NL2033089B1 - Method of evaluating quality trait stability of wheat - Google Patents
Method of evaluating quality trait stability of wheat Download PDFInfo
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- NL2033089B1 NL2033089B1 NL2033089A NL2033089A NL2033089B1 NL 2033089 B1 NL2033089 B1 NL 2033089B1 NL 2033089 A NL2033089 A NL 2033089A NL 2033089 A NL2033089 A NL 2033089A NL 2033089 B1 NL2033089 B1 NL 2033089B1
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- 241000209140 Triticum Species 0.000 title claims abstract description 97
- 235000021307 Triticum Nutrition 0.000 title claims abstract description 97
- 238000000034 method Methods 0.000 title claims abstract description 21
- 238000009331 sowing Methods 0.000 claims abstract description 79
- 238000011156 evaluation Methods 0.000 claims abstract description 29
- 230000035558 fertility Effects 0.000 claims abstract description 24
- 238000002474 experimental method Methods 0.000 claims abstract description 18
- 238000012545 processing Methods 0.000 claims abstract description 8
- 235000019750 Crude protein Nutrition 0.000 claims abstract description 7
- 108010068370 Glutens Proteins 0.000 claims abstract description 7
- 238000004458 analytical method Methods 0.000 claims abstract description 7
- 235000021312 gluten Nutrition 0.000 claims abstract description 7
- 229920002472 Starch Polymers 0.000 claims abstract description 6
- 238000004062 sedimentation Methods 0.000 claims abstract description 6
- 235000019698 starch Nutrition 0.000 claims abstract description 6
- 239000008107 starch Substances 0.000 claims abstract description 6
- 235000013339 cereals Nutrition 0.000 claims abstract description 5
- 235000020985 whole grains Nutrition 0.000 claims abstract description 4
- 238000012360 testing method Methods 0.000 claims description 3
- 239000003337 fertilizer Substances 0.000 description 33
- 239000000523 sample Substances 0.000 description 3
- 238000009395 breeding Methods 0.000 description 2
- 230000001488 breeding effect Effects 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 230000007613 environmental effect Effects 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 1
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 1
- 239000004202 carbamide Substances 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 230000014509 gene expression Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000012372 quality testing Methods 0.000 description 1
- 230000001850 reproductive effect Effects 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 230000010473 stable expression Effects 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
- G01N21/35—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using infrared light
- G01N21/359—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using infrared light using near infrared light
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
- G01N21/35—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using infrared light
- G01N21/3563—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using infrared light for analysing solids; Preparation of samples therefor
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/84—Systems specially adapted for particular applications
- G01N2021/8466—Investigation of vegetal material, e.g. leaves, plants, fruits
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- Physics & Mathematics (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Genetics & Genomics (AREA)
- Environmental Sciences (AREA)
- Developmental Biology & Embryology (AREA)
- Botany (AREA)
- Analysing Materials By The Use Of Radiation (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The present disclosure discloses a method of evaluating guality trait stability of wheat, which includes following steps: Sl, preparing to—be—tested wheat samples and reference varieties before a normal sowing season of wheat; SZ, setting up the to—be— 5 tested wheat samples and the reference varieties in Sl into experiment groups according to cross—influence of sowing date factor, sowing density factor and fertility level factor, and keeping field management for each of the experiment groups consistent; and S3, non—destructively detecting crude protein 10 content, starch, wet gluten, sedimentation value and grain hardness by a near—infrared whole grain quality analyzer after the samples in SZ are harvested, performing data processing and analysis, and, evaluating guality trait stability of the to—be— tested wheat samples. The evaluation method of the present 15 disclosure is simple and, convenient to operate, fast in speed, intuitive in results and strong in practicability, thereby the selection of wheat varieties can be effectively guided.
Description
METHOD OF EVALUATING QUALITY TRAIT STABILITY OF WHEAT
The present disclosure relates to the technical field of ag- ricultural breeding, in particular to a method of evaluating qual- ity trait stability of wheat.
The quality of wheat is affected by both genotype and envi- ronment. In different growing environments, quality traits of wheat are different, and the materials of different genotypes are also affected by the environment to a different degree. Therefore, the risk of wheat production can be reduced by finding and using varieties less affected by environmental factors and with stable genotype regulation and expression. At the same time, the selec- tion and utilization of varieties with stable expression of quali- ty traits is also crucial for processing enterprises, and is a matter of great concern to processing enterprises.
