NL2031045B1 - Calibration method for reducing effect of soil type on fluorescence intensity of polycyclic aromatic hydrocarbons - Google Patents
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- 239000002689 soil Substances 0.000 title claims abstract description 106
- 125000005575 polycyclic aromatic hydrocarbon group Chemical group 0.000 title claims abstract description 38
- 238000000034 method Methods 0.000 title claims abstract description 34
- 230000001603 reducing effect Effects 0.000 title claims abstract description 9
- 239000011159 matrix material Substances 0.000 claims abstract description 33
- 238000002189 fluorescence spectrum Methods 0.000 claims abstract description 31
- 238000001228 spectrum Methods 0.000 claims abstract description 30
- 230000000694 effects Effects 0.000 claims abstract description 24
- 125000005605 benzo group Chemical group 0.000 claims description 29
- GVEPBJHOBDJJJI-UHFFFAOYSA-N fluoranthene Chemical compound C1=CC(C2=CC=CC=C22)=C3C2=CC=CC3=C1 GVEPBJHOBDJJJI-UHFFFAOYSA-N 0.000 claims description 29
- 238000004364 calculation method Methods 0.000 claims description 8
- 230000005284 excitation Effects 0.000 claims description 7
- 238000004445 quantitative analysis Methods 0.000 claims description 6
- 238000000985 reflectance spectrum Methods 0.000 claims 2
- 238000005516 engineering process Methods 0.000 abstract description 10
- 238000001514 detection method Methods 0.000 abstract description 7
- YMWUJEATGCHHMB-UHFFFAOYSA-N Dichloromethane Chemical compound ClCCl YMWUJEATGCHHMB-UHFFFAOYSA-N 0.000 description 9
- GYFAGKUZYNFMBN-UHFFFAOYSA-N Benzo[ghi]perylene Chemical group C1=CC(C2=C34)=CC=C3C=CC=C4C3=CC=CC4=CC=C1C2=C43 GYFAGKUZYNFMBN-UHFFFAOYSA-N 0.000 description 8
- TXVHTIQJNYSSKO-UHFFFAOYSA-N BeP Natural products C1=CC=C2C3=CC=CC=C3C3=CC=CC4=CC=C1C2=C34 TXVHTIQJNYSSKO-UHFFFAOYSA-N 0.000 description 4
- YNPNZTXNASCQKK-UHFFFAOYSA-N phenanthrene Chemical compound C1=CC=C2C3=CC=CC=C3C=CC2=C1 YNPNZTXNASCQKK-UHFFFAOYSA-N 0.000 description 4
- 230000007613 environmental effect Effects 0.000 description 3
- 239000006004 Quartz sand Substances 0.000 description 2
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 2
- MWPLVEDNUUSJAV-UHFFFAOYSA-N anthracene Chemical compound C1=CC=CC2=CC3=CC=CC=C3C=C21 MWPLVEDNUUSJAV-UHFFFAOYSA-N 0.000 description 2
- 150000004945 aromatic hydrocarbons Chemical class 0.000 description 2
- 125000001997 phenyl group Chemical group [H]C1=C([H])C([H])=C(*)C([H])=C1[H] 0.000 description 2
- BBEAQIROQSPTKN-UHFFFAOYSA-N pyrene Chemical compound C1=CC=C2C=CC3=CC=CC4=CC=C1C2=C43 BBEAQIROQSPTKN-UHFFFAOYSA-N 0.000 description 2
- 206010007269 Carcinogenicity Diseases 0.000 description 1
- 206010016275 Fear Diseases 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 231100000260 carcinogenicity Toxicity 0.000 description 1
- 230000007670 carcinogenicity Effects 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 231100000025 genetic toxicology Toxicity 0.000 description 1
- 230000001738 genotoxic effect Effects 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 210000003734 kidney Anatomy 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 231100000299 mutagenicity Toxicity 0.000 description 1
- 230000007886 mutagenicity Effects 0.000 description 1
- 150000002894 organic compounds Chemical class 0.000 description 1
- 238000000643 oven drying Methods 0.000 description 1
- 239000002957 persistent organic pollutant Substances 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 238000003672 processing method Methods 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 210000002345 respiratory system Anatomy 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 239000010421 standard material Substances 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
- G01N21/35—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using infrared light
- G01N21/3563—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using infrared light for analysing solids; Preparation of samples therefor
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- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/27—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands using photo-electric detection ; circuits for computing concentration
- G01N21/274—Calibration, base line adjustment, drift correction
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
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- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
- G01N21/35—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using infrared light
- G01N21/359—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using infrared light using near infrared light
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- G01N21/64—Fluorescence; Phosphorescence
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Abstract
The present invention discloses a calibration method for reducing the effect of a soil type on the fluorescence intensity of polycyclic aromatic hydrocarbons. In the present invention, firstly, multiple 5 different types of soil specimens are prepared, and soil samples having the same concentration of polycyclic aromatic hydrocarbons are prepared from the soil specimens to form multiple experimental samples; then, by the combination of the fluorescence spectrum technology and the near-infrared diffuse reflection spectrum technology, the corresponding fluorescence intensity matrix F and calibrated matrix G are established respectively, and the aforementioned matrices are used for calibration to reduce the effect ofthe soil type on the fluorescence intensity ofthe PAHs. Adopting this method can quickly and effectively achieve calibration ofthe effect ofthe soil type on the fluorescence intensity ofthe polycyclic aromatic hydrocarbons, which provides theoretical and experimental bases forthe application ofthe fluorescence spectrum technology in the on-site detection ofthe polycyclic aromatic hydrocarbons in the soil.
