NO903788L - MONOCLONAL ANTIBODY AGAINST PROTEIN C. - Google Patents

MONOCLONAL ANTIBODY AGAINST PROTEIN C.

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Publication number
NO903788L
NO903788L NO90903788A NO903788A NO903788L NO 903788 L NO903788 L NO 903788L NO 90903788 A NO90903788 A NO 90903788A NO 903788 A NO903788 A NO 903788A NO 903788 L NO903788 L NO 903788L
Authority
NO
Norway
Prior art keywords
protein
antibody
activation
isolation
peptide
Prior art date
Application number
NO90903788A
Other languages
Norwegian (no)
Other versions
NO903788D0 (en
Inventor
Charles T Esmon
Naomi L Esmon
Original Assignee
Oklahoma Med Res Found
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from PCT/US1989/005851 external-priority patent/WO1990007524A1/en
Application filed by Oklahoma Med Res Found filed Critical Oklahoma Med Res Found
Publication of NO903788D0 publication Critical patent/NO903788D0/en
Publication of NO903788L publication Critical patent/NO903788L/en

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Abstract

Ca2+-avhengig monoklonalt antistoff som spesifikt binder til en spesifikk tolv-peptidsekvens (EDQVDPRLIDGK) i aktiveringsregionen i protein C. Antistoffet binder ikke til aktivert protein C og benyttes for å hemme aktivering av protein C ved trombin-trombomodulin. Antistoffet kan isoleres fra cellekultur eller ascitesvæske i store mengder ved affinitetskromatografi under milde betingelser ved bruk av peptidet bundet til et immobilisert substrat. Antistoffet har flere spesifikke anvendelser ved isolering og karakterisering av protein C og som en modell for konstruk- sjon av Ca2+-avhengig antistoffer for isoleringen av andre proteiner, som et diagnostikum, og som et terapeutikum for å hindre aktivering av protein C. Protein C kan produseres naturlig eller produseres ved ekspresjon av det rekombinante genet. Fordelene med antistoffet i rensingen av protein C inkluderer spesifisiteten for protein C og ikke for aktivert protein C, og den unike Ca2+-peptidbindingsspesifisiteten som tillater bindingssetet å være beskyttet når det er immobilisert på det kromatografiske underlag. In vivo har antistoffet blitt demonstrert å hemme tumorvekst. Antistoffet kan også benyttes for å fremme koagulasjon hos pasienter som har høye nivåer av faktor VTII-inhibitorer.Ca 2+ -dependent monoclonal antibody that specifically binds to a specific twelve-peptide sequence (EDQVDPRLIDGK) in the activation region of protein C. The antibody does not bind to activated protein C and is used to inhibit activation of protein C by thrombin thrombomodulin. The antibody can be isolated from cell culture or ascites fluid in large quantities by affinity chromatography under mild conditions using the peptide bound to an immobilized substrate. The antibody has several specific uses for the isolation and characterization of protein C and as a model for the construction of Ca 2+ -dependent antibodies for the isolation of other proteins, as a diagnostic agent, and as a therapeutic agent to prevent activation of protein C. Protein C produced naturally or produced by expression of the recombinant gene. The benefits of the antibody in the purification of protein C include the specificity of protein C and not of activated protein C, and the unique Ca 2+ peptide binding specificity that allows the binding site to be protected when immobilized on the chromatographic substrate. In vivo, the antibody has been demonstrated to inhibit tumor growth. The antibody can also be used to promote coagulation in patients who have high levels of factor VTII inhibitors.

NO90903788A 1988-12-30 1990-08-29 MONOCLONAL ANTIBODY AGAINST PROTEIN C. NO903788L (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US29244788A 1988-12-30 1988-12-30
PCT/US1989/005851 WO1990007524A1 (en) 1988-12-30 1989-12-28 Monoclonal antibody against protein c

Publications (2)

Publication Number Publication Date
NO903788D0 NO903788D0 (en) 1990-08-29
NO903788L true NO903788L (en) 1990-10-29

Family

ID=26780202

Family Applications (1)

Application Number Title Priority Date Filing Date
NO90903788A NO903788L (en) 1988-12-30 1990-08-29 MONOCLONAL ANTIBODY AGAINST PROTEIN C.

Country Status (1)

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NO (1) NO903788L (en)

Also Published As

Publication number Publication date
NO903788D0 (en) 1990-08-29

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