NO934613L - Method for producing high-performance carotenoid-producing microorganisms, microorganisms obtained by the method and a process for producing carotenoid-containing cells or cellular components or purified carotenoid - Google Patents
Method for producing high-performance carotenoid-producing microorganisms, microorganisms obtained by the method and a process for producing carotenoid-containing cells or cellular components or purified carotenoidInfo
- Publication number
- NO934613L NO934613L NO934613A NO934613A NO934613L NO 934613 L NO934613 L NO 934613L NO 934613 A NO934613 A NO 934613A NO 934613 A NO934613 A NO 934613A NO 934613 L NO934613 L NO 934613L
- Authority
- NO
- Norway
- Prior art keywords
- carotenoid
- producing
- microorganisms
- cells
- grown
- Prior art date
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- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Høytytende, karotenoid- produserende, spesielt astaxanthin- produserende, mikroorganismer fremstil- les ved behandling av celler fra en stamme av karotenoidproduserende mikro- organismer med et mutagen, dyrkning av de mutageniserte celler i nærvær av en fytoendehydrogenaseinhibitor for å redusere nivået av karotenoidproduksjon hos alle cellene, og utvelgning av en mutant celle eller koloni som viser økt karotenoid farving når den sammenlignes med bakgrunnen. Den karotenoidproduser- ende mikroorganisme er særlig Phaffia rhodozyma, og cellene dyrkes til den sene stasjonære fase før de behandles med mutagenet. De fremstilte mikro- organismer kan dyrkes med henblikk på å oppnå en biomasse inneholdende karoten- oidet i en mengde på minst 1600, helst minst 1900 ug/g tørrvekt. Når en karo- tenoidproduserende mikroorganisme dyrkes med henblikk på å produsere karotenoidinneholdende, mikrobielle celler eller renset karotenoid, kan spesielt høye utbytter av karotenoid oppnås ved å dyrke cellene til den haploide fase før de høstes eller be- handles ytterligere. Enda høyere ut- bytter vil kunne oppnås ved å bruke et dyrkningsmedium inneholdende peroxisom- induserende stoffer.High-performance, carotenoid-producing, especially astaxanthin-producing, microorganisms are produced by treating cells from a strain of carotenoid-producing microorganisms with a mutagen, cultivating the mutagenized cells in the presence of a phytohydrogenase inhibitor to reduce the level of carotenoid production in all cells. , and selection of a mutant cell or colony that shows increased carotenoid staining when compared to the background. The carotenoid-producing microorganism is especially Phaffia rhodozyma, and the cells are grown to the late stationary phase before being treated with the mutagen. The microorganisms produced can be grown to obtain a biomass containing the carotenoid in an amount of at least 1600, preferably at least 1900 µg / g dry weight. When a carotenoid-producing microorganism is grown to produce carotenoid-containing, microbial cells or purified carotenoid, particularly high yields of carotenoid can be obtained by culturing the cells to the haploid phase before harvesting or further processing. Even higher yields can be obtained by using a culture medium containing peroxisome-inducing substances.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DK911151A DK115191D0 (en) | 1991-06-14 | 1991-06-14 | PROCEDURES FOR THE CREATION OF CAROTENOID PRODUCING, ISAAS ASTAXANTHIN PRODUCING, MICRO ORGANISMS, MICRO-ORGANISTS PROCESSED BY THE PROCEDURE AND PROCEDURES OF CAROTHENOID ORCEDICIDIC MICRO-ORENOID ORIGINOID ORIGINOID MICROCENE |
PCT/DK1992/000186 WO1992022648A1 (en) | 1991-06-14 | 1992-06-15 | A method of generating high carotenoid-producing microorganisms, microorganisms obtained by the method and a process for producing carotenoid-containing cells or cell parts or purified carotenoid |
Publications (2)
Publication Number | Publication Date |
---|---|
NO934613D0 NO934613D0 (en) | 1993-12-14 |
NO934613L true NO934613L (en) | 1994-02-14 |
Family
ID=26065298
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
NO934613A NO934613L (en) | 1991-06-14 | 1993-12-14 | Method for producing high-performance carotenoid-producing microorganisms, microorganisms obtained by the method and a process for producing carotenoid-containing cells or cellular components or purified carotenoid |
Country Status (1)
Country | Link |
---|---|
NO (1) | NO934613L (en) |
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1993
- 1993-12-14 NO NO934613A patent/NO934613L/en unknown
Also Published As
Publication number | Publication date |
---|---|
NO934613D0 (en) | 1993-12-14 |
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