NZ205291A - (1-oxo-2-pyridyl)-disulphide derivatives and animal feed compositions containing them - Google Patents

Abstract

Selected (1-oxo-2-pyridyl) disulfides are used as active ingredients in animal feed compositions and in methods for increasing the growth and feed efficiency of monogastric animals. A useful new compound of this invention is 2-carboxyphenyl (1-oxo-2-pyridyl) disulfide.

Description

New Zealand Paient Spedficaiion for Paient Number £05291 205291 Priority Date(s): . R.~.

Complete Specification Filed: Class: .. ft??.*J jib.

Publication Date: ... • • P.O. Journal, No: .

ZEALAND PATENTS ACT, 1953 No: Date: COMPLETE SPECIFICATION FEED COMPOSITIONS CONTAINING A (1-0X0-2-PYRIDYL) DISULFIDE we, SMITHLKINE BECKMAN CORPORATION, a corporation organized under the laws of the Commonwealth of Pennsylvania, one of the United States of America, of One Franklin Plaza, Philadelphia, Pennsylvania 19103, United States of America hereby declare the invention for which we pray that a patent may be granted to us, and the method by which it is to be performed, to be particularly described in and by the following statement: (followed by page -la-) 1 2052 9 1 - lo- FEED COMPOSITIONS CONTAINING A (1-QXO-2-PYRIDYL) DISULFIDE This invention comprises new animal feed compositions and methods using a (l-oxo-2-pyridyl) disulfide as an active ingredient for altering the metabolic pattern in the digestive tract of monogastric animals, thereby, improving growth and feed efficiency of the animals.

Pyridyl disulfides have been described to be good topical antibacterial or antifungal agents for use in shampoos, wound dressings or soaps. Unsymmetrical disulfides for this use are disclosed in M. L. Douglas, U.S. Patent No. 4,049,665. Column 7, line 66 of the Douglas patent describes the concentration of disulfide in such topical compositions to be from 0.1-99%.

The animal feed compositions of this invention, which are supplemented by a (1-oxo-pyridyl) disulfide, are fed to monogastric, growing or fattening, meat-producing animals, especially swine and poultry.

For example, it is known to the art that, during assimilation of food, the production of volatile fatty acids and lactic acid in swine should be relatively low in the upper part of the digestive tract. On the other hand, glucose levels should be higher in the upper tract.

Lysine is an essential amino acid which is necessary for growth. Therefore, high levels of lysine are also 20529 1 desirable. Often, corn diets, which are naturally low in lysine, are supplemented with lysine.

The object of this invention is, therefore, to induce relatively low volatile fatty acid production in 5 the upper digestive tract together with a high glucose level, high lysine level and low lactic acid level by administering orally a nontoxic but effective quantity of a selected disulfide to the subject animals. This combination of factors is desired to insure that a larger 10 than normal quantity of energy and amino acid is available to the animals for growth from each unit of food in their diet.

The active ingredients, which are useful in the feed compositions and methods of this invention, are 15 illustrated by the structural formula: in which: R and R^~ are, each, hydrogen, C^_g-alkyl, C^_g-alkoxy, C^_6~alkylthio, nitro, hydroxy, halo such as chloro or bromo, carboxy, phenyl, benzyl or benzylthio; and 2 R is phenyl, benzyl, pyridyl or 1-oxopyridyl, each of which has one or two substituents as defined for R and R^" above, C-^_^2~alkyl/ furyl, thienyl, glucosyl, (^..-^"alkeny! or carboxy-C^_g-alkyl.

A subgeneric group of preferred compounds to be used as active ingredients in this invention are those of 2 formula I in which R is not a pyridyl or 1-oxopyridyl. These may be described as unsymmetrical (l-oxo-2-pyridyl) 35 disulfides. 2052 Another aspect of this invention is the group of new chemical compounds which are represented by the following structural formula: R1 ^ S-S-P-CO-H II N.^ 2 + 0 R and R1 are, each, hydrogen, C-^_g-alkyl, C^_g-alkoxy, C^_g-alkylthio, nitro, hydroxy, halo such as chloro or bromo, carboxy, phenyl, benzyl or benzylthio; and P is alkylene of from 1-6 carbons or phenylene. The active ingredients of formulas I and II are prepared conveniently by the following reaction: ^XRl 2 + X-S-R + I or II R .SH + 0 12 in which R, R or R are as defined above; and X is chloro or bromo. 2 The sulfenyl halide, X-S-R , is conveniently prepared and reacted, in situ, with the mercaptan starting material in an inert organic solvent at a temperature selected from the range of from 0° to the boiling point of the reaction mixture until completion. Base is added to the reaction material to neutralize the reaction product in order to isolate the free base form of the disulfide product. The base or the acid addition salt form of the disulfide, the latter preferably generated from the 20529 sulfenyl halide condensation which is outlined above, may be obtained by conventional isolation methods.

