NO971069L - Acetohydroxy acid synthase promoter expression of introduced genes in plants - Google Patents
Acetohydroxy acid synthase promoter expression of introduced genes in plantsInfo
- Publication number
- NO971069L NO971069L NO971069A NO971069A NO971069L NO 971069 L NO971069 L NO 971069L NO 971069 A NO971069 A NO 971069A NO 971069 A NO971069 A NO 971069A NO 971069 L NO971069 L NO 971069L
- Authority
- NO
- Norway
- Prior art keywords
- genes
- plants
- promoters
- maize
- promoter
- Prior art date
Links
- 108090000623 proteins and genes Proteins 0.000 title abstract 4
- 230000014509 gene expression Effects 0.000 title abstract 2
- 108010000700 Acetolactate synthase Proteins 0.000 title 1
- 241000196324 Embryophyta Species 0.000 abstract 3
- 240000008042 Zea mays Species 0.000 abstract 3
- 235000016383 Zea mays subsp huehuetenangensis Nutrition 0.000 abstract 3
- 235000002017 Zea mays subsp mays Nutrition 0.000 abstract 3
- 239000012634 fragment Substances 0.000 abstract 3
- 235000009973 maize Nutrition 0.000 abstract 3
- 108091028043 Nucleic acid sequence Proteins 0.000 abstract 2
- 108700039691 Genetic Promoter Regions Proteins 0.000 abstract 1
- 102000053187 Glucuronidase Human genes 0.000 abstract 1
- 108010060309 Glucuronidase Proteins 0.000 abstract 1
- 108700008625 Reporter Genes Proteins 0.000 abstract 1
- 239000013612 plasmid Substances 0.000 abstract 1
- 239000013598 vector Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
- C12N15/8209—Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
- C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/88—Lyases (4.)
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Biotechnology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Cell Biology (AREA)
- Medicinal Chemistry (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Saccharide Compounds (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
- Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The present invention is directed to isolated non-coding nucleotide sequences useful as promoters for heterologous gene expression in plants. The present invention is also directed to vectors and plant cells comprising the isolated nucleotide sequences. AHAS promoters from maize are used to express introduced genes at high levels and in various plant tissues. Promoters from the als1 and als2 genes of maize are cloned and sequenced, and the promoter regions from these genes are then introduced into a plasmid 5' to the reporter gene beta-glucuronidase (GUS). Both promoter fragments are from the XI12 maize line. The als1 promoter fragment is approximately 1400 base pairs long, whereas the als2 promoter fragment contains 829 base pairs.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US08/303,266 US5750866A (en) | 1994-09-08 | 1994-09-08 | AHAS promoter useful for expression of introduced genes in plants |
| PCT/US1995/011199 WO1996007746A1 (en) | 1994-09-08 | 1995-09-05 | Acetohydroxyacid synthase promoter expression of introduced genes in plants |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| NO971069D0 NO971069D0 (en) | 1997-03-07 |
| NO971069L true NO971069L (en) | 1997-05-07 |
| NO323171B1 NO323171B1 (en) | 2007-01-15 |
Family
ID=23171273
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| NO19971069A NO323171B1 (en) | 1994-09-08 | 1997-03-07 | Isolated nucleotide sequence comprising an acetohydroxy acid synthase promoter, use thereof, transformation vector, plant cell and plant comprising said nucleotide sequence, nucleic acid construction comprising the sequence and methods of uses thereof. |
Country Status (26)
| Country | Link |
|---|---|
| US (2) | US5750866A (en) |
| EP (1) | EP0779928B1 (en) |
| JP (1) | JP3655922B2 (en) |
| KR (1) | KR970705637A (en) |
| CN (1) | CN1210404C (en) |
| AT (1) | ATE291631T1 (en) |
| AU (1) | AU701239B2 (en) |
| BG (1) | BG64062B1 (en) |
| BR (1) | BR9509202A (en) |
| CA (1) | CA2198497C (en) |
| CZ (1) | CZ69597A3 (en) |
| DE (1) | DE69534101T2 (en) |
| EE (1) | EE03933B1 (en) |
| ES (1) | ES2240978T3 (en) |
