NO141656B - DEVICE FOR DIAGNOSTIC USE CONSISTING OF A PIPE WITH MULTIPLE CONTAINERS AND A SEALING INSTRUMENT - Google Patents
DEVICE FOR DIAGNOSTIC USE CONSISTING OF A PIPE WITH MULTIPLE CONTAINERS AND A SEALING INSTRUMENT Download PDFInfo
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- NO141656B NO141656B NO2147/73A NO214773A NO141656B NO 141656 B NO141656 B NO 141656B NO 2147/73 A NO2147/73 A NO 2147/73A NO 214773 A NO214773 A NO 214773A NO 141656 B NO141656 B NO 141656B
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- containers
- substrates
- rod
- container
- substrate
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Links
- 238000007789 sealing Methods 0.000 title 1
- 239000000758 substrate Substances 0.000 claims description 40
- 238000011081 inoculation Methods 0.000 claims description 9
- 239000002054 inoculum Substances 0.000 claims description 4
- 239000000463 material Substances 0.000 description 8
- 239000010410 layer Substances 0.000 description 7
- 235000015097 nutrients Nutrition 0.000 description 5
- 244000005700 microbiome Species 0.000 description 4
- 239000011241 protective layer Substances 0.000 description 4
- 241000588921 Enterobacteriaceae Species 0.000 description 3
- 238000001514 detection method Methods 0.000 description 3
- 241000894006 Bacteria Species 0.000 description 2
- KRKNYBCHXYNGOX-UHFFFAOYSA-K Citrate Chemical compound [O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O KRKNYBCHXYNGOX-UHFFFAOYSA-K 0.000 description 2
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 2
- SIKJAQJRHWYJAI-UHFFFAOYSA-N Indole Chemical compound C1=CC=C2NC=CC2=C1 SIKJAQJRHWYJAI-UHFFFAOYSA-N 0.000 description 2
- AHLPHDHHMVZTML-BYPYZUCNSA-N L-Ornithine Chemical compound NCCC[C@H](N)C(O)=O AHLPHDHHMVZTML-BYPYZUCNSA-N 0.000 description 2
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 description 2
- 239000004472 Lysine Substances 0.000 description 2
- AHLPHDHHMVZTML-UHFFFAOYSA-N Orn-delta-NH2 Natural products NCCCC(N)C(O)=O AHLPHDHHMVZTML-UHFFFAOYSA-N 0.000 description 2
- UTJLXEIPEHZYQJ-UHFFFAOYSA-N Ornithine Natural products OC(=O)C(C)CCCN UTJLXEIPEHZYQJ-UHFFFAOYSA-N 0.000 description 2
- 206010036790 Productive cough Diseases 0.000 description 2
- 239000008121 dextrose Substances 0.000 description 2
- 229960003104 ornithine Drugs 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 210000003802 sputum Anatomy 0.000 description 2
- 208000024794 sputum Diseases 0.000 description 2
- 241000589562 Brucella Species 0.000 description 1
- 241001112696 Clostridia Species 0.000 description 1
- KDXKERNSBIXSRK-YFKPBYRVSA-N L-lysine Chemical compound NCCCC[C@H](N)C(O)=O KDXKERNSBIXSRK-YFKPBYRVSA-N 0.000 description 1
- COLNVLDHVKWLRT-QMMMGPOBSA-N L-phenylalanine Chemical compound OC(=O)[C@@H](N)CC1=CC=CC=C1 COLNVLDHVKWLRT-QMMMGPOBSA-N 0.000 description 1
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 description 1
- 241000589516 Pseudomonas Species 0.000 description 1
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- 230000003851 biochemical process Effects 0.000 description 1
- 238000005842 biochemical reaction Methods 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000004202 carbamide Substances 0.000 description 1
- 239000011247 coating layer Substances 0.000 description 1
- 230000001627 detrimental effect Effects 0.000 description 1
- 238000007373 indentation Methods 0.000 description 1
- PZOUSPYUWWUPPK-UHFFFAOYSA-N indole Natural products CC1=CC=CC2=C1C=CN2 PZOUSPYUWWUPPK-UHFFFAOYSA-N 0.000 description 1
- RKJUIXBNRJVNHR-UHFFFAOYSA-N indolenine Natural products C1=CC=C2CC=NC2=C1 RKJUIXBNRJVNHR-UHFFFAOYSA-N 0.000 description 1
- 239000008101 lactose Substances 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 239000003209 petroleum derivative Substances 0.000 description 1
- COLNVLDHVKWLRT-UHFFFAOYSA-N phenylalanine Natural products OC(=O)C(N)CC1=CC=CC=C1 COLNVLDHVKWLRT-UHFFFAOYSA-N 0.000 description 1