Alternatively, certain disulfide active ingredients used in this invention can be prepared by a sulfur/sulfur interchange reaction as described in the examples hereafter.

Also, included in this invention as active ingredients are the nontoxic, stable salts of the compounds of formulas I and II with acids known to the art to be useful for such purposes, for example, the hydrochloride, sulfate, sulfamate, phosphate, nitrate or acetate salts. The salt form, as noted above, may either be formed during the chemical reaction used in the preparation of the chemical ingredients or as a separate step, such as by reacting a compound of formula I with an excess of acid in an organic solvent. Also, if a carboxy group is present in the disulfide as in formula II compounds, a nontoxic alkali metal salt may be formed such as by shaking the disulfide with dilute potassium hydroxide, filtering and lyophilizing the filtrate. One skilled in the art will, therefore, recognize that certain compounds which are the active ingredients of this invention will have the capability to form salts and how such salts are prepared and isolated.

In addition to the salt forms of the compounds of this invention, other latentiated or prodrug forms of the active ingredients of formulas I and II may be used such as C^_g-alkyl or phenyl esters of the acid derivatives which are convertible within the animal's body to the active free acid parent. A specific example of such a latentiated derivative, which has been found to be very active as an active ingredient of this invention, is the 4-nitrophenyl ester of 2-carboxyethyl (l-oxo-2-pyridyl) disulfide.

The feed compositions of this invention comprise the normal feed rations of the meat producing animals 205291 supplemented by a quantity of an active ingredient of formula I, which is effective for improving the growth rate and feed efficiency of the animals but which is not toxic or noxious to a degree that the animals will reduce ingestion of the ration. The quantity of the active ingredient will vary, as is known to the art, with factors such as the cost of the ingredient, the species and the size of animal, the relative activity of the compound of formula I or the type of feed ration used as the basal feed.

Representative feed rations for swine and poultry are in the examples presented hereafter. Swine feed from weanling to fattening or finishing rations may be supplemented. Swine eat from about 2 lb. per day (for a 25 lb. pig) to 9 lb. per day (for a 150 lb. pig). Most rations are comprised of a corn base supplemented with legume silage, wheat bran, oats, barley, molasses or a protein supplement.

Poultry feeds comprise starter rations, broiler rations and laying rations. The rations are usually based on ground corn, corn meal or soybean meal. The broiler rations, often, contain high energy supplements such as added fats, proteins and vitamins. Turkey rations are similar, but comprise only a starting ration and a growing ration. Chickens or pheasants eat from .03-0.3 lbs. of feed per day, turkeys twice that much. Estimated intake of feed is dependent on the weight and age of the meat producing animal.

The active ingredients of formula I are mixed uniformly with such feed rations to give supplemented rations which are, then, fed as to custom which, is, most often, ad libitum. Conveniently, to do this, a premix of the supplemental growth promotant of this invention, optionally combined with or without other supplements known to this art such as an anthelmintic, a nitrogen source or an antibiotic such as virginiamycin or 2052 oxytetracycline, is prepared by the manufacturer for sale to the formulators or feed lot operators. The concentration of disulfide in the premix is usually from 5-75% by weight or a concentration 100-2000 times greater than that in the complete feed ration. The premix form may be liquid or solid. Premix vehicles are corn oil, cottonseed oil, molasses or distillers solubles to form a liquid premix preparation. Sucrose, lactose, corn meal, ground corn, flour, calcium carbonate or soybean meal are often used as bases for solid premix preparations. The premix composition is, then, mixed uniformly with whole ration which is commonly fed to the target animal. Such premix compositions are included in the term "feed compositions" as used herein.

The concentration of the disulfide of formula I in the complete ration is a nontoxic but active quantity chosen, for example, from a range of about 1-100 parts of active ingredient by weight per million parts of whole feed (ppm) or about 2-115 grams per ton. Advantageously, a quantity is chosen from the range of 5-50 ppm of a disulfide of formula I.

