NZ238844A - 4,5-diphenyl-3-phenylaminocarbonyl-pyrrole derivatives - Google Patents

Description

New Zealand Paient Spedficaiion for Paient Number £38844 238844 Cornet** specification Fiied: {5)..C,Oi77lOOL.O.TH..(3.1+.{ .COq .O M-O. S J.QLa of Re9U' '.o. Jc-unua, x;o: ~JA r?W '"'A.

A i®-_5JUUW I V \*C CW? 6$ gjpj|iyj£p Specification has been ante-cfated to 19 &1.

Patents Form No. 5 !njtiafs This is a divisional out of application number 228050 dated 20 February 1989.

NEW ZEALAND PATENTS ACT 1953 COMPLETE SPECIFICATION AN INTERMEDIATE USEFUL IN A PROCESS FOR PREPARING TRANS-6-[2-(SUBSTITUTED-PYRROL -1-YL)ALKYL]PYRAN-2-ONE INHIBITORS OF CHOLESTEROL SYNTHESIS WE, WARNER-LAMBERT COMPANY, A Delaware Corporation, USA, of 201 Tabor Road, Morris Plains, New Jersey 07950, United States of America, hereby declare the invention, for which we pray that a patent may be granted to us, and the method by which it is to be performed, to be particularly described in and by the following statement: (followed by page la) 238844 This invention relates to intermediates that are useful in a process for the preparation of Trans-6-f2-(substituted-pyrrol-l-yl)alkyl]pyran-2-one inhibitors of cholesterol synthesis.

New Zealand patent specification 213252, which is herein incorporated by reference, discloses certain trans-6-[2-(substituted-pyrrol-l-yl)alkyl]-pyran-2-ones. 10 New Zealand patent specification 220409, which is herein incorporated by reference, discloses certain trans-6-[2-(3- or 4-carboxamido-substituted pyrrol-l-yl)alkyl]- 4-hydroxy-pyran-2-ones.

The compounds disclosed in the above New Zealand patent 15 specifications are useful as inhibitors of the enzyme 3-hydroxy-3-methylglutaryl-coenzyme A reductase (HMG-CoA reductase) and are thus useful hypolipidemic and hypocholesterolemic agents. Particularly valuable as hypolipidemic and hypocholesterolemic agents are 20 trans(±) 5-(4-fluorophenyl)-2-(l-methylethyl)-N,4-diphenyl-1- [2- (tetrahydro-4-hydroxy-6-oxo-2H-pyran-2-yl)ethyl]-lH-pyrrole-3-carboxamide and (2R-trans )- - (4-f luorophenyl) -2- (1-methylethyl) -N,4-diphenyl- 1- [2- (tetrahydro-4-hydroxy-6-oxo-2H-pyr an-2 -yl) ethyl ] -25 lH-pyrrole-3-carboxamide. The aforementioned compounds have been prepared by a linear synthetic route which employed two reactions conducted at low temperatures (-78°C) under carefully controlled conditions. The two reactions included the addition 30 of the dianion of ethyl acetoacetate to an aldehyde and the reduction of the hydroxy ketone produced in this reaction with sodium borohydride and a trialkylborane. Although these reactions provide the target compounds in high diastereomeric excess, they (followed by page 2) 2 3 8 8 4 4 Q D 20 are difficult to conduct on large-scale and use expensive reagents which are difficult to handle. They also do not produce enantiomerically pure products. The materials produced by the earlier methods can be separated into enantiomerically pure products but the process is very expensive, time-consuming, and results in the loss of more than 50% of the starting material.

Further,, we have unexpectedly found that the particularly valuable hypolipidemic and hypocholesterolemic agents trans(±)5-(4-fluorophenyl)-2-(l-methyl-ethyl) -N, 4-diphenyl-l- [2- (tetrahydro-4-hydroxy-6-oxo-2H-pyran-2-yl)ethyl] -lH-pyrrole-3-carboxamide and (2 R-trans) -5- (4-fluorophenyl) -2- (1-methylethyl )-N, 4-dipheny 1 -1 - [ 2 - (tetrahydro -4 -hydroxy- 6-oxo-2H-pyran-2-yl) ethyl ]-lH-pyrrole-3-carboxamide can be prepared from a novel intermediate in fewer steps and higher yields than the previous methods. Moreover, the present method proceeds from inexpensive starting materials and is amenable to large-scale synthesis.

This invention provides a compound of Formula XVI XVI 238844 O ° wherein R^q and R^ are independently alkyl of one to eight carbon atons or R10 and R11 together are -CH2-CH- or -CH2-CH2-CH2-• CH3 Preferably, R1Q and R11 are methyl or ethyl or R1Q and R^ together are -CH2CH-. i CH3 The compounds of the formula XVI are useful in a 238 8 process for the preparation of the compound of Formula I, a OH (CH2)2 — " '0' "0 H ■CH(CH3)2 and the hydroxy acid and pharmaceutical^ acceptable salts thereof, corresponding to the opened lactone ring of the compound of Formula I, which comprises a (a) reacting 4-methyl-3-oxo-N-phenylpentanamide with benzaldehyde in the presence of a catalyst and an inert solvent to afford the compounds of Formula XVIII % Ji C = C-C-CH(CH3)2 c=o I NH ~Q XVIII 2 3 8 8 4 (b) reacting the compounds of Formula XVIII with 4-fluorobenzaldehyde in th^ presence of a catalyst, a base, and an inert solvent to afford the compound of Formula XVII XVII (c) reacting the compound of Formula XVII with a compound of Formula ^.ORio NH2CH2CH2-CH SORu wherein R10 and Ru are alkyl of one to eight carbon atoms or R10 and Rlx together are -CH2-CH-, -CH2-CH2- CH3 or -CH2 -CH2 -CH2 - in the presence of a catalyst and an inert solvent to afford a compound of Formula XVI XVI 23 wherein R10 and Ru are alkyl of one to eight carbon atoms or R10 and Ru together are -CH2-CH-, -CH2CH2- CH3 or -ch2-ch2-ch2-; (d) and finally converting a compound of Formula XVI in a conventional manner to afford a compound of Formula I , (e) and if desired, converting the resulting compound of Formula I to a hydroxy acid corresponding to the opened lactone ring of structural Formula I by conventional hydrolysis and further, if desired, converting the hydroxy acid to a corresponding pharmaceutically acceptable salt by conventional means, and if so desired, converting the hydroxy acid to a compound of Formula I by dissolution and/or CI heating in an inert solvent with concomitant removal of water.

In this invention, the term "alkyl" means a straight or branched hydrocarbon group having from one to eight carbon atoms and includes, for example, methyl, ethyl, n-propyl, isopropyl, n-butyl, isobutyl, tertiary-butyl, n-pentyl, tertiary-amyl, n-hexyl, n-heptyl, n-octyl, and the like.

"Cycloalkyl" refers to a three- to six-membered saturated hydrocarbon ring and includes, for example, cyclobutyl, cyclopentyl, cyclohexyl, and the like.

"Alkoxy" is O-alkyl in which alkyl is as defined above. 2388 44 -7- • "Alkanoyloxy" is an alkyl group, as defined above, attached to a carbonyl group and thence, through an oxygen atom, to the parent molecular residue.

"Carboalkoxy" is an alkyl group, as defined above, attached to an oxygen atom and thence, through a carbonyl group, to the parent molecular residue.

"Norbornenyl" is a group derived by the removal of a hydrogen atom (other than at a bridgehead carbon atom) from bicyclo[2.2.1]hept-2-ene.

"Halogen" is iodine, bromine, and chlorine.

"Alkali metal" is a metal in Group XA of the periodic table and includes, for example, lithium, sodium, potassium, and the like.

"Alkaline-earth metal" is a metal in Group IIA of the periodic table and includes, for example, calcium, barium, strontium, and the like.

"Noble metal" is platinum, palladium, rhodium, ruthenium, and the like. > 238844 The compounds of Formula I are useful as inhibitors of the a enzyme 3-hydroxy-3-methylglutaryl-coenzyme A reductase (HMG CoA reductase) and are thus useful as hypolipidemic or hypocholesterolemic agents.

The process of the present invention in its first aspect is an economical, and commercially feasible method for preparing HMG CoA reductase inhibitors of Formula I . d The compound of the Formula I falls within the definition of compounds of the formula I (as defined in New Zealand Patent Specification 228050), that are generally prepared by a process outlined in Scheme I. The compounds of the formula I and methods for preparing them are described and claimed in New Zealand patent specification 228050.

OH I i, r-. - , . /j •. . . _ . . , •■■r~> -■ •-■«- . ......... 238844 SCHEME I oh h2c=chch2-ch—ch2ch=ch2 xi 0 oh ^-V*ch2c-ch2ch=ch2 viii h h ix nc-h2c vti nc - h2c —ch2c02h I I h h vi h2nch2ch2 CH2C02R9a (R9b) h h nc-h2c ch2c02r9a(r9b) h iva(r9a) ivb(r9b) V.CRg.) Vb(^9b! o 238844 o SCHEME I (continued) O » 0 nc-ch2-4^jir- ch2co2h • I h h vi X ch3 r,Xrs ^"2-m-ch2co2-|-ch3 mck2xj-cn2cva h h ch3 : . h h xxv XXI ^•7 ch, h2nch2ch2 —lj-ch2co~c-ch: • I I h h xxnr 3 ch, 238 8 -li- A compound of Formula IX is prepared from the known 1,6-heptadien-4-ol (XI) using the methodology described by Bongini, A., et al, Journal of Organic Chemistry, 47, pp 4626-4633 (1982) and Majewski, M., et al, Tetrahedron Letters, 25, pp 2101-2104 (1984). Thus, the homoallylic alcohol (XI) is reacted with an alkyl lithium such as, for example, n-butyllithium followed by iodine and carbon dioxide to give the iodocarbonate (X) at -35°C to -20°C which is not isolated but treated in situ with a base such as an alkali or alkaline-earth metal hydroxide or carbonate, for example, sodium hydroxide, potassium hydroxide, calcium hydroxide, sodium carbonate, potassium carbonate, calcium carbonate, and the like, in an aqueous alcohol of from one to three carbon atoms such as, for example, methanol, ethanol, isopropanol, and the like, at about 0°C to about 40°C, to afford the epoxide of Formula IX. Preferably the reaction is carried out with potassium carbonate in aqueous methanol at about 0°C to about 40°C, preferably 0°C. The epoxide ring of Formula IX is opened with either potassium or sodium cyanide in an aqueous alcohol such as, for example, methanol, ethanol, tertiary butanol, isopropanol, and the like, at about 0°C to about 40°C. Preferably the reaction is carried out with potassium cyanide in aqueous isopropanol at about 25°C. The resulting diol intermediate is not isolated but treated in situ with a ketal forming reagent such as, for example, acetone, dimethoxypropane, 2-methoxy-propene, benzaldehyde, cyclopentane, cyclohexanone, 1,1-dimethoxycyclopentane, 1,1-dimethoxycyclohexane, and the like, in the presence of an acid such as, for example, camphorsulfonic acid, para-toluenesulfonic acid, and the like, in the presence of excess reagent or in an inert solvent such as, for example, dichloromethane, and the like, at about 0°C to the 2 3 8 8 4 4 reflux temperature of the reagent or solvent to afford a compound of Formula VIII, wherein R7 and Rs are independently hydrogen, alkyl of from one to three carbon atoms, phenyl or R7 and R8 are taken together as -(CH2)n-, wherein n is 4 or 5. A compound of Formula VIII is treated with ozone in an inert solvent such as, for example, dichloromethane and the like, and the resulting intermediate ozonide which is not isolated is flushed in situ with oxygen to remove the ozone and then treated with triphenylphosphine or dimethyl sulfide at about -20°C to about -78°C, preferably about -78°C, to afford a compound of Formula VII, wherein R7 and R8 are as defined above. A compound of Formula VII is treated with an oxidizing reagent such as, for example, chromium trioxide-sulfuric acid-water, and the like, at about 0°C to afford a compound of Formula VI, wherein R7 and R8 are as defined above. A compound of Formula VI is treated with a compound of Formula Hal-Rg a wherein Hal is halogen such as, for example, iodine, chlorine, bromine, and R9_ is alkyl of from one to CI eight carbon atoms, or a three- to six-membered cycloalkyl group, preferably isopropyl, isobutyl, and', the like in the presence of a base such as, for example, l,8-diazabicyclo[5.4.0]undec-7-ene (DBU), and the like to afford a compound of Formula Va, wherein R7, Rg, and Rg are as defined above. Additionally, CL treating the compound of Formula VI with a compound of Formula HO-R9b wherein Rg^ is tertiary butyl, tertiary amyl, or a,a-dimethylbenzyl in the presence of an activating agent such as, for example, dicyclohexylcarbodiimide, 23 8 8 4 4 1, l'-carbonyldiimidazole and the like in the presence of a base such as, for example, 4-dimethylaminopyridine and the like in an inert solvent such as, for example, dichloromethane, tetrahydrofuran, and the like to afford a compound of Formula V^, wherein R7, Rg, and R9b are as defined above. A compound of Formula is treated with hydrogen in the presence of a catalyst such as a noble metal, for example, platinum, palladium, rhodium, ruthenium, derivatives thereof, and the like, or Raney nickel, preferably platinum dioxide, and an acid, such as, for example, acetic acid, propanoic acid and the like, preferably acetic acid, at about 0°c to about 70°C and about 14 to about 100 pounds per square inch pressure to afford a compound of Formula IV. wherein R7, R8/ and Ra are 3 a as defined above. Additionally, a compound of Formula V^ is treated with hydrogen in the presence of a catalyst such as a noble metal, for example, platinum, palladium, rhodium, ruthenium, derivatives thereof, and the like, and an acid such as, for example, acetic acid, propanoic acid and the like, or a catalyst such as, for example, Raney nickel, Raney cobalt and the like, in an inert solvent such as, for example, methanol, ethanol, isopropanol, tetrahydro-furan and the like, saturated with anhydrous ammonia or saturated with aqueous ammonium hydroxide or aqueous sodium hydroxide, preferably the reaction is carried out with Raney nickel in methanol saturated with anhydrous ammonia at about 0°C to about 70°C and about 14 to about 100 pounds per square inch pressure to afford a compound of Formula IV^, wherein R7, R8, and R9b are as defined above. Raney nickel and Raney cobalt as described above are finely divided forms of nickel and cobalt. 238844 A compound of Formula XXI is prepared by treating a compound of Formula VI wherein R7 and Rs are as defined above with hydrogen in the presence of a catalyst such as, for example, Raney nickel, Raney cobalt and the like in finely divided form, in an inert solvent such as, for example, methanol, ethanol, isopropanol, tetrahydrofuran and the like, saturated with anhydrous ammonia or saturated with aqueous ammonium hydroxide solution or a catalyst such as, for example, platinum, palladium and the like, in an inert solvent such as, for example, methanol, ethanol, isopropanol, tetrahydrofuran and the like in the presence of an acid such as, for example, acetic acid, propanoic acid and the like, at about 0°C to about 70°C and about 14 to about 100 pounds per square inch pressure to afford a compound of Formula XXI.

A compound of Formula XXIII is prepared from a compound of Formula XXV h h VI XXV wherein R7 and Rs are as defined above using the , methodology previously described for preparing a compound of Formula XXI from a compound of Formula VI.

A compound of Formula XXV is prepared by treating a compound of Formula VI u o ijk ► • I ncch2 • . ch2co2h • h h vi wherein R7 and Rs are as defined above and tertiary butyl alcohol with a coupling reagent such as, for example, dicyclohexylcarbodiimide and the like in the presence of a base such as, for example, 4-dimethyl-aminopyridine and the like in an inert solvent such as, for example dichloromethane and the like to afford a compound of Formula XXV.

