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Priority claimed from US07/956,697external-prioritypatent/US5374543A/en
Application filed by Amgen IncfiledCriticalAmgen Inc
Publication of NO951241D0publicationCriticalpatent/NO951241D0/en
Publication of NO951241LpublicationCriticalpatent/NO951241L/en
DNA-molekyler som koder for en modifisert tryptofansyntetase-p-suben-het. Når de uttrykkes i en rekombinant vertsmikroorganisme muliggjør disse polypeptidanaloger produksjon og akkumulering av betydelige mengder intracellulært indol. I nærvær av et aromatisk dioksygenaseenzym kan det således produserte indol omdannes til indoksyl som ved innvirkning av luft oksiderer til indigo.DNA molecules encoding a modified tryptophan synthetase β subunit. When expressed in a recombinant host microorganism, these polypeptide analogs enable the production and accumulation of significant amounts of intracellular indole. In the presence of an aromatic dioxygenase enzyme, the indole thus produced can be converted to indoxyl which, under the action of air, oxidizes to indigo.
A single cyclohexadienyl dehydrogenase specifies the prephenate dehydrogenase and arogenate dehydrogenase components of the dual pathways to L-tyrosine in Pseudomonas aeruginosa.