| FI (1) | FI119818B (en) |
| HU (1) | HU221795B1 (en) |
| MD (1) | MD2312B2 (en) |
| MX (1) | MX9701684A (en) |
| NO (1) | NO323171B1 (en) |
| NZ (1) | NZ293093A (en) |
| PL (1) | PL188062B1 (en) |
| RO (1) | RO117190B1 (en) |
| RU (1) | RU2197527C2 (en) |
| SK (1) | SK30697A3 (en) |
| UA (1) | UA48951C2 (en) |
| WO (1) | WO1996007746A1 (en) |
Families Citing this family (56)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5659026A (en) * | 1995-03-24 | 1997-08-19 | Pioneer Hi-Bred International | ALS3 promoter |
| DE69932868T2 (en) | 1998-02-26 | 2007-03-15 | Pioneer Hi-Bred International, Inc. | Nucleic acid molecule having a nucleotide sequence for a promoter |
| EP1464707B1 (en) * | 1998-02-26 | 2006-10-18 | Pioneer Hi-Bred International, Inc. | Maize alpha-tubulin 3-18 promoter |
| US6504083B1 (en) * | 1998-10-06 | 2003-01-07 | Pioneer Hi-Bred International, Inc. | Maize Gos-2 promoters |
| US6528701B1 (en) | 1999-03-02 | 2003-03-04 | Board Of Supervisors Of Louisiana State University And Agricultural And Mechanical College | Rice ubiquitin-derived promoters |
| US7122721B1 (en) | 1999-10-05 | 2006-10-17 | Basf Aktiengesellschaft | Plant gene expression under the control of constitutive plant V-ATPase promoters |
| EP1284597A4 (en) | 2000-04-28 | 2005-12-28 | Basf Ag | Use of the maize x112 mutant ahas 2 gene and imidazolinone herbicides for selection of transgenic monocots, maize, rice and wheat plants resistant to the imidazolinone herbicides |
| AU2003249939A1 (en) | 2002-07-09 | 2004-01-23 | Basf Plant Science Gmbh | Use of ahas mutant genes as selection marker in potato transformation |
| EP2278018A3 (en) | 2002-08-07 | 2012-01-04 | BASF Plant Science GmbH | Nucleic acid sequences encoding proteins associated with abiotic stress response |
| CA2516042A1 (en) | 2003-02-17 | 2004-09-02 | Metanomics Gmbh | Preparation of transgenic plants comprising a nucleic acid molecule encoding an l450 polypeptide-exhibiting faster growth and/or increased yield |
| WO2005014828A2 (en) | 2003-08-01 | 2005-02-17 | Basf Plant Science Gmbh | Process for the production of fine chemicals in plants |
| CN103131673A (en) | 2003-04-15 | 2013-06-05 | 巴斯福植物科学有限公司 | Nucleic acid sequences encoding proteins associated with abiotic stress response and plant cells and plants with increased tolerance to environmental stress |
| EP1491634A1 (en) * | 2003-06-26 | 2004-12-29 | Degussa AG | Feedback resistant acetohydroxy acid synthetase mutants |
| CA2559760A1 (en) | 2004-07-02 | 2006-07-06 | Metanomics Gmbh | Process for the production of fine chemicals |
| AU2005268943B2 (en) | 2004-07-31 | 2011-04-14 | Metanomics Gmbh | Preparation of organisms with faster growth and/or higher yield |
| AU2005286427B2 (en) | 2004-09-24 | 2011-09-15 | Basf Plant Science Gmbh | Plant cells and plants with increased tolerance to environmental stress |
| CN103289961A (en) | 2004-09-24 | 2013-09-11 | 巴斯福植物科学有限公司 | Nucleic acid sequences encoding proteins associated with abiotic stress response and plant cells and plants with increased tolerance to environmental stress |
| CA2585798A1 (en) | 2004-12-17 | 2006-06-17 | Metanomics Gmbh | Process for the control of production of fine chemicals |
| WO2006092449A2 (en) * | 2005-03-02 | 2006-09-08 | Metanomics Gmbh | Process for the production of fine chemicals |
| EP2573188A2 (en) | 2005-03-02 | 2013-03-27 | Metanomics GmbH | Process for the production of fine chemicals |
| US7737326B2 (en) * | 2005-04-29 | 2010-06-15 | Midwest Oil Seeds Inc. | EPSPS promoter from maize |
| EP2221382A3 (en) | 2006-03-24 | 2010-12-01 | BASF Plant Science GmbH | Proteins associated with abiotic stress response and homologs |
| WO2008034648A1 (en) | 2006-04-05 | 2008-03-27 | Metanomics Gmbh | Process for the production of a fine chemical |
