NZ244363A - Phosphonate esters - Google Patents

Description

New Zealand Paient Spedficaiion for Paient Number £44363 24 4 3 6 3 Under the provisions ©? Regulation 23 {*) the L.MU!.: -Specification has boon tjc.

Priority Datc(s;: "to, Compile Specificatirr.!.

Ch«sS; 21 SEP m Publication Date: P.O. Journal, No: .... 13 Initials NO DRAWINGS NEW ZEALAND PATENTS ACT, 1953 16 SEP 1992 ft so LiVZJ Divided out of No.: 234502 Date: 13 July 1990 COMPLETE SPECIFICATION PHOSPHORUS-CONTAINING COMPOUNDS We, E R SQUIBB & SONS, INC., of Laurenceville-Princeton Road, Princeton, New Jersey, United States of America, a corporation organised under the laws of the State of Delaware, United States of America, hereby declare the invention for which we pray that a patent may be granted to us, and the method by which it is to be performed, to be particularly described in and by the following statement:- - 1 -(followed by page la) 24 4 3 - la - PHOSPHORUS-CONTAINING COMPOUNDS The present invention relates to new intermediates in the preparation of phosphorus-containing compounds which are useful in inhibiting cholesterol biosynthesis by inhibiting de novo squalene production.

Squalene synthetase is a microsomal enzyme which catalyzes the reductive dimerization of two molecules of farnesyl pyrophosphate (FPP) in the presence of nicotinamide adenine dinucleotide phosphate (reduced form) (NADPH) to form squalene (Poulter, C. D.; Rilling, H. C., in "Biosynthesis of Isoprenoid Compounds", Vol. I, Chapter 8, pp. 413-441, J. Wiley and Sons, 1981 and references therein). This enzyme is the first committed step of the de novo cholesterol biosynthetic pathway. The selective inhibition of this step should allow the essential pathways to isopentenyl tRNA, ubiquinone, and dolichol to proceed unimpeded. Squalene synthetase, along with HMG-CoA reductase } /- -/ a H .j U has been shown to be down-regulated by receptor mediated LDL uptake (Faust, J. R.; Goldstein, J. L.; Brown, M. S. Proc. Nat. Acad. Sci. USA, 1979, 76, 5018-5022), lending credence to the 5 proposal that inhibiting squalene synthetase will lead to an up-regulation of LDL receptor levels, as has been demonstrated for HMG-CoA reductase, and thus ultimately should be useful for the treatment and prevention of hypercholesterolemia 10 and atherosclerosis.

One approach to inhibitors of squalene synthetase is to design analogs of the substrate FPP. It is clear from the literature that the pyrophosphate moiety is essential for binding to 15 the enzyme. However, such pyrophosphates are unsuitable as components of pharmacological agents due to their chemical and enzymatic lability towards allylic C-0 cleavage, as well as their susceptibility to metabolism by phosphatases. 20 P. Ortiz de Montellano et al in J. Med. Chem., 1977, 20, 243-249 describe the preparation of a series of substituted terpenoid pyrophosphate (Table A), and have shown these to be competitive inhibitors of the squalene 25 synthetase enzyme. These substances retain the unstable allylic pyrophosphate moiety of FPP.

C H "i 0 3- Table A z y o p-o" o" z h h h h h sch3 Corey and Volante, J. Am. Chem. Soc. 1976, 98, 1291-3, have prepared FPP analog A and presqualene pyrophosphate (PSQ-PP) analog B as inhibitors of squalene biosynthesis. (Presqualene pyrophosphate is an intermediate in the conversion of FPP to squalene). These inhibitors possess methylene groups in place of the allylic oxygen moiety of FPP and PSQ-PP, but still retain the chemically and enzymatically unstable pyrophosphate linkage.

X^ 0 0 0 \ . / \I. p o" P— 0 o" A FPP X = CH, X = 0 PSQ-PP X = 0 Poulter and co-workers have prepared 25 cyclopropane C (Sandifer, R. M. , et al., J. Am. Chem. Soc. 1982, 104, 7376-8) which in the presence of inorganic pyrophosphate is an intermediate analog inhibitor of the enzyme squalene synthetase.

Altman and co-workers, Bertolino, A., et al., Biochim. Biophys. Acta. 1978, 530, 17-23, 15 reported that farnesyl amine and related derivatives D inhibit squalene synthetase, but provide evidence that this inhibition is non-specific and probably related to membrane disruption. r = h, ch2ch2oh, ch2ch2och3 D Poulter, C.D., et al, J. Org. Chem., 1986, 51, 4768, prepared compound E in a demonstration of a synthetic method, but did not report any biological data. 24 4 3 6 6- Poulter, C.D., Stremler, K.E., J.A.C.S., 10 1987, 109, 5542 describes the synthesis and biological evaluation of compounds having structure F. These compounds were evaluated as alternative substrates for avian liver and lemon peel farnesyl diphosphate cyclase. 0 0 ! I 11 O-P-X-P-OH 1 I OH OH F X=CH2, CF2 McClard, R. W. and Poulter, C. D., et al., J.A.C.S. 1987, 109, 5544, reported that phosphinylphosphonates G and H were competitive 25 inhibitors of the 1'-4-condensation between isopentenyl diphosphate and geranyl diphosphate catalyzed by avian liver farnesyl diphosphate synthetase. Phosphinylphosphonates G and H had Ki's of 19pM and 71pM, respectively. They also 4 4 3 6 3 reported the speculative isolation of the farnesyl phosphinylphosphonate I_, and the geranyl phosphinylphosphonate J from the enzymatic reaction of G with geranyl pyrophosphate or dimethylallyl pyrophosphate, respectively. The structures of I_ and J were tentatively assigned based on relative TLC mobilities. They hypothesized that I_ could be a potential inhibitor of squalene synthetase. i 24 4 3 Capson, T.L., PhD dissertation, June 1987, Dept. of Medicinal Chemistry, the University of Utah, Abstract, Table of Contents, pp. 16, 17, 40-43, 48-51, Summary, and T.L. Capson, C.D. Poulter et al, J. Org. Chem., 1988, 53, 5903-5908 disclose cyclopropanes of the structure K H H K OO II II R=-H or -(CHo),~P-0-P-0* 2. 1 | | O" O* as intermediate analog inhibitors of squalene 35 synthetase. 24 Biller and coworkers, "Isoprenoid (Phosphinylmethyl)phosphonates as Inhibitors of Squalene Synthetase," J. Med. Chem., 1988, 31, 1869, synthesized analogues of FPP, 2a-d and 3a,b, 5 where the allylic and anhydride oxygen atoms are replaced with carbon. The PMP subunit thereby serves as a stable surrogate for the diphosphate. They demonstrate that isoprenoid (phosphinylmethyl)-phosphonates (PMPs) are effective inhibitors of 10 squalene synthetase, binding to the enzyme with affinity comparable to FPP itself. 0 0 l ii It R -P-CH--P-0 0 0 i _ ' — 0 0 I - ^ 1 O 0 2a-d 3a, b R 1 24 f i u •rt i S 4 4 v-' V - 10 In accordance with the invention in New Zealand Patent Specification No. 234502, there is provided phosphorus-containing compounds which inhibit cholesterol biosynthesis, and thus are useful as hypocholesterolemic and antiathero-sclerotic agents and have the following structure 0 Y1 0 1 11 ' " 3 I. R — (CH~ ) —X—- (CEL ) —P C P OR 2 n 2 m i 0*9 '4.

OR Y OR wherein mis 1, 2 or 3; n is 0, 1, 2 or 3; Y and 2 2 3 Y are H or halogen, preferably H or F; R , R and 4 R are independently H, metal ion, to CQ alkyl or to C12 alkenyl; X is 0, NH, -N- or S (wherein CH-R R15 is H or C, to CR alkyl); R1 is R5-Q1-Q2-Q3- 1 z 3 wherein Q , Q and Q are independently: R7 R6 R8 R9 iii i -CH—C=C—CH2-, -CH2-CH-CH2-CH2-, -CH2-C=C-CH2-, or a bond, with the stipulation that if Q"1" is a 2 3 2 bond, then Q and q must be bonds, and if Q is a O £ bond, then Q is a bond; R is H, lower alkyl, halo 7 or haloalkvl (e.g. CH2F, CF-); R is H, halogen, lower alkyl or alkylthio; R is H, halogen, tri-methyl: alkyl; Q methylsilyl or lower alkyl; R is H, or lower rnr12 r13 ^ in11 14 1 R is Rlu-c=c—ch2~, r1^-^—ce2-ce2-, / /• X fx ^ „ T U R^-CHC-C^-(wherein is lower alkyl or H), or CH3(CH2)p- where p is 2 to 7; R10 and R11 are independently hydrogen, lower alkyl such as methyl or ethyl, halogen, lower alkenyl or haloalkyl or R^ and R^ can be taken together to form (CH2)s, where s is 2 to 7; R12 is hydrogen, lower alkyl, halogen or lower alkenyl; R13 and R14 are independently lower alkyl such as methyl or ethyl; with the proviso that if all of Q1, Q2 and Q3 are bonds, then both R10 and R11 cannot be H, and R5 cannot be CH^CH^^-, with p < 4.

The formula I compounds include all stereoisomers thereof.

Unless otherwise indicated, the term "lower alkyl" or "alkyl" as employed herein alone or as part of another group includes both straight and branched chain hydrocarbons containing 1 to 8 carbons in the normal chain, preferably 1 to 4 carbons, such as methyl, ethyl, propyl, isopropyl, butyl, t-butyl, isobutyl, pentyl, hexyl or isohexyl.

The term "lower alkenyl" or "alkenyl" as used herein by itself or as part of another group refers to straight or branched chain radicals of 2 to 12 carbons, preferably 3 to 6 carbons in the normal chain, which include one double bond in the normal chain, and which may include an aryl or alkyl substituent, such as vinyl, 2-propenyl, 2-butenyl, 3-phenyl-2-propenyl, 2-pentenyl, 2-hexenyl, 2-heptenyl, 2-octenyl, 2-nonenyl, 2-decenyl, 2-undecenyl, 2-dodecenyl and the like.

The term "halogen" or "halo" as used herein refers to chlorine, bromine, fluorine, and iodine with chlorine or fluorine being preferred. 9 ^ /> X R l. ~s *°i 0 0 The term "haloalkyl" as used herein refers to any of the lower alkyl groups defined above substituted with a halogen as defined above, for example CH2F, CF3 and the like.

The term "metal ion" refers to alkali metal ions such as sodium, potassium or lithium and alkaline earth metal ions such as magnesium and calcium.

Preferred are those compounds of formula I which have the following formula: ia O Y1 0 12 3 11 ' 11 _ R -Q -Q -Q -( ch, ) -x- ( ch, ) -p—c—p-o 2 n 2m i i7i o" Y 0" wherein R5 is CH3-C=CH-CH2-; <=H3 3 Q is a bond; Q2 is -CH-.-C=CH-CH_-; -CH_-C=C-CHn-; -CH_-CH=CH-CH--2 i 2 2 2 2 2 CH_ 1 Q = -CH_-C=CH-CH„-2 i 2 CH_ 1 n is 0 or 1; m is 1 or 2; X is 0 and Y and 2 Y are each H or F.

The compounds of formula I may be prepared according to the following reaction sequences.

• • • • Compounds of formula I wherein X is 0, S or Nil may he prepared according to the following reaction sequence: Scheme I 0 1 1 '' 2a R -(CH„) -X -(CH,) -P-OR 2 n 2 m | OR IIA (X =0,S or N-Pro) (R 2a is C^-Cg alkyl or C3-Ci2 alkeQy1) Scheme IA IIA (X^O.S) A. Oil (Hydrolysis) or B. NaCN, DMSO or C. Nal, DMF or mono- dealkylation 0 Rl-(CII0) ^-(CIL) -P-OJI 2 n 2 m | OR 11 IA (Xl=0,S or N-Pro) D. 1. TMSBr 2. R2aOH, DCC, bisdealkylation • and reesterification pyridine (D. used where Xj^N-Pro) -*► Q 1 2 11 R -(Cll ) -X -(Cll ) -P-OH 2 n 2 in i _ OR (X2=0,S) 11 IB OJ l ro cr> Os| Scheme II 0 I. (C_H,)_NSi(CH ) il 2 5 2 3 3 R -(CH_) -X -(CH„) -P-OH 2. (COC1)., DHF 2 n 2 m | _ 2 OR"3 0 IIIA (X]=0 or S) 3. ®M0CY1Y2P-OR3a ' 4a or XII TMSBr or TMSI 2,4,6-collidine 0 Y1 Y20 CH CI 1 2 " s / '' 3a 2 2 R -(CH„) -X -(CH„) -P C P-OR z n 2 m , , OR23 ORA 2. e0H IA (where X2=0 or S) Strong 0 Y1 Y20 1 2 'I v / II ID b> R -(CH_) -X -(CH-) -P C—P-OH ^ 2 n 2 ra | j acid OH OH IE (X2=0,S) . • acid chloride - formation ^ IA a-phosphonate anion P-C-P coupling 0 R1-(CH0)_-X2-(Cll ) -P- ,1 2 Y 0 2 n 2 in | 0 e \ / —c—p-o J)e e ID (X =o,s) rv> wis ,4>- o*> Scheme III 0 DCC, DMAP, Pyridine 0 1 1 " 1 1 / \ R -(CH_) -X -(Cll,) -P-OH p-NO,phenol R -(CH„) -X -(C1L) -P-O-/ O / N°o z n z m - / z n z m ■ \ / z OR23 ^ OR23 (X^=0,S,N-Pro) Esterification IV (X^=0,S,N-Pro) IIIA T1IF 0 Y1 Y20 1 1 i' \ / I' -ia IV !► R -(CH_) -X -(CH_) -P C P-OR 2 n 2 m 1 2a 1 4a « O • OR OR h-* 1 Q | ill Vcv y for 1 0R^a (PCP coupling) IB (X^=0, S or N-Pro) VII Scheme IV 1). TMSI, Collidine 1 2 (Deprotection) O Y Y 0 1 w 1 ii \ 6 IB (where X =NPro) ^ R (CH„) -N-(CH0) -P C P—0° 2n|2m| , 2). OH (Hydrolysis) 11 0 0 IC ro .£> O*' CM Scheme V R15CHO alcoholic solvent 1 2 or acetonitrile 0 Y Y 0 ► 1 II H o R -(CH_) -N-(CIL) -P C P-0° I ft I Q NaBH3CN, PH 3 to 8 CH, 0° 0° IC1 R15 I to CM In accordance with the present invention there is provided new intermediate compounds for the preparation of the compounds of formula I, namely compounds having the structure 0 Rl-<CH2>n-x-<CH2>m-?-?fa OR u R1-(CH2)n-X1-(CH2)m-P-0:/o)- NO- OR2^ Rl-<CH2)n-X1-<CH2),0-P-OH or OR23 0 Rl-(CH2)n-X2-(CH2)m-P-Cl OR wherein n is 0, 1, 2 or 3; m is 1, 2 or 3; X is 0, S, NH or N-Pro; XI is 0, S or N-Pro and Pro is a nitrogen protecting group; X2 is 0 or S; R2a is C,-CQalkyl or C_ C,-alkenyl; and R1 is 1 2 3 T 7 T R -Q -Q -Q - Wherein Qx, Q and QJ are independently: R7 R6 R8 R9 iii i -CH—C=C-CH2-, -CH-CH-CH2-CH2-( -CH2-C=C-CH2- or a bond, with the stipulation that if Q1 is a 2 3 ? bond, then Q and Q must be bonds, and if Q is a bond, then Q3 is a bond; R^ is H, lower alkyl, 8 halo or haloalkyl; R is H, halogen, trimethylsilyl or lower alkyl; R is H, halogen, lower alkyl or lower alkylthio; R^ is H or lower alkyl; and (kli & a 3 R R . 10 1 1 R is R1U-C=C-CH2- , R13 14 1 R-C-CH2-CH?- R16-C=C-CH2- or ,10 . „11 CH3(CH2)p- where p is 2 to 7; R and R are independently H, lower alkyl, halogen, lower alkenyl or haloalkyl; or R^° and can be taken together to form (CEL) where s is 2 12 to 7; R is H, lower alkyl, halogen or lower 13 14 alkenyl; R and R are independently lower alkyl; and 16 R is lower alkyl or H; with the proviso that if all of Q1, Q2 and Q3 are bonds then R^"° and R^ cannot be H, and R^ cannot be CH3(CH2)p- where p is less than or equal to 4.

As seen in Reaction Scheme I, compounds of formula I where X is 0, S or NH may be prepared in accordance with the following method of the invention starting with diester IIA (which is a new intermediate). 0 1 1 " 2a IIA R -(CH_) -X -(CH-) -P-OR 2 n 2 m i - OR wherein X^" is 0, S or N-Pro and Pro is a nitrogen protecting group such as t-butyloxycarbonyl (t-BOC) or benzyloxycarbonyl (CBZ).

The diester IIA may be converted to the corresponding monoester IIIA (which is a new intermediate) 0 IIlX RMcH2)n-X^CH2)ra-P-OH OR h by any of four methods (A, B, C or D) as discussed below. Methods A, B and C apply where X^" is 0, S or N-Pro and Method D applies where is 0 or S.

In Method A, diester IIA is treated with a strong aqueous base such as NaOH, KOH or LiOH, typically in the presence of a solvent such as dioxane, isopropanol, methanol or ethanol at a temperature within the range of from about 25 to about 125°C to form monoester IIIA.

In Methods B and C of the invention, diester IIA is subjected to a monodealkylation by treatment with sodium cyanide, potassium cyanide or lithium cyanide and a solvent such as dimethyl sulfoxide or dimethylformamide (Method B) or with sodium iodide, lithium iodide or lithium chloride in the' presence of a solvent such as dimethylformamide, dimethyl sulfoxide or acetone, the above reactions being carried out at a temperature of within the range of from about 40 to about 160°C, to form monoester 11 IA.

In Method D of the invention (Reaction Scheme IA), diester IIA is subjected to a bisdealkylation by treating IIA with bromotrimethyl-silane under an inert atmosphere such as argon in the presence of 2,4,6-collidine or triethylamine in dichloromethane and then reesterifying by 2a reacting with an alcohol (R OH) in the presence of dicyclohexylcarbodiimide (DCC) and an organic base such as pyridine, or 4-dimethylaminopyridine (DMAP) 2 to form monoester IIIB where X is 0 or S (which is a new intermediate). 0 IIIB R1-(CH_) -X2-(CH„) -P-OH ^ n 2, m i o ^ OR As seen in Reaction Scheme II, compounds of formula I where X is O or S may be prepared in accordance with the following method of the invention starting with monoester IIIA where X1 is 0 or S or 2 IIIB where X is 0 or S which is dissolved in an inert organic solvent such as dichloromethane and treated, under an inert atmosphere such as argon, with diethyl(trimethylsilyl)amine. After evaporation of solvent, the residue is dissolved in dichloromethane or an aromatic solvent such as benzene or toluene, or other appropriate inert organic solvent, preferably containing dimethylformamide as a catalyst, under an inert atmosphere such as argon, and oxalyl chloride is added thereto. The reaction mixture is evaporated to give acid chloride V (which is a new intermediate) 0 V R1-(CH_) -X2-(CH-)-P-Cl (X2 is 0 or S) £» n 2a HI J « OR where R2a is C^-Cg alkyl or C3~C12 alkenyl.

An a-phosphonate anion P-C-P coupling is carried out on the acid chloride V as follows.

To a stirred solution of an optionally substituted dialkyl methyl phosphonate Y1 0 i i1 VI H—C—P—OR Y2 0R4a 1 2 wherein Y , and Y are as defined hereinbefore and R3a and R4a are independently C^-Cg alkyl or C3~C12 alkeny1' in an inert organic solvent such as tetrahydrofuran cooled to a temperature within the range of from about -90°C to about 0°C is added a strong base, such as n-butyl lithium or lithium diisopropylamide, in hexane, tetrahydrofuran or other inert organic solvent under an inert atmosphere such as argon, followed in some instances 2 4 4 3 6 by transmetallation by the addition of a metal halide, such as CeCl3< ZnC^/ MgB^, Cul, to form the metal salt VII VII Y1 0 M®GC—P-OR3a I I Y OR 4a wherein M is Li+, Na+, K+, +MgHal, +ZnHal, +Ce(Hal), + or Cu wherein Hal is a halogen ion such as Cl", Br" or I~.

The metal salt VII is maintained at a reduced temperature as described above and acid chloride V in an inert organic solvent such as tetrahydrofuran or diethyl ether is added to form the phosphinyl-phosphonate IA.

IA R1-(CH2)n-X2-(CH2)m-p.

OR 1 2 0 Y Y 0 " \ " 3a —C P—OR 2a OR 4a (X =0,S) The metal salt VII will be employed in a molar rat}.o to acid chloride V of within the range of from about 1.0:1 to about 2.5:1 and preferably from about 1.8:1 to about 2.4:1. Triester IA, in an inert organic solvent such as methylene chloride, may then be subjected to dealkylation by treating with excess bromotrimethylsilane or iodotrimethyl-silane in the presence of 2,4,6-collidine or bis(trimethylsilyl)trifluoroacetamide and then treating with a strong inorganic base such as aqueous NaOH, KOH, LiOH or Mg(OH)2> optionally in the presence of an alcohol such as methyl alcohol, to form the salt ID which may be separated out by chromatography. Salt ID may be treated with a strong acid such as HCl to form acid IE. v •••'• /. \ ...• 7 (L T "a' U 0 'J 1 2 0 Y Y 0 1 ? 11 \ / " a ID R -(CH_) -X -(CH_) -P C P-0 d. n i m i , O0 O0 1 2 0 Y Y 0 IE 1 2 ii \ / i' R -(CH,)-X-(CH_) -P C P-OH il ill | I OH OK As seen in Reaction Scheme III, compounds of 10 formula I where X is 0, S or NH may be prepared according to the following method of the invention starting with monoester I IIA (X^O, S, N-Pro) which is dissolved in pyridine, and treated with p-nitro-phenol and 4-dimethylaminopyridine and dicyclohexyl-15 carbodiimide under an inert atmosphere such as argon at 25-60°C (employing a molar ratio of phenol:IIIA of within the range of from about 0.8:1 to about 1.2:1) to form the p-nitrophenyl ester IV (which is a new intermediate) 0 > , IV Ri.(cH2)n-x1-(ca2)m-P-o-Yo)-I,02 OR ^ ' (where X^" is 0, S or N-Pro) An a-phosphonate anion P-C-P coupling is carried out on nitrophenyl ester IV by reacting nitrophenyl ester IV with metal salt VII in a manner similar to that described above for IA to form the 30 phosphinyl-phosphonate IB 24 4 36 3 1 2 O Y Y 0 1 1 x \ " 3 IB R -(CH, ) -X -(CH, )m-P C P-OR 2 n 2 m 1 2a '4a OR OR (X1 is 0, S or N-Pro) The metal salt VII will be employed in a molar ratio to p-nitrophenyl ester IV of within the range of from about 1.0:1 to about 2.5:1 and preferably from about 1.8:1 to about 2.4:1. 10 Triester IB, (X^=0,S) is identical to IA above, and may be subjected to dealkylation as described for IA to form ID and IE.

As seen in Reaction Scheme IV, compounds of formula IC wherein X is -N- may be prepared 15 H according to the following method of the invention by treating compound IB where X"'" is N-Pro with a solution of iodotrimethylsilane, and 2,4,6-collidine or bis(trimethylsilyl)trifluoroacetamide 20 followed by treating with strong alkali metal base, such as NaOH, KOH or LiOH to form the corresponding salt IC.

In Reaction Scheme V, compounds of formula I wherein X is -N- may be prepared by treating 1 15 CH,-R 15 • compound IC with aldehyde (R CHO wherein R is H or C^ to C^ alkyl) in the presence of an alcoholic solvent such as ethanol or acetonitrile at a pH of 3 to 8 to form IC"*".

The triesters IA or IB (X=0,S) may be hydrolyzed to the corresponding monoester IJ as follows.

O / / a / a A ^ n : 3 v,-' 1 2 Triester IA or IB where X and X are 0 or S may be treated with strong inorganic bases such as KOH, NaOH or LiOH in ^0 or ^O/alcohol mixtures, or with nucleophiles such as, NaCN, KCN, Nal, LiCl, 5 or LiBr in dimethylformamide or dimethylsulfoxide, under an iner~ atmosphere such as argon, employing a molar ratio of base or nucleophile to triester of within the range of from about 2:1 to about 10:1, and at a temperature within the range of from about 10 25° to about 160°C to form the monoester IJ 1 2 0 Y Y 0 IJ R1-(CH,) -X-(CH,) -P——C P-OR3d 2 n 2 m i i 0~M O"M Si 8. where M is an alkali metal. 1 Triester IB where X is N-Pro may be hydrolyzed to the monoester IK by treating IB (X"*"=N-Pro) with p-toluenesulfonic acid in refluxing 20 benzene or trifluoroacetic acid at -20°C to 25°C to remove the N- protecting group and then hydrolyzing as described above with respect to triester IA and IB to form monoester IK O Y1 Y20 1 11 N ' l! 3a IK R -(CH-) -N-(CH-) -P C P-OR 2 n i 2m, , H 0~M 0~M 8 cl In preparing compounds wherein X is NH, the 30 nitrogen atom in the starting material may be protected by treating a solution of amine IIC (which is a new intermediate) 24 4 3 6 IIC R1-(CHo)„-N-(CH,)m-P-0R2a 2 n 2 m i - H OR with an inert organic solvent such as methylene chloride, under an inert atmosphere, with a protecting reagent such as di-t-butyl dicarbonate, or benzyl chloroformate, optionally in the presence 10 of an amine base such as triethylamine or pyridine, to form the protected starting material IID (which is a new intermediate) 0 1 "2a IID R -(CH-) -N-(CH- ) -P-OR 2'n i 2'm , - Pro OR 0 0 ii i1 where Pro can be t-C^HgO-C-, or CgH^-CE^-O-C- The starting material IIA may be prepared in accordance with the following method of the invention starting with compound X X R1-(CH-) -XXH 2 n where X^ is 0, S or N-Pro which is made to undergo carbene insertion by treating a solution of X in dry deoxygenated solvent such as deoxygenated 30 benzene under an inert atmosphere such as argon 0 h with Rh2(OCCH3)4 and then with phosphonate XI 2 4 A 7> JU. i i %s 0 h 2a XI N,CH-P-OR 1 ?a OR (prepared as described by Seyferth, D. et al, J.O.C. 1971, 36_, 1379) in dry deoxygenated solvent as described above to form compound IIA.

In carrying our the above carbene insertion 10 compound X is employed in a molar ratio to phosphonate XI of within the range of from about 1:1 to about 1:3 and preferably about 1:2.

In a preferred method, the starting material IIA wherein R2a is alkyl, m is 1, n is 0 15 and X^" is 0 may be prepared by reacting farnesyl chloride with phosphonate alkoxide XI a 0 XIa (R2a0)2?CH2-O9alkali metal®.

In another preferred method, in accordance with the following method of the invention, starting material IIA where X^" is 0 or S and IIC where X is NH, may be prepared by alkylating XA XA R1(CH2)n-XH where X is 0, S or NH, by treating XA in an inert organic solvent such as tetrahydrofuran, diethyl ether or benzene with a base such as n-butyllithium, 30 NaH or ((CH3)^-Si)2NLi when X is 0 or S or a trialkylamine base such as triethylamine or di-isopropylethylamine when X is NH followed by treatment with XII 0 /' 4 6 £ 0 XII Z-(CH,) -P-0R2a 2 m ' 2a OR where Z=p-CH3CgH5S03- or CF3S03-. The reaction is carried out under argon within the range of from about -78°C to about 25°C where X is 0 or S to form IIA (X^=0,S) and from about -20°C to about 80°C 10 where X is NH, to form IIC (X=NH).

In carrying out the above alkylation XA will be employed in a molar ratio to phosphonate XII of within the range of from about 2:1 to about 0.5:1 and preferably from about 0.9:1 to about 1.1:1.

The phosphonate XII where m is 1 may be prepared by treating phosphite XIII XIII (R2a0)2-P-0H with paraformaldehyde and organic base such as triethylamine at a temperature within the range of from about 70 to about 120°C under an inert atmosphere such as nitrogen to form compound XIV 0 2 a 11 XIV R O-P-CH-OH 7a1 2 R 0 Compound XIV is dissolved in a suitable dry organic solvent such as diethyl ether, tetrahydrofuran or toluene and cooled to a temperature within the range of from about 25°C to about -80°C and then is treated with organic base such as diisopropylethyl amine, triethylamine and pyridine, optionally containing 4-dimethylaminopyridine, and then trifluoromethanesulfonic anhydride or p-toluenesulfonyl 5 chloride in a suitable organic solvent such as diethyl ether, dichloromethane or pyridine to form phosphonate XII where m is 1.

The above reaction is carried out employing a molar ratio of XIII: paraformaldehyde of within 10 the range of from about 0.8:1 to about 1.2:1 and a molar ratio of XV to trifluoromethanesulfonic anhydride or tosyl chloride of within the range of from about 0.8:1 to about 1.2:1.

Phosphonate XII where m is 2 or 3 may be 15 prepared by treating alcohol XV XV Hal-(CH2)tCH2OH where Hal is CI, Br, or I and t is 1 or 2, with 20 aihydropyran, employing a molar ratio of dihydropyran:XV of from about 2:1 to about 1:1, in the presence of an inert organic solvent such as methylene chloride, chloroform or toluene and catalytic amounts of p-toluenesulfonic acid or 25 pyridinium p-toiuenesulfonate at temperatures of from about 0° to about 25°C to form tetrahydro-pyranyl ether XVI XVI Hal(CH2)tCH20-^ 0 Tetrahydropyran XVI is treated with phosphite XVII (in a molar ratio of XVII:XVI of from about 20:1 to about 3:1) at 70-160°C to form phosphonate XVIII which is treated with acid such as pyridinium p-toluenesulfonate or p-toluenesulfonic acid in an alcohol solvent such as ethanol to form the 15 phosphonate XIX Phosphonate XIX is then treated with organic base and trifluoromethanesulfonic anhydride or p-toluene-sulfonyl chloride (as described above in forming 25 phosphonate XII where m is 1) to form phosphonate XII where m is 2 or 3.

Compound IIA (X=0,S) where m is 2 may be prepared by a Michael addition to a vinyl phosphonate by treating a solution of compound XA 30 (where X is 0,S) and tetra-n-butyl-ammonium fluoride catalyst in tetrahydrofuran or other solvent such as benzene under an inert atmosphere such as argon XVII P(OR2 a)3 XVIII 24 4 3 with a vinyl phosphonate XX (employing a molar ratio of XA:XX of from about 0.8:1 to about 1.2:1) XX 0 11 2a CH-.=CH-P-OR l OR 2a to form compound IIA where m is 2.

Compound IIC where m=2 is prepared by 10 treating XA (X=NH) with XX (0.9-1.2 equiv) in alcohol solvent (for example, CH3OH) at from 25°C-80°C.

Compound IIA where X is S and where m is 1 may be prepared starting with compound XXI 0 0 11 " 2a XXI CH3CSCH2-P-0R (prepared as described by Farrington, G.F. et al, J. Med. Chem. 1988, 28, 1968) which is reacted with sodium ethoxide in ethanol followed by halide XXII XXII R1-(CH0) -Hal 2 n (employing a molar ratio of XXI:XXII of from about 2:1 to about 1:1).

Compound IIA where X is S and m is 2 or 3 may be prepared starting with phosphonate XXIII 0 " 2a XXIII HO-(CH-) -P-OR 2 m 1 2a OR (where m is 2 or 3) 2 4 4 3 6 3 which is subjected to a Mitsunobu coupling by treating XXIII with diisopropyl- or diethyl azodicarboxylate (DIAD, DEAD, resp.) and triphenyl phosphine in the presence of 0 ii CH3C SH to form XXIV 0 0 11 11 2a XXIV CH-CS(CH_) -P-OR 2 m ' 2a OR whi'ch is treated with sodium ethoxide in ethanol and then halide XXII (as described above) to form IIA where X is S and m is 2 or 3.

Compounds of formula IIA where m is 3 and X is 0 or S may be prepared starting with halide XXV XXV R1-(CH-) -X-CH-CH--Hal 2 n 2 2 where X is 0 or S, and Hal is preferably Br or I, which is subjected to an a-phosphonate anion 25 alkylation by treating XXV with phosphonate anion XXVI 0 XXVI L? 9CH-P-0R2a iR2* (molar ratio of XXV:XXVI of from about 1:2 to about 1:1) in an inert organic solvent such as tetrahydrofuran or diethyl ether to form compound IIA where m is 3 and X is 0 or S. 24 4 3 6 Halide XXV where X is 0 or S may be prepared by treating alcohol or thioalcohol XG XG R1-(CH2)n-X2-H 2 where X is 0 or S with sodium hydride in the presence of an inert organic solvent such as tetrahydrofuran, and the alkylating agent C1CH2C029 ®Na in the presence of (C^Hg^NI followed by (CH30)2S02 and the addition of a cosolvent such as dimethyl-15 formamide at from 0°C to 60°C to form ester XXVII XXVII R1-(CEL ) -X1-CH_CO.CH- 2 n 2 2 3 Ester XXVII is then reduced by treating with lithium 20 aluminum hydride, lithium triethylborohydride cr lithium borohydride in the presence of diethyl ether, or tetrahydrofuran to form alcohol XXVIII XXVIII R1-(CH_) -X1-CHoCH-0H 2 n 2 2 which is then converted to the corresponding mesylate by treating XXVIII with mesyl chloride, organic base such as triethylamine in an organic solvent such as methylene chloride. The resulitng 30 mesylate is treated with a sodium halide such as sodium iodide in acetone while heating to a temperature within the range of from about 45 to about 65°C to form the halide XXV.

OA /, 7 e X £ ' "T M J Halide XXVA may be converted to phosphonate IIA or IIC via an Arbuzov reaction wherein XXVA is treated with phosphite XVII XXVA R1-(CH2)n-X1-(CH2)m-Hal (where X1 is 0, S or N-Pro and m=2,3) XVII P(OR2a)3 10 in a molar ratio of XVII:XXVA=20:1 to 3:1 while heating at a temperature within the range of from about 60°C to about 160°C.

Halide XXVA where m=2 and X^=0,S is 15 identical to XXV. Other examples of XXVA are made by treating X with a base, such as NaH in tetrahydrofuran at 0°C to 25°C, followed by reaction with dihalide Hal^-(CH2)m~Hal2, n=2,3, where Hal is CI, Br or I.

The alcohol starting material X where n is 1 and X1 is 0 that is XB XB R1-CH2-0H may be prepared according to the following reaction sequence (following the procedure of E.J. Leopold, Organic Synthesis 1985, 64, pp 164-173) DMSO, (C0C1)2 , (C6H5)3PCH3® I® R1-OH CH2C12, (C2H5)3N C^Li XXI IA Swern Oxidation Wittig Reaction 24 4 3 6 1) BH^, THF Hydroboration ^ XB 2) H202 f NaOH 5 Oxidation The alcohol starting material X where n is 2 and X^" is 0 that is XC XC R1-CH2CH2-OH may be prepared according to the following reaction sequence: 1) CH2(C02alkyl)2, NaH 15 . P(Br)3 malonate alkylation R1-OH > R1-Br >> XXIIA (C2H5)20 XXIX 2) NaCl or LiCl, H20 DMSO, A decarboxylation LiAlH4 (c2h5)2o R-CH-CO,alkyl ^ XC XXX Reduction The alcohol starting material X where n is 3 and X^" is 0, that is XD xd r1-ch2ch2ch2-oh may be prepared according to the following reaction sequence ? 4 4 3 fi { d \u>' 'tw-' CuBr(cat), THF or R^Hal + ClMgOCH2CH2CH2MgCl ^ XD HMPA, THF The amine starting material (X) where X is NH, namely XE R1- (CH0 ) -NH_ 2 n 2 may be prepared according to the following reaction sequence: N-phthalimide alkylation R1-(CH2)n-21 Gabriel Synthesis (Z^ is OTosyl or Hal) when n is 1, 2 or 3 or is Hal when n is 0) XXXI R -(CH2)n-N XXXII nh2nh2 or CH-NHNH, XE Hydrazinolysis The thiol starting material X where X"^ is 30 S, that is XF xf r1-(ch2)nsh 2 4 4 3 6 3 may be prepared according to the following reaction sequence: DIAD OR DEAD, (CX),P 0 1 O 3 J 1 9 II R -(CH2)n-OH ^ R - ( CH )n~ SCCH- 0 ii XG CH3CSH XXXIII "OH XXXIII ^ XF Hydrolysis 1 Examples of starting material X that is r1-(CH2)n-X1H wherein X1 is 0, S, NH or N-Pro and n is 0, 1, 2 or 3 suitable for use herein include the following which are either known in the literature or are simple derivatives of known compounds 20 prepared by employing conventional procedures.

