NZ525453A

NZ525453A - Near infrared fluorescent contrast agent useful for fluorescence imaging of tumour or in angiography

Info

Publication number: NZ525453A
Application number: NZ525453A
Authority: NZ
Inventors: Naoto Miwa; Michihito Inagaki; Hiroaki Eguchi; Massafumi Kumura; Yoshio Inagaki; Toru Harada
Original assignee: Schering Ag; Fuji Photo Film Co Ltd
Priority date: 1998-09-18
Filing date: 2003-04-23
Publication date: 2003-09-26

Abstract

A near infrared fluorescent contrast agent comprising a sodium salt of the formula shown herein, and a method of fluorescence imaging comprising introducing the near infrared fluorescent contrast agent of the described formula into a living body, exposing the body to an excitation light, and detecting near infrared fluorescence from the contrast agent is disclosed. The near infrared fluorescent contrast agent of the described formula is excited by an excitation light and emits near infrared fluorescence. This infrared fluorescence is superior in transmission through biological tissues. Thus, detection of lesions in the deep part of a living body has been made possible. In addition, this infrared fluorescent contrast agent is superior in water solubility and low toxic, and therefore, it can be used safely for tumour imaging and for angiography.

Description

<div class="application article clearfix" id="description"> 5254 5 3 Patents Form No. 5 N.Z. No. Divided out of Parent Application No. 510019 NEW ZEALAND Patents Act 1953 COMPLETE SPECIFICATION NEAR INFRARED FLUORESCENT CONTRAST AGENT AND FLUORESCENCE IMAGING We, SCHERING AKTIENGESELLSCHAFT, a Germany Company of Mullerstrasse 178, D-13353 Berlin, Germany and FUJI PHOTO FILM CO., LTD., a Japanese Company of 210, Nakanuma, Minamiashigara-shi, Kanagawa 250-0193, Japan, do hereby declare the invention, for which we pray that a patent may be granted to us, and the method by which it is to be performed, to be particularly described in and by the following statement:- -1- (Followed by 1A) W 0/16810 1A PCT/EP99/07088 near infrared fluorescent contrast agent and fluorescence imaging technical field of the invention The present invention relates to a near infrared fluorescent contrast agent and fluorescence imaging using said contrast agent. In treating diseases, it is vital to detect morphological and functional changes caused by the disease in the living body at an early stage of the disease. Especially when treating cancer, the site and size of tumor are significant determinant factors of the effective treatment design. Known methods for this purpose include biopsy by puncture and the like, and imaging diagnoses such as X-ray imaging, MRI, ultrasound imaging and the like. Biopsy is effective for definitive diagnosis, but at the same time it places great burden on test subjects and is not suitable for tracking time-course changes in lesions. X-ray imaging and MRI inevitably expose test subjects to radiation and magnetic waves. In addition, conventional imaging diagnoses as mentioned above require complicated operation and a long time for measurement and diagnosis. A large apparatus used for this purpose also makes it difficult to apply these methods during operation. One of the image diagnoses is fluorescence imaging (Lipspn R. L. et al., J. Natl. Cancer Inst, 26,1-11 (1961)). This method uses, as a contrast agent, a substance that emits fluorescence upon exposure to an excitation light having a specific wavelength. Thus, a body is exposed to an excitation light from outside the body and the fluorescence emitted from the fluorescent contrast agent in the body is detected. Such fluorescent contrast agent may be, for example, a porphyrin compound that accumulates in tumor and is used for photodynamic therapy (PDT), such as haematoporphyrin. Other examples include photophrin and benzoporphyrin WO 00/16810 2 PCk.^P99/07088 (see Lipspn R. L. et al., supra, Meng t. S. et al., SPIE, 1641, 90-98 (1992), WO 84/04665 and the like). These compounds are originally used for PDT and have phototoxicity, since this is what PDT requires. Consequently, these are not desirable diagnostic agents. Meanwhile, retinal circulatory microangiography using a known fluorescent dye, such as fluorescein, fluorescamin and riboflabin, has been known (U.S. Patent No. 4945239). These fluorescent dyes emit fluorescence in a visible light region of 400-600 nm. In this region, the light transmission through living tissue is very low, so that the detection of lesions in the deep part of a body is nearly impossible. in addition, the use, as fluorescent contrast agent, of cyanine compounds inclusive of indocyanine green (hereinafter to be abbreviated as ICG), which are used to determine liver function and cardiac output, has been documented (Haglund M. M. et al., Neurosurgery, 35,930 (1994), Li, X. et al., SPIE, 2389, 789-797 (1995)). Cyanine compounds show absorbance in a near infrared light region (700-1300 nm). Near infrared light shows high transmission through living tissues and can pass through a skull of about 10 cm in size. Because of this, it has been increasingly attracting attention in clinical medicine. For example, optical CT technique using optical transmission of medium has been drawing attention in the clinical field as a new technology. This is because near infrared light can pass through living body and can be used for monitoring oxygen concentration and circulation in the living body. Cyanine compounds emit fluorescence in the near infrared region. The fluorescence in this region can pass through living tissues and offers the potential for a fluorescent contrast agent. Various cyanine compounds have been developed in recent years and tried as fluorescent contrast agents (W096/17628, WP97/13490 and the like). However, an agent having sufficient Vv J0/16810 PCT/EP99/07088 3 solubility in water and safety to living body, as well as capability of distinguishing normal tissues from diseased tissues (selectivity for imaging target site), is not in existence. summary of the invention It is therefore an object of the present invention to provide a fluorescent contrast agent. The inventive agent is low toxic and has a superior solubility in water. In addition, it emits fluorescence in a near infrared region that can pass through living tissues, and permits specific imaging of tumor and/or blood vessel. Another object of the present invention is to provide a method of fluorescence imaging using said near infrared fluorescent contrast agent The present invention is predicated on the finding that introduction of three or more sulfonic add groups into a cyanine dye compound results in the provision of a fluorescent contrast agent having a high solubility in water. It has been also found that a method of fluorescence imaging can be established when this contrast agent is used. The present invention provides the sodium salt of the formula ch3 ch3 Na03S (ch^sof (CH2)2S03Na In a further aspect the present invention provides a near infrared fluorescent contrast agent comprising the above sodium salt. 3A The present invention also provides the following. (1) A near infrared fluorescent contrast agent comprising a compound having three or more sulfonic acid groups in a molecule, which is represented by the formula [I] wherein R1 and R2 are the same or different and each is a substituted or unsubstituted alkyl; Z1 and Z2 are each nonmetallic atoms necessary for forming a substituted or unsubstituted condensed benzo ring or condensed WO 00/16810 4 PCIW99/07088 naphtho ring; r is 0,1 or 2; L1 - L7 are the same or different and each is a substituted or unsubstituted methine, provided that when r is 2, L6 and L7 that occur in duplicate are the same or different; and X and Y are the same or different and each is a group of the formula R3 I — o— , —S— • —CH=CH— or —C— :i4 wherein R3 and R4 are the same or different and each is substituted or unsubstituted alkyl, or a pharmaceutically acceptable salt thereof. (2) The near infrared fluorescent contrast agent of (1) above, which is free of a carboxylic acid group in a molecule. (3) The near infrared fluorescent contrast agent of (1) or (2) above, wherein, in the formula [I], r is 1. (4) The near infrared fluorescent contrast agent of any of (1) to (3) above, wherein 4 or more sulfonic acid groups are contained in a molecule. (5) The near infrared fluorescent contrast agent of any of (1) to (4) above, wherein 10 or less sulfonic acid groups are contained in a molecule. (6) The near infrared fluorescent contrast agent of any of (1) to (4) above, wherein 8 or less sulfonic acid groups are contained in a molecule. (7) The near infrared fluorescent contrast agent of any of (1) to (6) above, wherein the pharmaceutically acceptable salt is a sodium salt. (8) The near infrared fluorescent contrast agent of any of (1) to (7) above, that is for tumor imaging and/or angiography. (9) A sodium salt of a compound of the formula [II] having three or more sulfonic acid groups in a molecule Wo jQ/16810 5 PCT/EP99/07088 wherein R1, R2, L1 - L7, X and Y are as defined above, and R5 to R16 are the same or different and each is a hydrogen atom, a sulfonic acid group, a carboxyl group, a hydroxyl group, an alkyl(suifoalkyl)amino group, a bis(sulfoalkyl)amino group, a sulfoalkoxy group, a (sulfoalkyl)sulfonyl group or a (sulfoalkyl)aminosulfonyl group, exclusive of the groups of the following formulas (CH2)3S03 (CH2)3S03Na (CH2)2CHS03 (CH^CHSC^Na I I ch3 ch3 wo 00/16810 6 pct/£-»>99/07088 (ch2)2chs03 I ch3 (CH2)2CHS03Na CH3 (CH2)2S03 (CH2)2S03Na co2c2h5 (CH2)2S03 (CH2)2S03Na S03Na (ch2)2chs03" ch3 (CH2)2CHS03Na I ch3 Wi. J/16810 7 PCT/EP99/07088 (10) The sodium salt of (9) above, wherein, in the formula [II], R1 and R2are each a lower alkyl having 1 to 5 carbon atoms substituted by a sulfonic acid R17 I — c — group and X and Y are the same or different and each is a group of the formula wherein R17 and R18 are unsubstituted lower alkyl having 1 to 5 carbon atoms. (11) The sodium salt of (10) above, having the formula (CH2)4S03~ (GH2)4S03Na (12) A sodium salt of a compound of the formula [111-1] having three or more sulfonic acid groups in a molecule wherein L1-L7 are as defined above, R19 and R20 are lower alkyl having 1 to 5 carbon atoms and substituted by sulfonic acid group, R21 - R28 are the same or different and each is hydrogen atom, sulfonic acid group, carboxy! group, hydroxyl group, alkyl(sulfoalkyl)amino group, bis(sulfoalkyl)amino group, WO 00/16810 8 PCT,~*»99/07088 R17 I — c — I R18 sulfoalkoxy group, (sulfoalkyi)sulfonyl group or (sulfoalkyl)aminosulfonyl group, and X' and V are the same or different and each is a group of the formula wherein R17 and R18 are as defined above, exclusive of the groups of the following formulas (CH2)4S03' (CH2)4S03Na Na03S, ch3 ch3 C2H5 C2H5 ch-ch S03Na (CH2)4S03Na Na03SCH2CH2NH02S, ch3 ch3 ch3 ch3- N+ CH^CH-CH^ (CH2)4S03- & S02NHCH2CH2S03Na (CH2)4S03Na W. J0/16810 9 PCT/EP99/07088 N I \ (ch2)4S03 (ch2)4s03k ^ CH^CH-CH^N' (ch2)2s03" (ch2)2s03k Na03sch202S, ch3 ch3 ch3 ch3- CH^CH-CH^N' (ch2)2s03k N (ch2)2s03 and so2ch2ch2so3k (13) The sodium salt of (12) above, wherein, in the formula [111-1], U is methine substituted by alkyl having 1 to 4 carbon atoms. (14) The sodium salt of (12) above, which is a sodium salt of the compound of the formula [III-2] having three or more sulfonic acid groups in a molecule [III-2] wherein R19 - R28, X* and Y* are as defined above, Z3 is a non-metallic atom group necessary for forming a 5- or 6-membered ring and A is hydrogen atom WO 00/16810 10 PCT/»ji'99/07088 or a monovalent group. (15) The sodium salt of (14) above, having the formula Na03S (CH2)4S<V SOiNa (CH2)4S03Na (16) The sodium salt of (12) above, having the formula SO-»Na I (CH:)2S05 (CH2)2S03Na (17) The sodium salt of any of (9), (10), (12), (13) and (14) above, comprising 4 or more sulfonic acid groups in a molecule. (18) The sodium salt of any of (9), (10), (12), (13), (14) and (17) above, comprising 10 or less sulfonic acid groups in a molecule. (19) The sodium salt of any of (9), (10), (12), (13), (14) and (17) above, comprising 8 or less sulfonic acid groups in a molecule. (20) A near infrared fluorescent contrast agent comprising the sodium salt of any of (9) to (19) above. (21) The near infrared fluorescent contrast agent of (20) above, which is for tumor imaging and/or angiography. (22) A method of fluorescence imaging comprising introducing the near infrared fluorescent contrast agent of (1) above into a living body, exposing the body to an excitation light, and detecting near infrared fluorescence from the contrast agent. (23) The sodium salt of (9) above, which is at least one member selected from the group consisting of the compounds of the following formulas WC .0/16810 11 PCT/EP99/07088 NaO,S NaO,S SO,Na CH, NaO?S. CH, CH, CH, +^CH=^CH-CH^N SO,Na CH2CH2CHS03 CH2CH2CHS03Na CH, CH, CH, CH, CH3 V.«3 CH=^=CH-CH^n SO,Na (CH2)4S03~ (CH2)4S03Na CH, CH, ch3 ^xl3 ch=f= ch—ch^f^n SO,Na (ch2)4so3- (CH2)4S03Na (CH2)4S03 (CH2)4S03Na WO 00/16810 12 PCl#~P99/07088 and Wo 00/16810 13 PCT/EP99/07088 NaOjS S0,Na CH, CH, CI CH=CH-j^^ = SOjNa CH-CH'^N I (CH2)4S0J (CH2)4S03Na (24) The sodium salt of (12) above, which is at least one member selected from the group consisting of the compounds of the following formulas :h3 ch3 ch3 CH=^CH-CH^n SO,Na (CH^SOf (CH2>2S03Na I (CH2)4S03 (CH2)4S03Na NaOjS CH3 CH, CI I ch=ch-ch=ch-c=ch-ch S03Na (CH2)4S03- (CH2)4S03Na -14- 10 CH3 ch3 ch3 I CH=CH-CH=C-CH=CH-CH I (CH2)4S03 S03Na (CH2)4S03Na 15 20 Na03s- S03Na C,H 2 5. NaOjSCCH^/ (ch2)2s03Na CH, CH: ^%f^CH=(CH-CH)<^Ijr (CH^SO/ \ (CH2)3S03Na Na03S- 25 ch=^= ch — ch^p" n S03Na (CH2)3S03 (CH2)3S03Na -15- Na03S(CH2)4 CH=f=CH-CH^N (ch2)4so3" (CH2)4S03Na (CH2)4S03Na 10 (afe)4sp3 (Ctfc)4S03Na 15 20 (CH2)3S03 (CH2)3S03Na Wo 00/16810 16 PCT/EP99/07088 S03Na CH3 CH, ch, ch, SOiNa N*^CH=^ CH—CH^n Na03S (CH2)4S03 | S03Na (CH2)4S03Na Na03SCH2CH202S CH, CH, ^Sr^CH-f= CH— CH^n SC^CHjCHzSC^Na I (CH^SOj" (CH2)2S03Na CH, Na03SCH2CH2CH20v/% CH, CH, CH, ^N^CH=f CH—CH^N' I I ■ 0CH2CH2CH2S03Na (CH2)jSOr (CH^SOaNa NaOOC CH, CH3 CH3 CH=f= CH— CH^f^N SOiNa (CH2)3S03 (CH2)3S03Na Wo 00/16810 17 PCT/EP99/07088 CH, (Na03SCH2CH2CH2)2N, CH, CH, CH, Jr^CH^ CH—CH^N (CH2)3S03- N(CH2CH2CH2S03Na)2 (C^JjSOjNa NaO,S CH, CH, CH3 ch3 CH^XiJS^ CH=CH-CH N (CH2)4S03- SO,Na (CH2)4S03Na (CH2)4S03" (CH^SOjNa NaOjS CH2CH2S03Na i ch3 CH=CH-CH=C-CH=CH-CH SO,Na I (CH2)4S03- (CH2)4S03Na WO 00/16810 18 PCT/El'99/07088 SO,Na (ch2)4so3- (CH2)4S03Na NaC^S ch 3 SCH2CH2S03Na CH3 CH3 \ CH3 CH=CH I (CH2)4S03- SOiNa CH-CH*^N (CH2)4S03Na NaOjS ch, CH3 (ch2)4so3~ S03Na CH=CH-^^j= CH-CH^^N' S03Na I (CH2)4S03Na Wv. J0/16810 19 pct/ep99/07088 NaOjS S03Na ch, CH, ch=ch-|^^j= (ch2)4so3- SO,Na ch-CH^^N (CH2)4S03Na NaO^S S03Na ch, ch, N h CH, :h, SO,Na CH=CH-|^^|= CH-CH^^N (ch2)4so3- (CH2)4S03Na SO,Na (ch2)4so3" (CH2)4S03Na SO,Na (ch2)4so3" (CH2)4S03Na & WO 00/16810 20 PCT/Li-99/07088 SO,Na (ch2)4so3- (CH2)4S03Na Na03S CH=CH I (ch2)4so3- C0NHCH2CH2S03Na S03Na CH-CH I (CH2)4S03Na (CH2)4S03 (CH2)4S03Na and y" ^ch^= ch-ch' (ch2)2so3- (CH2)2S03Na (25) The near infrared fluorescent contrast agent of (1) above, comprising at Wi jO/16810 21 PCT/EP99/07088 least one compound selected from the group consisting of the compounds of the following formulas CH3 CH=ecH-CH^N SO,Na (CH2)2S03~ (CH2)2S03Na NaOjS CH, CH3 CH3 ch3 I CH, CH=CH -C=CH-CH=CH-CH I CCH2)4SOj" SO,Na (CH2)4S03Na N (CH2)4S03- (CH2)4S03Na NaO^S SO,Na CH-Ctf^N I (CH2)2S03Na SO,Na (ch2)4so3" (CH2)4S03Na WO 00/16810 22 PCT/ iiP99/07088 NaO,S ch, CH, CH, CH, CH=CH)-CH^n I I SO-iNa (CH2)2S01 (CH2)2S03Na c2h5 Na03S(CH2)3 1^3 ch3 ch3 CH=^CH-CH%^n /C2H5 (ch2)3so3- (CH2)3S03Na (CH2)3S03Na NaO,S ch, ch3 ch3 ch=^= ch — ch^f^n SO,Na (CH2)3S03- (CH2)3S03Na Na03S(CH2>4 ch3 ch3 CH=^= CH-CH^f^N (CH2)4S03Na (CH2)4S03- (CH2)4S03Na -23- 10 NaO,S CH, CH3 CH=^= CH—CH^f^ N SO^Na (CH2)4S03- (CH2)4S03Na 15 NaO-iS CH, C2H5 C2H5 CH=f= CH — CH^ff^ N SO,Na (CH2)4S03~ (CH2)4S03Na 20 25 (CH2)4S03 (CH2)4S03Na 30 (CH2)4S03' (CH2)4S03Na wo 1)0/16810 pct/ep99/07088 24 CH3 (C2H5)3NH03 ch3 • CH3 W CH3- CHKTH-CHC-CHCH-CH^n S03HN(C2H5)3 I (CH2)4S03 I (CH2)4S03HN(C2H5)3 NaO-»S S03Na CH3 CH, CH, S03Na CH, n"^ch=f= ch—ch%^n SO,Na (CH2)3so3 (CH2)3S03Na (CH2)4so3- S03Na (CH2)4S03Na Na03SCH2CH202S ch, CH, CH-CH^n S02CH2CH2S03Na (CH2)2S03- (CH^SOjNa Ji > WC- J0/16810 25 pct/ep99/07088 CH, Na03SCH2CH2NH02 CH, CH, CH, CH=^= CH—CH%^N S02NHCH2CH2S03Na (CH^SOf (CH2)4S03Na NaOiS CH, ch, ch3 ch3 N^CH^CH—CH^n (CH2)3S03- (CH2)3S03Na SO,Na Na03SCH2CH2NHC< ch, CH3 CH3- :h, N CH=f CH—CH%^ N I C0NHCH2CH2S03Na (CH^SOf (CH2)4S03Na Na03SCH2CH2CH2' ch3 ch3 ch3 ch, J<^CH=^= CH —CH%^ N 0CH2CH2CH2S03Na (ch2)3so3 (CH2)3S03Na WO 00/16810 26 PCT/Li-99/07088 NaOoS SO,Na ch, CH, n^ch=^= ch-ch^n SOiNa ch2ch2chs07 ch3 ch2ch2chso 3Na ch, NaO,S ch, CH, n^^ch^ch—CH%^n SO-»Na (ch2)4s03- (CH2)4S03Na NaOOC ch, ch, ch, ch, n^ch=^= ch -ch^pn I (ch2)3s03 SO,Na (CH2)3S03Na ch, ch3 ch3 CH=^= CH-CH^n SO,Na (ch2)4so3- (CH2)4S03Na WO 00/16810 27 PCT/EP99/07088 (Na03SCH2CH2CH2)2N. CH, CH, N'^CH^ CH—CH%^N N(CH2CH2CH2S03Na)2 (CH^SOj (CH2)3S03Na NaOiS SO,Na (CH2)4S03 (CH2)4S03Na (CH2)4S03 (CH2)4S03Na (CH2)4S03" (CH2)4S03Na WO 00/16810 28 PCT/EP99/07088 (CH2)2CHS03 (CH2)2CH SO3N a I I ch3 ch3 (CH^CHSOj I ch3 I (CH2)2CHS03Na CH3 S03Na CH3 CH=CH I (CH^CHSC^ CH3 (CH2>2CHS03Na I CH3 Wv, 00/16810 29 PCT/EP99/07088 S0,Na (CHzkCHSCV CH3 (CH2)2CHS03Na I CH3 NaOjS CH, CH=CH ^3 CH^^n CH, CH, CH, SO,Na (CH2)2S03- (CH2)2S03Na NaOjS CH, CH, N*^CH=CH CH, (CH^SOj CH, CH-Ctf^N I (CH2)2S03Na S03Na S03Na WO 00/16810 30 PCT/tr99/07088 (CH2)4S0J (CH2)4S03Na (CH2)4S03- I (CHjljSOsNa Wv, 00/16810 31 PCT/EP99/07088 SO,Na (CH2)4S03~ (CH2)4S03Na NaO^S ch 3 SCH2CH2S03Na ch3 CH3 \ CH3- CH=CH (ch2)4so3~ SO,Na CH-CH<^n I (CH2)4S03Na S03Na (ch2)4s03- (CH2)4S03Na S03Na WO 00/16810 32 PCT/bf99/07088 NaOjS S03Na S03Na CH-CH^^n (ch2)4so3- (CH2)4S03Na NaOjS S03Na CH=CH I (CH2)4S03- ch-ch^^n S03Na I (CH2)4S03Na ,S03Na Wc d0/16810 PCT/EP99/07088 33 (ch2)4so3" SO,Na (CH2)4S03Na Na03S CH, CH, CH=CH (CH2)4S03" C0NHCH2CH2S03Na CH-CH^'N S03Na i (CH2>4S03Na ■ CH3 i~n3 ^n3- '5^ CH=CH-CH=C-CH=CH-CH I I (CH2>4S03" (CH2)4S03Na and WO 00/16810 34 PCT/EP99/07088 Na03S ch3 ch3 S03Na CH3 H3C N+^XH^=CH-CH N (ch2)2s03 (CH2)2S03Na 20 (26) The sodium salt of (14) above, wherein the monovalent group of A is substituted or unsubstituted alkyl, substituted or unsubstituted aryl, substituted or unsubstituted aralkyl, lower alkoxy, optionally substituted substituted amino, alkylcarbonyloxy, substituted or unsubstituted alkylthio, substituted or unsubstituted arylthio, cyano, nitro or halogen atom. brief description of the drawings Figs. 1 to 4 are photographs showing fluorescence imaging at 24 hours after administration of the compound, wherein administered were A.ICG (5 mg/kg), B:NK-1967 (5 mg/kg), C:compound (29) (0.5 mg/kg) and D:compound (6) K salt (5 mg/kg). Fig. 5 is a photograph showing fluorescence imaging at 24 hours after administration of the compound, wherein administered was E:compound (31) (5 mg/kg). Figs. 6 to 9 are photographs showing fluorescence imaging at 20 seconds and 5 minutes after administration of the compound (5 mg/kg), wherein administered were A:ICG (20 seconds later), B:ICG (5 minutes later), C.compound (29) (20 seconds later) and Drcompound (29) (5 minutes later). Fig. 10 is a graph showing the concentration of the compound in plasma at 0.5, 1,4 and 24 hours after administration of the compound, wherein the axis of ordinates is concentration (ng/ml) of the compound in plasma at each time point. 