The percentage ratio by weight of active ingredient to feed suggested for this invention is selected from the range of about 0.0001-0.01%.

The method of using compositions of this invention comprises feeding them to growing, monogastric, meat-producing animals, especially swine and poultry, in an effective growth promoting but nontoxic quantity of such a compound having formula I. Other monogastric animals whose digestive tract features fermentation in a cecum or cecum-like chamber are rabbits and horses.

The supplemented feed rations described above are presented to the animal by methods known to the art. Ad libitum feeding in the pasture, pen or growing shed is most convenient to increase the growth rate of the animal and to increase the feed efficiency of the growing opera- 2052 9 tion. Alternatively, the same supplemented feeds may be given to ruminant animals, particularly when the disulfide of formula I is coated to bypass the upper stomach or rumen. Data presented in the in vitro working examples demonstrate that a selective effect as described above was not present in the rumen.

For example, a species active ingredient of this invention, 2-carboxyphenyl (l-oxo-2-pyridyl) disulfide, facilitated growth in chickens and pigs by as much as 6% with a 5-10% improvement in feed efficiency when used as described herein. A concentration of this species in whole feed ration which has proved very effective is about 15-25 ppm. 2-Carboxyethyl (l-oxo-2-pyridyl) disulfide gave similar results.

The following working examples are intended to illustrate this invention. All percentages are by weight. All temperatures are Centigrade.

EXAMPLE 1 A swine ration for growing hogs of 40-100 pounds body weight is prepared using the following formula: Corn, ground 78.15% Soybean oil meal, 44% 17.0% Meat scraps, 50% 3.0% Oyster shell flavor 0.4% Bone meal 0.5% Zinc oxide 0.01% Vitamin A, B, B^2 & D optional supplement The ration is supplemented to 100% with 20 ppm of 2-carboxyphenyl (l-oxo-2-pyridyl) disulfide distributed through a premix carrier. The ration is fed, ad libitum, to the penned growing or fattening swine. 205291 EXAMPLE 2 A chicken ration for broilers is prepared using the following formula: Yellow corn meal 67.

% Soybean oil meal 24. 00% Menhaden fish meal 6. 00% Steamed bone meal 1. 00% Ground limestone 1. 00% Iodized salt 0. 34% % choline chloride 0. 13% Vitamin 0.

% Manganese sulfate 0. 02% Vitamin mix 0. 06% The ration is supplemented with 20 ppm of 2-carboxyphenyl (1-oxo-N-pyridyl) disulfide and fed ad libitum to the chickens.

EXAMPLE 3 In Vitro Swine Procedure A. Methodology: A Yorksire barrow is surgically prepared either with an ileal cannula, which is placed 15 cm. from the ileo-ceco-colic junction, or a cecal cannula, which is placed midway between the apex and origin of the cecum. The animal is fed 4 times daily to restrict intake to 4.5% of body weight in a 30 kg animal or 2.5% of body weight in a 100 kg animal. The swine grower ration is: % w/w lbs/ton Medium ground shelled corn 70.60 1412 Soybean meal, 44% 22.00 440 Dehydrated alfalfa meal, 17% 4.50 90 Calcium propionate 0.15 3 Vitamin/mineral premix 2.75 55 2052 Sampling of the material, via the cannula, begins 150-180 minutes following the first morning feeding and continues any time from 30-120 minutes thereafter, depending on the quantity of material needed. The sample is maintained in crushed ice, no cooler than 5°, and is gassed continuously with carbon dioxide. The collected material is filtered. The filtrate is the inoculum used for incubations of the test and control samples. The gassed inoculum, 2.25 ml, is placed in each of 10 gassed test tubes, each containing 0.75 ml of a nutrient solution and 0.5 mg of each test compound. Four blank control tubes, along with the test compound tubes, are incubated 5 hours at 37° with agitation. Four more killed tubes are included which are not incubated.

The tubes are each treated with 0.60 ml of a 25% solution of metaphosphoric acid, then, stored at -4° until analysis. Samples are thawed and centrifuged for 25 minutes at 20,000 r.p.m. The supernatent liquid is decanted, sampled for gas chromatography and automatic analyzing. The results are fed into a computer for finishing to give figures in which the blank control value is 100%. Virginiamycin is used as a positive control.

