NL2018090B1

NL2018090B1 - Composition for augmenting the selenium deposition in animal tissue

Info

Publication number: NL2018090B1
Application number: NL2018090A
Authority: NL
Inventors: Segers Ludo; Ketels Eddy
Original assignee: Orffa Additives B V
Priority date: 2016-12-28
Filing date: 2016-12-28
Publication date: 2018-07-06

Abstract

The current invention concerns a method for enhancing the selenium deposition in animal tissue, said method comprises the provision of one or more selenium sources to the feed and/or drinking water of animals, characterized in that said at least 20% of the externally added selenium is L-Selenomethionine.

Description

COMPOSITION FOR AUGMENTING THE SELENIUM DEPOSITION IN ANIMAL TISSUE

TECHNICAL FIELD

The invention pertains to the technical field of trace elements in animal feed.

BACKGROUND

Selenium is an essential trace element for both animals and human. Selenium is a key nutrient in animal nutrition and is crucial for an optimal antioxidant capacity, immune function and health status. Selenium in animal diets can be supplied via raw materials or via supplemented selenium. Selenium in feed can be added as organic or inorganic source. In practice, selenium is often added under the form of selenized yeast, which is the traditional way of supplementing selenium as organic form. However, selenized yeast is found to be show important variations from batch to batch, and as a result will exhibit variable effects when fed to animals.

Some compositions have tried to overcome this problem. BE1020050 for instance describes a feed additive which enhances the biological availability of selenium.

Via their metabolism, added selenium to the diet of animals is able to build a reserve in animal tissue and can also be transferred to offspring via placenta, milk and eggs. As such, this deposited selenium ensures a good anti-oxidative function in the animal. The selenium reserve in the body can also be mobilized for selenoprotein synthesis. An adequate selenium reserve in the body of the animal ensures an optimal selenium and anti-oxidant status at all times, even in times of stress or low feed intake.

Again, it was perceived that the source of added selenium may result in variations in the animal and especially in the deposition of selenium in tissue. Since tissue deposition has an important impact on the health of the animal, it is therefore important to maximize the deposition.

Hence, there remains a need in the art for a selenium source which enhances selenium deposition in animal tissue.

The present invention aims to resolve at least some of the problems mentioned above.

SUMMARY OF THE INVENTION

The present invention provides a method according to claim 1. The inventors of the current invention found that an accurate selection of the source of selenium can have a distinct impact on the selenium deposition in animal tissue, which on its turn has a positive health effect on the animal.

In particular the current inventions is defined by the following, not limitative embodiments. 1. A method for enhancing the selenium deposition in animal tissue, said method comprises the provision of one or more selenium sources to the feed and/or drinking water of animals, characterized in that said at least 20% of the externally added selenium is L-Selenomethionine. 2. Method according to the previous embodiment, characterized in that at least 50% of the added selenium is L-Selenomethionine. 3. Method according to one of the previous embodiments, characterized in that the added selenium is L-Selenomethionine. 4. Method according to any one of the previous embodiments, characterized in that said source of selenium is provided on a binder. 5. Method according to any one of the previous embodiments, characterized in that said total amount of externally added selenium to the feed and/or drinking water of animals lie between 0.1 and 0.5 ppm. 6. Method according to any of the previous embodiments, characterized in that said animals are chosen from the group of poultry, ruminants and pigs. 7. Use of a composition comprising one or more sources of selenium whereby at least 20% of the added selenium is L-Selenomethionine for enhancing the selenium deposition in animal tissue when provided to animals. 8. Use of a composition according to the previous embodiment, whereby the selenium source is L-selenomethionine.

DESCRIPTION OF FIGURES

Figure 1 shows a graph depicting the selenium deposition in broiler tissue muscle based on different selenium sources.

Figure 2 shows the linear correlation between levels of L-Selenomethionine and selenium deposition in muscle.

Figure 3 shows a graph depicting the selenium deposition in broiler tissue muscle based on different selenium sources.

DETAILED DESCRIPTION OF THE INVENTION

The present invention concerns a method for enhancing the selenium deposition in animal tissue. Selenium deposited in tissue can act as a good body reserve which has an anti-oxidative function and which can be mobilized for later selenoprotein synthesis. This body reserve of selenium will hence have a positive effect on the health of animals.