| BRPI0713097A2 (en) | 2006-05-31 | 2012-10-16 | Metanomics Gmbh | process for enhanced nitrogen uptake, accumulation and / or utilization and / or increased total nitrogen content in an active photosynthetic organism, isolated nucleic acid molecule, nucleic acid construction, vector, host cell, process for producing a polypeptide , polypeptide, antibody, plant tissue, propagating material, harvested material or a plant, method for the identification of a gene product, composition, and, use of nucleic acid molecule, polypeptide, nucleic acid construction, vector, plant or plant tissue, host cell material or gene product identified according to |
| WO2008043849A2 (en) | 2006-10-13 | 2008-04-17 | Basf Plant Science Gmbh | Plants with increased yield |
| US20080176225A1 (en) * | 2007-01-18 | 2008-07-24 | Steve Roffler | Membrane bound reporter gene system |
| NZ598210A (en) | 2007-04-04 | 2013-08-30 | Basf Plant Science Gmbh | AHAS mutants |
| EP2064330A2 (en) | 2007-05-22 | 2009-06-03 | BASF Plant Science GmbH | Plants with increased tolerance and/or resistance to environmental stress and increased biomass production |
| AR066642A1 (en) | 2007-05-22 | 2009-09-02 | Basf Plant Gmbh | VEGETABLE CELLS AND PLANTS WITH HIGHER TOLERANCE AND / OR RESISTANCE TO ENVIRONMENTAL STRESS AND HIGHER PRODUCTION OF BIOMASS PRODUCED BY SILENCING OF GENES |
| CN103695459A (en) | 2007-09-18 | 2014-04-02 | 巴斯夫植物科学有限公司 | Plants with increased yield |
| US8809059B2 (en) | 2007-09-21 | 2014-08-19 | Basf Plant Science Gmbh | Plants with increased yield |
| WO2009077611A2 (en) | 2007-12-19 | 2009-06-25 | Basf Plant Science Gmbh | Plants with increased yield and/or increased tolerance to environmental stress (iy-bm) |
| DE112008003433T5 (en) | 2007-12-21 | 2010-11-04 | Basf Plant Science Gmbh | Plants with increased yield (KO NUE) |
| US20110010800A1 (en) * | 2008-02-27 | 2011-01-13 | Basf Plant Science Gmbh | Plants with increased yield |
| MX2011001899A (en) | 2008-08-19 | 2011-04-05 | Basf Plant Science Gmbh | Plants with increased yield by increasing or generating one or more activities in a plant or a part thereof. |
| EP2337853A1 (en) | 2008-09-23 | 2011-06-29 | BASF Plant Science Company GmbH | Plants with increased yield (lt) |
| EP2350289A1 (en) | 2008-10-23 | 2011-08-03 | BASF Plant Science GmbH | Plants with increased yield (nue) |
| BRPI0924536A2 (en) | 2008-10-23 | 2015-08-11 | Basf Plant Science Gmbh | Method for producing a transgenic cell with increased gamma-aminobutyric acid (gaba) content to increase yield, isolated nucleic acid molecule, nucleic acid construct, vector, host cell, process for producing a polypeptide, polypeptide, antibody, and , cell nucleus, cell, plant cell nucleus, plant cell, plant tissue, propagation material, pollen, progeny, material collected or a plant |
| CN101475941B (en) * | 2008-11-28 | 2011-09-07 | 北京市农林科学院 | Maize Zmptil gene promoter and use thereof |
| WO2011009801A1 (en) | 2009-07-23 | 2011-01-27 | Basf Plant Science Company Gmbh | Plants with increased yield |
| SG178389A1 (en) | 2009-08-31 | 2012-03-29 | Basf Plant Science Co Gmbh | Regulatory nucleic acid molecules for enhancing constitutive gene expression in plants |
| AU2010288760B2 (en) | 2009-08-31 | 2016-01-21 | Basf Plant Science Company Gmbh | Regulatory nucleic acid molecules for enhancing seed-specific and/or seed-preferential gene expression in plants |
| EP3178937B1 (en) | 2009-08-31 | 2018-11-07 | BASF Plant Science Company GmbH | Regulatory nucleic acid molecules for enhancing seed-specific gene expression in plants promoting enhanced polyunsaturated fatty acid synthesis |
| EP2319872A1 (en) | 2009-11-04 | 2011-05-11 | BASF Plant Science GmbH | Amylopectin type starch with enhanced retrogradation stability |
| AU2010320547B2 (en) | 2009-11-17 | 2016-06-09 | Basf Plant Science Company Gmbh | Plants with increased yield |
| US9422569B2 (en) * | 2011-12-30 | 2016-08-23 | Dow Agrosciences Llc | Construct and method for synthetic bidirectional plant promoter UBI1 |
| CA2855125C (en) * | 2011-12-30 | 2021-03-09 | Dow Agrosciences Llc | Method and construct for synthetic bidirectional scbv plant promoter |
| CN102676549B (en) * | 2012-01-09 | 2014-04-09 | 中国中医科学院中药研究所 | CYP450 (Cytochrome P450) gene participating in tanshinone biosynthesis and coded product as well as application thereof |