B. Results: Compound VFA LYS GLU LAC* 1. 2-Carboxyphenyl (l-oxo-2-pyridyl) disulfide % of control values Ileal a) 166.7 ppm 49 107 999 129 b) 166.7 ppm 57 160 235 79 c) 166.7 ppm 36 176 190 100 d) 166.7 ppm 385 152 * VFA refers to the total of volatile fatty acids, namely acetate, propionate, isobutyrate, butyrate, isovalerate and valerate. LYS is lysine, GLU is glucose and LAC is L-lactic acid. 20529 1 Compound VFA LYS GLU LAC % of control values Representative Virginiamycin control runs Ileal a) 166.7 ppm 39 289 153 23 16.7 ppm 85 212 153 21 1.67 ppm 147 134 131 39 4-Fluorophenyl (l-oxo-2-pyridyl) disulfide hydrochloride Ileal a) 166.7 ppm 19 246 181 106 b) 166.7 ppm 57 200 370 56 c) 166.7 ppm 56 104 925 142 (2,3,4,6- Tetra-O-acetyl- -D-glucosyl) ( 1-OXO-2-; pyr idy: disulfide hydrobromide Ileal a) 166.7 ppm 64 174 224 78 b) 166.7 ppm 147 144 105 c) 166.7 ppm 29 123 139 104 Rumen (Fistulated cattle) d) 50.0 ppm 70 83 0 364 .0 ppm 104 96 28 249 0.5 ppm 108 128 0 107 S-D-Glucosyl (l-oxo-2-pyridyl) disulfide dihydrate Ileal a) 166.7 ppm 23 321 152 52 16.7 ppm 42 294 152 47 1.67 ppm 106 114 119 78 b) 166.7 ppm 29 107 117 102 Dodecyl (l-oxo-2-pyridyl) disulfide Ileal a) 166.7 ppm 39 148 159 105 16.7 ppm 50 127 138 107 1.67 ppm 68 134 118 104 b) 166.7 ppm 26 126 0 141 205291 1 Compound VFA LYS GLU LAC 6. (5-Bromo-l-oxo-2-pyridyl) disulfide % of control values Ileal a) 166.7 ppm 69 81 353 150 16.7 ppm 80 90 250 110 1.67 ppm 82 92 155 101 b) 166.7 ppm 44 122 156 135 c) 166.7 ppm 49 119 130 125 d) 166.7 ppm 103 94 134 94 7. (l-Oxo-2-pyridyl) disulfide Ileal a) 166.7 ppm 212 203 70 b) 166.7 ppm 17 206 180 16.7 ppm 19 185 180 1.67 ppm 93 121 113 c) 166.7 ppm 4 143 133 d) 166.7 ppm 41 145 625 118 16. 7 ppm 39 134 649 118 1. 67 ppm 84 110 415 110 Rumen e) 50.0 ppm 16 197 999 518 f) 50.0 ppm 65 333 17 8. 2-Mercapto-l-oxopyridine Ileal a) 333. 3 ppm 129 100 101 b) 166.7 ppm 19 175 505 94 c) 166.7 ppm 31 100 138 101 C. Conclusions: These representative results demonstrate the pro jected spectrum of biological activity of the disulfides, especially compared with that of the parent 1-oxopyridine mercaptan (8). Inhibition of volatile fatty acid formation is particularly to be noted. Similar studies in 35 the rumen of fistulated cattle, certain of which are noted above, demonstrate that the selective effect is limited to monogastric animals. 205291 EXAMPLE 4 Chick Growth Study A. Methodology: 512 one day old broiler chicks, selected for weight, health and sex, are housed in an environmentally controlled room with temperature at 80°F and humidity at 40%. Chicks are fed ad libitum. Water is offered ad libitum. A rye or corn basal ration is fed during the acclimation period (days 1 and 2), then, mixed with the compound under test or control conditions on days 3-17. Either 8 or 16 chicks are used for each test or control group.

Basal Rye Diet Diet Ingredients (% w /w) (lbs/ton) Ground Rye (fine grind) 54. 6 1092 Soybean Meal (49% protein) 27 540 Meat & Bone meal (50% protein) 200 Dehydrated Alfalfa meal 1.

Fat, animal 4 80 Dried Whey (or lactose) 1 Ground Limestone 0. 67 13.4 Dicalcium Phosphate 0. 50 Iodized salt 0. 23 4.6 Vitamin premix 0. 175 * Trace mineral premix 0.

DL methionine (98%) 0.

