NO20001420L - Procedure for controlling the competitiveness of a microbial strain - Google Patents
Procedure for controlling the competitiveness of a microbial strainInfo
- Publication number
- NO20001420L NO20001420L NO20001420A NO20001420A NO20001420L NO 20001420 L NO20001420 L NO 20001420L NO 20001420 A NO20001420 A NO 20001420A NO 20001420 A NO20001420 A NO 20001420A NO 20001420 L NO20001420 L NO 20001420L
- Authority
- NO
- Norway
- Prior art keywords
- strain
- competitiveness
- genetic marker
- pcr
- microbial
- Prior art date
Links
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Fremgangsmåte for selektiv overvåking av utviklingen og/eller konkurranseevnen til en mikrobestamme i et prøvemiljø, omfattende innføyelse av en selektiv påvisbar genetisk markør i stammen og tilsetting av den merkede stamme til prøvemiljøet og isolering av DNA eller RNA fra stammen fra prøvemiljøet og påvise mengden genetisk markør. Fremgangsmåten anvendes også for å utvelge, blant et mangfold av mikrobestammer, den stammen "som T et"utvalgt miljø"og under utvalgte betingelser har den høyeste evne til å overleve, dele seg og/eller uttrykke et utvalgt gen. Mengden genetisk markør påvises ved PCR omfattende en PCR-teknikk som er i stand til å påvise en markør bestående av en enkelt _ nukJeotidsubstitusjon, inkludert en teknikk der den første PCR-syklus omfatter anvendelse av en revers transkriptase. Fremgangsmåten er også nyttig for å bestemme identiteten og opprinnelsen til en mikrobestamme ved å innføye en genetisk markør omfattende en kodet beskjed.A method of selectively monitoring the development and / or competitiveness of a microbial in a sample environment, including inserting a selectively detectable genetic marker into the strain and adding the labeled strain to the sample environment and isolating DNA or RNA from the strain from the sample environment and detecting the amount of genetic marker . The method is also used to select, among a variety of microbes, the strain "which T has a" selected environment "and under selected conditions has the highest ability to survive, divide and / or express a selected gene. PCR comprising a PCR technique capable of detecting a marker consisting of a single nucleotide substitution, including a technique wherein the first PCR cycle involves the use of a reverse transcriptase. The method is also useful for determining the identity and origin of a microbial by inserting a genetic marker comprising a coded message.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
NO20001420A NO20001420L (en) | 1997-09-18 | 2000-03-17 | Procedure for controlling the competitiveness of a microbial strain |
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
NO974299A NO974299D0 (en) | 1997-09-18 | 1997-09-18 | Method for stable labeling of microorganisms |
PCT/IB1998/001411 WO1999014359A1 (en) | 1997-09-18 | 1998-09-11 | Method of monitoring the competitiveness of a microbial strain |
NO20001420A NO20001420L (en) | 1997-09-18 | 2000-03-17 | Procedure for controlling the competitiveness of a microbial strain |
Publications (2)
Publication Number | Publication Date |
---|---|
NO20001420D0 NO20001420D0 (en) | 2000-03-17 |
NO20001420L true NO20001420L (en) | 2000-05-16 |
Family
ID=26648783
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
NO20001420A NO20001420L (en) | 1997-09-18 | 2000-03-17 | Procedure for controlling the competitiveness of a microbial strain |
Country Status (1)
Country | Link |
---|---|
NO (1) | NO20001420L (en) |
-
2000
- 2000-03-17 NO NO20001420A patent/NO20001420L/en not_active Application Discontinuation
Also Published As
Publication number | Publication date |
---|---|
NO20001420D0 (en) | 2000-03-17 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
Bodrossy et al. | Development and validation of a diagnostic microbial microarray for methanotrophs | |
Hugenholtz et al. | Novel division level bacterial diversity in a Yellowstone hot spring | |
Santos et al. | Identification and phylogenetic sorting of bacterial lineages with universally conserved genes and proteins | |
Kozdroj et al. | Structural diversity of microorganisms in chemically perturbed soil assessed by molecular and cytochemical approaches | |
Horz et al. | Identification of major subgroups of ammonia-oxidizing bacteria in environmental samples by T-RFLP analysis of amoA PCR products | |
Chin et al. | Structure and function of the methanogenic archaeal community in stable cellulose-degrading enrichment cultures at two different temperatures (15 and 30 C) | |
Neretin et al. | Quantification of dissimilatory (bi) sulphite reductase gene expression in Desulfobacterium autotrophicum using real‐time RT‐PCR | |
ATE328115T1 (en) | MULTIPLEX GENOTYPING USING FLUORESCENCE-LABLED HYBRIDIZATION PROBE | |
DE60038015D1 (en) | METHOD BASED ON A POLYNUCLEOTIDE MATRIX FOR IDENTIFYING MICROORGANISMS | |
IN188176B (en) | ||
ATE377074T1 (en) | METHOD OF PROVIDING DNA SEQUENCES | |
US5948649A (en) | Method for identifying nucleic acid sequences from different biological sources through amplification of the same | |
De Gregorio et al. | The abundant class of nemis repeats provides RNA substrates for ribonuclease III in Neisseriae | |
DK0636187T3 (en) | Nucleotide sequences that hybridize specifically with a campylobacter jejuni genome nucleus sequence | |
DE69732926D1 (en) | PRIMERS AND PROBES FOR THE AMPLIFICATION, DETECTION AND TYPING OF MYCOPLASMA PNEUMONIAE | |
NO20001420L (en) | Procedure for controlling the competitiveness of a microbial strain | |
ATE554164T1 (en) | PRIMERS FOR NUCLEIC ACID AMPLIFICATION IN HOUSEHOLD GENE MRNA DETECTION AND TESTING METHODS USING THE PRIMERS | |
Kuhn et al. | Prevalence of pSmeSM11a-like plasmids in indigenous Sinorhizobium meliloti strains isolated in the course of a field release experiment with genetically modified S. meliloti strains | |
FR2683227B1 (en) | NUCLEOTIDE SEQUENCES HYBRIDIZING WITH THE BACTERIAL STRAINS OF THE MYCOBACTERIUM AVIUM-INTRACELLULARE COMPLEX, THEIR USE AS OLIGONUCLEOTIDE PRIMERS AND NUCLEIC PROBES. | |
Beeson et al. | Differentiation of plasmids in marine diazotroph assemblages determined by randomly amplified polymorphic DNA analysis | |
Nakatsukasa et al. | Transposon mediation allows a symbiotic plasmid of Rhizobium leguminosarum bv. trifolii to become a symbiosis island in Agrobacterium and Rhizobium | |
ATE486138T1 (en) | METHOD FOR DETECTING BACTERIA OF THE GENUS MYCOBACTERIUM (ACID-RESISTANT BACTERIA) | |
Yakimov et al. | U pstream‐independent ribosomal R NA amplification a nalysis (URA): a new approach to characterizing the diversity of natural microbial communities | |
Deming et al. | Search and discovery of microbial enzymes from thermally extreme environments in the ocean | |
DE69610933D1 (en) | METHOD AND REAGENTS FOR TYPING CLASS I HLA GENES |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
FC2A | Withdrawal, rejection or dismissal of laid open patent application |