In recent years, more and more attention has been paid to the quality stability of wheat. The stability analysis method used is basically multi-year multi-point testing and quality testing. Alt- hough the effect is reliable, it is time-consuming and labor- intensive, and the progress of quality breeding and improvement is greatly limited. In view of this, it is of great significance to provide a rapid, reliable and comprehensive method for evaluating the quality stability of wheat.
In view of the above deficiencies, the purpose of the present disclosure is to provide a method of evaluating quality trait sta- bility of wheat, and the method has the characteristics of rapidi- ty, high accuracy and comprehensiveness.
In order to achieve the above object, the present disclosure adopts the following technical solutions:
A method of evaluating quality trait stability of wheat pro-
vided in the present disclosure includes following steps:
Sl, preparing to-be-tested wheat samples and reference varie- ties before a normal sowing season of the wheat; 32, setting up the to-be-tested wheat samples and the refer- ence varieties in 31 into experiment groups according to cross- influence of sowing date factor, sowing density factor and fertil- ity level factor, and keeping field management for each of the ex- periment groups consistent; and 33, non-destructively detecting crude protein content, starch, wet gluten, sedimentation value and grain hardness by a near-infrared whole grain quality analyzer after the samples in S2 are harvested, performing data processing and analysis, and evalu- ating quality trait stability of the to-be-tested wheat samples.
Further, the reference varieties in step S81 include recog- nized (or more influential) high-gluten certified varieties, medi- um-gluten certified varieties or weak-gluten certified varieties under local ecological conditions.
The purpose of setting reference varieties in the present disclosure is to set a scale. Quality traits are easily affected by environmental changes, and index values fluctuate greatly, therefore, the influence of uncertain factors can be effectively controlled and the objectivity and effectiveness of evaluation are ensured by selecting recognized varieties.
Further, in step S2, a test site is required to have uniform soil, fertilizer and water conditions, drill or broadcast, and fixed seedlings, the plot area is 1.5 to 3 square meters, which ensures that more than 0.5kg of seeds can be harvested, and exper- iments are designed according to random groups, and can also be adjusted according to needs and specific circumstances.
Further, at least two factors of the sowing date factor, the sowing density factor and the fertility level factor are included when setting up the experiment groups according to cross-influence in S2.
Further, the sowing date factor includes early sowing (20 to 60 days before normal sowing), normal sowing and late sowing (20 to 60 days after normal sowing), which can also be adjusted ac- cording to an actual local reproductive period.
Further, the sowing density factor includes high sowing den- sity (1.5 to 3.5 times of normal sowing density), normal sowing density and low sowing density (0.3 to 0.8 times of the normal sowing density), can also be adjusted according to the actual lo- cal sowing conditions, which can also be adjusted according to the actual local sowing situation.
Further, the fertility level factor includes high fertility level (1.5 to 2.5 times of normal fertility level), normal fertil- ity level and low fertility level (0.3 to 0.8 times of normal fer- tility level), which can also be adjusted according to local actu- al conditions.
Further, the data processing and analysis in S3 includes a mean value of amplitude variation and mean trend for both the to- be-tested wheat samples and the reference varieties under influ- ence of different factors.
Further, the evaluating quality trait stability of the to-be- tested wheat samples in S3 includes quality stability evaluation of a single experiment group and comprehensive quality stability evaluation.
Further, in the quality stability evaluation of a single ex- periment group, the quality trait stability of the to-be-tested wheat samples is measured by comparing differences of a same index between the to-be-tested wheat samples and the reference varieties under different treatment factors and levels, specifically includ- ing: regarding the stability as good when under influence of dif- ferent factors, the mean trend of the to-be-tested wheat samples and the reference varieties is consistent and a difference in a mean value of amplitude variation of the to-be-tested wheat sam- ples and the reference varieties is consistent; regarding the sta- bility as general when under the influence of different factors, the mean trend of the to-be-tested wheat samples and the reference varieties is consistent, but the difference in a mean value of am- plitude variation of the to-be-tested wheat samples and the refer- ence varieties is large (the mean value of amplitude variation ex- ceeds 1045); and regarding the stability as poor when under the influence of different factors, the mean trend of the to-be-tested wheat samples and the reference varieties is not consistent, and the difference in a mean value of amplitude variation of the to- be-tested wheat samples and the reference varieties is large (the mean value of amplitude variation exceeds 1045).