Choline Chloride (50% aqueous sol.) 0.150** 3 * Vitamin premix will be mixed into diets when test chemicals are added. 87.5 g vitamin premix/49,912.5 g of basal rye diet.

** Since choline is added as a 50% aqueous solution, percentage in diet is doubled. 20529 1 B. Results: % of Control Chemical Weight (17 day) Feed/Gain (3-17 day) 1. Virginiamycin a. 10 ppm (rye) b. 50 ppm (rye) c. 10 ppm (rye) 107.1 127.8 97.7 2. l-Oxo-2-pyridyl disulfide 2.5 ppm 1 ppm 10 ppm 1 ppm 10 ppm 20 ppm 1 ppm 10 ppm f. 2.5 ppm g. 2.5 ppm a. b. d. e. (corn) (rye) (rye) (rye) (rye) (rye) (rye) (corn) (corn) 102. 2 100. 2 94.7 97. 96, 96, 100, 94 102, 6 2 2 7 2 96.5 88.5 100. 4 111. 4 98. 2 101.9 95.4 103. 96, 98, 101, 114, 1 6 2 9 4 103.0 99.8 (31 days) 3. 4-Fluorophenyl (1-oxo-2-pyridyl) disulfide hydrochloride a. 5 ppm (rye) b. 10 ppm (rye) c. 5 ppm (rye) 99.4 98.0 99.4 87.5 97.8 87. 5 a. b. 2-Carboxyphenyl (l-oxo-2-pyridyl) disulfide ppm (rye) 2 ppm (rye) ppm 10 ppm 50 ppm c. 5 ppm (rye) ppm 50 ppm d. 10 ppm (rye) 50 ppm 100 ppm e. 10 ppm (rye) f. 6 ppm (rye) 2,3,4,6-Tetra-0-acetyl-B-D-glucosyl (l-oxo-2-pyridyl) disulfide hydrobromide 103.6 100.1 100.7 102. 2 101.6 100. 6 102.8 101.3 98.6 102.6 97. 2 100.0 99.8 96.6 86.9 107.5 104.2 94. 2 105.3 88.8 54.1 179.1 99.5 98.8 106.0 94.7 a. 10 ppm (rye) 95. 4 101.6 205291 1 Conclusions: The tests in chickens confirm the enhancement of either growth rate or increase in feed efficiency of the poultry feed compositions. The 17 day growth rate should 5 be positive and the 3-17 day feed efficiency should be negative. Certain test results are negative due to biological variability in our early or initial screens.

EXAMPLE 5 A. Methodology: A standard corn based, pre-starter feed composition for feeding 2 week old post weanling pigs was supplemented with 20 ppm of the bis(oxo-2-pyridyl) disulfide. The animal group comprised 15 replications, each 15 containing 3 treatments, which were untreated corn ration as negative control, virginiamycin at 20 ppm as positive control and the stated test ration. Each replication contained 8 treatment groups, in turn, comprising 2 pigs per pen.

The following data summarizes the results: B. Results: Control Virginiamycin (l-oxo-2-pyridyl) disulfide Dose (ppm) - 20 10 Starting wt (Kg) 22.3 23.4 22.2 Average daily gain .720 + .066 .730 + .066 (0-21 day) Average daily gain .725 .746 .716 Feed/gain 2.95 2.80 2.84 (0-41 day) .verage dai Feed/gain (0-63 day) Average daily gain .749 .747 .723 Feed/gain 3.36 3.32 3.26 2052 91 EXAMPLE 6 A. Methodology: Replications of swine each containing 3 treatments test compositions containing 20 ppm of 2-carboxyphenyl (l-oxo-2-pyridyl) disulfide (CPD).

B. Results: Control Virginiamycin CPD Dose - 10 ppm 20 ppm Starting wt 23.7 22.51 23.17 Average daily gain .704 .697 .690 Feed/gain 2.70 2.61 2.65 (0-21 day) Average daily gain .691 .719 .720 Feed/gain 2.85 2.82 2.83 (0-35 day) C. Conclusions: 2-Carboxyphenyl (l-oxo-2-pyridyl) disulfide (CPD) demonstrated increased growth and feed efficiency roughly 20 equivalent with that of virginiamycin.

EXAMPLE 7 A. Methodology: Ten Yorkshire barrows were fed a standard ration (5 pigs) or the same ration supplemented with 100 ppm of 2-25 carboxyethyl (l-oxo-2-pyridyl) disulfide (5 pigs) for 21 days. Pigs were paired into 5 replicates based on starting weight and rate of gain from delivery to the feeding site until the study began.