An optically active (R) compound of Formula XXIII, cL is prepared as outlined in Scheme II. The starting material (R)-4-cyano-3-[[(1,1-dimethylethyl)dimethyl-si lyl]oxy]butanoic acid of Formula XXIX is synthesized starting from isoascorbic acid using syntheses well known to practitioners of the art. This chemistry is identical to that disclosed in United States Patent 4,611,067 (Merck & Co. Inc.) using ascorbic acid which is herein incorporated by reference which produces (S)-4-cyano-3-[[(1,1-dimethylethyl)dimethyl-silyl]oxy]butanoic acid.1 1u.s. Patent 4,611,067 apparently incorrectly assigned the configuration R to the product of this sequence of reactions starting with ascorbic acid. ^24 OCT 1991*1 o 2 3 8 8 4 4 SCHEME II "J ch3 ch3—c-ch3 CH3 —Si — CH3 0 T n=c - ch2chch2co2h xxix ch3 CH3-c-CH3 ch3—si—ch3 0 T ch, nscch2chch2c0ch2c02-c —ch3 xxvir1 ch3 h 0 f ch3 N s cch2chch2 coch2co2 - c - ch3 xxvii ch3 H 0 T h o T ch3 ns cch2chch2chch2co2—c—ch3 xxvi ch3 X 0 o 1 » nsscchjcv^c-chjicoj — c-ch3 ch3 x o 0 ■ « h2nch2ch2c s,^. c - ch2co2 — c - ch3 ch3 H h ch3 h h I ch3 XXVa xxiila • ♦ * / o 23884 Thus, the optically active compounds are prepared from the known isoascorbic acid using the methodology described by Volante R. P. et al, in United States r\ Patent 4,611,067 but in that case starting with 5 ascorbic acid. This establishes the optically active centers desired in Formula XXV a and Formula XXIII as R. Thus, the (R)-4-cyano-3-[[(1,1-dimethylethyl)-dimethylsilyl]oxy]butanoic acid of Formula XXIX is treated with carbonyldiimidazole in tetrahydrofur an at 10 0°C to -40°C, preferably -20°C, warmed to 25°C and the activated acid derivative is not isolated but the solution is added to a suspension of a salt of 1,1-dimethylethyl malonic acid such as, for example, the potassium salt of 1,1-dimethylethyl malonic acid 15 (half ester, half salt) anhydrous magnesium chloride, and an amine such as, for example, diisopropylethylamine in acetonitrile at -10°C to 20°C preferably at 5°C. The mixture is poured into a mixture of IN hydrochloric acid and ethyl acetate to afford the (R)-l,1-dimethyl-20 ethyl 6-cyano-5-[(1,1-dimethylethyl)dimethylsilyl]oxy-3-oxohexanoate of Formula XXVIII. The ketone of Formula XXVIII is treated with fluoride ion at 0°C to ^ 65°C, preferably 25°C to afford the (R)-l, 1-dimethyl ethyl 6-cyano-5-hydroxy-3-oxo-hexanoate of 25 Formula XXVII. The ketone of Formula XXVII is treated with triethylborane and air (or methoxydiethylborane . _ , without air) followed by sodium borohydride and methanol in tetrahydrofuran at -78°C to -110°C, preferably -100°C to afford [R-(R*,R*)]-l,l-dimethyl-30 ethyl 6-cyano-3,5-hydroxyhexanoate of Formula XXVI. The diol of Formula XXVI is treated with a ketal forming reagent such as, for example, acetone, dimethoxypropane, 2-methoxypropene, benzaldehyde, cyclopentanone, cyclohexanone, 1,1-dimethoxycyclo-35 pentane, 1,1-dimethoxycyclohexane and the like, in the presence of an acid such as, for example, camphorsulfonic acid, para-toluenesulfonic acid, and the like, in the presence of excess reagent or in an inert solvent such as, for example, dichloromethane, and the like, at 0°C to the reflux temperature of the reagent or solvent to afford a compound of Formula XXVa wherein R7 and R8 are independently hydrogen, alkyl of from one to three carbon atoms, phenyl or R7 and R8 are taken together as -(CH2)n-, wherein n is 4 or 5.

A compound of Formula XXVa is treated with hydrogen gas in an alcohol such as methanol saturated with anhydrous ammonia or aqueous ammonium hydroxide in the presence of a catalyst such as Raney nickel or Raney cobalt or with a nobel metal catalyst such as platinum oxide in the presence of an alkanoic acid such as acetic acid to afford a compound of Formula XXIII . wherein R7 and R8 as defined above. a Additionally, an optically active compound of Formula IV or Formula IV^ may be prepared starting from the optically active epoxide of Formula IX. The preparation of the optically active epoxide of Formula IX is described by Kocienski, P. J., et al, Journal of the Chemical Society Perkin Transaction I, pp 2183-2187 (1987).

The process of preparing a compound of Formula I is outlined in Scheme III: 238844 SCHEME III R.7 Ro oXo H2NCH2CH2 —CH2C02R9a 1 i H .H IVa (R9a) IVb (R9b) 0 0 II II (R9b) Rj. ~~ C — CH CH C R2 R3 III R?v yR® Ri X 0 0 N-CH2CH2 CH2C02R9a (^9b) 1 I H H R4 na(R9«) irb(R9b) OH ,<1_l , " r J,? , gl -«!^-1'fM ''•[ 1- *I"/^X •» _ . 23 o A compound of Formula iva or Formula IV^ is reacted with a compound of Formula III, ^ wherein Rx is 1-naphthyl, 2-naphthyl, cyclohexyl, cyclohexylmethyl, norbornenyl, phenyl, phenyl substituted with fluorine, chlorine, bromine, hydroxyl, trifluoromethyl, alkyl of from one to four carbon atoms, alkoxy of from one to four carbon atoms, or alkanoyloxy of from two to eight carbon atoms, benzyl, 2-, 3-, or 4-pyridinyl, or 2-, 3-, or 4-pyridinyl-N-oxide; R2 or R3 is independently hydrogen alkyl of from one to six carbon atoms, cyclopropyl, * * cyclobutyl, cyclopentyl, cyclohexyl, phenyl, phenyl substituted with fluorine, chlorine, bromine, hydroxyl, 23 8 8 4 4 trifluoromethyl, alkyl of from one to four carbon atoms, or alkoxy of from one to four carbon atoms, cyano, trifluoromethyl, or -CONRsR6 where R5 and R6 are independently hydrogen, alkyl of from one to six carbon atoms, phenyl, phenyl substituted with fluorine, chlorine, bromine, cyano, or trifluoromethyl; R4 is alkyl of from one to six carbon atoms, cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, or trifluoromethyl; in an inert solvent such as, for example, toluene, and the like, at the reflux temperature of the solvent to give a compound of Formula IIa, or Formula 11^, wherein Rx, R2, R3, R4, R7, Rs, R9a and Rg^ are as defined above. Finally, a compound of Formula II, is cL treated with an acid such as, for example, aqueous hydrochloric acid, and the like, in an inert solvent such as, for example, tetrahydrofuran, followed by hydrolysis with a base such as, for example sodium hydroxide. The reaction is neutralized with an acid such as, for example, aqueous hydrochloric acid and dissolved and/or heated in an inert solvent such as, for example, toluene, and the like, with concomitant 2 3 8 8 4 4 removal of water to give a compound of Formula I, wherein Rx, R2, R3, and R4 are as defined above.

Additionally, a compound of Formula 11^ is treated with an acid such as, for example, aqueous 5 hydrochloric acid, and the like, in an inert solvent such as, for example, tetrahydrofuran and the like for about 15 hours, followed by the addition of a base such as, for example, sodium hydroxide and the like and stirred for about 30 hours. The reaction is 10 reacidified with an acid such as, for example, aqueous hydrochloric acid and dissolved and/or heated in an inert solvent such as, for example, toluene and the like, with concomitant removal of water to give a compound of Formula I, wherein Rx, R2, R3, and R4 are 15 as defined above.

A compound of Formula I can also be prepared by a process as outlined in Scheme IV: 238844 SCHEME IV r7 r9 n^n 0 0 nh2-ch2ch2'^s,>^s ch2co2h xxi R7 Rg n^n 0 0 JL X 'H3 nh2—ch2ch2"""—. ch2co2 — c - ch3 ch3 xxiii xvii R7 Ra 0 0 xxii *7x*0 0 o (ch2) 2 ch2co2 - c - ch3 n h h i " ch(ch3)2 ch3 ch3 v-ch (ch xxiv 23884 The compound of Formula XVII is reacted with a compound of Formula XXI nh2-ch2ch2 xxi wherein R7 and R8 are independently hydrogen, alkyl of from one to three carbon atoms, phenyl or R7 and R8 are taken together as -(CH2)n~ wherein n is 4 or 5 in the presence of an inert solvent such as, for example, dimethyl sulfoxide and the like for about 15 hours at about 105°C with the removal of water to afford the intermediate derivative (XXII). Preferably the reaction is carried out in dimethyl sulfoxide for about 15 hours at about 105°C. The intermediate derivative XXII, which is not isolated, is treated with an acid such as, for example, concentrated hydrochloric acid and the like in an inert solvent such as, for example, ethyl acetate and the like to afford the compound of Formula I . Preferably, the CI reaction is carried out with concentrated hydrochloric acid in ethyl acetate. 1• \ 2 4 OCT 1991^ Additionally, the compound of Formula XVII is reacted with a compound of Formula XXIII R7 Rg h2nch2ch2 j h I • ?s 'H3 « ch2co2—c-ca3 H H CH3 XXIII wherein R7 and Rs are independently hydrogen, alkyl or from one to three carbon atoms, phenyl or R7 and R8 are taken together as -(CH2) -, wherein n is 4 or 5 in the presence of an inert solvent or solvents such as, for example, hexane, toluene and the like for about 24 hours at about the reflux temperature of the solvent or solvents. The intermediate derivative XXIV, which is not isolated, is treated with an acid such as, for example, a 10% aqueous solution of hydrochloric acid for about 15 hours, followed by the addition of a base such as, for example, sodium hydroxide and the like and reacidification with an acid for about 30 hours to afford the compound of Formula I . d The process for preparing the HMG CoA reductase inhibitor of Formula I is outlined in Scheme V: a i 24 OCT 19 u 0 CH2-C-CH(CH3)2- c=0 OCHj XX CH2-C-CHlCH3h-c=0 NHi ""O XIX SCHEME V 0 H II _ ^ "c=C-C"CH (Cllj) 2 <6 »»°-Q win ^ "-0 XVII I 0H « « F (CH2) 2-CHCH2-CCH2-C-OCH3 CH (CH3); CH2CH2CHO -CH(CH3); ^ORio ch2ch2-ch-^orii CH (CHj); I OH 2H o (CH2)2-CHCH2-CHCH2-C-OCH3 f OH °H ich2)2-chch2-chch2-co2h OH c? tr^ CH(CH3); CH(CH3)2 11 i - S° '0 11 H 1 N -CH (CH3) 2 XII o 23 8 8 D 4-Methyl-3-oxo-N-phenylpentanamide (XIX) is obtained by heating a mixture of methyl 4-methyl-3-oxopentanoate (XX), aniline and ethylene diamine in toluene. 4-Methyl-3-oxo-N-phenylpentanamide (XIX) is 5 subsequently reacted with benzaldehyde in the presence of a catalyst such as, for example, piperidine and glacial acetic acid, ethylene diamine and glacial acetic acid, p-alanine and glacial acetic acid, and the like in an inert solvent such as, for example, 10 toluene, heptane, hexane, and the like for about 24 to about 36 hours at about 60° to about 120°C with the removal of water to afford 4-methyl-3-oxo-N-phenyl-2-(phenylmethylene)pentanamide (XVIII). Preferably the reaction is carried out with p-alanine and glacial 15 acetic acid at reflux for about 24 hours in hexane. The 4-methyl-3-oxo-N-phenyl-2-(phenylmethylene)-pentanamide (XVIII) is reacted with 4-fluorobenz-aldehyde in the presence of a catalyst such as, for example, 3-benzyl-5-(2-hydroxyethyl)-4-methylthiazolium 20 chloride, 3,4-dimethyl-5-(2-hydroxyethyl)thiazolium iodide, 3-ethyl-5- (2-hydroxyethyl) -4-methylthiazolium bromide, thiamine hydrochloride, and the like, and a base such as, for example, N,N-diisopropylethylamine, pyridine, N,N-dimethy1aniline, triethylamine, 25 l,8-diazabicyclo[5.4.0]undec-7-ene (DBU), 1,4-diaza-bicyclo[2.2.2]octane (DABCO), 4-dimethylaminopyridine (DMAP), N,N,N',N'-tetramethylethylenediamine (TMEDA) and the like, either neat or in the presence of a solvent such as, for example, tetrahydrofuran, 30 tertiary-butyl methyl ether, ethanol, dimethyl- formamide, dimethylsulfoxide, N-methylpyrrolidone, acetonitrile, methylisobutyl ketone, ethyl acetate, isopropanol, pyridine and the like for about 20 to about 30 hours under anhydrous conditions at about 35 room temperature to about the reflux temperature of the solvent to afford the compound of Formula XVII. 238844 o -28-' Preferably the reaction is carried ouu in the presence of 3-ethyl-5-(2-hydroxye"chyl)-4-methylthiazolium bromide and triethylamine in ethanol at about 75-30°C for about 24 hours. The compound of Formula XVII is 5 reacted with a compound of Formula ^ ORj.0 NK2CH2CH2-CH S0RU wherein RL0 and &n are alkyl of one to eight carbon 10 atoms or Rl0 and Ru together are -CH2-CE-, -CH2CH2- CH3 or -CH2CH2CH2- in the presence of a catalyst of Formula Ri, 2 C02H wherein Rj,2 is CH3 / CF3 , ClCH2*~, CgHgCH2CH2 — , CgrijCHo —, H02CCH2-, H02CCH2CH2-, CsE5-, Dara-Cl-C6H5-, ClCE2CH2-, meta-H3C-CsH5-, oara-H3C-CsH5-, tertiary-CA5g- or triethylamine hydrochloride and a solvent or mixtures thereof such as, for example, tetrahydrofuran, hexane, 20 toluene, ethanol, tertiarv-buty1 acetate, ethyl acetate, 1,2-dichloroethane, isopropanol, dimethyl sulfoxide and the like for about 24 to about 48 hours - - ' at about 5°C to about the reflux tempera-cure of the solvent with the removal of water to afford a compound 25 of Formula XVI. Preferably, the reaction is carried out in the presence of pivalic acid and a mixture of toluene and heptane at reflux for abou~ 43 hours with the removal of water. A compound of Formula XVI is converted in a conventional manner using the 30 methodology disclosed in New Zealand patent specification 220409 to the compound of Formula I . r.

C2A OCT 1991 238844 Certain of the compounds of Formula III are either known or capable of being prepared by methods known in the art. The ring-opened dihydroxy acids of formula HO .H C02H n-ch2ch2- H wherein ri, r2, r3, and r4 are as defined above may be prepared from the lactone compounds of Formula I by conventional hydrolysis such as, for example, sodium hydroxide in methanol, sodium hydroxide in tetrahydro-furan-water, and the like, of the lactone compounds of Formula I.

The ring-opened dihydroxy acid of Formula XII may be produced from the lactone compound of Formula I, by cL conventional hydrolysis of the lactone compound of Formula I. ci In the ring-opened dihydroxy acid form, the compounds of Formula I react to form salts with pharmaceutically acceptable metal and amine cations formed from organic and inorganic bases. The term "pharmaceutically acceptable metal salt" contemplates salts formed with the sodium, potassium, calcium, magnesium, aluminum, iron, and zinc ions. The term "pharmaceutically acceptable amine salt" contemplates salts with ammonia and organic nitrogenous bases strong enough to form salts with carboxylic acids.

Bases useful for the formation of pharmaceutically acceptable nontoxic base addition salts of the compound of the present invention form a class whose. 23 8 8 4 4 limits are readily understood by those skilled in the art.

The dihydroxy free acid form of the compounds of Formula I may be regenerated from the salt form, if desired, by contacting the salt with a dilute aqueous solution of an acid such as hydrochloric acid.

The ring closed lactone form of the compounds of Formula I may be regenerated by dissolution of the dihydroxy acid form of the compounds of Formula I in an inert solvent such as, for example, toluene, benzene, ethyl acetate, and the like, at about 0°C to about the boiling point of the solvent usually but not necessarily with concomitant removal of the resulting water and usually but not necessarily with strong acid catalysis such as, for example, concentrated hydrochloric acid and the like.

The base addition salts may differ from the free acid forms of the compounds in such physical characteristics as solubility and melting point, but are otherwise considered equivalent to the free acid form.

The compounds may exist in solvated or unsolvated form and such forms,.are equivalent to the unsolvated form for the purposes of this invention.

The compounds of structural Formulas I, I=, and a XII above possesses two asymmetric carbon centers, one at the 4-hydroxy position of the pyran-2-one ring, and the other at the 6-position of the pyran-2-one ring where the alkylpyrrole group is attached. This asymmetry gives rise to four possible isomers, two of which are the 4R,6S and 4S, 6R-isomers and the other two of which are the 4R,6R and 4S, 6S-isomers. The preferred isomer is the 4R,6R-isomer of the compounds of Formulas I, I and XII above.

