NZ229331A

NZ229331A - Triene-substituted chromene derivatives and their use as pharmaceutical compositions

Info

Publication number: NZ229331A
Application number: NZ22933189A
Authority: NZ
Inventors: Jean-Daniel Brion; Baut Guillaume Le; Patrick Ducrey; Sylvie Piessard-Robert; Claude Cudennec; Genevieve Seurre
Original assignee: Adir
Priority date: 1989-05-29
Filing date: 1989-05-29
Publication date: 1990-12-21

Description

<div class="application article clearfix" id="description"> New Zealand Paient Spedficaiion for Paient Number £29331 r NO DRAWINGS ^ ^ 9 3 $ 1 Priority Date{$): Complete Specification Filed: Class: £r??71 Q2s\ \ /.£>!?>.') . .£\fc>A .l£3. \ ./.£>£>. Publication Date: ... ?.DEC 1990 P.O. Journal, No: .... No.: Date: NEW ZEALAND PATENTS ACT, 1953 -■ a1 •? 29 MAY1989"! ■-v?« .<?.%/ COMPLETE SPECIFICATION #fe_ change addrefJ ?[^w, —Su^-Se-i-ne-r-France, NEW TRIENE DERIVATIVES HAVING A CHROMENE STRUCTURE, PROCESSES FOR PREPARING THEM AND PHARMACEUTICAL COMPOSITIONS CONTAINING THEM HU/ ET COMPAGNIE, a French company of^-22-^€-6awrerr_F-9220-l~Nei>i-14y y hereby declare the invention, for which -i"/ we pray that a patent maybe granted to p«eVus, and the method by which it is to be performed, to be particularly described in and by the following statement: - - 1 - (followed by page la) 22 9 3 3 - la- The present invention relates to new derivatives endowed with anticancer properties belonging to the retinoid family, to a process for preparing them and to pharmaceutical compositions containing them. 5 Many substances of the retinoid family which are renowned for their anticancer properties are known. Patents US 4,054,589, US 4,106,681, US 4,137,246, US 4,165,103, US 4,169,100, US 4,171,318, US 4,224,244, BE 861,982, FR 2,556,348 and EP 111,124 describe 10 derivatives of the retinoid family in which the tetraene chain is attached to a monocyclic structure based on cyclohexene or benzene. More especially, US Patent 4,105,681 describes etretinate, used in therapy, and whose anticancer pro-15 perties are known at the present time. US Patent 4,321,209 as well as Priority Documents DT 2,542,600 and DT 3,542,601 describe derivatives in which the triene chain is attached to an aromatic monocyclic structure. 20 The needs of therapy demand the constant development of new anticancer agents, with the dual objective of obtaining molecules which are more active but, at the same time, less toxic. The derivatives of the present invention 25 possess a novel structure consisting of a triene chain attached to a bicyclic structure of the chromene type, which is to be found in many natural substances, the structure optionally being substituted on the aromatic ring. The novelty of the structure of the compounds of 30 the present invention makes it possible to obtain especially advantageous pharmacological properties, since the latter prove, in the tests studied, to be superior to that of etretinate. In addition, their toxicity is especially low, since it was found that 35 these derivatives caused no excess-vitamin A toxicity, in contrast to etretinate. More especially, the subject of the present invention is products of general formula (I): 22 933 - 2 - 10 (I) in which: R^, R2, R3 and R4, which may be identical or different, denote a hydrogen atom, a halogen atom 15 or a lower alkyl, lower alkenyl, lower alkyloxy or lower alkenyloxy group, optionally substituted with one or more halogen atoms, Rg denotes a carboxyl, (lower alkyloxy)carbonyl, (lower alkenyloxy)carbonyl or (lower alkynyloxy)-20 carbonyl, their isomers, stereoisomers and diastereoisomers, and also their addition salts with a pharmaceu-tically acceptable base. Among bases capable of salifying the compounds 25 of formula (I) in which Rg denotes a carboxyl group, there may be mentioned, by way of example, sodium hydroxide, potassium hydroxide, calcium hydroxide or aluminum hydroxide, alkali metal or alkaline-earth metal carbonates, or organic bases such as triethyl-30 amine, benzylamine, diethanolamine, tert-butylamine, dicyclohexylamine, arginine, and the like. Lower alkyl, lower alkenyl, lower alkyloxy, lower alkenyloxy and lower alkynyloxy radicals are understood to mean linear or branched groups comprising 35 between 1 and 6 carbon atoms. The present invention also encompasses a process for preparing the derivatives of formula (I), wherein a derivative of formula (II): 229331 (ii) in which R^, R2/ R3 and R4 have the same meaning as in 10 the formula (I), is condensed by heating under reflux of the solvent with 3-oxo-l-butene of formula (III): 15 (HI) 20 in the presence of an alkaline