- 230000000704 physical effect Effects 0.000 description 1
- 229920003023 plastic Polymers 0.000 description 1
- 230000009257 reactivity Effects 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 210000002700 urine Anatomy 0.000 description 1
- 229940099259 vaseline Drugs 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
- C12M41/36—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of biomass, e.g. colony counters or by turbidity measurements
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/08—Flask, bottle or test tube
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/34—Internal compartments or partitions
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
- C12M41/34—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of gas
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/801—Anerobic cultivation
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- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biotechnology (AREA)
- Genetics & Genomics (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Sustainable Development (AREA)
- Analytical Chemistry (AREA)
- Clinical Laboratory Science (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Examining Or Testing Airtightness (AREA)
Description
Nærværende oppfinnelse vedrører anordning for diagnostisk anvendelse, som består av et rør med flere beholdere og et podningsinstrument som går gjennom røret, og som på sin ene ende er utformet for å oppta podestoff, og som på sin andre ende er forsynt med et håndtak og at i det minste en beholder innehold- The present invention relates to a device for diagnostic use, which consists of a tube with several containers and an inoculation instrument that passes through the tube, and which is designed at one end to receive inoculation material, and which is provided with a handle at the other end and that at least one container contents
er et substrat som reagerer optimalt under anaerobe betingelser. is a substrate that reacts optimally under anaerobic conditions.
Det er kjent diagnostiske anordninger som har flere sub-stratholdige beholdere, hvorigjennom det går en trådstanq (podestang). I slike anordninger inneholder hver beholder et tradisjonelt testsubstrat som egner seg for bestemmelse av de biokjemiske egenskapene til en mikroorganisme. I de kjente anordningene blir en ende av stangen brakt i kontakt med materialer fra en prøve som f.eks. kan være sputum, blod osv. Podestangen blir deretter ført gjennom de forskjellige beholderne, slik at mikroorganismene, som forekommer på denne enden, tjener som inokulum eller podestoff for testsubstratene. There are known diagnostic devices which have several containers containing substrate, through which a wire rod (grafting rod) passes. In such devices, each container contains a traditional test substrate suitable for determining the biochemical properties of a microorganism. In the known devices, one end of the rod is brought into contact with materials from a sample such as e.g. may be sputum, blood, etc. The inoculum rod is then passed through the various containers, so that the micro-organisms present at this end serve as inoculum or inoculum for the test substrates.
Et problem, som forekommer i de kjente anordningene, består A problem, which occurs in the known devices, remains
i at mange testsubstrater, som reagerer optimalt under anaerobe betingelser er luftomfintlige. I slike anordninger kan luft trenge inn gjennom det efter fjerning av podestangen fri-gjorte rommet og/eller luften kan i hver beholder fylle ut det frie rommet over substratet. in that many test substrates, which react optimally under anaerobic conditions, are air-permeable. In such devices, air can penetrate through the space freed up after removal of the graft rod and/or the air can fill the free space above the substrate in each container.