CL X o 23 8 8 4 O The following nonlimiting examples illustrates the inventors1 preferred method for preparing the compounds of Formula I.

EXAMPLE 1 Trass-6-f 2-f 2-ethyl-5-(4-fluorophenyl)-lH-pyrrol-l-yl1 -ethyl 1 -tetrahydro-4-hydroxy-2H-pyran-2-one.

Step A: Preparation of (B*,£*)-a-2-propenyloxirane-ethanol. n-Butyllithium, 129 mL (200 mmol), is added 10 dropwise to a 0°C solution of 1,6-heptadien-4-ol, 22.4 g (0.2 mol), in 200 mL of anhydrous tetrahydro-furan until the triphenylmethane indicator turned red. Carbon dioxide is then bubbled in for 30 minutes (lecture bottle carbon dioxide passed through 15 drierite) and the light yellow solution is stirred for 30 minutes under a balloon of carbon dioxide. To this solution is added iodine, 101.4 g (0.4 mol), dissolved in ^200 mL of anhydrous tetrahydrofuran over 60 minutes. The mixture is allowed to warm to room temperature overnight, diluted with ethyl acetate, washed with 10% sodium bisulfite solution, saturated solution of sodium bicarbonate, brine, and dried (magnesium sulfate). The crude product is dissolved in 200 mL of methanol and 20 mL of water, cooled to The mixture is vigorously stirred for six hours, filtered, concentrated, and partitioned between ethyl acetate and brine. After extracting the aqueous layer 2x with ethyl acetate, the combined organics are 30 washed with brine and dried (magnesium sulfate).

Flash chromatography (4:1 hexane-ethyl acetate) provides, after concentration in vacuo, 18 g of (R*,R*)-a-2-propenyloxiraneethanol.

O 0°C and 0.5 g of solid potassium carbonate is added. 200 MHz NMR (CDC13) 6 1.5-1.65 (m, 1H), 1.90 (dt, 1H, J = 15, 4 Hz), 2.2 (m, 3H), 2.53 (m, 1H), 2.79 (m, 1H), 3.12 (m, 1H), 3.94 (m, 1H), 5.19 (m, 2H), 5.80 (m, 1H).

IR (film) 3400, 3077, 2980, 2925, 1643, 1412, 1260, 918, 827 cm-1.

Step B: Preparation of (±)-cig-2,2-dimethyl-6-(2-propenyl)-1,3-dioxane-4-acetonitrile.

Potassium cyanide, 1.3 g (20 mmol), is added to a room temperature solution of (R*,R*)-ot-2-propenyl-oxiraneethanol, 2.56 g (20 mmol), in 25 mL of 4:1 isopropanol-water. The solution is stirred for 20 hours at ambient temperature, concentrated, and partitioned between ethyl acetate and brine. The aqueous layer is extracted 2x with ethyl acetate and the combined ethyl acetate extracts are washed with brine and dried (magnesium sulfate). The crude product is dissolved in 20 mL of 2,2-dimethoxypropane, camphorsulfonic acid is added and the solution stirred for 18 hours at room temperature. Concentration and flash chromatography provides 1.30 g of (±)-cis-2,2-dimethyl-6-(2-propenyl)-1,3-dioxane-4-acetonitrile. 200 MHz NMR (CDC13) '6 1.35 (m, 1H), 1.40 (s, 3H), 1.45 (s, 3H), 1.67 (m, 1H), 2.20 (m, 1H), 2.33 (m, 1H), 2.50 (m, 2H), 3.89 (m, 1H), 4.10 (m, 1H), 5.10 (m, 2H), 5.7-5.9 (m, 1H).

IR (film) 2995, 2944, 2255, 1644, 1334 cm"1.

Step C: Preparation of (±)-cis-6-(2-oxoethyl)-2,2-dimethyl-1,3-dioxane-4-acetonitrile.

A solution of (±)-cis-2,2-dimethyl-6-(2-propenyl)-l,3-dioxane-4-acetonitrile, 3 g (15.36 mmol), in 238 100 mL of dichloromethane is cooled to -78°C under nitrogen. Ozone (Welsbach generator, flow rate 0.1, voltage = 90V) is then passed through a fritted gas inlet tube into the solution until the blue color of ozone appears. The current is turned off, and oxygen bubbled through until the blue color is discharged. Triphenylphosphine, 4.2 g (16 mmol), is added and the colorless solution allowed to warm to room temperature. Flash chromatography provides, after concentration in vacuo, 2.5 g of pure (±)-cis-6-(2-oxo ethyl) -2,2-dimethyl-l, 3-dioxane-4-acetonitrile. 200 MHz NMR (CDC13) 6 1.30 (m, 1H), 1.39 (s, 3H), 1.48 (s, 3H), 1.78 (m, 1H), 2.46-2.75 (m, 4H), 4.2 (m, 1H), 4.40 (m, 1H), 9.79 (t, 1H, J = 1.6 Hz).

IR (film) 2250, 1720 cm-1.

Step D: Preparation of (±)-cjs-6-(cyanomethyl)-2,2-dimethyl-l,3-dioxane-4-acetic acid.

Jones reagent (chromium trioxide-sulfuric acid-water), 3.8 mL (7.6 mmol), is added dropwise to a 0°C solution of (±)-cis-6-(2-oxoethyl)-2,2-dimethyl-l,3-dioxane-4-acetonitrile, 1.50 g (7.6 mmol), dissolved in 50 mL of acetone until the orange color is not discharged. After stirring a further 15 minutes, the mixture is poured into 300 mL of diethyl ether and washed with brine until the aqueous washes are colorless. The diethyl ether layer is dried (magnesium sulfate), filtered, and concentrated to provide 1.2 g of the acid which solidifies on standing. Trituration with isopropyl ether provides (±)-cis-6-(cyanomethyl) -2,2-dimethyl-l, 3-dioxane-4-acetic acid as a colorless solid; mp 92-95°C. A second trituration/recrystallization from isopropyl ether provides material of mp 98-103°C. 200 MHz NMR (CDC13) 6 1.35 (m, 1H), 1.42 (s, 3H), 1.49 (s, 3H), 1.82 (m, 1H), 2.4T2.7 (m, 4H), 4.18 (m, 1H), 4.35 (m, 1H). 13C-NMR(d6-acetone, 50 MHz) 6 19.95, 24.91, 30.17, 35.88, 41.34, 65.79, 66.35, 99.70, 117.77, 171.83.

IR (KBr) broad OH 3500-2400, 2254, 1711, 940 cm-1.

Step E: Preparation of (±)-cjs-l-methylethyl 6-(cyanomethyl ) -2 ,2-dimethyl-l,3-dioxane-4-acetate. To a solution of (±)-cis-6-(cyanomethyl)-2,2-dimethyl-l,3-dioxane-4-acetic acid, 0.6 g (3 mmol), in acetonitrile, 2 mL, is added l,8-diazabicyclo[5.4.0]-undec-7-ene (DBU), 0.45 mL (3 mmol), and 2-iodopropane, 0.33 mL (3.3 mmol). The solution is stirred overnight at room temperature, diluted with diethyl ether, washed with brine, and dried (magnesium sulfate).

Flash chromatography provides 0.55 g of (±)-cis-l-methylethyl 6-(cyanomethyl)-2,2-dimethyl-l,3-dioxane-4-acetate. 90 MHz NMR (CDCl3) 6 1.22 (d, 6H, J = 7 Hz), 1.3 (m, 1H), 1.35 (s, 3H), 1.40 (s, 3H), 1.75 (m, 1H), 2.2-2.7 (m, 4H), 3.9-4.4 (m, 2H), 4.95 (septet, 1H, J = 7 Hz).

Step F: Preparation of (±)-cis-l-methylethyl 6-(2- aminoethyl)-2,2-dimethyl-l, 3-dioxane-4-acetate A mixture of (±)-cis-l-methylethyl 6-(cyanomethyl) 2,2-dimethyl-l,3-dioxane-4-acetate, 0.55 g, in glacial acetic acid is hydrogenated with platinum dioxide at 50 pounds per square inch (PSI). Concentration, dilution with ethyl acetate and bicarbonate wash, followed by washing with brine and drying provides 250 mg of (±)-cis-l-methylethyl 6-(2-aminoethyl)-2,2-dimethyl-l,3-dioxane-4-acetate. MS 260.1, 244.1 238844 200 MHz NMR (CDC13) 6 1.25 (d, 6H, J = 7 Hz), 1.32 (m, 1H), 1.36 (s, 3H), 1.45 (s, 3H), 1.60 (m, 1H), 2.33 (dd, 1H, J = 15, 6 Hz), 2.49 (dd, 1H, J = 15, 6 Hz), 2.85 (br t, 2H, J = 6 Hz), 3.40 (br s, 2H), 4.00 (m, 5 1H), 4.29 (m, 1H), 12.03 (septet, 1H, J = 7 Hz).

IR (film) 1734, 1387 cm-1.

Step G: Preparation of (±)-cig-l-methylethyl 6-[2-T 2-ethyl-5-(4-fluorophenyl)-lg-pyrrol-l-yl] ethyl!-2,2-dimethyl-l,3-dioxane-4-acetate. 10 A solution of (±)-cis-l-methylethyl 6-(2-amino- ethyl)-2,2-dimethyl-l,3-dioxane-4-acetate, 0.15 g (0.58 mmol), and l-(4-fluorophenyl)-l,4-hexanedione (Example A), 0.125 g (0.6 mmol), in 5 mL of toluene is stirred and heated at reflux overnight. The cooled 15 solution is concentrated and the highly UV active pyrrole is separated from starting material by preparative thin layer chromatography, eluting 2x with 4:1 hexane-ethyl acetate. This provides 130 mg of pure (±)-cis-l-methylethyl 6-[2-[2-ethyl-5-(4-fluoro-20 phenyl)-lH-pyrrol-l-yl]ethyl]-2,2-dimethyl-l,3-dioxane< 4-acetate. 200 MHz NMR (CDCl3) 6 1.51 (m, 1H), 1.23 (d, 6H, J = 6 Hz), 1.33 (m, 9H), 1.5-1.6 (m, 3H), 2.27 (dd, 1H, - * J = 15.3, 6 Hz), 2.44 (dd, 1H, J = 15.3, 6 Hz), 2.66 25 (q, 2H, J = 7.5 Hz), 3.62 (m, 1H), 3.8-4.2 (m, 3H), .03 (septet, 1H, J = 6 Hz), 5.97 (d, 1H, J = 3.5 Hz), 6.11 (d, 1H, J = 3.5 Hz), 7.0-7.4 (m, 4H).

A solution of (±)-cis-l-methylethyl 6-[2-2-ethyl- -(4-fluorophenyl)-lH-pyrrol-l-yl]ethyl]-2,2-dimethyl-30 l,3-dioxane-4-acetate, 0.13 g (0.3 mmol), in 12 mL of 1:2 2M hydrochloric acid-tetrahydrofuran is stirred overnight at room temperature. To this is added 238 8 4 f* sufficient 2M sodium hydroxide to bring the pH to 10. Stirring is continued for 30 minutes, water, 30 mL, is added and the mixture is extracted with diethyl ether. The aqueous layer is acidified with ice cold 6N hydrochloric acid and extracted with ethyl acetate (2x). The organic layer is washed with brine and dried (magnesium sulfate). The residue which remains on filtration and concentration is dissolved in toluene (50 mL) and heated at reflux with azeotropic removal of water (6 hours). The cooled solution is concentrated and flash chromatographed to provide 60 mg of trans-6-\2-\2-ethyl-5-(4-fluorophenyl)-1H-pyrrol-l-yl]ethyl]-tetrahydro-4-hydroxy-2H-pyran-2-one (elution with 2:1 hexane-ethyl acetate). 90 MHz NMR (CDCL3) 6 1.25 (d, 6H, J = 7 Hz) 1.3-1.8 (m, 4H), 2.3 (br s, 1H, -OH), 2.55 (m, 2H), 2.65 (q, 2H, J = 7Hz), 3.9-4.5 (m, 4H), 5.90 (d, 1H, J = 3.5 Hz), 6.05 (d, 1H, J = 3.5 Hz), 6.9-7.4 (m, 4H).

EXAMPLE 2 Tg£ns-(±)-5-(4-fluorophenyl)-2-(1-methylethyl)-N,4-diphenyl-1-[2-(tetrahydro-4-hydroxy-6-oxo-2g-pyran-2-yl)ethyl1-lg-pyrrole-3-carboxamide.

METHOD A Step A: Preparation of 4-Methyl-3-oxo-N-phenyl-2-(phenylmethylene)pentanamide.

A suspension of 100 kg of 4-methyl-3-oxo-N-phenyl-pentanamide (Example B) in 660 kg of hexanes is treated with agitation under nitrogen with 8 kg of P-alanine, 47 kg of benzaldehyde, and 13 kg of glacial acetic acid. The resulting suspension is heated to reflux with removal of water for 20 hours. An 2 3 8 8 4 4 additional 396 kg of hexanes and 3 kg of glacial acetic acid is added and reflux continued with water removal for one hour. The reaction mixture is cooled to 20-25°C and the product is isolated by filtration. The product is purified by slurrying in hexanes at 50-60°C, cooling, and filtration. The product is slurried twice with water at 20-25°C, filtered, and dried in vacuo to yield 110 kg of 4-methyl-3-oxo-N-phenyl-2-(phenylmethylene)pentanamide, mp 143.7-154.4°C.

Vapor Phase Chromatography (VPC): 30 meter DB 5 capillary column 50-270°C at 15°C/min. 19.33 min, 99.7% (area).

Gas Chromatography/Mass Spectrometry (GC/MC): M/Z 293 [M]+.

Nuclear Magnetic Resonance (NMR): (CDC13) 6 1.16 (6H, d), 3.30 (1H, quin.)/ 7.09 (1H, m), 7.28 (5H, m), 7.49 (5H, m), 8.01 (1H, brs).

Step B: Preparation of (±)4-Fluoro-ot-|"2-methyl-l- oxopropyl ] -y-oxo-N, p-diphenylbenzenebutane-amide mixture of TR-(R*>R*)1, [R-(R*,S*)], [S-(R*,R*)1 and fS-(R*,S*)1 isomers.

A solution of 17.5 kg of 3-ethyl-5-(2-hydroxy-ethyl)-4-methylthiazolium bromide in 300 L of anhydrous ethanol is concentrated by distillation of 275 L of the ethanol. Under an argon atmosphere, 100 kg (340 mol) of 4-methyl-3-oxo-N-phenyl-2-(phenylmethylene)pentanamide, 47.5 L (340 mol) of triethylamine, and 40 L (375 mol) of 4-fluorobenz-aldehyde are added. The resulting solution is stirred and heated at 75-80°C for 23 hours. The product begins to form as a solid after approximately 2388 1-5 hours but approximately 24 hours is required for essentially complete conversion. The slurry is dissolved in 600 L of isopropanol at 80°C. The resulting solution is slowly cooled and the (±)4-fluoro-a-[2-methyl-l-oxopropyl]-y-oxo-N, p-di-phenylbenzenebutaneamide mixture of [R-(R*,R*)], [ R- (-R*,S*)], [S-(R*, R*) ] and [S-(R*,S*)] isomers isolated by filtration. Washing the precipitate with isopropanol and drying in vacuo yielded 99 kg of (±)4-fluoro-cr-[2-methyl-l-oxopropyl]--y-oxo-N, p-diphenyl benzenebutaneamide mixture of [R-(R*,R*)], [R-(R*,S*)], [S-(R*,R*)] and [S-(R*,S*)] isomers; mp 206.8-207.6°C.

NMR: (CDCls) 6 1.03 (3H, d), 1.22 (3H, d), 2.98 (1H, quin.), 4.91 (1H, d, J = 11 Hz), 5.51 (1H, d, J = 11 Hz), 6.98-7.43 (12H, m), 8.17 (2H, dd), 9.41 (1H, brs).

High Pressure Liquid Chromatography (HPLC): (Acetonitrile:tetrahydrofuran:water) (40:25:55) Econosil Cl85|j 25 cm 1.0 mL/min. 254 nm 16.77 min. 99.2% (area).