agent preferably chosen from alkali metal and alkaline-earth metal carbonates, or organic bases such as, for example, triethylamine or pyridine, or a mixture consisting of an alkali metal carbonate or alkaline-earth metal carbonate and an 25 organic base, in a polar solvent preferably chosen from ketonic solvents such as 2-butanone or amidic solvents such as dimethylformamide, to lead, after cooling and, where appropriate, evaporation of the reaction medium, taking up with water to which an 30 alkaline agent such as sodium hydroxide has been added where appropriate, extraction with a suitable organic solvent such as diethyl ether, chloroform or methylene chloride, washing of the organic phase, evaporation of the solvent and purification of the residue by 35 crystallization or distillation or chromatography on a silica or alumina column, to a derivative of formula (IV): 22 9 3 3 1 - 4 - (IV) in which R^, R2/ R3 and R^ have the same meaning as in formula (I), which derivative is subjected 10 * either to catalytic hydrogenation, after dissolution in a suitable organic solvent preferably chosen from lower aliphatic alcohols, preferably in the presence of an alkaline agent such as sodium hydroxide, the hydrogenation preferably being carried out with an 15 alkali metal mixed hydride such as sodium borohydride, to lead, after extraction with a suitable organic solvent such as diethyl ether, dichloromethane or chloroform, preceded where appropriate by evaporation of the 20 reaction medium, washing, then evaporation of the organic phase and purification, to a derivative of formula (V): 25 R, ch3 (V) 30 35 in which R^, R2, R3 and R4 have the same meaning as above, which derivative is subjected to the action of a tri-phenylphosphonium salt in a solvent preferably chosen from lower aliphatic alcohols, at room temperature and with stirring, to lead, after evaporation of the solvent and purification by chromatography on silica gel or by crystallization in a solvent or a mixture of suitable solvents, - 5 - 22 933 to a derivative of formula (VI): 20 25 R' CH3 R4 (VI) 10 in which A" denotes the anion of a hydracid and R^, R2, R3 and R^ have the same meaning as above, which is subjected, in a suitable solvent, preferably under an inert atmosphere and in the presence of n-butyllithium in hexane, to the action of a stereochemi-15 cally blocked dienic aldehyde in the form of a tricar-bonyliron complex of formula (VII) according to the reaction described by WITTIG (Ber 1954, 82, 1318 and Ber 1955, M/ 1654) : CH, / W OHC-+ v—R, (VII) xFe' 8 ' I X CO ' CO CO in which Rg has the same meaning as in the formula (I), at a temperature preferably of between -5°C and -30°C, and preferably of between -15°C and -20°C, to lead, after extraction with a suitable solvent, washing and 30 evaporation of the organic phase, to an intermediate complex which, after purification by chromatography, is subjected, under reflux of the organic solvent chosen for this stage, to the action of trimethylamines with N-oxide, to lead, after cooling, extraction with a 35 suitable organic solvent, washing and evaporation of the organic phase and column chromatography, to a compound of formula (I), * or to the action of ethyl bromoacetate in the presence of zinc, followed by a hydrolysis of the 229331 - 6 - 10 reaction medium at the required moment and extraction with a suitable organic solvent preferably chosen from ethyl acetate, methylene chloride, chloroform and diethyl ether, and then to the action of phosphorus oxychloride in a suitable organic solvent, evaporation of the reaction medium and a further extraction with a solvent preferably chosen from ethyl acetate, methylene chloride, chloroform and diethyl ether, to lead, where appropriate after chromatography on silica gel, to a derivative of formula (VIII) 15 COOC2HS (VIII) in which R^, R2, R3, and R4 have the same meaning as in the formula (I), 20 which is subjected at a low temperature preferably of between -50 and 0°C, and preferably between -25 and -5°C, in a solvent preferably chosen from diethyl ether, diisopropyl ether and tetrahydrofuran, to the action of lithium aluminum hydride, to lead to a derivative of 25 formula (IX): 30 35 CHjOH in which R^, R2, R3 and R4 have the same meaning as in the formula (I), which is subjected to oxidation with manganese dioxide to lead, after chromatography on silica gel and crystallization where appropriate, to a derivative of formula (X): 22 9 3 3 1 - 7 - 15 CHO (X) in which R^, R2/ R3 and R4 have the same meaning as in the formula (I), 10 which is subjected to the action of a derivative of formula (XI) according to the WADSWORTH-EMMONS reaction (J. Am. Chem. Soc. 1961, 83, 1733): (ZO)2_ P (XI) 20 in which Rg has the same meaning as in the formula (I) and Z is a lower alkyl group, at room temperature, to lead, after extraction with a suitable organic solvent, evaporation and purification by chromatography on a silica column, to a compound of 25 formula (I), which can, if so desired, - either, when Rg denotes a carboxyl group, be salified with a pharmaceutically acceptable base, - or be separated into its stereoisomers by a 30 technique of crystallization or chromatography and then, if so desired, when Rg denotes a carboxyl group, be salified with a pharmaceutically acceptable base. The compounds of formula (I) possess very advantageous pharmacological properties. They strongly 35 inhibit the growth of L 1210 line in mice, have an activity on the differentiation of HL 60 line cells and, an especially advantageous factor for their use in therapy, do not appear to cause excess-vitamin A toxicity, which is induced by etretinate and the 22 9 3 3 1 symptoms of which are rapid weight loss, alopecia and bone weakening. The compounds according to the present invention hence find a use in therapy as antitumor agents, for the treatment or prophylaxis of benign or malignant neoplasms, as well as in the traditional indications for retinoids, such as skin disorders (acne, psoriasis), as well as degenerative disorders and/or inflammation of the mucosae. The subject of the present invention is also the pharmaceutical compositions containing the products of the formula (I), or one of their addition salts with a pharmaceutically acceptable base when Rg denotes a carboxyl group, alone or in combination with one or more pharmaceutically acceptable, non-toxic, inert vehicles or excipients. Among the pharmaceutical compositions according to the invention, there may be mentioned, more especially, those which are suitable for oral, parenteral, nasal, rectal, perlingual, ocular or respiratory administration, and in particular, tablets, simple or sugar-coated, sublingual tablets, sachets, packs, hard gelatin capsules, preparations to be dissolved under the tongue, bars, suppositories, creams, ointments, skin gels, injectable preparations or preparations to be swallowed, aerosols, eye or nose drops, and the like. The appropriate dosage varies according to the patient's age and weight, the administration route and the nature of the therapeutic indication and of any associated treatments, and ranges between 0.1 and 200 mg per day. The examples which follow illustrate the invention but in no way limit the latter. The preparations described below do not enable products of the invention to be obtained. They nevertheless enable intermediates to be obtained which are useful in the synthesis of the products of the invention. - 9 - 229331 PREPARATION Is TRICARBONYL{1—4-n-[(IE,3E)-1- ETHOXYCARBONYL-2-METHYL-5-OXO-1,3-PENTADIENYL]}IRON STAGE A: ETHYL 4,4-DIMETHOXY-3-METHYL-2-BUTENOATE A mixture of 50 mmol of 1,1-dimethoxyacetone and 60 mmol of ethyl 2-(diethoxyphosphoryl)acetate is added in the course of 1 hour to a suspension of 125 mmol of potassium carbonate in 10 ml of water with vigorous stirring and with temperature maintained at between 20 and 30°C. When the addition is complete, stirring is continued for 24 hours at room temperature and the insoluble matter is then removed by filtration and washed with diethyl ether. The organic phase is separated and washed with saturated sodium chloride . solution to neutrality. After drying and evaporation of the solvent, the product is purified by distillation under vacuum, which yields an (E + Z) isomer mixture in the form of an oil. STAGE B: ETHYL (E)-3-METHYL-4-OXO-2-BUTENOATE 50 mmol of ethyl (E + Z)-4,4-dimethoxy-3-methyl-2-butenoate are placed in a buffered acetic medium (3 g of sodium acetate, 1.5 ml of water, 30 ml of acetic acid). The reaction medium is heated to 100°C for 5 hours and the solvents are then removed under vacuum. After the residue is taken up with water and 100 ml of diethyl ether are added, the organic phase is separated and washed with 100 ml of saturated sodium chloride solution. After drying and evaporation of the solvents, purification is performed by distillation under vacuum, and yields thei E stereoisomer. Yield: 85% B.d^ = 44-46°C STAGE Cj ETHYL (2E,4E)-3-METHYL-6-0X0-2,4- HEXADIENOATES A solution of 50 mmol of ethyl (E)-3-methyl-4-oxo-2-butenoate in 25 ml of benzene is added in the course of 10 minutes, with vigorous stirring and under a nitrogen atmosphere, to a benzene suspension (100 