It will be appreciated that the compounds listed in the following table represent all possible stereoisomers. 9 L h u. < * R1-(CH2)n-X1H (where X1 is 0, S, NH, NPro, n is 0, 1, 2 or 3) where R^" is R5-Q^~Q2-Q3- as follows in A. through F. a. r10 ch ch, ch ch, ch \ '/ \ / &\ // \ y ^ n // \ y c ch, c ch, c ch- Mi 2 i R11 CH- CH 3 1. c2h5 ch3 2. CH3 C2H5 3. n"C3H7 CH3 4. CH3 n-C4H9 . • t-C H CH, 6. -(CH2)st-s1=4 to 6 7. h h 8. f f 9. CI CI . ch2f ch3 11. -ch=ch2 h CH- CH CH- . CH /■ 2\ // \ / 2\ \ B. alkyl-(CH,) C CH, C CH, p i * i * ch3 ch3 alkyl(CH2)p- 1. CH3(CH2) where p is 3 to 7 CH-N J 2. C-(CH,)_ where p is 2 to 4 / 2 p CH- 24 4 3 8 ch3 ch3 c. ch3-c=c-ch2kh2-c=c-ch2-»n n=0,1,2,3 ch3 ch3 H-C-CH2-CH2KH2-CH-CH2-CH2*n CHn n=0,1,2,3 d.

R is CH„ CH CH, 's \ s ^ \ s \2 ^ x CH, CH CH.

Ss CH / R11 k6 CH, C «6* CH, 1. c2h5 c2H5 ch3 2. ch3 ch3 c2H5 3. ch3 c2h5 c2h5 4. c2H5 c2h5 c2h5 . ch3 c2h5 ch3 6. ch3 h ch3 7. ch3 ch3 h 8. h h h 244363 39- e.

R1 is ch_ ch C^ ^ ch- ch. ch, N CH R7 CH / V ^ c t ch.

CH„ k6 / CH, r! si s! 1. h i h 2. h h i 3. h ch3 ch3 4. ch3s ch3 h . f ch3 h 6. ch_ 3 ch3 h 7. h ch3 ch3 8. h ch ci 9. h cf3 h . h ci h 11. h ch3 (ch. 12. h ch3 f 2 4 4 3 F. Other examples of r1 include the following ch, ch- ch- ch- ch- ch, \3 / n2 / \2 / n2 / \2 / \2 / ch ch- ch ch- ch ch, I Z | \ £. ch3 ch3 ch3 2. ch, ch0 ch„ ch„ ch„ ch x - un- v.n. un_ v-tt- un ✓ 3 ^ \2 / v2 / .2 / 2 ^ „ / s ch ch- ch ch- c ch, ■ ^ i z | z ch3 ch3 ch3 ch- ^ ch ch, ch- ch- ch . \3 ^ x / \2 / \2 / \2 ^ \ ^ c ch- ch ch, c ch, i 2 , 2 | 2 ch3 ch3 ch3 4. ch, ch . ch, ch ch,-chc-ch' c ch- c ch, i ^ i *■ ch3 ch3 . . ch_ ch, . ch ch, ch / ,2 / x2 // \ / x2 ^ / ch3 ch2 ch ch2 c ch2 ch3 ch, ch, i j i J 6. ch, ch ch ch ch ch v \ ^ x \ \ \ ^ c ch- c ch, c ch, I ^ i ^ i ^ ch3 ch3 ch3 7. ch, „ ch ch- ch ch, ch c ch, c ch, c ch, I 2 i I | ^ ch3 ci ch3 24 4 3 6 3 CH CH.

CH, CH2 CH_ I J C CH, C CH. '/ v CH x- v / CH2 C CH2 I CH. 9.

CH, NT . CH 4 \ S c CH, i l c2H5 CH- CH CH CH ^ \ \ \ / C CH- C CH, i * i ^ CH- CH.

CH3 . CH CH- C CH, V^v2 O ^ y \2 CH- CH- X C CH- C CH ^ \ / CH, i CH. i CH- CH- I 11. CH- C CH- CH CH- CH N3^\ / < ^ \ / ^2 \ C CH- C CH- X C CH, | L- | / I C.

/ CH.

CH.

CH. 12.

CH CH v CH- CH CH, \ / X2 ^ N / 2 CH, C CH, Z | z CH_ CH, i J N ^C\ / CH CH2 CH. 13.

CH, N? CH C c2H5 CH, \ X 2.

CH2 C i CH CH CH / \ CH.

CH & \ CH, CH, 24 4 3 63 CH, 14. CH- V C I CH, CH ch.

CH- ch C I CH, CH- s / v2 CH2 CH CH, f i . CH- CH CH- C CH- CH \3 ^ \ / \ \ C CH- C CH- C CH- i z i z | z CH, CH 3 CH, 16.

CH- < \3 * C CH3 CH.

\ / CH.

\ ^ C l CH- ch CH, \ / CH- \ * C i CH- CH \ / ch2 2 4 4 3 f) ' 3 The compounds of Formula I inhibit cholesterol biosynthesis by inhibition of de novo squalene production. These compounds inhibit the squalene synthetase enzyme and, in addition, some of the compounds of Formula I inhibit other enzymes in the pathway from isopentenyl diphosphate to squalene, that is, farnesyl diphosphate synthetase and isopentenyl diphosphate-dimethylallyl diphosphate isomerase.

Thus, the compounds of Formula I are useful in treating atherosclerosis to inhibit progression of disease and in treating hyperlipidemia to inhibit development of atherosclerosis. In addition, the compounds of the invention may increase plasma high density lipoprotein cholesterol levels.

As squalene synthetase inhibitors, the compounds of Formula I may also be useful in inhibiting formation of gallstones and in treating tumors.

The compounds of Formula I may also be employed in combination with an antihyperlipo-proteinemic agent such as probucol and/or with one or more serum cholesterol lowering agents such as Lopid (gemfibrozil), bile acid sequestrants such as cholestyramine, colestipol, polidexide (DEAE-Sephadex) as well as clofibrate, nicotinic acid and its derivatives, neomycin, p-aminosalicyclic acid, bezafibrate and the like and/or one or more HMG CoA reductase inhibitors such as lovastatin, pravastatin, velostatin or simvanstatin.

The above compounds to be employed in combination with the squalene synthetase inhibitor of the invention will be used in amounts as indicated in the Physicians' Desk Reference (PDR).

Inhibition of squalene synthetase may be measured by the procedure described in New Zealand Patent Specification No. 234502. 2 4 4 3 6J The following Examples represent preferred embodiments of the present invention.

Introduction to Experimental All tempertures are reported in degrees Centigrade. 1 13 H and C chemical shifts are reported as 31 6-values with respect to Me^Si (6=0). P spectra were measured on a JEOL FX9QQ FT-NMR spectrometer, at 31 at 36.2 MHz, utilizing the ^"H decoupled mode. The P data were obtained using 85% H3PC>4 as an external reference (6=0). Coupling constants J are reported in Hz. Chemical ionization mass spectra (CI-MS) were determined with a Finnigan TSQ-4600 instrument equipped with a direct exposure probe using the indicated reagent gases. Fast atom bombardment mass spectra (FAB-MS) were recorded on a VG Analytical ZAB-2F spectrometer. Ions were sputtered (8keV Xe) from a matrix containing dithiothreitol, dithioerythritol, DMSO, glycerol and water.

All reactions were carried out under an atmosphere of dry argon or nitrogen. The following reagents and solvents were distilled prior to use from the indicated drying agents, where applicable: CE^Cl.^, 2 , 4, 6-collidine, and diisopropylamine (CaH^); THF and diethyl ether (K, benzophenone); N,N-diethyltrimethylsilylamine and oxalyl chloride. Benzene was passed through 24*3 • s \y neutral alumina (activity I) and stored over 4A-molecular sieves. Lithium bromide was dried at 100°C over P205.(E,E)-Farnesol was purchased from Aldrich Chemical Company.

TLC was performed on E. Merck Silica Gel 60 F-254 plates (0.25 mm) or E. Merck Cellulose F plates (0.1 mm). Flash chromatography was carried out using E. Merck Kieselgel 60 (230-400 mesh).

Reverse-phase chromatographic purification 10 of PMP salts was carried on CHP20P gel (75-150 ^), a highly porous, polystyrene-divinyl benzene copolymer available from Mitsubishi Chemical Industries. The indicated general procedure was followed: An FMI Model RP-SY pump was utilized 15 for solvent delivery. A column of CHP20P (2.5 cm diameter, 12-22 cm height) was slurry packed and washed with water (500-1000 mL), and a basic, aqueous solution of the crude salt was applied to the top of the column. Typically, the column was 20 eluted with water, followed by a gradient composed of increasing concentrations of acetonitrile or methanol in water. The gradient was created by placing the tip of a tightly stoppered separatory funnel containing 300-500 mL of the organic 25 solvent, or an aqueous-organic mixture, just beneath the surface of a reservoir containing 300-500 mL of pure water. To start the gradient, the stopcock of the separatory funnel was opened, so that as the solvent was withdrawn by the pump 30 from the reservoir, it was replaced with the solvent from the separatory funnel. HPLC-grade solvents and Lectrostill steam distilled water 24 -4 6- were employed. Fractions were collected (10-15 mL each) at a flow rate of 5-10 mL per minute. Those fractions that contained pure product as judged by TLC were pooled, the organic solvents 5 were evaporated and the aqueous residue was lyophilized to dryness. 24 4 J Example 1 (E,E)-[[(1-Methylethoxy)[[(3,7,ll-trimethyl-2,6,10-dodecatrienyl)oxy]methyl]phosphinyl]methyl]phosphonic acid, dimethyl ester A. (Hydroxymethyl)phosphonic acid, bis(1-methylethvl) ester A mixture of 33.2 g (0.20 mol) of diisopropyl phosphite, 2.8 ml (0.02 mol) of triethylamine, and 6.0 g (0.20 mol) of 10 paraformaldehyde, was immersed in a 100°C oil bath and then heated between 100-120°C for 45 minutes under nitrogen. An exotherm occurred within 10 minutes and all of the paraformaldehyde dissolved rapidly. The triethylamine was removed at reduced 15 pressure, and the residue was bulb-to-bulb distilled in four portions to provide a total of 35.17 g (91%) of title compound as a colorless oil. TLC Silica gel (5:95 CH3OH:CH2Cl2) Rf=0.17 XH NMR (CDC13, 270 MHz) 64.73 (sextet, 3H, J=6 Hz) 3.84 (d, 2H, J=6 Hz) 1.34 (d, 12H, J=6 Hz) ppm. 13C NMR (CDC13, 67.8 MHz) 570.9 (d, J=6 Hz) 57.5 (d, J=162 Hz) 23.8 (d, J=6 Hz) ppm.

B. [[[(Trifluoromethyl)sulfonyl]oxy]-methyl]phosphonic acid, bis(1-methyl- ethyl) ester To a stirred solution of 6.0 g (30.6 mmol) of Part A phosphonate in 100 ml of dry diethyl ether (also referred to as ether) at -78°C was added 5.90 24 4 3 63 ml (33.9 mmol) of diisopropylethylamine followed by the addition of 5.20 ml (31.0 mmol) of trifluoromethanesulfonic anhydride in 10 ml of ether over 30 minutes. An additional 40 ml of ethyl ether was 5 added to aid stirring through the thick precipitate. After 45 minutes at -78°C, the reaction was allowed to warm to 0°C for 45 minutes, and the solids were filtered and washed with ether. The filtrate was evaporated to afford 9.4 g of a colorless liquid.

The crude product was flash chromatographed on 150 g of silica gel eluted with 40:60 ethyl acetate:hexane to provide 5.7 g (57%) of pure title triflate as a colorless liquid.

TLC Silica gel (50:50 Ethyl Acetate:Hexane) R^=0.34.

NMR (CDC13, 270 MHz) 54.79 (m, 2H) 4.55 (d, 2H, J=8.8 Hz) 1.37, 1.39 (two d, J=6 Hz) ppm. 13C NMR (CDC13, 67.8 MHz) 518.5 (q, J=319 Hz) 73.0 (d, J=7.8, Hz) 67.1 (d, J=170 Hz) 23.8, 23.7 (two d, J=10 Hz) ppm.

C. (E,E)-[[(3,7,ll-Trimethyl-2,6,10-dodeca-trieny1)oxy]methyl]phosphonic acid, bis(1-methylethyl) ester 1) Preferred Method A solution of potassium hexamethyldisilazide (1.4 M in tetrahydrofuran (THF), 14.3 mL, 20 mmol) was added dropwise over 5 minutes to a solution of (hydroxymethyl)phosphonic acid, bis(1-methylethyl) 24 4 3 ester (3.92 g, 20 mmol) in THF (40 mL) at ice bath temperature under argon. A precipitate formed in 3 minutes. After 10 minutes a solution of (E,E)-farnesyl chloride (4.82 g, 20 mmol) in dry THF 5 (10 mL) was added dropwise over 4 minutes and the mixture was stirred for 2.5 hours at 0° and for 3 hours at room temperature. The reaction was quenched with acetic acid (1.2 g, 20 mmol); the color changed from orange to pale yellow. The 10 mixture was poured into ethyl acetate (EtOAc) (50 mL) and washed twice with 30 mL 50% brine and 30 mL brine. The organic layer was dried (MgSO^) and solvent was evaporated to give 8.1 g (100%) of crude title product. The product was purified 15 by flash column chromatography over silica gel (200 g). The column was prepared in hexane, the material was charged neat and eluted successively with 100 mL hexane, 4 L 25% EtOAc/hexane and 1 L 50% EtOAc/hexane. Fractions 53 to 108 (45 mL 20 each) were combined and evaporated to give 5.6 g (yield 70%) of title product.

TLC Silica gel (50:50 Ethyl Acetate:Hexane) R^=0.26. IR (CC14) 2978, 2929, 1450, 1384, 1374, 1256, 1240, 1107, 990 cm"1. 1H NMR (CDC13, 270 MHz) 65.32 (t, 1H, J=7 Hz) .09 (m, 2H) 4.76 (m, 2H) 4.12 (d, 2H, J=7 Hz) 3.68 (d, 2H, J=8.8 Hz) 2.06 (m, 8H) 1.68 (s, 6H) 1.60 (s, 6H) 1.34, 1.33 (two d, 12 H, J=6 Hz) ppm. 24 4 3 6 3 Mass Spec (Cl-CH4, + ions) m/e 401 (M+H), 197.

Anal. Calcd. for C22H4^04P: C, 65.97; H, 10.32; P, 7.73 Found: C, 66.03; H, 10.32; P, 7.67 2) Alternate Preferred Method (E,E)-Farnesol was purified further by flash chromatography on silica gel eluted with 5:95 ethyl acetate:hexane.

To a stirred solution of 2.0 g (9.0 mmol) 10 of purified (E,E)-farnesol in 22 ml of tetrahydrofuran (THF) under argon at -78°C was added 5.4 ml (8.61 mmol) of 1.6 M n-butyllithium in hexanes over 15 minutes. The reaction was allowed to stir for 40 minutes at -78°C, when 2.69 15 g (8.19 mmol) of the Part B triflate in 7 ml of THF was added via cannula. After 30 minutes at -78°C, the reaction was allowed to warm to 0°C for two hours. The reaction was quenched with saturated ammonium chloride and partitioned 20 between ethyl ether and water. The ether layer was washed with brine, dried (MgS04) and evaporated to provide 3.5 g of a pale yellow oil. The crude product was purified by flash chromatography on 350 g of silica gel packed in 25 20:80 and eluted with 30:70 ethyl acetate:hexane to provide 3.04 g (92%) of title ether as a colorless liquid.

D. (E,E)-[[(3,7,ll-Trimethyl-2,6,10-dodeca-30 trienyl)oxy]methyl]phosphonic acid, mono(1-methylethyl) ester To a solution of 1.57 g (3.91 mmol) of Part C ether in 20 ml of 2-propanol under argon was added 20 ml of 1 N KOH, and the reaction was heated to 24 4 3 6 3 105°C for 48 hours. After cooling to room temperature, the 2-propanol was evaporated and the aqueous residue was stirred with dichloromethane and acidified with 10% HCl. The organic layer was 5 washed with water and brine, dried (MgSO^), and evaporated to provide 1.39 g (96%, corrected for 0.37 mol equiv of dichloromethane) of title compound as a colorless oil.

TLC Silica gel (8:1:1 1-propanol:con NH_:H_0) 10 Rf=0.55 . 1H NMR (CDC13, 270 MHz) 55.28 (t, 1H, J=7 Hz) .09 (m, 2H) 4.72 (m, 2H) 4.12 (d, 2H, J=7 Hz) 3.70 (d, 2H, J=7 Hz) 2.06 (m, 8H) 1.67 (s, 6H) 1.60 (s, 6H) 1.33 (d, 6H, J=6 HZ) ppm.

E. (E,E)-[[(1-Methylethoxy)[[(3,7,11-trimethyl-2,6,10-dodecatrienyl)oxy]-methyl]phosphinyl]methyl]phosphonic acid, dimethyl ester To a stirred solution of 1.395 g (3.89 mmol) of Part D compound in 8 ml of dichloromethane under argon was added 1.5 ml (7.51 mmol) of distilled N,N-diethyl(trimethylsilyl)amine. 30 The reaction was allowed to stir for 1.5 hours at room temperature, the solvent was evaporated and the residue was dissolved in benzene, evaporated 2 4 4 3 6 3 and then pumped at high vacuum. The remainder was dissolved in 8 ml of dichloromethane containing three drops of dimethylformamide (DMF) under argon at 0°C, and 0.63 ml (7.8 mmol) of distilled 5 oxalyl chloride was added dropwise over 10 minutes, with much gas evolution. After 45 minutes at 0°C, the reaction was allowed to warm to room temperature for 45 minutes. The solution was evaporated and the residue was twice dissolved 10 in benzene and evaporated, followed by pumping at high vacuum.

To a solution of 0.93 ml (8.58 mmol) of dimethyl methylphosphonate in 22 mi of THF at -78°C under argon was added 5.2 ml (8.36 mmol) of 15 n-butyllithium in hexane over 5 minutes to give a white suspension. After 40 minutes, the acid chloride prepared above was added in 8 ml of THF over 10 minutes. The reaction was allowed to stir for one hour at -78°C, when it was quenched with 20 saturated ammonium chloride and diluted with ethyl ether. The aqueous layer was made acidic with 10% KC1 and the organic layer was separated and washed with brine. The aqueous layer was re-extracted with dichloromethane, and the dichloromethane 25 layer was washed with brine. The combined organic layers were dried (MgSO^) and evaporated to provide 1.84 g of a crude yellow oil. Flash chromatography on 200 g of silica gel eluted with 2:98 methanol:dichloromethane gave 1.49 g (82%) of 30 pure title triester as a colorless oil.

TLC Silica gel (5:95 CH3OE:CH2Cl2) Rf=0.21 2 4 4 3 8 3 IR (CC14) 2977, 2954, 2926, 2853, 1449, 1385, .1375, 1256, 1229, 1063, 1036, 992, 841 cm"1. XH NMR (CDC13, 270 MHz) 65.32 (t, 2H, J=7 Hz) 5.09 (m, 2H) 4.78 (m, 2H) 4.10 (d, 2H, J=7 Hz) 3.79, 3.83 (two d, 6H, J=6 Hz) 3.6-3.9 (m, 2H) 2.50 (m, 2H) 2.07 (m, 8H) 1.68 (s, 6H) 1.60 (s, 6H) 1.34, 1.37 (two d, 6H, J=7 Hz) ppm. 15 Mass Spec (CI-CH4, + ions) m/e 505 (M+C-jH^), 493 (M+C2H5), 465 (M+H).

Example 2 (E,E)-[[Hydroxy[[(3,7,ll-trimethyl-2,6,10-dodeca-20 trienyl)oxyjmethyl]phosphinyl]methyl]phosphonic acid, tripotassium salt To a stirred solution of 654 mg (1.42 mmol) of Example 1 triester in 7 ml of dry dichloromethane at room temperature was added 0.47 25 ml (3.54 mmol) of 2,4,6-collidine followed by 0.94 ml (7.09 mmol) of bromotrimethylsilane. The reaction was allowed to stir for 23 hours at room temperature, the solution was evaporated, the residue was dissolved in benzene, evaporated, and 30 pumped at high vacuum. The remainder was dissolved in 8 ml of 1 M KOH, stirred for 30 minutes, diluted with water and lyophilized. The crude material was purified by MPLC on a column ? 4 L 3 •—j i vj? of CHP20P (2.5 cm diameter x 20 cm height) eluted with water (fractions 1-12), followed by a gradient created by the gradual addition of acetonitrile (500 ml) to a reservoir of 400 ml of 5 water. Approximately 15 ml fractions were collected. Fractions 27 - 33 were combined, the acetonitrile was evaporated at reduced pressure, and the aqueous solution was lyophilizea to provide 680 mg (93%) of title product in the form 10 of a dense, amorphous white Ivophilate. Further drying under vacuum led to an insignificant loss of mass. The pH of a 1% w/v solution was 8.9. TLC Silica gel (5:4:1 1-propanol:con NH^-.H^O) R^=0.44. IR (KBr) 3400 (broad), 2967, 2921, 2860, 1662, 15 1445, 1381, 1180, 1146, 1085, 1054, 967, 867, 789, 466 cm 1H NMR (D20, 270 MHz) 65.34 (t, 1H, J=7 Hz) .07, 5.08 (two t, 2H, J=7 Hz) 20 4.07 (d, 2H, J=7 Hz) 3.57 (d, 2H, J=6.4 Hz) 1.8-2.2 (m, 10H) 1.64 (s, 3H) 1.60 (s, 3H) 1.54 (s, 6H) ppm. 13 C NMR (67.8 MHz, D20] 6144.35, 137.63, 134.48, 125.46, 125.30, 120.50, 70.17 (d, J=ll.36 Hz), 69.30 (d, J=113.55 Hz), 39.9, 39.8, 30.45 (dd, J=119.23 Hz, 79.48 Hz) 26.78, 26.64, 30 25.89, 17.99, 16.74, 16.29 ppm. 24 4 3 8 3 31P NMR (D20, 36.2 MHz) 532.1 (d, J=9.6 Hz) 12.7 (d, J=9.6 Hz) ppm.

Mass Spec (FAB, + ions) m/e 509 (M+H).

Analysis Calcd for C17H2gK3P206 + 0.37 mol H20 (Effective MW = 515.3): C, 39.61; H, 5.83; P, 12.02 Found: C, 39.98; H, 5.99; P, 12.29 Example 3 (E,E)-[[Methoxy[[(3,7,ll-trimethyl-2,6,10-dodeca-trienyl ^xy]methyl ] phosphinyl ]methyl ]phosphonic acid, dimethyl ester A. (E,E)-[[(3,7,ll-Trimethyl-2,6,10-dodeca-trienyl)oxy]methyljphosphonic acid, dimethyl ester (E,E)-Farnesol, (5.0 mmol, 1.11 g, 1.26 ml) was stirred under argon in 10 ml of dry deoxygenated benzene (10 ml) and treated in one 0 |i portion with Rh2(0CCH3)4 (1.0 mmol, 0.442 g). The dark blue/green solution was stirred at room temperature and a solution of (1.5 g, 10.0 mmol) of dimethyl diazomethylphos-phonate (prepared according to D. Seyferth et 25 al, J. Org. Chem., 1971, vol. 36, No. 10, pages 1379 to 1386) in 20 ml of dry, deoxygenated benzene was added dropwise over 4 hours via syringe pump. After addition, the reaction mixture was stirred an additional 45 minutes. The reaction mixture was 30 stripped of solvent and applied as a 75% ethyl acetate/Hexane solution to a flash chromatography column (50 mm diameter, 6" of silica gel) eluted with 75% ethyl acetate/hexane to afford 0.627 g, (36% yield), of the ether as a dark blue/green 35 oil. 24 4 3 6 — 56- TLC: Silica gel (EtOAc) Rf = 0.32 IR (film) 2960, 2920, 2855, 1720, 1665, 1595, 1445, 1380, 1260, 1185, 1110, 1070-1025 (br), 890, 835, 805, cm""1.

Mass Spec (CI, + ions) m/e 345 (M+H) 1H NMR (270 MHZ, CDC13) 6 5.32 (t, 1H, J=7 Hz) .09 (m, 2H) 4.13 (d, 2H, J=7 Hz) 3.83 (d, 6H, J=ll Hz) 3.79 (d, 2H, J=ll Hz) 2.13-1.93 (m, 8H) 1.71 (s, 6H) 1.62 (s, 6H) ppm. 13C NMR (67.8 MHz, CDC13) 142.03, 135.28, 131.13, 124.18, 123.54, 119.52, 69.26, (d, J=13.24 Hz), 62.44 (d, J=166.53 Hz), 52.82 (d, J=7.57 Hz), 39.53 (d, J=3.78 Hz), 26.61, 26.17, 25.53, 17.52, 16.37, 15.87 ppm.

B. (E,E)-[ [(3,7,ll-Trimethyl-2,6,10-dodecatrienyl )oxy]methyljphosphonic acid, monomethyl ester The Part A dimethylphosphonate (1.73 mmol, 25 0.595 g) was stirred under argon in 8.65 ml of methanol and treated with a solution of KOH (34.55 mmol, 1.93 g) in 8.65 ml of H20. The pale yellow reaction mixture was heated to reflux for 8 hours, cooled to room temperature, and the methanol 30 removed in vacuo. The resulting residue was diluted with 30 ml H20 and treated portionwise with 5% HC1 (aqueous) until the pH=l. This aqueous phase was extracted with ethyl acetate. 2 4 k 5 fi The organic extracts were combined, dried over MgSO^, azeotroped with benzene, and stripped in vacuo to afford (0.483 g, 84% yield) of the title phosphonate monoester as a brown oil.

TLC silica gel (20:1:1 CH2C12:CH3OH:CH3COOH) Rf=0.05 IR (film) 2960, 2920, 2860, 1730, 1710, 1705, 1695, 1680, 1665, 1445, 1380, 1245 - 1175 (broad), 1110, 1050, 995, 880, 830, 740, 680, cm"1.

Mass Spec (CI, -ions) m/e 329 (M-H) XH NMR (270 MHz, CDCl3) 65.32 (t, 1H, J=6.9 Hz) .08 (m, 2H) 4.13 (d, 2H, J=6.9 Hz) 3.81 (d, 3H, J=10.2 Hz) 3.76 (d, 2H, J=10.2 Hz) 2.21-1.92 (m, 8H) 1.71 (s, 6H) 1.61 (s, 6H) ppm. 13 C NMR (67.8 MHZ, CDC13) 6141.98, 135.39, 131.24, 124.29, 123.68, 119.69, 69.37, (d, J=13.24 Hz), 63.22 (d, J=170.32 Hz) 52.58 (d, J=7.57 Hz), 39.64, 26.70, 26.31, 25.64, 17.63, 16.49, 15.98 ppm.

C. (E,E)-[[Methoxy[[(3,7,11-trimethyl- 2,6,10-dodecatrienyi)oxy]methyl]phos-phinyljmethyljphosphonic acid, dimethyl ester The Part B phosphonate monoester (1.39 30 mmol, 0.460 g) was stirred under argon in 12.5 ml of dry CH2C12 and treated dropwise with diethyl trimethylsilylamine (2.78 mmol, 0.405 g, 0.528 ml, freshly distilled). This reaction mixture was 2 L A ? £ ? t- i r ^ •._/ stirred at room temperature for 2 hours. Then, the solvent was evaporated and the resulting residue azeotroped two times with 40 ml of benzene and pumped under high vacuum for 30 minutes. The 5 residue was then stirred under argon in 12.5 ml of dry CH2C12, cooled to 0°C and treated with 2 drops of dry dimethylformamide (DMF), followed by dropwise addition of oxalyl chloride (2.50 mmol, 0.318 g, 0.218 ml). This reaction was stirred at 0°C for 1 10 hour and then warmed to room temperature and stirred for 45 minutes. Then, the solvent was removed and the residue azeotroped and pumped under high vacuum as above. Finally, the phosphonochloridate was stirred under argon in 2 ml of dry 15 THF and cooled to -78°C and added dropwise (10 minutes) to a -78°C solution of the lithium anion of dimethylmethylphosphonate.

The anion of dimethylmethylphosphonate was formed by stirring dimethylmethylphosphonate (3.18 20 mmol, 0.395 g, 0.345 ml) under argon in 8 ml of dry THF cooling to -78°C, treating this solution dropwise with n-butyllithium (3.05 mmol, 1.22 ml of a 2.5 M solution in hexane) and then stirring at -78°C for 30 minutes. The cooled solution of the 25 phosphonochloridate (see above) was added dropwise to the -78°C solution of the dimethylmethylphosphonate anion and then the reaction was stirred at -78°C for 1 hour, warmed to 0°C and stirred an additional 45 minutes. The reaction mixture was quenched with 30 20 ml saturated aqueous NH^Cl warmed to room temperature, diluted with 20 ml H20, and the aqueous layer extracted 2 times with ethyl acetate and 2 times with ethyl ether. The organic extracts 2 4 4 3 6 3 were combined, dried over MgSC>4, filtered and the solvent removed in vacuo. The product was isolated via flash chromatography (50 mm diameter column, 6" of silica gel, 4% CH30H/CH2C12 eluent) to afford 5 0.273 g, (45% yield) of the title triester as an orange oil.

TLC Silica gel (5% CH3OH/CH2Cl2) Rf=0.29 IR (film) 2960, 2920, 2858, 1710, 1663, 1448, 1380, 1310, 1250, 1185, 1070 - 1020, 920, 888, 10 842, 820 cm"1.

Mass Spec (CI, + ions) m/e 437 (M+H).

XH NMR (270 MHz, CDCl3 ) 65.32 (t, 1H) .09 (m, 2H) 4.12 (d, 2H, J=6.9 Hz) 3.83 (d, 3H, J=ll Hz) 3.83 (d, 2H, J=7.9 Hz) 3.81 (d, 6H, J=li Hz) 2.66-2.44 (m, 2H) 2.16-1.96 (m, 8H) 1.69 (s, 6H) 1.60 (s, 6H) ppm. 13C NMR (67.8 MHz, CDCl3) 6 142.03, 135.25, 131.07, 124.12, 123.48, 119.41, 25 69.45, (d, J=13.25 Hz), 64.77 (d, J=119.23 Hz), 52.96 (d, J=5.68 Hz), 52.77 (d, J=5.68 Hz), 51.74 {d, J=5.68 Hz), 39.47, 26.56, 26.17, 24.02 (dd, J=136.25 Hz, 83.27 Hz), 25.50, 17.49, 16.37, 15.82 ppm. 2 4 4 3 n 3 Example 4 (E,E)-[[Hydroxy[[(3,7, ll-trimethyl-2,6,10-dodeca-trienyl)oxy]methyl]phosphinyl]methyl]phosphonic acid, tripotassium salt The Example 3 triester (0.459 mmol, 0.20 g) was stirred under argon in 3.0 ml of dry CHjC^. This solution was cooled to 0°C and treated with dry 2,4,6-collidine (0.918 mmol, 0.111 g, 0.121 ml) followed by dropwise addition of 10 bromotrimethylsilane (1.84 mmol, 0.281 g, 0.242 ml). The reaction mixture was stirred at 0°C for 1 hour, warmed to room temperature, and stirred an additional 2 hours. The solvent was evaporated in vacuo and the residue dried under high vacuum 15 for 40 minutes. The resulting yellow solid was dissolved in 1.8 ml of methanol. This solution was cooled to 0°C and treated with 1.8 ml of IN KOH. The solution was stirred at room temperature for 30 minutes. The solvents were removed 20 in vacuo and the residue pumped under high vacuum for 3 hours. The product was purified via CHP20P chromatography. The reaction product in 3 ml of distilled H^O was applied to a CHP20P column (2.5 cm diameter, 17 cm height). The column was eluted 25 with 250 mL H20, followed by an acetonitrile/water gradient. Fractions were collected every 1.4 minutes (^ 10 ml). Product fractions were combined, evaporated, lyophilized (16 hours), and dried under high vacuum over ^or ® hours to afford 0.113 g (48% yield) of title salt as a slightly off-white, hygroscopic lyophilate.

For spectroscopic characterization, see Example 2. 2 4 4 3 $ 3 Anal. Calcd. for C17H3Q06P2K2* 0.5 moles HjO C, 42.57; H, 6.30 Found: C, 42.70; H, 6.64 Example 5 (E)-[[[[(3,7-Dimethyl-2,6-octadienyl)oxy]methyl]-ethoxyphosphinyl]methyl]phosphonic acid, dimethyl ester A. (Hydroxymethvl)pnosphonic acid, diethyl ester The procedure of Kluge was followed (Org Syn 1986, Vol. 64, 80-84). The following ingredients were combined: 69 g (0.50 mol) of diethyl phosphite, 15 g (0.50 mol) of paraformaldehyde, and 5.1 g 15 (0.05 mol) of triethylamine. The stirred mixture was immersed in a preheated oil bath at 120°C and heated for one hour. Upon cooling, the triethylamine was removed on the rotary evaporator at 80°C, and the residue was then Kugelrohr distilled (150-160°C, 20 0.10 mm) to provide 57.5 g (68%) of title compound as a colorless liquid.

TLC: silica gel (5:95 CH3OH:CH2C12) Rf=0.19 1H NMR (CDC13) (270 MHz) 54.95 (br, 1H) 4.09 (quint, 4H, J=7 Hz Hz) 3.83 (d, 2H, J=5.8, Hz) 1.27 (t, 6H, J=7 Hz) ppm. 13C NMR (CDC13) (67.8 MHz) 662.2 (d, J=7.6 Hz) 56.5 (d, J=162.8 Hz) 16.1 (d, J=5.7 Hz) ppm. 2 4 4 3 6 5 B. [[[(Trifluoromethyl)sulfonyl]oxy]-methyl1phosphonic acid, diethyl ester A modification of the literature procedure was developed (Phillipon, D.P.; Andrew, S.S.

Tetrahedron Letters 1986, 27, 1477-80). To a solution of 5.00 g (30.0 mmol) of Part A phosphonate and 5.2 ml (30.0 mmol) of diisopropylethylamine in 100 ml of dry ethyl ether at -78°C under nitrogen was added a solution of 5.05 ml (30.0 mmol) of 10 trifluoromethanesulfonic anhydride in 10 ml of ether over 15 minutes. After one hour at -78°C, the reaction was allowed to warm gradually to 10°C over 75 minutes. The precipitated amine salt was removed by filtration and the filtrate was 15 concentrated to provide 5.94 g of crude triflate as a yellow oil. Flash chromatography on 100 g of silica gel eluted with 50:50 ethyl acetate:petroleum ether provided 4.31 g (48%) of title triflate as a clear, colorless liquid.

TLC Silica gel (50:50 ethyl acetate:hexane) R^=0.30.