Description
P35562NL00/MKO Title: CALIBRATION METHOD FOR REDUCING EFFECT OF SOIL TYPE ON FLUORESCENCE
TECHNICAL FIELD The present invention belongs to the field of detection methods, and relates to the detection of polycyclic aromatic hydrocarbons in soil, and in particular to a calibration method for reducing the effect of a soil type on the fluorescence intensity of polycyclic aromatic hydrocarbons.
BACKGROUND Polycyclic aromatic hydrocarbons (PAHS) refer to aromatic hydrocarbons containing two or more benzene rings. Because of its toxicity, genotoxicity, mutagenicity and carcinogenicity, it can cause various harms to human body, such as harms to the respiratory system, liver and kidney, and thus it is considered as a main organic pollutant that affects human health. Soil is the material basis of sustainable development of economic society, and protecting soil environment well is an important content for promoting ecological civilization construction and maintaining national ecological security. However, as an important environmental medium, the soil bears more than 90% of the environmental load of PAHS, and thus the pollution of the PAHs to soil is particularly prominent, and it is urgent to repair and control the PAHs in the soil. However first, we need to know the distribution and concentration of the PAHs in the soil definitely. Therefore, it has become one of the major problems that need to be solved urgently by environmental protection and agricultural departments to develop a convenient and rapid method for detecting the PAHs in the soil. A fluorescence spectrum technology has been widely applied in the detection of the PAHs in the soil because of its advantages of having high sensitivity and good selectivity, and being capable of achieving rapid on-site detection and the like. However, a soil type has an effect on the fluorescence characteristics of the PAHs, but how to effectively reduce its effect on the fluorescence intensity of the PAHs is essential for accurate quantitative analysis of the PAHs content in the soil by the fluorescence spectrum technology. Chinese invention patent application publication No. CN11073947 A discloses a calibration method for reducing the effect of a soil type on the fluorescence working curve of polycyclic aromatic hydrocarbons by a ratio of fluorescence intensity to near-infrared diffuse reflection intensity. Although this method can reduce the effect of the soil type on the fluorescence intensity of the PAHs to a certain extent, the effect is not obvious.
SUMMARY Aiming at the shortcomings existed in the prior art, the present invention provides a calibration method for reducing the effect of a soil type on the fluorescence intensity of polycyclic aromatic hydrocarbons, which is established by combining a fluorescence spectrum technology with a near- infrared diffuse reflection spectrum technology. This method provides theoretical and experimental 40 bases for on-site detection of the polycyclic aromatic hydrocarbons in soil. The present invention adopts the following technical solution.
-2- A calibration method for reducing the effect of a soil type on the fluorescence intensity of polycyclic aromatic hydrocarbons, includes the following steps: step 1: preparing multiple different types of soil specimens, and preparing soil samples having the same concentration of polycyclic aromatic hydrocarbons from the soil specimens; step 2: scanning the corresponding fluorescence spectrum and near-infrared diffuse reflection spectrum of each soil sample prepared in the step 1 to obtain the fluorescence spectrum and near- infrared diffuse reflection spectrum of each soil sample; step 3: for the fluorescence spectrum in the step 2, selecting a fluorescence spectrum band M for establishing quantitative analysis of the polycyclic aromatic hydrocarbons; step 4: for the near-infrared diffuse reflection spectrum in the step 2, selecting a near-infrared diffuse reflection band N for calibration; step 5: obtaining a corresponding fluorescence intensity matrix F from the fluorescence intensity at the quantitative fluorescence spectrum band M selected in the step 3; step 6: extracting the intensity of the near-infrared diffuse reflection band N of the step 4 to obtain a corresponding near-infrared diffuse reflection intensity matrix S; step 7: performing a 10-time multiplication calculation on the matrix S obtained in the step 6 to obtain a transformed near-infrared diffuse reflection intensity matrix E; step 8: performing calculation of an exponential function based on e of the matrix E obtained in the step 7 to obtain a corresponding calibrated matrix G; and step 9: calibrating the fluorescence intensity matrix F extracted in the step 5 by the calibrated matrix G obtained in the step 8, so as to reduce the effect of the soil type on the fluorescence intensity of the polycyclic aromatic hydrocarbons.