ml) of 50 mmol of formylmethylenetriphenylphosphorane, the - 10 - 22 9 3 3 1 temperature being maintained at about 0°C. After return to room temperature, the reaction medium is heated under reflux for 6 hours. After being cooled, the medium is poured into a water/ethyl ether mixture and 5 the organic phase is then separated and dried. Evapora tion of the solvents is followed by chromatography on a column of silica gel (SiC^: 70-230 mesh; eluent: CH2CI2), which yields an oil (mixture of isomers) from which the (E,E) isomer is separated by fractional 10 crystallization. Yield: 65%. STAGE D: TRICARBONYL{1—4-n-[(IE,3E)-1- ETHOXYCARBONYL-2-METHYL-5-0X0-1,3-PENTADIENYL]>IRON 15 A solution of 10 mmol of ethyl (2E,4E)-3- methyl-6-oxo-2,4-hexadienoate in 75 ml of toluene is outgassed with a stream of nitrogen for 30 minutes before the addition of 34 ml (21 mmol) of pentacar-bonyliron, and is then irradiated. The photolysis is 20 carried out in a pyrex cell cooled by water circulation with a Philips HPK 125W lamp for 18 hours at a temperature in the region of 35°C. After evaporation of the solvent, the residue is purified by chromatography on a column of silica gel (Si02! 70-230 mesh; eluentj 25 CH2CI2), which yields a yellow oil (yield: 85%). Tri- carbonyl{l 4-n -[(IE, 3E)-l-alkoxycarbonyl^-methyl-S- oxo-l, 3-pentadienyl ] }irons are obtained in the same manner using the appropriate alkyl 2-(diethoxyphos-phoryl)acetate. PREPARATION 2: ETHYL (E)-4-DIETHOXYPHOSPHORYL- 3-METHYL-2-BUTENOATES STAGE A: ETHYL (E)-4-HYDROXY-3-METHYL-2-BUTENOATE 0.125 mmol of ethyl (E)-3-methyl-4-oxobu-35 tenoate, obtained in stage B, Preparation 1, dissolved in 100 ml of ethanol, is reduced with stirring and at a temperature below 20°C with an aqueous-alcoholic solution of 4.72 g (125 mmol) of sodium borohydride. Stirring is continued for 1 hour after the addition is - 11 - 22 933 i complete, the reaction medium is then filtered and the insoluble matter is washed with diethyl ether. After removal of the solvents, the residue is taken up with water and extracted with 3 x 100 ml of diethyl ether. 5 After drying and evaporation of the solvents, the oil is purified by passage through a column of silica gel (eluent: dichloromethane; SiC^: 70-230 mesh). Yield: 76% Oil STAGE B: ETHYL (E)-4-BROMO-3-METHYL-2-10 BUTENOATES Protected from moisture, with stirring and in the cold (0°-5°C), 10.5 g (39 mmol) of phosphorus tri-bromide are added dropwise (10 minutes) to an ethereal solution (75 ml) of 100 mmol of ethyl (E)-4-hydroxy-3-15 methyl-2-butenoate, obtained above. After a contact time of 1 h 30 min, the reaction medium is poured into water. The organic phase is separated and washed with saturated sodium hydrogen carbonate solution to neutrality. After drying and evaporation of the solvent, 20 the residue is chromatographed on a column of silica gel (eluent: dichloromethane; Sit^: 70-230 mesh). Yield: 91% Oil STAGE C: ETHYL (E)-4-DIETHOXYPHOSPHORYL-3-METHYL-2-BUTENOATE 25 50 mmol of ethyl (E)-4-bromo-3-methyl-2- butenoate are heated to 130-140°C with protection from moisture. After the heating has been stopped, 50 mmol of triethyl phosphite are added dropwise so as to maintain the temperature at about 130°C; the bromoethane 30 formed becomes removed as soon as it appears. When the addition is complete, the reaction is completed by heating for 30 minutes to 150-160°C. A distillation under vacuum enables the expected product to be collected. 35 Yield: 94% B.p.o: 150-152°C Alkyl (E)-4-diethoxyphosphoryl-3-methyl-2-bu-tenoates are obtained in an identical manner using in stage A the alkyl (E)-3-methyl-4-oxobutenoate corresponding to the expected product. - 12 - 22 9 3 3 1 EXAMPLE 1: ETHYL (E,E,E)-7-(6,8-DIMETHYL-2H-CHROMEN-3-YL)-3-METHYL-2,4,6-OCTATRIENOATE STAGE A: 6,8-DIMETHYL-3-ACETYL-2H-CHROMENE 5 1.50 g (10 mmol) of 2-hydroxy-3,5-dimethylbenz- aldehyde together with 0.66 g (10 mmol) of 3-oxo-l-butene are added at room temperature to a suspension of 0.14 g (1 mmol) of potassium carbonate in 50 ml of 2-butanone. The mixture is brought to reflux with stirr-10 ing and this temperature is maintained for 90 minutes. The reaction medium is evaporated on a water bath under vacuum, and the residue is taken up with 100 ml of water and extracted 3 times with 75 ml of diethyl ether. The organic phases are combined, washed to neu-15 trality with saturated aqueous sodium chloride solution, dried over sodium sulfate, filtered and evaporated on a water bath under vacuum, and chromatographed on a column of silica (70-230 mesh), eluting with dichloromethane. 