XH NMR (CDC13) (270 MHz) 64.63 (d, 2H, J=9 Hz) 4.25 (quint, 4H, J=7 Hz) 1.39 (t, 6H, J=7 Hz) ppm. 13C NMR (CDC13) (67.8 MHz) 6118.4 (q, J=319.8 Hz) 66.3 (d, J=168.5 Hz) 63.8 (d, J=7 .6 Hz) 16.1 (d, J=5.7 Hz) ppm. 24 4 3 C• (E)-[[(3,7-Dimethyl-2,6-octadienyl)oxy]-methyl 1phosphonic acid, diethyl ester To a stirred solution of 830 mg (5.38 mmol) of (E)-geraniol (Aldrich Chemical) in 12 ml of 5 tetrahydrofuran at -78°C under argon was added 3.5 ml (5.65 mmol) of 1.6 M n-butyllithium in hexane over five minutes. After 30 minutes, 1.78 g (5.92 mmol) of Part B triflate in 6 ml of tetrahydrofuran was added over five minutes. After 30 minutes at 10 -78°C, the solution was allowed to warm to -20°C for one hour. The reaction was diluted with ethyl ether, washed with saturated NH^Cl, water and brine, dried (MgS04), and evaporated to provide 1.65 g of a colorless oil. Flash chromatography on 15 200 g of silica gel eluted with 43:57 ethyl acetate:hexane gave 1.21 g (74%) of title diester containing a trace impurity. An additional chromatography on 150 g of silica eluted with 30:70 ethyl acetate: hexane gave pure title diester as a 20 colorless oil.

TLC Silica gel (75:25 ethyl acetate:hexane) R^=0.29. IR (CC14) 2981, 2929, 2912, 1443, 1391, 1259, 1098, 1055, 1031, 967 cm"1. 1H NMR (CDC13) (270 MHz) 55.32 (t, 1H, J=7 Hz) .08 (br, 1H) 4.18 (m, 6H) 3.74 (d, 2H) 2.08 (m, 4H) 1.68 (s, 6H) 1.60 (s, 3H) 1.35 (t, 3H, 3-1 Hz) ppm.

Mass Spec (CI-CH4/N20( + ions) m/e 609 (2M+H), 473, 345 (M+C3H5), 333 (M+CgHg), 305 (M+H).

D. (E)-[[(3,7-Dimethyl-2,6-octadienyl)-5 oxy]methyl]phosphonic acid, monoethyl ester A solution of 1.00 g (3.28 mmol) of Part C diester in 25 ml of ethanol was treated with 17 ml of 1M KOH under nitrogen and the solution was 10 heated to 75°C for 2.75 hours. The pH was adjusted to 7 with 10% HCl, and the ethanol was evaporated. The aqueous residue was stirred with 100 ml of dichloromethane and acidified with 10% HCl. The aqueous layer was extracted with an 15 additional 50 ml of dichloromethane and the combined organic layers were washed with 20 ml of 1:1 brine:water, dried (MgS04) and evaporated to provide 911 mg (100%) of title compound as a pale yellow oil.

TLC silica gel (7:2:1 n-C3H7OH-.con NH3:H20) Rf=0.59 1H NMR (CDC13) (270 MHz) 65.32 (t, 1H, J=7 Hz) .08 (br t, 1H) 4.15 (m, 4H) 3.74 (d, 2H, J=9 Hz) 2.06 (m, 4H) 1.67 (s, 6H) 1.60 (s, 3H) 1.35 (t, 3H, J=7 Hz) ppm. 2 4 4 3 6 E- [[[[(3,7-Dimethyl-2,6-octadienyl)oxy]-methyl ] ethoxyphosphiny 1 ] methyl ] phosphonic acid, dimethyl ester A solution of 903 mg (3.27 mmol) of Part D 5 compound in 8 ml of dichloromethane under argon was treated with 1.25 ml (6.54 mmol) of diethyl(trimethylsilyl)amine. After 100 minutes, the solution was evaporated, and the residue was evaporated with benzene and pumped at high 10 vacuum. To the silyl ester in 8 ml of dichloromethane contaning two drops of dimethylformamide at 0°C under nitrogen was added 0.57 ml (6.54 mmol) of oxalyl chloride, dropwise over 15 minutes. After 20 minutes, the reaction 15 was allowed to warm to room temperature for one hour. The volatiles were evaporated and the acid chloride residue was evaporated with benzene and pumped at high vacuum.

To a solution of 0.78 ml (7.19 mmol) of 20 dimethyl methylphosphonate in 15 ml of tetrahydrofuran at -78°C under argon was added 4.4 ml (7.03 mmol) of 1.6 M n-butyllithium over 10 minutes, resulting in a white precipitate. After 30 minutes, a solution of the acid chloride 25 described above in 5 mi of tetrahydrofuran was added dropwise over 5 minutes. After 70 minutes at -78°C, the reaction was quenched with saturated NH^Cl, diluted with dichloromethane (100 ml) and the aqueous layer acidified with 1M HCl. The 30 aqueous layer was separated and re-extracted with 100 ml of dichloromethane. The combined organic layers were dried (MgSO^) and evaporated to provide 1.65 g of crude product. After pumping 2 4 4 3 6 under high vacuum overnight to remove excess dimethyl methylphosphonate, the crude product was chromatographed to provide 1.04 g (80%, corrected for 0.2 equiv of CH2Ci2 observed by *H NMR) of 5 pure title compound as a colorless oil.

TLC Silica gel (5:95 CH30H:CH2C12) Rf=0.19 IR(CC14) 2955, 2928, 2855, 1455, 1257, 1230, 1063, 1037, 841 cm-1. 1H NMR (CDC13), (270 MHz) 65.32 (td, 1H, J=7, 1Hz) .09 (br t, 1H) 4.19 (m, 2H) 4.14 (a, 2H, J=6.8 Hz) 3.83 (d, 3H, J=ll.6 Hz) 3.80 (d, 3H, J=ll Hz) 2.52 (m, 2H) 2.07 (m, 4H) 1.68 (s, 5H) 1.60 (s, 3H) 1.36 (t, 3H, J=7 Hz) ppm.

Mass Spec <CI-CH4/N20' + ions) m/e 383 (M+H), 351 (M+H-CH3OH), 247.

Example 6 (E)-[ [ [ [ (3,7-Dimethyl-2, 6-octadienyl )oxy]methyl]- hydroxyphosphinyl]methyl]phosphonic acid, trisodium salt To a solution of 536.3 mg (1.40 mmol) of Example 5 triester in 6 ml of dichloromethane 30 under argon was added 0.42 ml (3.16 mmol) of 2,4,6-collidine followed by 0.83 ml (6.30 mmol) of bromotrimethylsilane. The reaction was allowed to stir for 3.5 hours at room temperature, and was 2^4 * & c « t \J u then evaporated and pumped at high vacuum. The residue was dissolved in 4.5 ml (4.5 mmol) of 1 M NaOH and then adjusted to pH 12 with several additional drops of 1 M NaOH. This solution was lyophilized and the residue was purified by MPLC on a 2.5 cm diameter, 22 cm height column of CHP20P packed and eluted with water to provide, after freeze-drying and further drying at high vacuum, 496 mg (90%) of title compound as a white lyophilate. TLC Silica gel (4:4:1 n-C3H7OH:con NH3:H20) Rf=0.46 IR (KBr) 3435, 2967, 2923, 2857, 1636, 1175, 1153, 1088, 1056, 974, 797 cm"1.

XH NMR (D20 (400 MHz) 65.35 (t, 1H, J=7 Hz) 5.14 (t, 1H, J=7 Hz) 4.08 (d, 2H, J=7 Hz) 3.60 (d, 2H, J=6.2 Hz) 2.0-2.1 (m, 4H) 1.92 (t, 2H, J=18 Hz) 1.62; 1.64 (two S, 6H) 1.56 (s, 3H) ppm. 31P NMR (D20) (36.2 MHz) 635.3 (d, J=8.8 Hz) .1 (d, J=8.8 Hz) ppm.

Mass Spec (FAB, + ions) m/e 415 (M+Na), 393 (M+H) Anal Calcd for CnP_OcNa_•1.03 H_0: Iz Z± Z D J Z C, 35.09; H, 5.66; P, 15.08 Found: C, 35.49; H, 5.97, P, 14.74 24 4 <$ o - 68-Example 7 (E,E)-[[Ethoxy[[(4,8,12-trimethyl-3,7,11-trideca-trienyl)oxy]methyl]phosphinyl]methyl]phosphonic acid, dimethyl ester A. (E,E)-4,8,12-Trimethyl-3, 7,11-trideca- trien-l-ol (1) (E,E)-3,7,ll-Trimethyl-2, 6,10-doaeca- trienaldehvde f(E,E)-Farnesal 1 A solution of oxalyl chloride (4.68 g, 0.037 mol) in dry C^Cl, under argon atmosphere was cooled to -65°C. A solution of dimethyl sulfoxide (DMSO) (5.33 ml) in CH2C12 (17 mi) was added rapidly, dropwise, to the cooled oxalyl chloride solution. After stirring for 7 minutes at 15 -65°C, a 10 ml CH2C12 solution of (E,E)-farnesol (7.0 g, 0.032 mol) was added over 10 minutes to the reaction solution at -65°C: a precipitate formed upon the addition of approximately half of the farnesol solution. After the addition of the 20 farnesol solution was completed, the reaction was stirred at -65°C for 25 minutes, and then 22.4 ml (0.16 mol) of triethylamine was added over 10 minutes. After stirring for an additional 15 minutes at -65°C, the reaction was warmed to room 25 temperature, and then diluted with water (^200 ml). The resulting aqueous layer was extracted several times with CH2C12. The combined organic layers were washed once with saturated aqueous NaCl solution once with 1% HCl, once with 5% Na2C03 30 solution and once with saturated aqueous NaCl solution. The resulting organic layer was dried over MgSO^ to give 7.05 g (100%) of a clear oil after filtration and solvent removal. 24436 TLC Silica gel (20% ethyl acetate/hexane) Rf=0.34. 1H NMR (CDC13) (270 MHz) 69.98 (d, 1H, J=7 Hz) .88 (broad d, 1H, J=7 Hz) 5.08 (m, 2H) 2.22 (m, 4H) 2.17 (s, 3H) 2.02 (m, 4H) 1.66 (s, 3H) 1.60 (s, 6H) ppm. 13C-NMR (CDC13) (67.8 MHz) 6 191.0, 163.6, 136.5, 131.3, 127.4, 124.0, 122.4, 40.5, 39.6, 26.6, 25.6, 17.6, 17.5, 15.9 ppm. (2) 4,8,12-Trimethyl-l,3,7,11-trideca- tetraene A suspension of methyltriphenylphosphonium iodide (8.07 g, 0.02 mole) in 61 ml of dry tetrahydrofuran (THF), under argon atmosphere was 20 cooled to 0°C. To this suspension at 0°C was added 9 mL (18 mmol) of phenyllithium (2.0 M in diethyl ether/hexane 30:70) over 10 minutes. After the addition was complete, the reaction mixture containing excess phosphonium salt was warmed to room temperature 25 and stirred for 40 minutes. The reaction mixture was then recooled to 0°C, and a 10 ml THF solution of the Part (1) aldehyde (4.0 g, 0.018 mol) was added over 12 minutes. After stirring for 10 minutes at 0°C, the reaction was warmed to room 30 temperature. The reaction was quenched with CH30H after 2 hours at room temperature. The THF was removed from the reaction mixture to give a slurry ? A h 3 6 u% i I \j ■*>* which was triturated with petroleum ether, and subsequently, filtered through a Celite pad in a sintered glass funnel. The solids were then boiled in petroleum ether and refiltered as above. The 5 resulting yellow oil was passed through 50 g of Florisil (100-200 mesh) eluted with ~400 ml of petroleum ether providing the title tetraene (3.36 g, 86%) as a clear oil after solvent removal.

TLC Silica gel (20% ethyl acetate/hexane) Rf=0.68 10 NMR (CDC13) (270 MHz) 6 6.56 (ddd, 1H, J=17, 12, 6 Hz) .85 (d, 1H, J=12 Hz) .10 (m, 2H) .02 (m, 2H) 2.05 (m, 8H) 1.75 (s, 3H) 1.67 (s, 3H) 1.60 (s, 6H) ppm. 13C-NMR (CDC13) (67.8 MHz) 6 139.3, 135.3, 133.4, 131.2, 125.5, 124.3, 123.9, 114.5, 39.9, 39.7, 26.8, 26.4, 25.6, 17.7, 16.6, 15.9 ppm. (3 ) (E,E)-4,8,12-Trimethy1-3,7,11- tridecatrien-l-ol Neat 2-methyl-2-butene (2.25 g, 0.032 mol) was added to a 1.0 M BH3~THF solution (16.9 ml) at -50°C and under argon. After the addition was complete, the reaction was stirred for 2 hours at 30 0°C. The resulting disiamylborane solution was transferred via cannula over 1 hour to a flask containing a 17 ml THF solution of Part A(2) tetraene (3.36 g, 0.015 mol) under argon atmosphere ^ A /< £ :v -i- o 0 and cooled to 0°C. After the transfer was complete, the reaction was allowed to gradually warm to room temperature, and then it was stirred overnight at room temperature. The reaction mixture was cooled 5 to 0°C, and 5.1 ml of 3N NaOH was added rapidly.

After stirring for 10 minutes, the reaction mixture was cooled in an ice-salt bath and 5.1 ml of 30% H202 was added dropwise. Subsequently, the reaction was warmed to room temperature and stirred for 4 10 hours after which it was diluted with H20, and the resulting aqueous layer was extracted several times with ethyl ether. The combined organic layers were dried over MgSO^. Purification by flash chromatography eluting with 20% ethyl acetate/hexane 15 provided the title alcohol (2.62 g, 74%) as a clear oil.

TLC Silica gel (20% ethyl acetate/hexane) Rf=0.23 IR (Film) 3340 (br), 2965, 2920, 1665, 1440, 1380, 1100, 1050 cm"1.

LH NMR (CDC13) (270 MHz) 65.10 (m, 3H) 3.61 (t, 2H, J=6 Hz) 2.29 (q, 2H, J=6 Hz) 2.03 (m, 8H) 1.67 (s, 3H) 1.65 (s, 3H) 1.60 (s, 6H) ppm. 13C NMR (CDC13) (67.8 MHz) 6 138.8, 135.2, 131.2, 124.3, 123.9, 119.9, 62.4, 30 39.8, 39.7, 31.5, 26.7, 26.5, 25.6, 17.6, 16.1, 15.9 ppm. o !, '■( 7 \ ■>.j R 12- B. (E,E)-[[(4,8,12-Trimethvl-3,7,ll-tri-decatrienyl)oxy]methyljphosphonic acid, diethyl ester To a solution of 1.204 g (5.09 mmol) of 5 Part A homofarnesol in 12 ml of tetrahydrofuran (THF) at -78°C under argon was added over 15 minutes a solution of 3.35 ml (5.34 mmol, 1.05 eq.) of 1.6 M n-butyllithium in hexanes. The resulting solution was stirred at -78°C for 0.5 10 hour, then treated with a solution of 1.68 g (5.60 mmol, 1.1 eq.) of triflate prepared as described in Example 5 Part B, in 8 ml of THF. The reaction mixture was allowed to stir for 0.5 hour at -78°C and 1.5 hours at -20°C (salt-ice bath). The 15 mixture was diluted with ethyl ether, quenched with NHdCl and separated. The organic phase was washed with water and brine, dried over MgSQ^ and evaporated to yield 1.972 g of a clear, colorless oil. Purification by flash chromatography on 200 20 g of silica, eluted with a 3:7 acetone:petroleum ether provided two portions of product, each coeluting with a different impurity. Portion A (556.7 mg) was passed through a second flash chromatographic column of 65 g silica gel eluted 25 with 2:8 acetone:petroleum ether to provide 443.7 mg of impure material. This was further purified by medium pressure chromatography on a Merck Lobar silica gel column (size B), which was eluted with 4:6 ethyl acetate:hexane to obtain 259.8 mg (13%) 30 of desired product. Portion B (864.1 mg) from the first column was rechromatographed on 40 g of silica, eluted with a 4:6 ethyl acetate:petroleum ether to provide 788.1 mg (40%) of desired product Title compound was thus obtained as 1.048 g of a clear oil in 53% yield.

TLC silica gel (1:1 ethyl acetate:hexanes) R^=0.18 IR(CC14) 2980, 2928, 2914, 2868, 2858, 1443, 1391, 1259, 1245, 1119, 1099, 1056, 1032, 968 cm"1. XH NMR (CDC13) (270 MHz) 65.10 (m, 3H) 4.17 (quint, 4H, J=7.1 Hz) 3.78 (d, 2H, J=8.4 Hz) 3.54 (t, 2H, J=7.1 Hz) 2.31 (q, 2H, J=7.1 Hz) 1.9-2.2 (m, 8H) 1.67 (s, 3H) 1.62 (s, 3H) 1.60 (s, 6H) 1.35 (t, 6H, J=7.1 Hz) ppm.

Mass Spec (FAB, + ions) m/e 387 (M+H), 169, 141.

C. (E,E)-[[(4,8,12-Trimethyl-3,7,11-tri-decatrienyl)oxy]methyl]phosphonic acid, monoethvl ester A mixture of 1.001 g (2.59 mmol) of Part B compound, 26 ml of ethanol and 25.9 ml (25.9 mmol, 10 eq.) of 1 M KOH was stirred at 60-70°C under nitrogen for six hours, then cooled and stirred at room temperature for 20 hours. After adjusting to ^pH 6 with 10% HCl, the ethanol was evaporated. The remaining aqueous phase was acidified with 10% HCl and extracted with four 50 ml portions of ethyl acetate. The combined organic layers were washed with 25 ml of 1:1 ^Q: brine and 25 ml of brine, dried over MgS04 and evaporated to yield 9 U ^ 4 "V „ title phosphonic acid 938.3 mg (100%) in the form of a pale yellow oil.

TLC Silica gel (6:3:1 n-C3H7OH:concentrated NH^ri^O) Rf=0.63.

XH NMR(CDC13) (270 MHz) 610.9-11.1 (br, 1H) .10 (m, 3H) 4.18 (quint, 2H, J=7.0 Hz) 3.80 (d, 2H, J=8.4 Hz) 3.54 (t, 2H, J=7.0 Hz) 2.31 (q, 2H, J=7.0 Hz) 1.9-2.1 (m, 8H) 1.68 (s, 3H) 1.61 (s, 3H) 1.60 (s, 6H) 1.35 (t, 3H, J=7.0 Hz) ppm.

D. (E,E)-[[Ethoxy[[4,8,12-trimethyl-3,7,11-tridecatrienyl]oxy]methyl]-20 phosphinyl]methyl]phosphonic acid, dimethyl ester A solution of 936.1 mg (2.59 mmol) of Part C monoethyl, dimethyl triester phosphonic acid in 8 ml of dry CH2C12 at room temperature under argon 25 was treated with 980 p1 (5.18 mmol, 2 eq.) of diethyl(trimethylsilyl)amine. After 1.5 hours, the solvent was evaporated and the residue was twice evaporated from benzene, then dried at high vacuum for 0.5 hour.

The residue was dissolved in 8 ml of CH2Cl2 and two drops of dimethyl formamide (DMF) at 0°C under nitrogen and treated with 410 pi (4.66 mmol, 1.8 eq) of oxalyl chloride. The mixture was allowed to warm to room temperature and stirred for two hours. The solvent was evaporated and the residue was twice evaporated from benzene, then dried at high vacuum for 0.5 hour.

The anion solution was prepared by adding over five minutes a solution of 3.50 ml (5.57 mmole, 2.15 eq.) of 1.6 M n-butyllithium in hexanes to a solution of 620 pi (5.70 mmole, 2.2 eq.) of dimethyl methylphosphonate in 15 ml of tetrahydrofuran (THF) at -78°C under argon, followed by stirring for 0.5 hour.

A solution of the phosphonic acid chloride prepared above in 6 ml of THF at -78°C under argon was added quickly via cannula to the anion solution. The resulting mixture was stirred at -78°C for 1.5 hours, then quenched with 300 pi (5.2 mmol, 2 eq.) of glacial acetic acid and allowed to stir for ten minutes. Approximately 3 ml of saturated NH4C1 was added and the cold bath was removed. Once at room temperature, 10 ml of H20 was added and the mixture was extracted with five 40 ml portions of CH2C12- The combined organic phases were washed with 30 ml of 1:1 H20:brine and 30 ml of brine, dried over MgSO^ and evaporated to obtain 1.241 g of crude coupling product. Purifica tion by flash chromatography on 120 g of silica gel eluted with 2:98 CH3OH:CH2Cl2 provided 501.7 mg (42%) of title product.

TLC Silica gel (5:95 CH30H:CH2Cl2) Rf=0.17 IRfCCl..) 2956, 2917, 2856, 1447, 1257, 1231, 1166, -1 1111, 1063, 1037, 960, 842 cm 1. <\ /. 1.

H NMR(CDC13) (270 MHz) 65.10 (m, 3H) 4.19 (d quint, 2H, J=2.6, 7.4 Hz) 3.82 (d, 3H, J=10.6 Hz) 3.80 (d, 3H, J=ll.6 Hz) 3.7-3.9 (m, 2H) 3.55 (dt, 2H, J=1.3, 6.9 Hz) 2.4-2.7 (m, 2H) 2.30 (quint, 2H, J=6.9 Hz) 1,9-2.1 (m, 8H) 1.68 (s, 3H) 1.62 (s, 3H) 1.60 (s, 6H) 1.36 (t, 3H, J=7.4 Hz) ppm.

Mass Spec (CI-C^/^O, + ions) m/e 505 (M+C^H^), 493 (M+C2H5), 465 (M+H).

Example 8 (E,E)-[[Hydroxy[[4,8,12-trimethyl-3,7,11-trideca-20 trienyl]oxy]methyl]phosphinyl]methyl]phosphonic acid, trisodium salt To a solution of 485.9 mg (1.05 mmol) of Example 7 triester and 310 pi (2.35 mmol, 2.25 eq.) of 2,4,6-collidine in 5 ml of dry CH2Cl2 at 25 room temperature under nitrogen in the dark was added dropwise 620 jj 1 (4.71 mmol, 4.5 eq. ) of bromotrimethylsilane. This solution was allowed to stir for seven hours, then evaporated twice with benzene and dried at high vacuum for 0.5 30 hour. The residue was treated with 8.40 mL (8.40 mmol, 8 eq.) of 1 M NaOH and the aqueous solution was lyophilized overnight. Purification was by chromatography on a 12 cm height x 2.5 cm diameter 2 4 4 3 6 column of CHP20P packed with water and eluted with 100 ml of H20 followed by a gradient created by the gradual addition of 400 ml of CH^CN into 400 ml of H20. Approximately 8-10 ml fractions were 5 collected every 1.2 minutes. Fractions 52-55 and 58-59 were combined, filtered, evaporated, lyophilized overnight and pump-dried for eight hours to provide 294.0 mg (59%) of title salt as a fluffy white lyophilate. (In addition, fractions 10 56 and 57 were combined, filtered and lyophilized to obtain 159.8 mg (32%) of title salt containing a trace impurity by TLC).

TLC Silica gel (5:4:1 n-C^OH:con. NH^HjO) Rf=0.28 IR(KBr) 3000-3500(br), 2966, 2916, 2856, 1442, 15 1381, 1178, 1148, 1093, 976, 873, 812, 798, 783, 761, 741, 490, 478 cm'1. 1H NMR(D20) (400 MHz) 55.13 (m, 3H) 3.63 (d, 2H, J=6.5 Hz) 3.54 (t, 2H, J=7.3 Hz) 2.32 (q, 2H, J=6.5 Hz) 1.9-2.1 (m, 10H) 1.65 (s, 3H) 1.63 (s, 3H) 1.58 (s, 3H) ppm. 31P-NMR (D20) 534.7 (d, J=10.3 Hz) .7 (d, J=10.3 Hz) ppm.

Mass Spec (FAB, + ions) m/e 497 (M+Na), 475 (M+H), 453 (M+2H-Na).

Anal Calcd for c18H3106P2Na3•0.86 H20 (MW 489.76): C, 44.14; H, 6.73; P, 12.65 Found: C, 44.04, H, 6.85; P, 12.90. cTj / /. T z ^ £t j Example 9 (E,E)-[[Hydroxy[[(3,7,ll-trimethyl-2,6,10-dodec-atrienyl)oxy]methyl]phosphinyljmethyl]phosphonic acid, monomethyl ester, dipotassium salt The Example 3 triester (0.321 mmol, 0.140 g) was stirred under argon in 1.6 mL of 1 N KOH. The reaction mixture was heated in a 55°C oil bath for 24 hours. The reaction mixture was cooled to room temperature and applied directly to a 10 chromatography column containing CHP20P resin (12 cm x 2.5 cm/equilibrated with 1000 ml distilled H20). The column was eluted with 100 ml of H^O, followed by a gradient created by gradual addition of 400 ml of CH^CN to 400 ml of H20. Fractions 15 were collected every 1.7 minutes (^10 ml).

Fractions 54-63 were combined, evaporated in vacuo, lyophilized, and pumped under high vacuum over P2°5 ^or ^ hours to afford 0.115 g (0.237 mmol, 74% yield) of the title dipotassium 20 salt as a white lyophilate.

TLC:Silica gel, (7:2:1 n-C^OH/con. NH3/H20) Rf=0.45 1H NMR (400 MHz, D20) 65.36 (t, 1H, J=6.96 Hz) .14 (m, 2H) 4.08 (d, 2H, J=6.96 Hz) 3.57 (d, 2H, J=6.96 Hz) 3.51 (d, 3H, J=ll Hz) 2.12-1.95 (m, 10H) 1.66 (s, 3H) 1.63 (s, 3H) 1.57 (s, 6H) ppm. 9 A A 3 6 13C NMR (67.8 MHz, D20) 5144.16, 137.60, 134.45, 125.46, 125.30, 120.61, 70.14 (d, J=11.35 Hz), 69.39 (d, J=113.54 Hz), 52.35 (d, J=5.6 Hz), 39.9, 39.8, 28.34 (dd, J=79.48 Hz, 124.9 Hz), 26.81, 5 26.67, 25.89, 18.02, 16.74, 16.32 ppm.

Mass Spec (FAB, + ions) m/e 485 (M+H) IR(KBr) 2966, 2924, 2955, 1446, 1222, 1191, 1151, 1107, 1065, 1053, 787, 741 cm"1.

Anal Calcd for cigH32°6P2K2•0.14 moles H20: 10 C, 44.39; H, 6.68; P, 12.72 Found: C, 44.38; H, 6.74; P, 12.74 Example 10 (E,E)-[[Hydroxy[[(3,7,ll-trimethyl-2,6,10-dodec-15 atrieny1)oxy]methyl]phosphinyl]methyl]phosphonic acid, trisodium salt A sample of 660 mg (1.42 mmol) of Example 1 triester was treated with 0.47 ml (3.54 mmol) of 2,4,6-collidine and 0.94 ml (7.09 mmol) of 20 bromotrimethylsilane in precisely the same manner on identical scale as in the preparation of the Example 2 tripotassium salt. For the salt formation, 8 ml of 1M NaOH was substituted for KOH, and similarly, CHP20P chromatography gave 630 25 mg (94%) of title salt as a fluffy white lyophilate. TLC, 1H NMR, 31P NMR, 13C NMR, IR and Mass Spec is essentially identical to the Example 2 tripotassium salt.

Anal Calcd for C^H^Na^P^g + 0.46 mol H20: 30 C, 43.57; H, 6.44; P, 13.22 Found: C, 43.71; H, 6.46; P, 13.40 24 4 3 Example 11 (E,E) - [[Ethoxyf[(5,9,13-trimethyl-4,8,12-tetra-decatrienyl)oxy]methyl]phosphinyl]methyl]phosphonic acid, dimethyl ester A. Bishomofarnesol (1) (E,E)-3,7,ll-Trimethyl-2,6,10-dodecatrienyl bromide A solution of 1.00 g (4.5 mmol) of (E,E)-farnesol (Aldrich, further purified by flash 10 chromatography) in 10 ml of distilled ether at 0°C under argon in the dark was treated dropwise with a solution of 195 pL (2.05 mmol, 0.45 eq.) of PBr^ in 2 ml of ether. The resultant mixture was stirred at 0°C for one hour, then quenched with 15 water and separated. The organic phase was washed with 5 ml of HjO, 5 ml of saturated NaHCO^, and 5 ml of brine, dried over Na2S04 and evaporated to give 1.26 g (98%) of crude bromide as a clear oil. TLC Silica (2:8 ethyl acetate:Hexane) Rf=0.69. 20 XH NMR (CDC13, 270 MHz) 65.52 (t, 1H, J = 8.5 Hz) .08 (m, 2H) 4.01 (d, 2H, J=8.5 Hz) 1.9-2.2 (m, 8H) 1.73 (s, 3H) 1.68 (s, 3H) 1.60 (s, 6H) ppm. (2) (E,E)-5,9,13-Trimethyl-4,8,12-tetradec-30 atrienoic acid, 1,1-dimethylethyl ester To a solution of 9.60 ml (68.5 mmol, 1.5 eq.) of diisopropylamine in 100 ml of THF at -78°C under argon was added 28.2 ml (45.0 mmol, 1.0 eq. ) 9 A /> 7s & L 4 i J ij of 1.6 M n-butyllithium in hexanes over 20 minutes. After warming to 0°C for 15 minutes, the solution was recooled to -78°C and 6.05 ml (45 mmol, 1.0 eq) of t-butyl acetate was added over 20 5 minutes. After an additional 15 minutes, 16.0 ml (92 mmol, 2.05 eq.) of hexamethylphosphoramide (HMPA) was added, followed by a solution of 12.53 g (45.0 mmol) of Part A(l) farnesyl bromide in 100 ml of THF over 20 minutes. The reaction was stirred at 10 -78°C for 2.5 hours, cruenched with saturated NH.Cl and allowed to warm to room temperature. After diluting with 400 ml of ethyl acetate, the mixture was washed with four 100 ml portions of water, and 200 ml of brine, dried over MgSO^ and evaporated 15 to provide 12.96 g of crude product as a yellow oil. Purification by flash chromatography on 1 kg of silica gel, eluted with 1:9 ethyl acetate:petroleum ether afforded 9.39 g (65%) of title compound as a pale yellow oil.

TLC Silica gel (2:98 ethyl acetate:hexane) R^=0.16. IR(neat) 2977, 2925, 2857, 1733, 1452, 1368, 1258, 1149 cm"1.

XH NMR(CDC13) (270 MHz) 55.10 (m, 3H) 2.25 (m, 4H) 1.9-2.1 (m, 8H) 1.68 (s, 3H) 1.62 (s, 3H) 1.59 (s, 6H) 1.44 (s, 9H) ppm.

Mass Spec (CI-CH4/N20) (+ ions) m/e 165 (M+H-C4H8), 247, 183, 137, 68, 57. (- ions) m/e 319 (M-H), 279, 251, 100. ? 4 4 3 SUM X * I*.' (3) Bishomofarnesol To a stirred solution of 5.00 g (15.6 mmol) of Part (2) compound in 45 ml of dry diethyl ether at 0°C under argon was added 592 mg (15.6 mmol, 1 5 mol - eq.) of lithium aluminum hydride, and the resulting suspension was stirred at room temperature for 20 hours. After cooling to 0°C, the reaction was quenched by treating with 5 ml of H20, 5 ml of 15% NaOH, and 15 ml of 1^0 and 10 stirring the suspension for \ hour. After adding Na2S04, the slurry was filtered through Celite, washing well with diethyl ether and evaporated to obtain 3.62 g of crude product. Purification by flash chromatography on 300 g of silica gel, eluted 15 with 1:9 ethyl acetaterpetroleum ether provided 3.516 g (90%) of bishomofarnesol as a colorless liquid.

TLC Silica gel (2:8 ethyl acetate:hexane) R^=0.19 IR(neat) 3330, 2964, 2926, 2873, 2958, 1448, 1384, 20 1107, 1059, 401 cm"1. 1H NMR(CDC13) (270 MHz) 65.10 (m, 3H) 3.63 (t, 2H, J=6.5 Hz) 1.9-2.2 (m, 10H) 1.68 (s, 3H) 1.62 (2, 3K) 1.60 (s, 7H) ppm.

Mass Spec (CI-CH4/N20, + ions) m/e 251 (M+H), 249 (M+H-H2), 137, 123, 109, 69. f\ ; / "7 Q X / k h. A t 1 ^ k v- v w B. (E,E)-[ [(5,9,13-Trimethyl-4,8,12-tetradecatrien-l-yl)oxy]methyl]phosphonic acid, diethyl ester To a solution of 1.198 g (4.80 mmol) of 5 Part A bishomofarnesol in 25 ml of tetrahydrofuran at -78°C under argon was dropwise added 3.00 ml (4.80 mmol) of 1.6 M n-butyllithium in hexanes over ten minutes. After 0.5 hours at -78°C, a solution of 1.299 g (4.32 mmol, 0.9 equiv.) of 10 Example 5 Part B triflate in 20 ml of tetrahydrofuran was added. The reaction mixture was warmed to 0°C and stirred for two hours.

After diluting with 40 ml of diethyl ether and quenching with 25 ml of saturated NH4C1, the 15 layers were separated and the aqueous phase extracted with 180 ml of diethyl ether. The organic layers were combined, washed with 20 ml of brine, dried over MgSO^, and evaporated to yield 1.814 g of crude product. Purification by flash 20 chromatography on 180 g of silica gel, eluted with 2:8 acetone:hexane provided 721.2 mg (42%) of pure title diester as a colorless oil. TLC silica gel (1:1 ethyl acetate:hexane) R^=0.27. IR(CC1a) 2980, 2929, 2914, 2866, 2859, 1443, 1392, 25 1384, 1378, 1260, 1243, 1165, 1120, 1099, 1057, 1031, 968, 812, 798, 795, 773 cm"1. 1H NMR (CDC13) (270 MHz) 65.11 (br s, 3H) 4.17 (quint, 4H, J=7.1 Hz) 30 3.76 (d, 2H, J=8.8 Hz, Hia) 3.55 (t, 2H, J=6.3 Hz) 1.9-2.1 (m, 10H) 1.68 (s, 3H) 2 4 4 3 1.60 (m, 11H) 1.35 (t, 6H, J=7.1 Hz) ppm.

Mass Spec (CI-O^/^O, + ions) m/e 401 (M+H), 281, 253 .

C. (E,E)-[[(5,9,13-Trimethyl-4,8,12-tetradecatrien-l-yl)oxy]raethyl]phosphonic acid, monoethyl ester A solution of 711.1 mg (1.75 mmol) of Part 10 B diester in a mixture of 17.5 ml (17.5 mmol, 10 eq) of 1 M NaOH and 17.5 ml of ethanol was heated at 65-70°C for 2.5 hours, then cooled to room temperature. The mixture was adjusted to ^pH 7 with 1 M HCl and the ethanol was evaporated. The 15 aqueous solution was further acidified and extracted with four 30 ml portions of ethyl acetate. The combined organic phases were washed with 20 ml of 50% brine and 20 ml of brine, dried over MgSO^ and evaporated to obtain 652.4 mg (100%) of crude 20 title phosphonic acid mono ester.

TLC: Silica gel (8:1:1 n-C-^OH: con.NH3 :H20) Rf=0.43 1H-NMR(CDC13) (270 MHz) 55.10 (br s, 3H) 4.17 (t, 2H, J=7.1 Hz) 3.76 (d, 2H, J=8.8 Hz) 3.55 (t, 2H, J=6.6 Hz) 1.9-2.1 (m, 10H) 1.68 (s, 3H) 1.60 (m, 11H) 1.34 (t, 3H, J=7.1 Hz) ppm. 24 4 3 D. (E,E)-[[Ethoxy[[(5,9,13-trimethyl-4,8,12-tetradecatrienyl)oxy]methyl]-phosphinyl]methyl]phosphonic acid, dimethyl ester A solution of 652 mg (1.75 mmol) of Part C phosphonic monoester in 5 ml of Crl^Cl^ at room temperature under argon was treated with 665 pi (3.50 mmol, 2 equiv.) of N,N-diethyl(trimethylsilyl)-amine and stirred for two hours. The solvent was 10 evaporated, and the residue was twice evaporated from benzene, then dried at high vacuum for 0.5 hours.