| AU2013251382A1 (en) * | 2012-04-27 | 2014-10-16 | Synthetic Genomics, Inc. | Compositions and methods for modulating the sensitivity of cells to AHAS inhibitors |
| WO2014117990A1 (en) | 2013-01-29 | 2014-08-07 | Basf Plant Science Company Gmbh | Fungal resistant plants expressing hcp6 |
| CA2891424A1 (en) | 2013-01-29 | 2014-08-07 | Basf Plant Science Company Gmbh | Fungal resistant plants expressing ein2 |
| WO2014117988A1 (en) | 2013-01-29 | 2014-08-07 | Basf Plant Science Company Gmbh | Fungal resistant plants expressing hcp7 |
| WO2014135682A1 (en) | 2013-03-08 | 2014-09-12 | Basf Plant Science Company Gmbh | Fungal resistant plants expressing mybtf |
| US9617553B2 (en) | 2013-03-14 | 2017-04-11 | Monsanto Technology Llc | Plant regulatory elements and uses thereof |
| WO2016124515A1 (en) | 2015-02-04 | 2016-08-11 | Basf Plant Science Company Gmbh | Method of increasing resistance against soybean rust in transgenic plants by increasing the scopoletin content |
| EP3054014A3 (en) | 2016-05-10 | 2016-11-23 | BASF Plant Science Company GmbH | Use of a fungicide on transgenic plants |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4943674A (en) * | 1987-05-26 | 1990-07-24 | Calgene, Inc. | Fruit specific transcriptional factors |
| US5013659A (en) * | 1987-07-27 | 1991-05-07 | E. I. Du Pont De Nemours And Company | Nucleic acid fragment encoding herbicide resistant plant acetolactate synthase |
| EP0360750A3 (en) * | 1988-09-22 | 1991-01-02 | Ciba-Geigy Ag | Novel herbicide tolerant plants |
| EP0461355B1 (en) * | 1990-05-09 | 1997-06-11 | American Cyanamid Company | A method for the prevention of crop injury in the presence of synergistic pesticide combinations |
| GB9024728D0 (en) * | 1990-11-14 | 1991-01-02 | Ici Plc | Herbicide resistant plants |
| TW208716B (en) * | 1990-12-27 | 1993-07-01 | American Cyanamid Co | |
| US5731180A (en) * | 1991-07-31 | 1998-03-24 | American Cyanamid Company | Imidazolinone resistant AHAS mutants |
-
1994
- 1994-09-08 US US08/303,266 patent/US5750866A/en not_active Expired - Lifetime
-
1995
- 1995-09-05 UA UA97041679A patent/UA48951C2/en unknown
- 1995-09-05 AT AT95932390T patent/ATE291631T1/en active
- 1995-09-05 RO RO97-00422A patent/RO117190B1/en unknown
- 1995-09-05 KR KR1019970701508A patent/KR970705637A/en not_active Ceased
- 1995-09-05 PL PL95319053A patent/PL188062B1/en unknown
- 1995-09-05 WO PCT/US1995/011199 patent/WO1996007746A1/en not_active Ceased
- 1995-09-05 CZ CZ97695A patent/CZ69597A3/en unknown
- 1995-09-05 EP EP95932390A patent/EP0779928B1/en not_active Expired - Lifetime
- 1995-09-05 RU RU97106101/13A patent/RU2197527C2/en active
- 1995-09-05 NZ NZ293093A patent/NZ293093A/en unknown
- 1995-09-05 DE DE69534101T patent/DE69534101T2/en not_active Expired - Lifetime
- 1995-09-05 MX MX9701684A patent/MX9701684A/en active IP Right Grant
- 1995-09-05 BR BR9509202A patent/BR9509202A/en not_active IP Right Cessation
- 1995-09-05 MD MD97-0144A patent/MD2312B2/en unknown
- 1995-09-05 CA CA002198497A patent/CA2198497C/en not_active Expired - Lifetime
- 1995-09-05 CN CNB951949802A patent/CN1210404C/en not_active Expired - Lifetime
- 1995-09-05 AU AU35448/95A patent/AU701239B2/en not_active Expired
- 1995-09-05 EE EE9700213A patent/EE03933B1/en not_active IP Right Cessation
- 1995-09-05 JP JP50961796A patent/JP3655922B2/en not_active Expired - Lifetime
- 1995-09-05 SK SK306-97A patent/SK30697A3/en unknown
- 1995-09-05 ES ES95932390T patent/ES2240978T3/en not_active Expired - Lifetime
- 1995-09-05 HU HU9701927A patent/HU221795B1/en active IP Right Grant
-
1997
- 1997-02-27 BG BG101270A patent/BG64062B1/en unknown
- 1997-03-06 FI FI970958A patent/FI119818B/en not_active IP Right Cessation
- 1997-03-07 NO NO19971069A patent/NO323171B1/en not_active IP Right Cessation
- 1997-05-23 US US08/862,881 patent/US6025541A/en not_active Expired - Lifetime
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| MK1K | Patent expired |