20 Yield: 81%. STAGE B: 6,8-DIMETHYL-3-(1-HYDROXYETHYL)-2H-CHROMENE 2.02 g (10 mmol) of 6,8-dimethyl-3-acetyl-2H-chromene, obtained in the preceding stage, are dis-25 solved in 50 ml of ethanol. A solution of 0.38 g (10 mmol) of sodium borohydride in 5 ml of aqueous sodium hydroxide solution is added in a single portion with magnetic stirring. Stirring is maintained for 2 hours and the solvent mixture is then evaporated off. 30 The residue is taken up with 50 ml of water and extracted 3 times with 50 ml of diethyl ether. The ethereal solution is washed with saturated aqueous sodium chloride solution until the washing liquors are neutral, and the organic phase is dried and evaporated. 35 Yield: 86% STAGE C: [1-(6,8-DIMETHYL-2H-CHROMEN-3-YL)-ETHYL]TRIPHENYLPHOSPHONIUM BROMIDE 2.04 g (10 mmol) of 6,8-dimethyl-3-(1-hydroxy-ethyl)-2H-chromene, obtained in the preceding stage, - 13 - 22 9 3 3 1 and 3.43 g (10 iranol) of triphenylphosphonium bromide in 80 ml of methanol are placed under a nitrogen atmosphere at room temperature. The mixture is stirred for 96 hours. The residue obtained is removed on a column 5 of silica gel (70-230 mesh), using a methylene chloride/ethanol (95/5 v/v) mixture as elution solvent, and the product is crystallized in benzene. Yield: 68% Melting point: 164°C 10 STAGE D: ETHYL (E,E,E)-7-(6,8-DIMETHYL-2H- CHROMEN-3-YL)-3-METHYL-2,4,6-OCTATRIENOATE 70 ml of a solution of n-butyllithium in hexane (1.6 M) are added in the course of 10 minutes, with 15 stirring, at room temperature and under an inert atmos phere, to a suspension of 5.29 g (10 mmol) of [1—(6,8— dimethyl-2H-chromen-3-yl)ethyl]triphenylphosphonium bromide in 50 ml of tetrahydrofuran. Stirring is continued for 15 minutes and the mixture is cooled to a 20 temperature of between -15°C and -20°C. 3.06 g (10 mmol) of tricarbonyl{ 1—4-n-[ (IE, 3E)-l-ethoxycarbon-yl-2-methyl-5-oxo-l,3-pentadienyl]}iron, obtained in Preparation 1, are added in a single portion. The reaction medium is maintained at room temperature with 25 stirring for 18 hours. It is poured into 75 ml of water and extracted three times with 100 ml of diethyl ether. The organic phases are combined and washed to neutrality with saturated aqueous sodium chloride solution. The organic phase is dried over sodium sulfate 30 and filtered, and the diethyl ether is evaporated off. The product is purified by a chromatographic run on silica gel. The product obtained is then brought into contact with 11.15 g of trimethylamine N-oxide dihydrate 35 in 50 ml of methylene chloride. The mixture is heated under reflux for 5 hours. It is cooled, poured into 100 ml of water and extracted three times with 75 ml of dichloromethane. The organic phases are combined and 229331 - 14 - dried over sodium sulfate, and the solvent is evaporated off. The residue is purified by chromatography on silica gel (70-230 mesh), and the product obtained is recrystallized in diisopropyl ether. Yield: 20% Melting point: 122°C Elemental analysis: Calculated: C: 78.07 H: 7.74 Found: C: 77.41 H: 8.14 Spectral characteristics: Infrared Absorption at 1700, 1610, 1160 and 960 cm-1 Nuclear magnetic resonance (60 MHz) Solvent CDCI3 6= 1.30 ppm; triplet 3H CH3 ester J = 7Hz 5= 2.00 to 2.45 ppm; 4 singlets 4 x 3H 4CH3 (chromene + chain) 5= 4.25 ppm; quartet 2H CH2 ester J = 7Hz 6= 4.85 ppm; 2H CH2 chromene 6= 5.75 to 7.45 ppm; 7H; multiplet; aromatic and ethylenic. EXAMPLE 2: ETHYL 7-(2H-CHROMEN-3-YL)-3-METHYL- 2,4,6-OCTATRIENOATE STAGE A: 3-ACETYL-2H-CHROMENE 1.22 g (10 mmol) of salicylaldehyde together with 0.66 g (10 mmol) of 3-oxo-l-butene are added at room temperature to a suspension of 0.14 g (1 mmol) of potassium carbonate in 50 ml of 2-butanone. The mixture is brought to reflux with stirring and this temperature is maintained for 4 hours. The procedure is then as in Example 1, stage A. The final chromatography is replaced by a recrystallization in hexane. Yield: 57% Melting point: 58-60°C. STAGE B: 3-(1-HYDROXYETHYL)-2H-CHROMENE The procedure is as in Example 1, stage B, 6,8-dimethyl-3-acetyl-2H-chromene being replaced by 3-acetyl-2H-chromene, obtained in the preceding stage. Yield: 95% - 15 - 229331 STAGE C: [l-(2H-CHROMEN-3-YL)ETHYL]TRIPHENYLPHOSPHONIUM BROMIDE The procedure is as in Example 1, stage C, 6,8-dimethyl-3-(1-hydroxyethyl)-2H-chromene being replaced by 3-(1-hydroxyethyl)-2H-chromene. Yield: 98% STAGE D: ETHYL 7-(2H-CHROMEN-3-YL)-3-METHYL- 2,4,6-OCTATRIENOATE The procedure is as in Example 1, stage D. Crystallization solvent: dichloromethane Yield: 30% EXAMPLE 3: ETHYL (E,E,E)-7-(6-CHLORO-8-METHYL-2H-CHROMEN-3-YL)-3-METHYL-2,4,6-OCTATRIENOATE STAGE A: 6-CHLORO-8-METHYL-3-ACETYL-2H-CHROMENE