The residue was dissolved in 5 ml of CI^C^ and one drop of dimethylformamide at 0°C under 15 nitrogen and was treated dropwise with 275 pi (3.15 mmol, 1.8 eq) of oxalyl chloride over five minutes. The solution was allowed to warm to room temperature and stirred for two hours. The solvent was evaporated, and the residue was twice 20 evaporated from benzene, then dried at high vacuum for 0.5 hours.

The anion solution was prepared by treating a solution of 420 pi (3.85 mmol, 2.2 equiv.) of dimethyl methylphosphonate in 5 ml of 25 tetrahydrofuran at -78°C under argon with a solution of 2.35 ml (3.76 mmol, 2.15 equiv.) of 1.6 M n-butyllithium in hexanes over ten minutes. The solution was allowed to stir for 0.5 hours, then a solution of the acid chloride prepared 30 above in 3 ml of tetrahydrofuran was rapidly added. The resulting orange solution was stirred at -78°C for 1.5 hours, then quenched with a solution of 200 pi (3.50 mmol, 2 equiv.) of acetic 2 4 4 3 acid in 1 ml of tetrahydrofuran. After stirring for ten minutes, 1 ml of saturated NH^Cl was added and the cold batch was removed. Water was added (■^5 ml) and the mixture was extracted with five 20 5 ml portions of CH2C12. The combined organic phases were washed with 20 ml of 50% brine and 20 ml of brine, dried over HgSO^ and evaporated to give 781.1 mg of a yellow oil. Purification required two chromatographies, the first on 75 g 10 of silica, eluted with 3:97 CH3OH:CH2Cl2; the second on 50 g of silica, eluted with 1.5:98.5 CH30H:CH2C12, affording 335.4 mg (40%) of title compound as a clear, colorless oil.

TLC:Silica gel (5:95 CH3OH:CH2Cl2) Rf=0.14 15 IR(CC14) 2954, 2927, 2917, 2855, 1447, 1391, 1383, 1377, 1365, 1258, 1231, 1184, 1165, 1113, 1063, 1036, 960, 842, 801, 790, 770, 759, 752, 737 cm"1. XH NMR(CDC13) (270 MHz) 65.10 (br s, 3H) 4.18 (m, 2H) 3.82 (d, 3H, J=11.0 Hz) 3.80 (d, 3H, J=11.0 Hz) 3.7-3.95 (m, 2H) 3.56 (t, 2H, J=6.3 Hz) 2.51 (m, 2H) 1.9-2.1 (m, 10H) 1.68 (s, 3H) 1.60 (s, 11H) 1.36 (t, 3H, J=7.1 Hz) ppm.

Mass Spec (CI-CK^/H^O, + ions) m/e 479 (M+H). 2.4 4 3 Example 12 (E,E)-[[Hydroxy[[(5,9,13-trimethyl-4,8,12-tetra-decatrienyl )oxy] methyl ] phosphinyl ] methyl ] phosphonic acid, trisodium salt A solution of 318.6 mg (0.67 mmol) of Example 11 triester in 3.5 ml of CH2Cl2 at room temperature under argon was treated with 220 pi (1.68 mmol, 2.5 equiv.) of 2,4,6-collidine and 445 pi (3.35 mmol, 5 equiv.) of bromotrimethvlsilane 10 and stirred for six hours. The solvent was evaporated, and the residue was treated with 6.7 ml (6.70 mmol, 10 equiv.) of 1 M NaOH and lyophilized. The crude product was purified by chromatography on a 12 cm height x 2.5 cm 15 diameter column of CHP20P resin loaded in water and eluted with 100 ml of water, followed by a gradient created by the gradual addition of 400 ml of 7:3 CH^CN:water into 400 ml of water, collection of approximately 10 ml fractions every 20 1.5 minutes. Fractions 43-54 were combined, evaporated, lyophilized and pump-dried for six hours to afford 240.1 mg (73%) of title product as a white lyophilate.

TLC silica gel (4:4:1 n-C^OH: con. NH3:H20) Rf=0.33 25 IR(KBr) 3000-3700 (br), 2964, 2925, 2859, 1661, 1638, 1177, 1148, 1096, 975, 793 cm"1. 1H-NMR (D20) (400 MHz) 55.17 (t, 1H, J=6.4 Hz) .10 (m, 2H) 3.57 (m, 4H) 1.9-2.1 (m, 12H) 1.64 (s, 3H) 1.59 (s, 3H) 244363 1.57 (s, 6H) 1.5-1.7 (m, 2H) ppm. 31P-NMR (D20) (36.2 MHz) 632.1 (d, J=ll.5 Hz) 13.1 (d, J=ll.5 Hz) ppm.

Mass Spec (FAB, + ions) m/e 489 (M+H), 467 (M+2H-Na), 445 (M+3H-2Na) Anal calcd for C^H^OgP^ 0.73 H20: C, 45.51; H, 6.92; P, 12.35 10 Found: C, 45.29; H, 7.06; P, 12.59 Example 13 (E,E)-[[Hydroxy[[(3,7,ll-trimethyl-2,6,10-dodec-atrieny1)amino]methyl]phosphinyl]methyl]phosphonic acid, disodium salt A. (E,E)-3,7,ll-Trimethyl-2,6,10-dodec-atrien-l-amine, monohydrochloride (1) 2 — (3,7,ll-Trimethyl-2,6,10-dodeca-trienyl)-lH-isoinaole-l,3(2H)-dione 20 A solution of 2.00 g (9.0 mmol) of (E,E)- farnesol in 20 ml of dry diethyl ether at 0°C under argon in the dark was treated dropwise with a solution of 735 pi (4.0 mmol, 0.45 eq.) of PBr^ in 4 ml of dry diethyl ether. The resultant mixture 25 was stirred at 0°C for one hour, then quenched with H20 and separated. The organic phase was washed with 15 ml of NaHC03, 15 ml of H20, and 15 ml of brine, dried over MgSO^ and evaporated to provide 2.47 g of crude farnesyl bromide as a clear oil. 30 A solution of 2.47 g of the above farnesyl bromide in 20 ml of dry dime thy formamide (DMF) at room temperature under argon was treated with 1.83 g 2 4 4 3 6 3 (9.9 mmol, 1.1 eg.) of potassium phthalimide and stirred for 3 hours at room temperature. The solvent was removed under reduced pressure, the residue was triturated with 150 ml of ethyl ether, 5 and the precipitate was filtered off. The ethereal solution was washed with 50 ml of i^O and 50 ml of brine, dried over MgSO^ and evaporated to yield 2.96 g of crude title compound as a milky oil. Purification by flash chromatography on 300 g of 10 Merck 9385 silica, eluted with 7:93 ethyl acetate:petroleum ether afforded 2.56 g (81%) of the desired product as a colorless oil.

TLC Silica gel (2:8 ethyl acetate:hexane) R^=0.37 IR(neat) 2967, 2920, 2856, 1772, 1716, 1468, 1432, 15 1394, 13G6, 1325, 1112, 1088, 1073, 947, 721, 531 cm 1H NMR (CDC13) (270 MHz) 67.82 (da, 2H, J=3.0, 5.5 Hz) 7.68 (dd, 2H, J=3.0, 5.5 Hz) 20 5.27 (t, 1H, J=7.0 Hz) .05 (d, 2H, J=7.0 HZ) 4.27 (d, 2H, J=7.0 Hz) 1.9-2.1 (m, 8H) 1.83 (s, 3H) 1.66 (s, 3H) 1.56 (s, 6H) ppm.

Mass Spec (CI-CH4/N20, + ions) m/e 392 (M+C3H5), 380 (M+C2H5), 352 (M+H), 296, 284, 270, 228, 216. (2) Farnesyl amine A solution 2.50 g (7.1 mmol) of Part (1) compound in 15 ml of absolute ethanol at room temperature under argon was treated with 1.9 ml 244363 (35.57 mmol, 5.0 eq.) of methyl hydrazine and stirred for 2 hours at room temperature and 4 hours at reflux. After cooling and the addition of 7.1 ml (7.1 mmol, 1.0 eq.) of 1M NaOH, the 5 ethanol was removed under reduced pressure. The residue was extracted with 350 ml of ethyl ether and the ether layer washed with 100 ml of 1M NaOH, 50 ml of H20 and 50 ml of brine, dried (MgSO^) and evaporated to obtain 1.45 g of crude product. 10 Purification by bulb-to-bulb distillation at 120°C/0.005 mm provided 1.405 g (89%) of title compound as a colorless oil.

TLC:Silica gel (8:1:1 n-propanol:con.NH3:H20) Rf=0.64 IR(neat) 3291, 2967, 2923, 2856, 1574, 1483, 1453, 15 1383, 1378, 1347, 1288, 819, 777 cm"1.

XH NMR (CDC13) (270 MHz) 55.26 (t, 1H, J=7.0 Hz) .10 (br, 2H) 3.27 (d, 2H, J=7.0 Hz) 1.9-2.1 (m, 8H) 1.68 (s, 3H) 1.63 (s, 3H) 1.60 (s, 6H) 1.20 (br s, 2H) ppm.

Mass Spec (CI-CH4/N20, + ions) m/e 443 (2M+H), 222 (M+H), -205, 137. (3) (E,E)-3,7,ll-Trimethyl-2, 6,10-dodec-atrien-l-amine, monohydrochloride 30 A solution of 254.5 mg (1.15 mmol) of Part (2) farnesyl amine in diethyl ether was treated with HCl-saturated diethyl ether and the resulting precipitate was filtered off and dried for 20 hours under vacuum to obtain 197.3 mg (67%) of a white solid, m.p. 121-122°C.

TLC Silica gel (8:1:1 n-c3h?oh:con.nh3 :H20) Rf=0.56 IR(KBr) 2983, 2929, 1600, 1464, 1384, 1106, 890 5 cm 1. 1H NMR (CD3OD) (400 MHz) 65.31 (t, 1H, J=7.5 Hz) .13 (t, 1H, J=7.5 Hz) .09 (t, 1H, J=7.5 Hz) 3.56 (d, 2H, 3-1.5 Hz) 2.0-2.2 (m, 6H) 1.98 (t, 2H, J=7.5 Hz) 1.76 (s, 3H) 1.66 (s, 3H) 1.61 (s, 3H) 1.60 (s, 3H) ppm. 13C-NMR (CD3OD) (67.8 MHz) 5145.8, 136.7, 132.1, 125.3, 124.7, 116.6, 40.8, 40.6, 38.3, 27.8, 27.2, 25.9, 17.8, 16.6, 20 16.1 ppm.

Mass Spec (CI-CH4/N20, + ions) m/e 222 (M+H-HCl), 205, 137, 81.

Anal Calcd for C15H27N-HC1 (MW 257.849): C, 69.87; H, 10.95; N, 5.43; Cl, 13.75 25 Found: C, 69.96; H, 10.87; N, 5.39; Cl, 13.78 B. (E,E)-[[(3,7,ll-Trimethyl-2,6,10-dodec-atrienyl)amino]methyl]phosphonic acid, diethyl ester To a stirred solution of 1.88 g (7.29 mmol) of Part A hydrochloride salt and 2.9 ml (16.6 mmol) of diisopropylethylamine in 30 ml of CH2C12 under argon at 0°C was added 2.46 g (6.63 mmol) of 0 /« R / S- H o 9 2- Example 5 Part B trifluororaethylsulfonate in 5 ml of CH2C12 over 45 minutes. After 1.5 hours at 0°C, the reaction was diluted with 100 ml of CH2C12, washed with 20 ml of 2 M Na2C03, and the 5 aqueous layer was back-extracted with 20 ml of CH2C12. The combined organic layers were dried (MgSO^) and evaporated to provide 2.87 g of a yellow oil. The crude product was flash chromatographed on 200 g of silica gel packed in 10 12.5:87.5 and eluted with 25:75 acetone:hexane to provide 2.18 g (89%) of pure title amine as a pale yellow liquid.

TLC silica gel (50:50 acetone:hexane) R^=0.33 IR (CC14) 2980, 2928, 2913, 2856, 1444, 1241, 15 1098, 1058, 1031, 964 cm"1.

XH NMR (CDC13) 55.22 (t, 1H, J=7 Hz) .08 (m, 2H) 4.15 (quint, 4H, J=7 Hz) 20 3.29 (d, 2H, J=6.8 Hz) 2.96 (d, 2H, J=12.6 Hz) 2.05 (m, 8H) 1.68 (s, 3H) 1.64 (s, 3H) 1.60 (s, 6H) 1.43 (t, 6H, J=7 Hz) ppm.

Mass Spec (CI-CH4/N20, + ions) m/e 372 (M+H), 168.

C. (E,E)-[[[(1,1-Dimethvlethoxy)carbonyl]-30 (3,7,ll-trimethyl-2,6,10-dodecatrienyl)- amino]methyl]phosphonic acid, diethyl ester To a solution of 1.045 g (2.81 mmol) of Part B amine in 9 ml of CE^C^ under argon was added 860 mg (3.94 mmol) of di-tert-butyl dicarbonate. The solution was allowed to stir for 5 3 hours at room temperature and evaporated, and the residue was flash chromatographed on 70 g of silica gel eluted with 50:50 ethyl acetate:hexane to provide 1.28 g (96%) of title compound as a thick, colorless oil.

TLC Silica gel (50:50 ethyl acetate:hexane) R^=0.24. IR(CC14) 2979, 2929, 2915, 2856, 1698, 1477, 1452, 1421, 1367, 1240, 1161, 1055, 1030, 968 cm"1. le NMR (CDC13) 65.09 (m, 3H) 4.13 (quint, 4H, J=7 Hz) 4.02 (br d, 2H, J=5.8 Hz) 3.63 (br d, 2H, J=8.4 Hz) 2.10 (m, 8H) 1.69, 1.68 (two s, 3H each) 20 1.60 (s, 6H) 1.47 (s, 9H) 1.33 (t, 6H, J=7 Hz) ppm.

Mass Spec (CI - CH^/N^O, + ions) m/e 512 (M+C^H^), 500 (M+C2H5), 472 (M+H), 372, 212, 168.

D. (E,E)-[[[(1,1-Dimethylethoxy)carbonyl]-(3,7,ll-trimethyl-2,6,10-dodecatrienyl)-amino]methyl]phosphonic acid, monoethyl ester A solution of 595 mg (1.26 mmol) of Part C compound in 7 ml of ethanol was treated with 7 ml of 1 M KOH and heated to reflux for 6 hours. The ethanol was evaporated; the residue was stirred 0 /. A ~ R with CH2C12, and was acidified with 10% HCl. The organic layer was washed with brine, dried (MgSO^) and evaporated to provide 564.9 mg (100%) of title acid as a colorless oil.

TLC Silica gel (7:2:1 n-C,H_OH:con NH~:H,0) Rf=0.62. 1H NMR (CDC13) 65.10 (m, 3K) 4.13 (quint, 2H, J=7 Hz) 4.02 (br, 2H) 3.60 (br, 2H) 2.08 (m, 8H) 1.68 (s, 6H) 1.60 (s, 6H) 1.46 (s, 9H) 1.33 (t, 3H, J=7 Hz) ppm.

E. (E,E)-[[[(1,1-Dimethylethoxy)carbonyl]-(3,7,ll-trimethyl-2,6,10-dodecatrienyl)-amino]methyl]phosphonic acid, ethyl 4-nitrophenyl ester To a solution of 327.4 mg (0.75 mmol) of Part D acid in 4 ml of dry pyridine at room temperature under argon was added 106 mg (0.76 mmol) of p-nitrophenol and 9 mg (0.074 mmol) of 25 4-dimethylaminopyridine followed by 198 mg (0.959 mmol) of dicyclohexyl carbodiimide (DCC) in 3 ml of pyridine. The reaction was allowed to stir for 24 hours at room temperature, when another 91 mg (0.44 mmol) of DCC was added, and the reaction was 30 heated at 45-50°C for 5 hours. The reaction was allowed to cool to room temperature, filtered to remove the precipitate and the pyridine was evaporated. The residue was taken up in ethyl acetate, stirred with 10% HCl, and the two-phase mixture was filtered to remove additional precipitate. The organic layer was separated, washed with 10% HCl, water and brine, dried 5 (MgSO^), and evaporated to provide an oil containing some suspended solid. The mixture was triturated with diethyl ether, filtered once more, and evaporated to provide 409 mg of a colorless oil. Rapid flash chromatography on 40 g of pH 4 buffered 10 silica gel packed in 10:90 and eluted with 20:80 ethyl acetate:hexane gave 357.1 mg of a colorless oil. ^H NMR indicated that this was a 5:1 mixture of desired title p-nitrophenyl ester and p-nitrophenol, for a corrected yield of 80%. 15 TLC Silica gel (40:60 ethyl acetate:hexane) R^=0.38 for title p-nitrophenyl ester; 0.28 for p-nitrophenol.

IR (CC14) 2979, 2928, 1699, 1593, 1527, 1492, 1417, 1391, 1368, 1345, 1286, 1245, 1223, 1161, 20 1037, 921 cm"1.

XH NMR (CDC13) 68.22 (d, 2H, J=10 Hz) 7.40 (d, 2H, J=10 Hz) .09 (m, 3H) 4.27 (m, 2H) 4.06 (br, 2H) 3.86 (br d, 2H, J=8.4 Hz) 2.07 (m, 8H) 1.69, 1.68 (two s, 3H each) 1.60 (s, 6H) 1.44 (s, 9H) 1.35 (t, 6H, J=7 Hz) ppm. y *11 *7 Q .1 n Mass Spec (Cl - CH4, + ions) m/e 565 (M+H), 375, 261, 205.

F. (E,E)-[[[[[(1,1-Dimethvlethoxy)-5 carbonyl](3,7,ll-trimethyl-2,6,10- dodecatrienyl)amino]methyl]ethoxyphos-phinyl]methyl]phosphonic acid, dimethyl ester To a stirred solution of 51 pi (0.468 mmol) 10 of dimethyl methylphosphonate m 2 ml of tetrahydrofuran (THF) at -78°C ur.cer argon was added 0.28 ml (0.449 mmol) of 1.6 M n-butyllithium in hexane over 5 minutes. After 20 minutes, the 105 mg (0.185 mmol) of Part E p-nitrophenyl ester 15 in 2 ml of THF was added over 5 minutes to give a yellow solution. The reaction was allowed to stir for 30 minutes at -78°C followed by warming to 0°C for 30 minutes. The reaction was quenched with 50 pi (0.874 mmol) of acetic acid in 1 ml of diethyl 20 ether. The mixture was partitioned between ethyl acetate and saturated NH^Cl, the organic layer was washed with saturated NaHC03 and brine, dried (MgSO^) and evaporated to provide 125.3 mg of a pale yellow oil. The crude product was 25 chromatographed on 10 g of silica gel, eluted with 3:97 CH3OH:CH2Cl2, and the impure fractions were repurified, eluting 2:98 CH30H:CH2Cl2. The pure fractions were combined to provide 85.5 mg (84%) of a pure title compound as a colorless oil. 30 TLC Silica gel (5:95 CH3OH:CH2Cl2) Rf=0.26 IR (CCl^) 2978, 2928, 1695, 1453, 1420, 1391, 1367, 1256, 1230, 1160, 1063, 1036 cm"1. n t> uv tw C J £ 1H NMR (CDC13) 65.10 (m, 3H) 4.24 (quint, 2H, J=7 Hz) 4.03 (br d, 2H, J=5.5 Hz) 5 3.82, 3.80 (two d, 3H each, J=ll Hz) 3.72 (br m, 2H) 2.53 (br t, 2H, J=17.5 Hz) 2.06 (m, 8H) 1.70, 1.68 (two s, 3H each) 10 1.60 (s, 6H) 1.47 (s, 9H) 1.35 (t, 3H, J=7 Hz) ppm. 31P NMR (CDC13) 640.6 (br) 22.9 (d, J=11.7 Hz) ppm.

Mass Spec (CI-CH4/N20, + ions) 590 (M+C3H5), 578 (M+C2H5), 550 (M+H).

G. (E,E)-[[Hydroxy[[(3,7,11-trimethyl-20 2,6,10-dcdecatrienyl)amino]methyl]- phosphinyl]methyl]phosphonic acid, disodiuro salt To a stirred solution of 581 mg (1.06 mmol) of Part F compound in 5 ml of dry CE^Cl^ at 0°C 25 under argon was added 0.42 ml (3.18 rnmol) of 2,4,6-collidine followed by 0.90 ml (6.34 mmol) of iodotrimethylsilane (TMSI). After 2.5 hours at 0°C, the reaction was allowed to warm to room temperature and stirred for 4.5 hours. Additional 30 portions of 2,4,6-collidine (0.14 ml, 1.06 mmol) and TMSI (0.30 ml, 2.12 mmol) were added at this point, and again after another 16 hours at room temperature. Seven hours after the final addition ij i- < & u 3 of reagents, 5 ml of dry CH^C^ was added, and the reaction was allowed to stir for 18 hours. The solvent was evaporated, the dark residue was dissolved in 10 ml of CHgOH, and after 15 minutes, 5 was treated with 10 ml of 1 M NaOH and 20 ml of water. A precipitate formed, and the reaction was stirred for four hours, at which the mixture was homogeneous. The CH^OH was evaporated and the aqueous solution was allowed to stir for an 10 additional three hours and then lyophilized to provide a green residue. The crude product was chromatographed on CHP20P, eluting with water (fractions 1-15) followed by a gradient created by the gradual addition of 3:1 acetonitrile:water to 15 a 500 ml reservoir of water, collecting 8-10ml fractions. Fractions 48-53 (Cut A, 150 mg) contained relatively pure material, whereas fractions 54-58 (Cut B, 273 mg) were contaminated with a UV absorbing material tentatively assigned 20 to be the l-methyl-2,4,6-collidinium cation. Cut B was dissolved in water, adjusted to pH 2, and passed through a column of AG 50W-X8 resin (Na+ form, 30 ml resin bed), eluting with water, to remove the contaminating cation. Fractions 25 containing the desired product were lyophilized and combined with Cut A from above. An aqueous solution of this material was made basic with 1 M NaOH and was purified by MPLC on a 20 cm height, 2.5 cm diameter column of CHP20P, eluted with water 30 (fractions 1-7) followed by gradient created by the gradual addition of 7:3 acetonitrile:water to a 500 ml reservoir of water, collecting 6-8 ml fractions. Fractions 46-58 gave 331.2 mg (71%) of title product as a pale yellow-white lyophilate. TLC Silica gel (6:3:1 n-C^OEicon NH3:H20) Rf=0.4 IR(KBr) 3446, 2967, 2924, 2860, 1629, 1175, 1072, 967 cm"1. 1H NMR (D20) (400 MHZ) 5.29 (t, 1H, J=7.3 Hz) .15 (m, 2H) 3.66 (d, 2H, J=7.7 Hz) 3.03 (d, 2H, J=ll, Hz) 1.9-2.2 (m, 10H) 1.72 (s, 3H) 1.66 (s, 3H) 1.59, 1.60 (two s, 3H each) ppm. 31P NMR (D20, NaOD) 635.1 (d, J=ll Hz) 12.9 (d, J=li Hz) ppm.

Mass Spec (FAB, + ions) m/e 460 (M+Na), 438 (M+H), 234, 187.

Anal Calcd for Cn_H01N0PP^Nao x 0.32 mol Ho0: 17 31 5 2 2 2 C, 46.08; H, 7.20; N, 3.16; P, 13.98 Found: C, 46.27; H, 7.53; N, 3.35; P, 13.58 Example 14 (E,E)-[[Ethoxy [2-[(3,7,ll-trimethyl-2,6,10-dodec-atrienyl)oxy]ethyl]phosphinyl]methyl]phosphonic acid, dimethyl ester A. (E,E)-[2-(3,7,ll-Trimethyl-2,6,10-dodecatrienyl)oxy]ethyl]phosphonic acid, diethyl ester (E,E)-Farnesol was purchased from Aldrich Chemical and further purified by preparative scale chromatography prior to use. < /< "7 »-■.

A solution of 3.00 g (13.5 mmol) of said farnesol and 2.70 ml (2.7 mmol, 0.2 eq.) of a 1M solution of tetra-n-butylammonium fluoride in THF in 25 ml of THF at room temperature under argon 5 was stirred for four hours over 4A molecular sieves. Then, 6.25 ml (40.5 mmol, 3 eq.) of diethyl vinylphosphonate was added and the reaction was allowed to stir for 70 minutes. After diluting with 75 ml of diethyl ether, the mixture was 10 filtered through Celite, washed with three 20 ml portions of water and 20 ml of brine, dried over MgSC>4 and evaporated to leave 5.62 g of crude mixture. Purification by flash chromatography on 500 g of silica gel, eluted with 4:6 ethyl 15 acetate:petroleum ether provided 2.483 g (48%) of title compound as a clear colorless oil.

TLC Silica gel (1:1 ethyl acetate:hexane) Rf=0.16 IR(CC14) 2979, 2927, 2915, 2862, 1444, 1389, 1249, 1096, 1056, 1032, 990, 960 cm"1. 1H NMR (CDC13) (270 MHz) 55.33 (t, 1H, J=6.9 Hz) .08 (m, 2H) 4.10 (m, 4H) 3.99 (d, 2H, J=6.9 Hz) 3.67 (dt, 2H, J=ll.l, 6.9 Hz) 1.9-2.2 (m, 10H) 1.67 (s, 6H) 1.59 (s, 6H) 1.32 (dt, 6H, J=l.l, 6.9 Hz) ppm.

Mass Spec (CI-CH4/N20, + ions) m/e 427 (M+C3H5), 415 (M+C2H5), 387 (M+H), 183. i § 7 f-. ~y .•-i =* i-i. « J \J B. (E,E)-[2-[(3,7,ll-Trimethyl-2,6,10-dodecatrieny1)oxy]ethyl]phosphonic acid, monoethyl ester A solution of 2.481 g (6.42 mmol) of Part A 5 phosphonate in 64 ml of ethanol and 64.2 ml (64.2 mmol, 10 eq.) of 1M NaOH at 60-70°C under nitrogen was stirred for 18 hours. The mixture was cooled and adjusted to ^pH 7 with 10% HCl. The ethanol was evaporated and the residue was acidified. The 10 aqueous mixture was extracted with four 150 ml portions of ethyl acetate. The combined organic phases were washed with 100 ml each of 50% brine and saturated brine, dried over MgSO^ and evaporated to yield 2.345 g (100%) of title 15 compound as a pale yellow oil.

TLC Silica gel (6:3:1 n-C3H?OH:con. NH3:H20) Rf=0.68, IR(CC14) 2978, 2972, 2926, 2916, 2870, 2859, 2450, 1669, 1445, 1379, 1237, 1202, 1090, 1049, 1001, 965, 802, 780, 771, 751, 740 cm"1.

XH NMR (CDC13) (270 MHz) 65.34 (t, 1H, J=6.9 Hz) .10 (m, 2H) 4.09 (quint, 2H, J=6.9 Hz) 3.99 (d, 2H, J=6.9 Hz) 3.68 (dt, 2H, J=9.5, 7.9 Hz) 1.9-2.2 (m, 10H) 1.67 (s, 6H) 1.60 (s, 6H) 1.33 (t, 3H, J=6.9 Hz) ppm.

Mass Spec (FAB, + ions) m/e 397 (M+K), 381 (M+Na), 359 (M+H), 331, 313, 155. <r, / t *7 ** * 3 0 C. (E,E)-[[Ethoxy[2-[(3,7,11-trimethyl-2,6,10-dodecatrienyl)oxy]ethyl]-phosphinyl]methyl]phosphonic acid, dimethyl ester A solution of 2.123 g (6.54 mmol) of crude Part B phosphonic acid in 20 ml of dry CHjC^ at room temperature under argon was treated with 2.50 ml (13.1 mmol, 2 eq.) of diethyl(trimethylsilyl)-amine. After two hours, the solvent was 10 evaporated and the residue was twice evaporated from benzene, then dried at high vacuum for 0.5 hours.

The residue was dissolved in 20 ml of dry and two drops of dimethylformamide (DMF) at 15 0°C under nitrogen and treated with 1.00 ml (11.8 mmol, 1.8 eq.) of oxalyl chloride. The mixture was allowed to warm to room temperature and stirred for 3 hours. The solvent was evaporated and the residue was twice evaporated from benzene, 20 then dried at high vacuum for 0.5 hour.

The anion solution was prepared by adding over five minutes a solution of 8.75 ml (14.1 mmol, 2.15 eq.) of 1. 6M n-butyllithium in hexanes to a solution of 1.55 ml (14.4 mmol, 2.2 eq.) of 25 dimethyl methylphosphonate in 15 ml of THF at -78°C under argon, followed by stirring for 0.5 hour.

A solution of the phosphonic acid chloride prepared above in 15 ml of THF at -78°C under argon was added as quickly as possible via cannula 30 to the anion solution. The resulting mixture was stirred at -78°C for 1.5 hours, then quenched with 0.75 ml (13.1 mmol, 2 eq.) of glacial acetic acid, «*»» 6 A l:'\ and allowed to stir for ten minutes. Approximately 3 ml of saturated ammonium chloride was added, and the cold bath was removed. Once at room temperature, 20 ml of H^O was added and the mixture was extracted with five 80 ml portions of CE^Cl^- The combined organic phases were washed with 100 ml each of 1:1 brine:water and then brine, dried over MgSO^ and evaporated to yield 2.773 g of crude product. Gross impurities were removed by flash chromatography on 300 g of Merck 9385 silica, eluted with 2:98 CHgOHrC^C^ to provide 913 mg (33%) of nearly pure material. A second flash chromatography on 100 g of Merck 9385 silica, eluted with 2:8 ethyl acetate:acetone afforded 414.2 mg (15%) of title compound as a clear, colorless oil.

TLC Silica gel (5:95 CH3OH:CH2C12) Rf=0.17 IR(CC14) 2955, 2918, 2856, 1448, 1377, 1369, 1257, 1240, 1185, 1167, 1064, 1037, 995, 957, 844 cm"1. 1H NMR (CDC13) (270 MHz) 55 .33 t, 1H, J=6.9 Hz) .10 m, 2H) 4 .16 quint, 2H, J=7.2 Hz) 4 .01 d, 2H, J=6.9 Hz) 3 .80 d, 6H, J=ll.l Hz) 3 .6-3 8 (m, 2H) 2 .53 dd, 2H, J=20.6, 16.9 Hz) 2 .26 m, 2H) 1 .9-2 2 (in, 8H) 1 .67 s, 6H) 1 .60 s, 6H) 1 .34 t, 3H, J=7.2 Hz) ppm.

Mass Spec (CI-CH4/N20, + ions) m/e 493 (M+C2H5), 465 (M+H), 261. -104' Example 15 (E,E)- [ [Hydroxy[2-[(3,7, ll-trimethyl-2,6,10-dodecatrienyl)oxy]ethyl]phosphinyl]methyl]- phosphonic acid, trisodium salt To a solution of 343.3 mg (0.79 mmol) of Example 14 triester and 315 pi (2.37 mmol, 3 eq. ) of 2,4,6-collidine in 5 ml of dry at room temperature under nitrogen in the dark was added 630 pi (4.75 mmol, 6 eq.) of bromotrimethyl-10 silane. After 6 hours, additional 105 pi (0.79 mmol, 1 eq.) of 2,4,6-collidine and 210 pi (1.58 mmol, 2 eq.) of bromotrimethylsilane were added and the reaction was allowed to stir overnight. The solvent was evaporated and the residue was 15 twice evaporated from benzene and dried at high vacuum for 0.5 hour. Then 6.35 ml (6.35 mmol, 8 eq.) of 1M NaOH was added and the solution was lyophilized. Preliminary purification was by HP-20 chromatography on a 16cm height x 2.5cm 20 diameter column packed with water and eluted with 200 ml of water followed by a gradient created by the gradual addition of 400 ml acetonitrile to 400 ml of ^0. Approximately 8-10 ml fractions were collected every 1.5 minutes. Fractions 24-53 were 25 combined, evaporated and lyophilized to afford 220.2 mg (54%) of a fluffy white lyophilate, which however, showed impurities on NMR analysis. The lyophilate was dissolved in water, adjusted to pH 12 with 1M NaOH, and was chromatographed on a 12 30 cm height x 2.5 cm diameter column of CHP20P resin packed in water. The column was eluted with a gradient created by the gradual addition of 300 mL of acetonitrile into 300 mL of 2:98 acetonitrile: o water. Approximately 8-10 mL fractions were collected every 1.5 minutes. Fractions 22-26 were combined, evaporated, lyophilized and pump-dried for 6 hours to provide 145.7 mg (42%) of title product as a flocculent white lyophilate.

TLC Silica gel (5:4:1 n-C3H7OH:Con. NH3:H20) Rf=0.36. IR(KBr) 3400 (br), 2967, 2923, 2859, 1663, 1640, 1631, 1448, 1381, 1167, 1128, 1099, 1083, 978, 807 cm-1.

XH NMR (D20) (400 MHz) 65 .33 t, 1H, J=7. 1 Hz) .14 t, 1H, II >-) 1 Hz) .12 t, 1H, J=7. 1 Hz) 4 .01 d, 2H, J=7. 1 Hz) 3 .70 m, 2H) 2 .0-2 1 (m, 8H) 1 .96 t, 2H, J=7. 1 Hz ) 1 .87 t, 2H, II CD .3 Hz) 1 .64 s, 3H) 1 .62 s, 3H) 1 .56 s, 3H) ppm. 31P-NMR (D2O 638.7 (d, J=5.86 Hz) 14.6 (d, J=5.86 Hz) ppm.

Mass Spec (FAB, + ions) m/e 497 (M+Na), 475 (M+H), 453 (M+2H-Na).

Anal Calcd for C18H3106P2Na3«1.05 H20 (MW 493.33): C, 43.82; H, 6.76; P, 12.56 Found: C, 43.59; H, 6.72; P, 12.52 v !■ 7 £ L. ^ ~'i J \) -106-Example 16 (E)-[[[[(7,ll-Dimethyl-6,10-dodecadien-2-ynyl )oxy]-methyl](1-me thylethoxy )phosphinyl ] methyl ] phosphonic acid, dimethyl ester A. (E)-2,6-Dimethyl-2,6-undecadien-10-yne A solution of 4.60 ml (27.0 mmol, 1.05 equiv.) of 2,2,6,6-tetramethylpiperidine in 55 ml of tetrahydrofuran at 0°C under argon was treated with 17.0 ml (27.0 mmol, 1.05 equiv.) of 1.6 M 10 n-butyllithium in hexanes and the mixture was stirred at 0°C for 0.5 hours. After cooling to -78°C a solution of 5.75 ml (25.7 mmol) of (E)-geranyl acetone (obtained from FLUKA) and used in 15 ml of tetrahydrofuran was added over 15 minutes. 15 The reaction was stirred for one hour, then 4.10 ml (28.3 mmol, 1.1 equiv.) of diethyl chlorophosphate was added. The mixture was allowed to warm to room temperature over two hours to provide a solution of the enol phosphate.

A second solution of the lithium piperidide was prepared by treating a solution of 9.8 ml (57.8 mmol, 2.25 equiv.) of 2,2,6,6-tetramethylpiperidine in 120 ml of tetrahydrofuran at 0°C under argon with 36.2 ml (157.8 mmol, 2.25 eq.) of 25 1.6M n-butyllithium in hexanes, stirring at 0°C for 0.5 hour, then cooling to -78°C. The enol phosphate solution was added to the anion solution dropwise over 65 minutes and the resulting mixture was allowed to warm to room temperature over two hours. 30 After quenching the reaction with 1^0, the acetylene was extracted with three 250 ml portions of hexane. The combined organic phase was washed with 100 ml of 1 M HCl, 100 ml of H20, 100 ml of saturated / iv\ .1 /, x p; r \J U 0 NaHC03, and 100 ml of brine, dried over MgS04 and evaporated to obtain 6.123 g of crude product as a dark orange oil. Purification by flash chromatography on 600 g Merck 9385 silica eluted with hexane 5 provided 2.201 g (49%) of title acetylene as a clear, colorless oil.

