pyran-4-yl)-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 6-[(3S,4S)-1-(3-methoxybenzyl)-4-methylpyrrolidin-3-yl]-1-(tetrahydro-2H-pyran-4-yl)-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 6-{(3S,4S)-4-methyl-1-[3-(trifluoromethyl)benzyl]pyrrolidin-3-yl}-1-10 (tetrahydro-2H-pyran-4-yl)-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 6-[(3S,4S)-1-(26-difluorobenzyl)-4-methylpyrrolidin-3-yl]-1-(tetrahydro-2H-pyran-4-yl)-T,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 6-{(3S,4S)-4-ethyl-1-[(5-methylpyrazin-2-yl)methyl]pyrrolidin-3-yl}-1-(tetrahydro-2H-pyran-4-yl)-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 15 6-{(3S,4S)-4-ethyl-1-[(6-methoxypyridin-3-yl)methyl]pyrrolidin-3-yl}-1- (tetrahydro-2H-pyran-4-yl)-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 6-[(3S,4S)-4-ethyl-1-(pyridin-2-ylmethyl)pyrrolidin-3-yl]-1-(tetrahydro-2H-pyran-4-yl)-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 6-[(3S,4S)-4-ethyl-1-(quinoxalin-2-ylcarbonyl)pyrrolidin-3-yl]-1-20 (tetrahydro-2H-pyran-4-yl)-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 6-[(3S,4S)-4-methyl-1-(pyrimidin-2-ylmethyl)pyrrolidin-3-yl]-1-(tetrahydro-2H-pyran-4-yl)-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one; 2-({(3S,4S)-3-ethyl-4-[4-oxo-1-(tetrahydro-2H-pyran-4-yl)-4,5-dihydro-1H-pyrazolo[3,4-d]pyrimidin-6-yl]pyrrolidin-1-yl}methyl)benzonitrile; 25 3-({(3S,4S)-3-ethyl-4-[4-oxo-1-(tetrahydro-2H-pyran-4-yl)-4,5-dihydro- 1 H-pyrazolo[3,4-d]pyrimidin-6-yl]pyrrolidin-1 -yl}methyl)benzonitrile; and 4-({(3S,4S)-3-ethyl-4-[4-oxo-1-(tetrahydro-2H-pyran-4-yl)-4,5-dihydro-1H-pyrazolo[3,4-d]pyrimidin-6-yl]pyrrolidin-1-yl}methyl)benzonitrile; or a pharmaceutically acceptable salt thereof. 30 Received at IPONZ 10 January 2012 -163-

7. A compound of Formula (I), ABS O or a pharmaceutically acceptable salt thereof.

8. A compound which is 5 6-[(3S,4S)-4-methyl-1-(pyridin-2-ylmethyl)pyrrolidin-3-yl]-1-(tetrahydro- 2H-pyran-4-yl)-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one.

9. A pharmaceutical composition comprising a compound of any one of claims 1 to 8, or a pharmaceutically acceptable salt thereof, and a pharmaceutically acceptable vehicle, carrier or diluent. 10

10. The composition of claim 9, further comprising a second pharmaceutical agent.

11. The composition of claim 10, wherein the second pharmaceutical agent is selected from the group consisting of donepezil, galantamine, memantine, rivastigmine, and tacrine. 15

12. Use of a compound of any one of claims 1 to 8, or a pharmaceutically acceptable salt thereof, in the manufacture of a medicament for inhibiting PDE9 in a mammal in need of such inhibition.

13. Use of a compound of any one of claims 1 to 8, or a pharmaceutically acceptable salt thereof, in the manufacture of a medicament for treating a 20 neurodegenerative disease in a mammal in need of such treatment.

14. The use of claim 13, wherein the disease is Alzheimer's disease. Received at IPONZ 10 January 2012 -164-

15. Use of a compound of any one of claims 1 to 8, or a pharmaceutically acceptable salt thereof, in the manufacture of a medicament for promoting neurorestoration in a mammal in need of such neurorestoration.

16. Use of a compound of any one of claims 1 to 8, or a pharmaceutically 5 acceptable salt thereof, in the manufacture of a medicament for improving cognitive deficits in a mammal in need of such improvement.

17. The use of any one of claims 12 to 16 wherein the mammal is human.

18. A compound of formula (I) according to claim 1 and claim 7, substantially as herein described with reference to any one or more of the examples but 10 excluding comparative examples.

19. A compound according to claim 8, substantially as herein described with reference to any one or more of the examples but excluding comparative examples.

20. A pharmaceutical composition, substantially as herein described with 15 reference to any one or more of the examples but excluding comparative examples.

21. Use of a compound according to claim 12, substantially as herein described with reference to any one or more of the examples but excluding comparative examples. 20

22. Use of a compound according to claim 13, substantially as herein described with reference to any one or more of the examples but excluding comparative examples.

23. Use of a compound according to claim 15, substantially as herein described with reference to any one or more of the examples but excluding 25 comparative examples.

24. Use of a compound according to claim 16, substantially as herein described with reference to any one or more of the examples but excluding comparative examples. </div>