Using the procedure described in Example 1, stage A, but with 2-hydroxy-3,5-dimethylbenz aldehyde replaced by 2-hydroxy-3-methyl-5-chlorobenzaldehyde, the expected product is obtained, and this is purified by an additional recrystallization in an isopropyl ether/petroleum ether mixture. Melting point: 78-79°C. STAGE B: ETHYL 3-(6-CHLORO-8-METHYL-2H-CHROMEN- 3-YL)-2-BUTENOATE A volume of dimethoxymethane sufficient to cover 0.108 g-at. of zinc is added into a round-bottomed flask equipped with a stirrer, a dropping funnel, a condenser and a thermometer. A few drops of pure ethyl bromoacetate are then added. The reaction is primed by gentle heating (with the flame of a lighter). The reaction is then maintained by the dropwise addition of 0.1 mmol of ethyl bromoacetate dissolved in anhydrous dimethoxymethane. The rate of addition is adjusted so that the temperature does not exceed 40°C. After the addition, the reaction medium is stirred for a further 30 minutes at room temperature, and 0.083 mmol of 3-acetylchromene, dissolved in anhydrous THF, is then added in a single portion. The reaction medium is stirred for a further 15 minutes and then - 16 - 22 9 3 3 hydrolyzed with saturated ammonium chloride solution. After 3 successive extractions with ethyl acetate, the organic extracts are dried over magnesium sulfate and the solvents removed. 50 mmol of the expected product 5 are dissolved in 50 ml of benzene and 1 ml of phosphorus oxychloride is added. The mixture is brought to reflux for 1 h 30 min. After being cooled, the reaction medium is poured into 100 ml of water. The benzene phase is decanted and the aqueous phase 10 extracted with 2 x 50 ml of diethyl ether. After the organic extracts have been washed with saturated sodium hydrogen carbonate solution to neutrality and dried and the solvents have been removed, the ester is purified on a column of silica gel (Si02: 70-230 mesh; eluent 15 CH2C12). Spectral characteristics: NMR 6: ppm (s: singlet; t: triplet; q: quartet; b.s.: broad signal) 1.30 (3H, t, J = 7Hz, OCH2CH3); 2.15 (3H, s, CH3 at 8-position); 2.45 (3H, b.s., CH3) 20 4.20 (2H, q, J = 7Hz, OCH2CH3); 4.95 (3H, s); 5.65 (1H, b.s., = I1 6.75, 6.90, 6.95 (3H, b.s., H4, H5 and H?) STAGE C: 3-(6-CHLORO-8-METHYL-2H-CHROMEN-3-YL)-25 2-BUTENOL Protected from moisture, with stirring and in the cold (-15, -10°C), a solution of 20 mmol of the ester obtained in stage B in 70 ml of diethyl ether (or tetrahydrofuran) is added dropwise to 20 mmol of 30 lithium aluminum hydride suspended in the same solvent. When the temperature has returned to room temperature, stirring is continued for 2 hours, and the excess hydride is then destroyed by the cautious addition of ethyl acetate and then water. The medium is then 35 acidified (pH 2-3). After drying and evaporation of the solvents, the crude product is directly subjected to oxidation. Yield: 95% - 17 - 22 9 3 3 1 Spectral characteristics: Infrared: 3400 \>OH 1610 vC = C 1235 vC = 0 5 STAGE D: (E)-3-(6-CHLORO-8-METHYL-2H-CHROMEN-3- YL) -2-BUTENAL A solution of 20 mmol of the chosen alcohol in 50 ml of dichloromethane is added slowly in the cold (-15, -10°C) and with stirring to 400 mmol of manganese 10 dioxide suspended in the minimum amount of dichloromethane. When the addition is complete, stirring is continued with cooling for 1 to 3 hours, and the medium is then filtered. After evaporation of the solvent, the product obtained is purified on a column of silica gel 15 (Si02J 70-230 mesh), eluting with dichloromethane. Yield: 20%. STAGE E: ETHYL (E,E,E)-7-(6-CHLORO-8-METHYL-2H- CHROMEN-3-YL)-2,4,6-OCATRIENOATE 3 ml of a hexane solution of n-butyllithium 20 (1.6 M) are added under a nitrogen atmosphere and at room temperature to 1.20 g (4.54 mmol) of ethyl (E)-4-diethoxyphosphoryl-3-methyl-2-butenoate, obtained in Preparation 2, dissolved in 10 ml of anhydrous THF. When the addition is complete, stirring is continued 25 for 15 minutes (reddish coloration of the reaction medium), and 0.91 g (4.54 mmol) of chromene aldehyde obtained in the preceding stage, dissolved in 5 ml of THF, is then added; stirring is maintained for 3 hours at room temperature. The reaction medium is then poured 30 into 100 ml of water and extracted with 3 x 25 ml of diethyl ether. After drying and evaporation of the solvents, the residue is chromatographed on a column of silica gel (2 chromatographic runs on Si02 70-230 mesh, eluent C^C^r and a third on Si02 230-400 mesh, eluent 35 CH2C12). 