Det er et av formålene med nærværende oppfinnelse å eliminere ulempene hos de kjente anordningene, og dette oppnås ifølge oppfinnelsen ved at podningsinstrumentet har en innskjæring mellom sine ender og at beholderne med substratene, som reagerer optimalt under anaerobe betingelser, er anordnet ved den ene enden av røret, idet avstanden mellom innskjæringen og den ene enden av podningsinstrumentet er bare litt større enn summen av avstandene mellom veggene til beholderne med substratene som reagerer optimalt under anaerobe betingelser. It is one of the purposes of the present invention to eliminate the disadvantages of the known devices, and this is achieved according to the invention in that the inoculation instrument has a cut between its ends and that the containers with the substrates, which react optimally under anaerobic conditions, are arranged at one end of the tube, the distance between the incision and one end of the inoculation instrument being only slightly greater than the sum of the distances between the walls of the containers with the substrates that react optimally under anaerobic conditions.
Ethvert substrat, på hvilke aerobe betingelser innvirker Any substrate, on which aerobic conditions affect
skadelig, blir tildekket med et ikke-porøst sjikt. På denne harmful, is covered with a non-porous layer. On this
måte sikres reaksjonsdyktighetene hos de substrater som reagerer optimalt under anaerobe betingelser ved kombinasjon av overtrekks-sjiktet, som fortrinnsvis er et vokssjikt med den innskårete podestangen. way, the reactivity of the substrates that react optimally under anaerobic conditions is ensured by combining the coating layer, which is preferably a wax layer with the incised graft rod.
Oppfinnelsen skal i det følgende anskueliggjøres ved hjelp av utførelseseksempler under henvisning til vedlagte tegninger, hvorav: In the following, the invention shall be illustrated by means of design examples with reference to the attached drawings, of which:
fig. 1 viser et sideriss av en anordning ifølge oppfinnelsen, fig. 1 shows a side view of a device according to the invention,
fig. 2 viser et bilde av podestangen, fig. 2 shows a picture of the graft rod,
fig. 3 viser anordningen efter poding. fig. 3 shows the device after grafting.
Anordningen ifølge oppfinnelsen anskueliggjøres ved hjelp av The device according to the invention is visualized by means of
fig. 1. Anordningen består av et rør 2 som består av gjennom-siktig kunststoff. Rørets ender er forsynt med skruehoder 3 og 4. Røret 2 er oppdelt i 8 beholdere som rommer næringssubstrater, antydet ved hjelp av strekede linjer. Selvfølge-lig kan røret 2 også inneholde mer eller mindre enn 8 beholdere. Uansett hvor stort antallet beholdere kan være så inneholder fig. 1. The device consists of a tube 2 which consists of transparent plastic. The ends of the tube are provided with screw heads 3 and 4. The tube 2 is divided into 8 containers that hold nutrient substrates, indicated by dashed lines. Of course, the tube 2 can also contain more or less than 8 containers. No matter how large the number of containers may be so contains
hver beholder et bestemt næringssubstrat for påvisning av en bestemt mikroorganisme. each container contains a specific nutrient substrate for the detection of a specific microorganism.
Veggene, som begrenser beholderne, oppviser alle en åpning. Disse åpningene befinner seg alle på en aksial linje, og således kan en stang eller en tråd 5 dras gjennom. En ende av stangen 5 rager ut av en ende av roret 2, og er forsynt med en innpodningsdel 6 som kan utgjore enden 9 av'stangen 5. Den andre enden til stangen 5 rager ut av den andre enden til roret 2 og er forsynt med et håndtak 7. Stangen 5 oppviser en innskjæring 8. The walls, which limit the containers, all have an opening. These openings are all located on an axial line, and thus a rod or a wire 5 can be pulled through. One end of the rod 5 protrudes from one end of the rudder 2, and is provided with a grafting part 6 which can form the end 9 of the rod 5. The other end of the rod 5 protrudes from the other end of the rudder 2 and is provided with a handle 7. The rod 5 has an indentation 8.
