NO910787L - THREE-DIMENSIONAL CELL AND TISSUE CULTURE SYSTEM. - Google Patents

THREE-DIMENSIONAL CELL AND TISSUE CULTURE SYSTEM.

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Publication number
NO910787L
NO910787L NO91910787A NO910787A NO910787L NO 910787 L NO910787 L NO 910787L NO 91910787 A NO91910787 A NO 91910787A NO 910787 A NO910787 A NO 910787A NO 910787 L NO910787 L NO 910787L
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Norway
Prior art keywords
cells
dimensional
cultured
culture system
dimensional cell
Prior art date
Application number
NO91910787A
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Norwegian (no)
Other versions
NO910787D0 (en
Inventor
Gail K Naughton
Brian A Naughton
Original Assignee
Marrow Tech Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US07/242,096 external-priority patent/US4963489A/en
Priority claimed from US07/402,104 external-priority patent/US5032508A/en
Application filed by Marrow Tech Inc filed Critical Marrow Tech Inc
Publication of NO910787D0 publication Critical patent/NO910787D0/en
Publication of NO910787L publication Critical patent/NO910787L/en

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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0062—General methods for three-dimensional culture
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    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
    • A—HUMAN NECESSITIES
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    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
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  • Health & Medical Sciences (AREA)
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  • Biomedical Technology (AREA)
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  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Epidemiology (AREA)
  • Transplantation (AREA)
  • Oral & Maxillofacial Surgery (AREA)
  • Medicinal Chemistry (AREA)
  • Dermatology (AREA)
  • Neurology (AREA)
  • Neurosurgery (AREA)
  • Botany (AREA)
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  • Gastroenterology & Hepatology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Hematology (AREA)
  • Immunology (AREA)
  • Rheumatology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
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Abstract

Et tredimensjonalt cellekultursystem som Kan bil anvendt for å dyrke forskjellige celler og vev In vltro l forlengede tidsperioder er beskrevet. Celler avledet fra et ensket vev blir inokulert og dyrket på en på forhånd etablert stromabærermatrlse. Stromabærer- matrisen omfatter stromaceller, så som flbroblaster som aktivt vokser på en tredimensjonal matrise. Stroma- cellene kan også Innbefatte andre celler som finnes l løst bindevev så som endotellske celler, makrofager/ monocytter, adlpocytter, perlcytter, retlkulære celler som finnes l benmargsstroma osv. Stromamatrlsen tilvelebringer bæreren, vekstfaktorer og regulerende faktorer som er nødvendige for vedvarende langtlds aktiv prollferasjon av cellene l kultur. Når dyrket l dette tredimensjonale systemet, modner og segregerer de prollfererende cellene riktig for dannelsen av kom- ponenter til voksent vev analogt med motpartene som finnes In vlvo.A three-dimensional cell culture system that Kan car used to grow various cells and tissues In extended periods of time is described. Cells derived from an individual tissue are inoculated and cultured on a pre-established flow carrier matrix. The stromal carrier matrix includes stromal cells, such as flbroblasts that actively grow on a three-dimensional matrix. The stroma cells may also include other cells that are present in loose connective tissue such as endothelial cells, macrophages / monocytes, adipocytes, beads, reticular cells present in bone marrow stroma, etc. of the cells in culture. When cultured in this three-dimensional system, the proliferating cells mature and mature properly for the formation of adult tissue components analogous to the counterparts found in vlvo.

NO91910787A 1988-09-08 1991-02-27 THREE-DIMENSIONAL CELL AND TISSUE CULTURE SYSTEM. NO910787L (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US07/242,096 US4963489A (en) 1987-04-14 1988-09-08 Three-dimensional cell and tissue culture system
US07/402,104 US5032508A (en) 1988-09-08 1989-09-01 Three-dimensional cell and tissue culture system
PCT/US1989/003853 WO1990002796A1 (en) 1988-09-08 1989-09-07 Three-dimensional cell and tissue culture system

Publications (2)

Publication Number Publication Date
NO910787D0 NO910787D0 (en) 1991-02-27
NO910787L true NO910787L (en) 1991-04-22

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Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU621722B2 (en) * 1989-03-16 1992-03-19 W.R. Grace & Co.-Conn. Cell culture substrate, cell sheet, cell cluster and preparations thereof
AU665739B3 (en) * 1994-10-17 1996-01-11 Stegbar Pty Limited Improved window frame construction

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4485097A (en) * 1982-05-26 1984-11-27 Massachusetts Institute Of Technology Bone-equivalent and method for preparation thereof
HU202578B (en) * 1986-04-18 1991-03-28 Marrow Tech Inc Process for repricating bone marrow celles in three-dimension celle-cultivating systhem, three-dimension vehicle for the systhem and process for testing citotoxicity of the systhem
CA1340581C (en) * 1986-11-20 1999-06-08 Joseph P. Vacanti Chimeric neomorphogenesis of organs by controlled cellular implantation using artificial matrices

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AU644578B2 (en) 1993-12-16
NO910787D0 (en) 1991-02-27

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NO910787L (en) THREE-DIMENSIONAL CELL AND TISSUE CULTURE SYSTEM.