400 mg of a yellow solid are then obtained, and this is recrystallized in methylene chloride. Melting point: 107°C. - 18 - 22 9 3 3 1 EXAMPLE 4: ETHYL 7-(2H-CHR0MEN-3-YL)-3-METHYL-2,4,6-OCTATRIENOATE Using the procedure discovered in Example 3, but with 2-hydroxy-3-methyl-5-chlorobenzaldehyde 5 replaced by salicylaldehyde, the product of the title of Example 2 is obtained. In stage 3, the contact time will, however, be changed to 48 hours. Melting point: 107°C. 10 EXAMPLES 5 TO 9: By replacing in Example 1, stage A * 2-hydroxy-3,5-dimethylbenzaldehyde by: - 2-hydroxy-5-chloro-3-methylbenzaldehyde, ethyl (E,E,E)-7-(6-chloro-8-methyl-2H-chro- 15 men-3-yl)-3-methyl-2,4,6-ocatrienoate is obtained (EXAMPLE 5) - 2-hydroxy-5,6-dimethyl-4-methoxybenzaldehyde, ethyl (E,E,E)-7-(5,6-dimethyl-7-methoxy-2H-chromen-3-yl)-3-methyl-2,4,6-octatrienoate is 20 obtained (EXAMPLE 6) - 2-hydroxy-4-methoxybenzaldehyde, ethyl (E,E,E)-7-(7-methoxy-2H-chromen-3-y1)-3-methy1-2,4,6-octatrienoate is obtained (EXAMPLE 7) 25 - 2-hydroxy-5-methoxybenzaldehyde, ethyl (E,E,E)-7-(6-methoxy-2H-chromen-3-yl)-3-methyl-2,4,6-octatrienoate is obtained (EXAMPLE 8) - 2-hydroxy-3,5-dichlorobenzaldehyde, ethyl 30 (E,E,E)-7-(6,8-dichloro-2H-chromen-3-yl)-3- methyl-2,4,6-octatrienoate is obtained (EXAMPLE 9) The compounds of the Examples 5 to 9 may also be obtained using the procedure described for Example 35 3. PHARMACOLOGICAL STUDY: EXAMPLE 10: INHIBITION OF GROWTH OF L1210 LINE The growth of this mouse leukemic line is assessed by the capacity of the cells to incorporate - 19 - 22 9 3 3 1 tritiated thymidine. The degree of incorporation is measured 48 hours after the introduction of the test compounds into the culture medium. The compound No. 1 possesses, in this test, a 5 growth inhibition dose (ICgg) of 25m M, whereas that of etretinate is 80 uM. EXAMPLE 11: STUDY OF THE TOXICITY ON THE HUMERI OF RAT FETUSES OF GESTATIONAL AGE 21 DAYS 10 The toxicity of retinoids can be evaluated on humeri of rat fetuses explanted in vitro as described by KISTLER (1985). Retinoid activity results in a release of proteoglycans from the bone matrix. This release is 15 assessed after 7 days of culture in vitro by assaying the concentration of proteoglycans in the medium, by the method of WITHEMAN (1973). The compounds of the present invention prove completely incapable of stimulating the release of 20 proteoglycans from the bone matrix of the humeri of rat fetuses at a concentration of 100 u M, whereas etretinate permits a significant release of these proteoglycans at a dose of 40 u M. EXAMPLE 12: DETECTION OF AN EXCESS-VITAMIN A 25 TYPE TOXICITY IN VIVO The side effects limiting the use of retinoid agents in human clinical medicine are reflected in the appearance of a hypervitaminosis A syndrome; this can be reproduced experimentally in animals. 30 The selected treatment protocol is a daily injection for five days, repeated a second time after an interval of two days. It is identical to that used by BOLLAG et al (1981). Whereas retinoic acid and etretinate cause a 35 rapid weight loss, the appearance of alopecia and bone weakening, manifestations of their toxicity, compounds of the present invention proved devoid of excess-vitamin A type toxicity: no statistically significant sign in respect of weight, no fracture detectable by </div>

Claims

<div class="application article clearfix printTableText" id="claims"> 22 9 3 3 1 - 20 - radiography and no onset of alopecia were reported following treatment with the compounds of the invention. 22 9 3 3 1 21 MtAT./tfWE CLAIfwl tt» 1. The compounds of general formula: R R 2 3 (I) R '4 in which: R^, R2, R3 and R4 , which may be identical or different, denote a hydrogen atom, a halogen atom or a lower alkyl, lower alkenyl, lower alkyloxy or lower alkenyloxy group, optionally substituted with one or more halogen atoms, R5 denotes a carboxyl, (lower alkyloxy)carbonyl, (lower alkenyloxy)carbonyl or (lower alkynyloxy)-carbonyl, their isomers, stereoisomers and diastereoisomers, and also their addition salts with a pharmaceutically acceptable base when Rg denotes a carboxyl group.
2. A compound as claimed in claim 1, in which the configuration is (E,E,E).
3. A compound as claimed in one of claims 1 and 2, which is ethyl (E,E,E)-7-(6,8-dimethyl-2H-chromen-3-yl)-3-methyl-2, 4,6-octatrienoate.
4. A compound as claimed in one of claims 1 and 2, which is ethyl (E,E,E)-7-(2H-chromen-3-yl)-3-methyl-2,4,6-octatrienoate.
5. A compound as claimed in one of claims 1 and 2, which is ethyl (E,E,E)-7-(6-chloro-8-methyl-2H-chromen-3-yl)-3-methyl-2,4,6-octatrienoate.
6. A compound as claimed in one of claims 1 and 2, which is ethyl 7-(2H-chromen-3-yl)-3-methyl-2,4,6-octa- £. i— , ^ i - 22 - trienoate.
