NZ337056A - A 24 kD protein specific for binding to HCV epitotes which can be used for diagnosis and treatment of HCV related diseases - Google Patents

A 24 kD protein specific for binding to HCV epitotes which can be used for diagnosis and treatment of HCV related diseases

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Publication number
NZ337056A
NZ337056A NZ337056A NZ33705699A NZ337056A NZ 337056 A NZ337056 A NZ 337056A NZ 337056 A NZ337056 A NZ 337056A NZ 33705699 A NZ33705699 A NZ 33705699A NZ 337056 A NZ337056 A NZ 337056A
Authority
NZ
New Zealand
Prior art keywords
protein
hcv
binding
subjecting
cells
Prior art date
Application number
NZ337056A
Inventor
Sergio Abrignani
Original Assignee
Chiron S
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GBGB9517926.3A external-priority patent/GB9517926D0/en
Application filed by Chiron S filed Critical Chiron S
Publication of NZ337056A publication Critical patent/NZ337056A/en

Links

Abstract

A use of a protein having a molecular weight of 24kD and capable of specifically binding to a protein of hepatitis C virus for treating HCV infection. The method of preparing the protein is characterised by: 1. Culturing plasma cells exhibiting binding to a HCV protein and purifying the protein from the preparation of the cells; 2. The cells are selected and cloned to provide for overexpression of the protein; 3. Subjecting the preparation to ammonium sulphate precipitation to ammonium sulphate at less than 33% saturation and retaining the supernatant; 4. Subjecting the supernatant to an ammonium sulphate precipitation (33 and 50%) purification step and retaining the precipitate; 5. Resuspending the precipitate and subjecting it to hydrophobic interaction chromatography. Figure 1 is a schematic diagram describing an assay in which HCV receptor-binding ligands bind to receptors on HCV receptor target cells and are measured by first binding a labeled anti-rabbit IgG-FITC F(ab') fragment prior to cell separation by FACScan analysis.
NZ337056A 1995-09-01 1999-08-04 A 24 kD protein specific for binding to HCV epitotes which can be used for diagnosis and treatment of HCV related diseases NZ337056A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB9517926.3A GB9517926D0 (en) 1995-09-01 1995-09-01 Binding protein
NZ31618699 1999-08-04

Publications (1)

Publication Number Publication Date
NZ337056A true NZ337056A (en) 2001-03-30

Family

ID=26307679

Family Applications (1)

Application Number Title Priority Date Filing Date
NZ337056A NZ337056A (en) 1995-09-01 1999-08-04 A 24 kD protein specific for binding to HCV epitotes which can be used for diagnosis and treatment of HCV related diseases

Country Status (1)

Country Link
NZ (1) NZ337056A (en)

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