Unless otherwise defined, all terms used in disclosing the invention, including technical and scientific terms, have the meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. By means of further guidance, term definitions are included to better appreciate the teaching of the present invention.

As used herein, the following terms have the following meanings: "A", "an", and "the" as used herein refers to both singular and plural referents unless the context clearly dictates otherwise. By way of example, "a compartment" refers to one or more than one compartment. "About" as used herein referring to a measurable value such as a parameter, an amount, a temporal duration, and the like, is meant to encompass variations of +/-20% or less, preferably +/-10% or less, more preferably +/-5% or less, even more preferably +/-1% or less, and still more preferably +/-0.1% or less of and from the specified value, in so far such variations are appropriate to perform in the disclosed invention. However, it is to be understood that the value to which the modifier "about" refers is itself also specifically disclosed. "Comprise," "comprising," and "comprises" and "comprised of" as used herein are synonymous with "include", "including", "includes" or "contain", "containing", "contains" and are inclusive or open-ended terms that specifies the presence of what follows e.g. component and do not exclude or preclude the presence of additional, non-recited components, features, element, members, steps, known in the art or disclosed therein.

The recitation of numerical ranges by endpoints includes all numbers and fractions subsumed within that range, as well as the recited endpoints.

The expression "% by weight" (weight percent), here and throughout the description unless otherwise defined, refers to the relative weight of the respective component based on the overall weight of the formulation.

In a first aspect, the current invention is directed to a method for enhancing the selenium deposition in animal tissue, said method comprises the provision of one or more selenium sources to the feed and/or drinking water of animals, and whereby at least 20% of the total amount of externally added selenium is in the form of L-Selenomethionine. In a further preferred embodiment, at least 50% of the total amount of added selenium is in the form of L-Selenomethionine.

The inventors of the current invention found that the source of selenium added to the feed of animals has a large impact on the amount of selenium deposition in animal tissue. The form L-Selenomethionine is an organic form of selenium and was found to have a positive impact on selenium deposition in tissue, far more than any other known selenium source in the field.

The inventors of the current invention noticed a significant increase in selenium deposition in tissue if at least 20% of the provided selenium to the animal is L-Selenomethionine. Moreover, the amount of selenium deposition in animal tissue and specifically in muscle grew with an elevated level of L-Selenomethionine in the feed or drinking water of the animals.

The highest levels of selenium deposition in animal tissue were found when pure L-Selenomethionine was used as source of selenium.

The amount of L-Selenomethionine in the composition can be determined by methods known in the art such as HPLC-ICPMS after triple proteolytic digestion, which are described in the report of the EURL-FA and which are herein incorporated by reference.

In a further preferred embodiment, the total source of selenium provided to the animal will be between 0.1 and 0.5 ppm, calculated on the amount of feed or drinking water. While selenium is an essential trace element, the amount provided to the animal may not be too high, because of a risk of toxic effects. The amount of selenium in a product may be determined by use of the hydride generation atomic absorption spectrometry (HGAAS) after microwave digestion method (prEN 16159:2010).

In a further preferred embodiment, the selenium source will be added onto a binder, such as talc or chalk. In a further preferred embodiment, said composition is dust free.

In a further preferred embodiment, the composition according to the current invention which can be used as a feed additive and which comprises one or more sources of selenium of which at least 20% of said selenium source is L-Selenomethionine and whereby the total amount of selenium in the composition is between 0.1 and 0.25% w/w.

The method according to the current invention is particularly useful for animals chosen from the group of poultry, ruminants such as cattle, sheep and goats, horses and pigs. The method also works for small domestic animals such as cats, dogs, rabbits and hamsters.

In a further preferred embodiment, the methodology of the current invention is particularly useful for pigs and poultry such as broilers and laying hens.

In a second aspect, the current invention is also directed to the use of a composition comprising one or more sources of selenium whereby at least 20%, more preferably at least 50% of the total amount of added selenium is in the form of L-Selenomethionine for enhancing the selenium deposition in animal tissue when provided to animals. In a most preferred embodiment, the selenium source is solely and thus 100% L-selenomethionine.