7. A process for preparing compounds of formula (I) as claimed in claim 1, wherein a derivative of formula (II): (II) in which R1, R2/ R3 and R4 have the same meaning as in the formula (I), is condensed by heating under reflux of the solvent with 3-oxo-l-butene of formula (III); (III) in the presence of an alkaline agent in a polar solvent to lead, after cooling and, where appropriate, evaporation of the reaction medium, taking up with water to which an alkaline agent has been added —, where appropriate, extraction with a suitable organic solvent washing of the organic phase, evaporation of the solvent and purification of the residue by crystallization or distillation or chromatography on a 22? - 23 - silica or alumina column, to a derivative of formula (IV): (IV) in which Rlf R2, R3 and R4 have the same meaning as in formula (I), which derivative is subjected to catalytic hydrogenation, after dissolution in a suitable organic solvent, "the hydrogenation— — : 'being carried out with an alkali metal mixed : to lead, hydride : after extraction with a suitable organic solvent preceded where appropriate by evaporation of the reaction medium, washing, then evaporation of the organic phase and purification, to a derivative of formula (V): in which Rj_, R2, R3 and R4 have above, which derivative is subjected to the action of a triphenylphosphonium salt, at room temperature and with stirring, to lead, after evaporation of the solvent and purification by chromatography on silica gel or by crystallization in a solvent or a mixture of - 24 - o 9 ° 7 ^ 1 L- L, * 4 suitable solvents, to a derivative of formula (VI): R CH3 P®—(C,H,)3 (VI) R A © R4 in which A" denotes the anion of a hydracid and R^, R2/ R3 and R4 have the same meaning as above, which is subjected, in a suitable solvent,a under an inert atmosphere and in the presence of n-butyllithium in hexane, to the action of a stereochemical^ blocked dienic aldehyde in the form of a tricar-bonyliron complex of formula fVII}: in which Rg has the same meaning as in the formula (I), at a temperature of between -5°C and -30°C, to.lead, after extraction with a suitable solvent, washing and complex which, after purification by chromatography, is subjected, under reflux of the organic solvent chosen for this stage, to the action of trimethylamines with N-oxide, to lead, after cooling, extraction with a suitable organic solvent, washing and evaporation of the organic phase and column chromatography, to a compound of formula (I), which can, if so desired, evaporation of the organic phase, to an intermediate 229331 - 25 - - either, when R5 denotes a carboxyl group, be salified with a pharmaceutically acceptable base, - or be separated into its stereoisomers by a technique of crystallization or chromatography and then, if so desired, when Rg denotes a carboxyl group, be salified with a pharmaceutically acceptcible base.
8. The preparation process as claimed in claim 7, wherein a derivative of formula (II): (II) in which Rlf R2, R3 and R4 have the same meaning as in the formula (I), is condensed by heating under reflux of the solvent with 3-oxo-l-butene of formula (III): (HI) in the presence of an alkaline agent in a polar solvent, to lead, after cooling and, where appropriate, evaporation of the reaction medium, taking up with water to which an alkaline agent has been added where appropriate, extraction with a suitable organic 229331 - 26 - solvent washing of the organic phase, evaporation of the solvent and purification of the residue by crystallization or distillation or chromatography on a silica or alumina column, to a derivative of formula (IV): R. CH, (IV) R4 in which R^, R2, R3 and R4 have the same meaning as in formula (I), which derivative is subjected to the action of ethyl bromoacetate in the presence of zinc, followed by a hydrolysis of the reaction medium at the required moment and extraction with a suitable organic solvent , and then to the —— action of phosphorus oxychloride in a suitable organic solvent, evaporation of the reaction medium and a further extraction with a solvent : to lead, where appropriate after chromatography on silica gel, to a derivative of formula (VIII): (VIII) R4 in which R^, R2, r3, and R4 have the same meaning as in the formula (I), which is subjected at a low temperature between -50 and 0°C, in a solvent- 27 to the action of —— lithium aluminum hydride, to lead to a derivative of formula (IX)s in which , R2, R3 and R4 have the same meaning as in -the formula (I), which is subjected to oxidation with manganese dioxide to lead, after chromatography on silica gel and crystallization where appropriate, to a derivative of formula (X)s in which Rj, R2, R3 and R4 have the same meaning as in the formula (I), which is subjected to the action of a derivative of formula (XI): (IX) (X) 22 > O -7 7 1 l - 28 - in which Rg has the same meaning as in the formula (I) and Z is a lower alkyl group, at room temperature, to lead, after extraction with a suitable organic solvent, evaporation and purification by chromatography on a silica column, to a compound of formula (I), which can, if so desired, - either, when Rg denotes a carboxyl group, be salified with a pharmaceutically acceptable base, - or be separated into its stereoisomers by a technique of crystallization or chromatography and then, if so desired, when R«j denotes a carboxyl group, be salified with a pharmaceutically acceptable base.
9. A pharmaceutical composition containing, at least one compound as claimed in one of claims 1 to 6, in combination with one or more pharmaceutically acceptable, non-toxic, inert vehicles or excipients.
10. The pharmaceutical composition as claimed in claim 9, containing at least one compound as claimed in one of claims 1 to 6, usable in the treatment of tumors and neoplasms as well as in various skin disorders.
11. The compound of general formula I as claimed in claim 1 \ substantially as herein described with reference to the examples.
12. A process for preparing compounds of formula I as claimed in claim 1 substantially as herein described with reference to the examples. <£-r Sy >fff/th@fr authorised Agents A. J. PARK & SON. </div>