NO950918L - Method for Detecting Specific Target Cells in Specialized or Mixed Cell Populations and Solutions Containing Mixed Cell Population - Google Patents
Method for Detecting Specific Target Cells in Specialized or Mixed Cell Populations and Solutions Containing Mixed Cell PopulationInfo
- Publication number
- NO950918L NO950918L NO950918A NO950918A NO950918L NO 950918 L NO950918 L NO 950918L NO 950918 A NO950918 A NO 950918A NO 950918 A NO950918 A NO 950918A NO 950918 L NO950918 L NO 950918L
- Authority
- NO
- Norway
- Prior art keywords
- mixed cell
- target cells
- antibodies
- detecting specific
- specialized
- Prior art date
Links
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- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Oppfinnelsen angår en fremgangsmåte til å detektere spesifikke målceller på en enkel og tids- besparende måte, ved å anvende paramagnetiske partikler, antistoffer som gjenkjenner Fc-delen av målcelleassosierende antistoffer og målcelle- assosierende rettet på spesifikke antigendeter- minanter i målcellemembranene. Inkubasjon av cellesuspensjonen med en mild detergent og/eller et andre sett av antistoffer eller antistoffragmenter, formerket eller ikke med fluorescerende midler, metallokolloider, radioisotoper, biotin-komplekser eller visse enzymer som tillater visualisering vil dramatisk øke spesifisiteten til fremgangsmåten. Fremgangsmåten kan videre anvendes for isolering av målceller ved magnetisk feltapplikasjon og et sett for å utføre fremgangsmåten i henhold til opp- finnelsen er beskrevet.The invention relates to a method for detecting specific target cells in a simple and time-saving manner, using paramagnetic particles, antibodies which recognize the Fc portion of target cell associating antibodies and target cell associates targeted to specific antigenic determinants in the target cell membranes. Incubation of the cell suspension with a mild detergent and / or a second set of antibodies or antibody fragments, labeled or not with fluorescent agents, metallocolloids, radioisotopes, biotin complexes or certain enzymes that allow visualization will dramatically increase the specificity of the method. The method can further be used for isolating target cells by magnetic field application and a kit for carrying out the method according to the invention is described.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
NO950918A NO950918L (en) | 1992-09-14 | 1995-03-10 | Method for Detecting Specific Target Cells in Specialized or Mixed Cell Populations and Solutions Containing Mixed Cell Population |
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
PCT/NO1992/000151 WO1994007138A1 (en) | 1992-09-14 | 1992-09-14 | Detection of specific target cells in specialized or mixed cell population and solutions containing mixed cell populations |
PCT/NO1993/000136 WO1994007139A1 (en) | 1992-09-14 | 1993-09-10 | Improved method for detection of specific target cells in specialized or mixed cell population and solutions containing mixed cell populations |
NO950918A NO950918L (en) | 1992-09-14 | 1995-03-10 | Method for Detecting Specific Target Cells in Specialized or Mixed Cell Populations and Solutions Containing Mixed Cell Population |
Publications (2)
Publication Number | Publication Date |
---|---|
NO950918D0 NO950918D0 (en) | 1995-03-10 |
NO950918L true NO950918L (en) | 1995-05-10 |
Family
ID=34797878
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
NO950918A NO950918L (en) | 1992-09-14 | 1995-03-10 | Method for Detecting Specific Target Cells in Specialized or Mixed Cell Populations and Solutions Containing Mixed Cell Population |
Country Status (1)
Country | Link |
---|---|
NO (1) | NO950918L (en) |
-
1995
- 1995-03-10 NO NO950918A patent/NO950918L/en not_active Application Discontinuation
Also Published As
Publication number | Publication date |
---|---|
NO950918D0 (en) | 1995-03-10 |
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Legal Events
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FC2A | Withdrawal, rejection or dismissal of laid open patent application |