Furthermore, the aforementioned method further includes that after the step 9 is executed, different types of soil samples containing different concentrations of polycyclic aromatic hydrocarbons are formulated, and the steps 2-9 are repeated to verify the calibration method.
Preferably, in the step 2, the scanning range of the near-infrared diffuse reflection spectrum is 12,000-4,000 cmt; and the near-infrared diffuse reflection spectrum of each sample is scanned for 64 times, and an average spectrum is taken.
Preferably, in the step 2, an excitation wavelength of the fluorescence spectrum is 390 nm; and a fluorescence wavelength is 400-650 nm.
Preferably, in the step 1, the polycyclic aromatic hydrocarbon is benzo[ghilpyrene.
The present invention has the following beneficial effects.
In the present invention, multiple different types of soil specimens are randomly adopted, and corresponding soil samples are prepared by adding the same concentration of the polycyclic aromatic hydrocarbons into the different soil specimens to form multiple experimental specimens. Then, by the combination of the fluorescence spectrum technology and the near-infrared diffuse reflection spectrum technology, the corresponding fluorescence intensity matrix F and the calibrated matrix G are established respectively according to the selected characteristic band, so as to reduce the effect of the soil type on the fluorescence intensity of the polycyclic aromatic hydrocarbons. In this method, a 10- 40 time multiplication calculation is performed on the matrix S to obtain the matrix E, which is used for matching the magnitude of the matrix F. In practical application, various calculation methods are used
-3- for data processing, and it is found that the calibrated matrix G obtained by the calculation method of performing the exponential function based on e of the matrix E is a better processing method, and the calibrated result is more effective. Adopting this method can quickly and effectively achieve calibration of the effect of the soil type on the fluorescence intensity of the PAHs, which provides theoretical and experimental bases for the application of the fluorescence spectrum technology in the on-site detection of the polycyclic aromatic hydrocarbons in the soil.
BRIEF DESCRIPTION OF DRAWINGS FIG. 1 shows a fluorescence spectrum of different types of soil samples having the same concentration of benzo[ghilpyrene under the excitation of a light with a wavelength of 390 nm; FIG. 2 shows a near-infrared diffuse reflection spectrum of different types of soil samples having the same concentration of benzo[ghilpyrene; FIG. 3 shows the effect of the soil type on the fluorescence intensity of benzo[ghijpyrene at 487 nm before calibration; FIG. 4 shows the effect of the soil type on the fluorescence intensity of benzo[ghijpyrene at 487 nm after calibration; FIG. 5 shows a fluorescence spectrum of different types of soil samples having different concentrations of benzo[ghijpyrene under the excitation of a light with a wavelength of 390 nm; FIG. 6 shows a near-infrared diffuse reflection spectrum of different types of soil samples having different concentrations of benzo[ghilpyrene; FIG. 7 shows a fluorescence working curve of benzo[ghijpyrene in the soil before calibration; FIG. 8 shows a fluorescence working curve of benzo[ghilpyrene in the soil after calibration. FIG. 9 shows a fluorescence spectrum of different types of soil samples having different concentrations of fluoranthene under the excitation of a light with a wavelength of 390 nm; FIG. 10 shows a near-infrared diffuse reflection spectrum of different types of soil samples having different concentrations of fluoranthene; FIG. 11 shows a fluorescence working curve of fluoranthene in the soil before calibration; and FIG. 12 shows a fluorescence working curve of fluoranthene in the soil after calibration.
DETAILED DESCRIPTION The present invention will be further illustrated with reference to examples hereafter. The following examples are illustrative, rather than limiting, and cannot limit the claimed scope of the present invention. Polycyclic aromatic hydrocarbons are organic compounds formed by connecting two or more benzene rings, including more than 150 kinds of compounds such as anthracene, phenanthrene and fluoranthene. Taking benzo[ghijpyrene as an example, the calibration method of the present invention will be described in detail with reference to the accompanying drawings hereafter. A calibration method for reducing the effect of a soil type on the fluorescence intensity of a polycyclic aromatic hydrocarbons, included the following steps. 40 Step 1: multiple different types of soil specimens were prepared, and soil samples having the same concentration of polycyclic aromatic hydrocarbons were prepared from the soil specimens.