B. Results: Ave. Ave. Ave.

Starting Final Daily n wt. (kg) wt. (kg) Gain (kg) Feed/Gain Control 5 28. 2 44. 05 0. 755 2.851 27.8 44.0 0. 771 (102. 2%) 2.701 (94.8%) 205291 C. Comments: No outward toxic effects were observed.

EXAMPLE 8 ^ Chemical Preparations A. A round bottom flask equipped with mechanical stirrer was charged with 3.08 g (0.020 mol) of 2-mercapto-benzoic acid and 100 ml of anhydrous ether. The mixture was stirred until the thiosalicylate dissolved. To this ^ solution was added 4.45 g (0.025 mol) of N-bromosuccini- mide. After 60 minutes, the orange solution which contains 2-carboxybenzenesulfenyl bromide was decanted from the succinimide precipitate. To the orange etherate solution was added 2.5 g (0.020 mol) of 2-mercaptopyridine-N-oxide.

After 90 minutes of vigorous agitation at 25°, a 85% yield of 2-carboxyphenyl (l-oxo-2-pyridyl) disulfide was collected by filtration. The disulfide had a melting point of 145-147°. Compound is further purified by recrystallization from 8:1:1:1 (8 parts methanol to 1 part methylene chloride, ether and acetic acid respectively) solution. The purified compound had a melting point of 145-146°.

Anal. Calcd. for C12HgN03S2: C, 51.60; H, 3.25? N, 5.01; S, 22.95. Found: C, 51.47; H, 3.28; N, 5.12; S, 22.82.

The above procedure is repeated using 3-mercapto-benzoic acid and 4-mercaptobenzoic acid to give, respectively, 3-carboxyphenyl (l-oxo-2-pyridyl) disulfide and 4-carboxyphenyl (l-oxo-2-pyridyl) disulfide.

^ B. In a round bottom flask was placed 500 mg (0.0027 mols) of N-bromosuccinimide, 1 g (0.0028 mol) of g-thioglucose tetraacetate and 50 ml of toluene. The mixture was mechanically stirred for 20 minutes while the temperature was lowered to 0°. To this cooled solution is "?5 added, from a dropping funnel, 340 mg (0.0027 mol) of 2- 205291 mercaptopyridine-N-oxide in 50 ml of toluene. After 20 minutes at 5° or below, the temperature was raised to 60° and stirring was continued for an additional 20 minutes. The reaction mixture was cooled to room temperature and stirred overnight. A white precipitate was collected and thin layer chromatography showed one spot (using a silica gel plate with eluting solvent of 8:1:1:1 methylene chloride:methanol; ethyl ether; acetic acid). Crude metling point was 160-163°. The product was further purified by recrystaliization from methanol and anhydrous ether (1:2 ratio). The final product was vacuum oven dried for 24 hours to give preparation of (2,3,4,6-tetra-O-acetyl-g-D-glucosyl) (l-oxo-2-pyridyl) disulfide hydrobromide, m.p. 154-155°.

Anal. Calcd. for C-^gH2-jNOj_gS2*HBr: C, 40.00; H, 4.24; N, 2.45. Found: C, 39.70; 40.12; H, 4.17, 4.17; N, 2.26, 2.39.

C. (2,3,4,6-Tetra-O-acetyl-6-D-glucosyl) (1-oxo-2-pyridyl) disulfide (250 mg) was deacetylated by dissolving the compound in methanol and bubbling in hydrogen chloride gas. After 10 minutes, the gas was removed and mixture stirred overnight. Methanol was removed by rotary evaporation and clear solid is dried under nitrogen vacuum. The compound has a melting point of 87°; ( $-D-glucosyl) (1-oxo-pyridyl) disulfide dihydrate.

Anal. Calcd. for C,, H, .-NO.-S,, • 2. 5 H„0: 11 15 6 2 2 C, 36.06; H, 4.95; N, 3.82. Found: C, 36.11; H, 4.65; N, 3.78.

D. N-chlorosuccinimide (4.39 g) was added in several portions to a cold (0°) solution of 4.26 g (0.032 mol) of 97% £—fluorothiophenol and 100 ml of toluene.