TLC Silica gel (hexane) R^=0.23 IR(CC14) 3314, 2968, 2915, 2856, 2119, 1448, 1433, 1383, 1377, 805, 797, 789, 779, 754, 634 cm"1. 10 1H-NMR (CDC13) (270 MHz) 65.18 (t, 1H, J=5.6 Hz) .10 (t, 1H, J=6.1 Hz) 2.20 (rti, 4H) 2.0-2.1 (m, 4H) 1.92 (t, 1H, J=2.3 Hz) 1.68 (s, 3H) 1.62 (s, 3H) 1.61 (s, 3H) ppm.

Mass Spec (CI-NH3, + ions) m/e 353 (2M+H), 283, 20 177 (M+H).

B. (E)-7,ll-Dimethyl-6,10-dodecadien-2- yn-l-ol A solution of 2.162 g (12.3 mmol) of Part A 25 terminal acetylene in 40 ml of tetrahydrofuran at -78°C under argon was treated with 8.45 ml (13.5 mmol, 1.1 equiv.) of 1.6 M n-butyllithium in hexanes. After one hour, 554 mg (18.5 mmol, 1.5 equiv.) of dry paraformaldehyde was gradually 30 added, and the reaction was allowed to warm slowly to room temperature and was stirred for four hours. Saturated NHjCl was added and the mixture was extracted with 200 ml of diethyl ether. The 1 v r. /. 4 ,W f *~T t / $ j> organic phase was washed with 20 ml of H20, and 20 ml of brine, dried over MgSO^ and evaporated to give 2.561 g of a pale yellow oil. Purification by flash chromatography on 200 g of silica gel 5 eluted with 1:9 ethyl acetate:hexane yielded 2.172 g (86%) of title alcohol as a clear, colorless oil. TLC Silica gel (2:8 ethyl acetate:hexane) R^=0.36. IR(CC14) 3622, 3520, 2967, 2917, 2873, 2857, 2284, 2221, 1660, 1447, 1381, 1134, 1009, 805, 794, 777, 10 756, 738 cm"1.

XH NMR (CDClg) (270 MHz) 65.16 (m, 1H) .10 (m, 1H) 4.24 (br s, 2H) 2.30 (br t, 1H) 2.22 (m, 4H) 1.9-2.1 (m, 4H) 1.68 (s, 3H) 1.62 (s, 3H) 1.60 (s, 3H) ppm.

Mass Spec. (CI-NH3, + ions) m/e 224 (M+NH4).

C- (E)—[[(7,ll-Dimethyl-6,10-dodecadien-2-ynyl)oxy]methyl]phosphonic acid, bis(1-methylethyl) ester A solution of 603 mg (2.91 mmol) of Part B propargylic alcohol in 10 ml of tetrahydrofuran at -78°C under argon was treated with 1.90 ml (3.06 mmol, 1.05 equiv.) of 1.6 M n-butyllithium in 30 hexanes. The reaction was stirred 15 minutes at -78°C, 15 minutes at 0°C, then recooled to -78°C. A solution of 1.004 g (3.06 mmol, 1.05 equiv.) of Example 1 Part B triflate in 3 ml of J \J \J % tetrahydrofuran was added in rapid drops. The solution was stirred 1.5 hours at -78°C and 4.5 hours at 0°c. The reaction was quenched with saturated NH^Cl and extracted with 100 ml of 5 diethyl ether. The organic phase was washed with three 20 ml portions of H20, and 20 ml brine, dried over MgSO^ and evaporated to yield 1.087 g of crude product. Purification by flash chromatography on 70 g of silica gel, eluted 10 with 3:7 ethyl acetate:hexane provided 964 mg (87%) of title phosphonate as a clear, pale yellow oil.

TLC Silica gel (2:8 ethyl acetate:hexane) R£=0.07 IR(CC14) 2979, 2928, 2854, 1465, 1450, 1385, 1375, 15 1357, 1256, 1242, 1178, 1136, 1105, 991, 937, 901, 889, 795, 788, 777, 772, 754 cm"1. 1H NMR (CDC13) (270 MHz) 55.17 (br t, 1H, J=6.2 Hz) .09 (br t, 1H J=6.2 Hz) 20 4.76 (m, 2H) 4.25 (s, 2H) 3.80 (d, 2H, J=8.8 Hz) 2.22 (m, 4H) 1.9-2.1 (m, 4H) 1.68 (d, 3H, J=1.2 Hz) 1.62 (d, 3H, J=1.2 Hz) 1.60 (s, 3H) 1.34 (d, 12H, J=6.5 Hz) ppm.

Mass Spec (CI-NH3, + ions) m/e 402 (M+NH^), 385 30 (M+H). 24 4 3 6 3 D. (E)-[[(7,ll-Dimethyl-6,10-dodecadien-2-ynyl)oxy]methyl]phosphonic acid, mono(1-methylethyl) ester A mixture of 951.3 mg of Part C diester, 25 5 ml of isopropanol and 25 ml (25 mmol, 10.1 equiv.) of 1 M KOH under nitrogen was stirred three days at 85-90°C, then cooled. The solution was neutralized to ^pH7 with 10% HCl and the isopropanol was evaporated. The remaining aqueous 10 solution was diluted with ethyl acetate, acidified to pH 1 and separated. The aqueous phase was extracted with three 50 ml portions of ethyl acetate. The organic layers were combined and washed with 30 ml of brine, dried over MgSO^ and 15 evaporated to obtain 827.0 mg ("98%") of title phosphonic acid.

TLC Silica gel (8:1:1 n-C^HyOH:con. NH^^O) R^=0.57 IR(CC14) 2979, 2922, 2918, 2853, 1385, 1237, 1221, 1202, 1179, 1135, 1100, 1013 cm"1. 1H NMR (CDC13) (270 MHz) 510.9-11.1 (br, 1H) .15 (br, 1H) .09 (br t, 1H) 4.76 (m, 1H) 4.26 (s, 2H) 3.83 (d, 2H, J=8.8 Hz) 2.23 (ra, 4H) 1.9-2.1 (m, 4H) 1.68 (s, 3E) 1.61 (s, 3H) 1.60 (s, 3H) 1.35 (d, 6H, J=6.45 Hz) ppm.

Mass Spec (FAB,-ions) m/e 341 (M-H), 299 6 s i v/ E- (E)—[[[[(7,ll-dimethyl-6,10-dodeca-dien-2-ynyl)oxy]methyl](1-methylethoxy)-phosphinyl]methyl]phosphonic acid, dimethyl ester A solution of 798.3 mg (2.34 mmol) of Part D phosphonic acid in 12 ml of at room temperature under argon was treated with 890 pi (4.68 mmol, 2 equiv.) of N,N-diethyl(trimethylsilyl)-10 amine, stirred 1.5 hours and evaporated. The residue was twice evaporated from benzene and pump-dried for 30 minutes.

The residue was dissolved in 12 ml of CH2C12 and one drop of dimethylformamide at 0°C 15 under nitrogen, treated with 370 pi (4.21 mmol, 1.8 equiv.) of oxalyl chloride, and stirred at room temperature for 2.5 hours. The solvent was evaporated, and the residue was twice evaporated from benzene and pump-dried for 30 minutes. 20 The anion solution was prepared by treating 560 pi (5.15 mmol, 2.2 equiv.) of dimethyl methylphosphonate in 12 ml of tetrahydrofuran at -78°C under argon with 3.5 ml (5.03 mmol, 2.15 equiv.) of 1.6 M n-butyllithium in hexanes.

After 0.5 hours a solution of the phosphonic acid chloride prepared above in 2 ml of tetrahydrofuran was added. The mixture was stirred for 1.5 hours, then quenched with 300 pi (5.15 mmol, 2 equiv.) of acetic acid in tetrahydrofuran and stirred for ten 30 minutes. Then, 5 ml of NH^Cl was added and the product was extracted with four 30 ml portions of CI^C^. The combined organic phases were washed with brine, dried over MgS04 and evaporated to 9 4 3 6 obtain 1.193 g of crude product. Purification required two chromatographies. The first was run on 70 g of silica gel, eluted with 2.5:97.5 CH3OH:CH2Cl2 to provide 140.6 mg of pure title 5 compound and 509.3 mg of impure fractions. The impure fractions were rechromatographed on 50 g of silica gel, eluted with 1.8:98.2 CH3OH:CH2Cl2 to provide 456.1 mg of pure title compound. The combined yield of 606.7 mg (58%) of 10 title compound was obtained as a yellow oil. TLC Silica gel (2:98 CH30H:CH2C12) Rf=0.09 IR(CC14) 2977, 2954, 2923, 2853, 1257, 1230, 1179, 1166, 1094, 1063, 1036, 993, 841, 818 cm"1.

XH NMR (CDC13) (270 MHz) 55 .08 br t, 1H, J=5.9 Hz) .02 br t, 1H, J=6 .5 Hz) 4 .74 m, 1H) 4 .21 d, 1H, J=15.5 Hz) 4 .14 d, 1H, J=15.5 Hz) 3 .85 d, 2H, J=7.6 Hz) 3 .75 d, 3H, J=12 Hz) 3 .72 d, 3H, J=ll Hz) 2 .43 m, 2H) 2 .16 m, 4H) 1 .9-2 0 (m, 4H) 1 .60 s, 3H) 1 .55 s, 3H) 1 .52 s, 3H) 1 .30 d, 3H, J=5.9 Hz) 1 .28 d, 3H, J=5.9 Hz) ppm.

Mass Spec (CI-CH4/N20, + ions) m/e 489 (M+C3H5), 477 (M+C2H5), 449 (M+H), 435, 407, 219. r. v / T E * & & f) L Example 17 (E)-[[[[(7,ll-dimethyl-6,10-dodecadien-2-ynyl)-oxy]methyl]hydroxyphosphinyl]methyl]phosphonic acid, tripotassium salt A solution of 598.3 mg (1.33 mmol) of Example 16 triester in 8 ml of CH2Cl2 at room temperature under argon was treated with 530 pi (3.99 mmol, 3 equiv.) of 2,4,6-collidine and 1.05 ml (7.98 nunol, 6 equiv.) of bromotrimethylsilane 10 and stirred for 24 hours. Additional 90 pi (67 mmol, 0.5 equiv.) of 2,4,6-collidine and 175 pi (1.33 mmol, 1 equiv.) of bromotrimethylsilane were added. The reaction was stirred for three hours and evaporated. The residue was dissolved in 18.6 15 ml (18.6 mmol, 14 equiv.) of 1 M KOH and 10 ml of ^0, and lyophilized. The lyophilate was dissolved in 5 ml of E20 and purified on a 2.5 cm diameter x 18 cm height column of HP-20 resin packed in water. The column was eluted with 100 20 ml of E^O followed by a gradient created by the gradual addition of 300 ml CH^CN into 300 ml of H20. Approximately 10 ml fractions were collected every 1.8 minutes. Fractions 20-27 were combined, evaporated and lyophilized and dried under vacuum 25 to obtain 472.4 mg (72%) title salt as a white lyophilate.

TLC Silica gel (5:4:1 n-C^OH: con. NH3:H20) Rf=0.63. IR (KBr) 3424, 2966, 2924, 2858, 1634, 1175, 1130, 1066, 970 cm"1.

XH NMR (D20) (400 MHz) 55.20 (t, 1H, J=6.8 Hz) .14 (t, 1H, J=6.2 Hz) 4.19 (s, 2H) r% L /, 1 R "? s H ^ \j yj ^ 3.67 (d, 2H, J=6.2 Hz) 2.15-2.25 (m, 4H) 2.06 (m, 2H) 1.99 (m, 2H) 1.91 (t, 2H, J=18.1 Hz) 1.63 (s, 3H) 1.58 (s, 3H) I.56 (s, 3H) ppm. 31P NMR (D20) 632.47 (d, J=8.06 Hz) II.72 (d, J=8.06 Hz) ppm.

Mass Spec. (FAB, + ions) m/e 531 (M+K), 493 (M+H), 455 (M+2H-K), 437 (M+2H-K-H20) Anal Calcd for C^,HocO,P„•K_•1.55 mol Ho0: 16 25 6 2 3 2 C, 36.92; H, 5.44; P, 11.90 Found: C, 36.58; H, 5.16; P, 12.22 Example 18 (E,E)-[[[[(3,7-Dimethyl-2,6-dodecadienyl)oxy]-20 methyl](l-methylethoxy)phosphinyl]methyl]- phosphonic acid, dimethyl ester A. 2,2-Dimethylpropanoic acid, (E,E)-3,7,ll-trimethyl-2,6,10-dodecatrienyl ester To a stirred solution of 4.08 g (18.3 mmol) of (E,E)-farnesol in 50 ml of CH2C12 at 0°C under argon was added 206 mg (1.69 mmol) of 4-dimethyl-aminopyridine and 3.8 ml (27.4 mmol) of triethylamine, followed by the dropwise addition 30 of 2.7 ml (22.0 mmol) of trimethylacetyl chloride over 15 minutes. The suspension was stirred for 1 hour at 0°C followed by 45 minutes at room 9 A 4 3 6 3 luv v * temperature, and partitioned between CH2C12 and water. The organic layer was washed with 10% HCl, saturated NaHC03, and brine, dried (MgSO^), and evaporated to provide 5.9 g of a crude liquid.

Flash chromatography on 200 g of silica gel, packed and eluted with 1.5:98.5 ethyl acetate:hexane gave 5.53 g (98%) of pure title pivalate as a colorless liquid.

TLC Silica gel (5:95 ethyl acetate:hexane) R^=0.39, 10 IR (CC14) 2970, 2930, 2875, 2857, 1727, 1480, 1456, 1281, 1152 cm"1.

XH NMR (CDC13) 65.35 (td, 1H, J=7, 1 Hz) .11 (m, 2H) 4.59 (d, 2H, J=7 Hz) 2.10 (m, 8H) 1.73 (s, 3H) 1.70 (s, 3H) 1.62 (s, 6H) 1.22 (s, 9H) ppm.

Mass Spec (EI) m/e 306 (M), 204, 136, 69.

B. 2,2-Dimethylpropanoic acid, (E,E)-10-bromo-ll~hydroxy-3,7,ll-trimethyl-2,6- dodecadienyl ester To a solution of 8.18 g (26.7 mmol) of Part A pivalate in 100 ml of 7:3 t-butanol:water at 0°C was added 4.74 g (26.7 mmol) of recrystallized N-bromosuccinimide, portionwise over 30 minutes. 30 After 135 minutes at 0°C, the reaction mixture was diluted with 400 ml of diethyl ether and washed with water, 2 M K2C03 (two portions), water and brine. The organic layer was dried (MgSO^) and ,+k ^ / *7 r* v t\ u h Ci I » o - ■ evaporated to provide 10.9 g of a pale yellow liquid. Flash chromatography on 400 g of silica gel eluted with 10:90 ethyl acetate:hexane gave 6.76 g (63%) of pure title compound as a colorless 5 liquid.

TLC Silica gel (20:80 ethyl acetate:hexane) R£=0.31 XH NMR (CDC13) 65.32 (td, 1H, J=7, 1 Hz) .20 (t, 2H, J=7 Hz) 4.57 (d, 2H, J=7 Hz) 3.95 (dd, 1H, J=ll.2, 1.7 Hz) 2.30 (m, 2H) 1.90-2.20 (m, 6H) 1.80 (m, 1H) 1.70 (s, 6H) 1.60 (s, 6H) 1.339, 1.343 (two s, 6H total) 1.19 (s, 9H) ppm.

IR (CC14) 3610, 3580, 2978, 2958, 2874, 1726, 20 1282, 1152 cm"1.

Mass Spec (CI-NH3, + ions) m/e 420, 422 (M+NH^), 403, 405 (M+H), 318, 320, 301, 303, 283, 285.

C. 2,2-Dimethylpropanoic acid, (E,E)-10,11-25 epoxy-3,7,ll-trimethyl-2,6-dodecadienyl ester To a stirred solution of 6.65 g (16.5 mmol) of Part B compound in 50 ml of tetrahydrofuran under argon at -78°C was added 17 ml (17 mmol) of 30 1 M sodium bis(trimethylsilylamide) in tetrahydrofuran over 20 minutes. After 30 minutes, the reaction was allowed to warm to 0°C for 40 minutes, when it was diluted with C^C^ and O /, /• X p. X r '*v a'.' \ J ; IVXI I * ^ «- quenched with water. The organic layer was washed with water and brine, dried (MgSO^) and evaporated to provide 5.30 g (99%) of the title epoxide as a colorless liquid.

TLC Silica gel (20:80 ethyl acetate:hexane) R^=0.48 IR (CC1.) 2963, 2929, 2874, 1727, 1479, 1281, 1151 -1 * cm LH NMR (CDC13 ) 55.33 (td, 1H, J=7,1 Hz) 5.11 (td, 1H, J=7,1 Hz) 4.57 (d, 2H, J=7 Hz) 2.69 (t, 1H, J=6 Hz) 2.10 (m, 6H) 1.70 (s, 3H) 1.62 (s, 3H) 1.50-1.70 (m, 2H) 1.26, 1.30 (two s, 6H) 1.20 (s, 9H) ppm.

Mass Spec (CI-NH^, + ions) m/e 340 (M+NH^), 323 20 (M+H), 221.

D. 2,2-Dimethylpropanoic acid, (E,E)-3,7-dimethyl-10-oxo-2,6-decadienyl ester To a stirred solution of 4.22 g (13.1 mmol) 25 of the Part C epoxide in 50 ml of tetrahydrofuran at 0°C was added 3.1 g (13.6 mmol) of periodic acid, portionwise over 10 minutes. After 40 minutes, the reaction was diluted with diethyl ether, washed with water, saturated NaHC03, and brine, 30 dried (MgSO^) and evaporated to provide 3.88 g of crude aldehyde. The crude product was purified by flash chromatography on 300 g of silica gel, packed in 3:97 and eluted with 5:95 ethyl acetate: n / / MW i « I :\ hexane to afford 2.59 g (70%) of pure title aldehyde as a colorless oil.

TLC Silica gel (10:90 ethyl acetate:hexane) Rf=0.26.

IR (CC1.) 2972, 2931, 2917, 1728, 1479, 1291, 1151 cm"1.

NMR (CDC13) 69.74 (t, 1H, J=1.7 H2) .33 (td, 1H, J=7,1 HZ) 5.13 (td, 1H, 3-1,1 HZ) 4.56 (d, 2H, J=6.5 Hz) 2.50 (td, 2H, 3=1,1.1 HZ) 2.31 (t, 2H, J=7 Hz) 2.07 (m, 4H) 1.69 (s, 3H) 1.62 (s, 3H) 1.19 (s, 9H) ppm.

Mass Spec (CI-NH3, + ions) m/e 298 (M+NH^).

E. 2,2-Dimethylpropanoic acid, (E,E)-3,7- dimethyl-10-[[(4-methylphenyl)sulfonyl]- oxy]-2,6-dodecadienyl ester To a solution of 1.01 g (3.59 mmol) of Part D aldehyde in 12 ml of tetrahydrofuran at -78°C 25 was added 1.8 ml (3.60 mmol) of 2 M ethylmagnesium bromide in diethyl ether. After 30 minutes, the reaction was allowed to warm to 0°C for 40 minutes. A solution of 753 mg (3.95 mmol) of p-toluenesulphonyl chloride in 5 ml of tetrahydrofuran was added 30 rapidly via cannula, and the reaction was allowed to stir for 210 minutes at 0°C. The mixture was diluted with diethyl ether, washed successively with 1 M HCl, water, saturated NaHCO^ and brine, 24 4363 dried (MgSO^) and evaporated to provide 1.72 g of crude tosylate. Flash chromatography on 180 g of silica gel, eluted with 5:95 ethyl acetate:hexane gave 1.29 g (78%) of pure title tosylate as a 5 colorless oil.

TLC Silica gel (20:80 ethyl acetate:hexane) R^=0.19 IR (CC14) 2972, 2958, 2935, 1726, 1370, 1344, 1281, 1188, 1177, 1151, 915 cm"1.

XH NMR (CDC13) 67.79 (d, 2H, J=8 Hz) 7.32 (d, 2H, J=8 Hz) .32 (t, 1H, 3=1 Hz) .02 (t, 1H, 3=1 Hz) 4.57 (d, 2H, 3=1 Hz) 4.50 (quint, 1H, 3=6 Hz) 2.44 (s, 3H) 2.04 (m, 4H) 1.91 (m, 2H) 1.69 (s, 3H) 1.52 (s, 3H) 1.50-1.80 (m, 4H) 1.19 (s, 9H) 0.83 (t, 3H, 3=1 Hz) ppm.

Mass Spec (CI-NH^, + ions) m/e 482 (m+NH^).

F. (E,E)-3,7-Dimethyl-2,6-dodecadien-l-ol To a stirred solution of 1.27 g (2.75 nunol) of Part E tosylate in 15 ml of tetrahydrofuran at 0°C under argon was added 17 ml (17 nunol) of 1 M 30 LiB(C2H5)3H in tetrahydrofuran over 30 minutes.

The mixture was allowed to warro to room temperature for 3 hours, then cooled to -15°C and the excess hydride was slowly quenched with 2 4 4 3 6 3 water. The mixture was treated with 8.5 ml of 10% NaOH, followed by the cautious, dropwise addition of 7 ml of 30% hydrogen peroxide. After 1 hour, the reaction was allowed to warm to room 5 temperature for 2 hours and then diluted with 300 ml of 1:1 diethyl ether:hexane. The organic layer was washed with water (four-100 ml portions) and brine, dried (MgS04), and evaporated to provide 596 mg of crude alcohol. Flash chromatography on 10 65 g of silica gel eluted with 10:90 ethyl acetate:hexane gave 553 mg (95%) of pure title alcohol as a colorless oil.

TLC Silica gel (20:80 ethyl acetate:hexane) R^=0.24, IR (CC14) 3622, 3400 (br), 2959, 2929, 2858, 1666, 15 1456, 1382, 1175, 999 cm"1. 1H NMR (CDC13) 65.41 (td, 1H, J=7, 1 Hz) .09 (td, 1H, J=7, 1 Hz) 4.13 (d, 2H, J=7 Hz) 2.07 (m, 4H) 1.95 (t, 2H, J=7 HZ) 1.76 (br s, 1H) 1.67 (s, 3H) 1.58 (s, 3H) 1.10-1.50 (m, 6H) 0.88 (t, 3H, J=7 Hz) ppm.

Mass Spec (EI) m/e 210 (M), 192 (M-H20).

G. (E,E)-[[(3,7-Dimethyl-2,6-dodecadienyl)-30 oxy]methyl]phosphonic acid, bis(1-methyl- ethyl) ester To a stirred solution of 776.8 mg (3.69 mmol) of Part F alcohol in 8 ml of THF at -78°C 24 4 3 6 under argon was added 2.3 ml (3.69 mmol) of 1.6 M n-butyllithium in hexane over 10 minutes. After 1 hour at -78°C, a solution of 1.21 g (3.69 mmol) of Example 1 Part B triflate in 6 ml of THF was added 5 via cannula. The reaction was maintained at -78°C for 30 minutes, and then allowed to warm to 0°C for 2 hours. After quenching with saturated NHdCl, the mixture was extracted with diethyl ether and the organic layer was washed with water 10 and brine, dried (MgSO^) and evaporated to provide 1.41 g of a crude yellow liquid. Flash chromatography on 70 g of silica gel packed in 20:80 and eluted with 30:70 ethyl acetate:hexane provided 1.24 g (86%) of pure title ether as a 15 colorless oil.

TLC Silica gel (50:50 ethyl acetate:hexane) R^=0.30. IR(CC14) 2978, 2953, 2929, 2867, 1456, 1380, 1257, 1107, 990 cm"1. 1 H NMR (CDC13) 65.32 (td, 1H, 3=1, 1 Hz) .09 (t, 1H, J=6.5 Hz) 4.77 (m, 2H) 4.12 (d, 2H, J=7 Hz) 3.68 (d, 2H, J=8.2 Hz) 2.07 (m, 4H) 1.95 (t, 2H, 3=1 Hz) 1.68 (s, 3H) 1.58 (s, 3H) 1.35, 1.36 (two d, 12H total, J=6 Hz) 30 1.10-1.50 (m, 6H) 0.88 (t, 3H, 3=1 Hz) ppm.

Mass Spec (CI-CH4/N20, + ions) m/e 429 (M+C^), 417 (M+C2H5), 389 (M+H), 197. 0 L h 3 0 H. (E,E)- [ [(3,7-Dimethyl-2,6-doaecadienyl)-oxy]methyl]phosphonic acid, mono(l-methylethyl) ester A solution of 749.2 mg (1.93 mmol) of Part 5 G ether in 12 ml of 2-propanol was treated with 12 ml of 1 M KOH and was heated to reflux under nitrogen for 48 hours. The 2-propanol was evaporated and the aqueous solution was stirred with dichloromethane and acidified with 10% KC1. 10 The organic layer was washed with water and brine, dried (MgSO^) and evaporated to provide 660 mg (99%) of a colorless oil.

TLC Silica gel (8:1:1 n-C-H-OH:con. NH^H-O) Rf=0.48 1H NMR (CDCU) 55.31 (t, 1H, J=7 HZ) .09 (t, 1H, J=6.5 Hz) 4.77 (m, 1H) 4.12 (d, 2H, J=7 Hz) 3.71 (d, 2H, J=8.2 Hz) 2.07 (m, 4H) I.95 (t, 2H, J=7 Hz) 1.67 (s, 3H) 1.58 (s, 3H) 1.33 (d, 6H, J=6 Hz) 1.10-1.50 (m, 6H) 0.88 (t, 3H, J=7 Hz) ppm.

I. (E,E)-[[[[(3,7-Dimethyl-2,6-dodeca-dienyl)oxy]methyl](1-methylethoxy)-30 phosphinyl]methyl]phosphonic acid, dimethyl ester 2 h 4 3 f; 3 Cii. ( ! v > J To a stirred solution of 643.8 mg (1.35 mmol) of Part G ether in 7 ml of CK2Cl2 under argon was added 0.72 ml (3.80 mmol) of diethyl-(trimethylsilyl)amine and the reaction was allowed 5 to stir for 1.5 hours at room temperature. The solvent was evaporated, the remainder was dissolved in benzene, the solution was concentrated and the residual oil was pumped at high vacuum. To the residue in 7 ml of CH2C12 10 containing a drop of dimethyl formamide at 0°C under nitrogen was added 0.33 ml (3.80 mmol) of oxalyl chloride over 10 minutes. After 30 minutes at 0°C, the mixture was allowed to warm to room temperature for 45 minutes. The solvent was 15 evaporated, and the remainder was dissolved in benzene, the solution was evaporated and the residual orange oil was pumped at high vacuum.

In a separate flask, to 0.44 ml (4.07 mmol) of dimethyl methylphosphonate in 15 ml of THF at 20 -78°C under argon was added 2.5 mi (4.00 mmol) of 1.6 M n-butyllithium in hexane over 10 minutes. After 30 minutes, a solution of the above acid chloride in 6 ml of THF was added over 15 minutes. The reaction was allowed to stir for 1.5 25 hours at -78°C and was quenched with saturated NH^Cl. The mixture was diluted with CH2C12 and water, and the aqueous layer was acidified with 10% HCl. The organic layer was separated, the aqueous layer was re-extracted with CYL^<Z±^, and 30 the combined organic layers were washed with brine, dried (MgSO^), and evaporated to provide 821 mg of a yellow oil. The crude product was purified by flash chromatography on 70 g of silica 24 4 3 v 3 gel eluted with 2:98 CH30H:CH2C12 to provide 693.6 mg (83%) of pure title compund as a colorless oil. TLC Silica gel (5:95 CH3OH:CH2C12) Rf=0.23. IR (CC14) 2956, 2928, 2872, 1451, 1385, 1256., 5 1229, 1063, 1036, 992, 841 cm"1. 1H NMR (CDC13) 65.32 (td, 1H, J=7,1 Hz) .08 (td, 1H, J=7,1 Hz) 4.80 (m, 1H) 4.12 (d, 2H, J=7 Hz) 3.80, 3.82 (two d, 6H total, J=ll Hz) 3.70-3.90 (m, 2H) 2.40 (m, 2H) 2.07 (m, 4H) 1.95 (t, 2H, J=7 Hz) 1.68 (s, 3H) 1.58 (s, 3H) 1.34, 1.37 (two d, 6H total, J=6.5 Hz) 1.10-1.50 (m, 6H) 0.88 (t, 3H, J=7 Hz) ppm.

Mass Spec (CI-NH3, + ions) m/e 470 (M+NH^), 453 (M+H), 261.

Example 19 (E,E)-[[[f(3,7-Dimethyl-2,6-dodecadienyl)oxv]-methyl]hydroxyphosphinyl]methyl]phosphonic acid, trisodium salt A solution of Example 18 triester (653.3 mg, 1.44 mmol) in 7 ml of CH2C12 at room 30 temperature under argon was treated with 0.60 ml (4.54 mmol) of 2,4,6-collidine followed by 1.2 ml (9.09 mmol) of bromotrimethylsilane. After 23 hours, the solution was concentrated, the residue ■" 7 * ' l 4\ ■ % y ■ i iktt i was dissolved in benzene, the solvent was evaporated and the remainder was dried under vacuum. After dissolution in 8 ml of 1 M NaOH, the aqueous mixture was lyophilized. The crude 5 product was purified by MPLC on a 22 cm tall, 2.5 cm diameter column of CHP20P gel, eluted with water (fractions 1-12), followed by a gradient created by the gradual addition on 500 ml of 80:20 acetonitrile:water to 500 ml of water, collecting 10 approximately 8 ml fractions. Fractions 37-47 were combined, the acetonitrile was evaporated, the aqueous solution was lyophilized and the resulting powder dried under vacuum to provide 605.7 mg (92%) of title salt as a white, amorphous 15 solid.

TLC Silica gel (5:4:1 n-C3H7OH:con NH3:H20) Rf=0.4. IR (KBr) 3500 (br), 2957, 2872, 2858, 1664, 1456, 1381, 1192, 1149, 1135, 1104, 1081, 1051, 976, 800, 765 cm"1.

XH NMR (D20) 65.35 (t, 1H, J=7 Hz) .12 (t, 1H, J=7 Hz) 4.07 (d, 2H, J=7 Hz) 3.58 (d, 2H, J=6.5 Hz) 1.80-2.20 (m, 8H) 1.65 (s, 3H) 1.54 (s, 3H) 1.33 (quint, 2H, J=7 Hz) 1.20 (m, 4H) 0.80 (t, 3H, J=7 Hz) ppm. 24 4 3 5 31P NMR (D20) 632.3 (d, J=10.3 HZ) 12.8 (d, J=10.3 Hz) ppm.

Mass Spec (FAB, + ions) m/e 449 (M+H), 427 5 (M+2H-Na), 405 (M+3H-2Na) Anal Calcd for C16H29P206Na3 x 0.55 mol (2.16%) H20: C, 41.94; H, 6.62; P, 13.52 Found: C, 42.27; H. 7.02; P, 13.87 Example 20 (E,E)-[[(1-Methylethoxy)[[(3,7,ll-trimethyl-2,6-dodecadienyl)oxy]methyl]phosphinyl]methyl]phosphonic acid, dimethyl ester A. 2,2-Dimethylpropanoic acid, (E,E)-15 3,7,ll-trimethyl-10-[[(4-methylphenyl)- sulfonylloxyl-2,6-dodecadienyl ester A solution of 1.56 g (5.55 mmol) of Example 18 Part D aldehyde in 15 ml of tetrahydrofuran at -78°C was added 2.9 ml (5.80 mmol) of 2 M 2-propyl-20 magnesium chloride, in diethyl ether over 10 minutes. After 30 minutes, the reaction was allowed to warm to 0°C for 1 hour. A solution of 1.11 mg (5.82 mmol) of p-toluenesulphonyl chloride in 6 ml of tetrahydrofuran was added rapidly via 25 cannula, and the reaction was allowed to stir for 4.5 hours at 0°C. The mixture was diluted with diethyl ether, washed successively with 1 M HCl, water, saturated NaHCO^ and brine, dried (MgSO^) and evaporated to provide 2.53 g of crude 30 tosylate. Flash chromatography on 200 g of silica gel, eluted with 4:96 ethyl acetate:hexane gave 1.98 g (75%) of pure title tosylate as a thick, colorless oil. ? k -A ^ F, X TLC Silica gel (10:90 ethyl acetate:hexane) R^=0.24. IR (CC14) 2969, 2931, 1726, 1479, 1457, 1367, 1345, 1281, 1176, 1152, 905 cm1. 1H NMR (CDC13 ) 67.79 (d, 2H, J=8 Hz) 7.32 (d, 2H, J=8 Hz) .32 (td, 1H, J=7,1 Hz) .02 (td, 1H, J=7,1 Hz) 4.57 (d, 2H, J=7 Hz) 4.50 (g, 1H, J=5 Hz) 2.44 (s, 3H) 1.80-2.20 (m, 7H) 1.69 (s, 3H) 1.68 (m, 2H) 1.52 (s, 3H) 1.19 (s, 9H) 0.84, 0.85 (two d, 6H total, J=7 Hz) ppm.

Mass Spec (CI-CHA/N20, + ions) m/e 496 (M+NH^), 377, 205.

B. (E,E)-3,7,ll-Trimethyl-2,6-dodecadien- l-ol To a stirred solution of 1.98 g (4.16 mmol) Part A tosylate in 25 ml of tetrahydrofuran at 0°C 25 under argon was added 25 ml (25 mmol) of 1 M LiB(C2H5)3H in tetrahydrofuran over 30 minutes.

The solution was maintained at 0°C for 24 hours, then cooled to -20°C and the excess hydride was slowly quenched with water. The mixture was 30 treated with 12.5 ml of 10% NaOH, followed by the cautious, dropwise addition of 11 ml of 30% hydrogen peroxide. After 30 minutes, the reaction was allowed to warm to room temperature for 1 hour 9 L L s ? £«. s ... ij •,.? ^ j and then diluted with 300 ml of 1:1 diethyl ether:hexane. The organic layer was washed with water (three-100 ml portions) and brine, dried (MgSC>4), and evaporated to provide 951 mg of crude 5 alcohol. This material contained about 8% of farnesol, which was separated by chromatography on 80 g of 20% AgN03 on silica gel, packed in 10:90 and eluted with 20:80 ethyl acetate:hexane. The appropriate fractions were combined and further 10 purified by flash chromatography on 70 g of silica gel, eluted with 13:87 ethyl acetate:hexane to provide 732.5 mg (78%) of pure title alcohol as a colorless oil.

TLC Silica gel (20:80 ethyl acetate:hexane) R^=0.29. 15 IR (CCl^) 3622, 2955, 2930, 2870, 1665, 1467, 1383, 1366, 994 cm"1. 1H NMR (CDC13) 55.41 (td, 1H, J=7,1 Hz) .09 (td, 1H, J=7,1 Hz) 4.14 (d, 2H, J=7 Hz) 2.07 (m, 4H) 1.94 (t, 2H, J=7 Hz) 1.68 (s, 3H) 1.58 (s, 3H) 1.56 (nonet, 1H, J=7 Hz) 1.37 (m, 2H) 1.12 (m, 2H) 0.87 (d, 6H, J=7 Hz) ppm.

Mass Spec (CI-NH3, + ions) m/e 242 (M+NH^), 224 30 (M+H), 207 (M+H-H20), 193. p 6 A 7 •-? 7 C. (E,E)-[[(3,7,ll-Tnmethyl-2,5-dodeca-dienyl)oxy]methyl]phosphonic acid, bis(1-methylethyl) ester To a stirred solution of 490.7 mg (2.19 5 mmol) of Part B alcohol in 6 ml of THF at -78°C under argon was added 1.4 ml (2.24 mmol) of 1.6 M n-butyllithium in hexane over 15 minutes. After 40 minutes at -78°C, a solution of 735 mg (2.24 mmol) of Example 1 Part 3 triflate in 5 ml of THF 10 was added via cannula. The reaction was maintained at -78°C for 1 hour, and then allowed to warm to 0°C for 3 hours. After quenching with saturated NH^Cl, the mixture was extracted with diethyl ether and the organic layer was washed 15 with water and brine, dried (MgSO^) and evaporated to provide 0.88 g of a crude yellow liquid. Flash chromatography on 70 g of silica gel packed in 13:87 and eluted with 27:73 ethyl acetate:hexane provided 786.7 mg (89%) of pure title ether as a 20 colorless oil.