Ved bruk av anordningen blir innpodningsdel 6 brakt i kontakt When using the device, grafting part 6 is brought into contact
med isolerte materialer av en prøve, som f.eks. urin eller with isolated materials of a sample, such as urine or
sputum. Deretter griper man håndtaket 7 og drar stangen 5 gjennom roret 2. Ved innpodningsdelehs gjennomgang gjennom beholderen poder denne innpodningsdel 6 de i denne beholderen nærværende næringssubstrater. Efter en bestemt tid blir hver beholder visuelt undersokt med hensyn til de biokjemiske forandringene som anskueliggjøres ved at substratets karak-teristika endres, som f.eks. substratets farge. Oppkomsten av biokjemiske forandringer gir en diagnostisk betydningsfull informasjon. sputum. You then grasp the handle 7 and pull the rod 5 through the rudder 2. When the inoculation part passes through the container, this inoculation part 6 inoculates the nutrient substrates present in this container. After a certain time, each container is visually examined with regard to the biochemical changes that become apparent when the characteristics of the substrate change, such as e.g. the color of the substrate. The occurrence of biochemical changes provides diagnostically significant information.
I praksis inneholder anordningen ifblge oppfinnelsen substrater, såsom dekstrose, lysin eller ornitin eller lignende substrater i beholderne 10, 11 og 12. I slike substrater skjer den biokjemiske prosessen fortrinnsvis under anaerobe betingelser. Beholderne IO, 11 og 12 befinner seg ved anordningens ende, hvilken grenser opp til håndtaket 7, og er anordnet efter hverandre og alle beholderne som inneholder substrater som reagerer optimalt under anaerobe betingelser er tildekket med et vokssjikt 18. In practice, the device according to the invention contains substrates, such as dextrose, lysine or ornithine or similar substrates in the containers 10, 11 and 12. In such substrates, the biochemical process takes place preferably under anaerobic conditions. The containers 10, 11 and 12 are located at the end of the device, which borders up to the handle 7, and are arranged one behind the other and all the containers containing substrates that react optimally under anaerobic conditions are covered with a layer of wax 18.
Efter at innpodningsdelen 6 til podestangen 5 er trukket gjennom alle beholderne blir den igjen presset inn i roret 2. Podestangen 5 trenger gjennom de sentralt anordnede åpningene After the grafting part 6 of the grafting rod 5 has been pulled through all the containers, it is again pressed into the rudder 2. The grafting rod 5 penetrates through the centrally arranged openings
i sideveggene til beholderne inn i roret 2 inntil stangens ytre ende befinner seg i beholderen 13. I motsetning til beholderne 10, 11 og 12 inneholder beholderen 13 ikke noe substrat på hvilke aerobe betingelser innvirker skadelig. in the side walls of the containers into the rudder 2 until the outer end of the rod is in the container 13. In contrast to the containers 10, 11 and 12, the container 13 does not contain any substrate on which aerobic conditions have a detrimental effect.
Herved grenser den innskårete delen til den ytre veggen til beholderen 10. Stangen 5 blir derefter avbrutt ved den innskårete delen. Til slutt blir den del av stangen 5 som strekker seg fra den innskårete delen til håndtaket 7 fjernet. Hereby, the incised part adjoins the outer wall of the container 10. The rod 5 is then interrupted at the incised part. Finally, the part of the rod 5 which extends from the incised part to the handle 7 is removed.
Den forbedrede anordningen ifolge oppfinnelsen muliggjor i likhet med kjente anordninger en hurtig, sikker og enkel metode for påvisning av organismer som blant annet gram-negative bakterier av familien Enterobacteriaceae. Anordningen ifblge oppfinnelsen har imidlertid i forhold til kjente anordninger den fordel at man unngår de vanskeligheter som er forbundet med anvendelse av substrater som reagerer optimalt under anaerobe betingelser. The improved device according to the invention enables, like known devices, a fast, safe and simple method for detecting organisms such as gram-negative bacteria of the family Enterobacteriaceae. However, compared to known devices, the device according to the invention has the advantage that the difficulties associated with the use of substrates that react optimally under anaerobic conditions are avoided.
De i de forskjellige beholderne i anordningen ifolge oppfin- Those in the various containers in the device according to the invention
nelsen forekommende næringssubstrater kan av fagmannen utvelges blant mange næringssubstrater som egner seg for formålet ifolge nærværende oppfinnelse. nelsen nutrient substrates can be selected by the person skilled in the art from among many nutrient substrates that are suitable for the purpose according to the present invention.
