NO954935L - Væskeströmcytometer - Google Patents

Væskeströmcytometer

Info

Publication number
NO954935L
NO954935L NO954935A NO954935A NO954935L NO 954935 L NO954935 L NO 954935L NO 954935 A NO954935 A NO 954935A NO 954935 A NO954935 A NO 954935A NO 954935 L NO954935 L NO 954935L
Authority
NO
Norway
Prior art keywords
particles
measurement zone
dichroic mirror
zone
radiation path
Prior art date
Application number
NO954935A
Other languages
Norwegian (no)
Other versions
NO954935D0 (en
Inventor
Oddbjoern Gjelsnes
Reidar Tangen
Original Assignee
Oddbjoern Gjelsnes
Reidar Tangen
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from NO932088A external-priority patent/NO932088L/en
Application filed by Oddbjoern Gjelsnes, Reidar Tangen filed Critical Oddbjoern Gjelsnes
Priority to NO954935A priority Critical patent/NO954935L/en
Publication of NO954935D0 publication Critical patent/NO954935D0/en
Publication of NO954935L publication Critical patent/NO954935L/en

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  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

Et vaeskestrømcytometer til telling og klassifikasjon av partikler så som biologiske celler eller andre mi- kroskopiske partikler i væsker omfatter et optisk system for belysning og deteksjon i en målesone (7), slik at optisk dispersjon og fluorescens bevirket av partiklene samtidig kan detekteres når partiklene passerer målesonen. Det optiske system omfatter i en første strålevei (S,) en speilreflektor (9) med brenn- punkt i målesonen (7), en kvartbølgeplate (8) mellom målesonen (7) og speilreflektoren (9), en linse (6) med brennpunkt i målesonen (7) og anordnet mellom målesonen (7) og et dikroisk speil (5), idet det dikroiske speil (5) er anordnet slik at det gir reflek- sjon til en annen strålevei (82). I den annen strålevei (52) er en stråledeler (4) anordnet mellom det dikroiske speil (9) og en lyskilde (1) og frembringer en tredje strålevei (S3). Cytometeret omfatter en første detektor (13) anordnet i den første strålevei (S,) etter det dikroiske speil (5) og innrettet til deteksjon av fluorescens avgitt av partiklene i målesonen (7), samt en annen detektor (16) anordnet i den tredje strålevei (53) og innrettet til deteksjon av optisk dispersjon forårsaket av partiklene i målesonen (7).A liquid flow cytometer for counting and classifying particles such as biological cells or other microscopic particles in liquids comprises an optical system for illumination and detection in a measurement zone (7), so that optical dispersion and fluorescence effected by the particles can be simultaneously detected as the particles pass the measurement zone. The optical system comprises, in a first radiation path (S,) a mirror reflector (9) with a focal point in the measurement zone (7), a quarter-wave plate (8) between the measurement zone (7) and the mirror reflector (9), a lens (6) with a focal point. in the measuring zone (7) and arranged between the measuring zone (7) and a dichroic mirror (5), the dichroic mirror (5) being arranged so as to give reflection to another radiation path (82). In the second beam path (52), a beam splitter (4) is arranged between the dichroic mirror (9) and a light source (1) and produces a third beam path (S3). The cytometer comprises a first detector (13) arranged in the first radiation path (S S) following the dichroic mirror (5) and arranged for the detection of fluorescence emitted by the particles in the measurement zone (7), and a second detector (16) arranged in the third radiation path (53) and arranged for detection of optical dispersion caused by the particles in the measurement zone (7).

NO954935A 1993-06-08 1995-12-06 Væskeströmcytometer NO954935L (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
NO954935A NO954935L (en) 1993-06-08 1995-12-06 Væskeströmcytometer

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
NO932088A NO932088L (en) 1993-06-08 1993-06-08 Device for use in liquid flow cytometry
PCT/NO1994/000106 WO1994029695A1 (en) 1993-06-08 1994-06-08 Liquid flow cytometer
NO954935A NO954935L (en) 1993-06-08 1995-12-06 Væskeströmcytometer

Publications (2)

Publication Number Publication Date
NO954935D0 NO954935D0 (en) 1995-12-06
NO954935L true NO954935L (en) 1995-12-13

Family

ID=26648417

Family Applications (1)

Application Number Title Priority Date Filing Date
NO954935A NO954935L (en) 1993-06-08 1995-12-06 Væskeströmcytometer

Country Status (1)

Country Link
NO (1) NO954935L (en)

Also Published As

Publication number Publication date
NO954935D0 (en) 1995-12-06

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