TLC Silica gel (50:50 ethyl acetate:hexane) R^=0.25. IR (CC14) 2978, 2955, 2931, 1466, 1385, 1256, 1107, 990 cm"1.

XH NMR (CDC13) 65.33 (t, 1H, J=6.5 Hz) .09 (t, 1H, J=7 Hz) 4.77 (m, 2H) 4.12 (d, 2H, J=6.5 Hz) 3.68 (d, 2H, J=8.8 Hz) 2.07 (m, 4H) 1.95 (t, 2H, J=7 Hz) 1.68 (s, 3H) 1.58 (s, 3H) 2 4 4 5:; 3 1.53 (m, 1H) 1.33, 1.35 (two d, 12H total, J=6.5 Hz) 1.20-1.40 (m, 2H) 1.13 (ra, 2H) 0.87 (d, J=7 Hz) ppm.

Mass Spec (CI-NH3, + ions) m/e 420 (M+NH^), 403 (M+H), 223, 197.

D. (E,E)-[[(3,7, ll-Trimethyl-2,6-dodecadienyl)oxy]methyl]phosphonic 10 acid, mono(1-methylethyl) ester A solution of 565.6 mg (1.41 mmol) of Part C ether in 8 ml of 2-propanol was treated with 8 ml of 1 M KOH and was heated to reflux under nitrogen for 48 hours. The 2-propanol was evaporated and 15 the aqueous solution was stirred with dichloromethane and acidified with 10% HCl. The organic layer was washed with water and brine, dried (MgSO^) and evaporated to provide 479.9 mg (95%) of title compound as a colorless oil.

TLC Silica gel (8:1:1 n-C3H?0H:con NH3:H20) Rf=0.56.

XH NMR (CDC13) 65. 33 (t, 1H, 3=1 Hz) . 09 (t, 1H, J=6.5 Hz) 4. 77 (m, 1H) 4. 13 (d, 2H, 3=1 Hz) 3. 72 (d, 2H, J=8.8 Hz) 2. 07 (m, 4H) 1. 95 (t, 2H, 3=1.5 Hz) 1. 67 (S/ 3H) 1. 58 (s, 3H) 1. 53 (m, 1H) 1. 34 (d, 6H, J =6 Hz) 1. - 1.4C (m, 2H) 1.13 (m, 2K) 0.86 (d, 6H, J=6.5 Hz) ppm.

E. (E,E)-[[(1-Methylethoxy)[[(3,7,11-5 trimethyl-2,6-dodecadienyl)oxy]methyl]- phosphinyl]methyl]phosphonic acid, dimethyl ester To a stirred solution of 479.9 mg (1.33 mmol) of Part D compound in 5 ml of CH2Cl2 under 10 argon was added 0.50 ml (2.66 mmol) of diethyl(trimethylsilyl)amine and the reaction was allowed to stir for 1.5 hours at room temperature. The solvent was evaporated, the remainder was dissolved in benzene, the solution 15 was concentrated and the residual oil was pumped at high vacuum. To the residue in 5 ml of CH2C12 containing a drop of DMF at 0°C under nitrogen was added 0.24 ml (2.75 mmol) of oxalyl chloride over 15 minutes. After 45 minutes at 0°C, the mixture 20 was allowed to warm to room temperature for 30 minutes. The solvent was evaporated, and the remainder was dissolved in benzene, the solution was evaporated and the residual orange oil was pumped at high vacuum.

In a separate flask, to 0.32 ml (2.93 mmol) of dimethyl methylphosphonate in 7 ml of THF at -78°C under argon was added 1.8 ml (2.86 mmol) of 1.6 M n-butyllithium in hexane over 10 minutes. After 30 minutes, a solution of the above acid 30 chloride in 5 ml of THF was added over 15 minutes. The reaction was allowed to stir for 1.5 hours at -78°C and was quenched with saturated NH^Cl. The mixture was diluted with CH2C12 and 2 L /• 1 & T r -J ?. 1 -J water, and the aqueous layer was acidified with 10% HCl. The organic layer was separated, the aqueous layer was re-extracted with CH2C12, and the combined organic layers were washed with 5 brine, dried (MgSO^), and evaporated to provide 605 mg of a colorless oil. The crude product was purified by flash chromatography on 70 g of silica gel eluted with 2:98 CH3OH:CH2Cl2 to provide 513 mg (83%) of pure title ester as a colorless oil. 10 TLC Silica gel (5:95 CH3OH:CH2Cl2)Rf=0.27.

IR(CC14) 2954, 2930, 2369, 1465, 1385, 1256, 1229, 1063, 1036, 992, 841 cm'1.

LH NMR (CDC13) 65.32 (td, 1H, J=7,1 Hz) 15 5.08 (td, 1H, J=6.5,1 Hz) 4.80 (m, 1H) 4.12 (d, 2H, J=7 Hz) 3.79, 3.83 (two d, 6H total, J=6 Hz) 3.70-3.90 (m, 2H) 2.48 (m, 2H) 2.08 (m, 4H) 1.94 (t, 2H, J=7.5 HZ) 1.67 (s, 3H) 1.58 (s, 3H) 1.53 (m, 1H) 1.37 (d, 3H, J=6.5 Hz) 1.34 (d, 3H, J=7 Hz) 1.20-1.40 (rn, 2H) 1.12 (m, 2H) 0.86 (d, J=7 Hz) ppm.

Mass Spec (CI-NH3, + ions) m/e 484 (M+NH^), 467 (M+H), 261. 9 /. /• " - J L "t -v -J v - J Example 21 (E,E)-[[Hydroxy[[(3,7,ll-Trimethyl-2,6-dodecadienyl)-oxy]methy1]phosphinyl]methyl]phosphonic acid, trisodium salt A solution of Example 20 triester (503.3 mg, 1.08 mmol) in 7 ml of CH2C12 at room temperature under argon was treated with 0.43 ml (3.25 mmol) of 2,4,6-collidine followed by 0.86 ml (6.48 mmol) of bromotrimethylsilane. After 24 10 hours, the solution was concentrated, the residue was dissolved in benzene, the solvent was evaporated and the remainder was dried under vacuum. After dissolution in 6 ml of 1 M NaOH, the aqueous mixture was lyophilized. The crude 15 product was purified by MPLC on a 20 cm tall, 2.5 cm diameter column of CHP20P gel, eluted with water (fractions 1-15), followed by a gradient created by the gradual addition on 500 ml of acetonitrile to 500 ml of water, collecting approximately 8 ml 20 fractions. Fractions 37-47 were combined, the acetonitrile was evaporated, the aqueous solution was lyophilized and the resulting powder dried under vacuum to provide 487.3 mg (93%) of title salt as a white, amorphous solid.

TLC Silica gel (5:4:1 n-C3H7OK:con NH3:H20) Rf=0.42. IR (KBr) 3500 (br), 2953, 2927, 2869, 1635, 1456, 1382, 1191, 1135, 1105, 1076, 1050, 972 cm1. 1H NMR (D20) 55.36 (t, 1H, J=7 Hz) 5.15 (t, 1H, J=7 Hz) 4.09 (d, 2H, J=7 Hz) 3.62 (d, 2H, J=6.3 Hz) 2.08 (m, 4H) 24 4 363 1 .92 (m, 4H) 1 .65 (s, 3H) 1 .55 (s, 3H) 1 .47 (nonet, 2H, J=7 Hz) 1 .34 (quintet, 2H, J= =7 Hz) 1 .06 (q, 2H, J=7 Hz) 0 .79 (d, 3H, J=7 Hz) ppm. 31P NMR (D20) 532.4 (d, J=IO.3 Hz) 12.8 (d, J=10.3 Hz) ppm.

Mass Spec (FAB, + ions) m/e 463 (M+H), 441 (M+2H-Na).

Anal Calcd for C17H21P206Na3 x 0.90 mol (3.39%) H20: C, 42.67; H, 6.91; P, 12.94 15 Found: C, 42.98; H, 7.31; P, 13.26 Example 22A (E,E)-[[Methoxy[[(3,7,ll-trimethyl-2,6,10-dodeca-trieny1)oxy]methyl]pho sphinyl]di fluoromethy1]- phosphonic acid, diethyl ester A solution of the Example 3, Part B phosphonate monoester (585 mg, 1.77 mmol) in 16 mL of dry CH2C12 under argon atmosphere and at room temperature was treated with N,N-diethyl(trimethylsilyl)amine 25 (0.671 mL, 3.54 mmol). The resulting pale yellow solution was stirred at room temperature for 2 hours. The CH2C12 was removed from the reaction mixture and the resulting residue was evaporated one time with benzene and then placed under high 30 vacuum for 40 minutes. The resulting residue was stirred under argon in 16 mL of dry CH2Cl2. Two drops of DMF were added, and this solution was cooled to 0°C and treated dropwise with oxalyl 2 4 4- -? *y S % chloride (0.278 mL, 3.19 mmol). The reaction mixture was stirred at 0°C for 20 minutes and then warmed to room temperature. After 2.5 hours at room temperature, the C^C^ was removed on the rotavap, 5 and the dark residue was azeotroped with benzene. The residue was pumped under high vacuum to give the desired phosphonochloridate.

A solution of lithium diisopropvlamide (LDA) was prepared by the dropwise addition of n-butyl-10 lithium (0.920 mL of a 2.5 M solution in hexane, 2.29 mmol) to a 3.8 mL THF solution of diisopropylamine (0.353 mL, 2.52 mmol) which had been cooled to -78°C under argon atmosphere. After the addition of the n-butyllithium was complete, the reaction 15 was warmed to 0°C, stirred for 15 minutes, and then recooled to -78°C. A solution of diethyl difluoro-methylphosphonate (0.450 g. 2.39 mmol) in 2.7 mL of THF was then added to the LDA solution at -78°C. After stirring for 1 hour at -78°C, the reaction 20 mixture was treated quickly with a solution of the phosphonochloridate prepared above. The amber reaction mixture was stirred for 2.5 hours at -78°C, and then quenched with NH^Cl solution. After warming to room temperature, the reaction mixture 25 was diluted with 12 mL of water and 15 mL of ethyl acetate. The aqueous layer was extracted several times with ethyl acetate. The organic extracts were combined, washed with brine and dried over MgSO^. The desired title product (211 mg) was 30 purified by flash chromatography on silica gel eluting with 75% ethyl acetate/hexane.

TLC Silica gel (75% ethyl acetate/hexane) R^=0.42 O / M C 7 L n 4 0 u x) XH NMR (CDC13) 6 5.28 (m, 1H), 5.05 (m, 2H), 4.3 (m, 4H), 4.13 (d, 2H, J=7 Hz), 4.00 (d, 2H, J=5.0 Hz), 3.95 (d, 3H, J=12 Hz), 2.15 to 1.80 (m, 8), 5 1.65 (s, 6), 1.55 (s, 6H), 1.35 (t, 6H, J=7.0 Hz) ppm. 13C NMR (CDC13) 5 142.08, 135.34, 131.18, 124.23, 123.62, 119.27, 69.75 (d, J=9.46 Hz), 65.42 (t, 10 J=9.46 Hz), 63.43 (d, J=115.44), 54.01 (d, J=7.57), 39.61, 26.67, 26.25, 25.58, 17.60, 16.46, 16.35, 16.26, 15.93 ppm.

Mass Spec (CI, + ions) m/e 297 (M+-C15H25), 205 15 (M+-C7H1506F2P2) Example 22B (E,E)-[ [Hydroxy[[(3,7, ll-trimethyl-2,6,10-dodeca-trienyl)oxy]methyl]phosphinyl]difluoromethyl]-20 phosphonic acid monoethyl ester, disodium salt The Example 22A triester (6.5 mg, 0.013 mmol) was stirred under argon atmosphere in dry DMSO in the presence of dry NaCN (2.5 mg, 0.052 mmol). The reaction mixture was then heated in a 145°C oil 25 bath for 20 hours. The reaction mixture was cooled to room temperature, and the solvent was removed in vacuo to provide title compound as a tan solid.

TLC Silica gel (7:2:1 n-C^OH: con. NH4:H20) Rf=0.29 Mass Spec (FAB, + ions) m/e 525 (M+Na), 479 (M+2H-Na) n / / 1 r- T L 1 H- 0 0 3 Example 22C (E,E)-[[Hydroxy [[(3,7,ll-trimethyl-2, 6,10-dodeca-trienyl)oxy]methyl]phosphinyl]di fluoromethy1]- phosphonic acid, trisodium salt A solution of Example 22A triester in dry CHjC^ and containing 2,4,6-collidine (3eq) is stirred at 0°C under argon atmosphere. To this solution is added trimethyl silyl iodide (4eq), and the reaction is stirred at 0°C. The solvent 10 is removed from the reaction mixture and the resulting solid is treated with IN NaOH solution. The solvents are removed in vacuo. Purification on CHP20P chromatography gives the title triacid as its trisodium salt.

Example 23 (E,E)-[[[[(7,ll-Dimethyl-2,6,10-dodecatrienyl)oxy]-methyl](1-methylethoxy)phosphinyl]methyljphosphonic acid, dimethyl ester A. (E,E )-7,ll-Dimethyl-2,6,10-doaecatrien-l-ol A solution of 798 mg (3.88 mmol) of Example 16 Part B propargylic alcohol in 20 mL of tetrahydrofuran at room temperature under argon was treated dropwise with a solution of 3.45 mL (11.6 mmol, 3 25 equiv) of 3.4 M sodium bis(2-methoxyethoxy aluminum) hydride (Red-Al trademark) in 5 mL of tetrahydrofuran. The mixture was refluxed for two hours, then cooled to 0°C and quenched with 1 M ^SO^. The mixture was filtered through Celite, washing copiously with 30 water and diethyl ether. The filtrate was diluted with diethyl ether, separated, and washed with H20 and brine, dried over MgSO^ and evaporated. Purification by flash chromatography on 70 g of ? 4 4 18 "•«' / t t / I j silica gel, eluted with 1:9 ethyl acetate: hexane provided 730 mg (90%) of title allylic alcohol as a clear, colorless oil.

TLC Silica gel (2:8 ethyl acetate: hexane) 0.36 IR (CC14) 3619, 2967, 2917, 2855, 1448, 1441, 1380, 1377, 1088, 996, 970, 803, 795 cnf1 XH-NMR (CDC13, 270 MHz) 5 5.66 (m, 2H), 5.10 (m, 2H), 4.06 (d, 2H, J=4.7 Hz), 1.9-2.2 (m, 9H), 1.68 (s, 3H), 1.60 (s, 6H) ppm.

Mass Spec (CI-CH4/N20, - ions) m/e 415 (2M-H), 207 15 (M-H), 189 (M-H-H20).

B. (E,E)-[ [7,ll-Dimethyl-2,6,10-dodeca-trienyl)oxy]methyl]phosphonic acid, bis(l- methvlethyl) ester A solution of 721 mg (3.46 mmol) of Part A allylic alcohol in 12 mL of tetrahydrofuran at -78°C under argon was treated with 2.25 mL (3.63 mmol, 1.05 equiv) of a 1.6 M solution of n-butyllithium in hexanes over ten minutes and 25 allowed to stir for 15 minutes at -78°C and 15 minutes at 0°C. After cooling to -78°C, a solution of 1.193 g (3.63 mmol, 1.05 equiv) of Example 1, Part B triflate in 5 mL of tetrahydrofuran was added over three minutes. The mixture 30 was stirred for 1.5 hours at -78°C and 3 hours at 0°C, then quenched with saturated NH4C1 and diluted with 150 mL of diethyl ether. The organic phase was washed with two 30 mL portions of water 2 4 4 3 8 and 30 mL of brine, dried over MgS04 and evaporated to give 1.215 g of crude product. Purification by flash chromatography on 70 g of silica gel, eluted with 3:7 ethyl acetate: 5 hexane yielded 1.189 g (89%) of title phosphonate.

TLC Silica gel (3:7 ethyl acetate: hexane) 0.17 IR (CC14) 2979, 2932, 2916, 1385, 1375, 1257, 10 1241, 1107, 1008, 990 cm-1 1H-NMR (CDC13, 270 MHz) 6 5.73 (br dt, 1H, J=15.2, 6.1 Hz), 5.52 (dt, 1H, J=15.2, 6.5 Hz), 5.10 (m, 2H), 4.77 (m, 2H), 4.04 (d, 2H, J=6.5 Hz), 3.67 (d, 15 2H, J=8.8 Hz), 1.9-2.1 (m, 8H), 1.68 (s, 3H), 1.60 (s, 6H), 1.34 (dd, 12H, J=5.9, 1.8 Hz), ppm.

Mass Spec (CI-NELj, + ions) m/e 404 (M+NH4), 387 (M+H), 197.

C. (E,E)-[ [(7,ll-Dimethyl-2, 6,10-dodeca-trienyl)oxy]methyl]phosphonic acid, mono(l- methylethyl) ester A mixture of 1.094 g (2.83 mmol) of Part B 25 phosphonate, 28 mL of 2-propanol, and 28 mL (28 mmol, 10 equiv) of 1 M KOH was stirred at 85-90°C under argon for three days and at room temperature for three days. The solution was neutralized to pH 6 with 10% HCl and the 2-propanol was evaporated. 30 The aqueous residue was diluted with CH2C12, acidified to pH 1 and separated. Three additional OE^C^ extractions were combined with the first, 9 A A X £ X i -7 v.; u <j washed with brine, dried over MgSO^ and evaporated to obtain 968.4 mg (99%) of title phosphonic acid.

TLC Silica gel (8:1:1 n-C^OH: con. NH3:H20) Rf 0.55 IR (CC14) 2978, 2926, 2853, 1450, 1385, 1375, 1220, 1200, 1179, 1107, 1012, 780, 752 cm"1.

XH-NMR (CDC13) 6 10.75 (br, 1H), 5.73 (br dt, 1H, 10 J=15.3, 6.2 Hz), 5.52 (dt, 1H, J=15.3, 6.5 Hz), .10 (m, 2H), 4.75 (m, 1H), 4.05 (d, 2H, J=6.5 Hz), 3.71 (d, 2H, J=8.8 Hz), 1.9-2.1 (m, 8H), 1.68 (s, 3H), 1.59 (s, 6H), 1.35 (d, 6H, J=6.5 Hz) ppm.

Mass Spec (FAB, -ions) m/e 343 (M-H), 301 D. (E,E)-[[[[(7,ll-Dimethyl-2,6,10-dodeca-trienvl)oxy]methyl](1-methylethyloxy)phosphinyl] methyl] phosphonic acid, dimethyl ester A solution of 965.3 mg (2.80 mmol) of Part C phosphonic acid in 15 mL of CH2C12 at room temperature under argon was treated with 1.05 mL (5.6 mmol, 2 equiv) of N,N-diethyl(trimethylsilyl)-25 amine and stirred for 1.5 hours. The solvent was evaporated and residue was twice evaporated from benzene, then dried at high vacuum for 1/2 hour.

The residue was dissolved in 15 mL of CH„C1~ and z. 2 one drop of dimethylformamide and treated at 0°C 30 under nitrogen with 440 pL (5.04 mmol, 1.8 equiv) of oxalyl chloride. After 2.5 hours at room temperature the solvent was evaporated, and the 0 * J A t 3 residue was twice evaporated from benzene, then dried at high vacuum for 1/2 hour.

The anion solution was prepared by treating a solution of 670 pL (6.16 mmol, 2.2 equiv) of 5 dimethyl methylphosphonate in 15 mL of tetrahydrofuran at -78°C under argon with 3.75 mL (6.02 mmol, 2.15 equiv) of 1.6 M n-butyllithium in hexanes and stirring for 1/2 hour. The phosphonic acid chloride prepared above in 3 mL of tetrahydro- furan was added over ten minutes, and the mixture was stirred for 1.5 hours at -78°C. The reaction was quenched by adding a solution of 320 mL (5.6 mmol, 2 equiv) of glacial acetic acid in 1 mL of tetrahydrofuran, warming to 0°C, and adding saturated NH.Cl. After dilution with 30 mL of 4 CHjClj and 2 mL of H20, the layers were separated and the aqueous phase was re-extracted with three 30 mL portions of C^C^. The combined organic layers were washed with brine, dried over MgSO^ and evaporated to give 1.253 g of an orange oil. Purification by flash chromatography on 70 g of silica gel, eluted with 2:98 CH30H: CH2C12 provided 567.4 mg (45%) of pure title triester.

TLC Silica gel (5:95 CH3OH: CI^C^) Rf 0.39 1H-NMR (CDC13, 270 MHz) 5 5.74 (br dt, 1H, J=15.9, 6.0 Hz), 5.52 (dt, 1H, J=15.9, 6.0 Hz), 5.10 (m, 2H), 4.79 (m, 1H), 4.04 (ABX, 2H, J^ll.7, JAX=JBX=6,0 HZ)' 3,81 (m' 2H)' 3,82 (d' 3H' J=ll.l Hz), 3.80 (d, 3H, J=12.3 Hz), 2.50 (m, 2H), 1.9-2.1 (m, 8H), 1.68 (s, 3H), 1.60 (s, 6H), 1.37 (d, 3H, J=7.0 Hz), 1.34 (d, 3H, J=7.0 Hz) ppm. 0 /, /, 7 £ 7 Mass Spec (CI-CH4/N20 + ions) m/e 451 (M+H), 261, 219, 69.

Example 24 (E,E)-[[[[(7,ll-Dimethyl-2,6,10-dodecatrienyl)oxy]-methyl]hydroxyphosphinyl]methyl]phosphonic acid, trisodium salt A solution of 553.7 mg (1.24 mmol) of Example 23 triester in 7 mL of CH2C12 at room temperature 10 under nitrogen was treated with 495 pL (3.72 mmol, 3 equiv) of 2,4,6-collidine and 990 pL (7.44 mmol, 6 equiv) of bromctrimethylsilane and stirred for 18 hours. The solvent was evaporated and the residue was dissolved in 14.9 mL (14.9 mmol, 12 15 equiv) of 1 M NaOH and lyophilized overnight. Purification was by chromatography on a 2.5 cm diameter x 18 cm height column of CHP20P resin packed in water and eluted with 100 mL of H20 followed by a gradient created by the gradual 20 addition of 300 mL of CHgCN into 300 mL of H20.

Two fractions of product were collected, identical by 270 MHz ^H-NMR, one of which was contaminated with base. A second chromatography of the combined material was carried out on a 2.5 cm diameter x 25 18 cm height column of CHP20P resin loaded in H20 and eluted with 100 mL of H20 followed by a gradient created by the gradual addition of 350 mL of CH3CN into 350 mL of H20. Approximately 10 mL fractions were collected every 1.8 minutes. 30 Fractions 30-38 were combined, evaporated, lyophilized, and pump-dried overnight to obtain 365.9 mg (66%) of title salt as a white lyophilate. 9 /, A ^ ft fca "V i TLC Silica gel (5:4:1 n-C,H?OH: con. NH3: H20) Rf=0.33 IR (KBr) 3420 (br), 2969, 2924, 1648, 1639, 1164, 1106, 1073, 1051, 971 cm"1 XH-NMR (D20, 400 MHz) 5 5.79 (br dt, 1H, Jd=15.4 Hz), 5.59 (dt, 1H, J=15.4, 6.6 Hz), 5.18 (br, 1H), 5.13 (t, 1H, J=6.8 Hz), 4.00 (d, 2H, J=6.6 Hz), 3.63 (d, 2H, J=6.2 Hz), 2.05 (m, 6H), 1.97 (m, 2H), 10 1.92 (t, 2H, J=18.1 Hz), 1.63 (s, 3H), 1.56 (s, 6H) ppm 13P-NMR (D20, 36.2 MHz) 5 32.£ (d, J=8.06 Hz), 12.3 (d, J=8.06 Hz) ppm Mass Spec (FAB, + ions) m/e 469 (M+Na), 447 (M+H), 425 (M+2H-Na), 255, 226, 140, 125 Anal, calc'd for C^gH270gP23 Na*0.72 H20: 20 C, 41.84, H, 6.24; P, 13.49 Found: C, 41.84; H, 6.48; P, 13.22 Example 25 (E,E)-[[[[(3-Chloro-7,ll-dimethyl-2,6,10-dodeca-25 trienyl)oxy]methyl]ethoxyphospninyl]methyl]- phosphonic acid, dimethyl ester A. (E,E)-3-Chloro-7,ll-dimethyl-2,6,10- dodecatrien-l-ol A solution of 3.191 g (15.5 mmol) of 30 Example 16, Part B propargylic alcohol in 75 mL of tetrahydrofuran at room temperature under argon was treated dropwise over 15 minutes with 7.3 mL (24.8 mmol, 1.6 equiv) of a 3.4 M solution of 24 4 36 3 sodium bis(2-methoxyethoxy)aluminum hydride (Red-Al) in toluene and stirred for six hours. After cooling to -7S°C a solution of 4.55 g (34.1 mmol, 2.2 equiv) of N-chlorosuccinimide in 10 mL of 5 CI^C^ was added dropwise over ten minutes. The resulting mixture was stirred for one hour at -78°C and one hour at -20°C, then quenched with 8 mL of saturated aqueous Na2S203 and 8 mL of saturated aqueous sodium potassium tartrate. After 10 partitioning between 300 mL of diethyl ether and 40 mL of H20 the layers were separated. The organic phase was washed with 30 mL of saturated Na2S203, 30 mL of 1 M K2C03, 30 mL of H20 and 30 mL of brine, dried over MgSOd and evaporated to yield 15 4.522 g of an orange oil. Purification by flash chromatography on 400 g of Merck 9385 silica eluted with 1:200:200 (CH3CH2)20: CI^Cl^ hexane provided 1.620 g (43%) of title alcohol as a colorless oil.

TLC Silica gel (2.5: 47.5: 50 (C2H5)20: CH2Cl2: hexane) R^ 0.24 IR (CC14) 3618, 3400 (br), 2968, 2926, 2916, 2856, 1684, 1446, 1380, 1377, 1158, 1106, 1084, 1012 cm"1 1H-NMR (CDC13, 270 MHz) 6 5.71 (t, 1H, J=6.8 Hz), 5.09 (m, 2H), 4.28 (d, 2H, J=6.8 Hz), 2.2-2.4 (m, 4H), 1.9-2.1 (m, 4H), 1.73 (br, 1H), 1.68 (s, 3H), 1.61 (s, 3H), 1.60 (s, 3H) ppm Mass Spec (CI-NH3, + ions) m/e 260 (M + NH^), 242 (M), 224 (M-H20) ? /i [ 1 a t 'J B. (E,E)-[[(3-Chloro-7,ll-dimethyl-2,6,10-dodecatrienvl)oxy]methyljphosphonic acid, diethyl ester A solution of 1.523 g (6.26 mmol, 1.05 5 equiv) of Part A alcohol in 25 mL of tetrahydrofuran at -78°C under argon was treated with 3.9 mL (6.26 mmol, 1.05 equiv) of 1.6 M n-butyllithium in hexanes and stirred for 0.5 hours. A solution of 1.790 g (5.96 mmol) of Example 5, Part B triflate 10 in 10 mL of tetrahydrofuran was added over ten minutes. The mixture was allowed to warm to 0°C over 75 minutes and was stirred at 0°C for two hours. The reaction was diluted with 125 mL of diethyl ether and quenched with 5 mL of NH^Cl.

After separation, the organic phase was washed with 25 mL of EjO ana 25 mL of brine, dried over MgS04 and evaporated to obtain 2.145 g of crude title product. Purification required two chromatographies. The first was run on a column 20 of 200 g of silica gel eluted with 3:7 ethyl acetate:hexane to give 1.133 g of title compound and a coeluting impurity. A second flash column of 125 g of silica gel eluted with 15:85 acetone: hexane provided 796.8 mg (34%) of pure title 25 phosphonate.

TLC Silica gel (2:8 acetone: hexane) R^=0.23 IR (CC14) 2981, 2929, 2913, 2871, 2857, 1660, 1443, 1390, 1260, 1164, 1098, 1055, 1031, 969 cm-1 1H-NMR (CDC13, 270 MHz) 6 5.63 (t, 1H, J=6.45 Hz), 5.08 (m, 2H), 4.26 (d, 2H, J=6.45 Hz), 4.18 (quint, O /, /- T £ T £ 4 -J 0 3 4H, J=7.0 HZ), 3.76 (d, 2H, J=10.0 Hz), 2.37 (t, 2H, J=6.8 Hz), 2.25 (qf 2H, J=6.3 Hz), 1.9-2.1 (m, 4H), 1.68 (s, 3H), 1.61 (s, 3H), 1.60 (s, 3H), 1.35 (t, 6H, J=7.0 Hz) ppm.

Mass Spec (CI-CH4/N20, + ions) m/e 421 (M+C2H5), 393 (M+H), 357 (M+H-HC1), 169.

C. (E,E)-[[(3-Chloro-7,ll-dimethyl-2,6,10-10 dodecatrienyl)oxy]methyl]phosphonic acid, monoethyl ester A mixture of 518.9 mg (1.32 mmol) of Part B phosphonate, 6.6 mL of ethanol, and 6.6 mL (6.6 mmol, 1.5 equiv) of 1 M KOH was stirred at room 15 temperature under nitrogen for 40 hours. The mixture was neutralized to pH 6 with 10% HCl, and the ethanol was evaporated. The aqueous residue was diluted with 20 mL of CH2C12 and H20, acidified to pH 1 and separated. The aqueous 20 phase was extracted with three 25 mL portions of CH2C12. The organic layers were combined, washed with brine, dried over MgSO^, and evaporated to yield 477.5 mg (99%) of crude title phosphonic acid.

TLC Silica gel (8:1:1 n-C^OH: con NH3: H20) Rf=0.47 ■hi-NMR (CDC13, 270 MHz) 5 9.6 (br, 1H), 5.63 (t, 1H, J=5.9 Hz), 5.08 (t, 1H, J=5.9 Hz), 30 5.07 (t, 1H, J=5.9 Hz), 4.25 (d, 2H, J=5.9 Hz), 4.18 (quint, 2H, J=7.0 Hz), 3.77 (d, 2H, J=9.4 Hz), 2.36 (t, 2H, J=7.0 Hz), 2.25 (q, 2H, J=7.0 Hz), -n / / "7 T 14 o o o 1.9-2.1 (m, 4H), 1.63 (s, 3H), 1.61 (s, 3H), 1.59 (S, 3H), 1.35 (t, 3H, J=7.0 Hz) ppm.

D. (E,E)-[[[[(3-Chloro-7,ll-dimethyl-2,6,10-5 dodecatrienyl)oxy]methyl]ethoxyphosphinyl ]- methyl]phosphonic acid, dimethyl ester A solution of 476.2 mg (1.31 mmol) of Part C phosphonic acid in 8 mL of CH^C^ at room temperature under argon was treated with 500 pL 10 (2.62 mmol, 2 equiv) of N,N-diethyl(trimethylsilyl)-amine and stirred for 1.5 hours. The solvent was evaporated, the residue was twice evaporated from benzene and the residue was dried at high vacuum for 1/2 hour. The residue, dissolved in 8 mL of 15 CI^C^ and one drop of dimethyl formamide at 0°C under nitrogen, was treated with 1.2 mL (2.35 mmol, 1.8 equiv) of a 2.0 H solution of oxalyl chloride in CH^C^. The reaction was stirred for 2.5 hours at room temperature. The solvent was 20 evaporated, the residue was twice evaporated from benzene and the residue was dried at high vacuum for 1/2 hour.

The anion solution was prepared by treating a solution of 310 pL (2.86 mmol, 2.2 equiv) of 25 dimethyl methylphosphonate in 8 mL of tetrahydrofuran at -78°C under argon with 1.75 mL (2.8 mmol, 2.15 equiv) of a 1.6 M solution of n-butyllithium in hexanes over ten minutes, and stirring for 1/2 hour. The phosphonic acid chloride prepared 30 above, in 2 mL of tetrahydrofuran was added dropwise over ten minutes. After 1.5 hours the reaction was quenched with 150 pL (2.62 mmol, 2 equiv) of glacial acetic acid in 1 mL of tetrahy- ? A L 3 f T ««« i « V' drofuran, allowed to warm to 0°C and quenched with saturated NH.Cl. The mixture was Dartitioned between 30 mL of CH2Cl2 and 10 mL of f^O. The aqueous phase was re-extracted with two 30 mL 5 portions of CH2C12. The combined organic layers were washed with brine, dried over MgSO^ and evaporated to obtain 524.9 mg of crude product as a dark orange oil. Purification required two chromatographies. The first column was loaded 10 with 50 g of silica gel and eluted with 2:98 CH^OH: CH2C12 to yield 343.5 mg of product plus an impurity. The second column was run on 40 g of silica gel, eluted with 2:98 CH^OH: CH2C12 and provided 257.9 mg (42%) of pure title diester as a 15 pale yellow oil.

TLC silica gel (5:95 CH3OH: CH2Cl2) Rf 0.36 IR (CC14) 2955, 2928, 2915, 2854, 1660, 1447, 1258, 1232, 1184, 1165, 1106, 1063, 1036, 961, 842, 816, 801, 785, 779 cm"1.

LH-NMR (CDC13) 6 5.64, (t, 1H, J=5.9 Hz), 5.08 (br t, 2H, J=5.8 Hz), 4.25 (d, 2H, J=5.9 Hz), 4.19 (m, 2H), 3.86 (m, 2H), 3.82 (d, 3H, J=11.5 Hz), 3.80 (d, 3H, J=ll.5 HZ), 2.50 (m, 2H), 2.37 (br t, 2H, J=7.5 Hz), 2.25 (br q, 2H, J=7.5 Hz), 1.9-2.1 (ra, 4H), 1.67 (s, 3H), 1.61 (s, 3H), 1.60 (s, 3H), 1.36 (t, 3H, J=7.0 Hz) ppm.

Mass Spec (CI-NH3, + ions) m/e 488 (M + NH4), 471 (M+H), 247, 264 -149-Example 26 (E,E)-[[[[(3-Chloro-7,ll-dimethyl-2,6,10-dodeca-trieny1)oxy]methyl]hydroxyphosphinyl]methyl]phos- phonic acid, trisodium salt A solution of 253.0 mg (0.54 mmol) of Example triester in 4 mL of CH2C12 was treated with 215 pL (1.62 mmol, 3 equiv) of 2,4,6-collidine and 430 pL (3.24 mmol, 6 equiv) of bromotrimethylsilar.e and stirred at room temperature under nitrogen for 10 24 hours. The solvent was evaporated, and the residue was treated with 1.65 mL (1.65 mmol, 3.0 equiv) of 1 M NaOH. The aqueous solution was then adjusted to pH 14 with 1 M NaOH and lyophilized overnight. The crude lyophilate was purified by 15 chromatography on a 2.5 cm diameter x 20 cm height column of HP-20 resin loaded in water. The column was eluted with 100 mL of H20 followed by a gradient created by the gradual addition of 400 mL of CH3CN into 400 mL of H20. Approximately 10 mL 20 fractions were collected every 1.3 minutes.

Fractions 25-32 were combined, evaporated, lyophilized and dried at high vacuum overnight to obtain 245.3 mg (94%) of title salt as a white lyophilate. A 1.0% aqueous solution of title salt has pH 9.15.

TLC Silica gel (4:4:1 n-C^OH: con NH3: H20) Rf=0.31 IR (KBr) 3427 (br), 2969, 2921, 2859, 1661, 1445, 1380, 1178, 1153, 1093, 1056, 977, 873, 796, 709 30 cm""1. 1H-NMR (D20, 400 MHz) 5 5.76 (t, 1H, J=6.2 Hz), 5.13 (br, 2H), 4.22 (d, 2H, J=6.2 Hz), 3.64 (d, 2H, J=6.2 Hz), 2.38 (t, 2H, J=7.1 Hz), 2.23 (q, 2H, J=7.1 Hz), 2.06 (q, 2K, J=6.9 Hz), 1.98 (t, 2H, J=6.9 Hz), 1.93 (t, 2H, J=17.9 Hz), 1.63 (s, 3H), 1.58 (s, 3E), 1.56 (s, 3H) ppm. 31P-NMR (D20, 36.2 MHz) 6 32.34 (d, J=8.8 Hz), 12.42 (d, J=8.8 Hz) ppm.