00/16810 -35- PCT/EP99/07088 Fig. 11 is a chart showing the infrared absorption spectrum of compound (29). Fig. 12 is a chart showing the infrared absorption spectrum of compound (31). 5 Fig. 13 is a chart showing the infrared absorption spectrum of compound (6). Fig. 14 is a chart showing the infrared absorption spectrum of compound (54). 10 DETAILED DESCRIPTION OF THE INVENTION The terms used in the present specification are defined in the following. The near infrared fluorescent contrast agent in the present invention means a is contrast agent that emits fluorescence in a near infrared region. In the present invention, the sulfonic acid group may mean sulfonate (-S03") when said sulfonic acid group is used to form an inner salt. In the present invention, preferable X and Y are of the following formula r3 I — c — :t4 wherein R3 and R4 are the same or different and each is substituted or unsubstituted alkyl. The alkyl of "substituted or unsubstituted alkyl" at R1, R2, R3 and R4 is preferably linear or branched lower alkyl having 1 to 5 carbon atoms, such as methyl, ethyl, 30 propyl, isopropyl, butyl, isobutyl, sec-butyl, tert-butyl, pentyl, isopentyl, neopentyl, tert-pentyl, 2-methylpropyl, 1,1-dimethylpropyl and the like. The substituent may be, for example, sulfonic acid group, carboxyl, hydroxy and the like. Examples of substituted alkyl include hydroxymethyl, 1-hydroxyethyl, 2- WO 00/16810 PCT/i£P99/07088 -36- 10 15 20 25 hydroxyethyh-Z-hydfOxypropyh-S-hydFOxypFopyM-hydr&xybuty^GaFboxymethy., carboxyethyl, carboxybutyl, sulfomethyl, 2-sulfoethyl, 3-sulfopropyl, 4-sulfobutyl and the like. Preferred R1 and R2 are lower alkyl having 1 to 5 carbon atoms that is substituted by sulfonic acid group (e.g., 2-sulfoethyl, 3-sulfopropyl, 4-sulfobutyl and the like), and R3and R4 are unsubstituted lower alkyl having 1 to 5 carbon atoms (e.g., methyl, ethyl and the like). The unsubstituted lower alkyl having 1 to 5 carbon atoms at R17 and R18 is exemplified by those mentioned above with regard to the alkyl of "substituted or unsubstituted alkyl" at R1, R2, R3 and R4. The alkyl group of the lower alkyl having 1 to 5 carbon atoms that is substituted by sulfonic acid group at R19 and R20is exemplified by those mentioned above with regard to the alkyl of "substituted or unsubstituted alkyl" at R1, R2, R3 and R4, and examples of the substituted lower alkyl having 1 to 5 carbon atoms include 2-sulfoethyl, 3-sulfopropyl and 4-sulfobutyl. The alkyl moiety of alkyl(sulfoalkyl)amino group, bis(sulfoalkyl)amino group, sulfoalkoxy group, (sulfoalkyl)sulfonyl group and (sulfoalkyl)aminosulfonyl group at R21 - R28 is preferably a linear or branched lower alkyl having 1 to 5 carbon atoms, which is exemplified by those mentioned above with regard to the alkyl of "substituted or unsubstituted alkyl" at R1, R2, R3 and R4. In the present invention, the "nonmetallic atoms necessary for forming a substituted or unsubstituted condensed benzo ring or condensed naphtho ring" means a bonding group necessary for forming a condensed benzo ring or condensed naphtho ring, which is a group of the formula Wo 00/16810 PCT/EP99/07088 -37- —Whenthe said bonding group may include a substituent. Specific examples thereof include carbon atom, nitrogen atom, oxygen atom, 5 hydrogen atom, sulfur atom, halogen atom (e.g., fluorine atom, chlorine atom, bromine atom and iodine atom) and the like. The substituent of the condensed benzo ring and condensed naphtho ring formed by the nonmetallic atoms at Z1 and Z2 is exemplified by sulfonic acid 10 group, carboxyl, hydroxy, halogen atom (e.g., fluorine atom, chlorine atom, bromine atom and iodine atom), cyano, substituted amino (e.g., dimethylamino, diethylamino, ethyl 4-sulfobutylamino, di-(3-sulfopropyl)amino and the like), and substituted or unsubstituted alkyl as defined above, which is bonded to the ring directly or via a divalent bonding group. Preferable divalent bonding group may 15 be, for example, -0-, -NHCO-, -NHSOr, -NHCOO-, -NHCONH-, -COO-, -CO-, SO2-, and the like. The alkyl of substituted or unsubstituted alkyl that is bonded to the ring directly or via a divalent connection group is exemplified preferably by methyl, ethyl, propyl and butyl, and the substituent is preferably exemplified by sulfonic add group, carboxyl and hydroxy. 20 The substituent of methine at L1 - L7 is exemplified by substituted or unsubstituted alkyl (defined above), halogen atom (defined above), substituted or unsubstituted aryl, lower alkoxy and the like. The aryl of "substituted or unsubstituted aryl" is exemplified by phenyl, naphthyl and the like, preferably 25 phenyl. Examples of the substituent include halogen atom (defined above, preferably chlorine atom) and the like. The substituted aryl indudes, for example, 4-chlorophenyl and the like. The lower alkoxy is preferably linear or branched alkoxy having 1 to 6 carbon atoms, which is specifically methoxy, ethoxy, propoxy, butoxy, tert-butoxy, pentyloxy and the like, preferably methoxy 30 and ethoxy. In addition, the substituents of methine at L1 - L7 may be bonded each other to form a ring containing three methine groups, and this ring may further form a condensed ring with a ring containing different methine group. The ring containing three methine groups that is formed by the bonding of the WO 00/16810 PCT/l£P99/07088 -38- substituentsofmethineatL1-rLLis^exemplified4Dy4,4-dimethylGyGlQhexeneFing and the like. The conjugated methine chain consisting of the groups of L1 - L7, and having a s ring is preferably the group of the formula (a): A wherein Z3 denotes nonmetallic atoms necessary to form a 5- or 6-membered ring and A is hydrogen atom or a monovalent group. The "nonmetallic atoms necessary to form a 5- or 6-membered ring" is 15 exemplified by those mentioned above. In the formula (a) and [111-2] to be mentioned later,.- or 6-membered ring at Z3 is exemplified by cyclopentene ring, cyclohexene ring, 4,4-dimethylcyclohexene ring and the like, with particular preference given to cyclopentene ring. 20 The monovalent group represented by A includes, for example, substituted or unsubstituted alkyl (defined above), substituted or unsubstituted aryl (defined above), substituted or unsubstituted aralkyl, lower alkoxy (defined above), substituted amino which is optionally substituted, alkylcarbonyloxy (e.g., 25 acetoxy), substituted or unsubstituted alkylthio, substituted or unsubstituted arylthio, cyano, nitro, halogen atom (defined above), and the like. As used herein, aralkyl of the "substituted or unsubstituted aralkyl" is exemplified by benzyl, 2-phenylethyl, 1-phenylethyl, 3-phenylpropyl and the like, and the substituent may be sulfonic acid group, carboxyl, hydroxy, substituted or 30 unsubstituted alkyl (defined above), alkoxy (defined above), halogen atom (defined above), and the like. The substituted amino of the "substituted amino which is optionally substituted" includes, for example, alkylamino (e.g., methylamino, ethylamino and the like), dialkylamino (dimethylamino, 10 == CH — CH WO 00/16810 PCT/EP99/07088 -39- 10 15 (e.g., morpholino, imidazolidino, ethoxycarbonylpiperadino and the like) and the like. The substituent with regard to the optional substitution of the "substituted amino which is optionally substituted" includes sulfonic acid group, carboxyl and the like. The alkylthio of the "substituted or unsubstituted alkylthio" may be, for example, methylthio, ethylthio and the like. Examples of the substituent include sulfonic acid group, carboxyl and the like. The arylthio of the "substituted or unsubstituted arylthio" is exemplified by phenylthio, naphthylthio and the like. Examples of the substituent include sulfonic acid group, carboxyl and the like. The monovalent group represented by A is preferably fluorine atom, chlorine atom, dialkylamino (preferably having 6 or less carbon atoms, and optionally forming a ring) or morpholino. This group particularly preferably has a sulfonic acid group. In the formula [I], r is preferably 1. The pharmaceutically acceptable salt may be any as long as it forms a nontoxic salt with the compound of the formula [I]. Examples thereof include alkali metal salts such as sodium salt, potassium salt; salt of alkaline earth metal such as 20 magnesium salt, calcium salt and the like; organic ammonium salt such as ammonium salt, triethyl ammonium salt, tributyl ammonium salt, pyridinium salt and the like; salt of amino acid such as lysine salt, arginine salt and the like. Particularly preferred is sodium salt causing less toxicity in the living body. 25 The fluorescent contrast agent to be used in a living body should be particularly water soluble. In the present invention, the near infrared fluorescent contrast agent has a noticeably improved water solubility by the introduction of 3 or more sulfonic acid groups into the above-mentioned compound. For superior water solubility, the number of the sulfonic acid groups is preferably 4 or more. For 30 easy synthesis, the number of the sulfonic acid groups is not more than 10, preferably not more than 8. The improvement in the water solubility can be determined by measuring partition coefficient of each compound, which for example, may be measured in a two-phase system of butanol/water. More WO 00/16810 -40- PCT/EP99/07088 speeifieallyHntroduetion of 3 eFmoFe-sulfenie^eid-greops-Fesolts-in-a partitie coefficient log Po/w of n-butanol/water of not more than -1.00. The