Step C: Preparation of l-(3,3-Diethoxypropyl)- - (4-fluorophenyl)-2-(1-methylethyl)-N,4-diphenyl-lH-pyrrole-3-carboxamide. To a nitrogen purged flask equipped with a mechanical stirrer is added 130 kg (311 mol) of (±)4-fluoro-a- [2-methyl-l-oxopropyl ]-y-oxo-N, p-di-phenylbenzenebutaneamide mixture of [R-(R*,R*)], [R-(R*,S*)], [S-(R*,R*)] and [S-(R*,S*)] isomers, 540 L of heptanes and 60 L of toluene, 59 kg (400 mol) of 3-amino-1,1-diethoxypropane, and 22.3 kg (218 mol) of pivalic acid. The mixture is stirred and heated to reflux, removing water with a Dean Stark trap. The 2 3 8 8 4 4 mixture is refluxed 32 hours and slowly cooled to 60-65°C, diluted with 500 L of 2-propanol-water (3:2), seeded, and cooled to 20-25°C. The product is isolated by filtration, washed with 300 L of 2-propanol, and dried in vacuo to yield 133.5 kg of 1-(3,3-diethoxypropyl)-5-(4-fluorophenyl)-2-(l-methyl-ethyl)-N,4-diphenyl-lH-pyrrole-3-carboxamide; mp 125.1-127.7°C after recrystallization from ethanol.

HPLC: (acetonitrile:tetrahydrofuran:water) (40:25:55) 1.5 mL/min 254 nm Econosil C18 5p 25 cm R.T. = 37.70 min 99.4% (area) NMR: ((CD3)2CO) 6 1.04 (6H, m, t), 1.47 (6H, d), 1.82 (2H, m), 3.40 (5H, m), 3.99 (2H, m), 4.43 (1H, brt), 6.90-7.50 (14H, m), 8.26 (1H, brs) In a process analogous to Step C using appropriate starting materials, the following compounds of Formula XVI are prepared: l-(3,3-Dimethoxypropyl)-5-(4-fluorophenyl)-2-(1-methyl-ethyl)-N,4-diphenyl-lH-pyrrole-3-carboxamide; mp 167-168.2°C. -(4-Fluorophenyl)-l-[2-(4-methyl-l,3-dioxolan-2-yl)-ethyl1-2-(1-methylethyl)-N,4-diphenyl-lH-pyrrole-3-carboxamide; bp 141.5-145.9°C.

Step D: Preparation of 5-(4-Fluorophenyl)-2-(l- methylethyl)-1-(3-oxopropvl)-N,4-diphenyl-lH-pyrrole-3-carboxamide.

To a nitrogen purged flask fitted with an overhead stirrer, a thermometer, and a condenser is added 20 kg (37.8 mol) of 1-(3,3-diethoxypropyl)-5-(4-fluorophenyl)-2-(1-methylethyl)-N,4-diphenyl- lH-pyrrole-3-carboxamide along with 200 L of acetone. The solution is stirred and 100 L of 2N hydrochloric acid solution is added. The mixture is heated to reflux for four hours then cooled to 50°C ± 5°C, 5 seeded, and cooled to 0°C ± 5°C. The product is collected by filtration, washed with 100 L 2-propanol-water (1:1) and dried in vacuo at 50°C for 64 hours to yield 16.2 kg of 5-(4-fluorophenyl)-2-(l-methylethyl)-1-(3-oxopropyl)-N,4-diphenyl-lH-pyrrole-3-carboxamide 10 as an off-white solid.

Step E: Preparation of 2-(4-Fluorophenyl)-6-hydroxy-5-(1-methylethyl)-P-oxo-3-phenyl-4-f(phenyl-amino )carbonyl]-lg-pyrrole-l-heptanoic acid, methyl ester.

A 22-L three-necked flask fitted with an overhead stirrer, a low temperature thermometer, and a 2-L calibrated addition funnel is dried with a nitrogen purge and 78 g (1.95 mol) 60% sodium hydride in mineral oil is added, followed by 248 mL (1.76 mol) 20 diisopropylamine and 8 L tetrahydrofuran. The reaction is cooled to -10° to 0°C, a significant nitrogen purge was introduced through the flask, and 212 mL (1.92 mol) methyl acetoacetate is added in a slow stream over a 10 to 30-minute period. Stirring 25 is continued at -10° to 10°C for an additional 10 to - • - 30 minutes. After cooling to -15 to -5°C, 2.2 L of 1.6 M n-butyllithium in hexanes is added over a 30 to 60-minute period while maintaining the temperature below 0°C. Stirring is continued for an additional 1 30 to 1.5 hours at -15° to 0°C and the mixture cooled to -35° to -15°C.

In a separate 5-L flask, 0.7 kg (1.54 mol) 5-(4-fluorophenyl)-2-(1-methylethyl)-1-(3-oxopropyl)-N,4-diphenyl-lH-pyrrole-3-carboxamide is dissolved 35 with 2.0 L of dry tetrahydrofuran, cooled to 0° to 2 3 8 8 -5°C, and added to the anion solution over a 30 to 45 minute period. The reaction is stirred at -20° to -15°C for 30 to 45 minutes, then quenched by the addition of 4 L aqueous 2 N hydrochloric acid solution over 5 to 15 seconds while stirring rapidly. After stopping the agitation, the lower layer is separated and the remaining organic layer washed with 4 L saturated aqueous sodium chloride.

Step F: Preparation of cjs-(4-Fluorophenyl)-P,5-dihydroxy-5-(1-methylethyl)-3-phenyl-4-f(phenylamino)c arbonyl1-lg-pyrrole-1-heptanoic acid, methyl ester.

The reaction solution obtained from Step E contained in a 50-L jacketed glass still is concentrated by vacuum distillation to a thick oil, then dissolved with 19 L tetrahydrofuran and cooled to 0°C under an air atmosphere. Triethylborane, a 1 molar solution in hexanes, (3.20 L, 1.4 equivalents based on Step E) is added over a 10 minute period, the atmosphere on the flask is switched to nitrogen, and the flask cooled to -105°C over 3.5 hours. During this period two liters of methanol are added when the temperature reaches -67°C. Powdered sodium borohydride (184 g, 4.8 mol) is added in 20 to 50-g portions over 1.5 hours, and the reaction maintained between -95° and -106°C for 13 hours, then between -60° and -100°C for 10 hours. Unreacted sodium borohydride is quenched by the addition of 750 mL (12.7 mol) acetic acid in 50-mL portions over a 45 minute period with a substantial amount of gas evolution and with a temperature rise from -60° to -40°C. Further quenching is accomplished by the addition of a solution of 1.0 L 30% hydrogen peroxide (9.7 mol), 3.0 L water, and 100 g dihydrogen sodium phosphate over a 15 minute period and is accompanied 23 8 0 4 4" by a temperature rise from -40° to 0°C. The reaction is allowed to warm to room temperature overnight, then the lower layer separated off and the upper layer washed with 4.0 L of saturated aqueous sodium chloride 5 solution.

Variation of Step F: Preparation of cj,s-2-(4-Fluoro-phenyl)-3,6-dihydroxy-5-(1-methylethyl)-3-phenyl-4-(phenylamino)carbonyl-ig-pyrrole-1-heptanoic acid, methyl ester.

The reaction solution obtained from Step E is concentrated under vacuum to a volume of 5 to 8 L, then dissolved in 20 L tetrahydrofuran and 4 L methanol under a nitrogen atmosphere. This solution is cooled to -85°C and 2.1 L of a 15% solution of 15 methoxydiethylborane in tetrahydrofuran (2.1 mol, 1.0 equivalent based on Step E) is added. The reaction is cooled to -97°C over one hour and 144 g (3.78 mol) of sodium borohydride added in 20 to 50-g portions over 1.5 hours. The reaction is maintained 20 between -93° and -97°C for 13 hours and allowed to warm to room temperature and stand for 60 hours under a nitrogen atmosphere.

The reaction is quenched by the addition of 460 mL (7.9 mol) acetic acid and concentrated by 25 vacuum distillation to an oil. The residue is dissolved with 8 L methanol, concentrated by vacuum distillation, redissolved with 8 L methanol, and reconcentrated by vacuum distillation to a volume of 6 L. The solution is diluted with 8 L tetrahydro-30 furan, 4 L hexanes, and carried into the next step. 2388 Step G: Preparation of trans-(t)-5-(4-Fluorophenvl)-2-(l-methylethvl)-N,4-diphenyl-l- T2-(tetra-hydro-4-hydroxy-6-oxo-2H-pyran-2-yl)ethyl1-lH-pyrrole-3-carboxamide.

The crude reaction mixture of Step F is cooled to 8°C, 8.0 L of 2.0 N aqueous sodium hydroxide is added, and the reaction stirred for 2 hours at 15° to 18°C. The reaction is diluted with 12 L water and the upper layer removed. The remaining aqueous layer is washed with 8 L hexanes then 8 L ethyl acetate is added followed by 1 L concentrated aqueous hydrochloric acid solution. The well-stirred mixture is allowed to separate, the lower layer discarded, and the upper layer washed four times with 4 L each of 2 N aqueous hydrochloric acid solution.

The ethyl acetate layer is concentrated to a foamy syrup by vacuum distillation, and the residue dissolved in 8 L toluene. The toluene is concentrated by vacuum distillation to a volume of 6 L, then allowed to stand at room temperature for 16 hours. The resulting thick slurry is filtered on a Buchner funnel, washed with 1 L of cool toluene, washed with 2 L of hexanes, and dried in a vacuum oven for 24 hours at room temperature, resulting in 686 g of trans-(±)-5-(4-fluorophenyl)-2-(1-methylethyl)-N,4-diphenyl-1-[2-(tetrahydro-4-hydroxy-6-oxo-2H-pyran-2-yl)ethyl)-lH-pyrrole-3-carboxamide. The filtrates are washed with 2 N aqueous hydrochloric acid solution and concentrated in vacuo to a volume of 2 L, then allowed to stand at room temperature for three days. The resulting solid is filtered, washed, and dried as before, resulting in 157 g of trans-(±)-5-(4-fluorophenyl )-2-(1-methylethyl)-N,4-diphenyl-l-[2-(tetra-hydro-4-hydroxy-6-oxo-2H-pyran-2-yl)ethyl)-lH-pyrrole-3-carboxamide. HPLC of the solids indicates 95% trans with 1.3% cis lactone for the first crop and 95% trans with 2.3% cis lactone for the second crop. The two crops of solid are dissolved in 8 L ethyl acetate by heating to 50 to 60°C, then filtered through a Buchner funnel along with 8 L of hexanes which has 5 been warmed to 50°C. The solution is allowed to cool to room temperature over 16 hours, the resulting slurry filtered through a Buchner funnel, and the solid washed with 2 L hexanes. The resulting solid is dried in a vacuum oven for 24 hours at room 10 temperature, resulting in 720 g of trans-(±)-5-(4-fluorophenyl)-2-(1-methylethyl)-N,4-diphenyl-l-[2-(tetrahydro-4-hydroxy-6-oxo-2H-pyran-2-yl)ethyl)-lH-pyrrole-3-carboxamide with a 98%:0.9% trans:cis HPLC assay. The second crop obtained by concentration as 15 before is approximately 100 g.

METHOD B Step A: Preparation of (±)-cis-6-(2-aminoethyl)-2,2-dimethyl-1,3-dioxane-4-acetic acid.

A solution of 1.04 g (4.88 mmol) of (±)-cis-6-20 (cyanomethyl)-2,2-dimethyl-l,3-dioxane-4-acetic acid in 100 mL of methanol saturated with anhydrous ammonia is added to a Parr shaker bottle containing 0.53 g of water wet Raney nickel #30. The solution is heated at' 45°C and 50 psig hydrogen pressure for 17 hours. The 25 suspension is cooled and filtered to remove the Raney nickel through filter aid and the precipitate washed with methanol. The filtrate is concentrated at reduced pressure. The residue is dissolved in methanol saturated with anhydrous ammonia treated with 30 decolorizing charcoal, filtered through filter aid and evaporated to give 0.56 g of (±)-cis-6-(2-amino-ethyl)-2,2-dimethyl-l,3-dioxane-4-acetic acid, mp 165° with decomposition at 169°C. 23 8 8 4 Fourier Transform Infrared (FTIR) (KBr): 1201.1, 1399.2, 1561.2, 2924.4, 3569.9 cm"1. 1H-NMR (D20, 200 MHz) 6 0.84 (m, 1H), 0.96 (s, 3H), 1.11 (s, 3H), 1.2-1.5 (m, 3H), 1.84 (dd, 1H, J = 5 14.0 Hz, J = 6.6 Hz), 1.99 (dd, 1H, J = 14.0 Hz, J = 6.8 Hz), 2.68 (t, 2H, J = 7.2 Hz), 3.6-3.85 (m, 1H), 3.85-4.15 (m, 1H). 13C-NMR (D20, 50 MHz) 6 19.64, 29.32, 32.94, 35.86, 36.95, 44.73, 68.16, 68.25, 100.18, 178.56.

Mass Spectrum (GC/MS), m/z 202, 198, 173, 142, 138, 120, 97, 82, 59, 43.

Step B: Preparation of Trang-(±)-5-(4-fluorophenyl)-2-(1-methylethyl)-N,4-diphenyl-l-f 2-(tetra-hydro-4-hydroxy-6-oxo-2H-pyran-2-yl)ethyl1-15 lg-pyrrole-3-carboxamide.

A solution of 0.26 g (1.21 mmol) of (±)-cis-6-(2-aminoethyl)-2,2-dimethyl-l,3-dioxane-4-acetic acid and 0.504 g (1.20 mmol) of (±)4-fluoro-a-[2-methyl-l-oxopropyl ]-y-oxo-N,p-diphenylbenzenebutaneamide 20 mixture of [R-(R*,R*)], [R-(R*,S*)], [S-(R*,R*)] and [S-(R*,S*)] isomers in 5 mL of dimethyl sulfoxide is heated at 105°C for 15 hours. The solution is cooled and poured into 100 mL of diethyl ether and 50 mL of saturated ammonium chloride in water. The layers are 25 separated and the organic layer washed with water (2 x 50 mL) and 5% sodium hydroxide solution (2 x 100 mL - to extract the intermediate from unreacted diketone). The aqueous layer is acidified with dilute hydrochloric acid solution and extracted 30 with 30 mL of ethyl acetate. A drop of concentrated hydrochloric acid is added to the ethyl acetate solution and allowed to stand 18 hours. The solution 2388 is concentrated in vacuo and the concentrate is redissolved in 30 mL of ethyl acetate and treated with one drop of concentrated hydrochloric acid. The solution is stirred two hours, concentrated in vacuo, and dissolved in 6 mL of toluene. Trans-(±)-5-(4-fluorophenyl )-2- (1-methylethyl)-N, 4-diphenyl-l- [2-(tetrahydro-4-hydroxy-6-oxo-2H-pyran-2-yl) ethyl ] -1H-pyrrole-3-carboxamide crystallizes and is isolated by filtration. A total of 0.16 g of trans-(±)-5-(4-f luorophenyl) -2-(1-methylethyl )-N, 4-diphenyl-l- [2-(tetrahydro-4-hydroxy-6-oxo-2H-pyran-2-yl) ethyl ] -1H-pyrrole-3-carboxamide is isolated in two crops.

METHOD C Step A: Preparation of either (±)-(2a,43,63) or (±)-(2a,4a,63)-2-phenyl-6-(2-propenyl)-1,3-dioxane-4-acetonitrile.

Potassium cyanide, 1.3 g (20 mmol), is added to a room temperature solution of (R*,R*)-a-2-propenyl-oxiraneethanol, 2.56 g (20 mmol), in 25 mL of 4:1 isopropanol:water. The solution is stirred for 20 hours at ambient temperature, concentrated, and partitioned between ethyl acetate and brine. The aqueous layer is extracted 2x with ethyl acetate and • the combined ethyl acetate extracts are washed with brine and dried (magnesium sulfate). The crude product is dissolved in 20 mL of benzaldehyde dimethyl acetal, camphorsulfonic acid is added, and the solution is stirred for 18 hours at room temperature. Concentration and flash chromatography after concentration in vacuo provides 1.30 g of either (±)-(2a,4p,63) or (±)-(2a,4a,63)-2-phenyl-6-(2-propenyl )-l, 3-dioxane-4-acetonitrile 238844 200 MHz NMR (CDC13) 6 1.48 (m, 1H), 1.71 (m, 1H), 2.41 (m, 2H), 2.58 (m, 2H), 3.87 (m, 1H), 4.03 (m, 1H), 5.1 - 5.2 (m, 2H), 5.53 (s, 1H), 5.87 (m, 1H), 7.3 - 7.6 (m, 5H) 13C-NMR (CDC13/ 50 MHz) 5 24.23, 35.07, 39.79, 71.57, 75.48, 100.44, 116.37, 117.53, 125.89, 127.91, 128.61, 133.05, 137.71.