Mass Spec (FAB + ions) m/e 503 (K+Na), 481 (M+H), 10 459 (M+2H-Na).

Anal Calcd for c16H26Cl06P2•1.05 H20 (3.79% H20): C 38.46 H 5.67 Cl 7.10 P 12.38 Found: C 38.83 H 5.93 Cl 7.48 P 12.02 Example 27 (E,E) - [ [Ethoxy [ [(3,7, ll-trimethyl-2 , 6,10-dodeca-trienyl)thio]methyl]phosphinyl]methyl]phosphonic acid, dimethyl ester A. Ethanethioic acid, S-(hydroxymethyl) ester Thiolacetic acid, obtained from Evans Chemetics, Inc., was purified prior to use by distillation from P2°5" The procedure of Boehme, H., et al, (Ann., (1959), 623, p. 92) was followed. A mixture of 10.7 mL (0.15 mol) of thiolacetic acid and 4.5 g (0.15 mol) of paraformaldehyde was stirred at 100°C for 1.5 hours. The yellow liquid was fractionally 30 distilled to obtain 12.50 g (79%) of title L\ -j /•» n compound as a yellow liquid, bp. 68-71°C/25 mm (lit. bp 68-70°C/20 mm).

TLC Silica gel (3:7 ethyl acetate: hexane) Rf=0.23. 1H-NMR (CDC13, 270 MHZ) 6 5.06 (S, 2H), 2.40 (s, 3H) ppm.

B. Ethanethioic acid, S-(bromomethyl) ester 10 A mixture of 12.35 g (0.117 mol) of Part A compound and 3.67 mL (38.7 mmol, 0.33 equiv) of phosphorus tribromide was heated at 100°C for 1/2 hour. After cooling, 40 mL of 0°C water was added, and the layers were separated. The organic phase 15 was washed with , dried over MgS04, filtered and evaporated. Fractional distillation provided 10.79 g (55%) of title compound as a pale yellow liquid with bp 38-40°C/0.8 mm.

TLC Silica gel (hexane) R^=0.26. 1H-NMR (CDC13, 270 MHz) 6 4.74, (d, 2H, J=2.11 Hz), 2.42 (d, 2H, J=2.11 Hz) ppm.

C. [(Acetylthio)methyl]phosphonic acid, diethyl ester The procedure of Farrington, G.K., et al, (J. Med. Chem., (1985), 28, 1668) was followed.

A mixture of 10.51 g (62.1 mmol) of Part B 30 compound and 11.7 mL (68.3 mmol, 1.05 equiv) of triethylphosphite was stirred at 130°C for 2.5 hours. The product was isolated by fractional 2 4 4 3 B distillation, which yielded 10.10 g (72%) of title compound as a colorless oil with bp 82-84°C/0.005 mm (lit. b.p. 105-106°C/0.03 mm).

TLC Silica gel (7:3 Ethyl acetate: hexane) Rc=0.17. 1H-NMR (CDC13, 270 MHz) 6 4.14 (quint, 4H, 3=1.1 Hz), 3.23 (d, 2H, J=14.2 Hz), 2.40 (s, 3H), 1.33 (t, 6H, 3=1.1 Hz) ppm.

D. (E,E)-3,7,ll-Trimethyl-2,6,10- dodecatrienvl bromide A solution of 1.00 g (4.5 nunol) of (E,E)-farnesol in 10 mL of distilled diethyl ether .15 at 0°C under argon in the dark was treated dropwise with a solution of 195 |jL (2.05 mmol, 0.45 eq. ) of PBr^ in 2 mL of ether. The resultant mixture was stirred at 0°C for one hour, then quenched with water and separated. The organic phase was washed 20 with 5 mL of ^0, 5 mL of saturated NaHCO^, and 5 mL of brine, dried over Na2S04 and evaporated to give 1.26 (98%) of crude bromide as a clear oil. TLC Silica gel (2:8 ethyl acetate:hexane) R^=0.69 (decomposes). 1H NMR (CDC13) 5 5.52 (t, 1H, J=8.5 Hz), 5.08 (m, 2H), 4.01 (d, 2H), 1.9-2.2 (m, 8H), 1.73 (s, 3H), 1.68 (s, 3H), 1.60 (s, 6H) ppm.

E. (E,E)- [ [ (3, 7,11-Trimethyl-2,6,10-dodeca-trienyl)thio]methyl]phosphonic acid, diethyl ester To a solution of 3.661 g (16.2 mmol, 1.2 equiv) of Part C thioacetate in 3 5 mL of freshly-distilled ethanol was added 10 mL (15 mmol, 1.1 equiv) of a freshly prepared 1.5 M solution of 5 sodium ethoxide. After two hours, a solution of 3.85 g (13.5 mmol) of Part D farnesyl bromide in 30 mL of benzene was added over 1/2 hour. The reaction was allowed to stir for 1.5 hours, then quenched with saturated NH^Cl and evaporated. The 10 residue was dissolved in water and 200 mL of diethyl ether and the layers were separated. The organic phase was washed with brine, dried over MgSO^ and evaporated to give 5.107 g of a crude oil. Purification by flash chromatography on 500 g 15 silica gel, eluted with 3:7 ethyl acetate:hexane provided 3.813 g (73%) of title compound as a clear, colorless oil.

TLC Silica gel (4:6 ethyl acetate: hexane) Rf 0.27 IR (CC14) 2980, 2926, 2915, 1444, 1375, 1218, 1046, 991, 962 cm-1 1H-NMR (CDC13, 270 MHz) 6 5.22 (t, 1H, J=7.9 Hz), 25 5.09 (m, 2H), 4.17 (quint, 4H, J=7.0 Hz), 3.34 (d, 2H, J=7.9 Hz), 2.65 (d, 2H, J=12.3 Hz), 1.9-2.2 (m, 8H), 1.69 (s, 3H), 1.63 (s, 3H), 1.60 (s, 6H), 1.35 (t, 6H, J=7.0 Hz) ppm.

Mass Spec (CI-CH4/N20, + ions) m/e 429 (M+C3H5), 417 (M+C2H5), 389 (M+H), 185. n / / 7 c *7 / h. l\ ^ r-, -i F. (E,E)-[[(3,7,ll-Trimethyl-2,6,10-dodeca-trienyl)thio]methyljphosphonic acid, monoethyl ester A mixture of 798 mg (2.06 mmol) of Part E 5 compound, 20 mL of ethanol and 20 mL (20.0 mmol, 9.7 equiv) of 1 M KOH under argon was heated at 65-70°C for 16 hours. After cooling, the mixture was neutralized to pH 6 with 10% HCl and the ethanol was evaporated. The aqueous residue was 10 diluted with 25 mL of CH2Cl2, acidified to pH 1 and separated. The aqueous phase was extracted with four 20 mL oortions of CH„C10. The combined L 2 organic layers were washed with 20 mL of brine, dried over MgSO^, and evaporated to yield 777 mg 15 (100%) of title phosphonic acid.

TLC Silica gel (8:1:1 n-C3H70H: con NH3:H20) Rf=0.60 1H-NMR (CDC13, 270 MHz) 6 10.5 (br, 1H), 5.21 (t, 20 1H, J=7.9 Hz), 5.09 (m, 2H), 4.17 (quint, 2H, J=7.0 Hz), 3.33 (d, 2H, J=7.9 Hz), 2.66 (d, 2H, J=12.9 Hz), 1.9-2.2 (m, 8H), 1.68 (s, 6H), 1.60 (s, 6H), 1.35 (t, 3H, J=7.0 Hz) ppm.

G. (E,E)-[[Ethoxy[[(3,7,ll-trimethyl-2,6,10- dodecatrienyl) thio ] methyl ] phosphinyl ] methyl ] - phosphonic acid, dimethyl ester A solution of 460 mg (1.28 mmol) of Part F phosphonic acid in 7 mL of CH2C12 at room 30 temperature under argon was treated with 490 pL (2.58 mmol, 2 equiv) of N,N-diethyl(trimethylsilyl)-amine and was allowed to stir for 1.5 hours. The solvent was evaporated, the residue was twice 9 / /, ^ •? T ft J "W j evaporated from benzene and dried at high vacuum. The residue was dissolved in 3 mL of CYL^Cl^ at 0°C under nitrogen and was treated with 1 drop of dimethyl formamide and dropwise with 200 pL (2.30 5 mmol, 1.8 equiv) of oxalyl chloride, then stirred at room temperature for 2.5 hours. The solvent was evaporated, the residue was twice evaporated from benzene and dried at high vacuum for 1/2 hour. The residue was dissolved in 2.0 mL of 10 tetrahydrofuran.

The anion solution was prepared by treating a solution of 160 pL (1.47 mmol, 2.3 equiv) of dimethyl methylphosphonate in 3 mL of tetrahydrofuran at -78°C under argon with 880 pL 15 (1.41 mmol, 2.2 equiv) of a 1.6 M solution of n-butyllithium in hexanes over five minutes. After 1/2 hour, 525.4 mg (1.41 mmol, 2.2 equiv) of CeCl^'V H20 (dried for 2 hours at 140°C at high vacuum) was added and the resulting suspension was 20 stirred for one hour. One half (1.0 mL) of the solution of phosphonic acid chloride prepared above was added over five minutes. The reaction was stirred two hours at -78°C then quenched with a solution of 75 pL (1.31 mmol, 2 equiv) of 25 glacial acetic acid in 0.5 mL of THF. After warming to 0°C the reaction was quenched with saturated NH.Cl. The mixture was diluted with ethyl acetate and H20 and separated. The aqueous phase was extracted with three 20 mL portions of 30 ethyl acetate, and the organic layers were combined, washed with water and brine and dried over MgSO^ to yield 168.2 mg of crude product mixture. Flash chromatography on 15 g of silica gel, eluted with 0 /, /. ? e- y f •>,! 7 f t ; ^ 2:98 CH3OH: CH2C12 provided 51.8 mg (17%) of pure title compound as a clear, colorless oil.

TLC Silica gel (3:7 acetone: ethyl acetate) R^=0.24 IR (CC14) 3468 (br), 2961, 2917, 2854, 1448, 1384, 1376, 1246, 1184, 1033, 960, 846, 819 cm"1 1H-NMR (CDC13, 270 MHz) 6 5.23 (td, 1H, J=8.2, 1.2 Hz), 10 5.09 (m, 2H), 4.20 (quint, 2H, J=7.0 Hz), 3.81 (d, 3H, J=12.3 Hz), 3.80 (d, 3H, J=ll.l Hz), 3.37 (ABX, 2H, ^=13.5 Hz, JAX=JB>,=8 Hz), 2.85 (ABX, 2H, Jab=12 Hz, J^Jg^ll Hz), 2.62 (dd, 2H, J=17.0, 21.1 Hz), 1.9-2.1 (m, 8H), 1.70 (s, 3H), 1.68 (s, 15 3H), 1.60 (s, 6H), 1.36 (t, 3H, J=7.0 Hz) ppm Mass Spec. (CI-CH4/N20, + ions) m/e 507 (M+C3H5), 495 (M+C2H5), 467 (M-^H), 263 Example 28 (E,E)-[[Hydroxy[[(3,7,ll-trimethyl-2,6,10-dodeca-trienyl)thio]methyl]phosphinyl]methyl]phosphonic acid, trisodium salt A solution of 56.3 mg (0.12 mmol) of Example 25 27 triester in 2 mL of CH2C12 at room temperature under nitrogen was treated with 49 pL (0.36 mmol, 3 equiv) of 2,4,6-collidine and 100 pL (0.72 mmol, 6 equiv) of bromotrimethylsilane and stirred for 24 hours. The solvent was evaporated and pumped under 30 vacuum. The residue was dissolved in 1.5 mL of H20, treated with 400 pL (0.40 mmol, 3.3 equiv) of 1 M NaOH and lyophilized. The brown lyophilate was >• 4 U \ 8 A C '.J dissolved in 3 mL of H20 and purified by chromatography on a 1.5 cm diameter x 24 cm height column of CHP20P resin loaded in water. The column was eluted with 50 mL of H20 followed by a gradient created by 5 the gradual addition of 150 mL of CH3CN into 150 mL of H20. Approximately 5 mL fractions were collected every minute. Fractions 33-38 were combined, evaporated, lyophilized and dried at high vacuum to obtain 53.4 mg (92%) of title product as a 10 white lyophilate.

TLC Silica gel (4:4:1 n-C3H7OH: con NH3:H20) Rf=0.62 IR (KBr) 3428 (br), 2965, 2920, 2854, 1658, 1652, 15 1635, 1446, 1380, 1171, 1096, 1055, 897, 796 cm"1. 1H-NMR (D20, 400 MHz) 6 5.27 (t, 1H, J=8.0 Hz), 5.13 (m, 2H), 3.28 (d, 2H, J=8.0 Hz), 2.72 (d, 2H, J=ll.0 Hz), 1.9-2.1 (m, 10H), 1.64 (s, 3H), 1.63 20 (s, 3H), 1.56 (s, 6H) ppm. 31P-NMR (D20, 36.2 MHz) 6 33.0 (d, J=6.6 Hz), 14.0 (d, J=6,6 Hz) ppm.

Mass Spec (FAB, + ions) m/e 499 (M+Na), 477 (M+H), 455 (M+2H-Na) Anal Calcd for C17H2g05P2S•3Na-0.31 mole H20: C, 42.37; H, 6.19; P, 12.85 Found: C, 42.37; H, 6.26; P, 12.71 2 4 3 6 3 -158-Example 29 (S,E)-[[ [ [ (3-Ethyl-7,ll-dimethyl-2, 6,10-dodeca-trienyl)oxy]methyl](1-methylethoxy)phosphinyl]methyl ]- phosphonic acid, dimethyl ester A. (E)-5,9-Dimethyl-2-(1-oxopropyl)-4,8- decadienoic acid, ethyl ester To a suspension of 3.92 g (97.5 mmol, 3 equiv) of 60% NaH in mineral oil (washed three times with pentane) in 200 mL of tetrahydrofuran 10 at 0°C under argon was added dropwise a solution of 15.3 mL (107 mmol, 3.3 equiv) of ethyl propionylacetate. After 0.5 hour, a solution of 7.04 g (32.4 mmol) of (E)-geranyl bromide in 20 mL of tetrahydrofuran was added dropwise over 45 15 minutes. The mixture was stirred one hour at 0°C and one hour at room temperture, quenched with NHjCl and diluted with 500 mL of diethyl ether. The organic layer was washed with water and brine, and dried over MgS04 to obtain 20.157 g of crude 20 product as a yellow oil. Purification by flash chromatography on 800 g of silica gel, eluted with 3:97 ethyl acetate:hexanes provided 7.024 g (77%) of pure product as a clear, colorless oil.

TLC Silica gel (5:95 ethyl acetate:hexane) R^.=0.31 IR (CCL4) 2980, 2929, 2915, 2856, 1743, 1717, 1475, 1458, 1446, 1411, 1377, 1368, 1347, 1331, 1300, 1264, 1231, 1193, 1152, 1108, 1080, 1034, 30 798, 790, 775, 760, 748, 737 cm"1. ■hl-NMR (CDC13, 270 MHz) 8 5.05 (m, 2H), 4.17 (q, 2H, J=7.04 Hz), 3.46 (t, 1H, J=7.62 Hz), 2.4-2.7 ■r / ! 7 ^ y (m, 4H), 1.9-2.1 (m, 4H), 1.67 (s, 3H), 1.62 (s, 3H), 1.59 (s, 3H), 1.25 (t, 3H, J=7.04 Hz), 1.06 (t, 3H, J=7.04 Hz) ppm.

Mass Spec (CI-CH4/N20, + ions) m/e 281 (M+H), 263.

B. (£)-7, ll-Dimethyl-6,10-dodecadien-3-one A mixture of 7.776 g (27.7 mmol) of Part A keto-ester in 80 mL of 15% NaOH at 60-63°C under 10 argon was stirred for 75 minutes, then allowed to cool to room temperature. The mixture was diluted with 200 mL of diethyl ether and separated. The aqueous phase was re-extracted with 200 mL of diethyl ether. The combined organic layers were 15 washed with 8 mL of water and 80 mL of brine, dried over MgSO^ and evaporated to give 5.496 g of crude product as a clear, colorless oil.

Purification by bulb-to-bulb distillation at 120°C/0.05 mm provided 4.823 g (83%) of pure title * ketone.

TLC Silica gel (5:95 ethyl acetate:hexane) R^=0.44 IR (CCl^) 2971, 2915, 2855, 1718, 1529, 1510, 1500, 1450, 1413, 1377, 1353, 1261, 1238, 1196, 1157, 1111, 1064, 974, 946, 836, 794, 773, 758, 753, 748, 736, cm 1H-NMR (CDC13, 270 MHz) 5 5.08 (m, 2H), 2.43 (t, 2H, J=7 Hz), 2.41 (q, 2H, J=7 Hz), 2.26 (q, 2H, J=7 Hz), 2.00 (m, 4H), 1.67 (s, 3H), 1.61 (s, 3H), I 1.59 (s, 3H), 1.05 (t, 3H, J=7 Hz) ppm. 24 4 3 6 Mass Spec (CI-NH-j, + ions) m/e 225 (M+NH^), 209 (M+H), 191, 165 C. (E,E)-3-Ethyl-7,ll-dimethyl-2,6,10-5 dodecatrien-l-ol (2E, 6E-isomer) D. (Z,E)-3-Ethyl-7,ll-dimethyl-2,6,10- aodecatrien-l-ol (2Z, 6E-isomer) A solution of 2.30 mL (11.5 mmol, 1.2 equiv) 10 of triethyl phosphonoacetate in 40 mL of tetrahydrofuran at 0°C under argon was treated dropwise over 20 minutes with 11.5 mL (11.5 mmol, 1.2 equiv) of 1 M sodium bis(trimethylsilyl )amide in tetrahydrofuran. After warming to 15 room temperture, a solution of 2.008 g (9.60 mmol) of Part B ketone in 5 mL of tetrahydrofuran was added over 15 minutes and the mixture was refluxed for 24 hours. After cooling, the solvent was evaporated and the gummy orange residue was 20 dissolved in 40 mL of hexane and 15 mL of water. The aqueous layer was extracted with two additional portions of 35 mL of hexane. The combined organic layers were washed with brine, dried over MgSOd, and evaporated to give 2.465 g 25 of crude title product as an isomer ratio of approximately 1.5:1 2E:2Z isomers. Purification by flash chromatography on 200 g silica gel, eluted with 2:8 toluene:hexane provided 256 mg (15%) of 3-ethyl-7,ll-dimethyl-2,6,10-dodecatrienoic 30 acid, ethyl ester as a 1:10 2E:2Z mixture, and 1.60 g (62%) as a 2:1 2E:2Z mixture.

A solution of 1.327 g (4.77 mmol) of the above ester isomers (2:1 2E:2Z) in 25 mL of dry 9 h J R -161- i ~ v toluene at 0°C was treated over 30 minutes with 9.5 mL (14.3 mmol, 3 equiv) of a 1.5 M solution of diisobutylaluminum hydride in toluene. After two hours, the reaction was quenched with 250 pL (6.2 5 mmol, 1.3 equiv) of methanol, then with 250 pL of water, 250 pL of 15% NaOH, and 750 pL of water. After stirring for 0.5 hour, Na2S04 was added and the reaction stirred an additional one hour before filtering through a pad of Celite, washing 10 copiously with diethyl ether. Evaporation provided 1.014 g of crude product. Purification was accomplished by a series of flash chromatographies run on 20% silver nitrate on silica gel eluted with 3:7 ethyl acetate:toluene, followed by flash 15 chromatography on normal silica gel, eluted with 7:93 ethyl acetate:hexane. In this manner was obtained 195 mg (17%) of pure title 2Z, 6E-isomer and 433 mg (39%) of pure title 2E, 6E-isomer. 2E, 6E-Isomer Data: TLC Silica gel (2:8 ethyl acetate:hexane) R^=0.34 IR (CC14) 3620, 2967, 2929, 2877, 2857, 1661, 25 1451, 1381, 1376, 1327, 1308, 1267, 1255, 1222, 1214, 1182, 1178, 1153, 1106, 11082, 1066, 1048, 1003, 974, 939, 838 cm-1.

XH-NMR (CDC13, 400 MHz) 5 5.38 (t, 1H, J=6.8 Hz), 30 5.11 (m, 2H), 4.16 (d, 2H, J=5.5 Hz), 2.0-2.1 (m, 8H), 1.98 (t, 2H, J=7.3 Hz), 1.68 (s, 3H), 1.60 (s, 6H), 1.12 (br s, 1H), 0.99 (t, 3H, J=7.3 Hz) ppm. o V, / 7 P "7 £ *y d 0 0 Mass Spec (CI-NH3# + ions) m/e 236 (M), 219 (M+H-H20), 149, 135, 123, 69 2Z, 6E-Isomer Data: . TLC Silica gel (2:8 ethyl acetate:hexane) R^=0.37 IR (CC14) 3621, 2966, 2929, 2879, 2858, 1662, 10 1451, 1381, 1376, 1226, 1107, 1058, 994, 940, 839 cm-1. 1H-HMR (CDC13, 400 MHz) 5 5.41 (t, 1H, J=6.96 Hz), 5.09 (m, 2H), 4.14 (d, 2H, J=6.96 Hz), 1.9-2.1 (m, 15 10H), 1.68 (s, 3H), 1.60 (s, 6H), 1.16 (br s, 1H), 1.03 (t, 3H, J=7.5 Hz) ppm.

Mass Spec (CI-NH3, + ions) m/e 236 (M+H), 219 (M+H-H20), 149, 135, 123, 69.

E. (E,E)-[[(3-Ethy1-7,ll-dimethyl-2, 6,10-dodecatrienyl)oxy]methyl]phosphonic acid, bis(1-methylethyl) ester A solution of 392 mg (1.66 mmol) of Part C 25 alcohol (2E, 6E-isomer), in 5 mL of tetrahydrofuran at -78°C under argon was treated with 1.05 mL (1.66 mmol, 1.0 equiv) of a solution of 1.6 M n-butyllithium in hexanes and stirred for 0.5 hours. A solution of 601 mg (1.82 mmol, 1.1 equiv) of Example 1, Part B triflate in 3 mL of tetrahydrofuran was added rapidly via cannula. n i i. C 4 ,3 —163 — The reaction was stirred 0.5 hour at -78°C and two hours at 0°C, then quenched with NH^Cl and diluted with 80 mL of diethyl ether and 20 mL of water. The organic phase was washed with 20 mL of 5 water and 20 mL of brine, dried over MgSO^ and evaporated to give 892 mg of crude product. Chromatography on 70 g of silica gel, eluted with 1:1 diethyl ether:hexane provided 552 mg (83%) of title phosphonate as clear, colorless oil.

TLC Silica gel (1:1 ethyl acetate:hexane) R^=0.32 IR (CC14) 2978, 2933, 2877, 1385, 1375, 1257, 1241, 1107, 1006, 990, 773, 750, 738 cm"1.

■"•H-NMR (CDC13, 270 MHz) 5 5.28 (t, 1H, J=7.04 Hz), 5.10 (m, 2H), 4.77 (m, 2H), 4.14 (d, 2H, J=7.04 Hz), 3.69 (dd, 2H, J=8.80, 1.76 Hz), 1.9-2.1 (m, 10H), 1.68 (s, 3H), 1.60 (s, 6H), 1.34 (d, 12H, 20 J=5.67 Hz), 0.98 (dd, 3H, J=7.63, 1.76 Hz) ppm Mass Spec (CI-CH4/N20, + ions) m/e 455 (M+C3H5), 443 (M+C2H5), 415 (M+H), 197 F. (E,E)-[[(3-Ethyl-7,ll-dimethyl-2,6,10- dodecatrienyl)oxy]methyl]phosphonic acid, mono(1-methylethyl) ester A mixture of 536 mg (1.28 mmol) of Part E phosphonate, 6.40 mL (6.4 mmol, 5 equiv) of 1 M 30 KOH, and 6.40 mL of isopropanol was stirred at 85-90°C under nitrogen for 24 hours. After cooling to room temperature, the isopropanol was evaporated and the residue was diluted with 50 mL 24 4 5 6 of CH2C12, acidified with 10% HCl, and separated. The aqueous phase was re-extracted with 50 mL of CH2C12. The combined organic layers were washed with brine, dried over MgSO^, and evaporated to 5 obtain 493 mg (100%) of crude title monophospnonic acid.

TLC Silica gel (8:1:1 n-C3H7OH:Con NH3:H20) Rff=0.59 1H-NMR (CDC13, 270 MHz) <5 5.29 (t, 1H, J=7.04 Hz), 5.10 (m, 2H), 4.76 (m, 1H), 4.15 (d, 2H, J=7.04 Hz), 3.72 (d, 2H, J=8.79 Hz), 1.9-2.1 (m, 10H), 1.68 (s, 3H), 1.60 (s, 6H), 1.35 (d, 6H, J=5.86 Hz), 0.98 (t, 3H, J=7.63 Hz) ppm.

G. (E,E)-[[[[(3-Ethyl-7,ll-dimethyl-2,5,10-dodecatrienyl)oxy]methyl](1-methylethoxy)-phosphinyl]methyl]phosphonic acid, dimethyl ester A solution of 481 rag (1.29 mmol) of Part F phosphonic acid in 7 mL of dry CH2C12 at room temperature under argon was treated with 490 pL (2.58 mmol, 2.0 equiv) of N,N-diethyltrimethylsilyl-amine and allowed to stir for 1.5 hours. The solvent 25 was evaporated and the residue was evaporated from benzene and dried at high vacuum for 0.5 hour.

The residue was dissolved in 7 mL of dry CH2C12 at 0°C under nitrogen and treated with one drop of dimethyl formamide and 1.30 mL (2.58 mmol, 30 2.0 equiv) of a 2.0 M solution of oxalyl chloride in CH«Cl«. The reaction was stirred for two hours at room temperature and the solvent was evaporated. 0 A L 7 £ 7 cL h J 0 J The residue was evaporated from benzene and dried at high vacuum for 0.5 hour.

The anion solution was prepared by treating dropwise over five minutes a solution of 310 pL 5 (2.84 mmol, 2.2 equiv) of dimethyl methylphosphonate in 7 mL of tetrahydrofuran at -78°C under argon with 1.73 mL (2.77 mmol, 2.15 equiv) of a 1.6 M solution of n-butyllithium in hexanes. After 0.5 hour, the phosphonic acid chloride prepared above 10 was added dropwise in a solution of 3 mL of tetrahydrofuran. The reaction was stirred two hours at -78°C, quenched with saturated NH4C1, diluted with 5 mL of CH2C12 and allowed to warm to room temperature. Additonal 50 mL of CH2Cl2, 5 mL 15 of water and 5 mL of 1 M KCl was added and the layers were separated. The aqueous phase was re-extracted with 50 mL of CH2C12 • The combined organic layers were washed with brine, dried over MgS04, and evaporated to give 662 mg of crude 20 material as an orange oil. Purification by flash chromatography on 65 g of silica gel, eluted with 2:98 CH30H:CH2C12 provided 488 mg (73%) of desired title triester.

TLC Silica gel (5:95 CH3OH:CH2C12) Rf=0.27 IR (CC14) 2968, 2930, 2876, 2854, 1451, 1375, 1257, 1230, 1105, 1089, 1063, 992, 841 cnT1 1H-NMR (CDC13, 270 MHz) 6 5.27 (t, 1H, J=7.04 Hz), .09 (m, 2H), 4.80 (m, 1H), 4.13 (d, 2H, J=7.04 Hz), 3.82 (d, 2H, J=5.35 Hz), 3.82 (d, 3H, J=11.4 Hz), 3.80 (d, 3H, J=ll.4 Hz), 2.50 (m, 2H), 1.9-2.1 (m, 24 4 3 6 3 10H), 1.58 (s, 3H), 1.60 (s, 6H), 1.36 (t, 6H, J=7.3 Hz), 0.98 (t, 3H, J=7.65 Hz) ppm.

Mass Spec (CI-NH4, + ions) m/e 496 (M+NH4), 479 5 (M+H) Example 30 (E,E)-[[[[(3-Ethyl-7,ll-dimethyl-2,6,10-dodeca-trienyl)oxy]methyl]hydroxyphosphinyl]methyl]phos- phonic acid, trisodium salt A solution of 473 mg (0.99 mmol) of Example 29, triester in 5 mL of dry CHjC^ at room temperature under argon was treated with 390 pL (2.97 mmol, 3 equiv) of 2,4,6-collidine and 785 pL 15 (5.94 mmol, 6 equiv) of bromotrimethylsilane and stirred for 28 hours. The solvent was evaporated and the residue was treated with 3.0 mL of 1 M NaOH to pH 14 and lyophilized. Purification was effected by chromatography on a 2.5 cm diameter x 20 20 cm height column of CHP20P resin packed in water and eluted with 100 mL of water followed by a gradient created by the gradual addition of 400 mL of CH^CN into 400 mL of water. Approximately 10 mL fractions were collected every 1.5 minutes. 25 Fractions 29-35 were combined, evaporated, lyophilized and dried at high vacuum overnight to obtain 140 mg (30%) of pure title compound as a white lyophilate. (Additional 108 mg (23%) of product containing a trace impurity were obtained from fractions 36-38).

TLC Silica gel (4:4:1 n-C3H70H:con NH3:H20) Rf=0.40. 24 4 3*3 IH (KBr) 3435, 2966, 2926, 2858, 1652, 1191, 1150, 1138, 1130, 1107, 1078, 1052 cm"1 1H NMR (D20, 400 MHz) 6 5.33 (t, 1H, J=7 Hz), 5.15 (in, 2H), 4.11 (d, 2H, 3=1 Hz), 3.64 (d, 2H, J=6.2 Hz), 2.07 (m, 8H), 1.93 (m, 2H), 1.82 (t, 2H, J=18 Hz), 1.63 (s, 3H), 1.56 (s, 6H), 0.92 (t, 3H, J=7.5 Hz) ppm Mass Spec (FAB, + ions) m/e 497 (M+Na), 475 (M+H) Anal Calcd for C18H32P206Na3 x 2.29 mol H20: C, 41.99; H, 6.95; P, 12.03 15 Found: C, 41.59; H, 6.54; P, 11.85 Example 31 (22,6E)-[[[[(3-Ethvl-7,10-dimethyl-2,6,10-dodeca-trienyl)oxy]methyl](1-methylethoxy)phosphinvl]- methyl]phosphonic acid, dimethyl ester A. (2Z,6E)-[[(3-Ethyl-7,ll-dimethyl-2,6,10-dodecatrienyl)oxy]methyl]phosphonic aicd, bis(1-methylethyl) ester A solution of 336 mg (1.42 mmol) of Example 25 29, Part D alcohol (approximately 95:5 2Z:2E) in 5 mL of tetrahydrofuran at -78°C under argon was treated dropwise over three minutes with 0.90 mL (1.44 mmol, 1.0 equiv) of a 1.6 M solution of n-butyllithium in hexanes and allowed to stir for 30 0.5 hour. A solution of 519 mg (1.58 mmol, 1.1 equiv) of Example 1, Part B triflate in 3 mL of tetrahydrofuran was added rapidly by cannula. The 2 4 4 3 reaction was stirred one hour at -78°C and four hours at 0°C, quenched with NH4C1 and diluted with 100 mL of diethyl ether. The organic phase was washed with two 20 mL portions of water and 20 mL 5 of brine, dried over MgSO^ and evaporated.

Purification by flash chromatography on 70 g of silica gel, eluted with 2:8 ethyl acetate:hexane failed to separate unreacted triflate. A second chromatography on 35 g silica gel, eluted with 1:9 10 ethyl acetateiCE^C^ provided 321 mg (53%, 65% based on recovered starting alcohol) of desired title product.

TLC Silica gel (1:1 ethyl acetate:hexane) R^=0.25 IR (CCla) 2979, 2933, 2877, 1452, 1429, 1386, 1375, 1258, 1246, 1220, 1145, 1107 cm"1 1H NMR (CDC13, 270 MHZ) 6 5.30 (t, 1H, J=7.0 Hz), 20 5.09 (br, 2H), 4.75 (m, 2H), 4.14 (d, 2H, J=7.0 Hz) 3.69 (d, 2H, J=8.80 Hz), 1.9-2.1 (m, 10H), 1.68 (s, 3K), 1.60 (s, 6H)1.34 (d, 12H, J=6.45 Hz), 1.02 (t, 3H, J=7.b Hz) ppm Mass Spec (CI-CH^/^O, + ions) m/e 455 (M+C^H^), 443 (M+C2H5), 415 (M+H), 197, 144 B. (2Z,6E)-[[(3-Ethyl-7, ll-dimethyl-2,6,10-dodecatrienyl)oxy]methylJphosphonic acid, mono(l-methylethvl) ester A mixture of 311 mg (0.75 mmol) of Part A phosphonate, 3.75 mL (3.75 mmol, 5 equiv) of 1 M KOH, and 3.75 mL of isopropanol was stirred at 9 /, &m f / >8 •wYJi < £ 7 ? J 85-90°c for 24 hours. The isopropanoi was evaporated, and the residue was diluted with 50 mL of diethyl ether, acidified with 10% HCl, and separated. The aqueous phase was re-extracted 5 with 50 mL of diethyl ether. The combined organic layers were washed with 20 mL of brine, dried over MgSO^, and evaporated to provide 262 mg (94%) of crude title phosphonic acid.

TLC Silica gel (8:1:1 n-C3H7OH:con NH3--H20) Rf=0.56. 1H NMR (CDCl3, 270 MHz) 6 5.31 (t, 1H, J=6.2 Hz), 5.09 (m, 2H), 4.76 (m, 1H), 4.15 (d, 2H, J=6.2 Hz), 3.71 (d, 2H, J=7.62 Hz), 1.9-2.1 (m, 10H), 1.63 15 (s, 3H), 1.60 (s, 3H), 1.59 (s, 3H), 1.35 (d, 6H, J=5.87 Hz), 1.02 (t, 3H, J=7.3 Hz) ppm.

C. (2Z,6E)-[[[[(3-Ethyl-7,10-dimethyl-2,6,10-dodecatrienyl)oxy]methyl](1-methvl-20 ethoxy)phosphinyl]methyl]phosphonic acid, dimethyl ester A solution of 250 mg (0.67 mmol) of Part B triester in 3.5 mL of dry CH2C12 at room temperature under argon was treated with 255 (jL (1.34 mmol, 25 2.0 equiv) of N,N-diethyltrimethylsilylamine and stirred for 1.5 hours. The solvent was evaporated, the residue re-evaporated from benzene and dried at high vacuum for 0.5 hour. The residue was dissolved in 3.5 mL of dry CH2C12 and treated at 30 0°C under nitrogen with one drop of dimethylformamide and dropwise with a solution of 650 pL (1.34 mmol, 2.0 equiv) of 2 M oxalyl chloride in CH2C12. The reaction was stirred at room temperature for 2 4 4 3 6 3 two hours, the solvent was evaporated, and the residue was evaporated from benzene ana dried at high vacuum for 0.5 hour.

The anion solution was prepared by treating 5 a solution of 160 pL (1.47 mmol, 2.2 equiv) of dimethyl methylphosphonate in 3 mL of tetrahydrofuran at -78°C under argon dropwise with a solution of 0.90 mL (1.44 mmol, 2.15 equiv) of 1.5 M n-butyllithium in hexane and stirring for 0.5 hour. A 10 solution of the phosphonic acid chloride prepared above in 2 mL of tetrahydrofuran was added dropwise. The reaction was stirred for two hours at -78°C, quenched with NH^Cl, diluted with 5 mL of CH2Cl2 and warmed to room temperature. Additional 50 mL 15 of CH2C12, 5 mL of H70 and 5 mL of 1 M HCl were added and the layers separated. The aqueous phase was extracted with 50 mL CH2C12. The combined organic layers were washed with brine, dried over MgSO^, and evaporated to give 331 mg of crude 20 product as a yellow oil. Purification by flash chromatography on 35 g silica gel, eluted with 1.5:98.5 CH30H:CH2C12, provided 170 mg (53%) of pure title product as a light yellow oil.

