NL7600012A - Compsn for determining urea in biological fluids - comprising urease, indicator dye and mixed buffers - Google Patents

Compsn for determining urea in biological fluids - comprising urease, indicator dye and mixed buffers

Info

Publication number
NL7600012A
NL7600012A NL7600012A NL7600012A NL7600012A NL 7600012 A NL7600012 A NL 7600012A NL 7600012 A NL7600012 A NL 7600012A NL 7600012 A NL7600012 A NL 7600012A NL 7600012 A NL7600012 A NL 7600012A
Authority
NL
Netherlands
Prior art keywords
compsn
urease
urea
biological fluids
buffers
Prior art date
Application number
NL7600012A
Other languages
Dutch (nl)
Original Assignee
Michael Moon Ki Chang
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Michael Moon Ki Chang filed Critical Michael Moon Ki Chang
Priority to NL7600012A priority Critical patent/NL7600012A/en
Publication of NL7600012A publication Critical patent/NL7600012A/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/58—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving urea or urease

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

A new compsn. for determining urea level in biological fluids comprises urease, buffers and an indicator dye, the buffers being mixed and comprising >=1 buffer which increases in pH as the temp. increases and >=1 other buffer which decreases in pH with the temp. increase. The compsn. may also contain stabilisers. The urea level in urea-contg. fluids is determined by adding the above compsn. and measuring the absorbence of the soln. just after the urease has catalysed the total conversion of urea to ammonia. The method of determining urea is simpler and faster to perform than the previously known urease methods, it does not require elevated temps. for reaction, utilises harmless chemicals and is quantitative.
NL7600012A 1976-01-02 1976-01-02 Compsn for determining urea in biological fluids - comprising urease, indicator dye and mixed buffers NL7600012A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
NL7600012A NL7600012A (en) 1976-01-02 1976-01-02 Compsn for determining urea in biological fluids - comprising urease, indicator dye and mixed buffers

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
NL7600012A NL7600012A (en) 1976-01-02 1976-01-02 Compsn for determining urea in biological fluids - comprising urease, indicator dye and mixed buffers

Publications (1)

Publication Number Publication Date
NL7600012A true NL7600012A (en) 1977-07-05

Family

ID=19825392

Family Applications (1)

Application Number Title Priority Date Filing Date
NL7600012A NL7600012A (en) 1976-01-02 1976-01-02 Compsn for determining urea in biological fluids - comprising urease, indicator dye and mixed buffers

Country Status (1)

Country Link
NL (1) NL7600012A (en)

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