GC/MS m/e 243 (M+), 242, 203, 202, 120, 107, 105, 79, 75, 41.

FTIR (neat) 699.6, 758.7, 920.8, 1028.8, 1051.9, 1121.4, 1345.2, 1383.7, 1401.7, 2253.1, 2916.6 cm-1.

Step B; Preparation of either (±)-(2a,4a,6a) or (±)-(2a,4g,6g)-6-(2-oxoethyl)-2-phenyl-1,3-dioxane-4-acetonitrile.

A solution of either (±)-(2a,4p,6p) or (±)-(2a,4a,6a)-2-phenyl-6-(2-propenyl)-l,3-dioxane-4-acetonitrile, 4.11 g (16.89 mmol), in 100 mL of dichloromethane is cooled to -78°C under nitrogen.

Ozone (Welsbach generator, flow rate 0.1, voltage = 20 90V) is then passed through a fritted gas inlet tube into the solution until the blue color of ozone appears. The current is turned off, and oxygen - * bubbled through until the blue color is discharged.

Triphenylphosphine, 4.87 g (18.58 mmol), is added and 25 the colorless solution is allowed to warm to room temperature. Flash chromatography provides after concentration in vacuo 3.75 g of pure either (±)-(2a,4a,6a) or (±)-(2a,40,60)-6-(2-oxoethyl)-2-phenyl-1,3-dioxane-4-acetonitrile.

GC/MS m/e 245 (M+), 244, 123, 105, 95, 94, 77, 51, 41. 238844 Step C: Preparation of either (±)-(2a,4a,6a) or (±)-(2a,40,60 )-6-(cyanomethyl)-2-phenyl-1,3-dioxane-4-acetic acid.

Jones reagent (chromium trioxide-sulfuric acid-water), 3.8 mL (97.6 mmol), is added dropwise to a 0°C solution of either (±)-(2a,4a,6a) or (±)-(2a,40,60)-6-(2-oxoethyl)-2-phenyl-l,3-dioxane-4-acetonitrile, 1.86 g (7.6 mmol), dissolved in 50 mL of acetone until the orange color is not discharged.

After stirring a further 15 minutes, the mixture is poured into 300 mL of diethyl ether and washed with brine until the aqueous washes are colorless. The diethyl ether layer is dried (magnesium sulfate), filtered, and concentrated to provide 1.84 g of either (±)-(2a,4a,6a) or (±)-(2a-(2a,40,60)-6-(cyanomethyl)-2-phenyl-l,3-dioxane-4-acetic acid as a yellow gum. 200 MHz NMR (CDCl3) 6 1.61 (m, 1H), 2.04 (m, 1H), 2.6 - 2.8 (m, 3H) 2.82 (dd, 1H, J = 15.9 Hz, J = 7.1 Hz), 4.20 (m, 1H), 4.40 (m, 1H), 5.60 (s, 1H), 7.2 - 7.5 (m, 5H).

Step D: Preparation of either (±)-(2a,4a,6a) or (±)-(2a,40,60)-6-(2-aminoethyl)-2-phenyl-l,3-dioxane-4-acetic acid.

A solution of 0.16 g (0.61 mmol) of either (±)-(2a-4a,6a) or (±)-(2a,40,60)-6-(cyanomethyl)-2-phenyl-1,3-dioxane-4-acetic acid in 50 mL of methanol saturated with anhydrous ammonia is added to a Parr shaker bottle containing 0.2 g of water wet Raney nickel #30. The solution is heated at 40°C and 50 pounds per square inch gage (psig) hydrogen pressure for 6 hours. The suspension is cooled and filtered to remove the Raney nickel through filter aid and the precipitate washed with methanol. The filtrate is concentrated at reduced pressure. The 2 3 8 8 4 4, residue is dissolved in methanol saturated with anhydrous ammonia treated with decolorizing charcoal, filtered through filter aid and evaporated to give 0.11 g of either (±)-(2a,4a,6a) or (±)-(2a,40,60)-6-(2-aminoethyl)-2-phenyl-l,3-dioxane-4-acetic acid; mp 215.9 - 217.9°C with decomposition. 200 MHz NMR (D20) 6 1.4 - 1.9 (m, 4H), 2.39 (dd, 1H, J = 14.8 Hz, J = 6.7 Hz), 2.55 (dd, 1H, J = 14.8 Hz, J = 7.4 Hz), 2.73 (t, 2H, J = 7.2 Hz) 4.11 (m, 1H), 4.33 (m, 1H), 5.70 (s, 1H), 7.4 - 7.6 (m, 5H). 13C-NMR (D20, 50 MHz) 6 39.20, 39.78, 40.83, 47.11, 78.34, 78.73, 104.06, 129.15, 131.56, 132.38, 140.51, 181.89.

Step E: Preparation of Trans-(±)-5-(4-fluorophenyl)-2-(1-methylethyl)-N,4-diphenyl-l-[2-(tetra-hydro-4-hydroxy-6-oxo-2B-pyran-2-yl)ethyl1-lH-pyrrole-3-carboxamide.

A solution of 0.31 g (1.21 mmol) of either (±)-(2a,4a,6a) or (±)-(2a,40,60)-6-(2-aminoethyl)-2-phenyl-1,3-dioxane-4-acetic acid and 0.504 g (1.20 mmol) of (±)-4-fluoro-a-[2-methyl-l-oxopropyl]-V-oxo-N,0-diphenylbenzenebutaneamide mixture of [R-(R*,R*)], [R-(R*,S*)], [S-(R*,R*)] and [S-(R*,S*)] isomers in 5 mL of dimethyl sulfoxide is heated at 105°C for 15 hours. The solution is cooled and poured into 100 mL of diethyl ether and 50 mL of saturated ammonium chloride in water. The layers are separated and the organic layer washed with water (2 x 50 mL) and 5% sodium hydroxide solution (2 x 100 mL - to extract the intermediate from unreacted diketone). The aqueous layer is acidified with dilute hydrochloric acid solution, stirred for three hours and extracted with 30 mL of ethyl acetate. A drop of concentrated 23 8 8 4 hydrochloric acid is added to the ethyl acetate solution, the solution is stirred and allowed to stand 18 hours. The solution is concentrated in vacuo and the concentrate is redissolved in 30 mL of ethyl acetate and treated with one drop of concentrated hydrochloric acid. The solution is stirred two hours, concentrated in vacuo, and dissolved in 6 mL of toluene. Trans-(±)-5-(4-fluorophenyl)-2-(1-methylethyl )-N, 4-diphenyl-l- [2-(tetrahydro-4-hydroxy-6-oxo-2H-pyran-2 -yl) ethyl ] -lH-pyrrole-3 -carboxamide crystallizes and is isolated by filtration. A total of 0.16 g of trans-(±)-5-(4-fluorophenyl)-2-(1-methyl-ethyl )-N, 4-diphenyl-l- [2 - (tetrahydro-4-hydroxy-6-oxo-2H-pyran-2-yl)ethyl]-lH-pyrrole-3-carboxamide is isolated in two crops.

METHOD D Step A: Preparation of (±)-cjs-9-(2-propenyl)-6,10-dioxaspiro [4.5]decane-7-acetonitrile.

Potassium cyanide, 1.3 g (20 mmol), is added to a room temperature solution of (R*,R*)-a-2-propenyl-oxiraneethanol, 2.56 g (20 mmol), in 25 mL of 4:1 isopropanol:water. The solution is stirred for 20 hours at ambient temperature, concentrated, and partitioned between'ethyl acetate and brine. The aqueous layer is extracted 2x with ethyl acetate and the combined ethyl acetate extracts are washed with brine and dried (magnesium sulfate). The crude product is dissolved in 20 mL of 1,1-dimethoxycyclo-pentane, camphorsulfonic acid is added, and the solution is stirred for 18 hours at room temperature. Concentration and flash chromatography after concentration in vacuo provides 1.40 g of (±)-cis-9-(2-propenyl)-6,10-dioxaspiro[4.5]decane-7-acetonitrile. 2 3 8 8 4 4 O Step B: Preparation of (±)-cjs-9-(2-oxoethvl)-6,10-dioxaspiro [4.5]decane-7-acetonitrile.

A solution of (±)-cis-9-(2-propenyl)-6,10-dioxa-O spiro[4.5]decane-7-acetonitrile, 3.4 g (15.36 mmol), in 100 mL of dichloromethane is cooled to -78°C under nitrogen. Ozone (Welsbach generator, flow rate 0.1, voltage = 90V) is then passed through a fritted gas inlet tube into the solution until the blue color of ozone appears. The current is turned off, and oxygen 10 bubbled through until the blue color is discharged.

Triphenylphosphine, 4.2 g (16 mmol), is added and the colorless solution is allowed to warm to room temperature. Flash chromatography provides after concentration in vacuo 2.5 g of pure (±)-cis-9-(2-oxo-15 ethyl) -6,10-dioxaspiro [4.5] decane-7-acetonitrile.

Step C: Preparation of (±)-cis-9-(cyanomethyl)-6,10-dioxaspiro[4.51decane-7-acetic acid.

Jones reagent (chromium trioxide-sulfuric acid-water), 3.8 mL (97.6 mmol), is added dropwise to 20 a 0°C solution of (±)-cis-9-(2-oxoethyl)-6-10-dioxa-spiro[4.5]decane-2-acetonitrile 1.70 (7.6 mmol), dissolved in 50 mL of acetone until the orange color is not discharged. After stirring a further 15 minutes, the mixture is poured into 300 mL of 25 diethyl ether and washed with brine until the aqueous washes are colorless. The diethyl ether layer is dried (magnesium sulfate), filtered, and concentrated to provide 1.20 g of (±)-cis-9-(cyanomethyl)-6,10-dioxaspiro[4.5]decane-7-acetic acid as a colorless 30 solid.

Step D: Preparation of (±)-cis-9-(2-aminoethyl)-6,10-dioxaspiro[4.5]decane-7-acetic acid. A solution of 1.17 g (4.88 mmol) of (±)-cis-9-(cyanomethyl)-6,10-dioxaspiro[4.5]decane-7-acetic acid 238 8 in 100 mL of methanol saturated with anhydrous ammonia is added to a Parr shaker bottle containing 0.53 g of water wet Raney nickel #30. The solution is heated at 45°C and 50 pounds per square inch gage (psig) hydrogen pressure for 17 hours. The suspension is cooled and filtered to remove the Raney nickel through filter aid and the precipitate is washed with methanol. The filtrate is concentrated at reduced pressure. The residue is dissolved in methanol saturated with anhydrous ammonia treated with decolorizing charcoal, filtered through filter aid and evaporated to give 0.56 g of (±)-cis-9-(2-aminoethyl)-6,10-dioxaspiro-[4.5]decane-7-acetic acid.

Step E: Preparation of Xrans-(±)-5-(4-fluorophenyl)-2-(1-methylethyl) -N, 4-diphenyl-l-[2-(tetra-hydro-4-hydroxy- 6-oxo-2H-pyr an-2 -y 1) ethyl 1 -lg-pyrrole-3-carboxamide.

A solution of 0.295 g (1.21 mmol) of (±)-cis-9-(2-aminoethyl) -6,10-dioxaspiro [4.5] decane-7-acetic acid and 0.504 g (1.20 mmol) of (±)-4-fluoro-a-[2-methyl-l-oxopropyl] -y-oxo-N, p-diphenylbenzenebutane-amide mixture of [R-(R*,R*)], [R-(R*,S*) ] , [S-(R*,R*)] and [S-(R*,S*)] isomers in 5 mL of dimethyl sulfoxide is heated at 105°C for 15 hours. The solution is cooled and poured into 100 mL of diethyl ether and 50 mL of saturated ammonium chloride in water. The layers are separated and the organic layer washed with water (2 x 50 mL) and 5% sodium hydroxide solution (2 x 100 mL - to extract the intermediate protected acid from unreacted diketone). The aqueous layer is acidified with dilute hydrochloric acid solution, stirred for three hours and extracted with 30 mL of ethyl acetate. A drop of concentrated hydrochloric acid is added to the ethyl acetate solution and allowed to stand 18 hours. The solution is 2 3 3 8 4 concentrated in vacuo and the concentrate is redissolved in 30 mL of ethyl acetate and treated with one drop of concentrated hydrochloric acid. The solution is stirred two hours, concentrated in vacuo, and dissolved in 6 mL of toluene. Trans-(±)-5-(4-fluorophenyl)-2-(1-methylethyl)-N,4-diphenyl-l-[2-(tetrahydro-4-hydroxy-6-oxo-2H-pyran-2-yl) ethyl ] -1H-pyrrole-3-carboxamide crystallizes and is isolated by filtration. A total of 0.16 g of trans-(±)-5-(4-f luorophenyl )-2- (1-methylethyl) -N, 4-diphenyl-l- [2-(tetrahydro-4-hydroxy-6-oxo-2H-pyran-2-yl) ethyl ] -1H-pyrrole-3-carboxamide is isolated in two crops.

METHOD E Step A; Preparation of (±)-cis-4-(2-propenyl)-1,5-dioxaspiro[5,5]undecane-2-acetonitrile.

Potassium cyanide, 1.3 g (20 mmol), is added to a room temperature solution of (R*,R*)-a-2-propenyl-oxiraneethanol, 2.56 g (20 mmol), in 25 mL of 4:1 isopropanol:water. The solution is stirred for 20 hours at ambient temperature, concentrated, and partitioned between ethyl acetate and brine. The aqueous layer is extracted 2x with ethyl acetate and the combined ethyl acetate extracts are washed with brine and dried (magnesium sulfate). The crude product is dissolved in 20 mL of 2,2-dimethoxycyclo-hexane, camphorsulfonic acid is added, and the solution is stirred for 18 hours at room temperature. Concentration and flash chromatography after concentration in vacuo provides 1.50 g of (±)-cis-4-(2-propenyl)-l,5-dioxaspiro[5.5]undecane-2-acetonitrile 200 MHz NMR (CDCl3) 5 1.26 (m, 1H), 1.4 - 1.7 (m, 8H), 1.87 (m, 2H), 2.1 - 2.3 (m, 2H), 2.51 (d, 2H, J = 6.05 o 238844 Hz), 3.95 (m, 1H), 4.15 (m, 1H), 5.0 - 5.2 (m, 2H), 5.83 (m, 1H). 13C-NMR (CDCI3, 50 MHz) 5 22.16, 24,71, 25.42, 28.20, 35.47, 38.33, 40.30, 64.08, 66.87, 98.84, 116.53, 5 116.83, 113.46.

GC/MS m/e 235 (m+), 206, 192, 120, 99, 93, 79, 69, 55, O 41 • FTIR (film) 964.5, 1121.4, 1160.0, 2253.1, 2937.2 cm"1.

Step B: Preparation of (±)-cjs-4-(2-oxoethyl)-l,5-dioxaspiro[5.5]undecane-2-acetonitrile.

A solution of (±)-cis-4-(2-propenyl)-l,5-dioxa-spiro[5.5]undecane-2-acetonitrile 4.26 g (19.42 mmol), in 100 mL of dichloromethane is cooled to -78 °C under nitrogen. Ozone (Welsbach generator, flow rate 0.1, voltage = 90V) is then passed through a fritted gas inlet tube into the solution until the blue color of ozone appears. The current is turned off, and oxygen bubbled through until the blue color is discharged.

Triphenylphosphine, 5.6 g (21.36 mmol), is added and the colorless solution is allowed to warm to room temperature. Flash chromatography provides after concentration in vacuo 4.04 g of pure (±)-cis-4-(2-oxo- * ethyl)-1,5-dioxaspiro[5.5]undecane-2-acetonitrile. 200 MHz NMR (CDC13) 6 1.3 - 2.0 (m, 12H), 2.5 - 2.7 (m, 4H), 4.20 (m, 1H), 4.36 (m, 1H), 9.81 (t, 1H, J = 1.74 Hz). 13C-NMR (CDC13, 50 MHz) 6 22.39, 22.44, 24.97, 25.59, 28.44, 35.82, 38.48, 49.54, 63.25, 64.17, 99.66, 116.57, 199.82. 238 8 4 4 GC/MS m/e 237 (m+), 208, 194, 122, 94, 81, 55, 42, 41.

FTIR (film) 969.6, 1069.9, 1126.5, 1160.0, 1368.3, 1386.3, 1728.4, 2934.6 cm"1.

Step C: Preparation of (±)-cis-4-(cyanomethyl)-1,5-5 dioxaspiro F5.5]undecane-2-acetic acid.