sulfonic acid groups are particularly preferably introduced into the positions 5 of R1, R2, Z1 and/or Z2 of the formula [I] and R1, R2, R5, R7, R11 and/or R13 of the formula [II]. In addition, these sulfonic acid groups are preferably introduced into L4 of the conjugated methine chain at the position A of the above-mentioned formula (a) 10 via a divalent group such as alkylene. Of the sodium salts of compounds of the formula [II] having three or more sulfonic acid groups in a molecule, preferred is a sodium salt of a compound wherein R1 and R2 are lower alkyl having 1 to 5 carbon atoms which is is substituted by sulfonic acid group, and X and Y are the same or different and R17 I I R18 each is a group of the formula wherein R17 and R18are the same or different and each is unsubstituted lower alkyl having 1 to 5 carbon atoms alkyl, said salt having three or more sulfonic acid groups in a molecule, with particular preference given to a compound of the 20 formula (CH2)4S03~ (CH2)4S03Na WO 00/16810 PCT/EP99/07088 -41- ©fihexompountisrofthe formula [I] having three or more sultonic acid groups in a molecule and pharmaceutically acceptable salts thereof, preferred is a sodium salt of a compound of the formula [111-1] 10 [Ill-l] wherein L1 - L7 are as defined above, R19 and R20 are lower alkyl having 1 to 5 carbon atoms which is substituted by sulfonic acid group, and R21 to R28 are the same or different and each is hydrogen atom, sulfonic acid group, carboxyl group, hydroxyl group, alkyl(sulfoalkyl)amino group, bis(sulfoalkyl)amino group, sulfoalkoxy group, (suifoalkyl)sulfonyl group or (sulfoalkyl)amino-sulfonyl group, and X' and Y' are the same or different and each is a group of the formula .17 — C — I R 18 wherein R17 and R18are as defined above, said salt having three or more sulfonic acid groups in a molecule, with particular preference given to the compound of the following formula. 15 i (CHzfcSOj" (CH2)2S03Na 20 WO 00/16810 PCT/El'99/07088 -42- 10 IS 20 25 ©fthesodium saits^ofthe^3ompoundsoftheformula[l 11-1 Jhavingthree or more sulfonic acid groups in a molecule, preferred is a sodium salt of a compound of the formula [III-2] wherein R19 - RZB, X' and Y* are as defined above, Z3 is nonmetallic atoms necessary to form a 5- or 6-membered ring and A is hydrogen atom or a monovalent group, said salt having three or more sulfonic acid groups in a molecule, with particular preference given to the compound of the following formula >28 [iii-2] NaO,S S03Na (CH2)4S03~ (CH2)4S03Na The compounds contained in the inventive near infrared fluorescent contrast agent may be any as long as it has formula [I] or [II], as well as 3 or more, preferably 4 or more, sulfonic acid groups in a molecule. These compounds can be synthesized according to a known production method of cyanine dye compounds disclosed in The Cyanine Dyes and Related Compounds, F.M. Hamer, John Wiley and Sons, New York, 1964 , Cytometry, 10,3-10 (1989), Cytometry, 11,418-430 (1990), Cytometry, 12 723-730 (1990), Bioconjugate Chem. 4,105-111 (1993), Anal. Biochem., 217,197-204 (1994), Tetrahedron, 45,4845-4866 (1989), EP-A-0591820A1, EP-A-0580145A1, and the like. Alternatively, they can be semisynthesized from a commercially available cyanine dye compound by a known method. To be specific, they can be synthesized by reacting a dianyl compound and a heterocyclic quaternary salt. WO 00/16810 -43- PCT/EP99/07088 The compound of the formula [I] of the present invention can be synthesized by, for example, the following method. (i)when r=0 5 (aJL^L5, X=Y, R1=R2 and Z1=Z2 A hetero ring quaternary salt compound (2 moles) of the formula [IV-1] l1r2 pv-1] R1 wherein L1, X, Z1 and R1 are as defined above, and a dianyl compound (1 mole) of the formula [V-1] 15 NH-L2=L3-L4=N—i \ ' HC1 [V-1] wherein L2, L3 and L4 are as defined above, are reacted in the presence of a 20 base and a solvent to give a compound of the formula [VI-1] Z1 [VI-1] wherein L1, L2, L3, L4, R1, Z1 and X are as defined above, and this compound [VI-1] (1 mole) and a necessary molar amount of the compound of the formula [VII] 30 T1 - Na [VII] wherein T1 is an organic acid residue, are reacted to give a sodium salt of the compound of the above-mentioned formula [VI-1]. (b) LVL5 or X*Y or RVR2 or Z1#Z2 25 R1 WO 00/16810 -44- PCT/EP99/07088 15 A-hetero-ring-quatemary^salbcompoanch(Jhmole)-ofihe-above= 7 mentioned formula [IV-1] and the above-mentioned dianyl compound (1 mole) of the formula [V-1] are reacted in the presence of a base and a solvent to give a compound of the formula [VIII-1] •X ^—LI=L2-L3=L4—N—/ \ • HC1 [Vm-1] 10 wherein L1, L2, L3, L4, R1, Z1 and X are as defined above, and this compound [VIII-1] (1 mole) and a hetero ring quaternary salt compound (1 mole) of the formula [XI-1] [XI-1] wherein L5, Y, Z2 and R2 are as defined above, are reacted to give a compound of the formula [X-1] 20 25 30 — LI=L2-L3==L4-L5==^ R1 R [X-1] wherein L1, L2, L3, L4, L5, R1, R2, Z1, Z2, X and Y are as defined above, and this compound of the formula [X-1] (1 mole) and a necessary molar amount of the above-mentioned compound of the formula [VII] are reacted to give a sodium salt of the compound of the above-mentioned formula [X-1]. (ii) when r=1 (a) L1=L7, X=Y, R1=R2 and Z1=Z2 A hetero ring quaternary salt compound (2 moles) of the formula [IV-1] WO 00/16810 PCT/EP99/07088 -45- 10 15 20 LH- [IV-1] wherein L1, X, Z1 and R1 are as defined above, and a dianyl compound (1 mole) of the formula [V-2] ^ ^— NH-L2=L3-L4=L5-L6=N —^ • HCl [V-2] wherein L2, L3, L4, L5and L® are as defined above, are reacted in the presence of a base and a solvent to give a compound of the formula [VI-2] L1=L2-L3=L4-L5=L6-L1 | Z1 [VI-2] wherein L1, L2, L3, L4, L5, L6, R1, Z1 and X are as defined above, and this compound [VI-2] (1 mole) and a necessary molar amount of the compound of the formula [VII] T1 -Na [VII] 25 wherein T1 is as defined above, are reacted to give a sodium salt of the compound of the above-mentioned formula [VI-2]. (b) L W or X*Y or RVR2 or ZVZ2 A hetero ring quaternary salt compound (1 mole) of the above-mentioned formula [IV-1] and the above-mentioned dianyl compound (1 mole) of 30 the formula [V-2] are reacted in the presence of a base and a solvent to give a compound of the formula [VIII-2] WO 00/16810 -46- PCT/EP99/07088 10 l1=l2-l3=l4-l5=l6- /"A. HCl [vni-2] R1 wherein L1, L2, L3, L4, L5, L6, R1, Z1 and X are as defined above, and this compound [VIII-2] (1 mole) and a hetero ring quaternary salt compound (1 mole) of the formula [IX-2] [IX-2] 15 wherein L7, Y, Z2 and R2 are as defined above, are reacted to give a compound of the formula [X-2] 20 25 30 L'=L2-L3=L4-L5=L6-L7==/ I [x-2] N" I R1 R' wherein L1, L2, L3, L4, L5, L8, L7, R1, R2, Z1, Z2, X and Y are as defined above, and this compound of the formula [X-2] (1 mole) and a necessary molar amount of the above-mentioned compound of the formula [VII] are reacted to give a sodium salt of the compound of the above-mentioned formula [X-2]. (iii) when r=2 When r is 2, L6 and L7 overlap in the formula [I]. To avoid this, the overlapping L6 and L7are referred to as L8 and L9 for clarification. (a) L1=L9, X=Y, R1=R2 and Z1=Z2 WO 00/16810 PCT/EP99/07088 _47. "A hetercrring quaternary salt compound (2 moles) ofthe formula 10 [IV-1] [iv-1] wherein L1, X, Z1 and R1 are as defined above, and a dianyl compound (1 mole) of the formula [V-3] /~\ nh-l2=l3-l4=l5-l6=l7-l8=n- " ^ HCl [v-3] wherein L2, L3, L4, L5, L6and L7are as defined above and L8 is an optionally is substituted methine group, are reacted in the presence of a base and a solvent to give a compound ofthe formula [VI-3] 20 l1=l2-l3=l4-l5=l6-l7=l8-l1 l [VI-3] wherein L\ L2, L3, L4, L5, L6, L7, L8, R1, Z1 and X are as defined above, and this compound [VI-3] (1 mole) and a necessary molar amount of the compound of 2s the formula [VII] T1 - Na [VII] wherein T1 is as defined above, are reacted to give a sodium salt of the compound of the above-mentioned formula [VI-3]. 30 (b) LVL9 or X*Y or RVR2 or ZVZ2 A hetero ring quaternary salt compound (1 mole) of the above-mentioned formula [IV-1] and the above-mentioned dianyl compound (1 mole) of the formula [V-3] are reacted in the presence of a base and a solvent to give a WO 00/16810 PCT/EP99/07088 -48- GGmpound^oMhefoFmula-p/IH-3]' L1 =L2-L3=L4 -L5=L6-L7=L8" wherein L1, L2, L3, L4, L5, L6, L7, L8, R1, Z1 and X are as defined above, and this compound [VIII-3] (1 mole) and a hetero ring quaternary salt compound (1 mole) 10 of the formula [IX-3] -Y 15 [IX-3] 20 wherein Y, Z2and R2are as defined above and L9is an optionally substituted methine group, are reacted to give a compound of the formula [X-3] L1=L2-L3=L4-L5=L6-L7=L8-L9=^ [X-3] 25 wherein L1, L2, L3, L4, L5, L6, L7, L8, L9, R1, R2, Z1, Z2X and Y are as defined above, and this compound ofthe formula [X-3] (1 mole) and a necessary molar amount of the above-mentioned compound ofthe formula [VII] are reacted to give a sodium salt of the compound of the above-mentioned formula [X-3]. 30 The necessary molar amount of the compound of the formula [VII] is not less than the amount equivalent to the amount of sodium contained in one molecule of the objective sodium salt of the compound of the formula [I]. -49- ^e-stit»stitcrentx>fthe substituted methine group at L8 and L3 Is exemplified by those mentioned with regard to the substituent ofthe above-mentioned methine groups at L1 to L7. 