NO950918L - Method for Detecting Specific Target Cells in Specialized or Mixed Cell Populations and Solutions Containing Mixed Cell Population - Google Patents

Method for Detecting Specific Target Cells in Specialized or Mixed Cell Populations and Solutions Containing Mixed Cell Population

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Publication number
NO950918L
NO950918L NO950918A NO950918A NO950918L NO 950918 L NO950918 L NO 950918L NO 950918 A NO950918 A NO 950918A NO 950918 A NO950918 A NO 950918A NO 950918 L NO950918 L NO 950918L
Authority
NO
Norway
Prior art keywords
mixed cell
target cells
antibodies
detecting specific
specialized
Prior art date
Application number
NO950918A
Other languages
Norwegian (no)
Other versions
NO950918D0 (en
Inventor
Oeystein Fodstad
Gunnar Kvalheim
Original Assignee
Oeystein Fodstad
Gunnar Kvalheim
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from PCT/NO1992/000151 external-priority patent/WO1994007138A1/en
Application filed by Oeystein Fodstad, Gunnar Kvalheim filed Critical Oeystein Fodstad
Priority to NO950918A priority Critical patent/NO950918L/en
Publication of NO950918D0 publication Critical patent/NO950918D0/en
Publication of NO950918L publication Critical patent/NO950918L/en

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Abstract

Oppfinnelsen angår en fremgangsmåte til å detektere spesifikke målceller på en enkel og tids- besparende måte, ved å anvende paramagnetiske partikler, antistoffer som gjenkjenner Fc-delen av målcelleassosierende antistoffer og målcelle- assosierende rettet på spesifikke antigendeter- minanter i målcellemembranene. Inkubasjon av cellesuspensjonen med en mild detergent og/eller et andre sett av antistoffer eller antistoffragmenter, formerket eller ikke med fluorescerende midler, metallokolloider, radioisotoper, biotin-komplekser eller visse enzymer som tillater visualisering vil dramatisk øke spesifisiteten til fremgangsmåten. Fremgangsmåten kan videre anvendes for isolering av målceller ved magnetisk feltapplikasjon og et sett for å utføre fremgangsmåten i henhold til opp- finnelsen er beskrevet.The invention relates to a method for detecting specific target cells in a simple and time-saving manner, using paramagnetic particles, antibodies which recognize the Fc portion of target cell associating antibodies and target cell associates targeted to specific antigenic determinants in the target cell membranes. Incubation of the cell suspension with a mild detergent and / or a second set of antibodies or antibody fragments, labeled or not with fluorescent agents, metallocolloids, radioisotopes, biotin complexes or certain enzymes that allow visualization will dramatically increase the specificity of the method. The method can further be used for isolating target cells by magnetic field application and a kit for carrying out the method according to the invention is described.

NO950918A 1992-09-14 1995-03-10 Method for Detecting Specific Target Cells in Specialized or Mixed Cell Populations and Solutions Containing Mixed Cell Population NO950918L (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
NO950918A NO950918L (en) 1992-09-14 1995-03-10 Method for Detecting Specific Target Cells in Specialized or Mixed Cell Populations and Solutions Containing Mixed Cell Population

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
PCT/NO1992/000151 WO1994007138A1 (en) 1992-09-14 1992-09-14 Detection of specific target cells in specialized or mixed cell population and solutions containing mixed cell populations
PCT/NO1993/000136 WO1994007139A1 (en) 1992-09-14 1993-09-10 Improved method for detection of specific target cells in specialized or mixed cell population and solutions containing mixed cell populations
NO950918A NO950918L (en) 1992-09-14 1995-03-10 Method for Detecting Specific Target Cells in Specialized or Mixed Cell Populations and Solutions Containing Mixed Cell Population

Publications (2)

Publication Number Publication Date
NO950918D0 NO950918D0 (en) 1995-03-10
NO950918L true NO950918L (en) 1995-05-10

Family

ID=34797878

Family Applications (1)

Application Number Title Priority Date Filing Date
NO950918A NO950918L (en) 1992-09-14 1995-03-10 Method for Detecting Specific Target Cells in Specialized or Mixed Cell Populations and Solutions Containing Mixed Cell Population

Country Status (1)

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NO (1) NO950918L (en)

Also Published As

Publication number Publication date
NO950918D0 (en) 1995-03-10

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