De eneste krav som må stilles til test-substratene er at de The only requirements that must be placed on the test substrates are that they
efter poding med bestemte bakterier gjennomgår kjemiske forandringer som kan iakttas i det gjennomsiktige roret. Disse forandringer muliggjor iakttagelsen av diagnostisk betydnings-fulle egenskaper. after inoculation with certain bacteria undergo chemical changes that can be observed in the transparent tube. These changes enable the observation of diagnostically significant characteristics.
Ifolge en foretrukket utfbrélsesform inneholder anordningen According to a preferred embodiment, the device contains
ifblge oppfinnelsen dekstrose (beholder IO), lysin (11) og according to the invention dextrose (contains IO), lysine (11) and
ornitin (12). Disse substrater reagerer biokjemisk optimalt i fravær av luft. Videre kan anordningen inneholde H2S/indol i beholder 13; laktose (14), fenylalanin-dulicitol (15), urea (16), citrat (17) eller i en egnet mengde beholdere, visse substrater som reagerer i nærvær av luft. ornithine (12). These substrates react optimally biochemically in the absence of air. Furthermore, the device can contain H2S/indole in container 13; lactose (14), phenylalanine dulicitol (15), urea (16), citrate (17) or, in a suitable quantity of containers, certain substrates which react in the presence of air.
Som det allerede fremgår~av det beskrevne er innskjæringen As is already evident~from what has been described, the incision is
i stangen 5 et egnet middel for å sikre at de forandringer som foregår i substratene ikke påvirkes av aerobe betingelser. in rod 5 a suitable means to ensure that the changes that take place in the substrates are not affected by aerobic conditions.
Fblgelig består fordelen ifølge nærværende oppfinnelse Accordingly, the advantage according to the present invention remains
i anvendelsen av en innskåret og således avbrytbar podestang, hvorved denne podestang kan avbrytes og en del av denne igjen kan innfores i anordningen. På denne måten minskes muligheten for inntrengning av luft i beholderen som inneholder luftbm-fintlige substrater. in the use of an incised and thus interruptible grafting rod, whereby this grafting rod can be interrupted and part of it can again be inserted into the device. In this way, the possibility of air ingress into the container containing air-sensitive substrates is reduced.
For at substratene i beholderne IO, 11 og 12 ennå bedre skal So that the substrates in containers IO, 11 and 12 will be even better
kunne skånes for kontakt med luft kan de være tildekket med et beskyttende sjikt. Som beskyttende sjikt foretrekkes et vokssjikt. Som anskueliggjort ved hjelp av fig. 1 oa 3 blir i en beholder substratet fullstendig tildekket med vokssjiktet, og could be spared from contact with air, they can be covered with a protective layer. A wax layer is preferred as a protective layer. As illustrated by means of fig. 1 and oa 3, in a container the substrate is completely covered with the wax layer, and
dette vokssjikt ligger således an mot alle fire vegger til beholderen. Selv om voks foretrekkes som materiale for det beskyttende sjiktet så egner også andre materialer seg naturligvis for dette formål. Som for dette formål egnede materialer kan nevnes jordoljedestillater, såsom vaselin, kosmolin osv. Materialet i det beskyttende sjiktet må tildekke substratet fullstendig, være boyelig, ikke-porbst, og i tilfelle voks anvendes må denne være flytende over 65°C og fast under 50°C. this wax layer thus rests against all four walls of the container. Although wax is preferred as a material for the protective layer, other materials are naturally also suitable for this purpose. Suitable materials for this purpose include petroleum distillates, such as vaseline, cosmolin, etc. The material in the protective layer must cover the substrate completely, be flexible, non-porous, and if wax is used, it must be liquid above 65°C and solid below 50 °C.