Jones reagent (chromium trioxide-sulfuric acid-water), 4.4 mL (8.85 mmol), is added dropwise to a 0°C solution of (±)-cis-4-(2-oxoethyl)-1,5-dioxa-spiro[5.5]undecane-2-acetonitrile, 3.62 g (12.6 mmol), 10 dissolved in 30 mL of acetone until the orange color is not discharged. After stirring a further 15 minutes, the mixture is poured into 300 mL of diethyl ether and washed with brine until the aqueous washes are colorless. The diethyl ether layer is 15 dried (magnesium sulfate), filtered, and concentrated to provide 3.65 g of (±)-cis-4-(cyanomethyl)-!,5-dioxaspiro[5.5]undecane-2-acetic acid as a yellow solid. 200 MHz NMR (Pyridine) 6 1.2 - 2.0 (m, 12H), 2.5 - 2.9 20 (m, 4H), 4.19 (m, 1H), 4.50 (m, 1H).

Step D: Preparation of (±)-cis-4-(2-aminoethyl)-1,5-dioxaspiro[5.5"lundecane-2-acetic acid. A solution of 0.13 g of (±)-cis-4-(cyanomethyl)-l,5-dioxaspiro[5.5]undecane-2-acetic acid in 20 mL of 25 methanol saturated with anhydrous ammonia is added to a Parr shaker bottle containing 0.2 g of water wet Raney nickel #30. The solution is heated at 40°C and 50 pounds per square inch gage (psig) hydrogen pressure for 17 hours. The suspension is cooled and 30 filtered to remove the Raney nickel through filter aid and the precipitate is washed with methanol. The filtrate is concentrated at reduced pressure. The 23884 4 • i residue is dissolved in methanol saturated with anhydrous ammonia, treated with decolorizing charcoal, filtered through filter aid and evaporated to give 0.13 g of (±)-cis-4-(2-aminoethyl)-1,5-dioxaspiro-5 [5.5]undecane-2-acetic acid. 200 MHz NMR (D20) 5 1.1 - 1.7 (m, 10H), 1.75 - 2.1 (m, 4H), 2.19 (dd, 1H, J = 14.6 Hz, J = 6.7 Hz), 2.31 (dd, 1H, J = 14.6 Hz, J = 7.3 Hz), 2.69 (t, 2H, J = 7.1 Hz), 4.09 (m, 1H), 4.34 (m, 1H). 13C-NMR (D20, 50 MHz) 5 24.50, 24.73, 27.55, 30.80, 38.91, 39.39, 39.63, 40.48, 47.03, 69.37, 69.54, 102.74, 181.33.

Step E: Preparation of Trans-(±)-5-(4-fluorophenyl)-2-(1-methylethyl)-N,4-diphenyl-l-[2-(tetra-15 hydro-4-hydroxy-6-oxo-2H-pyran-2-yl)ethyl]- lB-pyrrole-3-carboxamide.

A solution of 0.31 g (1.21 mmol) of (±)-cis-4-(2-aminoethyl)-l,5-dioxaspiro[5.5]undecane-2-acetic acid and 0.504 g (1.20 mmol) of (±)-4-fluoro-a-[2-20 methyl-l-oxopropyl]-y-oxo-N,p-diphenylbenzenebutane-amide mixture of [R-(R*,R*)], [R-(R*,S*)], [S-(R*,R*)] and [S-(R*,S*)] isomers in 5 mL of dimethyl sulfoxide is heated at 105°C for 15 hours. The solution is cooled and poured into 100 mL of diethyl ether and 25 50 mL of saturated ammonium chloride in water. The iayers are separated and the organic layer washed with water (2 x 50 mL) and 5% sodium hydroxide solution (2 x 100 mL - to extract the intermediate acid from unreacted diketone). The aqueous layer is acidified 30 with dilute hydrochloric acid solution, stirred for three hours and extracted with 30 mL of ethyl acetate to remove the protecting group before lactonization. The extract is concentrated and dissolved in 30 mL of ■x... 2 3-8 8 4 4 ethyl acetate. A drop of concentrated hydrochloric acid is added to the ethyl acetate solution and allowed to stand 18 hours. The solution is ■*rJ concentrated in vacuo and the concentrate is redissolved in 30 mL of ethyl acetate and treated with one drop of concentrated hydrochloric acid. The solution is stirred two hours, concentrated in vacuo, and dissolved in 6 mL of toluene. Trans-(±)-5-(4-O fluoro-phenyl)-2-(1-methylethyl) -N, 4-diphenyl-l- [2- (tetrahydro-4-hydroxy-6-oxo-2H-pyran-2-yl)ethyl]-lH-pyrrole-3-carboxamide crystallizes and is isolated by filtration. A total of 0.155 g of trans-(±)-5-(4-fluorophenyl) -2- (1-methylethyl) -N, 4-diphenyl-l- [2-(tetrahydro-4-hydroxy-6-oxo-2H-pyran-2-yl) ethyl] -1H-15 pyrrole-3-carboxamide is isolated in two crops.

METHOD F Step A: Preparation of (±)-cjs-6-(2-propenyl)-l,3-dioxane-4-acetonitrile Potassium cyanide, 1.3 g (20 mmol), is added to a 20 room temperature solution of (R*,R*)-a-2-propenyl-oxiraneethanol, 2.56 g (20 mmol), in 25 mL of 4:1 isopropanol:water. The solution is stirred for 20 hours at ambient temperature, concentrated, and partitioned between ethyl acetate and brine. The _2$ aqueous layer is extracted 2x with ethyl acetate and the combined ethyl acetate extracts are washed with brine and dried (magnesium sulfate). The crude product is dissolved in 20 mL of dimethoxymethane, camphorsulfonic acid is added, and the solution is 30 stirred for 18 hours at room temperature.

Concentration and flash chromatography after concentration in vacuo provides 1.20 g of (±)-cis-6-(2-propenyl)-1, 3-dioxane-4-acetonitrile. 238844 Step B: Preparation of (±)-cis-6-(2-oxoethyl)-l,3-dioxane-4-acetonitrile.

A solution of (±)-cis-6-(2-propenyl)-1,3-dioxane-4-acetonitrile, 2.57 g (15.36 mmol), in 100 mL of dichloromethane is cooled to -78°C under nitrogen. Ozone (Welsbach generator, flow rate 0.1, voltage = 90V) is then passed through a fritted gas inlet tube into the solution until the blue color of ozone appears. The current is turned off, and oxygen bubbled through until the blue color is discharged. Triphenylphosphine, 4.87 g (28.58 mmol), is added and the colorless solution is allowed to warm to room temperature. Flash chromatography provides after concentration in vacuo 2.3 g of pure (±)-cis-6-(2-oxo-ethyl)-1,3-dioxane-4-acetonitrile.

Step C: Preparation of (±)-cis-6-(cyanomethyl)-l,3-dioxane-4-acetic acid.

Jones reagent (chromium trioxide-sulfuric acid-water), 3.8 mL (97.6 mmol), is added dropwise to a 0°C solution of (±)-cis-6-(2-oxoethyl)-l,3-dioxane-4-acetonitrile, 1.29 g (7.6 mmol), dissolved in 50 mL of acetone until the orange color is not discharged. After stirring a further 15 minutes, the mixture is poured into 300 mL of diethyl ether and washed with brine until the aqueous washes are colorless. The diethyl ether layer is dried (magnesium sulfate), filtered, and concentrated to provide 1.2 g of (±)-cis-6-(cyanomethyl)-l,3-dioxane-4-acetic acid as a colorless solid.

Step D: Preparation of (±)-cis-6-(2-aminoethvl)-l,3-dioxane-4-acetic acid.

A solution of 1.04 g (4.88 mmol) of (±)-cis-6-(cyanomethyl)-l,3-dioxane-4-acetic acid in 100 mL of methanol saturated with anhydrous ammonia is added to a Parr shaker bottle containing 0.53 g of water wet Raney nickel #30. The solution is heated at 45°C and 50 pounds per square inch gage (psig) hydrogen pressure for 17 hours. The suspension is cooled and 5 filtered to remove the Raney nickel through filter aid and the precipitate washed with methanol. The filtrate is concentrated at reduced pressure. The residue is dissolved in methanol saturated with anhydrous ammonia, treated with decolorizing charcoal, 10 filtered through filter aid and evaporated to give 0.56 g of (±)-cis-6-(2-aminoethyl)-1,3-dioxane-4-acetic acid.

Step E: Preparation of Trans-(±)-5-(4-fluorophenyl)-2- (1-methylethyl) -N, 4-diphenyl-l- [2- (tetra-15 hydro-4-hydroxy-6-oxo-2B-pyran-2-yl) ethyl 1 - lH-pyrrole-3-carboxamide.

A solution of 0.26 g (1.21 mmol) of (±)-cis-6-(2-aminoethyl)-l,3-dioxane-4-acetic acid and 0.504 g (1.20 mmol) of (±)-4-fluoro-a-[2-methyl-l-oxopropyl]-20 y-oxo-N, 0-diphenylbenzenebutaneamide in 5 mL of dimethyl sulfoxide is heated at 105°C for 15 hours. The solution is cooled and poured into 100 mL of diethyl ether and 50 mL of saturated ammonium chloride in water. The layers are separated and the organic 25 layer washed with water (2 x 50 mL) and 5% sodium hydroxide solution (2 x 100 mL - to extract the intermediate acid from unreacted dike tone). The aqueous layer is acidified with dilute hydrochloric acid solution and extracted with 30 mL of ethyl 30 acetate. A drop of concentrated hydrochloric acid is added to the ethyl acetate solution and allowed to stand 18 hours. The solution is concentrated in vacuo and the concentrate is redissolved in 30 mL of ethyl acetate and treated with one drop of concentrated 35 hydrochloric acid. The solution is stirred two hours, 238844 concentrated in vacuo, and dissolved in 6 mL of toluene. Trans-(±)-5-(4-fluorophenyl)-2-(1-methylethyl )-N, 4-diphenyl-l- [2-(tetrahydro-4-hydroxy-6-oxo-2H-pyran-2-yl)ethyl]-lH-pyrrole-3-carboxamide crystallizes and is isolated by filtration. A total of 0.15 g of trans- (±) -5- (4-f luorophenyl) -2- (1-m.ethvl-ethyl )-N, 4-diphenyl-l- [2-(tetrahydro-4-hydroxy-6-oxo-2H-pyran-2-yl)ethyl ] -lH-pyrrole-3-carboxamide is isolated in two crops.

METHOD G Step A: Preparation of either (±)-(2a,43, 63) or (±)-(2a,4a,6a)-2-methyl-6-(2-propenyl)-1,3-dioxane-4-acetonitrile.

Potassium cyanide, 1.3 g (20 mmol), is added to a room temperature solution of (R*,R*)-a-2-propenyl-oxiraneethanol, 2.56 g (20 mmol), in 25 mL of 4:1 isopropanol:water. The solution is stirred for 20 hours at ambient temperature, concentrated, and partitioned between ethyl acetate and brine. The aqueous layer is extracted 2x with ethyl acetate and the combined ethyl acetate extracts are washed with brine and dried (magnesium sulfate). The crude product is dissolved in 20 mL of 1,1-dimethoxyethane, camphorsulfonic acid is added, and the solution is stirred for 18 hours at room temperature. Concentration and flash chromatography after concentration in vacuo provides 1.30 g of either (±)-(2a,43,6p) or (±)-(2a,4a,6a)-2-methyl-6-(2-propenyl)-l,3-dioxane-4-acetonitrile. 23 8 Step B: Preparation of either (±)-(2a,4a,6a ) or (±)-(2a,4g , 6ft)-2-methyl-6-(2-oxoethyl)-l,3-dioxane-4-acetonitrile.

A solution of either (±)-(2a,4ft,6p) or (±)-(2a,4a,6a)-2-methyl-6-(2-propenyl)-1,3-dioxane-4-acetonitrile, 2.78 g (15.36 mmol), in 100 mL of dichloromethane is cooled to -78°C under nitrogen. Ozone (Welsbach generator, flow rate 0.1, voltage = 90V) is then passed through a fritted gas inlet tube into the solution until the blue color of ozone appears. The current is turned off, and oxygen bubbled through until the blue color is discharged. Triphenylphosphine, 4.2 g (16 mmol), is added and the colorless solution is allowed to warm to room temperature. Flash chromatography provides after concentration in vacuo 2.5 g of pure either (±)-(2a,4a,6a) or (±)-(2a,4p,6p)-2-methyl-6-(2-oxoethyl )-1,3-dioxane-4-acetonitrile.

Step C: Preparation of either (±)-(2a,4a,6a) or (±)-(2a,43,6ft)-6-(cyanomethyl)-2-methyl-1,3-dioxane-4-acetic acid.

Jones reagent (chromium trioxide-sulfuric acid-water), 3.8 mL (7.6 mmol), is added dropwise to a 0°C solution of either (±)-(2a,4a,6a) or (±) - (2a, 4ft, 6p )-2-methyl-6-(2-oxoethyl)-1,3-dioxane-4-acetonitrile, 1.40 g (7.6 mmol), dissolved in 50 mL of acetone until the orange color is not discharged. After stirring a further 15 minutes, the mixture is poured into 300 mL of diethyl ether and washed with brine until the aqueous washes are colorless. The diethyl ether layer is dried (magnesium sulfate), filtered, and concentrated to provide 1.01 g of either (±)-(2a,4a,6a) or (±)-(2a,4p,6p)-6-(cyanomethyl)-2-methyl-l,3-dioxane-4-acetic acid as a colorless solid. 23 8 8 Step D: Preparation of either (±)-(2a,4a,6a ) or (±)-(2a,4g,63)-6-(2-aminoethyl)-2-methyl-1,3-dioxane-4-acetic acid.

A solution of 0.97 g (4.88 mmol) of either 5 (±)-(2a,4a,6a) or (±)-(2a,4p, 6p)-6-(cyanomethyl)-2-methyl-l,3-dioxane-4-acetic acid in 100 mL of methanol saturated with anhydrous ammonia is added to a Parr shaker bottle containing 0.53 g of water wet Raney nickel #30. The solution is heated at 45°C and 10 50 pounds per square inch gage (psig) hydrogen pressure for 17 hours. The suspension is cooled and filtered to remove the Raney nickel through filter aid and the precipitate washed with methanol. The filtrate is concentrated at reduced pressure. The 15 residue is dissolved in methanol saturated with anhydrous ammonia treated with decolorizing charcoal, filtered through filter aid and evaporated to give 0.50 g of either (±)-(2a,4a,6a) or (±)-(2a,4p,63)-6-(2-aminoethyl)-2-methyl-l,3-dioxane-4-acetic acid.

Step E: Preparation of Tgmns-(±)-5-(4-fluorophenyl)-2-(1-methylethyl)-N,4-diphenyl-l-f2-(tetra-hydro-4-hydroxy-6-oxo-2g-pyran-2-yl)ethyl]-lg-pyrrole-3-carboxamide.

A solution of 0.31 g of either (±)-(2a,4a,6a) or 25 (±)-(2a,4p,60)-6-(2-aminoethyl)-2-methyl-l,3-dioxane-4-acetic acid and 0.504 g (1.20 mmol) of (±)-4-fluoro-a-[2-methyl-l-oxopropyl]-y-oxo-N, p-diphenylbenzene-butaneamide in 5 mL of dimethyl sulfoxide is heated at 105°C for 15 hours. The solution is cooled and poured 30 into 100 mL of diethyl ether and 50 mL of saturated ammonium chloride in water. The layers are separated and the organic layer washed with water (2 x 50 mL) and 5% sodium hydroxide solution (2 x 100 mL - to extract the intermediate acid from unreacted diketone). 35 The aqueous layer is acidified with dilute hydrochloric • "*v- 238 acid solution, stirred for three hours and extracted with 30 mL of ethyl acetate. A drop of concentrated hydrochloric acid is added to the ethyl acetate solution and allowed to stand 18 hours. The solution 5 is concentrated in vacuo and the concentrate is redissolved in 30 mL of ethyl acetate and treated with one drop of concentrated hydrochloric acid. The solution is stirred two hours, concentrated in vacuo, and dissolved in 6 mL of toluene. Trans-(±)-5-(4-10 f luorophenyl) -2- (1-methylethyl) -N, 4-diphenyl-l- [2-(tetrahydro-4-hydroxy-6-oxo-2H-pyran-2-yl) ethyl ] -1H-pyrrole-3-carboxamide crystallizes and is isolated by filtration. A total of 0.14 g of trans-(±)-5-(4-fluorophenyl) -2- (1-methylethyl )-N, 4-diphenyl-l- [2-15 (tetrahydro-4-hydroxy-6-oxo-2H-pyran-2-yl) ethyl j-lH-pyrrole-3-carboxamide is isolated in two crops.

