NL2011087C2 - Method and system for imaging a molecular strand. - Google Patents
Method and system for imaging a molecular strand. Download PDFInfo
- Publication number
- NL2011087C2 NL2011087C2 NL2011087A NL2011087A NL2011087C2 NL 2011087 C2 NL2011087 C2 NL 2011087C2 NL 2011087 A NL2011087 A NL 2011087A NL 2011087 A NL2011087 A NL 2011087A NL 2011087 C2 NL2011087 C2 NL 2011087C2
- Authority
- NL
- Netherlands
- Prior art keywords
- strand
- excitation
- depletion
- focus
- line
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Links
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Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N21/6456—Spatial resolved fluorescence measurements; Imaging
- G01N21/6458—Fluorescence microscopy
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/0004—Microscopes specially adapted for specific applications
- G02B21/002—Scanning microscopes
- G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
- G02B21/0036—Scanning details, e.g. scanning stages
- G02B21/0048—Scanning details, e.g. scanning stages scanning mirrors, e.g. rotating or galvanomirrors, MEMS mirrors
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/0004—Microscopes specially adapted for specific applications
- G02B21/002—Scanning microscopes
- G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
- G02B21/0052—Optical details of the image generation
- G02B21/0076—Optical details of the image generation arrangements using fluorescence or luminescence
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/16—Microscopes adapted for ultraviolet illumination ; Fluorescence microscopes
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/32—Micromanipulators structurally combined with microscopes
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules
Landscapes
- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Optics & Photonics (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Immunology (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Pathology (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Claims (15)
Priority Applications (6)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
NL2011087A NL2011087C2 (en) | 2013-07-03 | 2013-07-03 | Method and system for imaging a molecular strand. |
EP21199075.9A EP3957983B1 (en) | 2013-06-03 | 2014-06-02 | Method and system for imaging a molecular strand |
ES14731835T ES2897575T3 (es) | 2013-06-03 | 2014-06-02 | Método y sistema para formar imágenes de una hebra molecular |
EP14731835.6A EP3004848B1 (en) | 2013-06-03 | 2014-06-02 | Method and system for imaging a molecular strand |
US14/895,477 US9766180B2 (en) | 2013-06-03 | 2014-06-02 | Method and system for imaging a molecular strand |
PCT/NL2014/050351 WO2014196854A1 (en) | 2013-06-03 | 2014-06-02 | Method and system for imaging a molecular strand |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
NL2011087A NL2011087C2 (en) | 2013-07-03 | 2013-07-03 | Method and system for imaging a molecular strand. |
NL2011087 | 2013-07-03 |
Publications (1)
Publication Number | Publication Date |
---|---|
NL2011087C2 true NL2011087C2 (en) | 2015-01-06 |
Family
ID=49226463
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
NL2011087A NL2011087C2 (en) | 2013-06-03 | 2013-07-03 | Method and system for imaging a molecular strand. |
Country Status (1)
Country | Link |
---|---|
NL (1) | NL2011087C2 (nl) |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20100176307A1 (en) * | 2007-08-18 | 2010-07-15 | Max-Planck-Gesellschaft Zur Forderung Der Wissenschaften E.V. | STED-Fluorescent Light Microscopy with Two-Photon Excitation |
US20110201509A1 (en) * | 2008-10-10 | 2011-08-18 | Jonas Tegenfeldt | Method for the mapping of the local at/gc ratio along dna |
US20120002031A1 (en) * | 2008-12-02 | 2012-01-05 | The Regents Of The University Of California | Imaging Arrangement and Microscope |
-
2013
- 2013-07-03 NL NL2011087A patent/NL2011087C2/en not_active IP Right Cessation
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20100176307A1 (en) * | 2007-08-18 | 2010-07-15 | Max-Planck-Gesellschaft Zur Forderung Der Wissenschaften E.V. | STED-Fluorescent Light Microscopy with Two-Photon Excitation |
US20110201509A1 (en) * | 2008-10-10 | 2011-08-18 | Jonas Tegenfeldt | Method for the mapping of the local at/gc ratio along dna |
US20120002031A1 (en) * | 2008-12-02 | 2012-01-05 | The Regents Of The University Of California | Imaging Arrangement and Microscope |
Non-Patent Citations (3)
Title |
---|
DONNERT G ET AL: "Macromolecular-scale resolution in biological fluorescence microscopy", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES, NATIONAL ACADEMY OF SCIENCES, US, vol. 103, no. 31, 1 August 2006 (2006-08-01), pages 11440 - 11445, XP002507994, ISSN: 0027-8424, DOI: 10.1073/PNAS.0604965103 * |
GAEL MONERON ET AL: "Fast STED microscopy with continuous wave fiber lasers", OPTICS EXPRESS, vol. 18, no. 2, 12 January 2010 (2010-01-12), pages 1302 - 1309, XP055002828, ISSN: 1094-4087, DOI: 10.1364/OE.18.001302 * |
HELL S W ET AL: "BREAKING THE DIFFRACTION RESOLUTION LIMIT BY STIMULATED EMISSION: STIMULATED-EMISSION-DEPLETION FLUORESCENCE MICROSCOPY", OPTICS LETTERS, THE OPTICAL SOCIETY, vol. 19, no. 11, 1 June 1994 (1994-06-01), pages 780 - 782, XP000449464, ISSN: 0146-9592 * |
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