Selv om anordningen er anskueliggjort med 3 "anaerobe" og 5 "aerobe" materialer, kan selvfølgelig fler eller færre enn 8 beholdere og fler eller færre enn 3 "anaerobe" substrater anvendes. Den eneste betingelse for en slik endring består, i at den innskårete delen til stangen 5 er slik anordnet at den , ved brytning av stangens 5 avbrutte del fullstendig går gjennom alle veggene til beholderne som inneholder "anaerobe" V'" substrater, (disse substrater er fullstendig tildekket med et.vokssjikt). I motsetning hertil går den avbrutte delen ikke fullstendig gjennom en beholder som inneholder "aerobt" substrat. Although the device is illustrated with 3 "anaerobic" and 5 "aerobic" materials, of course more or fewer than 8 containers and more or fewer than 3 "anaerobic" substrates can be used. The only condition for such a change is that the incised part of the rod 5 is arranged in such a way that, upon breaking the interrupted part of the rod 5, it completely passes through all the walls of the containers containing "anaerobic" V'" substrates, (these substrates is completely covered by a layer of wax).In contrast, the interrupted portion does not pass completely through a container containing "aerobic" substrate.
I anordningen kan det som allerede nevnt anyend.es substrat In the device, as already mentioned, the substrate can be anyend.es
for påvisning av mikroorganismene av familien Enterobacteriaceae. Det er imidlertid selvfølgelig for én fagmann at også andre organismer enn de som tilhører familien Enterobacteriaceae på lignende måte kan påvises ved anvendelse av anordningen ifølge oppfinnelsen. Som eksempler på andre organismer kan man nevne Clostridia, Pseudomonas og Brucella. De eneste krav som for the detection of the microorganisms of the Enterobacteriaceae family. However, it is of course for a person skilled in the art that organisms other than those belonging to the family Enterobacteriaceae can also be detected in a similar way by using the device according to the invention. Examples of other organisms include Clostridia, Pseudomonas and Brucella. The only requirements that
stilles på et testsubstrat består i at det skal være fast under 50°C og tillate påvisning av <p>atogene organismer ved biokjemisk reaksjon, dvs. oppvise en forandring med hensyn til fysikalske egenskaper, og at disse forandringer kan iakttas. placed on a test substrate consists in that it must be solid below 50°C and allow the detection of <p>athogenic organisms by biochemical reaction, i.e. show a change with regard to physical properties, and that these changes can be observed.
Claims (1)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US25698472A | 1972-05-25 | 1972-05-25 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| NO141656B true NO141656B (en) | 1980-01-07 |
| NO141656C NO141656C (en) | 1980-04-16 |
Family
ID=22974412
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| NO2147/73A NO141656C (en) | 1972-05-25 | 1973-05-24 | DEVICE FOR DIAGNOSTIC USE CONSISTING OF A PIPE WITH MULTIPLE CONTAINERS AND A SEALING INSTRUMENT |
Country Status (18)
| Country | Link |
|---|---|
| US (1) | US3784448A (en) |
| JP (1) | JPS5422035B2 (en) |
| AT (1) | AT330338B (en) |
| AU (1) | AU456457B2 (en) |
| BE (1) | BE799954A (en) |
| CA (1) | CA995569A (en) |
| CH (1) | CH582748A5 (en) |
| CS (1) | CS172256B2 (en) |
| DD (1) | DD106200A5 (en) |
| DK (1) | DK131581C (en) |
| FR (1) | FR2185380B1 (en) |
| GB (1) | GB1434092A (en) |