The invention is further described by the following non-limiting examples which further illustrate the invention, and are not intended to, nor should they be interpreted to, limit the scope of the invention.

EXAMPLES

Example 1: Effect of the dietary selenium source on selenium deposition in broiler muscle tissue

Experiment 1 was conducted with the following sources of selenium: a. sodium selenium which is an inorganic source of selenium (NaSe) b. a yeast derived selenium with 26% of L-Selenomethionine (SeYeast low) c. a yeast derived selenium with 69% of L-Selenomethionine (SeYeast high) d. a source comprising of 100 % L-Selenomethionine

The feed of 130 broilers (Ross 308) was supplemented with one of the four compositions as described above. The treatment given consisted of six treatments with 4 replicates (pens) of 5 birds/pen. The total amount of selenium added to the feed was equal in all settings (0.2 ppm), only the amount of L-Selenomethionine varied (see Table 1).

Table 1

On day 0, 10 birds were sacrificed to determine the initial selenium in muscle tissue. On day 14, three birds per pen were sample for breast muscle and selenium deposition measurement. Selenium levels were determined by using ICP-MS (Inductively coupled plasma mass spectrometry).

Figure 1 depicts a graph which shows the amount of selenium deposition in relation to the source of selenium and concentration of L-Selenomethionine present in the selenium source (results from day 14). From the graph, it is clear that a higher concentration of L-Selenomethionine in the selenium source results in a higher selenium deposition in tissue. This is further evidenced by the graph in figure 2, whereby the selenium deposition in tissue is expressed as function of the added selenium in the form of L-Selenomethionine. As shown, there is a very strong linear correlation (with R2 0.7186).

The obtained results were repeated in a second broiler trial whereby two different organic selenium sources where used. One organic selenium source was seleno hydroxymethionine, which is the hydroxyl analogue of Selenomethionine (Se-OH-Met). The second source was a 100% L-Selenomethionine. Sodium selenite (NaSe) was used as inorganic source of selenium. Broilers (Ross 308) were divided in four groups and either treated with 0 ppm selenium (negative control), 0.2 ppm selenium from NaSe, 0.2 ppm Se-OH-Met and a fourth group with 100% L-Selenomethionine form.

On day 7 and 14 of the experiment, 6 birds per treatment were sampled form breast muscle. The initial selenium level in breast meat was determined on 6 birds euthanized on day 0. Selenium levels were determined by using ICP-MS (Inductively coupled plasma mass spectrometry).

From the graph depicted in figure 3 it is clear that the selenium deposition in the group supplemented with 100% L-Selenomethionine showed the highest deposition. Supplementing inorganic selenium decreased selenium in muscle. The differences observed between Se-OH-Met and L-Selenomethionine can possibly be explained by the fact that Se-OH-Met requires conversion in the body towards L-Selenomethionine, which is a drawback for selenium deposition.

Similar results were obtained when sources of selenium were added to pigs. Addition of L-Selenomethionine resulted in the highest selenium deposition in tissue (data not shown).

To conclude, failure to supplement animals with selenium from an (organic) source results in a decrease of selenium in muscle tissue over time. For organic sources of selenium, the level of L-Selenomethionine determines the efficacy if selenium deposition.

Claims

A method for increasing selenium deposition in animal tissue, comprising providing one or more sources of selenium to the feed and / or drinking water of the animals, characterized in that: - at least 20% of the externally added selenium is L-Selenomethionine; -the total content of externally added selenium in the feed and / or drinking water is included between 0.1 and 0.5 ppm.

Method according to claim 1, characterized in that at least 50% of the externally added selenium is L-Selenomethionine.

Method according to one of the preceding claims, characterized in that the externally added selenium is L-Selenomethionine.

Method according to one of the preceding claims, characterized in that the sources of selenium are bound on a support.

A method according to any one of the preceding claims, characterized in that the animals are poultry, ruminants or pigs.

Use of a composition comprising one or more sources of selenium in which at least 20% of the added selenium is derived from L-Selenomethionine, to increase the deposition of selenium in animal tissue.

Use according to claim 6, characterized in that the selenium source is L-Selenomethionine.