NZ242694A - Site specific nucleases consisting of a fusion protein of a ligand-nuclease hybrid and their use - Google Patents

Site specific nucleases consisting of a fusion protein of a ligand-nuclease hybrid and their use

Info

Publication number
NZ242694A
NZ242694A NZ242694A NZ24269492A NZ242694A NZ 242694 A NZ242694 A NZ 242694A NZ 242694 A NZ242694 A NZ 242694A NZ 24269492 A NZ24269492 A NZ 24269492A NZ 242694 A NZ242694 A NZ 242694A
Authority
NZ
New Zealand
Prior art keywords
nuclease
ligand
hybrid
fusion protein
site specific
Prior art date
Application number
NZ242694A
Other languages
English (en)
Inventor
Chang Yie-Hwa
John A Smith
Original Assignee
Gen Hospital Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Gen Hospital Corp filed Critical Gen Hospital Corp
Publication of NZ242694A publication Critical patent/NZ242694A/xx

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • C07K2319/22—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a Strep-tag
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/35—Nature of the modification
    • C12N2310/351—Conjugate
    • C12N2310/3513—Protein; Peptide

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
NZ242694A 1991-06-11 1992-05-12 Site specific nucleases consisting of a fusion protein of a ligand-nuclease hybrid and their use NZ242694A (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US71290591A 1991-06-11 1991-06-11

Publications (1)

Publication Number Publication Date
NZ242694A true NZ242694A (en) 1993-07-27

Family

ID=24864017

Family Applications (1)

Application Number Title Priority Date Filing Date
NZ242694A NZ242694A (en) 1991-06-11 1992-05-12 Site specific nucleases consisting of a fusion protein of a ligand-nuclease hybrid and their use

Country Status (7)

Country Link
EP (1) EP0589958A4 (de)
JP (1) JPH06510901A (de)
AU (1) AU1972392A (de)
CA (1) CA2108134A1 (de)
IL (1) IL101837A0 (de)
NZ (1) NZ242694A (de)
WO (1) WO1992022642A1 (de)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5436150A (en) * 1992-04-03 1995-07-25 The Johns Hopkins University Functional domains in flavobacterium okeanokoities (foki) restriction endonuclease
EP0682699B1 (de) * 1993-02-12 2003-05-07 The Johns-Hopkins University FUNKTIONELLE DOMÄNEN DER RESTRIKTIONSENDONUKLEASEN AUS -i(FLAVOBAKTERIUM OKEANOKOITES)(FOKI)
CN111751540B (zh) * 2020-07-20 2023-03-03 烟台大学 一种用于核酸酶检测的sers横向流动试纸条及检测方法

Also Published As

Publication number Publication date
CA2108134A1 (en) 1992-12-12
IL101837A0 (en) 1992-12-30
JPH06510901A (ja) 1994-12-08
EP0589958A4 (de) 1995-04-26
AU1972392A (en) 1993-01-12
WO1992022642A1 (en) 1992-12-23
EP0589958A1 (de) 1994-04-06

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