NO920361L - Kolesterolesterase med variabel substratspesifisitet - Google Patents

Kolesterolesterase med variabel substratspesifisitet

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Publication number
NO920361L
NO920361L NO92920361A NO920361A NO920361L NO 920361 L NO920361 L NO 920361L NO 92920361 A NO92920361 A NO 92920361A NO 920361 A NO920361 A NO 920361A NO 920361 L NO920361 L NO 920361L
Authority
NO
Norway
Prior art keywords
cholesterol esterase
substrate specificity
variable substrate
amino acid
cholesterol
Prior art date
Application number
NO92920361A
Other languages
English (en)
Other versions
NO920361D0 (no
Inventor
Guenther Schumacher
Christian Hanke
Stephan Fischer
Original Assignee
Boehringer Mannheim Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from DE4018152A external-priority patent/DE4018152A1/de
Application filed by Boehringer Mannheim Gmbh filed Critical Boehringer Mannheim Gmbh
Publication of NO920361L publication Critical patent/NO920361L/no
Publication of NO920361D0 publication Critical patent/NO920361D0/no

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  • Enzymes And Modification Thereof (AREA)

Abstract

Kolesterolesteraser med variabel substratspesifisitet blir fremstilt ved kloning av et kolesterolesterasegen der det aktive sentret inneholder sentret -Gly-His-Ser-X-Gly, hvor X står for en aminosyre i en vektor, transformasjon av en mikroorgnaisme med denne vektoren og ekspresjon av kolesterolesterasegenet. Aminosyren X til det aktive sentret blir utvekslet med en annen aminosyre ved mutagenese.
NO920361A 1990-06-06 1992-01-27 Kolesterolesterase med variabel substratspesifisitet NO920361D0 (no)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE4018152A DE4018152A1 (de) 1990-06-06 1990-06-06 Cholesterinesterasen mit variabler substrat-spezifitaet
PCT/EP1991/001089 WO1991018987A1 (de) 1990-06-06 1991-06-06 Cholesterinesterasen mit variabler substrat-spezifität

Publications (2)

Publication Number Publication Date
NO920361L true NO920361L (no) 1992-01-27
NO920361D0 NO920361D0 (no) 1992-01-27

Family

ID=25893902

Family Applications (1)

Application Number Title Priority Date Filing Date
NO920361A NO920361D0 (no) 1990-06-06 1992-01-27 Kolesterolesterase med variabel substratspesifisitet

Country Status (1)

Country Link
NO (1) NO920361D0 (no)

Also Published As

Publication number Publication date
NO920361D0 (no) 1992-01-27

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