5 In the synthetic methods of the above-mentioned (i), (ii) and (iii), the reaction of the compounds [IV-1] and [V-1], that ofthe compounds [VIII-1] and [XI-1], that of the compounds [IV-1] and [V-2], that of the compounds [VIII-2] and [IX-2], that of the compounds [IV-1] and [V-3] and that ofthe compounds [VIII-3] and [IX-3] proceed at a temperature of -20°C - 80°C, preferably -10°C - 40°C, preferably 10 in the presence of an acylating agent such as acetic anhydride. In the synthetic methods ofthe above-mentioned (i), (ii) and (iii), the reaction of the compounds [IV-1] and [VII], that ofthe compounds [X-1] and [VII], that ofthe compounds [VI-2] and [VII], that ofthe compounds [X-2] and [VII], that ofthe 15 compounds [VI-3] and [VII] and that of the compounds [X-3] and [VII] proceed at a temperature of preferably 0°C - 40°C, preferably in the presence of a solvent such as alcohol and water. In the synthetic methods of the above-mentioned (i), (ii) and (iii), the base to be used may be, for example, triethylamine, tributylamine, pyridine, diazabicycloundecene, sodium methoxide and the like; the solvent to be used may be, for example, an amide compound such as N,N-dimethylacetamide, N-methylpyrrolidone and N,N-diethylformamide or alcohols such as methanol; and the organic acid residue may be, for example, CH3COO and the like. With regard to the production of various pharmaceutically acceptable salts of the compounds of the aforementioned formula [I], ammonium salt and potassium salt of the compounds of the formula [I] can be obtained by, for example, substituting the compound of the formula [VII] used in the above-mentioned 30 synthetic methods (i), (ii) and (iii) with a compound of the formula [VII] wherein the sodium atom has been changed to ammonium group or potassium atom; and different cationic salts of the compounds of the aforementioned formula [I] can be obtained by converting said ammonium salt and potassium salt to WO 00/16810 PCT/EP99/07088 -50- differentcationic salts by theuse of ion exchange resins as necessary. The compound of the above-mentioned formula [I] inclusive of the compound of the formula [II] to be used in the present invention are specifically exemplified in 5 the following, to which the present invention is not limited. (1) (2) ch3 ch3 -ch3 ch3- I^CH=f CH-CH^n I (ch^soj S03Na Na03S CH3 " a (CH2)2S03Na ch3 ■ch3 ch3 CHt ot<^-c=ct-ch=ch-c}4 ^ XT SC^Na I (CH2)4SCJJ I (CH2)4S03Na (3) Na03S\^^ CH3 n ch3 -ch3 ci ch3-I ch=ch-ch=ch-c=ch-ch^ n XX SC^Na I (CH^SOJ I (CH2)4S03Na (4) ch3 -ch3 N^^CH=€H I (CH^SC^ ch3- Zl3 her SO^Na CH-CHrV I (CHzhSOaNa (5) ch3 a ■ch3 ch3 ch3 ch3-I 5^ ch=ch-ch=c-ch=ch-ch ^ n xx SQjNa (CH^SOf (CH2)4S03Na *0 t, "» . Wo 00/16810 PCT/EP99/07088 -51- (6) (7) SOiNa NaO,S (CH^SOj CH, CH, CH=CH ^-CH^^n I I (CH^SOj (CH^SOjNa (8) C2H5 Na03S(CH2)3 CH, CH, ch-CH^n I I (CH2)3S03 (CH^SOjNa /C2H5 (CH2)3S03Na (9) NaO,S CH, CH, CH3 CH3 CH=£ CH — CH N (CH^SOj (CH2)3S03Na S03Na (10) Na03S(CH2)4 CH, CH3- CH-CH^f^N I I (CH2)4S03Na (CH2)4S03 (CH2)4S03Na 52 (11) Na03S (12) Na03S n ch3 ch3 ch: CH3 ch, :CH— CH^TJ1 S03Na (ch2)4s03- (CH2)4S03Na V N ch3 c2h5 ch=€ch— cht3 (ch2)4so3- ch3 c2h5 S03Na (CH2)4S03Na NaOjS. NaOjS-CHjCHj. S03Na ch=ch-ch=c-ch=ch-ch I (ch2)4s03 SQ3Na (15) (CH2)4so3 (CH2)4S03HN (C2H5)3 Wu 00/16810 PCT/EP99/07088 xi&r -53- Na03S CH, SO,Na CH, CH _ch^Vn SO,Na (CH2)3S03 (CH2)3S03Na (17) S03Na CH3 SO,Na CH, n^^ch=^= ch ~ch%^n Na03S (CH2)4S03 | S03Na (CH2)4S03Na (18) Na03sch2ch202S CH—CH^n I I S02CH2CH2S03Na (CH2)2S03 (CH2)2S03Na (19) ch, Na03SCH2CH2hffl02SY^%r ■ch3 ch3- -^N^CH^CH—CH^N I I XJ S02NHCH2CH2S03Na (ch^soj (CH2)4S03Na WO 00/16810 -54- PCT/LF99/07088 (21) Na03SCH2CH2NHC Xt ch3 ch3 ch3 CH, n I (ch2)4so3 ch=€ch— cl&z n xr C0NHCH2CH2S03Na (CH2)4S03Na (22) Na03SCH2CH2CH2< XT ch3 ch3 CH, CH, N I (CH2)3so3" ch=€ch—ci^n XT 0CH2CH2CH2S03Na (CH2)3S03Na (23) Na03S S03Na ch3 Na03S ch| ch, S03Na ch2ch2chso3 ch3 cH^fy CH2CH2CHS03Na CH3 (24) Na03S S03Na (CH2)4S03 (CH2)4S03Na *0 WO 00/16810 PCT/EP99/07088 -55- (25) NaOOC CH CH, ?3 CH=^=CH—CH^T^n SOjNa (CH^SOj" (CH2)3S03Na (26) (CH2)4S03" (CH2)4S03Na (28) SO,Na -56- (29) NaO,S (30) SOjNa CH, CH, CH, CH=£= CH — CH^T\ , N '3 N SOjNa CH2)4so3 CH2)4S03Na (32) Na03S SOjNa WO 00/16810 57 PCT/EP99/07088 (33) NaC^S (34) ch=ch ^ n S03Na (ch2)2chs03 I ch3 (CH2)2CH S03N a I ch3 (35) (36) NaO,S (CH^CHSOj I ch3 I (CH2)2CHS03Na CH3 (CH^CHSOj ch3 (CH2)2CHS03Na I ch3 CH=CH I (ch2)2chs03~ ch3 ch, ch, CH-CH^^N I SO,Na (CH2)2CHS03Na I ch3 WO 00/16810 58 PCT/El'99/07088 (37) Na03S CH, CH, CH, ch=CH5t CH^n S03Na (CH2)2S03* (CH2)2S03Na (38) NaQ3S (39) CH, CH, CH=CH CH, (CH^SOj CH, CH-CH^^N (CH2)2S03Na SO,Ma (40) ■) Wv>aM)/16810 59 PCT/EP99/07088 (41) (42) (43) NaOjS SOiNa CH2CH2S03Na CH3 CH=CH-CH=C-CH=CH-CH I (CH2)4so3- SO,Na (CH2)4S03Na (44) (CH2)4S03~ (CH2)4S03Na WO 00/16810 60 PCT/El'99/07088 (45) NaOjS ch 1 SCH2CH2S03Na CH3 \ CH3- CH=CH :h. SOiNa CH-CH^N (CH2)4S03- I (CH2)4S03Na (46) CH3 CH=CH-/S= ( (CH2)4so3- SOiNa (CH2)4S03Na (47) (48) (CH2)4S03- (CH2)4S03Na WO 00/16810 61 PCT/EP99/07088 "N ^ CH=CH (CH2)4S03 SO,Na (50) NaO,S (CH2)4S03Na H CH2CH2S03Na \7 CH3 CH=CH-|^N=CH-CH^N SO,Na (CH2)4S03~ SOiNa (CH2)4S03Na (51) Na03S S03Na CH3 SQ3Na CH-CH^^N I (CH2)4S03Na (52) Na03S CH, NC CH, 5^ CH=CH / 'CH C0NHCH2CH2S03Na SO,Na CH-CH (CH2)4S03- I (CH2)4S03Na WO 00/16810 62 PCT/Lr99/07088 (53) CH3 SO,Na NaO,; ch3 ch3 ch3 1 ch=ch-ch=c-ch=ch-ch n (CH2)4S03- (CH2)4S03Na . (54) Na03S CH3 ch3 S03Na CH3 H3C N+^CH^=CH-CH^^ N (CH2)2S03 (CH2)2S03Na The above-mentioned compound to be contained in the near infrared fluorescent contrast agent of the present invention shows absorbance and fluorescence in the near infrared light region of 700 -1300 nm, particularly about 700 - 900 nm, and has a molar absorption coefficient of not less than The near infrared fluorescent contrast agent of the present invention is subject to no particular limitation as long as it contains a compound of the formula [I] or formula [II] and/or a pharmaceutically acceptable salt thereof, and has 3 or more, preferably 4 or more, sulfonic acid groups in a molecule. This compound or a salt thereof alone or in combination may be contained in said contrast agent. To be specific, said contrast agent includes said compound or said compound suspended or dissolved in a solvent, such as injectable distilled water, physiological saline, Ringer solution and the like. Where necessary, pharmacologically acceptable additives such as carrier, excipient and the like 100,000. Wv, 00/16810 63 PCT/EP99/07088 may be added. These additives contain substances such as pharmacologically acceptable electrolyte, buffer, detergent and a substance for adjusting osmotic pressure and improving stability and solubility (e.g., cyclodextrin, liposome and the like). Various additives generally used in the pertinent fields may be used. The near infrared fluorescent contrast agent of the present invention is preferably produced through a sterilization process when it is intended for pharmaceutical use. Said contrast agent can be administered to a living body by injecting, spraying or coating, intravascular^ (venous, arterial), orally, intraperitoneally, percutaneously, subcutaneously, intracystically or intrabronchially. Preferably, the agent is administered into blood vessels in the form of an aqueous agent, emulsion or suspension. The dose of the near infrared fluorescent contrast agent of the present invention is not particularly limited insofar as the dose enables detection of the site to be ultimately diagnosed, it is appropriately adjusted depending on the kind of compound to be used that emits near infrared fluorescence, age, body weight and target organ of administration subjects, and the like. Typically, the dose is 0.1 -100 mg/kg body weight, preferably 0.5 - 20 mg/kg body weight, in the amount of said compound. The contrast agent of the present invention can be appropriately used for various animals other than human. The administration form, route and dose are suitably determined depending on the body weight and conditions ofthe target animals. In the present invention, moreover, the above-mentioned compound of the formula [I], particularly preferably [II], having 3 or more, preferably 4 or more, sulfonic acid groups in a molecule tends to be noticeably accumulated in tumor tissues. Utilizing this characteristic, a tumor tissue can be specifically imaged using the inventive fluorescent contrast agent. In addition, a series of said WO 00/16810 64 PCT/EM9/07088 compounds can reside in blood vessel for a long time and they are expected to serve well as angiography contrast agents. The fluorescence imaging method of the present invention is characterized by the use of the inventive near infrared fluorescent contrast agent. This method is practiced following known methods, and each parameter, such as excitation wavelength and fluorescence wavelength to be detected, is appropriately determined to achieve optimal imaging and evaluation, depending on the kind of near infrared fluorescent contrast agent to be administered and administration targets. The time spent from administration of the inventive near infrared fluorescent contrast agent to determination target to the initiation of determination by the inventive fluorescence imaging method varies depending on the kind of the near infrared fluorescent contrast agent to be used and administration targets. For example, when the agent contains a compound of the formula [I] for tumor imaging, the lapse time will be about 4-120 hours after administration. In the case of compound of formula [II], the lapse time will be about 24-120 hours after administration. When the lapse time is too short, the fluorescence is so intense that the target site and other site cannot be clearly divided. When it is too long, said contrast agent may be cleared from the body. When imaging of blood vessel is desired, the compound of the formula [I] or formula [II] is detected immediately after administration or in about 30 minutes thereafter. The method typically includes the following steps. That is, a near infrared fluorescent contrast agent of the present invention is administered to a detection target and the detection target is exposed to an excitation light from an excitation light source. Then, fluorescence from the near infrared fluorescent contrast agent, which has been caused by said excitation light, is detected with a fluorescence detector. The wavelength for excitation varies depending on the near infrared fluorescent contrast agent to be used. It is free of limitation as long as said YvO 00/16810 65 PCT/EP99/07088 compound efficiently emits fluorescence in the near infrared region. Preferably, a near infrared light having superior biotransmission capability is used. The wavelength of the near infrared fluorescence to be detected also varies depending on the contrast agent to be used. In general terms, an excitation light having a wavelength of 600-1000 nm, preferably 700-850 nm, is used and near infrared fluorescence in a region at a wavelength of 700-1000 nm, preferably 750-900 nm, is detected, in this case, the excitation light source may be a conventional excitation light source, such as various lasers (e.g., ion laser, dye laser and semiconductor laser), halogen light source, xenon light source and the like. Where necessary, various optical filters may be used to obtain optimal excitation wavelength. Likewise, fluorescence may be detected using various optical filters to pick up only fluorescence from said near infrared fluorescent contrast agent. The detected fluorescence is data-processed as fluorescence information and used to generate fluorescence images that can be recorded. The fluorescence images are generated by irradiating a wide area including the target tissue, detecting fluorescence with a CCD camera and image-processing the obtained fluorescence information. Alternatively, an optical CT device may be used, an endoscope may be used, or a fundus camera may be used. The fluorescence imaging method of the present invention enables visualizing systemic diseases, tumors, blood vessels and the like without damaging a living body. The present invention is explained in more detail by way of Examples and Experimental Examples, to which the present invention is not limited. The compound numbers in the following Examples and Experimental Examples correspond to those of the compounds explained by structural formulas. WO 00/16810 PCmr"99/07088 The compound wherein a symbol designating "potassium salt", "calcium salt" or "pyridinium salt" is indicated after the compound number (e.g. compound (29) K salt) means a compound that is the same as the compound expressed by the compound number (sodium salt) except that the counter ion is potassium salt, calcium salt or pyridinium salt instead of sodium salt. For example, "compound (31 )K salt" means a compound that is the same as the compound (31) except that the counter ion is potassium instead of sodium; "compound (31)Ca salt" means a compound that is the same as compound i (31) except that the counter ion is calcium instead of sodium; and "compound (31)pyridiniurTr salt means a compound that is the same as compound (31) except that the counter ion is pyridinium instead of sodium. the synthetic method ofthe compound to be contained in the near infrared fluorescent contrast agent of the present invention as an active ingredient is explained in Examples. The following synthetic methods mostly consist of reactions of heterocyclic quaternary salt compound shown in Table 1 and dianyl compounds shown in Tables 2 and 3. Vv*/ 00/16810 67 PCT/EP99/07088 Table 1 Heterocyclic quaternary salt compound Symbol Structural formula [Registration No. in Chemical Abstract (CA)] Source/Synthetic method Q1 HO,S S03H In the same manner as Q2, corresponding indolenin derivative is reacted with butanesulton. NT "CH3 I 3 (ch2)4so3- (138913-76-5) Q2 H03S JP-A 63-55544 EP 251282 (113995-56-5) CH2CH2CHS03 CHa Q3 HO,S JP-A 2-233658 CA 114:122053 (76588-81-3) N+' un3 I 3 (CH2)4S03- WO 00/16810 68 PCT/L* y9/07088 HO3S, CH, ■CH, Q4 (183272-36-8) N"" "CH3 CH2CH2S03" In the same manner as Q3, corresponding indoienin derivative is reacted with 2-bromoethanesulfonic acid. Wo 00/16810 69 PCT/EP99/07088 Table 2 Dianyl compound-1 Symbol Structural formula [Registration No. in Chemical Abstract (CA)] Source/Synthetic method A1 ^ ^ NHCH=CH-CH=CH-CH=N ^ ^ (1497-49-0) *HCI Reagent commercially available from Aldrich and others A2 /~V_ iH3 r V NHCH=CH-C=CH-CH=N P V (1979-58-4) * HCl JP-A 8-295658 CA 126:90721 A3 ^ ^ NHCH=^^ CH=N—^ ^ •HCl (53019-66-2) Zh.Org.Khim., 13(6) 1189-92 (1977) CA 87:102034 A4 CI ^ ^ NHCH=^^—CH=N—^ ^ •HCl (63856-99-5) Zh.Org.Khim., 13(6) 1189-92 (1977) CA 87:102034 WO 00/16810 7n PCT/EP99/07088 Table 3 Dianyl compound-2 Symbol Structural formula [Registration No. in Chemical Abstract (CA)] Source/Synthetic method A5 f~\- ^ ^ y NHCH=C-CH=CH-CH=N—V J (77146-76-0) • HCl Nukleofir nye Reacts. Karbonil' nykn Soedin (1982), 52-53 CA 101:130179 A6 1H2CH2S03H V V-NHCH=CH-C=CH-CH=N—V ^ (125577-71-1) •HCl Ger Offem, DE 2928184 CA 94:176696 A7 ^ ^ NHCH=^^^-CH=N ^ ^ •HCl (56709-94-5) Zh.Org.Khim., 18(10) 2176-9 (1982) CA 98:73808 A8 CI ^ NHCH=j^^pCH=N--^ ^ (63857-00-1) *HCI Zh.Org.Khim., 13(6) 1189-92 (1977) CA 87:102034 -71-Examples- In the following Examples, the compounds are referred to with the symbols (e.g., A1, Q1 and the like) used in Tables 1 to 3 for the convenience's sake. 5 Example 1 : Synthesis of compound (29) To heterocyclic quaternary salt compound Q1 (5 g) were added methanol (100 ml), N,N-dimethylformamide (25 ml), triethylamine (5.6 ml), dianyl compound A1 (1.83 g) and acetic anhydride (3 ml), and the mixture was stirred at room 10 temperature for 4 hours. Triethyl amine (2.2 ml) and acetic anhydride (2 ml) were added, and the mixture was stirred at room temperature for 3 hours. The insoluble matter was filtered off, and a solution of sodium acetate (2 g) in methanol (15 ml) was added to the filtrate, which was followed by stirring at room temperature for 1 hour. The resulting crystals were collected by filtration is and washed with a small amount of methanol. To the obtained crude crystals (3.5 g) was added water (20 ml) for dissolution. Sodium acetate (1 g) was added, and then methanol (30 ml) was added, which was followed by stirring for 1 hour. The resulting crystals were collected by filtration, washed with a small amount of methanol and dried to give 3 g of compound (29). The obtained 20 compound (29) showed yellow in a flame test. Maximum wavelength of absorbance (H20): 780 nm .Molar absorption coefficient (H2O): 243,000 Maximum wavelength of fluorescence emission (H20): 802 nm The infrared absorption spectrum was measured for the obtained 25 compound (29) by potassium bromide tablet method using a Fourier transform infrared spectrometer (VALOR-III, manufactured by JASCO). The following peaks were detected. The spectrum is shown in Fig. 11. IR (<max(KBr)): 1414,1086,1037, 995, 889 cm"1 30 Example 2 : Synthesis of compound (34) To heterocyclic quaternary salt compound Q2 (2.13 g) was added methanol (20 ml) and the mixture was cooled to 10°C. Thereto were added dianyl 10 -72- -compoundA2(Ch75-g)r mixture was stirred for 20 minutes. Acetic anhydride (2 ml) was added, and the mixture was stirred at 10 °C for 4 hours. The insoluble matter was filtered off, and a solution of sodium acetate (2 g) in a small amount of methanol was added to the filtrate. The resulting crystals were collected by filtration and washed with a small amount of methanol. To the obtained crude crystals was added water (7ml) for dissolution. Methanol (7 ml) was added to precipitate crystals. The resulting crystals were collected by filtration, washed with a small amount of methanol and dried to give 1.2 g of compound (34). The obtained compound (34) showed yellow in a flame test. .Maximum wavelength of absorbance (H20): 794 nm .Molar absorption coefficient (H20): 176,000 .Maximum wavelength of fluorescence emission (H20): 812 nm 15 Example 3 : Synthesis of compound (6) To heterocyclic quaternary salt compound Q3 (9.5 g) are added methanol (50 ml), triethylamine (7 ml), dianyl compound A3 (3.1 g) and acetic anhydride (3.9 ml), and the mixture is stirred at room temperature for 7 hours. The insoluble matter is filtered off, and a solution of sodium acetate (5 g) in a small amount of 20 methanol is added to the filtrate. The mixture is stood overnight. The resulting crystals are collected by filtration and washed with a small amount of methanol. To the crystals is added water (30 ml) for dissolution. Sodium acetate (2 g) is added, and then methanol (30 ml) is added. The resulting crystals are collected by filtration, washed with a small amount of methanol and dried to give 25 compound (6). Example 4 : Synthesis of compound (45) To heterocyclic quaternary salt compound Q3 (4.8 g) were added methanol (50 ml), triethylamine (4 ml), dianyl compound A4 (1.7 g) and acetic anhydride (2 30 ml), and the mixture was stirred at room temperature for 3 hours. The insoluble matter was filtered off, and a solution of sodium acetate (4 g) in a small amount of methanol was added to the filtrate. The resulting crystals WO 00/16810 73 PCT/EP99/07088 were collected by filtration and washed with a small amount of methanol. To the crystals was added water (10 ml) for dissolution. Then methanol (10 ml) was added. The resulting crystals were collected by filtration, washed with a small amount of methanol and air dried to give 1.6 g of a compound that is the same as compound (45) except that the substituent on the methine chain is -CI instead of -SCH2CH2S03 Na. The above step was repeated to give 4.2 g of said compound. Thereto were added water (30 ml), triethylamine (1.2 ml) and sodium 2-mercaptoethanesulfonate (0.8 g), and the mixture was stirred at room temperature for 4 hours. The insoluble matter was filtered off, and a solution of sodium acetate (2 g) in a small amount of water was added to the filtrate. The resulting crystals were collected by filtration, washed with methanol (20 ml) and air dried to give 2.3 g of compound (45). The obtained compound (45) showed yellow in a flame test. Maximum wavelength of absorbance (H20): 815 nm Molar absorption coefficient (H20): 196,000 Maximum wavelength of fluorescence emission (H2O): 827 nm Example 5: Synthesis of compound (2) To heterocyclic quaternary salt compound Q3 (4.7 g) are added methanol (25 ml), triethylamine (2.8 ml), dianyl compound A5 (1.5 g) and acetic anhydride (2.4 ml), and the mixture is stirred at room temperature for 1 hour. Thereto are further added triethyl amine (3.5 ml) and acetic anhydride (1.5 ml), and the mixture is stirred at room temperature for 3.5 hours. The insoluble matter is filtered off, and a solution of sodium acetate (3 g) in a small amount of methanol is added to the filtrate. The mixture is stirred at room temperature for 1 hour. The resulting crystals are collected by filtration and washed with a small amount of methanol. To the crystals is added water (15 ml) for dissolution. Then methanol (15 ml) is added. The resulting crystals are collected by filtration, washed with a small amount of methanol and dried to give compound (2). -74- Example 6 : Synthesis of compound (43) To heterocyclic quaternary salt compound Q3 (3.75 g) were added methanol (25 ml), triethylamine (3.5 ml), dianyl compound A6 (1.95 g) and acetic anhydride (2.4 ml), and the mixture was stirred at room temperature for 1 hour. The 5 insoluble matter was filtered off, and a solution of sodium acetate (3.9 g) in a small amount of methanol was added to the filtrate. The mixture was stirred at room temperature for 1 hour. The resulting crystals were collected by filtration and washed with a small amount of methanol. To the crystals was added water (10 ml) for dissolution. Sodium acetate (2 g) was added, and then methanol (10 10 ml) was added. The resulting crystals were collected by filtration, washed with a small amount of methanol and dried to give 1.8 g of compound (43). The obtained compound (43) showed yellow in a flame test. .Maximum wavelength of absorbance (H2O): 773 nm .Molar absorption coefficient (H20): 204,000 15 .Maximum wavelength of fluorescence emission (H20): 789 nm Example 7 : Synthesis of compound (4) To heterocyclic quaternary salt compound Q3 (3.5 g) are added methanol (20 ml), triethylamine (3.5 ml), dianyl compound A7 (1.2 g) and acetic anhydride (1.9 20 ml), and the mixture is stirred at room temperature for 10 hours, and then stood overnight. The mixture is stirred under heating at 50°C for 5 hours. Water (2 ml) is added and the insoluble matter is filtered off. A solution of sodium acetate (5 g) in a small amount of water is added to the filtrate. The mixture is stirred at room temperature for 30 minutes. The resulting crystals are collected by 25 filtration and washed with a small amount of methanol and dried to give compound (4). Example 8 : Synthesis of compound (31) To heterocyclic quaternary salt compound Q4 (3.5 g) were added methanol (35 30 ml), triethylamine (3.5 ml) and acetic anhydride (2 ml), and dianyl comound A2 (1.8 g) was added portionwise with stirring. The mixture was further stirred for 1 hour. Acetic anhydride (2 ml) was added, and the mixture was stirred at Wo 00/16810 75 PCT/EP99/07088 room temperature for 5 hours. The insoluble matter was filtered off, and a solution of sodium acetate (4 g) in a small amount of methanol was added to the filtrate. The resulting crystals were collected by filtration and washed with a small amount of methanol. To the crystals was added water (10 ml) for dissolution. Then methanol (10 ml) was added, and the mixture was stirred at room temperature for 2 hours. The resulting crystals were collected by filtration, washed with a small amount of methanol and dried to give 1.3 g of compound (31). The obtained compound (31) showed yellow in a flame test. Maximum wavelength of absorbance (H2O): 755 nm Molar absorption coefficient (H20): 228,000 Maximum wavelength of fluorescence emission (H20): 774 nm The infrared absorption spectrum was measured for the obtained compound (31) by potassium bromide tablet method using a Fourier transform infrared spectrometer (VALOR-III, manufactured by JASCO). The following peaks were detected. The spectrum is shown in Fig. 12. IR (vmax(KBr)): 1518,1183,1149,1111, 995 cnV1 Example 9 : Synthesis of compound (41) To heterocyclic quaternary salt compound Q1 (12 g) were added methanol (120 ml), triethylamine (13.6 mi), dianyl compound A8 (4.4 g) and acetic anhydride (2.4 ml), and the mixture was stirred for 30 minutes. Acetic anhydride (2.4 ml) was added and the mixture was stirred for 1.5 hours, and then acetic anhydride (2.4 ml) was added and the mixture was stirred at room temperature for 6 hours. Heterocyclic quaternary salt compound Q1 (1 g), triethyi amine (3 ml) and acetic anhydride (3 ml) were further added and the mixture was stirred at room temperature for 2 hours. The mixture was stood overnight. Sodium acetate (5 g) was added and the resulting crystals were collected by filtration and washed with a small amount of methanol. To the obtained crude crystals was added water (200 ml). The insoluble matter was filtered off, and sodium acetate (10 g) was added to the filtrate. The resulting crystals were collected by filtration and washed with a small amount of WO 00/16810 76 PCT/EP99/07088 methanol. To the crystals were added water (200 ml) and triethylamine (10 ml), and a solution of sodium acetate (10 g) in methanol (100 ml) was added to give crystals. This step was repeated twice. The resulting crystals were collected by filtration, washed with a small amount of methanol and dried to give 9.7 g of compound (41). The obtained compound (41) showed yellow in a flame test. Maximum wavelength of absorbance (H20): 811 nm Molar absorption coefficient (H20): 230,000 Maximum wavelength of fluorescence emission (H2O): 822 nm Example 10 : Synthesis of compound (3) According to Example 5, heterocyclic quaternary salt compound Q3 and the corresponding dianyl compound are used to give compound (3). Example 11 in the same manner as in the synthesis of compound (29) in Example 1 except that potassium acetate (2 g) was used instead of sodium acetate (2 g), a compound that is the same as compound (29) except that the counter ion was potassium instead of sodium was obtained. Hereinafter this compound is referred to as compound (29) K salt The obtained compound (29) K salt showed purple in a flame test Maximum wavelength of absorbance (H2O): 780 nm Molar absorption coefficient (H20): 254,000 Maximum wavelength of fluorescence emission (H2O): 800 nm Other aforementioned compounds are treated in the same manner as in this Example to give compounds having potassium counter ion instead of sodium. These compounds having potassium counter ion are distinguished from the above compounds by attaching "K salt" after the corresponding compound numbers. WO 00/16810 77 PCT/EP99/07088 Example 12 In the same manner as in Example 11, compound (6) K salt was obtained. The obtained compound (6) K salt showed purple in a flame test. Maximum wavelength of absorbance (H2O): 788 nm Molar absorption coefficient (H2O): 226,000 Maximum wavelength of fluorescence emission (H2O): 806 nm Example 13 In the same manner as in Example 11, compound (2) K salt was obtained. The obtained compound (2) K salt showed purple in a flame test. Maximum wavelength of absorbance (H20): 743 nm Molar absorption coefficient (H2O): 266,000 Maximum wavelength of fluorescence emission (H2O): 762 nm Example 14 In the same manner as in Example 11, compound (4) K salt was obtained. The obtained compound (4) K salt showed purple in a flame test. Maximum wavelength of absorbance (H20): 753 nm Molar absorption coefficient (H20): 212,000 Maximum wavelength of fluorescence emission (H20): 767 nm Example 15 In the same manner as in Example 11, compound (3) K salt was obtained. The obtained compound (3) K salt showed purple in a flame test. Maximum wavelength of absorbance (H20): 751 nm Molar absorption coefficient (H20): 241,000 Maximum wavelength of fluorescence emission (H2O): 767 nm Example 16 The compound (6) K salt (50 mg) was dissolved in a small amount of water and passed through an ion exchange resin to convert potassium of the compound (6) K salt to proton. Thereto was added methanol saturated with WO 00/16810 78 PCT/EP99/07088 sodium acetate to allow precipitation of crystals. This procedure was repeated twice. The resulting crystals were collected by filtration, washed with a small amount of methanol and dried to give (32 mg) of compound (6). The obtained compound (6) showed yellow in a flame test. The infrared absorption spectrum was measured for the obtained compound (6) by potassium bromide tablet method using a Fourier transform infrared spectrometer (VALOR-III, manufactured by JASCO). The following peaks were detected. The spectrum is shown in Fig. 13. IR (vmax(KBr)): 1395, 1372,1188,1102,1020 cm"1 Example 17 : Synthesis of compound (54) To heterocyclic quaternary salt compound Q4 (3.5 g) were added methanol (20 ml), triethylamine (3.5 ml) and acetic anhydride (2 ml), and dianyl comound