NO20001420L - Procedure for controlling the competitiveness of a microbial strain - Google Patents

Procedure for controlling the competitiveness of a microbial strain

Info

Publication number
NO20001420L
NO20001420L NO20001420A NO20001420A NO20001420L NO 20001420 L NO20001420 L NO 20001420L NO 20001420 A NO20001420 A NO 20001420A NO 20001420 A NO20001420 A NO 20001420A NO 20001420 L NO20001420 L NO 20001420L
Authority
NO
Norway
Prior art keywords
strain
competitiveness
genetic marker
pcr
microbial
Prior art date
Application number
NO20001420A
Other languages
Norwegian (no)
Other versions
NO20001420D0 (en
Inventor
Peter Alestroem
Dag Lillehaug
Gerd Vegarud
Original Assignee
Microtag As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from NO974299A external-priority patent/NO974299D0/en
Application filed by Microtag As filed Critical Microtag As
Priority to NO20001420A priority Critical patent/NO20001420L/en
Publication of NO20001420D0 publication Critical patent/NO20001420D0/en
Publication of NO20001420L publication Critical patent/NO20001420L/en

Links

Landscapes

  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Fremgangsmåte for selektiv overvåking av utviklingen og/eller konkurranseevnen til en mikrobestamme i et prøvemiljø, omfattende innføyelse av en selektiv påvisbar genetisk markør i stammen og tilsetting av den merkede stamme til prøvemiljøet og isolering av DNA eller RNA fra stammen fra prøvemiljøet og påvise mengden genetisk markør. Fremgangsmåten anvendes også for å utvelge, blant et mangfold av mikrobestammer, den stammen "som T et"utvalgt miljø"og under utvalgte betingelser har den høyeste evne til å overleve, dele seg og/eller uttrykke et utvalgt gen. Mengden genetisk markør påvises ved PCR omfattende en PCR-teknikk som er i stand til å påvise en markør bestående av en enkelt _ nukJeotidsubstitusjon, inkludert en teknikk der den første PCR-syklus omfatter anvendelse av en revers transkriptase. Fremgangsmåten er også nyttig for å bestemme identiteten og opprinnelsen til en mikrobestamme ved å innføye en genetisk markør omfattende en kodet beskjed.A method of selectively monitoring the development and / or competitiveness of a microbial in a sample environment, including inserting a selectively detectable genetic marker into the strain and adding the labeled strain to the sample environment and isolating DNA or RNA from the strain from the sample environment and detecting the amount of genetic marker . The method is also used to select, among a variety of microbes, the strain "which T has a" selected environment "and under selected conditions has the highest ability to survive, divide and / or express a selected gene. PCR comprising a PCR technique capable of detecting a marker consisting of a single nucleotide substitution, including a technique wherein the first PCR cycle involves the use of a reverse transcriptase. The method is also useful for determining the identity and origin of a microbial by inserting a genetic marker comprising a coded message.

NO20001420A 1997-09-18 2000-03-17 Procedure for controlling the competitiveness of a microbial strain NO20001420L (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
NO20001420A NO20001420L (en) 1997-09-18 2000-03-17 Procedure for controlling the competitiveness of a microbial strain

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
NO974299A NO974299D0 (en) 1997-09-18 1997-09-18 Method for stable labeling of microorganisms
PCT/IB1998/001411 WO1999014359A1 (en) 1997-09-18 1998-09-11 Method of monitoring the competitiveness of a microbial strain
NO20001420A NO20001420L (en) 1997-09-18 2000-03-17 Procedure for controlling the competitiveness of a microbial strain

Publications (2)

Publication Number Publication Date
NO20001420D0 NO20001420D0 (en) 2000-03-17
NO20001420L true NO20001420L (en) 2000-05-16

Family

ID=26648783

Family Applications (1)

Application Number Title Priority Date Filing Date
NO20001420A NO20001420L (en) 1997-09-18 2000-03-17 Procedure for controlling the competitiveness of a microbial strain

Country Status (1)

Country Link
NO (1) NO20001420L (en)

Also Published As

Publication number Publication date
NO20001420D0 (en) 2000-03-17

Similar Documents

Publication Publication Date Title
Bodrossy et al. Development and validation of a diagnostic microbial microarray for methanotrophs
Hugenholtz et al. Novel division level bacterial diversity in a Yellowstone hot spring
Santos et al. Identification and phylogenetic sorting of bacterial lineages with universally conserved genes and proteins
Kozdroj et al. Structural diversity of microorganisms in chemically perturbed soil assessed by molecular and cytochemical approaches
Horz et al. Identification of major subgroups of ammonia-oxidizing bacteria in environmental samples by T-RFLP analysis of amoA PCR products
Chin et al. Structure and function of the methanogenic archaeal community in stable cellulose-degrading enrichment cultures at two different temperatures (15 and 30 C)
Neretin et al. Quantification of dissimilatory (bi) sulphite reductase gene expression in Desulfobacterium autotrophicum using real‐time RT‐PCR
ATE328115T1 (en) MULTIPLEX GENOTYPING USING FLUORESCENCE-LABLED HYBRIDIZATION PROBE
DE60038015D1 (en) METHOD BASED ON A POLYNUCLEOTIDE MATRIX FOR IDENTIFYING MICROORGANISMS
IN188176B (en)
ATE377074T1 (en) METHOD OF PROVIDING DNA SEQUENCES
US5948649A (en) Method for identifying nucleic acid sequences from different biological sources through amplification of the same
De Gregorio et al. The abundant class of nemis repeats provides RNA substrates for ribonuclease III in Neisseriae
DK0636187T3 (en) Nucleotide sequences that hybridize specifically with a campylobacter jejuni genome nucleus sequence
DE69732926D1 (en) PRIMERS AND PROBES FOR THE AMPLIFICATION, DETECTION AND TYPING OF MYCOPLASMA PNEUMONIAE
NO20001420L (en) Procedure for controlling the competitiveness of a microbial strain
ATE554164T1 (en) PRIMERS FOR NUCLEIC ACID AMPLIFICATION IN HOUSEHOLD GENE MRNA DETECTION AND TESTING METHODS USING THE PRIMERS
Kuhn et al. Prevalence of pSmeSM11a-like plasmids in indigenous Sinorhizobium meliloti strains isolated in the course of a field release experiment with genetically modified S. meliloti strains
FR2683227B1 (en) NUCLEOTIDE SEQUENCES HYBRIDIZING WITH THE BACTERIAL STRAINS OF THE MYCOBACTERIUM AVIUM-INTRACELLULARE COMPLEX, THEIR USE AS OLIGONUCLEOTIDE PRIMERS AND NUCLEIC PROBES.
Beeson et al. Differentiation of plasmids in marine diazotroph assemblages determined by randomly amplified polymorphic DNA analysis
Nakatsukasa et al. Transposon mediation allows a symbiotic plasmid of Rhizobium leguminosarum bv. trifolii to become a symbiosis island in Agrobacterium and Rhizobium
ATE486138T1 (en) METHOD FOR DETECTING BACTERIA OF THE GENUS MYCOBACTERIUM (ACID-RESISTANT BACTERIA)
Yakimov et al. U pstream‐independent ribosomal R NA amplification a nalysis (URA): a new approach to characterizing the diversity of natural microbial communities
Deming et al. Search and discovery of microbial enzymes from thermally extreme environments in the ocean
DE69610933D1 (en) METHOD AND REAGENTS FOR TYPING CLASS I HLA GENES

Legal Events

Date Code Title Description
FC2A Withdrawal, rejection or dismissal of laid open patent application