METHOD H Step A: Preparation of (±)-cis-l,1-dimethylethyl 6-(cyanomethyl)-2,2-dimethyl-l,3-dioxane-4-20 acetate. (±)-cis-6-(cyanomethyl) -2,2-dimethyl-l, 3-dioxane-4-acetic acid (3.72 g, 17.44 mmol) is dissolved in 20 mL of dichloromethane, cooled to 0°C and 0.2 g of 4-dimethylaminopyridine (DMAP) is added, followed by - 25 t-butyl alcohol and followed by 4.32 g of dicyclohexylcarbodiimide (DCC). This solution is allowed to slowly warm to room temperature over a 76.5-hour period. Thin layer chromotography (TLC) shows mainly product, and some slightly lower Rf by-products. The 30 mixture is stirred one hour and 50 mL of dichloro-methane is added and stirring continues five hours. An additional 100 mL of diethyl ether is added and the mixture filtered. The precipitate is washed with diethyl ether. The filtrate is concentrated to an 238844 oil. The crude product is chromatographed on silica gel eluting with 4:1 hexane:ethyl acetate. The eluate is concentrated to yield (±)-cis-l,1-dimethylethyl 6-(cyanomethyl)-2,2-dimethyl-1,3-dioxane-4-acetate. 200 MHz NMR (CDC13) 5 1.36 (m, 1H)# 1.42 (s, 3H) , 1.49 (s, 9H), 1.50 (s, 3H), 1.79 (dt, 1H, J = 2.5 Hz, J = 12.1 Hz), 2.40 (dd, 1H, J = 6.2 Hz, J = 15.4 Hz), 2.5 - 2.7 (m, 1H), 2.55 (d, 2H, J = 6.1 Hz) 4.18 (m, 1H), 4.32 (m, 1H). 13C-NMR (CDC13, 50 MHz) 5 19.80, 25.16, 28.30, 29.94, 35.66, 42.56, 65.27, 65.87, 80.96, 99.57, 116.72, 169.83.

GCMS m/e 254, 199, 198, 154, 138, 59, 57, 43, 41.

FTIR (neat) 954.2, 987.6, 1152.3, 1201.1, 1257.7, 1316.9, 1368.3, 1383.7, 1728.4, 2253.1, 2942.4, 2983.5 cm-1.

Step B: Preparation of (±)-cis-l,1-dimethylethyl 6-(2-aminoethyl)-2,2-dimethyl-l,3-dioxane-4-acetate.

A solution of 6.75 g of (±)-cis-l,1-dimethyl ethyl 6-(cyanomethyl)-2,2-dimethyl-l,3-dioxane-4-acetate in 80 mL of methanol saturated with gaseous ammonia is treated with 0.7 g of Raney nickel #30 and hydrogen gas in a shaker at 50 pounds per square inch gage (psig) and 40°C. After 10 hours, thin layer chromatography indicates no starting nitrile present. The suspension is cooled, filtered through filter aid, and concentrated to an oil. This crude oil is purified by flash chromatography on silica gel with 30:20:1 (ethyl acetate:methanol-ammonium hydroxide) as eluent to give 5.48 g of (±)-cis-l,1-dimethylethyl o 238 8 4 4 6-(2-aminoethyl)-2,2-dimethyl-l,3-dioxane-4-acetate (98.2 area %) as a clear oil which hardens after time. 200 MHz NMR (CDC13) 6 1.0 - 1.2 (m, 1H), 1.22 (s, 3H), 1.31 (s, 12H), 1.35 - 1.45 (m, 1H), 1.77 (brS, 2H), 5 2.15 (dd, 1H, J = 15.1 Hz, J = 6.2 Hz), 2.29 (dd, 1H, J = 15.1 Hz, J = 7.0 Hz), 2.66 (t, 2H, J = 6.6 Hz), 3.82 (m, 1H), 4.12 (m, 1H). 13C-NMR (CDC13/ 50 MHz) 6 19.56, 27.92, 29.96, 36.43, 38.18, 39.65, 42.55, 66.03, 67.16, 80.19, 98.32, 10 169.80.

GC/MS m/e 258, 216, 215, 202, 200, 142, 113, 100, 99, 72, 57, 43.

FTIR (neat) 951.6, 1157.4, 1201.1, 1260.3, 1314.3, 1368.3, 1381.2, 1728.4, 2361.1, 2939.8, 2980.9 cm"1.

Step C: Preparation of Xgans-(±)-5-(4-fluorophenyl)-2-(1-methylethyl)-N,4-diphenyl-l-f2-(tetra-hydro-4-hydroxy-6-oxo-2H-pyran-2-yl )ethyl] -ig-pyrrole-3-carboxamide.

A solution of 0.79 g (2.89 mmol) of (±)-cis-20 1,1-dimethylethyl 6-(2-aminoethyl)-2,2-dimethyl-l,3-dioxane-4-acetate and 1.00 g (2.41 mmol) of (±)-4-fluoro-cr- [2-methyl-l-oxopropyl]-y-oxo-N, p-diphenylbenzenebutaneamide mixture of [R-(R*,R*)], [R-(R*,S*)], [S-(R*,R*)] and [S-(R*,S*)] isomers in 25 15 mL of heptane:toluene (9:1) is heated at reflux for 24 hours. The solution is cooled and poured into 100 mL of tetrahydrofuran and 50 mL of saturated ammonium chloride in water. The layers are separated and the organic layer washed with brine. To the 30 organic layer is added 5 mL of 10% hydrochloric acid solution and the solution is stirred for 15 hours. To 23 8 8 this solution is added 1.2 g of sodium hydroxide and the mixture is stirred for 30 hours. The reaction is stopped by adding 50 mL of water, 30 mL of hexane, and separating the layers. The aqueous layer is acidified 5 with dilute hydrochloric acid solution, stirred for three hours and extracted with 50 mL of ethyl acetate. A drop of concentrated hydrochloric acid is added to the ethyl acetate solution and the solution is allowed to stand 18 hours. The solution is concentrated in 10 vacuo and the concentrate is redissolved in 50 mL of ethyl acetate and treated with one drop of concentrated hydrochloric acid. The solution is stirred two hours, concentrated in vacuo, and dissolved in 10 mL of toluene. Trans - (±) - 5 - (4- f luorophenyl )-2-( 1 -methyl -15 ethyl )-N, 4-diphenyl-l- [2-(tetrahydro-4-hydroxy-6-oxo-2H-pyran-2-yl)ethyl ] -lH-pyrrole-3-carboxamide is isolated in two crops.

METHOD I Step A: Preparation of either (±)-(2a,4a,6a ) or 20 (±)-(2a,4g, 63)-l,1-dimethylethyl 6-(2-cyano- methyl) -2-phenyl-l, 3-dioxane-4-acetate.

Either (±)-(2a,4a,6a ) or (±)-(2a, 40,6p )-6-(cyanomethyl) -2-phenyl-l,3-dioxane-4-acetic acid (3.07 g, 11.75 mmol) is dissolved in 15 mL of dichloromethane, 25 cooled to 0°C and 0.1 g of 4-dimethylaminopyridine (DMAP) added, followed by t-butyl alcohol, followed by 2.91 g of dicyclohexylcarbodiimide (DCC). This solution is allowed to slowly warm to room temperature and stirred over a 16.5-hour period. Thin layer 30 chromatography (TLC) shows mainly product, and some slightly lower Rf by-products. The mixture is stirred one hour and 50 mL of dichloromethane is added, and stirring is continued for five hours. An additional 100 mL of diethyl ether is added and then filtered. £ 0 U U4 4 The precipitate is washed with diethyl ether. The filtrate is concentrated to an oil. The crude product is chromatographed on silica gel eluting with 4:1 hexane:ethyl acetate. The eluate is concentrated to 5 yield either (±)-(2a,4a,6a) or (±)-(2a,4p,60)-l,1-dimethylethyl 6-(2-cyanomethyl)-2-phenyl-l,3-dioxane-4-acetate.

GC/MS m/e 260, 244, 202, 138, 107, 105, 77, 57, 41.

Step B: Preparation of either (±)-(2a,4a,6a) or 10 (±)-(2a, 4(3, 60) 1,1-dimethylethyl 6— (2 — aminoethyl)-2-phenyl-l,3-dioxane-4-acetate. A solution of 1.72 g of either (±)-(2a,4a,6a) or (±)-(2a, 40, 6p)-l,1-dimethylethyl 6-(cyanomethyl)-2-phenyl-1,3-dioxane-4-acetate in 30 mL of methanol 15 saturated with gaseous ammonia is treated with 0.3 g of Raney nickel #30 and hydrogen gas in a shaker at 50 pounds per square inch gage (psig) and 40°C. After hours, thin layer chromatography indicates no starting nitrile present. The suspension is cooled, filtered through filter aid, and concentrated to an oil. This crude oil is purified by flash chromatography on silica gel with 30:20:1 (ethyl acetate:methanol: ammonium hydroxide) as eluent to give 1.56 g of either (±)-(2a,4a,6a) or 25 (±)-(2a,40,60)-l,1-dimethylethyl 6-(2-aminoethyl)-2-phenyl-l,3-dioxane-4-acetate (98.2 area %) as a clear 011 which hardens after time. 200 MHz NMR (CDC13) 6 1.2 - 1.9 (m, 4H) 1.44 (s, 9H), 2.03, (br.s, 2H), 2.42 (dd, 1H, J = 15.3 Hz, J = 6.3 30 Hz), 2.63 (dd, 1H, J = 15.3 Hz, J = 7.0 Hz), 2.89 (t, 2H, J = 6.8 Hz), 3.97 (m, 1H), 4.26 (m, 1H), 5.56 (s, 1H), 7.3 - 7.4 (m, 3H), 7.4 - 7.5 (m, 2H). 238S 13C NMR (CDCI3, 50 MHz) 6 28.07, 36.57, 38.23, 39.25, 42.17, 73.47, 74.87, 80.60, 100.36, 125.82, 127.88, 128.34, 138.45, 169.73.

GC/MS, m/e 321, 320, 248, 215, 174, 142, 105, 57.

FTIR (film) 699.6, 756.2, 1026.2, 1116.2, 1149.7, 1368.3, 1394.0, 1718.1, 1733.5, 2872.9, 2932.1 cm-1.

Step C: Preparation of Trass-(±)-5-(4-fluorophenyl)-2 - (1-methylethyl) -N, 4-diphenyl-l - f2 - (tetra-hydro-4-hydroxy-6-oxo-2g-pyran-2-yl) ethyl] -10 lg-pyrrole-3-carboxamide.

In a process analogous to Method H by substituting (±)-(2a,4a,6a) or (±)-(2«,4p,6p) 1,1-dimethylethyl-6- (2-aminoethyl) -2-phenyl-l, 3 -dioxane-4-acetate for (±)-cis-l,1-dimethylethyl 6-(2-amino-15 ethyl)-2,2-dimethyl-l,3-dioxane-4-acetate one obtains the title compound.

METHOD J Step A: Preparation of (t)-cis-l,1-dimethylethyl 4-(cyanomethyl) -1,5-dioxaspiro [5.5] undecane-20 2-acetate. (±)-cis-4-(cyanomethyl)-1,5-dioxaspiro [5.5]-undecane-2-acetic acid 3.32 g (13.12 mmol), is dissolved in 15 mL of dichloromethane, cooled to 0°C and 0.1 g of 4-dimethylaminopyridine (DMAP) added, 25 followed by t-butyl alcohol, and followed by 3.25 g of dicyclohexylcarbodiimide (DCC). This solution is stirred and allowed to slowly warm to room temperature over a 16.5-hour period. TLC shows mainly product, and some slightly lower Rf by-product. The mixture is 30 stirred one hour and 50 mL of dichloromethane is added and stirring continued four hours. One hundred mL of o 238844 diethyl ether is added and then filtered. The filtrate is concentrated at reduced pressure. This crude concentrate is chromatographed on silica gel and eluted with 4:1 hexane-.ethyl acetate to yield 5 (±)-cis-l< 1-dimethylethyl 4-(cyanomethyl )-l, 5-dioxaspiro [5.5]undecane-2-acetate. 200 MHz NMR (CDCl3) 6 1.1 - 2.0 (m, 12H) 1.43 (s, 9H), 2.36 (m, 2H), 2.48 (m, 2H), 4.1 - 4.4 (m, 2H). 13C-NMR (CDC13, 50 MHz) 22.37, 22.45, 25.08, 28.15, 10 28.55, 35.80, 38.57, 42.59, 64.31, 64.92, 80.76, 99.56, 116.65, 169.82.

GC/MS m/e 309 (m+), 266, 224, 210, 138, 120, 99, 57, 55.

FTIR (KBr) 964.5, 1149.7, 1157.4, 1332.3, 1368.3, 15 1712.9, 2939.8 cm-1.

Step B: Preparation of (±)-cis-l, 1-dimethylethyl 4- (2-aminoethyl)-1,5-dioxaspiro[5.5]-undecane-2-acetate.

A solution of 1.19 g of (±)-cis-l, 1-dimethyl-20 ethyl 4-(cyanomethyl)-l, 5-dioxaspiro [5.5]undecane-2-acetate in 30 mL of methanol saturated with gaseous , ammonia is treated with 0.3 g of Raney nickel #30 and hydrogen gas in a shaker at 50 pounds per square inch gage (psig) and 40°C. After 22 hours, thin layer 25 chromatography indicates no starting nitrile present. The suspension is cooled, filtered through filter aid, and concentrated to an oil. This crude oil is purified by silica gel flash chromatography (30:20:1; ethyl acetate:methanol:ammonium hydroxide) to give 30 1.18 g of (±)-cis-l, 1-dimethylethyl 4-(2-aminoethyl)- 238844 O 1,5-dioxaspiro[5.5]undecane-2-acetate as a clear oil which solidifies upon standing.

O 200 mz NMR (CDC13) 6 1.2 - 2.0 (m, 12H), 1.43 (s, 9H), 2.34, (m, 2H), 2.50 (br.s, 2H), 2.84 (t, 2H, J = 5 6.7 Hz), 3.99 (m, 1H), 4.28 (m, 1H).

GC/MS, m/e 313, 270, 214, 185, 144, 142, 99. o FTIR (film) 961.9, 1098.2, 1154.8, 1368.3, 1725.8, 2934.6 cm"1.

Step C: Preparation of Trans-(±)-5-(4-fluorophenyl)-2-(1-methylethyl)-N,4-diphenyl-l-[2-(tetra-hydro-4-hydroxy-6-oxo-2g-pyran-2-yl)ethyl1-lg-pyrrole-3-carboxamide.

In a process analogous to Method H by substituting (±)-cis-1,1-dimethylethyl 4-(2-aminoethyl )-1,5-dioxaspiro[5.5]undecane-2-acetate for (±)-cis-1,1-dimethylethyl 6-(2-aminoethyl)-2,2-dimethyl-1', 3-dioxane-4-acetate one obtains the title compound.

EXAMPLE 3 (2R-Xr3as-5- (4-f luorophenyl) -2- (1-methylethyl) -N, 4-diphenyl-1- F2- (tetrahydro-4-hydroxy-6-oxo-2g-pyran-2-yl)ethyl]-lg-pyrrole-3-carboxamide.

METHOD A Step A: Preparation of (R)-l, 1-dimethylethyl 6-cvano-5-fr (1/ 1-dimethylethyl) dimethyls ilylloxy] -3-25 oxohexanoate.

(R) -4-cyano-3- [ [ (1,1-dimethylethyl)dimethylsilyl ] -oxy]butanoic acid, 32 g (0.132 mol), is dissolved in 300 mL of tetrahydrofuran. The solution is cooled to O 238844 -20°C and carbonyldiimidazole, 27 g (0.165 mol), is added. The solution is stirred and allowed to warm to 25°C over two hours. The solution is added to a slurry of potassium 1,1-dimethylethyl malonate (half 5 ester, half salt), 60 g (0.3 mol), anhydrous magnesium chloride, 27.2 g (0.246 mol), diisopropylethylamine, 53 raL (0.3 mol) in 700 mL of dry acetonitrile. The mixture is stirred at 5°C for 18 hours and at 15°C for 108 hours. The mixture is poured into a mixture of 10 1 L of IN hydrochloric acid and 1 L of ethyl acetate and the resulting two-phase system is stirred for 15 minutes. The layers are separated. The organic layer is washed with 500 mL of saturated salt solution and concentrated to yield an oil. The oil consists of 15 (R)-l,1-dimethylethyl 6-cyano-5-[[(l, 1-dimethylethyl)-dimethylsilyl]oxy]-3-oxohexanoate and some 1,1-dimethylethyl malonate (half ester, half acid) that is used directly in Step B. The oil has acceptable NMR spectra after subtracting the recovered 20 malonate spectra.