After 40 minutes stirring at room temperature, the orange solution was collected by filtration, cooled to 0° while a solution of 2.11 g of mercaptopyridine-N-oxide in 100 ml of ether was added. After stirring at room temperature

Claims (1)

10 20 0 5291 - 18 - for 20 minutes, the product was collected, washed and purified over a silica gel column to give 5.27 g of 4-fluorophenyl (l-oxo-2-pyridyl) disulfide, m.p. 94-96°. Anal. Calcd. for C^HgNOSF • HC1: C, 52.16; H, 3.18; N, 5.53. Found: C, 52.07; H, 3.27; 5.63. E. Using the same procedure as above, dodecyl (1-oxo-2-pyridyl) disulfide hydrobromide was obtained; m.p. 103-105°. Anal. Calcd. for C^H2gNOS2 * HBr: C, 42.97;;H, 7.40; N, 3.43. Found: C, 50.40, 50.28; H, 7.07, 7.30; N, 3.40, 3.38.;F. A mixture of 8.6 g (0.034 mol of bis(l-oxo-2-pyridyl)-disulfide, 6.9 (0.056 mol) of 3-mercaptopropionate and 400 ml of chloroform was heated at reflux overnight.;^ Cooling separated a product contaminated with disulfide starting material. The mixture was purified by recrystaliization from hot methanol to give 2-carboxyethyl (l-oxo-2-pyridyl) disulfide, m.p. 162-163°.;25;Anal. Calcd. for CgHgN02S2: C, 41.56; N, 3.89; N, 6.06; S, 27.70. Found: C, 51.54; H, 3.94; N, 6.05; S, 27.85.;The 4-nitrophenyl ester derivative melts at 109-;111°.;Anal. Calcd. for (-^^H^2N2<^5S2: ^7.27; H, 3.43; N, 7.95. Found: C, 47.89; H, 3.60; N, 7.94.;30;35;- 19 -;205191;WHAT /WE CLAIM IS:;1. An animal feed composition supplemented by a quantity of a compound of the formula:;S-S-R;in which:;R and R1 are, each, hydrogen, C^_g-alkyl,;C^^-alkoxy, C^_g-alkylthio, nitro, hydroxy, halo,;carboxy, phenyl, benzyl or benzylthio; and;2;R is a phenyl, benzyl, pyridyl, 1-oxo-pyridyl, each of the former has one or two optional substituents as defined for R and R1 above,;Ci_i2~alkyl» furyl, thienyl, glucosyl, alanyl, C^^-alkenyl or carboxy-C^_g-alkyl; a nontoxic,;stable salt thereof; or a latentiated derivative thereof; which is effective for increasing the growth rate and feed efficiency of a meat producing monogastric animal but which is nontoxic to said animal.;2. The composition of claim 1 in which quantity of the compound is selected from the range of from 1-100 parts of compound per million parts of composition by weight.;3. The composition of claim 1 in which the compound is a carboxyphenyl (l-oxo-2-pyridyl) disulfide.;4. The composition of claim 1 in which the compound is 2-carboxyphenyl (l-oxo-2-pyridyl) disulfide which is present in from 15-25 parts by weight of compound per million parts by weight of composition.;5. The composition of claim 1 in which the compound is 2-carboxyethyl (l-oxo-2-pyridyl) disulfide.;- 20 -;205291;6. A compound of the formula:;+;0;in which:;R and R^" are, each, hydrogen, C^_g-alkyl, C^_g-alkoxy, C^g-alkylthio, nitro, hydroxy, halo, carboxy, phenyl, benzyl or benzylthio? and;P is alkylene of 1-6 carbons or phenylene, or a nontoxic salt thereof.;7/ The compound of claim 6 being 2-carboxy-phenyl (l-oxo-2-pyridyl) disulfide.;8. The compound of claim 6 being 2-carboxy-ethyl (l-oxo-2-pyridyl) disulfide.;9. The method of improving weight gain and feed efficiency in animals comprising feeding a compound or composition of claims 1-8.;10. A. composition as claimed in any one of claims;2 to 5 substantially as hereinbefore described with reference to any example thereto.;11. A compound as claimed in any one of claims 6;to 8 substantially as hereinbefore described with, reference to any example thereto.;12. A, method as claimed in claim 9 when performed substantially as hereinbefore described.;BATED TH18'SisVI>AY 0Oc.W l&'SS" A, J. PARK & SON;AGENTS FOR THE APPLICANTS;*/ ,Us - r-iKDVS®7 V.,vV