-4- In this example, the soil specimens in the step 1 were loessal soil of Shanxi Province and fluvo- aquic soil of Anhui Province, and the soil specimens were obtained by taking effective component analysis standard materials of the soil, and subjecting to operations of oven-drying and grinding. The aforementioned soil specimens met the corresponding requirements of GBWO74124a approved by the General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, and were purchased by the State center for standard matter of the People's Republic of China.
In this example, a certain amount of benzo[ghijpyrene powder (analytically pure, purchased from Shanghai Haohong Biomedical Technology Co., Ltd.) was taken, dissolved in dichloromethane, and shaken uniformly to prepare a solution in a beaker, the beaker was placed in an ultrasonic cleaner for 10 seconds to dissolve benzo[ghijpyrene fully in dichloromethane, and then the solution was evenly poured onto the surface of the soil in each culture dish, as guided with a glass rod. After dichloromethane was completely volatilized, the air-dried soil samples of benzo[ghilpyrene were ground to ensure that benzo[ghijpyrene was uniformly mixed in the soil, so as to prepare corresponding soil samples.
Step 2: the fluorescence spectrum and near-infrared diffuse reflection spectrum of each soil sample prepared in the step 1 were scanned to obtain the fluorescence spectrum and near-infrared diffuse reflection spectrum of each soil sample.
In this example, a LS-55 fluorescence spectrophotometer produced by American PE was adopted to scan the fluorescence spectra of the prepared soil samples, so as to obtain the fluorescence spectrum of each sample. The instrument parameters were set as follows: an excitation wavelength was 390 nm, a scanning wavelength range was 400-650 nm, a slit width of each of excitation and emission monochromators was 10 nm, and a scanning rate was 1,000 nm/min.
As shown in FIG. 1, there were two obvious characteristic fluorescence peaks of benzo[ghijpyrene in the soil, which were located at 487 nm and 516 nm respectively.
Meanwhile, the near-infrared diffuse reflection spectra of the prepared soil samples were scanned by a Fourier near-infrared spectrometer produced by American PE, so as to obtain the near- infrared diffuse reflection spectrum of each sample. The instrument parameters were set as follows: the scanning range was 12,000-4,000 cm’, the resolution was 8 cm’, each sample was scanned for 64 times, and the average spectrum was taken.
As shown in FIG. 2, it could be seen that there was difference in the diffuse reflection spectrum intensity of different types of soil samples having the same concentration of benzo[ghijpyrene in the range of 12,000-4,000 cm.
Step 3: for the fluorescence spectra of the 5 soil specimens of benzo[ghijpyrene in the step 2, a fluorescence spectrum band M for establishing quantitative analysis of the fluorescence intensity of benzo[ghijpyrene was selected.
According to the fluorescence spectrum of benzo[ghijpyrene in the soil in FIG. 1, the quantitative characteristic spectrum band M for quantitative analysis of the fluorescence intensity of benzo[ghijpyrene was determined as 487 nm.
40 Step 4: for the near-infrared diffuse reflection spectra of the 5 soil specimens of benzo[ghijpyrene in the step 2, a near-infrared diffuse reflection band N for calibrating the fluorescence intensity, was
-5- selected.
According to the near-infrared diffuse reflection spectrum of FIG. 2, the near-infrared diffuse reflection spectrum band for calibrating the fluorescence intensity of benzo[ghijpyrene was determined as 5,220 om™.
Step 5: the fluorescence intensity of the 5 soil specimens of benzo[ghijpyrene in the step 3 at 487 nm was extracted to obtain the corresponding fluorescence intensity matrix F. Step 6: the intensity of the near-infrared diffuse reflection band N of the step 4 was extracted to obtain a corresponding near-infrared diffuse reflection intensity matrix S. Step 7: a 10-time multiplication calculation was performed on the matrix S obtained in the step 6 to obtain a transformed near-infrared diffuse reflection intensity matrix E. Step 8: calculation of an exponential function based on e of the matrix E obtained in the step 7 was performed to obtain a corresponding calibrated matrix G.
Step 9: the fluorescence intensity matrix F extracted in the step 5 was calibrated by the calibrated matrix G obtained in the step 8, so as to reduce the effect of the soil type on the fluorescence intensity of the polycyclic aromatic hydrocarbons.