Step B: Preparation of (R)-l,1-dimethylethyl 6-cyano-5-hydroxy-3-oxo-hexanoate.

A solution of crude (R)-l, 1-dimethylethyl 6-cyano-5- [ [ (1,1-dimethylethyl)dimethylsilyljoxy] -3-25 oxohexanoate, 43 g (0.126 mol) in 350 mL of tetrahydrofuran is treated with 213 mL of tetrabutylammonium fluoride solution (1.0'M in hexane). The resulting mixture is stirred for five hours, at 25°C. The mixture is treated with 500 mL of water, 300 mL of 30 diethyl ether is added, and the layers separated. The organic layer is dried (magnesium sulfate) and then filtered through a plug of silica gel with the aid of anhydrous diethyl ether. The solvent is removed under 238 8 4 a vacuum to obtain 21 g of crude (R)-l,1-dimethylethyl 6-cyano-5-hydroxy-3-oxohexanoate with acceptable NMR, MS and IR spectra. 200 MHz NMR (CDC13) 6 1.48 (s, 9H), 2.62 (m, 2H), 2.89 5 (d, 2H, J = 6.1), 3.43 (s, 2H), 4.41 (pentet, 2H, J = 6.1 Hz) 13C-NMR (CDCI3, 50 mHz) 5 25.05, 27.86, 48.03, 50.81, 63.39, 82.43, 117.03, 165.84, 202.03.

MS (Chemical ionization) m/e 228, 200, 172, 154.

FTIR (KBr) 1144.5, 1327.2, 1370.9, 1715.5, 1733.5, 2253.1, 2934.6, 2980.9, 3459.3 cm-1.

Step C: Preparation of FR-(R*,R*)1-1,1-dimethylethyl 6-cyano-3,5-dihydroxyhexanoate.

Crude (R)-l,1-dimethylethyl 6-cyano-5-hydroxy-3-15 oxohexanoate, 21 g (0.0924 mol), is dissolved in 940 mL of tetrahydrofuran and 190 mL of methanol under a nitrogen atmosphere. This solution is cooled to -85 °C and 95 mL of a 15% solution of me thoxydi ethyl-borane in tetrahydrofuran is added. The reaction is 20 cooled to -97°C and 6.5 g (0.172 mol) of sodium borohydride is added in 0.5 g portions over 1.5 hours. The reaction is maintained between -93°C and -97 °C for 13 hours and allowed to warm to room temperature and stand for 60 hours under a nitrogen atmosphere. The 25 reaction is quenched by the addition of 25 mL (0.395 mol) acetic acid and concentrated by vacuum distillation to an oil. The residue is dissolved with 500 mL methanol, concentrated by vacuum distillation, redissolved with 500 mL methanol and reconcentrated by 30 vacuum distillation to give a dark brown oil. This oil is taken up in 500 mL of ethyl acetate and ^ - lrJ— 238 8 filtered through a plug of silica gel with the aid of 250 mL of ethyl acetate. The solution is evaporated to give 15 g of crude [R-(R*,R*) ]-l, 1-dimethylethyl 6-cyano-3,5-dihydroxyhexanoate which is used without 5 further purification.

Step D: Preparation of (4R-cis)-l,1-dimethylethyl 6-cyanomethyl-2, 2-dimethyl-l, 3-dioxane-4-acetate.

Crude [R-(R*,R*)]-1,1-dimethylethyl 6-cyano-3,5-• 10 dihydroxyhexanoate, 15 g (51 mol), is dissolved in 150 mL of 2,2-dimethoxypropane, camphorsulfonic acid is added, and the solution is stirred for 18 hours at room temperature. Concentration and flash chromatography after concentration in vacuo provides 11.8 g of 15 (4R-cis)-1,1-dimethylethyl 6-cyanomethyl-2,2-dimethyl-l,3-dioxane-4-acetate as an off-white solid, mp 64.7-68°C with acceptable IR, NMR, C-NMR and analysis. 200 MHz NMR (CDC13 ) 6 1.36 (m, 1H), 1.42 (s, 3H), 1.49 20 (s, 9H), 1.50 (s, 3H), 1.79 (dt, 1H, J = 2.5 Hz, J = 12.1 Hz), 2.40 (dd, 1H, J = 6.2 Hz, J = 15.4 Hz), 2.5 - 2.7 (m, 1H), 2.55 (d, 2H, J = 6.1 Hz), 4.18 (m, 1H), 4.32 (m, 1H). 13C-NMR (CDCI3, 50 MHz) 5 19.74, 25.09, 28.24, 29.88, 25 35.58, 42.50, 65.20, 65.81, 80.87, 99.48, 116.68, 169.75.

GC/MS m/e 254, 198, 154, 138, 120, 59, 57, 43, 41.

FTIR (KBr) 941.4, 1116.2, 1154.8, 1188.3, 1257.7, 1293.7, 1309.1, 1368.3, 1725.8, 2361.1, 2983.5, 30 2996.4 cm-1. 23 8 8 4 Step E: Preparation of (4R-cis)-l,l-dimethvlethyl 6-(2-aminoethyl)-2,2-dimethyl-l,3-dioxane-4-acetate.

A solution of (4R-cis)-l,1-dimethylethyl 6-cyano-5 methyl-2,2-dimethyl-l,3-dioxane-4-acetate, 5.63 g (0.048 mol), in 100 mL of methanol saturated with gaseous ammonia is treated with 0.5 g of Raney nickel #30 and hydrogen gas in a shaker at 50 psi and 40°C.

After 16 hours, thin layer chromatography indicates no 10 starting nitrile present. The suspension is cooled, filtered through filter aid, and concentrated to an oil. This crude oil is purified by flash chromatography on silica gel with 30:20:1 (ethyl acetate:methanol:ammonium hydroxide) as eluent to give 4.93 g of of (4R-cis)-l,1-15 dimethylethyl 6-(2-aminoethyl)-2,2-dimethyl-l,3- dioxane-4-acetate (98.2 area %) as a clear oil with acceptable IR, NMR, C-NMR and MS spectra. 200 MHz NMR (CDC13) 1.0 - 1.2 (m, 1H), 1.22 (s, 3H), 1.31 (s, 12 H), 1.35 - 1.45 (m, 3H), 2.15 (dd, 1H, J = 20 15.1 Hz, J = 6.2 Hz), 2.29 (dd, 1H, J = 15.1 Hz, J = 7.0 Hz), 2.66 (t, 2H, J = 6.6 Hz), 3.82 (m, 1H), 4.12 (m, 1H). 13C-NMR (CDC13, 50 MHz) 6 19.60, 27.96, 30.00, 36.50, 38.25, 39.79, 42.61," 66.08, 67.18, 80.21, 98.35, - 25 169.82.

GC/MS m/e 202, 200, 173, 158, 142, 140, 114, 113, 100, 99, 97, 72, 57.

FTIR (neat) 951.6, 1159.9, 1201.1, 1260.3, 1314.3, 1368.3, 1381.2, 1731.0, 2870.3, 2939.8, 2980.9, 3382.2 cm"1.

Step F: Preparation of (4R-cis)-l,1-dimethylethyl 6-[2f2-(4-fluorophenyl)-5-(1-methylethyl)-3-phenyl-4- f (phenylamino)carbonyl 1 -lg-pyrrol-1-yl 1 ethyl 1 -2, 2-dimethyl-l, 3-dioxane-4-acetate.

A solution of (4R-cis)-l,1-dimethylethyl 6-(2- aminoethyl)-2,2-dimethyl-l,3-dioxane-4-acetate, 1.36 g (4.97 mol), and (±)-4-fluoro-a-[2-methyl-l-oxopropyl]-y-oxo-N,p-diphenylbenzenebutaneamide mixture of [R-(R*,R*)], [R-(R*,S*)], [S-(R*,R*)] and [S-R*,S*)] 10 isomers, 1.60 g (3.83 mol), in 50 mL of heptane:toluene (9:1) is heated at reflux for 24 hours. The solution is cooled slightly and 15 mL of 2-propanol added. The mixture is allowed to cool to 25°C and filtered to give 1.86 g of (4R-cis)-l,1-15 dimethylethyl 6-[2[2-(4-fluorophenyl)-5-(1-methylethyl ) -3 -phenyl-4- [ (phenylamino)carbonyl]-lH-pyrrol-1-yl]ethyl]-2,2-dimethyl-l,3-dioxane-4-acetate as a yellow solid with acceptable PNMR & C-NMR spectra. 1H-NMR (CDC13, 200 MHz) 5 1 - 1.7 (m, 5H), 1.30 (s, 20 3H), 1.36 (s, 3H), 1.43 (s, 9H), 1.53 (d, 6H, J = 7.1 Hz), 2.23 (dd, 1H, J = 15.3 Hz, J = 6.3 Hz), 2.39 (dd, 1H, J = 15.3 Hz, J = 6.3 Hz), 3.5 - 3.9 (m, 3H), 4.0 - 4.2 (m, 2H), 6.8 - 7.3 (m, 14H). 13C-NMR (CDCI3, 50 MHz) 6 19.69, 21.60, 21.74, 26.12, 25 27.04, 28.12, 29.95, 36.05, 38.10, 40.89, 42.54, 65.92, 66.46, 80.59, 98.61, 115.00, 115.34, 115.42, 119.52, 121.78, 123.36, 126.44, 128.21, 128.31, 128.52, 128.75, 130.43, 133.01, 133.17, 134.69, 138.38, 141.47, 159.72, 164.64, 169.96. 23 8 8 4 4 Step G: Preparation of (2R-traas)-5-(4-fluorophenyl)-2-(1-methylethyl)-N,4-diphenyl-l-[2-(tetra-hydro-4-hydroxy-6-oxo-28-pyran-2-yl)ethyl] -lg-pyrrole-3-carboxamide. (4R-cis)-1,1-dimethylethyl 6-[2[2-(4-fluoro phenyl )-5-(1-methylethyl)-3-phenyl-4-[(phenylamino)-carbonyl]-lH-pyrrol-l-yl]ethyl]-2,2-dimethyl-1,3-dioxane-4-acetate, 4.37 g (6.68 mol), is dissolved in 200 mL of tetrahydrofuran and 15 mL of 10% hydrochloric acid solution is added, and the solution is stirred for 15 hours. To this solution- is added sodium hydroxide (3.6 g) and the mixture is stirred for 30 hours. The reaction is stopped by adding 150 mL of water, 90 mL of hexane, and separating the 15 layers. The aqueous layer is acidified with dilute hydrochloric acid solution, stirred for three hours and extracted with 150 mL of ethyl acetate. A drop of concentrated hydrochloric acid is added to the ethyl acetate solution and the solution is allowed to stand 20 18 hours. The solution is concentrated in vacuo and the concentrate is redissolved in 50 mL of ethyl acetate and treated with one drop of concentrated hydrochloric acid. The solution is stirred two hours, concentrated in vacuo, and dissolved in 3.0 mL of 25 toluene. (2R-trans)-5-(4-fluorophenyl)-2-(l-methyl-ethyl) -N, 4-diphenyl-l- [2- (tetrahydro-4-hydroxy-6-oxo-2H-pyran-2-yl)ethyl]-lH-pyrrole-3-carboxamide (3.01 g) is isolated in two crops.

METHOD B A solution of (4R-cis)-l,1-dimethylethyl 6-(2-aminoethyl)-2,2-dimethyl-l,3-dioxane-4-acetate, 2.56 g (9.36 mol), and (±)-4-fluoro-ct-[2-methyl-l-oxopropyl] -y-oxo-N,p-diphenylbenzenebutaneamide mixture of [R-(R*,R*)], [R-(R*,S*)], [S-(R*,R*)] and 35 [S-(R*, S*) ] isomers, 3.00 g (7.20 mol), in 60 mL of 0 f 0 ^ heptane:toluene (9:1) is heated at reflux for 24 hours. The solution is cooled and poured into 300 mL of tetrahydrofuran and 150 mL of saturated ammonium chloride in water. The layers are separated 5 and the organic layer is added to 15 mL of 10% hydrochloric acid solution and the solution is stirred for 15 hours. To this solution is added sodium hydroxide (3.6 g) and the mixture is stirred for 30 hours. The reaction is stopped by adding 150 mL of 10 water, 90 mL of hexane, and separating the layers. The aqueous layer is acidified with dilute hydrochloric acid solution, stirred for three hours and extracted with 150 mL of ethyl acetate. A drop of concentrated hydrochloric acid is added to the ethyl 15 acetate solution and the solution is allowed to stand 18 hours. The solution is concentrated in vacuo and the concentrate is redissolved in 50 mL of ethyl acetate and treated with one drop of concentrated hydrochloric acid. The solution is stirred 20 two hours, concentrated in vacuo, and dissolved in 3.0 mL of toluene. (2R-trans)-5-(4-fluorophenyl)-2-(1-methylethyl)-N,4-diphenyl-l-[2-(tetrahydro-4-hydroxy-6-oxo-2H-pyran-2-yl)-ethyl]-lH-pyrrole-3-carboxamide (2.92 g) is isolated in two crops.

PREPARATION OF STARTING MATERIALS A EXAMPLE A 1-(4-Fluorophenyl)1,4-hexanedione.

A mixture of 36.61 g (295 mmol) of 4-fluorobenz-aldehyde, 25 g (297.2 mmol) of ethyl vinyl ketone, 30 29 mL (206.9 mmol) of triethylamine and 11.94 g (44.25 mmol) of 3-benzyl-5-(2-hydroxyethyl)-4-methyl-thiazolium chloride is stirred and heated at 70°C for six hours. The cooled solution is diluted with o o 23884 4 2 liters of diethyl ether and washed with 2 x 300 mL of water, 2 x 100 mL of 2M hydrochloric acid, 100 mL of water, 200 mL of a saturated solution of sodium O bicarbonate and brine. The organic layer is separated, dried (magnesium sulfate), filtered, and concentrated to provide 55 g of l-(4-fluorophenyl)-1,4-hexanedione after recrystallization from methanol; mp 56-57°C.

EXAMPLE B 4-Methyl-3-oxo-N-phenylpentan amide A three-necked, 12-L round-bottom flask equipped with a mechanical stirrer, a thermometer and set up for distillation is charged with 2.6 L of toluene, 1.73 kg (12 mol) of methyl 4-methyl-3-oxopentanoate 15 and 72 g (1.18 mol) of ethylene diamine. The mixture is heated to 80°C and charged with 0.49 kg of aniline. The mixture is brought to reflux and distillation started. After 40 minutes a further 0.245 kg of aniline is charged and at 40 minute intervals a 20 further two portions of aniline (0.245 and 0.25 kg) are charged. Distillation is continued for a further one to five hours until a total of 985 mL of solvent is removed. The solution is stirred at room temperature for 16 hours and a further 550 mL of 25 solvent is removed by vacuum distillation (using a approximately 85 mm Hg). The mixture is cooled and 2 L of water is charged to provide an oil. The mixture is warmed to 40°C and a further 1.0 L of water is charged. Seven hundred milliliters of 30 toluene-water mixture is removed by vacuum distillation (approximately 20 mm Hg). Two liters of water is charged and the mixture is allowed to stand for 10 days. The product is isolated by filtration and washed with three portions of hexane. Drying in vacuo gives 1.7 kg of 4-methyl-3-oxo-N-phenylpentan-amide as a hydrate; mp 46.5-58.8°C.

HPLC: 98.8% - retention time 3.56 min. 65/35 acetonitrile/water on a dry basis.

VPC: 87.6% - retention time 12.43 min. also 10.8% aniline (decomposition). 238844

Claims (4)

WHAT WE CLAIM IS:
1. A compound of Formula XVI XVI wherein R^q and are independently alkyl of one to eight carbon atoms or R10 and Rn together are -ch2-CH- or -CH2-CH2-CH2-. CH3
2. 2. A compound according to claim 1 wherein R^q and R^ are methyl or ethyl or R^q and R^ together are -CE2CH-. CH3
3. 3. A compound as claimed in claim 1 and as specifically identified in this specification.
4. 4. A process for producing a compound as claimed in claim 1, substantially as described in this specification. \ 15 AUG 19913 W J 4b WARNER-LAMBERT COMPANY ATTORNEYS SON & CAREY