NO954935L - Væskeströmcytometer - Google Patents
VæskeströmcytometerInfo
- Publication number
- NO954935L NO954935L NO954935A NO954935A NO954935L NO 954935 L NO954935 L NO 954935L NO 954935 A NO954935 A NO 954935A NO 954935 A NO954935 A NO 954935A NO 954935 L NO954935 L NO 954935L
- Authority
- NO
- Norway
- Prior art keywords
- particles
- measurement zone
- dichroic mirror
- zone
- radiation path
- Prior art date
Links
Landscapes
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
Et vaeskestrømcytometer til telling og klassifikasjon av partikler så som biologiske celler eller andre mi- kroskopiske partikler i væsker omfatter et optisk system for belysning og deteksjon i en målesone (7), slik at optisk dispersjon og fluorescens bevirket av partiklene samtidig kan detekteres når partiklene passerer målesonen. Det optiske system omfatter i en første strålevei (S,) en speilreflektor (9) med brenn- punkt i målesonen (7), en kvartbølgeplate (8) mellom målesonen (7) og speilreflektoren (9), en linse (6) med brennpunkt i målesonen (7) og anordnet mellom målesonen (7) og et dikroisk speil (5), idet det dikroiske speil (5) er anordnet slik at det gir reflek- sjon til en annen strålevei (82). I den annen strålevei (52) er en stråledeler (4) anordnet mellom det dikroiske speil (9) og en lyskilde (1) og frembringer en tredje strålevei (S3). Cytometeret omfatter en første detektor (13) anordnet i den første strålevei (S,) etter det dikroiske speil (5) og innrettet til deteksjon av fluorescens avgitt av partiklene i målesonen (7), samt en annen detektor (16) anordnet i den tredje strålevei (53) og innrettet til deteksjon av optisk dispersjon forårsaket av partiklene i målesonen (7).A liquid flow cytometer for counting and classifying particles such as biological cells or other microscopic particles in liquids comprises an optical system for illumination and detection in a measurement zone (7), so that optical dispersion and fluorescence effected by the particles can be simultaneously detected as the particles pass the measurement zone. The optical system comprises, in a first radiation path (S,) a mirror reflector (9) with a focal point in the measurement zone (7), a quarter-wave plate (8) between the measurement zone (7) and the mirror reflector (9), a lens (6) with a focal point. in the measuring zone (7) and arranged between the measuring zone (7) and a dichroic mirror (5), the dichroic mirror (5) being arranged so as to give reflection to another radiation path (82). In the second beam path (52), a beam splitter (4) is arranged between the dichroic mirror (9) and a light source (1) and produces a third beam path (S3). The cytometer comprises a first detector (13) arranged in the first radiation path (S S) following the dichroic mirror (5) and arranged for the detection of fluorescence emitted by the particles in the measurement zone (7), and a second detector (16) arranged in the third radiation path (53) and arranged for detection of optical dispersion caused by the particles in the measurement zone (7).
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
NO954935A NO954935L (en) | 1993-06-08 | 1995-12-06 | Væskeströmcytometer |
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
NO932088A NO932088L (en) | 1993-06-08 | 1993-06-08 | Device for use in liquid flow cytometry |
PCT/NO1994/000106 WO1994029695A1 (en) | 1993-06-08 | 1994-06-08 | Liquid flow cytometer |
NO954935A NO954935L (en) | 1993-06-08 | 1995-12-06 | Væskeströmcytometer |
Publications (2)
Publication Number | Publication Date |
---|---|
NO954935D0 NO954935D0 (en) | 1995-12-06 |
NO954935L true NO954935L (en) | 1995-12-13 |
Family
ID=26648417
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
NO954935A NO954935L (en) | 1993-06-08 | 1995-12-06 | Væskeströmcytometer |
Country Status (1)
Country | Link |
---|---|
NO (1) | NO954935L (en) |
-
1995
- 1995-12-06 NO NO954935A patent/NO954935L/en not_active Application Discontinuation
Also Published As
Publication number | Publication date |
---|---|
NO954935D0 (en) | 1995-12-06 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
FC2A | Withdrawal, rejection or dismissal of laid open patent application |