A1 (1.4 g) was added portionwise with stirring. The mixture was further stirred for 20 minutes. Acetic anhydride (1 ml) was added, and the mixture was stirred at room temperature for 1.5 hours. The insoluble matter was filtered off, and a solution of sodium acetate (4 g) in a small amount of methanol was added to the filtrate. The resulting crystals were collected by filtration and washed with a small amount of methanol. The crystals were dissolved in a small amount of water. Then the solution was diluted with methanol (10 ml), and the mixture was stirred at room temperature for 1 hour. The resulting crystals were collected by filtration, washed with a small amount of methanol and dried to give 1.5 g of compound (54). The obtained compound (54) showed yellow in a flame test. Maximum wavelength of absorbance (H2O): 743 nm Molar absorption coefficient (H20): 244,000 Maximum wavelength of fluorescence emission (H2O): 766 nm The infrared absorption spectrum was measured for the obtained compound (54) by potassium bromide tablet method using a Fourier transform infrared spectrometer (VALOR-III, manufactured by JASCO). The following peaks were WO 00/16810 79 PCT/EP99/07088 detected. The spectrum is shown in Fig. 14. IR (vmax(KBr)): 1511,1421,1099,1004, 926 cm'1 Experimental Example 1 The partition coefficient (log Po/w) of n-butanol/water was determined with respect to compound (29), compound (43), compound (45), compound (31), compound (3) K salt, compound (11) [available from Nippon Kankoh-Shikiso Kenkyusho CO., LTD. as NK-3261], compound (6) K salt, compound (2) K salt, compound (4) K salt, compound (34) and compound (54). As a control compound, used were NK-1967 (Nippon Kankoh-Shikiso Kenkyusho CO., LTD.) and ICG (Tokyo Kasei Kogyo) having only 2 sulfonic add groups in a molecule. The results are shown in Table 4. WO 00/16810 80 PCT/EP99/07088 Table 4 Compound Number of sulfonic acid group log Po/w (butanol/water) Compound (29) 6 -2.00 or less Compound (43) 5 -2.00 or less Compound (45) 5 -2.00 or less Compound (31) 4 -2.00 or less Compound (3) K salt 4 -2.00 or less Compound (11) (NK-3261) 4 -2.00 or less Compound (6) K salt 4 -2.00 or less Compound (2) K salt 4 -2.00 or less Compound (4) K salt 4 -1.51 Compound (34) 4 -1.49 Compound (54) 4 -2.00 or less NK-1967 cVch3 [ 11 ^—(CH=CH^-CH=/ J J 1 1 (CHzhSOa" (CHzhSOaNa 2 0.34 ICG ( Ic^CHs CH3 j 2 1.41 T jT>-{ch=ch)7ch==(j[ J 1 1 (CH^SOa* (CH2)4S03Na WO 00/16810 81 PCT/EP99/07088 Experimental Example 2 : Fluorescence imaging test (1) Tumor tissue pieces of mouse colon carcinoma (colon 26 carcinoma) were subcutaneously grafted to the left breast of BALB/c nude mice (5 weeks old, Clea Japan, Inc.). Ten days later when the tumor grew to a diameter of about 8 mm, the mice were subjected to the test. As a fluorescence excitation light source, a titanium sapphire laser was used. The test mice were uniformly exposed to the laser light using a ring type light guide (Sumita Optical Glass Co.) wherein dispersion of irradiation was within 10%. The irradiation power output was adjusted so that it was about 40 nW/cm2 near skin surface ofthe mice. The fluorescence was excited at the maximum excitation wavelength of each compound and fluorescence emission from the mice was detected and photographed through a short wavelength cutoff filter (IR84, IR86, IR88, Fuji Photo Rim CO., LTD.) with a CCD camera (C4880, Hamamatsu Photonics K.K.). The cutoff filter was selected to fit the excitation wavelength ofthe compound. The exposure time was adjusted depending on the fluorescence intensity of each compound. The test compounds used were compound (29), compound (31) and compound (6) K salt of the present invention, and NK-1967 and ICG having only 2 sulfonic acid groups in a molecule as control compounds. Each test compound (0.5 mg/ml) was dissolved in distilled water and administered to the mice via a tail vein. The dose was 5.0 mg/kg for compound (31), compound (6) K salt, NK-1967 and ICG, and 0.5 mg/kg for compound (29). At 24 hours after the administration of the compouds, the mice were anesthetized with diethyl ether and fluorescent light images of the entire body of the mice was photographed. The results are shown in Figs. 1 to 5. The compound (29) having a benzotricarbocyanine structure and six sulfonic acid groups, as well as compound (6) K salt and compound (31) both having a tricarbocyanine structure and four sulfonic acid groups obviously generated clearer images of tumor as compared to control compounds (NK-1967 having WO 00/16810 82 PCT/EP99/07088 benzotricarbocyanine structure and ICG having tricarbocyanine structure) having two sulfonic acid groups. In particular, compound (29) could clearly depict the tumor even at a low dose and was noticeably effective. Experimental Example 3 : Fluorescence imaging test (2) Nude mice were used for the test, compound (29) of the present invention and control compound ICG were intravenously injected from the tail vein at a dose of 5.0 mg/kg each under sevofiurane continuous inhalation anesthesia. At the same time, intermittent photographing of the fluorescence images was initiated. For photographing of the fluorescence images, exposure to excitation laser beam and extraction of fluorescence through a filter were done, wherein exposure time was one second. At 20 seconds after the administration of the compounds, blood vessel was suitably imaged. The fluorescence images were photographed until 5 minutes after the administration. Figs. 6 to 9 show fluorescence images of the entire body of the mice at 20 seconds and 5 minutes after the administration. ICG failed to contrastively show blood vessel in 5 minutes, whereas compound (29) couid image the blood vessel for longer time than ICG Experimental Example 4 : Residence in blood vessel In the same manner as in Experimental Example 2, tumor tissue pieces were grafted to CDFi mice (female, 5 weeks old, Japan SLC, Inc.), and about 2 weeks later when the tumor grew to a diameter of about 1 cm, the mice were subjected to the test. The test compounds were compound (29) K salt and compound (41) K salt having a benzotricarbocyanine structure and 6 sulfonic acid groups; compound (6) K salt, compound (4) K salt, compound (45) K salt, compound (31), compound (31) K salt, compound (3) K salt, compound (2) K salt, compound (43) K salt and compound (11) having a tricarbocyanine structure and 4-5 sulfonic acid groups; and control compounds ICG and NK-1967. Each test Wo 00/16810 S3 PCT/EP99/07088 compound was dissolved in distilled water (0.5 mg/ml) and used. The obtained each compound solution was administered from the tail vein ofthe mice (5.0 mg/kg). Blood was taken from the mice at 0.5,1,4 and 24 hours after the administration of the compounds and centrifuged to give plasma. The fluorescence intensity of the plasma was measured by a spectrofluorescence meter (RF 5300 PC, SHIMADZU CORPORATION). A calibration curve of each compound was drawn and compound concentration in plasma was calculated. The results are shown in Fig. 10. The compounds of the present invention remained in plasma at high concentration for a long time. Experimental Example 5 : Acute toxicity Reduction of toxicity by the introduction of sulfonic acid group and reduction thereof by conversion into sodium salt were studied. The test compounds were those listed in Table 5. Each test compound was dissolved in distilled water to give a compound solution. This solution was intravenously injected to the conscious mice from the tail vein. The mice were monitored for 3 days after the administration, and acute toxicity [LD50 (mg/kg body weight)] was estimated. The results are shown in Table 5. WO 00/16810 84 PCT/EP99/07088 Table 5 Number of sulfonic Compound LDso (mg/kg acid group body weight) compound (11)K salt 350 compound (11) 1980 compound (31 )K salt 350 compound (31) >3550 compound (31)Ca salt 2000 compound (31) Three or more pyridinium salt 1000-2000 compound (45)K salt 550 compound (45) 1100-1220 compound (43)K salt 300-350 compound (43) 1630 compound (41 )K salt 470 compound (41) >1010 compound (29)K salt 470 compound (29) >1010 compound (54) >5000 compound (6)K salt 350 compound (3)K salt 530 compound (4)K salt 450 compound (2)K salt 610 Two or less ICG 70 NK1967 20 An increase in the number of sulfonic acid group in a molecule or conversion to a sodium salt resulted in striking reduction of acute toxicity. WO 00/16810 85 PCT/EP99/07088 The near infrared fluorescent contrast agent of the present invention is excited by an excitation light and emits near infrared fluorescence. This infrared fluorescence is superior in transmission through biological tissues. Thus, detection of lesions in the deep part of a living body has been made possible. In addition, the inventive contrast agent is superior in water solubility and low toxic, and therefore, it can be used safely. </div>

Claims

<div class="application article clearfix printTableText" id="claims"> 86 WHAT IS CLAIMED IS:

1) The sodium salt of the formula ch3

ch3

NaQ3S

CH3

ch3 ch-

S03Na ch=ch-ch=c-ch=ch-ch

(ch&sof

(ch2)2s03Na

2) A near infrared fluorescent contrast agent comprising the sodium salt of claim 1.

3) The near infrared fluorescent contrast agent of claim 1 and 2. which is for tumor imaging.

4) The near infrared fluorescent contrast agent of claim 1 and 2, which is for angiography.

5) The sodium salt according to claim 1 substantially as herein described or exemplified.

6) A near infrared fluorescent contrast agent according to claim 2 substantially as herein described or exemplified.

SCHERING AKTIENGESELLSCHAFT AND FUJI PHOTO FILM CO., LTD. By Their Attorneys

</div>