| IL (1) | IL42150A (en) |
| IT (1) | IT987390B (en) |
| NL (1) | NL156443B (en) |
| NO (1) | NO141656C (en) |
| SE (1) | SE389127B (en) |
| SU (1) | SU539540A3 (en) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3960658A (en) * | 1974-09-23 | 1976-06-01 | Centaur Chemical Co. | Multi-media petri dish |
| CA1059592A (en) * | 1976-07-26 | 1979-07-31 | Harold J. Ostapovitch | Box terminal for card edge receptacles in telecommunications systems and the like |
| US4235963A (en) * | 1979-05-11 | 1980-11-25 | Hansen Joe C | Sampling and identifying apparatus and method |
| US4318994A (en) * | 1979-08-30 | 1982-03-09 | Mcdonnell Douglas Corporation | Enterobacteriaceae species biochemical test card |
| FR2587722B1 (en) * | 1985-09-25 | 1988-05-20 | Rhone Poulenc Agrochimie | METHOD FOR DIAGNOSING FUNGAL DISEASES OF PLANTS AND DEVICE FOR CARRYING OUT SAID METHOD |
| JP2686199B2 (en) * | 1992-01-28 | 1997-12-08 | 矢崎総業株式会社 | Female terminal fitting |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3205151A (en) * | 1962-04-17 | 1965-09-07 | Hollister Inc | Inoculation device and method |
| FR1418656A (en) * | 1964-12-23 | 1965-11-19 | Hollister Inc | Apparatus and method for inoculating test media |
| US3308039A (en) * | 1966-02-15 | 1967-03-07 | Allergan Pharma | Disposable culturing device |
-
1972
- 1972-05-25 US US00256984A patent/US3784448A/en not_active Expired - Lifetime
-
1973
- 1973-04-30 IL IL42150A patent/IL42150A/en unknown
- 1973-05-01 GB GB2067873A patent/GB1434092A/en not_active Expired
- 1973-05-03 CH CH629473A patent/CH582748A5/xx not_active IP Right Cessation
- 1973-05-04 CA CA170,460A patent/CA995569A/en not_active Expired
- 1973-05-04 AU AU55268/73A patent/AU456457B2/en not_active Expired
- 1973-05-11 IT IT24004/73A patent/IT987390B/en active
- 1973-05-14 AT AT421373A patent/AT330338B/en not_active IP Right Cessation
- 1973-05-16 NL NL7306816.A patent/NL156443B/en unknown
- 1973-05-21 SU SU1921996A patent/SU539540A3/en active
- 1973-05-21 JP JP5667473A patent/JPS5422035B2/ja not_active Expired
- 1973-05-23 CS CS3708A patent/CS172256B2/cs unknown
- 1973-05-24 DK DK286273A patent/DK131581C/en active
- 1973-05-24 NO NO2147/73A patent/NO141656C/en unknown
- 1973-05-24 DD DD171040A patent/DD106200A5/xx unknown
- 1973-05-24 SE SE7307350A patent/SE389127B/en unknown
- 1973-05-24 BE BE131451A patent/BE799954A/en not_active IP Right Cessation
- 1973-05-25 FR FR7319057A patent/FR2185380B1/fr not_active Expired
Also Published As
| Publication number | Publication date |
|---|---|
| NL156443B (en) | 1978-04-17 |
| CH582748A5 (en) | 1976-12-15 |
| SU539540A3 (en) | 1976-12-15 |
| IL42150A (en) | 1975-11-25 |
| NL7306816A (en) | 1973-11-27 |
| JPS4970487A (en) | 1974-07-08 |
| DD106200A5 (en) | 1974-06-05 |
| AU456457B2 (en) | 1974-12-19 |
| GB1434092A (en) | 1976-04-28 |
| US3784448A (en) | 1974-01-08 |
| ATA421373A (en) | 1975-09-15 |
| FR2185380A1 (en) | 1974-01-04 |
| DE2322529A1 (en) | 1973-12-13 |
| SE389127B (en) | 1976-10-25 |
| NO141656C (en) | 1980-04-16 |
| BE799954A (en) | 1973-11-26 |
| DE2322529B2 (en) | 1975-06-05 |
| FR2185380B1 (en) | 1976-06-11 |
| CS172256B2 (en) | 1976-12-29 |
| DK131581B (en) | 1975-08-04 |
| AU5526873A (en) | 1974-11-07 |
| CA995569A (en) | 1976-08-24 |
| AT330338B (en) | 1976-06-25 |
| IL42150A0 (en) | 1973-06-29 |
| JPS5422035B2 (en) | 1979-08-03 |
| IT987390B (en) | 1975-02-20 |
| DK131581C (en) | 1976-01-05 |
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