TLC Silica gel (5:95 CH30H:CH2C12) Rf=0.33 IR (CC14) 2967, 2929, 2877, 1451, 1257, 1230, 1180, 1165, 1105, 1090, 1063, 1036, 992, 841, 824, 815, cm"1. 1H NMR (CDC13, 270 MHZ) 5 5.22 (t, 1H, J=7.04 Hz) 5.02 (m, 2H), 4.73 (m, 1H), 4.06 (d, 2H, J=7.04 Hz), 3.75 (d, 3H, J=ll.l Hz), 3.73 (d, 3H, J=ll.l O / / 7^7 L t '! 0 r; ,5 Hz), 3.6-3.9 (m, 2H), 2.2-2.6 (m, 2H), 1.3-2.1 (m, 10H), 1.60 (s, 3H), 1.52 (s, 6H), 1.28 (t, 6H, J=6.8 Hz), 0.95 (t, 3H, J=7.3 Hz) ppm.

Mass Spec (CI-CH4/N20, - ions) m/e 477 /M-H) Example 32 (22,6E)-[[[[(3-Ethvl-7,10-dimethyl-2,6,10-dodeca-trienyl)oxy]methyl]hydroxyphosphinyl]methyl]phos- phonic acid, trisodium salt A solution of 165 mg (0.34 mmol) of Example 31 triester in 2 mL of dry C^Cl^ was treated with 135 pL (1.02 mmol, 3 equiv) of 2,4,6-colliaine and 270 pL (2.04 mmol, 6 equiv) of bromotrimethylsilane 15 and stirred for 24 hours. The solvent was evaporated and the residue was treated with 1.05 mL (1.05 mmol, 3 equiv) of 1 M NaOH to pH 14, and lyophilized. Purification was by chromatography on a 2.5 cm diameter x 18 cm height column of 20 CHP20P resin packed in water and eluted with 100 mL of water followed by a gradient created by the gradual addition of 300 mL of CH^CN into 300 mL of water. Approximately 8 mL fractions were collected every 1.3 minutes. Fractions 41-50 were 25 combined, evaporated, lyophilized and dried at high vacuum overnight to obtain 90 mg (56%) of title product as a white lyophilate.

TLC Silica gel (4:4:1 n-C^OH:con NH3:H20) Rf=0.63 IR (KBr) 3437, 3053, 2924, 2876, 1652, 1192, 1133, 1106, 1047, 899, 864, 766 cm"1. 0 /. /. 7 c h ^ o T •172- LH NMR (D20, 400 MHz) 6 5.36 (t, 1H, J=7 Hz), 5.13 (m, 2H), 4.11 (d, 2H, J=7 Hz), 3.62 (d, 2H, J=6.2 Hz), 1.9-2.2 (m, 12H), 1.63 (s, 3H), 1.55, 1.56 (two s 6H), 0.96 (t, 3H, J=7 Hz) ppm Mass Spec (FAB, + ions) m/e 497 (M+Na), 475 (M+H) Anal Calcd for CigH32P206Na3 x 0.30 mol H90: C, 45.07; H, 6.64; P, 12.91 10 Found: C, 45.12; H, 6.81; P, 12.77 Example 33 (E)-[[[[(8,12-Dimethyl-7,li-tridecadien-3-ynyl)oxy]-methyl](1-methylethoxv)phosphinyl]methyl]phosphonic acid, dimethyl ester A. (E)-8,12-Dimethyl-7,ll-tridecaaien-3- yn-l-ol A solution of 602.3 mg (3.40 mmol) of Example 16, Part A acetylene in 15 mL of tetrahydro-20 furan at -78°C under argon was treated dropwise over 5 minutes with 2.35 mL (3.75 mmol, 1.1 equiv) of a 1.6 M solution of n-butyllithium in hexanes and stirred for 0.5 hour. To this yellow solution was rapidly added 530 jjL (6.8 mmol, 2.0 25 equiv) of a 12.8 M solution of ethylene oxide in tetrahydrofuran which had been cooled to -78°C, followed by 460 pL (3.75 mmol, 1.1 equiv) of boron trifluoride etherate. The reaction was stirred for three hours at -78°C, quenched with saturated 30 NHjCl and diluted with 800 mL of diethyl ether.

The organic phase was washed with water and brine, dried over MgSO^ and evaporated to give 753.4 mg .? 4 L \ P. J 1 a W i%l. of crude product. Purification by flash chromatography on 75 g of silica gel, eluted with 1:9 ethyl acetate: hexane gave 476.6 mg (54%) of the desired title product as a clear, colorless oil. 5 In addition, 204.4 mg (34%) of Example 1, Part A acetylene was recovered.

TLC Silica gel (2:8 ethyl acetate: hexane) R^=0.36 IR (CC14) 3634, 3590, 2966, 2915, 2883, 2855, 1444, 1434, 1383, 1378, 1329, 1184, 1108, 1053, 850, 830, 810, 790, 780, 760, 740 cm"1. 1H NMR (CDClg, 270 MHz) 6 5.16 (m, IK), 5.10 (dt, 15 1H, J=1.17), 3.66 (t, 2H, J=6.45 ppm), 2.42 (t, 2H, J=6.45 ppm), 2.18 (br, 4H), 2.0-2.1 (m, 5H), 1.68 (s, 3H), 1.62 (s, 3H), 1.60 (s, 3H) ppm.

Mass Spec (CI-NH3, + ions) m/e 238 (M+NH4), 221 20 (M+H) B. (E)—[[(8,12-Dimethyl-7,ll-tridecadien-3-ynyl)oxy]methyl]phosphonic acid, bis(1-methylethyl) ester A solution of 450.7 mg (2.04 mmol) of Part A alcohol in 5 mL of tetrahydrofuran at -78°C under argon was treated over five minutes with 1.35 mL (2.14 mmol, 1.05 eguiv) of 1.6 M n-butyllithium in hexanes and stirred for 0.5 hour. A 30 solution of 702 mg (2.14 mmol, 1.05 equiv) of Example 1, Part B triflate in 2 mL of tetrahydrofuran was added via cannula. After 45 minutes at -78°C, the reaction was allowed to warm to 0°C 2 4 4 3 ft gradually over one hour, then stirred at 0°C for four hours. The reaction was quenched with NH^Cl and diluted with diethyl ether. The organic phase was washed with water and brine, dried over MgSO^, 5 and evaporated to yield 793.7 mg of crude product. Purification by flash chromatography on 70 g of silica gel, eluted with 3:7 ethyl acetate: hexane, provided 643.1 mg (79%) of title diester as a clear, colorless oil.

TLC Silica gel (3:7 ethyl acetate: hexane) R^=0.17 IR (CC14) 2980, 2929, 2885, 1449, 1436, 1385, 1375, 1258, 1242, 1141, 1107, 1007, 991, 906 cm"1. 1H NMR (CDC13, 270 MHz) 5 5.15 (m, 1H), 5.10 (dt, 1H, J=1.17, 7.04 Hz), 4.76 (m, 2H) 3.76 (d, 2H, J=8.80 HZ), 3.65 (t, 2H, J=7.3 Hz), 2.45 (tt, 2H, J=2.3, 7.3 Hz), 1.9-2.2 (m, 8H), 1.68 (s, 3H), 20 1.60 (s, 6H), 1.34 (d, 12H, J=5.9 Hz) ppm.

Mass Spec (CI-NH^, + ions) m/e 416 (M+NH^), 399 (M+H), 202, 69 C. (E)-[[(8,12-Dimethyl-7,11-tridecadien- 3-ynyl)oxy]methyl]phosphonic acid, mono(1-methylethyl) ester A mixture of 621.3 mg (1.56 mmol) of Part B diester, 15.6 mL (15.6 mmol, 10 equiv) of 1 M KOH 30 and 16 mL of 2-propanol was stirred under nitrogen at 90°C for 24 hours. After cooling, the 2-propanol was evaporated and the residue was diluted with 50 mL of CH2C12 and acidified to pH 1 with 10% HCl. 2 4 4 3 The aqueous phase was re-extracted with 50 mL of CH2C12, and the combined organic layers were washed with brine, dried over MgS04 and evaporated to afford 559.2 mg (100%) of title phosphonic acid 5 as a clear, colorless oil.

TLC silica gel (8:1:1 n-C-^OH: con NH3:H20) Rf=0.52 NMR (CDC13, 270 MHz) 6 11.17 (br s, 1H), 5.12 (m, 10 2H), 4.75 (m, 1H), 3.79 (d, 2H, J=8.79 Hz), 3.56 (t, 2H, J=7.3 Hz), 2.45 (t, 2H, J=7.3 Hz), 1.9-2.2 (m, 8H), 1.68 (s, 3H), 1.60 (s, 6H), 1.35 (d, 6H) ppm.

D. (E)-[[[[(8,l2-Dimethyl-7,11-tridecadien- 3-ynyl)oxy]methyl](1-methylethoxy)phosphinyl]- methyl]phosphonic acid, dimethyl ester To a solution of 553.8 mg (1.55 mmol) of Part C phosphonic acid in 10 mL of CH2C12 at room temperature 20 under argon was added 590 yL (3.10 mmol, 2 equiv) of N,N-diethyl(trimethylsilyl)amine. After stirring for 1.5 hours, the solvent was evaporated and the residue was evaporated from benzene and dried at high-vacuum for 30 minutes. The residue was dissolved in 10 mL 25 of CH2C12 and one drop of dimethylformamide at 0°C under nitrogen. The solution was treated with 1.40 mL (2.8 mmol, 1.8 equiv) of a 2.0 M solution of oxalyl chloride in CH2C12 over five minutes.

After stirring for three hours, the solvent was 30 evaporated, and the residue was evaporated from benzene and dried at high vacuum for 30 minutes.

The anion solution was prepared by treating a solution of 370 pL (3.45 mmol, 2.2 equiv) of 0 / / \ £: v >.J \ L 4 dimethyl methylphosphonate in 10 mL of tetrahydrofuran at -78°C under argon with 2.10 mL (3.35 mmol, 2.15 equiv) of 1.6 M n-butyllithium in hexanes over ten minutes. After 30 minutes, a 5 solution of the phosphonic acid chloride prepared above in 5 mL of tetrahydrofuran was added dropwise over ten minutes, and the resulting solution was stirred for 1.5 hours at -78°C. After quenching with saturated NH^Cl and diluting with the mixture was allowed to warm to room temperture, then treated with H20 and 1 M HCl and separated. The aqueous phase was re-extracted with two portions of CH2C12. The combined organic layers were washed with brine, dried over MgS04 and evaporated 15 to give 802.0 mg of an orange oil. The crude product was purified by chromatography on silica gel, eluted with 2:98 CH3OH:CH2Cl2 to obtain 331.2 mg (46%) of title triester as a yellow oil.

TLC Silica gel (5:95 CH3OH:CH2Cl2) Rf=0.46 IR (CC14) 2977, 2954, 2919, 2875, 2853, 1450, 1436, 1385, 1375, 1256, 1231, 1180, 1166, 1140, 1109, 1064, 1036, 993, 842, 820 cm"1 1H NMR (CDC13, 270 MHz) 5 5.12 (m, 2H), 4.80 (m, 1H, ), 3.90 (m, 2H), 3.83 (d, 3H, J=10.8 Hz), 3.80 (d, 3H, J=10.8 Hz), 3.66 (td, 2H, J=7.0 and 2.0 Hz), 2.3-2.7 (m, 4H), 1.9-2.2 (ra, 8H), 1.68 (s, 3H), 30 1.61 (s, 6H), 1.37 (d, 3H, J=6.45 Hz), 1.35 (d, 3H, J=6.45 Hz) ppm.

ILL 7 ft \ w f i '■_/ \j Mass Spec (CI-CH^/^O, + ions) m/e 491 (M+C2H,-), 463 (M+H), 421.

Example 34 (E)-[[[[(8,12-Dimethyl-7,ll-tridecadien-3-ynyl)-oxy]methyl]hydroxyphosphinyl]methyl]pho sphonic acid, trisodium salt A solution of 363 mg (0.78 mmol) of Example 33 triester in 5 mL of CE^Cl^ at room temperture 10 under argon was treated with 310 jjL (2.35 rnmol, 3 equiv) of 2,4,6-collidine and 620 pL (4.68 mmol, 6 equiv) of bromotrimethylsilane and stirred overnight. The solvent was evaporated and the residue was basified to pH 14 with 2.35 mL of 1 M NaOH. Purifi-15 cation was by chromatography on a 2.5 cm diameter x 20 cm height column on CHP20P gel eluted with 100 mL of water, followed by a gradient created by the gradual addition of 400 mL of acetonitrile into 400 mL of water. Approximately 10 mL 20 fractions were collected every 1.5 minutes.

Fractions 31-40 were combined, evaporated, lyophilized, and dried at high vacuum to obtain 255 mg (70%) of title salt as a white lyophilate.

TLC silica gel (4:4:1 n-C^OH: con NH3:H20) Rf=0.41 IR (KBr) 3453 (br), 2967, 2917, 1666, 1437, 1179, 1149, 1099, 998 cm"1. "hi NMR (D20, 400 MHz) 6 5.19, 5.15 (two t, 1H each) 3.68 (d, 2H, J=6.2 Hz), 3.62 (t, 2H, J=6.6 Hz), 2.42 (t, 25, J=6.6 Hz), 2.14 (m, 4H), 2.07 (q, 2H, / -i / ivi J=7 Hz), 1.99 (t, 2H, J=7 Hz), 1.92 (t, 2H, J=13 Hz), 1.63 (s, 3H), 1.58, 1.57 (two s, 3H each) ppm.

Mass Spec (FAB, + ions) m/e 481 (M+Na), 459 (M+H), 5 437 (M+2H-Na).

Anal Calc'd for C^7H2~?2°6Na3*1mo1 H20: C, 42.10; H, 6.23; P, 12.77 Found: C, 41.91; H, 6.15; P, 12.93 (E,E)-[[(3,7,ll-Trimethyl-2,6,10-dodecatrienyl)- oxy]methyl1phosphonic acid, diethyl ester Following the procedure of Example 1, Part 15 C (1) except substituting the Example 5 Part A carbinol for the carbinol employed in Example 1, Part C (1), the title compound is obtained.

A. (E,E)-l-Chloro-3,7,11-trimethyl- 2,6,10-dodecatriene (Note: all temperatures indicated are for the contents of the reaction flask). To a stirred 25 solution of 299 mg (2.24 mmol) of N-chlorosuccinimide in 15 ml of dichloromethane at -30°C under argon was added 0.18 ml (2.45 mmol) of distilled dimethyl sulfide over 5 minutes. After 10 minutes at -30°C, the reaction was allowed to 30 warm to 0°C for 10 minutes, followed by cooling to -40°C. A solution of 441.4 mg (1.99 mmol) of 3,7, ll-trimethyl-2,6,10-tridecatrien-l-ol in 5 ml of dichloromethane was added dropwise over 10 Example 35 Example 36 6,10,14-Trimethyl-5,9,13-pentadeca-trien-l-ol minutes. The reaction was allowed to warm gradually to 0°C over 1 hour, and then maintained at 0°C for 1 hour. After quenching with cold water, the mixture was extracted with hexane and the hexane extract was washed with cold water and cold brine, dried (MgSOd) and evaporated to afford 483 mg of a crude product. Rapid flash chromatography on 20 g of silica gel eluted with 3:97 ethyl acetate:pet ether provided 406.5 mg (85%) of a 13 colorless liquid. C NMR indicated that this material contained a trace (3%) impurity.

TLC:Silica gel (2:98 ethyl acetate:hexane) Rf=0.56 1H NMR(CDC13) (270 MHz) 6 5.44 (t, 1, J=7.9 Hz) 5.09 (t, 2, J=5.8 Hz) 4.07 (d, 2, J=7.9 Hz) 1.9-2.2 (m, 9) 1.72 (s, 3) 1.68 (s, 3) 1.60 (s, 6) ppm. 3 1 B. Dichloro[mu-[1-propanolato(2-)-C :0 ]]-dimaanesium A modification of the procedure of G. 25 Cahiez et al. was employed (Tetrahedron Letters, 1978, 3013-4): To a stirred solution of 1.89 g (20 mmol) of 3-chloropropanol in 20 ml of THF under argon at -20°C was added 10 ml (20 mmol) of 2 M phenylmagnesium chloride in THF over 15 30 minutes. After 10 minutes at -20°C, the reaction was allowed to warm to room temperature, 730 mg (30 mmol) of magnesium turnings were added and the 9 L h X G 7 C~ **f -J ■J -3 reaction was heated to reflux. Two 40 pi portions of 1,2-dibromoethane were added, the first portion injected at the start of reflux, and the second after 1 hour. After refluxing for a total of 2 5 hours, the reaction was allowed to cool to room temperature.

C. (E,E)-6,10,14-Trimethyl-5,9,13-pentadeca- trien-l-ol A solution of 37.5 mL (20.3 mmol, 5.1 eq. ) of a 0.54 M solution of Grignard reagent (Part B) in tetrahydrofuran and 9 mL of hexamethylphosphor-amide at room temperature under argon was treated over 10 minutes with a solution of 955.5 mg (3.97 15 mmol) of (E,E)-farnesyl chloride (Part A) in 5 mL of tetrahydrofuran. After one hour, the reaction mixture was diluted with a mixture of 1:1 diethyl ether : hexane and quenched with 1 M HCl. The organic phase was washed with three 25 mL portions 20 of saturated NaHCO^, three 25 mL portions of H20, and 25 mL of brine, dried over MgSO^ and evaporated to obtain 995.0 mg of crude product. Purification required two chromatographies. The first was run on 70 g of silica gel, eluting with 25 1:99 ethyl acetate:CH2C12 to provide 484.3 mg of impure material and 307.7 mg of pure title compound. The second chromatography, of the impure fractions, on 50 g of silica gel eluted with 0.75:99.25 ethyl acetate:CH2C12 gave 117.2 mg of slightly impure 30 material and 302.8 mg of pure title compound. Combination of pure material from both columns gave a yield a yield of 610.5 mg (58%) of pure desired title isomer.

A /; T C T ^ r <J 0 J TLCrSilia gel (10:90 ethyl ether:CH2C12) Rf=0.38 IR (CC14) 3639, 3450, 2964, 2930, 2858, 1449, 1382, 1058, 1028, 776, 750 cm"1.

XH NMR (CDC13) (270 MHz) 6 5.10 (m, 3H) 3.62 (t, 2H, J=6.5 Hz) 2.00 (m, 10H) 1.69 (s, 3H) 1.61 (s, 9H) 1.2-1.7 (m, 5H, OH) ppm.

Mass Spec (CI-CH4/N20, + ions) m/e 282 (M + NH^), 265 (M+H), 263 (m + H-H2).

O /. / 7 -? £ ; h ^ Examples 37 to 110 Following procedures of Examples 1 to 36, the following additional compounds may be prepared in accordance with the present invention. It will be appreciated that the compounds listed include all stereoisomers thereof.

H HH 0 Y1 0 CH_-C=C-CC-C=CH-CH0-Q^- (CH- ) -X-(CH0 )_-P C—P-OR3 3 i mi 2 2 n 2 m i 7 i _ i .

CH3 HH CH3 0RZ Y OR Ex. 4 1 2 3 2 4 No. Q X n m L L £ £ L CH-CH-- // 2 37. -CH -C 0 23HHKKK 1 i ch3 38. bond 0 0 1 F F Na Na Na CH-CH.-// 2 39. -CH -C 0 1 1 Cl Cl Na Na Na ^ i ch3 CH-CH-- // 2 40. -CH -C 0 0 1 H Cl Na Na Na i C"3 v 41. -fCH2-C 0 0 1 H H K K K iH3 12 CH-CH.-// <■ 42. -CH„-C -NH- 0 1 H F CH0 K K l CH„ 2 T 3 3 CH-CH.-/J' 2 43. -CH.-C -NCH-- 0 1 H H Na Na Na 2 | 3 ch3 /;h-°h2- 44. -CH -C 0 0 1 H F K K K 2 , CH. 3 24 4 3 6 3 Ex.

No. n m Y1 Y2 R3 R2 R4 45. 46. 47. 48. 49. 50. 51. 52.

/ CH-CH.

L * 2 'CH.-C ^ i CH.

CH-CH.- '/ 2 -CH.-C 0 i CH3 CH-CH,- -CH -C -NH- *• i CH3 CH-CH,- // 2 -CH.-C 0 *■ i CH3 CH-CH.V X/ 2 \ LCH.-C * i CH i // CH 3 CH-CH, ■CH -C 2 i bond 0 CH-CH. // 2 C i CH.

-CH,-C NH ^ i 3 3 F K H -Mg- 0 1 Cl Cl K K K 1 1 H H K K K 2 1 F F Na Na Na 0 1 H F K K K 1 1 F F K K K 0 2 H H Na Na Na 1 1 F F K K K 53- bond NH 1 1 H H Na Na Na • • A R7 ch- ^ ch ch- ch ch 3 ^ n / 2 4, \ / c ch_ c ch~ R8 V A ch, i ch, c I R, ch, 0 (CH2)n-X-(CH2)m-.p y1 o I 11 3 -C—P-OR 2 1 2 1 4 or y or No.

RZ si ISO 1 00 n X m Y^ x£ E?.

R3 R4 54 h i h 0 o 1 f f K K K 55 h h I 1 0 1 H h Na Na Na 56 h ch3 ch3 0 o 1 f h ( Mg ) ch 57 ch3s ch3 h 1 o 1 h H K K K 58 f ch3 H 0 s 1 H H Na Na Na 59 ch3 ch3 h 1 o 1 h h K K K 60 h ch3 ch3 1 0 1 H h Na Na Na 61 h ch3 cl 0 o 1 h h K K K 62 h <*3 h 0 o 1 H h K K K 63 h cl h 1 o 1 H h K K K 64 h CH3 (CH3)3Si 0 o 1 H H Na Na Na 65 H ch_ F 1 o 1 f H K K K oo i ro 4>» • § •AO ^ n / 2 \ // \ / 2n<^ \ x c ch0 c ch~ c ch I 2 I 2 I RX1 CH3 CH3 ex.

No. r10 r11 rx 66 c2h5 ch3 0 67 ch3 c2h5 1 68 n-c3h7 ch3 1 69 ch3 n~c3h7 0 70 ch3 n-C4H 0 71 t-^hg ch3 0 72 -<ch2 »5" 0 73 h h 0 74 f f 0 75 cl cl 1 76 ch2f ch3 0 77 -ch=ch2 h 1 o (ch2)n-x-(ch2)m-p- or Y O I h q -C—P-OR 2i2 iR4 X m Y^ x! R3 0 1 h h ch3 K K 0 1 Cl Cl Na Na Na 0 1 h h f~ Mg —) h 0 1 Cl h K K K 0 1 h h K ch3 K 0 1 f h K K ch 0 1 h 11 K K K 0 1 h h Na Na Na 0 1 h h r Mg \ h 0 1 h h K K K 0 1 h h Na Na Na 0 1 h h K K K o> CM ** / /- X £ "Z ch h o o 0 186- I <o Kl X 2 <C a: 2 ^ ^ cn <0 u oiifizp u:u:z;<:a: CM al x 2 •L CT> E en X « o ^ z ^ u: rn Ci 0 <* 1 « 0 = fc-0 -t I <\J I N I PS Orflu-O I ^_E eg E 0 1 X I cn i >■•1 X E E X X X ><|EEEEEEEE CM s u XIOOOOOOOO CM E o / E u ^ vD U-DS CM, E ' U \e" a E ^ U ^ vjD cj-OS °v E / U \ ^ N E U E ' U ^ 2 a-os x/ a ClOrHO OOr-lOO kO os vO OS ui in in rn E E E co m E cm cm cm S3 E U U U U O O E in in in in E m E E E m CM E CM CM CM E UCJUUUEUE in in E <n n E n en en (ME X cm E E E UOUUCJUUE X o « Z! aooHNn<#in c^r-cocococoaoco 24 4 3 6 3 Ex. No. 86 OHO CH. CH, 1 CH, CH, \CH, CH, O-CH,-P-C—P-OK \3 / ',2 > 2W 2\ / 2W 2 / 2 i i t CH CH,. CH CH,. CH CH, OK H OK i ^ t — \ i z CH. 3 CH. 12 C«3 87 CH \3 / CH I CH- CH.

CH, 0 h 0 h CH, CH, CH, CH, CH \2 / 2\ / 2 / 2 ^ s ✓ CH, CH CH, C CH, Z i Z | z .0-CH,-P—C—P-OK 2 . I I OK F OK 88 CH \- 0 H 0 II ! II CH CH, CH, CH, CH CH,-0-CH,-P—C—P-ONa ^'\/2^/n2/\2<^N/2 2I II C CH, CH CH, C CH, ONa H ONa i — i ^ i *■ CH3 CH3 CH3 OHO II | M 89 CH, CH CH, CH CH,-C=C-CH,-N-CH,-P—C—P-OK / 2 2 | 2 | | i C CH, C CH, H i Z ( z CH, CH, 2 i i OK H OK 90 .CH, CH, .CH CH, CH / si / 2\ // \ / sL jy \ CH. CH, CH CH, C 3 2 2 | CH- 0 H 0 II i II ^ 0-CH,-P—C—P-OK / 2 | | i CH2 OK H OK CH, CH- OHO i 3 i 3 ll i H 91 CH, CH CH CH CH CH 0-CH--P—C—P-OK \3 ^ \ / \ // \ / \ # \ / 2i i l C CH- C CH_ C CH, OK H OK CH- CH, i CH, 9 A A X ft L *? "f ^ 0 92 0 H 0 ch. ch yj // c ch3 / ch- CH- CH «£\ /, \ C i Cl / CH, CH- CH O-CH.-P C—P-ONa -V' v 'I C CH. ONa H ONa i *■ ch3 ch3 oho 93 ^ch ch. c ch. ch o-ch,—p—c—p-ok 'y \ / 2\^\ysl^\/ 2 , , ch- ch. c ch, c ch, ok h ok Z 2 | 2 i 2 ch- i 94 ch, v ch V x c c2h5 CH- OHO i j n i '.i ch_ ch , c ch ch,-0-ch,-p—c—p-ok / T-s /, s / \ /, s / 2 2 i , , ch, CH„ CH. c i choi.

OK H OK ch, 0 f 0 i 3 11 i M 95 / CH CH. C CH, CH 0-CH--P—C—P-OK ^ \ s is ^ \ / i\ // \ y 2, , , CH. CH, C CH, C CH, OK F OK L Z | Z | L ch. ch. fH3 96 ch, c ch C ch. i *■ CH3 2v * c i ch. ch \ / * v2 # \ / | £. | I , ch, v-ch, c * i ch, ch OHO ii i H n-ch.-p—c—p-ok ch2 h OK H OK 97 /y CH CH ch ch c2h5 CH, c l ch.

CH2 \z CH, i J C O-CH.-P 1-\ // \ / // \ / 2 i CH„ CH ch.

OHO il i il c—P-ONa i l ONa H ONa 244363 t 98 ch- ch ch. \3 ^ \ / 2, c ch, I 2 c2h5 ch / ch.

CH. fH3 CH N ^ c I CH- ch 0 ii h CH2 0-CH--P 2 i C—P-ONa i i ONa H ONa 99 ch- ch ch. ch \3 v / \2 v c ch- c ch, i *■ , £■ / C"2 <?• CH CH- OHO i J ii i 'i C CH,-O-CH.-P—C—P-OK s y 2 2 , , , CH„ OK H OK ch. ch. 100 CH- CH „ \3 ^ X / C CH, i i- ffl3 f 0 cl CH. C CH, CH CH,-0-CH,-P—C— C CH, C CH- OK Cl i 2 I 2 ch.

CH. 0 li -P-OK OK F 101 ch- c ch- ch \3 ^ \ \ CH, CH \ / \2 \ / C CH, C CH, C CH, I 2 i 2 | 2 CH_ CH_ CH„ OHO II i I' 0-CH,-P—C—P-OK * i ii OK H OK 102 CH CH.

CH, ch, CH, ch, 0 ii 0 it \3 ys 2\ / 2S / 2\ y 2\ / 2 \ \ / j 2 CH CH„ CH CH„ CH CH- CH_ ONa P-CH„-P-ONa CH, i ch, ONa i ch, 103 104 0 il 0 CH, CH 0 . P—CH — P-ONa \J ^ \ / \ -* i 2 i n c ch. ch, ONa ONa I 2 2 cii3 0 II ch, ch, V ' 2\ n ch ch, I 2 ch3 ch- OK 0 H 0 . P—CH,—P-OK / \ / i 2 | OK 24 4 3 6 3 o o ii it 105 CH_-ChC-CH_0-CH--P—CH,—P—ONa 3 2 2,2, ONa ONa 106 CH3 X CH C ca3 CH, / CH, \ ch. ch 0 ch2 c ch2 ch2 ch3 0 II 0 II / ■ OK p—ch —p-ok OK /CH2 107 CH3-chc xch2 CH_ CH- 0 0 i 3 i 3 II il . C CH. C CH- CH.—P-CH--P-ONa / \\ / A / \\ / 2V / 2 | 2 | CH CH CH 0 ONa ONa 108 CH3-CHC ch, i - ch. c / \2 / v\ CH- CH CH, CH, ^ s2 / 2 0 \M 0 II P—CH_—P—OK i 2 I OK OK ch- ch ch, \3//\ / 2s 109 c ch.

I 2 ch3 v u , \ / \ / CH- CH„ ONa 0 0 II M P—CH.—P—ONa ONa 110 <*3 CH / 2\ CH l CH- CH, CH, / 2\ / 2\ 0 / \ / 0 0 II II p—ch-—p—ok CH, CH i CH, CH_ CH- OK OK I * : :■ f

Claims (13)

1. WHAT WE CLAIM IS: lm A compound having the structure 11 2a R -(CH-) -X -(CH-) -P-OR 2 n 2 m | - OR R1-(CH2)n-X1-(CH2)m-P-0-(5y 0 NO- 0 1 1 11 R-(CH-) -X -(CH-) -P-OH or 2 n 2 m i n ORZa 0 ^-(CHaln-x'-IOTzl^P-C1 OR2a wherein n is 0, 1, 2 or 3; m is 1, 2 or 3; X is 0, S, NH or N-Pro; XI is 0, S or N-Pro and Pro is a nitrogen 2 . protecting group; X is 0 or S; 2a 1 R is C^-CQalkyl or C3_C^-alkenyl; and R is R^-Q^-Q^-Q3- wherein Q , Q and Q3 are independently: R7 R6 R8 R9 ill i -CH—-C=C-CH2-, -CH-CH-CH2-CH2", -CH2-C=C-CH2- or a bond, with the stipulation that if Q1 is a 2 3 2 bond, then Q and Q must be bonds, and if Q is a •3 £ bond, then Q is a bond; R is H, lower alkyl, O halo or haloalky.1; R is H, halogen, trimethylsilyl 7 or lower alkyl; R is H, halogen, lower alkyl or Q lower alkylthio; R is H or lower alkyl; and 16 192- V 3 3 RnR12 5 . 10 1 1 R is R -C=C-CH2- , R13 14 1 R -C-CH--CH-- , 1 fi R -C=C-CH2- or CH_(CH-) - where p is 2 to 7; 10 11 P R and R are independently H, lower alkyl, halogen, lower alkenyl or haloalkyl; or R10 and R^ can be taken together to form (CH-) where s is 2 12 to 7; R is H, lower alkyl, halogen or lower 13 14 alkenyl; R and R are independently lower alkyl; and R^ is lower alkyl or H; with the proviso that if all of Q"1", Q2 and Q3 are bonds then R10 and R^ cannot be H, and R5 cannot be CH3(CH2)p- where p is less than or equal to 4.
2. 2. A compound as defined in Claim 1 having the structure q ,1 / /-.T-r \ -.r / nrl \ _Ti_nD2a R -(CH- ) -X -(CH-) -P-OR v 2'n ' 2'm i - OR wherein n, m, X, R2a and R1 are as defined in Claim 1.
3. 3. A compound as defined in Claim 2 wherein Z is NH or N-Pro.
4. 4. A compound as defined in Claim 1 having the structure q R1- (CH2 Jh-X1- (CH2 )m-P-0-/^\-N02 OR N ' wherein n, m, X1, R2a and R1 are as defined in Claim 1.
5. 5. A compound as defined in Claim 1 t.., having the structure q Rl-<CH2>n-xl-<CH2>m"?-°? OR wherein n, m, x1, R2a and R1 are as defined in Claim 1. •. i '' ) —, / 1 ,2a -193- 24, V
6. 6. A compound as defined in Claim 1 having the structure Q ,2a Rl-(CH2)n-X2-(CH2)m-P-Cl OR wherein n, m, X2, R2a and R1 are as defined in Claim 1 .
7. 7. The compound having the name [[[(trifluoro-methyl)sulfonyl]oxy]methyl]phosphonic acid, bis(l- methylethylJester.
8. 8. A method for preparing a compound of the formula 0 R1-(CH_) -X1-(CH-) -P-OH 2 n 2 m | _ OR as defined in Claim 5, which comprises treating a diester of the formula 0 R1-(CH-) -XX-(CH,) -P-OR2a 2 n 2 m | , OR wherein n, m, X*, R^a and R^ are as defined in Claim 1, with a strong aqueous base, an alkali metal cyanide or an alkali metal halide.
9. 9. A method for preparing a monoester of the formula 0 1 1 11 R-{CH-) -X -(CH-) -P-OH 2 n 2 m i - OR as defined in Claim 5 wherein XX is 0 or S, which'' r comprises treating a diester of the formula A 0 Rl-<CH2>n-xl-<CH2>m-f-°fa "' ■ '// 0R2a • . .. •. wherein X-*- is 0 or S and n, m, R^a and R^ are as defined in Claim 1, with bromotrimethylsilane or iodotrimethyl-silane, under an inert atmosphere, in the presence of 2,4,6-collidine, and then treating with an alcohol of the formula R^aOH and dicyclohexylcarbodimide to form the monoester product. -194- 24 a p. T
10. 10. The method for preparing a compound a% v v/ defined in Claim 1 having the formula O r1-(ch_)n~x^ -(ch-) -p-or2a 2 m | . or wherein n, m, X \ R2a and R1 are as defined in Claim 1, which comprises treating a solution of a compound of the formula O R1-(CH2)n-X1H with Rh2(0CCH3)4 wherein n, X1 and R1 are as defined in Claim 1, and then with a diazophosphonate of the formula 0 11 2a n-ch-p-or 2 1 2a or wherein is as defined in Claim 1.
11. 11. The method for preparing a diester as defined in Claim 1 having the formula O R ~(CH2 " 2a 2 m ' 2a or wherein n, m, r2s an<3 R1 are as defined in Claim 1 and X is 0, S or NH, which comprises treating a compound of the formula r1-(ch2)n-xh wherein X is 0, S or NH and n and R1 are as defined in Claim 1, with a base and then with a phosphonate of the formula 0 ,2a z-(ch_) -p-0r2a 2'm t or wherein Z is p-CH3CgH4S03~ or CF3S03~and m and R^a are as defined in Claim 1, under an inert atmosphere^f^ \ to form said diester. ii -195- 24 -v4 7 y <J
12. 12. The method as defined in Claim 11 wherein said base is n-butyllithium, sodium hydride or ((C^^-Si^NLi and said reaction is carried out at a temperature within the range of from -78°C to 25°C, when X is 0 or S.
13. 13. The method as defined in Claim 11 when said base is trialkylamine and said reaction is carried out at a temperature within the range of from -20°C to 80°C when X is NH. JATi t