Further, the comprehensive quality stability evaluation is specifically: considering the comprehensive quality stability of to-be-tested wheat varieties to be good when the quality stability evaluation of all single experiment groups of the to-be-tested wheat varieties is good stability; considering the comprehensive quality stability of the to-be-tested wheat varieties to be good when the quality stability evaluation of all single experiment groups of the to-be-tested wheat varieties comprises good stabil- ity and general stability and does not comprise poor stability; considering the comprehensive quality stability of the to-be- tested wheat varieties to be general when the quality stability evaluation of all single experiment groups of the to-be-tested wheat varieties is general stability; and considering the compre- hensive quality stability of the to-be-tested wheat varieties to be poor when the quality stability evaluation of all single exper- iment groups of the to-be-tested wheat varieties comprises poor stability.
In conclusion, the present disclosure has the following ad- vantages: 1. In the method of evaluating quality trait stability of wheat provided in the present disclosure, the effects on crude protein content, starch, wet gluten, sedimentation value and grain hardness are investigated from the factors of sowing date, sowing density and fertility level, and the quality stability evaluation of a single experiment group and the comprehensive quality stabil- ity evaluation of the to-be-tested wheat samples are carried out, which is more conducive to the accuracy and stability of the eval- uation system. 2. The evaluation method of the present disclosure is simple and convenient to operate, fast in speed, intuitive in results and strong in practicability, thereby the selection of wheat varieties can be effectively guided.
In order to make the objectives, technical solutions and ad- vantages of the present disclosure clearer, the present disclosure will be further described in detail below with reference to the 5 embodiments. It should be understood that the specific embodiments described herein are only used to explain the present disclosure, but not to limit the present disclosure, that is, the described embodiments are only a part of the embodiments of the present dis- closure, rather than all the embodiments.
Thus, the following detailed description of the embodiments of the present disclosure provided are not intended to limit the scope of the present disclosure as claimed, but are merely repre- sentative of selected embodiments of the present disclosure. Based on the embodiments of the present disclosure, all other embodi- ments obtained by those skilled in the art without creative work will fall within the protection scope of the present disclosure.
A method of evaluating quality trait stability of wheat pro- vided in the present embodiment includes following steps:
Sl, preparing to-be-tested wheat samples (which is marked as sample 1) and reference varieties (which is specifically, Yangmai
No. 5) before a normal sowing season of the wheat; 32, setting up the to-be-tested wheat samples and the refer- ence varieties in Sl into experiment groups according to cross- influence of sowing date factor, sowing density factor and fertil- ity level factor, and keeping field management for each of the ex- periment groups consistent; wherein the sowing date factor specif- ically includes early sowing (45 days before normal sowing), nor- mal sowing (medium sowing) and late sowing (45 days after normal sowing); the sowing density factor specifically includes high sow- ing density (high density, which is 2 times of normal sowing den- sity), normal sowing density {medium density, which is with row length of 1m, row spacing of 25cm, and 25 plants per row) and low sowing density (low density, which is 0.5 times of the normal sow- ing density); the fertility level factor specifically includes high fertility level (high fertilizer, which is 2 times of normal fertility level), normal fertility level (medium fertilizer, basal fertilizer: special compound fertilizer for wheat 450kg/hm° and top dressing at jointing stage: urea 150kg/hm°} and low fertility level (low fertilizer, which is 0.5 times of the normal fertility lev- el); and the cross-influence is specifically that the sowing date factor, the sowing density factor and the fertility level factor intersect each other; and
S3, non-destructively detecting crude protein content, starch, wet gluten, sedimentation value and grain hardness by a near-infrared whole grain quality analyzer after the samples in S2 are harvested, performing data processing and analysis, and evalu- ating quality trait stability of the to-be-tested wheat samples, wherein the evaluating quality trait stability of the to-be-tested wheat samples includes quality stability evaluation of a single experiment group and comprehensive quality stability evaluation.