Five samples of fluvo-aquic soil of Anhui Province, fluvo-aquic soil of Anhui Province with quartz sand contents of 10% and 20%, loessial soil of Shanxi Province, and loessial soil of Shanxi Province with a quartz sand content of 10%, were numbered as 1, 2, 3, 4 and 5 sequentially. The fluorescence peak values of the samples before calibration were linearly fitted to the sample types. The linear fitting of the fluorescence peak values at 487 nm before calibration was as shown in FIG. 3, and the obtained linear equation was: Fi. =48.45C + 276.26 1) For comparison, the linear fitting of the fluorescence peak value at 487 nm after calibration was shown in FIG. 4, and the obtained linear equation was: 5 Fi. =0.53C +10.02 2) Obviously, after calibration, the variation amplitude of the fluorescence intensity with the soil type was decreased, and the maximum fluorescence intensity was only increased by 16.5% relative to the minimum fluorescence intensity. The results showed that the calibration method applied in the patent could reduce the effect of the soil type on the fluorescence intensity of the polycyclic aromatic hydrocarbons.
In order to verify the effectiveness of the applied calibration method, ten different soil types of soil samples of benzo[ghi]perylene with the equal concentration gradient were formulated, and the benzo[ghi]perylene concentrations were 0.4, 0.6, 0.8,1.0,1.2,1.4,1.6,1.8, 2.0 and 2.2 mg/g, respectively. The fluorescence spectra and near-infrared diffuse reflection spectra of the ten soil samples were collected, as shown in FIGs. 5 and 6.
The fluorescence intensity Fasz of benzo[ghilperylene at 487 nm was calibrated by the aforementioned calibration method proposed in this application, so as to obtain the calibrated fluorescence intensity Fcas7: Fcas7=Fas7/exp{(Rasss*“10) (3) 40 Working curves of the fluorescence intensities at 487 nm of the 10 soil samples with different
-6- concentrations of benzo[ghijpyrene before and after calibration versus the concentration of benzo[ghijperylene in the soil were established, as shown in FIGs. 7 and 8, and the corresponding curve equations were equation (4) and equation (5) respectively: F,. =161.33C +238.00 (4) F‚ =4.77C +4.84 (5) The fitting correlation coefficients R? before and after calibration were 0.793 and 0.938, respectively. The aforementioned results showed that as compared with the fluorescence intensity at 487 nm of benzo[ghi]perylene in the soil before calibration, the fluorescence intensity at 487 nm of benzo[ghilperylene in the soil after calibration had a better linear relationship with the concentration of benzo[ghi]perylene, which indicated that the proposed calibration method could effectively reduce the effect of the soil type on the fluorescence intensity of benzo[ghijperylene.
In order to verify that the applied calibration method was suitable for any of the polycyclic aromatic hydrocarbons, eight different soil types of soil samples of fluoranthene with different concentration gradients were prepared, and the fluoranthene concentrations were 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, 1.0 and
1.1 mg/g respectively. The fluorescence spectra and near-infrared diffuse reflection spectra of the eight soil samples were collected, as shown in FIGs. 9 and 10.
The fluorescence intensity F47s of fluoranthene at 476 nm was calibrated by the aforementioned calibration method proposed in this application, so as to obtain the calibrated fluorescence intensity Fears: Fcare=Fazs/exp(Raess*10) (6) Working curves of the fluorescence intensities at 476 nm of the 8 soil samples with different concentrations of fluoranthene before and after calibration versus the concentration of fluoranthene in the soil were established, as shown in FIGs. 11 and 12, and the corresponding curve equations were equation (7) and equation (8) respectively: I. =30.23C +146.04 (7) F‚ =1.31C+2.56 (8) By comparing the working curves before and after calibration, it was found that the multiple correlation coefficient R? of the calibrated working curve was 0.916, while the multiple correlation coefficient R2 of the uncalibrated working curve was 0.758. From the aforementioned results, it could be seen that there was a good linear relationship between the calibrated fluorescence intensity and fluoranthene concentration, which indicated that the calibration method applied in this patent could effectively reduce the effect of the soil type on the fluorescence intensity of fluoranthene.
It had been proven by experiments of different kinds and concentrations of polycyclic aromatic hydrocarbons that, for any kind of polycyclic aromatic hydrocarbons, including pyrene, phenanthrene, fluoranthene and the like in different types of soil, the effect of the different types of soil on the quantitative analysis of the working curves of the polycyclic aromatic hydrocarbons can be reduced by the calibration method established in this application.
40
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