Wherein, in the quality stability evaluation of a single ex- periment group, the quality trait stability of the to-be-tested wheat samples is measured by comparing differences of a same index between the to-be-tested wheat samples and the reference varieties under different treatment factors and levels, specifically includ- ing: regarding the stability as good when under influence of dif- ferent factors, the mean trend of the to-be-tested wheat samples and the reference varieties is consistent and a difference in a mean value of amplitude variation of the to-be-tested wheat sam- ples and the reference varieties is consistent; regarding the sta- bility as general when under the influence of different factors, the mean trend of the to-be-tested wheat samples and the reference varieties is consistent, but the difference in a mean value of am- plitude variation of the to-be-tested wheat samples and the refer- ence varieties is large {the mean value of amplitude variation ex- ceeds 1045); and regarding the stability as poor when under the influence of different factors, the mean trend of the to-be-tested wheat samples and the reference varieties is not consistent, and the difference in a mean value of amplitude variation of the to- be-tested wheat samples and the reference varieties is large (the mean value of amplitude variation exceeds 1045). The comprehensive quality stability evaluation is specifically: considering the com- prehensive quality stability of to-be-tested wheat varieties to be good when the quality stability evaluation of all single experi- ment groups of the to-be-tested wheat varieties is good stability; considering the comprehensive quality stability of the to-be- tested wheat varieties to be good when the quality stability eval- uation of all single experiment groups of the to-be-tested wheat varieties comprises good stability and general stability and does not comprise poor stability; considering the comprehensive quality stability of the to-be-tested wheat varieties to be general when the quality stability evaluation of all single experiment groups of the to-be-tested wheat varieties is general stability; and con- sidering the comprehensive quality stability of the to-be-tested wheat varieties to be poor when the quality stability evaluation of all single experiment groups of the to-be-tested wheat varie- ties comprises poor stability. The specific results are shown in
Table 1 below.
Table 1 crude protein | starch con- wet gluten sedimentation influence factors content tent content value early sowing + high density + good general good good high fertilizer early sowing + high density + good good good general medium fertilizer early sowing + high density + good general good good low fertilizer early sowing + medium densi- good good good good ty + high fertilizer early sowing + medium densi- good good good good ty + medium fertilizer early sowing + medium densi- good good good good ty + low fertilizer early sowing + low density + good good good good high fertilizer early sowing + low density + good good good good medium fertilizer early sowing + low density + good good good good low fertilizer medium sowing + high density good general good good + high fertilizer medium sowing + high density good good good good + medium fertilizer medium sowing + high density good general good good + low fertilizer medium sowing + medium goad good good good density + high fertilizer medium sowing + medium good good good good density + medium fertilizer medium sowing + medium good good good good density + low fertilizer medium sowing + low density good good good general + high fertilizer medium sowing + low density good good good good + medium fertilizer medium sowing + low density good general good good + low fertilizer late sowing + high density + good good good good high fertilizer late sowing + high density + good good good good medium fertilizer late sowing + high density + good good good good low fertilizer late sowing + medium density goad good good good + high fertilizer late sowing + medium density good general good good + medium fertilizer late sowing + medium density good good good good + low fertilizer late sowing + low density + goad good good good high fertilizer late sowing + low density + good good good good medium fertilizer late sowing + low density + good good good good low fertilizer
Results: the stability of the crude protein content and the wet gluten content of the to-be- tested wheat varieties are good, but the comprehensive quality stability is general, that is, the quality trait stability of the to-be-tested wheat is general.
The above contents are only examples and descriptions of the present disclosure. Those skilled in the art can modify or supple- ment the described specific embodiments without creative work, or use similar methods to replace them, which still belong to the protection scope of this patent.
Claims (10)
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CN202111367284.4A CN114112986A (en) | 2021-11-18 | 2021-11-18 | Method for evaluating stability of quality characters of wheat |
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Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6208420B1 (en) * | 1999-03-02 | 2001-03-27 | Satake Corporation | Method and apparatus for estimating quality of grains |
CN109006469A (en) * | 2018-07-25 | 2018-12-18 | 南阳市农业科学院 | Wheat breed regionality quality phenotype identification method and wheat regionality Optimization Planting method |
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2021
- 2021-11-18 CN CN202111367284.4A patent/CN114112986A/en not_active Withdrawn
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Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6208420B1 (en) * | 1999-03-02 | 2001-03-27 | Satake Corporation | Method and apparatus for estimating quality of grains |
CN109006469A (en) * | 2018-07-25 | 2018-12-18 | 南阳市农业科学院 | Wheat breed regionality quality phenotype identification method and wheat regionality Optimization Planting method |
Non-Patent Citations (1)
Title |
---|
LOU HONGYAO ET AL: "Genome-wide association study of six quality-related traits in common wheat (Triticum aestivum L.) under two sowing conditions", THEORETICAL AND APPLIED GENETICS, vol. 134, no. 1, 5 November 2020 (2020-11-05), pages 399 - 418, XP037338964, ISSN: 0040-5752, DOI: 10.1007/S00122-020-03704-Y * |
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